LOCUS       DASSQV010000001         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_745929, whole genome shotgun sequence.
ACCESSION   DASSQV010000001 DASSQV010000000
VERSION     DASSQV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745929"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..834
                     /locus_tag="VFL04_00005"
     CDS             97..834
                     /locus_tag="VFL04_00005"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HET7838107.1"
                     /translation="MTSEKLDSLSLEALYAVADKMGLDLPPGLERLFVVEEISEAIAE
                     DSEDRRAAHGEAVHVEEKKYSSPELGEIDADLDSSGAEAPVLETRYNETMIHALVRDP
                     SWAFAYWDLSDADQTALFGEDGGSALFLRVVELGGEDGRREYFDIPVSSEDHQWYINL
                     PRPGVRFRIDLCVRPGDSPVARIRVLARSNDVESPRQSLEPDRPLSERVIRLLELSGI
                     QELNIELARDENPQRILPARSGQGEGE"
     gene            847..>2363
                     /locus_tag="VFL04_00010"
     CDS             847..>2363
                     /locus_tag="VFL04_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HET7838108.1"
                     /translation="MQNGYLALVLNAHLPYVRRPDHPRFLEERWLFEALSETYLPLLR
                     LFRRLDEDSVPFHLTLNISPTLAAMLGDKVLGERYLDYLDAQLRLASAEEARLGSDPA
                     FGPLARLYAELYRADREDFDITYSRNILGAFDYFYKRGRIELITTGATHAFLPLFRDS
                     AEAVSAQVETAIVAHRKAFGKHPAGFWLPQLGWFPGAADILHAYNVQYTVVSTRGALL
                     GDPTPRNGSFAPVQCPNGLTVFIRDSGAVKEVWSETEGYPADPVYRDFYRDIGFDLPI
                     EDLSPYTEVGPTRIFTGFKYWAVTGKTEHKRVYDPAAASARAVEHAERFLEARRAAAL
                     AAGADMDRPPLMVCPYDAELFGHWWFEGPTWLEALFRKAAAEPSLRFVTLDEYHRTYP
                     ASQVSMPEFSSWGDGGYAEVWLDGSNDWIYRHVFKAAERMSELAERFPDESWLRERIL
                     NQAAREVLLSMCSDWALLMRAGKSATFARRQVEEPIANFGRIYDMLCSNTVSTEWL"
BASE COUNT          371 a          891 c          732 g          369 t
ORIGIN      
        1 gcgggcccga gccgagtggg gaggggcccg cgtccgtgcc gcacaacggc gagctcggcc
       61 ttgacaagtg ttctccatat tttccagaat atgcctgtga cctcagagaa gctcgattcc
      121 ctgtcgctcg aggcgctcta cgccgtggcc gacaagatgg ggctcgacct cccccccggg
      181 ctcgagcggc ttttcgtcgt cgaggagatc tccgaggcca tcgccgagga ttccgaggac
      241 cgccgcgccg cgcatggcga ggccgtccac gtcgaggaga agaaatactc ctcacccgag
      301 ctgggcgaga tcgacgccga tctggacagc tccggcgccg aggcgcccgt cctcgagacg
      361 cgctacaacg agacgatgat ccacgccctc gtccgcgatc cctcatgggc cttcgcgtat
      421 tgggacctca gcgacgccga ccagacggcc ctcttcggcg aggacggcgg ctcggccctc
      481 ttcctccgcg tcgtcgagct gggcggggag gacggccgcc gcgagtactt cgacatcccc
      541 gtctcgtccg aagaccacca gtggtacatc aacctgccaa ggcccggggt ccgcttcagg
      601 atcgacctct gcgtccggcc cggggacagc cccgtggcga ggatccgcgt cctcgcccgg
      661 tccaacgacg tcgagtcccc gaggcagagc ctcgagccgg accggcccct aagcgagcgc
      721 gtgatccgcc tcctggagct ctcgggcatc caggagctca acatcgagct cgcgagggac
      781 gagaacccgc agcgcatcct gccggcccgg agcgggcagg gcgagggcga ataaggaaca
      841 ggccgcatgc agaacggata cctcgccctg gtgctcaacg cgcacctgcc ctacgtgcgc
      901 cgccccgacc atccccgctt cctcgaggag cgctggctct tcgaggccct gtcggagacc
      961 tacctgcccc tgctccggct cttccgtcgc ctggacgagg actcggtgcc cttccacctg
     1021 accctcaaca tctcgcccac cctcgcggcg atgctcgggg acaaggtcct cggcgagcgc
     1081 tatctcgact acctcgacgc ccagctcagg ctggcctcgg ccgaggaggc caggctcggc
     1141 tccgaccccg ccttcgggcc actagcgagg ctctacgccg agctctaccg ggccgaccgc
     1201 gaggacttcg acatcaccta ttcgcgcaac atcctcggcg ccttcgacta cttctacaag
     1261 cgcggcagga tcgagctgat cacgacgggg gcgacccacg ccttccttcc cctcttccgc
     1321 gacagcgccg aggctgtgtc ggcccaggtc gagacggcga tcgtcgccca cagaaaggcc
     1381 ttcggcaagc accccgcggg cttctggctg ccccagctcg gctggttccc cggcgcggcc
     1441 gacatcctcc acgcctacaa cgtccagtac accgtcgtct ccacgagggg cgccctcctc
     1501 ggcgacccca cgccgcggaa cggctccttc gcccccgtcc agtgccccaa cggcctcacc
     1561 gtcttcatcc gcgactcggg ggcggtgaag gaggtctggt ccgagaccga gggctacccc
     1621 gccgatcccg tgtaccgcga cttctaccgc gacatcggct tcgaccttcc catcgaggac
     1681 ctgagcccct acaccgaggt ggggccgacg cgcatcttca ccggcttcaa gtactgggcc
     1741 gtgacgggca agaccgagca caaacgggtc tacgatcccg cggccgcctc ggcccgcgcc
     1801 gtcgagcacg ccgagcgctt cctcgaggcg aggagggccg cggccctggc cgcgggagcc
     1861 gacatggaca ggccgcccct catggtctgc ccctacgacg ccgagctctt cgggcactgg
     1921 tggttcgagg ggcctacctg gctcgaggcc ctcttccgca aggccgccgc cgagccttcg
     1981 ctccgtttcg tgaccctgga cgagtaccac aggacctatc ccgcgagcca ggtctcgatg
     2041 cccgagttct cctcctgggg cgacggaggc tacgccgagg tctggctcga cggctcgaac
     2101 gactggatct accgccacgt cttcaaggcc gccgagcgca tgtccgagct cgccgagcgc
     2161 ttccccgacg agtcgtggct tcgcgagcgc atcctgaacc aggccgctcg cgaggtgctc
     2221 ctctcgatgt gctcggattg ggccctgctc atgcgggccg gcaagtccgc gacgttcgcg
     2281 aggcgccagg tcgaggagcc gatcgccaac ttcggtcgca tctatgacat gctctgctcg
     2341 aacaccgtca gcaccgagtg gct
//
LOCUS       DASSQV010000002         9592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1492034, whole genome shotgun sequence.
ACCESSION   DASSQV010000002 DASSQV010000000
VERSION     DASSQV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 9592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9592
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492034"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VFL04_00015"
     CDS             <1..1325
                     /locus_tag="VFL04_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7838109.1"
                     /translation="GVEESLAALIRRPTVSWHDYGRADRAAFDGLAADLVGLFPLVHK
                     RLDRKEIGDWGLLYECRGSDPSLAPAILCAHYDVVPADEPEEWAHAPFSGDIAELFVW
                     GRGSQDVKCSLVCIMDAAERLLAQGFAPRRSVYFAFGADEEVGGPRGAAAIAGWLSAQ
                     GLRASFLLDEGGPVADGLFPLADRPLALIGIAEKGYIDVALIASGRGGHASMPPRRTA
                     TGDLARAIAAVEASPPPARLGYTLRQFLERLSPYVPFAQRLVFRNLWLLRGLVKAVFG
                     ASPATNALIRSTAAPTMLEASPKENVLADRARGTLNVRIIPGESSAQVLERLEGLVRR
                     TGVEVRPAHEGLTVESLPESPVDHEGYRRIEAALAAAFPEAAPVPFLFSASTDTKHYR
                     DVAEAIYRLTPLRQTTADLAGVHGRDERVSVANLRRCAIFYRALLASL"
     gene            complement(1438..2160)
                     /locus_tag="VFL04_00020"
     CDS             complement(1438..2160)
                     /locus_tag="VFL04_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7838110.1"
                     /translation="MLSVHDLHVSYGGIKALKGVSMEVNRGEIVTIIGANGAGKSTML
                     NTITGFLKPAKGEVVFNGQKLGRNPANIVKLGICHVPEGRLVFANLSVLDNLMLGAYL
                     RTDRKKIAGDLEKVFGLFPRLQERIKQQAGTLSGGEQQMLAMGRALMTDGELVLMDEP
                     SLGLAPLLVKTVFEIIVEIRRLGKTILLVEQNAFKALDIADRAYVLEQGHIVKEGPAC
                     DIQSDPAIRAAYLGSKSVAGGQ"
     gene            complement(2154..2936)
                     /locus_tag="VFL04_00025"
     CDS             complement(2154..2936)
                     /locus_tag="VFL04_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7838111.1"
                     /translation="MSAILELKGLTKQFGGLTAVNDVSFRVEEGGISGLIGPNGAGKT
                     TIFNLITGVYKVSSGAIEFQGKPIQNTEPYLIADRGITRTFQNIRLFKNMTVFENVLT
                     ACHASAHYGLMESIFRLPRYWRQEKELNAKASRLLEIMGLQDRMDITANNLPYGLQRR
                     LEITRALALDPKLLLLDEPAAGMNPDETEQLMRLIGQIREMFKLSVLVIEHHMDLIMG
                     VCEHIHVLSFGCTLADGTPAEIANDPKVVEAYLGSDEGKKSC"
     gene            complement(2933..3841)
                     /locus_tag="VFL04_00030"
     CDS             complement(2933..3841)
                     /locus_tag="VFL04_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7838112.1"
                     /translation="MLSSYLQQIIVLVGINMIAVLGLSILTGFTGLFSFGHAGFMAIG
                     AYVAAIFVARLHLPFWMAVTAGAAGAALVSIVIGALTLKLKGDYFCIATMGFGEAVRI
                     ILNNIDYVGGARGWESLPQLTSIWVVLILAVLMVVITWNLINSRHGRNMVAVREEELA
                     AQMAGINVFRYKMVALVVSAAMAGIAGALLGNYIQFLRPTMFNMVKSTDLTIMVIFGG
                     LGSISGTIVGTIILTVLPELFRNFAQWRLVIYGLAVIVIMVGRPQGVMGGFEMTPAGI
                     RKHFAARRARLALEAELRAAERGGAA"
     gene            complement(3853..4746)
                     /locus_tag="VFL04_00035"
     CDS             complement(3853..4746)
                     /locus_tag="VFL04_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7838113.1"
                     /translation="MTQFLQQLINGLSIGSIYALMAVGYSLVYSIMNFSNFAHGSVIM
                     LGAYFGFFSLTLLGLPFGIAFFIAACGAGLVAVTLERLAYKPLRDRRAPFLYFIITAM
                     GASILIDNGVIATIGPTPKTFQPELLPAYLQAGSIQIGSVGIGRVDIAMFVITAVCLV
                     ALILFIDFTKLGKAIQATAYNMKASALMGINPNFVIILVFAIGGALAGVAGVLYGVKY
                     QVFPQMSAITLKSFIAAVFGGLGSLPGALLGSLLLALIETFVSGYISTQLRDIFAFFI
                     LIAMLVIRPSGIMGKSAEDKA"
     gene            complement(4834..5988)
                     /locus_tag="VFL04_00040"
     CDS             complement(4834..5988)
                     /locus_tag="VFL04_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7838114.1"
                     /translation="MRALVCMVLVVALALMGCGGAQKSDTIKIGWLGPQTGDSALWGQ
                     AEYNTVKMMADDYNAAGGIDVGGKKYKVEIVAYDDKGDSTEAVNVAKRLTSQDKVVAV
                     VGPQGSGEAIPIAPIMNEAKVPCVASTATNPKVTVTENGTVNAYMFRACFTDPYQGKV
                     AAYAAYEKLGKRKAAIFMTIDDPYSTGLAQYFKESFESLGGKVVAQESFTSGEKDFRA
                     PLTKIKASNPDVIFMPNYYTDVALSAKQARDLGIKQLLLGGDGWPSDNLVSLAGNALE
                     GCFFINHLDMDGDAAKPMRDEYMAKFQKKAELNSYMVHDSLLMVIDAIQRAKSLKGED
                     IQKALTSTDIEGITGHIKIGPQNDPVGKTAWLIKIVGPDMKFQDKFAAAE"
     gene            complement(6340..6855)
                     /locus_tag="VFL04_00045"
     CDS             complement(6340..6855)
                     /locus_tag="VFL04_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838115.1"
                     /translation="MKGTRSALLALALALCLGPGRLWPQGLGPQTGARGTGPERAVLT
                     KGTFALRGLPFFGPNAIPALGGEYSLGAAAIPVWFTRESLVFSERWKRTTIGAHPAFL
                     LQPKEGQEAKGAVFALSLGAYSLFAQVPGDEAAGRLFVDALERRFAVFYANARNDSEL
                     SFPAFLDLGQP"
     gene            7006..7749
                     /locus_tag="VFL04_00050"
     CDS             7006..7749
                     /locus_tag="VFL04_00050"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET7838116.1"
                     /translation="MKETTSTMDEARRIARMGFPPGSLVAADLQSAGRGRLPERSWES
                     RPGSNLLFTLVLAPEEAGKPGLPLRAGLALCRTADILAVRMGLPLRSPAALKWPNDLL
                     FGAKKAAGVLCEAGPEGVFVGVGLNCNQKGFPLPLEGKATSLALEFGREVDRWLALEI
                     FLSTFRLLLSEEDWRQGVNERLWMRGKTVGFLVGLPGACDLVVGRLEGVDDMGCLVID
                     VPGGDGPRAYPAGEIVVDAPARQESRGPS"
     gene            7781..9352
                     /locus_tag="VFL04_00055"
     CDS             7781..9352
                     /locus_tag="VFL04_00055"
                     /inference="COORDINATES: protein motif:HMM:NF036553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HET7838117.1"
                     /translation="MKSTETTAKPGPVATASKEGLFQRILSIFAGIGDPDSEKKKLIK
                     GIGRDLQRSRYKFYRPKGQEALPNLAKFFYEVYRITAPAQVLLGNVSASGALRAFVIE
                     SFLTPDQLALSEKLTEEFVLARSKELQLKELQDEVKRELTNFTAVFDGETSRRIDSTY
                     NTLLAFINFVNFDYYFLLKKFDSAIPERNFSYHPKFEPINGDYVVEDLQDFLEVFLTI
                     DFETDWKRIFGAFKEYKKADVVQYEVWAKFLPAAMELRKSLVLDHIVRHLKRDPYWAS
                     TPRYPSERIVEPFLQKLNTHMETLVQRIVQERRNARIDEAAKQVFGTSVILRMKNYTE
                     KANVLFAKKMLGGYTQAPAMNYLKAYLMDYFKKDVREIADLLIIRGQWTTSLQSQQLS
                     DNYHAVLEVAEKIIQFDDSLADEGETGTKIRSSLAKAERDKEAVKYLRGILKDTNDKA
                     SAMVSRAAMNLIGVGRLLKVLIEDLDRPHHELLLNWKEVEAQSSRPLRDWLLVTYKQI
                     YHMVQLLQFFAKAEE"
BASE COUNT         1640 a         3147 c         3226 g         1579 t
ORIGIN      
        1 ccggggtcga ggagagcctc gcggccctca tacgccggcc caccgtctcc tggcacgact
       61 acggccgcgc cgaccgcgcc gccttcgatg gcctcgctgc cgacctcgtc gggctcttcc
      121 ccctcgtgca caaaaggctc gaccgaaagg agatcggaga ctggggcctg ctctacgagt
      181 gtaggggcag cgacccctca cttgcccctg ccatcctctg cgcccactac gacgtcgtcc
      241 ccgccgacga gcccgaggaa tgggcccatg cccccttctc cggcgacatc gccgagctct
      301 tcgtctgggg acgcggcagc caggacgtca agtgcagcct cgtctgcatc atggacgctg
      361 ccgagcgcct cctcgcgcag ggcttcgcgc cgaggcgcag cgtttacttc gccttcggcg
      421 ccgacgagga ggtggggggc ccccgcggcg cggcggcgat cgcgggctgg ctctcggccc
      481 agggcctgcg cgcctccttc ctcctcgacg agggaggccc cgtcgccgac ggcctcttcc
      541 ccctcgccga ccgccccctc gccctcatcg ggatcgcgga gaagggctac atcgacgtgg
      601 cgctcatcgc gtccggccgg ggcggccacg cctccatgcc gccgaggcgg accgccacgg
      661 gtgacctcgc cagggcgatc gccgcggtcg aggcctcgcc tcccccggcc aggctcggct
      721 ataccctgcg ccagttcctc gagcggctct cgccctacgt gcccttcgcc cagcgcctcg
      781 tgttccgaaa cctgtggctc ctccggggtc tggtcaaggc cgtcttcggc gcctcgccgg
      841 cgaccaacgc cctcatccgc agcacggcag cgcccacgat gctcgaggcc agccccaagg
      901 agaacgtgct cgccgacagg gcccgcggca ccctcaacgt gaggatcata ccgggggagt
      961 cctcggccca ggtcctcgag cgcctcgagg gcctcgtgag gcggacgggg gtcgaagtga
     1021 ggccggccca cgagggcctc acggttgaga gcctgcccga gtcgccggtg gaccacgagg
     1081 gctaccgccg catcgaggcg gccctcgccg cggccttccc cgaggcggcc ccggtgccct
     1141 tcctcttcag cgcgagcacg gacacgaagc actaccgcga cgtggccgag gcgatctacc
     1201 gcctcacccc cctccgccag accacggccg acctcgcggg ggtccacggc agggacgagc
     1261 gcgtctcggt ggccaacctc agacgctgcg ccatcttcta cagggccctc ctcgcctcgc
     1321 tctagacgaa aaaggaccgc ggggcgcgcc catcgggcgg ctccgcggtc caaaccttcg
     1381 ctgcttcggg ccccaggccg agggctgctc ttgcccccgg cagtcagccg tcgggtgcta
     1441 ctgccccccg gccaccgact tggagcccag gtaggctgcc cgtatcgccg ggtcgctctg
     1501 gatgtcgcag gcggggccct ccttgacgat gtggccctgc tcgaggacgt aggccctgtc
     1561 ggctatgtcg agggccttga aggcgttctg ctcgacgagg aggatcgtct tcccgagcct
     1621 gcggatctcg acgatgatct cgaagaccgt cttcacgagg aggggggcca ggccgaggga
     1681 gggctcgtcc atgaggacga gctcgccgtc ggtcatgagg gcccttccca tcgcgagcat
     1741 ctgctgctcg ccgcccgaga gggtgccggc ctgctgcttg atccgctcct ggagccgggg
     1801 gaagaggccg aagaccttct cgaggtcccc ggcgatcttc ttcctgtcgg tgcggaggta
     1861 ggcccccagc atgaggttgt cgaggaccga gaggttggcg aagaccagac ggccctcggg
     1921 gacatggcag atgccgagct tcacgatgtt ggcggggttg cggcccagct tctggccgtt
     1981 gaagaccacc tcgcccttcg ccggcttcag gaagccggtg atcgtgttca gcatggtcga
     2041 cttgccggcc ccgttcgcgc cgatgatggt cacgatctcg ccgcggttca cctccatcga
     2101 gacgcccttg agggccttga tgccgccgta cgagacgtgg aggtcgtgga cgctcagcat
     2161 gatttcttcc cttcgtcgga gccgaggtag gcctcgacga ccttggggtc gttggcgatc
     2221 tcggccggcg tcccgtccgc cagggtgcag ccgaaggaga ggacgtggat gtgctcgcag
     2281 acgcccatga tcaggtccat gtggtgctcg atcacgagga ccgagagctt gaacatctcg
     2341 cggatctggc cgatcaggcg catgagctgc tcggtctcgt cggggttcat gcccgcggcc
     2401 ggctcgtcca ggaggaggag cttggggtcg agggccaggg ccctcgtgat ctcaagccgc
     2461 ctctggaggc catagggcag gttgttcgcc gtgatgtcca tcctgtcctg gaggcccatg
     2521 atctcgagga gccggctcgc cttcgcgttg agctccttct cctgccgcca gtagcggggg
     2581 aggcggaaga tcgactccat gaggccgtag tgggcgctcg cgtggcaggc ggtgagcacg
     2641 ttctcgaaca ccgtcatgtt cttgaagagc ctgatgttct ggaaggtgcg cgtgatcccc
     2701 cggtcggcga tcaggtaggg ctcggtgttc tggatgggct tgccctggaa ctcgatggcg
     2761 cccgagctga ccttgtagac cccggtgatc aggttgaaga tggtcgtctt gccggcgccg
     2821 ttggggccga tgaggcccga gatgccgcct tcctcgaccc tgaagctcac gtcgttcacg
     2881 gccgtgaggc cgccgaactg cttggtgagg cccttgagct cgagtatcgc gctcatgccg
     2941 cgcccccccg ctcggccgcg cggagctcgg cctcgagggc cagcctcgcc ctgcgggcgg
     3001 cgaaatgctt gcggatgccc gccggcgtca tctcgaagcc gcccatgacg ccctggggcc
     3061 ggccgaccat gatgacgatg accgcgaggc cgtagatgac caggcgccac tgggcgaagt
     3121 tgcggaagag ctcgggcagg acggtcagga tgatggtgcc gacgatggtg cccgagatcg
     3181 accccaggcc gccgaagatc accatgatgg tcaggtcggt ggacttgacc atgttgaaca
     3241 tcgtgggcct gaggaactgg atgtagttgc cgaggagggc ccccgcgatg cccgccatgg
     3301 ccgcgctcac cacgagggcg accatcttgt agcggaacac gttgatgccc gccatctggg
     3361 cggccagctc ctcctcgcgt acggcgacca tgttgcgacc gtggcgcgag ttgatcaggt
     3421 tccaggtgat caccaccatg aggacggcga ggatgaggac gacccagatg ctcgtgagct
     3481 ggggcaggct ctcccagccg cgggccccgc ccacgtagtc gatgttgttg aggatgatcc
     3541 tgaccgcctc gccgaagccc atcgtggcga tgcagaagta gtcgcccttg agcttgaggg
     3601 tgagggcccc gatcacgatg gacacgaggg ccgcgccggc cgcgcccgcg gtgaccgcca
     3661 tccagaaggg caggtggagc cgcgccacga agatggcggc gacgtaggcc ccgatcgcca
     3721 tgaagcccgc gtgcccgaag gagaagaggc ccgtgaagcc cgtgaggatg gacaggccca
     3781 ggaccgcgat catgttgatg cccacgagta cgatgatctg ctgaagatag cttgacagca
     3841 tgctgtggcc ccctacgcct tgtcctcggc gctcttgccc atgatccccg aaggcctgat
     3901 cacgagcatc gcgatgagga tgaagaaggc gaagatgtcc cgaagctgcg tcgagatgta
     3961 gcccgagacg aaggtctcga tcagcgcgag caggagggac ccgaggaggg cgcccggcag
     4021 gctgcccagg ccgccgaaga cggcggcgat gaaggacttg agggtgatcg cgctcatctg
     4081 ggggaagacc tggtacttga ccccgtagag gacgccggcc acgccggcca gggccccgcc
     4141 gatcgcgaag acgaggatga tgacgaagtt cgggttgatg cccatgaggg cgcttgcttt
     4201 catgttgtag gccgtcgcct ggatcgcctt gcccagcttc gtgaagtcga tgaagaggat
     4261 gagggcgacg aggcagaccg cggtgatcac gaacatggcg atgtcgaccc tgccgatccc
     4321 cacgctgccg atctggatcg agcccgcctg gagatacgcg ggcaggagct cgggctggaa
     4381 ggtcttgggc gtcgggccga tcgtggcgat gacgccgttg tcgatcagga tggaggcgcc
     4441 catcgcggtg atgatgaagt agaggaaggg ggccctgcgg tcgcggaggg gcttgtaggc
     4501 caggcgctca agggtgaccg ccacgaggcc ggccccgcag gccgcgatga agaaggcgat
     4561 gccgaagggc aggccgagga gggtgagcga gaagaacccg aagtaggcgc cgagcatgat
     4621 gacgctgccg tgcgcgaagt tcgagaaatt catgatcgag tacacgaggg agtacccgac
     4681 cgccatgagg gcgtagatcg agcctatgga aaggccgttg atgagctgct ggaggaactg
     4741 ggtcattgct ccctttcctt gccaaaggaa aaagccggtc cgtctcggcc tctagtggcc
     4801 gcgaaaccgg accggcttcc ccctaggcgt accttattct gccgccgcga acttgtcctg
     4861 gaacttcatg tccgggccga cgatcttgat gagccacgcg gtcttgccga ccgggtcgtt
     4921 ctggggcccg atcttgatgt ggccggtgat gccctcgatg tcggtgctgg tgagggcctt
     4981 ctggatgtcc tcgcccttga gcgacttcgc cctctggatg gcgtcgatga ccatgaggag
     5041 ggagtcgtgg accatgtagg agttgagctc ggccttcttc tggaacttgg ccatgtactc
     5101 gtcgcgcatc ggcttggcgg cgtcgccgtc catgtcgagg tggttgatga agaagcagcc
     5161 ctcgagggcg ttgccggcga gggagacgag gttgtccgaa ggccagccgt cgccgccgag
     5221 gaggagctgc ttgatgccga ggtcgcgggc ctgcttcgcg gagagggcga cgtcggtgta
     5281 gtagttgggc atgaagatca cgtcggggtt ggaagccttg atcttggtga ggggggcgcg
     5341 gaagtccttc tcgcctgagg tgaagctctc ctgggccacg accttcccgc cgaggctctc
     5401 gaagctctcc ttgaagtact gggcgagacc ggtggagtag ggatcgtcga tggtcatgaa
     5461 gatcgcggcc ttgcgcttgc cgagcttctc atacgcggcg taggcggcca ccttgccctg
     5521 gtaggggtcg gtgaagcagg cgcggaacat gtacgcgttg accgtcccgt tctcggtgac
     5581 cgtgaccttg gggttcgtcg cggtgctggc cacgcagggg accttcgcct cgttcatgat
     5641 gggcgctatc gggatggcct cgcctgagcc ctggggcccg acgacggcga cgaccttgtc
     5701 ctggctcgtg aggcgcttcg cgacgttgac ggcctcggtg gagtcgccct tgtcgtcgta
     5761 ggcgacgatc tcgaccttgt acttcttgcc gcccacatcg atgccgccgg cggcgttgta
     5821 gtcgtcggcc atcatcttca ccgtgttgta ctcggcctgg ccccacaggg cgctgtcgcc
     5881 ggtctgagga ccgagccagc cgatcttgat cgtgtcggat ttctgcgcgc cgccgcagcc
     5941 catgagggcc agagcaacga cgagtaccat acaaacgagc gctcgcatga aaagcctcct
     6001 tcacaaggga acgtaaggat ttgcgccagt atacaggagg gttcgcggct ccacaataaa
     6061 aaagcgcccc tgcccgggct atttccgcgg ccgcggggcc tcgtcgcgct ccatcacagg
     6121 cgggaaatcc cgcgcatgat tatgcgcaga atttagggcc gcccgcgcca atcggcaagc
     6181 gggggcggaa aacgcctgca tattcatgcg ccccgctttg cctgcgccgg gcgggctgct
     6241 cggggcgctc aggatttcgg gaaagaatcc caggggatcg ccggcaaggc cccggggccc
     6301 gtgcccgagg cctggccctg ggccacgcta gggcgtggct cagggctggc cgaggtcgag
     6361 gaaggcgggg aagctgagct ccgagtcgtt cctcgcgttc gcgtagaaga ccgcgaagcg
     6421 ccgctccagg gcatcgacga agagccgccc cgccgcctcg tccccgggga cctgggcgaa
     6481 gagggagtag gcgccgaggc tgagggcgaa gacggccccc ttggcctcct ggccctcctt
     6541 cggctggagc agaaaggccg ggtgcgcgcc tatcgtcgtc ctcttccagc gctccgagaa
     6601 gacgaggctc tcgcgggtga accagacggg gatcgccgcc gcgccgaggg aatactcccc
     6661 gccgagggcg ggtatcgcgt tggggccgaa gaagggcaga cccctgaggg cgaaggttcc
     6721 cttggtgagg accgcgcgct ccggtcccgt gcccctggcc ccggtctggg ggccgaggcc
     6781 ctggggccag agccggccgg gtccgaggca gagggccaag gcgagggcga ggagggcgct
     6841 gcgggttccc ttcatgtcgg tgcggataag tatacacccc cgtccgcaac ggggacaatc
     6901 gcggccccct ccccggtttg aactgggggg cccgctcgac tagaattgaa gcctatggag
     6961 aagctcgaaa tcccgaatcc cttccccagc gcaggatgtt tcttcatgaa agaaaccacc
     7021 tcgaccatgg acgaggcgag gaggatcgcc aggatgggct ttccccccgg cagcctcgtc
     7081 gcggccgacc tgcagagcgc cgggagggga cgtctccccg agcggtcctg ggaatcgagg
     7141 ccgggctcga acctcctgtt caccctcgtc ctggcccccg aagaggccgg gaagccgggc
     7201 cttcccctca gggcgggcct ggccctctgc cgcaccgccg acatcctcgc ggtgaggatg
     7261 ggccttcccc tgcgcagccc ggcggcgctg aaatggccga acgacctgct tttcggcgcg
     7321 aaaaaggccg ccggggtcct ctgcgaggca gggcccgagg gggtcttcgt cggggtgggc
     7381 ctcaactgca accagaaggg cttccccctc cccctcgagg gcaaggcgac gagcctggcc
     7441 ctcgagtttg ggcgcgaggt ggaccgctgg ctcgccctcg agatcttcct ctcgaccttc
     7501 aggctgcttc tgtccgagga ggactggagg cagggcgtga acgaaaggct gtggatgcgc
     7561 ggcaagaccg tcggcttcct cgtgggcctg ccgggggcct gcgacctcgt ggtcgggagg
     7621 ctcgagggcg tcgacgacat gggctgcctc gtgatcgacg tcccgggcgg ggacggcccc
     7681 agggcctatc cggccgggga gatcgtcgtc gacgcgcccg cgcggcaaga aagccgcggc
     7741 ccgagttgac cgctacgacc cattcttata aggtgagtcc atgaagagca ctgagacgac
     7801 ggcgaagcca gggccagtcg ccacggcgtc gaaagagggc ctgttccagc ggatcctttc
     7861 gatcttcgcc gggatcggcg accccgacag cgaaaaaaag aaactcatca aggggatcgg
     7921 ccgcgacctc cagcgctcac gctacaagtt ctaccgcccc aagggccagg aggcgctgcc
     7981 gaacctggcc aagttcttct acgaggtcta caggatcacc gcccccgccc aggtcctcct
     8041 cggcaacgtc tcggcctcgg gggccctccg ggccttcgtg atcgagagct tcctgacccc
     8101 cgatcagctc gcgctctccg agaagctcac cgaggagttc gtgctcgccc ggtccaagga
     8161 gctccagctc aaggagctcc aggatgaggt caagcgcgag ctgaccaact tcaccgccgt
     8221 cttcgacggg gagacgagcc gcaggatcga ctcgacctac aacaccctcc tcgccttcat
     8281 taacttcgtc aacttcgact actacttcct cctcaagaag ttcgactcgg ccatccctga
     8341 gcgcaacttc tcctaccacc ccaagttcga gccgatcaac ggcgactatg tcgtcgagga
     8401 cctccaggac ttcctcgagg tcttcctcac catcgacttc gagaccgact ggaaacggat
     8461 cttcggggcc ttcaaggagt acaagaaggc cgacgtcgtc cagtacgagg tctgggccaa
     8521 gttcctgccc gcggcgatgg agctgcgcaa gagcctggtc ctcgaccaca tcgtccgcca
     8581 cctcaagcgc gacccctact gggcgagcac gccccgctac ccctcggagc gcatcgtcga
     8641 gcccttcctg cagaagctca acacccacat ggagaccctg gtccagagga tcgtgcagga
     8701 gcgcaggaac gccaggatcg acgaggccgc caagcaggtc ttcggcacct cggtgatcct
     8761 caggatgaag aactacaccg agaaggccaa cgtcctcttc gcgaagaaga tgctgggcgg
     8821 ctacacccag gccccggcca tgaactacct caaggcctac ctcatggact acttcaagaa
     8881 ggacgtccgc gagatcgccg acctcctcat catccgcggc cagtggacga cgagcctcca
     8941 gtcccagcag ctctccgaca actaccacgc cgtcctcgag gtggccgaga agatcatcca
     9001 gttcgacgac tccctcgccg acgagggcga gaccggcacg aagatccgct cctccctggc
     9061 gaaggccgag cgggacaagg aggccgtgaa gtacctccgc ggcatcctca aggacaccaa
     9121 cgacaaggcc tcggccatgg tctcgagggc ggcgatgaac ctcatcggcg tgggccgcct
     9181 cctcaaggtc ctgatcgagg acctcgaccg gccccaccac gagctgctgc tcaactggaa
     9241 ggaggtcgag gcccagagct cccgccccct gcgcgactgg ctcctcgtca cctacaagca
     9301 gatctaccac atggtccagc tgctccagtt cttcgcgaag gccgaggagt aaagagaaag
     9361 gcacgccacg gaggcacgga gtctggccac ggagatcacg gaggagaaag agagaggtca
     9421 ggaggaagaa cctcgactga tctcaccgcg gaggacgcgg agagcgcaga ggggaagaag
     9481 gatttcacca cggaaactta gtccttgcca ccgccaactc gtcttattca acctcgagga
     9541 cacatcccca ggcccgcgag ggagaagaca tgggcttccc ggcaatcttg tc
//
LOCUS       DASSQV010000003         4118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1616809, whole genome shotgun sequence.
ACCESSION   DASSQV010000003 DASSQV010000000
VERSION     DASSQV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616809"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFL04_00060"
     CDS             complement(<1..529)
                     /locus_tag="VFL04_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838118.1"
                     /translation="MRLGAGEGSAGFAARDGGALLRSNLEAIGSRFPELAAILAETPA
                     DELETEATLSGEPTARPAGGAWLHSTRSPRDEAKRLAAACLSAGEDCALLLGFGLGYQ
                     AEACLEAGATMVIVVEAEPGMLKAALCARELGVLLADERLGFIVGGEPEAVIGALELS
                     GARKAGILALKGAERY"
     gene            complement(526..2418)
                     /locus_tag="VFL04_00065"
     CDS             complement(526..2418)
                     /locus_tag="VFL04_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET7838119.1"
                     /translation="MSRRAFRYWSVIGLLGLFALLILIKYAGLAIGSPAGAAAGAVEV
                     ERGPIMDRSGRVLALDSPLYNVAVWRPETVVETFPAEAKRLATILGMDETELQGRWKT
                     EKADFFYLRKRVQPQIARAIQEGRAAGSFSGIVVEKVSGRLYPEKRLASHLVGFVGDA
                     NKGLAGVEARYDEDLSAGAKGQAEARGKAEAGAPVRGDSVSLSIDSDIQFSLEEVARK
                     AMTDTGAQAVMLVAADARTGEILAYVALPDFDPNEYLLAPAEAWYDWPSVYAYEPGSV
                     FKVFSMASVLDLGGATTRSTFVCDGAYHRTAPSGEPITIKCLAVHGTVDIEHILEYSC
                     NAGAGYASDTVQALDFYERLRGFGFGSRLGLALAGESPGQLRSPETWSLRSKPTIAMG
                     QEVLVTALQMTAAATAIANGGVVLKPIIVKRVAGPDGTTVYENAPQAVRRAVSAETAS
                     AILASMETVSSESGTGRRAKVKDVRMAVKTGTAQMIDPQTRRYSEKDYIASTLAIFPA
                     DEPRVVLYLAIVKPTGESYFGGRIAAPVIKDAAEAVLSLTDLPRGASPSVLHPGVVTI
                     PKPEAVSVGQVMPDLTGTPKRLLLPLLVRKDILVHISGEGYVVGQKPAPGSPVAPGTE
                     IFLELK"
     gene            complement(2415..2933)
                     /gene="lepB"
                     /locus_tag="VFL04_00070"
     CDS             complement(2415..2933)
                     /gene="lepB"
                     /locus_tag="VFL04_00070"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7838120.1"
                     /translation="MARTILRPGRAVIAVILSVLFAKAFILDFAVVDGRSMLPSLEPG
                     SVVLVLRCAYGLSRPFGGGYLATWASPRRGDLVAGSNPRNGEAIVKRAAAVGPATLVV
                     EAGRLLGPGIDVELRGQAEAGLGPNPKVPRGSLFLLGDNPGESIDSRDYGSVPIESLS
                     GKVLSFRSWAPQ"
     gene            2992..>4118
                     /locus_tag="VFL04_00075"
     CDS             2992..>4118
                     /locus_tag="VFL04_00075"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7838121.1"
                     /translation="MESLIARQKIARRRPLAIAATEKAGFSGQAGPKRRDPRRKLPVD
                     FFVLEGGARGPILGPAAPKGGLRLLRGLIPTAKARGQGPRPKLGPASSSRAITPFAAG
                     RSGGILHLLRAIPATVYGSLAARVASRVPRAPRAPASLSTKLLLLGLAAALLAAGLAG
                     ALALGRSLAFPLPAAGLLPATVPIDEYLLDYVSPELSADSSDPGELAPAPPPTLEVSS
                     YTVKNGDSLAKVARRFGLNVDTLISMNGTAAVRAFKTGTELKVPNMNGIVHRVSAGQS
                     IGSIAKLYGSSVTALVDANDLGTTVIRAGQVVFIPGARLPDAEIRKVYGDKVAWPARG
                     PLSSYFGYRADPFTGVRSYHAGLDIVVAPGTPVRAAMDGVVA"
BASE COUNT          604 a         1469 c         1440 g          605 t
ORIGIN      
        1 ggtagcgctc tgcccccttg agggcgagga tgccggcctt ccttgccccg gagagctcga
       61 gggcgccgat caccgcctcg ggctcaccgc cgacgatgaa gccgagcctt tcgtcggcga
      121 ggaggacgcc gagctccctc gcacagaggg cagccttgag catccccggc tcggcctcga
      181 ccacaatgac catcgtggcc cccgcctcga gacaggcctc ggcctggtag ccgaggccaa
      241 agcccagaag aagcgcgcag tcctcgccgg cggaaaggca ggctgcggcg agcctcttgg
      301 cctcgtcccg gggcgagcgg gtcgagtgga gccaggcccc tcccgccggc ctggccgtcg
      361 gctcgcccga gagcgtcgcc tcggtctcga gctcatcggc aggcgtctcg gccaggatcg
      421 ccgcgagctc ggggaagcgc gagccgatgg cctcgaggtt ggagcgcagc agggctcccc
      481 catcccgggc cgcgaagccc gccgatccct cccctgcccc gagcctcatt tgagctccag
      541 gaagatctcg gttccagggg ccacggggct tccgggggcc ggtttctggc ccaccacata
      601 gccctcgccg gagatgtgga ccaggatgtc cttgcgcacg aggaggggga ggaggaggcg
      661 tttcggcgtc cccgtgaggt cgggcatgac ctggcccacg ctgaccgcct cgggcttggg
      721 gatggtgacc acgcccggat ggaggacgct cgggctggcc cctcggggga ggtcggtcag
      781 gctgaggacg gcctcggccg cgtcctttat gacgggggcc gcgatgcgtc cgccgaagta
      841 ggactccccg gtgggtttca cgatggcgag gtagaggacg acccggggct cgtcggcggg
      901 gaagatcgcc agggtcgagg cgatgtagtc cttctcagag tagcgtctgg tctgggggtc
      961 gatcatctgg gccgtgcccg tcttgaccgc catccgcacg tccttgacct tggccctcct
     1021 gccggtgccg ctctcgctgg acacggtctc catcgaggcc aggatcgcgc tcgccgtctc
     1081 ggcgctcacg gccctccgca cggcctgggg cgcgttctcg tagaccgtgg tcccgtccgg
     1141 cccggccacc cgctttacga tgatgggctt gagcacgacc ccgccgttgg ctatcgccgt
     1201 cgccgcggcc gtcatctgga gggcggtcac gaggacctcc tggcccatcg cgatggtcgg
     1261 cttggacctg agcgaccagg tctcggggga gcgcagctgg ccggggctct cgccggcgag
     1321 ggccaggccg agccgggagc cgaagccgaa gccgcgcagc cgctcgtaga agtccagggc
     1381 ctggaccgtg tccgaggcgt agcccgcgcc ggcgttgcag gagtactcga ggatgtgctc
     1441 gatgtcgacg gtgccgtgga cggccaggca cttgatggtg atcggctcgc ccgagggcgc
     1501 cgtcctgtgg taggccccgt cgcagacgaa ggtcgagcgc gtggtggccc cgccgaggtc
     1561 gaggaccgag gccatggaga agaccttgaa caccgagccg ggctcgtagg cgtagacgct
     1621 gggccagtcg taccaggcct cggccggggc gaggaggtac tcgttggggt cgaagtcggg
     1681 cagggcgacg taggcgagga tctcgcccgt gcgcgcgtcg gcggccacga gcatgaccgc
     1741 ctgggccccc gtgtcagtca tggccttgcg cgccacctcc tcgagcgaga actggatgtc
     1801 ggagtcgatg ctgaggctga cgctgtcgcc gcggaccggg gccccggcct cggcctttcc
     1861 ccttgcctcg gcctgaccct tcgccccggc cgagaggtcc tcgtcgtagc gggcctcgac
     1921 ccccgcgagg cccttgttgg cgtcgccgac aaagcccacg aggtgggagg cgaggcgctt
     1981 ctccgggtag agcctgcccg agaccttctc gacgacgatg ccggagaagg agccagccgc
     2041 cctgccctcc tggatcgcgc gggcgatctg gggctggacc cgcttcctaa gatagaagaa
     2101 atcggccttt tcggtcttcc agcggccctg gagctcggtc tcgtccatgc cgaggatggt
     2161 cgccagacgt ttggcctcgg cggggaaggt ctcgaccacg gtctcgggcc tccagaccgc
     2221 gacgttgtag aggggggaat cgagggcgag gacgcggccg ctccggtcca tgatcggtcc
     2281 ccgctccacc tcgacggctc cggccgcagc gcccgcgggc gagccgatgg ccaggcccgc
     2341 gtattttatg aggatgagga gggcgaaaag cccgaggagg ccgatgaccg accagtagcg
     2401 gaaagccctt cggctcattg cggcgcccat gagcggaagg ataacacctt accgctgagg
     2461 ctctcaatcg gcacgcttcc gtagtcccgg gagtcgatgg actccccggg gttgtcgccg
     2521 aggaggaaga ggctgccgcg cggcaccttc gggttcggcc ccaggccagc ctcggcctgg
     2581 cccctgagct cgacgtcgat gccggggccg aggagccgcc ccgcctcgac cacgagggtc
     2641 gccggaccga cggccgccgc gcgtttgacg atggcctcgc cgttcctcgg gttggagccg
     2701 gccacgaggt cgccccgacg cgggctggcc caggtggcca gatagcctcc gccgaagggc
     2761 cgggagaggc cgtaggcgca gcgcaggacc aggaccaccg atcccggctc gagtgagggc
     2821 agcatggagc ggccgtcgac gacggcgaag tcgaggatga aggccttggc gaagaggacg
     2881 gacaggatga cggcgatgac ggcccgtccc ggccggagga tcgttcgggc catgagtgag
     2941 ctccgactat aatgccccga caccgaattg tcgataagac tgtaagccgc catggaaagc
     3001 ctgatagcac gccagaagat cgcacgccgc cgtcccctcg ccatcgccgc cacggagaag
     3061 gccggcttct ccggccaagc gggccccaaa cgcagggatc ccaggcggaa actccccgtc
     3121 gacttcttcg tccttgaggg aggggcccgt ggccccatcc tcggccctgc agcgcccaag
     3181 ggcgggctcc gcctcctccg cggcctcatc ccgactgcca aagcccgcgg ccagggcccg
     3241 aggccaaagc ttggcccggc ctccagctcc cgcgccatca cccccttcgc ggcgggaagg
     3301 tccgggggca tcctccatct gctccgcgcc atccccgcga ccgtctatgg cagcctggcc
     3361 gcccgcgtgg cctccagggt tcccagggct cccagggcgc cggccagcct gagcacaaag
     3421 ctcctcctcc tcggcctcgc cgccgccctc cttgcggcag gcctggccgg agccctcgcc
     3481 cttgggcggt ccctggcctt ccccctcccc gccgcgggcc tcctgccagc gacggtgccc
     3541 atcgacgaat acctcctcga ctatgtctcg cccgagctct cggccgactc ctccgacccg
     3601 ggcgagcttg cccccgcccc accccccacc ctcgaggtct caagctacac cgtcaagaac
     3661 ggcgacagcc tggccaaggt ggcgcggcgc ttcggcctca acgtcgacac cctcatctcg
     3721 atgaacggga ccgcggccgt ccgcgccttc aagacgggga cggagctcaa ggtgcccaac
     3781 atgaacggca tcgtgcaccg ggtgagcgcc ggccagagca tcggctcgat cgcgaagctc
     3841 tacggcagca gcgtcaccgc ccttgtggac gcgaacgacc tgggcacgac ggtgatcagg
     3901 gccggccagg tggtcttcat ccccggcgcc aggcttccgg acgcggagat caggaaggtc
     3961 tacggggaca aggtggcatg gccggcccgc ggcccgcttt cctcctattt cggctaccgg
     4021 gccgacccct tcaccggcgt ccgctcctac cacgccggcc tggacatcgt cgtggccccg
     4081 gggaccccgg tgcgcgcggc gatggacggg gtggtcgc
//
LOCUS       DASSQV010000004         4731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_871569, whole genome shotgun sequence.
ACCESSION   DASSQV010000004 DASSQV010000000
VERSION     DASSQV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871569"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2478)
                     /gene="fdhF"
                     /locus_tag="VFL04_00080"
     CDS             complement(<1..2478)
                     /gene="fdhF"
                     /locus_tag="VFL04_00080"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HET7838122.1"
                     /translation="MASEPVVDISIDGKTVKAREGEPLIVAAREAGFPIPSLCWHRKL
                     SPTGACRLCVVKIEGSRGLVTSCSTPVKAGMVVTAFDEELEEARRYILDYLLAEVDCG
                     TDGSFRDEFAELVERYGLSEPSARRFPRLGPGAAEQTVDSSSPALYFDPRRCIKCFRC
                     IKACAEVQGKGVLQMAERGLGSVVLAGSGIWGASECDGCGECVQLCPTGAIVEKPHRR
                     EIDLARIERRVRTTCPYCGVGCQIELLIQDGRIIRVEGVEGQSPNDGRLCVKGRFGYD
                     FVHSPDRLRSPLIRDGGGFREATWDEALTAAAEGLSRVKKRHGPHALAGYSSAKCTNE
                     ENYLFQKLVRVAFGTNNVDYCTRLCHASTVTAMLKAIGDAAGSNSIEDFERADCLIVI
                     GDNLVETHPVTATYVKRAKARGGHIVVIDPKETPLARYADLWLRPRLGTDVALLNGMT
                     RVAIEQGWVDREFIEARVQGGMGAFVELERLCSAYTPARVLEICGVGERDLVEATRLY
                     ATAPRAMIATGMGMSQQTTGTHGVFSLMNLMLVTGKIGKEGCGIDPPRGQNNVQGATD
                     VGASPIYYPGYIPASDEANRRTLARLWGIGADELPGKRGLTTIEIAKAAHEGQIRGML
                     IMGENPIMTDPNSAHVEEALRSLEFLVVQDIFMTRTAELAHVVLPAASFAEKDGTFTN
                     SDRRVLRVRRAVASPGGASEDLWILQEIAARMGRPIGSYTGAEAVFDELAKAAPILGG
                     IDYGRLEARGIQWPCPSPGHPGTPTLFLERFSTADGLARFNPVEYEEQSELADAQWPF
                     ILNSGRILYQYHSSTMSRRCPPLVEFAN"
     gene            complement(2493..3734)
                     /locus_tag="VFL04_00085"
     CDS             complement(2493..3734)
                     /locus_tag="VFL04_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HET7838123.1"
                     /translation="MAEERRIALSFLGRVDPGSIDDYIAAGGYAGLKAALAMDPGSVV
                     AELSRAGLRGRGGAGFPTGRKELSTAEACGECDCFVVCNADEGEPGTFKDRIIMEGEP
                     QLLIEGMIISAYAIGAGYGYVYIRGEYARSIELVGKAIEEAKARGFLGRGILGSAFSF
                     DIELRRGAGSYLCGEELTLIESLEGKRGYPRIKPPFPAEVGYRGTPTLVNNVETFANL
                     PFIVEQGADAYLALGTPSSPGTKIFCISGDVVRPGFAELEFGVSLRSIIDDLAGGVSE
                     GGPALAVLLGGAAGTFVAGEDLDIPMDFDSLRKQGLSLGSGAVIVLGAHRSLAATISS
                     IMDFFAHESCGKCIPCRVGTTRLAASAARLGSIPKGERKALLLSMVDEARLMASTSLC
                     PLGQSPILPLESVARRLADSL"
     gene            complement(3727..4164)
                     /locus_tag="VFL04_00090"
     CDS             complement(3727..4164)
                     /locus_tag="VFL04_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET7838124.1"
                     /translation="MESRDRLLSALHEIQDRSPQNYVSPDELDGLSHRFGLTMAELRG
                     VSSYYSMISLEPRGRHIVRVCVSPVCRMLGALDLLGLLKAELGIEVGETDPEGLFTLE
                     ETQCLGHCAGAPAMMVGARVYEGLDVEGVRGIIAAYRKARDHG"
     gene            4286..>4731
                     /locus_tag="VFL04_00095"
     CDS             4286..>4731
                     /locus_tag="VFL04_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838125.1"
                     /translation="MKMTIGKKLAFGFGAILALVVAYGVTMIVELRGLERSSQTMTTR
                     SDNELKIAVAARLGPLIYQTIADAVINRGDLQAIEKRWTEVKASAIAELESIRPALAT
                     ETEREQLAEAERRLAEVATKFEGGTLVQLDRGARADLELVSRIDGD"
BASE COUNT          747 a         1600 c         1605 g          779 t
ORIGIN      
        1 gttggcgaac tcgaccaggg gagggcatcg cctcgacatc gtcgaggaat ggtactggta
       61 gaggatccgg ccactgttga ggatgaaggg ccactgggca tcggccagct cgctctgctc
      121 ctcgtactcg acgggattga agcgggccag gccgtcggcc gtgctgaagc gctccaggaa
      181 gagggtcggc gtgcccggat ggcccgggct cgggcagggc cactggatcc ccctggcctc
      241 gagccgcccg tagtcgatcc cgccgaggat cggggctgcc ttggccagct cgtcgaagac
      301 cgcctcggcc ccggtatagg agccgatggg cctgcccatc ctcgcggcga tctcctgaag
      361 gatccacagg tcctcgctgg ccccgccggg gctcgccaca gccctcctca ccctgagcac
      421 ccgtctgtcg gaattggtga aggtaccatc cttctccgcg aaggaggcgg cgggcaggac
      481 cacgtgggcg agctcggccg tcctggtcat gaagatgtcc tggacgacga ggaactcgag
      541 gctccgcagg gcctcctcga cgtgggccga gttcgggtcg gtcatgatgg ggttctcgcc
      601 catgatcagc atgcccctga tctgcccctc gtgggccgcc ttggcgatct cgatggtcgt
      661 gagcccgcgc ttccccggca gctcgtccgc tccgatgccc cagagcctcg cgagggtcct
      721 ccggttcgcc tcgtccgagg cggggatgta gcctgggtag tagatcggcg aggcgcccac
      781 gtctgtcgcg ccctggacgt tgttctggcc gcggggcggg tcgatcccgc agccctcctt
      841 gccgatcttg cctgtgacga gcatgaggtt catgaggctg aacacgccgt gggtccccgt
      901 ggtctgctgg ctcatgccca tgccggtcgc gatcatggcc cgcggggccg tggcatacag
      961 ccgcgtggcc tcgacgaggt cccgctcgcc caccccgcag atctcaagga cccgagcggg
     1021 cgtgtaggcc gagcacaggc gctcgagctc gacgaaggcc cccatgccgc cctggaccct
     1081 cgcctcgatg aattccctgt ccacccagcc ctgctcgatc gcgacgcgcg tcatgccgtt
     1141 gaggagggcg acgtcggtgc cgagccttgg cctgagccag agatcggcgt agcgggcaag
     1201 gggggtctcc ttgggatcga tcacaacgat gtggccgccc cgagccttcg cccgcttcac
     1261 gtaggtcgcc gtgacggggt gggtctcgac gaggttgtcg ccgatcacga tgaggcagtc
     1321 tgcccgctcg aaatcctcga tcgagttgct gccggccgcg tccccgatcg ccttcagcat
     1381 cgcggtcacg gtcgacgcgt ggcagagccg ggtgcagtag tccacgttgt tcgtgccgaa
     1441 ggcgacgcgc accagcttct ggaagaggta gttctcctcg ttcgtgcatt tcgccgagga
     1501 gtagccggcc agggcatggg gtccgtgccg cttcttgacc ctggagaggc cctcggcggc
     1561 cgcggtgagg gcctcgtccc aggtggcttc cctgaagccc ccgccatccc ttatgagggg
     1621 gcttcggagg cggtctgggc tgtggacgaa gtcgtagccg aagcggccct tcacgcagag
     1681 cctgccgtcg ttcggggact ggccctcgac cccctcgacc ctgatgatcc tcccgtcctg
     1741 gatgaggagc tcgatctggc agcccacccc gcagtagggg caggtcgtgc ggacgcggcg
     1801 ctcgatcctc gcgaggtcga tctccctcct gtggggcttc tccacgatgg cccccgtggg
     1861 gcagagctgg acgcactccc cgcagccgtc gcactccgag gccccccaga tgcccgagcc
     1921 cgcgagcacc accgagccga ggccccgctc ggccatctgc agcactccct tgccctggac
     1981 ctcggcgcag gccttgatgc agcggaagca cttgatgcag cgcctggggt cgaagtacag
     2041 ggcgggcgag gacgagtcga cggtctgctc ggccgctccg ggcccgagcc tcgggaatcg
     2101 cctcgccgag ggctcggaga ggccgtagcg ctccacgagc tcggcgaatt cgtcgcggaa
     2161 gctcccgtcg gtcccgcagt ccacctcggc gaggaggtaa tcgaggatgt agcggcgcgc
     2221 ctcctcgagc tcctcgtcaa aggcggtgac gaccatgccc gccttgaccg gggtggagca
     2281 ggaggtgacg aggccgcgag aaccctcgat cttcaccacg cagagcctgc aggcccccgt
     2341 cggactcagt ttgcggtgcc agcaaaggct cggtatgggg aatcccgcct ccctggccgc
     2401 gacgatcagc ggctcgccct cgcgggcctt gaccgtcttg ccatcgatgc tgatgtcgac
     2461 cacaggctca ctcgccatga ctgctcctgc gctcagaggg aatcagcgag gcgtctcgcc
     2521 acgctttcga gggggaggat gggggactgt ccgagggggc agagcgaggt cgaggccatg
     2581 agcctcgcct cgtccaccat cgaaaggagg agggccttcc tttccccctt ggggatcgag
     2641 ccgagccgag ccgccgacgc cgcgagccgc gtcgtgccca ccctgcaggg tatgcacttt
     2701 ccgcaggact cgtgcgcgaa gaagtccatg atcgaggaga tcgtcgcggc caaggaacga
     2761 tgggcgccga ggacgatgac ggctccggag ccgaggctca ggccctgctt ccttaggctg
     2821 tcgaaatcca tcgggatgtc caggtcctcg ccagccacaa aggtcccggc ggctcccccg
     2881 aggaggacgg cgagggctgg cccgccctcc gagaccccgc ccgcgaggtc gtcgatgatg
     2941 ctgcggaggc tgacgccgaa ctcgagctcg gcgaaacccg gccgcacgac gtcgccggag
     3001 atgcagaaga tcttcgtgcc gggcgaggat ggggtcccga gggcgaggta ggcatcggcg
     3061 ccctgctcga cgatgaaggg caggttggcg aaggtctcga cgttgttgac gagggtgggc
     3121 gtgccccggt accccacctc ggcggggaag ggcggtttga tccgaggata gccccgcttg
     3181 ccctcgaggg actcgatgag ggtgagctcc tccccgcaga gatatgatcc ggcgccccgg
     3241 cgcagctcga tgtcgaagga gaaggcgctg ccgaggatgc cccttccgag gaagccgcgg
     3301 gccttggcct cctcgatggc cttgcctacg agctcgatgg agcgggcgta ctccccgcgg
     3361 atatagacgt agccatagcc agccccgatg gcgtaggccg agatgatcat gccctcgatc
     3421 aggagctggg gctccccctc catgatgatc ctgtccttga aggtccccgg ttcgccctcg
     3481 tcggcgttgc agacgacaaa gcagtcgcat tccccgcagg cctcggcggt gctgagctcc
     3541 ttcctccctg tcgggaagcc cgctccaccc cttcccctga gcccggcgcg ggacagctcg
     3601 gccacgactg agccgggatc catggcgagg gcggccttca gcccggcata gcctccggcc
     3661 gcgatgtagt cgtcgatgct ccccgggtcg accctgccga ggaaggagag ggcgatcctt
     3721 ctttcctcag ccatggtccc gagccttgcg ataagcggcg atgatccccc ggacgccctc
     3781 cacgtcgagc ccctcataga cccgggcgcc gaccatcatc gcgggggctc ccgcgcagtg
     3841 ccccaggcat tgggtctcct cgagggtgaa aagaccttca gggtcggtct cgccgacttc
     3901 gatgccaagc tcggctttga ggaggcccag gaggtcgagg gcgccgagca tgcggcagac
     3961 gggggagacg cagaccctga cgatgtgcct ccctcggggc tcgaggctga tcatgctgta
     4021 gtagctcgag acgccccgga gctcggccat cgtcagcccg aagcggtggg agaggccgtc
     4081 gagctcgtcg ggcgagacat agttctgcgg ggatctgtcc tggatctcat ggagtgcgga
     4141 aaggaggcgg tctcttgact ccatgccgga agtatagccc cccctgggcc tgtggtcaaa
     4201 aggacaattt cgattgcctt cctcttgtgc tgcccggcca aacccgacca tactattaag
     4261 gtatcctagc tccggaggac accccatgaa aatgacgatc ggaaagaagc tggcgttcgg
     4321 attcggggcg atcctcgccc tcgtcgtggc ctatggggtg acgatgatcg ttgagctgcg
     4381 gggcctggag cgcagctccc agaccatgac cacgcggagc gacaacgagc ttaagatcgc
     4441 cgtcgccgcc aggctgggcc ccctcatcta ccagaccatc gccgacgccg tgatcaaccg
     4501 cggcgacctc caggccatcg aaaagcgctg gaccgaagtg aaggcctccg ccatcgccga
     4561 gctcgagtcc atccgccccg ccctcgcgac cgagaccgag cgcgagcagc tcgccgaggc
     4621 cgagcggcgg ctcgccgagg tggccacgaa gttcgagggc ggcaccctgg tccagctcga
     4681 ccggggagcc cgggccgact tggagctcgt ctcgcgcatc gacggcgaca t
//
LOCUS       DASSQV010000005         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1742272, whole genome shotgun sequence.
ACCESSION   DASSQV010000005 DASSQV010000000
VERSION     DASSQV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742272"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1030)
                     /locus_tag="VFL04_00100"
     CDS             complement(77..1030)
                     /locus_tag="VFL04_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7838126.1"
                     /translation="MRNLRTSFAALALLALVLVVAGCAKPAPKETYIPLISKGFQHQF
                     WQAVKLGAEKAAADFKVTITFEGPETEAMVDKQMDMLSAALAKKPAAIGFAALDSKAA
                     IPLLQKAKDAKIPVIGFDSGVDSDIPVTTCATDNIAAAGYAAQMMAKLIGDSGEVAVI
                     VHDQTSRTGIDRRDGFVKEMEKHPNIKIVDIQYGGGDQLKSTDLAKSIIQAHPKLKGF
                     FGANEGSIIGVLNGVKEMKKVGKIVVIGYDAGKQQKDAIRSGEEAGAISQDPVGIGYK
                     TVEAAVKALKGEKLPKVIDTGFYWYDKTNIDDPTIQAVLYK"
     gene            complement(1098..2090)
                     /locus_tag="VFL04_00105"
     CDS             complement(1098..2090)
                     /locus_tag="VFL04_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7838127.1"
                     /translation="MSSTAAESGSAIKNALTANAMQKVLAFASLVVLMLIFSLASPNF
                     FQADNLIAIMLATAVNGVLALGVTFVIISAGIDLSVGTLMTFCAVMAGVFLTYWRLPI
                     WVGVIGAMASGMVVGSFSGFLIAKLKIPPFIATLGMLNVTKGLSLIISHLKPIYFNDA
                     PGFTNLALGTAVDIPGFAIPNAVIIFFALAILMSLVLNKTILGRYTYALGSNEEAVRL
                     SGVNVDRWKIVVYMLCGLICGIAGILIGARLNSAQPGLGQGYELDAIAAAVIGGTSLS
                     GGEGTILGTVIGAFIMSVLTNGLRILSVPQEWQTVVTGGIIIFAVYMDILRRRK"
     gene            complement(2100..>3240)
                     /locus_tag="VFL04_00110"
     CDS             complement(2100..>3240)
                     /locus_tag="VFL04_00110"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET7838128.1"
                     /translation="FESLNLRLDPRTKIMDMTVAKQQMVEIAKALSYEAKVLIMDEPT
                     AALNNAEIEDLFRIIRQLKAAGVGVAYISHRMDELKEISDRVTVMRDGAYIETLRTAE
                     TDIDHIIGLMVGRRLFETAKVDHGAHGNPVVLSVRDLRQGKTIKGVSFEVRAGEILGF
                     AGLMGAGRTEVARAIFGADPIDSGEIRVGGKPARIRSPSDAVRWGIGYLSEDRKRYGL
                     ATGMDLETNIVLATMRRFLGLFGILKESEMRATADRFVETLSIKTPGIRQKIKFLSGG
                     NQQKVVIAKWLVRDCQVLFFDEPTRGIDVGAKHEIYRLLNELAAGGKAIVMISSELPE
                     ILRMSHRIVVMCEGRITGELSAEEATQERIMQLATQRETMVAASS"
BASE COUNT          515 a         1015 c         1077 g          633 t
ORIGIN      
        1 gtccttccct ccgccggagc gagcggagct atcctgcgat agccccctcg ccccggcgcc
       61 cggcgtgcgg aacgccttat ttgtagagca cggcctggat ggtcgggtcg tcgatgttgg
      121 tcttgtcgta ccagtagaag cccgtgtcga tcaccttggg cagcttctcg cccttgagcg
      181 ccttgacggc cgcctcgacg gtcttgtagc cgatgccgac cgggtcctgg ctgatcgcgc
      241 cggcctcctc gcccgagcgg atcgcgtcct tctgctgctt gccggcgtcg tagccgatga
      301 cgacgatctt gccgaccttc ttcatctcct tgaccccgtt cagcacgccg atgatcgagc
      361 cctcgttcgc gccgaagaag cccttgagct tgggatgggc ctggatgatc gacttggcga
      421 ggtcggtgga cttgagctgg tccccgccgc cgtactggat gtcgacgatc ttgatgttcg
      481 ggtgcttctc catctccttc acgaagccgt cgcggcggtc gatgcccgtc ctgctcgtct
      541 ggtcatggac gatgacggcg acctcccccg agtccccgat gagcttggcc atcatctggg
      601 ccgcatagcc cgcggccgcg atgttgtcgg tggcgcaggt ggtcacaggg atgtcgctgt
      661 cgacgcccga gtcgaaaccg atgacgggga tcttcgcgtc cttggccttc tgcagcaggg
      721 gaatcgccgc cttgctgtcg agggcggcga agccgatggc cgccggcttc ttggcgaggg
      781 cggccgagag catgtccatc tgcttgtcga ccatggcctc ggtctcggga ccctcgaagg
      841 tgatggtcac cttgaagtcc gcggcggcct tctcggcccc gagcttgacc gcctgccaga
      901 actggtgctg gaagcccttg gagatgagcg ggatgtatgt ctccttgggg gcgggtttcg
      961 cgcaacccgc gaccacaagg accagggcga ggagcgccag cgccgcgaat gacgttctga
     1021 gatttctcat agcctctcct tcacgcaaag atggcctttc tcccgacggg agtttttcag
     1081 ggaccgtgga aactgcccta cttcctccgg cgcaggatgt ccatgtagac cgcgaagatg
     1141 atgatgcctc ccgtgacgac cgtctgccat tcctggggga ccgagaggat ccgcaggccg
     1201 ttcgtgagca cgctcatgat gaaggcgccg atcacggtgc caaggatcgt cccctcgcct
     1261 ccgctcaggg aagtgccgcc gatgacggcc gcggcgatgg cgtcgagctc gtagccctgg
     1321 cccaggcctg gctgggccga gttgaggcgg gctccgatca gtatgccggc gatgccgcag
     1381 atgaggccgc agagcatgta gacgacgatc ttccagcggt cgacgttgac gccggagagc
     1441 ctcaccgcct cctcgttgct gcccagggca taggtgtagc ggccgaggat cgtcttgttg
     1501 aggacgaggc tcatgaggat ggcgagggcg aagaagatga tgacggcgtt ggggatcgcg
     1561 aagccgggga tgtccaccgc cgtccccagg gcgaggtttg tgaagcccgg ggcgtcgttg
     1621 aagtagatcg gcttgaggtg ggagatgatg agggagaggc ccttggtgac gttgagcatg
     1681 cccagggtgg cgatgaaggg ggggatcttc agcttcgcga tcaggaaacc cgagaagctc
     1741 ccgacgacca tgccggaggc catggcgccg atcaccccga cccagatggg aaggcgccaa
     1801 taggtcagga agaccccggc catcacggcg cagaaggtca tgagggtgcc cacggagagg
     1861 tctatccccg cgctgatgat cacgaaggtg acaccgaggg cgaggacgcc gttgaccgcg
     1921 gtggcgagca tgatggcgat caggttgtcg gcctggaaga agttgggcga ggccagggag
     1981 aagatgagca tgaggaccac gaggctcgcg aaggccagga ccttctgcat cgcgttcgcc
     2041 gtgagcgcgt tcttgatcgc cgacccgctc tctgccgccg tactgctcat cggttcacct
     2101 caacttgatg ccgccaccat ggtctcgcgc tgggtggcga gctgcatgat cctctcctgg
     2161 gtcgcttcct cggccgagag ctcccccgtt atccgcccct cgcacatgac cacgatccgg
     2221 tggctcatgc ggaggatctc gggcagctcg gaggagatca tcacgatcgc cttcccgccg
     2281 gcggcgagct cgttaaggag tcggtagatc tcgtgtttgg ccccaacgtc gatgccgcgg
     2341 gtgggctcgt cgaagaagag gacctggcag tccctgacaa gccatttcgc gatgacgacc
     2401 ttctgctggt tgccgccgga aaggaatttt atcttctgcc tgatgcccgg cgtcttgatg
     2461 ctcagggtct ccacgaacct gtcggccgtc gcccgcatct cgctttcctt gaggatgccg
     2521 aacaggccca ggaagcgcct catggtggcc aggacgatgt tggtctcgag gtccatgccg
     2581 gtggccagac cgtagcgctt gcggtcctcg gagaggtagc cgatgcccca cctcaccgcg
     2641 tcgctcgggc tgcggatccg cgccggcttg ccgcccaccc tgatctcgcc cgaatcgatg
     2701 gggtcggccc cgaatatggc cctcgcgact tcggtccgtc cggcgcccat gaggccggcg
     2761 aagccgagga tctcccccgc cctcacctcg aacgacacgc ccttgatggt cttgccttgc
     2821 ctgagatcgc gcacgctgag gacgaccggg ttcccatggg cgccgtggtc caccttggcc
     2881 gtctcgaaga gcctgcgccc caccatgagg ccgatgatgt ggtcgatgtc ggtctcggcg
     2941 gtgcgcaggg tctcgatgta ggctccgtcg cgcatgaccg tgacccggtc ggagatctcc
     3001 ttgagctcgt ccatgcggtg cgagatgtag gccaccccta cgccggcggc cttgagctgg
     3061 cggatgatcc ggaaaaggtc ctcgatctcg gcgttgttga gggcggcggt gggctcgtcc
     3121 atgatcagca ccttcgcctc gtaggagagg gccttcgcga tctcgaccat ctgctgtttg
     3181 gccaccgtca tgtccatgat tttcgtccgg gggtcgagcc tgagattgag cgactcgaac
//
LOCUS       DASSQV010000006         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_251155, whole genome shotgun sequence.
ACCESSION   DASSQV010000006 DASSQV010000000
VERSION     DASSQV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251155"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1153
                     /locus_tag="VFL04_00115"
     CDS             260..1153
                     /locus_tag="VFL04_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET7838129.1"
                     /translation="MAAHASWKGFLKLSLVSVPVKAYTAAASGNEITLHQLHARTHAR
                     IQYKKFAPEVGEVSGDEIVKGYEYDKGQYVIIDDEELDKLRTESDKSIRIDGFIPPEA
                     LSPVYLGGKTYFLTPDGPVGQKPYRLLREGMEENKVCGIAEVVISQKEQVVLIQSIDG
                     LLAMTVLNRKDEVKSASAFKDELTDPEATEAERSLADTLIKASIIKDFDFSRYKDEYK
                     DKLTKLIQLKIEGKEVVQVHDPEEPKILNLMEALKRSVAEAQAAGGAKAGAKAEKAEL
                     KQAPSEKAAKPAAASKKKKVG"
     gene            1146..>2003
                     /locus_tag="VFL04_00120"
     CDS             1146..>2003
                     /locus_tag="VFL04_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838130.1"
                     /translation="MDRRLPEFVSPMLAVMGQPFDSPEFFFEFKWDGFRAAAYVEGGS
                     CRLVGRRGLDLTKGFLGLPRLASLGEGLVLDGELVAFVDGRPDFESVLGRGRPGKAAS
                     LSFIAFDLLYLGYESLMDRPFSERRARLEELMAPVRAQELLLSEGLGSQGKALYAKAC
                     ELGLEGVMAKRSSSAYAPGRRSPSWLKFKRRLEVQLAVIGYIDKGGRDFQCLLVAGSG
                     LPGEEEGSPLRYVGRVGGGFTERMRERLNGLLRERPRPRPLVACPEKGRWVEPGLYCR
                     VSYVELSSGG"
BASE COUNT          347 a          673 c          690 g          293 t
ORIGIN      
        1 gtgggtcggc gagggaggcg gggaggagcg ggcacaggct gtgccgctcc ttcctcgcgg
       61 ccgcgaggag ggcggcggcc tggccgtgga ggctcgggtc gtatttactg atgtcgagtc
      121 tgtccatgca tggcattgta cgacagccgg cctggcgttg actcccctcc ccccgcctcc
      181 gtactttcaa attacaggct ggccacagcc accgcgcatc gtccgatctt ccgcccgcca
      241 gcccccgaag gggaggacta tggcagccca cgcatcgtgg aagggtttcc tcaagctgag
      301 cctcgtgtcg gtgccggtga aggcctacac ggcggcggcg agcgggaacg agatcacgct
      361 ccaccagctc cacgcgagga cccacgcgcg catccagtac aagaagttcg cccccgaggt
      421 cggggaggtc tcgggcgacg agatcgtgaa gggctacgaa tacgacaagg gccagtacgt
      481 catcatcgac gacgaggagc tggacaagct gcgcaccgag agcgacaagt cgatccgcat
      541 cgacggcttc atcccgcccg aggccctgag cccggtctat ctcgggggca agacctactt
      601 cctcacgccc gacgggccgg tgggccagaa gccctaccgg ctcctccgcg agggcatgga
      661 ggagaacaag gtctgcggga tcgccgaggt ggtgatctcg cagaaggagc aggtggtcct
      721 catccagagc atcgacgggc ttctggccat gacggtgctc aaccgcaagg acgaggtgaa
      781 gagcgcctcg gccttcaagg acgagctcac ggaccccgag gccaccgagg ccgaacgcag
      841 cctcgccgac accctcatca aggcctccat catcaaggac ttcgacttct cgcgctacaa
      901 ggacgagtac aaggacaagc tcacgaagct catccagctc aagatcgagg gcaaggaggt
      961 ggtccaggtc cacgaccccg aggagcccaa gatcctcaac ctcatggagg ccctcaagcg
     1021 cagcgtggcc gaggcccagg ccgcgggcgg ggcgaaggct ggggccaagg cggagaaggc
     1081 cgagctcaag caggccccga gcgagaaggc cgcgaagccg gccgcggcgt ccaagaagaa
     1141 gaaagttgga tagacgcctc cccgagttcg tgtcgcccat gctggctgtc atgggacagc
     1201 ccttcgattc gcccgagttc ttcttcgagt tcaaatggga cggcttccgc gcagcggcct
     1261 acgtcgaggg cggctcctgc cgcctggtcg gccgcagggg cctcgacctc acgaagggct
     1321 tcctcggcct cccgcgcctc gcctccctgg gcgagggcct cgtcctcgac ggggagctcg
     1381 tggccttcgt cgacggccgg cccgacttcg agagcgtcct gggccgcggc aggccgggga
     1441 aggcggccag cctcagcttc atcgccttcg acctcctcta cctcggctac gagagcctca
     1501 tggaccggcc cttctccgag cggcgcgccc gcctcgagga gctcatggcg ccggtgcggg
     1561 ctcaggagct cctcctctcc gagggcctcg gctcgcaggg gaaggccctc tacgccaagg
     1621 cctgcgagct cggcctcgag ggggtgatgg ccaagcggag ctccagcgcc tacgccccgg
     1681 gccgtcgcag cccctcctgg ctcaagttca agcgcaggct cgaggtccag ctcgcggtga
     1741 tcggctacat cgacaagggg gggcgggact tccagtgcct cctcgtcgcg ggctcgggcc
     1801 ttcccggcga ggaggagggc tccccgctca ggtatgtcgg cagggtggga gggggcttca
     1861 ccgagcggat gcgcgaacgg ctcaacgggc tcctgcgcga gcggccccgg cctaggcccc
     1921 tcgtggcctg ccccgaaaag ggacgctggg tggaacccgg gctatactgc agggtgagct
     1981 atgtcgagct gagctcgggg ggc
//
LOCUS       DASSQV010000007         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_624644, whole genome shotgun sequence.
ACCESSION   DASSQV010000007 DASSQV010000000
VERSION     DASSQV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624644"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFL04_00125"
     CDS             <1..282
                     /locus_tag="VFL04_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HET7838131.1"
                     /translation="KHAFGEHSRKLKVSSTKSMTGHMLGAAGAAEAVISILALTRGIL
                     PPTINLENPDPECDLDYIPGAAREARVGAAISNSFGFGGHNCVLCFGRV"
     gene            complement(293..943)
                     /locus_tag="VFL04_00130"
     CDS             complement(293..943)
                     /locus_tag="VFL04_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET7838132.1"
                     /translation="MKDETEAILASFLRRRSELEVCASDLRAAFLALERCFRSGGKLL
                     VCGNGGSAADADHIVGELMKGFLLKRPLTQADREALLMSSAVHGPRLADSLQRALPAM
                     SLCGAGAFATAFANDVSGELAFAQAVRGWGRKGDALLALSSSGNSRNVVCAAVAAKAF
                     GLTTVALTGRGGGELVAICDVTIRVPAHETFLVQELHLPVYHALCAMLEEEFFGGD"
     gene            complement(940..1929)
                     /locus_tag="VFL04_00135"
     CDS             complement(940..1929)
                     /locus_tag="VFL04_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7838133.1"
                     /translation="MDDMYGIGFDIGGTKCAVSLGLIDGGSLKILSRSEYPTAGGPGP
                     CLARATSLVEAALASAGIGRGQLGAIGISCGGPLDLERGLVLSPPNLGGWDGVEVVEH
                     FRGLCDRVVLQNDANASALAEWRYGAGRGLDSLVFLTFGTGLGAGIILGGRLWSGASG
                     MAGELGHIRLADFGPVGYGKTGSFEGFCSGGGIAQLARIKVAERLQRGETVSLCAEPG
                     GLDALDAKTVAEAARRGDSLAIEILELSGTYLGRGLSILVDLLNPEAIILGGIFMRCE
                     DFLRPAMEGVLARESLGRSLEACRIMPAGLGERVGDYAALAVAFHGRDGGRAS"
     gene            complement(2027..>2207)
                     /locus_tag="VFL04_00140"
     CDS             complement(2027..>2207)
                     /locus_tag="VFL04_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838134.1"
                     /translation="SSVASGSGAIGPAIGATLATAAGYPSVFLATGAILAASGFGIAM
                     FVRRPKMGPSSSSSA"
BASE COUNT          347 a          803 c          775 g          282 t
ORIGIN      
        1 aagcacgcct tcggcgagca ctcgaggaag ctcaaggtct cctcgaccaa gagcatgacc
       61 ggccacatgc tgggcgcggc cggcgccgcc gaggcggtga tcagcatcct cgcgctcacc
      121 cgcgggatcc tgccgcccac catcaacctc gagaaccccg accccgaatg cgacctcgac
      181 tacatccccg gcgccgccag ggaggcgagg gtcggcgcgg ccatatccaa ctccttcggc
      241 ttcggcggac acaactgcgt gctctgcttc gggagggtct aggacgggcg cttcagtccc
      301 ccccgaagaa ctcctcctcg agcatggcgc agagggcgtg atagaccggc aggtggagct
      361 cctggaccag aaaggtctcg tgcgcgggga cgcggatcgt gacgtcgcag atcgcgacga
      421 gctccccccc gccccgaccc gtgagggcta ccgtcgtcag gccgaaggcc ttcgccgcca
      481 cggcggcgca cacgacgttg cgggagttgc ccgagctgct cagggcgaga agggcgtccc
      541 ccttcctccc ccagccccgc acggcctggg cgaaggcgag ctcccccgag acgtcgttgg
      601 cgaaggcggt ggcgaaggcc cccgccccgc agagcgacat cgccggcagg gcccgctgga
      661 gcgagtcggc gagccggggc ccgtggacgg ccgagctcat gaggagggcc tcgcggtcgg
      721 cctgggtcag gggacgcttg aggaggaaac ccttcatgag ctcgccgacg atgtggtcgg
      781 cgtcggcggc gctccccccg ttcccgcaga ccaggagctt cccgcccgag cggaagcatc
      841 gctcgagggc gaggaaggcg gcgcgcagat ccgaggcgca gacctcgagc tcgctccgac
      901 gccgcaggaa actggcaagg atcgcctcag tctcgtcctt catgaagccc tccctccatc
      961 ccggccgtgg aaggccacgg cgagggccgc gtagtcgccc accctctcgc cgagccctgc
     1021 cggcatgatc cggcaggcct caaggctccg gccaaggctt tccctcgcga ggaccccctc
     1081 catcgccggc ctgaggaagt cctcgcagcg catgaatatg ccgccaagga tgattgcctc
     1141 gggattgagc aggtcgacca ggatcgagag ccccctgccg aggtaggtcc cgctgagctc
     1201 gaggatctcg atggcgaggc tgtcgccgcg tctggccgcc tcggccaccg tcttcgcgtc
     1261 cagggcatcg aggccgcctg gctcggcgca gagcgacacg gtctcgcccc tctggagccg
     1321 ctcggcgacc ttgatcctcg ccagctgggc gatgccgccc ccggagcaga agccctcgaa
     1381 ggagcccgtc ttgccatagc ccaccgggcc gaaatcggcg aggcggatgt ggccgagctc
     1441 gcccgccatg ccgctcgcgc ccgaccagag ccgtccgccc aggatgatgc ccgccccgag
     1501 ccccgtgccg aaggtgagga agacgaggct gtcgaggccc ctgccagccc cgtagcgcca
     1561 ctcggcgagg gcggaggcgt tggcgtcgtt ctgaaggacc accctgtcgc agagtccgcg
     1621 gaaatgctcg accacctcga ccccgtccca gccgcccaga ttggggggcg agaggacgag
     1681 gccccgctcg aggtcgaggg ggccgccgca gctgatcccg atggcgccga gctggccgcg
     1741 gccgatgccg gccgaggcca gggccgcttc cacgaggctg gtggcccgcg ccaggcaggg
     1801 gccgggtcca ccggctgtcg ggtactccga cctcgagagg atcttcagag accctccgtc
     1861 gatcagcccg aggctgacgg cgcacttggt cccgccgatg tcgaagccga tcccgtacat
     1921 gtcgtccatg ataccagagc ggggcccgtg gcgccagaat cgccgccgcc ccgagctccc
     1981 gcgcgtgggg cggcgctcgg atcggccggc ggctcagggg ccgcggctag gccgaggagc
     2041 tggaggaggg gcccatcttg ggcctgcgca cgaacatcgc gatgccgaag cccgaggccg
     2101 cgaggatggc ccccgtggcc aggaagaccg agggatagcc cgcggcagtc gcgagggtcg
     2161 cgccgatggc ggggccgatg gccccgctcc ctgaggcgac cgagctg
//
LOCUS       DASSQV010000008         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1494433, whole genome shotgun sequence.
ACCESSION   DASSQV010000008 DASSQV010000000
VERSION     DASSQV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494433"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFL04_00145"
     CDS             <1..865
                     /locus_tag="VFL04_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7838135.1"
                     /translation="YLPMVTSYTEKDFGGEFATEADYRDPVVQADIKAKGWMLWPPLP
                     FSYSTINYDLPSPAPSPPTRVNPIGTDEKGHDVLAILIYGFRLSVIFGLTLSILSSMI
                     GVAAGAVQGYYGGRIDILGQRFIEIWSGMPTLFLLIILSSVVQPNFWWLLLITLLFGW
                     MGLVGVVRAEFLRARNFEYVQAARALGVSNARIIFRHILPNAMIATLTFMPFILGGSI
                     TTLTSLDFLGFGMPVGSPSLGDLLAQGKDNLQAPWLGISTFVVLAGTLSLLVFVGEAV
                     RDAFDPRRAYK"
     gene            862..>2459
                     /locus_tag="VFL04_00150"
     CDS             862..>2459
                     /locus_tag="VFL04_00150"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7838136.1"
                     /translation="MNAEPQGIQGRLGGDPGRLGGGQPGEAAGAAAPKLVEIRNLRIA
                     FRKGASLTEVVHGASLSISRKETLALVGESGSGKTVTAQAILRLLPESLIAYPEGEVL
                     FEGTDVLRMSNQGLLGIRGDRVGMIFQEPMTSLNPLHTVERQLNEALLLHRGLPPSKA
                     SPLSLEWLSRVGLEAPEKKLKSYPHQLSGGERQRVMIAMALVNEPDLLIADEPTTALD
                     VTIQAQILRLIKQLQAELGMAVLFITHDLGIVRRVADRVAVMHDGHIVETGETRAIFA
                     HPQHEYTRKLLAAEPRPEAQVGEPQRPVVLRARGLKVWFPIQTGLLRRTTDYVKAVDG
                     IGLELRAGQTLGVAGESGSGKTTLGRAILRLVKSEGEIEFEGMSVHQMGSEELRALRR
                     RMQIIFQDPFGSLNPRMSVEQIVGEGLEVNNMGSRDEREAAIVDTMREVGLDPEHRHR
                     YPHEFSGGQRQRIALARALVLKPELLILDEPTSSLDRTIQFQVTELLRELQAAHSLSY
                     IFISHDLKVLKSLCHDLIVMRRGRV"
BASE COUNT          414 a          889 c          774 g          382 t
ORIGIN      
        1 ctacctgccg atggtgacga gctacaccga gaaggacttc gggggggagt tcgccaccga
       61 ggccgactac cgcgaccccg tggtccaggc cgacatcaag gccaagggct ggatgctctg
      121 gcccccgctc cccttcagct acagcaccat caactacgac ctgccgagcc ccgccccctc
      181 cccgccgacg agggtgaacc ccatcggcac cgacgagaag ggccacgacg tcctcgccat
      241 cctcatctac ggcttcaggc tctcggtgat cttcgggctg accctgagca tcctctcgtc
      301 catgatcggg gtggccgcgg gggcggtcca gggctactac ggggggcgca tcgacatcct
      361 gggccagagg ttcatcgaga tctggtcggg catgccgacc ctcttcctcc tcatcatcct
      421 ctcgagcgtc gtgcagccca acttctggtg gctcctcctc atcaccctcc tcttcggctg
      481 gatgggtctc gtgggcgtcg tgagggccga gttcctccgg gcccgcaact tcgagtacgt
      541 ccaggccgcg cgggccctgg gggtgagcaa cgcgaggatc atcttcaggc acatcctgcc
      601 caacgcgatg atcgccaccc tgaccttcat gcccttcatc ctgggcggct cgatcacgac
      661 cctgacctcg ctcgacttcc tgggcttcgg catgcccgtg ggctcgccct cccttggcga
      721 cctcctggcc cagggcaagg ataacctcca ggccccctgg ctcgggatca gcaccttcgt
      781 tgtcctcgcc ggcaccctga gcctcctggt cttcgtgggc gaggccgtcc gcgacgcctt
      841 cgatccgagg agggcctaca aatgaatgcc gagccacaag ggattcaggg tcggctcggc
      901 ggggatccgg gtcggctcgg cgggggccag cccggcgagg ccgccggcgc cgccgcgccg
      961 aagctcgtcg agatcaggaa cctgcgcatc gccttccgca agggcgcttc cctgaccgag
     1021 gtggtccacg gcgccagcct gagcatcagc cgcaaggaga ccctcgccct cgtgggggag
     1081 agcggctcgg gcaagaccgt gaccgcccag gccatcctca ggctccttcc ggaatcgctc
     1141 atcgcgtacc ctgagggcga ggtcctcttc gaggggaccg acgtgctcag gatgtcgaac
     1201 cagggcctgc tcggcatacg gggcgaccgg gttggcatga tcttccagga gccgatgacc
     1261 tcgctcaacc ccctgcacac ggtcgagcgc cagctgaacg aggccctcct cctccaccgc
     1321 ggcctgccgc catccaaggc cagccccctg agccttgagt ggctctcccg ggtgggcctc
     1381 gaggcgcctg agaagaagct gaagtcctac ccccaccagc tctcgggcgg ggagaggcag
     1441 agggtcatga tagcgatggc cctcgtcaac gagcccgacc tcctgatcgc agacgagccg
     1501 accacggccc tcgacgtcac gatccaggcc cagatcctca ggctcatcaa gcagctccag
     1561 gccgagctcg ggatggccgt cctcttcatc acccacgacc tcggcatcgt aaggcgggtg
     1621 gccgaccgcg tagccgtcat gcacgatggc cacatcgtcg agacgggcga gaccagggcc
     1681 atcttcgccc atccccagca cgagtacacg aggaagctcc tcgccgccga gccgcggccc
     1741 gaggcccagg tgggcgagcc gcagcggcct gtggtcctca gggccagggg cctgaaggtc
     1801 tggttcccca tccagacggg gctgctcagg aggacaaccg actacgtgaa ggccgtggac
     1861 ggcatcggcc tcgagctccg cgcaggccag accctggggg tggccggcga gagcggctcg
     1921 ggcaagacca ccctcggccg cgccatactg cgcctggtaa agagcgaggg cgagatcgag
     1981 ttcgagggca tgagcgtgca ccagatgggg agcgaggagc tgcgcgccct caggcgccgc
     2041 atgcagatca tcttccagga ccccttcggc tcgctgaacc cgcggatgtc ggtcgagcag
     2101 atcgtgggcg agggcctcga ggtgaacaac atgggaagcc gcgatgagcg cgaggcggct
     2161 atcgtcgaca cgatgcgcga agtcggcctc gatcccgagc accgccaccg ctacccccac
     2221 gagttctcgg gcgggcagag gcagcgcatc gccctcgcga gggcactggt cctcaagccc
     2281 gagctcctca tcctcgacga gcccacctcc tccctcgaca ggacgatcca gttccaggtg
     2341 acagagctcc tgcgcgagct ccaggccgcc cattccctga gctacatctt cataagccac
     2401 gacctcaagg tgctcaagtc cctctgccac gacctcatcg tcatgcgccg cggcagggt
//
LOCUS       DASSQV010000009         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_624826, whole genome shotgun sequence.
ACCESSION   DASSQV010000009 DASSQV010000000
VERSION     DASSQV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624826"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFL04_00155"
     CDS             complement(<1..208)
                     /locus_tag="VFL04_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838137.1"
                     /translation="MKRKALLASAILAIAAVAAPLFAAPVVSEKKDVAIFALGYYGFA
                     IPMEGLGNIDSEIQKVFVDLGRFNI"
     gene            376..1758
                     /gene="mnmE"
                     /locus_tag="VFL04_00160"
     CDS             376..1758
                     /gene="mnmE"
                     /locus_tag="VFL04_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET7838138.1"
                     /translation="MQRSYLDEPVPIVAAATPPGESALAVIRAAGPGCLALAARCFSR
                     PRALLEAPGHSSVYGRLVEPSSGAIVDEVLVSVFRAPRSPTGEDGVEFSCHGSPAVVR
                     KALGLLEAAGFAPALPGEFSFRAFLHGKTDLVRAEAVAELIRSAADAARAEAIRRLEG
                     GLSARIGRARAELLAVLAEVQVRLDFDEDQGAPEAELDESALARVAESLAALSDSYAF
                     GRLYAEGARVVVAGRPNAGKSSLFNLMLREERSIVSHEPGTTRDWIEAGLEVEGLPVR
                     LIDTAGLREAGAAAEAEGVSRSRRLLAQADAVIYLVDGVTGLAPEDEAFLSSYAGKGL
                     VPAWNKVDHPSCRAAPEGFVALSARSGAGLQALASALARAILSAAGAADAGLREDEVR
                     VASLRQKGLLDRALQSIREAQAGLSAGAPLDALSLDIGAAADALGEITGEISSAEILE
                     AIFSGFCLGK"
     gene            1767..>2755
                     /locus_tag="VFL04_00165"
     CDS             1767..>2755
                     /locus_tag="VFL04_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7838139.1"
                     /translation="MDYEAIVIGGGHAGIEAALALARLGHRTLMITQDLEAIGRLSCN
                     PAVGGLSKGNLVREIDALGGQMGLLIDASMIQYRMLGRSRGPAVQSPRAQADKALYAS
                     LARRALEAQRGLSLFMDTVVDLLTDGSGGKVEGVLTARGNRIGASCVVLTTGTFMEGR
                     LFIGDWRASGGRLGESAALGLGTALRGRGFELGRMKTGTPARIRAGSIDTGEMARQEG
                     EAARPFSFLNDSIERPEASCWVTYTNEETHRVIRGGMDRSPLFSGAIVGMGPRYCPSI
                     EDKVRRFPERERHQVFVEPEGLSTDEMYLNGLSTSLPEEVQRDFIRTIPGLRRA"
BASE COUNT          396 a          994 c          946 g          419 t
ORIGIN      
        1 tgatgttgaa tcggcccaga tcgacgaaga ccttctggat ctcgctatcg atgttgccca
       61 gcccctccat ggggatcgcg aagccgtagt agccgagggc gaagatcgcg acgtccttct
      121 tctcgctgac cacgggggcc gcgaagagcg gggccgccac ggcggcgatg gccaaaatcg
      181 ctgacgcgag caatgccttg cgtttcattt ttcctcctag cggggatcgg gggccggagc
      241 tcggacttgc ggattacccg gcccctccga taatccattc ttagcaccag cccccctcct
      301 gtcaatagaa ggcggccccg gccagcagcg ggccttgcgc gcggggctcg atacggacta
      361 tcctgcggtg atcccatgca gcgctcctac ctcgacgaac ccgtccccat agtcgccgcg
      421 gcgacaccgc ccggggaatc ggccttggcc gtcatccgcg ccgccggccc cggctgcctc
      481 gccctggctg cgcgttgctt ctcccggcct cgggccctcc tcgaggctcc agggcacagc
      541 tcggtctacg ggaggctggt cgagccttcc tcgggcgcca tcgtggacga ggtcctcgtc
      601 tcggtgttca gggccccgcg gagtcccacg ggcgaggacg gtgtggagtt ctcctgccac
      661 ggctcccccg ccgtcgtgag gaaggccctg ggcctcctcg aggccgccgg tttcgccccc
      721 gccctgccgg gggagttctc cttccgcgcc ttcctccacg gcaagaccga cctcgtgcgc
      781 gccgaggccg tcgccgagct catccgctcc gcggccgacg ccgcgcgcgc cgaggcgatc
      841 cgaaggctcg agggcgggct ctcggcgagg atcggaaggg cgagggccga gcttttggcc
      901 gtcctggccg aggtccaggt gaggctggac ttcgacgagg accagggggc gcccgaggcc
      961 gagcttgacg agtccgccct ggcccgcgtg gccgagtccc tcgctgccct ctccgacagt
     1021 tatgccttcg ggaggctcta cgccgaggga gcccgggtgg tcgtggcggg ccggcccaac
     1081 gccggcaagt cgagcctctt caacctcatg ctccgcgagg agaggtcgat cgtgagccat
     1141 gagcccggga ccacccgcga ctggatcgag gccgggctcg aagtcgaggg ccttccggtc
     1201 aggctcatcg acacggccgg gcttcgcgag gcgggggcgg ccgccgaggc cgagggggtc
     1261 tcgaggagcc gccgcctcct cgcccaggcc gacgccgtca tctatcttgt cgacggcgtc
     1321 acgggcctcg cacccgagga cgaggccttc ctttcctcgt atgccgggaa gggcctcgtg
     1381 ccagcatgga acaaggtcga ccatccctcc tgccgcgcgg cgcccgaggg cttcgtggcc
     1441 ctgagcgcga ggagcggagc tggccttcag gccctcgcct cggccctggc aagggcgatc
     1501 ctctcggcgg ccggcgccgc cgacgccggg ctgagggagg acgaggtcag ggtcgcgagc
     1561 ctgcgccaga aaggcctcct tgaccgcgcc ctccaatcaa tcagggaggc ccaggccggc
     1621 ctgtcagcgg gcgcccccct cgacgccctc tccctcgaca tcggcgcggc cgccgacgcc
     1681 cttggcgaga tcaccggcga gataagctcg gccgagatcc tcgaggcgat cttctccggc
     1741 ttctgcctcg ggaagtaggg accagagtgg actacgaggc catcgtcata ggcggagggc
     1801 acgcggggat cgaggcggcc ctggccctcg cgcgcttggg ccacaggacc ctcatgatca
     1861 cccaggacct cgaggccatc ggacgcctct cctgcaaccc cgccgtgggc ggtctgtcca
     1921 agggcaacct cgtccgcgag atcgacgccc tcggcgggca gatgggcctc ctcatcgacg
     1981 cctccatgat ccagtaccgc atgctcgggc gctcccgcgg gccggccgtc cagtcgcccc
     2041 gggcccaggc cgacaaggcc ctctacgcga gcctggcgcg ccgggccctc gaggcgcaga
     2101 ggggcctctc cctcttcatg gacaccgtcg tcgacctcct caccgacggc tcgggcggga
     2161 aggtcgaggg ggtcctcacc gcccgcggca acaggatcgg cgcctcctgc gtggtcctca
     2221 ccaccgggac cttcatggag ggccggctgt tcatcggcga ctggagggcc tcgggaggac
     2281 ggctcggcga gagcgccgcc ctggggctcg gcaccgccct gcgaggtcgg ggcttcgagc
     2341 ttggccgcat gaagacgggg actccggcga ggatcagggc aggctcgatc gacaccggtg
     2401 agatggcgag gcaggagggc gaggcggcga ggcctttctc cttcctgaac gactccatcg
     2461 agaggcccga ggcctcctgc tgggtcacct acaccaacga ggagacccac cgcgtgatcc
     2521 ggggcgggat ggacaggtct ccccttttca gcggggccat cgtcggcatg ggaccgaggt
     2581 actgcccctc gatagaggac aaggtgaggc gcttccccga gcgcgagcgc caccaggtct
     2641 tcgtcgagcc cgaagggctc tcgaccgacg agatgtacct caacgggctc tccacctccc
     2701 tgcccgagga ggtccagcgc gatttcatca ggaccatccc cgggcttcgg cgtgc
//
LOCUS       DASSQV010000010         6937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_997711, whole genome shotgun sequence.
ACCESSION   DASSQV010000010 DASSQV010000000
VERSION     DASSQV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997711"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1035
                     /gene="ispH"
                     /locus_tag="VFL04_00170"
     CDS             178..1035
                     /gene="ispH"
                     /locus_tag="VFL04_00170"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET7838140.1"
                     /translation="MIVKRAEVLGRCMGVRRAVDLAMATADSDPSRPVYTLGPLIHNP
                     QAVAELEARGVIALASSDIDERVRGKSVVIRAHGVPPLLRSRLEELGARVVDATCPRV
                     IASQRKARDYAAKGWKVIIAGDRDHGEVAGIAGYASAGWAVVGSSAEAEALELEGKAV
                     VIAQTTIKTEEYKAICEVIARRFQAVELVDSICPATEDRQRALGRLAAETDALVVVGG
                     RNSANTSRLFTTALALGKPAWHVETAAELPEEVFAYTRVGVTAGASTPESLVAEVEAA
                     LVSRGAAAT"
     gene            1056..1628
                     /locus_tag="VFL04_00175"
     CDS             1056..1628
                     /locus_tag="VFL04_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7838141.1"
                     /translation="MDIISEIEKRRAFRSMSGEALDEEVVERMLRAATLAPSCFNSQP
                     WRIFAVMGERLPVLREALSANNKWATAAPLIVVMATKPSLDCRLDGGRDYAFCGLGLA
                     AMNLMLQATREGLYAHPIAGFSPAKAAKAIDLPEDFVPLTLIIVGRPGSSELLSESQL
                     EREHSGRERKALGEVAFRDSWGSPWGAQGG"
     gene            1680..2297
                     /gene="udk"
                     /locus_tag="VFL04_00180"
     CDS             1680..2297
                     /gene="udk"
                     /locus_tag="VFL04_00180"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET7838142.1"
                     /translation="MANARIIGICGGSGSGKTTIVRKISEVVSDFVFLPQDNYYKSAE
                     YISNSNITAFNFDHPDAFDNALLVEHLDALKRGEGIEMPTYDFVHHRRTDETIKIEAR
                     KLVIFEGIMIFTNREVRDRIDLKVFVDTPDDIRFIRRLTRDIKERGRTLDSVIEQYLT
                     VVRPGHYEFIEPTKQFADIIIPEGGANERALDVLVTFINSVVRSD"
     gene            2476..3867
                     /locus_tag="VFL04_00185"
     CDS             2476..3867
                     /locus_tag="VFL04_00185"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7838143.1"
                     /translation="MTYSNTRRLVEGTATLAAAAAFIIVPIVASAELRPATVVVFGAV
                     AFTVLFALRNSGATTADRALYRSRFRQPQTRLILDFADRIGTPFTIPDLVEAIREKLE
                     RQGDMGVILVRSNTWEIMYQSPSAAASDQKTLETLQRNFREWSEGFCFINDELSLTSS
                     NSDARGFFLNVKGFGFFVLTRLCPLIELEAFRALHGELRIYFDRVLTISDLFEVASLS
                     KEWELIAETQRSFLPKSLPAIKKLDLAVNYRPLVNVSGDYYDVIQVDEDRTLMVVGDV
                     SGKGLAAALIMGIIVNTIRVTRDKADLANLIRSVDSAVRDMGFDDKYTVIFLGIVDTK
                     NRTLRYVNAAMADPIIISQTALGPRVRRLEPTMGLVGLIPLERIDVEELPLRTDEIIL
                     LASDGVTEVEGEGGERLGETELFEKTIAQASKLGATDFVASTSGLLYSFVGDKPLKDD
                     VTILAAKVGRLWD"
     gene            3858..6011
                     /locus_tag="VFL04_00190"
     CDS             3858..6011
                     /locus_tag="VFL04_00190"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET7838144.1"
                     /translation="MGLTLLSFLVFPAFLANILIVRRRFRGAEGLRITEGVSLSFAIY
                     ALLQSLSLAFISAAAMPHLSHNLIKTSAFFFGLSYILIFVFVLDFPVPLPRAVRIVGG
                     ILATAAAALALWRIVFTPDYILATVRAGSDIVRVDGPQYGLINNLETGLAALSAAILL
                     ARSLGIKSRIQRQRGLVAFAGVVVGAGFIWLLPQLVVSRSVVRSVFALTPVAALVLAS
                     VVTYAFYLSRIFDWRSIGRQLAYYALLTLVVGLPTGFVVYVLTLLRIVSETVPLVGTF
                     LVFFVSLYLARGFSARFLARSGAPGEYREELESGLAHIDLAEGRDLVLGKLYKLLSAA
                     LDFSDFTVLIEDDQGSLKSVFSPQGSKALIEKGSDLAVALDRSGATVLLKSEALAHPA
                     YEDVRAEFMSLFESLNAEAFILAREGRHIIGAFILGARRTGADFTDYDYDTFKSIYGK
                     LFVFAYYLKNVARESLLYTVDREIALSDQIIRFALEKVDTFRHPKVDAAWALRYMRTL
                     GGDFIDFVRLSPDRWFFVMGDVSGKGLSASMNMLILKSMIRTFLRVEKDFVSLVSRIN
                     SFIKENLPRGNFFAGIFGYFDLAKDAFYYINCGIPALLLYSPSFDAFIEVQGEGKILG
                     FVRDIKPYLKPRKLVLPPGCVLVAATDGITDSENLRGERFGKERLRRSIQDRLGQASN
                     EIVGGVLDDLLSFSDRKQDDDLTLFVIKFPPRSAE"
     gene            6008..6331
                     /locus_tag="VFL04_00195"
     CDS             6008..6331
                     /locus_tag="VFL04_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7838145.1"
                     /translation="MTQLDITTRNEEGHEILVVSGPINSYTFTEFQEKVYKAIRAGDT
                     IIDMEKVTTLSSAGIGVLMAALDDGEAEGHRLVVLKPSEIVRLAMDSTGFADRFPVIQ
                     TLREF"
     gene            6430..6888
                     /locus_tag="VFL04_00200"
     CDS             6430..6888
                     /locus_tag="VFL04_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838146.1"
                     /translation="MVLEGFVDGLDFLARRERHRIRLAGGEILDNLVRHSAPLAGGSL
                     SVRVSRRRTGIVMAFFFRSPKFAAFAARCSDPEPLFDPAHRRWRGIGMVMCRNLSSSM
                     RLRPGRFIDRIFLSFRLEPDAHTDPAGRPGMELGPEGPGQSTGRARLAEK"
BASE COUNT         1105 a         2599 c         2092 g         1141 t
ORIGIN      
        1 agcgccctct tccgcaagcc aaagaggcgg ccccgagggg ccctggcggg ttccgggggg
       61 cccgggcggg ttccgaggag cccgggcggg tttcgggggg cccgggcgga acccgaaggg
      121 gctggcgccc cgcccggcgg gcttctatca tccccaagcc ttcaatatac tcgtcccatg
      181 atagtcaagc gcgccgaggt cctcggcagg tgcatgggcg tcaggagggc cgtcgacctc
      241 gccatggcca cggccgactc cgacccctcc cgccccgtct acaccctcgg ccccctcatc
      301 cacaaccccc aggccgtcgc cgagctcgag gcccgcgggg tcatcgccct cgcctcctcg
      361 gacatcgacg agagggtgcg gggcaagtcg gtcgtgatca gggcccacgg ggtccccccc
      421 ctcctgcgct cccggctcga ggagctcggc gccagggttg tggacgccac ctgcccccgg
      481 gtcatcgcca gccagaggaa ggcccgcgac tacgccgcca agggctggaa ggtgatcatc
      541 gccggggacc gcgaccacgg cgaggtggcg ggcatcgcgg gctacgcgag cgccggctgg
      601 gccgtcgtgg gctcgagcgc cgaggccgag gccctcgagc tcgagggcaa ggccgtggtc
      661 atcgcgcaga ccacgatcaa gaccgaggag tacaaggcca tctgcgaggt catcgcgagg
      721 cgcttccagg ccgtcgagct cgtcgactcg atctgcccgg ccaccgagga ccgccagagg
      781 gccctcggcc gcctggccgc cgagaccgac gccctcgtgg tcgtgggggg ccgcaactcg
      841 gcgaacacga gccggctctt cacgacggcc ctggccctcg gcaagcccgc ctggcacgtc
      901 gagaccgcgg ccgagctgcc cgaggaggtc ttcgcctaca cccgggtggg ggtgaccgcg
      961 ggggcgagca cccctgagag cctggtggcc gaggtcgagg ccgccctcgt gtcccggggc
     1021 gcggccgcca cctagggggg ccaaggaggt tttccgtgga catcataagc gagatcgaga
     1081 agcgccgcgc attcaggtcc atgtcgggcg aggccctgga cgaggaggtc gtggagcgca
     1141 tgctccgcgc cgccaccctc gccccctcct gcttcaacag ccagccctgg cggatcttcg
     1201 ccgtcatggg ggagcgcctc cccgtgctga gggaggccct gagcgccaac aacaagtggg
     1261 ccacggcggc ccccctcatc gtcgtgatgg ccaccaagcc atccctggac tgccggctcg
     1321 acggcggccg cgactacgcc ttctgcggcc tcggcctggc cgccatgaac ctcatgctcc
     1381 aggcgacccg ggaggggctc tacgcccacc cgatcgccgg cttctcgccc gccaaggcgg
     1441 ccaaggccat cgacctgccc gaggacttcg tccccctcac cctcatcatc gtgggcaggc
     1501 ccggctcgag cgagctcctc tcggagtccc agctcgagcg ggagcacagc gggcgcgagc
     1561 gcaaggccct cggcgaggtc gccttccgcg actcctgggg ctcgccctgg ggggcccagg
     1621 gcggctgagg cgcgaaaaaa ggctgtacaa ggggagccca tccggttact ctgtcgccca
     1681 tggccaatgc gcgtatcata ggcatctgcg gcggctcggg ctcgggcaag accacgatcg
     1741 tcaggaagat atccgaggtg gtctcggact tcgtcttcct cccccaggac aactactaca
     1801 agtccgccga gtacatctcc aactccaaca tcacggcctt caacttcgac caccccgacg
     1861 ccttcgacaa cgccctcctc gtcgagcacc tcgacgccct gaagcgcggc gaggggatcg
     1921 agatgcccac ctacgacttc gtgcaccaca ggcggaccga cgagacgatc aagatcgagg
     1981 cccgcaagct cgtcatcttc gaggggatca tgatcttcac gaaccgcgag gtcagggacc
     2041 gcatcgacct caaggtcttc gtcgacaccc ccgacgacat ccgcttcatc cgccgcctga
     2101 cccgcgacat caaggagagg ggccgcaccc tcgactcggt gatcgagcag tacctcaccg
     2161 tggtcaggcc cgggcactac gagttcatcg agcccaccaa gcagttcgcc gacatcatca
     2221 tccccgaggg cggggccaac gagcgcgccc tcgacgtcct ggtcaccttc ataaactcgg
     2281 tcgtcaggtc ggactagggg cgcctggccc ggttcccgct ctgcgattcc tgttgactgg
     2341 ccgatatttg gcatctaaaa atgatcagtt cgtggcgggc cggccctgga gccggcccaa
     2401 ggcactgatt tcggacccag acgggtttgg aaaggcccga cggatgccga taccgtatct
     2461 ggagtacctc aagagatgac ctacagcaac acccggcggc tcgtcgaggg gaccgcgacc
     2521 ctggccgcgg ccgcggcctt catcatcgtg ccgatcgtcg cctcggccga gctcaggccc
     2581 gcgacggtcg tcgtcttcgg ggcggtggcc ttcaccgtgc tcttcgccct caggaattcc
     2641 ggcgccacga cggccgaccg ggccctctac cggagccggt tccgccagcc ccagacccgg
     2701 ctcatcctgg acttcgccga ccggatcggc acccccttca ccatccccga cctcgtcgag
     2761 gccatcaggg agaagctgga gcgccagggc gacatggggg tcatcctggt gaggtccaac
     2821 acctgggaga tcatgtacca gagcccctcg gcggcggcca gcgaccagaa gaccctcgag
     2881 accctccagc gcaacttccg cgagtggtcg gagggcttct gcttcatcaa cgacgagctc
     2941 tccctcacct cctccaacag cgacgcgcgc ggcttcttcc tcaacgtcaa gggcttcggc
     3001 ttcttcgtcc tcacccggct ctgccccctc atcgagctcg aggccttccg cgccctccac
     3061 ggcgagctgc gcatctattt cgaccgcgtc ctcaccatct ccgacctctt cgaggtggcc
     3121 tcgctctcca aggagtggga gctcatcgcc gagacccagc gctccttcct gcccaagagc
     3181 ctgccggcga tcaagaagct cgacctggcc gtgaactacc gccccctcgt caacgtctcg
     3241 ggcgactact acgacgtgat ccaggtcgac gaggaccgca ccctcatggt ggtcggcgac
     3301 gtctcgggca agggcctcgc ggccgccctg atcatgggca tcatcgtcaa caccatccgc
     3361 gtcacccgcg acaaggccga cctcgccaac ctcatccgga gcgtggacag cgccgtcagg
     3421 gacatgggct tcgacgacaa gtacacggtc atcttcctcg gcatcgtgga caccaagaac
     3481 cggaccctgc gctacgtcaa cgccgccatg gccgacccga tcatcatctc ccagacggcc
     3541 ctggggcccc gggtccgcag gctcgagccc acgatgggcc tggtcggcct catccccctc
     3601 gagaggatcg acgtcgagga gctgcccctg cgcaccgacg agatcatcct cctcgcctcg
     3661 gacggggtca ccgaggtcga gggcgagggc ggggagcggc tcggcgagac cgagctcttc
     3721 gagaagacca tcgcccaggc ctcgaagctc ggcgccaccg atttcgtcgc gagcacctcg
     3781 ggcctcctgt actccttcgt gggcgacaag cccctcaagg acgacgtcac catcctcgct
     3841 gccaaggtag gaagactatg ggactgacgc ttctatcctt cctcgtcttc ccggccttcc
     3901 tcgcgaacat cctcatcgtc cgccggcgct tccgcggggc cgagggcctc aggatcaccg
     3961 agggcgtctc cttgagcttc gccatctacg ccctcctcca gagcctctcg ctggccttca
     4021 tctccgccgc ggccatgccc catctctcgc acaacctcat caagacctcg gccttcttct
     4081 tcggcctcag ctacatcctc atattcgtgt tcgtcctgga cttcccggtg ccgcttcccc
     4141 gcgcggtccg catcgtgggc ggcatcctcg cgacggccgc cgccgccctc gcgctctggc
     4201 gcatcgtgtt cacgcccgac tacatcctcg ccacggtccg cgccggatcg gacatagtgc
     4261 gcgtcgacgg cccgcagtac ggcctcatca acaacctgga gacgggcctg gcggccctct
     4321 ccgcggccat actcctcgcc cggagccttg gtattaagag ccggatccag aggcagcgcg
     4381 gcctcgtggc cttcgccggc gtggtcgtcg gcgccggctt catctggctc ctgccccagc
     4441 tcgtcgtctc gcgctcggtc gtccgctcgg tcttcgccct caccccggtc gccgccctgg
     4501 tgctcgcctc ggtcgtcacc tacgccttct acctctccag aatcttcgac tggcgctcga
     4561 tcgggcgcca gctcgcctac tacgccctcc tcaccctcgt ggtcggcctg cccacgggtt
     4621 tcgtggtcta cgtcctcacc ctcctccgca tcgtgtcgga gacggtgccc ctcgtcggca
     4681 ccttcctcgt gttcttcgtc tcgctctacc tcgcccgcgg cttctcggcc cgcttcctcg
     4741 cccgctcggg ggccccgggc gagtaccgcg aggagctcga gagcggcctt gcccacatcg
     4801 acctcgccga gggccgcgac ctggtcctcg ggaagctgta caagctcctc tcggccgccc
     4861 tcgacttctc cgacttcacc gtcctcatcg aggacgacca gggcagcctc aagagcgtct
     4921 tctcgcccca gggctccaag gccctcatcg agaagggctc ggacctggcc gtcgccctcg
     4981 accgctccgg cgccaccgtc ctcctcaagt ccgaggccct cgcccacccc gcctacgagg
     5041 acgtgcgggc cgagttcatg agcctgttcg agagcctcaa cgccgaggcc ttcatcctcg
     5101 cccgggaggg ccgccacatc atcggggcct tcatcctcgg cgcgcggcgc accggagccg
     5161 acttcaccga ctacgactac gacaccttca agtcgatcta cggcaagctc ttcgtcttcg
     5221 cctactacct caagaacgtc gccagggaat ccctgctcta cacggtcgac cgcgagatcg
     5281 ccctctccga ccagatcatc cgcttcgcgc tggagaaggt ggacaccttc cggcatccca
     5341 aggtggacgc ggcctgggcc ctgcgctaca tgcgcaccct cggcggcgac ttcatcgact
     5401 tcgtccgcct ttcccccgac cgctggttct tcgtcatggg cgacgtctcg gggaagggcc
     5461 tctcggcctc gatgaacatg ctcatcctga agtccatgat caggaccttt ctcagggtcg
     5521 agaaggactt cgtgtctctc gtgtccagga tcaacagctt catcaaggag aacctgcccc
     5581 gcggcaactt cttcgcgggg atcttcggct acttcgacct ggcgaaggac gccttctact
     5641 acatcaactg cggcattccc gccctcctcc tctactcccc cagcttcgac gccttcatcg
     5701 aggtccaggg cgagggcaag atcctcggct tcgtccgcga catcaagccc tacctcaagc
     5761 cccgcaagct cgtcctgccc ccgggctgcg tgctcgtggc cgcgaccgac ggcatcaccg
     5821 acagcgagaa cctcaggggc gagcgcttcg gcaaggagcg gctgcggcgc tcgatccagg
     5881 accgtctggg ccaggcctcg aacgagatcg tcggcggcgt cctcgacgac ctcctctcct
     5941 tctcggaccg gaaacaggac gacgacctca ccctcttcgt gatcaaattc cccccaagga
     6001 gcgccgaatg acccagctcg atatcacaac caggaacgag gaagggcacg agatcctcgt
     6061 ggtgtccggg cccatcaaca gctacacctt caccgagttt caggagaagg tgtacaaggc
     6121 gatccgcgcc ggcgacacga tcatcgacat ggagaaggtg acgaccctct cctcggccgg
     6181 catcggggtg ctgatggccg ccctcgacga cggcgaggcc gagggccaca ggctcgtcgt
     6241 cctcaagccc tcggagatcg tccgcctggc catggactcg acgggcttcg ccgaccgctt
     6301 ccccgtgata cagacgctgc gggagttctg atgagggacg agggggaggg gccggggccg
     6361 cctgggaagt ccagagcgcg gcaggcttcc cgggcgacga agatactcgt cgcgtctgcc
     6421 gaggccgtga tggtccttga gggattcgtc gacggcctgg acttcctggc gcggcgggag
     6481 cgccacagga taaggctcgc cggcggcgag atcctcgaca acctggtgcg ccactcagcc
     6541 cccctggccg gaggcagcct ctcggtgagg gtctcgcggc ggaggacggg gatcgtcatg
     6601 gccttcttct tccgctcccc caaattcgcc gccttcgctg cccgctgctc cgatcccgag
     6661 cccctcttcg accccgccca ccggcgctgg cgcggcatcg gtatggtgat gtgcaggaac
     6721 ctctcgagct cgatgaggct caggcccggc cgtttcatcg acaggatctt cctcagcttc
     6781 cgcctcgagc ccgacgccca caccgatccc gcgggcagac ccgggatgga gcttggcccg
     6841 gaaggccccg gccagagtac tggccgcgcc cggctcgccg agaaataact gcaccatact
     6901 ggcaatatct tcccaagcca ggggcccctt ccggcgt
//
LOCUS       DASSQV010000011         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472 k141_3293,
            whole genome shotgun sequence.
ACCESSION   DASSQV010000011 DASSQV010000000
VERSION     DASSQV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3293"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFL04_00205"
     CDS             complement(<1..243)
                     /locus_tag="VFL04_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET7838147.1"
                     /translation="MTAREQDILRRFIVLEGVDGAGTTTQLANIDEALSRAGLPHWKT
                     AEPTARPEGLLIRSILGGELGADPGTVAHLFAADRHE"
     gene            339..>2527
                     /locus_tag="VFL04_00210"
     CDS             339..>2527
                     /locus_tag="VFL04_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838148.1"
                     /translation="MSGTKMFSVRLSPPLAAAIILFALAAFLVAPDLDKGIRGGMQKA
                     LGLAKVEAEDAIGLDISFESLSPSMLSSLTISNLALRNPRGGTVLEAKRVDVYYNLWS
                     VISRRGPEAISEIRLEGASIDLQLPADRAMLEDLARRLGITEAAALPPILITGRDVGL
                     RVAIEGSGSYEIKADSLSYSTRGAEIAVQAKGRAVADTRGLAGRVEGQVELSGMLAKD
                     FERARFSLSVSASSPDFQLSTQRFELVYAGGVAELLKVRDKAPLDASLRYDLRDGSLT
                     ASLRLDNYPLRRSIRLSGALAALRPWLESPYSGNLGFSLPGGDVSRISYDIDLDGTLP
                     PNILPRRYRTSLLAHGDSRSIIVERARLEEGDQAFEYRGSLQFRDLAPDGELEVRFSL
                     LDGRLPISSRFKVLGHGGEFAALSEGLTVGGVGIEDLAVSAAYRRRQVEFGLSFRPPE
                     AEEPGATLPSPRFSGEPGSQLGALPRVFCEGSLNLEGKPSLDVAVDLEELDLNPLRPI
                     LEALSPSRELTGFVSQLKLGGALYLATDFEALSWSASDLAVVSRSSPGAYALLSLSGN
                     LESIEVRKATLALGGYTIEGRASLDLAGHGRVGFETSFKLKDIPYQLRGSLVENGIFI
                     SGDYGLEVSARSSDGETFVKARARDLPFPVGGGVVLATLDADGRFSSAKDWRLALSVL
                     SVVPAGERLGRLPTVSLSGVIDQDSGDIPQISVGDRTSTLVGSMSFG"
BASE COUNT          362 a          912 c          833 g          420 t
ORIGIN      
        1 ctcgtggcgg tcggcggcga agaggtgggc gaccgtgccc ggatcagccc cgagttcgcc
       61 cccaaggatg gacctgatga ggaggccctc gggcctcgcc gtcggctcgg cggtcttcca
      121 gtgcggcagt ccggcccggg agagcgcctc gtctatgttc gcgagctggg tcgtcgtgcc
      181 ggccccgtcg accccctcaa ggacgatgaa gcggcggagg atgtcttgtt cccttgcagt
      241 catgcaggga gagtagcggg gaagacgatc cctatcaaga aggcgggccc gtctttgtcg
      301 atgccgagtg atgaccgccg gcgcgctatt gtggtatcgt gagcggcacg aagatgttca
      361 gcgtccgact ctccccgccc ctcgccgcgg cgatcatcct ttttgccctc gcggccttcc
      421 ttgtcgcccc cgatctggac aagggcatca ggggaggcat gcagaaggcc ctcggccttg
      481 ccaaggtcga ggccgaggac gcgatcggcc tcgacatctc cttcgagtcc ctctccccct
      541 cgatgctaag ctccctcacg atcagcaacc tggcgctgag gaacccgcgg ggagggacgg
      601 tcctcgaggc caagcgggtc gacgtctact acaacctgtg gtcggtgatc tcgcgcaggg
      661 ggccggaggc gatctcggag atcaggctcg agggggcctc gatcgatctc cagctgcccg
      721 ccgacagggc catgctcgag gaccttgccc gccgcctcgg gatcaccgag gccgccgccc
      781 tgccccccat cctcatcacg gggcgggacg tcgggctcag ggtggccata gagggctcgg
      841 gcagctacga gatcaaggca gacagcctca gctactccac ccgcggcgcg gagatcgccg
      901 tccaggcgaa ggggagggcg gtggccgaca cgagggggct cgcgggccgg gtcgagggcc
      961 aggttgagct gagcgggatg ctcgccaagg actttgagcg ggccaggttc tcgctctcgg
     1021 tgtcggccag ctcccccgac ttccagctgt cgacccagcg tttcgagctc gtctacgccg
     1081 ggggcgtggc cgaactcctc aaggtcaggg acaaggcccc cctcgacgcc tccctccgct
     1141 acgacctcag ggacgggagc ctgacggcct cgctcagact tgacaactac cccctccggc
     1201 ggagcatcag gctttccgga gccctcgccg ccctccgccc ctggctcgag tccccctata
     1261 gcggcaacct cggcttctcc ctccccgggg gcgatgtctc gaggatctcc tacgacatcg
     1321 acctggacgg gaccctccct ccgaacatcc tgcccaggcg ctaccggacc tccctcctgg
     1381 cccacgggga ctcgaggtcc atcatcgtcg agcgcgccag gcttgaggag ggcgaccagg
     1441 ccttcgagta caggggatcg ctccagttca gggacctggc ccccgacggc gagctcgagg
     1501 tccgcttcag cctcctcgac ggcaggcttc cgatatcgtc caggttcaag gtccttggac
     1561 atggcgggga gttcgcggcc ctctccgagg gcctcacggt gggcggggtg ggcatcgagg
     1621 acctcgccgt ctccgcggcc taccggaggc gccaggtcga gttcggcctc tccttccgcc
     1681 cgcccgaggc cgaggagccg ggggccaccc tccccagccc gaggttctcc ggcgagccgg
     1741 gatcccagct cggggccctt ccgcgggtct tctgcgaggg cagcctcaat cttgagggca
     1801 agccctccct cgacgtggcg gtggacctgg aggagctgga cctcaatccc ctccgcccta
     1861 tcctcgaggc cctcagcccc tcgcgcgagc tcacgggctt cgtgtcccag ctcaagctcg
     1921 gcggagccct ctacctcgcc accgatttcg aggccctctc ctggtcggcc tcggatctgg
     1981 ccgtggtctc ccgcagctcg cccggcgcct atgccctgct ctcgctctcg ggcaacctgg
     2041 agtcgatcga ggtaaggaag gccaccctcg ccctgggcgg ctacacgatc gagggcaggg
     2101 caagcctcga cctggccgga cacgggcggg tgggcttcga gacgagcttc aagctcaagg
     2161 acatccccta ccagctccgg ggctccctgg tcgagaacgg gatcttcatc tcgggcgact
     2221 acggccttga ggtctcggcc cggagctccg acggcgagac cttcgtgaag gcccgcgccc
     2281 gcgacctgcc tttccccgtt gggggagggg tcgtcctggc caccctcgac gccgacgggc
     2341 gcttctcctc ggcgaaggac tggaggctcg ccctctccgt cctgtccgtc gtcccggcgg
     2401 gcgagcgcct cggccgtctg cccacggtga gcctctctgg cgtcatcgac caggattccg
     2461 gcgacatccc gcagatcagc gtcggcgacc ggacctcgac cctcgtgggg tccatgagct
     2521 tcggcta
//
LOCUS       DASSQV010000012         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2241696, whole genome shotgun sequence.
ACCESSION   DASSQV010000012 DASSQV010000000
VERSION     DASSQV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241696"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3314
                     /locus_tag="VFL04_00215"
     CDS             <1..3314
                     /locus_tag="VFL04_00215"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7838149.1"
                     /translation="RDPAAEPPHEVMAPVSETENRVVENAGEDGPWFLSAEVQDIAGN
                     WSAPMRISYVRKLTPPRPPLVLHPDLDDKGFLASNTFTVRWTSPEKEPIAGYTWELRY
                     AGALETAGVSAGLKAPGAAAKPPLPGLDAYESALVANAGPPLPPRRILGSSPSASWDD
                     VDDGYYLLSVAAVDDVGNISDASTILLRANKYVPYTLVTYSDPSRDDLGKTSLSIIGK
                     GFAADGDITKVVLDRDGKEPYDIERDLADGGFRIRSDRQIDGFGFEDAEAGSYRIGLF
                     HPKRGWYWTGPKVAIDVSGTIKYGVETAYVPSWIPVAPRVFRFSIYDAVILVAVLFGG
                     LGVVLSSGQAIAAVRDGFAVRLQVQALVYGGPRPSAAKTRKERKLGRRGLGLRVKFTL
                     FIALLALFVVFLVAVPLGVSVTRAESASLAQGLQQRALVLLESVAQGARTYLPNQDTL
                     QLGFLPQQKQAMDDALYVTVTGYAKKATDPDAVWASNDPGLAAKIDTSGLDPGTSRIK
                     DPLSPKIAAIAAEIDAAAAAQVGDINKSITDLNREAIGLATRIATDEAAKRRFEEISQ
                     TVTRLRSALNDRLFAIASRSVGSVPAFDPGALELKPRTYVFYKPVLYVQTNDQLYYRG
                     MVRIEVSTDRIVADVVRAQANVIRITLIIAAVALGLGIAGAFLLSLIIVVPISKLVKQ
                     IEHIRDTPDKEDLAGERIEVASRDELYDLAETVNQMTEGLVKAAKASKELIVGKGIQK
                     MFIPLDASSSRAKLSTGRRDEKSFEVFGYYEGAKGVSGDYWDFRSINSRYHYFIKCDI
                     SGKGVSAALIMVEVATMVINYFNEWKKAMPRNIDLTGIAYRINDFIEERGYEGKRFAA
                     FTLGLWDSEEGKAYLCEAGDRKLHVWDARAQRVVEELLPDSPAAGIFPSFMVQMKTPF
                     VQVPRKLESGDILLLYTDGIEEAKRHFRDRDFRVISCEDAEKEKPHGNHTGGQDNEEF
                     GYERLTAVLEAVNARGSYRLEKAHDPVPQGALSFDFSTCTGGLEEKIIALVSVEKVFR
                     MYPDPQATDQDVVLVDEKIDSFLEKHFDQYRLYCSKKRPHLDPQHENPGYLLYAGIKE
                     DDQYDDLTSLGIMRK"
BASE COUNT          624 a         1271 c         1044 g          507 t
ORIGIN      
        1 gcagggaccc cgccgccgag ccgccgcacg aggtcatggc cccagtctcc gagaccgaga
       61 accgcgtcgt cgagaacgcc ggcgaggacg ggccatggtt cctctcggcc gaggtccagg
      121 acatcgccgg caactggtcg gccccgatgc ggatcagcta tgtgcggaag ctcacgcccc
      181 cgcggccgcc cctcgtcctc catcccgacc ttgacgacaa gggcttcctc gcctcgaaca
      241 ccttcaccgt gcgctggacc tcgcccgaga aggagcccat agccggctac acctgggagc
      301 tccgctacgc gggggcactc gagacggccg gggtctcggc gggcctcaag gccccgggcg
      361 ccgcggccaa gccgcccctg ccaggcctcg acgcctacga gtcggccctc gtcgcgaacg
      421 ccggcccacc cctgcctccg cgccgcatcc tcggcagttc gccttcagcc tcgtgggacg
      481 acgtggacga cggctactac ctcctctcgg tggccgcggt ggacgacgtc ggcaacatca
      541 gcgacgcctc gacgatcctc ctgcgcgcca acaagtacgt gccctacacc ctggtcacct
      601 actccgaccc gagccgcgac gacctcggca agacttcctt gagcatcatc ggcaagggct
      661 tcgccgccga cggcgacatc acgaaggtgg tgctcgaccg cgacggcaag gagccctacg
      721 acatcgagcg cgacctcgcc gacggcggct tcaggatccg ctcggaccgg cagatcgacg
      781 gcttcggctt cgaggacgcc gaggccggca gctaccgcat cggtctcttc catcccaagc
      841 ggggctggta ctggacgggg ccgaaggtcg ccatcgacgt ctccggcacg atcaagtacg
      901 gggtcgagac ggcctacgtc ccctcatgga tccccgtcgc cccccgcgtc ttccgcttct
      961 cgatctacga cgccgtcatc ctcgtggcgg tcctcttcgg aggcctcggc gtcgtcctct
     1021 cctcgggtca ggccatagcc gccgtcaggg acggcttcgc ggtcaggctc caggtccaag
     1081 cactcgtcta tggaggtccc aggccctcag ccgccaagac caggaaggag cggaagctgg
     1141 gccgcagagg cctcggtctc cgggtcaagt tcaccctctt catcgccctc ctggccctct
     1201 tcgtcgtctt cctcgtcgcc gtccccctcg gggtctcggt cacgagggcc gagagcgcct
     1261 cgctcgccca gggcctccag cagcgcgccc tcgtccttct cgagagcgtg gcccagggcg
     1321 ccaggaccta tctgcccaac caggacaccc tccagctcgg cttcctcccc cagcagaaac
     1381 aggccatgga cgacgccctc tacgtcaccg tcaccggcta cgcgaagaag gccaccgacc
     1441 ccgacgcggt ctgggcctcc aacgaccccg gcctcgccgc gaagatcgac acctcgggtc
     1501 tggatccggg gacgagcagg atcaaggacc ccctgagccc caagatcgcc gccatcgccg
     1561 ccgagatcga cgcggccgcc gccgcccagg tgggggacat caacaagtcg atcaccgacc
     1621 tcaaccgcga ggccatcggc ctggccacga ggatcgccac cgacgaggcc gccaaacgcc
     1681 gcttcgagga gatctcgcag acggtcacga ggctccgcag cgccctgaac gacaggctct
     1741 tcgccatcgc atcgaggtcg gtcggctcgg tgccggcctt cgaccccggg gccctcgagc
     1801 tcaaacccag gacctatgtc ttctacaagc cggtgctcta cgtccagacc aacgaccagc
     1861 tctactaccg gggcatggtc aggatcgagg tcagcaccga caggatcgtc gccgacgtcg
     1921 tcagggccca ggccaacgtg atccgcatca ccctcatcat cgcggccgtg gccctcgggc
     1981 tcggcatcgc gggagccttc ctcctgtccc tgatcatcgt ggtgcccatc tcgaagctcg
     2041 tgaagcagat cgagcacatc cgcgacaccc ccgacaagga ggacctcgcc ggtgagagga
     2101 tcgaggtcgc gagccgggac gagctctacg acctcgccga gacggtcaac cagatgaccg
     2161 agggcctcgt gaaggctgcc aaggccagca aggagctcat cgtcggcaag ggcatccaga
     2221 agatgttcat ccccctcgac gcgagctcct cgcgcgccaa gctcagcacc ggacggcgcg
     2281 acgagaagag cttcgaggtc ttcggctact acgagggcgc gaagggagtc tcgggcgact
     2341 attgggactt caggtccatc aattcccgct accactactt catcaagtgc gacatctcgg
     2401 gcaagggcgt ctcggcggcc ctcatcatgg tcgaggtcgc gaccatggtc atcaactact
     2461 tcaacgagtg gaagaaggcg atgccgcgca acatcgacct cacgggcata gcctacagaa
     2521 tcaacgactt catcgaggag cgcggctacg agggcaagcg tttcgccgcc ttcacactgg
     2581 ggctctggga ctccgaggag ggcaaggcct acctctgcga ggccggagac cgcaagctcc
     2641 acgtctggga cgccagggcc cagcgcgtcg tcgaggagct cctgcccgac tcccccgccg
     2701 ccggcatctt cccgagcttc atggtccaga tgaagacccc cttcgtgcag gtgccccgca
     2761 agctcgagag cggggacatc ctcctgctct acaccgacgg catcgaggag gccaagcggc
     2821 atttccgcga ccgcgacttc agggtgatca gctgcgagga cgccgagaag gagaagcccc
     2881 acggcaacca caccgggggc caggacaacg aggagttcgg ctacgagcgc ctcaccgccg
     2941 tcctcgaggc cgtgaacgcc agggggagct accgcctcga gaaggcccac gaccccgtgc
     3001 cccagggggc cctgagcttc gacttctcga cctgcaccgg gggcctcgag gagaagatca
     3061 tcgccctcgt gtcggtggag aaggtcttcc gcatgtaccc cgacccccag gccaccgacc
     3121 aggacgtcgt cctcgtggac gagaagatcg acagcttcct ggagaagcac ttcgaccagt
     3181 accggctcta ctgctcgaag aagcgtcccc acctcgaccc ccagcacgag aaccccggct
     3241 acctcctcta cgcgggcatc aaggaggacg accagtacga cgacctgacg agcctgggca
     3301 tcatgaggaa atgagggatg agggcgtggg ccagaccctg gtcggaacct gcggctactc
     3361 ctacgacgag tggcgcgggg tcctctatcc gcccgagctg ccgaaggccg agttcctccc
     3421 cttctacgcc gggaccttcc ccttcg
//
LOCUS       DASSQV010000013         4472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_874554, whole genome shotgun sequence.
ACCESSION   DASSQV010000013 DASSQV010000000
VERSION     DASSQV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874554"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1234
                     /locus_tag="VFL04_00220"
     CDS             119..1234
                     /locus_tag="VFL04_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET7838150.1"
                     /translation="MIDQRLKKLASILVNYSCKVQKGEKVLIQNTNLEVPFVNAIIDA
                     VHDAGGLPFISIRDRVLDRALYSRASVEQFELQARFEAERMKEMDCFIGFTSLRNQFA
                     WADLPQDKLDLYNGIIGKRVHMDVRLPDTRWVVLRFPSPAFAQLASMSEEAFEDWYFS
                     VCTLDYSKMSQAMDSLVSVMAKTDKVRIVGPGTDLSFSIKGLPPVKCDGSMNIPDGEV
                     YTAPVRDSVNGVISYNAPAEREGFTFEKIVFEFDRGRIVKASANDTARVNRVLDIDAG
                     ARYVGEFAIGVNPFITKPMKETLFDEKIAGSIHFTPGNSYDDCFNGNRSALHWDLVLI
                     QTPEWGGGQIWFDGRLIREDGRFVVPELAGLNPERLA"
     gene            1460..1744
                     /locus_tag="VFL04_00225"
     CDS             1460..1744
                     /locus_tag="VFL04_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HET7838151.1"
                     /translation="MEKRLGIVAIVIEESSSVPRVNALLSDFSRLVDARLGLPMRGKG
                     ISLISLVVEGDTDDIGALTGRLGKIPGVQVKSVLTAYREAEDGRVEEQLH"
     gene            1716..3146
                     /gene="hydG"
                     /locus_tag="VFL04_00230"
     CDS             1716..3146
                     /gene="hydG"
                     /locus_tag="VFL04_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HET7838152.1"
                     /translation="MDGSKSSFIDRERIGNVLAESRPDGDAVRRTIDKALELKGLGPE
                     EAALLLNAEEPELEAELLAAAGKVKELIYGRRLVLFAPLYIGNRCSNDCTYCAFRASN
                     RDLARRDLSLDEVELEVRALLREGHKRLLVLAGEDASETDEVTAARWVEAIRRIYSVR
                     EPLPRGVGEASIRRVNVEIAPLGVEGFRLLKTANIGTYACFQETYDPQLYKDFHPRGP
                     KADFVWRLEVMDRAMEAGIEDVGIGALFGLGDFRFEVLGMLEHARHLEARFGCGPHTV
                     SVPRIEPAPGAPDAMAVPHAVSDREFRRIVATLRLALPYTGIIMSTREREELRDELFR
                     YGVSQISAGSRTNPGAYSEDGAKAVEAGSQFQLGDHRSLEEVVTKLAEMGYVPSFCTG
                     CYRKGRVGADFMDLAKPGLIKRFCEPNALFTFAEYVKDFAGPAMAGPAASLKASLLAT
                     LPDEETRKRSIEALARVEAGERDIYF"
     gene            3156..4169
                     /gene="hydE"
                     /locus_tag="VFL04_00235"
     CDS             3156..4169
                     /gene="hydE"
                     /locus_tag="VFL04_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HET7838153.1"
                     /translation="MDNSQRDMVIWYLNASTEELGLRAREVCEREHGKKVLLRGLIEF
                     SNTCALNCHYCGIRAGNPGVERYRLKPDELVGAAAGAYAAGLRSFVLQSAEDPWYSTD
                     RLAAVLQRIKAACPEAAITLSCGVKPRSEYRELKAAGADRYLLRFETSDPELHRRLRD
                     GLTLEARLKALEDLRAEGYQVGSGFMVGLPGESEKTGIDNALLCAELGLDMVGIGPFI
                     PHPATPLAGSPQLGLERTLRLTSLLRLLMPRVHLPATTAAGSLDPEGREKMIMAGANV
                     LMPNMTPVPVRKNYLLYPGKICLDEAGEKCLGCLRLRMKTVDRELSFARGDSLRRGEG
                     HVA"
     gene            4159..>4472
                     /locus_tag="VFL04_00240"
     CDS             4159..>4472
                     /locus_tag="VFL04_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET7838154.1"
                     /translation="MSPEPLYGQETAKALENFGPGRTPRELIAAYAKVKRACVAAVQE
                     VEGRFEPELFACLVEALEEIAAGRHDSQFPLPLRQGGAGTSFNMNLNEVAAARTRALY
                     KER"
BASE COUNT          759 a         1591 c         1440 g          682 t
ORIGIN      
        1 gccgggcgac tcgttctatg ccgggccggg ccagaggcac ggcgcgacct gcctttcggc
       61 cggccgactc ctccacgtct tcagccccca gcgccccgac tacctctaag gagcccccat
      121 gatcgaccag cgtctcaaga aactcgcgtc catactcgtc aactattcct gcaaggtcca
      181 gaagggcgag aaggtcctca tccagaacac gaacctcgag gtgcccttcg tgaacgccat
      241 catcgacgcc gtccacgacg ccggcggcct gcccttcatc tcgatccgcg accgggtcct
      301 cgaccgcgcc ctgtactcca gggccagcgt cgagcagttc gagctccagg cccgcttcga
      361 ggccgagcgc atgaaggaga tggactgctt catcggcttc acctcgctgc gcaaccagtt
      421 cgcctgggcc gacctgcccc aggacaagct cgacctctac aacggcatca tcggcaagcg
      481 ggtgcacatg gatgtgcggc tccccgacac ccgctgggtg gtcctccgct tcccctcccc
      541 ggccttcgcc cagctcgcct cgatgagcga ggaggccttc gaggactggt acttctcggt
      601 ctgcaccctc gactactcga agatgtccca ggcgatggac agcctcgtct cggtcatggc
      661 gaagaccgac aaggtccgca tcgtgggccc ggggaccgac ctgagcttct cgatcaaggg
      721 cctgcccccg gtcaagtgcg acgggtccat gaacatcccc gacggcgagg tctacaccgc
      781 cccggtgcgc gattcggtca acggcgtgat ttcgtacaac gcgcccgccg agcgcgaggg
      841 cttcaccttc gagaagatcg tcttcgagtt cgaccgcggc aggatcgtga aggcgagcgc
      901 caacgacacg gcccgggtga accgggtcct ggacatcgac gcgggcgccc gctacgtcgg
      961 cgagttcgcg atcggcgtga accccttcat caccaagccc atgaaggaga ccctctttga
     1021 cgagaagatc gcgggctcga tccacttcac cccgggcaac tcctacgacg actgcttcaa
     1081 cggcaaccgc tcggccctcc actgggacct cgtcctcatc cagaccccgg agtggggcgg
     1141 cgggcagatc tggttcgacg gcaggctcat ccgcgaggac ggccgtttcg tcgtgcccga
     1201 gctcgccggc ctgaatcccg agcggctcgc ctagggctgg agccgacccg atcgggcccg
     1261 ggcagggccc cttcgggtcg gagctcccct tgctgagagg accgaccctg tcgggtcggc
     1321 cttgacaaac gataaaaata gatccattat gccgctgaca ctgagagacg gatttcatcc
     1381 tgctcgaggt gcgcctgaca gccgcgtgat cggcctcgtt cgaggcccgc ggccaatccg
     1441 atccggaggg aacagcctga tggagaagag acttggcata gtcgccatcg tgatcgagga
     1501 gtcctcctcg gtaccccgcg tcaacgccct gctctccgat ttctcgcgcc tcgtcgacgc
     1561 ccgcctgggc ctcccgatgc gcggcaaggg catttccctc atctccctcg tcgtcgaggg
     1621 tgacacggat gacatcggcg ccctgaccgg caggctcggg aagatcccgg gcgtacaggt
     1681 gaagtccgtc ctcaccgcat acagggaggc cgaggatgga cgggtcgaag agcagcttca
     1741 ttgatcgcga gaggataggg aacgtcttag ccgagtcccg tcccgacggc gacgcggtca
     1801 ggagaaccat agacaaggcg ctggagctca agggcctcgg acccgaggag gccgcgcttc
     1861 tgctgaacgc cgaggagccc gagctcgagg ccgagctcct ggccgccgcg ggcaaggtga
     1921 aggagctcat ctacgggcgc cgcctcgtgc tcttcgcccc cctgtacata ggcaacaggt
     1981 gctcgaacga ctgtacctac tgcgccttca gggcgagcaa ccgcgatcta gcccggcgcg
     2041 acctcagcct ggatgaggtg gagctcgagg tgcgggccct cctccgcgag ggccacaaac
     2101 gcctcctcgt cctcgccgga gaggacgcca gcgagaccga cgaggtcacg gcggcgcgct
     2161 gggtcgaggc catcaggcgg atctactcgg tcagggagcc cctgcccagg ggcgtcggcg
     2221 aggcctcgat caggcgggtg aacgtggaga tcgcccccct gggcgtcgag ggcttccgcc
     2281 tcctcaagac ggccaacatc ggcacctacg cctgtttcca ggagacctac gacccccagc
     2341 tgtacaagga ctttcacccc cgcggcccca aggccgactt cgtgtggcgt ctcgaggtca
     2401 tggacagggc gatggaggcc ggaatcgagg acgtgggcat cggcgccctc tttgggctgg
     2461 gcgacttccg cttcgaggta ctcggcatgc tcgagcacgc gaggcatctc gaggcccgct
     2521 tcggctgcgg cccgcacacg gtcagcgtgc cccgcatcga gccggccccg ggcgcccccg
     2581 acgcgatggc cgtgccccat gcggtgagcg accgcgagtt ccgccgcatc gtcgccaccc
     2641 tccgcctcgc cctgccctac accggcatca tcatgagcac ccgcgagcgg gaggagctgc
     2701 gcgacgagct cttccgctac ggggtgagcc agatctctgc gggctcgcgc acgaaccccg
     2761 gggcctattc cgaggacggc gccaaggccg tggaggcggg cagccaattc cagctcggcg
     2821 accacaggag cctcgaggag gtcgtgacga agctcgccga gatgggctat gtgccctcct
     2881 tctgcacggg ctgctaccgc aagggcaggg tcggggccga cttcatggac ctcgccaagc
     2941 ccggcctcat caagcgcttc tgcgagccca acgccctgtt caccttcgcc gagtacgtga
     3001 aggacttcgc cggcccggcc atggcaggcc ccgcggcgag cctcaaggcg agcctcctcg
     3061 ccacgcttcc ggacgaggag accagaaaga ggtcgatcga ggccctcgcc cgcgtcgagg
     3121 cgggcgagcg ggacatctac ttctaggcgg cggtcatgga caacagccag cgcgacatgg
     3181 tcatctggta cctgaatgcc tccaccgaag agctcggcct ccgggcccga gaggtctgcg
     3241 agcgggagca cgggaagaag gtcctccttc gcggtctcat cgagttctcg aacacctgcg
     3301 ccctcaactg ccactactgc gggatcaggg ccggcaaccc gggcgttgag cgctacaggc
     3361 tcaagcccga cgagctcgtc ggcgcggcgg ccggggccta cgccgccggc ctgcgaagct
     3421 tcgtcctgca gagcgccgag gatccctggt acagcaccga caggctggcc gccgtgctgc
     3481 agcggatcaa ggcagcctgt cccgaggcgg ccatcaccct gagctgcggc gtcaagccgc
     3541 gctccgagta ccgcgaactc aaggccgcgg gggccgaccg ctacctcctc cgcttcgaga
     3601 cctcggaccc tgagctccac cgccgtctgc gcgacggcct taccctcgag gcgaggctca
     3661 aggccctcga ggacctgcgc gccgagggct atcaggtggg ctcgggcttc atggtgggcc
     3721 tcccgggaga gagcgagaag acagggatcg acaacgccct gctctgcgcc gagctcggcc
     3781 tcgacatggt ggggatcggc cccttcatcc cccatccggc cacgccgctg gcaggctcgc
     3841 cccagctggg cctcgagcgg accctccgcc tcacgagcct ccttcggctc ctcatgccca
     3901 gggtccacct ccccgccacg acggcagccg gctccctcga tcccgagggc cgcgagaaga
     3961 tgatcatggc aggggcgaac gtgctcatgc cgaacatgac ccccgtgccg gtgcgcaaga
     4021 actacctcct ctatcccggg aagatctgtc tcgacgaggc cggggagaag tgcctcggct
     4081 gtctccgtct gcgcatgaag accgtggaca gggagctcag cttcgcccgc ggggattccc
     4141 tcagacgcgg tgagggccat gtcgcctgag cccctctacg ggcaggagac ggccaaggcc
     4201 ctcgagaact tcggcccggg gaggacgcct cgcgagctca tcgccgccta cgccaaggtg
     4261 aagcgggcct gcgtggccgc agtccaggag gtggaggggc gtttcgagcc cgagctcttc
     4321 gcctgtctgg tcgaggccct tgaggagatc gcggcgggcc gccacgactc ccagttcccc
     4381 ctgcccctcc ggcagggcgg ggcggggacg agcttcaaca tgaacctgaa cgaggtggcc
     4441 gccgcccgca cccgcgccct gtacaaggaa cg
//
LOCUS       DASSQV010000014         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_377647, whole genome shotgun sequence.
ACCESSION   DASSQV010000014 DASSQV010000000
VERSION     DASSQV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377647"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /gene="mraY"
                     /locus_tag="VFL04_00245"
     CDS             <1..968
                     /gene="mraY"
                     /locus_tag="VFL04_00245"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET7838155.1"
                     /translation="FGPKIIERLRVLKFGQSVRTDGPESHLAKSGTPTMGGILLILAV
                     SVGVLLWADLKNLYVWVSLGALLGFGLVGLADDWLKIKKRNSDGIPAWVKFWGQVIVA
                     SGAALALYFTGHSEVTRLYVPFFKFPVIDLGVVWIPIAVIYVVYWSNAVNLADGLDGL
                     ATGLVIMALIAFSVLTYITGRQDWAAYLNIPYIKGAGELTVFNLALIGACVGFLWFNS
                     HPAEVFMGDVGSLALGGVLGVLSLIVKKEILLLLVGGVFMMEIISDVLQVGYFKLTKG
                     KRLFKMAPLHHHFELKGWKETKVVTRFWILGGLFAIVALSTLKIQ"
     gene            976..2151
                     /gene="ftsW"
                     /locus_tag="VFL04_00250"
     CDS             976..2151
                     /gene="ftsW"
                     /locus_tag="VFL04_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET7838156.1"
                     /translation="MKAGFALERMRAGAPGDRGILVAIAVLTGTGIAALWSASSDYAL
                     SLGKSAGYFAARQAIYLLPAGLVFALCATVDLDSIRKRTGLITLATLLVLLLPFVPGL
                     GENRNGATRWIDLGVTTFQPSELWKLATVLYLAHILDKKADRISESPGVLIPPFLLVA
                     LGCIVVYLQTDFSTAVVIGVAAAAVFWASGAPVSFFLGLGAAAAPLAALSVLTSDFRL
                     RRILTFMFPAYEPHGQGYQVLGSIRAIRSGGLVGKGLGLGTLKQGSIPEVHSDFVFSA
                     WTEETGLVGVLVVLALWAFIAWRGFKVALGETDRYRSSLVCGLTFLLCIEVLVNLGVV
                     SGAVPATGMALPFFSAGGSSLLSTAVACGLIYNASRSPANARARGPNALAAGEVGDV"
BASE COUNT          296 a          837 c          721 g          443 t
ORIGIN      
        1 ttttcggccc caagatcatc gagcggctcc gtgtgctcaa attcggccag tccgtgcgga
       61 ccgacggccc cgagagccac ctcgccaaat cggggactcc cacgatgggc ggcatcctcc
      121 tcatcctcgc cgtctccgtc ggggtcctcc tgtgggccga cctcaagaat ctgtacgtct
      181 gggtgtccct cggggccctc ctcggcttcg gtctcgtggg cctcgcggac gactggctca
      241 agatcaagaa gcgcaactcc gacggcatcc cggcctgggt caagttctgg gggcaggtga
      301 tcgtggcctc gggcgcggcc ctcgccctgt atttcacggg gcacagcgag gtgacccggc
      361 tgtacgtgcc gtttttcaaa tttccggtta tcgacctcgg cgttgtgtgg atccccatcg
      421 cggtgatcta cgtggtgtac tggtccaacg cggtcaacct cgccgacggg ctcgacgggc
      481 tcgccacggg gctcgtcatc atggccctca tcgccttctc cgtcctgacc tacatcacgg
      541 gcaggcagga ctgggccgcc taccttaaca tcccgtacat caagggcgcg ggcgagctca
      601 cggtcttcaa cctcgccctc atcggagcct gtgtgggctt cctctggttc aattcccatc
      661 cggccgaggt cttcatgggc gacgtgggca gtctggccct cggcggcgtc ctcggcgtgc
      721 tgtcgctcat cgtgaagaag gagatcctcc tcctcctcgt cggaggcgtc ttcatgatgg
      781 agatcatctc ggacgtcctc caggtcgggt atttcaagct gacgaagggc aagcgcctct
      841 tcaagatggc gcccctgcac caccatttcg agctcaaggg atggaaggag acgaaggtcg
      901 tcacgcggtt ctggatcctc ggcggccttt tcgcgatcgt ggctctttcc accctgaaga
      961 tacagtgagg cgggcatgaa ggcgggattc gcgctggagc gcatgcgggc gggagcgccc
     1021 ggggatcgcg gcatactcgt cgcgatcgcc gtcctcacgg ggacgggcat tgccgcgctc
     1081 tggtccgcgt cctccgacta cgccctgtcc ctgggcaaat ccgccggcta cttcgccgcc
     1141 cgccaggcga tctacctcct gcccgccggc ctcgtcttcg cgttgtgcgc caccgtcgac
     1201 ctcgactcga tccgaaagcg caccgggctc atcaccctcg ccaccctcct cgtgctgctc
     1261 ctgcccttcg tgcccggcct cggggagaat cgcaacggtg ccacgaggtg gatcgacctc
     1321 ggcgtgacga ccttccagcc ctccgagctc tggaagctcg ccacggtgct gtacctcgcc
     1381 cacatcctgg acaagaaggc cgaccgtatc tcggagagcc ccggcgtcct catcccgccc
     1441 ttcctcctcg tcgcccttgg ctgcatcgtc gtctacctgc agaccgattt ctcgacggcc
     1501 gtcgtcatcg gcgtggccgc cgccgcggtc ttctgggcct cgggcgcgcc ggtctccttc
     1561 ttcctcgggc tgggggccgc cgcggcgccg ctggccgcgc tctcggtcct gacctcggac
     1621 ttccggctca ggcgcatcct gaccttcatg ttccccgcct acgagcccca tggccagggc
     1681 taccaggtcc tgggttccat ccgggccatc cgctcgggcg gcctggtcgg caaggggctc
     1741 ggcctgggca cgctcaagca gggctcgatc cccgaggtgc actcggactt cgtgttctca
     1801 gcctggaccg aggagaccgg cctcgtcggc gtcctcgtcg ttctcgcgct gtgggccttc
     1861 atcgcctggc ggggcttcaa ggtcgcgttg ggcgaaaccg accgctatag gtccagcctg
     1921 gtctgcgggc tgaccttcct cctctgcatc gaggtcctcg tcaacctcgg cgtcgtttcc
     1981 ggcgccgtcc ccgcgaccgg catggccctg cctttcttct cggccggcgg ctcgtccctc
     2041 ctgtccacgg cagtcgcctg cgggctcatc tacaacgcca gccgctctcc cgcgaacgcg
     2101 agagcgcgcg gcccgaacgc gctcgcggcg ggggaggtcg gcgatgtctg agctcgccgt
     2161 ccgcgcccag cacatcggct cgggcccggt ggggaatcgc gtcggcgcgg ccaagagccg
     2221 cgttggtgcg gccgagaacc gcgccggccg gcgccccttc gccgccaccg ccgcggtggg
     2281 cggccgaggc tacagcg
//
LOCUS       DASSQV010000015         4305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_626645, whole genome shotgun sequence.
ACCESSION   DASSQV010000015 DASSQV010000000
VERSION     DASSQV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626645"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..911
                     /locus_tag="VFL04_00255"
     CDS             81..911
                     /locus_tag="VFL04_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7838157.1"
                     /translation="MVQKDRLALATGYAFLCVAAIVDLVPFVWMLLSSLKPQAEIFSF
                     PPSLLPKQPSFRNYAAAWNAGGLNFARMFMNSMIVAIPATALCILSSSLAAFAFARIR
                     FRGRDTLFMCFVAAIMMPTMMVMIPTFLLMRPFIDTFVPLIARVMFGQAFVIFLFRQF
                     FMTIPKELDDAALVDGYSKFRIWWSIIMPLSKPVIATLVVFVFQQVYNDFLYPLIFIN
                     SKTNFTVQLGLASFRGIYVTRYDLLMAASVFTLVPILVLFLAAQRYFVEGVVMSGLKG
                     "
     gene            965..2890
                     /locus_tag="VFL04_00260"
     CDS             965..2890
                     /locus_tag="VFL04_00260"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HET7838158.1"
                     /translation="MVSVDARQVKITDPFWSRYRELVRKVMIPYQWKVMNDRVPGAEK
                     SHVIANFRIAAGLEDGGFYGFRFQDSDLAKWLEAVAHSLAWHPDPALEALADGAIELV
                     GRAQGADGYLNTYFTIAVPELRWKNERDGHELYVSGHFIEAAVAYYRATGKRALLDIM
                     MRNADHIDSVFGPEEGKKHGYPGHQVIEMALVQLHEATGEERYLRLAQYFIDERGKQP
                     NFFDLEAEARGEAGDRGKFGSRVYEYTQSHLPVREQRVATGHAVRAVYMYAAMADLAR
                     ISGDEGLASACRALWADLTRRQMFITGGIGQQEDWEGFSRDWDLPNDMTYNETCASVG
                     LVFWAKRMSELELKGDYYDVAERALYNGALAGMSLDGKKYFYANLLESVPEVMVGRRD
                     PDYAATTRRPWFACACCPPNLARLIASLSGYILSADDAGVAVQLYIGCEAELECGGAR
                     LGLRIEGSLPWSGGVRIALDAERPSRFALRLRIPGWCSGATARLGGAPLEGRLEDGYL
                     VIDREWRRGDLVELELPMPIQRIRANPLVRADAGRVALTRGPLVYCFEEADNGAGLWN
                     LTIPKDARLEAAEEPGLLGGVVTLSLEGRRRVGDWGDELYRSEEALTRGVRLRAVPYF
                     VWDNRGENEMSVWLREE"
     gene            2934..3677
                     /locus_tag="VFL04_00265"
     CDS             2934..3677
                     /locus_tag="VFL04_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HET7838159.1"
                     /translation="MVIVQPLSLEFEVRGFDCGYGGPLRLLSLANFFQETAGEHADQI
                     GYGVGKLLAEGRTWMLSRIDLRIDDLPRAGERVRVMTQPAGTERLFALRDLAMTGSDG
                     RSLARAVYAYLVVDLATRRLMRPDRLFGEAIPVGAAPHPLSGHLFDLPPAAATEVSFA
                     QRAWARHIDHNGHVNNAWLLDWLADAAPEDQRPVAPMAMRVEFLAEALEGDELLAAWG
                     RAEGGILSELRRGGEIIARAFLDHRPLLG"
     gene            3721..>4305
                     /locus_tag="VFL04_00270"
     CDS             3721..>4305
                     /locus_tag="VFL04_00270"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET7838160.1"
                     /translation="MSIERRNPVPSDIDIAQEAKLRTIVDVADGYGICNECIDHFGKY
                     KAKVRLEFLKDNMEKPVRAKYIDVTAITPTPLGEGKTTTTVGLTQGLGFLGRKAIAAI
                     RQPSMGPTFGIKGGAAGGGYSQIVPMADFNLHLTGDTHAVSVAHNLCSTALDARIYHE
                     SRWSDSYFEKIGLRKLNFDPYETWWNRVVDMNDRS"
BASE COUNT          726 a         1481 c         1388 g          710 t
ORIGIN      
        1 cctgatcctc tcggtgaccc tggtccagac caaggtcctc ggcaagcgcg tccaatacga
       61 cctctagaag gagctggcgg atggtgcaga aagacaggct tgccctcgcg acgggatacg
      121 cgttcctctg cgtcgctgcc atcgtcgacc tcgtcccatt cgtgtggatg ctcctgtcct
      181 cgctcaagcc ccaggccgag atcttcagct tcccgcccag cctcctgccc aagcagccct
      241 ccttccggaa ctacgcggcc gcctggaacg ccgggggact caatttcgcg cggatgttca
      301 tgaactccat gatcgtggcc atccccgcga ccgccctctg catcctctca tcctcgctcg
      361 ccgccttcgc cttcgcgagg atccgcttca ggggccggga taccctgttc atgtgcttcg
      421 tggcggccat catgatgccg accatgatgg tgatgatccc gaccttcctc ctcatgcgcc
      481 cattcatcga caccttcgtc cccctcatcg ccagggtcat gttcggccag gccttcgtga
      541 tcttcctgtt ccggcagttc ttcatgacca tcccgaagga gctcgacgac gccgcgctgg
      601 tcgacggcta ctccaagttc aggatctggt ggagcatcat catgccgctc tcgaagcccg
      661 tgatcgccac cctggtggtg tttgtcttcc agcaggtcta caacgacttc ctctatcccc
      721 tcatcttcat caactccaag acgaacttca ccgtacagct cggcctggcc tccttccggg
      781 ggatctacgt gacgcgctat gatctcctga tggcggcctc ggtcttcacc ctcgttccca
      841 tcctcgtcct cttcctcgcc gcccagcgct actttgtcga gggcgtcgtg atgtcggggc
      901 tcaaaggcta ggatcccgga acggaatccg catcggtccc cacaacagag caaggagcgc
      961 gagcatggtc agcgtcgacg caagacaggt gaagataacc gaccccttct ggtcgagata
     1021 ccgggaactc gtgaggaagg tcatgatccc ctaccagtgg aaggtcatga acgaccgtgt
     1081 ccccggggcc gagaagagcc acgtgatcgc gaacttcagg atcgctgccg ggctcgagga
     1141 tggcgggttc tacggcttcc gcttccagga ctcggacctg gccaaatggc tcgaggccgt
     1201 cgcgcacagc cttgcctggc atcccgatcc tgccctggag gccctggccg acggggccat
     1261 cgagctcgtg ggcagggccc agggcgccga cggctacctg aacacctact tcacgatcgc
     1321 cgtccccgag ctgcgctgga agaacgaaag ggacgggcac gagctctacg tctcgggcca
     1381 cttcatcgag gccgccgtcg cctactacag ggccaccggc aagcgcgccc tcctcgacat
     1441 catgatgagg aacgccgacc acatcgactc ggtcttcggg ccagaggagg gcaagaagca
     1501 cggctatccg gggcaccagg tgatcgagat ggccctggtc cagctgcacg aggccacggg
     1561 ggaggagcgc tatctgcgcc tcgcccagta cttcatcgac gagaggggca agcagcccaa
     1621 cttcttcgac ctcgaggccg aggccagggg cgaggcaggg gacaggggca agttcggctc
     1681 gcgggtctac gagtacaccc agtcccacct cccggtgcgc gagcagaggg tcgccacggg
     1741 gcacgcggtg cgggccgtct acatgtatgc cgcgatggcc gacctcgcca ggatctcggg
     1801 cgacgagggc ctggcctcgg cctgccgcgc cctctgggcc gacctcacga ggaggcagat
     1861 gttcataacg gggggcatcg ggcagcagga ggactgggag ggcttttcgc gcgactggga
     1921 cctgccgaac gacatgacct acaacgagac ctgcgcctcg gtcggcctcg tcttctgggc
     1981 caagcggatg agcgagctcg agctgaaggg ggactactac gacgtcgccg agcgcgccct
     2041 ctacaacggg gctttggcgg gcatgtccct tgacgggaag aagtatttct acgcgaacct
     2101 cctcgagtcc gtccccgagg tcatggtggg gaggagggac ccggactacg cggcgacgac
     2161 gcgaaggccc tggttcgcct gcgcctgctg tccgccgaac ctcgcccgcc tcatcgcctc
     2221 gctctcgggc tacatcctct cggccgacga cgcgggagtg gcggtccagc tctacatcgg
     2281 ctgcgaggcc gagctcgagt gcggcggagc gaggctcggc ctcaggatcg agggaagcct
     2341 tccctggtcg ggcggagtcc gcatcgctct tgacgccgag cgccccagcc gtttcgccct
     2401 caggctccgg atccccggct ggtgctcggg ggccacggcg cggctcggcg gggcgcccct
     2461 cgaggggcgg ctcgaggacg gctatttggt catcgaccgc gagtggagga ggggcgacct
     2521 cgtcgagctc gagctgccca tgcccatcca gcggatcagg gccaatcccc tggtgcgcgc
     2581 cgacgccggc agggtcgccc tcacccgggg ccccctggtc tattgcttcg aggaggccga
     2641 caacggagca gggctctgga acctgacgat ccccaaggac gcgaggctcg aggcggccga
     2701 ggagcccggc ctgctcggcg gggtcgtgac cctcagcctc gagggcaggc gccgagtggg
     2761 ggactggggc gacgagctct acaggagcga ggaggctctg acccggggcg tcaggctcag
     2821 ggccgtgccc tacttcgtct gggacaaccg gggcgagaac gagatgagcg tctggctgcg
     2881 cgaggagtag cggggggaag ccgcgtggcc ctgggcctgc tatagtcgcg ggcatggtga
     2941 tcgtacagcc actttcgctc gagttcgagg tcaggggttt cgactgcggc tacggcggcc
     3001 cccttcgcct cctctccttg gccaacttct tccaggagac ggccggcgag cacgccgacc
     3061 aaataggcta cggggtgggc aagctcctcg ccgagggcag gacctggatg ctctcgcgca
     3121 tcgacctcag gatcgacgat ctgccccgcg ccggcgagcg ggtgagggtg atgacccagc
     3181 ccgcgggcac cgagcggctc ttcgccctcc gcgacctcgc catgaccggg agcgacggga
     3241 ggagcctcgc cagggccgtc tacgcctacc tcgtcgtgga cctcgccacg cggcggctca
     3301 tgcgcccgga caggctcttc ggcgaggcga tccccgtcgg cgccgcgccc catcctctgt
     3361 ccggacacct cttcgatctg ccgcccgccg cggcgaccga agtctcgttc gcgcaacgcg
     3421 cctgggcccg ccacatcgac cacaacggcc acgtcaacaa cgcctggctc ctcgactggc
     3481 tcgccgacgc ggcgccggag gaccagcggc ccgttgcccc catggccatg cgggtcgagt
     3541 tcctggccga ggccctcgag ggggacgagc tcctcgcggc ctggggccgg gccgaggggg
     3601 ggatcctctc cgagctcagg cggggaggcg agatcatcgc ccgcgccttc cttgaccacc
     3661 gccccctcct gggttagcct ttgggccgaa ggcttcaatg ccgaccctag gaggcggatc
     3721 gtgtcaatcg agaggcgtaa cccggtaccg agcgacatcg acatcgcgca ggaagccaag
     3781 ctcaggacca tcgtcgacgt ggccgacggc tatggcatct gcaacgaatg catcgaccat
     3841 ttcgggaaat acaaggccaa ggtcaggctc gagttcctca aggacaacat ggaaaagccc
     3901 gtgagggcca agtacatcga cgtgacggcg ataacgccca cccccctggg cgagggcaag
     3961 acgacgacga cggtgggcct gacccagggc ctcggcttcc tggggcgcaa ggcgatagcc
     4021 gccatccgcc agccctcgat ggggcccacc ttcggcatca agggaggggc cgcgggcgga
     4081 ggctattcgc agatcgtgcc catggccgac ttcaacctcc acctcacggg cgacacccac
     4141 gcggtctcgg tcgcgcacaa tctctgctcg acggccctcg acgccaggat ctaccacgaa
     4201 tcccgctggt ctgactcgta tttcgagaag atcggcctgc gcaagctcaa cttcgacccc
     4261 tacgagacct ggtggaaccg ggtagtggac atgaacgacc gctcg
//
LOCUS       DASSQV010000016         5101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_129768, whole genome shotgun sequence.
ACCESSION   DASSQV010000016 DASSQV010000000
VERSION     DASSQV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129768"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFL04_00275"
     CDS             <1..265
                     /locus_tag="VFL04_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET7838161.1"
                     /translation="EEWDANKVYDRIGTEELTTEILGVRVPKLEIPVKPGRNIPIIIE
                     TAAMNERLKKMGYHSAQEFNQNVLRWLESENARALYFNRDDYL"
     gene            350..616
                     /locus_tag="VFL04_00280"
     CDS             350..616
                     /locus_tag="VFL04_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET7838162.1"
                     /translation="MNEATVTIKNRAGIHARPAALIVQTASKFASKIWLEKDSDRINA
                     KSIMGIITLGASFGTLIRIISDGADEEAATAAILGLFESKFEED"
     gene            630..2393
                     /locus_tag="VFL04_00285"
     CDS             630..2393
                     /locus_tag="VFL04_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7838163.1"
                     /translation="MAGPVAPRRRLFPDLLTITLIYVLVALGAYVLARQILGGAPDFP
                     GLERQLPLAVLAILPLSLAIILVLRVRTLVIELRVGRYGSRLRAKLAALFLLTVAAAS
                     LPQGAFLLRLATRAQAASSSAEIRKALAAGNRLALAWYDEELSRIERAAAELPVLDRK
                     GAGAVQLLDALRGREPRMEALEVFERARSLDFAGAPSARLAAPPPAPTRGTQGTLLPA
                     SASGDASRIRYAAAWSPGGSQGYTVLTLRLPESLGQTAELLGRASRTAELLAPFSARW
                     SLLLGLLYAFLVLPLLFLAGLLGLAAADLVSEPLASLEDATRRVAAGDFGVRLIVKPG
                     DETGHLVASFNRMLGEIERYRSDELRKEKIDAWKDIAQRLAHELKNPLTPIRLAAERV
                     LRRWKLDQGKAASIIESSMLAIVKEVEGMDALLSDFRSFASLPEPQRYWVELRPIVEE
                     SIALYAASYPEVRFSVDGVAEGLSLRVDRAAVKRALGNLISNAIDAMEGAGEIEFTAD
                     LVKAADSRYCRLRVRDNGRGIPASVGDKVFVPYFTTKPEGTGLGLAIVERIVADHGGE
                     IRFESEEGAGATFFVDLPLDR"
     gene            2410..3783
                     /locus_tag="VFL04_00290"
     CDS             2410..3783
                     /locus_tag="VFL04_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7838164.1"
                     /translation="MATILVIDDEPGIRSTVRDILEDEGHRVLLAEDAVIGLELMSVE
                     RPDLAILDVWLPRMGGLDALAEIKRRRPELEVVVVTGHGTIDMAVRALKLGAFDFIEK
                     PLSMDRLLTSARNALEIGELRRENKNLKRAASSLAGIEPLIGSSPAMAEIRALIDQAA
                     GSDARILITGENGTGKELVARSIHHGSRRAERPFVEVNCAAIPDTLLESELFGHERGA
                     FTDAVSRRAGRFETADGGTLFLDEVADLSLAGQAKVLRVLQEMRFERLGSEKTISVDV
                     RVIAATNKDIRSEIAAGRFREDLYFRLAVVPIHVPPLRERRGDVLDLCRYFLDKAASA
                     GAPVREISAEGLELLGAREWLGNVRELANFLERLAVMSDERLVSAATVARILGEGGQR
                     RQEPGIRERYGNLNLAEARELFERDYLVQKLRECAYNIPKAAEAAGISPSGLHAKVKK
                     YGIEQER"
     gene            3780..4379
                     /gene="lexA"
                     /locus_tag="VFL04_00295"
     CDS             3780..4379
                     /gene="lexA"
                     /locus_tag="VFL04_00295"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET7838165.1"
                     /translation="MKTLTVRQQEVLDFIASFLSEHAYPPTIREIADRFSISVKGAYD
                     HVKALERKGFLRLGENRSRALELLRREKPELMVVELPLLGVVAAGRPIYADENFSGTV
                     QVPADIVKASSCFALAVRGDSMKDAGIFHGDIAVIEQRQTADNGEIVVAMIDESVTLK
                     RFFRENNRVKLVAENPAYAPIYTQDAKIIGRLRGLIRKY"
     gene            4386..>5101
                     /gene="holA"
                     /locus_tag="VFL04_00300"
     CDS             4386..>5101
                     /gene="holA"
                     /locus_tag="VFL04_00300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET7838166.1"
                     /translation="MRIESVYLLAGPEVGKRRAFTDDLRAAVASADGQAAEEHRLYAS
                     ETGVAELLALLRNGSLFSTRRIVEYRGAELAKGKDELKELAAYIASPASDAILLLITE
                     AFYIEKALEEAVGKERKKTFYEMFENEKPRWIRSKLRELGFGIDDEGVEALLELVEND
                     SSALEAACARLAVVYQPGRELGEEEVEAAISHTRQEDAFSLFDRVVTGELDEALEVLE
                     AVLADRRGDAVQVISALIWG"
BASE COUNT          890 a         1779 c         1636 g          796 t
ORIGIN      
        1 cgaggagtgg gacgctaaca aggtctacga caggatcggc accgaggagc tgaccacgga
       61 gatcctgggc gtccgcgtcc cgaaactcga gatcccggtc aagcccgggc ggaacatccc
      121 gatcatcatc gagacggcgg cgatgaacga gaggctcaag aagatgggct accactccgc
      181 ccaggaattc aaccagaacg tcctgcgctg gctggagagc gagaacgcgc gggccctgta
      241 cttcaacagg gacgactacc tgtgagcgag aaagcacgcg gcgccgcgcc acaaggcatg
      301 gggccgcgga gatgcgggcg ctcgatcgcc cccgactgag gaaggcgcaa tgaacgaggc
      361 gacggtgacc atcaagaaca gggccggcat ccatgccagg cccgctgccc tcatcgtcca
      421 gacggcgagc aagttcgctt cgaagatctg gctggagaag gactccgacc gcatcaacgc
      481 caaatccatc atggggataa tcacactcgg cgcgagcttc ggcaccctca tcaggatcat
      541 ctccgacggg gcggacgagg aggcggccac ggccgccatc ttggggctct tcgagtccaa
      601 gttcgaagag gactaggggc cggcaggcga tggccggacc cgtcgcgcct cgacgtcgcc
      661 tcttccccga cctgctcacg atcaccctga tctatgtcct cgtcgccctc ggcgcctatg
      721 tgctggctcg tcagattctc ggcggcgcgc ccgacttccc cggcctcgaa aggcagcttc
      781 ccctcgcggt cctggccata ctccccctct ccctggccat catcctcgtg ctcagggtca
      841 ggacactcgt cattgagctg cgcgttggcc gatacggctc caggctgagg gccaagctcg
      901 ccgcgctttt cctcctcacg gtcgccgcgg cctccctccc tcagggcgcc ttcctcctca
      961 ggctcgcaac gagggcccag gcggcctcct cctcggccga gatcaggaag gccctcgccg
     1021 ccggcaacag gcttgccctg gcatggtatg acgaagagct ctcccgcatc gagcgcgccg
     1081 ccgccgagct gccggtcctg gacagaaagg gggccggggc cgtccaactc ctcgacgccc
     1141 tccgcggccg ggagccccgg atggaggccc tcgaggtatt cgagcgcgca aggagcctcg
     1201 acttcgccgg cgccccatcg gcccgcctcg ccgccccgcc cccagcgccc acgaggggga
     1261 cgcaggggac cctcctcccc gcctcggcct cgggggacgc gagcaggatt cggtacgccg
     1321 ccgcctggag ccccggtggg tcccagggtt acacggtcct caccctgagg ctgcccgaaa
     1381 gcctcggcca gaccgccgag ctcctcgggc gggcctcgag gacggccgag ctcctcgctc
     1441 ccttctcagc gcgctggtcg ctcctgctcg gcctcctgta cgccttcctt gtcctgcccc
     1501 tgctctttct ggccggcctc ctcggcctcg cggcggccga cctcgtgtcc gagcccctcg
     1561 caagcctcga ggacgcgacg cgtcgcgtcg ccgcgggcga cttcggcgtc cgccttatcg
     1621 tgaagcccgg cgacgagacc ggccatctcg tcgcctcctt caatcgcatg ctgggcgaga
     1681 tcgagcgcta ccggagcgat gagctcagga aggagaagat cgacgcgtgg aaggacatcg
     1741 cccagcgcct cgcccatgag ctcaagaatc cgcttacgcc gatccgcctc gccgcagaga
     1801 gggtcctccg gcgctggaag ctcgaccagg gcaaggccgc ctccatcatc gagagctcga
     1861 tgctcgccat cgtgaaggag gtcgagggca tggacgccct gctctcggat ttccgctcct
     1921 tcgcctccct ccccgagccc cagcgctact gggtggagct ccggcccatc gtcgaggagt
     1981 cgatagccct ttatgcggcc tcctaccccg aggtgcgctt ttcggtggac ggcgtcgccg
     2041 agggcctctc gctccgcgtg gaccgcgccg ccgtcaagcg cgccctcggc aacctgatct
     2101 ccaacgcgat agacgccatg gagggcgcgg gcgaaatcga gttcacggcc gaccttgtta
     2161 aggccgccga ttcacggtat tgtaggcttc gggtcaggga caacggccgg gggatcccgg
     2221 cttccgtggg cgacaaggtc ttcgttccct acttcacgac gaagccagag ggcacgggac
     2281 tcggcctcgc gatcgtcgaa cggatcgtcg ccgatcacgg cggcgagata cgcttcgaat
     2341 cggaggaggg cgctggcgcc accttcttcg tcgacctccc gctggatcgc tagggaagca
     2401 ggaaccgcga tggctacgat tctggtgatc gacgatgaac cgggcatcag atccacggtc
     2461 agggacatcc tcgaggacga gggccatagg gtcctcctcg ccgaggacgc ggtcatcggc
     2521 ctcgagctca tgtccgtgga gcgccccgac cttgcgatcc tcgacgtctg gctgccccgc
     2581 atgggtgggc tcgacgccct cgccgagatc aagcgaaggc gccccgagct cgaggtggtc
     2641 gtcgtcaccg gccacggcac gatcgacatg gctgtgaggg ccctgaagct gggcgccttc
     2701 gacttcatcg aaaagcctct ctcgatggac aggctcctga cctcggcgcg caacgcgctt
     2761 gagatcggcg agctcaggag ggagaacaag aacctcaagc gggccgcctc cagccttgcg
     2821 gggatcgagc ccctcatagg ctcctctccc gccatggcgg agatacgcgc cctcatcgac
     2881 caggccgccg gcagtgatgc gcgcatcctc atcaccggcg agaacggcac cggcaaggag
     2941 ctggtcgccc ggagcatcca ccatggcagc cggagggccg agcggccttt tgtcgaggtg
     3001 aactgcgccg ccataccgga caccctcctc gagagcgagc ttttcggcca cgagcgcggg
     3061 gccttcaccg acgcggtctc ccggagggcg ggacgtttcg agacggccga cggaggcacc
     3121 ctcttcctcg acgaggtcgc cgacctctcc ctcgccggcc aggccaaggt cctcagggtc
     3181 ctccaggaga tgcgcttcga gcgtctcggc tcggagaaga caatcagcgt cgacgtcaga
     3241 gtgatcgcgg ccaccaacaa ggacatccgc tccgagatag cggctggccg gttcagggag
     3301 gacctctatt tccgcctcgc cgtcgtgccc atccatgtcc cgcccctgag ggagcgccgg
     3361 ggcgacgtcc tcgatctctg ccgctacttc ctcgacaagg cagcgtcggc tggagcgccc
     3421 gtccgcgaga tctccgccga gggcctcgag ctgctgggcg ccagggaatg gctgggaaat
     3481 gtgagggagc tcgcgaattt cctcgagcgc ctggccgtca tgagcgatga gcgcctcgtc
     3541 tcggccgcga ccgtggcccg gatactcggg gaggggggcc agcgccgcca ggagcccggc
     3601 atcagggagc gctacggcaa cctcaacctc gccgaggcgc gcgagctttt cgagcgggac
     3661 taccttgtac aaaaactccg ggaatgcgcc tataatatcc ccaaggcggc cgaagccgcg
     3721 gggatcagcc cgagcgggct gcatgcaaaa gtcaagaaat acggtataga gcaggaacga
     3781 tgaagacact cacagttcgc cagcaggaag tgctcgactt catcgcctca ttcctctcgg
     3841 agcacgccta cccccccacg atccgggaga tcgccgaccg cttctccatt tcggtcaagg
     3901 gcgcctatga ccacgtgaag gcgctcgagc ggaagggctt cctgaggctg ggcgagaacc
     3961 gctcacgtgc cctcgagctg ctcaggaggg agaagcccga gctcatggtc gtcgagctgc
     4021 ccctcctcgg cgtcgtcgcc gctggccgcc ccatctacgc cgacgagaac ttctccggca
     4081 cggtccaggt ccccgcggac atcgtaaagg cgagctcctg cttcgccctc gccgtgcgcg
     4141 gcgacagcat gaaggacgcg ggcatcttcc acggggacat cgctgtcatc gaacagcgcc
     4201 agactgccga caacggcgag atcgtcgtgg cgatgatcga cgaatcggtg accctgaaac
     4261 gcttcttccg cgagaacaac agggtcaagc tcgtagccga gaatccggcc tacgcgccca
     4321 tctacaccca ggatgccaag atcatcgggc gtctgcgcgg cctcatccgc aagtactgag
     4381 ccggcatgag gatcgaaagc gtctacctcc tggcgggccc cgaggtcggc aagcgcaggg
     4441 cgttcaccga cgatctccgc gccgcagtgg cctcggccga cggtcaggcc gccgaggagc
     4501 accgactcta cgcctcggag acgggcgtgg ccgagctgct cgccctcctc aggaacgggt
     4561 cgctgttctc gacccgccgc atcgtcgagt accggggggc ggagctcgcc aaaggcaagg
     4621 atgagctcaa ggagctggcg gcctacatcg cctcccccgc ttccgacgcc atcctccttc
     4681 tcatcaccga ggccttctac atcgagaagg ccctggagga ggctgtcggc aaggagcgca
     4741 agaagacctt ctacgagatg ttcgagaacg agaagccgcg ctggatcagg tccaagctcc
     4801 gagagctcgg cttcggcata gacgacgagg gggtcgaggc cctcctcgag ctggtggaga
     4861 acgacagctc agcacttgaa gccgcctgcg cccggctcgc cgtcgtctat caacctggca
     4921 gggagctcgg cgaggaggag gtcgaggcgg ccatctccca cacgagacag gaggatgcct
     4981 tcagcctctt cgaccgcgtc gttacggggg agctcgacga ggcccttgag gtgctcgagg
     5041 ccgtcctcgc cgaccgtagg ggggacgccg tccaggtgat ctcggcgctg atctggggct
     5101 t
//
LOCUS       DASSQV010000017         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_627047, whole genome shotgun sequence.
ACCESSION   DASSQV010000017 DASSQV010000000
VERSION     DASSQV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627047"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFL04_00305"
     CDS             complement(<1..218)
                     /locus_tag="VFL04_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET7838167.1"
                     /translation="MAKMSLEQLRRLREEKRREVASREVDGKDIQIIVGMGTCGIAAG
                     AKQTFDAVVEAVNEFGLGERVVIRQTGC"
     gene            complement(244..789)
                     /locus_tag="VFL04_00310"
     CDS             complement(244..789)
                     /locus_tag="VFL04_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7838168.1"
                     /translation="MHYCVADYVMDIAQNAVEAGARNVGIEYRETETAIEVEVRDDGR
                     GMSEEARGRALDPFFTDGIKHPGRSVGLGLPFLVQGVEQAGGDWDLESGPGSGTRVSF
                     SFPKGNADAPPEGDLPGLFRALLCLDGDHELVVHRRGGRRVGAQGYELRRSELEEALG
                     GLGTAASLGLLREYIESQEEA"
     gene            complement(803..1279)
                     /locus_tag="VFL04_00315"
     CDS             complement(803..1279)
                     /locus_tag="VFL04_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET7838169.1"
                     /translation="MAAAQIEFSEELTAFIDEWKDKPGSLIMMLHRIQEEFGFIPRLA
                     AERLSRAVALPLAKIYGVITFYHFFKTTRPGKHRIAICLGTACYLRGAQELADEARTI
                     LNIKGEEVTDDGLFSIDEVRCLGCCGLAPVLMIGNEVYGKVAKEALPEIIAKYREQ"
     gene            1446..>3390
                     /locus_tag="VFL04_00320"
     CDS             1446..>3390
                     /locus_tag="VFL04_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7838170.1"
                     /translation="MNVKAGLRRGASLAPASAVLVLVLCLAPGAPASAQAVNGAISYA
                     PASPPILLLGEWERYDGYQTEETLGSTAQVPALAELQVRAGLRDIPHIPAGAYTYRIL
                     VTVRGSPAEREYAFALPGIGGYRAVIVNGRKLYDSAAGSPQPSLYYFRDATSRISILV
                     QCAPGGRLLERAALLPAKPVFGELSAVAGQGTYDFAGVFSLIGFFLVQAVFVLALFVF
                     WRKNLDFLSFSIFLAASAILSYFKYGAILHGLPGLRDVEGMEAGYVMAIDVQNLGLSL
                     FLVSLFHRLLHRMVAVAVLIPPALLACAAVALPAFQRDLYAISVFYMGSFLLCALVFI
                     AAQAFRGNHQARWIVPAFVPAAGAIVYRIALPDSLPGAFLIEPLGQVFLAFMAMLMLV
                     KKVAGSFESVETLSDYVSSVSRTMSSFIPKEFLEYLDKSDVTDLRLGDHIRKKMTIFF
                     SDIRAFTALSERLTVEENFAFINSYLSRVVPIIRENGGFVDKYIGDAIMALYSGNAGA
                     DQAIRSAIAMQAKIVEYNGHRAKMGYAPISMGVGIHTGDLMLGVVGVHDRMENTVISD
                     SVNLSSRLQAITKAFNISLAISEQSFKELEDPGSYKYRFIGKVKVKGKAAPVSVFEIF
                     DGIEPELFERKMKANTFFEQGMLS"
BASE COUNT          536 a         1193 c          993 g          668 t
ORIGIN      
        1 atgcatccgg tctggcggat gacgaccctc tccccgaggc cgaactcgtt gacggcctcg
       61 acgacggcgt cgaaggtctg cttcgccccc gccgcaatgc cgcaggtgcc catgccgacg
      121 atgatctgga tgtccttgcc gtccacctcg cggctggcga cttcccgcct cttctcctcc
      181 cggagcctgc gcagctgctc aaggctcatc ttcgccatat gttcgtctcc tttccgttcc
      241 ggttcatgcc tcttcctgcg attcgatgta ttcccgcaag aggcccaggg aggcggccgt
      301 cccaagaccc ccgagggcct cctcgagttc gctccgcctg agctcgtagc cctgggcccc
      361 aacccgccgc cccccgcgcc tgtggaccac cagctcgtgg tcgccgtcga ggcaaaggag
      421 ggcgcggaag aggccaggga ggtcgccctc gggaggggcg tccgcgttcc ccttcgggaa
      481 gctgaagctc acccgggtgc ccgagccggg cccgctctcg aggtcccagt cgccgccggc
      541 ctgctcaact ccctggacga ggaagggaag gccgagcccc acactcctcc ccgggtgttt
      601 gatcccgtcc gtgaagaagg gatcgagggc ccgtccgcgc gcctcctcgc tcatgccgcg
      661 gccgtcgtcg cgcacctcga cctcgatcgc ggtctccgtc tccctgtact cgatgcccac
      721 gttcctcgcg ccggcctcga cagcgttctg cgcgatgtcc atgacgtagt cggcgacgca
      781 gtaatgcacg ggggcctttg tcctactgct cgcggtactt ggcgatgatc tccggcaagg
      841 cctccttcgc gacctttccg tagacctcgt tgccgatcat gaggacgggg gcgaggccgc
      901 agcagccgag acagcgcacc tcgtctatgc tgaacaggcc gtcgtcggtg acctcttcgc
      961 ccttgatgtt gaggatggtc ctcgcctcat cggcgagctc ctgggcccct ctgaggtagc
     1021 aggcggtgcc gaggcagatc gcgatgcggt gcttccccgg cctcgtggtc ttgaagaagt
     1081 ggtagaaggt gatcaccccg tagatcttgg cgaggggaag cgccaccgca cgcgagagcc
     1141 gctccgcggc gagcctgggg atgaagccga actcctcctg gatgcggtgg agcatcatga
     1201 tcaggctccc gggcttgtct ttccattcgt cgatgaacgc ggtgagttcc tccgagaact
     1261 cgatctgggc cgctgccata gatactcctc tatagataag gatatatcga tatattcttc
     1321 gcggcattat atccagagga aaatgtcctg acaaggccag gtcccggatt ttgtcaagca
     1381 aaaaagccgc attcccttcg gaacccgagt gtcccgctcc ctcctcctca ggctatactg
     1441 ggccaatgaa tgtaaaagca ggtttgcgcc gaggggcctc cctcgctccg gcctcggcgg
     1501 tgctggtcct cgtcctctgc ctcgccccgg gagcacctgc atcggcccaa gccgtgaacg
     1561 gcgccatttc ctatgccccg gcatccccgc ccatcctcct cctcggcgag tgggagcgct
     1621 acgatggcta tcagaccgag gagaccctgg gatcgactgc ccaggtcccg gccctcgccg
     1681 agctccaggt cagggccggc ctccgagaca tcccccatat cccggccggg gcctacacct
     1741 accgaatcct cgtgacggtc aggggcagcc ccgctgaaag ggagtacgcc tttgccctgc
     1801 ccgggatcgg cggctaccgc gccgtcatcg tgaacggccg gaagctgtat gactcggcgg
     1861 ccggctcccc gcagccctcc ctctactatt tccgggacgc cacaagccgc atctccatcc
     1921 tggtgcagtg cgctcccggg ggaaggctcc tcgagcgggc ggcccttttg cccgccaagc
     1981 ccgtcttcgg agagctgtca gccgtggcag gacagggaac ctacgacttc gccggggtct
     2041 tctcgctcat cggtttcttc ctcgtccagg ccgtgttcgt tctggccctc ttcgtcttct
     2101 ggcggaagaa cctcgatttc ctctccttct ccatcttcct cgctgcatcg gccatcctga
     2161 gctacttcaa gtacggcgcc atcctccacg gcctccccgg gctccgggac gtcgagggga
     2221 tggaggcggg ctacgtcatg gccattgatg tccagaacct gggcctgagc ctcttcctcg
     2281 tgtcgctctt ccaccgcctg ctccaccgga tggtggccgt ggccgtcctg atacccccag
     2341 ccctccttgc ctgcgccgcc gtggccctcc cagccttcca gcgggacctc tatgcgatat
     2401 cggtgttcta catgggcagc ttcctcctct gcgccctcgt gttcatcgcg gcccaggcct
     2461 tccgcggcaa ccaccaggcc cgctggatcg tgcccgcttt tgtgccggcg gccggggcca
     2521 tcgtgtaccg gattgccctg cctgactccc tcccgggagc cttcctgatc gagccactgg
     2581 ggcaggtgtt cctcgccttc atggccatgc tcatgctcgt caagaaggtc gcgggcagct
     2641 tcgagtcggt ggagaccctc tcggactatg tgagctcggt ctcgcggacg atgagcagct
     2701 tcatccccaa ggagttcctc gagtacctcg acaagtcgga tgtcaccgac ctgcggctcg
     2761 gcgaccatat ccgcaagaag atgacgatct tcttctcgga catccgcgcc ttcacagccc
     2821 tctcggagcg cctcaccgtc gaggagaact tcgccttcat caactcctac ctgtctcggg
     2881 tcgtgccgat catccgcgag aacgggggct tcgtcgacaa gtacatcggc gacgccatca
     2941 tggccctcta ttccggcaat gcgggagccg accaggcgat ccgcagcgcg atagcgatgc
     3001 aggcgaagat tgtcgaatac aacggccaca gggccaagat gggctacgcc cccatctcca
     3061 tgggggtggg gatccacacc ggcgacctca tgctgggggt cgtgggggtc cacgaccgga
     3121 tggagaacac ggtcatctcc gattcggtga acctttcatc gaggctccag gccatcacca
     3181 aggccttcaa catcagcctc gccatctccg agcagtcctt caaggagctt gaggaccccg
     3241 gctcctacaa gtaccgcttc atcggcaagg tgaaggtcaa gggcaaggcg gccccggtct
     3301 cggtcttcga gatcttcgac ggcatcgagc ccgagctctt cgagcgcaag atgaaggcca
     3361 acaccttctt cgagcagggc atgctgtcct
//
LOCUS       DASSQV010000018         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2367448, whole genome shotgun sequence.
ACCESSION   DASSQV010000018 DASSQV010000000
VERSION     DASSQV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367448"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1359)
                     /locus_tag="VFL04_00325"
     CDS             complement(19..1359)
                     /locus_tag="VFL04_00325"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7838171.1"
                     /translation="MLDLAPLVNKTVPFKFNGAELRLDLSHALFSSYEVDAGTRLLLK
                     TVARDEVLARSRRILDEGCGTGVIGLSVAKAFPEAEVTMRDRDSLALAFSERNRLANR
                     LKGRTAWKDPDTGAERLAVAAPRILAGLLADGREGGPWDYILSNLPAKAGAPVLAAFF
                     RRARATLAPGGRVAVVIVHTLAEAAELWIAEAGLEVVARGRGANHRVFVAEAGTAAGG
                     GAAGQGDGAAKPEGGAAAQPAPGGAEGLELGGIDLSTYVRSEAGFKLADRSYRARGFW
                     GLPEFDTIGHGSSLAAEVGQRACAGGLVREALVVNPGIGHMALWIAKALGPSRISAAS
                     RDLLSLAATGANLGSLPASSRPAYRALDALGTGELPAHSLDLIVDFLDVIPEYDWIAP
                     AWETAARLLKRGGILIAASGPTELTRLERRRPSGWSLVADKRRRGFAATAWRLS"
     gene            complement(1444..2001)
                     /locus_tag="VFL04_00330"
     CDS             complement(1444..2001)
                     /locus_tag="VFL04_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET7838172.1"
                     /translation="MIRGADIGLGTWLLIQGQPHVVVERELLGPLGSRVRFRATALRG
                     GHPVRQTIGAAELVEAAEALTVECQYQYDDGESYMFLDRASLEQIALAVAENEARGPY
                     LREGETYALVLWEGEPVDVRIPPKMVFTVAKAESRTRSDTPAGATKSVTTDTGLVVRV
                     PLFIAQGERIVVDTATNEYVERAWA"
     gene            complement(2024..2530)
                     /locus_tag="VFL04_00335"
     CDS             complement(2024..2530)
                     /locus_tag="VFL04_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7838173.1"
                     /translation="MESSNANQYLTFTLDGEQYAVQVAKVREVLEHTKITKLPRTAEF
                     MKGIINLRGTGVPVIDLRLKFGLPETPLTKDTSIIVMEVVSLDGKIVVGALADAVHEV
                     VDIEDGSIEPAPRFGTRLAAEFIKGVGKRDEGFIIILDIDRIFNVDEIAVLAAGREAA
                     GAEASAQA"
     gene            complement(2653..>3105)
                     /locus_tag="VFL04_00340"
     CDS             complement(2653..>3105)
                     /locus_tag="VFL04_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HET7838174.1"
                     /translation="AACVLGLLLLPFSPRVGPLAWDWSALAPACSSCRRCVEACPTGA
                     LEGGFERARCLQHWSAIDAELPPAIEAAWGSRLYGCDSCLEACPRFRPEPRARTGLGV
                     LGPGLPAAWFAATGEAEIRAALKGSALGLGWMSMAAFRRNAELAAKVV"
BASE COUNT          433 a         1079 c         1139 g          454 t
ORIGIN      
        1 gaacgttttc ccgccgccct aggacagcct ccaggcggtg gcagcgaagc cccgcctccg
       61 cttgtccgcc acgaggctcc agcccgaggg ccgcctcctc tcgagcctgg tgagctcggt
      121 ggggcccgag gcggcgatga ggatgccccc ccgcttgagg agcctggcag ccgtctccca
      181 ggccggggcg atccagtcgt actcggggat cacgtcgagg aagtcgacga tgaggtcgag
      241 ggaatgggcc ggaagctccc cggtcccgag ggcgtccagg gcccgatagg ccggcctcga
      301 ggaggccggg agggagccaa ggttggcccc cgtggcggcg agggagagga ggtcgcggga
      361 ggcggcgctg atccgggagg ggccaagggc cttggcgatc cagagcgcca tgtggccgat
      421 ccccggattg accacgaggg cctcgcggac cagaccgccc gcgcaggccc tctggcccac
      481 ttcggcggcg aggctcgagc catggccgat cgtgtcgaac tcgggcaggc cccagaagcc
      541 gcgggcccgg taggagcggt cggcgagctt gaagcccgcc tcgctcctca cataggtgga
      601 caggtcgatg ccgccgagct caaggccctc ggccccgccg ggggccggct gggccgcggc
      661 cccaccctcg ggcttggccg ccccgtcccc ttgtccggcc gccccgcccc cggcggccgt
      721 gcccgcctcg gcgacgaaga cgcggtggtt ggctcccctg ccccgggcga ccacctcgag
      781 gccggcctcg gcgatccaga gctcggcggc ctcggccaag gtatgcacaa ttacgacggc
      841 cacccggccg cccggcgcga gcgtggcgcg ggcgcggcgg aagaaggcgg cgagcaccgg
      901 ggctccggcc ttggccggca ggttggacag gatgtagtcc cagggtccgc cctcgcgccc
      961 gtcggcgagg agcccggcaa ggatccgggg agcggcgacc gccagcctct cggcccccgt
     1021 gtcggggtcc ttccaggcag tcctcccctt gagccggttg gcgaggcggt tgcgctccga
     1081 gaaggccagg gccaggctgt cgcggtcgcg catcgtcacc tcggcctcgg ggaaggcctt
     1141 ggcgaccgag agcccgatca ccccggtccc gcagccctcg tccaggatcc gccgcgagcg
     1201 ggcgaggacc tcgtcacggg cgacggtctt gaggaggagg cgggtccccg cgtcgacctc
     1261 gtaggacgag aaaagggcgt gcgagaggtc gagcctgagc tcggcgccgt tgaacttgaa
     1321 ggggacggtc ttgttgacga ggggggcgag gtcgagcatg atccactcta ccttcccggc
     1381 tcggccgctt tcaatgccgt cctggacccg ggtcgaggcg gggacccggg tccaggcggg
     1441 ggcctaggcc caggcccgct ccacgtactc gttggtcgcg gtgtccacca cgatcctctc
     1501 gccctgggcg atgaagagtg ggacgcggac cacgaggccc gtgtcggtcg tgacgctttt
     1561 tgtggccccg gccggggtgt cgctcctcgt gcggctctcg gccttcgcca ccgtgaagac
     1621 catcttgggg gggatcctga cgtcgaccgg ctcgccctcc cacaacacga gggcgtaggt
     1681 ctcgccctcc ctgaggtagg gccccctcgc ctcgttctcc gccacggcga gggctatctg
     1741 ctcgaggctg gcccggtcga ggaacatgta gctctcgccg tcgtcgtact ggtactggca
     1801 ctccaccgtc agggcctcgg cggcctcgac gagctccgcg gcgccgatgg tctggcggac
     1861 gggatgcccg cccctgaggg ccgtggccct gaagcggacc cgggacccga gcgggccgag
     1921 gagctcccgc tcgacgacga catggggctg gccctggatg aggagccagg tgccgagccc
     1981 gatgtcagcg ccgcggatca agcccatcgc gggccccggg ggcctaggcc tgggccgagg
     2041 cctcggcgcc cgcggcctcg cggcccgccg cgaggacggc gatctcgtct acgttgaaga
     2101 tgcggtctat gtccaggatg atgatgaagc cctcgtcccg cttgccgact cccttgatga
     2161 actcggccgc gagcctggtg ccgaagcgcg gggctggctc gatggagccg tcctcgatgt
     2221 cgaccacctc gtggacagcg tcggcgaggg cgccgacgac gatcttgccg tcgaggctca
     2281 ccacctccat gacgatgatg ctcgtgtcct tggtcagggg agtctcgggc aggccaaact
     2341 tgagcctgag gtcgatgacg gggactcccg tgcccctgag gttgatgatg cccttcatga
     2401 actcggcggt ccgggggagc ttggtgatct tcgtgtgttc gaggacctcg cgcaccttgg
     2461 ccacctggac cgcgtactgc tccccgtcga gcgtgaatgt gaggtattga ttggcgttcg
     2521 agctttccat tgcttccccc ctgatcaaga tcgcattcgt agatggttga aatatacttc
     2581 cgtcggagcc gcctgcccgc gcgcttcgct ccgccgccag tgctgccatt ctagacgccg
     2641 gggcctggtt cgtcaaacca ccttcgcggc gagctcggcg ttccgcctga aggcggccat
     2701 gctcatccag cccaagccga gggcgcttcc cttgagggcg gccctgatct cggcctcccc
     2761 cgtggcggcg aaccaggcgg cggggaggcc ggggccgagg acccccagcc ctgtcctggc
     2821 gcggggctcg ggccggaagc ggggacaggc ctcgagacag gagtcgcagc cgtagagcct
     2881 cgagccccaa gccgcctcga tggccggcgg aagctcggcg tcgatggcgc tccagtgctg
     2941 gaggcagcgg gctcgctcaa agcccccctc gagggctcct gtggggcagg cctcgacgca
     3001 gcggcggcag gaggagcagg ccggggccag ggccgaccag tcccaggcca agggccccac
     3061 gcgcggcgag aaggggagga ggaggagccc gaggacgcag gccgc
//
LOCUS       DASSQV010000019         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1870318, whole genome shotgun sequence.
ACCESSION   DASSQV010000019 DASSQV010000000
VERSION     DASSQV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870318"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VFL04_00345"
     CDS             <1..873
                     /locus_tag="VFL04_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7838175.1"
                     /translation="PRTSMIKELVSVQSGKVALCGLLFLPEGPGPWPAVIVCHGAFEY
                     KENHLALCESLVRAGIGALALDMSGHGGSGGERWHVEMASWVRDLTAAADLLQARPDI
                     DPVRLGAFGFSSGGTAALEAALVEPRLKALATLSATVRDMAGAEALGFRLLVALGKAS
                     LGLRGRELRLSLAWALKTQHFAYDAGVNRAILADGRMAEAYASLPFPGSADCYFADTL
                     ARIGALRSPCLVLHGAEDRLDPVETGRLLYEKLSCEKRLVILARSGHFAHLESGRFEL
                     FAEVVGWMRAHLGT"
     gene            884..1507
                     /locus_tag="VFL04_00350"
     CDS             884..1507
                     /locus_tag="VFL04_00350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7838176.1"
                     /translation="MGTLRWMGRQMASPEGRGGRLMCAFLVMQHAPITRWTMELLDLR
                     PGERLLDIGCGNGVALGMIARRTGEGTVAGIDNASLAVEIATRRNRKEVGAGRVSVTL
                     GEATCLPFPDRSFDKVLALESFYFWGDHLAGLREARRVLKPGGRLSIVMELLKSEADP
                     GKNLWLARKMDCPIFSETELLALLREAGFGPARARWRKAWLCVQASR"
     gene            complement(1559..2248)
                     /locus_tag="VFL04_00355"
     CDS             complement(1559..2248)
                     /locus_tag="VFL04_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838177.1"
                     /translation="MKRLIVMALAVFVLAPALLAAESIPELKLAAEAAAKAAAAAPGD
                     YAANWTAAMELRKYGAELVLQETQGWKDLAKAAAKEGMKYGEIAQKLDPKGIAGWYWY
                     GLCVGTYSDCVSILTALGEGLKGKTQKAFETSYALDKNYENGGPMLSLGRFWQVLPGI
                     AGRDLKKAEQLFNEYMSAYGSSKDANKDAWYFRGALYKDTGRKAEAKADLEKAVAMGQ
                     KDAPKLLAQLK"
BASE COUNT          345 a          814 c          857 g          423 t
ORIGIN      
        1 ccgcgaacat cgatgataaa agagctcgtg tcagtgcaat cggggaaggt tgcgctctgc
       61 ggccttctct tcctgcccga ggggccgggc ccttggcccg ccgtcatagt ctgccacggg
      121 gccttcgagt acaaggagaa ccacctcgcg ttgtgcgaaa gcctggtccg cgccggcatc
      181 ggggccctag ccctggacat gagcggccac ggcgggagcg ggggcgagcg ctggcacgtg
      241 gagatggcca gctgggtcag ggacctgacg gccgccgccg atctcctcca ggcgcggccg
      301 gacatcgacc cggtcaggct gggggccttc ggtttctcct cgggggggac cgccgcgctc
      361 gaggcggccc tggtcgagcc caggctcaag gcactcgcca ccctttcggc gacggtcagg
      421 gacatggccg gcgccgaggc cctgggcttc cgcctcctcg tggccctggg gaaggcgagc
      481 cttggcctcc gggggaggga gctgcgcctc tccctcgcct gggctctgaa gacccagcac
      541 ttcgcctatg atgccggggt gaaccgggcg atcctcgccg acgggcggat ggccgaggct
      601 tacgcctccc tgccattccc gggttcggcc gactgttact tcgcggacac cctcgcccgg
      661 ataggcgcgc tgcgcagccc ctgccttgtg cttcacgggg ccgaggaccg cctcgaccca
      721 gtcgagacgg gaagactcct gtacgaaaag ctctcctgcg agaagcgcct cgtcatcctg
      781 gccaggtcgg gccatttcgc ccatctggag tcggggcgct tcgagctctt cgccgaggtg
      841 gtcggctgga tgcgggccca cctcggcaca taggaggcga agcatgggca cgctcagatg
      901 gatgggaaga cagatggcct cgcctgaggg caggggcggg aggctcatgt gcgccttcct
      961 ggtcatgcag cacgcgccga tcacccgctg gaccatggag ctcctggacc tccgcccggg
     1021 tgagaggctg ctcgacatcg gctgcgggaa cggggtcgcg ctcgggatga tcgcccggag
     1081 gaccggcgag ggtaccgtcg ctgggatcga caacgcttcc ctggccgtcg agatcgcgac
     1141 gaggcgcaac cggaaggagg tcggggcggg gcgggtctct gtcactctcg gcgaagcgac
     1201 ctgtctgccc ttccccgatc gcagtttcga caaggtcctg gccctcgagt ccttctactt
     1261 ctggggggat catctcgccg gcctcaggga ggcgaggcgg gtgctcaagc cgggcggccg
     1321 gctctcgatc gtgatggagc tgctcaagag cgaggccgat cccggcaaga acctctggct
     1381 ggcccggaag atggactgcc ccatcttctc cgagaccgag ctcctggccc tgctgagaga
     1441 ggccggtttt ggcccggccc gggcccggtg gaggaaggcc tggctctgcg tccaggccag
     1501 ccgctaggac cggcgcccca ctccggcggg aggccggccc acgcggcccg aagccgccct
     1561 acttcagctg ggcgaggagc tttggcgcgt ccttctggcc catggccacg gccttctcga
     1621 ggtctgcctt ggcctcggcc ttcctgcccg tgtccttgta gagggctccc cggaagtacc
     1681 aggcgtcctt gttcgcgtcc ttgctcgagc cataggcgct catgtactcg ttgaacagct
     1741 gctcggcctt cttgaggtcc ctgccggcga tcccggggag gacctgccag aagcggccga
     1801 gggagagcat gggccccccg ttctcgtagt tcttgtcgag ggcataggag gtctcgaagg
     1861 ccttctgggt cttgcccttg aggccctcgc ccagggccgt gaggatgctc acgcagtccg
     1921 agtaggtccc gacgcagagc ccgtaccagt accagccagc tatgcccttc ggatcgagct
     1981 tctgggcgat ctcgccgtac ttcatgccct ccttagccgc tgccttggcc aggtccttcc
     2041 atccctgggt ctcctggagg acgagctcgg ccccgtactt gcggagctcc atggccgccg
     2101 tccagttggc ggcgtagtcg ccgggcgccg ctgcggcggc cttggccgcg gcctcggcgg
     2161 ccagcttgag ctcgggaatg ctctccgctg ccagcagggc gggcgcgagg acgaagacgg
     2221 cgagggccat aacgatgagt ctcttcatgg tttctccttg ggcggcgatc gtattcgagg
     2281 aagtctagtt tccccaaagg gcccggtcaa ggaacgccgg gcccggctcg gggccgcgat
     2341 gggacagcgc gctcggccca gccaggtggc ggagctccag ggaatttttg actgcggccc
     2401 cggcggcttc gcatattcgg aggagggaca gagctcggg
//
LOCUS       DASSQV010000020         6853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_752584, whole genome shotgun sequence.
ACCESSION   DASSQV010000020 DASSQV010000000
VERSION     DASSQV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752584"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFL04_00360"
     CDS             <1..258
                     /locus_tag="VFL04_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET7838178.1"
                     /translation="AIAEGRGRSALAASLRARHLECETPCPEMLDALRVPCIAERALV
                     YLYAEQNLLSAEQLRGAVRQLGLDREYLGRRLSDNRRPLEL"
     gene            294..1643
                     /locus_tag="VFL04_00365"
     CDS             294..1643
                     /locus_tag="VFL04_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7838179.1"
                     /translation="MPNKREPLPRTITVLFFARLINAAGNFVFPFITMLLTVKLGWSA
                     ARTGSFMSVMFLVSGVGMLAGGKFGDLWGRKRVIVACQLGAAACFLGCVALGLSPAMP
                     FVVAAANFLLNATWPVFNATVADLTPADQRKRAYALLYWGNNIGFSIGPLAAGFLFSS
                     HPGLMFAGNATALIIVCTLFLMWVPETLPDAAKAEEARHSAEAAEEGGLVRALLRRPI
                     LLGFALSMAVMNFVYAQHMFSLPVFLGSRLGDAGAKVFGAAMTVNGLTVVAATGLVTW
                     LLGRLKSLACMAVASSLYALGFGMLVLVAAPGLGISGPIVVYASTVVWTLGEIASATN
                     ANVFIASRSPLSHRSRMNSLVTWIGNSGSMLCPVLSGAWISGRGAQAIWPICAALGVI
                     GAVLMLLLHSADRSAGPADRGGVQDQADRGGAQDQVDRGGAQDQADRGRTPAAEVAR"
     gene            1687..3126
                     /locus_tag="VFL04_00370"
     CDS             1687..3126
                     /locus_tag="VFL04_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET7838180.1"
                     /translation="MSQDLRRMLEACAPPKAEWAGLRRVRSTGRGWEAKDGKFDSAYD
                     FTGEGYMVEVLYAGQFGYAATASRDPGALRAAAERALALARAASAWPVFRFDKAVRPA
                     TALAWESPRRLRSSLGSDVLSRHPIALTEAMRISDKIVQASAGLELRDIVTELVSTSG
                     AAVEQSTHILGWSFQAVAREGSVTQRRSANGHRGRTNQGGLELLDLEAGKADARRAAE
                     EAIELLSAQECPSMKADLVLAPDQMMLQIHESVGHPIELDRILGDERNFAGSSFVRLE
                     DIGSLRYGSELMNVSFDPTVPTEAASYGADDIGNAARKEYLIKDGILVRALGSLESQA
                     RSGLPGVANQRAQDWYRAPIDRMANLNLEPGTSALPDIIAGIERGVWMEANRSWSIDD
                     RRNKFQFGCEYARLIEEGRLTKTVRNPNYRGISASFWRNLFMVGDEASRGVFGTPNCG
                     KGEPSQVITVGHASPACAFRDVEIFGGGA"
     gene            3123..4475
                     /locus_tag="VFL04_00375"
     CDS             3123..4475
                     /locus_tag="VFL04_00375"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET7838181.1"
                     /translation="MSSASDLEARFDLSCELLFGELARGESLSMEFSGESSTFMRFNR
                     GVRQIGQVDRATVSFKYYRDGRTLVSGFETTGDRALDAEAAAAALSSARREAALLPED
                     PYPTIPSAEGRSRESFPGRLPEAERLPELLLGPASALQRAGADFVGIHAQGPVCRGAA
                     NDRGARHWFSTETFALDYSACLANGKAVKSLYAGREWDGAEYERRLGAELPKLEALSR
                     PEKTIVPGEYRVYLASDALNEFMPFFSWNGLSERQIREGESAFIALKEGRRLMSPRFS
                     LSQDFSLGIEPRFNELGDLAPESLPLIAGGKLANSLVSARSAKQYGVPSNASPEWEGL
                     RSAVIEAGELDEESAFEAIGTGLYVSNLHYLNWSDFDSARVTGMTRFACFWIEGGRIA
                     APIRDMRFDESLYRLFGDKLAALSSRRSLVSDTSSYDRRALGGSLLPGVLVEGFTFTL
                     "
     gene            complement(4568..5221)
                     /locus_tag="VFL04_00380"
     CDS             complement(4568..5221)
                     /locus_tag="VFL04_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7838182.1"
                     /translation="MLFEKDDPARIFELSAGIHLRSRRAAERALRPHGLTYAQYGGLL
                     ALAKGGSMSQSALAVALETDTTTAMVVRGSLERKGLVDRESHPDDGRVKMLKLTSKGH
                     KLAAAAMKDIDAFYAKLARLVSDADLKKTLPILERLNELATVAADGHNGAAKPAKPAA
                     QKASEPKARAKAAKLQKVASAKRAPASGNPAAGTAKAPARRLASAKRAAAPKAPKKR"
     gene            complement(5525..6709)
                     /locus_tag="VFL04_00385"
     CDS             complement(5525..6709)
                     /locus_tag="VFL04_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HET7838183.1"
                     /translation="MRETQRLFYEEPGLSGARARIIELGEGGRKLVLDRTVFYPEGGG
                     QPCDLGTIGGIALVSVAEEAGRIVHVLSEPIAAAPGAEVELALDAARRLDHSVQHSAQ
                     HLISAVFTRLCGAATLSFHLGASRSTIDLDMPPPSDDELAEVELRLEEAVAEDYPYLV
                     HLCPPERASDFPLRKAPPEGEEELRIVEIDGLDYSPCCGTHVSSSARLRMVKLTGVEK
                     YKGITRLSFVAGPRAFADYCALSRAARDSARALGCSLLELPERVARAAERLKALEASL
                     GRLERERAGLEADLAAAKDGGLPPFVSALYADRDADAAQEAVRALAARRAIALAASLP
                     ELTAVASAPSGAARLGERLRPMAEASGGRGGGGPSSFRAAFRDEPALRSFMRDASAEL
                     GG"
BASE COUNT          998 a         2492 c         2336 g         1027 t
ORIGIN      
        1 gccatcgccg agggcagggg caggtcggcc ctcgccgcga gcctgcgggc ccgccacctc
       61 gagtgcgaga cgccctgccc cgagatgctc gacgccctcc gcgtgccctg catcgccgag
      121 cgcgccctcg tctacctcta cgccgagcag aacctgctct cggccgagca gctgcgcggc
      181 gcggtgaggc agctcggcct cgaccgcgag tacctgggca ggaggctctc ggacaaccgc
      241 aggcccctcg agctgtagcc gcgcgggggc cgcctcgcta caatcggccg accatgccca
      301 acaagcgaga gcccctgccc cggaccatca cggtcctctt cttcgcccgc ctcatcaacg
      361 cagcgggcaa cttcgtcttc cccttcatca ccatgctcct caccgtcaag ctcggctgga
      421 gcgcggcgcg gacgggatcc ttcatgagcg tgatgttcct cgtgagcggt gtgggcatgc
      481 tcgccggggg caagttcggc gacctatggg gccggaagcg cgtcatcgtg gcctgccagc
      541 tgggggccgc ggcctgcttc ctcggctgcg tggccctggg cctttccccc gccatgccat
      601 tcgtcgtcgc cgccgccaac ttcctgctca acgcgacctg gcccgtcttc aacgcgacgg
      661 tggccgacct caccccagcc gaccagcgca agcgagccta tgccctcctg tactggggca
      721 acaacatcgg cttctccatc ggccccctcg ccgccggctt cctcttctcg agccaccccg
      781 gcctcatgtt cgcgggcaac gccacggccc tcatcatcgt ctgcaccctt ttcctcatgt
      841 gggtgcccga gaccctgccc gacgcggcta aggccgagga ggcccgccac agcgccgagg
      901 ccgccgagga gggaggcctc gtgcgggccc tgctccgccg gcccatcctc ctcggcttcg
      961 ccctcagcat ggccgtcatg aacttcgtct acgcccagca catgttcagc ctccccgtct
     1021 tcctgggctc gaggctcggc gacgcggggg ccaaggtctt cggggcggcg atgacggtga
     1081 acggcctcac cgtggtcgcg gcgacgggcc tcgtcacctg gctcctcggc cgcctcaaaa
     1141 gcctggcctg catggcggtg gcctcgagcc tctacgccct cggcttcggc atgctcgtcc
     1201 tcgtggctgc gcccggccta ggcatcagcg gtcccatcgt ggtctacgcg agcaccgtgg
     1261 tctggaccct cggcgagata gcctcggcca cgaacgccaa cgtgttcatc gcctcgcgct
     1321 cccccctctc ccaccggagc aggatgaact ccctcgtcac ctggatcggc aactcgggct
     1381 cgatgctctg ccccgtcctc tcgggggcat ggatctcggg gcgcggggcc caggcgatct
     1441 ggccgatctg cgccgccctc ggcgtcatcg gcgccgtcct catgctcctc ctccactcgg
     1501 ccgaccggtc agccgggccc gccgaccggg gcggcgtcca ggatcaggcc gaccggggcg
     1561 gcgcccagga tcaggtcgac cggggcggcg cccaggatca ggccgaccgg ggccggactc
     1621 cggccgcgga agttgcgcga taggcgaatc tgtagcatca ttgcccggat aaggagggcg
     1681 ccgctcatgt cacaggatct cagacgaatg ctcgaggcct gcgccccgcc taaggccgaa
     1741 tgggccggcc tgcgccgcgt gcgcagcacg ggacggggct gggaggccaa ggacggcaag
     1801 ttcgactcgg cctacgattt caccggcgag ggctacatgg tcgaggtcct ctacgccggc
     1861 cagttcggct acgccgccac ggcttcccgc gatccggggg ccctgcgggc ggccgcagag
     1921 cgggccctcg ccctggcccg ggcggcctcg gcctggcccg tcttccgttt cgacaaggcc
     1981 gtgaggccgg ccacggccct cgcctgggag agcccgcgca ggctccgctc gagcctcggc
     2041 tccgatgtcc tctcccgcca ccccatcgcc ctcaccgagg ccatgcgcat ctcggacaag
     2101 atagtccagg cctcggccgg gctcgagctg cgcgacatcg tcaccgagct cgtatccact
     2161 tcgggcgcgg ccgtcgagca gagcacccac atcctcggct ggagcttcca ggccgtcgcc
     2221 cgagagggca gcgtcacgca gcggaggagc gccaacggcc accgcggcag gaccaaccag
     2281 ggcggcctcg agcttctcga cctcgaggcg ggcaaggccg acgcgcggcg ggcggccgag
     2341 gaggccatcg agctgctctc ggcccaggaa tgcccctcga tgaaggccga cctcgtcctc
     2401 gcccccgacc agatgatgct ccagatccac gagtcggtcg gccaccccat cgagctcgac
     2461 aggatcctcg gcgacgagcg caacttcgcc ggctcctcct tcgtcaggct cgaggacatc
     2521 gggagcctgc gatacggctc cgagctaatg aacgtgagct tcgaccccac cgtccccacc
     2581 gaggccgcat cctacggggc cgacgacatc ggcaacgcgg ccaggaagga gtacctcatc
     2641 aaggacggga tcctcgtgcg cgccctcggc agcctcgaga gccaggcccg ctcgggcctc
     2701 cccggggtcg cgaaccagag ggcccaggac tggtaccggg ccccgatcga ccgcatggcc
     2761 aacctcaacc tcgagccggg aacatcggcg ctcccggaca tcatagcggg catcgagcgc
     2821 ggggtctgga tggaggcgaa ccgctcctgg tcgatcgacg accgccgcaa caagttccag
     2881 ttcggctgcg agtacgcccg cctcatcgag gagggcaggc tcacgaagac ggtcaggaac
     2941 ccgaactacc gcgggatctc ggcgagcttc tggaggaacc tcttcatggt aggggacgag
     3001 gccagccgcg gcgtcttcgg gacccccaac tgcggaaagg gcgagccgag ccaggtcatc
     3061 accgtgggcc acgcgagccc ggcctgcgcc ttccgcgacg tcgagatctt cggaggtgga
     3121 gcatgagctc ggcatcagac ctcgaggcca ggttcgacct gtcctgcgaa ctcctcttcg
     3181 gcgagctcgc ccggggcgag tcgctctcga tggagttctc cggcgagtcc tcgaccttca
     3241 tgcgcttcaa ccgcggcgtg aggcagatcg gccaggtgga cagggccacg gtgagcttca
     3301 agtactaccg cgacggcagg accctggtct cgggcttcga gaccacgggc gatcgcgctc
     3361 tcgacgccga ggccgcggcg gcggccctca gctcggcccg gcgcgaggcc gccctccttc
     3421 ccgaggaccc ctacccgacc atcccctcgg ccgagggccg gtcccgcgag agcttcccgg
     3481 gccggctccc cgaggccgag cgcctccccg agctcctcct cggcccggcc tcggccctcc
     3541 agagggcggg ggccgatttc gtcggcatcc acgcccaggg ccccgtgtgc aggggagcgg
     3601 ccaacgaccg gggggcccgg cactggttct ccaccgagac cttcgccctc gactactcgg
     3661 cctgtctcgc gaacggcaag gcggtgaagt ccctctacgc cggccgcgaa tgggacgggg
     3721 ccgagtacga gcggaggctc ggagccgagc tgcccaagct cgaggccctg agccggcccg
     3781 agaagaccat cgtccccggc gagtaccgcg tctatctggc gagcgacgcc ctcaacgagt
     3841 tcatgccctt tttctcgtgg aacggcctct cagagcgcca gatccgcgag ggcgagagcg
     3901 ccttcatcgc cctcaaggag ggccgcaggc tcatgtcccc gcgtttcagc ctctcgcagg
     3961 acttcagcct cggcatcgag ccccggttca acgagctcgg cgacctggcc cccgagagcc
     4021 tgcccctcat cgcgggcggg aagctggcca acagcctcgt ctcggcccgg agcgcgaaac
     4081 agtacggggt gccgtccaac gcctcgccgg aatgggaggg cctccgctcg gctgtgatcg
     4141 aggcgggcga gctcgacgag gagtcggcct tcgaggctat cggcaccggt ctctatgtct
     4201 cgaacctcca ctacctcaac tggagcgact tcgactcggc gagggtcacg ggcatgaccc
     4261 gtttcgcctg tttctggatc gagggcggca ggatcgccgc cccgatcagg gacatgcgct
     4321 tcgacgagag cctctaccgc ctcttcggcg acaagctcgc cgccctgagc tcgaggcgct
     4381 ccctcgtgag cgacacatcc agctacgaca ggagggccct cggcggctcg ctgctgcccg
     4441 gggtccttgt ggagggcttc accttcacgc tctagggcgg gccgcggccc cactatgccg
     4501 tcattcaaga aaaaggaccg gcctggtggc cggtcctttc gtttcggggc gcgtgccgcc
     4561 agagggccta gcgcttcttc ggcgccttgg gggccgccgc ccgcttcgcc gaggcgagac
     4621 gccgggccgg ggccttggcc gtgccggccg caggattgcc cgaggcgggg gcgcgctttg
     4681 cactcgcgac cttctggagc ttggcggcct tcgctcgggc cttgggctcg gaggccttct
     4741 gggccgcggg cttcgcgggc ttggcggcgc cattatggcc gtcggcggcc acggtggcca
     4801 gctcgttgag cctctcgagg atgggcaggg tcttcttcag gtcggcgtcg gagaccaggc
     4861 gcgcgagctt cgcgtagaag gcgtcgatgt ccttcatcgc ggccgccgcg agcttgtgcc
     4921 ccttcgaggt gagcttgagc atcttgacgc ggccgtcgtc ggggtgggac tcgcggtcga
     4981 cgaggccctt gcgctcgagc gagccgcgca ccaccatcgc cgtggtcgtg tcggtctcga
     5041 gggcgacggc gagggccgac tggctcatgg agccgccctt ggcaagggcg agaaggccgc
     5101 cgtactgggc ataggtgagg ccgtggggcc tgagcgcgcg ctccgcggcg cggcgagagc
     5161 ggaggtggat gcctgccgac agctcgaaga tcctcgccgg gtcgtctttt tcgaatagca
     5221 tttattcctc ctgtatcctg gacacgatca tagatagtat aatcatatct gtcaatacag
     5281 gtttcaaatg attcacttgg ggcagctttg cacctaaccc ctgacatcgc cctttcacgg
     5341 gctgaaacgg cgcgagcctg gcggcggctg ctataaattc cgaacgcctt tcggtgcgcg
     5401 ccccgaaggc tgaagcgagg cgccccgaag cttaggcgcg gcgccccgaa ggttcagcgc
     5461 gacgcaccga gggttcagcg cggcgcaccg aaggttatgc gcggggtcaa gctaggcgcg
     5521 gcgcctagcc cccgagctcg gccgaggcat cgcgcatgaa ggagcgcagg gccggctcgt
     5581 cgcggaaggc cgccctgaag gagctggggc ctccgccgcc ccggccgccc gaggcctcgg
     5641 ccatgggcct gaggcgctcg ccgagccggg cagcgccaga gggcgccgag gccacagcgg
     5701 tgagctcggg cagggaagcg gccaacgcga tggctctgcg ggctgcgagg gcgcggacgg
     5761 cctcctgggc cgcgtcggcg tcgcggtcgg cgtagagcgc cgagacgaag ggcgggagcc
     5821 ctccatcctt ggcggccgcc agatcggcct cgaggcccgc cctctcgcgc tcgagccgcc
     5881 cgagcgaggc ttcgagcgcc ttcagccgct cggccgccct cgcgacccgc tcgggcagct
     5941 cgaggaggga gcagcccagg gccctggctg agtccctcgc ggcccgcgag agggcgcagt
     6001 agtcggcgaa ggcccgcggt ccggccacga aggagagccg cgtgatgccc ttgtacttct
     6061 cgacgccggt gagcttcacc atgcggaggc gggccgagga ggacacatgg gtcccgcagc
     6121 agggactgta gtccaggccg tcgatctcca cgatcctgag ctcctcctcg ccctcgggcg
     6181 gggccttgcg cagggggaag tccgaggccc gctcgggcgg gcagaggtgg accaggtagg
     6241 ggtaatcctc ggccacggcc tcctcgaggc ggagctcgac ctcggccagc tcatcgtccg
     6301 aggggggagg catgtcgagg tcgatcgtgg agcggctcgc gccaagatga aagctcaggg
     6361 tcgcggcccc gcagagcctc gtgaagacgg cggagatgag gtgctgggcc gagtgctgga
     6421 ccgaatggtc gaggcgccgc gccgcgtcca gggccagctc cacctcggcc cccggcgccg
     6481 cggcgatggg ctccgagagg acgtggacga tcctccccgc ctcctcggcc accgagacaa
     6541 gggctatgcc gcctatcgtg ccgaggtcac agggctgtcc cccgccctcg gggtagaaga
     6601 ccgtccggtc gaggacgagc ttcctgccgc cctccccgag ctcgatgatc ctagccctgg
     6661 ccccggagag gccgggctcc tcgtaaaaga gtctctgggt ttccctcatg gcaggacgat
     6721 ggtacagcgc cgccgcccgg accgggaatc ggcccgcggc ggccggcacc tttgcgcgga
     6781 ggcccccggg ccggcccctt aactcgccgg cccccgggcc ggcaccttaa ctcgccggcc
     6841 cgggcgaggc tag
//
LOCUS       DASSQV010000021         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1124997, whole genome shotgun sequence.
ACCESSION   DASSQV010000021 DASSQV010000000
VERSION     DASSQV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124997"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..874
                     /locus_tag="VFL04_00390"
     CDS             383..874
                     /locus_tag="VFL04_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838184.1"
                     /translation="MAKPKDVIHLVVTQLDECFAGPAWHGASLYPTLAELDLAQAWYE
                     SAEGLSAWKVALHCAYWKWRVRKVLLAASGQSISRFGRSPADWPRLPKTLDAANWKAD
                     LGFLEDHHAALRAAVVEIPASLLWESYDSKGRIYHRLIAGAASHDAYHTAHVRNIGIP
                     GLI"
     gene            complement(911..1735)
                     /locus_tag="VFL04_00395"
     CDS             complement(911..1735)
                     /locus_tag="VFL04_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HET7838185.1"
                     /translation="MGQTLLITFRESLEAALVIGIVLGIIKQGGRPGLRSSVWVGLVA
                     GLVCSVVAAYAFIRALGQFEGREEQIFEGSVMAFGSILLATLILWTGSHAKGSELSGK
                     AGRALNGGWLAIATLVFTSVLREGVETVIYLGDSLMRKEASLVLAGLIGLGLAVAAGF
                     LFFAGGRRMSSGLFLQATSTLLLLFGAGMFARALGEFGEAGLVPPLVEPLWSIAPARE
                     GLPVPLMNDEGGLGSFLHGLFGYISSPSLTEVLGYLAYAAAILLVLWGRGRRRYRA"
     gene            complement(1753..2091)
                     /locus_tag="VFL04_00400"
     CDS             complement(1753..2091)
                     /locus_tag="VFL04_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET7838186.1"
                     /translation="MTFLYLTSDCLGSGTEEELGRKLLNLFLEKLAASDQRVDLIGCV
                     NGAVRLTTGEGPALESLKRLQARGSRIATCGTCLDHLGLRASLRIGEVGSMDATVQVL
                     ASADRVIKPC"
BASE COUNT          420 a          763 c          737 g          324 t
ORIGIN      
        1 tgcggcactc acgagcacaa gcgccaagaa gggaaatagc ttctccatca tatgtttgcg
       61 ctcatgggcc gagctcccga tgcacatttc aacccgacag ccactgaaag acgccatggt
      121 gagtatcttt caagatttct ggcaagacaa gagtgagctt ggaagcaaat ctcgggttat
      181 tttcccggga agtcagcaag gcatacccaa gacgaggcca agaagccgcc aaggtcgagg
      241 aactgtagtc gagagaaacc cacctcggtt cgagaccgcc ctaggccacg ctggccgccc
      301 gagccgccca gcggcccctg ccgttgacca cccaagatcg gccccagtat gcttctgcca
      361 gccccagcaa gaggagggcg cgatggcgaa accgaaggat gtcatccatc tcgtggtcac
      421 ccagctggac gagtgcttcg ccggcccggc ctggcacggc gccagcctct accctaccct
      481 cgcggagctc gacctcgctc aggcctggta cgagtccgcc gagggcctgt cggcgtggaa
      541 ggtcgccctc cactgcgcgt actggaagtg gcgcgtgcgc aaggtgctcc tggcggcgag
      601 cggtcagagc atcagccgtt tcgggcgctc gcccgccgat tggcccaggc tccccaagac
      661 cctggacgcg gcgaactgga aggccgatct cggcttcctc gaggaccatc acgcggcgct
      721 gcgcgcggcc gtcgtggaga tcccggcctc actcctctgg gagagctacg acagcaaggg
      781 ccgcatctat caccggctca tcgcgggggc cgcctcgcac gatgcctacc acacggccca
      841 tgtccgcaac atcggcatac cggggctgat ctagccgaac cgctcctctc gcccgaagta
      901 gatcacccgc ctatgcccga taccgcctgc ggcccctccc ccagagcacc aggaggatcg
      961 ccgcggcgta ggcgaggtaa ccgaggacct cggtgagcga ggggctcgag atgtagccga
     1021 agagcccgtg gaggaaggag ccgaggcccc cctcgtcgtt catcaggggg acgggaaggc
     1081 cctcccgcgc gggggcgatg ctccacaggg gctcaaccag gggagggacg aggccggcct
     1141 cgccgaactc acccagggcc cgcgcgaaca tgccggcccc gaagaggagg aggagggtcg
     1201 aggtcgcctg gaggaagagc cccgagctca tccgccttcc gcccgcgaag aagaggaaac
     1261 ccgcggcgac ggcgaggccg aggccgatga ggcccgcaag gacgagggag gcctccttcc
     1321 gcatgaggct gtcgccgagg tagatcaccg tctcgacccc ctcgcgcagg accgaggtga
     1381 acaccagggt ggcgatggcg agccagcccc cgttcagggc gcggccggcc ttgccgctga
     1441 gctccgagcc cttcgcgtgg ctgcccgtcc agaggatgag ggtggcgagg aggatcgagc
     1501 caaaggccat caccgagccc tcgaagatct gctcctccct cccctcgaac tgccccaggg
     1561 ccctgatgaa ggcgtaggcc gccaccacgc tgcagaccag gcccgcgacc aggccgaccc
     1621 agaccgagga gcggaggccc ggccgccctc cctgcttaat gatcccgagg acgatcccga
     1681 tgacgagggc cgcctcgagc gattccctga aggttatgag caacgtctgt cccatcagcg
     1741 actccctgag cctcagcagg gcttgatgac gcggtcggcg ctggcgagga cctggacggt
     1801 cgcgtccatc gacccgacct cgccgatccg cagcgaggcc ctgaggccga ggtggtcgag
     1861 gcaggtcccg caggtggcga tgcgcgatcc tcgggcctgg agcctcttga ggctctcgag
     1921 cgctggcccc tcgcccgtcg tgagcctgac cgcgccgttc acgcagccga tgaggtcgac
     1981 cctctggtcc gaggccgcga gcttttcgag gaagaggttg aggagcttcc tgccgagctc
     2041 ctcttcggtg ccggagccga ggcagtctga tgtgaggtag aggaaggtca tgggttggag
     2101 tataccaaga aaaaagggga tcgggagaag gcgccgaagg ccgccccgcc cttcacagcc
     2161 attcaagaag gtatttccgc actccccacc acagggggcc cctggtgagc cttctgatct
     2221 ccctctcgtc gcggcagggc tggg
//
LOCUS       DASSQV010000022         4364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2120342, whole genome shotgun sequence.
ACCESSION   DASSQV010000022 DASSQV010000000
VERSION     DASSQV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2120342"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VFL04_00405"
     CDS             complement(<1..984)
                     /locus_tag="VFL04_00405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7838187.1"
                     /translation="MRFTDLNLHPDIQRGVEDAGYVDCMPVQETVYPHAFEGQDIYAQ
                     SQTGTGKTAAFLLSIFQRIMTVPELRTRKVLILAPTRELAVQIVDEAEKLGKYLPITT
                     GAFYGGVGYGPQMDMLRANVQMIIGTPGRIIDLLQQGKMLLKEVGFLVIDEADRMFDM
                     GFIDDLRRLLRYLPPARDRQTFLFSATLGMRVKDLAWEYMVNPKEIVIEAEHVTVDLV
                     TQELYHVGNHEKFRLLLGIMKREQPKSALIFCNQKFMVEDVAKRLRINGIECDFIMGD
                     LPQTKRQEIIDRLKSGQLEILVATDVAARGLDVAALDLVVNYDLPEDPESYV"
     gene            complement(1041..3245)
                     /locus_tag="VFL04_00410"
     CDS             complement(1041..3245)
                     /locus_tag="VFL04_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7838188.1"
                     /translation="MEAGLNLGRRPHALAALILVLLATARLGADVNTGAIRVTLDANY
                     PPYCFRDEKGVPQGILVDQWKLWEERTGIPVQLMALDWSQAQAAMAEGRADVIDTIFS
                     TPERAKLYDFTPPYATIEVPVFFDRDLSGFGSISGLRGFTVGVKAGDAAIDVLKSRGI
                     ADLRIYPNYESIVRAAVAGEIHVFSIDKPPALYYLYKLGVQARFRSSISLYTGQFRRA
                     VPKGDNARYAQIMRGFAMITKQEYAAIDLKWLGLGLELPQYLRMVLLSLLGGAGLALI
                     LAVWIFLLRRLVRTRTSELSAAVEELTRANQAADRARVEAERASLVKGQFLSNMSHEI
                     RTPLNGIIGMASLLEASPLSGEQTEYLAMMKVSARLLLNIVNDILDLAKIESGMRNLR
                     PEPVALEELARSILLSFGPEAEEKGIALSYEAGPGLPGRAIVDGTALSQVAINLVGNA
                     VKFTDSGRVVLSLSCRAAEGAGTELILKVEDTGIGIPEDRLAEVFERFSQLEEPMTKK
                     RGGTGLGLAIVRELVALMGGRVSATSALGRGSAFTVSLPVVPVAAEAGPASDSAGARA
                     AEDSAAAASEGRSVLAAQAEAGPGSRPAAPGGGPKQRLGAGRRILVVEDNQINLLYLK
                     AVLLREGYAVELASNGREAVEKAAAARFALILMDIQMPEMDGIEALARIRASGGLSLS
                     TTVLALTGYAMEEDQRLLLERGFAAVVTKPIRERQLLRLVDEAAGRDGAAAP"
     gene            3305..3883
                     /locus_tag="VFL04_00415"
     CDS             3305..3883
                     /locus_tag="VFL04_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET7838189.1"
                     /translation="MFGSVCVFCGSSDGLRPAYLEAARAMGRELARRGIRLVYGGGDI
                     GCMGALSRAALAAGGEVLGIIPKRLFERVATAELSELIVASDMHDRKARMYGAADAFV
                     ALPGGVGTMDELFEAWTWRQLGYHQKPVGILEVEGYFAPFLSLIERISAEGFMNAGLV
                     ADLVVDTEPSMLLDRRELAPPIAHHTLPEARG"
     gene            3994..>4364
                     /locus_tag="VFL04_00420"
     CDS             3994..>4364
                     /locus_tag="VFL04_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838190.1"
                     /translation="MAESPAPTPASPIRLSGLLHALLNAAGFVLYLAQDNLAGGGGLL
                     GGGARLGPAAASLAALAILACFVALETLSRPRLLAWLTLACLGSGLAMASCLGLLVDV
                     RWFGYAFILAFQYFTLFVRRYR"
BASE COUNT          652 a         1452 c         1534 g          726 t
ORIGIN      
        1 gacgtaggac tcggggtcct cggggaggtc gtagttgacg accaggtcga gggcggcgac
       61 gtcgaggccg cgggcagcca cgtcggtggc gacgaggatc tcgagctggc ccgacttgag
      121 gcggtcgatg atctcctggc gcttggtctg gggcaggtcg cccatgatga agtcgcactc
      181 gataccgttg atccgcagcc gcttggcgac gtcctcgacc atgaacttct ggttgcagaa
      241 gatgagggcg cttttgggct gctcgcgctt catgatgccc agaaggaggc ggaacttctc
      301 gtggttgccc acgtggtaga gctcctgggt cacgaggtcg accgtcacgt gctcggcctc
      361 gatgacgatc tccttggggt tgaccatgta ctcccaggcg aggtccttca cccgcatgcc
      421 gagggtggcc gagaagagga aggtctggcg atcgcgggcg ggcgggaggt agcgcaggag
      481 gcggcggagg tcgtcaatga agcccatgtc gaacatccgg tcggcctcgt cgatgacgag
      541 gaagccgacc tccttgagga gcatcttgcc ctgctggagc aggtcgatga tgcggcccgg
      601 ggtcccgatg atcatctgga cgttggccct gagcatgtcc atctgggggc cgtagccgac
      661 cccgccgtag aaggcgccgg tggtgatggg caggtacttg ccgagcttct cggcctcgtc
      721 cacgatctgg acggcgagct cgcgggtggg cgccaggatg aggaccttcc tggtccggag
      781 ctcgggcacc gtcatgatcc gctggaatat cgaaaggagg aaggccgcgg tcttccccgt
      841 ccccgtctgg gactgcgcgt agatgtcctg gccctcgaag gcgtgggggt agacggtctc
      901 ctggaccggc atgcagtcca cgtagcccgc gtcctcgacg ccgcgctgga tatcaggatg
      961 aaggttcagg tcggtaaatc gcatgcatac actcgatgga gaaaggttag cttcacaata
     1021 cccgatttgc gccctcgcat ctacggggcc gcagcgccgt cgcggcccgc cgcctcgtcg
     1081 acgaggcgga ggagctggcg ctccctgatg ggtttggtga cgacggcggc gaaaccccgc
     1141 tcgaggagga ggcgctggtc ctcctccatg gcgtagcccg tcagggcgag gacggtggtg
     1201 gagagcgaga gcccgcccga ggcgcggatc ctggccaggg cctcgatccc gtccatctcg
     1261 ggcatctgga tgtccatgag gatgagggcg aaacgggcgg cggcggcctt ctccacggcc
     1321 tcccgcccgt tggaggcgag ctcgacggcg tagccctccc tgaggaggac ggccttgaga
     1381 tagaggaggt tgatctggtt gtcctccacg acgaggatgc gccttccggc ccccagcctc
     1441 tgcttcggcc ctccaccagg cgcggctggc ctcgaccccg gtccggcttc ggcctgggcg
     1501 gccagcaccg agcggccctc ggaagctgcg gccgcggagt cctcggcggc ccgcgccccc
     1561 gccgaatccg atgccgggcc cgcctcggcc gcgaccggga ccacgggcag gctcacggtg
     1621 aaggccgagc cccggccgag ggcgctcgtc gccgacaccc tgccacccat cagggccacg
     1681 agctcgcgga cgatcgcgag gcccaggccg gtcccgcccc gcttcttggt catcggctcc
     1741 tcgagctggc tgaaacgctc gaagacctcg gccagcctat cctcggggat cccgatgccc
     1801 gtgtcctcga ccttgaggat gagctcggtc ccagccccct ccgcggcccg gcaggacagg
     1861 gagagcacga ccctgcccga gtcggtgaac ttgacggcgt tgcccacgag gttgatggcg
     1921 acctggctca gggccgtccc gtcgacgatg gcccgaccgg gcaggccggg gccggcctcg
     1981 taggacaggg ctatgccctt ctcctcggcc tcggggccga aggagaggag gatcgagcgg
     2041 gcgagctcct cgagggccac gggctcgggc ctgaggttgc gcatgcctga ctcgatcttc
     2101 gcgaggtcga ggatgtcgtt gacgatgttg agcaggagcc tggccgagac cttcatcatc
     2161 gccaggtact cggtctgctc ccccgagagg ggcgaggcct cgaggaggct cgccatgccg
     2221 atgatgccgt tgaggggggt cctgatctcg tggctcatgt tggaaaggaa ctggcccttg
     2281 accagggacg ctcgctcagc ctcgacgcgg gcgcggtcgg cggcctggtt ggcccgggtg
     2341 agctcctcga cggcggcact gagctcgctg gtcctcgtgc gcacgagccg cctgaggagg
     2401 aagatccaca cggccaggat cagggcgagg ccggcccccc cgaggaggct gaggaggacc
     2461 atcctgaggt actggggcag ctccaggccc aggccgagcc acttgaggtc gatggcggcg
     2521 tattcctgtt tcgtgatcat ggcgaagccg cgcatgatct gggcgtagcg ggcattgtcg
     2581 cccttgggga ccgcccgtct gaactgcccc gtgtacaggg agatcgagga gcggaacctc
     2641 gcctggaccc cgagcttgta gaggtagtac agggccgggg gcttgtcgat gctgaagacg
     2701 tggatctcgc cggccacggc cgccctgacg atggactcgt agttggggta gatcctgagg
     2761 tcggcgatgc ccctcgactt caggacatcg atagcggcgt cgccagcctt gaccccgacg
     2821 gtgaagccgc gcaggcccga gatgctcccg aagccgctca ggtcccggtc gaagaagacc
     2881 ggcacctcga tggtggcgta gggaggggtg aagtcgtaga gcttggcccg ctcgggggtg
     2941 gagaagatcg tgtcgatgac gtcggcccgg ccctcggcca tcgccgcctg ggcctgggac
     3001 cagtcgaggg ccatgagctg gacggggatg ccggtgcgct cctcccagag cttccactgg
     3061 tcgacgagga tgccctgggg gacgcccttc tcgtcgcgga agcagtaggg gggatagttg
     3121 gcgtcgaggg tgacccgtat ggcgcccgtg ttcacgtcgg ccccaagccg ggccgtggcg
     3181 aggaggacaa ggatcagtgc ggcgagcgcg tgaggtcttc tgcccaagtt gagtcccgct
     3241 tccacgaatc tagcctatcg cggatggcct gcctagtcgg gtcattcggg tgtagaatcg
     3301 ggccatgttc ggatcggtct gcgtcttctg cggctcgagc gacggcctca ggcccgccta
     3361 cctcgaggcg gcccgggcga tggggaggga gctcgcccgc cggggcatac gcctcgtcta
     3421 cggcgggggc gacataggct gcatgggggc cctctcccga gccgccctgg ccgccggggg
     3481 cgaggtcctc ggcatcatcc ccaaacggct cttcgagcgg gtagcgaccg ccgagttgag
     3541 cgagctcatc gtcgccagcg acatgcacga caggaaggcc aggatgtacg gggcggccga
     3601 cgccttcgtg gccctgcccg gcggggtcgg caccatggac gagctcttcg aggcctggac
     3661 ctggcgccag ctcggctacc accagaagcc cgtcggcatc ctcgaggtcg agggctactt
     3721 cgcccccttc ctctccctga tcgagcggat ctcggccgag ggcttcatga acgccggcct
     3781 agtagccgac ctcgtggtcg acaccgagcc ctccatgctc cttgaccgcc gcgagctcgc
     3841 cccgcccatc gcccaccaca cgctccccga agcgcggggc tgagggccac ggcctcgacg
     3901 ggggcggggc ctgcgccttg acagggcccc gagcccacac cgagcccccg gccgggctcg
     3961 aagcccccgt cgatctgata tacaatttat aacatggcag aaagccccgc cccgaccccg
     4021 gcctccccga tccggctctc gggcctcctc catgccctgc tgaacgcggc gggcttcgtc
     4081 ctctacctgg cccaggacaa tctggcagga ggcgggggcc tcctcggcgg gggagccagg
     4141 ctcggccccg cggctgcctc cctggcagcg ctggccatcc tcgcctgctt cgtggccctc
     4201 gagaccctgt cccgcccgag gctcctcgcc tggctgaccc tcgcctgcct cggatccggc
     4261 ctcgccatgg cctcatgcct cggccttctc gtcgacgtca ggtggttcgg ctacgccttc
     4321 atcctcgcct tccagtactt cacccttttc gtcaggcgct accg
//
LOCUS       DASSQV010000023         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2120981, whole genome shotgun sequence.
ACCESSION   DASSQV010000023 DASSQV010000000
VERSION     DASSQV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2120981"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VFL04_00425"
     CDS             complement(<1..709)
                     /locus_tag="VFL04_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HET7838191.1"
                     /translation="MDSQASVSRISILGVPVDTLPEDRIDEIVAKFEDGKNHQIILLS
                     VWDLMKARREGEFRTMVAGASLVLPISLSIVKGAHFLRKPEPVRYMPFDFAIKLLAAL
                     ERRGKSLYILGSSRSSVQKAEANIKSTFPGLRVVGRFAGHYPRHMEGAIIEAVRKATP
                     NLLMVGSGVPGRERWIPRNLSHFNAGIYLWCSDLFEVFAEKRAKPSRQLFAKGLEWIP
                     YLIRRPWNLYRILTILHF"
     gene            complement(688..1533)
                     /locus_tag="VFL04_00430"
     CDS             complement(688..1533)
                     /locus_tag="VFL04_00430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HET7838192.1"
                     /translation="MRQFLIPEEWEGGPTCEIAGGRARYLTRVLRLVAGDEFPGLDPR
                     GRRWLCRVGEAGQDRVILELSALPPTFEASGLEDVRGGRGSVAAPSAPPAQPGPLPPI
                     VLVQGLPKGAKMDLIVRQAAEAGLAAVLPLLAERSVAREGREAEAKRARWERIAREAI
                     QQSGSARPTAVAPITRLAELPVWLAQPPRGQGRVLKLLLHEAPLAQASLHGYLTEAPD
                     EIVLCVGPEGGFAPGETDFLLASGFLPLRLAGAILRTETAALYAVAAVETIISERSSW
                     IPKPQ"
     gene            complement(1577..3094)
                     /locus_tag="VFL04_00435"
     CDS             complement(1577..3094)
                     /locus_tag="VFL04_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET7838193.1"
                     /translation="MTQSTFVPRRHRIAWGAAAIVLIAISMVSVPVPRAFAQQNDQAS
                     ADARKYLELLNNTFRFVLQSYVDQPDPKKLYEGAMKGIFESLGDPYSVFLDEQMMSDM
                     TELTSGQFGGVGLYISKQLRDPKKPEDEPRYIEIVSPIEDTPGWKYGLKAGDLIIKID
                     GETTAPLSTDEAQKRIRGKPDSNVTLTIRRPGLEDFDATIGRARIEVPTVKHALIPTP
                     KGNVGYLRIIEFTAQTKARVEDSIRDFEKTGYRALVIDVRNDPGGLLQSVIDVANYFI
                     DSGVIVSTKSRNAYENSVARAKPGIAISGSKPIVILLNKGSASASEILAGALKDNKRA
                     YLVGENSYGKGSVQQIYPIDGTGFKITIARYYTPSDENIDKTGIPPDLEAKEPDLSKA
                     ETDSLQKLFDSGRISEISSSWASKTAAEKTAVSKEFGLPENLLARLAKDEANRKSPKP
                     PVYDLEYDVALKAALDVIDRPDYQQLLAATKTVKELVEAKKAAQALAAPAPAAKP"
BASE COUNT          488 a          963 c         1163 g          583 t
ORIGIN      
        1 tgaagtggag gatcgtgagg atgcggtaga ggttccaggg ccgcctgatg aggtagggga
       61 tccactcgag gcccttcgcg aagagctggc gcgagggctt ggcccgcttc tcggcgaaga
      121 cctcgaaaag gtcggagcac cagagataga tgccggcgtt gaagtgcgag aggttccgcg
      181 ggatccagcg ctccctcccg gggaccccag agcccaccat gaggaggttc ggggtggcct
      241 tcctgaccgc ctcgatgata gccccctcca tgtgtctggg gtagtgcccg gcgaagcggc
      301 cgaccacgcg gaggccggga aaggtcgact ttatgttggc ctcggccttc tggacgctcg
      361 agcgggagga gccaaggatg taaagggact tgcccctccg ctccagggcg gcgaggagct
      421 tgatcgcgaa gtcgaagggc atgtatctga cgggctcggg tttcctgagg aagtgagcgc
      481 ccttgacgat ggagagggag atggggagga caaggctcgc cccggcgacc atcgtgcgga
      541 actcgccttc cctgcgggcc ttcatgaggt cccagaccga gagcaggatg atctggtggt
      601 tcttgccgtc ctcgaacttg gccacgatct cgtcgatgcg gtcttccggg agggtgtcga
      661 ccggcacgcc cagtattgag atgcggctca ctgaggcttg ggaatccatg acgagcgctc
      721 cgatatgatg gtttcgaccg cggcgacagc gtagagcgcg gccgtctcgg ttctgagtat
      781 cgcaccggca agcctgagcg gaaggaaacc cgaagccagc aggaaatccg tctcgccggg
      841 ggcgaatccg ccctccggtc ccacgcaaag gacgatctcg tcgggtgctt cggtaagata
      901 cccatggagg ctcgcctgcg caaggggagc ctcgtggagg aggagtttga ggactcggcc
      961 ctggccccgg ggcggttggg ccagccagac ggggagctcg gcgagccgcg ttatgggcgc
     1021 gactgccgtg ggccgggccg agcccgactg ctggatcgcc tccctcgcga tgcgctccca
     1081 gcgcgcccgc ttggcctcgg cctcccggcc ctccctggcg accgagcgct cggcgaggag
     1141 ggggaggacg gccgcgaggc cggcctcggc ggcttggcgg acgatcaggt ccatcttggc
     1201 ccccttggga aggccctgga cgaggacgat ggggggcagg ggccctggct gggcgggtgg
     1261 agccgagggg gcggcgaccg aaccacggcc gcccctcacg tcctcgaggc ccgaggcttc
     1321 gaaggtgggg ggaagggcgg acagctcgag gattacccga tcctggccgg cctcgccgac
     1381 gcggcagagc cagcgcctcc cccgcgggtc caggccgggg aactcgtctc cagcgacgag
     1441 gcggaggacc cgggtcaggt atcgcgccct cccccccgcg atctcgcagg tcgggccgcc
     1501 ctcccattcc tcggggatca ggaactgccg catcgccggt cctgtgtgtc gggggagggc
     1561 tcctcccccg ctggcttcag ggcttcgcgg ccggggccgg ggccgcgagg gcctgggctg
     1621 cctttttggc ctcgaccagc tccttcacgg tcttcgtggc ggcgaggagc tgctggtaat
     1681 cgggcctgtc gataacgtcc agggcggcct tgagggccac gtcgtactcc aggtcataga
     1741 cggggggctt ggggctcttc cggttggcct cgtccttcgc tagacgggcg aggaggttct
     1801 cgggcaggcc gaactccttg gagacggcgg tcttctcggc agcagtcttg ctggcccagc
     1861 tgctcgagat ctcggagatc ctgcccgagt cgaagagctt ctggagggag tcggtctcgg
     1921 ccttcgagag gtcgggctcc ttcgcctcga ggtcgggagg gatcccggtc ttgtcgatgt
     1981 tctcgtcgct cggggtgtag tagcgggcga tggtgatctt gaagcccgtg ccgtcgatcg
     2041 ggtagatctg ctggaccgag cccttgccgt agctgttctc acccacgagg taggcccgct
     2101 tgttgtcctt gagggcaccg gccaggatct cggaggccga ggccgagccc ttgttgagga
     2161 ggatgacgat gggcttgctg cccgagatgg cgatccccgg cttggccctg gccacggagt
     2221 tctcgtaggc gttgcggctt ttggtcgaga cgatgacgcc cgaatcgatg aagtagttgg
     2281 cgacgtcgat gaccgactgg aggaggccgc ccggatcgtt gcggacgtcg atcacgaggg
     2341 cgcggtagcc cgtcttctcg aagtcgcgga tggagtcctc gaccctcgcc ttggtctggg
     2401 cagtgaactc gatgatcctg aggtagccca cgttcccctt gggcgtgggg atgagggcgt
     2461 gcttgaccgt cggcacctcg atcctcgcgc ggccgatggt ggcgtcgaag tcctcgagcc
     2521 cggggcggcg gatcgtcagg gtcacgttgc tgtcgggctt gccgcggatg cgcttctgag
     2581 cctcgtctgt ggagaggggg gccgtggtct cgccgtcgat cttgatgatg aggtccccgg
     2641 ccttgagacc gtatttccag cccggcgtgt cctcgatcgg ggacacgatc tcgatgtagc
     2701 gcggctcgtc ctcgggcttc ttcgggtccc gcagctgctt ggagatgtag aggcccacgc
     2761 ccccgaactg gccggaggtg agctcggtca tgtcgctcat catctgttcg tcgaggaaga
     2821 ccgaataggg gtcgccgagg gactcgaaga tccccttcat cgcgccctcg tagagcttct
     2881 tggggtcggg ttggtcgaca tagctctgca gcacgaagcg gaaggtgttg ttgagcagct
     2941 cgaggtactt gcgcgcgtcg gccgaggcct ggtcgttctg ctgggcgaag gccctgggca
     3001 cggggaccga gaccatggag atggcgatca ggacgatcgc cgccgccccc caggcgatgc
     3061 ggtgcctgcg aggaacaaat gtgctttgcg tcataaaata caatatccat tcggccgccc
     3121 ccctctgtca accttgcccg cgcttgactg aggcaggggc tgaaagtaca cttcgcggca
     3181 tatatgaagc gaaaggc
//
LOCUS       DASSQV010000024         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1250939, whole genome shotgun sequence.
ACCESSION   DASSQV010000024 DASSQV010000000
VERSION     DASSQV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250939"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1872
                     /locus_tag="VFL04_00440"
     CDS             <1..1872
                     /locus_tag="VFL04_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7838194.1"
                     /translation="YSLKAECYCLHVETGAALGEGDKDRLERNLNLARSLGARVQSVP
                     NVDVADGVLTYARRNEVSIIVVGKRGIAPARRIFAGPSLTERIVKESGDIAVFAVQDR
                     PIREPLRSRVAALVKASPPWQYAAALLGIAAVTGLNLVLAGTVGYWAASIPYLAAISL
                     LALVLERRPVLVAALVSAVLWDVLFIPPRYAIVISRPEDYLMLGLYVILAATSGWATG
                     KLKANQAMLLVREERMSLLSELASELAATSGAEAIVAKAVGFLERAFQSEALIILKGP
                     DGALKAEPEGGWMPLDEKATSAAHYCLSSGRAAGRYTSTLPVVEWHFEPLDSPKGGIG
                     VIGLRPATGQAWTAQLESLLRTMSRTVSLAVERALLAAEKEAAALDRESERLGRLLLD
                     SVSHELRTPLTVIEGSASALADEETAGDPAARGALVAGILGASGRLNGIVENLLSMSR
                     LEAGKLVLARSLVEAEELVAAAAKLAEADLAGHELSLKPEPLSISCDAGLIIQALVNL
                     LRNAARHGGSRSRIALSVVPTASGAEFTVADDGPGLGPAELPRLFTKFFRAEGAAPGG
                     SGLGLAICKGIVEAHGGRIAAGNLAGGGFAVSFTLPAGEDCPDPDGLVARAGEGGGP"
BASE COUNT          275 a          761 c          681 g          284 t
ORIGIN      
        1 tacagcctca aggccgagtg ctactgcctc cacgtcgaga cgggagccgc cctgggcgag
       61 ggcgacaagg accgccttga gcggaacctg aacctggccc ggagcctcgg cgcccgcgtc
      121 cagagcgtcc cgaacgtcga cgtggccgat ggggtcctga cctacgcccg gaggaacgag
      181 gtctcgatca tcgtcgtggg caagcggggc atcgccccgg cgaggcgcat cttcgccggc
      241 ccatcgctca ccgagcgcat cgtcaaggag tcgggcgaca tagccgtctt cgccgtccag
      301 gacaggccga tccgcgagcc cctgcgcagc cgggtggccg ccctggtcaa ggcctcgcct
      361 ccctggcagt acgcggccgc cctcctcggc atcgccgccg tgaccggcct caacctcgtc
      421 ctcgccggca ccgtgggcta ttgggccgcc tccataccct acctggcggc catcagcctc
      481 ctcgccctcg tcctcgagcg gaggcccgtc ctcgtggccg ccctcgtctc ggcggtcctc
      541 tgggacgtcc tcttcatccc gccgcgctac gccatcgtca tatcgaggcc cgaggactac
      601 ctgatgctcg gcctctacgt catcctcgcc gcgacctcgg gatgggccac gggcaagctc
      661 aaggccaacc aggccatgct cctcgtgcgc gaggagagga tgagcctcct ctccgagctg
      721 gcctcggagc tcgccgccac ctcgggcgca gaggccatcg tggccaaggc cgtcggcttc
      781 ctcgagaggg ccttccagtc cgaggccctc ataatcctca aggggcccga cggggccctc
      841 aaggccgagc ccgagggcgg atggatgccc ctggacgaga aggccacgtc cgcggcccac
      901 tactgcctct cctcgggccg ggccgctggc aggtacacct cgacccttcc cgtcgtcgaa
      961 tggcatttcg agcccctgga ctcgcccaag ggcgggatcg gggtgatcgg cctgaggccg
     1021 gccacgggcc aggcctggac cgcccagctc gagtccctcc tgcggacgat gtcgcgcacc
     1081 gtctcactcg ctgtggagcg ggccctcctc gccgcggaga aggaggccgc ggccctcgac
     1141 cgcgagtccg agcggctcgg caggctcctc ctggactcgg tctcccacga gctgcggacc
     1201 cccctgaccg tgatcgaggg gagcgcctcg gccctggccg acgaggagac ggccggcgac
     1261 ccggccgcgc ggggcgccct cgtggccggg atcctcggcg cctcgggccg gctcaacggc
     1321 atcgtcgaga acctgctctc gatgagcagg ctcgaggcgg gcaagctcgt cctcgcgcgc
     1381 agcctggtcg aggccgagga gctcgtggcc gccgccgcga agctcgccga agccgacctc
     1441 gcgggacacg agctctcgct caagcccgag cccctgtcga tctcctgcga cgccggcctc
     1501 atcatccagg ccctcgtgaa cctgctcagg aacgcggcgc ggcacggagg ctcgaggtcg
     1561 cgcatcgcgc tctccgtggt cccgacggcg tccggggccg agttcaccgt cgccgacgac
     1621 ggcccgggcc tgggcccggc cgagctgccg aggcttttca ccaagttctt ccgcgccgag
     1681 ggggcggccc ccggcggctc gggcctgggc ctcgcgatct gcaagggcat cgtcgaggcc
     1741 cacggcggcc gcatcgcggc ggggaacctc gcgggcggcg gcttcgccgt gtccttcacc
     1801 ctgcccgccg gcgaggactg ccccgatccc gatggccttg tggcccgcgc tggggaaggg
     1861 ggcgggccat gaagcgggtc ctcgtcatcg acgacgagcc ccagatcagg aggctcctca
     1921 agatcagcct cgagaagaag ggctacgagg tctgcgaggc cgcgacgggc ttcgagtgca
     1981 tccaggccgt ccagggcgtc c
//
LOCUS       DASSQV010000025         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_629674, whole genome shotgun sequence.
ACCESSION   DASSQV010000025 DASSQV010000000
VERSION     DASSQV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629674"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VFL04_00445"
     CDS             complement(<1..1091)
                     /locus_tag="VFL04_00445"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET7838195.1"
                     /translation="MRFSARDLAVTAVVLILSSLLVVLYVQERGRTTYRSGEKALGTI
                     VFKKLNATRRAQSALVWERIRNNSPVYAADTLRTADASEAEVCFDDGTSLDMYENSML
                     RLEFKGKMRTLEFLGGDISVSGSKGKGGSELSGLAPEAGGTGSGYTISAGGKTIALSE
                     DSRATLSRTGSTLSVAVSEGQVGVTDSLGASETLDRSKELHVNLETGISQVLARPILP
                     LFPEQNARLLLERVGKANLRFAWEGEGQASLELSQTRDFERPAVSARAGGGSLELGVE
                     PGTWYWRLRSEDGSTSPEQRFTLYAEQPPQPILPASGAELSYRKILPAVRFSWTRMES
                     AAAYVFEVASDPGFAKPLRRDRTSMEAITV"
     gene            complement(1072..>2356)
                     /locus_tag="VFL04_00450"
     CDS             complement(1072..>2356)
                     /locus_tag="VFL04_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7838196.1"
                     /translation="LQSIVQTGAASLTYAVGFDGELIAHPDFEQVRLGANYQGNQLVA
                     KLLSSPMDNMQVRYDDSTGAPNLGAFRKLGIGQVSVTTSVPVSRVYEAALTVTRQNLY
                     LGGVVLLFSILAVWFFSRSMTRPVMDLVSASRRIEAGEFEIDLVPSTKDELGLLTESF
                     VEMGRGLAERERVKETFGKFVNKEIAERALKGSLELGGTRKTATILFSDIRSFTAISE
                     KLAPEAVVEFLNAYMTRMVECIEATGGVVDKFIGDAIMAVWGAPLSKGSAGEDALQAV
                     RAMFMMRDSLIEFNRDRGGPDKPLIRIGCGVNTGPCLAGQIGSLQRMEYTVIGDAVNL
                     ASRIEALNKPFGTDILVSESTYELVKQEVRAEPMPAIKVKGKAAALSIYGLVGFRDGP
                     GPKSLDEVRSLLGIPRPEGRVDVDKEETKYEILGT"
BASE COUNT          366 a          783 c          806 g          401 t
ORIGIN      
        1 accgtaatgg cctccatgct tgtcctgtcc cggcgcaggg gcttcgcgaa gccaggatcc
       61 gaggcgacct cgaagacata ggcagcggcg ctctccatgc gcgtccagga gaagcgcacc
      121 gcgggaagga ttttccggta gctcagctcg gcgcccgagg ccggcaggat gggctggggc
      181 ggctgctcgg cgtatagggt gaagcgctgc tccgggctgg tagacccgtc ctcggatctg
      241 agcctccagt accaggtgcc tggctcgacg ccaagctcga gggagccacc tccggcccgg
      301 gccgagaccg ctggccgctc gaagtccctg gtctgggaca gctcgaggct cgcctgaccc
      361 tcgccctccc atgcgaagcg cagattcgcc ttccccacgc gctcaaggag gaggcgggcg
      421 ttctgctcgg ggaagagggg caggatggga cgggccagga cctgggagat ccccgtctcg
      481 aggttgacgt ggagctcctt cgagcggtcg agggtttcgc tggccccgag cgaatcggtc
      541 accccgacct ggccctcgga gaccgcgacc gaaagggtgc tgcccgtccg gctcagcgtg
      601 gcgcgggaat cctcggagag cgcgatggtc ttgccccccg cgctgatcgt atagccggaa
      661 cctgtcccgc cggcctcggg cgcgaggccc gagagctcgg atccgccctt ccccttggag
      721 cccgagaccg agatgtcccc gccgaggaac tcgagggtcc tcatctttcc cttgaactcg
      781 agcctgagca tgctgttctc gtacatgtcc aggctggtcc cgtcgtcgaa gcagacctcg
      841 gcctcggagg cgtcggccgt cctgagggtg tcggcggcgt agaccgggct gttgttcctg
      901 atgcgctccc agaccagggc gctctgggcc cgccgggtgg cgttgagctt cttaaagacg
      961 atcgtgccca gggccttctc cccggacctg tacgttgtgc gacctcgctc ctgcacgtag
     1021 aggacgacga ggagcgacga gaggatgagg acgacggcgg tcaccgcgag gtcacgtgcc
     1081 gagaatctca tacttcgtct cttccttgtc cacgtcgacg cggccctccg gccgcgggat
     1141 gccgaggagg gagcggacct cgtccaggct cttcgggccg ggaccgtccc tgaagcccac
     1201 gaggccatag atggagaggg ccgcggcctt gcccttgacc ttgatggcgg gcatcggctc
     1261 ggcccggacc tcctgtttca cgagctcgta ggtggactcg gagacgagga tgtcggtgcc
     1321 gaagggcttg ttgagggcct cgatgcggct ggcgaggttc acggcgtcgc ctatcaccgt
     1381 gtactccatc cgctgcagcg agccgatctg tccggcgaga cagggccccg tgttcacccc
     1441 gcagccgatc cggatcaggg gcttgtccgg accgccccgg tcccggttga actcgatgag
     1501 ggagtcgcgc atcatgaaca tcgcgcgcac ggcctgaagg gcgtcctcgc cggccgagcc
     1561 cttggacagg ggggctcccc agacggccat gatggcgtcc ccgatgaact tgtccaccac
     1621 cccgcccgtg gcctcgatgc actccaccat cctcgtcata tacgcattca ggaactccac
     1681 cacggcctcc ggcgcgagct tctccgagat cgctgtgaag gacctgatgt cggagaagag
     1741 gatggtggcc gtcttgcggg tgccgcccag ctcgaggctt cccttgaggg cgcgctcggc
     1801 gatctccttg ttcacgaact tgccgaaggt ctccttgact cgctcgcgct cagcgaggcc
     1861 cctgcccatc tcgacgaagc tctcggtgag caggcccagc tcgtccttcg tggagggcac
     1921 gaggtcgatc tcgaactcgc ccgcctcgat ccgcctcgag gccgagacga ggtccatgac
     1981 cggccgggtc atggagcggg agaagaacca gaccgcgagg atcgagaaga ggaggaccac
     2041 cccgcccagg tagaggttct gccgggtcac cgtcagggcc gcctcgtaga cgcggctcac
     2101 cgggacgctc gtggtcacgc tcacctggcc gatgccgagc ttgcggaagg ccccgaggtt
     2161 cggggccccc gtcgagtcgt cgtagcggac ctgcatgttg tccatggggc tcgagaggag
     2221 cttggccacg agctggttgc cctggtaatt ggcgcccagc ctcacctgct cgaaatcggg
     2281 atgggcgatg agctcgccgt cgaagccgac ggcgtaggtg aggctcgccg ccccggtctg
     2341 gacgatggac tggagg
//
LOCUS       DASSQV010000026         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_879241, whole genome shotgun sequence.
ACCESSION   DASSQV010000026 DASSQV010000000
VERSION     DASSQV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879241"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFL04_00455"
     CDS             <1..655
                     /locus_tag="VFL04_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET7838197.1"
                     /translation="GNAFQNLEPMCLTRPDCISVDENIDLAAAKEVTDRHGICLGGNI
                     QLTVVMLFGSQADNMKAVVDIVDSCGTGNLVVSPGCDMPYDLPVENVVAAGQAVRETA
                     QVRGILEHYQGARIELEVELPDYARLERPLVEVFTLDSATCPACGYMMEAAREAREAF
                     GPAIDLVEYKFTVKENIARCMKLGVAKLPSIYLNGELAYSSIIPSREELMGKIRGLL"
     gene            652..1407
                     /locus_tag="VFL04_00460"
     CDS             652..1407
                     /locus_tag="VFL04_00460"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HET7838198.1"
                     /translation="MSGHRAAYKLISCSVFQRELCAAIAVSPNVVDPEFLELGLHERP
                     ASLRARLQERIDAAGAASDGPRYEAILLGYGLCGNALAGIEARGLPLVLARAHDCCTI
                     LLGSRSAFLAQFGECLSASWSSAGYIERGSTYFRTSELGRAIGHGLEYDELVASYGED
                     NANYIYETLHPSLEEKELFYIETPETEGLGYAEAMRARAAAEGRDFVLLRGSTRLLRA
                     LLAGNWEDEDFLVVPPGRRIAALYDHERVLALE"
     gene            1417..>2091
                     /locus_tag="VFL04_00465"
     CDS             1417..>2091
                     /locus_tag="VFL04_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7838199.1"
                     /translation="MRMYFVGGFLGSGKTTAIAGACRRLIDRGRRVGVVTNDQGMLQV
                     DGLFMRAAGLPAVELGGGCLCCRYEEFETRLAGLVERERPDAVFAESVGSCADLVATV
                     VKPFEAFRAGHGGPSVFSVLADSRLLLARLSGRRLPFSEDVVYIFDRQLEEAELLVLN
                     KRDLLSPAELEGLEALADRAWPGKPRLALSASTEAGAAEWLAALEAWEGGAGRDSLEI
                     DYARYGS"
BASE COUNT          310 a          757 c          701 g          323 t
ORIGIN      
        1 cggcaacgcc ttccagaacc tcgagcccat gtgccttacc cgtcccgact gcatctccgt
       61 ggacgagaac atcgacctgg ccgccgcaaa ggaagtgacc gatcgccacg ggatctgttt
      121 gggcggcaac atccagctca cggtcgtcat gctcttcggg agccaggccg acaacatgaa
      181 ggcggtggtg gacatcgtgg actcctgcgg gaccgggaac ctcgtcgtct ccccgggctg
      241 cgacatgccc tacgacctgc ccgtggagaa cgtggtcgcc gccggccagg ccgtccgcga
      301 gacggcccag gtccgcggca tcctcgagca ctaccagggg gcgcggatcg agctcgaggt
      361 cgagctgccc gactacgcgc ggctggagcg ccccctcgtc gaggtcttca ccctcgactc
      421 cgccacctgc cccgcctgcg gctacatgat ggaggcggcc cgcgaggcgc gggaggcctt
      481 cggcccggcc atcgacctcg tcgagtacaa gttcaccgtg aaggagaaca tcgctcgctg
      541 catgaagctc ggggtggcga agctcccgtc catctatctg aacggcgagc tggcctactc
      601 ctcgatcatc ccctcgaggg aggagctcat ggggaagatc cggggtctgc tgtgagcgga
      661 caccgggcgg cgtacaagct catctcatgc agcgtgttcc agcgcgagct ctgcgccgcg
      721 atcgcggtgt cgcccaacgt ggtcgatccg gaattcctcg agctcggcct ccacgagcgg
      781 ccggcctccc tccgcgcgag gctccaggag cgcatcgacg cagccggcgc ggcttcagac
      841 gggccacgct acgaggccat cctactcggc tacgggctct gcggcaacgc cctcgcggga
      901 atcgaggcca ggggcctgcc cctcgtcctc gcgagggccc acgactgctg caccatcctc
      961 ctcggctcgc gctccgcctt cctcgcgcag ttcggcgaat gcctctcggc ctcgtggagc
     1021 tcggcgggct atatcgagcg gggctcgacc tatttcagga cctctgagct cggccgggcc
     1081 attggccacg gcctcgagta cgacgagctc gtggccagct acggcgagga caacgcgaac
     1141 tacatctacg agaccctcca cccgagcctc gaggagaagg agctgttcta catcgagacg
     1201 cccgagaccg agggcctcgg ctacgccgag gccatgcgcg cccgggcagc cgcggagggc
     1261 agggacttcg tcctcctgcg cggctcgaca cgcctccttc gagccctcct cgcggggaac
     1321 tgggaggacg aggacttcct cgtcgtgccc ccgggcagga ggatcgcggc gctctacgac
     1381 cacgagcggg tcctggccct agaatagggg cgcaccatga ggatgtactt cgtcggcggc
     1441 ttcctgggtt cgggcaagac cacggccata gcgggcgcct gccgccgcct catcgaccga
     1501 ggcagacggg tcggggtcgt caccaacgac cagggcatgc tccaggtcga cgggctcttc
     1561 atgcgcgcgg ccggcctccc cgccgtcgag ctgggcgggg gctgtctctg ctgccgctac
     1621 gaggagttcg agacccgtct cgcgggcctc gtcgagcgcg agaggcccga cgcggtcttc
     1681 gccgagtcgg tgggctcctg cgccgacctc gtcgcgacgg tggtgaagcc cttcgaggcc
     1741 ttccgcgcgg gccatggggg gccctccgtg ttctctgtcc tcgccgactc gaggctcctc
     1801 ctcgcccggc tctccggcag acgcctgccg ttcagcgagg acgtggtcta catcttcgac
     1861 cgccagctcg aggaggccga gctcctcgtc ctcaacaaac gcgacctcct cagccccgcc
     1921 gagctcgagg gactcgaggc cctcgccgac cgcgcctggc cgggcaagcc gcggcttgcc
     1981 ttgagcgcct ccaccgaggc gggggcggcc gagtggctcg ccgccctgga ggcctgggag
     2041 ggcggggccg ggcgggactc cctcgagatc gactacgcgc gctacggctc g
//
LOCUS       DASSQV010000027         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2371383, whole genome shotgun sequence.
ACCESSION   DASSQV010000027 DASSQV010000000
VERSION     DASSQV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2371383"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFL04_00470"
     CDS             complement(<1..556)
                     /locus_tag="VFL04_00470"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET7838200.1"
                     /translation="MSNTHPKLGLAAVALAALSLGLSCASSGGGSYGDSRSSESSAAS
                     RARAAGNSSKPAPAPQAPDSHDDGGDSGGGLLFALLFGAKPQTPPPIPSGTLAVIGLP
                     SGASLFIDGNLELVSTIALPVGEHEVRVSRFGYEDFVASSTVFEGTRAELKVELQSAP
                     FSVRGVEASPEAFDPGDPGFLGGCG"
     gene            742..3021
                     /locus_tag="VFL04_00475"
     CDS             742..3021
                     /locus_tag="VFL04_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7838201.1"
                     /translation="MARAKHGPISTLIMLSCGFALALVGVVVLAGWVLGLRILTSLHP
                     AYLPTAPDTALLFVLVGVIVATETRWKWGAGARKAAALLLVLVGLYGGLKFLEGVVGT
                     DLSFAGRLFPVSERLRGFPLRRMSPIAGFLFLLGAAAMILRERAIGRRRTADLVGLLG
                     SIVALLAFVAVLGYLFGTPLLYRGTLIPLAAPSSFAFLLWGTAIVASVGSKARPFRRF
                     VGRAMDARLMRSIVPLTVAFSLGGGLLQSLLAKSPKYDALESSLITLTFLAASAVVVM
                     RASRRVFRRAEAAELEMRRLDDEKRESERKYRRLYEGMRDGYTIADPSGRYLESNAAF
                     RELVGYSDEELRSLSVLGITPEKWHPLQRRIMEEEVLVRGYSELFEKEYRRKDGRVIP
                     VELRIFLIRDEAGGWLGTCSVVRDISERKKAEAGLRESEERFRAIFENAGIGMALVDK
                     EGRITRANASLAAMLDYQLSELEGRSLMELTHPDDRESDWNLYLNLIAGARDSYQIEK
                     RYLRKDGRVIWGQLTASTFCIPSEGCSIIGMVEDVTSRRAQDELIRRSLGEKEALLRE
                     LHHRVKNNLQLITSLLSLQGASLPEGPAGDAVRVALHDATGRVGAMAGIHELVYLSKD
                     FSEIDFGAYMASIARGQLLELGGSRPIRLDLDLEELRISLDDAIPCGLLINEALSNVA
                     KYAYPPDWPGQRRAELSLRRSAGGRRRIVIRDHGVGIQPDQPESRGLGLHIMRILSDQ
                     IGGRLEVRSEDGVEVQLEF"
BASE COUNT          500 a         1012 c         1062 g          511 t
ORIGIN      
        1 tcccgcagcc gccaaggaag ccggggtcgc cgggatcgaa ggcctcgggg ctggcctcga
       61 cccccctcac cgagaagggg gcgctctgga gctcgacctt gagctcggcg cgcgtgccct
      121 cgaagaccgt ggagctggcg acgaaatcct cgtagccgaa acgactcacc ctcacctcgt
      181 gttccccgac gggaagggcg atcgtcgaga cgagctcgag gttgccgtcg atgaagaggc
      241 tcgcccccga gggcaggccg attacggcga gggtgcccga ggggatgggg ggaggagtct
      301 ggggcttggc gccgaagagg agggcgaaga gcaggccacc gcccgaatcg ccgccatcgt
      361 catgcgagtc cggggcctgg ggcgctgggg cgggtttgga gctattgccg gcggcacggg
      421 cccgggaggc cgccgaggac tcggagctgc gggaatctcc gtagcttccg cctcctgaac
      481 tggcgcagga gagaccgagg gacagtgccg cgagagccac ggcggcgagc ccaagcttgg
      541 gatgggtgtt gctcatcgga gacagtatag cgtgggcccg gccccccgtg gtcgcctgtg
      601 tggggctagt ctgcggggag cgcgacggtc caattagtgt atgagtatca aaatataggg
      661 aaagtgtgat gattcgcgaa aatggcgtat ctttagccaa ggagtggata tggaatggat
      721 gggtggtcca tgagcctagg aatggcccgg gcgaagcatg gcccgatctc caccctgatc
      781 atgctgtcat gcggcttcgc cctggccttg gtcggcgtcg tcgtcctcgc cggctgggtc
      841 ctcggcctgc gcatcctcac gagcctccac cccgcctatc ttcccacagc gcccgatacc
      901 gccctcctct ttgtcctggt cggcgtgatc gtcgcgaccg agacgcgatg gaagtggggc
      961 gcgggtgcgc gaaaggccgc cgccctgctc cttgtcctcg tgggcctcta cggcgggttg
     1021 aaattcctcg aaggcgtggt cgggaccgac ctcagctttg cggggaggct attcccggtc
     1081 tccgagcgcc tgcgcggttt cccactccgg cggatgtccc cgatagccgg tttcctcttc
     1141 ctgcttggag ccgcggcgat gatcctgcgg gagcgcgcca tcgggcggcg ccggaccgcc
     1201 gacctggtcg gcctgcttgg atcgatcgtc gctctgctcg ccttcgtcgc cgtcctgggc
     1261 taccttttcg gcacgccctt gctgtatcgg ggcaccctga tacccttggc ggcgccgagc
     1321 tccttcgcct tcctgctctg ggggactgcg atcgtcgcct cggtggggag caaggcccgc
     1381 cccttccgac gctttgttgg ccgcgcgatg gacgcgagac tcatgaggag catcgttccc
     1441 ctcaccgtcg ccttcagcct tggcgggggc ctcctccagt ccctgctcgc gaagagcccg
     1501 aagtatgacg ccctcgaaag ctcgctcatt accctcacct tcctcgcggc ctcggctgtg
     1561 gtggtcatgc gggcctcgcg cagggtcttc cggagggccg aggcggccga gctcgagatg
     1621 cgccggctcg acgacgagaa gcgcgaatcg gagcggaagt accgccgcct ctacgagggc
     1681 atgagggacg gctatacgat cgccgacccc tcgggacgct acctcgagag caacgccgcc
     1741 ttccgggagc tcgtcggcta ctcggacgag gagctccgct cgctgagcgt cctcggcata
     1801 acccctgaga agtggcatcc cctccagagg cggatcatgg aggaggaggt cctggtgcgg
     1861 ggctattccg agctgttcga gaaggagtac aggcggaagg acggcagggt catcccggtg
     1921 gagctcagga tcttcctgat cagggacgag gccggcgggt ggctcggcac ctgctcggtg
     1981 gtcagggaca tatccgagcg gaagaaggcc gaggccgggc tgcgcgagag cgaggagcgc
     2041 ttcagggcca tcttcgagaa tgccggcatc ggcatggccc tcgtcgacaa ggagggccgc
     2101 atcacgaggg cgaacgcctc cctcgcggcc atgctcgatt accagctgtc cgagctggaa
     2161 ggccgcagcc tcatggagct cacccatccc gacgacaggg agagcgactg gaacctctac
     2221 ctcaacctga tcgccggcgc ccgggactcc tatcagatcg agaagcgcta tctgcgcaag
     2281 gacggcaggg tcatctgggg ccagctgacc gcctcgacct tctgcatccc ctcggagggc
     2341 tgctccatca tcggcatggt cgaggacgtc acaagccgcc gcgcccagga cgagctcata
     2401 aggaggtccc tgggcgagaa ggaggccctc ctcagggagc tccaccaccg cgtcaagaac
     2461 aacctccagc tcatcacgag cctgctctcg ctccaggggg cgagccttcc cgaggggccg
     2521 gccggggacg ccgtccgcgt cgctctccac gacgccaccg gcagggtcgg ggcgatggcg
     2581 gggatccacg agctggtcta cctctcgaag gacttctccg agatcgactt cggcgcctac
     2641 atggcctcga tagcccgcgg ccagctcctc gagctcggcg ggtcaaggcc catccggctc
     2701 gacctcgacc tcgaggagct caggatcagc ctcgacgacg cgatcccctg cggcctcctc
     2761 atcaacgagg ccctgagcaa cgtcgcgaag tacgcatacc cgccggactg gcccggccag
     2821 cgtcgggccg agctcagcct caggcgatcg gccgggggac ggcgccggat cgtcatccgc
     2881 gatcacgggg tggggatcca gcccgatcag cctgagtcta gggggctcgg cctccacatc
     2941 atgaggatcc tctcggacca gatcggaggc aggctggagg ttcgttcgga ggacggggtc
     3001 gaggtccagc tcgaattcta gaccgagcgc aggagctgga ggatgtctcc cccgtagcgc
     3061 tcgaccttgg tgggcccgag gccga
//
LOCUS       DASSQV010000028         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2246885, whole genome shotgun sequence.
ACCESSION   DASSQV010000028 DASSQV010000000
VERSION     DASSQV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2246885"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFL04_00480"
     CDS             <1..459
                     /locus_tag="VFL04_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838202.1"
                     /translation="RVVREIEGMLETAAALAMNRPTSLASLGGHHGATALSGTGATAM
                     AVDETGHPILPPILKTVAASGEGMAELAAAIRTHRADMAASGRLEARRLEGAVSEMRG
                     LIAYRLLDALEGPKGRELEARLARAVVDREYDAYEAADMLFAALKQGVEP"
     gene            456..863
                     /gene="mce"
                     /locus_tag="VFL04_00485"
     CDS             456..863
                     /gene="mce"
                     /locus_tag="VFL04_00485"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HET7838203.1"
                     /translation="MKILKIDHIGVAVENLEETLALYTGILGLELHGTETVEEQKVKT
                     AFLPVGDTEVELLASTDPEGPIGKFIAAKGQGVQHVAFRVADIEAALAELKAKGVRLI
                     DEKPRYGAGGARIAFLHPKATNGVLVELCERKA"
     gene            990..2534
                     /locus_tag="VFL04_00490"
     CDS             990..2534
                     /locus_tag="VFL04_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET7838204.1"
                     /translation="MDEKIRELNERRAKVLAGGGPKRVAAQHEKGKMTARERIESLLD
                     PGSFIEIDAFVEHRAVDLGMDEVDAPGEGVVIGHGTIDGRTIFVFAQDFTVIGGSLGE
                     MHALKICKAMDMAVKVGAPVIGINDSGGARIQEGVDALSGYGDIFFRNTRASGVVPQI
                     SVIMGPCAGGAVYSPALTDFTVMVDKTSNMFITGPQVIKAVTGEDVSAEALGGATVHS
                     TTSGCASLMFADEASTIAGIKKLLSYLPQNNIEDPRVLDSGDDPARTAPALKDVIPDA
                     PNKPYDCRDVIRNVFDSGDFFEIQPLYAQNIVTCFARLGGRSVGVIANQPKVLAGVLD
                     INASDKAARFIRFCDAFNIPLVTLADTAGYLPGVGQEHGGVIRHGAKLLYAYSEATVP
                     KMTVIVRKDYGGAYIAMCSRHLGADQVFAWPSAEIAVMGPDGAANIIFKREIEESSDP
                     AATRKDKIAEYKHNFANPYKAAIRGYVDDVIDPAVTRPRLIRALTMLLGKRESLPPRK
                     HGNMPV"
BASE COUNT          467 a          968 c          832 g          360 t
ORIGIN      
        1 cgcgtcgtgc gcgagatcga gggcatgctc gagaccgccg ccgccctcgc catgaaccgg
       61 cccacgagcc tcgcgagcct gggggggcac cacggcgcca cggccctgag cggcaccggg
      121 gccacggcca tggccgtgga cgagaccggc caccccattc tccccccgat cctcaagacg
      181 gtcgcggcga gcggggaggg catggccgag ctcgcggccg cgatcaggac ccaccgcgcc
      241 gacatggccg cgagcggcag gctcgaggcg cgcaggctcg agggggcggt ctccgagatg
      301 cgcggcctca tcgcctaccg cctcctcgac gccctcgagg gccccaaggg ccgagagctc
      361 gaggccaggc tcgcgcgggc cgtggtggac agggaatacg atgcctacga ggcggccgac
      421 atgctgttcg ccgcactaaa gcaaggagtt gaaccatgaa gatcctgaag atcgatcaca
      481 tcggggtggc tgtggagaac ctcgaggaga ccctcgccct gtacaccggc atcctcggcc
      541 tcgagctgca cggcaccgag accgtcgagg agcagaaggt aaagacggcc ttcctgccgg
      601 tgggcgacac cgaggtcgag ctcctcgcct ccaccgaccc cgagggcccc atcggcaagt
      661 tcatagcggc caagggccag ggggtccagc acgtggcctt cagggtggcc gacatcgagg
      721 ccgcgttggc cgagctcaag gccaagggcg tgaggctgat cgacgagaag ccccgctacg
      781 gcgccggcgg ggcgcggatc gccttcctcc accccaaggc cacgaatggg gtccttgtcg
      841 agctctgcga gcgcaaggca taatcggtca cgatcggccg gagacagaac gtcgatcgcg
      901 acctcgcggt cctgcggtgc cggaacttcg cgccgtggaa ccgcctggac caaagccaaa
      961 acttgaccct gaaaaggacg gttgaccgta tggatgagaa gatccgggaa ctgaacgagc
     1021 gccgcgcgaa ggtgctggcc ggaggcgggc ccaagcgcgt cgcggcccag cacgagaagg
     1081 gcaagatgac ggcccgcgag cgcatcgagt ccctcctcga tccgggcagc ttcatcgaga
     1141 tcgacgcctt cgtcgagcac cgcgccgtcg acctcggcat ggacgaggtc gatgcccccg
     1201 gcgagggtgt cgtcatcggc cacggcacga tcgacggccg caccatcttt gtcttcgccc
     1261 aggacttcac ggtcatcggc ggctccctcg gcgagatgca cgcgctcaag atctgcaagg
     1321 cgatggacat ggccgtcaag gtcggcgccc ccgtcatcgg catcaacgac tcgggcgggg
     1381 cccgcatcca ggagggcgtc gacgccctct cgggctacgg cgacattttc ttccgcaaca
     1441 ccagggcctc gggcgtcgtg ccccagatct ccgtgatcat gggcccctgc gccggcggcg
     1501 ccgtgtactc cccggccctc accgacttca ccgtcatggt cgacaagacc tcgaacatgt
     1561 tcatcaccgg gccccaggtc atcaaggctg tcaccggcga ggacgtgagc gccgaggccc
     1621 tcggcggggc caccgtccac tcgaccacga gcggctgcgc ctcgctcatg ttcgccgacg
     1681 aggcctcgac catcgcgggc atcaagaagc tcctctccta cctgccccag aacaacatcg
     1741 aggaccccag ggtcctggac tcgggcgacg acccggcccg cacggccccc gccctcaagg
     1801 acgtcatccc cgacgcgccg aacaagccct acgactgccg cgacgtgatc cgcaacgtct
     1861 tcgactcggg cgacttcttc gagatccagc ccctctacgc ccagaacatc gtgacctgct
     1921 tcgcccggct cggcggccgg agcgtgggcg tcatcgccaa ccagcccaag gtcctcgcgg
     1981 gcgtcctcga catcaacgcc tccgacaagg ccgcgcgctt catccgcttc tgcgacgcct
     2041 tcaacatccc cctcgtgacc ctcgccgaca ccgcgggcta tctgcctggc gtcggccagg
     2101 agcacggcgg ggtcatccga cacggagcaa agctgctgta cgcctactcc gaggccaccg
     2161 tgcccaagat gaccgtcatc gtccgcaagg actacggcgg ggcctacatc gccatgtgct
     2221 cgcgccacct cggcgccgac caggtcttcg cctggccctc ggccgagatc gcggtcatgg
     2281 gccccgacgg ggccgccaac atcatcttca agcgcgagat cgaggagtcc tccgaccccg
     2341 ccgccacgcg caaggacaag atcgccgagt acaagcacaa cttcgccaac ccctacaagg
     2401 cggcgatccg cggctatgtc gacgacgtca tcgacccggc ggtgaccagg ccccggctca
     2461 tcagggccct caccatgctc ctgggcaagc gcgagagcct gccgcccagg aagcacggca
     2521 acatgccggt ataggcggcc gcgcgatgga cagcacgacc ctctacctgg tcctcggcct
     2581 cgccgccctc ctcctcgtga tcctggtccg ctccctcgtg aagggca
//
LOCUS       DASSQV010000029         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_134112, whole genome shotgun sequence.
ACCESSION   DASSQV010000029 DASSQV010000000
VERSION     DASSQV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134112"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..538)
                     /locus_tag="VFL04_00495"
     CDS             complement(194..538)
                     /locus_tag="VFL04_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838205.1"
                     /translation="MAHDLVDSIMELSDRVRYVALLRGGELVTRARGGRANASSSESD
                     RYEELIVNPTLLTLLTQRGNIDCGGLRHVIIRYGSFFQLVLPIEGGHLSVCLEAEADP
                     VRLEAEIETLLG"
     gene            778..1191
                     /locus_tag="VFL04_00500"
     CDS             778..1191
                     /locus_tag="VFL04_00500"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7838206.1"
                     /translation="MDSILGEEYFTRELAEAILGEALAKRELRVARLRGEVVGFFRLA
                     LDGVFLVFAYVHLFAVKTGHRGQGLGTAMLNEAERLIRAERGYPDVKKSFLLVGKINR
                     RAKRFYESQGYRRIATLDDLFARGDTEYLMMKELA"
     gene            1223..2029
                     /locus_tag="VFL04_00505"
     CDS             1223..2029
                     /locus_tag="VFL04_00505"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HET7838207.1"
                     /translation="MAETEGRAQIVYFSHGGGPLPLLGDPGHFSMIEFMKALPARLRR
                     PTAVLVVSAHWEEGEPTLLSSDRPPMLYDYYGFPDKAYEIVYPAPGSPELARRIAGLL
                     GDSGLPARMDSDRGFDHGLFIPLAMMYPEAEIPCLQLSLVGGLSPGAHIALGRALRGL
                     LGEDILVIGSGFSFHNMRAFDWSSSNQPDPRNDAFQAWLVDTCTGARDEAERERLLSA
                     WEGAPSARYCHPREEHLLPLHVCAGMAGGPAELVFDDYILGKRAVAFLWA"
BASE COUNT          326 a          724 c          653 g          327 t
ORIGIN      
        1 tcagaagggg caagagggcg acgctgttga tcgcattgcc gccttcgtca ccgtagaagt
       61 cccgatccgc gctccgcacc aatgcctcaa gaccgctgcc catgctccac ctccagatcg
      121 ccagaagatg ctgaactgta gttcccccct cggcgccggt caagccgagg gagccattgc
      181 gttgtgcggc ggcctagccg agcagggtct cgatctcggc ctcgagccgg accgggtcgg
      241 cctcggcctc gaggcagacc gagaggtgtc cgccctcgat cggcaggacg agctggaaga
      301 aggagccgta gcggatgatg acgtggcgca ggcccccgca gtcgatgttg cccctttggg
      361 tgagcagggt gaggagggtg gggttgacga tgagctcctc gtacctgtcc gactcggagg
      421 agctcgcgtt cgcccggccc cccctcgccc tcgtgacgag ctcgcccccg cggaggaggg
      481 cgacgtatct gacccggtcc gagagctcca tgatcgaatc gacgagatcg tgcgccacgg
      541 gccgcctcct cccgcaagcc tagctcctcc cgggcgggcg cgcaatcgag gcccgcggta
      601 gggcggcatt ggccgcggcg cccggcggaa ccatggatcg cttccgaatt gccagcgtca
      661 ccatctcgat gtaagcttga gccaatgaaa aaggaagccg ccaaaccgag cgctgccgcc
      721 aagcccggct aacgcctcgc caccgagggg gacctgcccg cctgtctcga catcctcatg
      781 gactcgatcc tcggcgagga atacttcacc cgggagctcg ccgaggccat cctgggcgag
      841 gccctcgcga aaagggagct ccgcgtcgcg aggctgaggg gcgaggtcgt cggcttcttc
      901 cgcctcgccc tcgacggggt cttcctggtc ttcgcctacg tccatctttt cgcggtcaag
      961 acgggccacc gcgggcaggg gctcgggacg gcgatgctca atgaggccga gcgcctcatc
     1021 cgcgcggaac gcggctatcc cgacgtcaag aagtccttcc tcctcgttgg caagataaac
     1081 cggagggcca agcgcttcta cgaaagccag ggctaccggc gcatcgccac cctggacgac
     1141 cttttcgccc ggggcgacac ggagtacctc atgatgaagg agctcgcata gcgcaggcta
     1201 gggaagaaga caggaggctg atatggcaga gaccgaagga cgggcccaga tcgtctactt
     1261 ctcccacggg ggcggccccc tgcccctgct cggggatccg ggccacttct ccatgatcga
     1321 gttcatgaag gccctgcccg cgaggctcag acggcccacg gccgtcctcg tcgtcagcgc
     1381 ccactgggag gagggggagc cgaccctcct ctcctccgat cggccgccga tgctctacga
     1441 ctactacggc tttcccgaca aggcctacga gatcgtctat cccgcgcccg ggtcccccga
     1501 gctggcccgg cgcatcgccg ggctcctcgg cgactctggc ctcccggccc ggatggacag
     1561 cgaccggggt ttcgaccacg ggctcttcat accgctcgcg atgatgtacc ccgaggccga
     1621 gatcccctgc ctccagctat ccctcgtcgg aggcctctcc cccggagccc acatcgcgct
     1681 ggggcgggcc ctccgcggcc tcctgggtga ggacatcctg gtgatcggct cgggtttctc
     1741 cttccacaac atgcgcgcct tcgactggag ctcctcgaac cagcccgacc cgcgcaacga
     1801 cgctttccag gcctggctcg tcgacacctg cacgggtgct cgcgacgagg ccgagcgaga
     1861 gcggctgctc tcggcctggg agggggcgcc ctcggcccgc tactgccatc cgcgcgagga
     1921 gcacctcctt cccctccacg tctgcgcggg catggcgggc gggccggccg agctcgtctt
     1981 tgacgactac atcctgggga agcgcgcggt cgccttcctc tgggcctaaa
//
LOCUS       DASSQV010000030         4981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472 k141_9650,
            whole genome shotgun sequence.
ACCESSION   DASSQV010000030 DASSQV010000000
VERSION     DASSQV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9650"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFL04_00510"
     CDS             <1..437
                     /locus_tag="VFL04_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838208.1"
                     /translation="YANVKSIYPNDEGLTFQTDGKKLFILVEPPNYPNKGMEPFVRSS
                     AEKIPLRFSELDLYTSKNQTKIYWAKEAVISYGSFTILRPAGINFSFCFYFLPDMYQS
                     MQLFFEKTLSKEVGLPLSDSKKVAAAVAAKIKTAMVLDYSSE"
     gene            complement(492..2132)
                     /locus_tag="VFL04_00515"
     CDS             complement(492..2132)
                     /locus_tag="VFL04_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838209.1"
                     /translation="MTKYAHRIALMTFLVISIASCSLLTQTKTTELKSPAFTSRFLVG
                     TGAPNSSTAGSVGDLYLDKSTVELWYLDNAGWTKVATLQGPAGPVGATGPAGPAGATG
                     ATGATGATGATGATGATGATGATGAPGANGSPGAPGSPGANGSTWYTGSGAPSNGLGV
                     DGDLYLDAASAAVYRKSAGAWGFLVSLQGPAGTSGTNGTNGTNGTNGTNGTNGTNGVD
                     GSTWLTGSGAPGGGLGKNGDLYLDSLNALVYVKAVGVWTELFGFPMAQAQIVYTSFTT
                     QFSQNALGYGNGTFVMTGFSGGGVETSTDGSHWSSLISAGSNTLNAVAYGNGTFVAVG
                     LAGAILYSSDAQTWTAATNPAGSEVYLNAVTYADGMFMAVGGLGLILTSPDGVTWTQQ
                     TSNVSTNLLSIAYGNGSFVVVGANFESGNPTILTSGDGVTWTTQDSSALAYDTLQSVA
                     FGNGAFVLVGSSGLLASSSDGITWSVQTLFAHGFHDVVFSATGNGSFVVLGDSSVLSS
                     PYGSAWSSQYWDYISPAVFACAAAGGSVVVVVAKVTPS"
     gene            complement(2351..>4981)
                     /locus_tag="VFL04_00520"
     CDS             complement(2351..>4981)
                     /locus_tag="VFL04_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HET7838210.1"
                     /translation="NIHFFSQKETVKKGVGAEKLGMRGRQANEFAELSLPILPGFIID
                     CDAAPRLAGEDVLGLIRPHLKKVEEIVGKSFGDSKNPLLFKIVVSPNLAISNYPTLHD
                     FGLARDTVEGFASWVGANFAAHEVLFLVRGMLAVEDRLAELEGRDKDRASLAELAQRV
                     GKGLASDKPGKSAQALMDEYAPILPEGFFDGAEKQLEIALKRVSRLIKLDEQDADDTA
                     LLVQPMVYGNYGKDSASGAFFTRDIVLGEPVLQGEYYQERFNEIGATGKDITKIDPSY
                     LKELEKIARKIEDKHLEIRQIRFTIENKKLWLIEQRQVVAKSTQADLRLALDLNGRKV
                     VNDEYVVMSIAPGRLNEILHPIVDLGSVKKLEGVSGGIAGAPGAAIGRVFFSTESLLE
                     AHREAQQKSQDKRLILCMNSTFAEDVKAIEVATGVLSAEGGFSAHASVVARQYGKVSM
                     VKPEMRIRGKKAVIGELAINEGDYITINVPYYGEPQIWLGKADLIEPNPKDSGLLDFI
                     DLAKKQMTHFHVRANSDSPRDASMALLFGAEGIGLCRTEHMFFNEKRINVIREMILSD
                     TQEERIKALEKLKPMQREDFYGLFKVMAGKEVTIRLLDAPLHEFLPHNEPEMDALVAY
                     LGARKGATKLTKAEIRARCDSLSEFNPMLGHRGCRIAVSYPEIYDMQIRAIFEAVYRL
                     QKEKIDVRPEVMIPVVMNSSELKLVNFGKKIEGKQYRGLVEIEEEVRKELGAKPVPFK
                     IGTMVELPAAALSAGEIARYSEFFSFGTNDLTQTTLGISRDDFNSFMPDYSLYDLIEG
                     NPFSVLAPPVKELIGMAVHRGKLSRPTLVTGLCGEHGANPDNIRFCMDAGLDYVSCSP
                     YQVPLSITAVAQAEIERRKA"
BASE COUNT          803 a         1567 c         1669 g          942 t
ORIGIN      
        1 cctacgcgaa cgtgaaatcg atctacccaa acgatgaggg cctcaccttc cagaccgacg
       61 ggaagaagct cttcatcctc gtcgagccac ccaactaccc gaacaagggc atggagccct
      121 tcgtgcgctc atcggccgag aagatacccc tcaggttcag cgagctcgac ctctacacca
      181 gcaagaacca gacgaagatc tactgggcca aggaggcggt gatctcctac ggctccttca
      241 ccatcctcag gccggcgggg atcaacttct ccttctgctt ctacttcctc cccgacatgt
      301 accagtcaat gcagctcttc ttcgagaaga ccctcagcaa ggaagtgggc ctccccctgt
      361 ccgactccaa gaaggtcgcc gccgccgtgg ccgccaagat caagaccgcc atggtcctcg
      421 actacagctc ggagtagcga tcgggatccc cccgggaccg ggcccagggc ccggatcccg
      481 ggagccgact cctaggacgg cgtgaccttg gccacgacca cgaccaccga gccgccggcc
      541 gcggcgcagg cgaacacggc cgggctgatg tagtcccagt actgggagct ccatgcgctt
      601 ccgtaggggc tcgaaagcac gctgctgtcc ccgaggacca cgaatgagcc gttgcccgtg
      661 gcgctaaaga ccacgtcgtg gaagccgtgg gcgaagaggg tctggaccga ccaggtgatc
      721 ccgtcgctgc tcgaggccag gaggccgctg ctgccgacga gcacgaaggc tccgttcccg
      781 aaagccacgc tctgaagcgt gtcgtaggcg agggccgagc tgtcctgggt cgtccaggtc
      841 accccatcgc cgctcgtcag gattgtcgga ttcccggact cgaagttcgc gcccaccacc
      901 acgaagctgc cgttcccgta ggcgatggaa aggaggttgg tgctgacgtt cgaggtctgc
      961 tgggtccagg tcaccccatc ggggctcgtg aggatgaggc ccaggccgcc cacggccatg
     1021 aacatgccgt cggcgtaggt gacggcgttg aggtagacct cgctgcccgc cgggttcgtg
     1081 gccgccgtcc aggtctgggc gtcactgctg tagagaatgg cgcccgcgag ccctacagcc
     1141 acgaaggttc cgttgccgta ggcgacggca ttgagggtgt tcgaaccggc gctgatcagg
     1201 cttgaccagt gcgagccatc ggtgctggtc tccactcccc ctccggagaa gcccgtcatc
     1261 acgaaggtgc cgttgccgta gccgagggcg ttctggctga actgcgtcgt gaaggaggtg
     1321 tagacgatct gggcctgggc catgggaaag ccgaagagct cggtccagac gcccaccgcc
     1381 ttgacgtaga ccagggcgtt caggctgtcc agatataggt cgccgttctt gccgaggccg
     1441 ccgccgggcg cgccgctgcc ggtgagccag gtgctgccat cgacgccgtt tgtgccgttg
     1501 gtcccgttgg tcccgttcgt gccattggtc ccgttcgttc cattcgtgcc gcttgtcccg
     1561 gccggcccct gcaacgagac gaggaagccc caagccccgg cggatttccg gtatactgcc
     1621 gcgcttgccg catcgaggta gaggtcgccg tcgacgccga gaccgttgct cggcgccccg
     1681 ctgccggtat accaggtgct gccgttcgcc ccgggactgc ctggggcgcc ggggcttccg
     1741 ttcgcccctg gggcgcccgt cgctccggtt gcgcctgtcg cgcccgttgc tccggtcgcg
     1801 cctgtcgcgc cggtggctcc ggtcgctccg gtcgctcctg cgggaccggc ggggcccgtt
     1861 gctccgacag gccctgcggg tccctggagg gtggccacct tcgtccaacc cgcgttgtcc
     1921 aggtaccaca gctcgacggt gctcttgtca aggtagaggt ccccgacgga gccggcggtg
     1981 ctcgagttgg gcgcaccagt cccaacgagg aagcgtgagg tgaaggccgg gctcttgagc
     2041 tcggtcgtct tggtctgggt cagaagggaa catgaggcga tcgaaatgac gagaaaggtc
     2101 atgagggcga tgcgatgggc gtactttgtc atcatgtgct ccttgggcga aaaagagctt
     2161 gttcctgaat atactgaggc tcgccctata tcttagcgat ttagtcatct tttctagcgg
     2221 acagcgcgca tgacccgccc gagaaatgcc gcagccagga ctcgggcgca ggcgccttga
     2281 gtcccgggcc caaaagcgaa aaacccccgg cctggctggc cgggggctgc ggtttcgggc
     2341 ttgcctggtc ctaggccttt cttcgctcga tctccgcctg ggccaccgcc gtgatggaca
     2401 ggggcacctg gtagggcgag catgagacat agtcgaggcc cgcgtccatg cagaagcgga
     2461 tgttgtcggg gttggcaccg tgctcgccgc agagccccgt caccaaggtg ggcctcgaga
     2521 gcttgccgcg gtggacggcc atgccgatga gctccttgac cgggggggcg agcaccgaga
     2581 aggggttgcc ctcaatgagg tcgtagagcg agtagtcggg catgaaggag ttgaagtcgt
     2641 cccggctgat gcccaaggtg gtctgggtca ggtcattcgt cccgaaggag aagaactcgc
     2701 tgtagcgggc gatctccccc gcgctcaggg ccgcggccgg gagctcgacc atcgtgccga
     2761 tcttgaaggg gacgggtttg gcgccgagct ccttgcgcac ctcctcctcg atctccacga
     2821 ggccccggta ctgcttcccc tcgatcttct tcccgaagtt gaccagcttg agctcggacg
     2881 agttcatgac cacggggatc atgacctcgg gcctgacgtc gatcttctcc ttctggagcc
     2941 gatagacggc ctcgaagatg gccctgatct gcatgtcgta gatctcgggg tagctgacgg
     3001 cgatgcggca gccgcggtgg cccagcatgg ggttgaactc ggagagggag tcgcagcggg
     3061 cgcgtatctc ggccttggtg agcttggtgg cgcccttcct cgctccgaga taggccacga
     3121 gggcgtccat ctcgggctca ttgtgcggca ggaactcgtg caggggcgca tccaggaggc
     3181 ggatggtcac ctccttgccg gccatgacct tgaagaggcc gtagaagtcc tccctctgca
     3241 tcggcttgag cttctccagg gccttgatcc tctcctcctg ggtgtcggag aggatcatct
     3301 ccctgatgac gttgatgcgc ttctcgttga aaaacatgtg ctcggtgcgg cagaggccga
     3361 tgccctcggc cccgaagagg agggccatgc tcgcatcgcg cgggctgtcg gagttcgcgc
     3421 gcacgtggaa atgggtcatc tgcttcttag cgagatcgat gaaatcgagg aggcccgagt
     3481 ccttggggtt gggctcgatg aggtcggcct tgcccagcca gatctggggc tcgccgtagt
     3541 agggcacatt gatggtgatg tagtcgccct cgttgatggc caactcgccg atcaccgcct
     3601 tcttgccgcg gatgcgcatc tcgggcttga ccatggagac cttgccgtac tggcgcgcga
     3661 cgaccgaggc gtgggccgag aagccgccct cggccgagag gacgccggtg gccacctcga
     3721 tggccttgac gtcctcggcg aaggtcgagt tcatgcagag gataagccgc ttgtcctggc
     3781 tcttctgctg ggcctcgcgg tgggcctcga gcagggactc ggtgctgaag aagaccctgc
     3841 cgatggccgc gccgggggct ccggcgatgc cgccagacac gccctcgagc ttcttcaccg
     3901 agccgaggtc gacgatgggg tggaggatct cgttgagccg gccgggggcg atcgacatga
     3961 cgacgtactc gtcgttcacg accttgcggc cgttgaggtc gagggcgagc ctcaggtcgg
     4021 cctgggtgct cttggccacg acctggcgct gctcgatgag ccagagcttc ttgttctcga
     4081 tggtgaatcg gatctgcctg atctcgaggt gcttgtcctc gatcttgcgc gcgatcttct
     4141 cgagctcctt gaggtaggag gggtcgatct tggtgatgtc cttgccggtg gcccctatct
     4201 cgttgaagcg ctcctggtag tactcccctt ggagcacggg ctcgcccagc acgatgtcgc
     4261 gggtgaagaa ggcacccgag gccgagtcct tgccgtagtt gccgtagacc atgggctgga
     4321 cgagaagggc ggtgtcgtcg gcgtcctgct cgtcgagctt tatgagccgc gacacccgct
     4381 tgagggcgat ctcgagctgt ttctctgccc cgtcgaagaa tccctcgggg aggatggggg
     4441 cgtactcgtc catcagggcc tgggcgctct tgcccggctt gtccgaggcg aggcccttgc
     4501 cgacgcgctg ggccagctca gcgagcgagg ccctgtcctt gtcccggccc tccagctcgg
     4561 cgagcctgtc ctcgaccgcc agcatgcccc gcacgaggaa gaggacctcg tgggcggcga
     4621 agttggcccc gacccaggag gcgaagccct cgaccgtgtc gcgggcgagg ccgaagtcat
     4681 ggagggtcgg gtagttggag attgcgaggt tcggtgagac gacgatcttg aaaagcaggg
     4741 ggttcttgga gtcgccgaag ctcttcccga cgatctcctc aaccttcttg aggtggggcc
     4801 ttatgaggcc gaggacgtcc tccccggcca gcctgggggc cgcgtcgcaa tcgatgatga
     4861 aacccggaag gatggggagg gacagctcgg cgaactcgtt ggcctggcgg ccccgcatgc
     4921 ccagcttctc agccccgacg cccttcttga ccgtctcctt ctggctgaag aaatgaatgt
     4981 t
//
LOCUS       DASSQV010000031         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1750494, whole genome shotgun sequence.
ACCESSION   DASSQV010000031 DASSQV010000000
VERSION     DASSQV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750494"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VFL04_00525"
     CDS             <1..749
                     /locus_tag="VFL04_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838211.1"
                     /translation="PAGSLGGPTVGLGAAAADAPGAGLGARPGYGAGPVPAYRKMLPF
                     SDNPPEADRMAALAGAAAILGGALTTQAVLFWWSLGPELRYERYALETLVSAFGLLPP
                     ALTLGFFGLLPLAAGAAGLILPLGRELGRLRENARRRALNARILAGRRVLEAAGELEL
                     ACLDTRRSRAAARRSLDSLAAEFGASVSVPEPPSDAASGLPGLREPGCRLSYSFPRLA
                     ELLAELERLRGPTPPAGEEGGGGIVFDSGD"
     gene            878..1861
                     /locus_tag="VFL04_00530"
     CDS             878..1861
                     /locus_tag="VFL04_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7838212.1"
                     /translation="MKKAAWILAFVFGLAFLGATLMNADLIAGSRKALARGVLRDKSS
                     IPAARYQLVVVLPDTDDSFFRGILDGIREAAPSFESAVQVFRYPQASVSEAARYYEIA
                     LRAKVDGLIMYTPRNDLDAGRGERARRNGVIFVPVGTDAPPGSRPGFIGSGSLLQGFE
                     GGTLICAQLGSSSRIGVILPSSGTGDPGEEPLYRGVSAAIKAFPGATIAAVARAQPGV
                     LSGEEAASSLLRKDPSINALFCSSSLDTTGAAQVVVDLNRVGRIVIVGADETPEIRRY
                     IDKGVVAASIVRDSRRIGLEAVRLFSRLKAGALATETVEVGFSVKLSKGAE"
     gene            1858..>3248
                     /locus_tag="VFL04_00535"
     CDS             1858..>3248
                     /locus_tag="VFL04_00535"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7838213.1"
                     /translation="MRLPRTMRGKIIANFAIILLIIASATLYTGLASSELARSVEILF
                     RNNLVMENISRALDLTESSLTGYLTSKSSDALKDYIMYSTRLADEARNLNREIREDES
                     LLLQRDLAALIDSYLEDTEATVAAKRGRDVNAYTTRFEASDRAADLSRFLIARIEGIF
                     ISDSLAAFSAYNSRVSGVIASNAALVLAATLMGLVLVVRFSFRLTEPLSRLAEAARAV
                     ARGEYDSELPAPETGDEIGTTAAAFARMQESVRRAFEEVREKSEVEKRLMEERMRVLD
                     MSHKLKDAELLALQTQINPHFLFNTLSAGMQLAMTEGADQTSEFLDKLAMFIRYALKP
                     PSRSVRVSDEIECVERYIWLLRLRFRERFAFEVSADEEVLSVETPALVLQPLVENAVG
                     HGLRDREQGGTVRVRARLIAGEAVLEVEDTGEGMSAEEVERALREEEEGRDGREAARE
                     HGIGLRNVIRRVSL"
BASE COUNT          455 a         1203 c         1114 g          476 t
ORIGIN      
        1 ccccggcagg ctcccttggg ggcccgacgg tcggcttggg ggccgcagct gcggacgccc
       61 ctggggccgg gctcggggcc aggcccgggt acggggccgg cccggtcccg gcctaccgaa
      121 agatgctccc cttctcggac aacccccccg aggccgaccg gatggccgcc ctggccggcg
      181 cggcggcgat cctcggcggg gccctgacca cccaggccgt cctcttctgg tggagcctcg
      241 ggcccgagct ccgctacgag cgctacgccc tcgagaccct ggtctcggcc ttcggccttc
      301 tgcctcccgc cctcaccctc gggttcttcg gcctcctgcc cctggccgcc ggggcggcgg
      361 gcctgatcct gcccctgggc cgggagctcg gccggctcag ggagaacgcg aggcggaggg
      421 cgctcaacgc gcgcatcctc gccgggaggc gggtcctcga ggccgcgggg gagctcgagc
      481 tggcctgtct cgacacccgc aggtcgaggg ccgcggcgag gcgctccctc gattccctcg
      541 ccgccgagtt cggggcgagc gtctcggtcc cggagccgcc gtccgacgcg gcttcgggtt
      601 tgcctggcct tcgcgagccg ggctgccgcc tctcctacag cttcccccgc ctggccgagc
      661 tcctcgccga gctggaaagg ctccggggac cgacgccgcc ggccggggag gaggggggag
      721 ggggcatcgt cttcgactcg ggcgactgag gcccgccctt ccggcgcggg tgggcgccct
      781 tccggtgcgg gggggcgccc ttccggtgcg ggggggcgcc cctccgggcc ggggcgcgct
      841 gccgcttctg gccagacatg gtatactctg ccgagccatg aaaaaggcgg cctggatcct
      901 cgccttcgtc ttcggtctcg ccttcctcgg cgcgaccctc atgaacgccg acctcatcgc
      961 gggcagccgc aaggccctcg ccagaggggt gctgcgcgac aagtcctcga tacccgcggc
     1021 gcggtaccag ctcgtcgtcg tccttcccga caccgacgac tccttcttcc gaggcatcct
     1081 cgacgggatc cgggaggccg ccccctcctt cgagtcggcc gtccaggtct tccgctaccc
     1141 ccaggcctcg gtctccgagg ccgcgcggta ctacgagata gccctcaggg ccaaggtgga
     1201 cgggctcatc atgtacacgc cgaggaacga cctggacgcc ggccgcggcg agcgggcgag
     1261 gaggaacggg gtcatcttcg tgcccgtcgg gaccgacgcc ccgcccggct cgaggccggg
     1321 cttcataggc tcgggctccc tcctccaggg cttcgagggc ggcacgctga tctgcgccca
     1381 gctcggctcc tcgagccgga tcggcgtcat cctgccctcc tcgggcacag gcgatccggg
     1441 agaggagccc ctgtaccgcg gggtctcggc ggccatcaag gccttccccg gcgccaccat
     1501 cgccgccgtg gccagggccc agccgggggt cctctcgggc gaggaggcgg cgtcctcgct
     1561 cctccgcaag gacccgagca tcaacgccct cttctgctcc agttccctgg acaccacggg
     1621 ggccgcccag gtcgtcgtcg acctgaaccg ggtgggacgg atcgtcatcg tcggggccga
     1681 cgagaccccc gagatcaggc gctacatcga caagggcgtc gtcgcggcga gcatcgtgcg
     1741 cgattcgagg cggatcgggc tcgaggcggt caggctcttc tcaaggctca aggccggggc
     1801 cctggccacg gagaccgtcg aggtcggctt cagcgtgaag ctttccaagg gggccgaatg
     1861 aggctcccga ggacgatgcg cggcaagatc atcgcgaact tcgccatcat cctcctcatc
     1921 atcgcctcgg cgaccctcta cactggcctc gcctcctcgg agctcgcgcg gagcgtggag
     1981 atcctcttcc gcaacaacct cgtcatggag aacatcagca gggccctcga cctcaccgag
     2041 tcgagcctca ccggctacct cacgagcaag agctcggatg cgctcaagga ctacatcatg
     2101 tactccaccc ggctcgccga cgaggccagg aacctgaacc gggagatcag ggaggacgag
     2161 tccctgctcc tccagaggga cctcgccgcc ctcatcgaca gctatctcga ggacaccgag
     2221 gccacggtcg ccgccaagcg gggccgcgac gtcaacgcct acaccacccg cttcgaggcc
     2281 tcggaccgcg ccgccgacct ctcgcgtttc ctcatcgcca ggatcgaggg gatcttcatc
     2341 tccgactcgc tcgcggcctt ctccgcctac aactcccgcg tctccggcgt catcgcctcc
     2401 aacgcggccc tggtcctcgc cgcgaccctc atgggcctcg tcctcgtggt gcgcttctcc
     2461 ttcaggctca ccgagcccct gtcgcggctc gccgaggcgg cgcgggcggt ggcccgcggg
     2521 gagtacgaca gcgagctgcc ggcccccgag accggcgacg agatcggcac gacggccgcg
     2581 gccttcgccc ggatgcagga gagcgtgcgc agggccttcg aggaggtcag ggagaagtcc
     2641 gaggtcgaga agcgcctcat ggaggagagg atgcgcgtcc tcgacatgag ccacaagctc
     2701 aaggacgccg agctcctcgc cctccagacc cagatcaacc cccacttcct cttcaacacc
     2761 ctctcggcgg gcatgcagct cgcgatgacc gagggggccg accagaccag cgagttcctc
     2821 gacaagctcg ccatgttcat acgctacgcg ctgaagccgc cctcgcgctc ggtgcgcgtc
     2881 tccgacgaga tcgaatgcgt tgagcgctac atctggctcc tccgcctgcg cttccgcgag
     2941 cgcttcgcct tcgaggtatc ggcggacgag gaggtgctct cggtcgagac cccggccctc
     3001 gtcctccagc ccctcgtgga gaacgcggtg ggccacggcc ttcgcgacag ggagcagggc
     3061 ggcacggtga gggtcagggc caggctcatc gccggcgagg ccgtcctcga ggtcgaggac
     3121 acgggcgagg ggatgagcgc ggaggaggtg gagagggccc tgcgcgagga ggaggagggc
     3181 cgcgacggcc gcgaggcggc gcgcgagcac ggcataggcc tgcggaacgt gatcaggagg
     3241 gtgtcgct
//
LOCUS       DASSQV010000032         4424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2248154, whole genome shotgun sequence.
ACCESSION   DASSQV010000032 DASSQV010000000
VERSION     DASSQV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2248154"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..305
                     /locus_tag="VFL04_00540"
     CDS             36..305
                     /locus_tag="VFL04_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET7838214.1"
                     /translation="MGKKLYVGNLSYNTYEDSLRNFFQSFGMVVSAKIITDRDSGSSK
                     GFGFVEMGSEDEARNAIQGANGADLDGRQIRVNEAMDKPRRDDRY"
     gene            complement(372..632)
                     /locus_tag="VFL04_00545"
     CDS             complement(372..632)
                     /locus_tag="VFL04_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838215.1"
                     /translation="MAIDPADGPRTELSSRAEATLGSILDDLGFDCGSMRDELLRFIE
                     AELAVEPCANRKEVLLELRAYALAKTNKELIFHNIKRILDNY"
     gene            complement(853..925)
                     /locus_tag="VFL04_00550"
     tRNA            complement(853..925)
                     /locus_tag="VFL04_00550"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(890..892),aa:Lys,seq:ttt)
     gene            complement(973..2127)
                     /locus_tag="VFL04_00555"
     CDS             complement(973..2127)
                     /locus_tag="VFL04_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838216.1"
                     /translation="MRLEQHSIALTILLLASALAFAETPEIPPFGPLSGRALAMGGRH
                     FALADDIDTILANPAGLASAPRQLSVSRLQTRLTGPVFDIANAVVGGSDPTTALTDII
                     KNNDYKLNSGLSMAGPLSFGYVDGGIGFGLYNETKASLNAATISVVDLSVAEDVLLAG
                     GYAFGFDLGRGNRLDIGVIGKGFVRGEVAVSKSIFEVQSLLSNPSSLMSSQPFAETTG
                     IGLDLGLRWSLDKTLAAGIACRDAFSPALETKYPGVDGFLNSPAVNTSRYVIIAPNLG
                     LGLMWKPPLGRLGQVIDSLLLVADYSDILDLFSPIPRNAILNVSLGVETKVLDILALR
                     AGIRDALPSAGVGLDLGVFSVDLTAYGEELGNEPGMRPIYNLLLNFDFRY"
     gene            complement(2149..2883)
                     /locus_tag="VFL04_00560"
     CDS             complement(2149..2883)
                     /locus_tag="VFL04_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838217.1"
                     /translation="MRISQALRAGIAAPSIVLVLTLVGCQQLFTTSLGSSLARSKPPV
                     PSSLTATQAATYAADVTANRDVPLATALMPAMADLVAANPANTDILSDAVATAGIATG
                     VEEAFMKALNVVDISTLTSSSPNLDATQTSELASLLGSVAVSPDTTSVFTALAAANPA
                     DMAASGANATQYVVASIALVVADAQSQGADVAALLQQSPPFSSGYTPSLATDNLVTSL
                     MTNASTQWPGDQFISGMQGMFGNALP"
     gene            complement(2980..4119)
                     /locus_tag="VFL04_00565"
     CDS             complement(2980..4119)
                     /locus_tag="VFL04_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838218.1"
                     /translation="MKHPIVRLFLCLIAAAPALAQGFTYIAPKDAKAMGLGGSFSALS
                     SGFSSFYGNPAGFASPSADFTVLDLAAWAYFKPTSANTSRLLRIIQGKNTESQTVSSV
                     NDMLIENGFGGGAMAGLGYTGKGVGLGFFAVTDNYASGDTIMGVRMTSSTSVNAVIGL
                     GTPIRIGDLKLAIGGDARPFYRIDSSPGGWSLLDLITGSGNVMDNPVTAGFGLAMDFG
                     ASLDLEPFSFGLSIRDISPSFMMDSTTLGSVGSSVSGGGLALPESSATRAQPLPIVSV
                     GASWKPRLVRHFIDPSLYVEVQDPVGIYKRQESAWSLLHVGAELRFLNFITARAGINK
                     GWLSAGLGLDLYVLRLDGAVFTEELGLHPGDRPRTGVVLQAAIRF"
     gene            complement(4131..>4424)
                     /locus_tag="VFL04_00570"
     CDS             complement(4131..>4424)
                     /locus_tag="VFL04_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838219.1"
                     /translation="LCAADAAYDTMGTSIGASGSQGAMPAADSVQAAIVAAFVAAVPG
                     GGADSPQDKADALWDAMTLATPLSSYTPPSTSSGTSLGAIVAAAGLDLSKYGI"
BASE COUNT          766 a         1342 c         1593 g          723 t
ORIGIN      
        1 agccggggcg aggcctcgga caaaggaaca tcagaatggg caagaaactc tacgtcggca
       61 acctcagcta caacacctac gaggacagcc tccgcaactt cttccagtcc ttcggcatgg
      121 tggtctccgc caagatcatc accgaccggg actccggcag ctccaagggc ttcggcttcg
      181 tggagatggg ctccgaggat gaggcccgca acgcgatcca gggcgccaat ggcgccgacc
      241 tcgacggccg ccagatcagg gtcaacgagg cgatggacaa gccccgccgc gacgaccgct
      301 actaatcggc gcccgaaccc acatcgccga gggagccgcc cgcgagggcg gctcctctct
      361 ttggatcccc atcagtagtt gtccagaatc ctctttatat tgtggaagat cagctccttg
      421 ttggtcttcg cgagcgcgta tgcccggagc tcgaggagga cctccttcct gttcgcgcat
      481 ggctcgacgg cgagctcggc ctcgatgaac ctcaggagct cgtcccgcat gctgccgcag
      541 tcgaagccca ggtcgtccag gatggagccg agggtcgcct cggctcgaga gctcagttcg
      601 gtccgcggcc cgtcggcagg atcgatagcc atgcagtcct ccttggggaa agggaaagcg
      661 gctgatataa ttatataaca agccgggccg agaaatccat gtcagggccc cggaaaaaac
      721 cggagccccg accgcggagc cttggggagt gaagcgtttg attgagtggg gggtttgaag
      781 ggaaccgggg ccgtggatcc ggcggccccg ctggggcgcc ggtcccggcc tcgtcatgtc
      841 gatcgtcgcg gatgagccgc acgtgattcg aacacgtgac ccacgcctta aaagggcgtt
      901 gctctaccta ctgagctagc ggcccgcggg gctacagtag ctggtgctca ggctccatgt
      961 caatcgaccc ctttagtagc ggaagtcgaa gttcaggagg aggttgtaga tgggccgcat
     1021 gccaggctcg ttcccgagct cctcgccgta ggcggtcaga tccacggaga agaccccgag
     1081 gtcgaggccg accccggcgc tcgggagggc gtccctgatc cccgccctga gggccagtat
     1141 gtcgaggacc ttcgtctcga cgccgaggct cacattcagg atggcgttgc gggggatcgg
     1201 gctgaagagg tcgaggatgt ccgagtagtc ggcgaccagg agaagggaat cgataacctg
     1261 cccgagcctc cccaggggag gcttccacat aagaccaagg cccaggttgg gggcgatgat
     1321 gacatagcgg ctcgtgttca cagccggaga gttgaggaag ccgtcgaccc cggggtattt
     1381 cgtctccagg gcggggctga aggcgtcgcg gcaggcgatc cctgccgcca gggtcttgtc
     1441 gaggctccac ctcaggccca ggtcaagccc gatcccggtg gtctcggcga agggctgtga
     1501 gctcatgagg gagctgggat tcgagaggag gctctggacc tcgaagatgc ttttgctgac
     1561 cgcgacctcg cccctgacga agcccttccc gatgacgccg atgtcgagcc tgttgccccg
     1621 gccgaggtcg aagccgaagg cgtagccccc ggctagcagg acgtcctcgg ccacgctcag
     1681 atcgacgacg ctgatggtcg ccgcgttgag cgacgccttc gtctcgttgt agaggccgaa
     1741 gcctatcccc ccgtcgacat agccgaagga gaggggcccg gccatggaga gccccgagtt
     1801 cagcttgtag tcgttgttct tgatgatgtc ggtgagggct gtggtcgggt cgctgccccc
     1861 caccaccgcg ttggcaatgt cgaagacggg acccgtgagc ctggtctgta gccttgagac
     1921 ggacagctgt ctcggggccg aggccagacc ggccggattg gccaggatcg tgtcgatgtc
     1981 gtcggcgagg gcgaagtgcc ttccgcccat ggcgagggcc cggcccgaga gggggccgaa
     2041 gggcgggatc tccggcgtct ccgcgaaggc cagggcactg gcgaggagca ggatggtgag
     2101 ggctatgctg tgctgctcga ggcgcatcct tgagactcct tgccgctcct agggtagggc
     2161 gttgccgaac atgccctgca tgccggagat gaactggtcg cccggccact gggtggaggc
     2221 gttcgtcatg aggctcgtca ccaggttgtc ggtggcgagc gagggcgtat agcccgagga
     2281 gaagggcggg ctctgctgga gcagggccgc cacgtcggcg ccctggctct gggcgtcggc
     2341 caccacgagg gctatcgagg cgacgacata ctgggtcgcg ttggccccgc tggccgccat
     2401 gtccgcgggg ttcgccgccg ccagagctgt gaacacgctc gtggtatcgg ggctcaccgc
     2461 gaccgagcca agcagggagg ccagctcgct ggtctgggtg gcatcgagat tggggctcga
     2521 tgatgtaagg gtgctgatgt ccacgacgtt gagggccttc atgaaggcct cctccacccc
     2581 ggtggcgatc ccggccgtgg ccacggcgtc gctcaggatg tcggtgttgg cggggttggc
     2641 cgccaccaag tcggccatcg ccggcatgag ggcagtcgcc aggggaacgt cccggttcgc
     2701 cgtgacgtcg gccgcgtagg tggcggcctg ggtggctgtg aggctcgagg gcacgggggg
     2761 cttggacctc gcgagggagg agccgagcga ggtcgtgaag agttgctggc agcccacaag
     2821 ggtgaggacg aggacgattg agggggcagc gattccggcc cgcagggcct gggatatccg
     2881 catcgaatac ctcccggtac cgagacgccg gacgccctgg gcctcaggga cgggggcgcc
     2941 actaagatta tcggtccgca ggccggcttc caacagggct cagaagcgga tcgccgcctg
     3001 caggaccacg ccagtcctcg gcctgtcccc aggatggagg ccgagctcct cggtgaagac
     3061 ggccccgtcg agcctgagga catagaggtc aaggccgagg ccggccgaga gccagccctt
     3121 gttgatcccg gcccgcgcgg tgatgaagtt caggaaccga agctcggcgc cgacatggag
     3181 gaggctccag gccgactcct gccgcttgta gatcccgacg ggatcctgga cctcgacgta
     3241 aagcgaggga tcgatgaagt gcctgacaag gcggggcttc cacgatgccc cgacggagac
     3301 gatgggcagg ggctgggccc tcgtcgccga gctctcgggc agggcgagcc cgcctccgga
     3361 taccgagctg cccacgctgc cgagggtcgt gctgtccatc atgaaggaag gagagatgtc
     3421 gcggatggac aggccgaagg agaagggctc gaggtcgagg ctggctccga agtccatggc
     3481 caggccgaag ccggcggtga cggggttgtc catgacattc ccgctccccg tgatgaggtc
     3541 gaggaggctc cagcccccgg ggctcgagtc gatgcggtag aagggcctgg cgtcgccgcc
     3601 gatggcgagc ttcaggtcgc cgatcctgat cggggtccca aggccaatca cggcgttcac
     3661 cgaggtcgag ctcgtcatcc tcacgcccat gatcgtgtca cccgaggcgt agttgtcggt
     3721 gacggcaaag aagccgaggc cgacaccctt gcccgtgtag cccaagcccg ccatggctcc
     3781 gccaccgaag ccgttctcga tcagcatgtc gttcacactc gagaccgtct gcgattcggt
     3841 gttcttgccc tggatgatgc gcaggagcct ggacgtgttg gctgaagtgg gcttgaaata
     3901 ggcccatgcc gcgaggtcga ggacggtgaa gtcggcggag ggggacgcga atcccgctgg
     3961 gttgccgtag aatgaagaga agccgctgga cagggcgctg aagctcccgc cgagccccat
     4021 ggccttcgcg tccttggggg cgatgtaggt gaagccctgg gcgagggccg gggcggcggc
     4081 gatgaggcag aggaagaggc ggacgatcgg atgcttcatt gtgcccccac ctagatcccg
     4141 tacttgctga ggtcgagccc ggcggccgcg acgatggcgc cgaggctcgt gccgctcgag
     4201 gtcgatgggg gagtgtagga cgatagcgga gtggcgaggg tcatggcgtc ccacagggca
     4261 tcggccttgt cctgggggct gtccgcccct ccgccgggga ctgctgccac gaaggccgcg
     4321 acgatcgcgg cctggacgga gtccgcggcc ggcatggccc cttgagagcc cgaggcgccg
     4381 atgctggtcc ccatggtgtc gtaggccgcg tcggcggcgc agag
//
LOCUS       DASSQV010000033         5686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1875899, whole genome shotgun sequence.
ACCESSION   DASSQV010000033 DASSQV010000000
VERSION     DASSQV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875899"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /gene="dctP"
                     /locus_tag="VFL04_00575"
     CDS             complement(<1..441)
                     /gene="dctP"
                     /locus_tag="VFL04_00575"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET7838220.1"
                     /translation="MNSIRLRILVLVVLGLALVGAASAQIELKLGHYNAESHPMHTAA
                     VAFADNVAARTGGAVKVTIFPNSKLGSSQEMLEQATLGALDIVIPTDSAVAKYSTKFN
                     LVGAPFAFKDYAATDRFYAGDFLTWVNPDIEQAGLKNLARGEFGF"
     gene            636..1889
                     /locus_tag="VFL04_00580"
     CDS             636..1889
                     /locus_tag="VFL04_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7838221.1"
                     /translation="MSNGDRLERSHMLLILLVSTASFFEGYDFIILNLILPFLQRDFG
                     LDNVRIGLAVSAIALGTIAAFFIIRLGDRFGRRALLIWTVLGYTVATALTAFTRGIYS
                     FVALQFVARAFLVAEWGISTVIVAEEVPAARRGLGISIVQAAAGVGGIVGSAIFPLFL
                     KTAMGWRSMYLVGLVPLVVVFFIRTSVKETRRFSQLKTQAADAPSFSEILAKPQRGKL
                     VLVALLWCFMYLGYQAVYTFYVKFAMDERGLSPGQVSLIAAVAFTLGLGGFIAAGRLM
                     DGWGRRPTAIAFFGAGGICTALAFEAPASLLGPALVVLIFFMTSYLTICGTYTAELFP
                     TRLRASAAAWTNNTLGRVGMVLSPTLVGLLSVPLGGIGPAVSLMGLFPVLCALIVLFF
                     LPETKKRELEVTGELAIEGAGGSRP"
     gene            complement(1962..3494)
                     /locus_tag="VFL04_00585"
     CDS             complement(1962..3494)
                     /locus_tag="VFL04_00585"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7838222.1"
                     /translation="MGKARILIVEDEALSALAIKSYLEDCGFSVGEVLSSGEEAVAAA
                     AETEPDVVLMDIKLDGPIDGVEAAERITKGLRIPVIFLTAYSDSETLRRASLTEPFGY
                     VVKPIDERALSAAIETAIYKASMDRELRATKEKQETILRCVGDGIAVADLHGSVEYLN
                     PAARRLLGFGDSEPLAGVSLFKLFPLSDGKTGESLFLPLQRVVIDGQTVGISDASLAI
                     SGGMKTAVDIDLAPLKDDRGSPRGVVMAFRDVSERRKMQAMVSKELLEATDLQMRLLP
                     ESGRRIGGLEMRGFLRPATFGAGDIYDFYRIDEKTAAFYILDVVGHGISAAGISVLIH
                     RLLSPDPTLPRRLPIIGADAGDPRQVVDRLNELFDVQKDQTFFSLCYGSIELLSGATR
                     LVRAGHLPPVLQSPDGSLRELHSSGAAVGVMKPSDAVELDFVLGPGERLFLYSDGLIE
                     CSDAQSERFSKDRLLGLFEGTRPKPLADAVGRIEAGVAQWRGRTWFDDDLALMAIESG
                     AG"
     gene            complement(3751..4329)
                     /locus_tag="VFL04_00590"
     CDS             complement(3751..4329)
                     /locus_tag="VFL04_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838223.1"
                     /translation="MSFPESAIDDRLVTDAERTAFSLMSELVAAADGVRTRYAIQLAD
                     IMGNPKEFQHYVVGSSADRLARMDSLLRIFTENVKLLVHKTWVEKSEEKPKERLLDEL
                     GAFERDFRDGAIAPAFRRFVALTHSIANLLFGAQSRAEDFLMYCFRIDPKLGLFFWFV
                     GELERQVRAAAAPQEDLMTTEILVGVYVLSSF"
     gene            4444..>5686
                     /locus_tag="VFL04_00595"
     CDS             4444..>5686
                     /locus_tag="VFL04_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HET7838224.1"
                     /translation="MNDIKFLPEDDYGLSFVPKDYLAPGEDEYRIRDVQLEAFGRHPG
                     SFRRLKPYEIETLVKNFNTCSDWNLIRVTDPFTPEMIKNSEFAGLVRIGKLERVTLEH
                     HDLRTTAGITNSRVIACDIGDNCAIHYSTYLAHYLIGDGCILMNNDEIQVSNHAKFGN
                     GVIMEGESEEVRIELDLMNEAGGRSVRPFAGMICADAYLWAKRRDDPELMAAFRAMTD
                     SMFDRRRGRYGTIGRFSVLKSNGVIKDVAIGESAYIKGSNKLKNLTILSSESERTQIG
                     EGVELVNGIVGYGCRVFYGCKAVRFVMGSNSALKYGARLIHSVLGDNSTVSCCEMLNN
                     LIFPAHEQHHNTCFLVASLLKGQSNMAAGATIGSNHNSRANDGEIEAGRGFWPGLSTS
                     VKHSSRFASFCLLAKADYRYEM"
BASE COUNT          900 a         1910 c         1912 g          964 t
ORIGIN      
        1 gaagccgaac tccccgcgcg cgaggttctt gagcccggcc tgctcgatgt cggggttgac
       61 ccacgtgagg aagtcgccgg cgtagaagcg gtcggtggcg gcgtagtcct tgaaggcgaa
      121 gggggcgccg acgaggttga acttggtcga gtacttggcg acggccgagt cggtggggat
      181 gacgatgtcg agggcgccga gggtggcctg ctcgagcatc tcctgggagg agccgagctt
      241 ggagttgggg aatatggtca ccttgaccgc cccgccggtg cgcgccgcga cgttgtcggc
      301 gaaggcgacg gcggcggtgt gcatcgggtg gctctcggcg ttgtagtggc cgagcttgag
      361 ttcgatctgg gcgctggccg cgcccacgag ggcgagtccg aggacgacca gcaccaggat
      421 cctgaggcga atcgagttca tggaagacct ccagcggggc gggcgaccgg cgcctgcctt
      481 ttcgtgatga tgcgtcactc tacgccagat ttccgaggga ggcaagtttt tttgaaacac
      541 gggtccatta ttccgccgac ggcccgacct tgcggcgatg gggcccgggg ccttagtatt
      601 ggtcccgcgc cacgatgccg caggaaggaa ggcccatgtc aaacggcgat aggctggagc
      661 gctcgcacat gctcctcatt ctgctcgtct cgaccgcgag cttcttcgaa ggctacgact
      721 tcatcatcct gaacctcatc ctccccttcc tccagcggga tttcggcctg gacaacgtca
      781 ggatcggcct cgccgtctcg gccatcgcgc tcgggaccat cgcggccttc ttcataatcc
      841 ggctcggcga ccgcttcggg agacgggccc tcctcatctg gacggtcctc ggctacacgg
      901 tcgccacggc cctcacggcc ttcacccggg gcatctactc cttcgtggcc ctccagttcg
      961 tcgcgagggc cttcctcgtc gccgagtggg ggatctcgac ggtcatcgtg gccgaggagg
     1021 tgccggcggc gcggcggggc ctcgggatct cgatcgtcca ggccgcggcc ggggtgggcg
     1081 gcatcgtggg gagcgccatc ttccccctct tcctcaagac cgccatgggc tggcgctcga
     1141 tgtacctggt gggcctcgtg cccctcgtcg tggtcttctt catcaggacc tcggtcaagg
     1201 agaccaggcg cttctcccag ctcaagaccc aggccgccga cgcgccctcc ttctcggaga
     1261 tcctcgccaa gccccagcgc ggcaagctcg tcctcgtggc cctcctgtgg tgcttcatgt
     1321 acctcggcta ccaggcggtc tacaccttct acgtcaagtt cgcaatggac gagcgcggcc
     1381 tctcccccgg ccaggtgagc ctcatcgccg cggtggcctt caccctgggc ctcgggggct
     1441 tcatcgctgc gggcaggctc atggacggct ggggcagacg gcccacggcc atcgccttct
     1501 tcggggccgg cggcatctgc acagccctcg ccttcgaggc cccggcctcc ctgctcggcc
     1561 ccgccctcgt ggtcctgatc ttcttcatga cctcgtacct gacgatctgc ggcacctaca
     1621 cggccgagct cttcccgacc cggctccgcg cgagcgcggc ggcctggacg aacaacaccc
     1681 tggggcgagt tggcatggtg ctctcaccga cccttgtggg cctcctctcg gtgcccctgg
     1741 gcgggatcgg ccccgcggtg agcctcatgg ggctcttccc cgtcctctgc gccctgatcg
     1801 tgctgttctt cctgcccgag accaagaagc gcgagctcga ggtcacgggc gagctcgcca
     1861 tcgagggggc ggggggctcg aggccctagc cggcgccgcg gcctaggggc gccggggctc
     1921 cagccggcgc cctagcccac gccgaaggct tcggggcggc cctagcccgc cccggactcg
     1981 atcgccatga gggccaggtc gtcgtcgaac caggtcctgc ccctccattg ggcgactccg
     2041 gcctcgatgc ggcccaccgc gtccgccagc ggcttgggcc tcgtgccctc gaaaaggccc
     2101 agcagcctgt ccttggagaa gcgctcggac tgggcgtccg agcactcgat gaggccgtcg
     2161 gagtagagga agaggcgctc gcccgggccg aggacgaagt ccagctcgac ggcgtccgag
     2221 ggcttcatga cccccacggc cgccccgctc gagtggagct ccctgaggct tccgtccggg
     2281 gactggagga cgggggggag gtggccggcc ctgacgagcc tggtggcccc gctcaggagc
     2341 tcgatgctgc cgtagcagag cgagaagaag gtctggtcct tctgcacgtc gaagagctcg
     2401 ttgagcctgt cgaccacctg gcgggggtcg ccggcgtcgg cgccgatgat ggggaggcgc
     2461 cggggcaggg tcgggtcggg ggagagcagc ctgtggatga ggacggagat ccccgccgcc
     2521 gagatgccgt ggcccacgac gtcgaggatg tagaaggccg ccgtcttctc gtcgatccgg
     2581 tagaagtcgt agatgtcgcc ggcgccgaag gtcgccggcc tcaggaagcc ccgcatctcg
     2641 aggccgccga tcctcctacc cgactcgggc aggagccgca tctggaggtc ggtggcctcg
     2701 aggagctcct tcgagaccat agcctgcatc ttgcgccgct ccgagacgtc cctgaaggcc
     2761 atcacgacgc cgcggggcga gccgcggtcg tccttgagcg gggcgaggtc gatatcgacc
     2821 gcggtcttca tgcctcccga gatggcgagg ctcgcgtccg atatgcccac cgtctggccg
     2881 tcgatcacga cgcgctggag gggcaggaag agggactccc ccgtcttgcc gtcggacagg
     2941 gggaagagct tgaacagcga gacgccggcg aggggctcgc tgtcgccgaa gccgaggagg
     3001 cggcgggctg cggggttcag gtactcgacc gagccgtgga ggtcggcgac ggcgatgccg
     3061 tcgcccacgc agcgcaggat ggtctcctgc ttctccttcg tcgcgcgcag ctcgcggtcc
     3121 atcgaggcct tgtagatggc ggtctcgatc gcggccgaga gggcccgctc gtcgatgggc
     3181 ttgacgacgt agccgaaggg ctcggtgagg ctcgcccgcc tcagggtctc ggagtccgaa
     3241 taggcggtga ggaagatgac ggggatgcgc aggcccttgg tgatccgctc ggccgcctcg
     3301 accccgtcta tggggccgtc cagctttatg tccatgagga cgacgtcggg ctcggtctcg
     3361 gccgcggcgg cgacggcctc ctcgcccgag gagaggacct cgcccacgct gaagccgcag
     3421 tcctcgaggt aggactttat cgccagggcc gagagggctt cgtcctcgac tatcaggatc
     3481 cgcgcttttc ccatgcctgc tctcccttgg gaggccccgg ggccctgccc ccccggtcgc
     3541 cgtgcttcgg cgctggatgg ggcttcctcg atcgggtctc gctcagcaag aggaacactt
     3601 ttgggaatgg cagttcacga ctatgcgcgc ctacttgacc cttgcggcct tagccggcgc
     3661 agccgttatt aaaccatacc ccggcacagg cctgccgtca agcaaagaac ggccggatga
     3721 ggagggaggg agctggcttg gggcggctct tcagaagctc gagagcacgt agacgcccac
     3781 gaggatctcg gtggtcatga ggtcctcctg cggcgccgcc gcggcgcgga cctggcgctc
     3841 gagctcgccg acgaaccaga agaagaggcc gagcttgggg tcgatgcgga agcagtacat
     3901 gaggaagtcc tcggcccggc tctgggcgcc gaagaggagg ttggcgatgg agtgggtgag
     3961 ggcgacgaag cggcggaagg cgggggcgat ggccccgtcg cggaaatccc gctcgaaggc
     4021 gccgagctcg tcgagcaggc gctccttggg cttctcctcg gacttctcaa cccaggtctt
     4081 gtgcacgagg agctttacgt tctcggtgaa gatgcgcagg agggagtcca tcctcgcgag
     4141 gcggtcggcc gaggagccga caacgtagtg ctggaactcc ttgggattgc ccattatgtc
     4201 agcgagctgg atcgcatagc gggtccggac gccgtcagcg gcggcgacga gttcggacat
     4261 gagactgaag gccgtcctct ccgcgtcggt gacgaggcga tcgtcgatcg ctgattccgg
     4321 aaaactcatg gcctaccatg tcaagaaagc cgttatttgg tcaagtggcc gacccgcctc
     4381 cggaaagcgg cctcagccgg gccgcttgtg gaaggccccg gaccgtggca ttgtgtgctc
     4441 atgatgaacg atatcaaatt ccttccggag gacgactacg gcctgtcctt cgtcccgaag
     4501 gactacctcg ccccgggcga ggacgaatac cgcatccggg acgtccagct cgaggccttc
     4561 ggccgccatc ccgggtcctt ccgccggctc aagccctacg agatcgagac cctcgtcaag
     4621 aacttcaaca cctgctcgga ctggaacctc attagggtga ccgacccctt cacccccgag
     4681 atgatcaaga acagcgagtt cgccggcctc gtgcgcatcg gcaagctcga gcgggtcacc
     4741 ctcgagcacc acgaccttcg gaccacggcc ggcatcacga actcgcgcgt catcgcctgc
     4801 gacatcggcg acaactgcgc catccactac tccacctacc tcgcccacta cctcatcggc
     4861 gacggctgca tcctcatgaa caacgacgag atccaggtgt cgaaccacgc gaagttcggc
     4921 aacggcgtca tcatggaggg cgagagcgag gaggtcagga tcgagctcga cctcatgaac
     4981 gaggcgggcg ggcgctcggt ccggcccttc gcgggcatga tctgcgccga cgcctacctg
     5041 tgggcgaagc gccgcgacga ccccgagctc atggccgcct tccgcgccat gaccgactcg
     5101 atgttcgacc gcaggcgggg gcgctacggc accatcggcc gcttctccgt cctcaagtcc
     5161 aacggcgtca tcaaggacgt ggccatcggc gagagcgcgt acatcaaggg ctcgaacaag
     5221 ctcaagaacc tcacgatcct ctcgagcgag agcgagcgca cccagatcgg cgagggggtc
     5281 gagctggtca acggcatcgt gggctacggc tgcagggtgt tctacggctg caaggccgtg
     5341 cgcttcgtca tgggctcgaa ctcggcgctc aagtacggcg cccgcctcat ccactcggtc
     5401 ctcggcgaca actccacggt ctcctgctgc gagatgctca acaacctgat cttccccgcc
     5461 cacgagcagc accacaacac ctgcttcctc gtggcgagcc tcctcaaggg ccagtcgaac
     5521 atggcagccg gcgcgaccat cggctccaac cacaactcgc gggccaacga cggggagatc
     5581 gaggcgggcc gcggcttctg gcccggtctc tccacctcgg tcaagcactc gtcgcgcttc
     5641 gcctctttct gcctcctcgc caaggccgac tatcgctacg agatgg
//
LOCUS       DASSQV010000034         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_758203, whole genome shotgun sequence.
ACCESSION   DASSQV010000034 DASSQV010000000
VERSION     DASSQV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758203"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFL04_00600"
     CDS             <1..627
                     /locus_tag="VFL04_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7838225.1"
                     /translation="PIIATLILSVAGRGIAMVTTNAMIVWLYAKPFAILGQGYLLGLP
                     FSIFIVLGILLLTALATRRTALGLFIEAVGINPTAARFAGINAKNILFGVYTFSGLCA
                     GISGLVVCSTVMSADGNNAGLGYELSAILAVVLGGTSLNGGKFYLLGSMVGALIVQTL
                     TTTIYAFGVPPEIAKIVIALVVLVVSLLQSSRFRSMFADALGKRGYSV"
     gene            624..1643
                     /gene="yjfF"
                     /locus_tag="VFL04_00605"
     CDS             624..1643
                     /gene="yjfF"
                     /locus_tag="VFL04_00605"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF008630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HET7838226.1"
                     /translation="MNARIGIKARNLPTLVTLGLFILMFGLGSVSFRGFLAPQNFLNL
                     FIDNSYLLILGVGMTFVIISGGIDLSVGSMLAFSTMLSSSLLEKSHMNPLFVIPIVLL
                     VGLSLGTIMGFIIQTFDLPPFIITLAGLYLARGLCYVISIDTIGITNDWWQAVAHAQI
                     KVTGDSFVSVSVIVAAVIVAAGIFVSRSTKFGRTVYAIGGNEQSAILMGLPVARTKIL
                     IYAFSGFCSALAGIVFTFYTLSGYALNGANMEMDAIATVVIGGTLLTGGVGNVLGTVF
                     GVMVYGTIQVLIVFQGTLNSWWTRIGIGFLVFLFCLMQRLFEARKGARGRKRSSGPAA
                     APAGG"
     gene            complement(1725..>2187)
                     /locus_tag="VFL04_00610"
     CDS             complement(1725..>2187)
                     /locus_tag="VFL04_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838227.1"
                     /translation="LGTIFFVANLLAGFSAPASALIAKRIGLVNTMVFTHIPSNLLLI
                     LVPLMPSPGLAVAVLLARFAISQMDVPTRQSYVMSVVDPDERSAAAGVTGIARTTGAS
                     ISPAISGQLIANPAIAGLPFFLAGSLKIVYDLLLYRSFSRAPQAAGEKARR"
BASE COUNT          340 a          763 c          670 g          414 t
ORIGIN      
        1 ccgatcatcg cgaccctcat cctctcggtc gcgggccggg gcatagcgat ggtgaccacc
       61 aacgcgatga tcgtctggct ctacgcgaag cccttcgcca tcctcggcca gggctatctc
      121 ctgggcctgc ccttctcgat attcatagtc ctcggcatcc tcctcctcac cgccctggcg
      181 acgcggagga cggccctcgg cctcttcatc gaggccgtcg gcatcaaccc cacggcggcc
      241 cgcttcgccg gcatcaacgc caagaacatc ctcttcggcg tctacacctt ctccgggctc
      301 tgcgccggga tctcgggcct ggtcgtctgc tccaccgtca tgagcgccga cggcaacaac
      361 gccgggctcg gctacgagct ctcggcgatc ctcgccgtgg tcctgggggg cacctcgctg
      421 aacgggggca agttctacct cctcggcagc atggtcgggg ccctcatcgt ccagaccctg
      481 acgacgacga tctacgcctt cggcgtgccg cccgagatcg ccaagatcgt catcgccctg
      541 gtcgtgctcg tggtcagcct cctccagtca tccaggttcc gctcaatgtt cgcggacgcg
      601 ctcggcaaga gggggtactc ggtatgaacg cgagaatcgg catcaaggcg aggaacctcc
      661 cgaccctcgt gaccctggga ctcttcatcc tcatgttcgg gctgggctcg gtgtccttcc
      721 gcggcttcct cgcgcctcag aacttcctca acctcttcat cgacaactcc tacctcctga
      781 tcctgggcgt cggcatgacc ttcgtcatca tctcgggagg catcgacctg tcggtcggtt
      841 cgatgctcgc cttctcgacc atgctctcct cgagcctcct cgagaagtcc cacatgaatc
      901 cgctcttcgt catcccgatc gtcctcctcg tggggctctc gctcggcacc atcatggggt
      961 tcatcatcca gaccttcgac ctgccgccct tcatcatcac cctggccggc ctgtatctcg
     1021 cccgggggct ctgctacgtc atcagcatcg acaccatcgg gatcacgaac gactggtggc
     1081 aggcagtggc ccacgcgcag ataaaggtca ccggggacag cttcgtcagc gtgagcgtca
     1141 tcgtcgcggc cgtcatcgtg gccgcgggga tcttcgtctc gcgctccacc aagttcggca
     1201 ggacggtcta cgccatcggc gggaacgagc agtcggccat cctcatgggc ctgccagtgg
     1261 cgaggacgaa gatcctcatc tacgccttct cgggcttctg ctcggcccta gcgggcatcg
     1321 tgttcacctt ctacaccctc tccggctacg ccctcaacgg ggcgaacatg gagatggacg
     1381 ccatcgccac cgtcgtcatc ggcggcaccc tcctcacggg aggggtcggc aacgtcctgg
     1441 gcacggtctt cggggtcatg gtctacggga ccatccaggt cctcatcgtc ttccagggta
     1501 ccctgaactc ctggtggacc cggatcggca tcggattcct ggtcttcctc ttctgtctga
     1561 tgcagaggct cttcgaggcc cgcaagggcg caagggggag gaagcgctca tccggccccg
     1621 ccgccgcccc cgcggggggc tgaggcacca ggcccatgcg ctagacgccg ctcgaccgtt
     1681 tccagccccc gggttttgca tctccttggg cccgggggct ttttctatcg ccgggccttc
     1741 tcccccgcgg cctggggcgc cctcgagaag ctcctgtaca gcaggaggtc gtacacgatc
     1801 ttgagggagc cggcgaggaa gaagggcagg ccggcgatgg ccgggttggc gatgagctgg
     1861 cccgatatgg ccggcgatat cgaggcgccc gtcgtcctcg cgatgcccgt gaccccggcc
     1921 gccgccgacc gctcatcggg atcgaccacc gacatgacat aggactgcct cgtcggcaca
     1981 tccatctggg agatggcgaa gcgcgcgagg aggacggcga cggcgaggcc ggggctcggc
     2041 atgaggggca cgaggatgag gaggaggttc gaggggatgt gggtgaatac catcgtgttc
     2101 acgaggccga tccgcttcgc tatcagggcc gaggccgggg ccgagaagcc cgcgagcagg
     2161 ttggcgacga agaagatcgt ccctagg
//
LOCUS       DASSQV010000035         5155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1503635, whole genome shotgun sequence.
ACCESSION   DASSQV010000035 DASSQV010000000
VERSION     DASSQV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503635"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..313
                     /gene="tatA"
                     /locus_tag="VFL04_00615"
     CDS             125..313
                     /gene="tatA"
                     /locus_tag="VFL04_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7838228.1"
                     /translation="MIGTTELIVIVVVILILFGSAAIPKFARSLGEAKKEFTKAMKEG
                     EAESKETGSKEKQAGESK"
     gene            339..1091
                     /gene="tatC"
                     /locus_tag="VFL04_00620"
     CDS             339..1091
                     /gene="tatC"
                     /locus_tag="VFL04_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET7838229.1"
                     /translation="MASSSNPEKVMTVLDHIRELRNRILGSMIALVVGFVVAFIFSDR
                     VIALFTQPFIAVPSAVDKKLVMHSISEGFTTQVKIAAIAGLILSMPVHVFNLVGFVFP
                     GLSRRQRSLVLVMLAASFVLILFGVYIGYFNIVPMAIAFLTNPYFMPPGVGLLLNYET
                     NVFYVLTFMLWSILALQTPLILEVLLIMNVLNRRKVLKASRFVIVGIFIFAAIVTPSP
                     DFISQLGVAIPLTVLYFLAILVAKVFRFGEGR"
     gene            1088..1519
                     /locus_tag="VFL04_00625"
     CDS             1088..1519
                     /locus_tag="VFL04_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838230.1"
                     /translation="MMGIGFSELVVILLVVLVFIRPEDLPAFFRKLGRIYAELKKGYD
                     ELTAVKDEFIREVEAAAKVEDGQRQEPAPGEAASPPPGEAGERGGPEPAPAPVAEASS
                     ETGESDGAQAAHTPAAPAAKTRKPRKSPKTGEGLARPGQPL"
     gene            1529..2047
                     /locus_tag="VFL04_00630"
     CDS             1529..2047
                     /locus_tag="VFL04_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HET7838231.1"
                     /translation="MEKLHFTYQDIHRTARDIAERVRASGFDPHIIVAIGSGGFIPAR
                     ILRTFLGKPILAVGIAYYDDEDRPTDLPRKVQWIEEAERKLSGKRILLVDEVDDSRTT
                     LEYCIRELMRHEPAEIAVAVLHDKDKEKRGIIPPEVSRYFAGLRIEDRWVCYPWDAED
                     IDAHDSAAAGGA"
     gene            complement(2079..2843)
                     /locus_tag="VFL04_00635"
     CDS             complement(2079..2843)
                     /locus_tag="VFL04_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HET7838232.1"
                     /translation="MAKKSTGEPRRKRKEEPEKPQNAERWLLTYADMITLLMVFFVVM
                     YALSKVEVTKFNALAESLSTAFNMPGIQLEEGQGGHSLSPTDAPLRPPPGTGLSPRPS
                     AKRDPFIEKARTTLMSEIRTGSIQISTEARGIVLALSGDVFFREGSATLNEDAINMLE
                     KVAELIVPLPNPIAVEGHSDNTPTARTDRYGSNLMLSAARAVSVAQTLELLNVPRDRL
                     SATGYGDAKPARPNDTAEGRAQNRRVEILIRFDANR"
     gene            complement(2847..3635)
                     /locus_tag="VFL04_00640"
     CDS             complement(2847..3635)
                     /locus_tag="VFL04_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HET7838233.1"
                     /translation="MNLASVLGIPVGFGCLLIAFLIEGGNPASLVLPSPFIIIFGGVF
                     GALLLSFDMSDVMAIPKLIGVALRMPTTREKDLALRFVALAEKSRRDGLLSLEEDMQG
                     LDAHEDALLKKGLRYVIDGTDSDALHELLENDIAIFEKHRKHEVEIFEAAGGVSPTMG
                     IIGTVLGLVIVLSKLNEPEKLGESIAVAFIATLLGIAMANLVFLPLANKLKVVLAKEK
                     NVKEMIVAGVLAIQQGESPKLVGDKLAAFVSEKDRAALESFNEA"
     gene            3827..4231
                     /locus_tag="VFL04_00645"
     CDS             3827..4231
                     /locus_tag="VFL04_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7838234.1"
                     /translation="MSVSMRGRSTGTHTVELIHESGSVIHTMAPKDNGGDGSSFSPTD
                     LCAVSLGACSTTIMNQYAANHGISLRNIEFELVKEMSPPPRRIERLTVTYRIMGVVSD
                     LEFQKVVAAGKACPVRITLGDRVEIVERYERA"
     gene            complement(4228..>5155)
                     /locus_tag="VFL04_00650"
     CDS             complement(4228..>5155)
                     /locus_tag="VFL04_00650"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET7838235.1"
                     /translation="SPPTPKAGLDAALVYCGYGFPEDFASAHAKGAIVLVLRGRAWLR
                     EKAANAAAAGALGLVVFDPERTEFQGTLLAPASIPCAAIGGAEGRALQGRLEAGERIR
                     AHFVVDSSIENGKSRNLIARLPGSRGGKPVLVGAHLDSVFTPGALDDASGLACLLELA
                     RVLAAQAGRAGPARSDIYFVCFGAEELGDLGSTRFVRTYRGPELGGMLELDTVGSGST
                     TMVYTRRGDSNAVAKAALAAAAAIGARAEAGASEGSDHVPFAEAGIPAAFLMREPETR
                     YHSDADQVSAVSEDALVETLRIAAATTLGLAR"
BASE COUNT          876 a         1687 c         1738 g          854 t
ORIGIN      
        1 ccaaggtggg aatccgggac aagccccgaa aaaatcctga tccccgaacc cgaaggacag
       61 cccgaatagg ggccttgagt gaggtcgatc agaacaagag accggttttt gcaaggagta
      121 tgccatgatc ggaaccaccg agctcatcgt catcgtcgtc gtgatcctca tcctgttcgg
      181 atcggccgct atccccaagt tcgcgcgctc cctcggcgag gcgaagaagg aattcaccaa
      241 ggcaatgaaa gaaggagagg ccgagtccaa ggagaccggt tccaaggaga agcaggccgg
      301 ggagtccaag taaagccgac ggcagtctgg aaagagcgat ggcctccagc agcaaccccg
      361 agaaggtgat gaccgtcctg gaccacatcc gggagctcag aaaccggatc ctcggctcga
      421 tgatcgccct tgtcgtgggc ttcgtcgtcg ccttcatctt ctccgaccgg gtcatcgccc
      481 tcttcaccca gcccttcatc gccgtgccga gcgccgtcga caagaagctg gtgatgcaca
      541 gcatctccga gggcttcacg acccaggtga agatcgccgc catcgccggc ctcatcctct
      601 ccatgcctgt gcacgtcttc aacctcgtcg gcttcgtctt ccccggcctc agccgcaggc
      661 agcggagcct cgtcctcgtg atgctggcgg ccagcttcgt gctgatcctc ttcggcgtct
      721 acatcggcta tttcaacatc gtccccatgg ccatcgcctt cctgaccaat ccctacttca
      781 tgccgccggg ggtcggcctc ctgctcaact acgagaccaa cgtcttctat gtcctcacct
      841 tcatgctctg gtccatactc gccctccaga ccccactcat cctcgaggtc ctcctcatca
      901 tgaacgtcct gaaccggagg aaggtgctca aggccagccg tttcgtcatc gtcggcatct
      961 tcatcttcgc ggcgattgtg accccctccc ccgacttcat cagccagctg ggcgtcgcca
     1021 tacccctgac tgtcctctat ttcctcgcga tcctcgtcgc gaaggtcttc aggttcggag
     1081 aaggccgatg atggggatcg gtttctccga gctggtggtc atcctcctgg tggtcctcgt
     1141 cttcatcagg ccagaggacc ttcccgcctt tttccggaag ctcggcagga tctacgccga
     1201 gctgaagaag ggctatgacg agctcacggc cgtcaaggac gagttcatca gggaggtcga
     1261 ggcggcggct aaggtcgagg atgggcagcg gcaggagccc gcgccagggg aagcggcctc
     1321 gccgccgcct ggggaggccg gggagagagg cgggccagag ccagccccgg cgccagtggc
     1381 cgaggcctcc tcggagacgg gggaaagcga cggggcccag gcggcccata cgcccgcagc
     1441 ccctgcggcc aagaccagga agccgaggaa gagcccaaag acgggcgaag gccttgcgcg
     1501 acccggccag cctctctagt atccgtccat ggagaagctt cacttcacat accaggacat
     1561 acacaggacg gcgcgggaca tcgccgagcg cgtgagggcg agcggcttcg acccgcacat
     1621 catcgtcgcc ataggctcgg gcggtttcat ccccgcccgg atactccgca ccttcctcgg
     1681 caagcccatc ctcgcggtgg gcatagccta ttacgacgac gaggacagac cgaccgacct
     1741 cccccgcaag gtccagtgga tcgaggaggc cgagcgcaag ctctcgggca aacgcatcct
     1801 cctcgtcgac gaggtggacg attcgcggac caccctcgag tactgcatcc gcgagctcat
     1861 gcgccacgag cccgccgaga tcgcggtggc cgtcctccac gacaaggaca aggagaagcg
     1921 gggcatcatc ccgcccgagg tctcgcgcta cttcgccggc ctgcgcatcg aggaccgctg
     1981 ggtctgctat ccctgggacg ccgaggacat cgacgcccac gactcggccg ccgccggcgg
     2041 ggcctaaagc cgcggacggc cgaggccggg ccctgggctc agcggttggc gtcgaagcgg
     2101 atgagtatct cgacccggcg gttctgggcc cgcccctcgg ccgtgtcgtt cgggcgggcg
     2161 ggtttggcgt ccccgtagcc cgtcgccgag agccggtcgc ggggcacgtt cagcagctcc
     2221 agggtctggg ccaccgagac ggccctcgcc gccgacagca tcaggttgga gccgtagcgg
     2281 tccgtcctcg ccgtgggggt gttgtcgctg tggccctcca cagcgatggg gttcggcagg
     2341 ggcacgatga gttcggccac cttctccagc atgttgatgg cgtcctcgtt gagggtggcc
     2401 gagccctcgc ggaagaagac gtcgcccgag agcgcgagga cgatgccccg cgcctccgtg
     2461 ctgatctgga tcgagcccgt cctgatctcg ctcatgaggg tcgtccgcgc cttctcgatg
     2521 aagggatcgc gcttggccga gggcctcggc gaaaggcccg tgccgggcgg cggccttaag
     2581 ggggcgtcgg tgggcgagag cgaatggccg ccctggccct cctcgagctg gatccccggc
     2641 atgttgaagg ccgtggagag ggattcggcc agcgcgttga acttggtgac ctcgaccttg
     2701 gaaagggcat acatgacgac gaagaagacc atgaggaggg tgatcatgtc ggcgtaggtg
     2761 aggagccagc gctcggcgtt ctgcggcttc tcgggctctt ccttgcgctt gcggcggggc
     2821 tcgcccgtgg acttcttggc caccgcctag gcctcgttga aggactcgag ggcggcgcgg
     2881 tccttctcgg acacgaaggc ggccagcttg tcgccgacga gcttggggct ctcgccctgc
     2941 tggatggcga gtaccccggc gacgatcatc tccttcacgt tcttctcctt cgcgagcacg
     3001 accttcagct tattggcgag gggaaggaac acgaggttgg ccatcgcgat gccgaggagg
     3061 gtggctatga aggcgacggc gatggactcg ccgagcttct ccggctcgtt gagcttggag
     3121 aggacgatga cgaggccgag gaccgtgccg atgatgccca tggtgggcga gacgccgccg
     3181 gcggcctcga agatctcgac ctcgtgcttc cggtgtttct cgaatatggc gatgtcgttc
     3241 tccaggagct cgtggagggc gtcggagtcc gtgccgtcga tgacgtagcg aagccccttc
     3301 ttgaggaggg cgtcctcgtg ggcgtcgagg ccctgcatgt cctcctcgag ggacagaagg
     3361 ccgtcgcgcc gggacttctc cgccagggcc acgaacctca gggcgaggtc cttctccctg
     3421 gtcgtgggca tgcgcagggc cacgccgatg agcttcggga tggccatgac gtcgctcatg
     3481 tcgaaggaca gaagcagggc gccgaagacg cccccgaata tgatgatgaa gggcgagggg
     3541 aggacgaggc ttgccgggtt gccgccttcg atgaggaagg cgatgaggag gcaaccgaag
     3601 ccgacgggaa tacccaggac ggaggcgaga ttcatgctgt acctcaggcc atagtatagg
     3661 tcccgcccga gccgagcggc aaggagccgc cgaccctcca agacgggtca ttccctttag
     3721 ttatcggctc gtatggtcag ggatctgact gatatgcggg caaggatgag gatttggtgc
     3781 tactattgcc tccccgccca aggggcggcc agaaggagga agctttatga gcgtatccat
     3841 gcgaggccgg agcaccggca cccacaccgt cgagctgatc cacgagtccg gctcggtgat
     3901 ccacaccatg gcgcccaagg acaacggcgg cgacggctcg agcttttcgc ccaccgacct
     3961 ctgcgcggtg tcgctggggg cctgctccac caccatcatg aaccagtacg cggccaacca
     4021 cgggatctcg ctcaggaaca tcgagttcga gcttgttaag gagatgagcc ccccgccgcg
     4081 gaggatcgag cgcctgaccg tgacctaccg gatcatgggg gtcgtatccg acctcgagtt
     4141 ccagaaggtc gtggccgccg gcaaggcctg ccccgtcagg atcaccctcg gcgacagggt
     4201 cgagatcgtg gagcgctacg agcgggccta gcgcgcgagc ccaagggtgg tcgcggcggc
     4261 gatccgcagg gtctccacga gggcgtcctc cgagactgcc gagacctggt cggcgtcgct
     4321 gtggtacctc gtctcgggct cccgcatgag gaaggcggcg gggatgccgg cctcggcgaa
     4381 tggcacgtgg tcggagccct cggaggctcc cgcctcggcc ctggcgccga ttgcggcggc
     4441 cgcggcgagg gcagccttgg cgacggcgtt cgaatcgccg cggcgcgtgt agaccatggt
     4501 ggtgctgccc gagccgacag tgtcgagctc gagcatcccg ccgagctcgg ggccgcggta
     4561 ggtgcggacg aagcgtgtcg agccgaggtc gccgagctcc tcggccccga agcacacgaa
     4621 gtagatgtcg gaccgcgccg gtccggcccg gccagcctgg gcggcgagga cgcgggcgag
     4681 ctccaggagg caggccagac ccgaggcgtc gtcgagggcc ccgggcgtga agaccgagtc
     4741 gaggtgggcc ccgacgagga ccggctttcc gccgcgggag cccggcagcc tcgcgatcag
     4801 gttcctgctc ttcccgttct cgatgctgct gtcgaccacg aagtgcgccc gtatcctctc
     4861 tccggcctcg agccggccct ggagggcccg gccctcggcc cccccgatcg cggcgcaggg
     4921 tatcgaggcc ggagcgagca gggtgccctg gaactcggtc cgctcgggat cgaagaccac
     4981 aaggcccagg gctcccgccg cggccgcgtt ggccgccttc tcgcggagcc aggccctgcc
     5041 gcgtagcacg aggactatcg ctcccttggc gtgcgcgctg gcgaagtcct cggggaagcc
     5101 gtagccgcag tagaccaggg cggcgtcgag cccggccttg ggcgtcggcg gggaa
//
LOCUS       DASSQV010000036         4332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_758675, whole genome shotgun sequence.
ACCESSION   DASSQV010000036 DASSQV010000000
VERSION     DASSQV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758675"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..891)
                     /locus_tag="VFL04_00655"
     CDS             complement(34..891)
                     /locus_tag="VFL04_00655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET7838236.1"
                     /translation="MTGFAHREIAQKGLRGSMELKSYNNRYLDLSVSLPPYLSRLEPR
                     FREYLAARIVHGKVELWLKVRELDVPVAVSADFEAAKAVALVLKQMSAACSLEEKPRL
                     GNILSFDGVVAYERDVDEDSLWGRIEPELEACFAEYEASRRREGKATEADIEAQLGRL
                     ESVTEAVRAYVPEVERTVKLQIQTRFREVLGDGIDEGRALAETASMLMKYTINEELSR
                     LGAHLASFRRIVGTEAAPGKKLDFLCQEMNREVNTIGSKSILLPVSQAVVELKDALEN
                     VREQLRNIE"
     gene            complement(900..2378)
                     /gene="murC"
                     /locus_tag="VFL04_00660"
     CDS             complement(900..2378)
                     /gene="murC"
                     /locus_tag="VFL04_00660"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET7838237.1"
                     /translation="MQAGPALSSARGMHIHLVGAKGTGMTALAEILSADGAILSGSDV
                     ADVFYTDAILKGIGVQVASPFSEANLGPELDLVVHSAAYRRDLNPELLEAARRGIPVL
                     SYPEALGELSLTRDSSAIAGVHGKTTTTALAGTIAASLAIPATILAGSAVSTFGGRST
                     MIRGSRYFIAETCEYRRHFLSFHPRRIVLTSVEHDHQDYFPSYADILSAFVEFAQTLP
                     AGGLLIYCADDRGACEAVAKVAAGRPDIVLLPYGKTATGDYRISDYRVGQGLAHFRLA
                     GFHREFELRIPGEHIALDATAALALAFSVHRDAKAGAGLEEADVEAARRALASFSGSK
                     RRSEILGSAGDVLFMDDYAHHPTAIREGIRGLKAFWPGRRLVVDFMSHTYSRTKALFG
                     DFAASLDGADAVVLHRIYASARELPEEGVSGRLLFEAVRDRRSRLGIEGEVMYWEDPL
                     DAKSSLHGFLRPGDLFITMGAGDNWRLGAELFDARRNGGKAE"
     gene            complement(2444..>4332)
                     /locus_tag="VFL04_00665"
     CDS             complement(2444..>4332)
                     /locus_tag="VFL04_00665"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HET7838238.1"
                     /translation="EGSYETHFEETYQISWRPFLSVLYRFPDISALLHYSGIVLRWLE
                     SRHPEFLMLLEEMVLRKQIELLGGGFFAPLLPLVPGPDRLGQIELLTTYIRKAFGKRP
                     RGCWLQEYAWEPGLASTIQTCGFDYTFLPERHFRFAGIEGELLGEPSMTEDQGRSIVV
                     FPVFDALESFAEPLPPTEALASLKNRLGELPLYTVLYSSGSTRTMWESSGLESPDVFF
                     ERSFSSLQRASLEYETTTPSRYIKGLRQFGRAYFTGSGSSLLMERSLRPEQQRSGTLV
                     DDDSPSLRRGSPRRLLLRHEESLSLYAKMHYVRILVSQLRGDKSRKKTAQEELWRGQC
                     GDAYWQGATGGILRLPLRAAAYAALIEAEKTTRQRGTFSPGVIRTDIDFDGQKEILYQ
                     GLDINAYVHLQGGCLTELDSFKTRTNYVNVLGPDPERRRRACFRDRIFAAGGGGADRG
                     SFADSQYSLVETDGPAHLAILARDGWCEQEGRRQPLSIRKTFTFRKGGLTVDYELINR
                     DIEMSAFRFAVELNFSAGSDPASVGLSALRGREIVPLESAQVCSADGLTGMRLENLRH
                     SEHIDIRSDTVFDLAHEPVYIDAGQDSGQDGRYQGCRLMCFWEVGLAPEASQRLSITM
                     ELRS"
BASE COUNT          724 a         1352 c         1482 g          774 t
ORIGIN      
        1 tcgctatgat cccgctatgg gtgacttttg ctttcattcg atgttacgca gctgctctct
       61 gacgttctca agggcgtcct tgagctcgac cacggcctgg ctgacgggca ggaggatgct
      121 cttggacccg atcgtattga cttcgcggtt catctcctgg cagaggaagt ccagcttctt
      181 gccgggggcc gcctcggttc cgacgatgcg gcggaaggag gccagatgtg ccccgagccg
      241 cgagagctcc tcgtttatgg tgtacttcat gagcatggac gccgtctcag cgagggccct
      301 cccctcgtct atgccgtctc cgaggacctc cctgaagcgc gtctggatct gcagcttgac
      361 ggtgcgttcc acctcgggga catacgcccg cacggcctcg gtgacggact cgagcctgcc
      421 gagctgggcc tcgatgtcgg cctcggtggc ctttccctcc cgtcttcgcg aggcctcgta
      481 ttcggcgaaa caggcctcaa gctcgggctc gatgcgcccc cagagagaat cctcgtcgac
      541 gtcgcgctca taggctacga ccccgtcgaa ggacaggatg ttgccgagtc gaggcttctc
      601 ctcgagggaa caggcggcgg acatctgctt gagtacgagg gcgacagcct tggccgcctc
      661 gaagtcggcg ctgacggcga cagggacgtc gagctcgcgt accttgagcc agagctctac
      721 cttcccgtgt acgatgcgcg ccgccaggta ctcgcggaac ctcggctcta ggcgggagag
      781 gtagggcgga agggagacgg agaggtcgag atagcggttg ttgtaggact tgagttccat
      841 gctcccgcgc agccccttct gggcgatctc gcggtgcgcg aatccggtca tgctcttgat
      901 cattctgctt tcccgccatt ccttctcgcg tcgaagagtt cggcccccag cctccaattg
      961 tccccggccc ccatcgtgat gaagaggtcg cccggccgga gaaagccgtg gagggaggat
     1021 ttcgcatcca ggggatcctc ccaatacatc acctcaccct cgatgccgag ccgggacctt
     1081 cggtcccgga cggcctcgaa gaggagcctt cccgagactc cttcctcggg aagctcgcgc
     1141 gccgacgcat agatccggtg caggaccacg gcatcggcgc cgtcgaggct cgcggcgaaa
     1201 tcgccgaaaa gggccttggt gcgcgaatag gtatgggaca tgaaatcgac gaccagcctg
     1261 cgaccgggcc agaaggcctt gagccccctg atcccctcgc ggatcgccgt tggatgatgg
     1321 gcatagtcgt ccatgaagag gacgtccccc gccgagccga ggatttccga tcggcgtttg
     1381 gatccggaga aggaggcgag cgcccgccgg gcggcctcga catcggcctc ctccagcccg
     1441 gctccagcct tcgcgtcgcg gtgtaccgag aacgcgaggg ccagggcggc ggtggcatcg
     1501 agggcgatgt gctcgccggg tatgcggagc tcgaactcgc ggtggaagcc ggccaggcgg
     1561 aaatgggcca ggccctggcc cacgcggtag tcggagatgc ggtagtcgcc cgtcgcggtc
     1621 ttgccatagg gcaggagcac gatgtccggt cggcctgccg cgaccttcgc gacagcctcg
     1681 caggcgcccc tgtcgtccgc gcaatagatc agcaggcctc ccgccgggag ggtctgggcg
     1741 aactcgacaa aggccgaaag gatgtcagca tagctgggga aatagtcctg atggtcgtgc
     1801 tcgacgctgg tgaggacgat cctgcgcgga tggaacgaga ggaaatgcct gcggtactcg
     1861 caggtctcgg cgatgaagta cctgctgccg cggatcatgg tggaccttcc gccgaaggtg
     1921 gatacggccg agcccgcgag gatcgtggcc ggtattgcaa ggctcgccgc gatcgtgccc
     1981 gcgagggccg tcgtggtggt cttgccgtgc actccggcta tcgccgagga gtcccgcgtc
     2041 agggaaagct cgccgagggc ctcgggatag ctgaggacgg ggattcccct cctcgcggcc
     2101 tcgagcagct ctgggttgag gtcgcgtctg taggccgctg agtgtactac caggtccagc
     2161 tcgggcccga ggttggcctc cgagaatgga ctcgcgacct ggacgccgat gcccttgagg
     2221 atcgcgtcgg tgtagaagac atcggcgacg tcgctgccgc tcaggatcgc cccgtctgcc
     2281 gatagtatct ccgcgagggc ggtcatgccg gtgcccttag cgccgacgag gtggatgtgc
     2341 atcccgcgag cagagctcag ggctggtccc gcttgcatgt cggcattcta gcgaaagcgc
     2401 atcgggtgga caaggcgcgc cggctccgcg gggcgggggc gcttcaggag cgcagctcca
     2461 tggtgatcga gagccgctgc gaggcttcgg gggccaggcc cacctcccag aagcacatga
     2521 gcctgcagcc ctgatatcgc ccatcttgac cggaatcctg gccggcatcg atgtagacgg
     2581 gctcatgggc gaggtcgaag accgtgtccg agcggatgtc gatgtgctcc gagtggcgga
     2641 ggttctcgag cctcatgccg gtcaggccat cggcggagca gacctgggcc gattcgaggg
     2701 ggacgatctc cctgcctctc agcgccgaga gcccgacgct cgccggatcg gagccggccg
     2761 agaaattgag ctcgaccgcg aagcggaagg ccgacatctc gatgtcccgg ttgatgagct
     2821 cgtagtccac ggtgaggccg cccttgcgga acgtgaaggt cttgcggatg gagaggggct
     2881 gcctccgccc ttcctgctcg caccatccgt cccgcgctag gatcgcgaga tgggcggggc
     2941 cgtcggtctc cacgaggctg tactgcgaat cggcgaaact gccccgatcc gcccctccgc
     3001 ccccggcggc gaatatcctg tccctgaagc aggcccgcct cctccgctcg gggtcgggcc
     3061 cgaggacatt cacatagttc gtccgggtct tgaaggagtc cagctccgtg aggcagccgc
     3121 cctggaggtg gacataggcg ttgatgtcga ggccctggta caggatctcc ttctgcccgt
     3181 cgaagtctat gtccgtcctg atgacccccg gggagaaggt gccccgctgt ctggtggtct
     3241 tctcggcctc gatgagggcg gcgtaggccg cggctcgcag gggcagtctg aggatccccc
     3301 ccgtcgcgcc ctgccaatag gcgtcgccgc actgtccacg ccagagctcc tcctgtgccg
     3361 tcttcttgcg cgacttgtcg ccgcggagtt ggctcaccag gatcctgacg tagtgcattt
     3421 ttgcatacaa ggagaggctc tcctcgtggc ggagcagaag ccgtctgggg ctgccgcggc
     3481 gcagggaggg gctgtcatcg tcgacaaggg tgcccgacct ctgttgctcg ggcctgaggc
     3541 tcctctccat gaggaggctc gaaccgctgc ccgtgaagta ggcgcgtccg aattgccgga
     3601 gtcccttgat gtagcgcgag ggggtggttg tctcgtactc caggctcgcc cgctggaggg
     3661 aggagaagga gcgctcgaag aagacgtcgg gcgattcgag tcccgaggat tcccacatgg
     3721 tcctcgtgct cccgctcgaa tagaggacgg tgtagagcgg cagctcgccg agccgattct
     3781 tcagggaagc cagggcttcg gtggggggca ggggctcggc gaagctctcg agggcatcga
     3841 agacggggaa cacgacgatg ctgcggccct gatcctcggt catcgaaggc tcgccaagga
     3901 gctcaccttc tatgcccgcg aagcggaagt gccgctcagg gaggaaggtg tagtcgaagc
     3961 cgcaggtctg tatcgtcgag gccaggcccg gctcccaagc atactcctgg agccagcagc
     4021 ccctagggcg tttcccgaag gccttcctga tgtaggtcgt cagcagctcg atctgcccca
     4081 gcctgtccgg tccgggcaca aggggcagga ggggggcgaa gaagccgccc ccaaggagct
     4141 ctatctgctt cctgaggacc atctcctcga ggagcatgag gaactcggga tgcctcgact
     4201 ccagccagcg caggacgatg cccgaatagt ggaggagggc cgatatgtcc ggaaagcggt
     4261 agagaacgga aaggaacggg cgccaggaga tctggtaggt ctcctcgaaa tgcgtctcgt
     4321 agctgccttc cg
//
LOCUS       DASSQV010000037         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1876870, whole genome shotgun sequence.
ACCESSION   DASSQV010000037 DASSQV010000000
VERSION     DASSQV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876870"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VFL04_00670"
     CDS             complement(<1..1102)
                     /locus_tag="VFL04_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HET7838239.1"
                     /translation="MRLPARRATAAGPGRAPRLGRALSALAVMAALSGAPLAAASPER
                     LQHYELGVAEGLANNSIYCILQDRRGYMWFGSFGGLSRYDGESFENFRPRADGRSLAA
                     SVVFALLEDGSGSLWVGTDGGGLARYDQDADSFELFRAGSEGAGALSSDRILSLALDS
                     RGRIVAGGGDGTINILDPRTGRVSRIVPPHPAPIRCIASDSSGRIWAGTEGAGILGYD
                     EAKGELRAFAHSPADPSSLGSDTVRCLFLDSGGRLWAGLGNGDVDLVLDGRFAHDRVE
                     GGRPHPAQAVRSLAEDGDGTIWVGYEDSGIGVLDSATMVLRPPSAGGSPSMVRALYRD
                     RKGLMWAGLKEGGLRVYNLRSALFTRYLSLDDG"
     gene            complement(1099..2928)
                     /locus_tag="VFL04_00675"
     CDS             complement(1099..2928)
                     /locus_tag="VFL04_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7838240.1"
                     /translation="MAVSLRIRSRLLLAFLLIALIGAAMGGMGIYFVRALSRQSVTLL
                     ERTSSPLRRIFGLHQAVLEAQVFARDLLLVEGLRQAKIASDLGRRGAFIDGESAALID
                     EVSEEGLKGALGSFRLAWTAFDRELDAFAQEAGKGAKEASAARMYALMDGPGRSLKEL
                     MDQIVDGYLALASSSVERSQALARASTMVLLGLVAAGLGLSFLLAIAMARGISLPLRK
                     AVEAASAIASGNLAVGLDGRFTGRRDEVGELSRALEAMARDLNTGMCTLRSSVDVLDS
                     VGGELEESLASTRQAVARIADSAQAVSRQSLEQAAGVEETAATVRGMTRTIEGLDDRI
                     AVQAEGVSSSSASVEQMVGNIHSVAASVARLGQSFASLMAASEDGRSKLEQVSSLIHE
                     IDVQSDKLMEANAAVSGIASMTNLLAMNAAIEAAHAGETGKGFAVVADEIRGLAESAG
                     AQSSEISGDIAEIRATIDLVVSSSKEASSSFASILGLIGDVSALEREINAALEEQREG
                     SRQVLEGLASINEISGQVRSGSLELREGSGAIAREMAELQTATDVLRQVAEGIGRSVS
                     EIDTRAEAVVESSRRNRSSLDEVEALIGRYSLLEAGSCGDTEE"
BASE COUNT          449 a         1043 c         1159 g          435 t
ORIGIN      
        1 ggccgtcgtc taggctcagg taacgggtga agagggccga gcggaggttg tagacccgaa
       61 gccctccctc cttcaggccg gcccacatga ggcccttgcg gtcgcggtag agggccctca
      121 ccatcgacgg cgagccgccc gcggacggcg ggcggaggac catggtcgcg ctgtccagga
      181 cgccgatgcc cgagtcctcg tagcccaccc agatcgtccc gtcgccgtcc tcggcgaggc
      241 tgcgcacagc ctgggcggga tggggacggc cgccctcgac gcggtcgtgg gcgaagcgcc
      301 cgtccaggac gaggtcgacg tcgccgttgc cgagccccgc ccagagccgg ccgcccgagt
      361 ccaggaagag gcagcgcacc gtgtcggagc cgagggagga gggatcggcg ggcgaatgcg
      421 cgaaggcgcg gagctccccc ttcgcctcgt cgtagccgag gatgcctgca ccctcggtgc
      481 ccgcccagat cctgcccgag gagtcggagg cgatgcagcg gatcggggcg ggatgggggg
      541 gcacgatgcg cgacacccgt cctgtcctgg ggtcgaggat gttgatggtg ccgtcgcccc
      601 cgcccgcgac gatgcgcccc ctcgagtcga gggcgaggga gaggatgcgg tcgctgctca
      661 gggcgcccgc cccctcgctc cccgcgcgga agagctcgaa ggagtcggcg tcctggtcgt
      721 agcgggccag gcctcccccg tcggtcccga cccagagcga gcccgagccg tcctcgagga
      781 gggcgaagac gaccgaggcg gcgagggagc ggccgtcggc ccggggcctg aagttctcga
      841 agctctcgcc gtcgtagcgc gacaggccgc cgaagctccc gaaccacatg tagcccctcc
      901 ggtcctggag gatgcagtag atcgagttgt tggcgaggcc ctcagcgacg ccgagctcgt
      961 agtgctggag ccgctcgggg ctggcggcgg cgaggggggc gccggagagg gcggccatga
     1021 ccgccagggc agacagggcg cggccgaggc ggggcgcgcg gcccgggccg gccgccgtgg
     1081 cacggcgcgc gggaagcctc actcctcggt gtccccgcag gatccggcct ccaggaggga
     1141 gtatcggccg atcagggcct cgacctcgtc cagggagctg cggttcctgc gcgatgactc
     1201 cacgacggcc tcggccctcg tgtcgatctc gctgacgctg cgaccgatgc cctcggccac
     1261 ctgtctgagc acgtcggtgg cggtctggag ctcggccatc tccctggcta tggcgcccga
     1321 gccctccctg agctccaggg agcccgagcg gacctggccc gagatctcgt tgatcgaggc
     1381 gaggccctcg aggacctggc gcgagccctc gcgctgctcc tcgagggccg cgttgatctc
     1441 gcgctcgagg gcgctcacgt ccccgatgag gcccaggatc gaggcgaagg agctcgaggc
     1501 ctccttggag ctcgagacca cgaggtcgat cgtcgcccgg atctcggcga tgtcgcccga
     1561 gatctcgctg gactgggccc cggcgctctc ggccaggccc ctgatctcgt cggcgaccac
     1621 ggcgaagccc ttgcccgtct caccggcgtg ggcggcctcg atggcggcgt tcatggcgag
     1681 gaggttggtc atcgaggcga tgcccgagac ggcggcgttg gcctccatga gcttgtcgga
     1741 ctggacgtcg atctcgtgga tgagggagct cacctgctcg agcttggagc gcccgtcctc
     1801 ggaggcggcc atgaggctcg cgaagctctg gccgagccgg gcgacgctgg cggccacgct
     1861 gtggatgttc ccgaccatct gctccaccga ggccgaggag gagctgactc cctcggcctg
     1921 gacggcgatc ctgtcgtcga ggccctcgat ggtcctggtc atgccgcgca cggtcgccgc
     1981 ggtctcctcg accccggcgg cctgctcgag gctctggcgc gagacggcct gggcgctgtc
     2041 ggcgatgcgg gcgacggcct gccgggtgga ggcgaggctc tcctcgagct cgccgccgac
     2101 gctgtccagg acgtcgaccg agctcctgag ggtgcacatg ccggtgttca ggtccctggc
     2161 cattgcctcg agggcgcggg agagctcgcc cacctcgtcc ctgcgcccgg tgaaacggcc
     2221 gtcgaggccc accgcgaggt tgcccgaggc gatggctgag gccgcctcga cggccttccg
     2281 caggggcagg gagatccccc ttgccatcgc gatggcgagg aggaaggaca ggccgaggcc
     2341 ggcggccacg aggccgagga ggaccatggt cgaggcgcgg gcgagggcct ggctccgctc
     2401 cacgctcgag ctcgcgaggg cgaggtagcc gtcgacgatc tggtccatca gctccttgag
     2461 gctgcggccg gggccgtcca tgagggcata catgcgcgcg gcgcttgcct ccttcgcgcc
     2521 cttgccggcc tcctgggcga aggcgtcgag ctcgcggtcg aaggcggtcc aggcgagcct
     2581 gaaggagccg agggccccct tgaggccctc ctcgctgacc tcgtcgatga gggcggcgga
     2641 ctccccgtcg atgaaggcgc ccctcctgcc gaggtccgag gctatcttcg cctggcgcag
     2701 gccctcgacc aggaggaggt ccctcgcgaa gacctgggcc tcgaggacgg cctggtggag
     2761 gccgaagatg cggcggaggg gagaggaggt ccgctcgagc agggtgacgg actggcgcga
     2821 gagcgcgcgg acgaagtaga tgcccatgcc gcccatcgcg gcgccgatga gggcgatgag
     2881 gaggaaggcc aggaggagcc ttgaccggat cctcagagag actgccatag agcgatctcc
     2941 aggtgccgaa gttgtttggg gcccgctcgg gcgggccggc gaccactcta acccgataat
     3001 ccctctcaga caatgagaga tcgcctccct ggcctcggga ggcggagaat ctcgatatat
     3061 tagaaaagag ctatgaagag aaggcc
//
LOCUS       DASSQV010000038         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_510120, whole genome shotgun sequence.
ACCESSION   DASSQV010000038 DASSQV010000000
VERSION     DASSQV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510120"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFL04_00680"
     CDS             complement(<1..316)
                     /locus_tag="VFL04_00680"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HET7838241.1"
                     /translation="MLMLDSLRQKRRAVSAQRLAEEMGVSLRTVYRDIESLRLLGVPV
                     EGEAGVGYLLRSGYFMPPLVLSAEEIEAVVLGIRWVLDRGDPQLASGARSALSKLKEV
                     LPK"
     gene            complement(367..1371)
                     /locus_tag="VFL04_00685"
     CDS             complement(367..1371)
                     /locus_tag="VFL04_00685"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7838242.1"
                     /translation="MGNIGIYGFGRIGRTMLRAALERKLFVPSHIGDVKDPSTLAALF
                     EVDSNFGRWPEEAAFEDGAIRVGGRRIGFVDTAAAVPDWGALGVDVVVDCSGRGTRRE
                     NAQAHLAKGARKVLVSAGPKSLADCDAVLLPGINLDSYDPKKHHIVSMGSCTTNALAP
                     VVKILRENLGIESGLFSTVHSYTNSQSLTDQPMADRRDSWAAAENLIPSSSGAAKALL
                     FIWPDLPVSGKAYRVPTRTGSIVELVATVGKATDAAAVRQLFREAADSAALRGILGVL
                     EGEYASSRIVGEPRSSIIDLPLTAVMKGRLVSVAAWYDNEYGFSTRLAETAALLASRV
                     "
     gene            complement(1508..1999)
                     /locus_tag="VFL04_00690"
     CDS             complement(1508..1999)
                     /locus_tag="VFL04_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7838243.1"
                     /translation="MADNAPHGLRKSLAQHLDGHGAHISLDDALEGFPFELAGRRPPH
                     LGHSAWGLVYHLWICQWDILEYSLKPSHHSPEYPHGYWPKLDSPRDESEWLSTIDKFR
                     HDLARAHDLVLDPGRGLDVPLRKGIERTPIELAILVTDHNSYHIGQLVDLRTLLGVPV
                     RDW"
     gene            complement(2029..2412)
                     /locus_tag="VFL04_00695"
     CDS             complement(2029..2412)
                     /locus_tag="VFL04_00695"
                     /inference="COORDINATES: protein motif:HMM:NF042576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6717 family protein"
                     /protein_id="HET7838244.1"
                     /translation="MRLRAPGLRDLMRLKGLFVTPEVRAYRDEEGWFIDDDNGFSKSD
                     NELIAGIPKIIELMAGSDARRVRIRYSDRPFKDAKLLSLLHSNELGATYQYDDGELHE
                     GWLCPVFFWYFKEAPARLYVRIRKA"
     gene            2765..>3412
                     /locus_tag="VFL04_00700"
     CDS             2765..>3412
                     /locus_tag="VFL04_00700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET7838245.1"
                     /translation="MNVNIRLETTGDYLAVEELTREAFWNLYNPGCDEHYLCHIMREH
                     RDFIKDLDYVAEVEGKIVGSIMYTKSLLLGDDQMREEIVSFGPICVHPEYQRRGIGTA
                     LIEKTRRLVEKSDIPAIAIYGDPHNYCKHGFRNGIDYGVSSMEGEHPLGLLVLELRPG
                     FFGPRAWRLKQSDVFQFEHEEAVEFDKGFNEKEKRVQYSQEIFQMQIRSFLRAKPA"
BASE COUNT          643 a         1012 c         1160 g          597 t
ORIGIN      
        1 tcttcgggag gacctccttg agcttggaga gggcgcttct cgcgcccgag gcgagctggg
       61 gatcgccgcg gtcaaggacc catctgatcc cgagcacgac cgcctcgatc tcctcggcgg
      121 acaggaccag gggaggcatg aagtagccgg acctgagcag atagcccacc ccggcctcgc
      181 cctcgaccgg cacgccgagg agcctgagcg actcgatgtc ccggtagacc gtcctgaggc
      241 tgactcccat ctcttccgcg agacgctgcg ccgacaccgc ccgccgcttc tggcggaggc
      301 tgtcgagcat gagcatcagc cgctcggcct tggacgtttc ccgcgtagcc aaaccagatt
      361 acgtggctag acgcgcgagg cgaggagggc ggcggtctcg gcgagccggg tcgagaagcc
      421 gtactcgttg tcgtaccacg ccgcgaccga gaccagacgg cccttcatca cggcggtcag
      481 cggcaggtcg atgatcgagg agcggggctc cccgacgatc cgagatgagg cgtactctcc
      541 ctcgagcacg ccgaggatgc cgcgcagggc cgccgaatcc gccgcctcgc ggaagagctg
      601 ccgcaccgcc gcggcgtcgg tggccttgcc cacggtggcc acgagctcga cgatggaccc
      661 tgtgcgggtc ggcacccggt aggccttgcc gctgaccggc aggtcgggcc agatgaagag
      721 gagggccttc gccgcccccg acgaggaggg gatcaggttc tccgcggcgg cccaggagtc
      781 gcggcggtcg gccatgggct ggtcggtgag ggactggctg ttcgtgtagg agtgcacggt
      841 cgagaagagg ccgctctcga tcccgaggtt ctcgcgcaga atcttgacca ccggggccag
      901 ggcgttggtc gtgcagctcc ccatgctcac gatgtggtgc ttcttcgggt cgtaggaatc
      961 gaggttgatg ccgggcagga ggaccgcgtc gcagtccgcg agcgacttgg gtccggcgct
     1021 cacgaggacc tttctcgcgc ccttggcgag gtgggcctgg gcgttctcgc gccgcgtccc
     1081 ccgccccgag cagtcgacca ccacatccac cccgagggcc ccccagtcgg ggacggcggc
     1141 cgcggtgtcg acgaagccga tcctccggcc tccgaccctg atcgctccat cctcgaaggc
     1201 ggcctcctcg ggccagcggc cgaagttcga gtccacctcg aagagggccg ccagggtcga
     1261 gggatccttg acgtcgccga tgtggctcgg gacgaaaagc ttccgctcca gggcggcgcg
     1321 gagcatcgtc ctcccgatcc ttccaaaacc gtaaatgccg atattgccca tgggaccctc
     1381 cttgctttgc gagccgatga ccaacgtact gagggccggg gccgagggac aagcgcgggg
     1441 tcggcgctcg ggcggatcgg caaaggctta gcgttaagct aaagttgcag cgggctttga
     1501 gccgagctca ccaatcccgg accggcacgc ccaggagcgt gcgcaggtcg acgagctggc
     1561 cgatgtggta ggaattgtgg tcggtcacga ggattgccag ctcgatgggc gtgcgctcga
     1621 tgcccttcct gagcgggaca tcgagcccgc gcccggggtc gaggacgagg tcgtgcgccc
     1681 gcgccaggtc gtggcggaac ttgtcgatgg tcgagagcca ctcggactcg tccctcgggc
     1741 tgtcgagctt gggccagtag ccgtggggat actcgggcga gtgatgcgag ggcttcaggc
     1801 tgtactcgag gatgtcccac tggcagatcc agaggtggta gaccaggccc caggcgctgt
     1861 ggccgaggtg gggtggcctc ctgcccgcga gctcgaaggg gaaaccctcg agggcatcgt
     1921 ccaacgagat gtgggccccg tggccatcga ggtgctgggc cagggacttc ctgagaccgt
     1981 gcggagcatt gtcggccatc gcgtcctcct tgcgacccgc gccgggcctt aggccttgcg
     2041 tatcctcaca tagagcctcg cgggtgcttc cttgaagtac cagaagaaga ccggacagag
     2101 ccagccctcg tggagctccc cgtcgtcgta ctggtaggtg gcgccgagct cgttcgagtg
     2161 caggaggctg agcagcttgg cgtccttgaa gggcctgtcg ctgtagcgga tcctgacccg
     2221 cctcgcgtcg gagccggcca tgagctcgat gatcttcggg atgcccgcga tgagctcgtt
     2281 gtcgctcttg gagaagccgt tgtcgtcgtc gatgaaccat ccctcctcgt cccggtaggc
     2341 cctcacctcc ggcgtgacga agagcccctt gagccgcatg aggtcccgca gacccggcgc
     2401 gcgaagtcgc atctgtcctc cttgtcggat gcctggagta tagagcgccc gaggagggaa
     2461 tgcgactgga atcgggagga cggccatgag gaggggatga tgggcttccc ctcccgccga
     2521 ctatttggtg aaggcgaaac ctatggccac gcttacggat ggcatcacaa tggtgtatga
     2581 atgatattcc gagaggccag actgcttttg tctatagggg cagaacttag gaagataagg
     2641 ttaaaatcga ttcagccctt gcagacagag ataaggtatt ccaatgatgt aaaggttagg
     2701 caaatcagcg cgaagaaacc tcgcaaggtt ttggctcgaa gctccgcgcg gaaggagaga
     2761 aagcatgaac gtcaacattc gtctggaaac cacgggtgat tacctggcgg tcgaggaact
     2821 gacacgggag gcattctgga atctgtacaa tccgggctgc gatgagcact acctgtgtca
     2881 cataatgagg gaacaccggg acttcatcaa ggatctcgac tatgtcgctg aagttgaagg
     2941 caaaatcgta ggctccatca tgtatacaaa gtccctcctt ctgggcgatg accagatgcg
     3001 ggaggagata gtgtccttcg gccccatatg cgtccatccc gagtaccaga ggagggggat
     3061 aggcacggca ctgatcgaga agacgagaag gctggtcgag aagagcgaca tcccggcgat
     3121 cgcgatctac ggcgatcccc ataactactg caaacatggg ttcaggaatg gcatcgacta
     3181 tggggtgagc agcatggaag gggagcaccc actgggattg cttgtcctgg agctcaggcc
     3241 tggattcttt ggaccgaggg cttggaggct caagcagagc gatgtcttcc agtttgagca
     3301 tgaagaggcc gtggagtttg acaagggctt caatgaaaag gagaagcggg tccagtacag
     3361 ccaggagata ttccagatgc agatcagatc attcctgagg gcgaagccgg cg
//
LOCUS       DASSQV010000039         6060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_261516, whole genome shotgun sequence.
ACCESSION   DASSQV010000039 DASSQV010000000
VERSION     DASSQV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261516"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFL04_00705"
     CDS             complement(<1..325)
                     /locus_tag="VFL04_00705"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HET7838246.1"
                     /translation="MKAFMDEDFLLNSDPARRLYHESAEGKPIIDFHSHLPPKAMAED
                     SRYPSLTEVWLGGDHYKWRAMRANGVPERLITGEAKAEEKFLAWAGTLPRLIGNPLYH
                     WTHLEL"
     gene            471..923
                     /locus_tag="VFL04_00710"
     CDS             471..923
                     /locus_tag="VFL04_00710"
                     /inference="COORDINATES: protein motif:HMM:NF016000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET7838247.1"
                     /translation="MICDVHENWKLYAQPGSRLGRALAWLGAGKAEGLAPGRYEIEGS
                     DIFALVQEYESKPLEACRWEAHRRYIDIQWISRGQEYIDWAALVDCRLLGYQEEKDFQ
                     DCSTEEGLAVAMKQGRFLVLFPEDAHRPGRARPSPQAVRKIVLKVRVD"
     gene            984..2528
                     /locus_tag="VFL04_00715"
     CDS             984..2528
                     /locus_tag="VFL04_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7838248.1"
                     /translation="MAHFGIGIRGKILGFFGMTLAVVIAVEVLAQGAAVRAGLEFEGR
                     LERYHSIQGLRAAFGDFRGLAEQYMRERLPEQEAELEASLIAISELADRIAVLGDESQ
                     DAYFEQRAARRGIEAWAPLVREAIQKRQENRADAYQSYLKADRIAAYVDGYLGKLLSL
                     TMANGSSRYRDIAARSAENRRLALAAIIGAGALAIGFGALFASSISAPVRRLAEAADR
                     MASGDLELEPIAVDTKDEVAVLARGFNAMSANIRALIEGIKEKAELERLLHEESLSRV
                     TMGKALREAQFTNLQDHIRPHFLFNALNTIARSALFEEATETERLARSLAKLMRYSLS
                     EGGSFVTIGEELATLREYLSFQGIRFGSRLAWEVRSDPRVESVLIPRFTLQPIVENAV
                     RHGIEPKEEGGRVIASARLRGGRVRLLVADSGMGMDPALLRRIRESATHEASFGEASF
                     AEASGAGSAQGPSARVGGGAGIGMANLQTRLAFRYPEGVRIAIASRRGRGTIVRITLP
                     GEGDAA"
     gene            2518..3846
                     /locus_tag="VFL04_00720"
     CDS             2518..3846
                     /locus_tag="VFL04_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7838249.1"
                     /translation="MPHRVLIADDEELERRALRRILSGPGLPELQILEAENGREAIEL
                     ALEAGLDAAFLDIRMPGIDGIEAARVLRERFPELPLVFLTAYDSFDYARTALRLRVED
                     FLLKPASEEEVRTALLRALAAASGAHPAPRPLEALLESALGFLGEELRAELGAERVPA
                     EKLERYLRLEGRSGEVRAVAAMRLALHERGQGIAARGGRRKADLDAVAVLFEKAFGST
                     GMLALAGAGQDRVLGVLAGEPGEAFSEASLHARLEGFAEAARAELGLPVLVGVALRDG
                     PLPSPASAELGASALRVAALASPARPVIVCSLRDGGQPRRAAEPESPGSGRRTALRAL
                     ELLEARHAEELSLEVVAAEMGVSASHLSRLLGRVAGLGFADCLARFRVERAKYFLSSS
                     SVSVKEVASLVGFRDPAYFARVFRRFEAMSPAEYRAGASPTQTLREEESE"
     gene            3843..4835
                     /locus_tag="VFL04_00725"
     CDS             3843..4835
                     /locus_tag="VFL04_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HET7838250.1"
                     /translation="MNLKALFAWPLLFCLVLAAAAPGSLAASGPAISLRLAELHPADH
                     PTAQADYEFARLVDERSRGRIRIAVYTDSALGQEISVLEQVQFGAIDIARVSLSAVAS
                     YVPRLNALQMPYLYRDADHMWRVLNGEVGRELLASVRDAGFVGLGWFEAGSRNFYNSR
                     RSVTSPADLKGLRIRVQESQLMSDIVTALGARAVQQPFGEVYSALETGAIDGAENNMP
                     TYFSSQHYKVARYYTLTEHARIPEIVVGSALGLASLSPADLELIRKAAMDAVDFQRKA
                     WDGYEKLAAERVRSAGVVIVSKPDLGPWKALARRAYELQSPEIRALVERIRAVK"
     gene            5025..6002
                     /locus_tag="VFL04_00730"
     CDS             5025..6002
                     /locus_tag="VFL04_00730"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HET7838251.1"
                     /translation="MKLTRIALIALMLTAFVFAVSAQTVLKLAEIHPQGYPTELADEE
                     FAKLVNKYTNGQFKIDIFPGGQISGDEKVVIEQVKINAVSIARVSSGALGSFNAQLNV
                     FSLPYLFDSADHMWAFLLSPAGMKMLDDLAPSGFVGLCWYDGGARSFYANYPLNSIND
                     LKGKKYRVMPNNLLVKMVEAMGASAVPMAAGQVFSAIQTGVIEGAENNAPSLVSFNHS
                     QVAKYYLLDEHLRLPEVLFMNKGVWDKLTKDQQTAFRKAAAETVAFQRKAWDAYVIEA
                     TKKIVAEGEIIVPVPDKTPFKNAVKQLFAEESPKYAETIKAIDAVRPKK"
BASE COUNT          966 a         2163 c         2009 g          922 t
ORIGIN      
        1 tgagctcgag gtgggtccag tggtagaggg ggttgccgat caggcggggc agggtgcccg
       61 cccaggcgag gaacttctcc tcggccttgg cctcgcctgt gatgaggcgc tcgggcacgc
      121 cattcgccct cattgccctc cacttgtagt ggtctccgcc gagccagacc tcggtcaggc
      181 tggggtatcg ggaatcctcg gccatggcct tgggcgggag gtgggagtgg aagtcgatga
      241 tgggcttgcc ttcggcgctc tcgtgataga ggcgtctcgc cggatcggaa ttgaggagga
      301 agtcctcgtc catgaacgct ttcatagggt ctccaagcta ggcggattgg gatttccggg
      361 ctattgtagc ccgagccgcc gatatatgga acaatattcc atatatcggt gaggcctgtc
      421 ggtcgcccgc atgatactcc ccgaggcggc ccaggtgaag gagccaggct atgatctgcg
      481 atgtccacga gaactggaaa ctctatgccc agccgggcag ccgccttggc cgggccctgg
      541 cctggctcgg tgcgggcaag gccgagggcc tggcccccgg gcgctacgag atcgagggca
      601 gcgacatctt cgccctcgtc caggagtacg agtccaagcc gctcgaggcc tgccgctggg
      661 aggcccaccg caggtacatc gacatccagt ggatctccag gggccaggag tacatcgact
      721 gggcggccct cgttgactgc cgcctcctcg gctaccagga ggagaaggat ttccaggact
      781 gctccaccga ggagggcctc gccgtcgcca tgaagcaggg gcgcttcctc gtcctcttcc
      841 cggaggacgc ccacagaccc ggccgggccc gtccctcgcc ccaggcggtg cgcaagatcg
      901 tcctcaaggt gagggtggat tgagggccca tgcttgatgc cgggctattg accatgcggc
      961 gccgcgccgg ggatcatcgg tcaatggccc atttcggcat cggcatccgc ggcaagatcc
     1021 tcggcttctt cggcatgacc ctcgccgtgg tcatcgccgt cgaggtcctg gcccagggcg
     1081 cggcggtgag ggccggcctc gagttcgagg gcaggctcga gcgctaccac tcgatccagg
     1141 gcctgcgcgc cgccttcggc gacttccgcg gcctcgccga gcagtacatg agggagaggc
     1201 tccccgagca ggaggccgag ctcgaggcct cgctcatcgc catctccgag ctcgccgacc
     1261 ggatcgcagt cctcggcgac gagtcccagg acgcgtattt cgagcagagg gcggcgaggc
     1321 gcgggatcga ggcctgggcc cccctggtcc gcgaggccat ccagaagcgc caggagaaca
     1381 gggccgacgc ctaccagagc tacctcaagg ccgaccgcat cgccgcctac gtcgacggct
     1441 accttggcaa gctcctttcg ctgacgatgg ccaacggctc ctcgcgctac cgcgacatcg
     1501 ccgcgaggag cgccgagaac cgccgcctcg ccctcgccgc gatcatcggc gccggggccc
     1561 tcgccatcgg cttcggggcc ctcttcgcga gctcgatctc ggcccccgtg aggcgcctcg
     1621 ccgaggccgc cgaccgcatg gcatcgggcg acctcgagct ggagccgata gccgtcgaca
     1681 ccaaggacga ggtggccgtc ctggcccggg gtttcaacgc gatgagcgcg aacatcaggg
     1741 ccctcatcga gggcatcaag gagaaggccg agctcgagcg cctcctccac gaggagagcc
     1801 tctcgcgggt gaccatgggc aaggccctgc gcgaggccca gttcacgaac ctccaggacc
     1861 acatcaggcc ccacttcctc ttcaacgccc tcaacaccat cgcaaggagc gccctcttcg
     1921 aggaggccac cgagaccgag cgcctcgcca ggagcctggc caagctcatg cgctattccc
     1981 tatctgaggg tggctccttc gtcacgatcg gcgaggagct cgctaccctg agggagtacc
     2041 tctccttcca gggcatcagg ttcggctccc gtctggcctg ggaggtccgc tccgaccccc
     2101 gggtcgagtc cgtcctcata ccgcgcttca ccctccagcc cattgtggag aacgccgtgc
     2161 gccacggcat cgagccgaag gaggagggcg gaagggtcat cgcctcggcc cgtctgcggg
     2221 gaggccgcgt gaggctcctc gtcgccgatt cgggcatggg catggacccc gcactcctca
     2281 ggcggatcag ggagagcgcc acccacgagg cctctttcgg cgaggcctcc ttcgccgagg
     2341 catcgggggc ggggtcggcc caaggcccct cggccagggt cggcggcggg gcggggatcg
     2401 ggatggccaa cctccagacg aggctggcct tccgctatcc cgagggcgtc aggatcgcga
     2461 tcgcctcgag gcgggggcgg gggaccatcg tccgcatcac cctgccggga gagggcgatg
     2521 ccgcataggg tcctcatcgc cgacgacgag gagctcgagc gtcgcgcgct caggcgcatc
     2581 ctctcgggcc ccggcctgcc cgagctccag atcctcgagg ccgagaacgg gcgggaggcc
     2641 atcgagctcg ccctcgaggc cggtctcgac gcggccttcc tcgacatcag gatgcccggc
     2701 atcgacggca tcgaggcggc ccgggtcctc agggagcgct tccccgagct tcccctcgtc
     2761 ttcctcacgg catacgacag cttcgactac gccaggacgg ccctgaggct cagggtcgag
     2821 gatttcctcc tcaagcccgc ctctgaggag gaggtgagga cggccctgct gcgcgccttg
     2881 gccgccgcct ccggggccca tccggccccg cggccgctcg aggccctcct cgagagcgcc
     2941 ctcggcttcc tgggcgagga gctcagggcc gagctcgggg ccgagagggt gccggccgag
     3001 aagctcgagc gctatctcag gctcgagggg aggtcggggg aggtgagggc cgtcgctgcc
     3061 atgcggctcg ccctgcacga gcgcggccag ggcatcgcgg cgaggggcgg caggcggaag
     3121 gccgacctgg acgcggtcgc ggtcctcttc gagaaggcct tcggctccac ggggatgctc
     3181 gcccttgccg gagcagggca ggatcgcgtc ctcggcgtcc ttgccggaga accgggcgag
     3241 gcattctcag aggcctccct tcacgcccgg ctcgagggct tcgccgaggc ggcaagggcc
     3301 gagctcggcc tgcccgtcct cgtgggcgtg gcgctgcgcg atggccccct ccccagcccc
     3361 gccagcgccg agctcggagc ctcggccctg agggtcgccg ccctggcctc gccggcgagg
     3421 cccgtcatag tctgctccct gcgggacggg ggccagccga ggagggcagc cgagcccgag
     3481 agcccaggtt cgggccggag gactgccctc cgcgccctcg agctccttga ggccaggcac
     3541 gccgaggagc tttcgctcga ggtcgtggcc gccgagatgg gcgtctcggc ctcccacctc
     3601 tcccgcctcc tcgggagggt agccggcctc ggcttcgccg actgcctcgc ccgcttccgc
     3661 gtcgagcggg cgaagtattt cctctcgtcg agctcggtct cggtgaagga ggtcgcgagc
     3721 ctcgtgggct tccgcgatcc cgcgtacttc gcgcgggtgt tccggaggtt cgaggccatg
     3781 agcccggccg agtacagggc gggggcatcc ccgacccaga ccctgcgcga ggaggagtcg
     3841 gaatgaacct gaaggccctg ttcgcgtggc ccctgctttt ttgtcttgtt ctcgccgcgg
     3901 ccgcgcccgg atccctcgcg gcctcggggc cggcgatatc cctccgcctc gccgagctcc
     3961 acccggccga ccatcccacc gcccaggccg actacgagtt cgcgaggctc gtcgacgagc
     4021 gctcgcgggg caggatacgc atcgcggtct acaccgactc ggccctcggc caggagatct
     4081 ccgtccttga gcaggtccag ttcggggcca tcgacatcgc gagggtctcc ctgtccgcgg
     4141 tcgcctccta cgtccccagg ctcaacgccc tccagatgcc ctacctctac cgcgacgccg
     4201 accacatgtg gcgggtcctg aacggtgagg tcggcaggga gctcctcgcg agcgtgcggg
     4261 atgcgggctt cgtgggcctc ggctggttcg aggccggctc gaggaacttc tacaactccc
     4321 ggcggagcgt cacgagcccc gccgacctca agggcctcag gatccgcgtc caggagagcc
     4381 agctcatgtc cgacatcgtc acggccctcg gggccagggc cgtccagcag cccttcggcg
     4441 aggtctacag cgccctcgag acgggggcga tcgacggggc cgagaacaac atgcccacct
     4501 acttcagctc gcagcactac aaggtagcgc gctactacac cctcaccgag cacgcgcgga
     4561 tccccgagat cgtggtgggc tcggccctcg gcctggccag cctctccccc gccgacctcg
     4621 agctcatcag gaaggcggcg atggacgccg tcgatttcca gcgcaaggcc tgggacggct
     4681 atgagaagct cgccgccgag cgcgtcagga gcgccggggt ggtgatcgtc agcaagcccg
     4741 acctcgggcc atggaaggcc ctggccaggc gggcctacga gctccagagc cccgagatac
     4801 gcgccctcgt cgagcgcatc cgcgccgtga agtagggcgg ccagagagca tcagaatacc
     4861 ggaaaaagca tgattctcct ccaccgcgct tgcgggatag gccctaggat gccgaaggga
     4921 gagttcgggc cgcacgcgcg gccagagcct ccctgcgcgc acgcgccctc ggggccaggc
     4981 caccgaagga cggcggcgcg cggacaccac aaatggaggt agggatgaaa ctcacgcgca
     5041 tcgctctcat cgcgctcatg ctcaccgcgt tcgtcttcgc ggtcagcgcc cagacggtcc
     5101 tgaagcttgc ggagatccac ccgcagggtt acccgaccga gctcgcggac gaggagttcg
     5161 ccaagctggt caacaagtac acgaacggcc agttcaagat cgacatcttc cccggcggcc
     5221 agatctccgg cgacgagaag gtcgtcatcg agcaggtcaa gatcaacgcg gtcagcatcg
     5281 cccgcgtcag ctccggcgcc ctcggctcct tcaacgccca gctcaacgtc ttcagcctgc
     5341 cctacctctt cgattcggcc gaccacatgt gggccttcct cctctccccc gccggcatga
     5401 agatgctcga cgacctcgcc ccctcgggct tcgtcggcct ctgctggtac gacggcggcg
     5461 cgcgctcctt ctacgccaac tacccgctca acagcatcaa cgacctcaag ggcaagaagt
     5521 accgcgtcat gccgaacaac ctcctcgtga agatggtcga ggcgatgggc gcgagcgccg
     5581 tccccatggc cgccggccag gttttcagcg ccatccagac cggagtcatc gagggcgccg
     5641 agaacaacgc cccctccctc gtctccttca accactccca ggtcgccaag tactacctcc
     5701 tcgatgagca cctccgcctg cccgaggtcc tcttcatgaa caagggcgtc tgggacaagc
     5761 tcacgaagga tcagcagacc gccttccgca aggccgccgc cgagaccgtc gccttccagc
     5821 gcaaggcctg ggacgcctac gtcatcgagg ccacgaagaa gatcgtcgcc gagggcgaga
     5881 tcatcgtccc cgtgccggac aagaccccct tcaagaacgc cgtcaagcag ctcttcgccg
     5941 aggagtcgcc gaagtacgcc gagaccatca aggcgatcga cgccgtccga cccaagaagt
     6001 aagggcatca ccagcgccgc ccggacctgg aggcttcgag ccccggggcc gggcggccct
//
LOCUS       DASSQV010000040         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_138646, whole genome shotgun sequence.
ACCESSION   DASSQV010000040 DASSQV010000000
VERSION     DASSQV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138646"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="VFL04_00735"
     CDS             complement(<1..1579)
                     /locus_tag="VFL04_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7838252.1"
                     /translation="MIAVDEGPPLYNSKITNSYLKLIKSRYSYVNVTELLSYAGMEPY
                     QVEDDGHWFTQAQVDRFHERIVALTGNHAISREAGRYNASPEGLGTIPRYVFGLAGPA
                     KAFEVIHKLAGNLTRSTKYQYRRLGSTHIELTVIPNAEVREKPYQCENRMGYFDAIVA
                     GFNYRLPRIEHSECVFKGSAVCRYDITWRESAAATWKRIRGAFAGTAILGLAAIAFIF
                     PLRVLLDSAAISAISLLVLSFMAEYHERRELSASIDNLHLSTERFLENGDRNYNHALM
                     INEIGRIISKYRQTDSLLSQVIEIMRRRLDYDRGLILLANEDKSLLEFRSGYGYEPSI
                     IDEIKQARFHLDHADSRGAFVLCYRQRRPYLVNDVEEIEGELSPRSLDFLRKLGSKSF
                     ICCPILYEDECLGVLAVDNLQSKRPLLESDINLLMGIAPEIGISLYNAMLTDEREQQF
                     RSILRTLAASIDARDSLTAGHSEKVTEYAMAICGELSLGRETTDVIRVASLLHDYGKI
                     AIKDSILKKTGPLSGKER"
     gene            complement(1563..>2582)
                     /locus_tag="VFL04_00740"
     CDS             complement(1563..>2582)
                     /locus_tag="VFL04_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838253.1"
                     /translation="INGNVEQDELWRLFFESGFIYPSKYLSIQEHKEDFRLTYEKLYL
                     DSPSIGRHFVVQDRGRLLGHMSMLRYYSNTWIIHHHTASRDGRGMAGVGVLDEIGRFV
                     NEFHSHPSTHMDYLICYYRKENRFPCRVFGNVVRDIDDPKGSSVDAFAYLHLETGVAE
                     DPYPFQLFPARAEEFARLRRWYEEVSGGLMLDALDLGEDRLDDSALELEYARLGFKKE
                     RHIFCLKQDGRLMAILVLSLSELGLNLSNLTSCVHAIVIEPERLAAGALFSGVRSLLH
                     QYSCEEIPVLVHPAAYMDARELKYEKTYLLWVLNLDRTDGYLDSLRHTFRRVSDDGNG
                     HDSCG"
BASE COUNT          465 a          737 c          881 g          499 t
ORIGIN      
        1 cgcgctcctt gccgcttaga gggccggtct tcttgaggat cgagtccttg atggcgatct
       61 tgccgtagtc gtgcagcagc gaggcgaccc tgatcacgtc ggtggtctcc ctgccgaggg
      121 agagctcccc gcagatcgcc atcgcgtact cagtgacctt ctcggaatgg cccgcggtaa
      181 ggctgtcgcg ggcgtcgatg ctcgccgcga gggtcctgag gatcgagcgg aactgctgtt
      241 cgcgctcgtc ggtcagcatc gcgttgtaga ggctgatccc gatctcgggg gcgatgccca
      301 tgaggaggtt gatgtcgctc tcgaggaggg gccgcttgct ctgcaggttg tccaccgcga
      361 ggaccccgag gcactcgtcc tcatagagga tggggcagca gatgaaggac ttcgagccca
      421 gcttgcgcag gaagtccagg ctccgcggcg agagctcgcc ctcgatctcc tcgacgtcgt
      481 tgacgagata gggccgccgc tgacggtagc agaggacaaa ggccccgcgg gagtcggcat
      541 ggtccagatg gaaacgcgcc tgcttgatct cgtcgatgat ggagggctcg tatccgtagc
      601 cagagcggaa ctcgaggagg ctcttgtcct cgttcgctag aagaatgagg cccctgtcgt
      661 agtcgaggcg ccggcgcatg atctcgatga cctgggacag gaggctgtcg gtctgacggt
      721 acttgctgat gatccgcccg atctcgttga tcatgagggc atggttgtag ttgcggtcgc
      781 cgttttcgag gaaacgctcg gtggaaagat gcagattgtc gatggaggcg ctgagctccc
      841 gcctctcgtg gtactcggcc atgaaggaga ggacgaggag gctgatcgcc gatatggcgg
      901 cgctgtccag gaggacccgc aggggaaaga tgaaggcgat cgccgcgagg cccaggatcg
      961 ccgtccccgc gaaggcgccc cggatccgct tccaggtggc cgccgccgat tccctccagg
     1021 tgatgtcgta cctgcagacg gcgctgccct tgaacacgca ctcgctgtgc tcgatccggg
     1081 gtagccggta gttgaagccc gcgacgatgg cgtcgaagta gcccatccgg ttctcgcact
     1141 ggtagggctt ctcacggact tcggcgttcg gtatcacggt gagttcgatg tgggtggatc
     1201 cgagcctccg gtactggtat tttgtcgagc gcgtgaggtt gccggcgagc ttgtggatca
     1261 cctcgaaggc cttcgccgga ccggccagcc cgaagacgta gcgcgggatg gtcccaaggc
     1321 cctcgggcga ggcgttgtag cggccggcct cgcgcgatat cgcgtggttg cccgtgaggg
     1381 ccacgatgcg ttcgtggaaa cggtccacct gggcctgggt gaaccaatgg ccgtcgtcct
     1441 cgacctgata gggctccatg ccggcatagg acaggagctc cgtgacgttg acgtaggagt
     1501 accggctttt tatcagcttg aggtaggagt tggtgatctt gctgttgtag agcggcggcc
     1561 cctcatccac agctatcatg cccatttccg tcatcggaaa ccctcctgaa ggtatgccgc
     1621 agtgagtcca ggtagccgtc ggtgcgatcc aggttcaaca cccaaaggag gtaggtcttt
     1681 tcgtatttca gctcgcgcgc gtccatgtag gcggcgggat ggacgaggac ggggatctcc
     1741 tcacaggaat actgatggag cagggaccga acccccgaga acagggcccc ggccgccagc
     1801 ctctccggct ctatgacgat cgcgtggacg cagctggtca ggttggagag gttgagcccg
     1861 agctccgaga ggctgaggac gaggatggcc atcaggcgcc cgtcctgttt gaggcagaag
     1921 atgtgccgtt ccttcttgaa gcccagcctg gcgtattcca gctcgagcgc cgaatcgtca
     1981 aggcggtcct cgcccaggtc cagtgcgtcg agcatgaggc cccccgagac ctcctcatac
     2041 cagcgccgga gccgggcgaa ctcctcggcg cgggcgggga agagctggaa tggataggga
     2101 tcctccgcca ccccggtctc gagatggaga taggcgaagg cgtccacgga ggagcccttc
     2161 gggtcgtcga tgtcgcgcac gacgttgccg aagaccctgc aggggaagcg gttctccttg
     2221 cggtaatagc agatgaggta gtccatgtgg gtcgaaggat gagaatggaa ctcattgacg
     2281 aagcggccga tctcgtcgag gacgccgacc ccggccatcc cgcggccgtc acgcgaagcc
     2341 gtgtggtggt ggatgatcca ggtgttcgag tagtaccgga gcatggacat gtggcccagg
     2401 agcctgcccc ggtcctgcac gacgaaatgc ctgccgatag aagggctgtc gagatagagc
     2461 ttctcatagg tgagcctgaa atcttccttg tgctcctgga tcgagaggta cttggagggg
     2521 taaatgaagc ccgactcgaa gaaaagccgc cagagctcat cctgttccac gttgccgttt
     2581 at
//
LOCUS       DASSQV010000041         4300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_510972, whole genome shotgun sequence.
ACCESSION   DASSQV010000041 DASSQV010000000
VERSION     DASSQV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510972"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFL04_00745"
     CDS             <1..258
                     /locus_tag="VFL04_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HET7838254.1"
                     /translation="GEGVTIFFSMGKRQRIFPRTLIDLILEKGGVALEDIGEVRAFDN
                     YSFADIAPAKASALVASLDGTELRGRKLSVSLAKRRDESAT"
     gene            complement(287..1432)
                     /locus_tag="VFL04_00750"
     CDS             complement(287..1432)
                     /locus_tag="VFL04_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838255.1"
                     /translation="MLYPVYLALAIAFLFYLAIPIAGAFKARGQWRRFRSRVLELSLY
                     PQLRYHDLALASSRRGGGPPSSRLPVGRFRLYGKVEAMVGKDRIWVRGKSVSTLIDFS
                     RTPLFVLEAGEPRAGAIERLAWRNVSSLGEGTKILVGGSLVLEEGQPVFADDPDVPLL
                     VVSYDREDEGLVADLIAAGRPENEYWNSATRISFALGMTLSSILLVLLIARDLFPSVR
                     ALAFLAAMTPVLGLSPPGLGFFILYRHLWRRALLFRMERDLVALPMRYFRPGRVDPAA
                     GEARAALPGGGSYVLKRLPEGGSKHTAASVRGIRASRAGADWVLFAPEGSDDPAAETV
                     IVAGDPVALARAADRKALIIVLAAALAFAAAVVVNYVIAFIVWRLMP"
     gene            1474..2127
                     /locus_tag="VFL04_00755"
     CDS             1474..2127
                     /locus_tag="VFL04_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7838256.1"
                     /translation="MVERLKVGPIGENAYVILGSRDCILVDPGDEAQRILAFLDSRDL
                     VPSHIVLTHGHLDHTAAIPELLAVWAERGFGPLLAAHSLDADYFGESGEEKNRDLFAA
                     IRAIGYFRSYWKAMKGIDLLLEDGATLPVGGFRVIHTPGHTRGSICLYGQAERILISG
                     DTLFESGVGRTDGPDASQAELEKSLARLALLPPETVVLPGHGEATTIGRELGADGSD"
     gene            2207..3277
                     /locus_tag="VFL04_00760"
     CDS             2207..3277
                     /locus_tag="VFL04_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET7838257.1"
                     /translation="METTSIQAPRGARLFHLTLAALAFASLALGCAPRPAAEVSGFEQ
                     VLGTACNLRLAKGGSEKLLKAGFERLHALENEISVNVDGSEIEAVNAAAGQHPVKVGQ
                     DSMAILSRDLEYSRLSDGAFDPSVGPLVQLWGIGGIRAKGSESELPPPPQIAAALAKV
                     GWKDVVLNKEAGTAFLRRPGMALDLGSGSKGYAADLLVDLFRKGGVTSALIDLGGNIY
                     VMGSKPDGKPWRVGLQNPDATRGTYVGVASLVDKTMVTSGVYERYFFHNGKRYHHILD
                     TRTGYPVDNGLTSVTIITDRSFDADGLTTTLFAMGRQKGMELAKQRGVDVIMIDDQRK
                     VYLSPGVSKYFELTDSSYTYAE"
     gene            complement(3294..4193)
                     /locus_tag="VFL04_00765"
     CDS             complement(3294..4193)
                     /locus_tag="VFL04_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7838258.1"
                     /translation="MDGILGWGLNAVRSIQAFASPMLTSLMLGLSWFGNLWLILLAVP
                     AVYWCVDRRRGARLGILMFISGFVNLWLKLVFAQPRPYDLDQSVALARESTFGLPSGH
                     AQNSLVFYSGIEPLIPRPWGALAAIALPVLIGFSRVYLGVHFPSDVLVGWAIGLIILL
                     CDRLFGDWVERLFALLRERLQLALVAAIALAMNAIDKSEAALTGAFFGFAAGLVYMPR
                     LAPIETGGSAGKRTLRYLFGIGVALGLYALLNLASKGLEPMSPSLVRFVSSAVLGLWL
                     SLGAPLAFIRIGLASREKDEAEE"
BASE COUNT          847 a         1418 c         1371 g          664 t
ORIGIN      
        1 ggcgagggag tcacgatctt cttctcgatg gggaaacggc agcgcatctt cccgcgcacc
       61 ctcatcgacc tgatcctcga aaagggtggg gtcgccctcg aggacatcgg agaagtcagg
      121 gccttcgaca actattcctt cgcggacatc gcgccggcca aggcctcggc cctcgtcgca
      181 tccctcgacg ggaccgagct caggggccgc aagctctccg tgagccttgc gaagcggcgg
      241 gacgaatccg cgacctgatg cccgtgaggt ctagccctcg agcttcctag ggcatcagcc
      301 tccagacgat gaaggcgatc acatagttga cgaccacggc cgcggcgaag gcgagggccg
      361 cggcaaggac gatgatgagg gccttcctgt ccgcggcgcg ggccaaggca acggggtcgc
      421 cggcgacgat gacggtctcg gcggccggat cgtccgagcc ttcgggagcg aagagcaccc
      481 aatcggcccc ggcccgcgac gccctgatgc cccggaccga ggccgctgtg tgcttcgagc
      541 ctccttcggg gagtcgtttc aggacatagc tcccgccccc tggaagggct gccctcgcct
      601 cgcccgcggc cggatcgacc cgccccgggc ggaagtaccg catgggcagg gcgacaaggt
      661 cgcgctccat gcggaagagg agggcccgcc gccagaggtg gcggtagagt atgaagaagc
      721 cgagcccggg aggcgacagg cccaagacgg gggtcattgc cgccaggaaa gccagggccc
      781 tgaccgaggg gaagaggtcc cgggcgatca ggagaaccag gagaatgctc gaaagggtca
      841 tgcccaaggc gaaggaaatc ctggtcgctg agttccagta ctcgttttcg ggtcttcccg
      901 ccgcgatgag gtcggcgacc aggccctcat cctcccgatc gtacgagacg acgagcaggg
      961 gcacatcggg atcgtcggcg aaaaccggct ggccctcctc caggaccagg cttcccccga
     1021 ccaggatctt cgtcccctct cccagcgagg acacgttcct ccaggccagg cgctcgatcg
     1081 cacccgcacg gggctcgccc gcctccagga cgaacagggg tgtgcgggag aaatcgatga
     1141 gggtcgatac ggattttccc ctgacccaga tcctgtcctt ccccaccatg gcctcaacct
     1201 tgccgtagag cctgaaacgt ccgacgggca gccttgagga gggagggccg cccccgcgcc
     1261 gggatgaggc cagggccagg tcatggtatc gcagctgggg atagaggctg agctcaagca
     1321 cccggctcct gaagcggcgc cactgccccc tggccttgaa ggcgccggcg atcgggatgg
     1381 cgagataaaa caggaaggct atcgccagag ccaggtatac cggatacagc acggcttgca
     1441 gctcctggga tcgaatcggt actctaggct atcatggttg aacgcctgaa ggtaggaccg
     1501 ataggggaga acgcctacgt gatactcggc agccgggact gcatcctggt cgatccgggt
     1561 gacgaggccc agcgcatcct cgcgttcctc gactcgcggg acctggtccc ctcgcacata
     1621 gtcctcaccc acggccatct cgaccacacc gccgcgattc ccgaactcct cgccgtctgg
     1681 gccgagcggg gcttcggtcc tctcctcgcg gcgcattccc tcgacgccga ttacttcggc
     1741 gaaagcggag aagaaaaaaa ccgcgacctc ttcgcggcga tccgcgcgat tggctatttc
     1801 cggtcctact ggaaagccat gaagggcatc gatctcctgc tcgaggacgg cgcgaccctg
     1861 ccggtcggcg gcttcagggt cattcacacc ccgggccata cccggggctc gatctgcctc
     1921 tacggtcagg ccgagaggat cctgatttcc ggcgacaccc tgttcgaatc cggcgtcggc
     1981 aggacagacg gccccgacgc cagccaagcc gaactcgaga agagcctcgc ccgtctcgcc
     2041 ctcctgcccc ccgagacggt cgtcctgccc gggcacggcg aggccacgac gatcggcagg
     2101 gagctcggcg cagacgggag tgactgaagg cgaagctcct cgctgccgat gccgaggact
     2161 tcgattaatt gatcaaaacc ctcggttgct ccatactacg ccgaccatgg aaaccacaag
     2221 catccaggct ccccgcggcg cccgtctttt tcatctcacc ctcgctgcac tcgccttcgc
     2281 cagcctggcc cttggctgcg ctccccgccc tgcggccgag gtctccggtt tcgagcaggt
     2341 cctcgggacc gcctgcaacc tcagactcgc gaaggggggc tcggagaagc tgctcaaggc
     2401 gggcttcgaa cgtctccacg ccctcgagaa cgagataagc gtcaatgtcg atggctcaga
     2461 gatcgaggcc gtaaacgccg cagccggcca acaccctgtc aaggtgggac aggacagcat
     2521 ggccatcctc agccgcgacc tcgagtattc taggctctcg gacggagcct ttgatccgag
     2581 cgtgggcccc ctcgtccagc tttgggggat aggaggcata cgggccaagg gctcggaatc
     2641 ggaactccct ccgcccccgc agatagcggc cgccctcgcg aaggtcggat ggaaggacgt
     2701 cgtcctcaac aaggaagccg gcacggcctt tctgaggcgt cccggcatgg ccctcgatct
     2761 cggctcgggg tccaagggct acgccgccga tctcctcgtc gacctcttcc gaaagggcgg
     2821 cgtaacgagc gccctcatcg atctcggcgg caacatctac gtcatgggtt cgaagccgga
     2881 cggaaagccg tggagggtcg gcctccagaa tcccgacgcc acccgcggaa cctatgtcgg
     2941 cgtcgcgagc ctagtggaca agacgatggt cacctcgggg gtctatgagc gctacttctt
     3001 ccacaacggg aagcgctatc atcacatact cgacacgagg accggctacc ctgtggacaa
     3061 cggcctcact tcggtcacca tcatcaccga caggtccttt gatgccgacg gcctcaccac
     3121 aacccttttc gcgatgggca ggcagaaggg aatggagctc gccaagcagc gtggggtaga
     3181 tgtcatcatg atcgacgacc agaggaaggt gtacctgagc ccgggcgtct ccaagtactt
     3241 cgagctgacc gattcctcct acacctacgc ggaataggga gcctcccaag ccctcactcc
     3301 tcggcctcat ccttctccct cgaggccaag ccgatcctga tgaaggcgag gggagcgccc
     3361 agggagagcc aaagccccag gacagcgctg ctcacgaagc gaacgaggga ggggctcatc
     3421 ggctcgaggc cctttgaggc gaggttcaga agggcgtaga ggccgagggc cacgccgatc
     3481 ccgaagagat agcggagcgt acgcttccct gccgaccctc cggtctcgat gggcgcaagc
     3541 ctcggcatgt agacgaggcc cgcggcgaat ccgaagaatg cgcccgtgag ggcggcctcc
     3601 gatttgtcta tcgcgttcat cgccaaggca atggcggcga cgagggccaa ttggaggcgc
     3661 tccctgagca aggcgaacag cctctccacc cagtcgccga acaggcggtc acagagcagg
     3721 atgatgaggc cgatcgccca gccgaccagg acgtcgcttg gaaaatgtac cccgaggtag
     3781 acccggctga atccgatcag cacaggcaag gcgatcgcgg caagggcgcc ccagggcctt
     3841 gggatgagcg gctcgatgcc gctgtagaag acgagggaat tctgcgcgtg gcccgaaggg
     3901 aggccgaatg tcgactccct tgcgagggcg accgactggt cgagatcata gggcctgggt
     3961 tgcgcgaaca cgagcttgag ccagagattc acgaagcccg agatgaacat gaggatgcca
     4021 aggcgggcgc ctcggcgcct gtcgacgcac caatacaccg cgggcacggc aagcagaatg
     4081 agccaaagat tgccgaacca gctcaggccc agcatcaggg aggtgagcat cgggctggcg
     4141 aaggcctgga tcgatcgaac cgcgttgagc ccccagccga gaattccatc cattatgagc
     4201 ccccttcgtg atagagtacg cgcgggagct ccgatgccgc aaggcttgac ggcgcgtccc
     4261 tcccgtagta gaaacgtcca ttccgccggc ctggtgaaat
//
LOCUS       DASSQV010000042         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1754731, whole genome shotgun sequence.
ACCESSION   DASSQV010000042 DASSQV010000000
VERSION     DASSQV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754731"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..557
                     /locus_tag="VFL04_00770"
     CDS             174..557
                     /locus_tag="VFL04_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838259.1"
                     /translation="MRITTRFAPFAATLLLFLLLSCRGSGEGSVKLDPTPPISRGPGW
                     AIVKEAYARLKERPSAASADKAHLRRGSVVELEGRELGLPGKDEDRGFWYALKAEGIE
                     GFMRETELDVFSTRELAERAAVSYR"
     gene            complement(565..1908)
                     /locus_tag="VFL04_00775"
     CDS             complement(565..1908)
                     /locus_tag="VFL04_00775"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET7838260.1"
                     /translation="MDRARFSSSEEIFESLLAFVNVEKGQKTEFKLDRMAALCAALGH
                     PEAAYETIHVAGSKGKGSVSAMIARILEASGSKTGLYTSPHLLRWKERISLAGVEMPE
                     SILIAAAEEVLPLVDGRTGGDFPGGELPTYFELSTLIAFCAFRLAGCEWVVVETGLGG
                     RLDSTNVVPSRASVITPIELEHTEWLGDSIPKIAFEKAGIMKPGRPCYLSRQRPEALE
                     VFRRASADRGSPLNEAPLLARIAEVSVEARGTRAMVDLSAATGLPPGFGLPLELSTPM
                     VGGIQAENMALAVLAAAGSFAGVSPETARAGLAKARLPARFQVLDLQPQVVVDGAHTP
                     DSCRIALASFELLFPGRKALLFACALDKNHAEMARILASGFETVIVTKPGNFKQSDPG
                     AVLESFRRAGARVRLIEDTGEAVRAARSEALASGLPLLVAGSFYLCAEALMILGV"
BASE COUNT          354 a          707 c          760 g          323 t
ORIGIN      
        1 accttctcgt cgtacttcag gtaactcgat gtgcgtatga gatagaccat cctcgtgcct
       61 ccatgcccag aaaaatagcg aggctccggg aagagggcga ggggaaaccg tcacgcacgg
      121 cttcacgtct cgggcccgat cggatagagt caccgcgagg gatggtacca gcaatgcgca
      181 tcacgacccg tttcgccccc ttcgcggcca cactccttct tttcctcctg ctctcctgca
      241 ggggctcggg cgagggctcg gtgaagctcg atcccacgcc gcccatatcg agggggccgg
      301 gatgggccat cgtcaaggag gcctacgcgc ggctcaagga gaggcccagc gccgcctccg
      361 ccgacaaggc ccacctgagg cgggggagcg tcgtcgagct cgagggcagg gagctgggcc
      421 tgccgggcaa ggacgaggac cggggcttct ggtacgcgct caaggccgag ggcatcgagg
      481 gcttcatgcg cgagaccgag ctcgacgtct tctcgaccag ggagctggcc gagcgggcgg
      541 cggtctccta tcggtgaggg gccctcagac cccgaggatc atgagggcct cggcgcagag
      601 gtagaaggag ccagcgacga ggaggggcag gcccgaggcc agggcctcgg aacgggcggc
      661 gcgcaccgcc tccccggtgt cctcgatgag cctgaccctc gcgcccgcgc gtctgaagct
      721 ctccagcacg gccccgggat cgctctgctt aaagttgccc ggcttcgtca cgatcacggt
      781 ctcgaagccg gacgcgagga tcctcgccat ctcggcgtgg ttcttgtcga gggcgcaggc
      841 gaagagcagg gccttcctcc cggggaagag gagctcgaag ctggcgaggg cgatccggca
      901 cgagtcgggg gtatgggccc cgtcgaccac cacctgaggc tggagatcga ggacctggaa
      961 gcgggcgggc agcctggcct tcgcgaggcc ggcccgcgcg gtttcgggac tgactccggc
     1021 gaagctcccc gccgcggcca ggacggcgag ggccatgttc tcggcctgta tgcccccgac
     1081 catcggcgtg ctgagctcga ggggaaggcc gaagcccggc gggagcccgg tggcggcgga
     1141 gaggtcgacc attgccctcg tcccgcgcgc ctcgacggag acctcggcga tcctcgcgag
     1201 gaggggagcc tcgttaaggg ggctgcctcg gtccgcgctg gccctgcgga agacctcgag
     1261 ggcctcgggc ctctgacggc tcaggtagca gggcctcccc ggcttcatga tgcccgcctt
     1321 ctcgaaggcg atcttgggga tgctgtcgcc gagccactcg gtgtgctcaa gctcgatggg
     1381 cgtgatcacg ctggcccggg agggcacgac gttcgtcgag tccagcctcc cgcccaaccc
     1441 ggtctcgacg acgacccatt cgcagcccgc aagcctgaag gcgcagaaag cgatcagggt
     1501 gctgagctcg aaataggtgg ggagctcgcc gccggggaaa tcccctcctg tcctcccatc
     1561 gacgaggggg aggacctcct cggccgcggc gatcaggatg gactcgggca tctccacgcc
     1621 cgcaaggctg atcctctcct tccagcgaag gaggtggggg gaggtgtaga gcccggtctt
     1681 cgagcccgag gcctcgagga tccgcgcgat catggcggag accgagccct tgcccttgga
     1741 ccccgccaca tggatggtct cgtaggcggc ctcgggatgg ccgagggcgg cgcagagcgc
     1801 ggccatccgg tcgagcttga actcggtctt ctggcccttc tccacgttca cgaaggccag
     1861 gaggctttcg aatatctcct cggaggagga gaagcgcgct ctgtccatga cgagggatca
     1921 tggcgaagcc ccgcgagagc gtcaagacga gcccctgccg agccgatatt ggatgctatg
     1981 aagacggaaa gcttcgaggg gcccggcggc atggcccggg ccgcctcgcg cccttgccgc
     2041 acgcgccaga acggccgccg cagggaggtg gccggctcgg ccatcctcgt cgccctcgat
     2101 tccgacggga cggtgttcga ctccatgagc tgcaaacacg agct
//
LOCUS       DASSQV010000043         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_13945, whole genome shotgun sequence.
ACCESSION   DASSQV010000043 DASSQV010000000
VERSION     DASSQV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13945"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VFL04_00780"
     CDS             complement(<1..863)
                     /locus_tag="VFL04_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET7838261.1"
                     /translation="MRGLEHRGLAHAEGPIIIAQSRETAGFGELVAVYGRDGGRRVGR
                     VVDSSDRAVAVQLFSANAGLSLDETRMEYLGRPLELRVGADLLGRVFDGLGQPADGYP
                     EILSSEFRDVNGSPINPAARVYPKDFIQTGVSAIDGLNTLIRGQKLPIFSGNGLPHNR
                     LAAQIVRQARILSPDSGSGAPDRAGGAGDDAFAIVFAGMGIKYDVARFFVDSFERSGV
                     LSRVVMFQSLADSPTIERLITPRCALTAAEYLAYERNMHVLVVLTDMTNYCEALREVS
                     AARGEVPSRKG"
     gene            complement(860..>2593)
                     /locus_tag="VFL04_00785"
     CDS             complement(860..>2593)
                     /locus_tag="VFL04_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET7838262.1"
                     /translation="PVVLAEGLGGAGLYDVAEVGEAGLSGEIIRIDGSGATIQVYEEN
                     TGMRPGEPVASLGRPLSVALGPGLLGTIYDGTQRPLEELKAASGSFIKAGVKGEPLSL
                     GRKWPFVPALRVGEPVGPGTLLGTVQETQLTLHRVMAPPFTRPGLATRVAPAGDYSLR
                     DSIVESDRGEVLRLAHDWPARLARPARRRLAPTEPLLTGLRVIDILFPMSKGGAAAVP
                     GAFGTGKTMTQHALAKYCDADIIVYVGCGERGNEMTEVLTEFPHLVDPRTGRSIMERT
                     ILVANTSNMPVAAREVSIYTGITIAEYYRDMGYDVAVMADSTSRWAEALRELSGRLEE
                     MPAEEGFPAYLPTRLAEFYERGGRVETLGGSTGSVSIIGAVSPPGGDFSEPVTQHTKR
                     FIRCFWGLDKDLAYARHYPAISWIDSYSEYAEELGEWWDREEPGWRPVRLRALDLLKR
                     EQRLEQIVRLIGPDALPDEQRLVLVTADLIKNGFLQQSSFDPVDRYCPPEKQVLLIQA
                     IMEFHRRAELAIKAGAPLPKIVALPIRERLSRLKSEVPNESLEGLRGIEAEIAAVFEE
                     LQRQYRGEVAR"
BASE COUNT          371 a          860 c          968 g          394 t
ORIGIN      
        1 tagcccttgc gggagggcac ctcgccccgg gcggccgaga cctctcgcag ggcctcgcag
       61 tagttggtca tgtccgtgag gacgacgagg acgtgcatgt tgcgctcata ggcgaggtac
      121 tcggccgccg tcagggcgca gcgcggggtg atgaggcgtt cgatggtggg gctgtcggcc
      181 agggactgga acatgacgac ccgggagagg acgccggagc gctcgaagga gtccacgaag
      241 aaacgggcga cgtcgtactt gatgcccatg cccgcgaaga cgatggcgaa ggcatcgtct
      301 ccggcgccgc cggcgcggtc cggggcgccg gagcccgaat ccggggagag gatcctcgcc
      361 tggcggacga tctgggcggc gaggcggttg tgcggcaggc cgttgcccga gaagatcggg
      421 agcttctggc ctcggatgag ggtattgagg ccgtctatcg ccgacacccc ggtctggatg
      481 aagtccttcg gatagacgcg ggccgcgggg ttgatcgggc tgccgttgac gtcccggaac
      541 tcggagctga ggatctcggg atagccgtcg gcgggctggc cgaggccgtc gaatacccgg
      601 ccgaggaggt cggcgccgac cctgagctcg agggggcggc cgaggtactc catcctcgtc
      661 tcatccaggc tcagcccggc gttcgcggag aagagctgga cggcgacggc cctgtccgag
      721 ctgtccacga ccctgccgac ccgacgcccg ccgtcccgac cgtagacagc gacgagctcg
      781 ccgaagccgg ccgtctcccg gctctgcgcg atgatgatgg ggccctcggc gtgggcgagg
      841 ccccggtgct cgagccccct catctggcca cctcccctcg gtactggcgc tgcagctcct
      901 cgaagacggc ggcgatctcg gcctcgatgc cgcggagccc ttccaggctc tcgttcggaa
      961 cctcgctctt gaggcgggac agccgctccc gtatgggcag ggccacgatc ttgggaagcg
     1021 gggcccccgc cttgatggcg agctcggccc tcctgtggaa ttccatgatg gcctggatga
     1081 ggaggacctg cttctcgggc gggcagtagc ggtcgacggg gtcgaaggag ctctgctgga
     1141 ggaagccgtt cttgatgagg tcagccgtga ccaggacgag gcgctgctcg tccggcaggg
     1201 cgtcggggcc gatcaggcgg acgatctgct cgagccgctg ctcgcgcttg agcaggtcga
     1261 gggccctgag ccgcaccggc ctccagccgg gctcctccct gtcccaccac tcccccagct
     1321 cctcggcgta ctcggagtag gagtcgatcc aggagatcgc gggatagtgg cgggcgtagg
     1381 ccaggtcctt gtcgaggccc cagaagcagc ggatgaagcg cttcgtgtgc tgggtcaccg
     1441 gctcggagaa gtcgcccccc ggcggggaga ccgccccgat gatggacacc gagcctgtcg
     1501 agccgcccag ggtctcgacc cggccgccgc gctcgtagaa ctcggcgagg cgggtgggca
     1561 gataggcggg gaagccctcc tcggccggca tctcctcgag gcggcccgag agctcacgga
     1621 gggcctcggc ccagcgcgag gtggagtcgg ccatgacggc gacgtcgtag cccatgtcgc
     1681 ggtagtactc ggcgatggtg atgcccgtgt agatggacac ctcgcgcgcc gcgaccggca
     1741 tgttcgaggt gttcgcgacg aggatcgtcc tctccataat ggagcgcccc gtgcgggggt
     1801 cgacgaggtg ggggaactct gtgaggacct cggtcatctc gttgccccgc tccccgcagc
     1861 cgacgtagac gatgatgtcg gcgtcgcagt atttggcgag ggcgtgctgg gtcatggtct
     1921 tgccggtgcc gaaggctccc ggcaccgccg cggccccgcc cttggacatc gggaagagga
     1981 tgtcgatgac gcgcagcccg gtgaggaggg gctcggtggg ggccagcctt cgcctcgccg
     2041 gcctcgcgag ccgggcgggc cagtcgtggg cgagcctcag gacctcaccc ctgtcgctct
     2101 ccacgatgct gtcgcgcagg ctgtagtcgc cggccggggc gacccgggtc gccaggcccg
     2161 gcctcgtgaa gggcggggcc atgacgcggt ggagggtgag ctgggtctcc tggacggtcc
     2221 caagaagcgt ccctggcccg accggctctc cgaccctcag ggccgggacg aagggccact
     2281 tcctcccgag ggagaggggc tcgcccttga cccccgcctt gatgaaggag ccggaggccg
     2341 ccttcagctc ctcgagcggc ctctgggtcc cgtcgtagat cgtgccgagg aggcccgggc
     2401 ccagggcgac cgacaggggc ctgccgagcg aggccacggg ttcgccgggc ctcatgcccg
     2461 tgttctcctc gtagacctgg atggtggccc ccgagccgtc gatgcggatg atctcgccgg
     2521 agagcccggc ctcgcccacc tcggcgacgt cgtagagccc ggccccgccg aggccctcgg
     2581 cgagcacgac cgg
//
LOCUS       DASSQV010000044         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2376820, whole genome shotgun sequence.
ACCESSION   DASSQV010000044 DASSQV010000000
VERSION     DASSQV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376820"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VFL04_00790"
     CDS             complement(<1..1181)
                     /locus_tag="VFL04_00790"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET7838263.1"
                     /translation="MSASVLKVEGIAKSFGGVQALKGVSLELGQGEIHCLAGENGSGK
                     STLIKVISGAHAPDAGSIEIGGQRFSRLSPKEAIALGVQVIYQDFSVFPNLTVMENLA
                     LNMELMDKRRLMNYRRARSIAAHAVSLIGFRPDLDERVENLSVADRQLIAICRALLQD
                     SKLIIMDEPTSALTKKEVKALFAVIKSLQTRGLSILFVSHKLDEVFEISERYTILRSG
                     ENVASGLTTELDHASFAFHMTGREFAAASYRPAATGAEPILSVRGLSRRGAYSDVSFD
                     LAPGEILGITGLLGSGRTELVETIFGILKADSGTIEVGGKPARIGDVKTAIALGIGYV
                     PSDRLTEGLFQPQTIERNAVVAVLDRLCDRLGFLRAKAVAETTARWISELSIATKDPS
                     LP"
     gene            complement(1318..>2241)
                     /locus_tag="VFL04_00795"
     CDS             complement(1318..>2241)
                     /locus_tag="VFL04_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7838264.1"
                     /translation="KEWKIALVPKDSTNAWFVRMEVGVKQFAKDTGINVFQKGPPKTD
                     AAMQAQVIEDLIAQGVDAICVVPVDPAALEPVLGQALAKGIVVIDHEGATQQNCLYDI
                     EAFSNEGLGGAIMQVLAKDMGEKGVYTTMVGNVTNASHNEWADAGIAYQKAHYPNMTL
                     LAAEPRVEIMDNLDTAYQRAKELFKKYPNLKGVFGTSSLSTPGTARAIEELGLKGKAF
                     VTSVGLPNENKPYLKSGTVSGVLLWDPKDAGYAQLALAVAILKGEKIGNGLNLKAPGW
                     TSMQFAPGSTKVLQGKGWITINKANVDSFGF"
BASE COUNT          333 a          692 c          823 g          393 t
ORIGIN      
        1 ggaagcgagg ggtccttggt agcgatggac agctccgaga tccagcgcgc cgtggtctcg
       61 gcgacggcct tggcgcgcag gaagcccagg cggtcgcaga gccggtcgag gacggcgacc
      121 accgcgttgc gctcgatcgt ctgcggctgg aagagcccct cggtgaggcg gtccgagggg
      181 acgtagccga tgcccagagc gatcgccgtc ttcacgtcgc cgatcctcgc cggcttcccc
      241 cccacctcga tcgtccccga gtcggccttg aggatgccga agatggtctc gacgagctcg
      301 gtgcgccccg agccgaggag gcccgtgatg ccgaggatct ccccgggcgc gaggtcgaag
      361 gagacgtccg agtaggcccc ccgccgcgag aggcccctga ccgagaggat gggctcggcg
      421 cccgtcgcgg ccgggcggta gcttgcggcc gcgaactcgc ggcccgtcat gtggaaggcg
      481 aaggaggcgt ggtcgagctc ggtggtcagg cccgaggcga cgttctcccc gctgcggagg
      541 atcgtgtagc gctcggagat ctcgaagacc tcgtcgagct tgtgcgagac gaagaggatc
      601 gagaggcccc gcgtctggag gctcttgatc acggcgaaga gggccttgac ctccttcttc
      661 gtgagggccg aggtgggctc gtccatgatg atgagcttcg agtcctggag gagggcccgg
      721 cagatggcga tgagctggcg gtcggccacc gagaggttct ccacccgctc gtcgagatcg
      781 ggcctgaagc cgatcaggga caccgcgtgc gcggcgatgg agcgggccct gcggtagttc
      841 atgagcctgc gcttgtccat gagctccatg ttgagcgcga ggttctccat gaccgtgagg
      901 ttggggaaga ccgagaagtc ctggtagatg acctggaccc cgagggcgat ggcctccttg
      961 gggctcaggc gggagaagcg ctgccccccg atctcgatgc tgcccgcgtc gggggcgtgc
     1021 gctcccgaga tgaccttgat gagggtggac ttgcccgagc cgttctcgcc ggcgaggcag
     1081 tggatctcgc cctggcccag ctcgaggctg acccccttga gcgcctgcac gccgccgaag
     1141 gacttggcga tgccctcgac cttgaggact gaagccgaca tctctgcctc cagggatgaa
     1201 tatatcaacg ggacgcggca ggggaccttg ggtccccctc cgcgccccga tgggagccgc
     1261 ggcccgaggc ggccgtgcgg caggaccgca cccgaccgcc cgagagccta aagccgatca
     1321 gaagccgaag ctgtcgacgt tggccttatt gatggtgatc cagcccttgc cctggaggac
     1381 cttggtggag ccgggggcga actgcatgct ggtccagccc ggggccttga ggttgagccc
     1441 gttgccgatc ttctcgccct tgaggatggc gaccgcgagg gcgagctggg cgtagccggc
     1501 gtccttgggg tcccagagca ggacgcccga taccgtgccg ctcttgaggt agggcttgtt
     1561 ctcgttcggc aggccgaccg aggtcacgaa ggccttgccc ttgaggccga gctcctcgat
     1621 ggcgcgggcg gtgccgggcg tggagagcga ggaggtgccg aagacgccct tgaggttcgg
     1681 gtacttcttg aacagctcct tggccctctg gtaggcggtg tcgaggttgt ccatgatctc
     1741 gacgcggggc tcggcggcga ggagggtcat gttcgggtaa tgggccttct ggtaggcgat
     1801 gccggcgtcg gcccactcgt tgtgggaggc gttggtgacg ttgccgacca tcgtggtgta
     1861 gacgcccttc tcgcccatgt ccttggcgag gacctgcatg atggcgccgc cgaggccctc
     1921 gttcgagaag gcttcgatgt cgtagaggca gttctgctgg gtcgcgccct cgtggtcgat
     1981 gacgacgatg cccttggcga gggcctggcc gaggacgggc tcaagggccg ccggatcgac
     2041 cgggacgacg cagatggcgt cgacgccctg ggcgatcagg tcctcgatga cctgggcctg
     2101 catggccgcg tcggtcttgg gcgggccctt ctggaagacg ttgatgcccg tgtccttggc
     2161 gaactgcttg acgccgacct ccatgcgaac gaaccaggcg ttggtgctgt ccttcggcac
     2221 cagcgcgatc ttccactcct t
//
LOCUS       DASSQV010000045         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2252328, whole genome shotgun sequence.
ACCESSION   DASSQV010000045 DASSQV010000000
VERSION     DASSQV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2252328"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /gene="dnaK"
                     /locus_tag="VFL04_00800"
     CDS             complement(<1..1397)
                     /gene="dnaK"
                     /locus_tag="VFL04_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET7838265.1"
                     /translation="MGRIIGIDLGTTNSCVAVMEGGEPVVIANSEGQRTTPSIVGFTT
                     KGERVVGQPAKNQIITNPENTVYSIKRFMGRRFAEVQDEVKLVPYHVLDNGNDVRVDI
                     SGKGYSPQEVSAFILQKMKKTAEDYLGEAVTEAVITVPAYFNDSQRQATKDAGRIAGL
                     EVKRIINEPTAASLAYGYNKDQKKEKVIAVYDLGGGTFDISILELGEGVFEVKSTNGD
                     THLGGDDFDRRIMEWLVAEFKKDSGIDLSKDRMALQRLKDVAEKAKIELSNVQQTEIN
                     LPFITADASGPKHLQKTLTRSAFERMIDDLLDRSREPCKKALADAGLSAEQIDEVILV
                     GGSTRIPKVQTIVRDLFGKEPSKGVNPDEAVAVGAAIQGGILGGDVKAVLLLDVTPLS
                     LGIETLGGVFTRLITRNTTIPCRKSQIFSTAADGQTAVSIHVLQGEREMAGQNRTLGR
                     FDLVGIPPAPRGVPQ"
     gene            complement(1444..2250)
                     /locus_tag="VFL04_00805"
     CDS             complement(1444..2250)
                     /locus_tag="VFL04_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET7838266.1"
                     /translation="MSSKHHEPHDQHRKEERPQDQDAATPGTEDLAAPSRGAECLSED
                     EAVAVEGRALSAEARLELLQSELLAAKTELSAVNDKYLRKLADDVNFRKRMAREKEDS
                     QRFAVASLLGDLIPVLDDFDRAVMSAETARDYTILHDGIVLIRRQLGQLLENKYGLKR
                     LEALGKAFDPNHHEAVAMELVDEVSEPIVAEEFLPGSGVHDRVIRTAKVKVKMPGRPA
                     SQMEQQGSPDGSPDAGPAAQQDPSDEPRGEMRGGARDDQADAADGAAGTV"
BASE COUNT          343 a          734 c          757 g          477 t
ORIGIN      
        1 tcctggggca ccccgcgcgg ggcgggaggt atgccgacga ggtcgaagcg cccgagggtc
       61 cggttctggc cggccatctc gcgctcgccc tggaggacgt ggatcgacac cgcggtctgc
      121 ccgtcggcgg ccgttgagaa gatctggctc ttgcggcagg ggatcgtggt gttccgcgtg
      181 atcaggcggg tgaagacgcc gcccagggtc tcgatgccga gcgagagggg ggtgacgtcg
      241 aggaggagga cggccttcac gtctccgccg aggattccgc cctggatggc cgcgcccacg
      301 gccacggcct catcgggatt gacgcccttc gagggctcct tgccgaagag gtcgcgcacg
      361 atggtctgga ccttcgggat gcgggtggag ccaccgacga ggatgacctc gtcgatctgc
      421 tcggccgaaa gcccagcatc ggcgagggcc ttcttgcagg gctcccgcga gcggtcgagg
      481 agatcgtcga tcatgcgctc gaaggccgag cgggtgaggg tcttctggag gtgcttgggc
      541 ccggaggcgt cggcggtgat gaaggggagg ttgatctcgg tctgctggac gttcgagagc
      601 tcgatcttcg ccttctctgc gacgtccttg aggcgctgga gggccatgcg gtccttggag
      661 aggtcgatcc ccgaatcctt cttgaactca gcgaccagcc actccatgat gcgcctgtcg
      721 aaatcgtcgc cgccgaggtg ggtgtcgccg ttcgtcgatt tgacctcaaa gacgccctcg
      781 ccgagctcga ggatggatat gtcgaaggtg ccgccgccga ggtcgtacac ggcgattacc
      841 ttctccttct tctggtcctt gttgtagccg taggcgaggg acgcggccgt gggctcgttt
      901 atgatgcgct tgacctcgag gccggcgatc cggccggcgt ccttggtggc ctggcgctgg
      961 gagtcgttga agtaggcggg gacggtgatg acggcttccg tgaccgcctc accgagatag
     1021 tcctcggcgg tcttcttcat cttctgcagg atgaaggccg agacctcctg cgggctatag
     1081 cccttgccgg aaatgtccac cctcacgtcg ttgccattgt ccaggacatg gtagggcacg
     1141 agcttgacct cgtcctggac ctccgcgaag cggcgcccca tgaagcgctt gatagaatag
     1201 accgtgttct cgggattcgt gatgatctgg ttcttcgccg gctggccgac cacccgctca
     1261 cccttcgtcg tgaagccgac gatcgagggg gttgtgcgct gcccctcgga gttggcgatg
     1321 actacgggct ccccgccttc catgacagcg acacaggaat tggtcgtccc gagatcgatt
     1381 ccgattatcc tacccatttc ttcgctcctt cattcccatc atatacccag tgtcacgacc
     1441 gctctagacg gtcccggctg ccccgtccgc tgcgtcggcc tggtcatccc tggcaccgcc
     1501 cctcatctcg ccccggggct catcgctcgg atcctgctgg gccgcgggcc cggcgtccgg
     1561 agagccgtcc ggggatccct gctgctccat ctgcgaggcg ggtcgcccag gcatcttcac
     1621 cttgaccttg gccgtcctga tcactcgatc gtgcaccccg gagcccggca ggaactcctc
     1681 ggccacgatg ggctcggaga cctcgtccac gagctccatg gcgaccgcct cgtggtggtt
     1741 gggatcgaaa gctttcccaa gcgcctcgag gcgcttgagg ccgtacttgt tctcgagaag
     1801 ctgtccaagc tgacgcctga tgaggacgat cccatcgtgc aggatggtgt agtcgcgcgc
     1861 cgtttccgcg ctcatgactg cccggtcgaa atcgtcgagg acggggatca ggtcccccag
     1921 gagggaggct acggcaaaac gctggctgtc ttccttttcg cgcgccatgc gcttccggaa
     1981 gttgacgtca tccgcgagtt ttctgaggta cttgtcattg acggctgaga gttcggtctt
     2041 ggcggcgagc agctccgact ggagcagctc aagcctcgcc tcggcgctca gggcgcgccc
     2101 ctcgaccgcg acggcctcgt cctccgaaag acattccgcc ccccggctcg gagccgctag
     2161 gtcctcggtc cccggcgtcg cggcgtcctg atcttgtgga cgctcttcct tcctgtgctg
     2221 gtcgtgaggc tcatggtgct tgctcgacat cgttccacct gggatcaacg gcctaggctg
     2281 ccgcttgagg tatcaataaa gtaataatga t
//
LOCUS       DASSQV010000046         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2377092, whole genome shotgun sequence.
ACCESSION   DASSQV010000046 DASSQV010000000
VERSION     DASSQV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2377092"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFL04_00810"
     CDS             <1..502
                     /locus_tag="VFL04_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7838267.1"
                     /translation="SSFGLEHWRFIAVTNPAMRARLKHACFEQEAVASSPFVVAILVR
                     RAAAYEPHSDFVRSRAERFPGGWPVFFEDYRGYYEYLAGGGRLDHWARAQAYIACANM
                     MTGAAAAGIDSCAIEGFDEAASLAVLEAEPEEWTVGLFAVFGHAAEEARAKIREGLPS
                     IAERRD"
     gene            complement(499..891)
                     /locus_tag="VFL04_00815"
     CDS             complement(499..891)
                     /locus_tag="VFL04_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838268.1"
                     /translation="MTRAEFAAELGGIIRLLREHSFLAYSASGRREYEQKLRTCGDYF
                     AYLVFRKTGIPVRSLPGLVPIESAREANYATGRRLVKRAAANARATAEKCSGLDRDYY
                     LGLALLWERAAERLEEPEPRNRAGRNRS"
     gene            complement(894..2141)
                     /gene="tyrS"
                     /locus_tag="VFL04_00820"
     CDS             complement(894..2141)
                     /gene="tyrS"
                     /locus_tag="VFL04_00820"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET7838269.1"
                     /translation="MNAALATLKERGFLQQCTDIEALSKVMDEGPITFYEGCDPTGPS
                     LHIGHMVPYFAIRHLHEAGHRAIVLMGGGTARIGDPSGKSSLRQIISYEEIDANTKLF
                     LGQIDRFLKFDGATLYSANNRDWLADLNYIDFLRDIGRHFSVNRMLSFEAYKQRMETG
                     LSFIEFNYQLLQSYDFLQLYKRHGCRLQIGGDDQWGNIVAGIELIRRVEGAECFGLTF
                     PLVMTADGKKMGKTEKGALFLDPKMTSPYDFFQYWRNVADGDVERFLLMFTFLPVSEC
                     LELGRAKDAAINAAKERLAWEVTALIHGNEEADRALAAAKAAFGGRGGATGGGDIGQV
                     PSREMPRAELEKGMGVLELFVFAGLAASKSEARRLVQQGGALVGERKIDDETAVIDAS
                     CLDADGAVMLRAGKKRFFRIIAK"
BASE COUNT          325 a          721 c          737 g          386 t
ORIGIN      
        1 aagctccttc ggcctcgagc actggcgctt catcgcggtc acgaatccgg ccatgcgggc
       61 gaggctcaag catgcctgtt tcgaacagga ggccgtggcc agctcgccct tcgtcgtcgc
      121 catcctcgtg aggcgcgcgg ccgcctatga gccgcactcc gatttcgtca ggtcgcgggc
      181 cgagcgcttc ccgggaggct ggcccgtctt cttcgaggac tatcggggct attacgagta
      241 cctcgccggc ggagggaggc ttgaccactg ggcgagggcc caggcctaca tcgcctgcgc
      301 gaacatgatg acgggggcgg cggccgcggg gatcgactcc tgcgccatcg agggcttcga
      361 cgaggcggcg agcctcgcgg tccttgaggc cgagcccgag gagtggacgg tggggctctt
      421 cgccgtcttc ggccacgccg ccgaggaggc ccgagccaag atcagggagg gcctcccttc
      481 catcgcggag cggagggact aggaccggtt cctcccggcc cggttcctcg gctcgggctc
      541 ctcgagcctc tccgctgccc gctcccagag cagggcgagc ccgaggtagt agtcgcggtc
      601 caggcccgag catttctctg cagtcgcgcg cgcgttcgcg gccgcccgct tcacgagccg
      661 ccttcccgtc gcgtagttcg cctccctcgc cgactcgatg ggcacgagcc ccgggaggga
      721 gcgcacgggg atgccagtct tcctgaagac gaggtaggcg aaatagtcgc cgcaggtcct
      781 gagcttctgc tcgtattccc gcctcccgga ggcggagtag gcgaggaagg aatgctcgcg
      841 gaggaggcgg atgatgcccc cgagctcggc cgcgaactcc gccctggtca tccctatttg
      901 gcgatgatcc ggaagaagcg cttcttgcca gccctcagca tgacggcgcc gtccgcgtcg
      961 aggcaggacg cgtcgatcac ggccgtctcg tcgtcgatct tccgctcccc gacgagggcc
     1021 ccgccttgct gaacgaggcg gcgcgcctcg ctcttggagg cggccaggcc cgcgaagacg
     1081 aagagctcga ggacccccat gcccttctcg agctcggccc tgggcatctc gcgcgagggc
     1141 acctgcccga tgtccccgcc gcccgtggcc ccgcctcggc ccccgaaggc cgccttcgcg
     1201 gcggcgaggg ccctgtcggc ctcttcgttg ccatggatga gggcggtgac ctcccaggcc
     1261 agcctctcct tggcggcgtt gatcgcggcg tccttcgccc gccccagctc aaggcattcg
     1321 gagaccggga ggaaggtgaa catgaggagg aagcgctcga cgtcgccgtc ggccacgttg
     1381 cgccagtact ggaagaagtc gtaggggctc gtcatcttcg ggtcgaggaa aagggcgccc
     1441 ttctcggtct tgcccatctt cttcccgtcc gccgtcatga cgagggggaa ggtgaggccg
     1501 aagcactcgg cgccctccac gcggcggatc agctcgatcc ccgcgacaat gttcccccac
     1561 tggtcatcgc ccccgatctg gaggcggcag ccgtggcgtt tgtacagctg caggaagtcg
     1621 tagctctgga ggagctggta gttgaactcg atgaaggaga ggccagtctc catcctctgc
     1681 ttgtaggcct cgaaggagag catcctgttg accgagaaat ggcggccgat gtcgcggagg
     1741 aagtcgatgt agttcaggtc ggcgagccag tccctgttgt tcgccgagta gagggtcgcg
     1801 ccgtcgaact tgaggaacct gtctatctgg ccaaggaaga gcttcgtgtt cgcgtcgatc
     1861 tcctcgtagc tgatgatctg gcgcaggctc gacttgcccg agggatcgcc gatccgggcc
     1921 gtgcctccgc ccatgaggac gatggcgcga tgcccggcct cgtggaggtg gcggatcgcg
     1981 aagtagggca ccatgtggcc gatgtggagg gagggccccg tcggatcgca gccctcgtag
     2041 aaggtgatcg gcccctcgtc catcaccttc gagagcgcct cgatgtcggt gcactgctgc
     2101 aggaaacctc gttccttgag cgtcgctagg gctgcgttca tttccttcct ccgcgtgccg
     2161 cattatagc
//
LOCUS       DASSQV010000047         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_511672, whole genome shotgun sequence.
ACCESSION   DASSQV010000047 DASSQV010000000
VERSION     DASSQV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511672"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /gene="fliN"
                     /locus_tag="VFL04_00825"
     CDS             <1..733
                     /gene="fliN"
                     /locus_tag="VFL04_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HET7838270.1"
                     /translation="AISAVQECISTLTGNQITVISEKTGNRNIQAGVAEGSFGPKAMA
                     QVPAGDFLRAVYQVQIGEGAPERMLELFALSFVKDVLASLGGAQMGGQVQAAPRAAMM
                     AQPQQQPMMMGGGFGQMGGGYNPMQGFQAPMGGMNPPNVQPIQFASLQMQQASPESGN
                     IGLIMDVFMEMTVELGRTRKLIKEILGMGEGTIIELDKLAGEPVDILVNHKLIAKGEV
                     VVIDENFGVRVTEIVSPMERMGDLG"
     gene            859..1452
                     /gene="fliO"
                     /locus_tag="VFL04_00830"
     CDS             859..1452
                     /gene="fliO"
                     /locus_tag="VFL04_00830"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HET7838271.1"
                     /translation="MALGSGAVAGQGTKAPTPEAGGVVGTAQTVDETTLVIGDRATAA
                     AAGSVPAGPSTLAYFLRMVVVLALVLGAIYGAYRLMRRASRPRIEEDPTVKVLATASL
                     GPGKAVHVLSVGNQAFLIGVTDSSISLISEVADRDLVDSLALKAAMAPKAREGLGGFG
                     EALAALLAGKGRAAGKRGPQPGDGDFLARQRERLRKF"
     gene            1462..2364
                     /gene="fliP"
                     /locus_tag="VFL04_00835"
     CDS             1462..2364
                     /gene="fliP"
                     /locus_tag="VFL04_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707387.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HET7838272.1"
                     /translation="MRAKTAIVPAILCGLCLALGSGRAFAQTAPRSPLPAIQVPTPPP
                     PQGAAGTAQRSPAFPGASARGGTGLPAQVPAIPLVDLRVRNPASGQEVAFSVQLLLLL
                     TALSLAPSILILLTSFLRISIVLDFVKRALSLQQVPPNQVLLGISLFLTIFIMWPTFD
                     TVYKDSLKPLSEGRIGVEQAYVAAEKPFRIFMFNQMRSNPDNIRLFMSMRGLPKPTSL
                     ADVPTYVLVPAFVLNEMTVAFKIGILLFIPFIIIDMVVASVLMSMGMIMLPPVMISMP
                     FKLILFILVDGWGLLTQQLVRSFV"
     gene            2378..>2542
                     /locus_tag="VFL04_00840"
     CDS             2378..>2542
                     /locus_tag="VFL04_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HET7838273.1"
                     /translation="MGVGEAVSIMRGGIVETMILAAPVLLVAMVVGLVVSILQATTSI
                     QEQTLTFVPKI"
BASE COUNT          423 a          878 c          798 g          443 t
ORIGIN      
        1 ggccatctcg gccgtgcagg agtgcatctc gaccctcacc ggcaaccaga tcaccgtgat
       61 ctcggagaag accggcaacc gcaacatcca ggccggggtc gccgagggca gcttcggccc
      121 caaggcaatg gcccaggtgc cggcagggga tttcctcagg gccgtctacc aggtccagat
      181 aggcgagggg gcccccgagc ggatgctcga gctcttcgcc ctctccttcg tgaaggacgt
      241 cctcgcatcc ctgggcgggg cccagatggg gggccaggtc caggccgccc cgcgcgccgc
      301 gatgatggcc cagccccagc aacagccgat gatgatgggc gggggcttcg gacagatggg
      361 cgggggctac aacccgatgc agggtttcca ggcgcccatg ggcgggatga acccgcccaa
      421 cgtccagccg atccagttcg ccagcctcca gatgcagcag gcctcgcccg agtcggggaa
      481 catcggcctc atcatggacg tcttcatgga gatgacggtc gagctgggcc ggaccaggaa
      541 gctcatcaag gagatcctgg gcatgggcga gggcacgatc atcgagctgg acaagctggc
      601 cggcgagccc gtagacattc tggtcaacca caagctgatc gccaagggcg aggtcgtcgt
      661 catcgacgag aacttcggcg tgcgcgtgac cgagatcgtc agcccgatgg agcgcatggg
      721 ggacctcggc taaaatttta tccgcctgta aaatagcgcg atattcgctt gcgtttccct
      781 cccgtatgta ctaaaataat acccgtattg ggaggggatc ttttgaaagg catagcgctc
      841 gtcctatgcg ccacgctcat ggcgctcggg tcgggggccg tcgcgggtca gggcacgaaa
      901 gcgcccacgc ccgaggccgg aggggtcgtg gggacggccc agacggtcga tgagacgact
      961 ctcgtcatcg gcgacagggc gacggcggca gccgcgggta gcgtacccgc gggtccaagt
     1021 acattggcct atttcctccg catggtggtg gtcctcgctc ttgtgctggg ggccatctac
     1081 ggggcctatc ggctcatgcg ccgcgcatcg cgtccgcgga tcgaggagga cccgaccgtc
     1141 aaggtcctcg ccacggcctc gctcggcccg ggcaaggccg tgcacgtcct gtccgtaggc
     1201 aaccaggcct tcctgatcgg cgtcaccgac agctccatat ccctcatctc ggaggtggcc
     1261 gacagggacc ttgtggacag ccttgcgctg aaggccgcca tggccccgaa ggccagggaa
     1321 ggcctcggcg gtttcggcga ggccctcgcc gccctccttg ccggcaaggg cagggccgcg
     1381 ggcaagcgcg ggccgcagcc cggcgacggg gacttcctcg cgcgtcagcg cgagcgtctc
     1441 cggaaattct aggggcatcc catgcgcgca aaaaccgcca tagtacccgc catcctctgc
     1501 gggctctgcc tggccctcgg ctcgggcagg gccttcgccc agaccgcccc gcgctccccg
     1561 cttccggcga tccaggtccc gacgcctccg cctccccagg gcgcggcagg gacggctcag
     1621 cggagccctg ccttcccggg cgcgagcgcc agggggggca cggggcttcc cgcccaggtg
     1681 cccgccatcc ccctcgtcga cctcagggtc aggaacccgg cctcgggcca ggaggtggcc
     1741 ttctcggtcc agctcctctt gctcctcacg gccctctccc tggccccgag catcctcata
     1801 ctgctgacga gcttcctccg catatccatc gtcctcgact tcgtgaagcg cgccctctcg
     1861 ctccagcagg tcccgccgaa ccaggtcctc ctcggcatct cgctcttcct cacgatcttc
     1921 atcatgtggc cgaccttcga cacggtgtac aaggactcgc tcaagcccct gtccgagggg
     1981 aggatcggcg tcgagcaggc ctacgtggcg gccgagaagc ccttcaggat cttcatgttc
     2041 aaccagatgc ggtccaaccc cgacaacatc aggctcttca tgtcgatgcg cggcctgccg
     2101 aagccgacca gcctcgccga cgtgccgacc tatgtcctcg tgccggcctt cgtcctcaac
     2161 gagatgaccg tggccttcaa gatcggcatc ctcctgttca tacccttcat catcatcgac
     2221 atggtcgtcg cctcggtgct catgtccatg ggcatgatca tgctgcctcc cgtcatgatc
     2281 tccatgccat tcaagctcat cctgttcata ctcgtcgacg gatggggcct cctgacccag
     2341 cagctggtca ggtccttcgt gtgagagggg gtaggagatg ggtgtaggag aagcggtatc
     2401 catcatgagg ggggggatcg tcgagacgat gatcctcgcc gccccggtcc tcctggtcgc
     2461 catggtcgtc ggcctcgtgg tctcgatcct ccaggccacc acctcgatcc aggagcagac
     2521 actcaccttc gtcccgaaga ta
//
LOCUS       DASSQV010000048         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_636209, whole genome shotgun sequence.
ACCESSION   DASSQV010000048 DASSQV010000000
VERSION     DASSQV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636209"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFL04_00845"
     CDS             <1..578
                     /locus_tag="VFL04_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838274.1"
                     /translation="FLGEIVQPGTNDATPAYRTRRLFIESELIPLEAVMWELGRAKAF
                     KVKQLVSNTWIGETGRGYLVRGIWMDNDLSKFFSKLVLPDGAKTYPFYPLFCKYKTIC
                     AETPQPGPEGRKRMLPLLHRAIGLLAPEMERIQNSLRSSPFSETMPEYAELRSRVPPN
                     WREVLRGVSTRAYLNDRDMKEFSLEFAAAVS"
     gene            553..1956
                     /gene="murD"
                     /locus_tag="VFL04_00850"
     CDS             553..1956
                     /gene="murD"
                     /locus_tag="VFL04_00850"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET7838275.1"
                     /translation="MNSPLQSLEHIPGLRVTIMGLGLNGGGLASALFFARRGATVTVT
                     DMKGEEALAEPVAALAGLPVRFVLGRHELDDFRNADVVIKNPAVRPDSPFLLASKAVE
                     TDLSVFLRFSESPLIAVTGSKGKSSTSSAIHWGLVAAGRTAFLGGNITVSPLSFLDRT
                     GPGTPVVLELSSWQLADLKGMGVLKPAVALLTSIRPDHMNRYGSMDEYVADKRLIYAD
                     QDQACYTLCNRDDPWGLSFAAETRARVRWYGERDEGLAGAWLDPATGRGYFSDDRGGG
                     AKPEEILGPELIVPGFHQRKNLLGAALALRLFGVEAPSIRAAMASFPGVEHRLEFFAE
                     KDGVRWFNDSAATIPQAVGAALGSFDGPIVLITGGTDKNLDFEPVRPAYGKPRLIVLL
                     AGSGTEKLRALLDADGIAYLGPYDELGPAVREAASRARDGDTVLLSPGCTSFGMFLHE
                     FDRGRKFKETVRSLLGV"
     gene            2000..>2619
                     /locus_tag="VFL04_00855"
     CDS             2000..>2619
                     /locus_tag="VFL04_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7838276.1"
                     /translation="MSEDRHEGQTPEPSGQGPGLPLAAHEQAVEIRLPVRHNPILKSV
                     VERVNADEELFALWRAANVNAVDRLGMTDHGPVHFQIVSNIALKMLRQLVDCGVVPNI
                     VADHGMASEDAEVVVVLGALLHDLGMSIQRAEHEQYSLFVAQPIVDRLLQGLYATGPR
                     TIVRSETLHAIISHRSDGRPMTVEAGIVRVADALDMTKGRSRIPFEA"
BASE COUNT          414 a          985 c          812 g          408 t
ORIGIN      
        1 ccttccttgg cgagatcgtc cagccgggga ctaacgacgc caccccggcc taccgcaccc
       61 ggcgcctctt catcgagagc gagctcatcc ccctcgaggc cgtcatgtgg gagctcggca
      121 gggccaaggc cttcaaggtg aagcagctgg tctccaacac ctggataggg gagacgggcc
      181 gcggctacct cgtccgcggc atctggatgg acaacgacct ctcgaagttc ttctcgaagc
      241 tcgtcctgcc cgacggggcc aagacctacc ccttctatcc gctcttctgc aagtacaaga
      301 cgatctgcgc cgagactccc cagcccggcc ccgagggcag gaagcggatg ctccccctcc
      361 tgcacagggc gatcggcctt ctcgcccccg agatggaaag gatccagaac tccctccgca
      421 gctcgccctt ctccgagacg atgcccgaat acgccgagct caggagccgt gtgcccccga
      481 actggcgcga agtcctgcgg ggcgtgtcga cccgcgccta tctcaacgac cgcgacatga
      541 aggagttctc gcttgaattc gccgctgcag tctcttgagc acatccccgg cctgagggtc
      601 acgatcatgg gccttggcct caacgggggc ggcctcgcga gcgccctctt cttcgcccgg
      661 aggggggcca cggtcacggt gaccgacatg aagggcgagg aggccctcgc ggagccggtc
      721 gcggccctcg ccggcctccc tgtgcgtttc gtcctgggcc gccacgagct cgacgatttc
      781 cgcaacgccg acgtcgtcat caagaacccc gccgtccgcc ctgactcgcc cttcctcctc
      841 gcatcgaagg ccgtcgagac cgacctctcg gtcttcctcc gcttcagcga atcccccctc
      901 atcgcggtca cgggctccaa gggcaagtcc tcgacctcga gcgcgatcca ctggggtctt
      961 gtggccgctg ggaggacggc cttcctcggg gggaacatca ccgtatcccc actgagcttc
     1021 ctcgaccgca cgggccccgg gacccccgtc gtgctcgagc tctcgtcctg gcagctcgcc
     1081 gacctcaagg gcatgggggt gctcaagccg gccgtcgccc tcctcacgag catcaggccc
     1141 gaccacatga accgctacgg ctccatggac gagtacgtcg ccgacaaacg gctcatctac
     1201 gccgaccagg accaggcctg ctacaccctc tgcaaccgcg acgacccctg gggcctctcc
     1261 ttcgccgccg agaccagggc ccgggtccgc tggtacggag agcgcgacga gggcctggcc
     1321 ggggcctggc tcgatccggc cacgggacgg ggatacttct ccgatgaccg cgggggaggg
     1381 gccaagcccg aggagatcct cgggcccgag ctcatcgtgc cgggctttca ccagcgcaag
     1441 aacctcctcg gcgccgccct cgccctccgc cttttcggcg tcgaggcccc atccatcagg
     1501 gccgccatgg ccagtttccc tggggtcgag caccgcctcg agttcttcgc ggagaaggac
     1561 ggggtgcgct ggttcaacga ctcggccgcc accatccccc aggcggtggg cgccgccctc
     1621 ggaagcttcg acgggcccat cgtcctcatc accggcggca ccgacaagaa cctcgatttc
     1681 gagccggtgc gtcccgccta cgggaagccg cgcctcatcg tcctgctcgc gggctcgggc
     1741 acggagaagc tcagggccct ccttgacgcc gacgggatcg cctacctcgg gccctatgac
     1801 gagctggggc ccgcggtccg ggaagccgca tcgcgggcca gggacgggga caccgtcctc
     1861 ctttcgccgg gctgcacctc cttcggcatg ttcctccacg agttcgaccg cgggaggaag
     1921 ttcaaggaga ccgtgcgctc cttgctcggg gtctaggccc tcctttgcgc tttccctccg
     1981 atggggtaga gttccccaca tgagcgaaga ccgacacgaa gggcagacgc ccgagccgag
     2041 cggccagggc cccggcctcc cgctggcggc ccacgagcag gccgtcgaga tccgcctgcc
     2101 cgtcaggcac aaccccatcc tcaagagcgt cgtggagcgc gtgaacgccg acgaggagct
     2161 ctttgcgctc tggagggcgg ccaacgtcaa cgccgtggac aggctcggca tgaccgacca
     2221 cgggcccgtg catttccaga tcgtctccaa catcgcgctc aagatgctgc ggcagctcgt
     2281 ggactgcggg gtggtgccga acatcgtcgc cgaccacggc atggcgagcg aggacgccga
     2341 ggtcgtcgtc gtcctcggcg ccctcctcca cgacctcggc atgtccatcc agcgcgccga
     2401 gcacgagcag tacagcctct tcgtggccca gccgatcgtc gaccgcctcc tccagggtct
     2461 ctacgccacg gggccccgca ccatcgtgcg gtcggagacc ctccacgcga taatctcgca
     2521 ccggagcgac ggccgcccca tgaccgtcga ggcggggatc gtgcgcgtgg ccgacgccct
     2581 cgacatgacg aagggccgct cgcgcatccc cttcgaggc
//
LOCUS       DASSQV010000049         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2128859, whole genome shotgun sequence.
ACCESSION   DASSQV010000049 DASSQV010000000
VERSION     DASSQV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2128859"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFL04_00860"
     CDS             <1..804
                     /locus_tag="VFL04_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7838277.1"
                     /translation="GPGLGRPARYPANPGGGRLRRLLTATASAVLIIMAWKLAAVAIG
                     KDIVLPPPERVFGLAVGLYPSTKFLDALAATFLRGTAAFALSAAAGTALGLAAGTWPL
                     ASAALAPILTVIRATPVLALILLALIWFPSGFVPIFSAFLMAFPVMVSSAAEGARASD
                     PRLLEMAGLFRVPRGAVFWKLRLPSAAPHLLAGARSALGLSWKVVVAGEVLSQPVRAL
                     GTGMQDARVMLETGSVLAWAAASVLLCGLTEWAFGLLVRGRGRDGLRTA"
     gene            785..1501
                     /locus_tag="VFL04_00865"
     CDS             785..1501
                     /locus_tag="VFL04_00865"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7838278.1"
                     /translation="MAYELREVCAGYGGEPVLAGLTLALRPGSITVVLGPSGCGKTTL
                     LNLIAGLKTPDSGHRLGFEGVRFSYAFQEPRLLPWLCARDNALFALEAALPRDEALGR
                     VELFLAAAGLAEAASKRPAELSGGMRQRLSLVRAFAFPSEILLLDEAFQAVDLATKLE
                     LMDSFMRLWREEKRTVVLVTHDVEEAVYLADRVIVLSKRPAAIVADFPVEVPRADRAF
                     GSTATMEAESRLYRLVLGGT"
     gene            complement(1516..>2034)
                     /locus_tag="VFL04_00870"
     CDS             complement(1516..>2034)
                     /locus_tag="VFL04_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7838279.1"
                     /translation="GIAKQSGGAVGFKCFDEGGTMFTVWLPLALARPEPSMIPQEPPA
                     GAAAAAATILFVDDDESLRSLASRLLAKRGHRVLVAANAGEALLIAESYGQPIDLLVS
                     DTVMPFMDGYTLAKRLETILPGIGLLFVSGHPERSADPEASGRFLAKPFTENELARAV
                     AEALERRKTAAT"
BASE COUNT          255 a          729 c          704 g          346 t
ORIGIN      
        1 gggccgggtc tcggcaggcc tgcgcgatat cccgcaaacc cgggcggagg gaggctgcgg
       61 cgcctgctca ctgccacggc gagcgccgtc ctcatcatca tggcctggaa gctcgccgca
      121 gtggccatcg gcaaggacat cgtgctcccc cctccggagc gggtcttcgg cctcgccgtc
      181 ggcctgtatc ccagcacgaa attcctcgat gcgctcgcgg cgaccttcct ccgcgggact
      241 gcggccttcg ccctctcagc cgcggcgggc acggccctcg gcctcgccgc ggggacctgg
      301 cccctggcct cggccgccct cgcccccatc ctcacggtga tacgggcgac ccccgtcctg
      361 gccctgatcc tcctcgccct gatctggttc ccttccggct tcgtccccat cttctcggcc
      421 ttcctcatgg ccttccccgt catggtgtcg agcgcagccg agggagcgcg ggcctctgat
      481 ccgcggctcc tcgagatggc gggcctcttc agggtgccgc ggggggccgt cttctggaag
      541 ctgaggctgc catcggcggc tccccatctt ctcgcgggcg ccaggagcgc cctcggcctc
      601 tcgtggaagg tcgtcgtcgc cggcgaggtc ctgagccagc ccgtgagggc cctcggcaca
      661 ggcatgcagg acgcgagggt catgctcgag acgggcagcg tcctcgcctg ggcggccgcc
      721 tcggtcctcc tctgcggcct caccgaatgg gccttcgggc tcctggtcag gggaaggggg
      781 cgcgatggcc tacgaactgc gtgaggtctg cgccggatac ggcggcgaac ccgtcctggc
      841 tggcctcacg ctcgccctcc ggcccggatc catcacggtc gtgctcggtc cctcgggctg
      901 cggaaagacc accctcctga acctgatcgc cggcctcaag accccggact cgggccatag
      961 gctcggcttt gaaggggtcc gtttctccta cgccttccag gaaccccgcc tcctgccctg
     1021 gctctgcgcc agggacaatg cccttttcgc tctcgaggcc gccctgcccc gagacgaggc
     1081 ccttggacgg gtggagctct tcctcgcagc ggccggcctg gccgaggccg cctccaagag
     1141 gccggccgag ctctcgggcg ggatgcgcca gcggctttcc ctggtcaggg ccttcgcctt
     1201 cccctcggag atcctcctcc tcgacgaggc ctttcaggcc gtcgaccttg ccaccaagct
     1261 cgagctcatg gactcgttca tgaggctctg gagggaggag aagcggacgg tcgtgctcgt
     1321 gacccacgat gtcgaggagg cggtctatct ggccgatagg gttatagtcc tttccaagcg
     1381 gccggcggcc atagtcgcgg acttccccgt ggaggtgccg agggcggaca gggcctttgg
     1441 ctcgaccgcg acgatggagg ccgagtcccg gctctaccgg ctcgtgctgg gcgggacctg
     1501 atctcggggc cggactcagg tggccgcggt cttgcggcgc tcgagggcct cggccaccgc
     1561 cctcgccagc tcgttctcgg tgaagggctt ggcgaggaag cggccgctgg cctcggggtc
     1621 cgccgacctc tcggggtggc ccgacacgaa gagcaggccg atccccggca ggatggtctc
     1681 gagacgtttc gcgagggtat agccgtccat gaagggcatc acggtgtccg agaccagaag
     1741 gtcgatgggc tggccgtagc tctccgcgat gaggagggcc tcgccggcgt ttgccgcgac
     1801 caggacgcga tgcccgcgtt tggcgaggag gcgcgaggcg aggctccgca gggattcgtc
     1861 gtcgtcgacg aagaggatgg tggcggcggc ggccgccgcc cccgcgggag gctcctgcgg
     1921 gatcatgctc ggctcgggcc tcgcgagggc gagggggagc cagaccgtga acatggttcc
     1981 gccctcatcg aagcatttga agccgacggc ccccccgctc tgcttggcga tccc
//
LOCUS       DASSQV010000050         7504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_263692, whole genome shotgun sequence.
ACCESSION   DASSQV010000050 DASSQV010000000
VERSION     DASSQV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263692"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFL04_00875"
     CDS             complement(<1..641)
                     /locus_tag="VFL04_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7838280.1"
                     /translation="MSDRRVRLAARGLSCGYAGRRVLEGVDLDLADGEVLALIGPNGS
                     GKTTLFRTLSGELRALAGTALVAPGPEDDLVETAELSRPARARTIARVLQAESPSWPA
                     TVREYVEGGTFAHTGWFGSPGEEEMKAVDDALMATGLEPFASRRVTELSGGEFRMVLI
                     ARALAQRPAVLLLDEPAAELDIARQMQVLGLLRELASRGVAVALSVHDLNLAAL"
     gene            complement(638..1708)
                     /locus_tag="VFL04_00880"
     CDS             complement(638..1708)
                     /locus_tag="VFL04_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7838281.1"
                     /translation="MKPGRTHRARLGAAIAALSFLLALLVSLCIGSSPLPPGQVLSAL
                     ATRLIPGLPAVSGTAADPMATVIVWDLRLARSLLAGVAGAALGAAGAVMQGLFRNPLA
                     DPYALGVSSGASLGAVAAISLGLSSPLVAIGATEILAFAGALGSAFMVYAIARSARRQ
                     PQTAALLLAGAAVGSFLGALVSLIVTLDSRDLQRVFFWLLGGFGGKSWPDLGAALPLA
                     AASIGLSSLLARPLDLLGAGEEAASSLGLDIERTRRLAVAASSLGVAAAVSAGGIIGF
                     VGLLGPHLARLLVGPGHRRLVPVSAFTGAALLMVADAVARSVASPLALPVGIVTAIVG
                     APLFLRILARRMPGEGPREAAR"
     gene            complement(1705..2625)
                     /locus_tag="VFL04_00885"
     CDS             complement(1705..2625)
                     /locus_tag="VFL04_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HET7838282.1"
                     /translation="MRRAFVGSGSFALVALAFLALSAADGAAQGVSAVDSLGRKVSLD
                     APAARIVSLSPAATEALFAVGAGPKLVGDTTYCDYPEEARSLPKVGGFVAETVSVERV
                     LALKPDLVVSGGSLHAGLEASLRRLGLRVFAYEPGDFRGVADAMMALGALSGTPGAAL
                     KAAAALTGAIKRITDATSAIPIAQRPRVLWLVYDDPLMTCGSVSLPHAILEAAGGRDI
                     FADLKGPWPQVSSEEVIRRAPEVILSPDDMGDKVSAARIGQRPGWSTVPAVRSGRILL
                     LPAALVSRAGPRVAAGVMAAAKALHPELFP"
     gene            complement(2636..3691)
                     /gene="cobT"
                     /locus_tag="VFL04_00890"
     CDS             complement(2636..3691)
                     /gene="cobT"
                     /locus_tag="VFL04_00890"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HET7838283.1"
                     /translation="MTAYEKTIAAIKPRDNAAAEAARDRQNRLTKPGGSLGRLEELSI
                     AIAGMTGQTRPRLGPGAVFVMAADHGVCAEGVSAFPQAVTAQMVQNFLRGGAAINVLA
                     RRAGARVVVVDVGVASEIPAAPNLYSRKVAPGTGNMARGSAMSRDQAMAAIQVGIEVF
                     EAELERSPFGIAATGDMGIGNTTASAAITAVLTGSDPATVVGRGTGVDDAGLARKTAA
                     ILRALSLNAPDARDPLGVLAAVGGFEIGAIAGSILAAAAHRIPILIDGYISGAGALVA
                     QGLCPLCAQYMIASHLSIEPGHRVVLTDLGLDPVLDLGLRLGEGTGAALAMMVCDAAC
                     RVLDEMATFDEAGVSGA"
     regulatory      complement(3728..3913)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3983..4465
                     /locus_tag="VFL04_00895"
     CDS             3983..4465
                     /locus_tag="VFL04_00895"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HET7838284.1"
                     /translation="MRTLITGGVKSGKSRHALELAMEFPEPRRFLATAEAFDDEMRGK
                     IARHRLERSDNFETIEEPIDIHERLAERMVLDCLPLWLNNLIHYGRESEFEVILSALI
                     GRLPRDIVIVSNEVGMGFVPADALSRRYGVLLGTANARLAAVCDSVVLMVAGVPLRVK
                     "
     gene            4476..5162
                     /locus_tag="VFL04_00900"
     CDS             4476..5162
                     /locus_tag="VFL04_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838285.1"
                     /translation="MRLCVPSYLVPGTWLENLEAMRDEVWIEGVELLFFAWDRDAQEI
                     LAREGEGIAALSSRFDFSLHLPDRLGPEALSLVEATESCVSSYIAHPPPSADDAGAWA
                     RLLGAWRERFGDRFLLEYTGATAFARGEEALPGLPLCADTGKLLVEGIEPSAWIGPRS
                     GRIGEVHLHGAEAGRDHRPFGAAAPWLRRLLPFLVGFEGRVNIEVFELEAARSAALAL
                     AAALAGRGGP"
     gene            5159..5923
                     /locus_tag="VFL04_00905"
     CDS             5159..5923
                     /locus_tag="VFL04_00905"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HET7838286.1"
                     /translation="MRVCLRRFLSVFSLVSRIPVRTAFECDYARADFWLPIVGVFASA
                     AALAGYGLASLVFASPLLRGLGALFVQYSAFNLFHLDGLLDSADAMTPYASRERRHEI
                     LKDPRIGSYALFYGFFALAAKLAALASLGPPAILALFLAYPAAGRLGAALLPALIGPA
                     REGGLGALMRGLSPLRSALGFALALMPLLAWSAASGAGRTGLAAAACAIAGAAIGALW
                     MALLYRRKLGGFTGDALGAAVELGELACVLLFAALA"
     gene            complement(5920..6306)
                     /locus_tag="VFL04_00910"
     CDS             complement(5920..6306)
                     /locus_tag="VFL04_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838287.1"
                     /translation="MNADVFTLCDFAQESDGKLTIVGAFDTIYARRFPAVHPAMCLAV
                     RLRFYIHEAGKHAIKIVFVAQDGTEAIKTIYGEAVVKDFTGSSRVIHSVFTLLNTPID
                     HEGTLSITLTVDDRELLTSPLYAKKI"
     gene            complement(6303..7397)
                     /locus_tag="VFL04_00915"
     CDS             complement(6303..7397)
                     /locus_tag="VFL04_00915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET7838288.1"
                     /translation="MRDKAGEPGADDLVRLSVFSSIVSGIMAEQSITGVLDRLMDELG
                     SYFGPHASSLLLLDSGMLNFASCRGPAADSLLGMAIPGDEGLAAWVAKAGLPLLVGDA
                     ASDARFSDRIDRAGAFRTRTSMLAPLRSEDRVFGVIELDCRQEDRSYAAADLLALSTI
                     VDFAAIAVEKAYYLAEARRLSELDPLTGARNRRGLRLALDRETLRAARYGGEFSVILA
                     DVEGFKAINETFGRAAGDSVLALVAETLIKTVRDVDSVARHGGDEFIVLLPATGPEEA
                     EIARLRLGTTLDALGRGHEPPFGVGLAVHSPEDLDLDALLREPDRGPHRLRAEPLDLA
                     ENLLGALNEERRYALDSEGQQGGRRHGGGR"
BASE COUNT         1060 a         2588 c         2767 g         1089 t
ORIGIN      
        1 agggccgcca ggttgaggtc atgcacgctc agggctacgg cgacgcctcg ggaggcgagc
       61 tccctgagga ggccgaggac ctgcatctgc ctcgcgatgt caagctcggc cgcgggctcg
      121 tcgaggagga ggacggccgg cctctgggcg agggctcgcg cgatgaggac catgcggaac
      181 tccccgcccg agagctcggt caccctgcgc gacgcgaagg gctcgaggcc cgtggccatg
      241 agcgcgtcat cgacagcctt catttcctcc tcgccggggc tgccgaacca gccggtatgg
      301 gcgaaggtcc ccccctcgac gtattcccgc accgtggccg gccatgaggg gctctcggcc
      361 tggaggaccc tggctatggt cctggccctc gccggccggc tgagctcagc ggtctcgacg
      421 aggtcatcct ccgggccggg ggcgacgagg gccgtgccgg ccagggccct gagctcgccc
      481 gagagggtcc tgaacaatgt ggtcttgccc gagccgttgg gccctatcag cgcaaggacc
      541 tccccatccg cgaggtcgag gtcgactccc tcgaggaccc tccggccggc gtagccgcag
      601 gagaggccac gcgcggcgag gcgtaccctc cgatcgctca acgcgcggcc tccctcgggc
      661 cttcgcccgg catgcgccgc gcgaggatgc ggaggaagag cggggccccg acgatggcgg
      721 tcacgatgcc cacgggcagc gcgaggggag aggccaccga gcgcgccacg gcgtcggcga
      781 ccatgaggag ggcggctccg gtgaaggcag agacggggac caggcgcctg tgccccgggc
      841 cgacgaggag gcgggcgagg tgggggccga ggaggccgac gaagccgatg atgccgcccg
      901 ccgagaccgc cgccgccacc ccgaggctcg aggccgcgac cgcgagcctg cgggtcctct
      961 cgatgtccag cccgagtgag gaggccgcct cctccccggc gccgagcagg tcgagggggc
     1021 gggcgagcag ggaggagagc ccgatcgagg ccgcggcgag ggggagggcg gcgccgaggt
     1081 cgggccagct cttccccccg aagccgccga ggagccagaa gaagacgcgc tgcaggtcgc
     1141 ggctgtccag ggtcacgatg agcgacacca atgccccgag gaaggagcca acggcggcgc
     1201 ccgcgaggag gagggcggcg gtctggggct gcctccgcgc cgagcgcgcg atcgcgtaga
     1261 ccatgaaggc cgagccgagg gcccccgcga aggcgaggat ctcggtggcc ccgatcgcca
     1321 cgagtgggga tgagaggccc agggagatcg ccgccacggc cccgagcgag gcgcccgagg
     1381 acacgccgag ggcgtagggg tcggcgaggg gattgcggaa caggccctgc atcaccgcgc
     1441 cggcggcccc gagcgcggcg ccggcgaccc cggcgaggag gctcctcgcc aggcgaaggt
     1501 cccagacgat gaccgtcgcc atcgggtcgg ccgcggtccc agagacggcc gggaggccgg
     1561 gaatgaggcg ggtggcaagg gccgaaagga cctgtcccgg gggcaggggc gaggagccga
     1621 tgcagaggct gacgaggagg gccaggagga aggagagggc ggctatcgcg gcgccgaggc
     1681 gggcgcgatg ggtcctgccc ggcttcatgg gaagagctcg gggtgaaggg ccttggcggc
     1741 ggccatcacg ccagccgcca cccttgggcc ggcacgggag acgagggcag cggggaggag
     1801 gaggatccgg ccgctccgca cggcgggcac ggtggaccag ccgggcctct ggccgatgcg
     1861 cgccgcgctg accttgtccc ccatgtcgtc ggggctcagg atgacctccg gggcccggcg
     1921 gatgacctcc tccgagctca cctggggcca gggacccttc aggtctgcga agatgtcgcg
     1981 tcctcccgca gcctcgagga tggcgtgggg cagggagacc gagccgcagg tcatcagggg
     2041 atcgtcgtag accagccaga gcacccgggg cctctgggcg atggggatgg ccgaggtggc
     2101 gtcggtgatg cgctttatgg cccccgtgag ggcggcggcg gccttcagcg cggcccctgg
     2161 agtcccggac agggctccga gggccatcat cgcgtcggcg acccccctga agtccccggg
     2221 ctcgtaggcg aagaccctga ggccgagcct cctgaggctg gcctcgagac ccgcgtggag
     2281 ggagcccccg ctcacgacga ggtcgggctt cagggccagg accctttcga cgctcacggt
     2341 ctcggcgacg aatcctccca ccttgggcag gctccgcgcc tcctcggggt agtcgcagta
     2401 ggtggtgtcc ccgacgagct tggggcccgc cccgacggcg aaaagggcct cggtggccgc
     2461 gggggagagg gagacgatcc gcgccgcggg cgcgtcgagg ctcaccttcc tgccgaggga
     2521 gtcgactgcg ctgacgccct gggccgcccc gtccgccgcg gacagggcca agaaggcgag
     2581 ggcgacgagg gcgaaagacc cgctccccac aaaggctcgc ctcatgtcgc ggtccctagg
     2641 ctccggagac gccggcctcg tcgaaggtgg ccatctcgtc gaggacgcgg caggcggcgt
     2701 cgcagaccat catcgcaagg gctgcccccg tgccctcgcc gagcctgagg cccaggtcga
     2761 ggacggggtc gagccccagg tcggtcagca cgacgcggtg gccgggctcg atggacaggt
     2821 gggaagcgat catgtactgg gcgcagagcg ggcagaggcc ctgggcgacg agggctcccg
     2881 cccccgagat gtagccgtcg atgaggatgg ggatccggtg ggcggcggcg gcgaggatgg
     2941 agccggcgat ggccccgatc tcgaagccgc cgaccgcggc caggacgccg agggggtcgc
     3001 gggcgtcggg cgcgttgagc gaaagcgccc tgaggatggc cgcggtcttg cgggcaagcc
     3061 cggcgtcgtc gacccccgtg cccctgccca ccacggtggc ggggtcggag ccggtgagga
     3121 cggcggtgat ggcggccgag gccgtcgtgt tgccgatgcc catgtcgccc gtggcggcga
     3181 tgccgaaggg cgagcgctcg agctcggcct cgaagacttc gatcccgacc tgtatggcgg
     3241 ccatggcctg gtcccggctc atcgcgctgc cgcgggccat gttccccgtg cccggcgcga
     3301 ccttccgcga gtagaggttc ggcgcggcag ggatctctga ggccacgccc acgtccacca
     3361 cgaccacccg cgcccctgcg cgcctcgcga ggacgttgat cgccgctccg cccctgagga
     3421 agttctggac catctgcgcg gtgacggcct gggggaaggc gctcacgccc tcggcgcaga
     3481 ccccgtggtc ggcggccatg acgaacacgg cgcccgggcc aagtcggggc cgggtctggc
     3541 ccgtcatgcc cgcgatggca atggagagct cctcgaggcg gccgaggctg ccccctggct
     3601 tggtcaggcg gttctggcgg tcgcgcgcgg cctcggccgc ggcgttgtcg cgcggcttga
     3661 tcgccgcgat ggtcttttcg taggccgtca ttattcagca cccagtgaat aaccggagat
     3721 ggacgcgccc atggcgcgtc tgcgcggcag gtctcctggc tcgaaggctt taccgatgct
     3781 gtccccctcc ttcccgcttt cgcagtggtt ggggaccggt tacgaactgg ccttcctaca
     3841 gtgtctgggg gcgcgccgga atcggaccgg cttcccaatt aagcctttcg gcgccgcgca
     3901 gggcctaaat ctatgacggg ctgacgcggg gggtcaagtg gagcttgacc cctgaaccgg
     3961 ctccgggcta tcaccgaggg cgatgcgcac cctcatcaca ggcggagtga agtcgggcaa
     4021 gagccgccac gccctcgagc tcgccatgga attccccgag ccccgccgct tcctcgccac
     4081 ggctgaggcc ttcgacgacg agatgcgcgg gaagatcgcc cgccacaggc tcgagcgctc
     4141 ggacaatttc gagacaatcg aggagccgat agacatccac gagcggctcg ccgagcgcat
     4201 ggtcctcgac tgcctgccgc tctggctcaa caacctcatc cactacggcc gcgagtccga
     4261 gttcgaggtc atcctctcgg ccctcatcgg gcgcctgccc cgggacatcg tcatcgtgag
     4321 caacgaggtc ggcatgggct tcgtgccggc cgatgccctg agccgccgct atggagtcct
     4381 cctcgggacg gcgaacgcga ggctggcggc cgtctgcgac agcgtcgtcc tcatggtggc
     4441 cggcgtcccg ctcagggtga agtaggccag agccgatgcg cctctgcgtc ccctcctacc
     4501 tggtgcccgg cacctggctc gagaacctcg aggcgatgcg ggacgaggtc tggatcgagg
     4561 gggtcgagct cctcttcttc gcctgggaca gggacgccca agagatcctc gcccgcgagg
     4621 gggaagggat tgccgccctc tcctcgcgct tcgacttcag cctccatctg cccgatcggc
     4681 tcggaccgga ggcgctgagc ctcgttgagg cgacagagtc ctgcgtctcc tcctacatcg
     4741 ctcatccgcc tccctctgcg gacgatgccg gggcctgggc caggctcctc ggagcctggc
     4801 gcgagcgttt cggcgaccgt ttcctcctcg agtacacggg cgccacggcc tttgcccgcg
     4861 gcgaggaggc cctccccggc ctgccgctct gcgccgacac cggcaagctc cttgtggagg
     4921 ggatcgagcc ctcggcctgg attggcccga ggtcagggag gatcggcgag gtccacctcc
     4981 acggggccga ggccgggcgc gaccacaggc ccttcggcgc ggctgcgccc tggctccgga
     5041 ggctcctgcc cttccttgtt ggtttcgagg ggagggtcaa catcgaggtc ttcgagctcg
     5101 aggccgcgcg ctcggccgcc ctggccctcg ctgccgccct cgccggaagg ggcggtccat
     5161 gagggtatgc ctgcgccgat tcctctcggt cttcagcctc gtgtcgcgga tccccgtgcg
     5221 gacggccttc gagtgcgatt acgcgagggc cgacttctgg cttcccatcg tcggcgtctt
     5281 cgcctcggcg gcggccctgg ctggatatgg cctcgcctcg ctggtcttcg cctcgcccct
     5341 gctgcgaggc ctcggcgccc tcttcgtcca gtactcggcc ttcaacctct tccacctgga
     5401 cggcctgctc gacagcgcgg acgcgatgac cccctatgcc tcgcgggaga ggcgccacga
     5461 gatcctcaag gaccccagga tcggctccta tgccctcttc tacggtttct tcgccctcgc
     5521 cgccaagctg gccgccctcg cgtccctggg cccgccggcc atcctcgccc tctttctcgc
     5581 ctacccagcc gcgggccggc tcggggcagc cctcctcccc gccctcatcg gcccggcgcg
     5641 ggagggcggc ctcggggccc tgatgcgggg cctctcgccc ctgcgctcgg cccttggctt
     5701 cgccctggcc ctcatgccgc ttctcgcctg gtcggccgct agcggggcag gtaggaccgg
     5761 cctcgcggca gccgcctgcg ccatcgcggg ggcagccatc ggagccttgt ggatggccct
     5821 cctttatcgg aggaagctcg gcggcttcac cggggacgcc ctgggcgcgg ccgtcgagct
     5881 cggcgagctc gcctgcgtcc tgcttttcgc cgccctcgcc tagatcttct tcgcgtacag
     5941 cggcgaggtg agcagctccc tgtcgtccac ggtgagggtt atgctcaggg tgccctcgtg
     6001 gtcgatgggc gtgttgagca gggtaaagac gctgtggatc acgcgggagg agccggtgaa
     6061 gtccttgacg acggcctcgc cgtatatggt cttgatggcc tcggtgccgt cctgggccac
     6121 gaagacgatc ttgatggcgt gcttcccagc ctcgtggatg tagaagcgca gcctgacggc
     6181 gaggcacatc gcgggatgga cggccgggaa gcggcgcgcg tagatggtgt cgaaggcccc
     6241 gacgatcgtg agcttgccgt cgctctcctg ggcaaagtcg cacaacgtga aaacatcagc
     6301 gttcatcgcc cgcctccatg ccgcctgccg ccctgctggc cttcggaatc gagtgcgtag
     6361 cgcctctcct cgttcagggc cccgaggagg ttctcggcga ggtcgagggg ctcggccctg
     6421 agcctgtggg gcccccggtc gggctccctg aggagggcgt cgaggtcgag gtcctcgggg
     6481 ctgtggactg cgaggccgac cccgaagggg ggctcgtgtc cgcggccgag ggcgtcgagg
     6541 gtcgtcccga gccggagccg cgcgatctcg gcctcctcgg gccccgtcgc ggggaggagc
     6601 acgatgaact cgtccccgcc gtggcgggcg acggaatcca cgtccctgac cgtcttgatg
     6661 agggtctcgg ccacgagggc gagcaccgag tcgcccgccg cccggccgaa ggtctcattg
     6721 atcgccttga agccctcgac gtcggcgagg atgaccgaga actccccccc gtacctcgcc
     6781 gccctcaggg tctcgcggtc cagcgcgagc cgcaggcccc tgcggttacg ggcgccagta
     6841 agggggtcga gctcggagag ccggcgggcc tcggcgaggt agtaggcctt ctcgacggcg
     6901 atggcggcga agtccacgat agtggacagg gccaggaggt cggcggcggc gtagctcctg
     6961 tcctcctggc ggcagtcgag ctcgatcacg ccgaagaccc tgtcctccga gcgcagtggg
     7021 gccagcatcg aggtcctggt ccggaaggcc cctgcccggt cgatgcggtc ggagaagcgg
     7081 gcatcggagg ccgcgtcccc gacgaggagg gggaggccgg ccttggcgac ccaggccgcg
     7141 aggccctcgt cgccggggat ggccatgccg aggagggagt cggcggccgg gcccctgcag
     7201 gaggcgaaat tcagcatgcc ggaatccagg aggaggaggg atgaggcgtg gggaccgaag
     7261 taggagccaa gctcgtccat gaggcggtcc agcacccccg tgatgctctg ttcggccatg
     7321 atcccgctca cgatcgagga gaagaccgaa agccgcacca ggtcgtcggc gccgggctcg
     7381 ccggctttgt cgcgcatgtc cgcctcctca gatactatgg cgcgggccgt ccctggcttc
     7441 aagtcgacgg gacggcgcgc ttgaccattc ccgagcaagg gcctaatgtt tgccccggga
     7501 tgtc
//
LOCUS       DASSQV010000051         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1009551, whole genome shotgun sequence.
ACCESSION   DASSQV010000051 DASSQV010000000
VERSION     DASSQV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009551"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFL04_00920"
     CDS             complement(<1..214)
                     /locus_tag="VFL04_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838289.1"
                     /translation="MDYLQTLEYQMLSLQRKLRLLQELRFFVESSTADELGAFAAELR
                     SVTTVGGISVGEALASALASTSVSAAS"
     gene            314..1501
                     /locus_tag="VFL04_00925"
     CDS             314..1501
                     /locus_tag="VFL04_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838290.1"
                     /translation="MSIDAADLKKLIKIVRQYEVIYGGGISTSDAAVREECEYLVGAL
                     DAELGSSPKSGGLKARRGSLALPSLVEAKPRTRAEKAPRPVSSRTSELVRLTKPDTSV
                     SHAAPEAKGAKAGKGKAEPATAKGKAAPAATKGKAEPAPAKGKAAPAPAKGKAAPAID
                     KGGTTASGSRIHPDIRSRSPDAARAGDHRSDLRAGSARSAKAESSGPDPKRRFAASHL
                     FDAYSQGKLPKYGGFIVCIRFAEELGYSIVEIIGYENLQDIYPDGEGLVFKSVGCKLY
                     AIVEPPNYLLKGVEPVNRPMEQAVPYRFNELLKITTKRFQTLYVGRKPVFMPTSFSVL
                     KPTGEDLAVLFYDIADVYSNIQDFLIMLFRDRMQIPVPDSRKGGEVIARGIREFHLWL
                     DEA"
     gene            1504..2409
                     /locus_tag="VFL04_00930"
     CDS             1504..2409
                     /locus_tag="VFL04_00930"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7838291.1"
                     /translation="MSSSVPLSGTGGDDAALDILLTEFEGGDEAELKAAIGRMGQSAS
                     SGDAVNNGQAKYVFAAAYDEPSLRSHRAAALDERRPWCFCVHHDDRNLVAAASVAREG
                     RMLLLPPDERELKRLFTALGDDYRERSSGDALFSGLDRLEASFRWKTSQIDVSRVCRR
                     VAKILAEAGFYPDRGEEDECALALEEAIVNSTEHGNLELDSSLRPDDLLAEDLYETER
                     QRRMADPAYGDRKVVVGLRITAAEATVDLSDEGKGFDVSKVNEDPSGLEVSGKGFWLI
                     KRPFDAATYNATGTRLTLVKRRPGS"
BASE COUNT          475 a          854 c          794 g          345 t
ORIGIN      
        1 tcgaggccgc cgagacggaa gtcgaggcga gggccgaggc gagggcctcc cccaccgaga
       61 tgccgcccac cgtcgtgacg ctccgaagct cggcggcgaa ggcccccagc tcgtccgcgg
      121 tcgaggactc gacgaagaag cggagctcct ggagaaggcg gagcttcctt tggaggctca
      181 gcatctggta ctcgagggtc tgaaggtagt ccatccttgg gaattctacc cgccgggtcc
      241 gcgagcgtca aaggcaaagc gcatttggcg gtccgaattt gactgtccat ccaatacgtc
      301 tatacttgtc gccatgagca tagacgcagc cgacctcaag aaactcatca agatcgtccg
      361 gcaatacgag gtcatctatg gcggcggcat cagcacgagc gacgcggcgg tccgcgagga
      421 atgcgagtac ctcgtcgggg ccctcgacgc cgagctcgga agctcgccca agtccggtgg
      481 gctcaaggcg aggcggggct cgctcgccct cccctccctg gtcgaggcga agccccggac
      541 ccgcgccgag aaggccccca ggccggtgag ctcccgaacc tcagaactcg taagattgac
      601 gaaacccgac acgagtgtca gccatgcggc ccccgaggcc aagggagcga aggcaggcaa
      661 gggaaaggcc gagcccgcga cagccaaggg caaggccgcc ccagccgcta ctaaaggaaa
      721 ggccgagccg gccccggcca agggaaaggc cgcgccagcc cccgcaaagg gcaaggccgc
      781 tccggccatc gataagggcg gaacgacggc ctcgggctcg cgcatccatc ccgacatcag
      841 gagccgatcc ccggacgccg caagggcggg cgaccaccgc tccgacctca gggcgggctc
      901 ggcccgcagc gccaaggccg agagcagcgg ccccgacccc aaacggcgct tcgccgccag
      961 ccacctcttc gacgcctatt cccagggcaa gctgcccaag tacggcggct tcatcgtctg
     1021 catccgcttc gccgaggagc tcggctactc gatcgtcgag atcatcggct acgagaacct
     1081 ccaggacatc taccccgacg gtgagggcct ggtcttcaag agcgtgggct gcaagctcta
     1141 cgccatcgtg gagccgccga actacctcct gaaaggggtc gagccggtga accggccgat
     1201 ggagcaggcg gtgccctacc gcttcaacga gctcctcaag atcacgacaa agcgcttcca
     1261 gaccctctac gtcggccgca agcctgtctt catgcccacg agcttcagcg tgctcaagcc
     1321 cacgggcgag gacctcgccg tcctcttcta cgacatcgcc gacgtctaca gcaacatcca
     1381 ggacttcctg atcatgctgt tccgggacag gatgcagata ccggtgcccg attccaggaa
     1441 ggggggcgag gtcatcgccc gcggcatcag ggaattccac ctctggctcg acgaggcatg
     1501 atcatgtcca gttcagtccc cctttccggc acgggcggcg atgacgccgc cctcgacatc
     1561 ctcctgaccg agttcgaagg cggcgacgag gccgagctca aggctgccat cggccgcatg
     1621 gggcagagcg cgagctccgg cgacgcggtc aacaacggcc aggccaaata cgtgttcgcc
     1681 gccgcctacg acgagccctc cctgcggagc cacagggcgg cggccctgga cgagaggcgg
     1741 ccctggtgtt tttgcgtcca ccacgacgac aggaacctcg tggcggcggc ctcggtggcc
     1801 cgcgagggcc ggatgctcct ccttccgccg gacgagcgcg agctcaagcg gctcttcacc
     1861 gcccttggag acgactaccg cgagcgcagc tcgggcgacg ccctcttctc cggcctggac
     1921 aggctcgagg ccagcttccg ctggaagacg agccagatcg acgtgtcgcg ggtctgccgc
     1981 cgcgtggcga agatcctcgc cgaggccggc ttctaccccg accggggcga ggaggacgag
     2041 tgcgccctcg ccctcgagga ggccatcgtc aactcgaccg agcacggcaa cctcgagctc
     2101 gactcctccc tccgacccga tgacctcctc gccgaggacc tctacgagac ggagcggcag
     2161 aggcgcatgg ccgaccccgc ctacggcgac cgcaaggtcg tcgtcgggct caggatcacg
     2221 gcggccgagg ccaccgtcga cctcagcgac gagggcaagg gcttcgacgt ctccaaggtg
     2281 aacgaggacc cttccggcct cgaggtctcg gggaagggct tctggctgat caagaggccc
     2341 ttcgatgcgg cgacatacaa cgcgacgggc acgcggctga ccctggtcaa gcgcaggccc
     2401 ggctcgtgaa cgacatacat ggaggtgtta tggattttag cgacagggaa ttgaaagtcg
     2461 tgctacag
//
LOCUS       DASSQV010000052         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2378335, whole genome shotgun sequence.
ACCESSION   DASSQV010000052 DASSQV010000000
VERSION     DASSQV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378335"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFL04_00935"
     CDS             <1..321
                     /locus_tag="VFL04_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838292.1"
                     /translation="TSGISAGRGYVANAAQVVAGGTAVGAIFASLIFGLTDALATFLQ
                     NIGMPPELIQALPYAVTIIGFVLVTLSRDRREAARKRALAADAAAKAAQAQTAAGPGG
                     VSRA"
     gene            321..1652
                     /locus_tag="VFL04_00940"
     CDS             321..1652
                     /locus_tag="VFL04_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7838293.1"
                     /translation="MDCGLLIEGCAVLTMDAEYRIFDPGFVAVRDGAIVGLGPAAEAA
                     HWTAARRIDARGRLLMPGFVNAHTHVAMAAFRGACEDLDDRLVRFIFPMEKSLVSRDL
                     VFESTRFCLAEMARSGTTCFADMYYFEDEVARAADQVGMRCLLGETVVDFPAPDAGEA
                     YGGIAYARRFVEDWRGHPLVSPCFAPHAPYTVDAEHLKLIWAEAESQGVPVLIPLAET
                     EREAARFRASHGSPVKYLESIGCLGSRLVAAHMIFVDAEDIAILAERDVAVAHCPASN
                     AKSGRPIAPAWKYRAAGLRMGLATDGPISGNGMDMMGVTGLYPKLQKVLSGRREEVSA
                     REALRAATLGGAEALGLGHLVGSIEPGKRADLVLADTDDFNVQPVYDWYATAVYALRP
                     HNVRTVLVDGRVIVDEGRMATIDEAEAKAAMRAITSRCRLEIARLTKELLP"
     gene            1649..2386
                     /gene="deoD"
                     /locus_tag="VFL04_00945"
     CDS             1649..2386
                     /gene="deoD"
                     /locus_tag="VFL04_00945"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET7838294.1"
                     /translation="MNDNGNEVLPEGTPHISPEAPIAPTVLMPGDPLRAQYIAEHFLD
                     GARRFNRVRGMLGFTGSYKGMPVSVMGSGMGIPSMGIYSYELFDHFGVQRILRLGTCG
                     GYRPDMELGDIVMAQASSTNSSWASQYGLGGSFSAIPDFGLLEAAVAAARRLGRRFVV
                     GNTLCLDQFSRYHKLPQVWKPWVEMGIVAGDMEAYALYCNAASLGKKALAMFTVSDLR
                     LKDLHMSVEEREKALGSMIEVALESLV"
     gene            complement(2383..>2587)
                     /locus_tag="VFL04_00950"
     CDS             complement(2383..>2587)
                     /locus_tag="VFL04_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838295.1"
                     /translation="TWPLGVGAHTWQSCAASGSSLGFKGMLYAAKVLAGAGLELVRKP
                     AILAAARAEHRKKTSPYRSTMDL"
BASE COUNT          374 a          980 c          838 g          395 t
ORIGIN      
        1 acctcgggga tctcggccgg gcggggctat gtggccaacg cggcccaggt cgtcgccggg
       61 ggcacggccg tcggcgccat cttcgcctcg ctcatcttcg gcctcaccga cgccctcgcc
      121 accttcctcc agaacatcgg gatgcctccc gagctcatcc aggccctgcc ctacgccgtc
      181 accatcatcg gcttcgtcct cgtcaccctc tcgcgcgacc ggcgcgaggc cgcccggaag
      241 cgcgccctgg ccgctgacgc ggccgccaag gccgcccagg cccagaccgc ggccggcccc
      301 ggcggcgtga gccgagcgtg atggactgcg ggctcctcat cgagggctgc gccgtcctga
      361 ccatggacgc cgaataccgg atcttcgatc cgggcttcgt cgccgtgagg gacggggcca
      421 tcgtgggcct tggaccggcg gctgaggccg cgcactggac ggcggccagg cggatcgacg
      481 cgaggggcag gctcctcatg ccgggcttcg tgaacgccca cacccacgtc gcgatggctg
      541 ccttcagggg ggcctgcgag gacctcgacg accgtctcgt ccgcttcatc ttccccatgg
      601 agaagagcct cgtcagccgc gacctcgtct tcgagtccac gcgcttctgc ctagccgaga
      661 tggctcgctc gggcaccacc tgtttcgccg acatgtacta cttcgaggac gaggtggcga
      721 gggccgcgga ccaggtcggt atgcgctgcc tcctcggcga gaccgtcgtc gacttccccg
      781 cccccgacgc gggcgaggcc tacggcggca tcgcctacgc gaggcgcttc gtcgaggact
      841 ggaggggcca tcccctcgtg agcccctgct ttgcccccca cgccccgtac acggtcgacg
      901 ccgagcatct gaagctgatc tgggccgagg ccgagagcca gggtgtcccc gtcctcatcc
      961 ccctcgccga gaccgagagg gaggcggccc gcttccgcgc ctcccacggc tcccccgtga
     1021 agtacctcga gtcgataggc tgcctgggct cccgcctcgt ggccgcccat atgatcttcg
     1081 tcgacgccga ggacatcgcc atcctcgccg agcgcgacgt ggccgtagcc cactgtccgg
     1141 cctccaacgc caagtcgggc aggcccatag cccccgcctg gaaatacaga gccgccgggc
     1201 tccgcatggg cctggccacc gacggcccca tctcgggcaa cggcatggac atgatggggg
     1261 tcacggggct ctaccccaag ctccagaagg tcctctcggg ccggcgcgag gaagtgagcg
     1321 cgcgcgaggc cctgcgagcc gccaccctgg gcggggccga ggccctcggc ctcggccacc
     1381 tcgtcggctc catcgagccg ggaaaacggg ccgacctggt cctcgccgac accgatgact
     1441 tcaacgtcca gcccgtctac gactggtacg ccaccgcagt ctacgccctt cgtccgcaca
     1501 acgtccgcac cgtcctcgtc gacggccggg tcatcgtgga cgagggccgg atggccacca
     1561 tcgacgaggc cgaggccaag gccgcgatgc gcgccatcac ctcgcgctgc cggctcgaga
     1621 tcgccaggct cacgaaggag ctcctgccat gaacgataac gggaacgagg tcctgcccga
     1681 ggggactccc cacatttcgc ccgaggcccc gattgccccg accgtcctca tgccgggcga
     1741 ccccctgcgg gcccagtaca tcgccgagca cttcctcgat ggcgcgcgcc gcttcaaccg
     1801 cgtgcgcggc atgctcggct tcacggggag ctacaagggc atgcccgtct ccgtcatggg
     1861 ctcgggcatg ggcatcccct ccatgggcat ctactcctac gagctcttcg accacttcgg
     1921 cgtccagagg atcctccgcc tgggcacctg cgggggctac cgccccgaca tggagctcgg
     1981 cgacatcgtg atggcccagg cctcctcgac caactcctcc tgggcgagcc agtacggcct
     2041 cggcgggtcc ttctcggcca tccccgactt cggcctcctc gaggcggccg tcgccgccgc
     2101 gcgacgcctc ggcaggcgct tcgtggtcgg caacaccctc tgcctcgacc agttctcgcg
     2161 ctaccacaag ctgccccagg tctggaagcc ctgggtcgag atgggcatcg tggccgggga
     2221 catggaggcc tacgccctct actgcaacgc ggcctccctg ggcaagaagg ccctcgcgat
     2281 gttcacggtc tcggacctga ggctcaagga cctccacatg agcgtcgagg agcgcgagaa
     2341 ggccctcggc tcgatgatcg aggtggcctt ggagtccctc gtctagaggt ccatcgtcga
     2401 gcggtagggc gaggtcttct tcctgtgctc tgccctcgcc gcggcgagga tggccggctt
     2461 ccgcaccagc tcgaggccgg cgcccgcgag gactttggcg gcgtagagca tgcccttgaa
     2521 gccgaggctc gagcccgagg ccgcgcagga ctgccaggtg tgggccccga ccccgagggg
     2581 ccaggtg
//
LOCUS       DASSQV010000053         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2379411, whole genome shotgun sequence.
ACCESSION   DASSQV010000053 DASSQV010000000
VERSION     DASSQV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379411"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFL04_00955"
     CDS             <1..241
                     /locus_tag="VFL04_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838296.1"
                     /translation="AAALAREGADKAPGDGSLGRAAAAFRGNFVATEHNHFAALFNAG
                     DYAGAAAVAKAALARLPGDRTLQTDLDLAGKAQGQ"
     gene            complement(259..1779)
                     /locus_tag="VFL04_00960"
     CDS             complement(259..1779)
                     /locus_tag="VFL04_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET7838297.1"
                     /translation="MERTSGLRVIYAIEDEDERVAFAALAEALKPPFVVHYPADIAEV
                     ERLYSADAADVIVTDFGFHNGTLADWLTLWPLPSVVVIGREDDPARVERTIKDESSVF
                     IERRPEGQHVRLIPALIRKVVNIRESLRRQNAHLQMTEHQYLNLLQAVPDIVYILDGD
                     GRFIYLNDAIRNLGYDPAKLIGRHFSEIIHPADVPKVSKIEVLDGLKGSVTGQEGSPK
                     LFDERRSGRRMTRNLELRLMPGAESADFRYASVNAYGEVNCSGYHLPEYEGQGLGTVG
                     VIRDVSDRKNHQRELEAALTAREVLLKEIHHRVKNNLQIVSSLLNLQEASVTDEASRK
                     VFLECQSQIQSMAMVHEVLYRAGDFKGVDMQKYFERLLEYLAGVYDSSFNGISCEVEA
                     RETTLGLDAAIAVALIVNELVSNCFKHAFPDGRAGRVSLTMSPQDGDWILRVADDGVG
                     FEATRSEDSKGIGTELVEALAAQLRGSVEHGASRGPQGGALVSVRFPREGRPDLPA"
     gene            1895..>2486
                     /locus_tag="VFL04_00965"
     CDS             1895..>2486
                     /locus_tag="VFL04_00965"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET7838298.1"
                     /translation="MSNEHDFTVHALGACSISSPIAFSTQSGDSMPNYVSDEDFVRYE
                     VGAHEGEQPAIERKSLFQKAGPRSHIYFAPSHVHAGIVTCGGLCPGINDVIRAIVRCL
                     WFRYGVRRITGIRYGYRGFLPEYNLGIRPLDPEAVDEIHKVGGTFLGSSRGGGERTQD
                     IVDAIERLNLNVLFTIGGDGTQKGALAIAEEIERRNL"
BASE COUNT          384 a          814 c          892 g          396 t
ORIGIN      
        1 cgccgcggcc ctcgcgaggg agggggcgga caaggccccc ggcgacggga gccttgggcg
       61 ggcggccgcg gccttcaggg gcaacttcgt cgccaccgag cacaaccatt tcgcggccct
      121 cttcaacgct ggggactacg cgggggccgc cgccgtggcc aaggccgcac tcgcccgcct
      181 cccgggcgac aggaccctgc agaccgacct cgacctggcg gggaaggccc agggccagtg
      241 atgccccggg ccgaccggct aggccggtag gtcggggcgg ccctcgcgcg ggaacctgac
      301 cgacacgagg gccccgccct gggggccgcg gctcgccccg tgctccaccg agccgcggag
      361 ctgggcggcg agggcctcga cgagctcggt cccgatgccc ttggaatcct cgctgcgggt
      421 cgcctcgaag ccgaccccgt cgtcggccac cctgaggatc cagtccccgt cctgggggga
      481 catcgtgagc gaaaccctgc ccgcccggcc gtcggggaag gcgtgtttga agcagttgga
      541 gacgagctcg ttcacgatca gggccacggc tatcgcggcg tcgaggccga gggtggtctc
      601 cctggcctcg acctcgcagc tgatgccgtt gaaggaggag tcgtagacgc cggcgaggta
      661 ctcgaggagg cgctcgaagt acttctgcat gtccacgccc ttgaagtcgc cggcccggta
      721 gaggacctcg tgcaccatgg ccatggactg gatctgggat tggcactcga ggaagacctt
      781 gcgcgaggcc tcgtcggtca ccgaggcctc ctggaggttc aggaggctcg agacgatctg
      841 gaggttgttc ttgaccctgt ggtggatctc cttgagcagg acctcgcggg cggtgagggc
      901 ggcctcgagc tcgcgctggt ggttcttgcg atcgctcacg tcgcggatca cgccgaccgt
      961 cccgaggccc tggccctcgt actcggggag gtggtagccc gagcagttca cctcgccgta
     1021 ggcgttcacc gaggcgtatc ggaagtcggc gctctcggcg cccggcatga gcctgagctc
     1081 gaggttgcgg gtcatcctgc ggccggagcg gcgctcgtcg aagagcttgg gcgagccctc
     1141 ctggcccgtg acgctcccct tgagcccgtc gaggacctcg atcttggaga ccttggggac
     1201 gtcggccggg tggatgatct cgctgaagtg tctgccgatg agcttggccg ggtcgtaacc
     1261 gaggttgcgg atggcgtcgt tgaggtagat gaagcgcccg tccccgtcga ggatgtagac
     1321 gatgtccggc acggcctgaa ggaggttcag gtactggtgc tcggtcatct ggaggtgggc
     1381 gttctggcgc ctgaggctct ccctgatgtt gacgaccttg cggatgaggg ccgggatgag
     1441 gcggacgtgc tggccctcgg gcctgcgctc gatgaagacc gaggactcgt ccttgatggt
     1501 ccgctcgacc ctggcggggt cgtcctccct tccgatgacg acgaccgagg gcaggggcca
     1561 gagggtgagc cagtcggcga gggtgccgtt gtggaagccg aagtcggtga cgatgacgtc
     1621 ggcggcgtcg gcggagtaga gccgctcgac ttcggcgatg tccgcgggat agtggacgac
     1681 gaagggaggc ttcagggcct cggcaagggc ggcgaaggcg acgcgctcgt cctcgtcttc
     1741 gatggcgtag atgacgcgaa ggccgctggt cctctccatg cttcggcgaa ttgtattggc
     1801 gacagcgagc cgccgtcaag tgcggagccg gggcccggcc atccggggcc ccgaagattg
     1861 catgaggcac ccgcctcgtc tatactaggt cgggatgtcc aacgaacacg acttcaccgt
     1921 ccacgccctc ggggcctgca gcatctcctc gcccatagcc ttctccaccc aaagcggcga
     1981 ctccatgccc aactacgtga gcgacgagga cttcgtccgc tacgaggtgg gcgcccacga
     2041 gggcgagcag ccggcgatcg agaggaagag cctcttccag aaggccgggc cccgcagcca
     2101 catctacttc gcgccgagcc acgtccacgc cggcatcgtc acctgcggcg ggctctgccc
     2161 gggcatcaac gacgtcatca gggcgatcgt gcgctgcctc tggttccgct acggggtgcg
     2221 caggatcacc ggcatccgct acggctaccg gggcttcctg cccgagtaca acctcgggat
     2281 ccgccccctc gaccccgagg ccgtggacga gatccacaag gtcggcggca ccttcctcgg
     2341 ctcatcgagg ggcgggggcg agaggaccca ggacatcgtc gacgcgatcg agcggctcaa
     2401 cctcaacgtc ctcttcacga tcggggggga cggcacccag aagggggccc tcgcgatcgc
     2461 cgaggagatc gagcgcagga acctca
//
LOCUS       DASSQV010000054         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1881158, whole genome shotgun sequence.
ACCESSION   DASSQV010000054 DASSQV010000000
VERSION     DASSQV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881158"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFL04_00970"
     CDS             <1..496
                     /locus_tag="VFL04_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET7838299.1"
                     /translation="LGGGHVGMAIADIAVGLGFSTTVVDDREEMADPTRFPRGVRAVH
                     AAAGYAEAIAGLSLDAASYAVVVTRGHAYDLECVRALLKRSYRYLGMIGSSRKTRMIL
                     EQAKAEGFDPAKVDALFAPVGLDIAAETPQEIAVCILGEIIAVRRNAKVLPELAARRL
                     ARRM"
     gene            560..1888
                     /locus_tag="VFL04_00975"
     CDS             560..1888
                     /locus_tag="VFL04_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7838300.1"
                     /translation="MTGPSAAPGWRAAALARAALARGRAYASGFPLPIWFLTLASTVE
                     STGRFMVVPYLSLFLHGKGVSLGILGLVLASAPVASVLFGAVGGHLSDRWGRKPVQIL
                     GVALSGSALIGFAFAGSDPLLLGLLNFLNGMTRTFYRPATSAAISDFCPSERRSEAFA
                     LNRIAINAAFGWGPLIGVALFATSPKAGFFIAGAVNLCVGLFLALVVPESHAPGRRSE
                     APAAGREAAGAGWSGWREVLTDRGFWLWNLGMMCVWGAYDLIQSFLPLHLVAKGIPLV
                     SYGMLLTTNAAVCVFGQLPVSRAVRVSGIGPITFWSKAGYALGFVGFAFFRSPWALFA
                     SMLVLSLGEILGSGVQSRYIPERAPPRLLGRYMGLATMNELGRALVAPAAGFIMQAYG
                     GELVFVCAGLLSLIGGGLAWKGAAAPAGRPRGDAGPAKARRSPRKIHSGS"
     gene            1938..2684
                     /locus_tag="VFL04_00980"
     CDS             1938..2684
                     /locus_tag="VFL04_00980"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7838301.1"
                     /translation="MDYTVKRLATMAGVSVRTLHHYDAIGLLSPGGRSPAGYRLYGEA
                     ELMRLQQILFFRELEIPLEEIKAVLDRPDFDALTALEGHRRLLAERAGRIARLIDTVD
                     RTIGRIKGETMLTDEELYEGFAPERVQEIKAEAEARWGGTQAWAESRKRMSAMTKESW
                     AAVKAEGAAIDEAILAAMKAGLAPGSAEVQALMGRKFAHLRNFYEPTREMFAGLGRLY
                     CEEGGFRSHYDAMGPGLCEYLREAMEVFAR"
BASE COUNT          441 a         1036 c          966 g          498 t
ORIGIN      
        1 cctcggcggc ggccacgtgg gcatggccat cgccgacatc gcggtgggcc tcggcttctc
       61 caccaccgtc gtggacgaca gggaagagat ggccgacccc acgcgcttcc ccaggggggt
      121 aagggccgtc cacgccgcgg cgggctatgc cgaggccatc gcggggctga gcctcgacgc
      181 cgccagctac gccgtcgtcg tgaccagggg ccatgcctac gacctggaat gcgtgcgcgc
      241 cctcctcaag aggagctacc gctatctcgg catgatcggc tcctcgcgga agacgaggat
      301 gatccttgaa caagccaagg ccgagggctt cgaccccgcg aaggttgatg cccttttcgc
      361 tcccgtcggc ctcgacatcg ccgcggagac gccccaggag atcgcggtct gcatcctggg
      421 cgagatcata gcggtgagga ggaacgcgaa ggtcctgccc gagctggcgg cgcggaggct
      481 cgccaggagg atgtagcgca catcgcgccc tccggggtcc ttaactccat gggccctttc
      541 ccgatatgct tcggccacca tgacaggacc atcagccgcg ccggggtgga gagccgcggc
      601 cctcgctagg gcggccctcg cccgcggccg cgcctacgcc tcgggctttc cccttcccat
      661 ctggttcctc accctcgcga gcacggtcga gtccacgggc cgcttcatgg tcgtgcccta
      721 tctgagcctc ttcctccacg gcaagggcgt ctcgctcggg atcctcggcc tcgtgctcgc
      781 ctcggcgcca gtggcgtcag tcctcttcgg agccgtcggc ggccacctct cggacaggtg
      841 gggcaggaag ccggtccaga tcctcggcgt ggccctcagc ggctcggccc tcatcggctt
      901 cgccttcgcg ggctccgacc ccctcctgct tggcctgctc aacttcctca acggcatgac
      961 ccggaccttc taccgcccgg ccacgagcgc ggcgatctcc gatttctgcc cgagcgagcg
     1021 caggtccgag gccttcgccc tcaacaggat cgccatcaac gccgccttcg gctgggggcc
     1081 cctcatcggc gtggcccttt tcgccacctc ccccaaggcg ggctttttca tcgcgggcgc
     1141 cgtcaacctc tgcgtcggcc tcttccttgc cctcgtcgtt cccgagagcc acgcccccgg
     1201 acggcggagc gaggccccgg cggccggacg ggaggcggct ggggccggct ggtcggggtg
     1261 gagggaggtc ctcacggacc gcggcttctg gctctggaat ctggggatga tgtgcgtctg
     1321 gggcgcctac gacctcatcc agtccttcct ccccctccac ctcgtggcga aggggatccc
     1381 cctcgtctcc tacgggatgc tcctcaccac caacgccgcg gtctgcgtct tcggccagct
     1441 gcccgtgagc cgggccgtca gggtctcggg gatcggaccc atcaccttct ggtccaaggc
     1501 gggctacgcc ctcggcttcg tgggcttcgc cttcttccgc tccccctggg ccctcttcgc
     1561 ctcgatgctg gtcctctccc tcggcgagat cctcggctcg ggggtgcagt cgcgctacat
     1621 ccctgagcgg gcccctccgc ggctgctcgg gaggtacatg ggccttgcca caatgaacga
     1681 gctcggccgg gccctcgtcg ccccggcggc cggtttcatc atgcaggcct acgggggcga
     1741 gctcgtcttc gtctgcgccg gcctcctctc cctcatcggc ggaggcctcg cttggaaggg
     1801 ggccgcagcc cccgcaggga ggccccgggg cgacgcggga ccggcgaagg cgcgccgcag
     1861 ccccagaaaa atccattccg ggtcttgacc ctcacgcaag gggatagcct agctttgcca
     1921 ttaggaggca gctgggcatg gactacacgg taaagcggct cgcgaccatg gcgggcgtca
     1981 gcgtcaggac gcttcaccac tacgacgcca taggcctgct ctctcccggc ggcaggagcc
     2041 cggcaggcta tcgcctctac ggcgaagccg agctcatgcg gctccagcag atcctcttct
     2101 tccgggagct cgagatcccc ctcgaagaga tcaaggccgt cctcgacaga ccagattttg
     2161 atgccctcac agcgctcgag ggccacaggc gccttctggc cgaacgcgcc gggcgcatcg
     2221 cccgcctcat cgatacggtc gatcgcacga tcggccgcat caagggagag accatgctta
     2281 ccgatgaaga gctctacgag ggcttcgccc ccgaacgggt ccaggagatc aaggccgagg
     2341 ccgaggcgcg ctggggaggg acccaggcct gggccgagag ccgaaaaagg atgtcggcca
     2401 tgaccaagga gagctgggcc gctgtcaagg ctgagggggc cgcgatcgac gaggccatac
     2461 ttgcggcgat gaaggccggg ctcgcgcccg gctcggccga ggtccaggcg ctcatggggc
     2521 gtaagttcgc ccacctgagg aatttctacg agccgacgcg ggagatgttc gcaggtctcg
     2581 gccggctcta ctgcgaggag ggaggcttcc gctcccacta cgatgcaatg ggccccggac
     2641 tctgcgaata cctgcgcgag gccatggagg tttttgcgcg atgagcccag gcgggccaaa
     2701 aggatggtga agctttaccc cgaggcggcg atggtctagg atattgggat gtcgctgtct
     2761 tgggggagct tttttcggtg aagacccgca gccctttggc gcagatcgcg aagaagagcc
     2821 tttacgaatc gctcaattcg gacatgctcg agcgggttgc ccgggccgcc ttcccggact
     2881 acagcctcag caagaggacg ggtttccccg agaacatccc cgtgcccgga caggtggcgg
     2941 c
//
LOCUS       DASSQV010000055         5709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2006200, whole genome shotgun sequence.
ACCESSION   DASSQV010000055 DASSQV010000000
VERSION     DASSQV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006200"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VFL04_00985"
     CDS             <1..1222
                     /locus_tag="VFL04_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7838302.1"
                     /translation="QAEREGSASLVYRQRTKAGDYRWISNMVTVIKDAGGRPLYRVGS
                     VRDITEQKRAEAALIEAEERARRRAEDLEAIMDAMPSIILIAHDPACQYMTSSRAANK
                     LLGVPQGSNISLTAPVELHTTSYRLSMGGRELPPEERPMERAAARGESFHNCELCLRF
                     ADGSEAIVYGDVVPLLDAEGRPRGAVGSFTDITEKVRTERRLREAIQATQMLLGELKH
                     RAKNSFAQIQSLISLTTLSTESGEVRAAFADLEARVTAISELYAILHGSESLTLLRLD
                     EYLSRVAVALVQTTRGVALKTRMEALSCSPALAAPLGFILTELLTNAIKYAFPGGREG
                     ELSVSLRKAGAGAVLEVGDDGVGLPRGFDPAGPDSGSGLLLVRSLADQAKASFSIAEG
                     TKGTRCRVEFPLEA"
     gene            complement(1254..2393)
                     /locus_tag="VFL04_00990"
     CDS             complement(1254..2393)
                     /locus_tag="VFL04_00990"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET7838303.1"
                     /translation="MRRREDDRFYDTIALIVDSLGSLGKLDKHAFLKQVFEAAFELIP
                     EAQKGSLYELQGEMFRPVFSRGYDFAVLSRISFGRDEAFIDFDVGSGSSVVARETRIA
                     ARDPAAFPRETLEAFAELGTLSGFTSLYAPICVEGQVAGLISLDKFDDTGFQGLSRKI
                     LQYYARLISQFYAQKLLQERETRRYKDIVSSLVSAIQVMDSHTEGHAQRVRDYSVALA
                     RGLGLGEEEVEEIGTAALLHDIGKLGIPTQILRKPGRLSAEEYEQVKLHPGDGYRILS
                     SIEGFERIAEMAYHHHERYDGKGYPEGLSGEEISLGAQLIGLADAFDAMTSERAYRPA
                     MTVEAALDVIREESGAQFGPRAAAVALGVIADLADRRRELQLQAR"
     gene            complement(2481..3995)
                     /locus_tag="VFL04_00995"
     CDS             complement(2481..3995)
                     /locus_tag="VFL04_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7838304.1"
                     /translation="MAAADSKTKNLATRFILMCVLVLTLVLAMVGAYQYLRSRSSLSS
                     SLALSVKGAGSRLSLALLGPVWDMNTEQAMLALNSEMGNEAIQALSVRPEKEGPLFAG
                     LVRQAQDRVKLADEKDLPPGLMSVVQPIEKDGKRIGDVVVWYTDRGLRSSLAALLLQN
                     VVQAVLVDVILSLFIASLLSRLVTRPLNSLSLFVARLSGGDLDARIDGGLTARNDELG
                     HLGKSVDELRSSIGSVVSQISVSSGRLASGSDALSDTAQGLAQGSNEQAASIEELSAS
                     IEELVSTVRQNAENTHEADSLSSRVAKNAAESGKAVGATAANMNEIASKISIIEEIAR
                     QTNLLALNAAIEAARAGEVGKGFAVVASEVRKLAERSASAAGDINELSRKSVLVASEA
                     GKKLDELVPDVQKTAELIAAIAAASREQSAGADQISKGVGQMDTVVQKNAAISEQLAS
                     TSEELAGQAAELTETIAFFKMGGQEAAAAAAAAPVARRQTTAIAPLGEAELEEL"
     gene            complement(4062..4811)
                     /locus_tag="VFL04_01000"
     CDS             complement(4062..4811)
                     /locus_tag="VFL04_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7838305.1"
                     /translation="MKKLLLSIAFVALAAALFAAPTTLAIGWTEYPPFQISDKAGLDV
                     ELAQAVFTQAGYTLDFQHLPWARQLGNLEAGTLNVVLSASKNAERAKYAEWSSGYRQE
                     RNDLLTLVTSKATVTSLKSLIGTKVKIGAQRGSFYGAEFETLAKDPGFAALLELVPDP
                     ATSLNKMEAGRLDFYVDDIVAATYAIKTNKLPTQVKVALKINGDDVYFMIGKKTLAAD
                     PALMDKVNKAIATLSANGTFTTIYAKYGIVK"
     gene            5144..>5709
                     /locus_tag="VFL04_01005"
     CDS             5144..>5709
                     /locus_tag="VFL04_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838306.1"
                     /translation="MTEHSIAGLAAALALVDTAGVSVALFAALRLLGRARRGGKAEGL
                     LALLLIILSANVAHGALLPRPGSGAPLLLEPLQFLLPVGVAWHLRALRGRRVLEALDL
                     VYLLLALAFAAASFVPALLGPRLPSGLPVFSLVMWLALALSAFLLLFPLARDLHRYRA
                     ELEARFSSLGGLDAAWLRSLLVLTAALFA"
BASE COUNT          819 a         1954 c         1994 g          942 t
ORIGIN      
        1 gcaggccgag cgcgagggga gcgccagcct ggtctaccgc cagcggacca aggcggggga
       61 ctaccgctgg atctccaaca tggtcaccgt catcaaggac gccgggggcc ggcccctgta
      121 ccgggtcggg agcgtcaggg acatcaccga gcagaagcgc gccgaggccg ccctcatcga
      181 ggccgaggag cgggcccgga ggcgggccga ggacctcgag gcgatcatgg acgccatgcc
      241 ctcgatcatc ctcatcgccc acgatccggc ctgccagtac atgacctcga gccgcgccgc
      301 gaacaagctc ctcggggtgc cccagggcag caacatctcc ctcacggccc ccgtcgagct
      361 gcacaccacg tcctatcgcc tgagcatggg cggccgggag ctcccacccg aagagcggcc
      421 catggaacgg gcggccgcga ggggcgagag cttccacaac tgcgagctct gcctccgctt
      481 cgccgacggg agcgaggcga tcgtctacgg cgacgtcgtg cccctcctcg acgccgaggg
      541 caggccgagg ggggccgtcg gctccttcac cgacatcacc gaaaaggtga ggaccgagcg
      601 gcggctcagg gaggccatcc aggccaccca gatgctgctg ggcgagctca agcaccgggc
      661 caagaacagc ttcgcccaga tccagagcct gatcagcctc acgacgctct cgaccgagtc
      721 gggcgaggtg agggcggcct tcgccgacct cgaggcccgg gtcaccgcca tctccgagct
      781 ctacgccatc ctccacggct cggagagcct gaccctcctc aggctcgacg aatacctctc
      841 gcgcgtggcc gtggccctgg tccaaaccac gcggggggtg gccctcaaga cgaggatgga
      901 ggccctgagc tgctcccccg ccctcgcggc ccccctcggc ttcatcctca ccgagctcct
      961 caccaacgcc atcaaatacg ccttccccgg gggcagggag ggcgagctgt cggtgagcct
     1021 ccgtaaggcg ggcgcggggg ccgtcctcga agtcggcgac gacggggtcg gcctccctcg
     1081 gggcttcgat cccgccggcc ctgactcggg atcgggcctg ctcttggtca ggagcctcgc
     1141 cgaccaggcc aaggccagct tctcgatcgc cgaggggacc aagggcacgc gttgcagggt
     1201 cgagttcccc ctcgaggcct aaaggcccat cctggtccgg gagtcgtggc tgcctagcgc
     1261 gcctgaagct ggagctctct gcgccgatcc gccagatcgg cgatgacccc gagggcgacc
     1321 gccgcggccc gggggccgaa ctgggcgcct gactcctcgc ggatcacgtc gagagcggcc
     1381 tcgaccgtca tggcagggcg gtaggcgcgc tcgctcgtca tggcgtcgaa ggcgtcggcg
     1441 aggccgatca gctgggcccc gagcgagatc tcctcgcccg agaggccctc ggggtagccc
     1501 tttccgtcgt agcgctcgtg gtggtggtag gccatctcgg cgatgcgctc gaagccctcg
     1561 atggagctga ggatgcggta gccgtcgccg gggtggagct tcacctgttc gtactcctcg
     1621 gcgctgagcc tgccgggttt gcgcaggatc tgcgtgggta tgccgagctt cccgatgtcg
     1681 tggaggaggg cggccgtgcc gatctcctcc acctcctcct cgcccaggcc caggccccgc
     1741 gcgagggcca cgctgtagtc gcgcacgcgc tgggcgtggc cctcggtgtg gctgtccatg
     1801 acctggatcg ccgacacgag ggagctcacg atgtccttgt agcgtcgggt ctcgcgctcc
     1861 tgcaggagct tctgcgcgta gaactgcgag atgagccggg cgtagtactg gaggatcttg
     1921 cgcgagaggc cctggaagcc cgtgtcgtcg aacttgtcga ggctgatgag gccggccacc
     1981 tggccctcga cgcagatggg cgcgtagagc gaggtgaagc ccgagagggt gccgagctcg
     2041 gcgaaggcct cgagggtctc gcgcgggaag gcggcggggt cgcgggcggc gatgcgggtc
     2101 tcccgggcca cgacgctcga gcccgagccg acgtcgaagt cgatgaaggc ctcgtcccgt
     2161 ccgaagctga tgcgcgagag cacggcgaag tcgtagccgc ggctgaagac gggccggaac
     2221 atctcgccct ggagctcgta gagcgagccc ttctgcgcct cgggaatcag ctcgaaggcc
     2281 gcctcgaaga cctgcttcag gaacgcgtgc ttgtcgagct tcccgaggct gccaagggag
     2341 tcgacgatga gcgcgatcgt gtcgtagaac ctgtcgtcct cgcggcgccg catgtcagcc
     2401 acaagccaca cctttccccg ggccttccgc cccgaaaggc acaaggttcc accgggggag
     2461 gcgggattgt caagccgcgc ctagagctcc tctagctcgg cctccccgag cggggcgatc
     2521 gccgtcgtct ggcgtcgggc gactggggcg gccgccgcgg ccgccgccgc ttcctggccc
     2581 cccatcttga agaaggcgat ggtctcggtg agctcggccg cctggcccgc cagctcctcc
     2641 gaggtcgagg cgagctgctc cgagatggcg gcgttcttct ggaccaccgt gtccatctgg
     2701 cccacgccct tcgagatctg gtccgccccg gccgactgct cgcgcgaggc cgcggcgatg
     2761 gccgcgatga gctcggccgt cttctggacg tcgggcacga gctcgtcgag cttcttgccc
     2821 gcctcgctcg ccacgaggac gctcttgcgc gacagctcgt tgatgtcccc ggccgcgctc
     2881 gccgagcgct cggccagctt gcgcacctcg ctcgccacga cggcgaagcc cttgccgacc
     2941 tcgccggcgc gggcggcctc gatggccgcg ttgagcgcga ggaggttggt ctgccgcgcg
     3001 atctcctcga tgatcgagat cttggacgcg atctcgttca tgtttgccgc cgtggccccg
     3061 acggccttcc ccgactcggc ggcgttcttg gccacccggc tcgagaggga atcggcctcg
     3121 tgggtgttct cggcgttctg gcgcaccgtg gacacgagct cctcgatcga ggccgagagc
     3181 tcctcgatgc tggcggcttg ctcgttcgag ccctgggcca ggccctgggc cgtgtccgag
     3241 agggcgtccg agccgctcgc cagccgtccc gacgataccg agatctggct caccacggag
     3301 ccgatactcg agcgcagctc gtccacggac ttgccgagat ggcccagctc gtcgttccgc
     3361 gcggtgagcc ctccgtcgat ccgcgcgtcg aggtcgccgc ccgagagtct cgccacgaaa
     3421 agcgagaggc tgttgagcgg ccgcgtaacg aggcgggaga ggagggaggc gatgaagagg
     3481 ctcaggatga cgtcgacgag gacggcctgg acgacgttct gcaggagcag ggccgcgagg
     3541 gaggagcgca ggcccctgtc cgtgtaccag actaccacat cgccgatcct cttgccgtcc
     3601 ttctcgatcg gctggaccac gctcatgagg ccgggaggca ggtccttctc gtcggcaagc
     3661 ttcacgcggt cctgagcctg cctcacgagg ccggcgaaga ggggcccctc cttctcggga
     3721 cggaccgaga gggcctggat ggcctcgttt cccatctcgg agttgagggc gagcatcgcc
     3781 tgctccgtgt tcatgtccca gaccgggcca aggagggcca gggagagcct tgagcccgcg
     3841 cccttgaccg agagggccag gctcgatgag aggctcgacc ggctccgcag gtactggtag
     3901 gcgcccacca tggccaggac cagggtgagc acgaggacgc acatgaggat gaaacgggtg
     3961 gcgaggttct tcgtcttgga gtctgctgcg gccatgaggc cctcccgggt gattattgaa
     4021 tgcggggccc ccccgcggag gaggggcccc ggcgctggct cttactttac gatcccgtac
     4081 ttggcgtaga tcgtggtgaa ggtgccgttg gccgacaggg tggcgatggc cttgttgacc
     4141 ttgtccatga gggcggggtc ggccgcgagg gtcttcttcc cgatcatgaa gtacacgtcg
     4201 tcgccgttga tcttgagcgc gaccttgacc tgggtgggca gcttgttggt cttgatcgcg
     4261 taggtggcgg cgacgatgtc gtcgacgtag aagtcgaggc ggccggcctc catcttattg
     4321 aggctggtgg ccgggtcggg gacgagctcg aggagggcgg cgaagcccgg gtccttggcg
     4381 agggtctcga actcggcgcc gtagaaagag ccgcgctgcg cgccgatctt caccttcgtg
     4441 ccgatcaggg acttgaggct cgtgacggtg gccttgctgg tgacgagggt aaggaggtcg
     4501 ttgcgctcct ggcggtagcc cgaggaccac tcggcgtact tcgcgcgctc ggcgttcttg
     4561 gaggcggaga ggacgacgtt gagcgtaccc gcctcgaggt tgccgagctg gcgggcccag
     4621 ggcaggtgct ggaaatcgag ggtgtagccg gcctgggtga acacggcctg ggccagctcg
     4681 acgtccaggc ccgccttgtc gctgatctgg aagggcgggt actccgtcca gccgatggcg
     4741 agggtggtgg gggcggcgaa cagggcggcg gccagggcga cgaacgcgat cgagagcaga
     4801 agcttcttca tgatccctcc ttggagatgt gttgagatta ttcggacccg cgttccttca
     4861 gccgcgggat ccctgccggc ggaaggatcc ccctccccgg ctcgatgccg atgcgttggc
     4921 ccgccccccc ttcggggcgg agcctgagat gcctacatgg attgactttc taaaatgcgc
     4981 tccgaaacga tagccggctt ttacatggct actggttgga gcaactatcg ccaatgtttc
     5041 cagtgctgtc aatacgcaat tgcccgaatc cggacatcgc gcgcggtgag cggcccgagc
     5101 ccgacggggg cgcgaaaacg ccaggccgtg gtagagtgca ggcgtgaccg agcacagcat
     5161 cgcgggcctc gccgccgccc tcgccctcgt cgacacggcc ggagtgtccg tggcgctctt
     5221 cgcggccctg cgcctccttg ggcgggcgcg ccggggcggg aaggccgagg gcctccttgc
     5281 cctcctcctt atcatccttt cggccaacgt ggcccatggg gccctcctgc cccggccggg
     5341 gagcggggcc cccctcctcc tcgagcccct ccagttcctc ctccccgtgg gcgtggcctg
     5401 gcacctgcgg gcgctcagag gcaggcgggt cctcgaggcc ctcgacctcg tctacctcct
     5461 cctggccctg gccttcgcgg ccgcgagctt cgtgccggcc cttctggggc cccggctccc
     5521 gagcggcctg ccggtcttct ccctggtcat gtggctcgcc ctcgccctct cggccttcct
     5581 cctcctcttc cccctggccc gggacctcca ccgctaccgc gccgagctcg aggcccggtt
     5641 ctcgagcctc gggggcctcg acgcggcctg gctcaggagc ctccttgtcc tgacggcggc
     5701 cctcttcgc
//
LOCUS       DASSQV010000056         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_514178, whole genome shotgun sequence.
ACCESSION   DASSQV010000056 DASSQV010000000
VERSION     DASSQV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514178"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1330)
                     /locus_tag="VFL04_01010"
     CDS             complement(179..1330)
                     /locus_tag="VFL04_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HET7838307.1"
                     /translation="MPRAFALIFALACLSLLPRAQASGEMLKSETGFYVDMPAGFTLI
                     DGDGKTRFAFQDPNGGMEFDIRIYDSGRYADSAAMAQDVATKLKSEGEVEGFGYEGRK
                     ATMAELRFLLNGQSMRGYGMFLGGAKTEKSFALLAYCAEPKFEAYADFILSCLDAFSF
                     DRAALRDPGPISQYLLAWPPERKGAKQVRLPGDITVGLPWSPEEAQAETDTAGREYRV
                     LTTYAQSDTLWLDAWSRFYRMVYRESAARLDRLAVEISRGLPQADPTESARRVLAWVQ
                     GFVYERDTKGIDFVTPLVGAYEGRGDCDSRAVVMAAILERLGIDCVLMISREYSHAML
                     AVDVPGGGQRFPWRGKQYLVAETPAKVGLGMIAQTQTDWSKWLGVDLGQ"
     gene            complement(1426..1770)
                     /locus_tag="VFL04_01015"
     CDS             complement(1426..1770)
                     /locus_tag="VFL04_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838308.1"
                     /translation="MEAIRDMSWMRHLALGKPEHFELGGYRVALVSVEATAAHHGDRF
                     RYRLLAFDPGLGKPFLSVDLESDILGDYCLSVEFQGSRHVLERFDSPPALGEFRERAV
                     AEALSRLPGKPA"
     gene            2192..3430
                     /locus_tag="VFL04_01020"
     CDS             2192..3430
                     /locus_tag="VFL04_01020"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7838309.1"
                     /translation="MLSTTTFYLSRMLGATAIGPDDRRVGRVRDIVVDLSQARPRVLG
                     ILAGSGRSERLLPFEECLPTGGSRLRAVRTGPAGEVRLPSENLIYLRKHLLDKQIVDV
                     DGRKLVRVNDLVIDTTSAGAYLVAVDVGAEGLLRRLGIEGPIAALLRVFGTTIPSKLV
                     LWGEVEAVDLPSTQIKLSKAYSKLDGIHPSDLADILEDLDRGSQVAIFTAMDEERAAD
                     VLEELEPEAQVKLLENLSEEKAADVLEKMPADEVADILDELRTDKAERLLAEMEAESS
                     TEVRNLMAYPEGTVGSLMSKDYVFFNQSMKVADALAELRAIKPEAEVVYSLFVLDARG
                     KYVATLSLRDLVVAEPEARLGQIMSSVADPLHDDDAADVLPELMIKYKLHSAPVVDAE
                     GRMAGTIVIEDVLERVMRKR"
BASE COUNT          565 a         1142 c         1218 g          545 t
ORIGIN      
        1 tgccatcgtt cgcggcggcc gggaccgcgg gcgcggcgat ggggctcggc tggttctcgg
       61 tggcgaagga cggggccacg gcgagggccg cggcgagggc gatgagtgct ctagtggtgg
      121 gcagcatggt ctgggactct acaggattat ccgggtaagg gtcaacgcga gggggctctc
      181 actgccccag gtccaccccg agccacttgc tccagtcagt ctgggtctgg gctatcatgc
      241 ccaggccgac cttggccggg gtctcggcga cgaggtactg cttgccccgc caggggaaac
      301 gctggcctcc gcccgggacg tccaccgcca gcatggcatg ggagtactcg cgcgagatca
      361 tgaggacgca gtcgatgccc aggcgctcaa ggatggccgc catgacgacc gcgcgggagt
      421 cgcagtcccc ccggccctca taggcgccga cgaggggggt cacgaagtcg atgcccttgg
      481 tgtcgcgctc gtagacgaag ccctggaccc aggcgaggac ccgccgggcc gactcggtgg
      541 ggtcggcctg gggcaggccc cgggatatct cgacggcgag cctgtccagc cgggcggccg
      601 attcgcggta gaccatgcgg tagaagcgcg accaggcgtc cagccagagg gtgtcgctct
      661 gggcgtaggt ggtgaggacg cggtattcgc gtcccgccgt gtcggtctcg gcctgagcct
      721 cctcggggct ccagggcagg cccacggtga tgtcgccggg gagcctcacc tgtttcgcgc
      781 ccttccgctc ggggggccag gcgaggaggt actggctgat cggaccgggg tcgcggaggg
      841 cggcgcggtc gaaggagaag gcgtcgagac aggagagtat gaagtcggcg taggcctcga
      901 acttgggctc agcgcagtag gcgaggaggg cgaaggactt ctcagtcttg gctccgccga
      961 ggaacatccc gtagccgcgc atcgactggc cgttgaggag gaagcgcagc tcggccatgg
     1021 tcgccttcct gccctcgtag ccgaagccct cgacctcgcc ctcggacttg agcttggtcg
     1081 cgacgtcctg ggccatggcg gcggaatcgg cgtagcgccc gctgtcatag atgcggatgt
     1141 cgaactccat gccgccgttc gggtcctgga aggcgaagcg ggtcttgccg tccccgtcga
     1201 tgagggtgaa gccggcgggc atgtcgacgt agaagcccgt ctcggacttg agcatctcgc
     1261 ccgaggcctg ggcccgcggc aggaggctga ggcaggcgag agcaaatatg agcgcaaatg
     1321 ctcggggcat cggatactcc ttcggaaggg gcatgcccac attatcggcc tgggatcagc
     1381 cgacatgacg ggcggcgcct cggccacggc ggcgccgacc ctggtctagg ccggcttccc
     1441 gggcagcctg gacagggcct cggcgacggc ccgctcgcgg aactcgccca gggccggcgg
     1501 actgtcgaag cgctcaagga catgtctcga gccctggaac tcgacgctga ggcagtagtc
     1561 gccgagtatg tcgctctcga ggtcgacgga gaggaagggc ttgcccagac cggggtcgaa
     1621 ggcgaggagc ctgtagcgga agcggtcgcc atggtgggcg gccgtggcct cgacgctcac
     1681 gagggcgacc cggtagccgc cgagctcgaa atgctcgggc ttgccgaggg cgaggtggcg
     1741 catccagctc atgtcgcgga tggcttccat ggcttcgcag ctccttggct aaactatagc
     1801 agcgctcagt cccccaggca acgatgccgc gggccctggc cccggccttg ggagggatca
     1861 ggcggctccc cgtccttggc tggggcaggt gcggcggttt ctattctttt cctccccttg
     1921 cttctgggcg tcaatgtggg tataaggata gacgatgcgg cgagccctcg aggctcccgc
     1981 gatcccctcc ccgtatgggg cggtcaccca cgcccgggct tggcttcacg caggcctcgg
     2041 gcacgcgcga aaggggcacg atatgccgga ttccgaccac gcgtcgccgg aaccgcagaa
     2101 gccgtcacca tgcaagcccc gcgcggacgg ggtcgcccgg taattcagcg tggcgtcctc
     2161 cgtccccgat cggggcggga ggatcacatc catgctctcc acaacgacct tctatctgag
     2221 ccggatgctc ggggcgacag cgataggtcc cgacgaccgc cgggtcggcc gggtccgcga
     2281 catcgtcgtc gacctgagcc aggcccgtcc ccgtgtcctc ggaatcctcg cgggttcggg
     2341 ccggagcgag aggcttctgc ccttcgagga atgcctgccc accggaggga gcaggctccg
     2401 cgccgtgcgc acggggcccg cgggcgaggt gaggctcccc tccgagaacc tcatctatct
     2461 ccgcaaacac ctcctcgaca aacagatcgt cgacgttgac ggccgcaagc tcgtccgcgt
     2521 gaacgacctg gtgatcgaca cgacatcggc cggggcctac ctcgtcgccg tcgacgtggg
     2581 ggccgagggc ctcctccggc gcctcgggat cgaggggccc atcgccgccc tgctccgcgt
     2641 cttcgggacg acgatcccca gcaagctcgt gctctggggc gaggttgagg ccgtcgacct
     2701 gccgagcacc cagatcaagc tctccaaggc ctactcgaag ctggacggca tccacccctc
     2761 ggacctcgcc gacatcctcg aggatctgga ccgcggctcc caggtcgcca tcttcaccgc
     2821 gatggacgag gagagggcgg ccgacgtcct cgaggagctc gagcccgagg cccaggtaaa
     2881 gctcctcgag aacctctccg aggagaaggc cgccgacgtc ctcgagaaga tgccggcgga
     2941 cgaggtcgcg gacatcctcg atgagctccg gacggacaag gcggagcggc tcctcgcgga
     3001 gatggaggcc gagtcctcga ccgaggtccg gaacctcatg gcctatcccg agggcacggt
     3061 cggctccctc atgtccaagg actacgtctt cttcaaccaa tcgatgaagg tagctgacgc
     3121 cctggccgag ctgcgggcca tcaagcccga ggccgaggtc gtgtacagcc tcttcgtcct
     3181 ggacgcccgg ggcaagtacg tcgccaccct ctccctacgc gatctcgtcg tcgcagagcc
     3241 ggaggctaga ctgggccaaa tcatgagctc ggtggccgat cccctccacg acgacgatgc
     3301 cgcggacgtg ctgccggagc tcatgatcaa gtacaagctc cactccgcgc ccgtcgtgga
     3361 cgcggaaggc cgcatggcgg ggaccatcgt catcgaggac gtgctcgagc gcgttatgcg
     3421 caagcgctag agtctgcgcg cgaaatctac tcggagtacc attatgctca
//
LOCUS       DASSQV010000057         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1261266, whole genome shotgun sequence.
ACCESSION   DASSQV010000057 DASSQV010000000
VERSION     DASSQV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261266"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..671
                     /locus_tag="VFL04_01025"
     CDS             108..671
                     /locus_tag="VFL04_01025"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET7838310.1"
                     /translation="MKATIGAGDRGRTGLLSGERVAKDDPRIEALGELDELNSFIGLL
                     LASLSAAQSVFGQELEGIQADLFGMGAVLASSPASEGSELLDFGLPARTRALEAAMTG
                     LEAGLVPLREFLLPGGHPAAAMAQVARSVCRRAERRVVALVGTGPEAETHYAEALAYL
                     NRLSDYLFTLARACNMREGLPDRPWKR"
     gene            659..1327
                     /locus_tag="VFL04_01030"
     CDS             659..1327
                     /locus_tag="VFL04_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7838311.1"
                     /translation="MEALKPETSFRTRLLARTWLSSRVFECILERPEGFSFRPGQAIR
                     LSLAGVERDYSIASGPGEPGLRLCVKRIEGGALSPALAALPIGGEMGFSGPRGFFFIR
                     GSSRPRVLVATGTGVAPFRSMAETGLVGYTLLHGVREAGELFYRDRLEPGAARYVPCL
                     SAGPGQAVAGGFAGRVGAWVEAHLERRAWDFYLCGHRGMIRDLSLLVDESFPGSRLFC
                     EIFD"
     gene            1401..1805
                     /locus_tag="VFL04_01035"
     CDS             1401..1805
                     /locus_tag="VFL04_01035"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7838312.1"
                     /translation="MDRPTERDNNCFCCGADNEKGLHLVFSYPAQGEAEASLIVPEHF
                     SGWKRMTHGGFLSMLLDEVMAHACLGAAETAVTGEITVRFLKPVQTGTEVRLQGKVVE
                     KKGRIIHTEGRILDAAGESAAEGRARFVAART"
     gene            complement(1802..>2327)
                     /locus_tag="VFL04_01040"
     CDS             complement(1802..>2327)
                     /locus_tag="VFL04_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7838313.1"
                     /translation="AAHAGTAGAGFSVVADEIRSLAEKSQTQSKEVGRRLAEVKASID
                     SAVSSAQGASRGFDEVEGLIVTVSQFQEGIKTALQEQSTGSMEILEVLGSMNGVTQSV
                     RGGAGEMTQGAREVTAGMERLAELAVRTREEMRRITSDMGRMAESFRGMAAMIEENSG
                     AAKEVGAQIGRFRL"
BASE COUNT          327 a          831 c          790 g          379 t
ORIGIN      
        1 ccgcggtttc aaagaaccgg ggccccgggc caaggcggcc cttcctcggg aggcggaaaa
       61 gtccgatatt cgtccttggc gcgccaggcg acattggggg aggcggtatg aaagcgacga
      121 tcggggccgg cgaccgcggc cggacgggtt tgctgagcgg ggagcgagtg gccaaggacg
      181 acccgcggat cgaggccctt ggggagctcg acgagctcaa ctccttcatc gggctcctcc
      241 tcgcttcgct gtctgcggcc cagtccgttt tcgggcagga gctcgagggg atccaggccg
      301 acctctttgg gatgggcgca gtcctggcct cgagccccgc ttccgagggc tcagagcttc
      361 tggatttcgg cctccccgcc cgcaccaggg cccttgaggc ggccatgacg ggcctcgagg
      421 ccgggctcgt gcccctgcgc gagttcctcc tccccggagg ccaccccgcc gcggccatgg
      481 cccaggtggc gaggagcgtc tgcagacggg ccgagcgcag ggtcgtcgcc ctggtcggga
      541 cgggccccga ggccgagacc cactacgccg aggccctggc ctacctcaac cgcctctcgg
      601 actatctctt caccctcgcc agggcctgca acatgaggga ggggctgccc gacaggccat
      661 ggaagcgctg aagcccgaga cctcgttcag gaccaggctc ctcgcgagga cctggctctc
      721 ctcgcgggtc ttcgaatgca tcctcgagag gcctgagggt ttctccttcc gccctggcca
      781 ggcgatccgg ctctccctgg ccggggttga gcgtgactat tccatagcct cgggtcccgg
      841 cgagcccggg ctcagactct gcgtcaagcg catcgaggga ggggccttga gcccagcgct
      901 ggccgccctc cccatcgggg gggagatggg cttcagcggc ccgaggggtt tcttcttcat
      961 ccgcggatcg agccgtcccc gggtcctcgt cgcgacgggg acgggcgtgg cccccttccg
     1021 ctcgatggcc gaaaccggcc tcgtgggcta taccctcctc catggtgtgc gcgaggccgg
     1081 ggagctcttc taccgcgacc gtctcgagcc aggggccgct cgctatgttc cctgcctgtc
     1141 cgccggtccc ggccaggccg tggcgggagg cttcgccggc agggtcgggg cctgggtcga
     1201 ggcccatctc gagcgccggg cctgggactt ctacctctgc ggccaccgcg ggatgatccg
     1261 cgacctcagc ctcctggtcg acgagtcctt cccgggctcg aggctcttct gcgagatatt
     1321 cgactgaggg ccgggttgat ggcgggcggg cagagccgct accatggccc atgccgccga
     1381 tggatccgat ggggggaccg atggacagac cgaccgagcg cgacaacaac tgcttctgct
     1441 gcggagccga caacgagaag ggcctccacc tcgtcttcag ctatcccgcc cagggggagg
     1501 ccgaggcctc gctcatcgtg cccgagcact tctcggggtg gaagaggatg acccacggtg
     1561 gcttcctctc catgctcctc gacgaggtca tggcccacgc ctgtctcggg gcggccgaga
     1621 ccgccgtcac cggcgagatc accgtgaggt tcctgaagcc ggtccagacg gggactgagg
     1681 tccgcctcca gggcaaggtg gtcgagaaga agggccggat catccacacc gagggccgga
     1741 tcctcgacgc cgctggcgag agcgcggccg aggggagggc gcgcttcgtc gcggccagga
     1801 cctagagcct gaagcggcct atctgggccc ccacctcctt ggccgcgccc gagttctcct
     1861 caatcatcgc ggccatgccc ctgaaggact cggccatcct gcccatgtct gaggtgatgc
     1921 gccgcatctc ctcgcgggtg cgcaccgcga gctcggccag gcgctccatg cccgcggtga
     1981 cctccctcgc gccctgagtc atctccccgg ccccgcccct gaccgactga gtcacaccgt
     2041 tcattgaacc gaggacctcg aggatctcca tggaacccgt gctctgctcc tggagggcgg
     2101 tctttatccc ctcctggaac tggctcaccg tgacgatgag gccctccacc tcgtcgaagc
     2161 cgcgcgaggc cccctgggcc gaagagaccg cgctgtcgat cgaggccttg acctcggcca
     2221 ggcgccgccc tacctccttg gactgcgtct gcgacttctc ggcgagcgag cggatctcgt
     2281 ccgcgaccac ggagaagccg gccccggcgg ttccggcgtg ggccgcc
//
LOCUS       DASSQV010000058         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_888945, whole genome shotgun sequence.
ACCESSION   DASSQV010000058 DASSQV010000000
VERSION     DASSQV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888945"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VFL04_01045"
     CDS             <1..972
                     /locus_tag="VFL04_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838314.1"
                     /translation="VVAAPVDAVTLQFTYPGAAFTFGAAYTGLLFLDTSPFTISLADQ
                     ARSNADTELFGSRRLLGSARLDLPRIFGQSLALSAIVQDDLNNRSDFVATGSTSFDAN
                     KGGALNTQYFGLSVGGSISSLTYGTFFTYGTGRILSWLPDSLAPAGHSYQTAPISSFL
                     TGLSLQYPLASLNTSLGLRVLFASGDADATGPVEGNAASTYKRFTPLTRSSLGLVFSP
                     YLANLVFIEASASEKDLLLPGLSGSLKLMTFLRPTAGPISEPGLEPGSSAAYLGSEVD
                     ITAFYGIYTDLDVSCTIGAFLPGGAFDSGYAGTKFQMAATLAITLHM"
     gene            984..1703
                     /locus_tag="VFL04_01050"
     CDS             984..1703
                     /locus_tag="VFL04_01050"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET7838315.1"
                     /translation="MKKLAASILAILILVSSAGAATQAVLSGFKGKVEVKPDGGAWTA
                     ATKGMTVDLKTTISTGFDSTATILIDKSTIVVKPLTRLTLDKLVEQAGSVSASMFLRV
                     GAVQASVKAAVPGTPQDFKVQSPYSTASVRGTEFSFDGFYLRVVEGVVVLVPGRPTRE
                     AEGGATGGFEGATNVDPANASNGVPVAPGQEAALQMFFALDTGAHAVSGEGGTGETGK
                     APGAPPAAPARSGIVITVTGP"
     gene            1717..>2252
                     /locus_tag="VFL04_01055"
     CDS             1717..>2252
                     /locus_tag="VFL04_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838316.1"
                     /translation="MRKLTLLLAAAAVAIALGSCALETPAQGHVTVSVPRYLIAGSSA
                     ARVLEGGGNGGVTAIRLYIQLNGSFLKQAGGLDYFQTNLTAGSGSDANTITMDLPPTS
                     GYKILASLGTTTNGWTTLYVGETQTFTVAAGVFTNQNLPIQYFDPAPTPTLLTQIPTA
                     SRSSTVTVGSNSGAVSYWL"
BASE COUNT          368 a          855 c          644 g          385 t
ORIGIN      
        1 gtagtcgccg ctcccgtcga cgcggtaacc ctgcagttca cctatcctgg cgcggccttc
       61 accttcggcg ctgcctacac cgggctgctc ttcctcgaca cctcgccctt cacgatctcg
      121 ctcgccgacc aggcccgctc gaacgccgac accgagctct tcggctcgcg gcgcctcctc
      181 ggctcggccc gcctcgacct tccgcggatc ttcggccaga gcctggccct gtcggccatc
      241 gtccaggacg acctcaacaa ccgctcggac ttcgtggcga cgggcagcac ctccttcgat
      301 gccaacaagg gcggcgccct gaatacccag tacttcggcc tctctgtggg cgggtcgatc
      361 tcctcgctga cctatggcac cttcttcacc tacggcacgg gcaggatcct ctcatggctc
      421 cccgacagcc tggccccggc cggccattcc tatcagacgg cgcccatctc ctcattcctg
      481 acggggctct ccctccaata ccccctcgcc tcgctcaaca ccagcctcgg cctcagggtc
      541 ctcttcgcct cgggcgacgc cgacgccacg ggaccggtcg agggcaacgc ggcctcgacc
      601 tacaagcgct tcaccccgct cacccggagc agcctcggcc tcgtgttctc gccctacctc
      661 gccaacctcg tgttcatcga ggcctcggcc tcggagaagg acctcctcct ccccgggctc
      721 tcgggaagcc tcaagctcat gaccttcctg aggccgaccg ctggacccat ctcggagcca
      781 ggccttgagc ccggctcctc cgcggcctac ctgggaagtg aagtcgatat cactgctttc
      841 tatggaattt acaccgacct cgatgtatca tgtacgattg gggcctttct tccgggcggg
      901 gccttcgact ccggctatgc cggcaccaag ttccagatgg ccgccaccct ggcaatcacc
      961 ctgcacatgt gaggagcgaa gggatgaaga agctagcagc ttccattctc gcgattctga
     1021 ttttggtgtc gtcggcaggg gccgcgacgc aggccgtgct ctccggtttc aagggcaagg
     1081 tcgaggtcaa gcccgacggc ggggcctgga cggccgccac caagggcatg accgtggacc
     1141 tgaagaccac gatctccaca ggctttgact ccactgccac gatcctcatc gacaagtcga
     1201 ccatcgtggt gaaacccctg acccgactca ccctcgacaa gctcgtcgag caggcgggca
     1261 gcgtctcggc ctccatgttc ctgcgggtcg gcgctgtcca ggcgagcgtc aaggccgctg
     1321 tcccgggcac gccccaggac ttcaaggtcc agagccccta ctctacggct tcggtccgcg
     1381 gcaccgagtt ctccttcgac ggcttctacc tccgcgtggt agagggcgtc gtcgtcctcg
     1441 tgcccggacg gcccacccgc gaggccgagg gcggggccac gggaggcttc gagggcgcca
     1501 cgaacgtgga tccggccaac gcctccaacg gggtgccggt cgccccgggc caggaggccg
     1561 ccctccagat gttcttcgcc ctcgacaccg gggcccacgc ggtctcgggc gagggcggga
     1621 ctggtgaaac cggcaaggcc cccggagccc cgccggccgc cccagcgcga agcggcatcg
     1681 tcatcacggt caccggacca taaggaggca aaagccatga gaaagctcac actgctcctg
     1741 gcggccgccg cggtcgcgat agccctcgga tcctgcgcgc tcgagactcc ggcccagggc
     1801 cacgtcacgg tctcggtgcc ccgttacctg atcgcgggca gcagcgccgc gcgggtgctc
     1861 gagggcggcg gcaacggagg cgtcaccgcc attcgcctgt acatccagct gaacggctcc
     1921 ttcctcaaac aggccggggg cctcgactat ttccagacca acctcaccgc gggctcgggc
     1981 tcggacgcca acaccatcac catggacctg cccccgacct cgggctataa gatcctggca
     2041 tcccttggca ccacgaccaa cggctggacc acgctctacg tcggcgagac ccagaccttc
     2101 acggtggcgg caggggtgtt caccaaccag aacctgccga tccagtattt tgatccagcc
     2161 ccaaccccga ccttgctcac ccaaataccg acagcatccc gctcttcgac agtgaccgtc
     2221 ggcagcaatt ccggcgctgt gtcctattgg ct
//
LOCUS       DASSQV010000059         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2380889, whole genome shotgun sequence.
ACCESSION   DASSQV010000059 DASSQV010000000
VERSION     DASSQV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380889"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFL04_01060"
     CDS             complement(<1..441)
                     /locus_tag="VFL04_01060"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HET7838317.1"
                     /translation="MPQDRSLVERVAAGEVRAVARLISLLEDGDPEAAAALRALHRST
                     GRSHVVGITGPPGSGKSTLTDKLIRILRDSGSKVAVLAVDPSSPFSGGAILGDRLRMQ
                     DHGTDPGVFIRSLASRGRLGGLSRATAAAARVLDAAGYDWVIIET"
     gene            complement(455..853)
                     /locus_tag="VFL04_01065"
     CDS             complement(455..853)
                     /locus_tag="VFL04_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET7838318.1"
                     /translation="MEKKIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQTP
                     EQIVAATIQEDADVVAMSILSGAHNHLFPRVMELLTEKGVDDVLVVGGGVIPEDDYDF
                     LKSRGIAAIFGPGTPTASTIEFIRNNLKRK"
     gene            complement(863..2530)
                     /locus_tag="VFL04_01070"
     CDS             complement(863..2530)
                     /locus_tag="VFL04_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET7838319.1"
                     /translation="MSDMENLRKAVGAWNAGVDKAVAKYPERRKDFVTGSGAPVERLY
                     TPLELEGHDYAEDIGLPGEYPYTRGVQNTMYRGRFWTMRQYAGFASAEESNKRYKFLL
                     KAGQTGLSVAFDLPTQIGYDSDHALSAGEVGKVGVAIDSLADMETLFDGIPLDQVSTS
                     MTINAPAAVLLAMYIAVAEKQGVGPDKLNGTIQNDILKEYVARGTYIYPPAPSMRLIT
                     NIFEYCSKQVPNWNTISISGYHIREAGSTAAQEVAFTIADGIAYVDAAIKAGLGVDDF
                     GPRLSFFFNAHNDLFEEVAKFRAARRVWAKIMRDRFGAKNPKSLMLRFHTQTGGSTLT
                     AQQADNNIVRVAIQALAAVLGGTQSLHTNSKDEALALPTEDSVRIALRTQQIIANESG
                     AAETVDPLAGSYYVESLTNRIEAEALAYIKRIDELGGAVKAIEGGYVQKEIQESAYAY
                     QMAVEKGDRVVVGLNKFQVEEGKPTGLLRVDPSVGERQVEKIRALKAKRDGARVQKAL
                     GELEKAAKGDANLMPPILEAVRAYATLGEVCDVLRKVFGEYRPSVMF"
     gene            complement(2535..>2820)
                     /locus_tag="VFL04_01075"
     CDS             complement(2535..>2820)
                     /locus_tag="VFL04_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET7838320.1"
                     /translation="KKGLVIIDSSVEQDSSIKARTVALPILQTATEGVGKKVVANIVA
                     LGVLGGVSGIVQPGSLEAAVKNRVPKGTEDLNIKALRAGLELAARAKVQE"
BASE COUNT          377 a          917 c         1015 g          511 t
ORIGIN      
        1 cgtctcgatg atcacccagt cgtagcccgc agcgtcgagg acccgggccg ccgcggccgt
       61 ggcgcgggag aggcccccca gcctgcccct cgaggcgagg ctgcgtatga agacccccgg
      121 gtcggtcccg tggtcctgca tgcgcaggcg gtcgccgagg atggccccgc ccgagaaggg
      181 gctcgagggg tccacggcga ggacggccac cttcgagccc gagtcgcgga ggatgcggat
      241 gagcttgtcg gtgagggtgc tcttgcccga gccgggcggg cccgtgatgc cgacgacatg
      301 gctcctcccc gtcgaacggt ggagggcccg cagggccgcg gcggcctcgg gatcgccgtc
      361 ctccaggagg gagatgaggc gggccacggc gcggacctcg cccgcggcca cgcgctcaac
      421 taggcttcga tcttggggca tctggagggc gccgctactt gcgcttgagg ttgttcctga
      481 tgaactcgat cgtcgaggcc gtcggggtgc cggggccgaa gatggccgcg atgcccctgg
      541 acttcaggaa gtcgtagtcg tcctccggaa tgaccccgcc gcccacgacg aggacgtcgt
      601 cgacgccctt ctccgtgagg agttccatca cccgcgggaa gaggtggttg tgggcgccgg
      661 agaggatgct catggcgacc acgtcggcgt cctcctggat cgtcgccgcg acgatctgct
      721 cgggcgtctg cctgagcccg gtgtagatga cctccatgcc ggcgtcgcgc agggccctgg
      781 ctatgacctt ggcccccctg tcgtgtccgt cgagcccggg cttggcgacc agcactcgta
      841 tcttcttttc catcattctc tcctagaaca tcacgctcgg gcggtattcg ccgaagacct
      901 tgcggagcac gtcgcagacc tcgcccaggg tggcgtaggc ccgcacggcc tcgaggatcg
      961 gcggcatgag gttggcgtcg cccttggcgg ccttctcgag ctcgcccagg gccttctgga
     1021 ccctggcccc gtcgcgcttg gccttgaggg ccctgatctt ctcgacctgg cgctccccga
     1081 ccgaggggtc gacgcgcagc aggcccgtgg gcttgccctc ctcgacctgg aacttgttga
     1141 gccccacgac gacgcggtcg cccttctcga cggccatctg gtaggcgtag gccgactcct
     1201 ggatctcctt ctggacatag ccgccctcga tcgccttgac ggcgccgccg agctcgtcga
     1261 tgcgcttgat ataggcgagg gcctcggcct cgatgcggtt cgtgaggctc tcgacgtagt
     1321 aggagcccgc gagggggtcc accgtctcgg ccgcgccgga ctcgttggcg atgatctgct
     1381 gggtccgcag cgcgatgcgc accgagtcct cggtgggcag ggccagggcc tcgtctttcg
     1441 agttcgtgtg cagcgactgg gtgcccccga gcacggcggc cagggcctgg atcgcgacgc
     1501 ggacgatgtt gttgtcggcc tgctgggcgg tgagggtcga gccccccgtc tgggtgtgga
     1561 agcggagcat gaggctcttc gggttcttgg ccccgaagcg gtcgcgcatg atcttcgccc
     1621 agaccctgcg cgcggcgcgg aacttggcga cctcctcgaa gaggtcgttg tgcgcgttga
     1681 agaagaatga gagccggggc ccgaagtcgt ccaccccgag cccggccttg atggcggcgt
     1741 cgacgtaggc gatgccgtcg gcgatggtga aggccacctc ctgggccgcg gtggagccgg
     1801 cctcgcggat gtggtagccg gagatggaga tcgtgttcca gttgggcacc tgcttggagc
     1861 agtactcgaa gatgttggtg atgagcctca tcgagggcgc gggcggatag atgtaggtgc
     1921 cgcgggccac gtactccttg aggatgtcgt tctggatcgt cccgttgagc ttgtcggggc
     1981 ccacgccctg cttctcagcg accgcgatgt acatggcgag gaggacggcc gccggggcgt
     2041 tgatggtcat gctcgtggac acctggtcca gggggatgcc gtcgaagagg gtctccatgt
     2101 cggcgaggga gtcgatcgcg acgcccacct tgccgacctc gccggctgag agggcgtggt
     2161 cggagtcgta gccgatctgg gtgggcaggt cgaaggcgac cgagagcccg gtctggccgg
     2221 ccttgaggag gaacttgtag cgcttgttcg actcctcggc gctggcgaag ccggcgtact
     2281 gccgcatggt ccagaagcgg ccgcggtaca tcgtgttctg cacgccgcgg gtgtaggggt
     2341 actcgcccgg caggccgatg tcctcggcgt agtcgtggcc ctcgagctcg aggggggtgt
     2401 agagccgctc caccggggcg cccgagccgg tgacgaagtc cttgcggcgc tcggggtact
     2461 tggccacggc cttgtcgacc ccggcgttcc aggctcccac ggccttcctg aggttttcca
     2521 tgtcgctcat cgcatcactc ctggactttc gcccgggcgg cgagctcgag cccggccctg
     2581 agcgccttga tgttgagatc ctcggtcccc ttgggcaccc ggttcttcac cgccgcctcg
     2641 aggctgccgg gctggacgat gccggagacg ccgcctagga cgccgagggc cacgatgttg
     2701 gccaccacct tcttgccgac cccctcagtg gcggtctgga ggatggggag ggcgaccgtg
     2761 cgcgccttga tcgagctgtc ctgctccacc gaggagtcga tgatcacgag gcccttcttc
//
LOCUS       DASSQV010000060         5225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2256327, whole genome shotgun sequence.
ACCESSION   DASSQV010000060 DASSQV010000000
VERSION     DASSQV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256327"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..793
                     /locus_tag="VFL04_01080"
     CDS             116..793
                     /locus_tag="VFL04_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7838321.1"
                     /translation="MEASLNEAARLYALRDWEGTLESLRGVESTPSNYLDLAYFLGLC
                     HARLSHWDDALLYLEQVVTASEDLMRIFQCRLALAYVYSVTGRHKLAEYELGRLLESG
                     LESAQGLCSLGYTAWAQNRAEEAIRDYTQALELDPENATALNGLGYILACEGKDAARA
                     LTYCRKAVDKRPDNPAYLDSLAWAYFRLGFIPEAKDYLGRALAKAPGEIEIQRHARSI
                     EGVEDAR"
     gene            790..2868
                     /locus_tag="VFL04_01085"
     CDS             790..2868
                     /locus_tag="VFL04_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HET7838322.1"
                     /translation="MKCRSLVALILVVLVGPPALAQFNSELDSPGAAEQLRLGVQAFN
                     RGRFGESILLLEKALAYAPKQSLISYWLGRAYFKSGFEETALRAWQPLLEMPDAIPSL
                     RAKMEIIRASRSIPPATEAYRYVEVARFEGRRGKDDFFLRPSAILPRRDGSILVVAHG
                     SNELVTIDAGGIVRRRDRGGLAGFDGPFGAAALPDGTLFVTEFGGDRIARIAPGGKVT
                     TFGGKGRGPGTLLGPQFAAADAAGYLYVADYGNARVSKYDSEGNFVLSFGDKQPESGF
                     GGLASPAGILVQDAVVYIADSLRRAIYRFDLSGNYLGAMAEGELHFPEGLASWQSGRE
                     LLVSDTDRVVSIDLGTEATRLVYSSPDPKARIVGAAPDFNGNLAICDFDASVVSLLSE
                     SSLIASGYDVEIERIVSDAFPVVDIDVNVRDRNGAPVVGLREGNFYLTERIKRTTQSD
                     ESGKTVLRKEETLVPAADLSLVGAGQVPSGSRTTILVERSKALSPQLDGLRSALIELY
                     RVLGAEGAKPSLVTAGAVPALESAGDITAAVRAATAPAAGNGRIDLGLRLAATGLLPS
                     GSRDAVVYLGTGQVDEASFTGTTLSELAALLRNNGIRFYAVVLGQPSPGLRYLVERTG
                     GAIYAADRPRGLGDLGADLRSAPSGRYRLRFTSKADSEFGKAYLSVGIEAYLYKKSGK
                     DELGYYAPLR"
     gene            2930..3002
                     /locus_tag="VFL04_01090"
     tRNA            2930..3002
                     /locus_tag="VFL04_01090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2963..2965,aa:Thr,seq:tgt)
     gene            3031..3113
                     /locus_tag="VFL04_01095"
     tRNA            3031..3113
                     /locus_tag="VFL04_01095"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3065..3067,aa:Tyr,seq:gta)
     gene            3161..3649
                     /locus_tag="VFL04_01100"
     CDS             3161..3649
                     /locus_tag="VFL04_01100"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET7838323.1"
                     /translation="MGEPFYVDGLRFGCTRCSACCGGEPGYVFLSKDDLRRLLDRFKL
                     DFGEFFREYCRLVDTGLGMAISLAEKPNYDCTFLGEGGCSVYDARPVQCSTYPFWAGV
                     LESPQTWQSEASYCPGIGKGELVGRDLIERRLSERKTAGTILLAYGADPETLSADKVL
                     GS"
     gene            3708..4562
                     /locus_tag="VFL04_01105"
     CDS             3708..4562
                     /locus_tag="VFL04_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7838324.1"
                     /translation="MQRITPKDLVSQEARERFLASLPDYLFSTVGGNTACVEVATGEQ
                     RRLIFDAGTGIRELGAQIAATKRPESCHIFFSHFHWDHVQGLPFFSPAFDSRSTIDFY
                     SPVPGFRDILERQMRSPSFPITMEAMGARKHFHVLSGDPVRVGAVEVRYRQMSHPGGC
                     YSYEVRENGVKVIYSTDTELREEDFRRTPENAEYFQDADALIIDTQYTLGEAIEKYNW
                     GHSSFSLAADFASTWGIKRLILFHHEPSYSDRKLEGICKNAEWYIEHLGGPGTSVSLA
                     REGLEIEV"
     gene            4566..4763
                     /locus_tag="VFL04_01110"
     CDS             4566..4763
                     /locus_tag="VFL04_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838325.1"
                     /translation="MARFTVDLDVPDALRLYLFVCGREEELQGPTAAFAERLRAFLYE
                     HLSIEDMEHPKDCLARLTRDR"
     gene            4782..>5225
                     /locus_tag="VFL04_01115"
     CDS             4782..>5225
                     /locus_tag="VFL04_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET7838326.1"
                     /translation="MPATRKPRALTALLLPAGLLVLAIVLGLAGMAFLLSASASIPAE
                     GKLFTVERGESALSVAQRLEDEGLIRSALGFRLLGRIEAAGSGLKAGTYRIESGMGSP
                     AILDLLRSGRQALVRLTLPEGFTLGQTARLLDRLGIAKAPDFQAAC"
BASE COUNT          885 a         1748 c         1620 g          972 t
ORIGIN      
        1 aggggacaga ccatggggcg gtcgcagcgg ggagggtggc cgtgagcccc gtcctcgtcc
       61 atccccaggt tccggcgggc ttcgtgtccg gcgcggcgtt tggatagggt gaccaatgga
      121 agctagcctc aacgaggccg caaggctgta tgccctcagg gactgggagg ggacgctgga
      181 gtccctgagg ggcgtcgaat cgaccccgtc caactacctc gatctcgcct atttcctcgg
      241 cctctgccat gcgaggctgt ctcattggga cgatgccctt ctctaccttg agcaggtcgt
      301 cacggcgagc gaggacctca tgcggatctt ccagtgcagg ctcgccctcg cctatgtcta
      361 cagcgtcacc gggaggcaca aactcgcgga gtacgagctg ggccgcctcc tcgaaagcgg
      421 cctcgaatct gcccagggcc tctgttccct tggttacacc gcctgggccc agaatcgggc
      481 cgaggaggcc atccgcgatt acacccaggc cctcgagctt gaccccgaga acgcgacggc
      541 cctgaatggc ctcggctaca tcctcgcctg cgagggcaag gacgccgcca gggcactgac
      601 ctattgccgg aaggccgtcg acaagcgtcc cgacaatccc gcctacctgg attccctagc
      661 ctgggcctac ttccggctcg gattcatccc ggaggccaag gattacctcg gccgcgccct
      721 cgccaaggcc cccggcgaga tcgagataca aaggcacgcc cggtcaatag agggagtgga
      781 ggatgcccga tgaaatgccg tagcctcgtc gccctcatcc tcgtcgtact tgtgggccca
      841 cccgcccttg cgcagttcaa cagcgagctg gactcccccg gtgccgccga gcagctcagg
      901 ctgggagtcc aggccttcaa ccgtggcagg ttcggagaat ccatcctcct gctcgagaag
      961 gccctcgcct acgcgccaaa gcaatccctc atcagctact ggctcggtcg ggcctatttc
     1021 aagtcgggct tcgaggagac cgccctcagg gcctggcagc cactgctcga gatgcccgat
     1081 gccatcccct cccttcgcgc caagatggag ataatcagag cctcccgctc aatccccccg
     1141 gcgactgagg cgtaccgcta tgtcgaggtg gcgaggttcg agggcaggag gggaaaggac
     1201 gatttcttcc tgaggccatc ggccatcctc ccgaggaggg acggatcgat cctcgtcgtc
     1261 gcccacggct cgaacgagct cgtgacgatc gacgccggcg gcatcgtcag gagacgggac
     1321 cgcggcggct tggcgggctt tgatggtccc tttggagccg ctgccctgcc cgatggcacc
     1381 ctgtttgtga ccgagttcgg gggggatcgg atcgccagga tcgccccggg cggaaaggtc
     1441 acgaccttcg gcggcaaggg ccgcggcccg ggcaccctgc tcggccccca attcgccgcc
     1501 gccgatgccg cgggctatct ctacgtcgcc gattacggca acgcgcgggt cagcaaatat
     1561 gactcggaag ggaatttcgt cctttccttc ggggacaagc agcccgaatc gggcttcggc
     1621 gggctagcca gcccggcggg catactggtg caggacgccg tggtctacat cgcagactcg
     1681 ctccgaaggg ccatctaccg tttcgacctc agcggcaatt acctgggcgc catggccgag
     1741 ggcgagcttc acttccccga gggcctcgca tcctggcagt ccggtaggga gctactcgtc
     1801 tccgacactg accgcgtcgt ctcgatagac ctcggaaccg aagccacaag gcttgtgtac
     1861 agctcccccg atcccaaggc gcggatagtg ggtgccgcgc cggatttcaa cggcaacctc
     1921 gcgatatgcg atttcgacgc ctccgtggtc tccctgcttt cggagtcatc cctcatcgct
     1981 tcgggctatg acgtggagat cgagcggatc gtctcggacg ccttccccgt ggtcgacatc
     2041 gacgtcaacg tcagggacag gaacggtgcc ccggtcgtgg gtctgcgcga gggcaatttc
     2101 tacctcacgg agcgcatcaa gcggaccacc cagtccgacg agtcagggaa gacggtcctc
     2161 cgaaaggagg agaccctggt gcccgcggcc gacctctccc tggtcggagc tgggcaggtg
     2221 ccctccggaa gcaggaccac gatcctggtc gagcggtcaa aagccctgtc ccctcagctc
     2281 gatgggctcc gcagcgccct catcgagctt tatcgtgtgc tcggcgccga gggcgccaag
     2341 ccctcccttg tgacggcggg ggcagtcccg gccctcgagt cggccggcga catcacggct
     2401 gcagtccgtg ccgccacggc tccagccgcg ggaaacggac gcatcgacct tggccttcgt
     2461 ctggccgcca ccggcctcct cccctcgggc agcagggatg ccgtcgtcta tcttgggacg
     2521 gggcaggtcg atgaggcctc gttcaccggc acgacgctct cggagctcgc cgccctgctg
     2581 cggaacaacg ggatccggtt ctacgccgtc gtcctcgggc agccttcccc cgggctgcgc
     2641 tacctcgtcg agaggacggg cggggccatc tacgccgcgg acagaccgcg gggcctcgga
     2701 gatctcgggg ccgacctcag gtcggcccca agcggacgct acaggctccg cttcacctcg
     2761 aaggccgact cggaattcgg aaaggcgtac ctgtcggtcg ggatcgaggc ctatctctat
     2821 aagaagagcg gcaaggacga gctgggctac tatgcgccat tgcggtagaa gatggctgaa
     2881 gctcttgact catgagaatg gttgcgtgta tagtgccgcc gtcgtcgagg ccgtcttagc
     2941 tcagttggca gagcgcgtga cttgtaatct cgaggtcccc ggttcgattc cgggagacgg
     3001 ctcgggtctg ctaagcgaac tcgatcccct ggggagattc ccgagtggtc aaagggggca
     3061 gactgtaaat ctgttggctc acgccttcga aggttcaaat ccttctctcc ccaacgcccc
     3121 ttctcctcgg agaaggggct ttttgtagat gaggctgccc atgggcgaac cattctatgt
     3181 cgatggcctt cggttcggat gcacccgctg ttccgcatgc tgcggtggcg aaccgggcta
     3241 cgtattcctt tccaaagacg acctcaggcg actcctcgac aggttcaagc ttgatttcgg
     3301 ggagttcttt cgcgagtatt gtcgcttggt ggacacgggc ctcggcatgg ccatcagcct
     3361 ggccgagaag cccaactacg actgcacgtt cctcggcgaa ggcggctgtt ctgtgtatga
     3421 tgcgcggccc gtccagtgtt cgacctaccc cttctgggcc ggggtcctcg agtcccccca
     3481 gacctggcag tccgaagcgt cctattgccc cggcatcggg aagggggagc tcgtgggccg
     3541 ggacctcatc gagcggcgtc tttccgagcg gaaaacggcc ggcaccatcc tcctggcata
     3601 tggcgcggat ccggagaccc tgagtgcgga taaagttctg gggagttagg ggctcgaccc
     3661 ccacgcccct catgcccgcg cgtctccgca gcaagatttc ctcggtcgtg caacgcatca
     3721 cgcccaagga cctggtgagc caggaggcga gggagcgttt cctggcctcg ctgcccgact
     3781 atctgttctc gaccgtggga ggcaacaccg cgtgcgtgga ggtcgccaca ggggagcagc
     3841 gccgcctgat attcgatgcc gggaccggaa tacgcgagct aggggcacag atcgccgcga
     3901 ccaagaggcc cgagtcctgc cacatcttct tctcgcattt ccattgggac cacgtgcagg
     3961 gcctgccatt cttctctccc gccttcgatt cccgctccac catcgatttc tacagcccgg
     4021 tccccggctt cagggacatc cttgagcgcc agatgcgctc tccgtccttc cccatcacca
     4081 tggaggccat gggggcccgc aaacacttcc acgtcctctc tggcgacccg gtccgcgtcg
     4141 gcgcggtcga ggttcgctat cgtcagatga gccatccagg cggctgttac agctacgagg
     4201 tccgcgagaa tggtgtcaag gtcatctact cgaccgacac cgagctccgg gaggaggatt
     4261 tccgcaggac cccggagaac gccgagtatt tccaggacgc cgatgccctt ataatcgata
     4321 cccagtacac tcttggcgag gccatcgaga agtacaactg gggacactcc tccttcagcc
     4381 tcgccgccga ttttgcctcg acctggggga tcaagcgtct catcctcttc caccacgaac
     4441 cctcatactc ggatcggaaa ctcgagggca tctgcaagaa cgctgaatgg tacatcgagc
     4501 acttgggggg cccgggcaca agcgtgagct tggcgcgcga gggcctcgag atcgaggtat
     4561 agtccatggc gcgcttcacc gtcgatctcg atgtccccga tgccctgagg ctctacctct
     4621 tcgtctgtgg gcgtgaggag gagcttcagg gtcccaccgc cgccttcgcc gaaaggctcc
     4681 gcgcctttct ttacgagcat ctctcgatcg aggacatgga acaccccaag gactgcctcg
     4741 cgcgcctcac acgagatcgt tgatcggagg atcgcttttc gatgccagct accagaaaac
     4801 cccgggccct cacggccctg ctccttcctg ccggcctcct ggttctcgcc atcgtgcttg
     4861 gccttgcagg catggccttc ctcctttcgg cctcggcctc catcccagcc gagggcaagc
     4921 tcttcacggt cgagagaggg gagagtgccc tgtccgtggc gcagcggctc gaggacgagg
     4981 gtttgatccg ctcagccctc ggtttcaggc tcctcggccg aatcgaggct gccggctcgg
     5041 ggctgaaggc cggaacctac cggatcgaat ccgggatggg aagcccggcc atcctcgacc
     5101 tgcttaggtc gggtcgacag gccctcgtga ggctgaccct gcccgagggc tttaccctcg
     5161 gccagactgc ccggcttctc gaccggcttg ggatcgccaa ggccccggat tttcaggccg
     5221 cctgc
//
LOCUS       DASSQV010000061         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_889389, whole genome shotgun sequence.
ACCESSION   DASSQV010000061 DASSQV010000000
VERSION     DASSQV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889389"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFL04_01120"
     CDS             <1..571
                     /locus_tag="VFL04_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838327.1"
                     /translation="VIVPDSSGDNSFANADERTILGCLAVRSDNPELSVSAEILRPES
                     EQHLKRAKVENIVINGEFSGYVLSAASVSKGLPRAARALLSPGTGPRLREASLPPALV
                     GKTFAEASSWFVANGKGVLVGLLSERKGVSLDDLLSDNSSAIDTFIKRKFMEAEVDLA
                     AAASAGSAVHLAPGPDHLIAEGDLAFVIG"
     gene            581..1096
                     /locus_tag="VFL04_01125"
     CDS             581..1096
                     /locus_tag="VFL04_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7838328.1"
                     /translation="MAISEKEAGLLRGVGILRDLSGEEIAKVISIARRVLVKAGGAIM
                     HEGEQGETMYLFIKGEVDVTKNLTLKLGERDFAKTEKSMNKLSSDRASVFGEMAMFGP
                     EPRSATITASSDCVLYEINRADFLALCDENPRIGLSIIRRIAGVLCSRVRKGNEDVLK
                     LSTALSIALSR"
     gene            1105..1692
                     /locus_tag="VFL04_01130"
     CDS             1105..1692
                     /locus_tag="VFL04_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838329.1"
                     /translation="MSIDPDTGLRPIPAWLLAAALAAIILLSTALPGDAQDKKPATPL
                     PRAVHGIELGMDRDAVIAALKKESLFLYRGPEDVSLLPSPNQSLIDVAGPSFVKHAYF
                     QFLDNKLWVMILVLNPDRIDHYSIYTSLVAKYGEPNSLDPKESRWEDGAVRMALERPL
                     TLRYMDLAVYSKLKEGSAAQGSAVEIDRQDFLGQL"
     gene            complement(1743..>2330)
                     /locus_tag="VFL04_01135"
     CDS             complement(1743..>2330)
                     /locus_tag="VFL04_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7838330.1"
                     /translation="RNRRIAARGLAPYSAHRAFRHFWIAYQDVMIAAQSACTAADSLG
                     LGSCYIGTIVEHLEASSELLGLDERTVPIELLPLGYPVSPPKSRGKFPEGILVHRERY
                     AEPEEAGLYSSYQEREGGWTMSLTPENLERFRDCCESVEGSEFAQRAVADAEARGAFN
                     AVQYRFGLHYPADEMPMGNAGFLEALKNRGFGAFG"
BASE COUNT          386 a          847 c          736 g          361 t
ORIGIN      
        1 cgtcatcgtc cctgatagct cgggcgacaa cagcttcgcc aacgccgacg agcgcacgat
       61 cctcggctgc ctcgccgtcc gctcggacaa ccccgagctc tccgtcagcg ccgagatcct
      121 caggcccgag agcgagcagc acctgaagcg ggccaaggtc gagaacatcg tcatcaacgg
      181 cgagttctcg ggctatgtcc tctcggcggc ctcggtctcg aagggccttc cgcgcgccgc
      241 gagggccctc ctctcccccg gcaccgggcc ccgcctcagg gaggcctccc tgcccccggc
      301 cctggtcggc aagaccttcg ccgaggcctc gagctggttc gtggcgaacg ggaagggcgt
      361 ccttgtgggt ctgctctccg agcgcaaggg ggtctccctc gacgacctgc tttcggacaa
      421 ctccagcgcg atagacacct tcataaagcg gaagttcatg gaggccgagg tcgacctcgc
      481 cgctgcggcc tcggcgggca gcgccgtcca cctcgcgccc ggccccgacc acctcatcgc
      541 cgagggcgac ctggccttcg tgatcggata gggggctgac atggcgatca gcgagaagga
      601 ggccggcctc ctccgcggcg tggggatcct cagggacctc tcaggcgagg agatcgccaa
      661 ggtgatctcg atcgcgaggc gggtcctcgt gaaggccggc ggggccatca tgcacgaggg
      721 cgagcagggc gagacgatgt acctcttcat aaagggcgag gtcgacgtga ccaagaacct
      781 caccctcaag ctcggcgagc gcgatttcgc caagaccgag aagtccatga acaagctgag
      841 ctccgaccgg gcctcggtct tcggcgagat ggcgatgttc ggccccgagc cgcggagcgc
      901 caccatcacg gccagctcgg actgcgtcct ctacgagatc aacagggccg acttcctggc
      961 cctctgcgac gagaacccca ggatcggcct ctccataatc agaaggatcg cgggcgtgct
     1021 ctgctcccgc gtccgcaagg gcaacgagga cgtgctcaag ctgtccacgg ccctcagcat
     1081 agccctgtcc cgatgaggaa tcccatgagc atcgaccccg acaccggcct ccgccccatc
     1141 cccgcctggc tcctggccgc ggcgctcgcc gccatcatcc tcctctcgac cgccctcccc
     1201 ggcgacgccc aggacaaaaa gcccgccacg ccccttccgc gcgccgtcca cgggatcgag
     1261 ctcggcatgg accgcgacgc cgtcatcgcg gccctcaaga aggaatccct cttcctctat
     1321 cgcgggcccg aggacgtgtc cctcctgccc tcgcccaacc agagcctcat cgacgtggcc
     1381 ggcccctcct tcgtcaagca cgcctacttc cagttcctcg acaacaagct ctgggtcatg
     1441 atcctcgtcc tcaaccccga caggatcgac cactactcaa tctacacgag ccttgtggcc
     1501 aagtacggtg agcccaactc cctcgacccc aaggaatccc gctgggagga cggtgcggtc
     1561 cgcatggccc tcgagcgccc cctcaccctc cgctacatgg acctcgccgt ctattcgaag
     1621 ctcaaggagg gctcggccgc ccagggctcg gccgtggaga tcgaccgcca ggacttcctc
     1681 ggccagctct aggagcctgg gggatcctcc cggcgcggcg tccccccaag gccggcgccg
     1741 gactagccga aggctccgaa gccccgattc ttgagcgcct cgaggaatcc cgcgttgccc
     1801 atgggcatct cgtcggcggg atagtggagg ccgaagcggt attggacggc gttgaaggcc
     1861 ccgcgggcct cggcgtcggc caccgcacgt tgggcgaact cggagccctc gacgctctcg
     1921 cagcagtcgc ggaaacgctc gaggttctcg ggggtcaggg acatggtcca gccgccctcc
     1981 ctctcctggt aggacgagta aagcccagcc tcttcgggct cggcatagcg ctccctgtgg
     2041 accaggatcc cctcggggaa cttgccgcgg ctcttcgggg gtgagacggg gtagccgagg
     2101 ggcaggagct cgatcggcac ggtgcgctcg tccaggccga ggagctcgga gctcgcctcg
     2161 aggtgctcga cgatcgtccc gatgtagcat gagccgaggc cgagcgagtc ggccgccgtg
     2221 cacgcgctct gggcggctat catgacgtcc tggtaggcga tccagaagtg cctgaaggcc
     2281 ctgtgggccg agtagggggc gaggcccctc gcggcgatgc gccggttcct
//
LOCUS       DASSQV010000062         3258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2256564, whole genome shotgun sequence.
ACCESSION   DASSQV010000062 DASSQV010000000
VERSION     DASSQV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256564"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VFL04_01140"
     CDS             complement(<1..1292)
                     /locus_tag="VFL04_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7838331.1"
                     /translation="MQKRVLIHHSDNPFLPANMLMDQVFLTGLRASPSLNVTIYSEYL
                     ELVRFSEADMLKESIELIKARYSAMDLDLVILTDDFSWDFYAKNASVFHAKAAVICGI
                     TEGYLDPKTLPRGVTGNFKRLDIGASLEDILSLHPGVREVVAITGTSPQDKVYSDIAR
                     RAFAAYAGRLRTRVLRDYSLEQMGDIVSELPPDSVILYLTVYRDGAGKSYNPRDALVY
                     LSKRAKVPIHGVSDTYLGYGIVGGNLVSFRDFTKDAAATAMAILGGRDPSELPAHPFV
                     NANYFDANELARWGIPDRLLPPNSVIINRTISPWQQYRWQILATLLFIVMETMLVVGL
                     VYSRRQLKLSLSQLGLSEERFRRAISEAPFPIMIQADDGRIEAISRTWTEITGYSHED
                     IPCLSEWTRLAYGERGGAVKANIDALFTADSRVDEGEFT"
     gene            complement(1414..1917)
                     /locus_tag="VFL04_01145"
     CDS             complement(1414..1917)
                     /locus_tag="VFL04_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838332.1"
                     /translation="MQVREMSRNPLVYALALLIATAFSGQGSGASDDQSARPAEAGGA
                     SPIVPVQGEAKPALPFIGNWRVVMVGLTDPKTGASKLSSPESRHYTFYIDRTGEITKG
                     TISAMFSWTWDGKNLSLRFRDGGAETYSSVISASSNGGTSLGLTGEFDYNRDGHPMAV
                     TMSLWKE"
     gene            2011..2676
                     /locus_tag="VFL04_01150"
     CDS             2011..2676
                     /locus_tag="VFL04_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838333.1"
                     /translation="MMTKELSLVHLIREPITAARGAPPLLLLLHGVGSNELDLMELVP
                     KLDDGFFCVSARAPFRQGEDAYAWFHVEMTPSGPVIRPREAESSREKLIGFIDELVEA
                     YGLNPRKVFLMGFSQGAVMSLSVALTRPDKVAGIVAMSGRLLPEVLPLVAEVKALTGL
                     PIFVAHGTMDAVLPIAHGRLARDKLGAMRVDLDYREYSMGHEVSAQSLGDISVWLSER
                     LRS"
     gene            complement(2684..>3258)
                     /locus_tag="VFL04_01155"
     CDS             complement(2684..>3258)
                     /locus_tag="VFL04_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838334.1"
                     /translation="VLVGSQVSPAEGGIEGERAALLRLVGELYAHDHMGEGEMTRRME
                     AIAHAASLEELRSQAGSMPAAGTGEAFLQTSLIERPVQRVAGKGSVIRKKGAWIESRQ
                     VLLEGSGSNVRLDLRHYGEGQGLSLELELELRQSSVLVLVPKSFRVVLAVEDSSASVV
                     KTRGRESPDAPNTITARGRVQGCFVKFAYR"
BASE COUNT          497 a         1057 c         1112 g          592 t
ORIGIN      
        1 gtgaactctc cctcgtcgac ccgcgaatcg gccgtgaaca gggcgtcgat gttggccttg
       61 acggctccgc ccctctcgcc gtaggcgagc cgggtccact cggagaggca gggtatgtcc
      121 tcgtgcgagt atccggtgat ctcggtccag gtcctgctga tcgcctcgat cctgccgtcg
      181 tcggcctgga tcatgatggg gaagggggcc tcggagatgg cccggcggaa gcgctcctcg
      241 ctgaggccca gctggctgag gctcagcttg agctggcgcc gtgagtacac gaggcccacc
      301 accagcatcg tctccatgac gatgaagagg agggtggcga ggatctgcca cctgtactgc
      361 tgccaggggc tgatggtccg gttgatgatg acgctgttcg ggggcaggag cctgtccggt
      421 atcccccagc gcgcgagctc gttcgcgtcg aagtagttgg cgttcacgaa ggggtgggcc
      481 ggcagttcgg agggatccct gccccccagg atggccatgg cggtcgcggc ggcgtccttc
      541 gtgaagtccc ggaagctcac gaggtttccg cccacgatgc cgtagccgag gtaggtgtcg
      601 gataccccgt ggatcgggac cttggcccgt ttcgagaggt agaccagggc gtcccgcgga
      661 ttgtagctct tccccgcccc gtccctgtag acggtcaggt agaggatcac cgagtcgggg
      721 ggcagctcgg agactatgtc gcccatctgc tcgaggctgt agtccctgag gaccctggtc
      781 ctgagcctgc ccgcgtaggc tgcgaaggcc cgtctggcta tgtccgaata gaccttgtcc
      841 tgggggctcg tgccggtgat ggccacgacc tccctcaccc cgggatggag actgaggatg
      901 tcctccaggc tcgccccgat gtcgaggcgc ttgaagttgc cggtgacccc cctgggcagg
      961 gtcttcggat cgaggtagcc ctcggtgatg ccgcagatca ccgcggcctt cgcatggaac
     1021 accgaggcgt tcttggcgta gaagtcccag gagaagtcgt cggtgaggat cacgaggtcg
     1081 aggtccatcg ccgagtacct cgccttgatc agctcgatgc tctccttgag catgtcggcc
     1141 tcgctgaagc gcacgagctc gaggtattcg ctgtagatcg tgacgttgag cgagggcgat
     1201 gccctgagcc ccgtcaggaa gacctggtcc atcagcatgt tggccggcag gaaggggttg
     1261 tccgaatggt gtatgaggac cctcttctgc acaatggtcg ccttcgccgg cgggctgggg
     1321 gagggggcct gggccgggag ggatgcaagg gccaggcaca aggctgtggc cgtaaaccag
     1381 gatctgcgca tgcacggctc cgctgtcgtg ggactattct ttccagaggc tcatcgtcac
     1441 ggccataggg tggccgtcgc ggttgtagtc gaactcgccg gtcaggccca ggctcgtgcc
     1501 gccgttcgag ctcgccgaga tcacgctcga gtaggtctcc gctccgccgt cgcggaacct
     1561 gaggctgagg ttcttcccgt cccaggtcca ggagaacatg gcgctgatcg tgcccttggt
     1621 gatctctccc gtcctgtcta tgtagaaggt gtagtgccgc gattcgggcg agctgagctt
     1681 cgacgcgccg gtcttcggat cggtcaggcc gaccatgacg acgcgccagt tgccgatgaa
     1741 gggcagagcg ggtttggcct cgccctggac ggggacgatc gggctcgctc cgcccgcctc
     1801 ggccggccgg gcgctctggt cgtccgaagc ccccgaacct tggcccgaga aggccgtggc
     1861 gatgaggagg gcgagggcgt agaccagcgg attcctgctc atttccctga cctgcattgt
     1921 gagggcttct tcaagcagag tcaatgagcg ttcggcgggc tttcgcgcta tcattggctc
     1981 atggccatcg gggcccatct aggagggagc atgatgacga aagagctgtc gctcgtccac
     2041 ctgatccgcg agccgatcac cgccgccagg ggagccccgc ccctcctcct cctgctccat
     2101 ggcgtcggga gcaacgagct cgacctgatg gagctcgtcc ccaagctgga cgacgggttc
     2161 ttctgcgtca gcgccagggc ccccttcaga cagggcgagg acgcctacgc ctggttccat
     2221 gtcgagatga ccccaagcgg gccggtcata cggccccgtg aggccgagag cagccgcgag
     2281 aagctcatcg gcttcatcga cgagctcgtg gaggcctatg gcctcaaccc caggaaggtc
     2341 ttcctcatgg gcttcagcca gggcgcggtc atgagcctca gcgtcgccct tacccggcct
     2401 gacaaggtgg cgggcatcgt cgccatgagc ggccgcctcc ttcccgaggt cctgcccctc
     2461 gtcgccgagg tgaaggccct gaccggcctg cccatcttcg tggcccacgg gaccatggac
     2521 gccgtcctcc cgatagccca cgggaggctg gcccgggaca agctcggcgc gatgcgggtc
     2581 gacctggact atcgggagta ctccatgggc cacgaggtga gcgcccagag cctgggcgac
     2641 atctcggtct ggctcagcga gaggctgcgg tcctaggttc gccctacctg taggcgaact
     2701 tgacgaaaca gccctggacc ctgccccggg cggtgatggt gttgggcgcg tcggggctct
     2761 ccctgcccct cgtcttgacc acgctggccg aggagtcctc cacagccagc acgaccctga
     2821 agctcttggg cacgaggacg aggaccgagg attgcctgag ctcgagctcg agctcgagcg
     2881 acaggccctg gccctcgccg taatgcctga ggtcgaggcg gacgttcgag cccgagccct
     2941 cgaggaggac ctggcgcgat tcgatccagg cccccttctt gcggatcacg ctccccttgc
     3001 cggccacccg ctggacgggg cgctcgatga gggaggtctg gaggaaggcc tcgcccgtcc
     3061 ccgcggccgg catcgagccg gcctgggacc tgagctcctc gaggctggct gcgtgggcga
     3121 tggcctccat ccgcctcgtc atctccccct cgcccatgtg gtcgtgggcg tagagctccc
     3181 cgacgaggcg gaggagggcg gccctctccc cctcgatccc cccttcggcc gggctcactt
     3241 gggagccgac caggacct
//
LOCUS       DASSQV010000063         3310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_267142, whole genome shotgun sequence.
ACCESSION   DASSQV010000063 DASSQV010000000
VERSION     DASSQV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267142"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1439)
                     /locus_tag="VFL04_01160"
     CDS             complement(6..1439)
                     /locus_tag="VFL04_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET7838335.1"
                     /translation="MDKARRQTILLVEDETLIAMMEKTQLEGLGYAVIHVTRGEEAVE
                     LACRGDEVDLVLMDIDLGAGIDGTVAAQEILECRELPILFLSSHLEKEVVDKTDKTSF
                     YGYVVKNSNFNVLRASIRMALRLSEADATIRKELAERRAVEAELLKSKERYQKDERIG
                     HLGNWELDIATGRVWVSDEARRIFGFDAESKDFSREDLASCITDRERVARALEGMAAG
                     DQPYSLEFEVRPRDGSEPRIIWSDAELVRDAQGRPAQVSGVIQDITTRKRSEQRIQAL
                     LEEKELLLKDVHHRVKNNMNTIYSLLSVQADRQADPGARQVLLDAAERVESMEILYDK
                     LYSSPNPASLSLRDYLPALVQEIVGVFPERARLRVVTSVEDIEVGAKLISNLGFIVNE
                     LVTNSMKYAFAEGEDGLVSLRVWRLAGDRVALRIEDSGPGLPETVSLEDSPGFGLQLV
                     GLLVKQIGGTVAVGRGKGTSFLIEFGA"
     gene            complement(1487..1834)
                     /locus_tag="VFL04_01165"
     CDS             complement(1487..1834)
                     /locus_tag="VFL04_01165"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7838336.1"
                     /translation="MSPLSAGGGGPALARAAPVFWALGDPTRLGLMARLSAEGPLSIA
                     RLTEGFGVTRQAITKQLAVLAGAGLVSDERRGRERVWSLEPGSVAEARRCLDEIAGQW
                     EGALERMRKYLED"
     gene            complement(1821..2270)
                     /locus_tag="VFL04_01170"
     CDS             complement(1821..2270)
                     /locus_tag="VFL04_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7838337.1"
                     /translation="MNSDRIEKSIVLKARRSRVWKAIADAEEFGSWFGIKFEGGFAAG
                     RRLKGQITTPGYTHLVFEIDVERIEPETLFSYRWHPYAVEPGVDYSVEPTTLVEFRLA
                     EVAGGTRVDITESGFDSLPAARRDLAFRMDDEGWAEQVESIEKYVAA"
     gene            2462..>3310
                     /locus_tag="VFL04_01175"
     CDS             2462..>3310
                     /locus_tag="VFL04_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838338.1"
                     /translation="MLEQRLVDWLLNSDPALRWQVERDLLGLPEAVWGATRARIATEG
                     LGSLLLELQDPDGQWAGGAFFPKDFEFQGPEAAEGAGQPWTATTWSLNTLRDWGLDPS
                     KLAGTAERLAANCRWEYEGLPYWGGEVDCCINSWTLSNGLWLGAEVDGLVDWFLAHQL
                     ADGGWNCEWVNGATCSSFHSTLNALKGLLDYERATLDRKRTARARRSGEEYLLQRGLY
                     RRLSTGMPVGPWALIFAYPFRWFYSVLNAADYFRAASIHDGRPPDGRMAEAIAIIEGA
                     RGPDGRW"
BASE COUNT          496 a         1165 c         1120 g          529 t
ORIGIN      
        1 gggactcagg ccccgaactc gatgaggaag ctcgtgccct tgccccgccc gaccgcgacc
       61 gtccccccga tctgcttcac gaggaggccc acgagctgga gcccgaagcc cggcgagtcc
      121 tcgaggctga ccgtctcggg caggccgggg cccgagtcct cgatcctgag ggcgaccctg
      181 tccccggcga gcctccagac cctgagcgag acgaggccgt cctcgccctc ggcgaaggcg
      241 tacttcatgg agttggtgac gagctcgttg acgatgaagc ccaggttcga gatgagcttg
      301 gcgcccacct cgatgtcctc gaccgaggtc acgacccgga gcctggcccg ctccgggaag
      361 acgcccacga tctcctggac gagggccgga aggtagtccc tcagcgagag cgaggcgggg
      421 tttggagagc tgtagagctt gtcgtagagg atctccatgc tctccacccg ttcggcggcg
      481 tcgaggagga cctgccgcgc gccagggtcg gcctggcggt ccgcctggac gctcaggagg
      541 ctgtagatcg tgttcatgtt gttcttgacg cggtggtgga cgtccttgag caggagctcc
      601 ttctcctcga ggagggcctg gatcctctgc tcggagcgtt tgcgcgtggt gatgtcctgg
      661 atgacgcccg agacctgggc cggcctgccc tgggcgtccc gaacgagctc ggcgtccgac
      721 cagatgatcc tcggctcgga gccgtccctg ggcctgacct cgaactcgag gctgtagggc
      781 tggtcccccg cggccatccc ctcgagggcc ctggccaccc gctcccggtc ggtgatgcag
      841 gaggcgaggt cctcgcgcga gaagtccttg gactcggcgt cgaagccgaa gatgcgccgg
      901 gcctcgtccg agacccagac ccgcccggtc gcgatgtcga gctcccagtt cccgagatgg
      961 ccgatcctct cgtccttctg gtagcgctcc ttggacttga ggagctcggc ctcgacggcc
     1021 cggcgctcgg ccagctcctt gcggatcgtg gcgtcggcct cggagaggcg gagggccatg
     1081 cggatcgagg ccctgagcac gttgaagttc gagttcttga ccacatagcc gtagaaggag
     1141 gtcttgtcgg tcttgtcgac cacctccttc tcgaggtggg aggagaggaa gaggatgggc
     1201 agctcgcggc actcgaggat ctcctgcgcc gccaccgtgc cgtcgatgcc cgccccgagg
     1261 tcgatgtcca tgaggacgag gtcgacctcg tcgccccggc aggcaagctc caccgcctcc
     1321 tcgccgcggg tgacgtggat caccgcgtag cccagaccct cgagctgggt cttctccatc
     1381 atggcgatga gggtttcgtc ctcgacgagg aggatggtct gccgccgggc tttgtccatg
     1441 ggactttggg aaaactatcg cgcacccggc cgcccctgta aatacgctag tcctcgaggt
     1501 acttccgcat gcgctccagg gcaccctccc actggccggc tatctcgtcg agacagcgcc
     1561 tcgcctcggc cacgctcccc ggctcgaggc tccagacccg ctcgcgcccg cgccgctcgt
     1621 cgctcacgag ccccgcgccg gcgaggaccg cgagctgctt cgtgatggcc tggcgcgtga
     1681 ccccgaagcc ctcggtgagc cgcgcgatgg acagcgggcc ctcggccgag agcctggcca
     1741 tgagcccgag ccgggtcggg tcgccgaggg cccagaagac gggggccgcc ctggccaggg
     1801 ccgggccgcc cccgcccgcg ctaagcggcg acatacttct cgatgctctc cacctgctcg
     1861 gcccagccct cgtcgtccat gcggaaggcg aggtcccgcc tcgccgccgg cagactgtcg
     1921 aagcccgact cggtgatgtc caccctcgtg cccccggcca cctcggccag gcggaactcc
     1981 acgagggtcg tgggctccac cgagtagtcg accccgggct cgacggcgta ggggtgccag
     2041 cggtaggaga agagggtctc gggctcgatc ctctccacgt cgatctcgaa gaccaggtgg
     2101 gtgtaaccgg gcgtcgtgat ctggcccttg agtctccgcc ccgcggcgaa gcccccctcg
     2161 aactttatcc cgaaccagga cccgaactcc tcggcgtcgg ctatggcctt ccagacccgg
     2221 gagcgccggg ccttgagtac gatgctcttc tctatgcggt cgctgttcat gtgcaacctc
     2281 ctggttgcga atatagcgag gcggggagtc gaacggcaac caaatagttg caggaagcgg
     2341 ggcttcggcc cgccaacaag cttcgaggca ctcgccgcgg gattgacgcg atgcgggcga
     2401 tcgacaagca tttgggcagg gatagcggcg accgcggccc gggtcgatcg gagggaggac
     2461 ggtgctcgaa cagaggctcg tggactggct cctcaactca gaccccgcac ttcggtggca
     2521 ggtcgagcgg gacctcctcg ggctgcccga ggccgtctgg ggcgcgacga gggcgcgcat
     2581 agccaccgaa ggcctcggga gcctcctcct ggagctccag gaccccgacg gtcagtgggc
     2641 gggcggggcc ttcttcccaa aggacttcga gttccaaggg cccgaggccg ccgagggcgc
     2701 gggccagccc tggaccgcga cgacctggag cctcaacacc ctcagggact ggggcctcga
     2761 tccgagcaaa ctcgcaggca ccgccgagcg cctcgctgcc aactgccgct gggagtacga
     2821 gggcctgccc tactggggcg gcgaggtgga ctgctgcatc aactcctgga ccctctccaa
     2881 cgggctctgg ctgggcgccg aggtcgacgg cctcgtggac tggttcctcg cccaccagct
     2941 cgccgacggg ggctggaact gcgagtgggt gaacggggcc acctgctcct ccttccactc
     3001 gaccctgaac gcgctgaagg gcctcctcga ctacgagcgc gccaccctcg accgcaaaag
     3061 gacagcccgg gcccggcgct cgggcgagga atacctcctc cagcggggcc tctacaggcg
     3121 gctctccacc gggatgcccg tcggcccctg ggccctcatc ttcgcctacc ccttccgctg
     3181 gttctacagc gtcctcaacg cggccgacta cttccgcgcc gcctcgatcc acgacggccg
     3241 cccccccgat ggcaggatgg cggaggcgat cgcgatcatc gagggggccc ggggcccgga
     3301 cggccggtgg
//
LOCUS       DASSQV010000064         6930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2381982, whole genome shotgun sequence.
ACCESSION   DASSQV010000064 DASSQV010000000
VERSION     DASSQV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381982"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFL04_01180"
     CDS             complement(<1..314)
                     /locus_tag="VFL04_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838339.1"
                     /translation="MSSDPEQSRLPAEALDAYLDGLLAELRDPSDPEALEEIRRAFRR
                     RVPFTLRSAAAAVLILRAAGFSRSPARPQAPKPSQGKARAAAKSETAKPGKKEKSGAA
                     AQ"
     gene            433..948
                     /locus_tag="VFL04_01185"
     CDS             433..948
                     /locus_tag="VFL04_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838340.1"
                     /translation="MSRRTPLPWLCIAAAVCMAPLEAQAGTETPAGAAAVSGQSAPIL
                     EDDPAPLLGYGIAEAITRFGPPGSVRPFRGEEAWQDDVAFIYPPGYTLFWSGDRLWQI
                     RFTAPYTGTIYGLFIGDQSSKVYSTLGQPYEIQGETLIYRLAYRGYPVRLSLVFASDR
                     LADVYLYRADY"
     gene            957..2453
                     /locus_tag="VFL04_01190"
     CDS             957..2453
                     /locus_tag="VFL04_01190"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET7838341.1"
                     /translation="MPLVCLSLTARTIFDNLAVLERYRSLVDMVELRADLLDPNEAFK
                     IRGFPELAALPCILSVRRKVDGGAFDAGEGVRLVVMAKALAYARNETGANFAYVELER
                     DFHVPAIEESCRTFGTRIIRSRHDMNGIPTDLDGAWAELSEIPDEIPKLAIMPQGAAE
                     LGSLLDWARRLPSGERIVVGMGDFGLPSRILAESIGSSIVYASALEAGLPGAAPGHLD
                     PATLENVYRFSEVHSDTTVYALGGGRSVAFSRSPQLHNAAFREAGFDSVYLPIPSEGI
                     TDFMSALEAARARGAAITVPFKEEVLSYLSYRSPEVEDIGACNTIVRSSGGWAGYDTD
                     AYGFERSLLEFLDRKDLHGLRVTVMGAGGAAKAVCYVLSRLGASALVLNRSLAAGKAL
                     ARRYGFAWGPSDDRAAELVADHADLIVQTTPVGMLGNVQGDPLEWYDLNGREAVYDVI
                     YRPDTTMLLERARAAGCRTTNGWAMLRYQAAEQFRLWTGAEPPSAYYR"
     gene            2462..3337
                     /gene="era"
                     /locus_tag="VFL04_01195"
     CDS             2462..3337
                     /gene="era"
                     /locus_tag="VFL04_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET7838342.1"
                     /translation="MPKSAFVAIAGRPSAGKSTLVNALCGEKVSIVSPVPQTTRNAVR
                     GIVNRSEGQLVLLDTPGFHISEKRLNKRLRDVAVDAFSDADIVIYLVDASRAAGSEEE
                     ALSAALASAAGRMVAVINKIDSPSANVAGARAFVAAHFPEAPLVEISALRLQGLDALL
                     AALYSRAAEGPAWYPEEYYTDQEPAFRIGEIIREKIMLHTRDELPHAVYVSYEDSRRS
                     GDGALHASYDLVVERDSQKGILIGKGGSMIKRIREEAEADLAGIFDYAVILKLSVRVD
                     PDWKKDEKRLRDLIF"
     gene            3351..4373
                     /gene="yiaK"
                     /locus_tag="VFL04_01200"
     CDS             3351..4373
                     /gene="yiaK"
                     /locus_tag="VFL04_01200"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF009750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HET7838343.1"
                     /translation="MGTIRIDFERMSATMAKALELRGFDPERAGRCARLFADASLDGV
                     YTHGLARFPRFIEYLQKGYVKATAVPRLEQSFGSWERWDGGLGPGNLNAAHAMARAIE
                     LSRGAGMGAVALRNTNHWMRGGSYGWQAAEAGCIGLAWTNTTPNLPAWGSTDCVLGNN
                     PLVIAVPRSGGHIVLDMAMSQFSYGKLEAYRDRGEELPVDGGFDSAGRPTRDPVEILA
                     SRRPMPIGYWKGSGLSMLLDLVGAVLSGGDSLAGLARREAEFGLTQFFLAFDLSRLPE
                     GDSGLRVAQELVAFVHSARPEREGGTVSYPGERTLRTREENLARGIPADEGLWAEVER
                     LASRGP"
     gene            complement(4357..4932)
                     /gene="pth"
                     /locus_tag="VFL04_01205"
     CDS             complement(4357..4932)
                     /gene="pth"
                     /locus_tag="VFL04_01205"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET7838344.1"
                     /translation="MIRLYAFLGNHGREYHGNRHNVAWLFLESLPFYDELRWERKFKG
                     RHAVRETPAGRTWILMPETFMNLSGDAVAELMRFHQIEPAEVLAVHDEIEMGFGLFGF
                     KMGGGLGGHNGLRSLEARLGTRDFARLRIGVGRPEHPDIADYVLSNFTKAERELLETS
                     VFPKAATALELCFAEGFETALAKYPKIRALD"
     gene            5024..6847
                     /locus_tag="VFL04_01210"
     CDS             5024..6847
                     /locus_tag="VFL04_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HET7838345.1"
                     /translation="MLVDRRFTLDVGEALGATRLSIHAAEELELREPMTRVASFDFDE
                     LCGFAGLDGKDREAFDEPGGPAIFSNGWQSWSFGGEVQGRETVRRARFIRKLNVYNEH
                     PATIRKRGELLSHFLTYFRAGDRRLFLVSCGSTEATLPPLCFRIDRSKLELALELYAE
                     GAAFKGGESLAEIAVFHRRGYFEAKDVLRGLFSGYGHFDRLAFLGRDGAIKPGGYESW
                     YNHYLDIDEGILARDIGSISDSPNLINRYYLSRGKPTVFQIDDGWERSVGEWLPDERR
                     FPRGMKVLAEAIEERGMVPGLWIAPLAVTRAAALHSEHPDWLLRDQAGSPVLAGWNPG
                     WGGDFHCLDISLPEVEDYILGVFDTMIEDWGYRYLKLDFLYAGMLRGKHARGGAAYRH
                     YDRLMHRITSRLSDSRGRPLAYLGCGAPLEPSFRHFPLMRIGADTREDWEYPSARLAR
                     HQGRPSAYVNLSHTIGKALLDGTVFVSDPDVMFCRSKGMGYGEREKELIAIVDRLLAS
                     QVMFSDDTHDFGSGGEAEFTSRIVGLFDRLADREYGVERIEADLYRLFSRDGRICGII
                     NLSDRERVAQLPCPAKAVVERARRRGTALAFEPRSISIYEG"
BASE COUNT         1164 a         2282 c         2270 g         1214 t
ORIGIN      
        1 ttctgggctg cggcccccga tttttccttc ttgcctggct tggccgtctc ggatttcgcc
       61 gccgctctgg ccttgccctg gcttggcttg ggggcctgag gtctcgcggg gcttcgggag
      121 aagcccgccg ccctgaggat cagcacggct gcggcagccg agcggagggt gaagggcacc
      181 cgcctgcgga aggctcttcg gatctcctcc agggcctcgg gatcggaggg gtctcggagc
      241 tcggcgagca ggccgtcgag gtaggcatcg agggcttcgg ccggcaggcg tgactgttct
      301 ggatcgctgg acacgtcgga ctcctttttt gtgcgtcgtg aggtattctg aacgatagcg
      361 cccatcatcg caagtcgggg caggcccgat tagatggaca gagccggggc cttcatatag
      421 agtcaaggcg aaatgagccg acgcacaccc cttccttggc tctgcatcgc cgcagcagtc
      481 tgcatggctc ccctcgaggc ccaggcgggg accgagactc cggccggagc tgccgccgtt
      541 tcaggacaga gcgcacctat ccttgaggat gacccggcac ccctccttgg ctatggaatc
      601 gccgaggcga tcacgagatt cgggcctcct ggctcggtca ggcccttccg cggcgaggag
      661 gcctggcagg acgacgtcgc cttcatctac cccccgggct acaccctttt ttggtccggt
      721 gaccggctct ggcagatcag gttcaccgcc ccctatacgg ggacgatcta tggcctattc
      781 ataggtgacc agagctcgaa ggtctactcc accttgggcc agccatacga gatccagggc
      841 gagaccctca tctaccggct cgcttaccgg gggtatccgg tcaggctgag cctggttttc
      901 gcttcggacc gccttgccga cgtctatctc taccgggccg actactagga gagaaaatgc
      961 cccttgtctg cctgtcgctg acggcgcgaa cgatctttga taacctcgcc gtcctcgagc
     1021 gctaccgcag cctcgtggac atggtcgagc tcagggccga cctcctcgac ccgaacgagg
     1081 ccttcaagat ccgaggtttt cccgagctgg ctgcccttcc ctgcatcctg tcggtacgga
     1141 ggaaggtgga cggcggggcc tttgacgctg gagagggggt caggctggtc gtgatggcca
     1201 aggccctcgc gtacgcgcgc aacgagacgg gggcgaactt cgcgtatgtc gaacttgagc
     1261 gggacttcca tgtccccgcg atcgaggaat cctgcaggac cttcggaacc aggatcatcc
     1321 gctcacgcca cgacatgaac gggatcccca ccgatctcga cggggcctgg gctgagctgt
     1381 ccgagatacc tgacgagatc ccgaaactcg ccatcatgcc ccagggcgcc gctgagctcg
     1441 gcagcctcct cgattgggcc cggagattgc catcagggga gcgcatcgta gtggggatgg
     1501 gcgactttgg ccttccgagc aggatcttgg cggagagcat cggctcatcg atcgtgtatg
     1561 cgagcgccct cgaggccggt ctcccagggg ctgccccggg ccacctcgac ccggccaccc
     1621 tggagaacgt ctacaggttc agcgaggtgc acagcgacac gaccgtctat gccctgggcg
     1681 gggggcggtc cgtcgccttc tcccgttctc cccagctgca caacgccgcc tttcgggagg
     1741 cgggtttcga ttccgtgtac cttccgatac cttcggaggg catcaccgac ttcatgtcgg
     1801 ccctcgaggc cgcgagggcc cgtggagccg ccatcacggt cccctttaag gaggaggtgc
     1861 tctcatacct gtcctaccgc tctccagagg tcgaggacat cggggcctgc aatacgatcg
     1921 tgcgctcctc cggcggctgg gcgggttatg ataccgacgc ctacggcttc gagcgcagcc
     1981 tcctcgagtt cctggacagg aaggacctgc acggcctcag ggtgacggtc atgggtgcgg
     2041 gcggggccgc gaaggccgtg tgctatgtcc tctcgcggct cggagcctcg gccctcgtgc
     2101 tcaaccggag cttggccgcg ggcaaggccc tggcgaggcg atacggcttc gcctgggggc
     2161 caagcgacga cagggcagcc gagctggtcg ctgaccatgc cgacctcatc gtccagacca
     2221 cgcccgtggg catgctcggc aatgtccagg gggaccccct cgagtggtat gacctgaacg
     2281 gcagggaggc ggtgtacgat gtcatctatc ggcccgacac caccatgctc cttgaaaggg
     2341 cccgggccgc gggctgcagg accaccaacg gctgggccat gctgagatac caggccgcag
     2401 agcaattccg actgtggacg ggcgccgagc cgccctccgc ctattaccgt taggagaaca
     2461 cgtgcccaaa tcagccttcg tagccatcgc cggccggcct tcggcaggca agtccacact
     2521 cgtgaacgcc ctctgcgggg aaaaggtgtc catcgtcagc ccggtgcccc agaccacgcg
     2581 gaacgcggtg cgcggcatcg tgaaccggag cgagggtcag ctcgtccttc tggatacccc
     2641 cggcttccat atttccgaga agcgcctcaa caaaaggctc cgtgatgtcg ccgtcgatgc
     2701 cttctcggat gcggacatcg tcatctatct ggtcgacgcg agccgcgcgg cgggctcgga
     2761 ggaggaggcc ctgtcggcgg ccttggcttc ggcggccgga cgcatggtcg ccgtgatcaa
     2821 caagatcgac tctccctccg ccaatgtggc tggggccagg gccttcgtgg ccgcccattt
     2881 ccccgaggcc ccgctggtcg agatctcggc gctcaggctc cagggcctcg atgccctcct
     2941 tgccgccctg tactcgcgcg ccgccgaggg tccggcctgg taccccgagg agtactacac
     3001 cgaccaggag ccggccttca ggatcggcga gatcatcagg gagaagatca tgctccacac
     3061 gagggacgag ctgccccacg cggtgtatgt cagctacgag gactcgaggc gctctggcga
     3121 cggcgccctg cacgcgagct acgacctcgt cgttgagagg gattcccaga agggcatact
     3181 gatcggtaag ggcggctcga tgatcaagcg catcagggag gaagccgagg ccgatctcgc
     3241 cggaatattc gattatgccg tcatcctgaa gctgtcggtc cgggtcgatc ccgattggaa
     3301 gaaggacgag aagcggcttc gagacctgat cttttagggc tgggggcggg atgggaacca
     3361 tcaggatcga ttttgagcgg atgtccgcga ccatggcgaa ggccctcgag ctccgcggct
     3421 ttgatccgga gcgcgccggg cgctgcgcga ggctcttcgc ggacgcgagc ctcgatgggg
     3481 tctacactca cggcctcgcg cgctttcccc gctttatcga atacctgcag aagggctacg
     3541 tcaaggccac agcggttccg aggctcgagc agagcttcgg ctcctgggag cgatgggacg
     3601 gtgggctcgg ccccggcaac ctcaatgcgg cccacgcgat ggccagggca atcgagctgt
     3661 cccgaggggc cggcatgggc gccgtcgccc tcaggaacac caatcactgg atgaggggcg
     3721 gcagctatgg ctggcaggcc gccgaggccg gctgcatcgg cttggcctgg acgaacacca
     3781 cgccgaacct gcccgcctgg ggctccacgg actgcgtgct cggcaacaat cccctggtca
     3841 tcgccgtgcc ccgctcaggc ggtcacatcg tcctcgacat ggcgatgagc cagttctcct
     3901 atgggaagct cgaggcctac cgcgacaggg gcgaagagct gcccgtcgat gggggcttcg
     3961 acagcgctgg gaggccgacg cgggatccag tcgagattct ggcctcacgc cgtccgatgc
     4021 ccatcggcta ctggaagggc tcggggctct ccatgctcct cgacctcgtg ggcgccgtgc
     4081 tctcgggcgg ggactcgctc gccggcctcg cccgccgcga ggcggagttc ggcctcaccc
     4141 agttcttcct cgccttcgac ctttcgaggc tccccgaagg cgattcaggc ctacgcgtcg
     4201 cccaggagct cgtggccttc gtccactccg ccagaccgga gcgggagggc ggcacggtca
     4261 gttaccccgg agagcggacc ctgaggacaa gggaagagaa cctcgcacgc ggcatacccg
     4321 ccgatgaggg gctctgggcc gaggtagagc ggctcgctag tcgagggccc tgatcttggg
     4381 gtatttcgcg agggccgtct cgaagccctc ggcgaagcag agctcgaggg ccgttgccgc
     4441 cttggggaat accgaagtct cgaggagctc gcgctcggcc ttggtgaaat tcgagagcac
     4501 ataatcggct atgtcggggt gctcggggcg gccgaccccg atgcggagac gggcgaaatc
     4561 cctcgtgccg agccttgcct cgagggatcg cagaccgttg tgtccgccca ggccccctcc
     4621 catcttgaag ccgaaaaggc cgaaacccat ttcgatctcg tcgtgcactg cgaggacctc
     4681 ggccggctcg atctggtgga aacgcatgag ctcggccacc gcgtcgcccg agaggttcat
     4741 gaaggtttcg ggcatgagga tccaggttcg tccggctggg gtctcgcgga cggcgtggcg
     4801 gcccttgaac ttcctctccc agcggagctc gtcgtagaag gggaggctct cgaggaagag
     4861 ccaggccacg ttgtgccggt tgccgtggta ttcgcggcca tggtttccaa ggaaggcata
     4921 gaggcgtatc agtgtggtgc tcccgggccc gtttgacggc cccggtcaag gatagtacat
     4981 taatcgccgc gatcctgcat atcgagcctc gaggaggagc cccatgttgg tcgatcggcg
     5041 cttcaccctc gacgtcggcg aggctctggg cgcaacaagg ctgagcatcc atgcggcgga
     5101 ggagctcgag ctgcgggagc ccatgacccg cgtcgcaagc ttcgacttcg acgagctctg
     5161 cggcttcgcc ggcctcgacg gcaaggacag ggaggccttc gacgagccag ggggccctgc
     5221 catcttctcc aacggttggc agtcctggtc cttcggaggc gaggtccaag ggcgggagac
     5281 ggtcaggcgc gcccgcttta tcaggaagct gaacgtctac aatgagcacc ccgcgaccat
     5341 caggaaacgc ggggagctcc tctcccattt tctcacctat tttcgcgcgg gcgatcgcag
     5401 gctcttcctg gtctcctgcg gctcgaccga ggcgacactc ccgcccctgt gtttccggat
     5461 cgacaggtcc aagctcgagc tcgcccttga gctctatgcc gagggggcag ccttcaaggg
     5521 gggcgagagc ctcgccgaga tcgcggtctt ccaccgcagg ggctacttcg aggccaagga
     5581 cgtccttcgc gggctcttca gcggctatgg ccatttcgac cggctcgcct tcctgggccg
     5641 ggacggagcc atcaagcccg gcggctacga atcctggtac aaccactatc tcgacatcga
     5701 tgagggcatc ctggcccgcg acatcggatc catatcggat agcccgaacc tcatcaaccg
     5761 gtactatctc tcaaggggca aacccacggt cttccagatc gacgacggct gggaacgcag
     5821 cgtgggcgag tggcttccgg acgagagaag gttcccgcga ggcatgaagg tcctggccga
     5881 ggccatcgag gaaaggggca tggtgccggg gctctggatc gcccccttgg ccgtgaccag
     5941 agcggccgcc ctccattctg agcatccgga ctggctcctc cgggaccagg ccggttctcc
     6001 cgtgctcgcc ggctggaacc ccggctgggg cggggatttc cactgcctcg acatttccct
     6061 ccccgaggtc gaggactaca tcctcggcgt gttcgacacg atgatcgagg actggggcta
     6121 ccgctacctc aagctcgact tcctctacgc gggcatgctc cggggcaagc acgcccgcgg
     6181 aggggccgcc taccgccact acgaccgcct catgcatcgc ataacgtcga ggctgtcgga
     6241 ctcgcgcgga aggcccctcg cctacctcgg ttgcggggcc cccctcgagc cttcgttccg
     6301 gcacttcccc ctcatgcgca tcggagccga cacgagggag gactgggaat acccgagcgc
     6361 gcggctcgcc cggcaccagg gcaggccctc ggcctacgtc aacctctccc acacgatcgg
     6421 aaaggccctg cttgacggca cggtcttcgt cagcgacccc gacgtcatgt tctgcaggtc
     6481 caaaggcatg ggctacggcg aaagggagaa ggagctcata gccatcgtcg accgcctcct
     6541 cgcatcgcag gtcatgttct cggacgacac ccacgatttc ggatcgggag gcgaggccga
     6601 gttcacctcg aggatcgtcg gcctgttcga ccgcctcgcg gaccgcgagt acggggtcga
     6661 gcggatcgag gccgaccttt accgcctgtt cagccgggac ggcaggatat gcggcatcat
     6721 caacctctcg gacagggagc gggtggccca gttgccctgc cccgcaaaag ccgtggtcga
     6781 gcgggcgcgc cgtcgcggga ccgccctcgc ctttgagcca cgatccatat ccatatacga
     6841 gggatgacca tggaaagaac cgtctacgtg atcgggcaca agaacccgga caccgacgcc
     6901 gtcgtggccg ccaaggccta cgccgagctc
//
LOCUS       DASSQV010000065         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1635175, whole genome shotgun sequence.
ACCESSION   DASSQV010000065 DASSQV010000000
VERSION     DASSQV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635175"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VFL04_01215"
     CDS             <1..977
                     /locus_tag="VFL04_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838346.1"
                     /translation="KTGGRGALVSVEDGSLGLDGCELSVAGEGDFVMLKAARSRLSVD
                     RSSLSSSRAMSFVAVEASASELEFRESVLSAKAGVRVFGAISMEGGSLTVSGSLLESS
                     ADLALTLLSLRGSSLLMDRTLVRAEAGTGYLRIGSFTDSTGELRSSKCIVSWKGAGTL
                     FESHGGSPSYRFDTIITETSSGLLRFFDADGRPPEVWNSILSNTAGSGELLRSTLPPT
                     AGSLVANCLWGFGSLVSGASAITSLAGLNALNAGSALYSSRPLLSEPPTATFAASVKS
                     LYPLSPGSACVGAALPLDERYSLDFGGGHRPSRSDGAMPDIGADQVSD"
     gene            988..2703
                     /locus_tag="VFL04_01220"
     CDS             988..2703
                     /locus_tag="VFL04_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838347.1"
                     /translation="MRRFSPLALLPLVLAALPLRAQDFSAYLGAGAGSRAASLLSAMD
                     AAAAALAKSRAEIRARMVKEEGGALLAYALDSASQGGENWALPDASLLGSGAAEAFAT
                     SRAKALALAEELAASTWGAELRAGKEAIGSRELAAAKLEALVRAAPDTEKALLGIERI
                     LKPGASLSRLFPEALELGSALRKAGREGRMLWLAALARRTAPEMAAYLSASREGIESL
                     SPASRETVDRLEAALAAYSGLIQALPVAAYPSELAVSALHKADTIGKAMGALLDLAPA
                     RSASLLGAMQKGDGLEAAAAAAAARFALIADALPAARRTMLQRAAFVSPSALARFSAL
                     AAPSPVPVPVPVPAPKPDPEPAGLEAQALELNGLAARFFAATGQDGTAKPGQAEEPAL
                     LLLERPELGSLAAGEARYAGLAAEARLRLARLYHAADETVRTALSRDARLLKTAAKAL
                     GAKPASLAVEAADLGSGKGGFGRRVTFVARATGPGGTAVLFPVAAELAAPAYASALAR
                     NAQIPAPASPAGGFLARYDQSVVSSLDLDDGGHFLLSALPRAGESLAHDYAEAEIRLL
                     EGWKP"
     gene            2700..>3560
                     /locus_tag="VFL04_01225"
     CDS             2700..>3560
                     /locus_tag="VFL04_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7838348.1"
                     /translation="MRAHPVLRPFLALALVCLVSCGQGRTGGGQGSGHGGQQGGAPRA
                     EPREAFSSSGRIAAEALRVDSLVAMTGGGLVPRTAPVSFDAGRLILFAAGKSGDKPAE
                     RLLAVDSEGKEGYTIALPAPGLALVAAGGQAIVSCADGSIMGIGFPPGAAGPVELWRR
                     SGAPADRLLALPEERFAEAAEDGSLAILSARSGDELWRTSLQGGASSLSYAPGLVLVG
                     VGSELAAYSEKDGIQAWRAQLGGPAAAASAGAGRAAVIDERGRVSFFDLADGRRLSEN
                     PGPFDPDIAPV"
BASE COUNT          440 a         1364 c         1237 g          519 t
ORIGIN      
        1 ccaagaccgg gggcagggga gccctggtct cggtcgagga cggctcgctc ggactcgacg
       61 gctgcgagct ctcggtcgcg ggcgaggggg atttcgtcat gctgaaggcc gcccgatccc
      121 gtctgtccgt tgaccggtcc agcctcagct cgagcagggc gatgagcttc gtcgccgtcg
      181 aggccagcgc ttccgagctc gagttcaggg aatccgtgct cagcgccaag gccggggtgc
      241 gcgtgttcgg ggccatcagc atggagggcg gctcgctcac ggtctcgggc tccctcctcg
      301 agagctcggc cgacctcgcc ctcaccctcc tctcccttcg cggctcgagt ctgctcatgg
      361 acaggaccct cgtccgcgcc gaggcgggga cgggctatct gcgcatcggc tccttcaccg
      421 acagcacggg cgagctcagg tccagcaagt gcatcgtgtc ctggaaggga gccggcaccc
      481 tcttcgagtc ccacggcggc tcgccatcct accgtttcga caccatcatc accgagacca
      541 gctcgggcct gctccgcttt ttcgacgccg acggccgtcc ccccgaggtc tggaactcga
      601 tcctcagcaa tacggccggc agcggcgagc tcctgcgctc gacccttccg ccgacagcgg
      661 ggagcctcgt ggccaactgc ctctggggct tcggcagcct cgtgagcgga gcgagcgcca
      721 taacgagcct ggccggcctg aacgccctca acgccggctc ggctctctac tcctcgaggc
      781 ccctcctgtc cgagccgccc accgcgacct tcgccgcctc ggtgaagagc ctctatcccc
      841 tcagcccggg ctcggcctgc gtcggggcag ccctgcccct ggacgagcgc tacagcctgg
      901 acttcggcgg cgggcacaga ccgagccgca gcgacggcgc gatgcccgac atcggcgccg
      961 accaggtctc ggactagggg gctgggcatg cgccgcttct cccccctcgc cctgctccct
     1021 cttgtcctcg cggccctccc cctgcgggcc caggatttca gcgcctatct tggggccggt
     1081 gccggttcca gggcggcttc cctgctgtcg gcgatggacg ccgcggccgc cgccctcgcg
     1141 aagagcaggg ccgagatcag ggcgaggatg gtcaaggagg agggcggggc actcctcgcc
     1201 tatgccctcg attcggcctc ccaggggggc gagaactggg ccctgcccga cgcctccctc
     1261 ctgggctcgg gggcggccga ggccttcgcc acctcgaggg ccaaggccct tgcccttgcc
     1321 gaggagctgg cggcctccac ctggggggcc gagctgcgcg cgggcaagga ggccatcggg
     1381 tcgagggagc tggcagcggc caagctcgag gccctcgtgc gagcggcccc cgacactgag
     1441 aaggccctgc tcgggatcga gaggatcctc aagccgggcg cctcgctttc gcggctcttc
     1501 cccgaggccc tcgagctcgg ctcggcgctc aggaaggccg gccgcgaggg gcggatgctc
     1561 tggctcgcgg ccctcgccag gaggaccgcc cccgagatgg ccgcctatct ctcggcgtcc
     1621 agggagggga tcgagtccct cagcccggcc tcgagggaga ccgtcgacag gctcgaggcg
     1681 gccctcgccg cgtattccgg cctcatccag gccctgcccg tggccgccta tccttcggag
     1741 ctcgccgtct cggcccttca caaggccgat acgataggca aggcgatggg ggccctcctc
     1801 gacctggccc cagcgcgctc ggcctccctc cttggggcga tgcagaaggg cgatgggctc
     1861 gaggccgcgg cggctgcagc ggcggctcgt ttcgccctca tcgccgacgc cctccccgcc
     1921 gcgcggagga cgatgctcca gcgggcggcc ttcgtctccc cttcggccct ggcccgcttc
     1981 tcggccctcg ccgccccctc gccggtcccg gtcccggtcc cggtgccggc gccgaagccc
     2041 gatccggagc ccgcggggct tgaggcccag gccctcgagc tgaatgggct cgccgcaagg
     2101 ttcttcgccg ccacgggtca ggatgggaca gccaagcccg gccaggccga ggagcccgcc
     2161 cttctgctgc tcgaacgccc cgagctcggg agcctcgccg ccggagaggc ccgctacgct
     2221 ggcctcgcgg ccgaggccag gctcaggctg gccaggctct accatgccgc cgacgagacg
     2281 gtccggacgg ccctctcccg cgatgcccgg cttctgaaga cggccgcgaa ggccctgggt
     2341 gcgaaaccgg cgtccctggc ggtcgaggcg gccgacctcg gctccggcaa ggggggcttc
     2401 ggccggcgcg tcacattcgt cgccagggcc acgggtccgg gagggactgc ggtcctcttc
     2461 cccgtcgccg ccgagctcgc ggcccctgcc tacgcctcag ccttggcgcg aaacgcccag
     2521 atcccagccc cggccagtcc ggcgggcggc ttcctcgcgc gctatgacca gagcgtcgtg
     2581 agctcgctcg acctggacga cggggggcat ttcctgctct ccgccctgcc aagggccggg
     2641 gagagcctcg cccacgacta tgctgaggcc gagatccgcc tcctcgaagg atggaagccg
     2701 tgagagcgca tccagtcctg cgccccttcc tcgccctcgc cctggtctgt ctcgtctcct
     2761 gcgggcaggg ccggacggga ggggggcagg gaagcggcca cggcgggcag cagggcggag
     2821 cgccccgagc cgagccccgc gaggcctttt cgagctccgg caggatcgcc gccgaagccc
     2881 tccgggtcga cagcctcgtc gcgatgaccg ggggcggcct tgtcccccga acagccccgg
     2941 tttccttcga cgcaggcagg ctgatccttt tcgccgcggg gaagagcggc gacaagccgg
     3001 cggagcgcct cctcgccgtc gactccgagg gcaaggaggg ctacaccatc gccctgccag
     3061 ccccaggcct cgccctggtg gccgcggggg gccaggccat cgtgtcatgc gcggacggct
     3121 cgatcatggg catcggcttc cctcccggcg cggcgggccc cgtcgagctc tggcggcgct
     3181 cgggggcccc cgccgaccgc ctcctcgccc ttccggagga gcgtttcgcc gaggcggccg
     3241 aggacgggag cctcgccatc ctctccgccc ggagcgggga cgagctctgg cgcacgagcc
     3301 tccagggcgg ggcctcgagc ctctcctacg cgccgggcct cgtccttgtc ggcgtgggct
     3361 cggagcttgc ggcctattcc gagaaggacg ggatccaggc ctggcgcgcc cagctcgggg
     3421 ggccagccgc ggccgcctcg gccggggcgg gcagggccgc cgtcatcgac gagcggggaa
     3481 gggtctcctt cttcgacctc gccgacgggc gcaggctctc cgagaaccct ggccccttcg
     3541 atcccgacat cgcccccgtg
//
LOCUS       DASSQV010000066         4151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1760548, whole genome shotgun sequence.
ACCESSION   DASSQV010000066 DASSQV010000000
VERSION     DASSQV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760548"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VFL04_01230"
     CDS             <1..752
                     /locus_tag="VFL04_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7838349.1"
                     /translation="SLGIPYYTHESDLSPGLATRLNARRAEGIVISYEETRGRLPPAL
                     RAKARLAGNPIRRAIRRGDRDTGRAWLGVGDGLPVVLFLGGSQGARQLNELVEAILPA
                     LGGRAFVAHQAGPGSEPISEPGYRRFGFISREMPEVLAAADIVVGRSGAGTLWECGAL
                     GKPMILIPLQGSGTRGDQVENAELFAKAGAAISLSGKGLGPEALLGEIGGLLDDRNRA
                     SALGAAASRLAGTDAGDLVADLILGRIGARK"
     gene            749..1243
                     /locus_tag="VFL04_01235"
     CDS             749..1243
                     /locus_tag="VFL04_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET7838350.1"
                     /translation="MNTMDWIFVGLIVILAIRCFVRGFVEEILSVASIVGGIFAALLL
                     YRPAGAFLKTRLGVSLVPEVLGFLVVFLLTFLAVKLLERLLTEGLEASRLERADRIVG
                     LFLGMIEGLLLVSVLLVIMSIVHIPGLQKALEGSAFAKLILPIIGPEVTKALTPAFNA
                     IKKP"
     gene            1251..2537
                     /locus_tag="VFL04_01240"
     CDS             1251..2537
                     /locus_tag="VFL04_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838351.1"
                     /translation="MFENLLAQDEALALLRSDVETGRLPPALLFAGPPGSGKLTAALE
                     MARALSCTAPGERAAWNCPCPSCARHRSLSHSDLVLMGPRTFPEELGAALEMLERSPG
                     KAASYFFVRAARKLLRRFDPVLFEGEEARLGKAIALVREAEENLERLAPERASTGQLA
                     PGAAEAAAKVAGACAKLEGFVPDSLPVFMVRNAELWARLAPLGSRKTVVIENADRMLD
                     SARNALLKILEEPPESVRFVLLSSRRSALLGTILSRSRTYAFRARDAAGTQLVLDRVF
                     RSGEPAASVEAFLAARRAFPPSEARQRAEAFLSAALAARTERSSLPTPLAEMARTAEA
                     ERRSPSLALAALMEATKDFGAKDERYASSFRSFLSALAGILGAALRDGEMDARGMETV
                     ESWARLLREARADYESWNRNPGLIAETLLYAIAEEP"
     gene            2534..3742
                     /locus_tag="VFL04_01245"
     CDS             2534..3742
                     /locus_tag="VFL04_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7838352.1"
                     /translation="MRRFIERAMAKVPRMSEDQMRALLYALAEENGRMEVALDSMLDG
                     IVVCDNDHLPIIYNKTAERMLRLGGPDPSERPLWLAVEDEELARFLKATLEGEDSVLD
                     REFRLESKGSVRLVAASVTPLVSRGRIQGALLHLEDITEKRKREAQLRRAESLASLTT
                     LAAGVAHEIKNPLGAISIHIQLIKKAALGKAGLDPAILERHLGVVDEEIERLNKIVVD
                     FLFAVRPMDVQLIETDPGELVREIADFMGPEAERAGVSLELELDEGLPRVLLDRRYMK
                     QAILNLVKNALAAMPGGGRLVLSARAGEDGVEISVEDSGVGIAEEDLAKIFEPYFTTR
                     ENGTGLGLTITFKIVKEHRGEISVSSRQGQGSIFTISLPVPQKVQRLLAWDEDPSLGE
                     EAGDTRKAEA"
     gene            3759..>4151
                     /locus_tag="VFL04_01250"
     CDS             3759..>4151
                     /locus_tag="VFL04_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7838353.1"
                     /translation="MRFTVLVADDEKNIREGLAEALGLEGYRTLGASDGDEALKLVEQ
                     GDVDLVITDLKMPKVAGTEVLKTVVGRYPGLPVIVLTGHGTIEDAVTAMRSGAFDFVT
                     KPVNLDHLSLLVKRALESRELARKNRELE"
BASE COUNT          637 a         1546 c         1369 g          599 t
ORIGIN      
        1 cctcgctggg catcccctat tacacccacg agtcggacct gagccctggc ctggccacga
       61 ggctcaacgc cagaagggcc gagggcatcg tgatcagcta cgaggagacc cgcggccgtc
      121 tgccccccgc cctgagggcc aaggccaggc tcgccggcaa cccgatacgc agggccatca
      181 gacggggcga ccgcgacacg gggagggcct ggctcggggt cggggacggg ctgcccgtcg
      241 tcctcttcct gggcggcagc cagggggccc gccagctcaa cgagctggtc gaggccatcc
      301 tgcccgccct tggcggcagg gccttcgtcg cccaccaggc cgggcccggg tcagagccca
      361 tatccgagcc cggctaccgc cgtttcggct tcatatcccg cgagatgccc gaggtcctcg
      421 ctgcggccga catcgtggtg ggccgctcgg gggccggcac cctctgggaa tgcggagccc
      481 tcggcaaacc catgatcctc atccccctcc agggctcggg cacgcgcggc gaccaggtag
      541 agaacgccga gctcttcgcg aaggcggggg cggccatcag cctctcgggc aaggggctcg
      601 gccccgaggc cctcctcggc gaaatcggag ggctcctcga cgaccggaac agggcctcgg
      661 ccctcggcgc ggccgcgtcg aggctggcgg ggaccgatgc aggcgacctc gtcgcagatc
      721 tcatcctggg caggataggg gcacgaaaat gaacaccatg gactggatct tcgtcggcct
      781 catcgtcatc ctcgcgatac gctgcttcgt caggggcttc gtcgaggaga tcctctcggt
      841 cgcctccatc gtcggtggga tcttcgccgc cctcctcctc tacaggccgg cgggcgcatt
      901 cctcaagacc cggctcggcg tgagcctcgt gcccgaggtc ctcggcttcc tcgtcgtctt
      961 cctcctcacc ttcctcgccg tgaagctcct cgagcggctt ctgaccgagg gcctcgaggc
     1021 ctcccggctc gagcgcgccg acaggatcgt ggggctcttc ctcggcatga tcgagggcct
     1081 cctcctcgtg tccgtcctcc tggtcatcat gtcgatcgtc cacatcccag gcctgcagaa
     1141 ggccctcgag ggctcggcct tcgcgaagct gatcctgccg atcatcggcc ccgaggtcac
     1201 gaaggccctg acccccgcct tcaacgccat aaagaagccc tgataggacc atgttcgaga
     1261 acctgctggc ccaggacgag gccctcgccc tcctgcgctc cgacgtcgag accgggcgcc
     1321 tcccgcccgc gcttctcttc gccggccctc cgggctcggg caagctgacg gccgccctcg
     1381 agatggcgcg ggccctctcg tgcacggccc cgggcgagcg ggcggcgtgg aactgccctt
     1441 gcccctcctg cgcccgccac cggtccctga gccactccga cctcgtcctc atggggccga
     1501 ggaccttccc cgaggagctc ggcgccgcgc tcgagatgct ggagcgctct cccggcaagg
     1561 ccgcctcgta cttcttcgtg agggcggcgc ggaagctcct gcggcgcttc gaccccgtcc
     1621 tcttcgaggg cgaggaggcc aggctcggca aggccatcgc cctcgtccgc gaggccgagg
     1681 agaacctcga gcggctcgcc cccgagaggg cctccactgg ccagctcgcc cccggcgccg
     1741 ccgaggcggc ggccaaggtg gcaggagcct gcgccaagct cgagggcttc gtgcccgact
     1801 ccctgcccgt cttcatggtc cgcaacgccg agctctgggc gaggctcgcc cccttaggct
     1861 cgcgcaagac cgtggtcatc gagaacgccg accgcatgct cgattcggcg cgaaacgccc
     1921 tgctcaagat cctcgaggag ccgccggaga gcgtgcgctt cgtcctcctg agctcgagac
     1981 ggagcgccct cctcgggacc atcctctcgc gctcgaggac ctacgccttc agggccaggg
     2041 acgccgcggg cacccagctc gtcctggacc gcgtcttccg ctcgggcgag cccgcggcct
     2101 cggtggaggc cttcctcgcc gccaggcggg ccttcccccc gagcgaggcg aggcagaggg
     2161 ccgaggcctt cctctcggcg gccctcgccg cccgcacgga gcgctcctcc ctgccaaccc
     2221 ccttggccga gatggccagg acggccgagg cggagaggcg gagcccctcc ctcgccctgg
     2281 ccgccctcat ggaggccacg aaggacttcg gcgccaagga cgagcgctac gcctcgagct
     2341 tccgctcctt cctctcggcc ctggccggca tcctcggcgc cgcgctgcgc gacggggaga
     2401 tggatgcccg cggcatggag accgtggagt cctgggcgag gctcctccgc gaggcgaggg
     2461 ccgactacga gtcctggaac cgcaaccccg gcctcatcgc cgagaccctc ctctacgcca
     2521 tcgcggagga gccatgaggc gcttcataga gcgggccatg gccaaggtcc cccgcatgag
     2581 cgaggaccag atgcgcgccc tcctgtacgc cctcgccgag gagaacgggc gcatggaggt
     2641 cgccctcgac tcgatgctcg acgggatcgt ggtctgcgac aacgaccacc tgccgatcat
     2701 ctacaacaag acggccgagc gcatgctccg cctcggcggc cccgacccct ccgagcgccc
     2761 gctctggctg gccgtggagg acgaggagct ggcccgcttc ctcaaggcca ccctcgaggg
     2821 cgaggactcg gtgctcgacc gcgagttcag gctcgagtcc aagggctcgg tccgcctcgt
     2881 cgccgcatcg gtgacccccc tcgtgtcccg gggcaggatc cagggcgccc tcctccacct
     2941 cgaggacatc accgagaagc gcaaacgcga ggcccagctc aggcgggccg agagcctggc
     3001 ctcgctcacc accctcgccg cgggcgtggc ccacgagatc aagaaccccc tcggcgcgat
     3061 ctcgatccac atccagctca tcaagaaggc cgccctcggg aaggctgggc tcgatcccgc
     3121 catcctcgag cgccacctcg gcgtcgtgga cgaggagatc gagcgcctca acaagatcgt
     3181 cgtggacttc ctcttcgcgg tcaggcccat ggacgtccag ctgatcgaga ccgaccccgg
     3241 cgagctcgtg agggagatcg cggacttcat gggccccgag gccgagcgcg ctggcgtgtc
     3301 cctcgagctc gagctcgacg agggcctgcc ccgcgtcctc ctcgaccggc gctacatgaa
     3361 acaggccatc ctcaacctcg tgaagaacgc cctcgcggcg atgcccggcg gcggaaggct
     3421 cgtgctctcg gcgcgcgccg gggaggacgg ggtggagatc tcggtcgagg actcgggcgt
     3481 cgggatcgcc gaggaggacc tcgcgaagat cttcgagccc tacttcacga cgagggagaa
     3541 cggcacgggg ctcgggctca ccatcacctt caagatcgtc aaggagcaca ggggcgagat
     3601 ctcggtctca tcgcggcagg gccagggctc gatcttcacg atcagcctcc cggtcccgca
     3661 gaaggtgcag aggctcctgg cctgggacga ggacccgagc ctcggggaag aggccggcga
     3721 cacgaggaag gccgaggcct aaaggggtgc aggaagcgat gcgtttcacg gtgcttgtgg
     3781 ccgacgacga gaagaacatc agggagggcc tggccgaggc ccttggcctc gagggataca
     3841 ggaccctcgg cgcctcggac ggggacgagg ccctgaagct cgtcgagcag ggcgacgtcg
     3901 acctcgtgat caccgacctc aagatgccga aggtcgccgg caccgaggtc ctcaagaccg
     3961 tcgtggggcg ctaccccggc ctccccgtca tcgtcctcac cggccacggg acgatagagg
     4021 acgccgtcac ggccatgcgc tcgggggcct tcgacttcgt gaccaagccg gtcaacctcg
     4081 accacctctc cctcctcgtc aaacgcgccc tcgagagccg tgagctcgcg cgcaagaacc
     4141 gcgagctcga g
//
LOCUS       DASSQV010000067         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_19213, whole genome shotgun sequence.
ACCESSION   DASSQV010000067 DASSQV010000000
VERSION     DASSQV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19213"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFL04_01255"
     CDS             complement(<1..346)
                     /locus_tag="VFL04_01255"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7838354.1"
                     /translation="MKDAATMRAERREIFGWAMYDWSNSAFSTTIGTVFLGPYLASLA
                     AEAASRAPDGLARLLGLPIAPDSFMPYCVSLSVGLQVLFLPLLGAIADYSRLRKGMLR
                     LFATLGALATIAL"
     gene            complement(375..1664)
                     /locus_tag="VFL04_01260"
     CDS             complement(375..1664)
                     /locus_tag="VFL04_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HET7838355.1"
                     /translation="MGTKPSRRLVVVSIDGLSLREAEAIAGLPRFSSLMARGAFSSSL
                     RSVFPSLTYVVHATMMTGRHPVAHGVTHNHPLQPGLPADRQAWFWYADQLRVPSLFDL
                     ARKAGMRTAAVLWPLVSGARITWNLPEIAALPGENQTIKALRAGSPAYLLALEARFGK
                     FRRGAEQPFLDDFVSRSAAHTIRSRRPDLLMTHLIALDAAKHEAGSDSPAARSALEFL
                     DLALGRIIDALGDSGLADSTDLVVLGDHGHIDIHHRIRPNRLLQDAGLCGHRDGAFGW
                     RAWCRCSGGSAFVHTRPGDAGAEALVRSALEAAAADPAMGIEAILDRGGIAALHGDGD
                     ASFALLARPGFQFLEEFGGPLAETESRPGAFGADHGYSPELPGYRSLLLAAGPGIRSG
                     RLAGEIEMVDVAPSLARLMGLEFPAGDGAPIEGLFQD"
     gene            1746..>2484
                     /locus_tag="VFL04_01265"
     CDS             1746..>2484
                     /locus_tag="VFL04_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7838356.1"
                     /translation="MHYRRFPLIEDREISALGLGCMRLPTLGGDDAAIDEAAFDAMLA
                     AAADAGVTYLDTAYVYHRGKSETALGAALERTGLKGRFSLATKSPVWMVKEAGDWDRF
                     LNEQLERLRTGHIDFYLLHALNAERWEKVQSLGGIGALERARADGRIGHIGFSFHDSL
                     DSFKRIVDGYNGWEFCQVQFNYVDLDFQAGAAGIEYAAAHDIGVIVMEPLRGGGLARP
                     PADVGAELRRYPKPRTPAEWALRFALDR"
BASE COUNT          385 a          832 c          919 g          348 t
ORIGIN      
        1 agagggctat cgtggcgagg gcgcccaagg tggcgaagag cctgagcatg cccttgcgca
       61 gccgggaata gtcggcgatc gcgcccagga ggggcaggaa gaggacctgg aggcccacag
      121 agagcgagac gcagtagggc atgaaggagt cgggggcgat ggggaggccg aggagccggg
      181 ctagcccgtc gggggccctg ctggcggcct cggccgcgag ggaggcgagg taggggccga
      241 ggaagacggt cccgatcgtc gtgctgaagg cgctgttgga ccagtcgtac atggcccagc
      301 cgaagatctc cctgcgctcg gcgcgcatcg tcgccgcgtc tttcatcgtc ccccctcgtg
      361 ccccgtggca gccctcagtc ctggaaaagc ccctctatcg gggcgccgtc gcccgctgga
      421 aactcgaggc ccatgagcct cgcgaggctc ggcgccacgt ccaccatctc gatctccccc
      481 gcaagccttc ccgacctgat gcccggaccc gcggccagaa ggaggctgcg gtagcccggc
      541 agctcggggc tgtagccgtg gtcggccccg aaggccccgg gccggctctc ggtctcggcc
      601 agggggccgc cgaactcctc gaggaactgg aagccggggc gggccaggag ggcgaagctg
      661 gcatccccgt cgccgtggag ggctgcgatg ccgccgcggt cgaggatcgc ctctatgccc
      721 atggcggggt cggcggcggc cgcctccagc gcagagcgca caagggcctc ggcaccggcg
      781 tccccaggcc gcgtatggac gaaggccgag ccgcccgagc agcggcacca ggccctccag
      841 ccgaaggccc cgtccctgtg gccgcagagc ccggcgtcct ggaggaggcg gttgggccgg
      901 atgcggtggt ggatgtcgat gtgtccatgg tcgccgagga cgacgaggtc tgtcgagtcg
      961 gcgaggccgg agtccccgag cgcgtcgatg atcctgccga gggcaaggtc gaggaactca
     1021 agcgccgagc gcgcggcggg tgagtccgaa cccgcctcgt gcttcgccgc gtcgagtgcg
     1081 atgaggtggg tcatgaggag gtcgggcctg cggctccgta tggtgtgggc ggcgcttcgc
     1141 gagacgaagt catcgaggaa gggctgttcg gccccccttc tgaatttgcc gaagcgggcc
     1201 tcgagggcga ggaggtaggc ggggctgccg gccctgaggg ccttgatcgt ctggttctcc
     1261 ccgggcaggg ctgcgatctc gggcaggttc caggtgatcc tcgccccgga gacgaggggc
     1321 cagagcacgg cggccgtcct catgccggcc ttcctggcca ggtcgaagag gctggggacg
     1381 cgcagctggt ccgcgtacca gaaccatgcc tggcgatccg cgggaaggcc gggctggagg
     1441 gggtggttgt gggtcacgcc atgggccacg gggtgcctcc ccgtcatcat cgtggcgtgg
     1501 acgacgtagg tgaggctcgg gaacacgctc cggagcgagg acgagaaggc gcctctcgcc
     1561 atgagggagg agaagcgggg caggccggcg atcgcctcag cctcccgcag ggagagcccg
     1621 tcgatcgaga cgacgacgag gcggcgcgat ggcttcgtgc ccatgggcga gagtatacac
     1681 gcagacgggg agcggcgctt cccagaacgc ttcccgaagg ccgggccctc ctgtatagtt
     1741 ggcccatgca ctaccgacga ttccccctca tcgaagaccg cgagatctcg gccctcggcc
     1801 tcggctgcat gcgcctgccc accctgggcg gcgacgatgc cgccatcgac gaggccgcat
     1861 tcgacgccat gctggccgcc gctgccgatg cgggcgtcac ctacctcgac acagcctacg
     1921 tgtaccaccg cggcaagagc gagacggccc tcggcgccgc cctcgagcgc acgggcctga
     1981 agggccgctt cagcctcgcc acgaagagcc ccgtctggat ggtcaaggag gccggggact
     2041 gggaccgctt cctgaacgag cagctcgagc gtctgcgcac cggccacatc gatttctacc
     2101 tcctccacgc cctgaacgcc gagcgctggg agaaggtcca gagcctgggc gggatcgggg
     2161 ccctcgagcg cgcaagggcc gacgggcgca tcggccatat cggcttctcc ttccacgatt
     2221 ccctcgactc cttcaagagg atcgtcgacg gctacaatgg ctgggagttc tgccaggtcc
     2281 agttcaacta cgtcgacctc gacttccagg ctggggccgc ggggatcgag tacgcggccg
     2341 cgcacgatat cggcgtcatt gtcatggagc cgctgcgcgg gggcggcctc gcccggccgc
     2401 ccgccgacgt gggcgccgag ctcaggaggt accccaagcc gcggactccg gcggaatggg
     2461 ccctccgctt cgccctcgac cgcc
//
LOCUS       DASSQV010000068         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1884220, whole genome shotgun sequence.
ACCESSION   DASSQV010000068 DASSQV010000000
VERSION     DASSQV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884220"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VFL04_01270"
     CDS             complement(<1..972)
                     /locus_tag="VFL04_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7838357.1"
                     /translation="MALIEIKDALFCYEGGERPAVEGLCLSVEAGDYLAIVGPNGSGK
                     STLLRLLNGLLAPQSGSVLVDGLDASDPRNARAVRSALTLVFQSPPDQIVSTVVEEDV
                     AFGPENLGLARAEIGRRVSRALEATGLTDERCSPPHFLSAGQQQRLAVAGAIAMEPKA
                     IAFDEATSMLDPAGRDSILSLIDGLVAKGITVIHVTHDMSEAARARRIVAMVSGRVAF
                     DGSPSELWGGEGGPGQGELPIASLGLPASVAAGRRLGVRVGAADSPALIASLVAAERE
                     AGRAVAHAGTVTREQVTGHARRLPATGGEAAFALSGVSYSYLRGTSNE"
     gene            complement(1293..2135)
                     /locus_tag="VFL04_01275"
     CDS             complement(1293..2135)
                     /locus_tag="VFL04_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET7838358.1"
                     /translation="MRALGCRAHDFGRLGAEELGSRVSAAGFTCVQLAPAKAIEGIKH
                     RPGALDPARAAAISASLAARGVRIAVLGCYINPIHPDPEERESSIRRFAESLALARVF
                     GCGIVATETGSLNADCSFHPGNSGKAAFSELVGSLETIAVEAERHGVYACIEGVASHV
                     ASSPARLAAILEAVPSPWLRVLFDPVNLLTASTWGEQGRMFEEAESLLGGRILAFHAK
                     DFQIEGVVSRVCPPCRGLLDYGRFLDMAERVAPDADVLIEDLRPADMAGAREFLLSRL
                     GGRG"
     gene            complement(2132..>2592)
                     /locus_tag="VFL04_01280"
     CDS             complement(2132..>2592)
                     /locus_tag="VFL04_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838359.1"
                     /translation="GAGVVVGHLGLDYHADHRAAAAITEAAALLVTLPNIPSRYPALR
                     STPLLYHSSTLGLTDPLGNSLPRPHFFVDVGSVWETKAAMLSCHRSQEEVMRVQHGVG
                     DFLGMMRRQDETWGREAGVARAEAFWQHLGGGFAKTPLIQTELAAFVVKG"
BASE COUNT          399 a          850 c          955 g          388 t
ORIGIN      
        1 ctcgttcgag gtgccccgca gataggagta ggaaaccccg ctcagggcga aggcggcctc
       61 gccgccagtg gcgggcaagc gccgcgcgtg gccggtgacc tgctcgcggg tgactgtccc
      121 cgcgtgggcg actgcccggc ccgcctcccg ctctgcggcg acgagggagg cgataagggc
      181 ggggctgtcg gctgccccga ccctgacacc gagacgccga ccagcggcta cggaggcggg
      241 aaggcccagg gaggcgatgg gaagctcccc ttggcccggg ccgccctccc cgccccagag
      301 ctcggagggg ctcccgtcga aggcgactcg tcccgacacc atcgcgacga tgcgtcgggc
      361 cctcgccgcc tccgacatgt cgtgggtgac gtggatgacg gtgatgccct tcgccacgag
      421 tccgtcgatg aggctgagga tcgagtcgcg ccccgcggga tcgagcatcg aggtggcctc
      481 gtcgaaggcg attgccttcg gctccatggc gatggccccc gcgacggcga gccgctgctg
      541 ttggccagcc gagaggaaat gtggcggact gcatcgctcg tcggtgagcc cggtcgcctc
      601 gagggccctg ctgacccgcc tcccgatctc tgccctcgcg agcccgaggt tctcggggcc
      661 gaaggcgacg tcctcctcga cgacggtcga gacgatctgg tcgggcgggg actggaagac
      721 gagggtgagg gctgagcgga cggcgcgcgc attgcgcgga tccgaggcgt cgaggccatc
      781 gacgaggacc gacccggact gcggggcgag aaggccgttg aggaggcgaa gcagggtgct
      841 cttgcccgat ccgttcgggc caacgattgc gaggtagtcc cctgcctcga ccgagaggca
      901 gaggccttcg accgccgggc gctcccctcc ctcatagcag aagagggcgt ccttgatctc
      961 tatgagcgcc atcggatctc ccgggagcag ggagatgggc ggaagcagtt catgttcacc
     1021 tcgcgtctcg gcggctcagc cagccgtcca agcgacgccg tgaatttaga caaagcagcc
     1081 cgcggtgtca agctggcgca tgccggcagg cttgggcgga tttggggtga ccggatttgg
     1141 ggtgactgtc cccattgctt cctcgcgggg gtgactgtcc ccatagcgtc tagtggtgac
     1201 tgtccccgta gggtgacggt gactgtcccc atagcgtcct agtggtgact gtccccgtag
     1261 gtgtgactgt ccctgtagcg tcccgcggct tctcagccgc gaccgccgag acgcgagagc
     1321 aggaactcgc gcgcccccgc catgtcggcc ggcctgagat cctcgatcag gacatcggcg
     1381 tcaggtgcaa cccgctccgc catgtcgagg aaacggccgt agtcgaggag gccgcggcag
     1441 ggggggcaga ccctgctcac cacgccctcg atctggaagt ccttcgcgtg gaaggcgagg
     1501 atgcgccctc ccaggagtga ctcggcctcc tcgaacatcc tcccctgctc accccaggtc
     1561 gaggccgtga ggagattgac ggggtcgaag aggaccctga gccagggcga gggcaccgcc
     1621 tcgaggatag cggccagcct ggcagggctc gaggccacgt ggcttgccac gccctcgatg
     1681 caggcgtaga ccccgtggcg ctcggcctca acggcgatcg tctcgaggct cccaacgagc
     1741 tcggagaagg ccgcctttcc cgagttgccg gggtggaatg agcagtcggc gttgagggag
     1801 cccgtctcgg tggcaacgat gccgcatccg aagacgcgcg cgagggctag gctctcggcg
     1861 aagcgcctga tcgagctctc cctctcctcc ggatcggggt ggatcgggtt gatgtagcag
     1921 ccgagcacgg cgatccggac cccccgggcg gccagtgaag ccgagatggc tgcggccctg
     1981 gccggatcaa gggcccccgg acggtgcttg atgccctcga tggccttggc gggggcgagc
     2041 tggacgcagg tgaagcccgc cgcagagacc ctcgatccga gctcttcggc cccgagcctg
     2101 ccgaagtcgt gggccctgca gccgagggcc ctcaaccctt caccacgaag gcggcgagct
     2161 cggtctggat gaggggggtc ttcgcgaagc cgccgccgag gtgctgccag aaggcctcgg
     2221 ctcgagcgac tccggcctcc ctgccccagg tctcgtcctg gcgccgcatc atgccgagga
     2281 agtccccgac cccgtgctgg acacgcatta cctcctcctg gctccggtgg caggagagca
     2341 tcgcggcctt ggtctcccag accgagccca cgtcgacgaa gaagtggggc cggggcaagc
     2401 tgttcccgag gggatcggtg aggccgaggg tcgaggaatg gtagagcagg ggcgtggagc
     2461 gcagggccgg gtagcgggag gggatgttgg ggagggtcac gaggagggcg gcggcctcgg
     2521 tgatcgccgc cgcggctcgg tggtcggcgt ggtagtcgag gccgaggtgg ccaaccacga
     2581 cgccggcccc gg
//
LOCUS       DASSQV010000069         4250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1138441, whole genome shotgun sequence.
ACCESSION   DASSQV010000069 DASSQV010000000
VERSION     DASSQV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138441"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFL04_01285"
     CDS             <1..563
                     /locus_tag="VFL04_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HET7838360.1"
                     /translation="IKIASEVFDLEVDKKKKKLVLLEWPKLKAIRDQLDRETFGLYKS
                     VKGVVRIGHQRLLDGEKLDVILRIASWLDPERDLAREWPRKRNFDAGEGLHELLSSLD
                     HVLQIARARDRTAELGFVTLYTDAEGMFWFRCSRGFHTALSESLASLEALADRIGGDG
                     AEGAAASPEERERVNSLYRKLSSFFD"
     gene            698..1423
                     /locus_tag="VFL04_01290"
     CDS             698..1423
                     /locus_tag="VFL04_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838361.1"
                     /translation="MKDGPLVLGLELGIGAESETGLPWAVEPSLALDADWDRYYARIE
                     LSQAFGELDWQRVLGYRVLDTAGMAVGWRPELADRLDLRLEAFSRYRSFGGAGYEARF
                     GALALLQAGAMRGEAGPFFIAGAGYSRLMTRYSGTAVSQWDNDPLARIGFGWRDGRRL
                     VLDMAISDYTGADASLWLKTFFEFGGRLAFGEGEAPEARATLVLKYSDFFTLTSYLDG
                     FALRLSMSLPLGSPAWSAGLTGI"
     gene            1420..2322
                     /locus_tag="VFL04_01295"
     CDS             1420..2322
                     /locus_tag="VFL04_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7838362.1"
                     /translation="MRKAHAALGIAALALVLASCSYGILEGFGRPDPVGERIDEPGQR
                     SSFATDKTLVEGNLTAGTAFTFIVSSDVHFGYKGGPRQAALSDFADLAKTSNADFALF
                     GGDDADKGSEGEYQDFSAFADGLAPSAATQLLLGTTRLPWFSAVGNHDLYNSGWGLFR
                     KYVGPSYYVLSVGSVGIYVIDTGNGTLGEKQLERLGAAMSADPRPKIVLSHYPIYGSD
                     RFIYYRITNPRERIELIDLFARTRVVAVFVGHWHYPEESDVGPFIERVSGSLVDSDID
                     GKAHAWVVSVDATGTQVTATRHDF"
     gene            complement(2348..3022)
                     /gene="trmB"
                     /locus_tag="VFL04_01300"
     CDS             complement(2348..3022)
                     /gene="trmB"
                     /locus_tag="VFL04_01300"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET7838363.1"
                     /translation="MSGDTSIRTYVLRTGRMTDAQRRALEELGPRYLLPFHPGQRLDY
                     TSAFGDRRPVVAEIGFGMGQATWRIALENPETGYLGIEVHTPGIGKLLIDIAEHDVRN
                     IRIIHHDAVEVLERMIAPASLAGLHIFYPDPWPKKKHHKRRLIREGLAELLASRLAPG
                     AYLYFVTDIEEYAQWSLELLSRTPGLRNRHEGYAPKQAWRPETKFEARAKGDGRAVWE
                     LLFERS"
     gene            complement(3019..3693)
                     /locus_tag="VFL04_01305"
     CDS             complement(3019..3693)
                     /locus_tag="VFL04_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838364.1"
                     /translation="MPELHRGRAFALAALALLASSLIGACGGKPPELAAVEWRLEQRQ
                     SKAGPYESLWVFASVKDSEGIDDLDALYVVNDAAGIDWRLTNANWTKRTEGGDTWIGA
                     ADLALNDYGPLPRGGYRAMAMTAAGERAERAFSVEGQFPDYAAPRIEYSKGQVSLRSA
                     WPETLIIGLDGTGAVLGSSSWTGAAESLGDHFGVDIAARVAAIQAYGYEPSRNMGSYS
                     ERVVVK"
     gene            complement(3693..>4250)
                     /locus_tag="VFL04_01310"
     CDS             complement(3693..>4250)
                     /locus_tag="VFL04_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HET7838365.1"
                     /translation="ASVEGAGLADRLALTPSITRGLDYYTGVVFETSLDSIPTIGSVC
                     SGGRYDELASLYTSRRLPGVGASIGLDRLLAALEAIGASRRKPGYTQVLVLDLEESLA
                     PRYQAMARDCRAAGLACEIFPEKRKLAQQFAYAERKGIPFALVLGSAEAERGAIAVKD
                     LRTRESVELASIPAVIGLLRERLGP"
BASE COUNT          655 a         1455 c         1425 g          715 t
ORIGIN      
        1 agatcaagat cgcctcggag gtcttcgacc tcgaggtgga caagaagaaa aagaagcttg
       61 tcctgctcga atggcccaag ctcaaggcca tccgcgacca gcttgaccgc gagaccttcg
      121 ggctctacaa gtcggtcaag ggcgtcgtcc gcatcggcca ccagaggctg ctcgacgggg
      181 agaagctcga cgtaatcctg cggatcgcct catggctcga ccccgagagg gacctcgccc
      241 gcgagtggcc gcggaagcgc aatttcgatg cgggggaggg cctccacgag ctcctctcct
      301 cacttgacca cgtgctccag atcgcgaggg ccagggacag gacggccgag ctcggtttcg
      361 tcaccctcta caccgacgcc gagggcatgt tctggttccg ctgctcccgc ggtttccaca
      421 ccgccctgag cgagagcctc gcctcccttg aggcccttgc cgaccgcatc ggcggagacg
      481 gggccgaggg cgcggccgca agccccgagg agagggaaag ggtcaactcc ctctaccgca
      541 agctctcctc attcttcgac tgaaagaggc ccgcatgaga ctgcctggac ttgccatcgc
      601 cttcgccctt ttggcccccc ttcggcttct tgcccagatc gaccccgatc cgggttccgg
      661 tccggacgcg gttccccccg cgctcgggag aggggccgtg aaggatgggc cgctcgttct
      721 tggcctcgag ctcgggatcg gcgccgagtc cgagaccggc ctgccctggg cggtggagcc
      781 cagcctcgcc ctggatgcgg actgggaccg gtactatgcc cgcatcgagc tcagccaggc
      841 cttcggcgag ctggattggc agagggtcct cggctacagg gtcctcgaca cggctggcat
      901 ggccgtggga tggaggcccg agctcgccga tcggctggac ctgcggctcg aggccttcag
      961 ccgctatagg tccttcggcg gagccggcta cgaggccaga ttcggggctc tggccctcct
     1021 tcaggccggc gcgatgcggg gcgaggccgg tcctttcttc atcgcggggg cgggctattc
     1081 aaggctcatg acgaggtact ccggaacggc cgtgagccag tgggacaacg accccctcgc
     1141 ccgcatcggc ttcggatggc gcgacgggcg ccgcctcgtg ctcgacatgg cgatctcgga
     1201 ctacacggga gcggacgcga gcctctggct caagaccttc ttcgagttcg gcggacggct
     1261 tgccttcggc gagggcgagg cgcccgaggc gagggccacg ctggtcctca agtacagcga
     1321 tttcttcacc ctgacctcct acctcgacgg attcgcgttg cgcctctcca tgagtctacc
     1381 ccttggaagc ccggcatggt cggccggcct gacgggaata tgaggaaggc ccacgccgcc
     1441 ctcggcatcg cggcgctcgc cctggtcctc gcctcatgca gctatgggat actcgagggc
     1501 ttcggccgcc ccgaccccgt gggcgagcgg atcgacgagc ccggccagag gtccagcttc
     1561 gccaccgaca agaccctggt cgagggtaac ctcactgcag gcacggcttt caccttcatc
     1621 gtcagctccg acgtccattt cggctacaag ggggggccgc gccaggccgc cctctcggac
     1681 ttcgccgatc tcgccaagac gagcaacgcg gacttcgccc tcttcggcgg ggacgacgcc
     1741 gacaagggca gcgagggcga gtaccaggac ttcagcgcct tcgcggacgg ccttgcgcca
     1801 agcgcggcga cacagctgct cttgggcacg acgaggctcc cctggttctc ggcggtcggc
     1861 aaccacgacc tctacaactc cggctggggc ctcttcagga agtatgtcgg cccctcgtac
     1921 tatgtcctat cggtgggaag cgtcggcatc tatgtgatag acacgggcaa tgggaccctg
     1981 ggcgagaagc agctcgagcg gctcggcgcc gcgatgagcg ccgatccccg acccaagatc
     2041 gtcctgagcc actaccccat ctacggctcg gacaggttca tctactaccg catcacgaat
     2101 ccgagggagc gcatcgagct catcgacctc ttcgccagga ccagggtcgt ggccgtcttc
     2161 gtggggcact ggcactaccc cgaggagagc gatgtagggc ccttcatcga acgggtctcg
     2221 gggagcctcg tggattccga cattgacgga aaggcccacg cctgggtcgt gagcgtcgac
     2281 gccacgggga cccaggtcac cgcgacccgc cacgatttct gaggggccct cggggggccg
     2341 tccgtcctta gctccgctcg aacagcagct cccagaccgc cctgccgtcg cccttcgccc
     2401 tcgcctcgaa cttggtctcg ggcctccagg cctgcttcgg cgcgtagccc tcgtggcggt
     2461 tcctcagtcc gggggtgcgg gagaggagct cgagggacca ctgggcgtac tcctcgatgt
     2521 cggtcacgaa gtagaggtag gccccggggg cgaggcggga ggccagcagc tcggcgaggc
     2581 cctccctgat gagccttcgc ttgtggtgct tcttcttggg ccaggggtcg gggtagaaga
     2641 tgtggagccc cgccagcgag gccggggcga tcatccgctc gagcacctcg acggcgtcgt
     2701 ggtggatgat gcgtatgttt cgcacatcgt gctccgcgat gtcgatgagg agcttcccga
     2761 tgccgggggt gtggacctcg atcccgaggt agcccgtctc cgggttctcg agggcgatcc
     2821 gccaagtcgc ctggcccatg ccaaagccga tctccgcgac caccggccgc cggtcgccga
     2881 acgcggaggt gtagtccagc cgctgccccg gatggaatgg aaggaggtag cgcgggccca
     2941 gctcctcgag ggccctcctc tgggcgtcgg tcatccttcc cgtcctcagc acataggtcc
     3001 gtatgctcgt gtcgccgctc atttcaccac caccctctcc gaataggaac ccatgttccg
     3061 agacggctcg tagccgtagg cctgtatcgc cgcgacccga gccgcgatgt cgaccccgaa
     3121 atgatcgccg aggctctccg ccgccccggt ccacgatgag cttcccagga cggcccccgt
     3181 gccgtcgagc ccgatgatga gggtctcggg ccacgcgctg cgcagggata cctggccctt
     3241 ggagtactcg atcctcgggg cggcatagtc cgggaactgg ccctccaccg agaaggctct
     3301 ttctgccctc tccccagccg ccgtcatcgc catggcgcgg tagccgccgc ggggcagggg
     3361 cccgtagtcg ttcaacgcga ggtcggccgc gccgatccag gtgtccccac cctctgtcct
     3421 cttcgtccag ttcgcgtttg tgagcctcca gtcgatcccg gcggcgtcgt tgacgacgta
     3481 gagggcatcg aggtcgtcga tgccctcgct gtccttgacc gatgcgaaga cccagaggct
     3541 ctcgtagggc cccgccttgg attgtcgctg ctcgagccgc cactcgaccg ccgcgagctc
     3601 gggcggcttg cctccgcagg ccccgatgag ggaggaggcg agtagggcga gcgcggcaag
     3661 ggcgaaggcc cgacctctgt ggagctctgg catcagggcc cgagccgctc ccggaggagt
     3721 ccgatgacgg cggggatcga ggccagttcg acgctttcgc gggtcctgag gtccttgacg
     3781 gcgatggccc cgcgctcggc ctcggccgat ccgagcacaa gggcgaaggg gatgcccttc
     3841 ctctcggcgt aggcgaactg ctgggcgagc ttgcgcttct cggggaagat ctcgcaggcg
     3901 agcccggccg cgcggcagtc cctggccatc gcctggtagc gtggagccag gctctcctca
     3961 aggtcgagga cgaggacctg ggtgtagccg ggcttgcgcc tcgatgcccc gatcgcctcg
     4021 agtgcggcga ggaggcggtc caggcctatc gaggcgccca ccccagggag gcggcggctc
     4081 gtgtagaggc tggcgagctc gtcgtagcgc ccccccgagc agaccgagcc gatcgtgggg
     4141 atggagtcga ggcttgtctc gaacacgacc cccgtgtagt agtccaggcc ccgcgttatc
     4201 gagggcgtga gcgcgaggcg atcggcaagg ccagcgccct cgacgctcgc
//
LOCUS       DASSQV010000070         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_517195, whole genome shotgun sequence.
ACCESSION   DASSQV010000070 DASSQV010000000
VERSION     DASSQV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517195"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFL04_01315"
     CDS             <1..366
                     /locus_tag="VFL04_01315"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET7838366.1"
                     /translation="RARRPRAELDEDIEAAYGSPELEEDPAARLAFSLRETVDELEEP
                     YREALILTEYEGLSQAELAARAGISLSGAKSRVQRAREKLKALLLACCHFELDRRGGI
                     IDYQERCCRCGPAGKGCLP"
     gene            493..1629
                     /locus_tag="VFL04_01320"
     CDS             493..1629
                     /locus_tag="VFL04_01320"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET7838367.1"
                     /translation="MDFAIALLRNSAQQTLVSLSHNWPYLLASILIAAALKVFVDADR
                     ISAFLARHRGAGVLTATAAAVATPFCSCGTTAVVLGMMASTMPLAPIVAFMVASPLSS
                     PEELLYSAGLFGWPFAISYFAASILLGLAGGGIATLLERRGLLAGQVRFEAPAKAEAA
                     PSCGCQAPAPAPAFVRRGAPSFARVETPSFVRLETPALCCEAAAPSPLLAAPLPSGCG
                     CGPSFRESEPEQETSGPSPSSRIGNLLVELGLVAKKLLPMFIGFAFVGYVLNGLIPAS
                     WMSALFGKGHAYSVPLAATVGLPLYVSSEASLPLVRALLDSGMSQGAIMAFLIAGSGT
                     SMGAIAGALTIARWKVVGLVVAVLWLGAILAGYGFDILVASGLA"
     gene            complement(1746..2180)
                     /locus_tag="VFL04_01325"
     CDS             complement(1746..2180)
                     /locus_tag="VFL04_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838368.1"
                     /translation="MRYQLSRARTLSLLLGLAALLGLGSLRLAAQAAVTPVVVVQDQA
                     IDDSRVIVLKVTSATPGWMVIHADAAGRPGPVIGYAAVREGENLNVVVAIDAKKATPV
                     LYAMLHVDAGQIGSYEFPGPDVPTMNMGAMVSPAFKASPQAY"
     gene            2316..3188
                     /locus_tag="VFL04_01330"
     CDS             2316..3188
                     /locus_tag="VFL04_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838369.1"
                     /translation="MQSYICARCGAQFAETEGPPERCPVCDEPRQYLGPKGQEWTTMA
                     ELARTSRNIFRPIEAGLTGISTEPAFAIGQRAMLISTPAGNVLWDCLSYIDRATVQEI
                     RSRGSLAAIAVSHPHYYASVVEWSRSFGGVPVYLHADDAPWLMRSDPCVVHWEGERLE
                     IAPGATLVRVGGHFPGSTVLHWRDGVAGRGSLHTGDSIYVVADKNWVSFMYSYPNLIP
                     LAPEAVRRIVASLEGFEFERIYGAWWDSVVQADAKRVLAASAERYIAAIGGRPPSADG
                     RFIGSGSLPRAALA"
BASE COUNT          457 a         1238 c         1116 g          545 t
ORIGIN      
        1 cgcgcgcggc gccctcgggc cgagctcgac gaggacatcg aggcagccta cggctcgccc
       61 gagctagagg aggacccggc ggccagattg gccttctccc tcagagagac cgtggacgag
      121 ctcgaggagc cctatcggga ggccctcatc ctcacggaat acgagggcct gagccaggcc
      181 gagctggcgg cgcgggcggg catctcgctc tcgggcgcga aatcccgggt ccagcgcgcc
      241 cgggagaagc tcaaggccct tctcctcgcc tgctgccact tcgagctcga ccggaggggc
      301 gggatcatcg attaccagga gcgctgctgc cgctgcggcc cggcggggaa gggctgcctc
      361 ccctaggacc gcggccagcc attggcgagc cgccccgggc gagtttccgg ggccgcccct
      421 catttttcct gcgtccttct gcccgggcct ccgtctgtcc tgccgaacag cagttcacag
      481 ggaggaacac agatggattt cgcaatcgcc cttctgagga attcggccca acagaccctg
      541 gtcagccttt cgcacaactg gccctacctg ctcgccagca tcctcatcgc agcggccctc
      601 aaggtcttcg tggacgccga ccgcatctcg gctttcctcg cgcgccaccg cggggctggg
      661 gtcctcaccg cgaccgccgc cgcagtcgcc acccccttct gctcctgcgg cacgacggcc
      721 gtcgtcctcg gcatgatggc cagcaccatg ccgcttgcgc ccatcgtggc cttcatggtc
      781 gcctcgccgc tgagctcgcc cgaggagctc ctctacagcg ccggcctctt cggctggccc
      841 ttcgccatct cctattttgc ggcctccata ctgctcggcc tcgccggggg cggcatcgcc
      901 accctcctcg agaggcgcgg cctcctcgcc ggccaggtcc gcttcgaggc ccccgccaaa
      961 gccgaggcgg ccccgtcctg cggctgccag gcgcctgccc cagcccccgc cttcgtccgc
     1021 cgcggggcgc cgtcttttgc gcgggtcgag acgccgtcct tcgtgcggct cgagacgccg
     1081 gccctctgct gcgaggccgc ggccccctct cccctcctcg ccgctccgct cccctcgggc
     1141 tgcggctgtg ggccctcgtt ccgggagagc gagcccgagc aagagacctc agggccgtcc
     1201 ccatcgtccc ggatcgggaa cctcctcgtc gagctcggcc tggtcgcgaa gaagctcctg
     1261 cccatgttca tcggcttcgc cttcgtgggc tacgtcctca acggcctcat ccccgcttcc
     1321 tggatgtcgg ccctcttcgg caagggccac gcctacagcg tccccctcgc cgcgaccgtc
     1381 ggcctgcccc tctacgtcag ctccgaggcc tccctgcccc tcgtccgcgc cctgcttgac
     1441 tccggcatga gccagggggc gatcatggcc ttcctgatcg ccggctcggg cacctcgatg
     1501 ggcgccatag cgggggccct gacgatcgcg cggtggaagg ttgtgggact cgtcgtcgcg
     1561 gtgctctggc ttggggccat cctggccggc tacggcttcg acatcctggt cgcctcgggc
     1621 ctggcctagg cccggttcag ctcagcccca ggctccaccc caaccgcccg gccaaagccg
     1681 ggcggttttg ctgtacgcgg gaccttggcc ccaagcgttt cagtaggctt ggggcctcaa
     1741 gcgcatcagt aggcttgggg gctggccttg aaggccgggc tcaccatggc gcccatgttc
     1801 atcgtgggga cgtcggggcc ggggaactcg tagctgccga tctggccggc gtcgacgtgg
     1861 agcatcgcgt agagcaccgg ggtggccttc ttggcgtcga tcgccaccac gacgttcagg
     1921 ttctcgccct cgcgcaccgc ggcatagccg atgacggggc cgggcctccc cgcggcgtcg
     1981 gcgtggatga ccatccatcc gggggtggcg ctcgtgacct tgagcacgat cacgcgcgaa
     2041 tcgtcgatgg cctggtcctg gacgacgacc acgggcgtca ccgccgcctg ggcggcgaga
     2101 cggagagagc caaggcccag cagggccgcg agtccgagaa gcagcgacag ggtcctcgcg
     2161 cgggatagct gataacgcat gcacatcctc cttccctgaa tcgttcaggg tgcgcactgt
     2221 ctacgggcgc cgcagggctt cggatcgacc cgccgccgga atatcgaggg ccccaggcct
     2281 tggtcccgga cggcggggct cggtatcttc ccctcatgca gagctacata tgcgcgcgct
     2341 gcggagcgca gttcgccgag accgagggcc cgcccgagcg ttgcccggta tgcgatgagc
     2401 cccggcaata ccttgggcca aagggccagg aatggacgac gatggccgag ctcgcgagga
     2461 cgagcaggaa catcttccgc cccatcgagg ccgggctgac gggcatctcc accgagccgg
     2521 ccttcgccat cggccagagg gcgatgctca tctcgacccc ggcgggcaac gtcctctggg
     2581 actgtctgag ctacatcgac agggcgacgg tccaggagat caggtcgagg ggctcgctcg
     2641 ccgcgatcgc ggtctcgcat ccccactact acgcctcggt cgtcgagtgg tcgaggagct
     2701 tcggcggggt cccggtctac ctccacgccg acgacgcccc atggctcatg cggagcgatc
     2761 cctgcgtcgt ccactgggag ggcgagcggc tcgagatcgc gccgggggcg accctggtcc
     2821 gcgtcggcgg ccactttccc ggatccacag tcctccactg gagggacggc gtcgcgggca
     2881 ggggcagcct ccacaccggc gactcgatct acgtcgtggc ggacaaaaac tgggtgagct
     2941 tcatgtacag ctaccccaac ctcatccccc tcgcgcccga ggcggtgcgc cggatcgtgg
     3001 cctcgctcga aggcttcgag ttcgagcgca tctacggcgc ctggtgggat tccgtggtcc
     3061 aggccgacgc gaagcgggtc ctggcggcct cggccgagcg ctacatcgct gcgatcgggg
     3121 gccggccccc gtccgcggac ggccggttca tcgggagcgg gtcgctaccc cgggcggcgc
     3181 tcgcataagg ccctaggtga tgtcggcgaa cacggccccg gccagggccg cgagggcccc
     3241 gggctcgagg atgacctgga ccccccgcct tcccgccgac accgagacct cgtcaaagag
     3301 ggtggcggtc tcgtcgatca gggtggcgaa cttccgcttc gtgccgaggg gggagc
//
LOCUS       DASSQV010000071         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2383175, whole genome shotgun sequence.
ACCESSION   DASSQV010000071 DASSQV010000000
VERSION     DASSQV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2383175"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="VFL04_01335"
     CDS             <1..1550
                     /locus_tag="VFL04_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7838370.1"
                     /translation="PGMELLSARGLSKYFPETATLANDAVSLGLAEGEIKAVVGENGA
                     GKSTFARILAGFLAPDSGELWVGGARLRPGSVREAEAAGIGYVPQQSLLADGLTVAEN
                     LSLGGEQRRLGFLYDRRRAVVEAAALVERYGFHLDPAARASELSPPERRQAEIARALA
                     RGGRILVLDEPTSILAESEAEALFALLRRLADSGKGVIFITHRVGEVLGFADSIAVLR
                     EGRVVAELAAGAVGEAGLSRLMSRSGAAGLTTGAGRRSPSTLESCAEAPRRVALELSG
                     LVLARGSRPLSLSVRAGEVLGVAALVGNGLGRLEDYLSGLKPAPWGAVRLGGTDLHDI
                     PRHRLRTELLAYVPSDREARGIAETASLGDNVLVLRRREFGIVDFAMKARLRESARSM
                     AASFSLSAEPSVAAGSLSGGNRQRLVLARELDRPRRLAVLAEPFQNLDLGSQEAAAGR
                     IAELAAAGSAVVVLSSSLDELFPIADRIAILYRGSLVHLGPNGGPGSAHALLALMTGS
                     APARTPV"
     gene            1547..>2091
                     /locus_tag="VFL04_01340"
     CDS             1547..>2091
                     /locus_tag="VFL04_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838371.1"
                     /translation="MRRPDRRRAWLARLAAFLLALGLAAVYAFLASREPMRALSSFFL
                     GPFSSRYALFALLEGAGPLLVCALGAAISFRSGSFNLGGEGQAAVGCLASALVMRSLG
                     PGSSAPPELVLPLCLLAAAAGGALLASLSALAERWSGAEILLTTFLLSQAALILVDWA
                     IGGPFRDLGSNLLAMPSVPGR"
BASE COUNT          229 a          825 c          713 g          324 t
ORIGIN      
        1 ctcccggcat ggagctgctc agcgcccgcg gcctcagcaa atacttcccc gagaccgcga
       61 ccctcgccaa cgacgccgtc tccctcggcc tggccgaggg ggagatcaag gccgtcgtcg
      121 gggagaacgg ggcgggcaag agcaccttcg ccagaatcct cgccggcttc ctcgcccccg
      181 actcgggcga gctctgggtc ggcggcgcga ggctcaggcc gggcagcgtc cgcgaggccg
      241 aggcggcggg gataggctac gtgccccagc agtccctcct cgccgacggc ctcacggtgg
      301 ccgagaacct ctcgctcggc ggcgagcagc ggaggctcgg cttcctctac gacaggaggc
      361 gggccgtggt cgaggcggcc gccctcgtcg agcgctacgg tttccacctc gatccggccg
      421 ccagggcctc ggagctctcg cccccggaga ggcgccaggc cgagatcgcc agggccctgg
      481 cccggggcgg caggatcctg gtcctcgacg agcccacctc gatcctcgcc gagagcgagg
      541 ccgaggccct gttcgccctc ctgcgccgcc tcgccgactc gggcaagggg gtgatcttca
      601 tcacccaccg ggtcggcgag gtcctgggct tcgccgacag catcgccgtc ctgagggagg
      661 gcagggtggt ggccgagctc gccgccgggg ccgtgggcga ggccggcctc tcgcggctga
      721 tgtcccggag cggggcggcc gggctcacga cgggagccgg gcggcggagc ccctcgaccc
      781 tggagtcctg cgccgaggcg ccaagacggg tcgccctcga gctgtcgggc ctcgtccttg
      841 cccggggctc ccggcccctg tccctctcgg tccgcgcggg tgaggtcctc ggcgtcgccg
      901 cccttgtggg caacggcctt ggccggctcg aggactacct ctcggggcta aagccggcgc
      961 cttggggcgc cgtccgcctc ggggggaccg acctgcacga catcccccgg caccggctca
     1021 ggaccgagct cctcgcctac gtgccctcgg accgcgaggc cagggggata gcggaaacgg
     1081 cgagcctcgg tgacaatgtc ctcgtgctga ggcgaaggga gttcgggatc gtcgacttcg
     1141 cgatgaaggc caggctcagg gaaagcgcgc ggtcgatggc cgcatccttc tcgctctccg
     1201 ccgagccctc tgtggcggct ggctccctgt ccggcggcaa ccgtcagcgc ctcgtcctgg
     1261 cccgtgagct cgaccgcccg cgccgcctcg ccgtcctcgc cgagcccttc cagaacctcg
     1321 acctcggttc ccaggaggcg gcggcggggc ggatcgccga gctcgcggcg gccggaagcg
     1381 ccgtcgtcgt cctctcctcg agcctcgatg agctcttccc catcgccgac cgcatagcga
     1441 tcctctaccg cggctccctc gtccaccttg gccccaacgg gggacccggg agcgcccacg
     1501 ccctcctcgc gctgatgaca ggttcggcgc ccgcgaggac ccccgtttga ggaggccgga
     1561 ccggaggagg gcctggctcg cgcggctcgc cgccttcctc ctcgccctcg gcctcgccgc
     1621 cgtctatgcc ttcctcgcga gcagggagcc catgcgggcc ctctcctcct tctttctcgg
     1681 gcccttctcc tcgcgctatg ccctcttcgc cctcctcgag ggcgccgggc ccctcctggt
     1741 ctgcgccctc ggggcggcga tctccttccg gtcaggatcc ttcaacctcg gaggggaggg
     1801 ccaggcggcg gtcggctgtc tggcctcggc cctcgtcatg cggtccctcg gacccggctc
     1861 ctctgcccct cccgagctcg tcctgcccct ctgtctcctc gcggccgccg cgggcggggc
     1921 cctcctcgcc tccctctcgg ccctggccga gcgttggtcg ggagccgaga tcctcctcac
     1981 gaccttcctc ctgtcccagg ctgccctgat cctcgtggac tgggccatcg gcggcccctt
     2041 ccgggacctc ggcagcaatc tcctcgccat gccgagcgtg cccgggcgct a
//
LOCUS       DASSQV010000072         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_269008, whole genome shotgun sequence.
ACCESSION   DASSQV010000072 DASSQV010000000
VERSION     DASSQV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269008"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1644
                     /gene="secD"
                     /locus_tag="VFL04_01345"
     CDS             <1..1644
                     /gene="secD"
                     /locus_tag="VFL04_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET7838372.1"
                     /translation="TPKEDQALAVGSREQIREYARRMAYTDLTELKKAAGANDESPVP
                     SAKYPAVFSTAQKAYKDAKKALPKAWTAKSLLAAYDNERAAFDAVENVYRTRIMSLKA
                     KKGKAIQLGLDLSGGMSVVIQADMDSLAKKLGHSLSAAEKNDAMKRGIEVLNSRIDKF
                     GLTEPVIRPQGEDQIYVEIPGAADPERINSIIMGRGKLAFHIVNDEASAKLDQLLATN
                     PLAVDEKTMTVSDPNLIPAGNVIRKFYKKDSYGLDEFTGTYKVIGEVPGLDGTHIQSA
                     QVSSDPITGAPEINFVLDKEGGELFYKLTSANVGKTMSVVLDDRIKAGARIQEPIRDQ
                     VRMTGFGADEAQNIALLLRTAALPIELSPVNQQAIGASLGEDAVRSGILAVTIAFFLV
                     IGFMLVYYKGAGVNTAIAEVVNLFIMIGVLSAFGLTLTLPSIAGFVLTIGVSVDANVL
                     IFERIKEELRLGKGRVSSIKAGFNKAFWTIFDSHLTNLIAALFLAQLGSGPIQGFAVS
                     LAIGTVCSLFTSLFVSHLIFDFETDVMKIKHTSISWRVK"
     gene            1641..>2296
                     /locus_tag="VFL04_01350"
     CDS             1641..>2296
                     /locus_tag="VFL04_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838373.1"
                     /translation="MTRVINFDRSFKITVPISVVIIIAGLFGAAFIGFNNGVDFQAGL
                     NTSIQFAPPSMRVSFAGEGTMRLSVTKADVELISQAPSGQSKAYLFAYDKYPTIQAMA
                     DALKTAPGVQVELVAKGDLASNQILGASQTDSALSATPTVLHVRSQDPNALMIPVESL
                     RGTMKGFGNVSIQRAGDAANREFVIRVEDSGKDPTFATTIHQRLVQTLGQAYGADNVL
                     V"
BASE COUNT          426 a          871 c          649 g          350 t
ORIGIN      
        1 acccccaagg aggaccaggc cctcgccgtg ggaagccgcg agcagatcag ggagtacgcc
       61 cgccgcatgg cctacaccga cctgaccgag ctcaagaagg cggcgggcgc caacgacgag
      121 agccccgtcc cctcggcgaa gtaccccgcg gtcttctcga ccgcccagaa ggcctataag
      181 gacgccaaga aggccctccc caaggcctgg accgccaaga gcctcctcgc cgcctacgac
      241 aacgagcgcg ccgccttcga cgcggtcgag aacgtgtacc gcacccggat catgagcctc
      301 aaggccaaga agggcaaggc gatccagctc ggcctcgacc tctcgggcgg catgagcgtc
      361 gtgatccagg ccgacatgga ttcgctggcg aagaagctcg gccacagcct ctccgcggcc
      421 gagaagaacg acgccatgaa gcgcggcatc gaggtcctca acagccgcat cgacaagttc
      481 ggcctcaccg agcccgtcat caggccccag ggcgaggacc agatctacgt cgagatcccc
      541 ggcgccgccg accccgagcg catcaactcg atcatcatgg gccgcggcaa gctcgcgttc
      601 cacatcgtga acgacgaggc ctcggccaag ctcgaccagc tcctcgcgac caaccccctg
      661 gcggtcgacg agaagaccat gaccgtgtcc gaccccaacc tcatccctgc cggcaacgtc
      721 atccgcaagt tctacaagaa ggacagctac ggcctcgacg agttcaccgg cacctacaag
      781 gtgatcggcg aggtccccgg tctcgacggc acccacatcc agagcgccca ggtctcctcg
      841 gacccgatca ccggcgcgcc cgagatcaac ttcgtcctgg acaaggaggg cggggagctc
      901 ttctacaagc tcacgagcgc caacgtcggc aagaccatgt cggtggtgct ggacgaccgc
      961 atcaaggccg gcgcccgcat ccaggagccc atccgcgacc aggtccgcat gaccggcttc
     1021 ggcgccgacg aggcgcagaa catcgccctc ctcctgcgca ccgcggccct gcccatcgag
     1081 ctcagccccg tcaaccagca ggccatcggc gcctcgctcg gcgaggacgc cgtccgctcg
     1141 ggcatcctgg cggtgaccat cgccttcttc ctcgtcatcg gcttcatgct cgtctactac
     1201 aagggcgcgg gcgtcaacac cgccatcgcc gaggtggtca acctcttcat catgatcggc
     1261 gtcctgtcgg ccttcggcct gaccctcacc ctgccctcga tcgccggctt cgtgctcacg
     1321 atcggcgtct cggtagacgc caacgtgctg atcttcgagc gtatcaagga agagctcagg
     1381 ctcggcaagg gccgcgtgtc gtccatcaag gcgggcttca acaaggcctt ctggacgatc
     1441 ttcgactccc acctcacgaa tctcatcgcc gccctcttcc tggcccagct cggctccggc
     1501 cccatccagg gcttcgccgt gtcgctcgcc atcggcaccg tgtgctcgct gttcacctcg
     1561 ctcttcgtct cgcacctgat cttcgacttc gagaccgacg tcatgaagat caagcacacg
     1621 agcatctcct ggagggtcaa atgactcgcg tcataaactt cgaccgcagc ttcaagatca
     1681 ccgtcccgat ctccgtggtg atcatcatcg cggggctctt cggcgcggcc ttcatcggct
     1741 tcaacaacgg cgtcgacttc caggccggcc tcaacacctc gatccagttc gcccccccct
     1801 cgatgcgcgt ctcgttcgcc ggcgagggga cgatgcgcct gtcggtgacc aaggccgacg
     1861 tcgagctcat ctcccaggcc ccctcgggcc agagcaaggc ctacctcttc gcctacgaca
     1921 agtacccgac catccaggcc atggccgacg cgctcaagac cgcccccggg gtccaggtcg
     1981 agctggtcgc caagggcgac ctcgcctcga accagatcct cggcgcctcc cagaccgact
     2041 cggccctctc ggcgaccccg acggtcctcc acgtgcgcag ccaggacccc aacgccctca
     2101 tgatcccggt ggagtccctc cgcggcacga tgaagggttt cggcaacgtc tcgatccaga
     2161 gggcgggcga cgccgccaac cgcgagttcg tgatccgcgt cgaggactcg ggcaaggacc
     2221 cgaccttcgc gaccacgatc caccagaggc tcgtgcagac cctcggtcag gcctacgggg
     2281 ccgacaacgt cctcgt
//
LOCUS       DASSQV010000073         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1263920, whole genome shotgun sequence.
ACCESSION   DASSQV010000073 DASSQV010000000
VERSION     DASSQV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263920"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFL04_01355"
     CDS             <1..230
                     /locus_tag="VFL04_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7838374.1"
                     /translation="DNPSGVIVTGVVEGSPADTAHIDVNDVILGIGNAEVTDSVQFNS
                     RIAEALKSPSVIFLLRDNSTGRTGFLNVKLR"
     gene            complement(237..1127)
                     /gene="thrB"
                     /locus_tag="VFL04_01360"
     CDS             complement(237..1127)
                     /gene="thrB"
                     /locus_tag="VFL04_01360"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET7838375.1"
                     /translation="MRRARVRVPATSANIGPGFDCLGLGLDLYNFYEIREAEGLVISG
                     CAPEHSGEDNLFLRAFRRGMEELGLSFKGARVAIAADIPVARGLGSSSACIVGGLMAA
                     SAFGAREMGQERMLGLATEMEGHPDNVAAALLGGFVVSVADGQGVATVRALVSPELTF
                     CALVPPFALETVKARAALPRTVEFADAAYNAGRSALVTAAFLGGDYGKLATACGDRLH
                     QPYRSPLIPGYDEVLAAARDAGAVSVYLSGAGPTIMAICDRRRERSFAEAIGPVLGSR
                     AEGAWRLMVLHADNEGARLM"
     gene            complement(1124..>2333)
                     /gene="thrC"
                     /locus_tag="VFL04_01365"
     CDS             complement(1124..>2333)
                     /gene="thrC"
                     /locus_tag="VFL04_01365"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET7838376.1"
                     /translation="LELFHGSTLAFKDLALSLMGGLVGMARERLGESRDLLVLVATSG
                     DTGKAALAGFSDRPGLRVAVFYPERGVSPVQRLQMVSHAAGNAFVAGIHGDFDQAQGG
                     VKEIFSSAQTASELGNRGFALTSANSINVARLLPQIVYYVKAWRDLRDAGGLGSGGTM
                     DVAVPTGNFGNILAAWYAKRMGLPIGRLICASNRNHVLADFFATGRYDRNRSLHQTAS
                     PSMDILVSSNLERLVFAASGEDPHRTAELMLSLSRSGSFSLRGSERAFLGEFVADWAD
                     DEEAGSEIARVFSESGYLIDPHSAVAAAVLGKIRRREGETRPTVLASTASPFKFPGFV
                     ARAVGLGPASATGTEAELDLADRLAEHAGLELPAAVSRLRGASVLHGSVVEPDQMKAA
                     LLGFIDGGRA"
BASE COUNT          376 a          815 c          802 g          340 t
ORIGIN      
        1 tcgacaaccc ctccggggtc atcgtcaccg gggtggtgga aggcagtccc gcggacactg
       61 cccacatcga cgtgaatgac gtcatcctcg gcataggcaa tgccgaggtg acggactcgg
      121 tccagttcaa ctccaggatc gccgaggcgc tcaagtcccc ttcggtgatc ttcctgctca
      181 gggacaacag caccggcagg acgggcttcc tgaacgtcaa gctgcgctga aggccatcac
      241 atcagccgcg cgccctcgtt gtcggcgtgg aggaccataa gccgccaggc cccctcggcc
      301 cgagacccga gaacgggacc gatcgcctcc gcgaagctcc gttcgcgcct gcggtcgcag
      361 atcgccatga tggtgggccc cgccccgctc aggtagaccg agaccgcccc cgcgtcgcgg
      421 gccgcggcga ggacctcgtc gtacccgggt atgaggggag agcggtaggg ttggtggagc
      481 cggtcaccgc aggccgtggc gagcttgccg tagtccccgc cgaggaaggc ggctgtcacc
      541 agggcggatc ggcccgcgtt gtacgcggcg tcggcgaact cgaccgtccg cggcagggcc
      601 gccctcgcct tgaccgtctc gagggcgaag ggagggacga gggcgcagaa ggtgagctcg
      661 ggcgagacca gggctcggac cgtcgccacg ccctgcccat cggcgaccga gaccacgaat
      721 ccgccgagca gggccgccgc gacattgtcc gggtgtccct ccatttcggt cgccagcccg
      781 agcatcctct cctggcccat ctcgcgggcc ccgaaggccg aggcagccat gaggcctccg
      841 acgatgcagg ccgagctcga cccgaggccc cgggcgacgg ggatgtcggc cgcgatggcc
      901 acccgagctc ccttgaagga caagcccagc tcctccatgc ccctgcggaa ggcgcgcagg
      961 aagaggttgt cctcgcccga gtgttcgggg gcgcagcccg agatgacaag gccctcggcc
     1021 tccctgattt catagaaatt gtaaaggtcg aggccgaggc caagacagtc aaagcccggc
     1081 ccgatgttcg cgctcgtggc cggcacccgc acccttgccc gcctcaagcc cgtcccccgt
     1141 ctatgaagcc gagaagggcc gccttcatct ggtccggctc gacgaccgag ccgtgcagca
     1201 cggaggcgcc ccggagccgg gagacggcag cgggcagctc caggcccgcg tgctcggcga
     1261 gcctgtcggc gaggtcgagc tcggcctcgg tcccggtcgc cgatgctggg ccaaggccga
     1321 cggcccttgc cacgaagccg gggaacttga agggactggc ggtcgacgcg aggacggtgg
     1381 gcctcgtctc gccttcccgt ctcctgatct ttccgaggac ggcggccgcg accgcgctgt
     1441 gcgggtcgat caggtagccg gattcggaga agaccctcgc gatctcggac cccgcctcct
     1501 cgtcgtctgc ccagtcggcg acgaactcgc cgaggaaggc ccgctcggag ccccgcaggg
     1561 agaaggaccc agaccgcgag agggagagca tgagctcggc cgtcctgtgg ggatcctcgc
     1621 cgctggccgc gaagaccagg cgctcgaggt tgctcgagac gaggatgtcc atcgaggggc
     1681 tcgctgtctg gtggaggctc cggttcctgt cgtagcgtcc ggtggcgaag aagtcggcga
     1741 ggacgtggtt gcggttcgag gcgcagatga gcctgccgat cggcagcccc atgcgcttcg
     1801 cgtaccaggc cgcgaggatg ttcccgaagt tgcccgtcgg cacggccacg tccatggtcc
     1861 cgccgctgcc gagtcctccc gcatcgcgca ggtcccgcca ggccttcacg tagtagacta
     1921 tctgcggcag gaggcgcgcc acattgatcg agttggccga ggtcagggcg aaaccgcggt
     1981 tcccgagctc gctcgccgtc tgggcgctgg agaagatctc cttcacgccc ccttgggcct
     2041 ggtcgaagtc cccgtggatg ccggccacga atgcgttgcc cgcagcatgg ctcaccatct
     2101 gaagccgctg tacggggctc acgcccctct ccggatagaa gaccgccacg cgcagaccgg
     2161 gcctgtccga gaacccggcg agggcagcct tgcccgtgtc tcccgaggtg gcgacaagga
     2221 cgagcaagtc ccggctctct ccgagcctct ccctggccat gcccaccagg ccgcccatga
     2281 ggctcagggc caggtccttg aaggcgaggg tcgacccgtg gaagagctcc agg
//
LOCUS       DASSQV010000074         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_394063, whole genome shotgun sequence.
ACCESSION   DASSQV010000074 DASSQV010000000
VERSION     DASSQV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394063"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFL04_01370"
     CDS             <1..203
                     /locus_tag="VFL04_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HET7838377.1"
                     /translation="IRDAVDFRTFFAEAPAMNPKCALIKGLVCGVRVEEVEDPLMRNI
                     RYLDKLVDELAKGRPMDKILRQ"
     gene            211..720
                     /locus_tag="VFL04_01375"
     CDS             211..720
                     /locus_tag="VFL04_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7838378.1"
                     /translation="MMQKSGAFGSRLAGAGDAEAIHALYEAAKEWGRQGGSSDWDESY
                     PNREILMEDLEQGRIYVHEEGARIVAAISLLKDWRIDIAPHAWEEEDACSLARLCVHP
                     ELQGRGLGESMMRMISERAKAMGFKATHHLASKSNPAANRLYERMGYLRKGELALYDT
                     DFIAYELPL"
     gene            737..1255
                     /locus_tag="VFL04_01380"
     CDS             737..1255
                     /locus_tag="VFL04_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838379.1"
                     /translation="MGNDLLARGIASQLLAGLAMLKDCIDRCPEKDWHEGHGDYPFSR
                     VAFHALFDCDYHLSEGERELKAQPFHQANRAAFGDYEELEDRGTKGMYDRGFVQSYFE
                     HCRGKVEAAIEGRTAAELLVPNSDVRGNMTKLERYVNTIRHLQHHAAQLGLRLQFLTG
                     KEMDWVARGHGQ"
     gene            1261..2172
                     /locus_tag="VFL04_01385"
     CDS             1261..2172
                     /locus_tag="VFL04_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7838380.1"
                     /translation="MVLSVASFNAENFYLLIEPGIGRAELEALDGPDYMAMNASIFNP
                     NKERGKIAEIARTILEGGFDLVGLCEVGGMESLEAFNRIYLEGRYVCFLHEENSRRGI
                     FVGALLRKGRFPEARAHNMAGAFSRNLLRLDLGPAGGGLVVFVVHLKSQYGPDRGLGP
                     RIREVERLRSLVRTVNCVVMGDFNGILIRGEHQFEYEPFLELPFTDVLEAVGVPPGER
                     RTHYHFGPAPNFTQLDYIFCTDDIEVLDAGVIEGDIPLSRAERARLPSDHLFIRARIR
                     LSEGAGALGASGLWARLRRWWALRRRR"
BASE COUNT          421 a          744 c          775 g          379 t
ORIGIN      
        1 agattaggga cgcagtcgat ttccgcacct tcttcgccga ggccccggcg atgaacccca
       61 aatgcgccct catcaagggc ctggtctgcg gggtccgcgt cgaggaggtg gaggatcccc
      121 tcatgcggaa catccgctac ctcgacaagc tcgtcgacga gctcgcgaag ggccggccga
      181 tggacaagat cctgaggcag tagccgcccg atgatgcaga aaagcggggc cttcgggtcc
      241 aggctggcag gcgcgggaga cgccgaggcg atccacgccc tgtacgaggc cgcgaaggaa
      301 tgggggaggc agggggggag ctccgactgg gacgagagct atccgaaccg ggagatcctc
      361 atggaggatc tggagcaggg ccgcatctat gtccacgagg agggcgcacg gatcgtggcg
      421 gcgatctccc tccttaagga ctggaggatc gacatcgccc cccacgcctg ggaggaggag
      481 gacgcctgct cgctcgctag gctctgcgtc caccccgagc tccagggcag gggcctgggc
      541 gagtcgatga tgaggatgat cagcgagcgt gcgaaggcga tgggcttcaa ggcgacccac
      601 cacctggcct cgaagtcgaa cccggcggcg aaccggctct acgagcggat gggctacctc
      661 aggaaggggg agctcgcgct ctatgacacg gacttcatcg cctatgaact gccgctctag
      721 gccaggaggc tcggatatgg gaaacgactt gctcgccagg ggaatcgcca gccagctcct
      781 ggccggcctc gcgatgctca aggactgcat cgacagatgc cccgagaagg actggcacga
      841 gggccatggg gactacccct tctcccgggt ggccttccac gcccttttcg actgcgacta
      901 ccatctcagc gagggcgaga gggagctgaa ggcccagccc tttcaccagg cgaacagagc
      961 ggccttcggc gactacgagg agcttgagga caggggcacg aagggcatgt acgaccgcgg
     1021 tttcgtgcag agctatttcg agcactgcag gggcaaggtc gaggcggcca tagagggcag
     1081 gacggcggcc gagctcctcg tccccaactc cgacgtccgc gggaacatga cgaagctcga
     1141 gcgctatgtg aacaccatac ggcacctcca gcaccacgcc gcccagcttg ggctcaggct
     1201 ccagttcctc acggggaagg agatggactg ggtcgccagg ggtcatgggc agtgagttgc
     1261 atggtcctga gcgtcgcctc cttcaacgcc gagaacttct acctcctcat cgagcccggg
     1321 attggacgcg cggagctcga ggccctcgac ggccccgact acatggccat gaacgcctcg
     1381 atcttcaacc ccaacaagga gaggggcaag atcgccgaga tcgcccggac catcctcgag
     1441 ggcggcttcg acctcgtggg gctctgcgag gtcggcggca tggagagcct cgaggccttc
     1501 aaccgcatct acctcgaggg ccgctacgtc tgcttcctcc acgaggagaa ctcgaggcgg
     1561 ggcatcttcg tcggggctct gctcaggaaa ggccgcttcc ccgaggcgag ggcccacaac
     1621 atggccgggg ccttctcgcg gaacctgctc aggctcgacc tggggccagc cggtggggga
     1681 ctcgtggtat ttgtggtcca cctgaagtcg cagtacggcc ccgaccgggg cctcgggcca
     1741 aggatccgcg aggtggagag gctgcgttcc ctcgtgcgga cggtgaactg cgtcgtgatg
     1801 ggcgacttca acggcatcct catccgcgga gagcaccagt tcgagtacga gcccttcctc
     1861 gagctgccct tcaccgacgt cctcgaggcc gtgggcgtgc cgcccgggga gcgcaggacc
     1921 cactaccatt tcgggccggc gcccaacttc acccagctcg actacatctt ctgcaccgac
     1981 gacatcgagg tccttgacgc cggcgtgatc gagggcgaca ttccgctgag ccgcgccgag
     2041 cgcgcccgcc tgccctctga ccacctcttc ataagggcga ggatcaggct gtccgagggg
     2101 gctggggcgc tcggtgcgtc ggggctctgg gccaggctcc gccgctggtg ggctcttcgt
     2161 cgccgacgtt gactttcgcg ttgtacgccc gcaaattaga ctgcaggaga ccaaagatga
     2221 tagatttcgc cgtatcggtc ctgcccctat tcgtggtcgc gatcgcgaac ttcgtcctga
     2281 gctggctcta ctattccccc gccgtgccct ggttcaagg
//
LOCUS       DASSQV010000075         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1387348, whole genome shotgun sequence.
ACCESSION   DASSQV010000075 DASSQV010000000
VERSION     DASSQV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387348"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="VFL04_01390"
     CDS             <1..1599
                     /locus_tag="VFL04_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HET7838381.1"
                     /translation="FYVEVKPEDAKEIIAEQVVKGREVKRLLYRKSAKSSDTVKVEDI
                     EFYQKQFRIVLRNCGVINPENIDEYIAREGYTGLERALFTMKPEDVIQELKTSGLRGR
                     GGAGFPTWLKWDLTIKAKGEVKYVVCNADEGDPGAYMNRSTIEGDPHSILEAMTICGY
                     TIGARKGYIYIRAEYPLAIERLKIAIKQAREYGLLGEDILGSGFSFDLEIRLGAGAFV
                     CGEETALLQSLEGKRGMPVPKPPFPAVSGLWKKPTVINNVETYATVAFIMAKGAEWFA
                     KIGTEKSKGTKTFALTGKINNSGLVEVPMGTTLREIVYDIGGGIRGGKAFKGVQTGGP
                     SGGIITAESLDQPITYESLSALGSMMGSGGMIIMDEDDCVVDISKFYMAFCVDESCGK
                     CAPCRIGTSQMHAILDRISKGAGTEEDLAKLEKIGKAMTKASLCLLGGSAANPTISTL
                     KHFREEYLEHIRDHRCRAGKCKDLVVYEIDAAKCIGCGLCARKCPVPCIAGDKKQAHV
                     IDKARCVKCGECFKACKFGAVSKK"
     gene            1621..3372
                     /locus_tag="VFL04_01395"
     CDS             1621..3372
                     /locus_tag="VFL04_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HET7838382.1"
                     /translation="MINAKINGIPVQVAEGTTILDAARKVEVKIPTLCYNDDLEPWAA
                     CGICVVKAQGSAKMLRACCTPIDEGMSIITHDPDIVQTRKTVIELILSTHPDDCLACP
                     RNQECELQRLAAEFGIREQPFPKAVRELPLDDSTGSLILNPEKCIRCGRCVKVCQDMQ
                     NVWAIEFLGRGEKTRIAPAADVKLGEGPCIKCGQCSAHCPVGAIYENDQTALVWDALM
                     KEGAEAKTCVVQIAPAVRVALGEAFGLEPGTNLTRKIYTALRRIGFDVVFDTNFAADL
                     TIIEEATEFVKRFTGAGPLPLITSCCPAWVDYLEKYYGDMIPNFSTAKSPQQMMGAMI
                     KTYWAAKAGLPSEKVFSVSVMPCTAKKFETHRDPTMYSSGHQDVDVSITTRELARMIK
                     QAGIDLVNLPESEPDSPMGPHSGAGVIFGATGGVMEAALRTAYNYVTGEDLKDVNFSG
                     VRGISGIKEASVHIKGTEVRVAVAHQMGNIAQVLDMVRKAKAEGRETPWHFIEVMACR
                     GGCIGGGGQPYGCTDEVRKLRIRGIYDDDERAEYRCSHHNPYIKQVYAEFLGSAGSHK
                     AHELLHTHYEARDLYLK"
     gene            3519..>3767
                     /locus_tag="VFL04_01400"
     CDS             3519..>3767
                     /locus_tag="VFL04_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7838383.1"
                     /translation="MSPLDLVLLLCLSAIWGSSFMFMRYLAPIIGPVATADARLVIAG
                     VALTALFALLGFALKWRERWRRYLVIGVLNSGLPFLLFS"
BASE COUNT          733 a         1265 c         1191 g          578 t
ORIGIN      
        1 ttctacgtcg aggtgaagcc cgaggacgcg aaggagatca tcgccgagca ggtagtgaag
       61 ggccgcgagg tgaagcgcct cctctacagg aagagcgcca agagctccga cacggtcaag
      121 gtcgaggaca tcgagttcta ccagaagcag ttccgcatag tcctgcgcaa ctgcggggtg
      181 atcaaccccg agaacatcga cgagtacata gcgcgcgagg gctacacggg cctggagcgc
      241 gccctgttca ccatgaagcc cgaggacgtg atccaggagc tcaagacctc gggcctcagg
      301 ggccgcggcg gggcgggctt ccccacctgg ctcaaatggg acctcacgat aaaggcgaag
      361 ggcgaggtca agtacgtcgt ctgcaacgcc gacgagggcg atcctggcgc ctacatgaac
      421 cgctcgacca tcgagggcga cccccactcg attctcgagg ccatgacgat ctgcggctac
      481 acgatcgggg cgaggaaggg ctacatctac atccgcgccg aataccccct cgccatcgag
      541 cgcctcaaga tcgccattaa gcaggcaagg gagtacggcc tcctcggcga ggacatcctc
      601 ggctcgggct tctccttcga cctcgagatc aggctcgggg cgggcgcctt cgtctgcggc
      661 gaggagactg ccctccttca gtccctcgag ggcaagcgcg gcatgcccgt gcccaagcct
      721 cccttccccg ccgtctcggg gctctggaag aagcccaccg tcatcaacaa cgtggagacc
      781 tacgcgaccg tcgccttcat catggccaag ggagctgaat ggttcgcgaa gatcggcacg
      841 gagaagtcga agggcacgaa gaccttcgcc ctcaccggga agatcaacaa ctccggcctt
      901 gtggaggtgc cgatgggcac caccctccgg gagatcgtgt acgacatcgg cggcgggatc
      961 aggggcggaa aggcattcaa gggcgtccag accgggggac cctcgggcgg catcatcacc
     1021 gccgagagcc tcgaccagcc gattacctac gagagcctgt cggccttggg ctcgatgatg
     1081 ggctcgggcg ggatgatcat catggacgag gacgactgcg tcgtcgacat ctccaagttc
     1141 tacatggcct tctgcgtgga cgagtcctgc gggaagtgcg cgccatgccg catcggcacg
     1201 agccagatgc acgcgatcct cgacaggata agcaagggcg ccggcaccga ggaggacctc
     1261 gccaagctcg agaagatcgg gaaggccatg accaaggcct cgctctgcct cctcggaggc
     1321 tcggccgcga acccgaccat ctcgaccctg aagcacttcc gcgaggagta cctcgagcac
     1381 atccgtgacc acagatgccg ggcggggaag tgcaaggacc tcgtggtcta cgagatcgac
     1441 gcggccaagt gcatcggctg cggcctgtgc gcgcgcaagt gccccgtgcc ctgcatcgcg
     1501 ggcgacaaga aacaggccca cgtgatcgac aaggcgcgct gcgtcaagtg cggcgagtgc
     1561 ttcaaggcct gcaagttcgg cgcggtgagc aagaagtagg cgaaggagag cccagcaacc
     1621 atgatcaacg ccaagataaa cggaatcccc gtgcaggtgg ccgagggcac gacgatcctc
     1681 gacgctgccc gcaaggtgga ggtgaagata cccaccctct gctacaacga cgacctcgag
     1741 ccctgggccg cctgcggcat ctgcgtcgtc aaggcccagg gcagcgcgaa gatgctccgg
     1801 gcctgctgca cgccgatcga cgaggggatg agcatcatca cccacgatcc cgacatcgtg
     1861 cagacccgca agaccgtgat cgagctcatc ctctccaccc accccgacga ctgcctcgcc
     1921 tgcccccgga accaggagtg cgagctccag cgcctcgccg ccgagttcgg gatccgcgag
     1981 cagcccttcc cgaaggcggt aagggagctg cccctcgacg actcgacggg gtccctcatc
     2041 ctgaaccccg agaagtgcat ccgctgcggc cgctgtgtca aggtctgcca ggacatgcag
     2101 aatgtctggg ccatcgagtt cctcggcagg ggcgagaaga cccggatcgc ccccgcggcc
     2161 gacgtcaagc tcggtgaggg accctgcatc aagtgcggcc agtgctcggc gcactgcccc
     2221 gtcggcgcga tctacgagaa cgaccagacc gccctcgtct gggacgcgct gatgaaggag
     2281 ggggccgagg ccaagacctg cgtcgtgcag atcgcgccgg ccgtccgtgt ggccctgggc
     2341 gaggccttcg gcctcgagcc cgggaccaac ctgaccagga agatctacac ggccctcagg
     2401 cgcatcggct tcgacgtcgt gttcgacacc aacttcgccg ccgacctcac gatcatcgag
     2461 gaggccacgg agttcgtgaa gcgcttcacc ggtgctgggc cgcttcccct gatcacctcc
     2521 tgctgcccgg cctgggtcga ctatctcgag aagtactacg gggacatgat ccccaacttc
     2581 tcgaccgcca agagccccca gcagatgatg ggggccatga taaagaccta ctgggccgcg
     2641 aaggccgggc tgccgagcga gaaggtgttc tcggtctcgg tcatgccctg caccgcgaag
     2701 aaattcgaga cccaccgcga tcccacgatg tactcatcgg gccaccagga cgtcgacgtc
     2761 tcgatcacga cccgggagct cgcgaggatg atcaagcagg ccggcatcga cctcgtcaac
     2821 cttcccgaat ccgagcccga cagccccatg ggcccgcact ccggcgccgg ggtgatcttc
     2881 ggggccacgg gcggagtcat ggaggccgcc ctgcgcaccg cctacaacta tgtcaccggc
     2941 gaggacctca aggacgtcaa cttcagcggc gtccggggca tctcggggat caaggaggcg
     3001 agcgtccaca tcaagggcac agaggtgagg gtcgcggtcg cccaccagat gggcaacatc
     3061 gcccaggtcc tcgacatggt gaggaaggcg aaggccgagg gaagggagac gccctggcac
     3121 ttcatcgagg tcatggcctg ccgcggcggc tgcatcggcg gcggcggcca gccctatggc
     3181 tgcaccgacg aggtgcgcaa gctgcgcatc cgcggcatct acgacgacga cgagcgggcc
     3241 gaataccgct gctcccacca caacccctac atcaaacagg tctatgccga gttcctgggc
     3301 tcggccgggt cgcacaaggc ccacgagctt ctccataccc actacgaggc gagggacctc
     3361 tacctgaaat agcctcgcat ccacagcgcg aatggcggcc gccccgatcg ggcggccgcc
     3421 attttcatct gggccctgga cgggaattgc ctgtccggtt ttcggtggaa gcgatggagc
     3481 ccgatcgagt agctttgtcc ggtgccaagg aggttccgat gtcccccctc gacctggtcc
     3541 ttttgctctg cctctccgcg atctggggca gctccttcat gttcatgcgc tacctggccc
     3601 ccatcatagg acccgtcgcc accgccgacg cgaggctcgt cattgccgga gtagccctga
     3661 cggccctgtt cgccctcctc ggtttcgccc tgaagtggag ggagcgctgg aggcgctacc
     3721 ttgtgatcgg cgtcctgaac tcgggcctgc ccttcctcct cttctcc
//
LOCUS       DASSQV010000076         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1387531, whole genome shotgun sequence.
ACCESSION   DASSQV010000076 DASSQV010000000
VERSION     DASSQV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387531"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFL04_01405"
     CDS             <1..199
                     /locus_tag="VFL04_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838384.1"
                     /translation="FFRLPGGPRPETPEPRPETTTRPQPGPQERPAAPALAAAPAERK
                     PGSSTSIVPADDAVDQVFEEF"
     gene            complement(220..1098)
                     /locus_tag="VFL04_01410"
     CDS             complement(220..1098)
                     /locus_tag="VFL04_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7838385.1"
                     /translation="MGLALLSIAAIAAQGSRIPKTLEFSRIPAVFAAYCQACHEWAGS
                     YEGIMGSGLVVPGRPEESKAWLMVAEGRMPAAGPRPSEEEKALIRDWIGAGAPRPSEA
                     GPSPGKAGPAEGQAQARFLGFPNKLAFHRFSGWMSGGLLLAAGAIGAVHAYDMMTKAH
                     DFRDANGITEETMSGVCDQEIRDVWSSPAQRRLGWTHAGLVAAGESFYIANAVTGIGY
                     IGGEGPGLSKRRLHLYAFIAHGSLMAAEVVMGFLTSSALSRGDHDTVSSLGVAHAAVG
                     LAIPIVILSAGAIMGE"
     gene            complement(1188..1637)
                     /gene="dtd"
                     /locus_tag="VFL04_01415"
     CDS             complement(1188..1637)
                     /gene="dtd"
                     /locus_tag="VFL04_01415"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET7838386.1"
                     /translation="MRAVAQRVIEARVSVDGITVGEIGRGLLVYVGVGRGDGAEDARW
                     LADKVARLRIFPDAAGVMNESALDRGASALVVSQFTLHADARKGRRPSYAEAAPPEEA
                     LPLYEAFVADLRAILGRVETGRFQEHMQVSSVNEGPVTILLDSRKAF"
     gene            complement(1637..>2235)
                     /locus_tag="VFL04_01420"
     CDS             complement(1637..>2235)
                     /locus_tag="VFL04_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838387.1"
                     /translation="ALPPLRRLLEGAPSRPEVAAVVLSGAERSAPVTTGGGFRYVLTE
                     DEALASFERAKAFFQSYRDNAALVEINRLLGSNASQGVKDKARTLKGFVGAPDFRTVR
                     DAPTMAELGRDPLLYYGCSVIWRGMAANVRPEGKALAFDFLVGYDRMKRLEGIIPARI
                     TGAEVPVDRPLEILALIRSAEGPARLDAVAIHELMAER"
BASE COUNT          330 a          797 c          778 g          330 t
ORIGIN      
        1 cttcttcagg cttccgggag ggccacggcc cgagacgccg gagccccggc cggagactac
       61 gacccggccg cagccgggcc cgcaggaaag accggcagcc ccggccctcg ccgcggcgcc
      121 cgccgagcgg aagcccggct cctcgacctc catcgtgccc gccgacgacg ccgtcgacca
      181 ggtcttcgag gagttctgag ggccgcctgg cctgggggcc tattcgccca tgatggcgcc
      241 ggcgctcagg atgacgatgg gtatcgcgag gccgacggcc gcgtgggcca cgccgaggct
      301 gctcaccgtg tcgtgatcgc cccgggatag ggccgagctc gtcaggaagc ccatgacgac
      361 ctcggccgcc atcaggctgc cgtgggcgat gaaggcatag aggtggagcc tgcgcttcga
      421 gaggccgggg ccctcgccgc ctatgtagcc gatgccggtg acggcgttgg cgatgtagaa
      481 ggactcccca gccgccacaa ggcccgcgtg ggtccagccg aggcggcgct gggcggggct
      541 gctccacaca tcgcgtatct cctggtcgca gaccccgctc atcgtctcct cggtgatgcc
      601 gttcgcgtcc cggaaatcgt gggccttcgt catcatgtcg taggcgtgga cggctccgat
      661 cgccccggcg gcgagcagga ggccccccga catccagccc gagaagcggt ggaaggcgag
      721 cttgttcggg aagccgagga agcgcgcctg ggcctggccc tcggccggac cggccttgcc
      781 cgggcttggg ccggcctcgc tcgggcgcgg ggctcccgcc ccgatccagt ccctgatgag
      841 ggccttctcc tcctcgctcg gacgggggcc cgccgccggc atcctgcctt cggccaccat
      901 gagccaggcc ttgctctcct cgggtcttcc gggaaccacg aggcccgagc ccatgatccc
      961 ctcataggag cctgcccact catggcaggc ctggcagtag gcggcgaaga cggcagggat
     1021 ccttgagaac tcgagggtct tgggtatccg ggagccctgg gctgcgatgg ccgctatcga
     1081 aaggagggcg aggcccaaaa aggccgccgt cctgggccca cttggggcgc ggcgccaacg
     1141 tttctccatg gcggaatctt acgccctcca cggccgcccc gcaagcgcta aaaggccttc
     1201 cgcgaatcga ggaggatggt gaccggcccc tcgttgaccg aggagacctg catgtgctcc
     1261 tggaagcgcc ccgtctcgac ccgccccagg atcgccctca ggtcggcgac gaaggcctcg
     1321 tagaggggca gcgcctcctc ggggggagcg gcctcggcgt aggagggcct gcggcccttc
     1381 ctcgcgtcgg cgtgcagggt gaactgggag acgacgaggg ccgatgcccc cctgtccagc
     1441 gccgactcgt tcatcactcc ggctgcgtcg gggaagatcc tgagcctggc gaccttgtca
     1501 gcgagccacc tggcgtcctc ggcgccgtcg cccctgccca cgcccacata gacgaggagg
     1561 cctcgaccta tctccccgac ggtgatgccg tcgacgctca cccgggcctc gatgaccctc
     1621 tgcgcgacgg cccgcatcag cgctcggcca tgagttcgtg gatcgcgacg gcgtcgagcc
     1681 tggccggccc ctcggcgctc cggatcaggg cgaggatctc gagggggcgg tccaccggca
     1741 cctcggcgcc cgtgatccgc gcggggatga tgccctcgag ccgcttcatc ctgtcgtagc
     1801 cgacgaggaa gtcaaaggcc agggccttgc cctcaggcct gacgttggcc gccatccccc
     1861 tccagattac cgagcagccg tagtagagca gggggtccct gccgagctca gccatagtcg
     1921 gcgcatccct gacggtgcgg aaatcggggg cgccgacgaa gcccttcagg gtcctcgcct
     1981 tgtccttcac gccctggctg gcgttcgagc cgaggaggcg gtttatctcg accagggcgg
     2041 cgttgtctcg ataggactgg aaaaaggcct tggccctctc gaaggacgcg agggcctcgt
     2101 cctcggtgag gacatagcgg aagccccctc ccgtcgtgac gggggccgag cgctcggctc
     2161 cgctcaggac caccgcagcg acctcgggcc tcgacggcgc cccctcgagg agacggcgca
     2221 ggggagggag ggcaa
//
LOCUS       DASSQV010000077         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_394595, whole genome shotgun sequence.
ACCESSION   DASSQV010000077 DASSQV010000000
VERSION     DASSQV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394595"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFL04_01425"
     CDS             <1..342
                     /locus_tag="VFL04_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255312.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HET7838388.1"
                     /translation="HDIGKNLVKMMMEGKGLKVVDLGVDVSSERFVAAAKEHGADIIA
                     CSALLTTTMTEMKAVVEGIRAAGIRERVTVMVGGAPITEAFCRSIGADLSASDAASAA
                     DAAVAVCARSA"
     gene            complement(389..1306)
                     /locus_tag="VFL04_01430"
     CDS             complement(389..1306)
                     /locus_tag="VFL04_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET7838389.1"
                     /translation="MARQRIRDALAGGRILVSDGAWGSYLYAKGLATGSCPELWCVDH
                     PGLVADIAASYVAAGADMIETNSFGGSSIRLAAQGLSSRVSELNGAAARISRAAAGEG
                     VWVIASVGPTGAMLVTEDCSEAELYDSFAEQALALAGGGADAFCVETMSDAAEAAIAI
                     RAAKEATGLEVLATFTFSRTVQGQYRTMMGLSPEEAAAAAVAAGADVVGTNCGNGYAA
                     MVDIAAAMRAAAPGVPIMVQANAGLPLIVDGALSYPEGPDYVASLVPALLDAGVGIIG
                     GCCGTTPAHIAAIRRAVDLYSAPRGPWRP"
     gene            1399..2643
                     /locus_tag="VFL04_01435"
     CDS             1399..2643
                     /locus_tag="VFL04_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET7838390.1"
                     /translation="MKRFLRAALPAIVLAGLALGAPPCVFAAPPAAPPELRVGTEDVR
                     IEARDDGGYDLYVRKKPGIASILLTETTKDPAMKADNYAYRAAEYNPVNGDEKRMLNG
                     KALPPTNRLYSLISSTPVPDKAFGSAYRVLIPAVLVYGYPWSRSGSVAVGKGTFVNIR
                     AFEKAYADYTGRFLDNPYEISISTRPPPPAPPAPPPPPPAPPVPTVVAPPAPPPPPPG
                     DYAPATVASFEAIADATKGKTAYVPEDGSTSARIAELIDGAKGDSLDLVICFDTTMSM
                     DPYMKDVKRNLAILVREKTARFSSFRVGLMLFKDYFPDEYITRKVPFMTDIDRFDSYI
                     KGLRAFGGGDIPEAVHEALYAAATEFDWKATKRIIILVGDAPPHPVPKGSITFGSVVG
                     AAMAKDIEIDALIEPVTIKGGN"
     gene            2643..3227
                     /locus_tag="VFL04_01440"
     CDS             2643..3227
                     /locus_tag="VFL04_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838391.1"
                     /translation="MPATRPRAGGAAGRAAALLFCLAAVSTSWCDGAHEEYENALRVL
                     SSRAAGPAPLPLDVVVDLALNPPDEAAAPRLLEEQKAGIASLLRDRLVGSAFSLGRVL
                     SLAELGPGLGRPAAGLGSAGAFDAKLAAACGTAAAVLLVQAEVLVSGTGGMSEITARL
                     LEAPTGRELSRDVIWTTKAEGGAGTVFVNGVRIR"
     gene            3285..>4102
                     /locus_tag="VFL04_01445"
     CDS             3285..>4102
                     /locus_tag="VFL04_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838392.1"
                     /translation="MEDSGGARLPNFWSFFILLALGLPLAAGVGDVLTGMATAGHFIA
                     SFTLAPMLSLLASGGLMALVQGLLMRRLASLGRGSFLIGSRISLLVWGPANAVLFSLL
                     FHGGCDYARDALGQTITGMYNGAVGLFFASLLVVASSDEVEALLDRGRGGQGGVLRLG
                     PKIFIAVTLAILAFLVGAIGVTLMPVYRGASISEAIRSTLVVAFPFLLLSMVLVYYLN
                     RSVAHSVGGEPQAIADLADEVASGRLGLEFAAAKREEGIYRAVKRMTGKLREVVT"
BASE COUNT          615 a         1496 c         1366 g          625 t
ORIGIN      
        1 cacgacatcg gcaagaacct cgtcaagatg atgatggagg gcaagggcct caaggttgtc
       61 gacctcggcg tcgacgtctc gagcgaaagg ttcgtcgcgg ccgccaagga gcacggggcc
      121 gacatcatcg cctgctcggc cctcctcacc acgacgatga ccgagatgaa ggccgtggtc
      181 gagggcatca gggccgcagg catccgcgag agggtcaccg tcatggtggg cggggccccg
      241 atcaccgagg ccttctgcag gtccatcggg gccgacctga gcgcctcgga cgccgcgagc
      301 gcggccgacg ccgcggtcgc ggtctgcgcg aggtcggcct gaatccgccg aggactgact
      361 aaagccgccc agagccgcct agggccgcct agggccgcca cgggccgcgc ggagccgagt
      421 acaggtcgac ggcgcggcgt atggccgcga tgtgggccgg ggtcgtcccg cagcagcctc
      481 cgatgatgcc cacccccgcg tcgaggaggg cgggcacaag tgaggcgacg tagtcggggc
      541 cctcggggta gctcagggcc ccgtcgacga tgaggggcag gccggcgttg gcctggacca
      601 tgatgggcac cccgggggcc gcggcccgca tcgccgcggc tatgtcgacc atggcggcgt
      661 agccgttccc gcagttcgtc ccgacgacat cggcccccgc cgcgaccgcg gccgcggcgg
      721 cctcctcggg cgagagcccc atcatcgtgc ggtactggcc ctggaccgtc cgcgagaagg
      781 tgaaggtcgc gaggacctcg agtccagtgg cctccttggc cgccctgatg gcgatggccg
      841 cctcggcagc gtcgctcatc gtctcgacgc agaaggcatc ggccccaccc ccggcgaggg
      901 cgagggcctg ctcggcgaag gagtcgtaaa gctcggcctc gctgcagtcc tcggtgacga
      961 gcatggcccc cgtggggccg accgaggcga tgacccagac cccctcgccc gcggccgccc
     1021 gagatatgcg cgccgcggcc ccgttgagct cggaaaccct tgaggagagg ccttgggccg
     1081 cgagcctgat gctcgagccc ccgaagctgt tggtctcgat catgtcggct cctgccgcca
     1141 catagctcgc ggcgatgtcc gcgacaagcc cgggatggtc gacgcaccag agctcggggc
     1201 atgagccggt ggcgagcccc ttcgcgtaga ggtaggagcc ccaggccccg tctgagacga
     1261 ggatcctgcc gcccgcgagg gcgtccctta tcctttgcct tgccatggcc agaggatagg
     1321 cgagaccaag gcccctgtga agtgggcccg ggttcgctaa taccccgccc cgcgcccacc
     1381 gatcattagt acgtgagcat gaagcgcttt ctgcgggcgg ccctccccgc catcgtcctc
     1441 gctggcctcg ccctcggggc cccgccctgc gtcttcgccg cgcccccggc ggcgccgccc
     1501 gagctcaggg tcggtaccga ggacgtgagg atcgaggccc gcgacgacgg cggctacgac
     1561 ctctacgtga ggaagaagcc gggcatcgcc tcgatcctcc tcaccgagac cacgaaggac
     1621 ccggcgatga aggccgacaa ctacgcctac agggccgccg agtacaaccc tgtcaacggc
     1681 gacgagaagc ggatgctcaa cggcaaggcc ctgcccccga ccaacaggct ctactccctg
     1741 atctcctcga ccccggtgcc cgacaaggcc ttcggctcgg cctaccgcgt cctcatcccc
     1801 gccgtcctcg tctacggcta tccatggagc cgttcgggct cggtggccgt ggggaagggc
     1861 accttcgtga acatcagggc cttcgagaag gcctacgccg actacaccgg ccgcttcctc
     1921 gacaacccct acgagatcag catatcgacg aggccaccgc cgccggctcc ccccgcgcca
     1981 cccccgcctc cccccgcccc gcccgtgccg acggtcgtgg cccccccggc ccccccgcct
     2041 ccgcccccgg gcgactacgc ccccgcgacg gtggcatcat tcgaggcgat cgccgacgcc
     2101 acgaagggca agacggccta cgtccccgag gacggatcga cctcggcccg gatagccgag
     2161 ctcatcgacg gggcgaaggg cgattccctc gacctcgtca tctgcttcga cacgaccatg
     2221 agcatggacc cctacatgaa ggacgtgaag cgcaacctcg cgatcctcgt gcgcgagaag
     2281 actgcgaggt tctcgagctt ccgcgtcggc ctcatgctgt tcaaggacta cttccccgac
     2341 gagtacatca cgcggaaggt ccccttcatg accgacatcg acaggttcga cagctacatc
     2401 aagggcctca gggccttcgg cggcggcgac atccccgagg ccgtccacga ggccctctac
     2461 gccgcggcca ccgagttcga ctggaaggcg accaagcgca tcatcatcct cgtgggcgac
     2521 gccccgcccc accccgtgcc gaaggggagc ataactttcg ggagcgtcgt gggggcggcg
     2581 atggcgaagg acatcgagat cgacgccctc atcgagccgg tgacgatcaa gggcggcaac
     2641 tgatgccggc cacgaggcct cgcgccggcg gagccgcggg gcgggcggcg gccctcctct
     2701 tctgcctggc agcggtctcg acctcctggt gcgatggcgc gcacgaggag tacgagaacg
     2761 ctctgagggt cctctcctcg agggccgccg gcccggcccc cctccccctc gatgtcgtgg
     2821 tcgacctcgc cctgaacccc ccggacgagg ccgccgcccc gaggctcctc gaggagcaga
     2881 aggccggcat cgcctccctg ctcagggacc gtcttgtcgg ctcggctttc agccttgggc
     2941 gcgtgctgag cctggccgag ctcggtccag ggctcggccg tccggccgca ggcctcggct
     3001 cggcgggcgc ctttgacgcc aagcttgccg ccgcctgcgg gacggcggcc gctgtcctcc
     3061 tggtgcaggc cgaggtcctc gtctccggca cgggcggcat gtcggagatc acggcccgcc
     3121 tcctcgaggc ccccacgggc agggagctgt cccgtgacgt gatctggacg acaaaggccg
     3181 agggcggggc cgggaccgtc ttcgtcaacg gggtgaggat caggtaggga gacctcattg
     3241 acagccccat gtcctcggcc atacttcaga tgactggggg caccgtggaa gattcaggcg
     3301 gggcaagact gcccaacttc tggtcctttt tcatcctcct cgctctgggc ttgcccctcg
     3361 ccgcgggagt cggcgacgtc ctcacgggca tggccacggc cggccatttc atcgcctcct
     3421 tcaccctcgc ccccatgctg agcctccttg cctccggtgg cctcatggcc ctggtgcagg
     3481 gcctcctcat gcggcggctg gcctcgctcg ggagggggag tttcctcatc ggttcccgaa
     3541 tcagcctcct ggtctggggc ccggcgaacg ccgtgctctt ctccctgctc ttccacgggg
     3601 gatgcgacta tgcgagggac gccctcggcc agacgatcac ggggatgtac aacggggccg
     3661 tggggctctt cttcgcgagc ctcctcgtgg tcgcctcatc ggacgaggtc gaggccctcc
     3721 tggatcgggg ccgagggggg cagggcggtg tgctgaggct gggtccgaag atcttcatcg
     3781 cggtgaccct cgcgatcctg gccttcctcg tcggggcgat cggcgtgacc ctcatgcccg
     3841 tctaccgcgg cgccagcatc tccgaggcca tccgcagcac cctcgtcgtg gccttcccct
     3901 tcctcctttt gtccatggtc ctggtctact acctcaaccg ctccgtcgcg cattcggtgg
     3961 gcggcgagcc gcaggccata gccgaccttg ccgacgaggt cgcctccggg aggctgggtc
     4021 tggagttcgc cgccgccaaa cgcgaggagg ggatctaccg cgccgtcaag cgcatgacgg
     4081 gcaagctcag ggaggtggtg ac
//
LOCUS       DASSQV010000078         4965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1762640, whole genome shotgun sequence.
ACCESSION   DASSQV010000078 DASSQV010000000
VERSION     DASSQV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762640"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFL04_01450"
     CDS             complement(<1..377)
                     /locus_tag="VFL04_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7838393.1"
                     /translation="MRKAIVLALFATVFLVGPMGAFAQAKPVDIELWALGAVTEAGNP
                     PDNWVGYQIIRDKLGINLKLVLEPSTPSDQDTKISVAAAANSLPDLFGVNRDMLMKIA
                     KAGLVAQVDGLLPKMPIRTKTHYS"
     gene            complement(425..1300)
                     /locus_tag="VFL04_01455"
     CDS             complement(425..1300)
                     /locus_tag="VFL04_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7838394.1"
                     /translation="MKKLRWGEELRFQLLTNAFLVIVLLIVLLPLWRVLVLSVTPFRH
                     VDRSFGLLVSPSVWTFEAYSQFLSHPAFLHAFRNSLIITFGGVAISLFMTVPMSYAYS
                     IRTLPGRKALNLFAMLPFLFNPGLIPSYLVVTGLGLQDNLLAVMLPPAVGIANMFIMR
                     NFFQEIPQDLKEAARIDGAGELFILMRIIIPLSMPILLTIGLFYAVGHWNEFFTPLLY
                     LNDAKLQPLPVLLRNILMAVNMNEYIEYDAFSSSSVQSLKAASIFITMVPMMMAYPWI
                     QKHFVKGSLAGGIKG"
     gene            complement(1310..2245)
                     /locus_tag="VFL04_01460"
     CDS             complement(1310..2245)
                     /locus_tag="VFL04_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7838395.1"
                     /translation="MRRRRNRGERRRQLFRNLRRHAVFYAMLALPIAYFLVFKYGPLV
                     NAQIAFKDFSPLDGVWKSPWVGFKNFLTFFSSIYFWELLRNTIGLSLGKLLLGLPLAV
                     VLAVALNESVLRRFKRLVQTITYLPHFLSWVVMYGVLLLLLSPDEGLVNQVIKAAGGQ
                     PIGFLYTPSWYPAVVVLSDVWKEVGWSAIIYLAAITGIDPGLYEAAEVEGATRMQRIR
                     YITLPGILDVVITIGLLRLGNVLDAGFHQIFVTYSVPVYSVGDIIDTWVYRQGVLDFQ
                     FSLATAVGLFKGCIGLALVVSFNRLARRYTDSGLF"
     gene            complement(2369..3424)
                     /locus_tag="VFL04_01465"
     CDS             complement(2369..3424)
                     /locus_tag="VFL04_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET7838396.1"
                     /translation="MQQLLSSLPLDPETLRWLVALAYAVAGYVGGKLLSVVITGVLKR
                     LAAKTKNRVDDIILAVTEKPLVFIVFVAGLWFGIDSLDLDVSVTHWVDKVFYVLVTFA
                     VAWILVRLIDGIINEYLAPFVAKSESSLDDQLLPIIRKVVKVLIWILATLVGLNNAGY
                     DVGALLAGLGIGGVAVALAAKDTLSNLFGSIAVFIDRSFAVNDRIKVAGFDGTVTEIG
                     LRTSRLRTLDNRVITIPNAIFGTNPIENVSSEPSTKVGQVLELSLDNDAGAVEKGLDI
                     LRTVASGFGTSLDEGTIAAFSGFGEGTFRLSFIVFLKKGADYFATLSALNLAVLKAFK
                     EAGIEFARPARLGGGPR"
     gene            3513..4397
                     /locus_tag="VFL04_01470"
     CDS             3513..4397
                     /locus_tag="VFL04_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7838397.1"
                     /translation="MERLYLGSCSWKYPSWAGLVYSGPSPADFLAEYASKYDSVEIDQ
                     WFWSLFKGKPPVLPRRELAASYASSVPEHFRFTVKCPNALTLTETHVQGGRAGSPNPD
                     FLDPGLFGRFLEAIEPILPKVGLFIFQFEYFNARKMPERAALLDRLGPFLEALPRGLP
                     YGVELRNPRWLDPAWFSFLEERRAAPVFLQGYWMDDVAALYARIFGPRGPLGRAVPKT
                     VCLRLHGEDRQGIEAEAGEDWSRILRDRDEELPPLMAVVRDLLGRGSTVFINVNNHYE
                     GSAPITIDKINRLLTAGP"
     gene            4430..>4965
                     /locus_tag="VFL04_01475"
     CDS             4430..>4965
                     /locus_tag="VFL04_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET7838398.1"
                     /translation="MIEVFVAPFTANAFMANALLAGLLVSLACGIVGTFVVLRGLAFI
                     GDALAHGVLPGVAIALLLGLPGMLGAAAGSLAMIAGIGLVTKKSSLSSDTAIGLLFVG
                     MLALGVTIVSRSRAFSGDLVRILFGEILGISHSEILMQGGATLAIAATAFVCYRPFLL
                     LCFNPEQADVSGFSSRLY"
BASE COUNT          870 a         1567 c         1668 g          860 t
ORIGIN      
        1 gagtagtggg tcttcgtgcg gatgggcatc ttcggcagga ggccgtcgac ctgggccacg
       61 aggccggcct tcgcgatctt catgagcatg tcgcggttca cgccgaagag gtcgggcagg
      121 gaattggctg ccgcggccac gctgatcttc gtgtcctggt cgctcggggt cgagggctcg
      181 aggacgagct tgaggttgat ccccagcttg tcgcggatga tctggtagcc gacccagttg
      241 tcggggggat tccccgcctc ggtcacggcg ccgagggccc agagttcgat gtcgaccggc
      301 ttggcctggg cgaaggcgcc catcggcccg acgaggaata ccgtcgcgaa gagcgcgaga
      361 acgatcgcct ttctcatgca gtgcctcctt taggatgact ttgtgacatg gcgcccccgg
      421 ggattcagcc cttgatgccg cccgcgaggg agcccttgac gaaatgcttc tggatccacg
      481 gatacgccat catcatgggg accatggtta tgaagatgct cgccgccttg agcgactgca
      541 ccgagctcga gctgaaggcg tcgtactcga tgtactcgtt catgttgacc gccatgagga
      601 tgttgcgcag gaggacgggc aggggctgga gcttggcgtc gttcaggtag agcaggggcg
      661 tgaagaactc gttccagtgg cccaccgcat agaagagccc gatcgtgagc aggatcggca
      721 tggagagggg gatgatgatc ctcatgagta tgaagagctc ccccgcgccg tcgatcctgg
      781 ccgcctcctt gaggtcctgg ggtatctcct ggaagaagtt ccgcatgatg aacatgttgg
      841 cgatgccgac ggccggcggg agcatgaccg cgagcaggtt gtcctggagg cccaggcccg
      901 tgacgacgag gtaggagggg atgaggccgg ggttgaaaag gaagggcagc atcgcgaaga
      961 ggttcagggc cttcctcccg gggagggtcc ggatcgagta ggcgtaggac atcggcaccg
     1021 tcatgaagag gctgatggcc accccgccga aggtgatgat caggctgttg cggaaggcgt
     1081 ggaggaaggc cggatggctc aggaactggg agtaggcttc gaaggtccag accgaggggc
     1141 tcacgaggag gccgaagctc ctgtccacgt ggcggaaagg cgtcacggag agcacgagga
     1201 cccgccagag cggcaggagc actataagga gcacgatgac caggaaggcg ttggtcagca
     1261 gctggaacct gagctcctcg ccccacctga gtttcttcat ccggccctcc tagaacaagc
     1321 ccgaatcggt gtagcgcctg gcgagcctgt tgaaggacac taccaaggcg aggccgatgc
     1381 agcccttgaa gaggccgacg gctgtggcga ggctgaactg gaaatccagg acgccctgac
     1441 ggtagaccca ggtgtcgatg atgtcgccga cgctgtagac cggcaccgag taggtcacga
     1501 agatctggtg gaagcccgcg tcgagcacgt tgccgagcct gaggaggccg atcgtgatca
     1561 ccacgtcgag gatgcccggc agggtgatgt agcggatcct ctgcatcctc gtcgcgccct
     1621 cgacctcggc ggcctcgtat agccccgggt cgatccccgt gatcgcggcg aggtagatga
     1681 tggcgctcca gcccacctcc ttccacacgt cgctgaggac gacgacagcc gggtaccagc
     1741 tcggggtata gaggaagccg atcggctggc cgcccgcggc cttgatcacc tggttcacga
     1801 ggccctcatc tggggagagg agaaggagga ggaccccgta catgaccacc caggagagga
     1861 agtggggcag gtaggtgatg gtctggacga gtcgcttgaa gcgcctgagg accgactcgt
     1921 tgagggccac cgcgagcacg acggccaggg gaaggccgag gaggagcttg ccgagggaga
     1981 gccctatcgt gttccgcagg agctcccaga aatagattga ggagaagaag gtcaggaagt
     2041 tcttgaagcc gacccagggg ctcttccaga ccccgtccag ggggctgaaa tccttgaagg
     2101 cgatctgggc gttgaccagg gggccgtact tgaacacgag gaaataggcg atggggaggg
     2161 ccagcatggc gtagaagacc gcatgcctgc gcaggttcct gaacagctgt cgcctcctct
     2221 cgccccggtt tcgccggcgg cgcactgctg cagcgccgcc ggtgagacca gtatctgccc
     2281 gaaaggccgg gaaaagaccg cccaggtttt ggacttttcc ggcctcaggc ccgagcccca
     2341 ggctagggcc tccggcctgg gccccgggct acctggggcc tcccccgagg cgggcggggc
     2401 gggcgaactc gatccccgcc tccttgaagg ccttgagcac ggcgaggttg agcgcgctca
     2461 gggtggcgaa atagtcggcg cccttcttca gaaagacgat gaagctgagg cggaaggtcc
     2521 cctcgccgaa gccgctgaag gccgcgatgg tcccctcgtc gaggctggtc ccgaaaccgg
     2581 aggccacggt cctcaggatg tcgaggccct tctccacggc ccccgcgtcg ttgtccagcg
     2641 agagctcgag gacctggccc accttggtcg agggctccga gctcacgttc tcgatggggt
     2701 tcgtgccgaa gatggcgttg ggtatcgtga tgaccctgtt gtcgagggtc ctcagcctcg
     2761 aggtccgcag ccctatctcg gtgacggtcc cgtcgaagcc cgcgaccttg atgcggtcgt
     2821 tgaccgcgaa ggagcggtcg atgaagacgg cgatgctgcc gaagaggttg gacagggtgt
     2881 ccttcgcggc gagggcgacg gcgacaccgc cgatgccgag gccggcaagg agggccccga
     2941 cgtcgtagcc ggcgttgttg agccccacga gggttgcgag gatccagatc agcaccttga
     3001 cgaccttcct gatgatcgga aggagctggt cgtcgaggct gctctcgctc ttggcgacga
     3061 agggggcgag atactcgttg atgatcccgt cgatgagcct gacgaggatc cacgcgacgg
     3121 cgaaggtcac gaggacatag aagaccttgt cgacccagtg ggtcacgctg acgtcgaggt
     3181 cgagcgagtc gatcccgaac cagagcccgg ccacgaagac gatgaacacg aggggcttct
     3241 cggtcaccgc gaggatgatg tcgtcgactc ggttcttggt cttggcggcg aggcgcttga
     3301 gcacgcccgt tatcactacc gagaggagct tacccccgac gtagccggcg accgcgtagg
     3361 cgagggccac gagccacctg agggtctcag ggtcgagcgg gagggatgac agaagctgtt
     3421 gcatggtttc tctccaaatc ctggcgctga tgtgaacgaa ggatgccaaa acctgccgcc
     3481 cttgggcaag aggaggcagt atactttccc agatggaaag gctctacctc ggcagctgct
     3541 cgtggaaata cccctcctgg gccggccttg tctattcggg gccctcgccg gccgacttcc
     3601 tggccgagta cgcctcaaaa tacgacagcg tcgagatcga ccagtggttc tggtccctgt
     3661 tcaagggcaa gccccccgtc ctgccccggc gggagctcgc ggcctcctac gcgtcctcgg
     3721 tccccgagca tttccgtttc acggtgaaat gcccgaacgc cctcaccctc acagagaccc
     3781 acgtccaggg cggcagggcg ggttcgccca acccggattt cctggacccc ggcctcttcg
     3841 ggcgtttcct cgaggccatc gagcccatcc tgccgaaggt aggcctcttc atattccagt
     3901 tcgagtattt caacgcgagg aagatgcccg agcgcgccgc cctccttgac cggctcggcc
     3961 ccttcctcga ggccctgccc cggggcctcc cctacggcgt ggagctgcgc aatccccgct
     4021 ggctcgaccc ggcctggttc tccttcctgg aggagcgccg ggccgcgccc gtgttcctcc
     4081 agggctactg gatggacgac gtggccgccc tctatgcccg catcttcggc ccgcgcgggc
     4141 ccttggggcg ggccgtgccg aagaccgtct gcctcaggct ccacggcgag gacagacagg
     4201 ggatcgaggc cgaggcgggg gaggactgga gcaggatcct cagggaccgg gacgaggagc
     4261 ttccgcccct catggccgtc gtgcgggacc tcctcggccg gggctccacg gtcttcatca
     4321 acgtcaacaa ccactacgag ggctcggcgc cgatcaccat cgacaagatc aaccgcctcc
     4381 tcacggccgg tccttgatcg gcttctcggc ctgctatatc gtgctgggga tgatcgaggt
     4441 attcgtcgcg cccttcacgg ccaacgcctt catggccaac gccctcctag ccggcctcct
     4501 ggtctccctc gcctgcggca tcgtggggac cttcgtggtc ctccgcggcc tcgccttcat
     4561 cggcgacgcc ctggcccacg gcgtcctccc cggcgtcgcg atcgccctcc tcctcggcct
     4621 gccggggatg ctcggggccg ccgccgggag cctcgccatg atcgccggca taggcctcgt
     4681 caccaagaag tccagcctct cctccgacac ggccatcggc ctcctcttcg tggggatgct
     4741 cgccctgggc gtgaccatcg tctcgcgctc ccgggccttc tccggcgacc tcgtgaggat
     4801 actcttcggc gagatcctcg ggataagcca ctccgagatc ctgatgcagg gaggggcgac
     4861 cctcgccata gccgccacgg ccttcgtctg ctaccggccc ttcctcctcc tctgcttcaa
     4921 ccccgaacag gccgacgtct ccggcttctc ctcgaggctc tacca
//
LOCUS       DASSQV010000079         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_395273, whole genome shotgun sequence.
ACCESSION   DASSQV010000079 DASSQV010000000
VERSION     DASSQV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395273"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VFL04_01480"
     CDS             <1..927
                     /locus_tag="VFL04_01480"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET7838399.1"
                     /translation="SGFLSALFVNDTVCIMLTPLVAELALRSRRDPLPYLIALATSAN
                     VGSCATIIGNPQNMLIGAQSGIGFVRFFLSLAPASLGGLALCGLFIVLAHPKEFARRE
                     RLFHVAAPRPMTYRPLLYKSLAAVALLLLALLLGLDPAIASLSAAAILLVTRRVDTGK
                     VFAEVDFSLLVFFAGLFVLTRAVEATAAFKWCVDRSLLLIAGGDGRPSLLPFSAMTAV
                     FSNLVSNVPAVMLFRPLVPLFASQDRAWLILAMASTFAGNLSLLGSVANLIVAEGAKR
                     SGIRLGFGAYLRLGLPITIASLSLGTAWLLLF"
     gene            984..2192
                     /gene="xseA"
                     /locus_tag="VFL04_01485"
     CDS             984..2192
                     /gene="xseA"
                     /locus_tag="VFL04_01485"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET7838400.1"
                     /translation="MTESREHVFGVSELTSRIGSLLEGSFPAVTVEGEISNCRPASSG
                     HLYFSLKDKASVIQAVMFRYRTRGLAFEPADGLLVRARGAVTVYAARGQYQILVERLE
                     KAGVGDILAMLEQRKRALAAEGLFDEDRKRPLPRSPERIAVITSPTGAAVRDILNVLS
                     RRNAGLGVVILPAAVQGDEAPAQIVRQIQVANRWRLGDVIILGRGGGSLEDLLAFSDE
                     AVVRAVAASAIPVISAVGHEVDWALTDFAADMRAPTPSAAAELVSESRESLARQVAQF
                     RGELETAVASRLDRARLLLGRFDPRAVEAQFMRILLPAVRSFDEARDDLARGMTDAIA
                     AMRRRIELASRDLAATSPEAVLARGFAVVTPHAGGPAIRDSAGLGPGAELDIRFARGS
                     AAARVLEARK"
     gene            2189..>2357
                     /gene="xseB"
                     /locus_tag="VFL04_01490"
     CDS             2189..>2357
                     /gene="xseB"
                     /locus_tag="VFL04_01490"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET7838401.1"
                     /translation="MKKFEERLGRLEELAERIKEPDLPLEDAVAVFEEGVKLAKGLER
                     DLDRIQGRVEVL"
BASE COUNT          318 a          885 c          743 g          411 t
ORIGIN      
        1 tcgggcttcc tgtcagcgct cttcgtcaac gacacggtct gcatcatgct cacgcccctg
       61 gtggccgagc tcgccctccg ctcccgccgc gaccccctgc cctatcttat cgcgctcgct
      121 acctcggcca acgtgggctc atgcgcgacc atcatcggca acccgcagaa catgctcatc
      181 ggcgcgcaga gcggtattgg cttcgtccgt ttcttcctca gcctggcccc cgcctcgctc
      241 ggtggcctcg cgctctgcgg tctcttcatc gtccttgccc acccgaagga gttcgcgaga
      301 cgcgagcgcc tcttccatgt cgcggccccc cggcccatga cttataggcc cctgctgtac
      361 aagagcctcg cggccgtggc cctcctcctc ctcgccctcc tccttggcct cgatccggcc
      421 atagcctcgc tctccgcggc ggccatcctg ctggtcacga ggagggtgga caccgggaag
      481 gtcttcgccg aggtggactt ctccctcctg gtcttcttcg cggggctctt cgttcttacg
      541 cgcgcggtcg aggccacggc ggccttcaag tggtgcgttg accgcagcct cctgctcatc
      601 gcgggcggcg acggccgccc ctcgctcctg cccttctcgg ccatgacagc cgtcttctcc
      661 aacctggtgt ccaacgtgcc cgcggtcatg ctcttccgtc ccctggtgcc gctcttcgcg
      721 tcgcaggaca gggcctggct catcctcgcg atggcgagca ccttcgccgg gaacctgagc
      781 ctcctcggtt cggtagccaa cctcatagtc gccgaaggag cgaagcggtc gggcataagg
      841 ctgggctttg gggcatacct gaggctgggt ctgccgataa ccatcgcgag cctttccctc
      901 ggcactgcct ggcttctcct gttctagccc cctcaagccc gcacccgttg cgccttccct
      961 gcccaagggg ctatggtagg cccgtgaccg aatccaggga acacgtgttc ggcgtctcgg
     1021 agctcacctc gcgcatcggc tccctccttg agggcagctt ccccgccgtg acggtggagg
     1081 gcgagatatc gaactgccgg ccggcgagct ccggccatct ctatttcagc ctgaaggaca
     1141 aggcctcggt gatccaggcc gtgatgttcc gctaccgcac ccggggcctg gccttcgagc
     1201 cggccgatgg cctcctcgtg cgcgcgcggg gagccgtcac ggtctacgcc gcccgcggcc
     1261 agtaccagat cctcgtcgag cgtctcgaga aggccggggt gggcgacatc ctcgccatgc
     1321 tcgagcagcg caaacgcgcc ctcgccgccg aggggctctt cgatgaggac cgcaagcgcc
     1381 cgctcccgcg ttcccccgaa cggatcgccg tcataacctc gccgacgggc gccgccgtcc
     1441 gcgacatcct caatgtcctc tcgcggcgca acgcgggcct cggggtcgtc atcctccccg
     1501 ccgcggtcca gggcgatgag gcgcccgccc agatcgtgcg ccagatccag gtcgcgaacc
     1561 gctggaggct cggcgatgtg atcatcctcg gccgcggcgg gggctcgctc gaggacctcc
     1621 tcgccttctc cgacgaggcc gtcgtgcggg cggtggccgc ctcggcgata cccgtcatca
     1681 gcgccgtcgg acatgaggtg gactgggccc tcaccgactt cgccgccgac atgagggcgc
     1741 cgacgccgag cgccgccgca gagctggtct cagagtcccg cgagagcctg gcgcggcaag
     1801 tagcccagtt caggggcgaa ctcgagacgg ccgtcgcctc ccggctcgac cgcgcccgcc
     1861 tcctcctcgg ccgtttcgac ccgcgggccg tcgaggccca gttcatgcgc atactcctgc
     1921 cggccgtcag gagcttcgac gaggccaggg acgatctggc ccgcgggatg accgatgcca
     1981 tcgccgccat gcgccggagg atcgagctcg cctcgcgcga cctcgccgcc acaagccccg
     2041 aggcagtcct cgctcggggc ttcgccgtgg taactcccca tgcgggaggc ccagcgatac
     2101 gcgattccgc cggtcttggg cccggcgctg aacttgacat acgtttcgcg cgcggctcgg
     2161 ccgctgcgcg ggtcctggag gccagaaagt gaagaagttc gaagagcggc tcggccgact
     2221 cgaggagctc gccgagcgga tcaaggagcc cgacctgccc ctcgaggacg cggtcgcggt
     2281 gttcgaggag ggcgtcaagc tcgccaaggg cctcgagcgc gaccttgaca ggatacaggg
     2341 cagggtcgag gtcctcc
//
LOCUS       DASSQV010000080         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2260368, whole genome shotgun sequence.
ACCESSION   DASSQV010000080 DASSQV010000000
VERSION     DASSQV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2260368"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFL04_01495"
     CDS             <1..580
                     /locus_tag="VFL04_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7838402.1"
                     /translation="GAFTARRVATVEGLETQFAVNHLAAFLLTTSLLPALERGSRVIT
                     VSSDSHHAGRIRWADPLMERRYFGLSAYCQSKLANVLFSRELVRRLGDASKVEVFIAD
                     PGLANTDMGLKQGLSPSSLFWRLRRRGGTSPDVPAAGIAWLAASDQVSGKTGRYWLNG
                     VEKSPSRRGRDLESARRLWELSEELCRRALRG"
     gene            complement(631..1035)
                     /locus_tag="VFL04_01500"
     CDS             complement(631..1035)
                     /locus_tag="VFL04_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7838403.1"
                     /translation="MGEELVRAMYETLPVEITVIDGEDKVVSWNRHDNRLFYRPEVSM
                     GMNFRECHPQESLHLVERIVGDMKSGKRERARFWIDLTVDKKSGLKHKVLIDFYALRN
                     DAGAYMGCMECNLDVEEIRHLEGERRLLDEQP"
     gene            complement(1267..2001)
                     /locus_tag="VFL04_01505"
     CDS             complement(1267..2001)
                     /locus_tag="VFL04_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HET7838404.1"
                     /translation="MKIAIDTHTHSLASGHAYSTVDEMARGARRRGLKGFVLTDHGPG
                     ISGGIQPHPYHFGNLPIIPERIRGIRFLKGVEANILDLEGSLDMAERQLARLDFVLAG
                     LHEACFAPRGTEENTAALVRALGHPHVDAVSHAGNPVYPIDARTVILAARDNGKAIEI
                     NNSSFRVRPGSHERCREIALLCMETGTPVVCGSDAHFWTDVGRFDKVMELFREIRMPA
                     ELVINSSERSFDSFLARRRADRAPAT"
     gene            complement(1998..2405)
                     /locus_tag="VFL04_01510"
     CDS             complement(1998..2405)
                     /locus_tag="VFL04_01510"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HET7838405.1"
                     /translation="MAFIEWSNSLSVGLAEIDDQHRRLIGLINRLSEVSEAGEAPASV
                     SAALGELESYVATHFALEEGYFDRFQYPAAKEHKAEHLAFAQRVASLKAEFLAGKSGL
                     SRELLPFLRSWLTNHIAFSDRKYKPLFAQKGVR"
BASE COUNT          448 a          883 c          966 g          482 t
ORIGIN      
        1 gggggccttc accgcaagac gggtggcgac ggtcgagggg cttgagaccc agttcgccgt
       61 caaccacctc gcagcattcc tcctcaccac gagcctcctc cccgcccttg agcgggggag
      121 ccgggtcatc acggtgtcct cggactcgca ccatgcgggg aggatccgct gggccgaccc
      181 cctcatggag cggcgctact ttggcctctc ggcctattgc cagtccaagc tcgcgaacgt
      241 cctgttctcc cgggagctgg tgcggcgcct tggcgatgcc tcgaaggtcg aggtcttcat
      301 cgccgatccg gggctcgcca acaccgacat gggcttgaag cagggccttt cgccgagcag
      361 cctcttctgg cggctgcgca ggaggggcgg cacgagcccg gatgtgccgg ccgcggggat
      421 cgcctggctg gcggcctccg accaggtcag tggaaagacg ggccggtatt ggcttaatgg
      481 cgtggagaaa tccccctcaa ggcgcggccg tgacctcgaa tcggcccggc ggctctggga
      541 gctcagcgag gagctctgcc gccgcgccct ccgcggctga ggcttggctc cccgggctcg
      601 cagctcgggg cccgcggcgg gagccggggc tcagggctgc tcgtcgagga ggcgccgctc
      661 cccttcaagg tgcctgatct cctcgacgtc gaggttgcac tccatgcagc ccatgtaggc
      721 gcccgcgtcg ttccgcaggg cgtagaaatc gatgagcacc ttgtgcttga gcccgctctt
      781 cttgtcgacg gtcaggtcga tccagaaacg ggcacgctcg cgcttccccg atttcatgtc
      841 gcccacgatc cgctcgacca gatggaggct ctcttggggg tggcattcgc ggaaattcat
      901 gcccatgctg acttccggcc ggtagaaaag gcggttgtcg tgcctgttcc agctcacgac
      961 cttgtcctct ccgtcgatga cggtgatctc gacgggaagg gtctcgtaca tcgcgcggac
     1021 gagttcttcg cccatggaat cgatcatggc gtggcctccg gtctggtgct acggagatta
     1081 tggtcgccgg cgtcatcccg cgccagcatc ggagcgcggg taggcatcag agagcaggaa
     1141 tcggggctcg gggaccgggg ctcggggaca gagctcgagc agggcggagc tcgagcaggg
     1201 cgacgggcac aatgtcttcc cggcccgcgg attcggggac agagccgggg cctggcctga
     1261 tgccacctag gtggccgggg cccgatcggc tcggcgcctt gcgaggaagc tgtcgaagga
     1321 acgctcggag gagttgatga ccagctcagc gggcatgcgg atctcccgga agagctccat
     1381 gaccttgtcg aagcggccga cgtcggtcca gaagtgggca tcgctgccgc agaccaccgg
     1441 ggtccccgtc tccatgcaga gtagggcgat ctcccggcag cgctcatggc ttcccggcct
     1501 gaccctgaag gagctgttgt tgatctctat ggccttgccg ttgtccctcg cggcgaggat
     1561 cacggtccgc gcatcgatgg gatagacggg gttcccggcg tgggagaccg catccacatg
     1621 gggatgcccg agggcccgaa ccagggccgc ggtgttctcc tccgtgcccc tgggagcaaa
     1681 gcaggcctcg tggagcccgg ccagcacgaa gtcgaggcgg gctagctggc gctcggccat
     1741 gtcgaggctg ccctcgaggt cgaggatgtt ggcctcgacg cccttgagga agcggatccc
     1801 gcgtatccgc tcggggatga tggggaggtt gccgaagtgg taggggtggg gctggatccc
     1861 ccccgagatg cccggcccat ggtcggtcag gacgaaacct ttgaggccac gccgtcgggc
     1921 gccgcgcgcc atctcgtcga cggtcgaata ggcgtggccc gaggcgagag agtgcgtgtg
     1981 cgtgtctatc gcaatcttca acggacgcct ttctgcgcga acaggggttt gtatttgcgg
     2041 tcggagaacg cgatgtggtt agtcagccac gaacgcagga aaggcaggag ctccctcgag
     2101 agcccgctct tgccggccag gaactcagcc tttaagctgg cgaccctctg agcgaaggcc
     2161 aggtgttcgg ccttgtgctc cttggcagcg ggatactgga atctgtcgaa gtacccctcc
     2221 tcgagggcga aatgggtcgc tacatacgac tccagctccc cgagtgccgc agagacgctg
     2281 gccggcgcct cccccgcctc ggagacctcg ctcagtcggt ttatgagccc gatgagcctg
     2341 cggtgctggt cgtcgatctc cgcgagtccg acgctgagcg agttggacca ttcaatgaag
     2401 gccatggaac ctcccgggct ggcttggagc ccctgtcgcg ccaaccaagt ggtaacccga
     2461 ggctggggac gatgtcaaag gggcagggag cgcctgccgg gccgccgaag tccggcggcc
     2521 cggccgacct ccttggggaa acgggcactg atccggggac agagctagtc gccacttggc
     2581 ccccgagctc tcctcgggcc gggctccgga gggtcttcgt tccagagcca caggggacag
     2641 agctcaaaga gagctcaaag agagctcaaa gggagctcaa agggagctcg gggactggtc
     2701 cgaggcccta gtcgccgccg ccgatctcta ggaagctgct cggtacgccc aggctccgct
     2761 tcccaagctg gacgttcag
//
LOCUS       DASSQV010000081         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2385435, whole genome shotgun sequence.
ACCESSION   DASSQV010000081 DASSQV010000000
VERSION     DASSQV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385435"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFL04_01515"
     CDS             complement(<1..827)
                     /locus_tag="VFL04_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET7838406.1"
                     /translation="MKGYSGVILHVDLGSGKFSEERPDEAFYRKYVGGGAMGAYYLLT
                     MMKPGVDALDPANVLVFSLGPITGAPLSGSARHCVTTKSPQTGGIATGEAGGYWAPEL
                     RFAGFDAIVITGRAPRPVYLWIKDGHYELRDASPIWGKVTGEAQDSIRAELGDEKVRV
                     ALIGPAGERLVRYAGIVNELKHFNGRSGVGAVMGSKNLKAIAVRGTRKPEFQDPERLS
                     ELGRLGAKRVAEDAGGAGNWKKYGTTLNVRWNTDLGGLPTRNWTMGQFEGAEGLTAEV
                     "
     gene            complement(868..1893)
                     /locus_tag="VFL04_01520"
     CDS             complement(868..1893)
                     /locus_tag="VFL04_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7838407.1"
                     /translation="MEKALDPARASEAQVSGARTGGARRLGGRGGFREGTTILVILGL
                     GLIMTFLSPYFLTVENFRTMFMSFAINGMVVIAMTLALISGGIDLSVGAVMALVGIVV
                     GTLFLNLHMNIWLAALIGIGLGLGIGLINGLFITKIGLSPFITTLGMMQVGYGVASVV
                     SQGMPLPLQSLPASFKFIGRGMIGGTGMPFVILLFLVVVFAFDFLSRRATIIRKIYYV
                     GSNEKSARFSGINVNKVRLGVYMLVGALAAFAGILSIARFNSATNTLATTVNMDAISA
                     AVIGGASLNGGEGTIFGSVLGIALLALIQTSLNLMDVGPYWQSLVTGLVLLIAVSLDF
                     LSHRKSR"
     gene            complement(1902..>2459)
                     /locus_tag="VFL04_01525"
     CDS             complement(1902..>2459)
                     /locus_tag="VFL04_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7838408.1"
                     /translation="RDAIGAGLVYLTENRALEGLFLDLSIAKNISVMDLSLVASLGLI
                     QARAETELAERYSGEMQIKLANVAQKANSLSGGNQQKVLISKLLSVEPKVVIMDEPTR
                     GIDIGSKAQIYHLLRRLASEGIGVIMISSELPEIIGVCDRVAVMYEGRICGILEGDDV
                     NERSIIQMACLSSEERETQATGAAL"
BASE COUNT          407 a          773 c          860 g          419 t
ORIGIN      
        1 acctcggccg tgaggccttc cgccccctcg aactggccca tggtccagtt gcgggtgggc
       61 aggccgccga ggtcggtgtt ccagcgcacg ttgagcgtcg tcccgtactt cttccagttg
      121 cccgccccgc ccgcgtcctc ggccacccgt ttagcgccga gccggccgag ctccgacagg
      181 cgctcggggt cctggaactc gggcttccgc gtcccgcgga cggcgatggc cttcaggttc
      241 ttggatccca tgacggcccc gacgcccgag cggccgttga agtgcttaag ctcgttgacg
      301 atgcccgcgt agcgcacgag gcgctcgccc gctggcccga tgagggccac gcggaccttc
      361 tcgtcgccga gctcggcgcg gatcgagtcc tgggcctcgc cggtcacctt gccccagatc
      421 ggtgaggcgt cgcggagctc gtagtgtccg tccttgatcc agaggtagac gggcctcgga
      481 gccctgccgg tgatgacgat ggcgtcgaag ccggcgaagc gcagctcggg ggcccagtag
      541 cctcccgcct cgcccgtcgc gatgcccccg gtctgggggg acttggtggt gacgcagtgc
      601 cgggccgagc cgctcaaggg ggcccccgtg atggggccca gggagaagac gaggacgttg
      661 gccggatcga gggcgtcgac cccgggcttc atcatcgtga ggaggtagta ggcgcccatc
      721 gcgcccccgc ccacgtactt gcggtagaag gcctcgtctg gcctctcctc gctgaacttc
      781 ccgctgccga gatcgacgtg taggatgacg ccggaatagc ccttcatgtg catgcctccc
      841 tagtttcctt ggtgttcctg cggcccctca cctggacttc cggtgcgaga ggaagtcgag
      901 tgagacggcg atgagaagga cgaggcccgt cacgagggac tgccagtagg gcccgacgtc
      961 catgaggttg agcgaggtct ggatgagggc cagaagcgct atgccgagga cggagccgaa
     1021 gatcgtcccc tcgcccccgt tgaggctcgc cccgccgatg accgccgccg agatcgcgtc
     1081 catgttcacc gtggtcgcga gggtgttcgt ggccgagttg aagcgggcga tcgagaggat
     1141 gccggcgaag gccgcgaggg cacccacgag catgtagacc ccgagcctca ccttgttgac
     1201 gttgatgccc gagaaacgcg ccgacttctc gttcgagccg acgtagtaga tcttgcggat
     1261 gatcgtggcc cggcgcgaga ggaagtcgaa ggcgaagacc acgacgagga aaaggaggat
     1321 cacgaagggc atcccggtgc cgcctatcat gccgcgtccg atgaacttga aggaggccgg
     1381 caggctctga aggggcaggg gcatgccctg ggacacgacc gaggccacgc cgtagcccac
     1441 ctgcatcatg cccagggtcg tgatgaaggg tgacaggccg atcttcgtga tgaagaggcc
     1501 gttgatgagg ccgatgccga gcccgaggcc gatgccgatg agggcggcga gccagatgtt
     1561 catgtgcagg ttgaggaaga gggtgcccac cacgatgccg acgagggcca tgacggcccc
     1621 cacggagagg tcgatgcccc ccgagatcag ggccagggtc atggcgatga cgaccatccc
     1681 gttgatggcg aagctcatga acatggtcct gaagttctcc accgtgagga agtaggggga
     1741 gaggaaggtc atgatgaggc cgaggcccag gatgacgagg atcgtcgtgc cctcgcggaa
     1801 cccgcccctg ccgccgaggc gccgcgcgcc gcccgtcctt gcgccggaga cctgggcctc
     1861 ggatgccctg gcggggtcca aggccttttc catgctcgca ttcatagtgc cgctcctgtg
     1921 gcttgggttt cgcgctcttc gctggacaga caggccatct ggatgatgga cctctcgttc
     1981 acgtcatcgc cctcgaggat cccgcagatc ctgccctcgt acatgacggc cacgcggtcg
     2041 cagacgccga tgatctcggg cagctccgat gagatcatga tgaccccgat gccctcggag
     2101 gcgagcctcc tgaggagatg gtagatctgg gccttggagc cgatgtcgat gccgcgggtc
     2161 ggctcgtcca tgatgacgac cttcggctcc accgagagga gtttcgatat gaggaccttc
     2221 tgctggttcc cgcccgagag gctgttggcc ttctgggcca cgttcgcgag cttgatctgc
     2281 atctcgcccg agtagcgctc ggcgagctcg gtctcggccc tggcctggat gaggccgagc
     2341 gaggcgacga gggagaggtc catgaccgag atgttcttgg cgatggagag gtcgaggaaa
     2401 aggccctcga gggcgcggtt ctcggtgagg tagacgaggc cggccccgat cgcgtcacg
//
LOCUS       DASSQV010000082         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2385845, whole genome shotgun sequence.
ACCESSION   DASSQV010000082 DASSQV010000000
VERSION     DASSQV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385845"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2016)
                     /locus_tag="VFL04_01530"
     CDS             complement(<1..2016)
                     /locus_tag="VFL04_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HET7838409.1"
                     /translation="MEAYSLGLDIGSISVNTVLLDAERRVLEDHYSLCRGRPFSALME
                     VLLDLLTRFPPGSIARVATTGTGGALAARLVGGRFVNEIVAQSDSVAALYPEARTVIE
                     IGGEDSKLIRIAPAGPAGAGPGRGPGSATRLADFAMNSVCAAGTGAFLDQQASRMGIS
                     IEEFGSLALASERPPHIAGRCSVFAKSDMIHLQQIATPTADIVAGLCFAVARNLRSNL
                     AKGRTIEAPVVFQGGVAANAGVARALREVFELGRGELLIPEHHASMGAIGAVFSLLDS
                     PPEADPSFRGLAELKAYLHDGRAPLSSWPGLARPALHATKEPRPLPATEGRVEAYLGI
                     DVGSLSTNLVLIDERTEVIARRYLATAGKPLEAIRRGLVEIEAEIGPRVEVRAVGTTG
                     SGRYLTAHFVGADVIRNEITAQATAAIHYRPEVDTVFEIGGQDSKFISIDDGVVVDFE
                     MNKVCAAGTGSFLEEQAEKLGVAIVDEFGERALSSRRPCRLGDRCTVFMESDLNSQQQ
                     KGAARDDLIGGLAYSIVQNYLQKVVGTKRVGEHILFQGGVANNPAVVAAFQAITGKPI
                     TVPPHFDVTGAIGAAMIAREELGSSGRPTRFKGFGIGTAPYAVSRFSCRGCENRCDVQ
                     EVRIEGEAGALHYGDRCDRYQAPELRQAKEQRRRAIPDLFQLRERILL"
     gene            2149..>2390
                     /locus_tag="VFL04_01535"
     CDS             2149..>2390
                     /locus_tag="VFL04_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838410.1"
                     /translation="MSDSNRTVDLVWAGISGAALFLIFILLVDAGALISLVVGAAGFF
                     RVKRKSPPTKPAAPTTREMRAPASTRRMKMRKRAAPL"
BASE COUNT          350 a          803 c          865 g          372 t
ORIGIN      
        1 gaggaggatc cgctccctca gctgaaagag gtcgggaatg gcccggcgcc tctgctcctt
       61 ggcctggcgg agctcgggag cctggtagcg gtcgcagcgg tcgccgtagt ggagggcccc
      121 cgcctccccc tcgatcctga cctcctgcac atcgcagcgg ttctcgcagc ccctgcagga
      181 gaagcggctc accgcgtagg gggcggtccc gatgccgaag cccttgaaac gcgtcggccg
      241 cccgctcgag cccagctcct cgcgggcgat catggccgcc ccgatcgccc cggtcacgtc
      301 gaagtggggg ggcacggtga tgggtttgcc ggtgatggcc tggaaggcgg cgaccaccgc
      361 ggggttgttg gccacgcccc cctggaagag gatgtgctcg ccgacgcgtt tggtccccac
      421 caccttctgg aggtagttct gcacgatgga gtaggcgagg ccgccgatga ggtcgtccct
      481 cgccgccccc ttctgctgct gggagttgag gtcgctctcc atgaagaccg tgcagcggtc
      541 gccgaggcgg cagggccgcc tcgaggacag ggcccgctcc ccgaactcgt cgacgatggc
      601 caccccgagc ttctcggcct gctcctcgag gaagctcccc gtgcccgcgg cgcagacctt
      661 gttcatctcg aagtcgacca cgacgccgtc gtcgatgctg atgaacttgg agtcctggcc
      721 gccgatctcg aagaccgtgt cgacctcggg gcggtagtgg atggccgccg tcgcctgggc
      781 ggtgatctcg ttcctgatta cgtcggcccc cacgaaatgg gcggtgaggt agcggcccga
      841 gcccgtcgtg ccgacggcgc gcacctcgac cctcgggccg atctcggcct cgatctccac
      901 gaggcccctg cggatggcct cgaggggctt gcccgccgtg gcgaggtagc gccgggcgat
      961 gacctcggtc ctctcgtcga tgaggacgag gttggtgctc aacgagccca cgtcgatccc
     1021 gaggtaggcc tcgaccctgc cctcggtggc cgggaggggc ctgggctcct tggtggcgtg
     1081 gagggcgggg cgggccagac ccggccagga cgacaaaggc gcgcggccgt catggaggta
     1141 ggccttgagc tcggcgaggc ccctgaagga ggggtctgcc tcgggcgggg aatcgaggag
     1201 gctgaagacg gccccgatcg cccccatcga ggcgtggtgc tcggggatca ggagctcgcc
     1261 ccgcccgagc tcgaagacct ccctcagcgc tcgggccact cccgcgttgg ctgctactcc
     1321 cccctggaac accacggggg cctcgatggt cctgcccttc gccaggttgc tccgcaggtt
     1381 gcgcgctacg gcgaaacaga gcccggccac gatgtcggcg gtgggcgtgg cgatctgctg
     1441 gaggtggatc atgtcgctct tggcgaagac gctgcagcgg ccggcgatgt ggggcgggcg
     1501 ctcgctcgcg agggcgaggc tcccgaactc ctcgatcgag atgcccatgc ggctcgcctg
     1561 ctggtcgagg aaggcgcccg tgcccgcggc gcagacgctg ttcatcgcga agtcggccag
     1621 ccgggtggct gagcccggtc cgcggcccgg ccctgcgccg gcaggcccgg cgggggcgat
     1681 cctgatgagc ttcgagtcct cgccgccgat ctcgatcacc gtcctcgcct cgggatagag
     1741 ggcggcgacc gagtccgact gggcgactat ctcgttgacg aagcgcccgc ccacgagcct
     1801 ggcggcgagg gctccgcccg tgcccgtcgt cgccaccctg gcgatggagc ccggagggaa
     1861 gcgcgtgagc aggtcgagga ggacctccat cagggcggag aacggcctgc cgcggcagag
     1921 gctgtagtgg tcctcgagga ccctcctctc cgcgtccagg aggacggtgt tcaccgatat
     1981 cgagccgatg tcgaggccaa gggaataggc ttccatgccc gcctccttag cagcctgacg
     2041 aggcaatagt accgaggccc cgggggcggc gcaagaagga attccgtggg ggaccttgat
     2101 gacagagctt gccgggggac gctagaattg gggcaaggag ccgggaacat gtcggactcg
     2161 aacaggaccg tggacctcgt ctgggcgggc attagcgggg ccgccctttt cctcatcttc
     2221 atcctcctcg tcgacgcggg agccctcatc tccctcgtgg tcggggccgc cggcttcttc
     2281 cgcgtgaaga ggaagagccc ccccacgaag ccggcggccc cgaccacgag ggagatgagg
     2341 gctcccgcgt cgacgaggag gatgaagatg aggaaaaggg cggccccgct
//
LOCUS       DASSQV010000083         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_768781, whole genome shotgun sequence.
ACCESSION   DASSQV010000083 DASSQV010000000
VERSION     DASSQV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768781"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VFL04_01540"
     CDS             complement(<1..953)
                     /locus_tag="VFL04_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7838411.1"
                     /translation="MIDLNVNKDQRKRNIQRCREKGIILPTYAQMRDPSKVPESVKKE
                     LSGVGLWDVHSRNLFRVTWHNEPKERGGGFGGVNYLEIPRSITGTKARIVGLVGKWFP
                     TGAHKVGAALSCLLPELVTGRFDPTTKKAVWPSTGNYCRGGAYISRLLSCPSVAILPA
                     GMSKERFEWLRTMAEEVIATPGSESNVKEIFDACVEIERTRPECVIFNQFDQLPNHLW
                     HYSVTGPPWRRPSTPSPAPATGWPARSSRPARPAPSARAPTSATASPAPRSPWPRPSS
                     APPSSKTASASTASRASGTSTSPGSTIFGIPTPPSAWTTSFR"
     gene            complement(988..>2168)
                     /locus_tag="VFL04_01545"
     CDS             complement(988..>2168)
                     /locus_tag="VFL04_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7838412.1"
                     /translation="VHGMRWSYRCPSCGAEYKIEPGRYLCDVCAPKRGKDMPLEGVLE
                     CAWELEGLEPYPQGTPLPVEREWFPKVPVGDTPLWAPERLRSELGYGRLFVKDDTCQP
                     TGSYKDRASFLVAAFARKHGIKEIALASTGNAASSMAGIGAAAGLKITVFLPKAAPAA
                     KRLQVLQYGAELRQVEGNYDQAFEESLAYSQATGVLSRNTAYNPLTIEGKKTAAFEIV
                     KDLRRSGESAGERPLEVFVPSGDGVILTGLIRGFEDLIRLGRIGSMPRFWACQAEGSS
                     ALARALAAGSFGKPVPSSTLADSIAVDVPRNGAYALSKLKKYGGRAVVVSDEEILAAQ
                     RLLSSRAGLFAEPSSSSALAGFLKAKEEIDPSSTVVLLVTGSGLKDLRSAALAVGVEL
                     "
BASE COUNT          304 a          695 c          799 g          370 t
ORIGIN      
        1 cggaagctcg tcgtccacgc cgacggcggc gtcggtatcc cgaagattgt ggatccaggg
       61 gatgtgcttg tccccgatgc cctcgatgcg gtgctcgccg aagccgtttt cgaggatggt
      121 ggggcactgg agggcctcgg ccacggcgac cttggcgccg gggaagctgt cgcggatgta
      181 ggagcccgag ccgagggtgc cggccgagcc ggacgagagg atcgcgccgg ccatcctgtc
      241 gccggggcgg gcgagggcgt ggaaggcctc ctccatggcg ggccggtgac cgagtagtgc
      301 cagaggtggt tgggcagctg gtcgaactgg ttgaagatca cgcactcggg cctggtccgc
      361 tcgatctcga cgcaggcgtc gaagatctcc ttgacgttgc tctcggagcc cggggtcgcg
      421 atgacctcct cggccatcgt gcggagccac tcgaagcgct ccttggacat gcccgcgggg
      481 aggatggcca cgctcgggca ggagaggagg cgggagatgt aggccccgcc gcggcagtag
      541 ttgccggtgc ttggccagac ggccttcttg gtcgtggggt cgaagcgccc ggtgacgagc
      601 tcgggcagga ggcaggagag ggcggcgccg accttgtggg ccccggtggg gaaccacttg
      661 ccgaccaggc ccacgatgcg cgccttcgtc ccggtgatcg agcgcgggat ctcgaggtag
      721 ttgaccccgc cgaagccgcc gcccctctcc ttgggctcgt tgtgccaggt cacgcggaag
      781 aggttgcggc tgtggacgtc ccagagcccg acgccggaga gctccttctt cacactctcg
      841 ggcaccttcg agggatcgcg catctgcgcg taggtcggga ggatgatgcc cttctccctg
      901 cagcgctgta tgttcctttt cctctggtcc ttgttcacgt tcaggtcgat catgctgttc
      961 cccttttcct tgttcgcgtt gtgcggccta gagctcgacg ccgaccgcga gcgcggccga
     1021 cctcaggtcc ttgaggccag agccggtgac gaggaggacg accgtcgacg acgggtcgat
     1081 ctcctccttc gccttgagga agccggcgag ggccgagctc gatgagggct cggcgaaaag
     1141 cccggcgcgc gacgagagca ggcgctgggc ggcgaggatc tcctcgtcgc tcacgacgac
     1201 ggcgcggccc ccgtacttct tgagctttga gagggcgtag gccccgttgc gcggcacgtc
     1261 gacggctatc gagtcggcca gggtcgagct cggcacgggc ttgccgaagg agccagcggc
     1321 cagggccctg gcgagggcgc tcgagccctc ggcctggcag gcccagaagc ggggcatgga
     1381 gccgatcctg ccgagcctga tcaggtcctc gaagccgcgg atgaggcccg tgaggatcac
     1441 cccgtcgccc gagggcacga atacttcgag gggccgctcg cccgcgctct ccccgctgcg
     1501 cctgaggtcc ttcacgatct cgaaggccgc cgtcttcttg ccctcgatgg tgagggggtt
     1561 gtaggcggtg ttgcgcgaga ggaccccggt ggcctggctg taggcgaggg actcctcgaa
     1621 ggcctggtcg tagttgccct cgacctggcg gagctcggcc ccgtactgga ggacctggag
     1681 gcgtttggcc gccggcgctg ccttgggcag gaagactgtg atcttgagcc cggccgccgc
     1741 gccgatcccc gccatcgacg aggccgcgtt gccggtcgag gccagggcta tctctttgat
     1801 cccgtgcttg cgcgcgaagg ctgccacgag gaagctcgcg cggtccttgt aggagcccgt
     1861 gggctggcag gtgtcgtcct tgacgaagag ccggccgtat cccagctcgg agcgcagcct
     1921 ctcgggcgcc cagagcgggg tgtcgccgac ggggaccttg gggaaccact cccgctcgac
     1981 ggggaggggc gtgccctggg ggtagggctc gaggccctcg agctcccagg cgcactcgag
     2041 cacgccctca agcggcatgt ccttgccgcg tttgggggcg cagacatcgc agaggtagcg
     2101 ccccggctcg atcttgtact ccgcgccgca ggatgggcag cggtagctcc atctcatacc
     2161 atggactc
//
LOCUS       DASSQV010000084         4979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2261556, whole genome shotgun sequence.
ACCESSION   DASSQV010000084 DASSQV010000000
VERSION     DASSQV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2261556"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VFL04_01550"
     CDS             complement(<1..993)
                     /locus_tag="VFL04_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HET7838413.1"
                     /translation="MKTAALIYAGSLTQRALEGLPGGDSAYKRCLDFASGLPGLERAL
                     VLEGRTTLPDGPFPRERREYWTIDGVLERLGGLGEGLDAVFYFWADQPFLDAKLAARM
                     LENFRRYRAQYGFADGYPVGLSTEILSPRIVPALRSLAAGSDGGGGAAPARDSLFAVI
                     QKDINAFDIETEIAPLDLRDLRLTLACDSKRETLLVERLMAAGGSDEPSLLELIPRRL
                     DLLRSLPAFVQVQVSGACPQTCQLCPYPKFGGDILSRSDFMPRNAFADILDQVVDLSD
                     DAVIDISLWGEPSLHPDIAGLIDEVLARPSLSLIVETSGIGWDRSLPERIAGHQG"
     gene            1297..4941
                     /locus_tag="VFL04_01555"
     CDS             1297..4941
                     /locus_tag="VFL04_01555"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HET7838414.1"
                     /translation="MKFERHYTKKDSGPYRDIKWEKRLSEIRNPDGKSVFRMEGAVVP
                     STWSQIATDIIAQKYFRKAGVPADRARAWEAFVPKSQLVLATDSPLAGAEHDSRQVFH
                     RLAHTWREWGKTAGYFDTEEDASAFYDELLYMLAHQVAAPNSPQWFNTGLHAVYGIEG
                     PAQGHFYVDPATGEVTKSSSAYARPQPHACFILDIEDDLVNEGGIMDLVTREARLFKY
                     GSGTGSNFSKIRGLNEPLSGGGVSSGLLSFLKIADRSASAVKSGGTTRRAAKMVILDA
                     DHPDIEQYSKWKAEEEYKVACLAAGSALVRSHAQALREAVGSDSAMALPPDERFSFER
                     NAELRKAAKLALRSGVPAPFLHQLLSMYRQGDFAAELPLYDTAWEGEAYNTVSGQSSN
                     NSVRVDNRFMKAVQEDGDWDLIGRITGKTVRKTKARSVWDTIAKAAWKCADPGLQFHG
                     TINEWHTCPADGEIRGSNPCSEYMFLDDTACNLASLNLMAFYDEKTGRFDEVAYRHAV
                     RLWTTVLEISVVMAQFPSKEIARRSYDYRTLGLGYANLGSLLMVMGLAYDSGEGRSVA
                     AALSAILTGEGYAQSARMAEAWGPFARYEANKEHMLRVIRNHRRAAMGAGRDEYEGLT
                     VTPEPLDPKLCPANLAKAARESWDEALKIGEKQGFRNAQVSAIAPTGTIGLLMDCDTT
                     GVEPDFALVKFKKLAGGGYFKIINQSVPPALRALGYDEKSILEIEAYALGHGTLKGAG
                     AKAPGAAAAGPGADRPCLSYEGLLALGLPEASLARVEAALATAISLDSCFAPHVIGKD
                     GFAAIGLPESTWSLPGFSLLRHLGFSEEEIERAELFACGTMGLEGAPGLKTEHLPVFD
                     TATPSGKRGTRSIQWRAHISMMAAVQPFISGAISKTINMPNSASYEDVKGAYSQAWHS
                     MLKAIALYRDGSKLSQPLSALAPGADIIADSILALQTGELDDEQAEEGLGAAGGPGTR
                     VEAELPGMPAAPRGVRRSLPNRRSGYTQKAKIGGHSVFLRTGEYEDGNLGEIFLDMHK
                     EGAAFRSILNSFAITVSLGLQYGVPLEEYVDAFTFTRFEPNGLVQGHDYVKMSTSVLD
                     YIFRDLAIAYLSRHDLGQVKPEDLETTSTQRKPSFGQAGFKTAAARGGSASGLGAAAP
                     SPAPGAAGGLTGALSGIAAQIKAAREGSRNARLKGYEGDPCPVCGHLTLVRNGTCLKC
                     ETCGGTTGCS"
BASE COUNT          862 a         1749 c         1632 g          736 t
ORIGIN      
        1 cccctggtgg ccggcgatcc gctcgggcag gctccggtcc cagccgatgc ccgaggtctc
       61 cacgatgagg ctcaggctcg gccgggccag gacctcgtcg atgaggccag cgatgtcggg
      121 gtggaggctg ggctcacccc agagcgagat gtcgatgacg gcgtcgtctg agaggtctac
      181 gacctggtcg aggatgtcgg cgaaggcatt ccggggcatg aagtccgagc gcgagaggat
      241 gtcgccgccg aacttggggt aggggcagag ctggcaggtc tgcgggcagg cgccggagac
      301 ctggacctga acgaaggccg gcaggctccg cagaaggtcg agacggcggg ggatgagctc
      361 gagcaggctc ggctcgtccg agcccccggc cgccatgagc cgctccacaa gcagggtctc
      421 gcgcttggag tcgcaggcca gggtcaggcg gaggtccctg aggtcgaggg gggcgatctc
      481 ggtctctatg tcgaaggcgt tgatgtcctt ctggatgacc gcgaaaagcg agtccctggc
      541 cggggcggcg ccacccccgc cgtcggaccc cgccgcgagc gaacgcaggg ccgggactat
      601 cctcggcgaa aggatctcgg tcgacagccc gaccgggtag ccgtcggcga agccgtactg
      661 ggcccggtag cgccggaaat tctcgagcat cctcgcggcg agcttggcgt cgaggaaggg
      721 ttggtcggcc cagaaataga agacggcgtc gaggccctcg ccgaggccac cgagcctctc
      781 aaggactccg tcgatcgtcc agtactcccg ccgttcgcgg gggaaggggc catcgggcag
      841 ggtcgtccgg ccctcgagga caagggcccg ctcgagcccc ggcaggcccg aggcgaaatc
      901 gagacagcgc ttgtaggctg agtcgccccc tgggaggcct tccagagccc gctgggtgag
      961 ggagccggca tagatgagtg ctgcagtttt catggtcagt ctcgattatc ggccggcctt
     1021 ggcctgcatt cgcaggccat tttccacggc gagccacgat ttcggcccgg accaagccca
     1081 agggaagcgg cggtcgttca tccctcaagc ctcccttctg aacttcgatt caggatacac
     1141 ctcggtctca agctgtcaag gccagggaaa aagcgatata ttctacctgt agggtattgt
     1201 ccccgccggc accaccatat atagtagccg tcgagtgcag tgccattcta tcataaatgg
     1261 cccgcggcgt ttaaggcggc gcggggaggg gttcatatga aattcgagcg ccattacacg
     1321 aagaaggatt cgggcccgta ccgcgacata aagtgggaga agaggctcag cgagatacgc
     1381 aaccccgacg ggaagtcggt cttcaggatg gaaggggccg tggtcccctc gacctggagc
     1441 cagatcgcca ccgacatcat cgcccagaag tacttcagga aggcgggggt acccgcagac
     1501 agggcgaggg cctgggaggc cttcgtgccc aagtcgcagc tcgttttggc caccgactcc
     1561 cccctggcag gggccgagca cgactcccgc caggtcttcc accgcctggc ccacacctgg
     1621 agggagtggg gcaagaccgc cggctatttc gacaccgagg aggacgcctc cgccttctac
     1681 gacgagctcc tctacatgct cgcccatcag gtcgccgccc cgaacagccc ccagtggttc
     1741 aacaccgggc tccacgccgt gtacggcatc gagggcccgg cccaggggca cttctacgtc
     1801 gacccggcga cgggcgaggt taccaagtcc tcctcggcct acgccaggcc ccagccccac
     1861 gcctgcttca tcctcgacat cgaggacgac ctcgtgaacg agggcgggat catggacctc
     1921 gtcacgcgcg aggcgaggct cttcaagtac ggttcgggca cgggctcgaa cttctccaag
     1981 atcaggggcc tgaacgagcc cctctcgggc ggcggggtct cgagcggcct cctgtctttc
     2041 ctcaagatcg ccgaccgatc ggccagcgcc gtcaagtcgg gggggaccac gcgccgcgcg
     2101 gcgaagatgg tgatcctcga cgccgaccat cccgacatcg agcagtactc gaaatggaag
     2161 gccgaggagg aatataaggt ggcctgcctc gccgcaggct cggccctcgt gcggagccat
     2221 gcccaggccc tccgcgaggc tgtgggctcg gactcggcca tggcccttcc gcctgatgag
     2281 cgcttctcct tcgagcgcaa cgccgagctc aggaaggcag ccaagctggc cctcaggtcg
     2341 ggggtcccgg cccccttcct ccaccagctc ctctccatgt accgccaggg cgacttcgcc
     2401 gccgagctcc ccctctatga cacggcctgg gagggcgaag cctacaacac ggtctcgggc
     2461 cagtcctcaa acaactccgt gcgggtcgac aaccgcttca tgaaggccgt ccaggaggac
     2521 ggggactggg acctcatcgg acgcatcacc ggcaagactg tcaggaagac caaggcccgc
     2581 tcggtctggg acacgatcgc gaaggctgcc tggaagtgcg ccgaccccgg gctccagttc
     2641 cacggcacca tcaacgagtg gcacacctgc ccggccgacg gcgagatccg cggctcgaac
     2701 ccctgctccg agtacatgtt cctcgacgac accgcctgca atcttgcctc gctcaacctc
     2761 atggccttct acgacgagaa gacggggcgc ttcgacgagg tggcctaccg ccacgccgtc
     2821 aggctctgga cgaccgtcct cgagatctcg gtcgtcatgg cccagttccc ctcgaaggag
     2881 atcgcgagga ggagctacga ctacaggacc ctgggcctgg gctacgccaa cctcggcagc
     2941 ctcctcatgg tcatgggtct cgcctacgac tcgggcgagg ggcgctcggt cgcggccgcc
     3001 ctctcggcga tcctcaccgg cgagggctac gcccaatcgg cgcggatggc cgaggcctgg
     3061 ggacccttcg cccggtatga ggccaacaag gagcacatgc tcagggtcat caggaaccac
     3121 cgccgcgccg cgatgggcgc ggggagggat gagtatgagg gcctcaccgt gacgcccgag
     3181 cccctcgacc cgaagctctg ccccgcgaac ctggccaagg ccgccaggga gtcctgggac
     3241 gaggccttga agatcgggga gaagcagggc ttccgcaacg cccaggtgag cgccatagcg
     3301 cccacaggca cgatcggcct cctcatggac tgcgacacca cgggcgtcga gcccgacttc
     3361 gccctcgtga agttcaagaa gctcgccggc ggcggctact tcaagatcat caaccagagc
     3421 gttcccccgg ccctgcgcgc cctcggctac gacgagaaga gcatcctcga gatcgaggcc
     3481 tacgccctcg gccacgggac cctgaagggg gccggagcca aggcgcccgg cgccgcagcc
     3541 gctggccccg gggccgaccg gccctgcctc tcctacgagg gcctcctcgc cctcggcctg
     3601 cccgaggcct ccttggcgag ggtggaggcc gccctcgcga ccgccatctc gctcgactcc
     3661 tgtttcgccc cccacgtcat cgggaaggat ggcttcgccg ccatcggcct gcccgaatcc
     3721 acctggtccc tcccgggctt cagcctcctt cggcacctcg gcttctccga ggaggagatc
     3781 gagagggccg agctcttcgc ctgcggcacg atgggcctcg agggggcgcc cggcctcaag
     3841 accgagcacc tgccggtctt cgacacggcg accccctcgg gcaagagggg cacgcgctcg
     3901 atccagtggc gggcccacat ctccatgatg gccgccgtcc agcctttcat ctcgggcgcg
     3961 atctccaaga ccatcaacat gcccaactcg gcgagctacg aggacgtcaa gggcgcctac
     4021 tcccaggcct ggcactccat gctcaaggcc atcgccctct accgcgacgg ctccaagctc
     4081 tcccagcccc tctcggccct ggcgcctggg gccgacatca tcgcggattc gatcctggcc
     4141 ctccagacgg gggagctcga cgatgagcag gccgaggagg ggctcggcgc agcgggaggg
     4201 cccggcacgc gggtcgaggc cgagctgccg ggcatgccgg cggccccgcg cggggtccgc
     4261 aggtccctgc cgaaccgccg ctcgggctac acccagaagg ccaagatcgg gggccactcg
     4321 gtcttcctcc gcacagggga atacgaggac gggaacctcg gcgagatctt cctcgacatg
     4381 cacaaggagg gagcggcctt ccgctcgatc ctgaactcct tcgccatcac cgtgtccctc
     4441 ggcctccagt acggcgtccc cctcgaggag tatgtcgacg ccttcacctt cacccgcttc
     4501 gagccgaacg gcctcgtcca gggccacgac tacgtcaaga tgtcgacgtc ggtgctcgac
     4561 tacatcttcc gcgacctcgc gatcgcctac ctgagccgcc acgacctggg ccaggtcaag
     4621 cccgaggacc tcgagaccac ctcgacccag aggaagccct ccttcggcca ggcaggcttc
     4681 aagaccgcgg ccgcgagggg gggctcggcc tcgggccttg gcgccgcggc cccgagcccc
     4741 gccccgggcg ctgcgggcgg cctgacgggc gccctctcgg gcatcgccgc gcagatcaag
     4801 gcggccaggg agggctcgcg caacgcccgg ctgaagggct acgagggcga cccctgcccc
     4861 gtctgcggcc acctcaccct cgtgcgcaac gggacatgcc tcaagtgcga gacctgcggt
     4921 ggcaccacag gatgcagctg aggcaggtct cgcacttgag ggtgtcactc agcgcggaa
//
LOCUS       DASSQV010000085         4980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_23269, whole genome shotgun sequence.
ACCESSION   DASSQV010000085 DASSQV010000000
VERSION     DASSQV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23269"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFL04_01560"
     CDS             complement(<1..446)
                     /locus_tag="VFL04_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838415.1"
                     /translation="MPVFLILGALVIALCAIVLSIAMRGTKRYSWLEFQLKTHEAGLS
                     FAEARSLSEAASAAGIADTTNVLWSPRDLDRAIVVLLGRLRDEGRDRTREGVALMDRV
                     YALRKRLEFELPRYKYGIRSSRNIANGQRVRILVEGLGVMNSTIID"
     gene            543..1223
                     /locus_tag="VFL04_01565"
     CDS             543..1223
                     /locus_tag="VFL04_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7838416.1"
                     /translation="MDEAETRTAPARPLLEAGLVFAALYLSAYLPADPRAIADSLGKP
                     GFHVSILAALVPKALLVLYLIARSDGFAAFGITRPRLADLAHALLCALGAVAVLVLPS
                     ALLLSFGLRNPLMEATRAPGGPSLLLVPLVLASSLAVGYGEEIFFRSYLIRRLRQAGL
                     SAPWAALASSLVFGSAHGVQGPVGLALASLLGLWFAWRWLDGGRIHEIAVGHAMYDAG
                     VILFALFR"
     gene            1256..1618
                     /locus_tag="VFL04_01570"
     CDS             1256..1618
                     /locus_tag="VFL04_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838417.1"
                     /translation="MQPTDKFRTDIDQALRRGGADDRARLESLRSFFYRSFPGVSEEA
                     KASLFAAFLEKWRAEPEGAKAWLAGAGSVLFMDYDGTPFSREDWAELREAVSIDAERI
                     DMDLLEYVMTLVLDHGAL"
     gene            1615..2421
                     /locus_tag="VFL04_01575"
     CDS             1615..2421
                     /locus_tag="VFL04_01575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7838418.1"
                     /translation="MKSGEEWFEDESFWTAYAPLMFDEGRWAEVPAVIDSIEALAKPV
                     AGGAVLDACCGPGRHSLELASRGYRVTGIDITRAYLEAARESAAAWAVDAEFLRADLR
                     EFVRPGAFDLALNLYTSFGYFADPGDDLTALQNLHDCLAPGGALVLETLGKEVAAKDF
                     VEGEWFERGEWMVLTQYEVVGAWEGLRNRWIMIPRELAAPPSEGRPAANEDPRGHAAA
                     DRSFVLRLYSGSELRDALFASGFRQVEIYGSLAGGPYDQTAKSLVAVARS"
     gene            2504..3478
                     /locus_tag="VFL04_01580"
     CDS             2504..3478
                     /locus_tag="VFL04_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET7838419.1"
                     /translation="MSENIGLKLANGEFYPVLQADTAARKRLVVTTVQDSQASVQIDL
                     YRGSGPRTEGASYIGSLVIEGIPPRTKGEPDIRMDLGLDADGTLTAYAVETSTGASQS
                     LKVSLESLAEEEKYEIPDFEFSRYSEAEVEAKPEPSFADEDFAETDVLPGEEANVRLL
                     ADEAPPEEPAPRRSKTWLVVLAIVAALALVLVLAYFLIPGLGAARPEPAANAARSAQV
                     APVQPAPSQAPAASAARPAAAAPVTAAAAPEAAPAAAPQAPIVSEKPGTPAKQPGAYY
                     RIKWGDTLWDLSYSFYRNPWLFGKIAKANKIRNPDLIFSGNKIWIPPR"
     gene            3500..>4980
                     /locus_tag="VFL04_01585"
     CDS             3500..>4980
                     /locus_tag="VFL04_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838420.1"
                     /translation="MRPEPRVSSTLALILAFSPLILASCQERQAFGLGEKRLSEILAA
                     KDPGPILELDPKSLGDPGFYGPPGYYYMARWIEARTPRASAQKTTAPHAADTGTAEKL
                     AEGGVPQPAARSNPADDVRLLLRLAYDRGEGLVRMEAGRLLALRLAEGGKWDELLALA
                     EDYRKTIGPDWSVERSRLEALEGLGEDAELIQSLSGLRADFPSETSADADALLYFEGV
                     AAERLDRPGWPAPFRQLLLERPASAWTGKALDFVSSLEKHPKEFSLDERRAARMRTLV
                     RDRDYGPGYEAAMQARDFVLSPLRSPSLIADAAKAFLYSGSSKEGLVRFARLESEAMQ
                     KVDLAPDPASSARAARVAWTAAYYRARFYRALELWPDAAEGFRHAVDLAPAPADVDDS
                     LWYQVECQEKLAIQKAKKAKPGSAAALRTEALVRRRQLEALEAASRIWADPSKFADLV
                     DELTRDALRARDWYIVDHLSAGLAREVSPSMGARCAYVATRAE"
BASE COUNT          773 a         1819 c         1604 g          784 t
ORIGIN      
        1 ttgtcgatga tcgtggagtt catgaccccg aggccttcga ccaggatgcg gaccctctgc
       61 ccgttggcga tgttcctgct cgacctgatg ccgtacttgt agcggggaag ctcgaactcg
      121 agccgtttgc ggagcgcgta gacccggtcc atgagggcca cgccctccct ggtgcggtcc
      181 cggccctcgt ccctgagcct gccgagcagg accacgatcg cgcggtccag gtccctgggg
      241 gaccagagca cgttggtggt gtccgcgatg cccgcggccg aggcggcctc gctgagggac
      301 ctcgcctcgg cgaacgagag ccccgcctca tgggtcttga gctggaattc gagccaggaa
      361 tagcgcttcg tgccgcgcat cgcgatgctg aggacgatgg cgcacagggc gatgacaagc
      421 gctccaagaa tgaggaatac tggcatgcgg ttcggctctc tcgtttccgg gccgcgtcgc
      481 gacccgcctt gagtatacta tacaaccatc aaacttggta ttcagttcgg aggccgggac
      541 ttatggacga agcagagacg aggacggccc cggccaggcc cctcctcgag gcgggactgg
      601 tcttcgccgc gctctacctt tcggcctacc tccccgccga tccgcgggcc atcgccgatt
      661 ccctcggcaa gcccggtttc cacgtctcga tcctggctgc cctcgtcccg aaggccctcc
      721 tcgtcctcta cctcatcgcc cgctcggacg ggttcgccgc cttcggcatc acgaggccaa
      781 ggctggccga cctcgcccac gccctcctct gcgccctggg cgccgtcgcc gtcctcgtgc
      841 ttccctcggc cctcctgcta tccttcgggc tgaggaatcc cctgatggag gccactcggg
      901 cccccggggg gccctcactc ctcctcgtgc cccttgtgct cgcctcatcc ctcgccgtcg
      961 gctatgggga ggagatcttc ttcaggtcct acctcatccg ccggctacgc caggccgggc
     1021 tctcggcccc atgggccgcc ctcgcctcga gcctcgtctt cggcagcgcc cacggcgtcc
     1081 agggccctgt cgggctcgcc ctggcgagcc tcctcgggct gtggttcgcc tggcgctggc
     1141 tcgacggcgg gaggatccac gagatcgcgg tcgggcatgc gatgtacgat gccggggtca
     1201 tccttttcgc cctcttccgc tgacgcgatc cgcgccgaag gctatactct atcccatgca
     1261 gccgacagac aagttcagga ccgacatcga ccaggccctg cgccggggcg gcgccgacga
     1321 cagggcccgt ctcgaatccc tccgctcctt cttctaccgc tccttccccg gcgtgagcga
     1381 ggaggcgaag gcatccctct tcgccgcctt cctcgagaag tggcgcgccg agcccgaggg
     1441 agccaaggcc tggctcgccg gcgcggggag cgtcctcttc atggactacg acggcacccc
     1501 cttctcccgc gaggattggg ccgagctccg cgaggccgtc tccatcgacg ccgagaggat
     1561 cgacatggac ctcctcgaat acgtcatgac cctcgtcctc gaccacggag ccctatgaaa
     1621 agcggcgagg agtggttcga ggacgagtcc ttctggacgg cctacgcccc gctcatgttc
     1681 gacgagggcc gctgggccga ggtgcccgcg gtcatcgact cgatcgaggc cctcgccaaa
     1741 cccgtcgccg ggggcgccgt cctcgacgcc tgctgcggcc cggggaggca cagcctcgag
     1801 ctcgcctcga ggggctacag ggtcacgggc atcgacatca cccgggccta cctcgaggcc
     1861 gcgcgcgaaa gcgccgcggc atgggccgtc gatgccgagt tcctgagggc ggacctcagg
     1921 gagttcgtcc gcccgggggc cttcgacctg gcactcaacc tgtacaccag cttcggctat
     1981 ttcgccgatc ccggcgacga cctcacggcc ctccagaacc tccacgattg cctcgcgccg
     2041 ggaggggccc tcgtgctcga gaccctcggc aaggaggtcg ccgccaagga cttcgtcgag
     2101 ggggagtggt tcgagcgggg cgaatggatg gtcctcacgc agtacgaggt ggtgggggcc
     2161 tgggaggggc tcaggaaccg ctggatcatg atcccgcggg agctcgccgc gccgccatcc
     2221 gaggggaggc cggcggcgaa tgaggatccg cggggccacg cggccgccga ccggagcttc
     2281 gtcctccgac tctactcggg cagcgagctc cgcgatgccc tcttcgcctc gggcttccgc
     2341 caggtcgaga tatacgggag cctcgcgggc gggccctacg accagacggc aaaaagcctt
     2401 gtggcggttg cgaggagttg atatctccgc catcggctgt acaattaagc ctatacgcgg
     2461 cccatagagg gatgcgcgac atcaccgccg accggaaggc gccatgagtg aaaacatcgg
     2521 cctgaagctc gcgaacggcg agttctaccc cgtcctccag gcggacacag ccgcccgcaa
     2581 gcgcctcgtc gtgacgaccg tccaggacag tcaggcgagc gtacagatcg acctctaccg
     2641 gggctcgggc ccaaggaccg agggcgcgag ctacatcggg agccttgtca tagagggcat
     2701 cccgccgcgc acgaagggcg agcccgacat caggatggac ctcggcctcg acgccgatgg
     2761 gaccctgacg gcctacgccg tggagacctc caccggcgcg agccagagcc ttaaggtctc
     2821 tctggaatcc cttgccgagg aagagaaata cgagatcccc gatttcgagt tcagcaggta
     2881 cagcgaggct gaggtcgagg ccaagcccga gccgagcttc gccgacgagg acttcgccga
     2941 gaccgacgtc ctccccggcg aggaggccaa cgtcaggctc cttgccgacg aagcccctcc
     3001 cgaggagccc gcgccgcggc gttccaagac ctggctcgtg gtgttggcca tcgtggcggc
     3061 cctggcgctt gtcctcgtcc tcgcatattt cctcatccct ggcctcggcg ccgcgcggcc
     3121 cgagccggcc gcgaacgcgg ccaggagcgc ccaagtggct cccgttcagc ccgcccccag
     3181 ccaggcgccc gccgcctctg cagccagacc tgcggccgcg gctcccgtca cggccgccgc
     3241 ggccccggaa gccgcccccg ccgctgctcc gcaggccccg atcgtgtcgg agaagccagg
     3301 gaccccggcc aagcagcctg gtgcctacta tcggatcaag tggggggaca cgctctggga
     3361 tctctcgtat tccttctacc ggaacccctg gcttttcggg aagatcgcca aggccaacaa
     3421 gatcaggaac cccgacctca tcttctcggg caacaagatc tggataccgc cgcgataaaa
     3481 tgctaggctc ggtgttccca tgagacccga gccccgcgtc agctccacac tcgccctcat
     3541 cctcgccttt tcccccctca tcctggcctc ctgccaggag aggcaggcct tcggtctggg
     3601 cgagaagcgc ctttcggaga tcctcgcggc caaggacccg ggccccatcc tcgaactcga
     3661 ccccaagagc ctcggcgatc ccggcttcta cggccctccc ggttactact acatggcccg
     3721 gtggatcgag gcgaggacgc ccagggcctc ggcccagaag accacggctc cgcacgccgc
     3781 cgacacgggc acggcggaga agctcgccga gggcggggtc ccccagcccg ccgcacggtc
     3841 caacccggcg gacgacgtca ggctcctcct ccggctggcc tacgaccgcg gcgagggcct
     3901 tgtcaggatg gaggcgggca gactcctcgc cctccgcctc gccgaggggg ggaaatggga
     3961 tgagctcctc gccctcgcgg aggactacag gaagacgatc ggccccgact ggtcggtcga
     4021 gcgctcaagg ctcgaggccc tcgagggcct cggcgaggac gccgagctca tccagagcct
     4081 gagcgggctg agggccgact tcccgtccga gacgagcgcc gatgccgacg ccctccttta
     4141 cttcgagggc gtggcggccg aacgtctgga ccggcccggc tggcccgcgc ccttccgtca
     4201 gctcctcctc gagaggccgg cttcggcatg gacgggcaag gccctcgatt tcgtctcctc
     4261 cctcgagaag catccgaagg aattctccct cgatgagcgc agggccgcgc ggatgcgcac
     4321 cctggtccgg gaccgcgact acgggcccgg ctacgaggcg gcgatgcagg cgagggactt
     4381 cgtcctctcg cccctgcgct cgccgagcct gatagccgac gccgccaagg ccttcctcta
     4441 ttcgggctcc tccaaggagg gcctggtccg tttcgcccgc ctcgagtccg aggccatgca
     4501 gaaggtggac ctggctcccg accccgccag ctcggcccgc gccgcaaggg tggcctggac
     4561 cgcggcctac tatcgcgccc gcttctatcg cgccctcgag ctctggcccg acgccgcgga
     4621 gggcttcaga cacgctgtcg acctcgcccc ggcacccgcc gacgtcgacg attcgctctg
     4681 gtaccaggtg gagtgccagg agaagctcgc catccagaag gcgaagaagg ccaagcccgg
     4741 cagcgccgcc gcgctgcgga ccgaggccct cgtgcgaagg cgccagctcg aggccctcga
     4801 ggccgcatcc aggatctggg ccgacccatc gaagtttgcc gacctcgtcg acgagctcac
     4861 aagggatgcg ctccgagccc gggactggta catcgtcgac cacctttcgg cgggcctcgc
     4921 gcgcgaggtt tcgccttcga tgggagcgag gtgcgcctat gtcgcgacca gggcggagga
//
LOCUS       DASSQV010000086         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_645148, whole genome shotgun sequence.
ACCESSION   DASSQV010000086 DASSQV010000000
VERSION     DASSQV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645148"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VFL04_01590"
     CDS             <1..997
                     /locus_tag="VFL04_01590"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET7838421.1"
                     /translation="VGPGDLVATSPYTFTSSAAVARHLGAEVRFCDISEADYNIDPEA
                     LEDLLKREAGRIKALVLVHIGGLPCRVAEILAIARRHGCAVVEDAAHAFPVRLSEGTV
                     GTLGDIGVYSFYATKTITTGEGGMVVTADEGLRKRMALMRLHGFDREAWDRYTSKQAS
                     WLYGVAEAGYKYNLPDILAAIGREQLKKAERFLAERKAIAERYLEAFSGIGAVECPPL
                     HPSHSWHLFSLRVRAEGLGFGRDEFIERLRAKGIGTSVHFIPLHLMRYWAERYSLKPS
                     DFPRALEKYQRSLSLPIWHGMSEAQVEKVAEAVLEVCGASGARRAGSAASTARGA"
     gene            1039..>2438
                     /locus_tag="VFL04_01595"
     CDS             1039..>2438
                     /locus_tag="VFL04_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HET7838422.1"
                     /translation="MTDFQAEGMIHATLVRSQIAKGEIRSIVFPRLPPGYRTILPADI
                     PGDNRIVSFGAEIPILAEERISYAGEPVALIAGPDPILVDELAAATRVMCAEDEPYLA
                     WESFSSDQVAAKRVVVVGDPDLALSISGAVYEGTYRSGSIAHFYSEPQGAVAAYDYNK
                     MAIWCATQWPYHVRDSVARALACRHEDVSVRPTRLGVHLDGKLWYPSFLATHAAVAAV
                     VCGRPVKILLTREEDHRFAPKRARSSVSVRAALNPGGDLAALDVRIAINVGAYSPLAE
                     EILSQAVIAATGAYSCPNLRIEGYAVATNTPPLGAFGGLGASHTFFAIEAHANRIAAA
                     LGDDPLDWKSRNVLRKGSRLLTGEVLDEEVPYAAIAERLVAMSDYRRKWAGYELVRKR
                     RSARESGSLRGIGLAFAYQGAGPFLSGGASNSYALEATLDKDLKVWIRTSAAVSGEGV
                     IDIWRRSASALLQVPM"
BASE COUNT          380 a          924 c          776 g          358 t
ORIGIN      
        1 cgtggggccc ggagacctcg tcgcgacgag cccctacaca ttcacctcga gcgcggcagt
       61 ggcccgccac ctcggcgccg aagtccgctt ctgcgacatc tccgaggccg actacaacat
      121 cgatcccgag gccctcgagg acctgctcaa aagggaggcg gggcggatca aggccctcgt
      181 cctcgtccac atcggcggcc tcccctgcag ggtggccgag atcctcgcca tcgccaggcg
      241 ccacggctgc gccgtggtcg aggacgccgc ccacgccttc cccgtgcgcc tgtccgaggg
      301 tacggtcggg accttgggcg acatcggcgt ctactccttc tatgccacca agacgatcac
      361 gaccggagag ggcgggatgg tggtcaccgc cgacgagggc ctgcgtaagc gcatggccct
      421 catgcggctc cacggcttcg accgcgaggc atgggaccgc tatacctcga agcaggcctc
      481 ctggctgtac ggggtcgccg aggccggcta caagtacaac cttcccgaca tcctcgcggc
      541 gataggcagg gagcagctca agaaggccga gcgtttcctc gccgagcgga aggcgattgc
      601 cgaacgctac ctcgaggcct tctccggcat tggggccgtc gaatgccccc ccctccatcc
      661 ctcccactcc tggcacctct tcagcctcag ggtcagggcc gagggcctgg gctttggccg
      721 cgacgagttc atcgagcgcc tcagggccaa gggcatcggg acctcggtgc acttcatccc
      781 actccacctc atgcggtact gggccgagcg ctacagcctc aagcccagcg acttcccccg
      841 ggccctcgag aagtaccagc gcagcctttc cctgcccatc tggcacggca tgagcgaggc
      901 ccaggttgag aaggtggccg aggccgtcct ggaggtctgt ggggcctctg gcgcgcggcg
      961 ggccggctcg gccgcgtcca cggcaagggg cgcctgacgc cgcggcgacg cgaagccgag
     1021 ctcgagcgcc gtctctcggt gaccgatttc caggccgagg gcatgatcca cgcgacactc
     1081 gtccgctcgc agatcgccaa gggcgagatc cgctcgatcg tcttcccccg cctgccgccc
     1141 ggataccgga ccatcctccc cgccgacata ccgggggaca accggatcgt gtccttcggg
     1201 gccgagatac ccatcctcgc cgaggagcgg atctcctacg ccggagagcc cgtcgccctg
     1261 atcgccggac cagacccgat cctcgtcgac gagctcgccg ccgcgacgag ggtcatgtgc
     1321 gccgaggacg agccctacct cgcctgggag agcttctcct ccgaccaggt cgccgccaag
     1381 cgggtcgtgg tcgtggggga ccccgacctc gccctctcga tctcaggcgc ggtctacgag
     1441 gggacctaca ggagcggctc gattgcccac ttctacagcg agccccaggg agccgtcgcc
     1501 gcctacgact acaacaagat ggcgatctgg tgcgcgaccc agtggcccta tcatgtccgc
     1561 gattcggtcg cgcgggccct cgcctgcaga cacgaggacg tctcggtccg ccccacccgc
     1621 ctcggcgtcc acctcgacgg caagctctgg tacccctcct tcctcgccac ccacgccgcg
     1681 gtggcggccg tcgtctgcgg ccggcccgtg aagatcctcc tcacccggga ggaggaccac
     1741 cgtttcgccc ccaagcgggc gcggagctcg gtcagcgtga gggccgccct caaccccggc
     1801 ggcgacctcg cggccctcga cgtgaggata gccatcaacg tcggcgccta ctcccccctc
     1861 gccgaggaga tcctgagcca ggccgtcatc gccgcgaccg gggcctattc ctgccccaac
     1921 ctcaggatcg agggctacgc cgtggccacg aacacccccc ccttgggcgc ctttgggggc
     1981 ctcggggcct cccacacctt cttcgccatc gaggcccacg ccaacaggat agcggccgcc
     2041 ctcggcgacg accccctcga ctggaaatcg cgcaacgtgc tgcggaaggg ctccaggctg
     2101 ctgacgggcg aggtcctcga cgaggaggtg ccctacgcag ccatcgccga gaggctcgtg
     2161 gcgatgagcg actaccgccg caagtgggct ggctacgagc tcgtccgcaa gcggcgctcg
     2221 gccagggaga gcgggagcct ccgcgggatc ggcctcgcct tcgcctatca gggcgccggc
     2281 cccttcctct cagggggggc ctccaactcc tacgccctcg aggcgaccct cgacaaggac
     2341 ctcaaggtct ggatcaggac cagcgccgcc gtgagcggcg agggcgtcat cgacatctgg
     2401 aggcgctcgg cctcggccct cctccaggtc cccatgga
//
LOCUS       DASSQV010000087         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2013371, whole genome shotgun sequence.
ACCESSION   DASSQV010000087 DASSQV010000000
VERSION     DASSQV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013371"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFL04_01600"
     CDS             complement(<1..306)
                     /locus_tag="VFL04_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838423.1"
                     /translation="MASRRPSDRQGAVSRLAILIAISAILSSCGSPRDAFIVGTKQER
                     SELRELFGILDSAGQDPVQRFAAVRQISADLLAHREYGRLTALLTGLAARAAPASETP
                     "
     gene            375..1010
                     /locus_tag="VFL04_01605"
     CDS             375..1010
                     /locus_tag="VFL04_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838424.1"
                     /translation="MKRSRSRPDDVLFIAALAAVLLGIELLLYTTGAYTGALQVWPFS
                     ALVAGGLLLYLVFVRGFSHYFFFGGILFELVGLIFIAGGLSGWTLREAWPLGMVAAGL
                     AGIAGGIFRWRRFRARYAAPSAGFVVLGSVFALFSFRAITIGFGRFVAEWWPSLLIAG
                     GVFLFGAYGFSARGDSSVRGQRPERGSGKQAGGERRSGRTRRPSGREGPQG"
     gene            1034..>2495
                     /locus_tag="VFL04_01610"
     CDS             1034..>2495
                     /locus_tag="VFL04_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838425.1"
                     /translation="MLASVLVAALLADSCASHQAGPPDPAGSPVPTPGPQADAEPPQA
                     GPGTAIQISAAPKASRAQGDRAAIEEAIVLGSPASLQRARSLLSSSSNLKPEESSALG
                     LAINAFDALIYGSADAATLLAVGVPPGMGGVSALIAKTAVDAFAGRVDDAPPDYGASG
                     LAELLPALAMFASPSRDTARQAQDALERFSGLGYASVIPDLALGLDAERRLDWKAALA
                     RYTEALDLAEDAWPARLGVARSELALGQAVEALAILGPLADRLSGSSAFIRTYAEALY
                     ENARFAQADQYVARVLVDDPQNSRFILIRAHILVRSGSYQQAVPLLDAYATVDSSNRL
                     FLLLRSQVAEGMRDRDDALRWARRGLAAYADDPELLVTAARLRFSGPSAGRQEARSLA
                     ARAYELTPPAQGSEGFAPVQLEARNAAAFDAARLLAADAAARFEWLPAAQYLERATSY
                     GSFEDRALASTILRRSGRAAAALEYADSWYRDRPESE"
BASE COUNT          327 a          931 c          822 g          415 t
ORIGIN      
        1 cggggtctcg gaagccggag ccgcgcgggc cgcgaggccg gtgaggaggg cggtgaggcg
       61 tccgtactcg cggtgggcga ggaggtcggc cgagatctgc cgcacggcgg cgaagcgctg
      121 gacgggatcc tggccagccg aatcaaggat gccgaagagt tcgcgcaact cggagcgctc
      181 ctgtttcgtg cccacgatga aggcgtcgcg cggtgagccg caggaggaga ggatggcgct
      241 tatggctatc aggatggcga ggcgcgagac agcgccctgt ctgtcggaag gccgccggct
      301 cgccatggac ctaggagaac acgcagctat ttgcattggc aagatcgatc catatcggta
      361 caatgtaggc tccgatgaaa cgctcgcgat cccgcccgga tgacgtcctc ttcattgccg
      421 ccctggctgc cgtccttctg ggcatagagc tcctcctcta cacgacaggg gcctacaccg
      481 gcgccctcca ggtctggcca ttcagcgccc tcgtggccgg gggcctgctc ctctacctcg
      541 tcttcgtccg cgggttttcc cattacttct ttttcggcgg aattctcttc gaactcgtgg
      601 gcctcatatt catagccggc ggcctctccg gctggaccct gcgcgaggcc tggccccttg
      661 gcatggtggc ggccggcctc gcagggatcg cgggggggat cttccgctgg aggcgcttcc
      721 gcgcccgtta cgcggccccg tcggccggtt tcgtcgtcct cggctcggtc ttcgcccttt
      781 tctccttccg ggccatcacc atcgggtttg gccgcttcgt cgccgaatgg tggcccagcc
      841 tcctcattgc gggcggggtc ttcctctttg gagcctacgg cttcagcgcg cgaggggatt
      901 catcagtcag gggccagagg ccggagcgag gctcgggcaa gcaagccggc ggcgagcggc
      961 gttcggggag gacccgaagg ccctcaggcc gggaaggccc acagggctga ccatgttccg
     1021 gggccctgcg gtcgtgctgg cctcggttct cgtcgcggcc cttcttgcgg actcctgcgc
     1081 ctcccatcag gccggtcccc ccgatcccgc cggcagcccc gtcccaaccc caggccccca
     1141 ggccgatgcc gagccgcctc aggcaggtcc aggcaccgcg atccagataa gcgccgcgcc
     1201 gaaggccagc cgcgcccagg gcgatcgcgc ggcgatcgag gaagccatag tcctcgggtc
     1261 ccccgcctcg ctccagcggg ccaggtccct gctctcgtcc tcctccaacc tcaagcccga
     1321 ggagtcctcg gccctcggcc tggcgatcaa cgccttcgac gccctcatct atggctcggc
     1381 cgatgcggcc accctgctcg ccgtgggggt cccccccggt atgggcggcg tctccgccct
     1441 catcgccaag acggccgtcg acgcctttgc tgggcgggtt gacgatgccc cgcccgacta
     1501 cggggcatcc gggctggctg agctccttcc cgccctggcc atgttcgcct caccctcccg
     1561 cgacaccgcg agacaggccc aggatgccct cgagcgcttc tccggcctcg gctatgcctc
     1621 ggtgatcccc gacctggccc ttggcctcga cgccgagcgg aggcttgact ggaaggctgc
     1681 cttggcccgc tacaccgagg ccctcgacct ggccgaggat gcctggccgg cgaggcttgg
     1741 ggtggcgcgt tccgaactcg ccctcggcca ggccgttgaa gccctggcga tcctcgggcc
     1801 cctcgccgac aggctctcgg gctcgagcgc cttcatcagg acctacgccg aggcccttta
     1861 cgagaacgcg cgcttcgccc aggccgacca gtacgtggcc agggtcctcg tcgacgatcc
     1921 ccagaactcg cgcttcatcc tcattcgcgc ccacatcctc gtgcggagcg gctcctacca
     1981 gcaggccgtg cccctccttg acgcctacgc caccgtggat tcctccaacc gcctcttcct
     2041 cctcctgcgc agccaggtcg ccgagggcat gcgggacagg gacgacgccc tgcgctgggc
     2101 ccgccggggc ctggctgcct atgcggacga cccggagctc ctggtgacgg ccgcccggct
     2161 gcgtttctcg ggcccatccg caggacggca ggaggcccgg agcctggcgg cgcgggccta
     2221 cgagctcacc cccccggccc agggcagcga gggttttgcc ccggtccagc tcgaggcccg
     2281 gaacgccgcg gccttcgacg cggccaggct cctcgccgcc gacgccgcgg cgaggttcga
     2341 atggctgcct gccgcgcagt accttgagcg ggccacatcc tacgggagct tcgaggaccg
     2401 cgccctcgcc tccaccatcc tcaggcgttc gggcagggcc gcggccgccc tcgagtacgc
     2461 ggactcatgg taccgcgaca ggcccgagtc cgagg
//
LOCUS       DASSQV010000088         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1390666, whole genome shotgun sequence.
ACCESSION   DASSQV010000088 DASSQV010000000
VERSION     DASSQV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390666"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VFL04_01615"
     CDS             <1..981
                     /locus_tag="VFL04_01615"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7838426.1"
                     /translation="VLEPSEHRLEAPCPIYGECGGCNLMHLSYQRQVEAKSAIVSEAL
                     RRNARLEYGVVPTVASIPFAYRNRVQLHFSPEGRVGYMRRGSAEVIEASTCPIALKPI
                     QTWIEERAGSDRGWSELREWVMGKERFLAFGYGNEVWIEGRQGEVEVRVAGEPIHFHL
                     RGFFQSNLYMLDHFVADLVEGLSGDRAADLYSGVGLFGRFLAKAFSRVVCVEENPFAL
                     GLARKNVPGEGHEFSSMSIEDWVASPAAAQRFDCVLVDPPRTGLSAPVRRWLIGKKVP
                     QLAYASCDPVTLARDIAELVGAGYRLEWVKGFDFYPQTSHVECNARLSWA"
     gene            1205..2884
                     /locus_tag="VFL04_01620"
     CDS             1205..2884
                     /locus_tag="VFL04_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7838427.1"
                     /translation="MAGPVMDGVLRPPAAWMLSEDGALYALTESGNLAARVALPDPAA
                     AFLETDAFGRALVLLEGGHLAAYTRIGSRAWDFGLGTGTLWGLASGSDGRLFVAAGDR
                     LSCLNPGGRPLWTAILPSQPSAPPAVDGRGGPCVGLSDGSLSFFDGYGNPLGRVALGS
                     PARCLAPIPFEPVGALSPAGPPAAGVGAGPGQAPTPSSRALSPIPSRALSPTRSPILA
                     AGCSDGRIRILGSGPTILAEAAVSGLPIAGLVSDGNLVYALDAGGAVFALDSAARLVW
                     KTETSCRDGALALFRERLLVSGTGRAVSLSRGGEVFREISIANAIGRALPSPSGLLFS
                     AGADWVLAAYRFERPLGPQLIAATAPYPMPDAIGTELLLYDPRVREGDRQLALLSDIE
                     KSLGSASIGPEEGRAAAFCSAVALGQLEEVYPDAERRFRGNPLARAMACAILGELGSP
                     AYRPALISVLRTDGDPAVRSVACDALGAIGVDPGGLTGQAFLDAASRPVDEGTAVSLI
                     AAVERMALRSGTPPTIEAIRAVLKLANLPYGAFVRDRALAALGRMAGAASP"
     gene            2900..>3492
                     /locus_tag="VFL04_01625"
     CDS             2900..>3492
                     /locus_tag="VFL04_01625"
                     /inference="COORDINATES: protein motif:HMM:NF017106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HET7838428.1"
                     /translation="MKRIALVLLLMASAACWALDVVTGELESAKNSKVVFVSYEGPPA
                     KVESLAAIKGIGSALGAAARSGSLRAGAEARYAVIRVVDPTVKTGFDADIIVLGSDAQ
                     VDHIRNLRWIIAAYLSSAWGYSDADASLLATFVTVYNAVHRGDMGYIGSRYKPAVARE
                     LSPENAGLSTRYSDWPGKTRILIPLTEGATPGSLGAVS"
BASE COUNT          475 a         1262 c         1186 g          569 t
ORIGIN      
        1 gtcctcgagc cgagcgagca ccgcctcgag gcgccctgcc cgatctacgg cgagtgcggc
       61 ggctgcaacc tcatgcacct ctcctaccag cgccaggtcg aggcgaaatc ggccatcgtc
      121 tccgaggccc tgcggcggaa cgcccgcctc gagtacgggg tcgtgcccac ggtcgcctcg
      181 atccccttcg cctaccgcaa ccgcgtccag ctccacttca gccccgaggg cagggtcggc
      241 tacatgcgcc gcggctcggc cgaggtgatc gaggcctcga cctgccccat cgccctcaag
      301 cccatccaga cctggatcga ggagagggcg ggcagcgacc gcggctggag cgagctccgc
      361 gaatgggtca tgggcaagga gcgcttcctc gccttcggct acggcaacga ggtctggatc
      421 gaggggaggc agggcgaggt ggaggtcagg gtggctggcg agccgatcca tttccacctg
      481 cgcggctttt tccagagcaa cctgtacatg ctggaccact tcgtcgccga cctggtggag
      541 ggcctgtctg gggacagagc tgccgacctc tactcaggcg tcggcctttt tggccgtttc
      601 ctcgccaagg ccttctcgcg ggtcgtctgc gtcgaggaga atcccttcgc cctcggcttg
      661 gcccggaaga acgtgccggg ggagggccac gagttcagct ccatgtcgat cgaggactgg
      721 gtcgcctctc ccgccgccgc gcagcgcttt gactgcgtcc tggtcgaccc gccgcggacg
      781 gggctctcgg ccccggtgcg gaggtggctt atcgggaaga aggtccccca gctcgcctac
      841 gcctcctgcg acccggtgac cctcgcgcgc gacatcgccg agcttgtggg ggccggctac
      901 cgtctggaat gggtgaaggg cttcgacttc tatccccaga ccagccacgt ggagtgcaat
      961 gcgcgcctct cctgggccta agacccggag ctctgtcccc gccccagtcc caagcctcgc
     1021 tcctatggtc ttgcttgtcg ccgtcctgct gtggggacag agctcgaatc ccgctcgggg
     1081 agagggcccc atcggggggc cgggctcgat caggggaccg acggggggaa tcgggggaca
     1141 gagctcaacg tccgccgtcc aggctctcga gccggccttc cgcctgcctg caggcggaag
     1201 gccggtggcc ggaccggtca tggacggcgt cctgaggcct cccgccgcct ggatgctctc
     1261 agaggacgga gccctctacg ccctgaccga gtcgggcaac cttgcagccc gcgtggccct
     1321 gccggaccca gccgccgctt tccttgagac cgatgccttc ggaagggccc tcgtcctgct
     1381 cgaggggggc catctcgccg cctacacgag gatcggcagt cgggcctggg atttcgggct
     1441 tgggaccggg acgctgtggg ggctggcctc aggatcggat ggccggctgt tcgtcgccgc
     1501 cggagaccgg ctgagctgcc tcaaccccgg gggcaggccc ctctggaccg caatcctgcc
     1561 gagccagccc tccgcccccc ccgccgtcga cggccgtggc gggccttgcg tgggcctctc
     1621 cgacgggagt ctgtcctttt tcgacggcta tgggaatcct ctgggccggg tggcgctcgg
     1681 ctcgccggcg cgctgtctcg cgccgatccc ctttgagccg gtcggagctc tgtcccccgc
     1741 ggggccccct gcggctgggg tgggggccgg gccaggacag gctccaaccc cttctagccg
     1801 agctctgtcc ccaatcccga gtcgagctct gtccccaacc cggtccccaa tcctcgccgc
     1861 aggctgctcg gatggccgga tccgcattct cggctcaggc ccaaccatcc tcgccgaggc
     1921 ggccgtctcg gggctgccga tcgccggcct cgtcagcgac gggaacctgg tgtacgcgct
     1981 ggacgctggg ggcgcggttt tcgccctgga ctccgcggcg aggctggtct ggaagacgga
     2041 gacatcctgc agggacggag ctctcgctct tttcagggag cgtctgctgg tctcgggcac
     2101 gggcagggcc gtctccctgt cccgaggcgg cgaggttttc cgcgagatct cgatcgccaa
     2161 cgccatcgga cgcgccctcc catcgccctc gggcctcctc ttttctgccg gcgccgactg
     2221 ggtccttgcc gcctaccgct tcgagaggcc gctcgggccg cagctcatcg ccgccacggc
     2281 cccatatccg atgcccgacg ccatagggac agagctcctc ctctacgatc cgcgggtcag
     2341 ggagggggat cggcagctcg ccctcctttc cgatattgaa aaaagcctgg gatcggctag
     2401 tattggtccg gaagagggaa gggcggccgc gttctgctcg gcggtggccc tcggccagct
     2461 cgaggaggtc tatccggacg ccgagcgcag gttcaggggg aaccccctcg cccgcgccat
     2521 ggcctgcgcc atcctgggcg agctgggctc gccggcctac cgaccggccc tcatcagtgt
     2581 gctcaggacc gacggcgatc cggcggtccg ctccgtcgca tgcgacgcgc tcggcgccat
     2641 cggggtcgac ccgggcgggc tcacgggaca ggccttcctc gacgccgcgt cccggcctgt
     2701 cgacgagggc accgcggtgt cgctcatcgc ggcggtggag aggatggcgc tccgctccgg
     2761 gaccccgccc acgatcgagg cgatcagggc ggtgctcaag ctcgcgaacc tgccctacgg
     2821 ggccttcgtc cgggaccgag ccctcgccgc cctcgggcgg atggcgggcg cggccagccc
     2881 ctgaacttag gaggaacgaa tgaagcgcat cgccctggtc ctgctcctca tggcatcggc
     2941 ggcgtgctgg gcgctcgacg tcgtcaccgg cgagctcgag tcggccaaga acagcaaggt
     3001 cgtcttcgtc agctacgagg ggccgcctgc gaaggtggag tccctcgcgg cgatcaaggg
     3061 catcggctcg gcgctcgggg ccgccgcccg ctcggggagc ctcagggccg gggccgaagc
     3121 gcgttatgcg gtcatccgcg tcgtcgatcc gacggttaag acgggcttcg acgccgacat
     3181 catcgtcctc ggcagcgacg cccaggtcga ccacatccgc aacctccgct ggatcatcgc
     3241 ggcctacctc tcctcggcat ggggctattc ggacgccgac gcgagcctcc ttgccacctt
     3301 cgtcaccgtc tacaacgccg tccaccgcgg cgacatggga tacatcggct ccaggtacaa
     3361 gccggcggtc gccagggagc tcagccccga gaacgccggg ctctcgacgc gctactccga
     3421 ttggccgggc aagacgagga tactcatccc cctcaccgag ggcgccaccc cgggaagcct
     3481 cggcgcggtg tc
//
LOCUS       DASSQV010000089         3333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1018818, whole genome shotgun sequence.
ACCESSION   DASSQV010000089 DASSQV010000000
VERSION     DASSQV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018818"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /locus_tag="VFL04_01630"
     CDS             <1..1838
                     /locus_tag="VFL04_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838429.1"
                     /translation="TDFVVTSVVKDFLRYGDTFHLLSFGDTAQVEIAQRMTDERDVKS
                     VLGRLYLLYPLARYTDFIGALGYLYQYLADLPESRQKVAVVITDGVHNPPPGSPTYGY
                     PPEKVAAEVESVAAKIRAQGWPVHFIKLPFPAKAAAGGAKAGGSSGGATGAGSGAATA
                     GPQPTGSEAKAGQSYVDAAAKALGADIAQFSTETKTEVARQSLSLPSVEFPGPLGKRE
                     YSFSFPIKVTNSSPSQLGLELDRVRFGELDILSKKAFLSLAPGRSGTMSVPVRLPDSL
                     SAGEQRIEVELYFANGIRTSPQSGELALDLERSPVADFFRSGARLALFALLLVIGLGL
                     ALAAIIFLRRLPRRAAEPVVAAVRENSGIPAATGRSGARKTAAAAPAAPAQTASAPRP
                     AAAAAEAAGTELSAASSRARSDAKDRDAAVADAAEAIAAAKRAEAERSAALLAEAAGR
                     KAPVAAQRQAKVAAAKEKPRPAGSPSYTPRVRRPGSVQIELRVEEQNPHIGTRNVQSL
                     QAGSSRSVGGGRSDFLVFLVPIPARVAELHYDGEDLTLVPLKAELFPGGSAPIEGCLG
                     KDIRMLSPGGYPLTLRFLHYERPEARINRLLHCIEAPGLFKDPEA"
     gene            complement(1835..3199)
                     /locus_tag="VFL04_01635"
     CDS             complement(1835..3199)
                     /locus_tag="VFL04_01635"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET7838430.1"
                     /translation="MRAQSPQKSAAVLLSVIMCALCATGIGGQTPGPAPSPDKVEADR
                     RDTIRYGIDTEVIGLLSDLASEKEARYNADLLELMDRTRNAKLRQSILDFFAGSEWKG
                     AEASALKLVDQRDQEDAGLVASALSYLAAVRSHDALRYAKDIIKEDDKKLLGPLMKLL
                     GRAGGPDEEGILLSWFEGDAVADNLREDAVRALGDIGSGKAAKRLGEIAADSTRSKGL
                     RMLACEALGKIKDPASLGDLTKAANGDDPNVRTSAIEAMPAFASAEADAVIVEALRDS
                     YAKVRIAACKGVGLRVITAALPILRYKAASDPEKAVRGEALRGMAVLGGEAFAFLRER
                     MDDPKESPVFRVQCFGLLARKDAQGSMKALEARLSAEAASKDRSLYTGFAKEIANADD
                     APGAAGLARILLSDKDYLIRVAGVEWIRKNKDSSFKAELERLSKEDPAEMIRKRAEAA
                     LAAL"
BASE COUNT          480 a         1228 c         1108 g          517 t
ORIGIN      
        1 tcaccgactt tgtcgtcacc tcggtggtca aggacttcct ccgctacggc gacaccttcc
       61 acctcctgag cttcggcgac accgcccagg tcgagatcgc ccaacgcatg accgacgagc
      121 gcgacgtcaa gtccgtgctc ggcaggcttt acctccttta ccccctcgcc cggtacacgg
      181 acttcatcgg ggccctgggc tacctctacc agtatctcgc cgacctcccc gagagccgcc
      241 agaaggtcgc cgtcgtcatc accgacggcg tgcacaaccc gcctcccggc agccccacct
      301 acggctatcc tcccgagaag gtcgcggccg aggtcgagtc ggtggcggcc aagatcaggg
      361 cccagggctg gcccgtccac ttcatcaagc tccccttccc cgctaaggcg gcggcgggag
      421 gcgcgaaggc gggcggcagc tccggcggcg ccacgggcgc cggctcggga gccgccacgg
      481 ccggccccca gccgacgggc tctgaggcca aggcagggca gtcctatgtc gacgccgcgg
      541 cgaaggccct cggagccgac atagcccagt tctcgaccga gaccaagacc gaggtcgcgc
      601 gccagagcct gtcccttccc agcgtcgagt tccccggccc cctgggcaag cgcgagtact
      661 ccttctcctt ccccatcaag gtgaccaact cctcgccctc ccagctcggg ctcgagctcg
      721 acagggtgcg cttcggcgag ctcgacatcc tgtccaagaa ggccttcctc agcctggccc
      781 cggggaggag cgggaccatg tcggtccccg tgcgcctgcc cgattcgctc tccgcgggcg
      841 agcagaggat cgaggtggag ctctatttcg ccaacgggat caggacgagc cctcagagcg
      901 gcgagctcgc cctcgacctc gagcgctccc cggtcgccga cttcttccgc tcgggggcca
      961 ggctcgcgct cttcgcgctg ctcctcgtca taggcttggg cctcgccctc gcggccatca
     1021 tcttcctccg aaggcttccg aggcgggcgg ccgagcccgt cgtcgccgcg gtgcgcgaga
     1081 actcgggcat cccggccgcc accggccgaa gcggggccag gaagaccgcg gccgcggccc
     1141 cggccgcccc ggcccagacg gcctccgcgc ccagaccggc cgcggcggcg gccgaggccg
     1201 ccgggacaga gctctccgcc gccagctcta gggcgaggtc ggatgccaag gaccgcgatg
     1261 ccgcggtcgc cgacgcggct gaggccatcg ccgctgcgaa gagggcggag gccgaacgct
     1321 cggccgccct cctcgccgag gccgccggga ggaaggcgcc cgtggcggct cagcggcaag
     1381 ccaaggtcgc agccgccaag gagaagccca ggcccgccgg ctctccctcc tacacgccca
     1441 gggtcagacg gcccggcagc gtccagatcg agctcagggt cgaggagcag aacccccaca
     1501 tcggcacgag gaacgtccag agcctccagg ccggctcgtc acggagcgtc ggcggcgggc
     1561 gcagcgactt cctggtcttc ctggtgccga tccccgcgcg ggtggccgag ctccactacg
     1621 atggggagga cctgaccctc gtgcccctga aggccgagct cttcccgggc ggcagcgccc
     1681 ccatcgaggg ctgcctcggc aaggacatca ggatgctgag cccaggcggc tatcccctca
     1741 ccctccgctt cctccactac gagaggcccg aggccaggat caaccgcctc ctccactgca
     1801 tcgaggcgcc ggggctcttc aaggaccccg aggcctagag ggcggcgagg gcagcctcgg
     1861 cgcgcttcct gatcatctcc gcgggatcct ccttcgagag ccgttcgagc tcggccttga
     1921 aggaggagtc cttgttcttc cggatccact ccacgccggc gaccctgatc aggtagtcct
     1981 tgtccgagag caggatgcgg gcgaggccgg ccgccccggg tgcgtcgtcg gcgttcgcga
     2041 tctccttggc gaagcccgtg tagaggctgc ggtccttcga ggccgcctcg gccgacagcc
     2101 tcgcctccag ggccttcatg gagccctgcg cgtccttgcg ggccaggagg ccgaagcact
     2161 ggacgcggaa gacggggctc tccttggggt cgtccatccg ctcgcgcagg aaggcgaagg
     2221 cctccccgcc gaggactgcc atgccccgca gggcctcgcc gcggaccgcc ttctcgggat
     2281 cgctcgcggc cttatagcgc aggatgggca gggccgcggt gatcacgcgc agccccacgc
     2341 ccttgcatgc cgcgatcctg accttggcgt atgagtcgcg gagggcctcg acgatgacgg
     2401 cgtcggcctc ggcgctcgcg aaggccggca tggcctcgat ggccgaggtc ctcacgttcg
     2461 ggtcatcgcc gttggccgcc ttcgtgaggt cgccgaggct cgctgggtcc ttgatcttgc
     2521 cgagggcctc gcaggcgagc atcctgaggc ccttgctccg cgtggagtcg gccgcgatct
     2581 cgccgaggcg tttggccgcc ttgcccgaac ctatgtcgcc cagggccctc accgcatcct
     2641 ccctgaggtt gtcggccacg gcgtcgccct cgaaccagga gaggaggatc ccctcctcgt
     2701 cgggtcctcc cgcgcggccc aggagcttca tgagggggcc gaggagcttc ttgtcgtcct
     2761 cctttatgat gtccttcgcg tagcggaggg cgtcgtggga gcggacggcc gcgaggtagg
     2821 acagggccga ggccacgagg ccggcatcct cctggtcccg ctggtccacg agcttcagcg
     2881 ccgaggcctc ggcgcccttc cactcgcttc cggcaaagaa atcgagtatg gactgtctga
     2941 gcttcgcgtt gcgcgtcctg tccatgagct cgaggaggtc ggcgttgtag cgggcctcct
     3001 tctcggaggc caggtcggac agcaggccga tgacctccgt gtcgatgccg tagcggatcg
     3061 tgtcccgcct gtcggcctcg accttgtcgg ggctcggcgc cggccctggc gtctggccgc
     3121 caatcccggt tgcgcacaac gcgcacatga tcacggaaag gaggacggcc gcggacttct
     3181 gcgggctttg ggccctcatg catgaacctc ccatccaggg ccgccgcttt agtcctccag
     3241 ggccgaacca cggtaggcgg cgaggaagct gcgcttgaag agctcgaact ggccctgctc
     3301 gatactatag cgtatgtcgc ggacgaggtc att
//
LOCUS       DASSQV010000090         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2014301, whole genome shotgun sequence.
ACCESSION   DASSQV010000090 DASSQV010000000
VERSION     DASSQV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014301"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..523
                     /locus_tag="VFL04_01640"
     CDS             77..523
                     /locus_tag="VFL04_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HET7838431.1"
                     /translation="MRLWTLHPRYLDAKGLVAAWREGLLAQRVLAGGTRGYRSHPQLE
                     RFRASAEPLAAVATWLAQVLAEAERRSYSFDASRIAEPGRAALGSIPAKAGQLEYEAR
                     LLGRKLAARDPLRQRELAAQVRLEPNPLFRIVPGGVEGWERAIPGL"
     gene            complement(530..1909)
                     /locus_tag="VFL04_01645"
     CDS             complement(530..1909)
                     /locus_tag="VFL04_01645"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HET7838432.1"
                     /translation="MRKVSIAWMAAAGAILLSLALGCAPAAQGPQESEGEAVAFDQLL
                     ANLDALGASPARAALAASTIPKPNGSPFTAPELQSYLSGLKVDLAGLVAAKTGGRPYL
                     VLPVRLQAGGQSGLMWVPVSLGRRLSCPVIAFQHGTQTYWASAPSRFDLNPLSILSSF
                     DVSGAFQNYVECTTAALMASAGYIVVMADYPGFGSSRAPHPYVNLVLGSSVQTMLLRA
                     QAILAGPLASARPNGRLYLIGYSEGGFATLAAAKALQESGVAVTAAVPCAGSYDMSGA
                     EIADILSGRPALEPFYAPYTLYGYASVYASLDPGVWDFAAVLQPPLAGLMPALFSGNE
                     TVAMINAQLPGDIPRSFLSPGLLAELSVPTGRVYERLRETTVFLGWAPRMLVQLIQCP
                     EDDLVPPANTLAAKAAWAALPNVLEPIWVPPVPLPAALSGALDIHTRAFPTAMLAGFG
                     FIAGVEARF"
BASE COUNT          300 a          669 c          767 g          273 t
ORIGIN      
        1 ccaggaccgc tacctcaagc acgaggcccg gctcgccgcc caggccggca tggtcctcga
       61 gctctaggag ggggccttga ggctctggac cctccatccc cgctacctcg acgcgaaggg
      121 cctcgtcgcc gcctggcgcg agggcctcct cgcccagagg gtcctcgccg gcgggacccg
      181 gggctaccgc agccaccccc agctcgagcg cttcagggcc agcgccgagc ccctggcggc
      241 ggtcgccaca tggctcgccc aggtcctcgc cgaggccgag aggcggtcct acagtttcga
      301 tgcctcgcgc atagccgagc cgggccgggc agccctcggc tcgatccccg cgaaggcggg
      361 ccagctcgag tacgaggcca ggctcctggg ccggaagctc gctgcccgag accccctcag
      421 acagcgggag ctagcggccc aggtccggct cgagccgaac cccctcttcc gcattgtccc
      481 cggcggggtc gagggctggg agagggcgat ccccggtctc tgattccgcc tagaacctcg
      541 cctcaacacc ggcgatgaag ccgaagcccg cgagcatggc cgtggggaag gcgcgcgtgt
      601 ggatgtcgag cgcccccgag agcgcggccg gcagggggac gggcggcacc cagatcggct
      661 cgaggacgtt gggcagggcg gcccaggcag ctttcgctgc gagggtgttc gcgggcggga
      721 cgaggtcgtc ctcggggcac tggatcagct ggacgagcat ccgcggcgcc cagccgagga
      781 agacggtggt ctcgcggagc ctctcgtaga ccctgcccgt gggcaccgag agctcggcga
      841 ggaggccggg gctcaggaag ctcctcggga tgtcgccggg cagctgggcg ttgatcatcg
      901 cgacggtctc gttgccgctg aagagggctg gcatgaggcc ggccaggggg ggctggagga
      961 cggcggcgaa gtcccagacg cccgggtcga ggctggcgta gaccgatgcg tagccgtaga
     1021 gcgtgtaggg ggcgtagaaa ggttcgaggg ccggtctgcc cgagaggatg tcggcgatct
     1081 cggctcctga catgtcatag gagcccgcgc agggcacggc cgcggtcacc gccacccccg
     1141 actcctggag ggccttcgcc gccgccaggg tggcgaagcc cccctcggag tagccgatga
     1201 gatagagcct gccgttgggc ctcgcgctcg cgagggggcc ggcgaggatg gcttgggccc
     1261 tgaggagcat cgtctggacc gatgagccca ggaccaggtt cacgtagggg tggggagccc
     1321 tgctcgagcc gaagccggga tagtcggcca tcaccacgat gtagccggcc gaggccatga
     1381 gggccgccgt ggtgcactcg acatagttct ggaaggcgcc cgagacgtcg aagctcgaga
     1441 ggatcgagag gggattgagg tcgaagcgcg agggcgcgct cgcccagtag gtctgggtcc
     1501 cgtgctggaa ggcgatcacc gggcaggaga gcctccggcc caggctgacc ggaacccaca
     1561 tgaggccgct ctggcccccg gcctggagcc tcacggggag gacgaggtat ggcctgcccc
     1621 ccgtcttcgc cgcgacgagg cccgccaggt cgaccttgag gcccgacagg tagctttgga
     1681 gctcaggggc ggtgaagggg gagccgttcg gtttggggat cgtggaagcg gccagggccg
     1741 ccctggcggg gctggcgccg agggcgtcca ggttggccag gagctggtcg aaggcgacgg
     1801 cctcgccctc gctttcctgc ggcccctggg ccgccggcgc gcagccgagg gcgagggaca
     1861 ggaggatggc gcctgccgcg gccatccagg ctatcgacac ctttctcatg ggaacctcct
     1921 tcgcgctggc catcgggggc cgcgcgcgac tgagtactcc gcgtcccgcc ccttcggggc
     1981 ggggtaagtt gttcgatagc cgaacccta
//
LOCUS       DASSQV010000091         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1640796, whole genome shotgun sequence.
ACCESSION   DASSQV010000091 DASSQV010000000
VERSION     DASSQV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640796"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..2129
                     /locus_tag="VFL04_01650"
     CDS             147..2129
                     /locus_tag="VFL04_01650"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HET7838433.1"
                     /translation="MGALLAAGHAAAPLAIDLLMKLSLASAGALAAIGLVGRRRPGLG
                     FSIAAAALLLGLASSFVNAPLMAGLRLPGAPAWSARALAGRSDPGPRPAGPDGALGET
                     EPGGGPAASAREPEPGSPGEAGAWLGAFGLVWLLGVAANLVSRLAGLAAARRLLRGAR
                     PCTDPRLIAGAAAAAAAVHAREPGLLISGGSPIPYSAGLLRPSIVLPPDAAGYAEGRL
                     SAVLLHETMHAKRRDNLVAELLHLAASLAWMSPLPWRCLAEAMALREEVCDEAALRAG
                     MAPSTYAASLLEAARSLSARPGPEPEASAAGGKLERRILAVLSWGRGGTGLRGLRGAT
                     ALLVLVVAFGACLLVGGAAASPVPSPSSIVWQAASDPLTSSHSFAPTNGSFRLELGGG
                     LARIRLSYKGSSLELSLPEGALPARLPLGGRTRLGVPNGRFAEPEVRVPFILSGWNIW
                     NGRPSSVLAAAPGTVISSRDDRVLGPVIELDHGSGLRTRYALRRYGESSVRIGQRVAA
                     GSPIGSLGEGYPDDLPYLQFGILLDVGGGELLSLDPAPFMFRDAGNRALPLGSSALNA
                     AVWRADRAELGRLLALGLDPNRPAVDGSLPLEWTVMTQNVELARDLVAAGADPRGRTA
                     LGMVPGNANSGPTIARYAIETGNAALISVLAPGVEK"
     gene            2155..>2535
                     /locus_tag="VFL04_01655"
     CDS             2155..>2535
                     /locus_tag="VFL04_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838434.1"
                     /translation="MEAPRLGLEEGRELLADAIASVPPGREGLRDFYAEDLVLPTGLP
                     LHLISALLPGLVVEAGARVAATAAGRVLWRFPAGPVAQRRPGRKGELRSVLMRALRRV
                     AEGLIQLGWSSWAYLAFLAFGSRRA"
BASE COUNT          281 a          999 c          889 g          366 t
ORIGIN      
        1 ccgcgccgtg ctcgggacct tcttcgccaa ctcggcctcc agggccgtcg ccgccctcct
       61 cgaggcctcc gacctcaggc tgccggagga ggagttcgcg cgcctcgccg ccctgatcga
      121 gggggccgag agggaagggg actgagatgg gcgccctgct cgccgcgggg catgcggccg
      181 cccccctggc catcgacctc ctcatgaagc tgagcctcgc ctccgccggg gccctcgccg
      241 cgatcggcct tgtcgggcgg cgccgccccg gcctcggctt ctcgatcgcc gcggccgccc
      301 tcctcctggg cctggcctcg agcttcgtca acgctcccct gatggccggc ctccgtctcc
      361 ccggcgcccc ggcctggtcg gccagggccc tggccggcag atcggatccg gggcctcgtc
      421 cggccgggcc cgatggcgcg ttgggcgaaa ccgaaccggg aggaggcccg gccgcttccg
      481 cccgggaacc agagcccggc tccccagggg aggccggagc ctggctcggg gccttcggcc
      541 tcgtctggct cctcggcgtc gccgccaacc tcgtctcgag gctcgctggc ctcgctgccg
      601 ccaggcgtct gcttaggggc gcgaggccct gcaccgaccc gaggctcatc gctggcgccg
      661 cggcggccgc ggcggccgtc cacgcgcgag agccagggct cctcatctcg ggcggctcgc
      721 ccatccccta cagcgccggc ctcctcaggc cgagcatcgt gctgcccccg gatgccgccg
      781 gctatgccga ggggaggctc tctgccgtcc tcctccacga gaccatgcac gcgaaaaggc
      841 gggacaacct cgtggccgag ctcctccatc tcgcggccag cctcgcctgg atgtcgcccc
      901 tgccctggcg ctgcctcgcc gaggcgatgg cgctccggga ggaggtctgc gacgaggccg
      961 cgctccgcgc ggggatggcg ccgtcgacct atgccgccag cctcctcgag gccgcccgct
     1021 ccctctccgc tcggccgggg cccgagcccg aggcgagcgc ggcggggggg aagctcgagc
     1081 gccggatcct cgccgtcctt tcatggggcc ggggagggac ggggctccgg ggcctgcggg
     1141 gggccaccgc ccttcttgtc ctcgtcgtgg cctttggagc ctgtctcctt gtcggcggag
     1201 ccgccgccag ccccgtgccc agcccctcct cgatagtctg gcaggccgcc tccgaccccc
     1261 tcacctcctc gcacagcttc gcccccacga acggaagctt caggctggag ctcggcggcg
     1321 gtctggcgag gatacggctc agctacaagg ggagctcgct cgagctgagc ctccctgagg
     1381 gggccctgcc ggcgaggctg cccctcggcg gccggacccg gctcggcgtc cccaacggcc
     1441 gcttcgccga gcccgaggtg agggtgccct tcatcctctc gggctggaac atctggaacg
     1501 ggcggccctc gagcgtgctc gcggccgcgc ccggcaccgt catctcgagc cgcgacgaca
     1561 gggtcctcgg gccggtgatc gagctcgacc acggctccgg cctcaggacc cgctacgccc
     1621 tcaggaggta cggggagagc tcggtcagga tcggccaacg cgtggccgcg gggagcccca
     1681 tcggctccct cggggagggt taccccgacg acctgcccta ccttcagttc gggatcctcc
     1741 tcgacgtggg cgggggcgag ctcctgtccc tcgaccccgc ccccttcatg ttccgcgatg
     1801 ccgggaaccg ggccctgccc ctgggctcga gcgccctcaa cgccgccgtg tggagggccg
     1861 accgcgccga gctgggccgc ctcctcgccc ttggcctcga ccccaacagg ccggcggtcg
     1921 acgggagcct gcccctggag tggacggtca tgacccagaa cgtcgagctc gcccgcgacc
     1981 tggtggcggc gggcgccgat ccccggggga ggacggccct cggcatggtg ccgggcaacg
     2041 cgaactccgg gcccacgata gcgcgttatg cgatcgagac tgggaacgcc gctctcatct
     2101 cggtcctcgc ccccggcgtt gaaaagtgag gcgggggggc ctagactcgg aggcatggaa
     2161 gctcctcgcc tcgggctcga ggagggccgg gaactcctcg ccgacgccat agcctcggtc
     2221 cctcccggca gggaagggct cagggacttc tacgccgagg acctcgtcct cccgaccggg
     2281 cttcccctcc acctgatctc ggccctcctt cccggcctcg tcgtcgaggc cggggcgagg
     2341 gtggcggcca cggccgccgg gagggtcctc tggcgctttc ccgccgggcc ggtcgcgcag
     2401 cggcgcccag gccggaaggg cgagctccgc tccgtcctca tgagggccct ccgccgggtc
     2461 gccgagggcc tcatccagct cggctggtca tcctgggcct atctcgcctt tctggccttc
     2521 gggagccgga gggcc
//
LOCUS       DASSQV010000092         7197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1889690, whole genome shotgun sequence.
ACCESSION   DASSQV010000092 DASSQV010000000
VERSION     DASSQV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889690"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..579)
                     /locus_tag="VFL04_01660"
     CDS             complement(307..579)
                     /locus_tag="VFL04_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838435.1"
                     /translation="MAYDTQTVDEMIRIGRDRGLADGLCEELVGDFLRVTAETCREYV
                     LRRHRELQAENLKNVDIFPIIQAEIAQRRFAAPPHSERQIRRLIYG"
     gene            complement(595..1047)
                     /locus_tag="VFL04_01665"
     CDS             complement(595..1047)
                     /locus_tag="VFL04_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7838436.1"
                     /translation="MTVDRDRQYIRFTLGEGGYALPASSVLAVQETPSITRLHRAEKY
                     VVGHMEHFGAAIPVIDLRVWLELPFCGAGSERCALILSRARGDEAVLIAALADSVQEL
                     VELCPSAIERGDTLTQYGELRALTGMARCGGEYVAVLDPSFIFSSIGA"
     gene            complement(1299..2729)
                     /locus_tag="VFL04_01670"
     CDS             complement(1299..2729)
                     /locus_tag="VFL04_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7838437.1"
                     /translation="MSQTPYPEHPILLLDDDEGLRTRLGLLLADAGINNIVSTEDPDL
                     AMRLVLGNEVTLVFLDLFLPKANGETLLEKFTAGSPQVPVIVVTGDAEPERIVRCMRA
                     GAIDYLVKPVEKGRLLVSVWNGLAAVELRRELNGFRERVLEGTLERPETFADIVTADP
                     RMLDVFRYIEAIASSRHSVLIQGETGAGKELFARAVHVASRRAGAFVAVNAAGLDDAL
                     FSDALFGHRKGAYTGADTARAGLIKEAAGGTLFLDEIGDLEPRSQVKLLRLLQDGEYY
                     PSGSDIPLRSEARVVAATSRDLREAVRTGAMRSDLYFRLQTHPIRIPPLRERRGDIPL
                     LVRHFVAKSAESLGMAAPRVPDALLATLAAYDFPGNVRELEALVHDEVARSRNGELDA
                     ATVRERLAKEEGASGLERVALPAEGQSELPIALLGETFPTMREAEKALVTLALRKASG
                     NMSVAAGMLGISRQTLYKKVAEIDEM"
     gene            2821..3969
                     /gene="nagA"
                     /locus_tag="VFL04_01675"
     CDS             2821..3969
                     /gene="nagA"
                     /locus_tag="VFL04_01675"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET7838438.1"
                     /translation="MKADEFLLTNGRIVLPRAVLDEASVLVRGGRIKAVLEKDERLPQ
                     GPRRIDLGGALVSPGLIELHILGCGGIGFDDLGPNLDSGIEALRAARAFLRERGVTSF
                     VPTLLCREAELSALAAAIEASGIAEDELPGIYVEGPFVNQARRGGIPADTIREPDSAY
                     LGRLINLTRGRLKLMTVAPELPGSREIIARLQAVGVLPCLGHSDCRLDRIRLPDGRFS
                     ITHLFNAMSPFSHKEPGLAMLPFLDRRPYVELNGDGVHVNEAALRVCAAAIDPERLIL
                     VSDAVIAAGMPHGEYSYYGTRILSGEDGVRYADSGTLMGSNFLAPDVLRNWLAVTGSS
                     AENAVRMLSLTPAQALGMEGRRGAIAAGLDATLVVWEGEFEAVRTIYG"
     gene            4398..5375
                     /locus_tag="VFL04_01680"
     CDS             4398..5375
                     /locus_tag="VFL04_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7838439.1"
                     /translation="MKKSIIAVLAILMIVLVAMPGFSQAKLKVLFMPGVADPFYFTME
                     KGIRAAADKAGVDLTVAEYPKAWGPENQIPILQATIASGKFDLLLIAPTSNEALKAPL
                     KAIYNSGTEIITVDTFLGDGDYSKPSDYNFPLSYIGSDNELGGKQMAEHLANLVGKKG
                     KVYVQATNPDASSVQGRVKGFQAGVAEFPNMKLVGVEWCLDVQQKAQEQTLAALQKDK
                     DIVGIFGLNVFSAQGATQADINAGLSGAVKMCLWDATLDNIANLKKGNADLVLAQKPG
                     EMGALGIEWGVKYLKDKKNVKVPKKVIPGFEFFTRENVNSPAMQPFIYQ"
     gene            5461..6570
                     /locus_tag="VFL04_01685"
     CDS             5461..6570
                     /locus_tag="VFL04_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7838440.1"
                     /translation="MSEESAAAKGQGGRLGAGGGFLATGLGRSLANNWAACFLLIMLA
                     VFSMTGRNFLSIVNFQNVIHLQTVPLLLAAAETFVIISGGIDLSIGFVMGLVSVSAAK
                     LMQILLAAGLPVGWVIALATAAVLVGSLIPGLVNGIIITRFKVPPFIATMGMWGIANG
                     LALKLCEGFPVAGLPDALPEIGNGYLLYALPGQFLSLFGKPASFPDARIRELWRLIPY
                     SLPFILATLAALAYALRRKRFGRHTYAIGGSMDAAIRSGIDVDRHIIKIYALSSFLAG
                     VAGVFNVFQTGIGNYTTFSAMYELFAVAAVIIGGASLSGGKGRIWSSGVGVLVLAFLE
                     NGLAISGVLPFYRYIAVGVLLVIAVVIDKLFPNLR"
     gene            6583..>7197
                     /locus_tag="VFL04_01690"
     CDS             6583..>7197
                     /locus_tag="VFL04_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838441.1"
                     /translation="MNTQGTETAPRRAISAGAVRAFIGGRWVIAFLILETLYFCLRTE
                     GFANLNSAQILLFYGVQVFLLAVAELFVIVTGGIDLSVGYVLGFASIVSIKLIVFLTG
                     TGMPAPMALLIGVLVTLLIGLVPGLINGWLVAYLGVPPFIATFSMLGVCHGVSELLIN
                     GIYAMGLPQLAADIGNGRFFYLAPGAPLSFFSRPEVERGVRVLEV"
BASE COUNT         1157 a         2527 c         2296 g         1217 t
ORIGIN      
        1 ccatctacct aaaagcacat tgcgtgccaa gtgccccggc cttacggcga ggcgggacat
       61 ccttcatgca atccctgctg aggccgggaa atggctgcca ccacggcccc gcgcagaggg
      121 ggcaagccgg aggcgggctt cggcgcgggc ctggcgcagc ggggtcatgt gtacggtttc
      181 tggacggcgg cccgccccct gtccagttcc ccgacatggg gctgagcctg gaccaacagg
      241 cttcaaggga tacaaaaaag cgagaggccg ccttgcggcg gcctctcgag cgggcgagtg
      301 ctggccctat ccgtagatca ggcgcctgat ctggcgctcc gaatggggag gggccgcgaa
      361 tctccgctgc gcgatctcag cctggatgat cgggaagatg tccacgttct tcaggttctc
      421 agcctgaagc tcgcggtggc gccggagcac gtattcgcgg caggtctccg ctgtgaccct
      481 gaggaagtcc cccaccagct cttcgcagag gccgtcggcc aagcccctgt ccctgcctat
      541 cctgatcatc tcgtccaccg tctgcgtatc gtaagccatg tattcccccc cgccttaggc
      601 gcctatgctc gagaaaatga agctgggatc gagcaccgcg acgtactccc ccccgcacct
      661 ggccatgccc gtgagggccc gaagctcccc gtactgggtg agcgtgtcgc ccctctcgat
      721 cgccgaggga cagagctcga cgagctcctg gaccgaatcg gcaagggccg cgatgaggac
      781 ggcctcgtcc cccctggccc tcgagaggat gagggcgcag cgttcgctgc cggccccgca
      841 gaagggcagc tcgagccaga ccctgaggtc gatcacgggt atggccgcgc caaaatgctc
      901 catgtggccc acgacgtact tctcagcccg atgcagcctc gttattgaag gcgtctcctg
      961 gacagcgagg accgaagagg cgggaagcgc atagccacct tcgccgagcg tgaacctgat
     1021 gtactgtctg tccctgtcta ctgtcatggc gtcctccgat gcggagcggg ccgctggcct
     1081 cacccgaaac cgggtccact cccagagaca cttaagcaag ccgcatgcca tggccgctga
     1141 gagaaatgag gagatattgg atattttaat tatttacaga gcatggagtt gcgagaccga
     1201 gttcgggcag gagggcggag tgggctaggg cttcgggttt tggggacaga gctcgcgtca
     1261 aatttctgga cagcccccgc ggggactgtc cagattcttt acatctcgtc gatctcggcg
     1321 accttcttgt agagggtctg gcggctgatc ccgagcatcc ctgccgccac gctcatgttg
     1381 cccgaggcct tcctgagggc gagggtcacg agggccttct ccgcctcgcg catggtcggg
     1441 aaggtctcgc caaggagggc gatgggcagc tcggattggc cctcggcggg cagcgccacc
     1501 cgctccaggc ccgaggcccc ctcctccttc gccagccgct cccggacggt ggccgcatcc
     1561 agctcgccgt tgcgcgaccg cgccacctcg tcgtggacga gggcctcgag ctcgcggacg
     1621 ttccccggga agtcataggc ggcgagggtg gctagaaggg cgtccggcac cctgggcgcg
     1681 gccatgccga ggctctcggc cgacttcgcg acgaagtgcc gcacgaggag gggaatgtcg
     1741 ccgcgcctct ccctgagggg agggatccgg atcggatggg tctggaggcg gaaatagagg
     1801 tcgctccgca tcgcgcccgt cctcacggcc tccctcaggt cccttgaggt ggcggccacg
     1861 accctcgcct cggagcgcag ggggatgtcc gagcccgagg ggtagtactc cccgtcctgg
     1921 aggagcctga ggagcttcac ctgcgagcgg ggctcgaggt cgccgatctc gtcgaggaag
     1981 agggtgcctc cggcggcctc cttgatgagg ccggcgcggg cggtgtcggc gccggtgtag
     2041 gcccccttgc ggtgcccgaa cagggcgtcg ctgaaaaggg cgtcgtcgag cccggccgcg
     2101 ttcacggcga cgaaggcccc ggccctgcgg cttgcgacgt ggacggcccg ggcgaagagc
     2161 tccttgcccg ccccggtctc gccctggatg aggaccgagt ggcgggagct ggcgatggcc
     2221 tcgatgtacc tgaagacgtc gagcatccgg ggatcggcgg tcacgatgtc ggcgaaggtc
     2281 tcgggccgct cgagggtacc ctcgaggacg cgctcgcgga agccgttgag ctcccgcctg
     2341 agctcgaccg ccgccagacc gttccacacc gagacgagga ggcggccctt ctccaccggt
     2401 ttcacgaggt agtcaatcgc ccccgcgcgc atgcacctga cgatccgctc gggctcggcg
     2461 tctccggtca cgacgatgac gggcacctgg ggggagcccg cggtgaactt ctcgaggagg
     2521 gtctcgccgt tggccttggg gaggaaaagg tcgaggaaga cgagggtgac ctcgttgccc
     2581 agcacgagcc tcatggcgag gtcggggtcc tcggtcgaca cgatgttgtt gatgccggcg
     2641 tcggcgagga ggaggcccag cctggtgcgc aggccctcgt cgtcgtccag gagaaggatc
     2701 gggtgctcgg ggtagggtgt ttggctcatg gtgcgcttcg cagatgagtg tagccgaagc
     2761 cgtgtccggc cgcaatcgtg caccctcgac gcggagcccc gatggtggca tgatgcggcg
     2821 gtgaaagccg acgaattcct ccttacgaac gggcgcatcg tgcttccccg cgccgtcctg
     2881 gacgaggcct cggtcctcgt gcgcggcggc aggatcaagg ccgtgcttga gaaggatgag
     2941 cgcctccccc agggcccgcg gcgcatcgac ctcggcgggg ccctggtcag ccccggcctc
     3001 atcgagctcc acatcctcgg ctgcggcggg ataggcttcg acgatctcgg gccgaacctg
     3061 gactcgggga tcgaggccct gcgcgccgcg agggccttcc tcagggagcg cggggtcacc
     3121 agcttcgtgc cgaccctcct gtgcagggag gccgagctgt cggccctcgc cgcggccatc
     3181 gaggcctcgg gcatagccga ggacgagctc cccggcatct acgtcgaggg gcccttcgtg
     3241 aaccaggcca ggaggggcgg catccccgcc gacaccatcc gcgagcccga ctcggcctat
     3301 ctgggcaggc tcatcaacct gacccgcggc cggctcaagc tcatgaccgt ggcccccgag
     3361 ctgcccggct cgagggagat catcgccagg ctccaggcag tgggcgtcct gccctgtctc
     3421 ggccactcgg actgcaggct cgacaggatc aggctccccg acggccgctt ctcgatcacc
     3481 cacctcttca acgccatgtc ccccttctcc cacaaggagc cgggcctcgc catgctcccc
     3541 ttcctcgacc ggaggcccta cgtcgagctg aacggcgacg gggtccacgt gaacgaggcg
     3601 gcgctcaggg tctgcgccgc ggccatcgac cccgagcgcc tcatcctcgt ctccgacgcc
     3661 gtgatcgccg ccggcatgcc ccacggggag tactcctact acggcaccag gatcctctcg
     3721 ggcgaggacg gggtgcgcta cgccgactcg ggcaccctca tgggctccaa cttcctcgcc
     3781 cccgacgtgc tccggaactg gctggcggtg accggctcat cggccgagaa cgcggtgcgg
     3841 atgctgagcc tcacccccgc ccaggccctg ggcatggagg ggaggcgtgg ggcgatcgcc
     3901 gccgggctcg atgcgaccct cgttgtctgg gagggcgagt tcgaggcggt caggaccatc
     3961 tacggctagc aggccccggc cgccgagctc tgtccccgga ggctttggca accgagctcc
     4021 cgtccccgag gtcccgtccc cgaggtccgt cgccggcccg aaatggatac ccggctagcc
     4081 agggcgtggt taccctggta tccactcggg ctccggcacg ccctggccga gatggatgga
     4141 tcggcgccgc gatttattgt aatcgattac agcacaaggt tttgtcgggc caccagtttg
     4201 gagtttttgt caaagaatgc gagctcggga acatcccgac agcaaccgcg tcgcgcaact
     4261 tcagggaaac tccgggtctt gagccttttt gcagaaaact ccgaaatccg ctcccgcgcc
     4321 tggccgctgg cacggttccg gcaatgtggt ctgggccggt cttggtgccg gacaattcct
     4381 cgatcaggga gcgtgctatg aaaaagtcga tcatcgcggt cctcgcgatc ctcatgatcg
     4441 tcctcgtcgc gatgcctggc ttctcccagg ccaagctcaa ggtcctcttc atgcccggcg
     4501 tcgccgaccc cttctacttc acgatggaga agggcatcag ggccgcggcc gacaaggccg
     4561 gcgtcgacct cacggtcgcc gagtatccca aggcctgggg tcccgagaac cagatcccca
     4621 tcctccaggc caccatcgcc tccggaaagt tcgacctcct cctcatcgcc ccgacctcga
     4681 acgaggccct caaggccccc ctcaaggcga tctacaacag cggcacggag atcatcacgg
     4741 tcgacacctt cctcggcgac ggcgactact cgaagccgag cgactacaac ttccccctct
     4801 cctacatcgg ctccgacaac gagctcggcg gcaagcagat ggccgaacac ctcgccaacc
     4861 tcgtcggcaa gaagggcaag gtctatgtcc aggccaccaa tcccgacgcc tcctcggtcc
     4921 agggccgcgt caagggcttc caggccggcg tcgccgagtt cccgaacatg aagctcgtcg
     4981 gcgttgagtg gtgcctcgac gtccagcaga aggcccagga gcagaccctg gccgccctcc
     5041 agaaggacaa ggacatcgtc ggcatcttcg gcctcaacgt cttctccgcc cagggcgcga
     5101 cccaggcgga catcaacgcc ggcctctccg gcgccgtgaa gatgtgcctc tgggacgcga
     5161 ccctcgacaa catcgccaac ctcaagaagg gcaacgccga cctcgtcctg gcccagaagc
     5221 ccggcgagat gggcgccctc ggcatcgagt ggggcgtcaa gtacctcaag gacaagaaga
     5281 acgtcaaggt ccccaagaag gtcatccccg gcttcgagtt cttcacccgc gagaacgtca
     5341 acagcccggc gatgcagccc ttcatctacc agtaaggccc gatccaaaga aggcggaccg
     5401 gggggccgaa acaggccccc cgggctcgcc gtagccaacg acccaagcgg aaggaacacc
     5461 atgagcgaag aaagcgccgc ggcgaaggga caggggggga ggctcggggc cggcggcggc
     5521 ttcctcgcca cgggtctcgg gcgcagcctg gcgaacaact gggcggcctg cttcctcctc
     5581 atcatgctgg cggtcttcag catgacgggg cggaacttcc tctccatcgt caacttccag
     5641 aacgtgatcc acctccagac cgtccccctc ctcctcgcgg cggccgagac cttcgtcatc
     5701 atctcgggag ggatcgacct gtccatcggc ttcgtcatgg gcctcgtgtc ggtttcggcg
     5761 gccaagctca tgcagatcct cctggcggcc ggcctccccg tgggctgggt catcgccctc
     5821 gcgacggcgg ccgtcctcgt cggctcgctc atccccggcc tcgtcaacgg gatcatcatc
     5881 acccgcttca aggtcccgcc cttcatcgcg accatgggca tgtggggcat cgccaacggg
     5941 ctcgcgctca agctctgcga gggcttcccc gtcgccggcc ttcccgacgc cctgccggag
     6001 atcggcaacg gctacctcct ctacgccctc cccggccagt tcctcagcct cttcggcaag
     6061 ccagcctcct ttcccgacgc ccgcatcagg gagctctggc gcctcatccc ctactccctg
     6121 cccttcatcc tcgcgaccct cgccgccctt gcctacgcgc tgaggcgcaa gcgcttcggc
     6181 cgccacacct acgcgatagg cggcagcatg gacgcggcga tacgctcggg catcgacgtc
     6241 gaccgccaca tcatcaagat ctacgccctg tcctccttcc tcgccggggt agcgggagta
     6301 ttcaacgtct tccagacggg catcggcaac tacaccacct tcagcgcgat gtacgagctc
     6361 ttcgccgtcg ccgccgtcat catcggcggg gcgagcctct cgggcgggaa ggggaggatc
     6421 tggagctcag gcgtaggagt cctggtcctc gccttcctcg agaacggcct cgccatctcg
     6481 ggggtcctgc ccttctaccg ctacatcgcg gtcggcgtcc tcctcgtcat cgcggtggtc
     6541 atcgacaagc tcttcccgaa cctgcgatag gaggatgcgc acatgaacac acagggaacc
     6601 gagaccgcgc cgcggagggc cataagcgcc ggcgccgtgc gcgccttcat cggggggcgg
     6661 tgggtcatcg ccttcctcat cctcgagacc ctctacttct gcctcaggac cgagggcttc
     6721 gccaacctga actcggccca gatcctcctc ttctacgggg tccaggtctt cctcctggcc
     6781 gtggccgagc tcttcgtcat cgtcacgggc gggatcgacc tctccgtcgg ctacgtcctg
     6841 ggcttcgcct cgatcgtctc catcaagctc atcgtcttcc tgacggggac ggggatgccg
     6901 gcgcccatgg ccctcctcat cggggtcctg gtgaccctcc tcatcggact cgtccccggc
     6961 ctcatcaacg gctggcttgt ggcctacctc ggcgtgcccc ccttcatcgc caccttctcg
     7021 atgctcgggg tctgccacgg ggtctcggag ctgctgatca acggcatcta cgcgatgggc
     7081 ctgccccagc tcgcggccga catcgggaac gggcgcttct tctatctggc gcccggggcc
     7141 cccctctcct tcttctcgag acccgaggtg gagcgcgggg tcagggtcct cgaggtc
//
LOCUS       DASSQV010000093         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2139028, whole genome shotgun sequence.
ACCESSION   DASSQV010000093 DASSQV010000000
VERSION     DASSQV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139028"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1876
                     /locus_tag="VFL04_01695"
     CDS             <1..1876
                     /locus_tag="VFL04_01695"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7838442.1"
                     /translation="IVESLGTRLAASSVLLAKARILGRIDQEVALARSLADSPVVRAW
                     VLDEASAPKEARARAELESYRRHFADGSYFVAIASSGTYYNQGREGARSVVGLSEQRP
                     SDAWFWTTLKIVREVKLNLDFNPYVRQTKVWINCVMREGERPIAVAGSGIDISDLVQS
                     LTAEASNGFSLMLLDAAGNITAHRETGVLERNARAWSGSKTKAIDLAGSARDRLALSG
                     LLEGLASRPEDIHSARLGFEGSTRLVTASYLPGIDWIILAGVDLDRVLSLRNFAAIPA
                     AFIVALVLSLIAVAGLVDRLVLRPLAALDAAAARIAAGHYELALPAKAPGELGRLSFS
                     FARMAHAVRESTEDLELQVAHRTEALAEANARMLEDLRYADLVQRSILPEPEALRRGL
                     PRHFVIFRPRDRVGGDFYFFAESRGGFFVGVIDCTGHGVSGALMTMFSRSALELIAAR
                     TEASDPAAMVRALDEHLRAGLAEGAEGLGAERGLDIGLCRLEPRSNRLLFAGAGIDLF
                     VVEKGESPRLERVRTGHGSIGSAGRLRAAAPRGVEYPLAGRTFILSSDGFFDQAGGPK
                     GFGFGSTRFAELALSCSNCDMAERRSRFEAALAEWQGELAQRDDITVLAFEAGPSEGA
                     "
     gene            1879..>2193
                     /locus_tag="VFL04_01700"
     CDS             1879..>2193
                     /locus_tag="VFL04_01700"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HET7838443.1"
                     /translation="MAAPALDIYGLRKSLIESDILICFNGPFSHSIIEEIGTAVRRYL
                     ESDAAPSSAMTDVFAVFVELAQNVQAYTSSLRGADGGAGLDAGTLVIGRAGDRYALSS
                     GNA"
BASE COUNT          327 a          783 c          742 g          341 t
ORIGIN      
        1 catcgtcgag tccctcggca ccaggcttgc ggccagcagc gtcctcctgg cgaaggcccg
       61 catcctgggt cggatcgacc aagaggtggc cctcgcccgg agcctcgccg actcgcccgt
      121 ggtgcgggcc tgggtgctcg acgaggccag cgctcccaag gaggcccgtg ccagggccga
      181 gctggagagc taccgccggc atttcgccga cggcagctat ttcgtcgcca tagcctcgag
      241 cgggacctac tacaaccagg gccgtgaagg ggcacgctcg gtggtcggcc tgagcgagca
      301 gcggccctcc gacgcctggt tctggacgac cctgaagatc gtcagggagg tcaagctcaa
      361 cctcgacttc aacccctacg ttcgccagac gaaggtctgg ataaactgtg tcatgcgcga
      421 gggggagagg ccgatagcgg tcgcgggcag cggcatcgac atctccgacc ttgtccagag
      481 cctcaccgcc gaggcctcga acgggttctc gctcatgctc ctcgacgccg ctggcaacat
      541 caccgcccat cgcgagaccg gggtgcttga gcgcaacgcg agggcctgga gcggatccaa
      601 gacgaaggcc attgacctgg ccggttcggc cagggacagg ctcgccctgt cgggcctcct
      661 cgagggcctg gcctccaggc ccgaggacat ccacagcgcc aggctgggct tcgagggctc
      721 gacccggctc gtcacggcgt cctacctgcc cggcatcgac tggatcatcc tggcgggcgt
      781 cgacctcgac cgcgtcctgt cgctgaggaa tttcgccgcg atccccgcgg ccttcatcgt
      841 cgccctggtc ctttcgctca tcgcggtggc cggcctggtt gacaggctcg tcctgcggcc
      901 ccttgcagcc ctcgacgctg ctgcggccag gatagcggct ggccactatg agctggccct
      961 gccggccaag gcccccggcg agctgggcag gctgtccttt tccttcgccc gcatggccca
     1021 tgcggtgcga gagtccaccg aggacctcga gctccaggtc gcccaccgca ccgaagccct
     1081 cgccgaggcg aacgcgagga tgctcgagga cctccgctac gccgacctcg tccagcgatc
     1141 gatcctgccc gagcccgagg ccctccgcag gggactcccc cggcacttcg tcatcttcag
     1201 gccgagggac cgcgtcggcg gcgacttcta tttcttcgcc gagtccaggg gcggcttctt
     1261 cgtaggcgtc atcgactgca cggggcatgg tgtctcgggc gccctcatga cgatgttctc
     1321 gaggtcggcc ctcgagctga tcgccgcgag gacggaggcg tcggatccgg ccgcgatggt
     1381 ccgggccctg gacgagcacc tcagggccgg cctcgccgag ggcgccgagg gcctgggggc
     1441 cgagcgcggc ctcgacatcg ggctctgcag gctcgagccc cggtcgaacc gcctcctttt
     1501 cgccggagcc ggcatcgacc tcttcgtcgt ggagaagggg gagagcccca ggctggagcg
     1561 cgtgaggacg gggcacggct ccataggctc ggcggggagg ctcagggccg cggcgccccg
     1621 cggcgtcgag taccccctcg ccgggcggac cttcatactc agcagcgacg gcttcttcga
     1681 ccaggcgggc ggccccaagg gcttcggctt cggcagcacg cgcttcgccg agctcgccct
     1741 ctcctgctca aactgcgaca tggccgagcg tcggagccgc ttcgaggcgg ccctggcgga
     1801 atggcagggc gagctcgccc aacgcgacga catcacggtg ctcgccttcg aggcggggcc
     1861 gtcggaagga gcatgagcat ggctgcgcca gccctcgaca tctacgggct caggaagagc
     1921 ctcatcgaat cggacatcct gatctgcttc aacggaccct tctcgcacag catcatcgag
     1981 gagatcggca cggcggtgag gcgctacctc gagagcgacg ccgccccgag ctcggcgatg
     2041 accgacgtct tcgcggtctt cgtcgagctc gcgcagaacg tccaggccta cacctcgtcc
     2101 ctgcgcggcg ccgatggagg cgcaggcctt gatgcgggca ccctcgtgat cggcagggcg
     2161 ggcgaccgct atgccctgag ctcgggcaac gcc
//
LOCUS       DASSQV010000094         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1268771, whole genome shotgun sequence.
ACCESSION   DASSQV010000094 DASSQV010000000
VERSION     DASSQV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268771"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /gene="nuoC"
                     /locus_tag="VFL04_01705"
     CDS             <1..1484
                     /gene="nuoC"
                     /locus_tag="VFL04_01705"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF008728.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HET7838444.1"
                     /translation="RNEELRIKVALQGERPSVPTATTIWKNANFYEREVYDMFGLGFE
                     GHPNLSRILMPPWWEGYPLRKEHPARGTELGPFVMDEAEQERQLEALRFRPEDWGLKS
                     SDQGHEYFFVNVGPQHPSAHGVLRVVAQMDGEVIVDLVPEIGFHHRGAEKMGERQSWH
                     TFIPYTDRVDYLAGSMNELPYVLSVEKLAGIEVPERAQVIRVMMAELFRVISHLVYYG
                     TFSQDVGQMSPVFYTFNDRERGFDIVEAITGGRMHPGWFRIGGVAMDLPEGWDRMVGD
                     FIPYMRRRLKDYDAAIMRNRMFMARTQGVGALSLAEAIEWGATGHFLRACGLDWDLRK
                     KRPYSGYEHFDFDIPTAAHGDSYDRLQIHVEEMHQSLRIIEQCLANMPSGPYKSEHPL
                     AVPPRKDRTMHDIETLINHFLGVSWGPVIPPGEASVMVEATKGNNSYYLVSDGGTASY
                     RTRIRTPCFAHVQMTPVMSRGLTISDLVVILGSVDYVLADVDR"
     gene            1502..1996
                     /gene="nuoE"
                     /locus_tag="VFL04_01710"
     CDS             1502..1996
                     /gene="nuoE"
                     /locus_tag="VFL04_01710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HET7838445.1"
                     /translation="MLSLIERERIDKAATQAPDRRSVVCDALMVVQEARGWVSDQALA
                     EVAEALGMSRDEVEGVATFFELVFRRSVGKHVILICDSVSCWIKGYEALLSQLRARLG
                     IGPGETTSDGMFNLLPAGCLGACDKAPAMMVDEELFVGVDEAALDRILEGFRDGGHGN
                     AAHR"
     gene            1974..>2176
                     /locus_tag="VFL04_01715"
     CDS             1974..>2176
                     /locus_tag="VFL04_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HET7838446.1"
                     /translation="MATPLTARIRAEGTAVAEAVDLKAYEATGGYKGLRAALAMGSAR
                     VREAVSQAKLRGRGGAGFATGLK"
BASE COUNT          373 a          769 c          712 g          322 t
ORIGIN      
        1 agcgcaacga ggagctgcgg atcaaggtgg ccctccaggg cgagaggccc tcggtcccga
       61 cggccaccac catatggaag aacgccaact tctacgagcg cgaggtctac gacatgttcg
      121 gcctgggctt cgagggccac ccgaacctga gccgaatcct catgccgccg tggtgggagg
      181 gctaccccct gcgcaaggag caccccgccc gaggcaccga gctcggcccc tttgtgatgg
      241 acgaggccga gcaggagcgc cagctcgagg ccctccgctt caggcccgag gactggggcc
      301 tcaagagctc tgaccagggc cacgagtact tcttcgtcaa cgtggggccc cagcacccga
      361 gcgcccacgg cgtcctccgc gtcgtggccc agatggacgg cgaggtcatc gtcgacctcg
      421 tccccgagat cggcttccac caccgcgggg ccgagaagat gggcgagcgc cagtcctggc
      481 acaccttcat cccctacacc gaccgcgtcg actatctcgc cggctccatg aacgagctgc
      541 cctacgtcct ctcggtcgag aagctcgccg gcatcgaggt ccccgagcgg gcccaggtga
      601 tccgcgtcat gatggccgag ctcttccgcg tcatctccca cctcgtctac tacggcacct
      661 tcagccagga cgtcggccag atgtccccgg tcttctacac cttcaacgac cgcgaacgcg
      721 gcttcgacat cgtcgaggcg atcaccgggg gccgcatgca cccgggctgg ttccgcatcg
      781 gcggggtcgc gatggacctg cccgagggct gggacaggat ggtcggggac ttcatcccct
      841 acatgcggcg ccgcctcaag gactacgacg ccgccatcat gcgcaaccgc atgttcatgg
      901 cccgcaccca gggcgtcggc gccctctccc tggccgaggc catcgagtgg ggcgcgaccg
      961 gccacttcct ccgggcctgc ggcctcgact gggacctcag gaagaagcgc ccctacagcg
     1021 gctatgagca cttcgacttc gacatcccca cggccgccca cggcgactcc tacgacaggc
     1081 tccagatcca cgtcgaggag atgcaccaga gcctccgcat catcgagcag tgcctggcca
     1141 acatgccctc ggggccctac aagtccgaac atcccctcgc cgtgcccccc cgcaaggacc
     1201 gcacgatgca cgacatcgag accctcatca accacttcct cggggtgagc tgggggccgg
     1261 tgatcccgcc gggcgaggcc tcggtgatgg tggaggccac caagggcaac aactcctact
     1321 acctcgtgtc ggacgggggc acggcctcct accgcacgag gatcaggacg ccctgtttcg
     1381 cccacgtgca gatgaccccc gtcatgagcc gggggctcac gatatctgac ctcgtcgtga
     1441 ttctcgggag cgtggactac gtcctcgccg acgtcgaccg ctagaagatc ggagaaagcg
     1501 gatgctgagc ctgatagaac gcgaacggat agacaaggcg gccacgcagg ccccggaccg
     1561 gcggtccgtg gtctgcgacg ccctcatggt cgtgcaggag gcgaggggct gggtctccga
     1621 ccaggccctc gccgaggtgg ccgaggccct cggcatgagc cgggacgagg tcgagggggt
     1681 cgccaccttc ttcgagctcg tgttccgccg gagcgtgggc aaacacgtca tcctcatctg
     1741 cgactcggtc agctgctgga tcaagggcta cgaggccctc ctctcccagc tgcgcgcgag
     1801 gctcggcatc gggcccggcg agacgacgag cgacgggatg ttcaacctcc tgcccgccgg
     1861 ctgtctcggg gcctgcgaca aggcgccagc catgatggtc gacgaggagc tcttcgtcgg
     1921 cgtcgacgag gctgctctgg acaggatcct cgaggggttc cgggatggtg gacatggcaa
     1981 cgccgctcac cgctaggata agggccgagg ggacggccgt cgccgaggcc gtcgacctca
     2041 aggcctacga ggccacgggc ggctataagg gcctccgcgc cgccctcgcg atggggagcg
     2101 ccagggtccg cgaggccgtc tcccaggcca agctcagggg taggggaggg gcgggcttcg
     2161 ccaccgggct caagtg
//
LOCUS       DASSQV010000095         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1020444, whole genome shotgun sequence.
ACCESSION   DASSQV010000095 DASSQV010000000
VERSION     DASSQV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020444"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1248)
                     /gene="flhF"
                     /locus_tag="VFL04_01720"
     CDS             complement(16..1248)
                     /gene="flhF"
                     /locus_tag="VFL04_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HET7838447.1"
                     /translation="MQYFTEQARSHREALEVVRSKYGENAQILSHRTVRVGGFMGFFV
                     REAVEVTGYIKPEAKPASKPAAPLDLEEEKRRILAQARGDQALQQVLQEIRGIRERLD
                     EEQFSGVRAAAESEDHETVRRVDALLVMNDFSEGFRREAVARLRRDFSLERLEDAEAA
                     EAAILEWVGDSIEVWREQPARRPRILALVGPTGVGKTTTIAKLAAIYAVGIAGEKPRS
                     VRMITIDNYRIGARQQIETYGNIMGVPVSCVETADDLRKTIALHADADLILVDTVGKS
                     PRDAVKLAEMQRILAACGPSAEVHLALAATTKASDIAQILRQFEPFDYKAVVLTKLDE
                     TNRIGNVISALAERKKPLSYVTNGQRVPQDIERARVVRLLMNLEGFRVDRPHLEARYG
                     EEPAAAARDERSITSIRS"
     gene            complement(1252..>2574)
                     /locus_tag="VFL04_01725"
     CDS             complement(1252..>2574)
                     /locus_tag="VFL04_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HET7838448.1"
                     /translation="LLVSTATGIIVTRANSLGTFGADVTMQFAQNSKIYIVSAVVLGL
                     LGLLPGFPWYVLFPMAGLLALVGLRLARKQVADADRAALAAAGAKKTEAPADLSPVVP
                     LDPISLELGYGLIPLVDKDKGADLLERITRIRKESALDMGLVVPRIRIIDNMRLEPSE
                     YSLKIKGVQVGRGIIRLGYYLAINPGGITEEIPGEKTRDPTFDLPALWIAEDQRDRAE
                     RAGYTVVDAPSIIATHLTDVIKRHASEILGRQEVQAILDALKKDYPAVVEDAAKRLNL
                     GEMQKVFQGLLREQVSIRNMVAILETLADYAGVTKDASFLVEKARQSLGRQICLQYAD
                     HERSLRILTIEQTLEQKIIDSRVDTPGGVVSALEPSLHRQWIKALTRAVAAVQGKGWL
                     PIVLCSEGARALVKASTERELPDLVVLSVPEIVADIRVEAVGEIRLEG"
BASE COUNT          344 a          852 c          911 g          467 t
ORIGIN      
        1 cctggtcttc catgttcagc tccggatcga ggtgatgctg cgctcgtcgc gagcggcggc
       61 tgcgggttcc tccccgtagc gggcctcaag atggggcctg tccacgcgga aaccctcgag
      121 gttcatgagg aggcggacga cgcgcgccct ttcgatgtcc tgcggcaccc tctggccgtt
      181 cgtgacgtag gagagcggct tcttccgctc ggcgagggcc gagatcacgt tgccgatcct
      241 gttcgtctcg tccagcttgg tgaggacgac ggccttgtag tcgaagggct cgaactgccg
      301 caggatctgg gcgatgtcgc tcgccttggt cgtggccgcc agggccagat ggacctcggc
      361 gctcgggccg caggcggcga ggatgcgctg catctcggcg agcttgacgg cgtccctggg
      421 gctcttcccc accgtgtcca cgaggatgag gtcggcgtcg gcgtggaggg ctatggtctt
      481 cctgaggtcg tcggcggtct cgacgcagga gacgggcacg cccatgatgt tcccgtaggt
      541 ctcgatctgc tgtctcgccc cgatgcggta gttgtcgatg gtgatcatcc ggaccgagcg
      601 aggcttctcc ccggcgatgc ccacggcgta gatggcggcg agcttggcga tggtcgtcgt
      661 cttgcccacc cccgtcggcc cgacgagggc caggatccgc ggccgcctgg ccggctgctc
      721 cctccacacc tcgatcgagt cgccgaccca ctcgaggatc gcggcctcgg ccgcctcggc
      781 gtcctcgagc cgctcgaggc tgaagtcgcg cctgagcctg gccacggcct cgcggcggaa
      841 gccctcggag aagtcgttca tgacgaggag ggcgtccacc cgcctgacgg tctcgtggtc
      901 ctcgctctcg gcggcggccc tgacgcccga gaactgctcc tcgtcgagcc tctccctgat
      961 cccgcggatc tcctggagga cctgctggag tgcctggtcg cccctggcct gggcgaggat
     1021 gcgccgcttc tcctcctcga ggtccagcgg agccgccggc ttggaggctg gcttggcctc
     1081 gggcttgatg tagcccgtca cctcgacggc ctccctcacg aagaagccca tgaagccccc
     1141 gaccctgacc gtcctgtgcg agaggatctg ggcgttctcc ccgtatttcg agcgcacgac
     1201 ctcgagggct tcgcggtggg accgcgcctg ttcggtgaag tactgcatcg cctagccttc
     1261 caaacgtatc tcgcccacgg cctcgacgcg gatgtccgcg acgatctccg ggacggaaag
     1321 gaccacgagg tcgggcagct cccgctcggt gctcgccttc acgagggcgc gggcgccctc
     1381 cgagcagagc acgatcggca gccagccctt gccctggacc gccgcgacgg ccctcgtgag
     1441 ggccttgatc cactggcggt gaaggctcgg ctcgagggcc gagaccacgc cgccgggggt
     1501 gtcaacgcgg ctgtctatga tcttctgctc gagcgtctgc tcgatcgtga ggatcctgag
     1561 gctgcgctcg tggtcggcgt actgcaggca gatctgccgc ccgagggact gcctcgcctt
     1621 ctcgacgagg aagctcgcgt ccttcgtgac cccggcgtag tcggccaggg tctcgaggat
     1681 ggcgaccatg ttcctgatcg acacctgctc gcggaggagg ccctggaaga ctttctgcat
     1741 ctccccgagg ttgagccgct tggcggcgtc ctcgaccacg gcggggtagt ccttcttgag
     1801 cgcgtcgagg atcgcctgga cctcctggcg cccgaggatc tccgaggcgt ggcgcttgat
     1861 cacgtcggtc aggtgggttg cgatgatgct cggggcgtcg accacggtgt agccggcgcg
     1921 ctcggcccgg tccctctggt cctcggcgat ccacagggct ggcaggtcga aggtggggtc
     1981 cctcgtcttc tcgccgggga tctcctcggt gatgccgccg gggtttatgg cgaggtagta
     2041 gccgaggcgg atgatgcccc gcccgacctg gacccccttg atcttgagcg agtactccga
     2101 gggctcgagc ctcatgttgt cgattatgcg gatcctcggg acgacgaggc ccatgtcgag
     2161 ggcgctctcc ttgcggatcc gcgttatgcg ctcgaggagg tcggcgccct tgtccttgtc
     2221 caccagcggt atgaggccgt agcccagctc gaggctgatg gggtccaggg ggacgactgg
     2281 cgagaggtcg gccggggcct cggtcttctt ggccccggcg gccgcgaggg ccgccctgtc
     2341 ggcgtcggcc acctgcttcc gcgccagccg gaggcccacg agggcgagga ggccggccat
     2401 ggggaagagc acgtaccagg ggaagcccgg caggaggccg aggaggccga ggacgacggc
     2461 cgagacgatg tagatcttgg agttctgcgc gaactgcatg gtgacgtcgg ccccgaaggt
     2521 gccgagcgag ttcgcgcggg tcacgatgat gcccgtcgcc gtggacacga ggag
//
LOCUS       DASSQV010000096         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1518324, whole genome shotgun sequence.
ACCESSION   DASSQV010000096 DASSQV010000000
VERSION     DASSQV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518324"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2544
                     /locus_tag="VFL04_01730"
     CDS             <1..2544
                     /locus_tag="VFL04_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HET7838449.1"
                     /translation="AAAKVLGNEAIEEVSLGDRAFTKIELGSSYAFKKSVVPILGLDD
                     AALLKLSKDMTLSLNLKELHAIRDYFKKVGREPSDIELESIAQTWSEHCKHKIFAAEI
                     DYSEETGGAPEAQERVDSLYRSCIRATTEELRPRRPDLLSVFTDNAGVFAFNDEYAVC
                     VKAETHNSPSALDPYGGAITGIVGVNRDIIGTGIGAAPLFNTDVLCFAPPSTPDAEVP
                     AGLIPARDLLAGVHRGIVDGGNQSGIPVAAGAFVFDESYLGKPLVFCGTGGIMPLAIR
                     GKPSWEKRIEAGMLAVMVGGRIGKDGIHGATFSSLALDEASPVSAVQIGDPIIQKRMT
                     DFLLEARDLGLYQGITDNGAGGLASSLGEMAESSGGVRIELDACPLKYPGLSPWEILV
                     SESQERMSLAVLPESLEAFMDLARRRGVEATVVGEFVDSGSVEIRAGGKTVGLLGLDF
                     LHRGVPRLHLRAQWRAPAVAEPSLPDRGDWTPVLLELLADPEIASKESLVRQYDHEVK
                     GISIEKPFTGVGRDAPSDGAVLKPLYDDWRGLTVTHGINPRLSDHDAGIMALAAVDEA
                     YRAHIALGGDPERAVALDNFCWPDPVESESNPDGAFKLAQLVRACRSLREACLTYGLP
                     LVSGKDSMKNDAFAGGRRISVKPTLLVTLVGIIDDVRKAPSTDLLAAGELLYLVGLSS
                     PLLGASILERVLARRGGGRLGACPVARPAENLDLYRKLSKAISGKMLRSCHDISDGGL
                     AVALVESCLGGRLGARIRLDPICERGGGGAGIARAAGALFSEEPGRFLVSIRPEDRER
                     FERSVGPAQYLGEVTGEARVEASLAGKSVISADLDALERAFKTAIVQGE"
BASE COUNT          454 a          868 c          892 g          416 t
ORIGIN      
        1 gccgcggcga aggtgctcgg caacgaggcg atcgaagaag tgagcctggg cgaccgggca
       61 ttcaccaaaa tcgagctggg gtcttcctac gccttcaaga agtccgtcgt cccgatcctc
      121 gggctggacg acgccgcgct cctcaagttg agcaaggaca tgaccctgtc gctcaacctg
      181 aaggagttgc acgcgatccg cgactacttc aagaaggtcg ggcgcgaacc cagcgacatc
      241 gagctggagt cgatcgccca gacctggagc gagcactgca agcacaagat cttcgccgcc
      301 gagatcgact acagcgagga gaccggaggc gcgcccgagg cgcaggagag ggtcgactcg
      361 ctctacagga gctgcatccg ggctaccacg gaggagctca ggccccgcag gcccgacctc
      421 ctctcggtgt tcaccgacaa cgcgggcgtc ttcgccttca atgacgagta tgcagtgtgc
      481 gtgaaggccg agacccataa ctcgccctcg gccctggatc cctacggtgg ggccatcacc
      541 ggcatcgtcg gcgtcaaccg cgacatcatc ggtacgggga tcggagcggc tccccttttc
      601 aacacggatg tgctttgttt cgccccaccc tcgacccccg atgcagaggt gccggccggg
      661 ctcatcccgg caagggacct cctcgcgggc gtgcaccgag ggatcgtgga tggaggcaac
      721 caatccggga tacctgtcgc ggccggggca ttcgttttcg acgagagcta tctcggcaag
      781 cccctggtgt tctgcgggac cggcggcatc atgcccttgg cgatcagggg gaagccatcc
      841 tgggagaaga ggatcgaggc cgggatgctg gctgtcatgg tcggcgggcg catcggcaag
      901 gacgggatcc atggagccac cttctccagt ctggccctgg acgaggcgag cccagtgtcg
      961 gccgtgcaga tcggtgatcc gatcatccag aagcgcatga ccgacttcct gctcgaggcg
     1021 cgcgacctgg ggctctacca gggaatcacc gacaacgggg caggcggcct ggcatcgagc
     1081 ctcggcgaga tggcggaatc ctcggggggg gtcaggatcg aactcgatgc ctgccccctg
     1141 aagtaccctg gcctctcacc ctgggagatc ctcgtctcgg agagccagga gcggatgagc
     1201 ctcgccgtgc ttccggaatc gctggaggcc ttcatggacc tcgcgagaag acggggggtc
     1261 gaggccaccg tggtggggga gtttgtggac tcgggctcag tcgagatccg cgctggcggg
     1321 aagacggtcg gcctcctcgg cctcgacttc ctgcaccggg gcgttccaag gctccatctg
     1381 cgggcgcagt ggcgtgcccc cgccgtggcg gagccaagcc ttccggaccg cggggactgg
     1441 acccccgtcc tcctcgagct gctcgcggat cccgagatag cctcgaagga atccctggtc
     1501 aggcagtacg accatgaggt gaaggggata tcgatagaga agcccttcac gggagtggga
     1561 cgcgacgcac ccagcgatgg ggccgtcctc aaacccctct acgatgactg gcgcgggctc
     1621 accgtcacgc acgggataaa cccccggctc tcggaccacg acgccggcat catggcgctc
     1681 gcggcagtgg acgaggccta ccgcgcccac atcgccctcg ggggggatcc cgagcgcgcg
     1741 gtggccctcg acaacttctg ctggcccgat ccggtcgagt ccgaatccaa tcccgacggc
     1801 gccttcaagc tcgcccagct cgtccgcgcc tgccgctccc tgcgcgaggc ctgcctcacc
     1861 tacggcctgc ccctggtctc gggcaaggac tcgatgaaga acgacgcctt cgccggcggg
     1921 cgccgcatct cggtgaagcc gacactcctt gtcacccttg tcggcatcat cgacgacgtg
     1981 cgcaaggcgc cctcgaccga cctgctggcg gccggtgagc tcctctacct cgtcgggctg
     2041 tcctcgccct tgctcggcgc atcgatcctc gagcgcgtcc tcgcccgcag gggagggggc
     2101 cggctcggag cctgcccggt cgcgaggccg gcggagaacc tcgacctgta tcgaaaactc
     2161 tcgaaggcca tctcgggcaa gatgctgcgg tcctgccacg acatctccga tggcggcctc
     2221 gcggtcgcgc tcgtcgagtc ctgtctgggg ggaaggctcg gggccaggat caggcttgac
     2281 cccatctgtg aacgcggggg cggcggggct ggcatagcgc gcgccgcggg agccctgttc
     2341 tcggaggagc ccggacgctt cctggtgtcc ataaggccag aggacaggga gaggttcgag
     2401 cgcagtgtcg ggcccgccca gtacctgggc gaggtgacag gggaggccag ggtcgaggcg
     2461 agcctggccg gaaaatcggt gatcagcgcc gatctcgacg cactggagcg ggccttcaag
     2521 accgccatcg tccaggggga atgagatgga tgtgaaggca atcgtcatca caggatacgg
     2581 cttcaacgcc gacgccgaac tcgcaagggc tttttccctc gccggcgcgg
//
LOCUS       DASSQV010000097         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1768042, whole genome shotgun sequence.
ACCESSION   DASSQV010000097 DASSQV010000000
VERSION     DASSQV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768042"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VFL04_01735"
     CDS             <1..503
                     /locus_tag="VFL04_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET7838450.1"
                     /translation="LAVPIFIGQLETQSILIGMGKVDVPRPLTHDLAISLIHTFGAEI
                     SKIEINDLREGTFFARMVLKAGERDMVVDSRPSDAIGIAVRVDCPVFIAESVVEEAGI
                     SVNLVTGEQTSGEASDKGSAPPLVLQPGDELVRLKTELEEAISEEDYERAAILRDRIR
                     ELEGGL"
     gene            complement(568..>2117)
                     /locus_tag="VFL04_01740"
     CDS             complement(568..>2117)
                     /locus_tag="VFL04_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7838451.1"
                     /translation="MDKKTGAPSLSNYAQFFGRKYYINAFAHSMVIAVATTAFSILIG
                     VPFAFLTTRYRIRGSTLLNTLAVLALLSPPFIGAYSWVTMLGRSGFLRQALMGMGLAL
                     PPIYGPIGIVLADTLQYYPFVTLMTAGALMTTDRSLEEASQNLGARGLRTFLTVTMPL
                     VLPSLTGGALIVFMMSLSNFGTPMIIGGNYLVLPTLAYNLYTSEVAESPGMASTVSIL
                     LMVCASGVIALQQWASSRRKYASMLVNRPVKKRLGSAWTVPAHALCYLIVALSTLPLA
                     VVVYYSFRNTNGPVFASGYGLASYRAIFFDVPRTVTNSFLYAFAAVLLIASVGTLLGF
                     VIARRRRLSVRILDPLLMVPYMVPGTVLGIGFIVAFNRKPLYLAGTGVLIILTYFIRR
                     LPYSVRSAASILKQIDPALEEAGVNLGSPPGRTFRTIALPLMKAGIVSGAIMSWVTSM
                     NELSASILLYVGRTMTMPIKIYLSVLDGYFGTASAMSTILLLVTGISLYLVNRLTGKS
                     GESIVAL"
BASE COUNT          402 a          620 c          782 g          313 t
ORIGIN      
        1 acctggcggt ccccatcttc atcggccagc tcgagaccca gtcgatcctc atcggcatgg
       61 gcaaggtcga cgtgcccagg cccctcaccc acgacctcgc catctccctc atccacacct
      121 tcggggccga gatctccaag atcgagatca acgacctgcg cgagggcacc ttcttcgcgc
      181 gcatggtcct gaaggcgggg gaacgggaca tggtcgtgga ctcgaggccc tcggacgcca
      241 taggcatcgc cgtgcgcgtc gactgccccg tcttcatcgc cgagtccgtc gtcgaggagg
      301 ccgggatctc ggtgaacctc gtcacggggg agcagacgtc tggcgaggcc tccgacaagg
      361 gctcggcccc gcccctcgtc ctccagccgg gagacgagct cgtcaggctc aagaccgagc
      421 tcgaggaggc gatctccgag gaggactacg agagggcggc catcctcagg gacaggatca
      481 gggagctcga gggcggactc tagaggcccc caggcgggct cgagggaacc cgcccccccc
      541 ccccgatgcc acgcgccgct ggcgcgtcta cagcgcgacg atcgactcgc ccgatttccc
      601 cgtgaggcgg ttaacgaggt agagcgagat ccccgtcacg aggaggagga tcgtcgacat
      661 cgccgaggcc gtcccgaagt acccgtccag caccgaaaga tagatcttga tgggcatcgt
      721 catcgtcctg ccgacgtaca ggaggatgga ggccgagagc tcgttcatcg aggtcaccca
      781 gctcatgatc gcgcccgaca cgatgcccgc tttcatgagg gggagggcga tggtcctgaa
      841 ggtgcgtcct ggcggcgagc cgaggttgac cccagcctcc tcgagggccg ggtcgatctg
      901 cttgaggatc gaggccgccg acctcaccga gtagggcagg cgccgtatga aataggtgag
      961 gatgatgagg accccggtgc cggcgaggta caggggcttg cggttgaagg cgacgatgaa
     1021 gccgatgccg agcacggtgc cgggcaccat gtagggcacc atgaggaggg ggtcgaggat
     1081 cctgaccgag agccgtcggc gcctggctat gacgaagccg aggagggtgc cgaccgatgc
     1141 gatgagcagg accgcggcga aggcgtagag gaaggagttc gtaacggtcc tggggacgtc
     1201 gaagaagatc gcgcggtagc tcgcgaggcc gtagcccgag gcaaacaccg gcccgttcgt
     1261 gttgcggaag gagtagtaga ccacgacagc gaggggcagg gtgctcaggg ccacgatgag
     1321 gtagcagagg gcgtgggcgg ggaccgtcca tgccgagccg aggcgcttct tgacgggtcg
     1381 gttcacgagc atgctcgcgt atttcctccg cgaggaggcc cactgctgca gggcgatgac
     1441 gcccgaggcg cagaccatga ggaggatgct caccgtgctc gccatgccgg ggctttccgc
     1501 gacctcgctc gtatagaggt tgtaggcgag ggtggggagg acgaggtagt tgccgccgat
     1561 gatcatcggc gtgccgaagt tcgagaggga catcatgaac acgatgaggg ccccgcccgt
     1621 gagcgaaggc aggacgaggg gcatggtcac cgtgaggaag gtcctgaggc cccgggctcc
     1681 gaggttctgc gaggcctcct cgagcgagcg gtccgtggtc atgagggcgc ccgccgtcat
     1741 gagggtgacg aaggggtagt actgcagggt gtccgcgagg acgatgccga tggggccgta
     1801 gatcggcgga agggcaaggc ccatgcccat cagggcctgg cgcaggaagc ccgagcggcc
     1861 gagcatggtg acccaggaat acgccccgat gaagggtggc gagaggaggg cgagaacggc
     1921 cagggtgttg agcagggtcg agcccctgat ccggtagcgc gtggtcagga aggcgaaggg
     1981 cacgccgatg aggatggaga aggccgtcgt ggcgacggcg atgaccatgc tgtgcgcgaa
     2041 ggcgttgatg tagtacttcc gcccgaagaa ctgggcgtag ttgctcaatg agggggcgcc
     2101 ggtcttcttg tccatga
//
LOCUS       DASSQV010000098         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_523641, whole genome shotgun sequence.
ACCESSION   DASSQV010000098 DASSQV010000000
VERSION     DASSQV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523641"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1875)
                     /locus_tag="VFL04_01745"
     CDS             complement(<1..1875)
                     /locus_tag="VFL04_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HET7838452.1"
                     /translation="MPKRGETMIGSSLARADGPDKVRGRTRFADDERRPGLLHAVVLP
                     SSQAHARILSVATAEAKAAPGVRGVFTGADWTQLIGLYLGDKPPLARGIVRHQGEGVA
                     AVVADDEASARRALGLIRVEYEGLPVLNDPRSALAPEAPLIHPDMASYRHIPAIMPEP
                     GSNVAHRSRIRKGEPEAAMAAAPVRIRGEYSFPPGDHCAMEPRSAQAEVLADGRVLIR
                     SSTQSPFGVRTLMSQGFGIPQGQIEVTAPAVGGGFGGKAGIQLEPLAYLLSRALGGRR
                     VRLANDREGDMLGSPGRPGIQARVELGAREDGVILAADIEFLFDSGGYADYAVNVARA
                     AGYACTGPYRVPNVKADSLCVYTNHPFATAFRGFGHIEMSFCVERAMDALAARLGMDP
                     MRLRLINAVGPGDTTPSGSVLEPCTGELPACIEKVAGRIGWTEGGPASVPSGAGRYRA
                     KGISCFWKAPAIPTFTDAGAILLFNDDGSVNLRTGVVELGQGALTGLSQIVAEALGIE
                     ASRVHVNPEVSTASSPHDWTTAASRSLFMAGRAALAAARDAADQIKAIASRPLRCPEE
                     DLEVGGGRVYLRDDPERGLGLEKVVLGYSYPDGSSIGGPVIGRGHYIARGLTNLDPET
                     G"
BASE COUNT          269 a          720 c          771 g          291 t
ORIGIN      
        1 gcccgtctcg ggatcgaggt tggtaaggcc cctcgcgatg tagtggcccc tgccgatgac
       61 cgggccgccg atcgaggagc cgtcgggata gctgtagccg aggaccacct tctcgagccc
      121 gaggccccgc tcgggatcgt cccgcaggta gaccctgccc cctcccacct cgaggtcctc
      181 ctcgggacag cgcaggggcc gcgaggcgat ggccttgatc tggtcggcgg cgtccctggc
      241 cgcggcgagg gccgcccgcc ccgccatgaa gagcgagcgc gatgccgccg tcgtccagtc
      301 gtggggcgag gaggccgtcg atacctcggg attcacgtgg accctggagg cctcgatgcc
      361 gagggcctcg gcgacgatct ggctcaggcc tgtgagggcc ccctggccga gctcgacgac
      421 cccggtccgg aggttgacgc tcccgtcgtc gttgaagagg aggatcgccc cggcgtcggt
      481 gaaggtgggt atggcggggg ccttccagaa acaggagatg cccttggccc tgtacctgcc
      541 cgcgccggag gggacagagg cgggcccgcc ctccgtccag ccgatccggc cggccacctt
      601 ctcgatgcag gcggggagct cgcccgtgca gggctcgagc accgagcccg agggcgtggt
      661 atcgccaggc cccacggcgt tgatgagccg gagccgcatc gggtccatgc cgagcctggc
      721 ggccagggcg tccatcgccc gctcgacgca gaaggacatc tcgatgtggc cgaagcccct
      781 gaaggccgtc gcgaagggat ggttcgtgta cacgcagagc gagtcagcct tgacgttggg
      841 gacgcggtag ggccccgtgc aggcgtagcc tgctgccctg gcgacgttga cggcgtagtc
      901 ggcgtagccg cccgagtcga agaggaactc gatgtcggcg gcgaggatga ccccgtcctc
      961 cctcgcgccg agctcgaccc tggcctggat ccccggcctt ccgggcgagc cgagcatgtc
     1021 gccctcgcgg tcgttcgcga gcctcacccg cctgccgccg agggcgcgcg agagcaggta
     1081 ggcgaggggc tcgagctgga tgccggcctt gcccccgaag cccccgccca cggccggcgc
     1141 cgtcacctcg atctggccct gggggatgcc gaagccctgg ctcatcaggg tcctcacgcc
     1201 gaagggggac tgggtcgagg agcggatgag gaccctgccg tcggcgagga cctcggcctg
     1261 ggccgagcgc ggctccatcg cgcagtggtc cccggggggg aaggagtact cgccccgtat
     1321 cctcaccggg gcggcggcca tggcggcctc gggttcgccc ttcctgatcc ggctccgatg
     1381 cgcgacgttg ctgccgggct cgggcatgat cgcggggatg tggcggtagg aggccatgtc
     1441 ggggtggatg agtggggcct ccggggccag ggccgagcgg gggtcgttga gcaccggcag
     1501 accctcgtac tcgacccgta tgaggcccag ggcgcggcgg gcgctcgcct cgtcgtcggc
     1561 gacgacggcc gcgaccccct cgccctggtg acgcacgatc ccgcgcgcga gggggggctt
     1621 gtcgccgagg tagagcccga tgagctgggt ccagtcggcc ccggtgaaga cccccctcac
     1681 cccgggggcg gccttcgcct cggcggtcgc cacggagagg atccgggcgt gggcctggct
     1741 gctcgggagg accacagcat ggaggaggcc gggacgcctc tcgtcgtcgg cgaacctcgt
     1801 cctgcccctg accttgtcgg gaccgtcggc tcgggccagg gaggagccga tcatcgtctc
     1861 gccgcgcttt ggcatgggaa gcagtctagc gccgcggccc tgcaggcgca accgaggccc
     1921 gccgccccgg ccccggctgg gcggccacgc ccgggctggt gcctcgagcc ctcctggccc
     1981 cgcccaagcc ccccgccctg gggcgggtct tttgtccctt gccggcggga ccgcgaggag
     2041 ttaaaataaa g
//
LOCUS       DASSQV010000099         3568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1393580, whole genome shotgun sequence.
ACCESSION   DASSQV010000099 DASSQV010000000
VERSION     DASSQV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393580"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFL04_01750"
     CDS             complement(<1..312)
                     /locus_tag="VFL04_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838453.1"
                     /translation="MLACLLLASSCARSAAPVAPDSLPKPDGGAMMETVKALASTDNA
                     RVAGTEGEEGAAAYLAERFRSLGLEVSIDEFPILAFRTTSASLGLGSGTTLRADALLY
                     SP"
     gene            complement(368..571)
                     /gene="thiS"
                     /locus_tag="VFL04_01755"
     CDS             complement(368..571)
                     /gene="thiS"
                     /locus_tag="VFL04_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET7838454.1"
                     /translation="MTITLNFESEHYEEASLSVLDILKRKHWSFPLVIVKVNGEVIER
                     TAYGECAVKDGDEVEAYHLVSGG"
     gene            649..2556
                     /locus_tag="VFL04_01760"
     CDS             649..2556
                     /locus_tag="VFL04_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET7838455.1"
                     /translation="MPRSGLGRFVSWLFGVGGMKRTILIPYVCLIVTGFGAGGFLYLQ
                     GSRSAVLGAVDLLLAESAARVSDRLEEFLAGSVALAEANASQVAEIPDSSAGLQRLRR
                     LFFRELQERDDVDLASVGYADGRYVEAQRVTGGAIHVGEADASAGRSLLLQSADSHGD
                     PLAVLQERPGYDPRTRPWYLKAAEAERTAWTEPYPIVSSNELSMTAVSPIYRDGRLAA
                     VTTANVTLGRLSTYLAKMETSRSGIAAIADKGGRLLASSDFSSLVDQGGLRRMAGDGE
                     GVAALTFREAAKRAPGSPFSLNDGQLRYRAVARDWSGPWGLSWRVVVALPESMFYAPL
                     EAIDHRALVILVVMLALVLVIAFFAAGRVSGPLRELGKAISMLEPDSPSLHPAFPSDT
                     GVERARLIAHRSDEIGQLASTFVMMSGRLTDGIKVLKASLAEKEILLKEVHHRVKNNL
                     QIVSSILSLQAGLSEDEAFRESIEYMQDRIQAMAFVHEDLYRSGDFRAVGMAAYLGRV
                     CDSLALADHLGKATRIIVEADGIELPLERALPCGLLVNELVTNALKHAFAGKPSGRVR
                     VSLSRSGDMYILVVSDDGVGMSQSSNRLGEAGREGLGSQLVPGLADQLKGRLEIETGQ
                     EGTTARISFPV"
     gene            2787..3191
                     /locus_tag="VFL04_01765"
     CDS             2787..3191
                     /locus_tag="VFL04_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7838456.1"
                     /translation="MIKYLKTNDEKCVGCMTCTAACSKLYFKEENPAKSAIQVNGLGK
                     GAFHLIACDQECRKCVAECPTQAISVSKSGVVMIDRKLCVGCLACVAVCPTEAMRYYP
                     GHKNPFKCVACGACVKTCPKQALEIVQKEESV"
     gene            3188..>3568
                     /locus_tag="VFL04_01770"
     CDS             3188..>3568
                     /locus_tag="VFL04_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET7838457.1"
                     /translation="MSPSKKDFPLLSEWRYEKGQVHRGYNMRTLHVDVGSRVAKEKPV
                     SEDMKRRFVGGKGFGLKLLWDGTKPSTKWDDPENEIVIAMGPVCGNTNYPGSGKSLVV
                     SISPMTGVPIDSNVGGYFGPYLKFS"
BASE COUNT          609 a         1220 c         1160 g          579 t
ORIGIN      
        1 cggggaatac aggagggcgt cggccctgag cgtcgtgccc gagccgaggc ccaggctggc
       61 cgacgtggtc ctgaaggcca ggatggggaa ctcgtctatg ctgacctcga ggcccaggga
      121 gcggaaacgc tcggcaaggt aggcggctgc cccttcctcg ccctcggtgc cggccacgcg
      181 ggcgttgtcg gtcgaggcga gggccttcac cgtctccatc atcgccccgc cgtcgggctt
      241 cgggagggag tccggcgcga cgggagcagc gctccgcgcg catgaggagg caaggaggag
      301 acaggcgagc atcagggcgc cgccaagccg cggctggctg ggggaggccc gatgccgcgg
      361 ccggccctca gccccccgag accagatggt aggcttcaac ctcgtctccg tcctttacgg
      421 cgcactcgcc gtaggccgtc ctttcgatca cctcgccgtt caccttcacg atgaccagag
      481 ggaaggacca gtgcttgcgc ttgaggatgt cgaggacgct gaggctcgct tcctcgtaat
      541 gctccgactc gaagttgagg gtaatcgtca ccccccgagc ttagcttccc cgccgaagtg
      601 cgacaagctg ccttggccgc ggggcccggc ctgctgtact atccccgaat gccgcggtcc
      661 ggcctcggcc gtttcgtctc ctggctcttc ggggtcggcg ggatgaagcg gaccatcctc
      721 ataccctatg tctgcctcat cgtcacgggt ttcggggccg ggggcttcct ctacctccaa
      781 ggctcccgca gcgcagtgct cggggcagtc gacctcctcc tggcggaatc ggcggcccgg
      841 gtcagcgaca ggctcgagga attcctcgct gggtcggtgg ccctcgccga ggccaacgcc
      901 tcccaggtgg ccgagatccc cgattcgagc gccggcctcc agcggctcag acgcctcttt
      961 ttcagggaac tccaggagag ggatgacgtc gacctggcga gcgtcggcta cgccgacggg
     1021 cgctacgtcg aggcccagag ggtgaccgga ggggcgattc atgtcggcga ggccgacgcc
     1081 tcggcaggga ggagccttct cctccagagc gccgacagcc acggcgaccc cctcgccgtg
     1141 ctccaggaga ggccgggcta cgatcccagg accaggccct ggtacctcaa ggcggccgag
     1201 gccgaaagga cggcctggac cgagccctat cccatcgtct cgtccaacga gctcagcatg
     1261 actgccgtca gcccgatcta ccgcgatggc aggctcgcgg ccgtgacgac ggccaacgtc
     1321 accctcggcc gcctgtccac ctatcttgcg aagatggaga cctcgaggtc gggcatcgcc
     1381 gccatcgccg acaagggcgg gcgcctcctg gccagctcgg atttctcctc cctcgtggat
     1441 cagggaggcc tgcgccggat ggccggggac ggcgaggggg tggccgccct caccttccgg
     1501 gaggcggcga agcgcgcccc gggctccccc ttctcgctca atgacggcca gctccgctac
     1561 cgggccgtcg ccagggactg gtcggggccc tggggcctct cctggcgggt tgtcgtggcc
     1621 ctccccgaat ccatgttcta cgcccccctc gaggcgatag accaccgcgc cctcgtcatc
     1681 ctcgtggtca tgctggcctt agtcctggtg atagccttct tcgcggccgg acgggtctcc
     1741 ggccccctgc gcgagctggg caaggccatc tcgatgctcg agccggattc gccaagcctc
     1801 caccccgcct tcccctccga cactggggtc gagagggccc ggctcatcgc tcacaggtcg
     1861 gacgagatcg gccagctcgc atccaccttc gtcatgatga gcgggaggct caccgatggc
     1921 atcaaggtcc tcaaggcctc gctggcggaa aaggagatcc tgctcaagga ggtgcatcat
     1981 cgagtcaaga acaacctcca gatcgtctcg agcatcctga gcctccaggc cggcctctcc
     2041 gaggatgagg ccttccggga atcgatcgag tatatgcagg acaggatcca ggccatggcc
     2101 ttcgtccatg aggacctcta ccgctcggga gatttccggg ctgtggggat ggccgcctac
     2161 cttgggaggg tctgcgactc cctggccctg gccgaccacc ttggcaaggc cacgaggata
     2221 atcgttgaag ccgatggcat tgagctcccc ctcgagcgcg cccttccctg tggcctcctc
     2281 gtcaacgagc tggtcacgaa tgccctgaag cacgccttcg cggggaagcc cagcggcagg
     2341 gtcagggtct ccctgtcgag atccggggac atgtacatcc tggtggtctc ggatgacggg
     2401 gtcgggatgt cccagtcctc aaaccggctg ggggaggcgg ggcgcgaagg ccttggctcc
     2461 caactcgtgc ccggcctcgc cgaccagctc aagggcaggc tcgagatcga gacgggccag
     2521 gaaggcacga cggcgaggat cagcttcccg gtctgatggg ctcaccttcg gaggcttcag
     2581 cttccgattg cggaaaactg gaatctcggc tatactgata tatcagtcca ctcatcccca
     2641 tgccgcagca aagcgaagct ggcgccccag ggtgtccaga ggggtgaaac cggtcccggt
     2701 gtcccgctac gacccggcag tgaaccagtg gggcccgacc gccggaacag cgcggtagtg
     2761 aggcccggca acaggaggtc tccgccgtga tcaagtatct caagacgaac gacgagaaat
     2821 gcgtcggctg catgacctgc acggccgcat gctcgaagct ctacttcaaa gaggaaaacc
     2881 ccgccaagtc ggcgatccag gtcaacggcc tgggcaaggg cgccttccac ctcatcgcct
     2941 gcgaccagga atgccggaag tgcgtcgccg aatgccccac ccaggccatc agcgtcagca
     3001 agtcaggcgt cgtcatgatc gacaggaagc tctgcgtggg ctgtctcgcc tgcgtcgccg
     3061 tctgtcccac cgaggcgatg cgctactacc cggggcacaa gaaccccttc aagtgcgtcg
     3121 cctgcggcgc ctgcgtgaag acctgcccga agcaggccct ggagatcgtt cagaaggagg
     3181 agagcgtatg agccccagca agaaggactt ccccctcctg tccgagtggc gctacgagaa
     3241 gggccaggtc cacaggggct acaacatgag gaccctccac gtcgacgtgg gctcccgggt
     3301 cgcgaaggag aagcccgtct ccgaggacat gaagcggcgc ttcgtcgggg gcaagggctt
     3361 cggcctcaag ctgctctggg acggcaccaa gccctccacg aaatgggacg accccgagaa
     3421 cgagatcgtc atcgccatgg gccccgtctg cggcaacacg aactaccccg gctcgggcaa
     3481 gagcctcgtc gtgtccatct ccccgatgac cggggtgccc atcgactcca acgtcggcgg
     3541 ctacttcggg ccctacctca agttctcg
//
LOCUS       DASSQV010000100         3378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_275413, whole genome shotgun sequence.
ACCESSION   DASSQV010000100 DASSQV010000000
VERSION     DASSQV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275413"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="VFL04_01775"
     CDS             <1..1713
                     /locus_tag="VFL04_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HET7838458.1"
                     /translation="RNAATIADNVGDNVGDCAGMAADIFESYEVTIVAAMILGLATFG
                     HIGVMFPLLVRGIGVLGSIISTYTVRAKADDTSDVALKNVHRGFWIGSAISVVGFMVL
                     GFFYLRHAPMEGLSNPWWANFGLEGLDMRPALTCFIGVVLAIVLNKVTSYYTHTSHEP
                     VKSLTKACQTGHATNIIQGFAVGYESTVVNIIVIAVAIAISVLVYAGTNPLFVAYGVA
                     MTGIGMLTLTGNTISMDVFGPVADNANGIGEMGYDKAEMEKEKPGSYKRARQILADLD
                     AVGNTTKAETKGIAIGSAVIAAVSLFASFIAVIAMGSEDKIGLLGSNMNLYKAIEAQL
                     SVASPMVMIGLLIGGAVPFLFSSMLIRAVGRAAYLIVKECRLQFQDKEIWGGHKKPDY
                     ARVVDICTRTAQNELIGPGFLAILSPILVGFLLGPFALGGFLAGSIVVGQLLAVFMAN
                     AGGAWDNAKKMIEDGLYGGKGSDAHKASVTGDTVGDPLKDTAGPAINPLIKVMNMVSL
                     LVLVIVMNFGFVRIDPSATASYVLAGVVVAVCLGVLAWAVWQSKRVTPEMLEVERCLE
                     EQGV"
     gene            complement(1830..>3378)
                     /locus_tag="VFL04_01780"
     CDS             complement(1830..>3378)
                     /locus_tag="VFL04_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET7838459.1"
                     /translation="SMEKTAPTLLLVEDEAIIAMAETKELSDRGFRVVHVPTGERAVE
                     TMRTGTLAIDLVLMDIDLGRGMDGTEAAREILKASEVPIVFLSSHTERSIVERTETIA
                     SYGYVLKSSGVTILTAAIRMAFQLHAAHQENKDKSRQLEHSNSRLCDTVEELQVASEE
                     LSTANDSLLAACADLQRSEESLKASERRYHSLFRNMLDGFAYCAIHRDSEGRPCDWTY
                     LETNEAFERLTGLRDATGRRVSELIPGILEADPELFEIYGRVAATGAPERFEMEVCSL
                     GQWFAVSVYSPEPGHFIAVFEVITKRKLTEQALERSLSEKEVILLELRHRVKNNLGII
                     SGLLAMGASKAGDSEAGRLLEDARSRVDAMSGIYEALIQTDQIDLVEMPQYLGRLASS
                     LYETSVIEKDRISISVDIDVCALELRRAVPLCLIVNELFSNALKYAYPAPRRGSIDLA
                     LRAQDGELSVTVSDRGVGLPQGLDPKTCASTGLSLVRMLAGQLGAALDFECVPGGGTR
                     ARVVLRA"
BASE COUNT          530 a         1197 c         1053 g          598 t
ORIGIN      
        1 cgcaacgcgg ccacgatcgc cgacaacgtc ggcgacaacg tcggcgactg cgccggcatg
       61 gcggccgaca tcttcgagtc ctacgaggtc acgatcgtcg cggccatgat cctgggcctg
      121 gccaccttcg gccacatcgg cgtcatgttc ccgctgctcg tgcgcggcat cggcgtcctc
      181 ggctcgatca tctccaccta cacggtcagg gccaaggccg acgacacctc cgacgtcgcc
      241 ctcaagaacg tccaccgcgg cttctggatc ggctcggcca tctcggtggt cggcttcatg
      301 gtcctcggct tcttctatct ccgccacgcc ccgatggaag gcctctccaa cccctggtgg
      361 gccaacttcg ggcttgaggg cctcgacatg cgaccggccc tcacctgctt catcggcgtc
      421 gtgctggcca tcgtcctgaa caaggtgacg agctactaca cccacacgag ccacgagcct
      481 gtgaagagcc tcaccaaggc ctgccagacc ggccacgcca ccaacatcat ccagggcttc
      541 gcggtcggct acgagtccac cgtcgtcaac atcatcgtca tcgcggtcgc catcgcgatc
      601 tccgtcctcg tctacgccgg cacgaacccc ctgttcgtcg cctacggcgt cgccatgacg
      661 ggcatcggca tgctcaccct caccggcaac acgatctcca tggacgtctt cggaccggtc
      721 gccgacaacg cgaacggcat cggggagatg ggctacgaca aggccgagat ggagaaggag
      781 aagcccggca gctacaagcg cgcccggcag atcctcgccg acctcgacgc cgtgggcaac
      841 accacgaagg ccgagaccaa gggcatcgcg atcggctcgg ccgtcatcgc ggccgtctcc
      901 ctcttcgcct ccttcatcgc ggtcatcgcg atgggcagcg aggacaagat cggcctcctc
      961 ggctcgaaca tgaacctcta caaggccatc gaggcccagc tctcggtcgc gagccccatg
     1021 gtcatgatcg gcctcctcat cggcggggcc gtgcccttcc tcttcagctc catgctgatc
     1081 agggccgtcg gccgcgccgc ctacctcatc gtcaaggagt gccgcctcca gttccaggac
     1141 aaggagatct ggggcgggca caagaagccc gactacgccc gcgtcgtcga catctgcacc
     1201 cgcacggcgc agaacgagct catcgggccc ggcttcctcg ccatcctctc gcccatcctc
     1261 gtcggcttcc tcctcgggcc cttcgccctg ggcggcttcc tcgccggctc catcgtcgtc
     1321 ggccagctcc tggccgtctt catggccaac gcgggcggag cctgggacaa cgccaagaag
     1381 atgatcgagg acggcctgta cggcggcaag ggctcagacg cccacaaggc ctcggtcacc
     1441 ggcgacaccg tcggcgaccc cctcaaggac acggccggcc ccgcgatcaa ccccctcatc
     1501 aaggtcatga acatggtgag cctcctcgtc ctcgtcatcg tcatgaactt cggcttcgtc
     1561 cggatcgacc cgagcgccac agcgagctat gtcctcgccg gcgtggtcgt ggcggtctgc
     1621 ctcggcgtcc tcgcctgggc ggtctggcag tccaagcgcg tgacccccga gatgctcgag
     1681 gtcgagcgct gtctcgagga gcagggagtc tgatcccggc ctgagacagg cccgaaagca
     1741 tcctgcgaaa tgaaggcggg gcccctgggg gccccgcctt ttttcttgcc cctggacccg
     1801 cggtccagcg ggcccttccc tggcagccct caggccctga ggaccactct ggcgcgggtg
     1861 cccccgcccg ggacgcactc gaagtccagg gccgccccca gctggcccgc cagcatgcgc
     1921 acgaggctga ggccggtgct ggcgcaggtc ttcggatcga ggccctgggg caggccgacc
     1981 cccctgtcgc tcaccgtcac ggagagctcc ccgtcctggg ccctgagcgc gaggtcgatg
     2041 gagccgcggc ggggggcggg gtaggcgtat ttgagggcgt tggagaacag ctcgttgacg
     2101 atcaggcaga gggggacagc gcgccggagc tccagtgcgc agacgtcgat gtcgacgctg
     2161 atgctgatcc tgtccttttc gatcacggag gtctcgtaga gcgagctcgc caaacggccg
     2221 aggtactgcg gcatctcgac aaggtctatc tggtcggtct ggatgagggc ctcgtagatc
     2281 ccgctcatgg cgtcgaccct ggagcgagcg tcctcaagca ggcgcccggc ctcactgtcc
     2341 cctgccttgc tcgcgcccat cgccaggagg cccgagatga tgccgaggtt gttcttcacg
     2401 cggtgcctga gctcgaggag gatgacctcc ttctcggaga gcgagcgctc gagggcctgc
     2461 tctgtcagct tccgctttgt gatgacctcg aacaccgcga tgaaatggcc gggctcgggg
     2521 ctgtagaccg agaccgcgaa ccactgcccg agggagcaga cctccatctc gaagcgctcc
     2581 ggagcccccg tggccgccac cctcccgtag atctcgaaga gctcggggtc ggcctcgagg
     2641 atcccgggga tcagctcgct cacccttctg cctgtggcgt ccctgaggcc ggtgagccgc
     2701 tcgaaggcct cgttcgtctc gagataggtc cagtcgcagg gtctgccctc gctgtccctg
     2761 tggatcgcgc agtatgcgaa gccgtcgagc atgttgcgga agagcgagtg atagcgccgc
     2821 tcgctggcct tcagcgattc ctcgctcctc tgcaggtcgg cgcaggcggc caagaggctg
     2881 tcgttcgccg tgctgagctc ctcgctggcg acctgaagct cctcgacggt gtcgcagagc
     2941 ctggaattgc tgtgctcgag ctgacggctc ttatccttgt tctcctggtg ggcggcatgg
     3001 agctggaagg ccatcctgat cgccgccgtg aggatcgtga cccccgagct tttgaggacg
     3061 tagccgtagg acgctatcgt ctcggtgcgc tcgacgatgc tgcgctcggt gtgcgaggag
     3121 aggaagacga tcgggacctc gctcgccttc aggatctcgc gcgctgcctc ggttccgtcc
     3181 attcccctgc cgaggtcgat gtccatgagg acgaggtcga tggccagggt ccccgtcctc
     3241 atggtctcga cggcccgctc gcccgtgggc acgtggacca ccctgaaacc cctgtcagac
     3301 agctccttcg tctccgccat cgcgatgatc gcctcgtcct cgacgaggag gagtgtcggc
     3361 gccgtttttt ccatgctc
//
LOCUS       DASSQV010000101         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2016783, whole genome shotgun sequence.
ACCESSION   DASSQV010000101 DASSQV010000000
VERSION     DASSQV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2016783"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFL04_01785"
     CDS             complement(<1..761)
                     /locus_tag="VFL04_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838460.1"
                     /translation="MKRRRTSLLALAAASLALAAPLTAIEIGVEPHAGNVMIPWSQDT
                     PTLGAQFPATNYFFGGSAWFTAPLGEDASIRVTYDRDPVLRNTVVAAVLFERGVAKIA
                     VGPLVGLYNTAALPLSAGLSASVTLRWPGTAYVAIRSDGGLALSVLQTGTDPQARTEL
                     SAGFYVPNAIVSGVLVAKRFNETDANGNLKATDSFTRYALTVDIFKKNVPYTVLGSIG
                     YELRSKKYVASAVTDTLGSVVAGADVSAQIAEGIRL"
     gene            complement(772..2007)
                     /locus_tag="VFL04_01790"
     CDS             complement(772..2007)
                     /locus_tag="VFL04_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838461.1"
                     /translation="MTPKSSVALSILCLAAALCPISVRAADLSVDTLDLVTHGGLNET
                     TGHFEMSTRLFFDLSMRGGDKFAGLLRLEFQNSNVEKAISLGGNQAPSAPVAYDQNQL
                     QQVIDKLNNLTSPTIRTAAVTAREAFDLPLDLTYFVGFLDTFGSGDDFGPLFGAPPFG
                     TKLRGPMVYPNGVGGDPNLYYDGIAAANGTGFRLGTTPKLSDSMVAYLYMYQDSDIGS
                     GYWSADGRILLNGDHLKFEIFTGASTFGGAKLGVYRGGFLFYAAPGEVGEFFAQVGVP
                     HWDPTTSFSVDSLFFLFEPRVNFGAGNLALTVFYHPSWYRERDNSNPLSGEKGALDTA
                     VNLGFGRLDQTGVLGGLEGLLEFRPLTTTPLTVLVSPYYSAISGGVEWDFKLGLKLFP
                     LPSQWYGVLEPFIGLKTSF"
BASE COUNT          375 a          643 c          816 g          363 t
ORIGIN      
        1 agcctgatcc cctcggctat ctgggcgctc acgtcggccc cggcgacgac cgagcccagg
       61 gtgtcggtga cggcgctcgc gacgtacttc ttggagcgca gctcgtagcc gatcgacccg
      121 agcacggtgt aggggacgtt ctttttgaag atgtcgacgg tgagcgcata acgcgtgaag
      181 gagtcggtcg ccttcaggtt gccgttcgcg tccgtctcgt tgaagcgctt ggcgacgagg
      241 acccccgaga cgatggcgtt cggcacgtag aagcccgccg agagctcggt ccgcgcctgg
      301 gggtcggtgc cggtctggag gaccgagagg gcgaggcccc cgtccgatcg gatcgcgacg
      361 taggccgtgc ccggccagcg cagggtgacc gaggccgaga ggcccgccga caatggcagg
      421 gcggcggtgt tgtagagccc gacaaggggg ccgacggcga tcttggccac ccccctctcg
      481 aagaggacgg cggcgacgac ggtgttgcgg agcacgggat cgcggtcgta ggtgacgcgg
      541 atggaggcgt cctcgccgag gggggccgtg aaccaggccg agcccccgaa gaagtagttg
      601 gtcgccggga actgggcgcc cagggtcggc gtgtcctggc tccaggggat catcacattg
      661 cccgcatggg gctccacccc gatctcgatg gccgttaggg gggcggccag ggcaaggctg
      721 gccgcggcca gggcgagcag gctcgttcgt cgtcttttca tggggcggcc ttcagaatga
      781 ggtcttgagc ccgatgaagg gctcgaggac gccgtaccac tggctgggca gggggaagag
      841 cttgaggccc agcttgaaat cccactcgac cccgcccgag atcgccgagt aataaggaga
      901 taccaggacg gtgagggggg tggtcgtgag cggcctgaac tcgaggaggc cctcgaggcc
      961 tccaagcacc ccggtctggt caagccggcc gaagccgagg ttgaccgccg tgtcgagggc
     1021 tcccttctcg cccgagaggg gattgctgtt gtcgcgctcg cggtaccagg aggggtggta
     1081 gaacacggtg agggccaggt tcccggcgcc gaaattgacc cggggctcga agaggaagaa
     1141 gagcgagtcg actgagaagc tcgtggtggg atcccagtgg ggcaccccga cctgggcgaa
     1201 gaattccccc acttccccgg gggcggcgta gaagaggaag ccgccccggt atacgccgag
     1261 ctttgctccc ccgaaggtcg aggcccccgt gaagatctcg aacttgaggt ggtcgccgtt
     1321 gaggaggatg cggccgtcgg ccgaccagta gcccgagcct atgtccgagt cctggtacat
     1381 gtagaggtag gcgaccatcg agtcggacag cttcggcgtg gtcccgaggc ggaatcccgt
     1441 gccgttggcg gcggcgatgc cgtcgtagta gaggttgggg tccccgccca ccccgttggg
     1501 gtagaccatg ggcccgcgca gcttggtccc gaatggcggc gccccgaaga gggggccgaa
     1561 atcgtcgccc gagccgaagg tgtcgaggaa gccgacgaag taggtgaggt ccaggggcag
     1621 gtcgaaggcc tcgcgggcag tgacggcggc ggtgcggatc gtggggctcg tcaggttgtt
     1681 gagcttgtcg atcacctgct ggagctggtt ctgatcgtac gcgacagggg cggagggagc
     1741 ctggttgccc cccagactga tggccttctc gacgttgctg ttctggaact cgagccggag
     1801 gaggccggcg aacttgtccc cgccgcgcat cgagaggtcg aagaagagcc tcgtgctcat
     1861 ctcgaagtgg cccgtggtct cgttcagccc gccatgggtc acgaggtcga gggtgtcgac
     1921 gctgaggtcg gcggctcgga ccgatatagg gcaaagggca gccgcaaggc agagtatcga
     1981 tagcgcgacg gagctcttcg gcgtcatgct ctctcctgga agacggggcc gtaatcgtaa
     2041 ttttagcgcc gatccgcgcg gaatgcacgg cgccatatga ttatcggccg gaaacacggg
     2101 ctgtcgagcc gaaagctctg ctccggccct tgtcccggcc cggcagggtc catgttgaaa
     2161 ggctcgtggc cttgacgtat cgcctcatct atataat
//
LOCUS       DASSQV010000102         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_275844, whole genome shotgun sequence.
ACCESSION   DASSQV010000102 DASSQV010000000
VERSION     DASSQV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275844"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="VFL04_01795"
     CDS             complement(<1..1513)
                     /locus_tag="VFL04_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7838462.1"
                     /translation="MGFVMDGLESEAYDRQYSDRQLLARIGSYFRPFRRRIALVGCVL
                     AAASLADFGAQVVVAKSLDAAAKRPEVWVFALIGLAVIGFGAAEWLFNYLRYYLSGRI
                     VGETIFRLRSDAFSRAVEHDLSFYDEFSSGKVVSRVTTDSEDFANVVGLVVDFFSQLI
                     LVSVFLIYLFHLSPVLSLILLAMAPIAIAIALSFRKIARFVTMNAKRVTAEVNSQIQE
                     SVAGIGVAKGFRREKALWKSFKDNNRLAYRFGLRRGVVLQLIFPAIALASGVGNGIVA
                     YSGGLLARSGTLTMGEWFLFMQAVGFFWWPLLNIASFWSQLQDGLAAAERVFSLIDRE
                     PKVRQTGSYSPARIEGEVEFRGLGFAYSSKESVFEDFSLRIPAGEKLAIVGRTGAGKS
                     SIVKLLMRFYEFQRGELFLDGRDIRGYSLDALRGAIGLVSQDPFLFPGTVADNIRYAR
                     PGASDAEVERAARSLGRGDWVDDLPSGLATATGHRGSSLSMGQRQLVVLARVLL"
     gene            complement(1515..>2978)
                     /locus_tag="VFL04_01800"
     CDS             complement(1515..>2978)
                     /locus_tag="VFL04_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7838463.1"
                     /translation="RAELYSSLLGKSQTFHSRQKVGDIMARATNDVHWLNLMFSPGVM
                     LILDSVLTVAVPMVMVAFIDPRLLLVPSLFVVSLVLTVWNYNRRLNPATAEQRSSFGE
                     LNGTLADALEGIETVKANLGEGRESASFIGGAAKLRDLYIRIARIEGRYWPLLVFAVS
                     WGLGFFHALLLWRQGAISLGQVVGYMLLYNAFRFATFISIWSFNLFQMGLSSAQRVLE
                     TILAHTDLDENPKGISRRIEGRVEFRDVSFSFCGTQGCDGEGGAGSASVLRHVSFVAE
                     PGETVAIVGRTGSGKTSLVRLVNRIFDVDSGQVLVDGSDVREWNLESLRAQIAIIEQD
                     VFLFSRSIRENIAFGAEGASGADIERAAREAQAHEFVSGFEKGYDTEVGERGVTLSGG
                     QKQRIAIARAVLVDPRILILDDSTSAVDSRTEDEIQKAMRRASAGRTTFLITHRLSQI
                     RWADKILLLERGGISDLGTHTELLDRSEDYRRLFARV"
BASE COUNT          519 a          936 c         1081 g          442 t
ORIGIN      
        1 tgaggaggac cctggccagg accacgagct gcctctgccc catggagagg gatgagccgc
       61 ggtggccggt ggcggtcgcg aggcccgagg gcaggtcgtc gacccagtcg cctcggccca
      121 gggaccttgc ggccctctcc acctcggcgt cgctcgcccc gggccttgca tagcggatgt
      181 tgtcggcgac cgtgccgggg aagaggaagg ggtcctggga gaccaggccg atggcgccgc
      241 ggagggcgtc cagggaatag ccgcgtatgt cgcggccgtc gaggaagagc tcgccccgct
      301 ggaactcgta gaagcgcatg aggagcttca cgatcgagga cttgcctgcc cccgtcctgc
      361 ccacgatggc gagcttctcc cccgcgggga tgcggaggga gaagtcctcg aagaccgact
      421 ccttgctcga ataggcgaag ccgaggcccc tgaactccac ctccccctcg atgcgcgccg
      481 gactgtagga gcccgtctgc ctcaccttgg gctcgcggtc gatgagcgag aagacccgct
      541 cggccgccgc gagcccgtcc tggagctggc tccagaacga ggcgatgttg agcagcggcc
      601 accagaagaa gcccacggcc tgcatgaaga ggaaccattc gcccatcgtc agggtgcccg
      661 atcgggcgag gaggccgccg gagtaggcga cgatcccgtt gcccacaccg gaggcgaggg
      721 ctatcgcggg gaagatgagc tggaggacga cgcccctgcg caggccgaag cggtaggcga
      781 ggcggttgtt gtccttgaag ctcttccaca gggccttctc gcggcggaag cccttcgcca
      841 ccccgatgcc ggcgaccgac tcctggatct gggagttgac ctcggcggtg acgcgcttgg
      901 cgttcatggt cacgaagcgg gcgatcttcc tgaaggagag ggcgatggcg atggcgatgg
      961 gggccatcgc gaggaggatc aggctcagga cggggctcag gtggaagagg taaatcagga
     1021 agaccgagac gaggatgagc tgggagaaga agtcgacgac gaggccgacg acgttggcga
     1081 agtcctcgct gtcggtcgtg acccggctca cgaccttgcc cgaggagaac tcgtcgtaga
     1141 aggacaggtc gtgctcgacg gcccgcgaga aggcgtcgga gcgcaggcgg aagatcgtct
     1201 cgccgacgat cctgcccgag aggtagtagc ggaggtagtt gaagagccac tcggccgccc
     1261 cgaagccgat gacggccagg ccgatgaggg cgaagaccca gacctcgggg cgcttggcgg
     1321 ccgcgtcgag ggacttggcc acgaccacct gggccccgaa gtcggccagg gaggcggccg
     1381 cgaggacgca gcccacgagg gctatccgcc tccggaaggg ccggaaatag ctgccgatcc
     1441 tggccaggag ctggcggtcg ctgtattgcc tgtcgtaggc ctcggactcg aggccgtcca
     1501 tcacgaaacc catgtcatac cctcgcgaaa agccgtcggt agtcctcgct gcggtcgagg
     1561 agctccgtgt gggtgcccag gtcggagatc ccgccccgct cgaggaggag gatcttgtct
     1621 gcccacctga tctgggagag gcggtgggtg atgaggaagg tggtgcggcc ggcgctcgcc
     1681 cgccgcatcg ccttctggat ctcgtcctct gtgcgcgagt cgacggccga ggtcgagtca
     1741 tcaaggatga ggatgcgcgg gtcgacgagg acggccctgg cgatggcgat gcgctgcttc
     1801 tgcccgcccg agagggtcac ccccctctcc cccacctcgg tgtcgtagcc cttctcgaag
     1861 cccgagacga actcgtgggc ctgggcctcg cgcgccgccc gctcgatgtc ggccccgctc
     1921 gccccctcgg ccccgaaggc gatgttctcc ctgatgctcc gcgagaagag gaagacgtcc
     1981 tgctcgatga tggcgatctg ggcgcggagg ctctcgaggt tccactcgcg gacgtcggag
     2041 ccatcgacca ggacctggcc cgagtcgaca tcgaagatcc ggttcacgag ccgcacgagg
     2101 ctggtcttgc ccgagcccgt cctgccgacg atcgccacgg tctcgccggg ctcggcgacg
     2161 aaggaaacgt gcctgagcac cgaggccgaa ccggcccccc cctcgccgtc gcagccctgc
     2221 gtgccgcaga aggagaagga tacgtccctg aactccaccc ggccctcgat gcgcctcgag
     2281 atccccttcg ggttctcgtc gaggtcggtg tgggccagga tggtctcgag cacgcgttgg
     2341 gcgctcgaga ggcccatctg gaagaggtta aaggaccaga tcgagatgaa ggtggcgaag
     2401 cggaaggcgt tgtacaggag catgtagccg acgacctggc ccaggctgat cgctccctgg
     2461 cgccagagca ggagggcgtg gaagaagccc aggccccatg agacggcgaa gacgaggagg
     2521 ggccagtagc ggccctcgat cctcgcgatg cggatgtaga ggtcgcggag cttcgccgcc
     2581 ccgccgatga aggaggcgct ctcgcggccc tcgccgaggt tggccttgac cgtctcgatc
     2641 ccctcgaggg cgtcggccag ggtgccgttg agctcgccga agctcgagcg ctgctcggcg
     2701 gtcgcggggt tgaggcggcg attgtagttc cagaccgtga gcacgaggct gaccacgaag
     2761 agcgagggca cgaggaggag gcggggatcg atgaaggcca ccatgaccat gggcaccgcg
     2821 acggtgagca ccgagtccag gatgagcatg acccccggcg agaacattag gttgagccag
     2881 tgcacgtcgt tggtggccct ggccatgatg tcgccgacct tctggcggga gtggaaggtc
     2941 tggctcttgc cgaggaggct cgagtagagc tcggcccg
//
LOCUS       DASSQV010000103         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2141371, whole genome shotgun sequence.
ACCESSION   DASSQV010000103 DASSQV010000000
VERSION     DASSQV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141371"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFL04_01805"
     CDS             complement(<1..617)
                     /locus_tag="VFL04_01805"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7838464.1"
                     /translation="MKTALLLAPKRIAIAETRAPSPGPGEVLIRPEYAGICGSDLSLF
                     LGHRALASFPHVLGHEIVGRVEALGEGTVRLKPGDRVVVEPNYPCGSCGFCLGGRGNI
                     CPSKRSAGVSIPGFFSELCVAPEPFVWPVGDSISGADAATIEPLAVAVAALRRSGAGP
                     GDTAAVIGCGAIGLLLVQTALAQGLRVIAHEPNERKLAMARELGAL"
     gene            complement(622..1440)
                     /locus_tag="VFL04_01810"
     CDS             complement(622..1440)
                     /locus_tag="VFL04_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET7838465.1"
                     /translation="MRFSLAHLTLLSCTPPELTRMAARLGYDFVSLRTIYMGLPNEPN
                     YDLAANRDLLRETKRALADTGLGLHDIELARICDGTDPRRYLPAFEVAAELGCGQAIA
                     SVWTAERNYALDRFREICDLARPLGIRVNLEFMAFSELRTLVEALAFLRAAGSPNAGL
                     LIDSLHFHCSQGSLAELDDVPRGLLDFVHICDGPAAVPATREGLIHRARYARQYLGQG
                     GIDVAGLVNRLPELVYSIELPNPIQVEIHGREGHARLCLETARAYFAAHPRKAG"
     gene            complement(1459..>2413)
                     /locus_tag="VFL04_01815"
     CDS             complement(1459..>2413)
                     /locus_tag="VFL04_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7838466.1"
                     /translation="LEMVRVMRETLRREGVNPDVFQCVEKPSIPLSQYLMSICDLVQA
                     TGGRDMVKAAYSSGVPSLGVGAGNSTMVIDETADIATAAMNTRISKTSDFGSGCSADG
                     NLLVEASIYEAFLAQLQKEGGHLATPDQKARLQAVLWDAEGHRTPDTVAISAQRIAAR
                     AGFEIAADRRFIIVPETNIGKQYPFSSEKLCVVLSIFRYSGFDQALSMVKAIYEVGGK
                     GHSCGIYSHNDDHIHRLALMAPVSRIMVRQPQSKANAGAFDNGMPMTSSLGCGTWGGN
                     ISSENINLKHYMNLTWVSRPIPLDRPSEEELFGEFYGTETL"
BASE COUNT          372 a          755 c          896 g          390 t
ORIGIN      
        1 agggctccga gctcgcgggc catggcgagc ttccgctcgt tgggctcgtg ggcgatgacc
       61 ctgaggccct gggccagggc cgtctggacg aggaggaggc cgatggcccc gcagccgatg
      121 acggccgcag tgtcgccggg cccggccccc gatcgcctga gcgcggccac ggcgaccgcg
      181 aggggctcga tggtggcggc gtcggccccg gagatcgaat cgccgaccgg ccagacgaag
      241 ggctcgggcg cgacgcagag ctcggagaag aaacccggga tgctgacgcc ggcgctccgc
      301 ttgctcgggc agatgttgcc gcggccgccg aggcagaatc cgcagctccc gcaggggtag
      361 ttgggctcga cgacgacccg gtcgccgggc ttcagcctca cggtaccctc gccaagggcc
      421 tcgaccctgc cgactatctc gtgcccgagg acatggggaa acgaggcgag ggccctgtgg
      481 ccgaggaaga gcgagaggtc ggagccgcag atcccggcgt actcgggcct gatgaggacc
      541 tcgccagggc ccgggctcgg ggccctggtc tcagcgatgg cgatccgctt cggggcgagc
      601 aggagggcgg tcttcatggc attagcctgc cttccgggga tgggccgcga agtaggccct
      661 cgccgtctcc agacagagcc gcgcgtgtcc ctcgcggccg tggatctcga cctggatggg
      721 gttcggcagc tcgatcgaat agacgagctc cggcagccgg ttgaccaggc cggctacgtc
      781 gatcccgccc tgacccagat actggcgcgc atagcgggcc cgatggatga ggccctccct
      841 ggtcgcgggg accgccgcgg ggccgtcgca gatgtggacg aagtcgagga gaccccgcgg
      901 cacatcgtcc agttcggcga gggatccctg gctgcagtgg aaatggaggc tgtcgatgag
      961 taggcccgcg ttgggcgagc ccgcggctct gaggaaggca agcgcctcca cgagggtccg
     1021 cagctccgag aaggccatga actcgaggtt gaccctgatc cccagggggc gggccaggtc
     1081 gcagatctcg cggaagcggt caagggcgta gttgcgctcc gcggtccaga cgctcgcgat
     1141 cgcctggccg cagcccagct cggcggcgac ctcgaaggcg ggcagatagc ggcgcgggtc
     1201 ggtgccgtca catatccgcg cgagctcgat gtcgtggagg ccgaggcccg tgtcggccag
     1261 ggcccgcttg gtctcacgca gcagatccct gttcgcggcg agatcgtagt tcggctcgtt
     1321 gggcaggccc atgtagatcg tgcggaggct cacgaagtcg tagccgagcc tggccgccat
     1381 ccgggtcagc tcgggcgggg tacagctgag cagggtcaaa tgcgccaagg agaatcgcat
     1441 cgccgtgcct ccggcaatct aaagcgtttc tgtcccgtag aactccccga aaagctcctc
     1501 ttcgctgggc cggtccaggg ggatgggccg gctgacccag gtgaggttca tgtagtgctt
     1561 gaggttgatg ttctcagagc tgatgttgcc tccccaggtg ccgcagccca ggctcgaggt
     1621 catgggcatg ccgttgtcga aggcgccggc attggccttg gactggggct ggcggaccat
     1681 gatgcggctt accggggcca tgagggcgag gcggtggatg tggtcgtcgt tgtgggagta
     1741 gatgccgcag gaatggccct tgccgccgac ctcgtagatc gccttcacca tgctgagggc
     1801 ctggtcgaag cccgagtatc tgaagatcga gagcacgacg cagagcttct cgctcgagaa
     1861 ggggtactgt ttgccgatgt tggtctccgg cacgatgatg aagcggcggt cggcggcgat
     1921 ctcgaagccg gcccgcgccg cgatcctctg ggccgagatc gcgaccgtgt ccggggttcg
     1981 gtggccctcg gcgtcccaga ggacggcctg gagcctggcc ttctggtcgg gcgtcgcgag
     2041 gtggccgccc tccttctgca gctgggcgag gaaggcctcg tagatcgagg cctcgacgag
     2101 gaggttgccg tcggccgagc agcccgagcc gaaatccgag gtcttgctga tgcgcgtgtt
     2161 catggcggcc gtcgctatgt cggcggtctc gtcgatcacc atggtcgagt tgcccgcgcc
     2221 gacgcccagg gaggggacac cgctcgaata ggcggccttc accatgtccc tgccgccggt
     2281 ggcctgcacg aggtcgcaga tggacatgag gtactgggag agggggatgc tgggcttctc
     2341 cacgcactgg aagacgtcgg ggttgactcc ctcccgcctc agggtctcgc gcatgacgcg
     2401 caccatctcg agg
//
LOCUS       DASSQV010000104         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1519277, whole genome shotgun sequence.
ACCESSION   DASSQV010000104 DASSQV010000000
VERSION     DASSQV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519277"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1907)
                     /locus_tag="VFL04_01820"
     CDS             complement(36..1907)
                     /locus_tag="VFL04_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7838467.1"
                     /translation="MQKRASAFRAIIGAAGFVYVAIVAAALAFFRDPAAAASRPLGVV
                     GLSGILFFAASFLVLGRNAKVYGFDFAQRQHEEEAYGAAITELGGAPLRSLIGFILFM
                     LAWLVGLFAFGQAVGLRDTGRAPLFFFVFSLGMLVAAFVFVITDNLNAKTLLAGTLAA
                     YPRKLREARQQRKIFIIPTFMSFMSFLFAFATAFLVLDRAGGGSENLSIGTFGAVFAL
                     AGAYFLVILALVTVWNVNTGLIYKSVIAQLEGLSAAEKDLTARISIGSVDELGSIAGM
                     VNDFCEGLSKSMGSLKLAQMELNVLGEELGRNANDTAGAVAQISASVDRVKQKTHFQA
                     ASVSESSSAVEQIAKNIESLEIRIAEQAASVAEASASIEEMIGNIGSVTRSIDTMAER
                     FGMLLAAAAEGKATQTESRLRIEQISARSEALLEANKTISTIASQTNLLAMNAAIEAA
                     HAGDAGRGFSVVADEIRRLAETSSGQSKAIRTELTQVQKAIEQVVASSKESESSFARV
                     SEQIGETDALVREVQRAMVEQREGSVQVLEALRAMNDITAEVKVGSREMSAGNATVLA
                     EISRLREATADIGSSMEEMAIGASGIAASTRKVSDMALGARATIRQMDESIGCFKTG"
     gene            complement(2047..2592)
                     /locus_tag="VFL04_01825"
     CDS             complement(2047..2592)
                     /locus_tag="VFL04_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET7838468.1"
                     /translation="MSRAFVDEDASIDAGLDARETKIPVPPGSRNYLTPEGARRLHEE
                     LSGLVESRRPSLASEIARLESSSASSDLDTIAMLRQRLASIDRRIEYLGRMASLAEVI
                     DYRPGQGDRVVFGARVRVEEGSSGERVYRIVGADESDPENGLLSWCSPLARALIGKKA
                     GDCPILHLPGGDRVLRILAVE"
     gene            2648..>3649
                     /gene="sbcB"
                     /locus_tag="VFL04_01830"
     CDS             2648..>3649
                     /gene="sbcB"
                     /locus_tag="VFL04_01830"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HET7838469.1"
                     /translation="MPPSLLWYDLETFGLDPHYDRIVQVAALRTDESFELMGERIVLY
                     CRPSLDYLPSPYSCLVHGITPQFALSEGLGEYELAKRLQAEMSVPGTTVLGFNSLRFD
                     DEFIRNLFYRNFFDPYGREWRNGNTRWDVIDLMRAARDLRPEGIIWPTDDEGRPLFTL
                     GALAKANGIAHDAAHDAMYDVLATIGLAKLVRSRQAKLFDWYFSHRTRESLKSLIDLT
                     DRTPLLHTSVSYTSSRGCTTVVAPIAMDPEDRNRLIALDLRFDPSSIVDLGVEELRRR
                     VFTKGSELEVERLPLVSIRLNRCPFLSPLGALKPEAAARLGIDLDTCRAHLAAIKGET
                     "
BASE COUNT          591 a         1275 c         1167 g          616 t
ORIGIN      
        1 aggcggcctg ttccatttta aggcccgctt ccggcctagc ctgtcttgaa gcagccgatg
       61 gactcgtcca tctgccggat cgtcgccctc gccccaagcg ccatgtccga gaccttccgg
      121 gtgctcgccg cgatcccgct cgcccctatc gccatctcct ccatgcttga accgatgtcg
      181 gccgtcgcct cgcggaggcg gctgatctcg gcgaggacgg tggcgttgcc cgcgctcatc
      241 tcgcgcgaac cgaccttgac ctccgcggta atgtcgttca tggcgcgcag ggcctcgagc
      301 acctggaccg aaccttccct ctgctcgacc atcgccctct ggacctcccg caccagggcg
      361 tcggtctccc cgatctgctc cgacacccgg gcgaatgagg actccgactc cttggatgag
      421 gccacgacct gctctatcgc cttctggacc tgggtgagct cggtccttat ggccttcgac
      481 tggcccgagg aggtctcggc cagacgcctg atctcgtcgg cgaccaccga gaagccgcgg
      541 ccggcgtctc ccgcgtgggc cgcctcgatc gcggcgttca tcgcgagcag gttcgtctgc
      601 gaggctatcg tggagatggt cttgttggcc tcgaggaggg cctcggagcg agccgagatc
      661 tgctcgatcc tgagccgcga ctcggtctgg gtggccttgc cctcggcggc cgcggccagg
      721 agcatcccga agcgctcggc catcgtgtcg atcgacctgg tcacggatcc gatgttcccg
      781 atcatctcct cgatggaggc cgaggcctcg gccacgctgg cggcctgctc ggcgatgcgg
      841 atctccaggg actctatgtt cttggctatc tgctcgacgg cgctcgatga ctccgagacg
      901 ctcgcggcct ggaaatgcgt cttctgcttg accctgtcca cgctcgccga gatctgggcc
      961 accgctccgg ccgtgtcgtt cgcgttcctg ccgagctcct ccccgagcac gttgagctcc
     1021 atctgggcga gcttgaggct gcccatgctc ttcgaaaggc cctcgcagaa gtcgttcacc
     1081 atgccggcga tcgagccgag ctcgtcgacg gagccgatcg agatccgggc cgtgaggtcc
     1141 ttctccgcgg cggagaggcc ctcgagctgg gcgatgacgg acttgtagat gaggcccgtg
     1201 ttcacgttcc agacggtcac gagggccagg atgaccagga agtaggcgcc cgcgagcgcg
     1261 aagaccgcgc cgaaggtgcc gatcgagagg ttctccgacc cgccccccgc cctgtccagc
     1321 acgaggaagg cggtcgcgaa ggcgaagagg aaggacatga agctcatgaa ggtgggtatt
     1381 atgaagatct tccgctgctg tctggcctcc cgcagctttc gcgggtaggc agcgagggtc
     1441 ccagcgagga gggtcttcgc gttgaggttg tccgttatga cgaacacgaa ggccgcgacc
     1501 agcatgccca gggagaagac gaagaagaag agcggcgctc gccccgtatc cctgaggccc
     1561 accgcctggc cgaacgcgaa gaggccgacc agccaggcga gcatgaagag aataaagccg
     1621 atgagcgacc tgaggggagc cccgccgagc tcggttatcg cggctccgta ggcctcctcc
     1681 tcatgctggc gctgggcgaa gtcgaagccg tagaccttcg cgttgcggcc gaggacgagg
     1741 aaactcgcgg cgaagaacag gatgcccgac agacccacga cccccagggg ccgggaggcg
     1801 gccgcggcgg gatcgcggaa gaaggcaagg gcggcggcga cgatggcgac gtacacgaag
     1861 ccggcggctc cgatgatggc gcggaaggcg gacgctcttt tctgcatgga caactccaga
     1921 gtgctggttc cggcatttct ctcacggatg gggcgaagcc acaagggccc ctctccaagc
     1981 caggctccat tatagatcag gaaggggggc gctttcccgc ggtcacggcc cggaaaatcg
     2041 cgtttcctat tcgacggcga ggatccgaag gaccctgtcc cccccgggca ggtggaggat
     2101 cgggcagtcc cctgccttct tgccgatgag ggcccgggcc aagggcgagc accagctcag
     2161 gaggccgttc tcggggtcgc tctcgtcagc gccgacgatc cggtagaccc gctcgcccga
     2221 gctcccctcc tcgacccgga cccgggctcc gaagacgacc ctgtcgccct ggccgggacg
     2281 gtagtcgatc acctcggcga gggaggccat gcggccgagg tactcgatcc tgcggtctat
     2341 cgaggcgagg cgctgcctca gcatggcgat cgtgtcgagg tcggagctgg ccgagctcga
     2401 ttcgagccgg gcgatctccg aggcgaggga gggacggcgg ctctcgacga ggccggagag
     2461 ctcctcatgg aggcggcgcg ccccctcggg ggtcaggtag ttgcgggagc cgggcggcac
     2521 ggggatcttc gtctcccgcg cgtccagccc ggcatcgatc gaggcgtcct cgtccacgaa
     2581 tgcgcggctc atcttccccc attcctcctc gctcgaatat acaccccccg cgtgctatag
     2641 tcctgccatg ccgccctccc tcctctggta cgacctggaa accttcggcc ttgaccccca
     2701 ctacgaccgc atcgtgcagg tcgcggccct gaggacggac gagagcttcg agctcatggg
     2761 cgagcggatc gtcctctact gcaggccgag cctggactat ctgccgagtc cctattcctg
     2821 cctggtccac ggcatcaccc cccagttcgc gctatcggag ggcctaggcg agtacgagct
     2881 cgcgaagcgg cttcaggccg agatgtcggt gcccgggacc acagtcctgg gcttcaactc
     2941 cctcaggttc gacgacgagt tcatccgcaa cctgttctac cgcaacttct tcgaccccta
     3001 tgggcgcgag tggaggaacg gcaacacccg ctgggacgtc atcgacctca tgcgggccgc
     3061 ccgcgacctc aggccggagg gcatcatctg gccgaccgac gacgagggga ggcccctgtt
     3121 caccctcggg gccctcgcga aggccaacgg catcgcccat gacgccgccc atgacgcgat
     3181 gtacgacgtc ctcgccacga tcggcctcgc gaaactcgtg aggagccgcc aggccaagct
     3241 tttcgattgg tacttctcgc accgcacccg ggagtccctc aagagcctca tcgacctcac
     3301 ggaccggact cccctcctcc acacctcggt ctcgtacacc tcgagccggg gctgcacgac
     3361 cgtagtcgcc ccgatcgcga tggacccgga ggaccgcaac cggctcatcg ccctcgacct
     3421 ccgcttcgat ccctcctcga tcgtcgacct cggcgtcgag gagctccgcc gccgggtctt
     3481 cacgaagggt tcggagctcg aggtcgaaag gctgcccctc gtaagcatcc gcctcaaccg
     3541 ctgtcccttc ctctcgcccc ttggcgccct caagcccgag gcggccgcga ggctggggat
     3601 cgacctcgac acctgccgcg cccatctcgc cgccatcaag ggcgagacc
//
LOCUS       DASSQV010000105         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2017863, whole genome shotgun sequence.
ACCESSION   DASSQV010000105 DASSQV010000000
VERSION     DASSQV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017863"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..848
                     /locus_tag="VFL04_01835"
     CDS             312..848
                     /locus_tag="VFL04_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7838470.1"
                     /translation="MLAFLMSRLAEGQTHPETGKETPLPRIAADLEPIRVLLDARSEE
                     AWTLAALARKAGYSRYHFLRSFRAVFHETPLRYLSGRRLAKAKRLLADTDLTVTEICC
                     EVGFESLGSFSTLFLARVGWSPSVYRARVLAQRSRPLDHIPACHIGRYGLGSISAPAS
                     NPREAGISEKRDSRSGGP"
     gene            869..1285
                     /locus_tag="VFL04_01840"
     CDS             869..1285
                     /locus_tag="VFL04_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7838471.1"
                     /translation="MITRLGHANILVLDQQKAYEIYVNRLGFKVKTDMVMEGGFRWLT
                     LTAPGDPELELILAEPKPPMFEAEDAVLVRRLLERNALGAGVWECDDCRATYKALKAK
                     GIEFLKEPTEEFYGIEAVFKDGCGNWFSLTERKRQA"
     gene            complement(1314..2177)
                     /locus_tag="VFL04_01845"
     CDS             complement(1314..2177)
                     /locus_tag="VFL04_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7838472.1"
                     /translation="MQTQKRMLPVAAVLVAAGLGASSGLYIKGIGLSSLALAGFRMGV
                     PFFAVLPYLARRGRMLGEKSERKGLWLASGINAVRMLLFVMAYKLTTIGNAVVLLYLW
                     PIFALVFDCVRFRRWPERAQLGLLALAFGGVVTMNYHRGFSLSSEDFLGSALMILSAC
                     LFAATVIMFKKALAKVHETDALFFQNAIGAIVYAPFLFAELGGASLHDIGLGLSYGLA
                     VGLVGFGLFFFGMKRLPLFQYSALAYSEVPFGLALGVLVLGEDISGTQLAGAAMILGA
                     SFVAQRLRTRA"
     gene            complement(2221..>2789)
                     /locus_tag="VFL04_01850"
     CDS             complement(2221..>2789)
                     /locus_tag="VFL04_01850"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET7838473.1"
                     /translation="SLEALKAWDVVILSDIGANTLLLHPNTWLRGESMPNRLELLRQY
                     AEAGGGLAMCGGYLSFAGIYASAKYYRTPIEEVLPVSIHTFDDRVESPQGARATVLVK
                     DHPVLEGIPGEWPALLGFNELVAKSDATILARIGEHPLLTLRSVGEGRTLAWASDIGP
                     HWCPTSFAEWEGYARLMSQAVEWLAGRR"
BASE COUNT          513 a          876 c          953 g          447 t
ORIGIN      
        1 atgtgcgccc tttgcatgcg tcacctcctt attcctcgga aggccgctgg ccaggtggcc
       61 gggcaggact aaggtactgc cggggccgcc ggatcgacaa aatccgggcg gggtccggtc
      121 gcgggggacc aggcggaagg cacggtaagg tgggcgcgac agcactcccc gcggcgccta
      181 gacggagcca caacccagtc tcaacacgag gcctgagccc atgcccttct tgtcgagccc
      241 tggcgatcgt cgtaggatcg gtgcatggca agccgaatgt cccgatctct ctccaaagga
      301 gccttaaggc catgctggcc ttcctgatga gccgcctggc tgaggggcaa acgcatccgg
      361 agactgggaa agaaacccca ttgccgagga tcgccgccga cctcgagccc atccgagtcc
      421 tgctcgatgc ccgctccgag gaggcctgga ccctcgcggc cctcgcgagg aaggcgggct
      481 actctcgtta tcatttcctc cgcagcttcc gcgccgtgtt ccacgagacc cctctccgct
      541 atctgagcgg acggcgtctc gccaaggcga aacggcttct ggccgacacc gacctgacgg
      601 tcaccgagat ctgctgcgag gtcggtttcg agagcttggg atccttcagc acgctgttcc
      661 tcgcgagggt cggctggtcg ccgtctgtct atcgggcccg ggtcctcgcg cagcgctccc
      721 ggccactgga ccacattccc gcctgccaca tcgggcgtta cggcctcggg tcgatttccg
      781 ccccggccag caatccccga gaagccggga tctccgaaaa gcgggatagt cgttctgggg
      841 ggccgtgaag gcttcccgag gaggcatgat gatcacgaga ttgggccatg cgaacatcct
      901 ggtgctcgac cagcaaaagg cctacgagat ctatgtgaac aggctcggct tcaaggtcaa
      961 gaccgacatg gtgatggagg ggggcttccg ctggctcacc ctcacggccc cgggggatcc
     1021 cgagctcgag ctcatcctcg ccgagccgaa gccgccgatg ttcgaggccg aggatgcggt
     1081 gttggtcagg cgtctcctcg agcgcaacgc cctcggcgcc ggggtctggg agtgcgatga
     1141 ttgccgcgcg acctacaagg cgctcaaggc gaagggaatc gaattcctca aggaacccac
     1201 agaggagttc tacggcatcg aggcggtctt caaggacggc tgcggcaatt ggttctcgct
     1261 gactgaaagg aagcggcagg cctgatgcgg gtgggcctgg gggtcctgtt gctttaggcc
     1321 cgcgttcgca gccgctgggc gacgaagctc gcgccgagga tcatggccgc acccgccagc
     1381 tgcgtgcccg agatgtcctc cccaaggacc aggacgccca gggctaagcc aaagggcacc
     1441 tcgctgtagg cgagggcgct gtactggaac aggggcaggc gcttcatccc aaagaagaag
     1501 aggccgaagc cgacaaggcc gacggcgagg ccataggaga gccccaggcc gatgtcgtgg
     1561 agcgatgctc ctccgagctc ggcgaagaga aaaggcgcat agacgatggc cccgatcgcg
     1621 ttctggaaaa agagggcgtc cgtctcatgg accttggcca gggctttctt gaacatgatg
     1681 accgtcgcgg cgaagaggca ggcggagagg atcatgaggg cgctgccgag gaagtcctcc
     1741 gaggagaggg agaagccgcg gtgatagttc atcgtgacga cgccgccgaa ggcgagggcg
     1801 agcaggccaa gctgggcccg ttccggccaa cgcctgaacc tgacgcagtc gaagacgagg
     1861 gcgaagatcg gccagaggta gaggagcacc accgcgttgc cgattgtcgt cagcttgtag
     1921 gccatgacga agagcagcat ccgcacggcg ttgatgccgc tggccagcca caggcccttc
     1981 cgctcggatt tctcgccgag catgcggccc ctgcgggcaa ggtagggcag gaccgcaaaa
     2041 aaggggacac ccatccggaa acccgcgagg gcgaggctcg acagcccgat gcccttgatg
     2101 tacaggccgc tcgaggcccc aagcccggcg gcgaccagga cggccgccac gggcagcatt
     2161 ctcttttgtg tttgcatggg gccaactatt gggccggaat ggctcccgcg gtcaagcgcc
     2221 ctagcgcctc cccgccagcc actcgaccgc ctgggacatc aggcgcgcat agccttccca
     2281 ctccgcgaag gatgtggggc accaatgggg accgatgtcg gaggcccatg ccagggtcct
     2341 cccttcgccg acggaacgca gggtgaggag ggggtgctcg ccgatcctcg ccaggatcgt
     2401 ggcgtcgctt tttgcgacca gctcgttgaa gccgaggagg gccggccatt cgcccgggat
     2461 gccttccagc acggggtgat ccttcacgag cacggtggcc cgggcgccct gcgggctctc
     2521 cacgcgatca tcgaaggtat ggatgctgac gggcaggacc tcctcgatgg gggttctgta
     2581 gtacttggcc gaggcgtaga tgcctgcgaa ggagaggtag ccgccgcaca tcgcgagccc
     2641 ccctccggcc tcggcgtatt gcctgaggag ctcgaggcgg ttgggcatgc tctcgccgcg
     2701 gagccaggta ttggggtgga ggaggagggt gttggcgccg atgtcgctca ggatgaccac
     2761 gtcccaggcc ttcagggcct caagcgaga
//
LOCUS       DASSQV010000106         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_525214, whole genome shotgun sequence.
ACCESSION   DASSQV010000106 DASSQV010000000
VERSION     DASSQV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525214"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFL04_01855"
     CDS             <1..409
                     /locus_tag="VFL04_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7838474.1"
                     /translation="GIVIDVDGRVLLLVRAREPARGRLALPGGFVEPGERAEDAALRE
                     CREEIGWAPERIEFLASYPNLYPYKGIPYATCDVYFRAWAPGLDPELLDFDGAEASAI
                     RLESLSGITPDQVAFESTCRALKRHMRQPRTGA"
     gene            complement(446..2116)
                     /locus_tag="VFL04_01860"
     CDS             complement(446..2116)
                     /locus_tag="VFL04_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7838475.1"
                     /translation="MGIATLGAIAIGQYEEAVGVMVFYSIGEVLQHSAASRSRASIRS
                     LLDLRPDFARLRREGSWVELPPELAVPGDEFLVKPGERLPLDGRVIEGEAFLDTSALT
                     GESMPRRAGPGSPVLAGSVSLDGALVIAASRVAGESSAARIALLVENAAHLKARTERW
                     VTSFASVYTPIVVAMAAAIAFLPPLLFAGQELRTWCYRALVMLVISCPCALVVSVPLG
                     YFGGIGGAARRGILVKGSQVLDALARCRTVAFDKTGTLTRAALTVRSIEPAPGRSADE
                     VLAWAAAAESLSRHPIAASLRAEAGARSLSIGTEDEASEVRELPGAGIAARIGGRRVL
                     AGNERLLDLEGVGHPPIEAEDTRVLVALDGQYAGSIALGDEAKGDARRAVDELRRLGV
                     SRLALLTGDSGPVARALAEDLGIDETYAELLPEGKLEVLARIIAETGAAGGGARGGTA
                     FVGDGINDSPVLALADVGIAMGAGSDAAVETADVVLLTDEPSRVPEAISRARRTRRIV
                     AQNIVFALGVKTAFLAMGAVGVAVMWEAVIADVGVALLAVANSLRAMK"
BASE COUNT          363 a          788 c          836 g          310 t
ORIGIN      
        1 ggggatcgtg atcgatgtcg acggcagggt cctcctcctg gtgagggcca gggagcccgc
       61 ccgcggccgc ctggccctgc ctggcggatt cgtggagccc ggggagcgcg ccgaggacgc
      121 ggccctacgg gaatgccggg aagagatagg ctgggccccc gagcggatcg agtttctcgc
      181 ctcctacccg aacctatacc cctacaaggg gattccctac gccacctgcg atgtctactt
      241 ccgcgcttgg gctcccggcc tcgaccccga gctcctcgat ttcgacggag ccgaggcgag
      301 cgcgatcagg cttgagagcc tcagcggaat aacgcctgac caggtcgcct tcgagtccac
      361 ctgccgcgcc ctgaagcggc acatgcggca gcccaggact ggggcctagg gcctcggccc
      421 gggagcgcgc cggggcgatc ccgtctcatt tcatcgccct aagggagttc gcgacggcga
      481 ggagggcgac accgacgtcg gcgatgacgg cctcccacat cactgccacg cccactgccc
      541 ccatggcgag gaaggccgtc ttgacgccga gggcgaagac gatgttctgc gcgacgatgc
      601 ggcgggtgcg gcgggccctg gagatggcct cgggcacccg cgaaggctcg tcggtcagga
      661 ggacgacatc ggcggtctcg acggcggcgt cggatccggc gcccatcgcg atgcccacat
      721 cggcgagggc gagcacaggg gagtcgttta tgccgtcgcc gacgaaggca gtcccgcccc
      781 tggccccgcc tcccgccgcc ccagtctccg cgatgatgcg cgcgaggacc tcgagcttgc
      841 cctcggggag gagctcggca taggtctcgt cgatcccgag gtcctcggcg agagcccggg
      901 cgacgggacc ggaatccccg gtcaggaggg cgaggcgcga gactccgagc cggcggagct
      961 cgtccaccgc ccgcctggcg tcgcccttgg cctcgtcgcc cagggctatc gatccggcat
     1021 attggccgtc gagggcgaca aggaccctcg tgtcctcggc ctcgatcggg ggatgcccga
     1081 ccccctcaag gtcgaggagc ctctcgttgc ctgccaggac ccttctgccg ccgatcctcg
     1141 cggcgattcc ggccccgggc agctccctga cctccgaggc ctcgtcctcg gtcccgatgg
     1201 acagcgacct cgccccggcc tcggcccgga gtgaggcggc gatggggtgg cgggagaggg
     1261 attcggccgc cgcggcccag gcgaggacct cgtcggcgct ccggccaggg gccggctcta
     1321 tcgacctgac ggtcagggca gccctcgtga gggtccccgt cttgtcgaag gcgacggtcc
     1381 tgcagcgggc gagggcatcg aggacctggg agcccttgac caggatcccc ctcctcgccg
     1441 ccccgccgat tccgccgaaa tagcccaagg gcacgctcac gaccaaggcg caggggcagg
     1501 agatcacgag catgacgagg gcccggtagc accaggtcct gagctcctgg ccggcgaaga
     1561 gaagcggggg gaggaaggcg atggccgcgg ccatcgcgac cacgatcggg gtatagaccg
     1621 aggcgaagct tgtgacccag cgctccgtgc gggccttcag gtgggcggcg ttctccacga
     1681 ggagggctat cctggccgcc gaggactcgc cggcgacgcg gcttgccgcg atgacgaggg
     1741 cgccgtcgag ggagaccgag ccggcgagca ccggggaacc cggtccggcc cgccttggca
     1801 tcgactcgcc cgtgagggcc gaggtatcga ggaaggcctc tccctcgatc accctgccgt
     1861 cgagggggag gcgctcgccg ggcttcacga ggaactcgtc gccggggacg gccagctcgg
     1921 gcgggagctc gacccaggag ccctcccgcc taagcctcgc gaagtccggc ctgaggtcga
     1981 gcaggctccg tatcgaggcc ctgctccgcg aagcggccga atgctggagg acctcgccga
     2041 tcgagtagaa gaccatgacc ccgacggctt cctcgtactg gccgatggcg atggctccca
     2101 aggtagcgat gcccatgagg aagagctcgt cgaagacctt gccgcggacg atgtttcggg
     2161 cggcgccgag gaggaccttc cagcccgaga cgacctaggc gaaggcgagg aggccgaggc
     2221 cgatggagga ggccgggctg gagtggacaa agttgacgag gacgaggccg aggattccag
     2281 cggccgtcgc gaggagg
//
LOCUS       DASSQV010000107         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1022396, whole genome shotgun sequence.
ACCESSION   DASSQV010000107 DASSQV010000000
VERSION     DASSQV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022396"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFL04_01865"
     CDS             complement(<1..372)
                     /locus_tag="VFL04_01865"
                     /inference="COORDINATES: protein motif:HMM:NF016365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HET7838476.1"
                     /translation="MFATFVNALAIIAGSLLGLLIKKGLPKRYEQVIYTASGVTTLVI
                     GMQMAFKTSHILAFALALILGGLLGTLLDIEGGVLKLGEALRRRFAKNDEGGHFAYGF
                     LSASVLFCSGAMAIVGSFKAGA"
     gene            474..749
                     /locus_tag="VFL04_01870"
     CDS             474..749
                     /locus_tag="VFL04_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838477.1"
                     /translation="MEGTSCVFCANCIHCKLVPSPADMEGRYRLRVRCEAGKWRKKLG
                     EEKVYKYFTVARRSIPACEAYEDMGESAEFMRDLRSLLPTTDEIYST"
     gene            761..1162
                     /locus_tag="VFL04_01875"
     CDS             761..1162
                     /locus_tag="VFL04_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838478.1"
                     /translation="MKASISAISTAVAVLALLIACSSAPKQIPEGLSSQELVQRAQEA
                     SDVYKYEVAKAYYQALLERFASDPGAVAQGEYEIAFIAYKQERFQEAKDGLNRLLARY
                     SGPEGKSFPPAYQVLAQKVLDRIAELQKGNP"
     gene            complement(1242..1523)
                     /locus_tag="VFL04_01880"
     CDS             complement(1242..1523)
                     /locus_tag="VFL04_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838479.1"
                     /translation="METRNGLITFGIFVLLFAFTFVFCLEALIVNSTFYGVLALIGFV
                     VSIATALFYGLMAKQNGEALAPWFNTYALLVGIVFVWYLTRVGTAFRWW"
     gene            complement(1598..>3093)
                     /locus_tag="VFL04_01885"
     CDS             complement(1598..>3093)
                     /locus_tag="VFL04_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET7838480.1"
                     /translation="MGLGFREADLDRRARELSGGWQMRIALAKVLLENPDIMLLDEPT
                     NYLDLEARSWLEDFLAAYKGGVLLVSHDRYFLDVTVREVYELWNGKLSRYPGTYSQYE
                     ERRAKELEGIFEAWERQQEEIQRLEDFIRRFRYQATKASLVQSRVKQLEKVVPIEIPE
                     GMKKIHFSFPPAPRSGKVAVRLEGIRRAYGANRVIENLDLEIERGMKVAFVGPNGAGK
                     STLMRIVAGADGDFEGKLGFGSGIEVAYFAQDTADLMDGEATVEEEAESLCPTELLPK
                     IRNLLGAFLFRGDDIHKSVRVLSGGERSRLALLKMLLRPANLLVLDEPTNHLDLTSKD
                     VLLEALKAFEGTVLFVSHDRLFIEELATRVLELEAGSSPRWYVGDYGYYLEKKASSQG
                     EAPAPAAAKPGSSPAPAPKALSYEEDKARKAKQRKLQRREEEIIARLEALGAQKAAAE
                     REMGLPGNYSDGERMRRLAASVEELDREAESLNAEWEAVAEELATGA"
BASE COUNT          537 a         1005 c         1042 g          509 t
ORIGIN      
        1 ggcgcccgcc ttgaacgagc cgacgatggc catggcgccc gagcagaaga gcaccgaggc
       61 gctcaggaag ccgtaggcga agtggccgcc ctcgtcgttc ttcgcgaagc gccgcctcag
      121 ggcctcgccg agcttcagga ccccgccctc gatgtcgagg agggtgccaa ggaggcctcc
      181 caggatgagg gcaagggcga aggcgaggat gtggctcgtc ttgaaggcca tctgcatgcc
      241 gatgaccagg gtggtgacgc ccgaggcggt gtagatcacc tgctcgtagc gcttgggaag
      301 ccccttcttg atcaggaggc cgaggagtga gcccgctatg atggcgagcg cgttgacgaa
      361 ggtggcgaac atcgatgtct ccgcaaaaga ggggtcccgg gcttttcaag gccgcgcctt
      421 gaccgcgccg gagggagtat agcataattg tccgcgaagg gaggagtccg ggcatggaag
      481 gcacgtcctg cgtcttttgc gctaactgca tccactgcaa gctcgttccc tcccccgccg
      541 acatggaggg ccgctaccgc ctccgcgtcc gctgcgaggc gggcaagtgg cggaaaaagc
      601 tcggcgagga gaaggtctac aagtacttca ccgtcgcccg caggagcatc ccggcctgcg
      661 aagcctacga ggacatgggg gagagcgcgg agttcatgcg cgacctgcgc agcctcctgc
      721 ccacgaccga cgagatctac tcaacctgac ggagcttccc atgaaagcca gcatctccgc
      781 gatttcgacg gcagtggccg tgctcgccct cctcatcgcc tgctcgagcg cccccaaaca
      841 gatccccgag ggcctgagct cccaggagct cgtccagagg gcccaggagg cgagcgacgt
      901 ctacaagtac gaggtggcga aggcctacta ccaggccctc ctcgagcgct tcgcctcgga
      961 tcccggggcc gtggcccagg gcgagtacga gatcgccttc atcgcctaca aacaggagcg
     1021 cttccaggag gccaaggacg gccttaacag gctcctcgcc cgctattccg gccccgaggg
     1081 caagagcttc ccgcccgcct accaggtcct cgcccagaag gtcctggaca ggatcgccga
     1141 gctccagaag ggcaatccct agccgcccct gccaaaaaat gggccgcgga gcctgagggc
     1201 ttccgcggcc ttggggtcct agccccgcgc tggagaagga tttaccacca gcggaaggcg
     1261 gttcccaccc tggtcaggta ccacacgaag acgatgccca ccagaagggc ataggtgttg
     1321 aaccaggggg cgagggcctc gccgttctgc ttcgccatga ggccatagaa gagggcggtc
     1381 gcgatgctca ccacgaaacc gataagggcc aggactccgt agaaggtgct gttcacgatg
     1441 agggcttcga ggcagaagac gaaggtgaag gcgaaaagca gcacgaagat gccgaaggtt
     1501 atgaggccgt tccgggtttc cataggttcc gctcctttgc tcgcatgtcg atgattttca
     1561 aaaccagtat agaccgcccc aatggaattg tccacattca tgcgccggta gccagctcct
     1621 cggccacggc ctcccactcc gcgttgagcg actcggcctc gcggtcgagc tcctcgacgc
     1681 tcgccgcgag acggcgcatc cgctccccgt ccgaatagtt gccgggcagg cccatctccc
     1741 gctcggccgc cgccttttgg gccccgaggg cctcgagccg ggcgatgatc tcctcctccc
     1801 gcctctgcag cttcctctgc tttgccttgc gggccttgtc ctcttcgtag gagagggcct
     1861 tgggggcggg ggccgggctt gagccgggct tcgcggcggc cggggccggg gcctcaccct
     1921 ggctcgaggc cttcttctcg aggtaatagc cgtagtcgcc gacgtaccag cgggggctcg
     1981 agccggcctc gagctcgagg acccgggtcg cgagctcctc gatgaagagc ctgtcgtggg
     2041 aaacgaaaag gacggtgccc tcgaaggcct tcagggcctc gaggaggacg tccttcgagg
     2101 tcaggtcgag gtggttcgtc ggctcgtcga ggacgaggag gttggcgggc ctgaggagca
     2161 tcttgaggag ggccagacgg gagcgctcgc cgccggagag gacgcgcacg ctcttgtgga
     2221 tgtcgtcgcc gcggaagagg aaggcgccga ggaggttgcg gatcttgggc aggagctcgg
     2281 tggggcagag ggactcggcc tcctcctcca ccgtggcctc cccgtccatg aggtcggccg
     2341 tgtcctgggc gaaataggcc acctcgatcc cggagccgaa gccgagcttg ccctcgaagt
     2401 cgccgtctgc cccggcgacg atccgcatga gggtggactt gccggccccg ttggggccca
     2461 cgaaggccac cttcatgccc cgctcgatct cgaggtcgag gttctcgatg acccggttcg
     2521 ccccgtaggc ccgcctgatc ccctcgagcc tgaccgccac cttgcccgag cgcggggccg
     2581 gcgggaagga gaagtggatc ttcttcatcc cctcggggat ctcgatcggc accaccttct
     2641 cgagctgctt cacccggctc tggaccaggg aggccttggt ggcctggtag cggaagcggc
     2701 ggatgaagtc ctcgagccgc tggatctcct cctgctggcg ctcccaggcc tcgaagatcc
     2761 cctcgagctc cttggcccgg cgctcctcgt actgggaata ggtgccggga tagcgcgaga
     2821 gcttgccgtt ccagagctcg tagacctccc gcacggtgac gtcgaggaaa tagcggtcgt
     2881 gggacacgag gaggaccccg cccttgtagg cggccaggaa atcctcgagc caggagcggg
     2941 cctcgaggtc gaggtagttc gtcggctcgt cgaggagcat gatgtccggg ttctcgagga
     3001 ggaccttggc gagggcgatg cgcatctgcc agccgcccga gagctccctg gccctccggt
     3061 cgaggtcggc ctccctgaag ccgaggccca tga
//
LOCUS       DASSQV010000108         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2266812, whole genome shotgun sequence.
ACCESSION   DASSQV010000108 DASSQV010000000
VERSION     DASSQV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266812"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFL04_01890"
     CDS             <1..600
                     /locus_tag="VFL04_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838481.1"
                     /translation="LVAKDREKGLAYAIAPIWHLRPYMNRASPLPMAAFNWNDAELAE
                     WLAWEWAAKRQGGRDPKPAAVSYAAYWLAAAVVTRMQGPGSLQLARWREGVRDRRTFQ
                     LLASDLQGQVRGILASHTDPKDREATLRRIERNWLFSYQQQYSRRFLTNAYQGFGKDG
                     WVDPLALEQIEGDTLGWGALEKLLPEGQGDAEALLRILK"
     gene            618..>2520
                     /locus_tag="VFL04_01895"
     CDS             618..>2520
                     /locus_tag="VFL04_01895"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7838482.1"
                     /translation="MATAKRRTGSSARGSWIGRGSRGVAPAAVLILVALGQAVWAMGT
                     KEPSTQSADGKENWQHSFDISAMPAGKFNVYVEAKDLAGNKAVTGPFNIVIDPKSDLP
                     VARILNPLPAMRVGGDLTIVGTCDDDDGVAKVEVSVDGGDWVEAEGTALWSLYLKTAS
                     IPDGRRSLSVRGTDINGLVGKETKLFFNLDRFKPVVQVATPEPGALVAGAIRLQGEAR
                     DANGLRSLEMSLDGGTKYAKLELKRGRAIEKAGFSAAIDTRKIGDGPKVIWFRCVDGV
                     GSSSTTAYLLIIDNTKPTIRLVRPQPGQAVNGLFSVVGSVNDAVGIKRLSYEFAGSQK
                     GEIPLRPGDPYFALDLDAGPLKSANAPVVLIAEDRIGNVTRLAIAPKIDREADKPVVL
                     PLFPQAEGRVRAGEQIWGSVSDDDGVAAVRWSIDGGPPTQTPASEAFALGLPDLGSGR
                     HIITMAGVDTNGLVGNPRTLPFVYDRGRGQVSFLRLSAQAQGKAGPASRDFSQGIEVK
                     VDGGEQLEGMVSMPNPPTKAEWSIGTAAARKLELQRTDKTAKDSKGGDWGFRIPMDRS
                     LPYGFAPISVRVSDGFGNEAMAQALVYVTNYASDREPSGFRFEEGRASEDGSVRLGPT
                     EPFQGVFFRE"
BASE COUNT          448 a          863 c          859 g          350 t
ORIGIN      
        1 ctcgtggcga aggacaggga gaagggcctc gcctatgcga tcgcccctat ctggcacctg
       61 cggccatata tgaatcgcgc atcgcccctg cctatggcgg ccttcaactg gaacgacgcg
      121 gagctcgccg agtggctggc ctgggagtgg gcggccaagc ggcagggcgg cagggacccg
      181 aagccggcgg cggtctccta cgccgcatac tggctcgccg cggcagtcgt caccaggatg
      241 cagggccccg gctccctcca gctcgcgagg tggcgcgagg gcgtcaggga ccgtcggacc
      301 ttccagctcc tcgcgagcga ccttcagggc caggtgcgcg gcatcctcgc ctcgcacacc
      361 gacccaaagg accgcgaggc gaccctcagg cggatcgagc gcaactggct cttctcctac
      421 caacagcagt attcgcgccg cttcctcacc aacgcctacc agggcttcgg caaggacggc
      481 tgggtcgatc ccctcgccct cgagcagatc gagggtgaca cgctcggatg gggcgcgctg
      541 gagaagcttc tgcccgaggg gcagggcgac gcagaggccc tcctccggat cctgaaatag
      601 gaaaaaagga ccgcacgatg gcgacagcga agcgcaggac tggcagctcg gctcggggct
      661 catggatcgg tcggggaagt cgaggcgtcg cccccgcggc ggtgctcata ctcgtggccc
      721 ttggccaggc ggtctgggcc atggggacga aggagccctc gacccagagc gccgacggca
      781 aggagaactg gcagcacagc ttcgacatct cagccatgcc cgcgggcaag ttcaacgtct
      841 acgtcgaggc gaaggacctg gccggcaaca aggcggtgac ggggcccttc aacatagtca
      901 tcgaccccaa atccgacctg cccgtcgcgc gcatcctcaa ccccctaccc gcgatgcggg
      961 tcggcgggga cctcaccatc gtggggacct gcgacgacga cgacggggtg gccaaggtcg
     1021 aggtcagcgt cgacggcggc gattgggtcg aggccgaggg cacggcgctc tggagcctct
     1081 acctcaagac agcctcgatc cccgacggca ggagaagcct gtccgtgagg ggcaccgaca
     1141 tcaacggcct cgtcggcaag gagaccaagc tcttcttcaa cctcgaccgc ttcaagcccg
     1201 tggtccaggt ggccacgccc gagccgggcg ccctcgttgc gggagccatc aggctccagg
     1261 gcgaggcccg cgacgccaac ggccttcggt ccctcgagat gtccctggac ggcgggacca
     1321 agtacgccaa gctcgagctc aagaggggga gggcgatcga gaaggcgggc ttcagcgccg
     1381 ccatcgacac gaggaagatc ggcgacggcc cgaaggtgat ctggttccgc tgtgtcgacg
     1441 gcgtgggctc ctcgtccaca acggcctacc tcctcattat cgacaacacg aagccgacga
     1501 tcaggctcgt gaggccccag cctggccagg ccgtcaacgg cctcttctcg gtcgtcggct
     1561 cggtgaacga cgccgtcggc atcaagcgcc tgtcctacga gttcgcgggc agccagaagg
     1621 gcgagatccc cctaaggccg ggcgacccct atttcgccct ggacctcgac gccggcccgc
     1681 ttaagtcggc caacgcgccc gtcgtgctga tcgccgagga caggataggc aacgtcacga
     1741 ggctcgccat cgcgccgaag atcgaccgcg aggccgacaa gcccgtggtc ctccccctct
     1801 tcccccaggc cgagggccgg gtcagggccg gcgagcagat ctggggctcg gtgagcgacg
     1861 acgacggtgt ggccgccgtc cgctggtcca tcgacggggg cccgcccacc cagacgcccg
     1921 cgtccgaggc cttcgcgctc ggcctgcccg atctcggctc ggggcgtcac atcatcacca
     1981 tggcgggcgt cgacacgaat ggcctggtag gcaaccccag gaccctgccc ttcgtctatg
     2041 acaggggccg gggccaggtc tccttcctca ggctctcagc ccaggcccag ggcaaggccg
     2101 ggccggcgag cagggatttc tcccagggca tcgaggtcaa ggtcgacggg ggcgagcagc
     2161 tcgagggcat ggtgtccatg ccgaatcccc cgaccaaggc cgagtggagc atcggtacgg
     2221 cagcggccag gaaactcgag ctccagagga cagacaagac ggcaaaggac tccaagggcg
     2281 gggactgggg cttccggatc ccgatggaca ggagcctgcc ctatggcttc gccccgatat
     2341 cggtgcgggt gagcgacggt ttcgggaatg aggcgatggc ccaggccctc gtctatgtca
     2401 ccaactacgc gagcgaccgc gagcccagcg gcttccgctt cgaggagggc agggcctccg
     2461 aggacggcag cgtgcggctc ggcccgaccg agcccttcca gggcgtcttc ttcagggaga
//
LOCUS       DASSQV010000109         4780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_276863, whole genome shotgun sequence.
ACCESSION   DASSQV010000109 DASSQV010000000
VERSION     DASSQV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276863"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFL04_01900"
     CDS             complement(<1..621)
                     /locus_tag="VFL04_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET7838483.1"
                     /translation="MQIIGESWRDEEGRRLVLRGVNLGGDCKVPSRPEGATHLKEGFY
                     QRRGVSFVGRPFPLAEADAHFRRLKRWGMNFLRLLVTWEAVEHDGPGIYDHEYLDYIE
                     AIVAKACEWGFAVLIDPHQDVWSRWTGGDGAPIWTLEAVGLEPERLHASGAALLHQEL
                     GEAYPRMKWFSNGSRLGCATMFSLFFGGDAFAPGIEPLAGGSGESIQ"
     gene            721..1494
                     /locus_tag="VFL04_01905"
     CDS             721..1494
                     /locus_tag="VFL04_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7838484.1"
                     /translation="MDKGDVEGRVLFDDGEHKFIWLGTDQADRKGIVQTLQYLVVDKG
                     RGWLLDPGGVHLFSQVVASASRYISLDRIEGIFFSHQDPDVSSGIALWLGVTSAKVYI
                     SSLWTRFIPHFGLVDQTRITAIEDMGKALVLGSGARLSFVPTHFMHSPGAWSLFDERA
                     RILFSADVGAAVFPEGQETLFIDDFATVLPFTEGFHKRYMASNAVVRRWADIVRRLEP
                     QMIAPQHGGIYRGRAVGDFLSWLSSLRCGVDLLDELYRI"
     gene            1502..2356
                     /locus_tag="VFL04_01910"
     CDS             1502..2356
                     /locus_tag="VFL04_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7838485.1"
                     /translation="MQEDILSNFRVALDRFAIGYNKSVVLSSVTQRSLGILDTSMRGI
                     EESLSTIVAAFEEIRATGSSTSENAVRIDEMMSAILVKNASMDSDVVERMDDIERAAS
                     DARHIDGLFRELQGKTKSIENVTGAIRDVSDRTNILAINASIEAARAGSVGKGFRIIA
                     NEVRNLAGQTGDFAKEIESTIGEFRSSVAVIDERMRGFIELLERFRSSFAEILANFKA
                     NAVSIDEAGNFLTQIAAAIQEETKALSEGLGSLEQISANVKDTHAVFSALSSSHAFLD
                     TLLDKGEA"
     gene            2359..3375
                     /locus_tag="VFL04_01915"
     CDS             2359..3375
                     /locus_tag="VFL04_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HET7838486.1"
                     /translation="MEVGGAGPRRPLAVITDDRFGESAIEQAILGAAGVELVVAKCRS
                     SAEVAAAGREADALLVNLAPADAAAIEALERCRVIARYGIGLDNVDQEAARRRGIRVA
                     NVPGYCDTEVAEHALALILALSRGIARRDRVVRGGGWGGTPFGRRIAGTTLGILGFGG
                     TGQALARIALALSFREILVWSPHISAGRIAEALGPAPAALGAVVRPAGFEELLSRSDW
                     VSVHLPLKGETRGIVGASAMAMMKKEACLVNVSRGAVLDEEALIDAMATGALAGVGLD
                     VFVAEPLPQGNRLRSFPNAVFTDHNAYASTESIAELRRRTAENALAGLAAAGLVPSGP
                     GLPR"
     gene            complement(3350..4123)
                     /locus_tag="VFL04_01920"
     CDS             complement(3350..4123)
                     /locus_tag="VFL04_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838487.1"
                     /translation="MKRPILAAALSLAALLSLAAASNEGGSPDSGQAGNGSYSEFPTV
                     ESLNLHLKLAGMARASAPAVVDGYLVLSARGPYRFVGAAFAHEGFAAVHAFKKNAKGV
                     FVLAYKVPLKRSEALSYRLVIDGVWTWDPANPRREMDPELGLAVSLADVPYLSDLHLG
                     LYELLDRRDGRTANFIFRAAPGESVTVSGDFDNWDPFIHEMSETSPGVYELSLPLTPG
                     THLYGFVYRGSLVPDPLNVEKASNREGKIVSVISVGQGR"
     gene            complement(4253..>4780)
                     /locus_tag="VFL04_01925"
     CDS             complement(4253..>4780)
                     /locus_tag="VFL04_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838488.1"
                     /translation="VEALEVGELEASRLGLGLGFGFGRRASRGELLGGEGFCGFSGLG
                     LRGLLKGGRLFCLGSCLVEGGQIPEGGEVKCLLLGGSRLSLLGGRGLEELAEILVIEA
                     AGLGGGLLGAGPLLGEGLEQGAELGVGGFGEGFGLGFGRLEGSRFIGARRLDVGRAFA
                     PAFQDFPLVSQRGEI"
BASE COUNT          781 a         1674 c         1598 g          727 t
ORIGIN      
        1 ctggatcgac tcccccgagc cccccgcgag gggctcaatc cccggcgcga aggcgtcacc
       61 gccgaagaag aggctgaaca tggttgcgca gccgagcctc gagccgttgg agaaccactt
      121 cattcggggg taggcctcgc ccagctcctg atgcagaagg gcggcccccg aggcgtggag
      181 gcgctcgggc tcgaggccta cggcctccaa ggtccagatc ggggccccgt cgccccctgt
      241 ccagcggctc cagacgtcct ggtgggggtc gatgaggacg gcgaagcccc actcgcaggc
      301 cttggccacg atggcctcga tgtagtcaag gtactcgtgg tcgtatatcc cgggcccgtc
      361 gtgctcgacc gcctcccagg tgacgaggag cctgaggaag ttcatgcccc agcgctttag
      421 gcgacggaaa tgggcgtcgg cctcggcgag ggggaagggc cttccgacga aggagacgcc
      481 tcgtcgctga tagaagccct ccttgaggtg ggtggcccct tcgggcctcg aggggacctt
      541 gcagtccccg ccgaggttga cccccctgag cacgagcctc cgcccttcct catcccgcca
      601 gctctcgcct attatctgca tggtggcccc ttgaggccga ctatagcccc ggccatagag
      661 cgccgacaat ggagctaggg cggtcccggc gggaccctgc acgagtggag gatcaaggtg
      721 atagacaagg gtgacgtgga ggggcgggtc ctcttcgacg acggggagca caagttcatc
      781 tggctcggga ccgatcaggc cgaccgcaag ggcatcgtcc agaccctcca gtacctggtc
      841 gtggacaagg gccgaggctg gctcctcgat ccgggcgggg tccacctctt ctcccaggtc
      901 gtggcgagcg cgagccgcta catctccctg gacaggatcg aggggatctt cttctcccac
      961 caggaccccg acgtctcctc gggcatagcc ctctggctcg gggtcacctc ggcgaaggtc
     1021 tacatctcca gcctctggac gaggttcatc ccccatttcg gcctcgtcga ccagaccagg
     1081 atcacggcga tcgaggacat gggcaaggcc ctggtcctgg gctcgggagc gaggctgagc
     1141 ttcgtcccca cccacttcat gcactcgccg ggggcctggt ccctcttcga cgagcgtgcg
     1201 aggatcctct tctcggccga cgtcggtgcc gccgtcttcc ccgagggtca ggagaccctc
     1261 ttcatcgacg acttcgccac agtcctcccc ttcaccgagg gcttccacaa acgctatatg
     1321 gcgtccaacg ccgtggtgcg cagatgggcc gacatcgtcc gccgcctcga gccccagatg
     1381 atcgcgcccc agcacggggg catctaccgg ggcagggccg tcggagactt cctctcctgg
     1441 ctgtcctcgc tgcgctgcgg cgtcgacctc ctcgacgagc tgtaccggat ctgaggcgca
     1501 catgcaggaa gacatcctct ccaatttcag ggtggcgctt gaccgcttcg ccatcggcta
     1561 caacaagtcc gtcgtcctct cgtcggtgac ccagcgctcc ctcggcatcc tcgacacgtc
     1621 gatgcggggc atcgaggagt ccctctccac catcgtcgcc gccttcgagg agatacgggc
     1681 cacgggctcg agcacctcag agaacgccgt gaggatcgac gagatgatgt cggcgatcct
     1741 cgtcaagaac gcgagcatgg actccgacgt cgtcgagcgc atggacgaca tcgagcgcgc
     1801 ggccagcgac gcccggcaca tcgacggcct gttccgcgag ctccagggca agaccaagag
     1861 catcgagaac gtgaccgggg cgatacgcga cgtctccgac cgcacgaaca tcctcgcgat
     1921 caacgcctcc atcgaggccg cccgggctgg ctcggtggga aagggcttca ggatcatcgc
     1981 gaacgaggtg aggaacctcg cgggccagac cggggacttc gcgaaggaga tcgagtcgac
     2041 catcggcgag ttccgctcct cggtcgcggt gatcgacgag cgcatgcgcg gcttcatcga
     2101 gctgctcgag cgcttccgct cctccttcgc cgagatcctc gccaacttca aggcgaacgc
     2161 cgtcagcatc gacgaggctg gcaacttcct cacccagatc gcggcggcca tccaggagga
     2221 gacgaaggcc ctctccgagg gcctgggctc cctcgagcag atctcggcca acgtgaagga
     2281 cacccacgca gtcttcagcg ccctctcgag cagccacgcc ttcctcgaca ccctcctcga
     2341 caagggagag gcctgaaggt ggaagtgggc ggagcggggc cccgccgccc cctggcggtc
     2401 atcacggacg accgcttcgg cgagtccgcg atcgaacagg ccatcctcgg ggcggccgga
     2461 gtcgagctcg tcgtggccaa gtgccggagc tcggccgagg tcgcggccgc gggccgggag
     2521 gccgacgccc tcctcgtgaa cctcgccccc gccgacgccg cggccatcga ggccctcgag
     2581 cggtgcaggg tcatcgcccg ctacgggatc ggcctcgaca acgtggacca ggaggcggcg
     2641 cgccgccgcg ggatcagggt ggccaacgtc cccggctact gcgacaccga ggtggccgag
     2701 cacgccctcg ccctgatcct cgccctctcg cgcgggatcg ccaggcggga ccgcgtcgtg
     2761 cgcggcggcg gctggggggg gacacccttc ggccggcgga tcgcgggcac tacgctcggc
     2821 atcctcggct tcggcggcac gggccaggcc ctggcgagga tagccctcgc cctctccttc
     2881 cgcgagatcc tggtctggag cccccacatc agcgccgggc gcatcgccga ggccctgggc
     2941 cctgcccccg ccgccttggg cgcggtggtc aggccggcgg gcttcgagga gctcctgtcg
     3001 cgttcagact gggtgtcggt gcatctgccg ctcaagggcg agacccgcgg catcgtcggg
     3061 gcctcggcca tggccatgat gaaaaaggaa gcctgtctcg tgaacgtgtc ccgcggggcc
     3121 gtgctggacg aggaggccct catcgacgcg atggccacgg gggccctcgc cggggtcggc
     3181 ctcgacgtct tcgtggccga gcccctgccc cagggcaaca ggctccgctc cttccccaac
     3241 gccgtcttca ccgaccacaa cgcctacgcc tcgaccgagt cgatcgccga actcagacgc
     3301 aggacagccg agaacgccct cgccggcctt gccgccgccg gcctcgtgcc tagcggccct
     3361 ggcctaccga gatgactgag acgatcttgc cctcgcggtt cgaggccttc tcgacattca
     3421 gggggtcggg gacgagggag ccccggtaca cgaagccgta gaggtgggtc cccggggtca
     3481 ggggcagcga gagctcgtac accccgggcg atgtttcgct catctcgtgg atgaaggggt
     3541 cccagttgtc gaagtcgccg ctcacggtga ccgactcccc cggcgcggcg cggaagatga
     3601 agttggccgt cctgccgtcg cgccggtcga ggagctcgta gagcccgagg tggaggtcgc
     3661 tcaggtaggg cacatcggcc agggagacgg ccaggccgag ctcggggtcc atctcgcggc
     3721 gggggttggc gggatcccag gtccagactc cgtcgatgac gaggcggtag gagagggcct
     3781 cgctccgctt caggggcacc ttataggcaa gcacgaagac gcccttcgcg ttcttcttga
     3841 aggcgtgcac ggcggcgaag ccctcgtggg cgaaggccgc gcccacaaag cggtacgggc
     3901 ccctagccga caggacgagg tagccgtcga cgacagcggg ggccgaggcc ctggccatgc
     3961 cggccagctt caggtgaagg ttgagggact cgaccgtagg gaactcggaa tagctgccgt
     4021 tccccgcctg gccggagtcg gggctcccgc cctcgttgga ggctgcggcg agggaaagca
     4081 gggcggcgag gctcagggcc gccgcaagga tgggtcgttt catggcgatg tctcgaatat
     4141 cggcagcttc cgagccctcg ctcaagcgga tttccttcac aaagcgccat aacgtccgtt
     4201 aatataggga aggatcccta tgccccgtct gcaggacatc gaacgcttca aactagatct
     4261 cgcctcgctg gctcacgagg gggaagtcct ggaacgctgg ggcgaaagcc ctccccacat
     4321 cgagcctccg ggcgccgatg aagcggctcc cctcgagcct gccgaagccg agtccgaatc
     4381 cttccccgaa tccgccgacg ccgagctcgg cgccctgctc gagcccttcc ccgaggaggg
     4441 ggccggcgcc gaggaggccc ccgccgaggc cagcggcctc gatgaccagg atttcggcga
     4501 gctcctcgag cccccgccct ccgaggaggc tgagacggct cccgccgagg aggaggcatt
     4561 tgacctctcc tccttcgggg atttggcccc cttcgacgag acagctcccg aggcagaaga
     4621 gccttccccc cttgaggagg ccccgcagcc cgaggccgga gaagccgcag aagccttccc
     4681 cgccgaggag ttcgcccctg gaggcccgcc gcccgaagcc gaagccgagg ccgagcccga
     4741 ggcgggaagc ttcgagctcc ccgacctcga gggcttcgac
//
LOCUS       DASSQV010000110         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2142717, whole genome shotgun sequence.
ACCESSION   DASSQV010000110 DASSQV010000000
VERSION     DASSQV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142717"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFL04_01930"
     CDS             complement(<1..353)
                     /locus_tag="VFL04_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7838489.1"
                     /translation="MDNTTVYLVIVALAAWLLVSRLGGVKKAPSAVTAQKIASGARVI
                     DVRSRSEFASGAYRKAKNIPLDEIAGRLGELGPKDGAIVVYCASGARSSKAARILMKA
                     GFGDVTNAGGLGAMP"
     gene            474..995
                     /locus_tag="VFL04_01935"
     CDS             474..995
                     /locus_tag="VFL04_01935"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7838490.1"
                     /translation="MKDALIAIAEYNRNANLRLVGILEGLSPAVLKEDQGSFYKSIQA
                     TLEHICMAEISWLRRFKGFFSYPSLAASATLASENAELKQRVGDDPKKLFALLAEVDG
                     LLAAFAGELNEKDLESRVKYKNIKGEELERTYWHTIFHILNHSTHHRGEISALLDRKG
                     VANDYAGFTLYTK"
     gene            complement(1092..2129)
                     /gene="dapA"
                     /locus_tag="VFL04_01940"
     CDS             complement(1092..2129)
                     /gene="dapA"
                     /locus_tag="VFL04_01940"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET7838491.1"
                     /translation="MMRLSGCYTALVTPFRPDGEVDYEGLRRLVDFQFSQGIDGILAC
                     GTTGESPTLDWSEHNKVTERVHEHSRGRGITIAGTGSNSTQEAIDGTVHAAHIGVEAV
                     LLVDPYYNGPSSLEIRLEYVEPIARAFPKTLVIPYVIPGRTGTQLLPQDLAQLADAFP
                     NVRSVKEATGDLDNMRLTRELCGKDYDILSGDDDKTFTMMTDPRIAASGVISVSSNVA
                     PKAVGDLVRRVRTGDIEGARSLAAALKPLFEVITVKTVEKSPYGPVACRARNPLATKT
                     LMNLLGMPSGPCRAPLGRMTRAGLELVVQAARRVNEANPEILGPVGEFFGVDLSARLY
                     DGKVLEGLCYA"
BASE COUNT          362 a          778 c          810 g          362 t
ORIGIN      
        1 aagggcatcg cgccgaggcc gccggcgttg gtcacgtcgc cgaagccggc cttcatgagg
       61 atccgggcgg ccttggagct gcgagccccc gaggcgcagt agaccacgat cgctccgtcc
      121 ttggggccaa gctcgccgag ccggcccgcg atctcgtcca gcgggatgtt cttggccttc
      181 ctgtaggcgc ccgaggcgaa ctcgctccgg gacctgacgt cgatgacgcg ggcgccggag
      241 gcgatcttct gcgccgtgac ggcggagggg gccttcttga cgcccccgag ccgcgagacg
      301 agcagccagg ccgcgagggc cacgatcacc agatagacgg tagtgttgtc catggctccg
      361 atggtaggcg aacccgcggt ccggagtctg tgacccgggt cacactggaa gcggccccgg
      421 ccggcagacc acgggggccg atcctgctat actgccgcga gatggaggct gcaatgaaag
      481 acgcgctgat cgcaatcgcg gagtataacc ggaacgccaa cctgcgcctg gtcgggatac
      541 tggagggcct gagccccgca gtgctcaagg aggaccaggg ctccttctac aagagcatcc
      601 aggccaccct tgagcacatc tgcatggccg aaatcagctg gctcaggcgt ttcaagggct
      661 tcttctccta ccccagcctg gccgcctcgg ccaccctggc ctcagagaac gccgagctca
      721 agcagcgggt cggcgatgac cccaagaagc tcttcgccct cctcgccgag gtggacggcc
      781 tcctcgccgc cttcgccgga gagctgaacg agaaggacct ggagtcccgg gtcaagtaca
      841 agaacatcaa gggcgaggag ctcgagcgca cttactggca cacgatcttc cacatcctca
      901 accattccac ccatcaccgc ggcgagatct cggcccttct ggaccgcaag ggcgtcgcga
      961 acgactacgc gggcttcacc ctctacacca agtaggccgc atggcgggcc ctgccccgga
     1021 tccccggggc aggggcccga gaccggaccg ccgcggcggc gctccgccag gccccggcgg
     1081 gcgggccgcg cctaggcgta gcagaggccc tcgaggacct tcccatcata gagccgcgcc
     1141 gacaggtcga cgccaaagaa ttccccgacg ggcccgagta tctcggggtt cgcctcgttg
     1201 acccgccgcg ccgcctggac cacgagctcg agcccggcgc gggtcatcct gcccaaaggc
     1261 gcgcggcagg gccccgaggg catcccgagc aggttcatga gggtcttggt ggccagggga
     1321 ttgcgcgccc tgcaggcgac ggggccgtag gggctcttct ccacggtctt gacggtgatg
     1381 acctcgaaca ggggcttaag ggccgcggcc aggctccgcg ccccctcgat gtccccggtg
     1441 cggaccctgc gcacgaggtc cccgacggcc ttgggggcca cgttgctcga caccgagatc
     1501 acgccgctgg ccgcgatcct cgggtccgtc atcatcgtga aggtcttgtc gtcgtccccc
     1561 gagaggatgt cgtagtcctt gccgcagagt tcgcgggtga gcctcatgtt gtcgaggtcg
     1621 ccggtggcct ccttgacgct gcggacgttg gggaaggcgt cggcgagctg ggccaggtcc
     1681 tggggaagga gctgggtgcc cgtccgccct gggatcacgt aggggatgac gagggtcttg
     1741 gggaaggccc tcgcgatggg ctcgacgtac tcgaggcgga tctcgaggct cgaggggccg
     1801 ttgtagtagg ggtcgacgag gaggaccgcc tccaccccga tgtgggccgc gtgcacggtg
     1861 ccgtcgatgg cctcctgggt ggagttgctg cccgtgccgg cgatcgtgat gcccctgccc
     1921 cggctgtgct cgtggacgcg ctcggtgacc ttgttgtgct cgctccagtc cagggttggg
     1981 ctctcacccg tggtgccgca ggcgaggatc ccgtcgatcc cctgggagaa ctggaaatcc
     2041 acgagtctcc gcaggccctc gtagtccacc tcgccgtccg ggcggaaggg ggtcacgagg
     2101 gccgtgtagc aaccgcttaa gcgcatcatc tgggcagctc ctccgaaacg cgcgagacgt
     2161 ttctctgggc agaagctact cgctgttgag ccggcggtca atgcgcggtg cgggggaagg
     2221 gcggggaatg caggccgggg agggccgggc gcggcccgcg cgggccaggc tgcgggcgcg
     2281 gcccccccgc ccgctagaag gttatctcct tc
//
LOCUS       DASSQV010000111         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_525810, whole genome shotgun sequence.
ACCESSION   DASSQV010000111 DASSQV010000000
VERSION     DASSQV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525810"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2301)
                     /locus_tag="VFL04_01945"
     CDS             complement(<1..>2301)
                     /locus_tag="VFL04_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7838492.1"
                     /translation="AYKARLDSTPSIGLLVTKTSDANVVETAKRVKAALEAERRDLPP
                     GMDAKTASDATVFIEASLSETRRDLLLGILATSAVLFLFLRKWRSSLIVLVAIPSSLV
                     ATFFMMYMSGFTLNILSVMALALCIGILVDDSIVVLENIHRHRGLGEDPLRAAISGRT
                     EIGLAAIAITLCDVVVFAPVAFMGDIVGQFFRQFGLTVVFASLFSLLVSFTLTPAMAS
                     RFLESPSREGMPDEGGRPRGRLNGLFEVRIKAAYRRLLEWSLSHRWRVVGLVAALFAS
                     SLALIPLGLIQTEFMPPFDQGKLVIDLRLGAGSELRSTEAAVGLVEAQLRGMDEVADV
                     FSQIGTSTGGTGSANLVVRLKERGLRKKTQAALAKELRVWAAGLPSLSASVSEPAIVA
                     QTSIEGKKALILNVSGPDRAVLARIADGIEAALRSIPGAVDVENSIDSRHTTIGVGLD
                     RLALSEYGLSASDAALALRTALAGTKAGVYRKAGSEYDIVVRLRPDQMRKPGDIGDLR
                     LLGRTGAMVPIDQVAALHREDSASSLERRNRSNVVTIQANLEGRPLGAVTAELRSRLA
                     ALPMPSGYGYAFAGDVSNMSSSFGSLAWALAASIGLVYLILLALYESYLTPAIRMLSL
                     PAGIIGGLGALALSGKAINIVSFIGIIMLDGLISKNGTLLIDYTHTLMRRGLQLREAL
                     LEAATTRLRPILMTSFTMIAGMLPLALSGGSSSEIKSGMAIVLIGGLSTSTLISPLLL
                     PVAYTLIDDARQRRRAEAPGDDGGPK"
BASE COUNT          389 a          700 c          853 g          359 t
ORIGIN      
        1 gcttcggccc cccatcgtcc ccgggagcct cggccctcct cctttggcga gcgtcgtcga
       61 tgagggtata ggccacgggg aggaggaggg gggatatcag ggtcgaggtg gagagacctc
      121 cgatgagcac gatggccatg cccgacttta tctcggagga ggagcccccc gagagggcga
      181 ggggaagcat gcccgcgatc atggtgaagg aggtcatgag tatgggcctg agcctcgtcg
      241 tggccgcctc gaggagggcc tcgcgcagct ggagcccccg cctcatgagg gtgtgggtgt
      301 agtcgatgag gagggtcccg ttcttggaga tcaggccgtc gagcatgatg atgccgatga
      361 aggacacgat gtttattgcc tttcccgaga gcgcaagggc tccgaggccg cctatgatgc
      421 ccgcaggcag ggagagcatc ctgatggcgg gggtgaggta ggattcgtag agggcaagga
      481 ggatcaggta gacgaggcct atggaggcag cgagggccca ggccagggag ccgaaggagc
      541 tcgacatgtt ggagacgtca ccggcgaagg cgtagccata gcccgatggc atgggaaggg
      601 ccgcaagccg ggacctgagc tcggcggtca cggccccgag gggcctgccc tcgaggttgg
      661 cctgtatcgt gaccacgttc gagcggttgc ggcgctcgag gcttgatgcc gagtcctccc
      721 tgtgcagggc ggcgacctgg tcgatcggca ccatggcccc cgtcctgcca aggagcctca
      781 ggtctccgat gtccccgggc ttcctcatct ggtcgggcct gagcctgact acgatgtcgt
      841 actcgctgcc cgccttccgg tagaccccgg ccttggtgcc cgcgagggcc gtcctgaggg
      901 cgagggcggc gtcgctcgcc gagagcccgt attcgctcag ggccagcctg tccagaccga
      961 cgccgatggt ggtgtgtctc gagtcgatgg agttctcgac gtcgacggcc cccgggatcg
     1021 agcgcagcgc ggcctcgatg ccgtcggcga tccgcgcgag cacggcgcgg tccggccccg
     1081 acacgttgag tatgagggcc ttcttcccct cgatcgaggt ctgggccacg atggcgggct
     1141 cgctgaccga ggcgctcagc gagggaaggc cggcggccca cacgcggagc tccttcgcga
     1201 gcgccgcctg ggtcttcttc ctcaggcccc tctccttgag cctcacgacg aggttggccg
     1261 agcccgtccc gcccgtcgag gtcccgatct ggctgaacac gtcggccacc tcgtccatcc
     1321 cgcgcagttg ggcctcgaca aggccgacgg ccgcctcggt gctccggagc tcagacccgg
     1381 cgccgagcct caggtcgatg acaagcttgc cctgatcgaa gggcggcatg aactcggtct
     1441 ggatgagccc cagggggatg agggcaaggc tggacgcgaa gagggcagcc accaggccga
     1501 cgacccgcca tcggtgcgag agcgaccact cgaggaggcg gcggtaggcg gccttgatcc
     1561 tcacttcgaa aagcccgttg agccgtcccc ggggccgccc cccctcgtcg ggcatcccct
     1621 cccggctcgg ggactccagg aagcgggagg ccatcgccgg ggtcagggtg aaggagacga
     1681 ggagggagaa cagggacgcg aagacgacgg tgaggccaaa ctgtctgaag aactggccca
     1741 cgatgtcgcc catgaaggcg acgggggcga agacgacgac gtcgcagagg gttatcgcga
     1801 tggccgcgag ccctatttcc gtccgccccg agatggcggc gcggagggga tcctcgccga
     1861 ggccccggtg gcggtggatg ttctcgagga cgacgatcga gtcgtcgacg aggatgccga
     1921 tgcagagggc gagggccatc acggagagga tgttcagtgt gaagccgctc atgtacatca
     1981 tgaagaaggt cgcgacgagg gagctgggga tggccacgag gacgatcagg gatgagcgcc
     2041 atttccgcag aaagaggaag aggacggccg atgtggctag gatgccgagc aggaggtcgc
     2101 gtctcgtctc ggagagggag gcctcgatga acaccgtggc gtccgaggct gtcttcgcgt
     2161 ccatgccggg tggcaggtcc cgccgctccg cctcaagggc cgccttgacg cgcttggcgg
     2221 tctcgacgac gttggcgtcg ctggtcttgg tgaccagaag cccgatcgag ggcgtggagt
     2281 cgaggcgcgc cttgtacgcc t
//
LOCUS       DASSQV010000112         5642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1395854, whole genome shotgun sequence.
ACCESSION   DASSQV010000112 DASSQV010000000
VERSION     DASSQV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395854"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VFL04_01950"
     CDS             complement(<1..751)
                     /locus_tag="VFL04_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7838493.1"
                     /translation="MLDIIRLPRGAALAAEAAREARSFAVGFWFPLGSRHEAAHERGF
                     VHFVEHMAFKGTARRSAVALSREIDRVGGYLNAFTDRDSICLHCLVPASSWRLALDVL
                     SDMAFSSLFAEEDFARERDVIVSEILSARDDPEECSHEELLARIWPGDPLSRKIAGEP
                     EDIERATRGALYEFYRSSLVPGSLLVTAAGPVEPDELAEELGRILGDQGLGARPPARP
                     RPSAVPRFVPARVYRRAAMEQVNYFEAVQLDP"
     gene            complement(754..2994)
                     /gene="pnp"
                     /locus_tag="VFL04_01955"
     CDS             complement(754..2994)
                     /gene="pnp"
                     /locus_tag="VFL04_01955"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET7838494.1"
                     /translation="MAHKLSYRIGNEELVLETGRMAKQANGAVFATFGGSAIIATACC
                     SDKVTEGLDFVPLTVEYNEKFYAAGKIPGGFIKRETRPKDREILVSRIIDRPMRPLFD
                     KAFGREIQVVPTCISFDGVNPPDILGLIAASAAVTISDIPFSGPIAGVRVCLVDDEYV
                     INPTHDQIQRSKLEIVVAGTAEGITMVEGHASEASEEEMLKAIEASKKPIADICAMIV
                     KLRELAGKDKLGLAPLKVVLNRKAEIEAHARGLLQTALFTKIKHERAAAVSKAKKDTA
                     AKFADALTDEVQKKLYDALIEDLCYEILRSGILDKGMRVDGRGTEDIRPITCEISVLP
                     RTHGSALFTRGETQALGVTTLGTVFDEQILDDIEGDRRDRFMLHYNFPPFSVGEVGRL
                     GTGRREIGHGHLARRAVEEVLPTKEAFPYTVRVVSEVLESNGSSSMATVCAATLSLLQ
                     AGVPLKMPVAGIAMGLITDGSRYAVLSDILGDEDHLGDMDFKVAGSTRGITAFQMDIK
                     IAGVSSEILTKALDQAKRGRMHILGIMEKTISVPAAEISIYAPKVVSTKVDVEKIGAI
                     IGPGGKNIKALCEMFNVKINVDDDGSVTIYGSTQKAAYDAKDAIMGIVEDPEVGRIYE
                     GTVKRIMDFGAFIEILPGKEGLCHISRLAKTRVERVSDVVKEGDKVKVKLLEVDRLGR
                     LNLAHVDSLDENGNSPSPEHSDRGERPSYGDRPSYGDRPRGDRPHGGSDRGDRGGPRR
                     DDSRRR"
     gene            complement(3117..3386)
                     /gene="rpsO"
                     /locus_tag="VFL04_01960"
     CDS             complement(3117..3386)
                     /gene="rpsO"
                     /locus_tag="VFL04_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET7838495.1"
                     /translation="MALTKADKARVVLEHGKSEKNTGSTETQIALLTERITMLTEHFK
                     VHKKDTNSRRGLLMLVGQRRRLLKYMMRTDLAGYRVLIEKLSIRK"
     gene            complement(3402..4208)
                     /locus_tag="VFL04_01965"
     CDS             complement(3402..4208)
                     /locus_tag="VFL04_01965"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HET7838496.1"
                     /translation="MKVVDWEDFAPDPDGLPLAAAIGVFDGLHLGHRALIDLVLSRGG
                     MRRGVVTFRENPKKLLRPASFHGNLSTLSQKLELLESMALEICVLIDFSGDFSRMAGR
                     KFLSLLRERGDLRYLAVGSDFRCGHRLDTGAHELREFFVAQGVEADILDPVLRTGHPV
                     SSSRIRKAVVDGRLEDARGMLGRDYEIDLRGCRPGSGGNGSLHFAPGGVQVAPPAGLY
                     EAELVGPDGRLATRAAFDGKGWEVEAGPGDGAAFPAEPVALRVVRSVSTE"
     gene            complement(4205..5122)
                     /gene="truB"
                     /locus_tag="VFL04_01970"
     CDS             complement(4205..5122)
                     /gene="truB"
                     /locus_tag="VFL04_01970"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET7838497.1"
                     /translation="MRPASGHEPRSGIALLRKEVGLSSFQTLSPVKRRVGGAKVGHAG
                     TLDRFAEGLLVALVGQYTRLVPYVVPGEKTYRGVVRFGAETSTLDPEGEVIARAPVPG
                     LGELEAALPRFIGHILQKPPAYSALHIDGVRAHRRARAGEEIDMPARPVEVYECRLES
                     FEGADATILVRCSAGTYIRSLARDIAIACGSRAHLVALDRRAIGPFPVEDAVGAEDFD
                     PDRDLLSLDAEKAAALGLPAFSLCEALMPTFMNGVALRPEDYRSLGSGAPLAEGLPSA
                     VFAGGGRIIGIVELGQGRVHYRAVLGGGA"
     gene            complement(5119..5541)
                     /locus_tag="VFL04_01975"
     CDS             complement(5119..5541)
                     /locus_tag="VFL04_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HET7838498.1"
                     /translation="MDEIRRRRVEEQIRDEIAALIMGGDVKDPRIGPFVSVTRVEAAR
                     DLSTARVFVSSFASVAAPAPAGGEPAAAPAAAEAPVHEADPALDRAVAGLQSAAGYIQ
                     AQVARRIKLRLTPRLHFIPDRGIKDGFELNERIKGLFS"
BASE COUNT          829 a         1851 c         2014 g          948 t
ORIGIN      
        1 gggggtcgag ctggacggcc tcgaagtagt tgacctgctc catcgccgcg cgtctgtaga
       61 cccttgcggg gacgaagcgc ggcacggccg agggccgggg cctggccggg ggccttgcgc
      121 cgaggccctg gtcgccgagg atgcggccga gctcctcggc cagctcgtcc ggctccaccg
      181 gccccgccgc cgtgaccagg agggatccgg gcacgaggct ggacctgtag aactcgtaga
      241 gggcgccccg ggtcgcgcgc tcgatgtcct cgggctcccc ggcgatcttc ctcgagagcg
      301 ggtcgcccgg ccagatccgc gcgaggagct cctcatgcga gcactcctcg gggtcgtccc
      361 gcgccgagag gatctcgctc acgatcacgt ccctctccct ggcgaagtcc tcctcggcga
      421 agagggagga gaaggccatg tccgaaagga cgtcgagggc gagcctccag ctcgaggcgg
      481 ggacgaggca gtggaggcat atggagtccc ggtcggtgaa ggcattcagg tagccgccga
      541 ccctgtcgat ctcgcgagag agggcgacgg cgctcctgcg cgccgtgccc ttgaaggcca
      601 tgtgctcgac gaagtgcacg aagccgcgct cgtgcgcggc ctcgtgtcgt gagccgagag
      661 ggaaccagaa tccgacggcg aaggatctcg cctcccgggc ggcttcggcc gcgagggcgg
      721 cgccgcgggg gaggcggatt atgtccagca cgcctagcgc ctccgggaat cgtcccttcg
      781 cgggccgccg cgatcaccgc gatcgctccc tccgtggggg cggtcgccgc gcgggcggtc
      841 gccgtacgac gggcggtcgc cgtaggaggg gcgctcgccg cggtcgctgt gctcggggct
      901 ggggctgttg ccgttctcgt cgagggaatc gacgtgggcg aggttgagcc tgccgaggcg
      961 gtcgacctcg agcagcttga ccttcacctt gtccccttcc ttgacgacgt cgctgacccg
     1021 ctcgaccctg gtcttggcga gccgggagat gtggcagagc ccctccttgc cggggaggat
     1081 ctcgatgaag gctccgaagt ccatgatgcg cttgacggtg ccctcgtaga tccggcccac
     1141 ctcggggtcc tccacgatgc ccatgatggc gtccttcgcg tcataggcgg ccttctgggt
     1201 cgagccgtag atggtcaccg agccgtcgtc gtcgacgttg atcttgacgt tgaacatctc
     1261 gcagagcgcc ttgatgttct tgccgcccgg cccgatgatc gcgccgatct tctccacgtc
     1321 gaccttggtc gagacgacct tgggcgcgta gatggagatc tcggccgcgg gcacggagat
     1381 ggtcttctcc atgatcccga ggatgtgcat cctgccgcgc ttggcctggt cgagggcctt
     1441 ggtgaggatc tcgctggaca cgccggcgat cttgatgtcc atctggaagg ccgtgatgcc
     1501 gcgggtggag ccggcgacct tgaagtccat gtcgccgagg tggtcctcgt cgccgaggat
     1561 gtccgaaagc acggcgtagc gggagccgtc ggtgatgagg cccatggcga tgccggccac
     1621 gggcatcttg agcgggacac cggcctggag gagggagagg gtcgcggcgc agaccgtggc
     1681 catcgacgag gacccgttcg actcgaggac ctcggagacg acgcggacgg tgtaggggaa
     1741 ggcctccttg gtcgggagga cctcctcgac ggcgcggcgc gcgaggtggc cgtggccgat
     1801 ctcgcggcgg ccggtgccga gccgccccac ctcgccgacc gagaagggcg ggaagttgta
     1861 gtggagcatg aagcggtcgc gcctgtcgcc ctcgatgtcg tcgaggatct gctcgtcgaa
     1921 gacggtgcca agggtggtca cgccgagggc ctgggtctcg ccccgcgtga acagggccga
     1981 gccatgggtg cggggcagga cggagatctc gcaggtgatg ggcctgatgt cctcggtgcc
     2041 gcggccgtcg acgcgcatgc ccttgtccag gatgccgctg cggaggatct cgtagcagag
     2101 gtcctcgatc agggcgtcgt agagcttctt ctgcacctcg tccgtcagcg cgtcggcgaa
     2161 cttggcggcg gtgtccttct tggccttcga caccgcggcg gcgcgctcgt gcttgatctt
     2221 cgtgaagagg gcggtctgga ggaggccgcg ggcatgggcc tcgatctcgg ccttgcggtt
     2281 gagcaccacc ttgagggggg cgaggccgag cttgtccttg ccggcgagct cgcggagctt
     2341 gacgatcatc gcgcagatgt ccgcgatggg cttcttcgag gcctcaatgg ccttgagcat
     2401 ctcctcctcg gaggcctcgg aggcgtggcc ctcgaccatc gtgatcccct cggcggtgcc
     2461 ggccacgacg atctcgagct tggacctctg gatctggtcg tgggtggggt tgatgacata
     2521 ctcgtcgtcg acgaggcaga cgcgcacgcc ggcgatcggg cccgagaagg ggatgtcgga
     2581 gatcgtgacc gccgcgctcg ccgcgatgag gccgaggatg tcgggcgggt tcaccccgtc
     2641 gaaggagatg caggtcggca cgacctggat ctcgcggccg aaggccttgt cgaagagggg
     2701 ccgcatgggc ctgtcgatga tgcgcgagac caggatctcg cggtccttcg ggcgggtctc
     2761 gcgctttatg aagccgccgg ggatcttccc cgccgcgtag aatttctcgt tgtactcgac
     2821 ggtgaggggg acgaagtcga ggccctcggt caccttgtct gaacagcagg cggtggcgat
     2881 gatcgcgctc ccgccgaagg tcgcgaatac cgcgccgttg gcctgcttgg ccatgcgacc
     2941 ggtctcgagg acgagttcct cgttgccgat cctgtatgaa agcttgtgcg ccatgtcttc
     3001 cttcttgtta tgcgccaata cgaataggcc cgtgccttga cggccgggcc tcctgatccg
     3061 gtgcgcgccg ccgcgatggt cggccacccc ggacgtcgcc cctcagggcg acctggtcac
     3121 ttgcggatac tgagcttctc gatgagtaca cggtagccgg ccagatccgt ccgcatcatg
     3181 tacttgagca ggcgtcggcg ctggccgacg agcatgagca ggccgcgccg cgagttggtg
     3241 tccttcttgt ggaccttgaa gtgctcggtg agcatggtga tgcgctcggt gaggagggcg
     3301 atctgggtct cggttgagcc ggtgttcttc tcgctctttc cgtgctcgag cacgactcgg
     3361 gccttgtccg ctttggtcaa cgccatctta tctatctcct tctactctgt tgataccgat
     3421 cgtacgacgc ggagcgccac gggctccgcg gggaaagccg ccccgtcccc gggcccggcc
     3481 tccacttccc atcccttgcc gtcgaaggcg gccctggtcg ccagccgacc atcgggtccc
     3541 acgagctcgg cctcgtagag cccggcaggc ggggccacct gaaccccgcc cggagcgaag
     3601 tgcagcgacc cgttcccgcc cgagcccggc ctgcagcccc gcaggtcgat ctcgtagtcc
     3661 ctgccgagca tgccccgggc gtcctcgagc ctgccgtcca cgacggcctt cctgatgcgg
     3721 ctcgagctga cgggatggcc cgtccggagc accggatcga ggatgtcggc ctcgacccct
     3781 tgagccacga agaactcgcg cagctcatgg gcgccggtgt cgagccggtg accgcaacgg
     3841 aaatcggagc ccaccgcaag gtagcggagg tcgccgcgtt cgcgaagcag ggaaaggaat
     3901 ttccttccgg ccattctact gaaatccccg gaaaagtcaa tgaggacgca tatctcgaga
     3961 gccatcgact cgagcagctc gagcttctgg gacagggtcg acaggtttcc gtggaagctc
     4021 gcggggcgca ggagcttctt ggggttctcc ctgaaggtga cgacccccct cctcatccct
     4081 ccgcggctga ggaccagatc gatgagggcc cggtggccga ggtggagtcc gtcgaagacc
     4141 ccgatcgccg ccgcgagggg aaggccatcg ggatcggggg cgaagtcctc ccagtcgacg
     4201 accttcacgc gccgcccccg agcactgccc ggtagtgcac cctgccctgc cccagctcca
     4261 ctatcccgat gatccggccc cctccggcga acacggccga gggcaggccc tcggccaggg
     4321 gggctcccga gccgaggctc cgatagtcct cggggcggag ggccaccccg ttcatgaacg
     4381 tgggcatgag ggcctcgcaa agcgagaagg ccgggaggcc gagggcagcc gccttctcgg
     4441 catccaggct gaggaggtcc cggtctgggt cgaagtcctc ggccccgaca gcgtcctcga
     4501 ccggaaaggg cccgatggcc ctgcggtcaa gggcgaccag gtgggccctc gagccgcagg
     4561 cgatggcgat gtcccgggcg agggacctga tgtaggtccc cgccgaacag cgcaccagta
     4621 tggtagcgtc ggcgccctca aaggactcga gccggcactc gtagacctcg acgggccgcg
     4681 cgggcatgtc gatctcctcg ccggcgcggg cgcggcggtg ggcgcgcacc ccgtcgatgt
     4741 gcagggccga gtaggccgga ggcttctgga gtatgtggcc gatgaagcgc gggagggcgg
     4801 cctcgagctc cccgaggccg gggacgggag cacgggcgat gacctcgccc tcgggatcga
     4861 gggtgctcgt ctcggcgccg aagcgcacga cgccgcggta ggtcttctcc cccggcacga
     4921 catagggcac aagcctcgtg tactggccca cgagggccac gagaaggccc tcggcgaagc
     4981 ggtcgagggt gcccgcatgg ccgaccttgg cgccgccgac gcggcgcttg accggggaga
     5041 gggtctggaa ggagctgagg ccgacttcct tgcgaagcag ggcgatgccg gatcggggct
     5101 cgtgacccga agcgggtctc acgagaacag gcccttgatc cgctcgttga gctcgaagcc
     5161 gtcctttatc ccacggtcgg ggatgaagtg gagccgaggg gtgaggcgga gcttgatccg
     5221 cctcgccacc tgggcctgta tgtagccggc cgcgctctga agccccgcca cggccctgtc
     5281 gagggcgggg tcggcctcgt ggaccggcgc ctccgcggcg gccggcgcgg ctgcgggctc
     5341 gccgcccgcc ggagccggcg cggccacgga ggcgaagctc gagacgaaga cccgcgccgt
     5401 agaaaggtcc cgggcggcct cgaccctcgt cacggagacg aagggcccga tccgcgggtc
     5461 cttcacgtcg ccgcccatga tgagggcggc gatctcgtcc cgtatctgct cctctaccct
     5521 tctacgcctt atctcgtcca ttctcgctcc ctccgccaac ggcggcgccg agggtgcggg
     5581 ctacctcgat catctcgtat acctcgagga tgtctcccgc ctggagatcg ttgcagttct
     5641 cg
//
LOCUS       DASSQV010000113         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2018673, whole genome shotgun sequence.
ACCESSION   DASSQV010000113 DASSQV010000000
VERSION     DASSQV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018673"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VFL04_01980"
     CDS             complement(<1..832)
                     /locus_tag="VFL04_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET7838499.1"
                     /translation="MGDYFENDAVTKGYDPRIMRRIFGYLRPYRLAVAVTALALIIGT
                     AGELILPVLVRRIVDEALMPSWYAVDPRAGLTREGRGLLLLGAGGASEPILAGRAYLR
                     SSRLSAITGAERRSLEAQGLLDGSPRYLFRLDPGDQAQAAAVSANAERIVSGGGLASM
                     RVDELRSLPAPEARAIRKGDRALLAANASLLFAALVVVLATAFVQTFVANLLGQRIMK
                     DLRMELFRHTATRSLAFLSRQPVGRLVTRLTSDIETINQFFTDVVVAFIKDLSIMVGV
                     V"
     gene            complement(835..>2174)
                     /locus_tag="VFL04_01985"
     CDS             complement(835..>2174)
                     /locus_tag="VFL04_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7838500.1"
                     /translation="VAILAVMLASAPGTALLTFAPLPVITLLIILFGGMVGKRFKRVQ
                     EIYSRLSEIAQETMAGIRVVKAFVKEEHFAQRFSAVNDEYRGASLSLVKIFGFFFPFI
                     SFLAGLTTIILLLAGGAAVIENRMSAGTIVAMLAYLEMLIWPMMGAGFTVNALQRGAA
                     SLKRVNEVLHATPGIASGPGAAAVPPSGDVEFRSLSFAYPGEVEPVLSGIDLRIPKGS
                     TLGILGRVGSGKSTLLWLLPRLIDPPPGTVFIGGHDVRDYELHALRRAFGFVPQDSFL
                     FSDSVENNVRFGAPSLSEERFRLVSEISAIDRDVGLFPSGWSTLVGEKGLTLSGGQKQ
                     RVAIARALAADPEILVLDDALSAVDAETEERILSALLGERSGRTNIIVSHRVSTLRHA
                     DTVIVLEKSGIAQRGTHEELLADEDGFYAEIARLQELEADIRAIERVATGEGD"
BASE COUNT          367 a          693 c          792 g          322 t
ORIGIN      
        1 cgacgacccc gaccataatc gagaggtcct tgatgaaggc gacgaccacg tcggtgaaga
       61 actggtttat ggtctcgatg tcgctcgtca ggcgcgtgac gaggcgcccg accggctgcc
      121 gcgagaggaa ggcgagggag cgggtggccg tgtgcctgaa gagctccatg cgcaggtcct
      181 tcatgatcct ctggccgagg aggttcgcca cgaaggtctg gacgaaggcg gtggcgagga
      241 cgacgacaag ggccgcgaag aggagggagg cgttggccgc gaggagggcc ctatccccct
      301 tcctgatcgc cctcgcctca ggcgccggca ggctccgcag ctcgtcgacg cgcatggaag
      361 cgaggccgcc gcccgagacg atgcgctccg cgttggctga gaccgcggcg gcctgggcct
      421 ggtcgcccgg gtcgaggcgg aaaaggtatc gcgggctccc gtcgaggagg ccctgggcct
      481 cgaggctccg gcgctcggct cccgtgatgg ccgagagccg ggaggacctg aggtaggccc
      541 ttccggccag gatgggctct gaagcgccgc cggccccgag tagcaggagg cccctgcctt
      601 ccctcgtgag ccccgcccgg ggatcgacgg cataccagga cggcatcagg gcctcgtcca
      661 cgatgcgccg gacgagtacc ggcaggatga gctcgcccgc ggtgcctatg atgagggcca
      721 gggccgtcac cgccacggcc agccggtagg gcctgaggta gccgaagatg cggcgcatga
      781 tccggggatc gtagcctttg gtgaccgcgt cgttctcgaa gtagtcgccc acgatcagtc
      841 gccctccccc gtggccacgc gctcgatcgc cctgatgtcg gcctcgagct cctggagcct
      901 ggcaatctcc gcgtagaatc cgtcctcgtc cgccagcagc tcctcgtggg tcccgcgttg
      961 ggcgatgccc gatttctcca ggactatcac cgtatcggcg tgtctcaggg tcgagacccg
     1021 gtgcgacacg atgatgttcg tccgacccga gcgctcgccg aggagggccg agaggatcct
     1081 ctcctcggtc tcggcgtcga ccgcggagag cgcgtcgtcg aggacgagga tctcgggatc
     1141 ggcggccagg gcccgggcga tggccacccg ctgtttctgc ccgcccgaga gggtgaggcc
     1201 cttctcgcct acaagggtcg accagcccga ggggaagagc cccacgtccc tgtcgatggc
     1261 cgagatctcg gagacgaggc ggaagcgctc ctcgctgagc gagggcgcgc cgaagcggac
     1321 gttgttctcg acggagtcgg agaagaggaa ggagtcctgg ggcacgaagc cgaaggccct
     1381 gcggagggca tggagctcgt agtccctcac gtcgtggccg ccgatgaaca ccgtgcccgg
     1441 aggggggtcg ataaggcggg gaaggagcca gaggagggtg gacttgcccg agcccacccg
     1501 gccgaggatg ccgagggtcg atcccttggg gattctgagg tctatgccgc tgaggacggg
     1561 ctccacctcc cccggatagg cgaagctcag ggaccggaac tccacgtccc cgctgggggg
     1621 gacggctgcc gctccgggac cgctcgcgat gccgggcgtc gcgtgcagga cctcgttcac
     1681 ccgcttgagg ctcgcagccc cgcgctggag ggcattgacc gtgaagccgg cccccatcat
     1741 cggccagatc agcatctcga ggtaggcgag catggccact atcgtgcccg cgctcatccg
     1801 gttctcgatc acggccgccc ccccggccag caggaggatg atcgtggtga ggcccgcgag
     1861 gaaggagatg aagggaaaga agaagccgaa gatcttcacg agggagagcg aggctcccct
     1921 gtactcgtcg ttgaccgcgg agaagcgctg ggcgaagtgc tcctccttca cgaaggcctt
     1981 caccaccctg atgccggcca tcgtctcctg ggcgatctcg gagagccggg aatagatctc
     2041 ctgcacgcgc ttgaagcgtt tgccgaccat gccgccgaac aggatgatga ggagggtgat
     2101 gacgggcagg ggcgcgaaag tgagaagtgc ggtgcccggc gcgctcgcga gcatgacggc
     2161 gaggatggcg accg
//
LOCUS       DASSQV010000114         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_899778, whole genome shotgun sequence.
ACCESSION   DASSQV010000114 DASSQV010000000
VERSION     DASSQV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899778"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VFL04_01990"
     CDS             complement(<1..1158)
                     /locus_tag="VFL04_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838501.1"
                     /translation="MTRLLFPAVALLALVLPIRALDWGGSLEDDSAANLGLAKSSSDS
                     VVQTQTLRLWAVQNFGPTANLLAKANVSDALTWNLHPSALSNTVTADLDALVFSSGGL
                     TLGRTTFHDFGSTVLNTTLDGIQYSFGAHGVGYTLAAGSSALVFKSGSTVVISEADLN
                     ERTVAEDWSRPSTLFAPRRAMAYLEATLTSLIPGQTIQLAEAFQYDLRTEGVARAGDS
                     HSHFTPGGPTAPVHMSYTGLGLSGRVAGPLYWDLWSYLGLGLSLTPTGPYAQKGTDPS
                     TGAPIPEVLETWRTSYIVNGLGKMDLSLILPGLNDTIVNLGLLVGSWDRDGISPDQNL
                     PTKPQGNSPSLYTGYFGISRTGSALIFNPQPANMAIGQLLYSFKPLGRSRSD"
     gene            complement(1155..1622)
                     /locus_tag="VFL04_01995"
     CDS             complement(1155..1622)
                     /locus_tag="VFL04_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838502.1"
                     /translation="MRPSLLALAGAVLLLVPVWGQAQGPGDNAIIDGILGQEAISWGN
                     AAWLVGRATGAFDEATTPAAAAELAAKAGWGPSGLSAEAPIDAKAYSLILVKALAIPT
                     GLMYGLFPGPRYAFRELIFRRIVPGTLAPDGAVRGEDAMRYLQAAQDWKEAHT"
     gene            1694..>2256
                     /locus_tag="VFL04_02000"
     CDS             1694..>2256
                     /locus_tag="VFL04_02000"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HET7838503.1"
                     /translation="MKKPKGLPEWSIPFAALALVAGLAIAGLSRPLELRIRDLYTRLV
                     PGPLERPELVLVDVDDLAMQRVGQWPWSRDIHAGFLADLRSLGADSLSFDVEFVDRGP
                     VGVTSREREATLAAKTTELEEVFQALGGRQIRLPEAEDYAKTILHDGVYLTARDNDTF
                     LGQAMAVFGRAYTTLNFSLRNDSEDSKA"
BASE COUNT          338 a          726 c          836 g          356 t
ORIGIN      
        1 atcgcttctc gagcgcccca ggggcttgaa ggagtacaga agctggccga tcgccatgtt
       61 ggccggctgg gggttgaaga tgagggccga gccggtgcgg ctgatcccga agtagcccgt
      121 gtagaggctg gggctgttcc cctggggctt ggtggggagg ttctggtcgg gcgagatccc
      181 gtccctgtcc cagctgccca cgaggaggcc gaggttcacg atcgtgtcgt tcaggccggg
      241 caggatgagg ctcaggtcca tcttcccgag cccgttgacg atgtagctcg tcctccaggt
      301 ctccaggacc tcggggatcg gcgcgcccgt ggaggggtcg gtgcccttct gggcgtaggg
      361 tccggtcggg gtcagggaga gcccgagccc gaggtaggac cagaggtccc agtacagggg
      421 gccagcgacg cggcccgaga ggccgaggcc cgtgtagctc atgtggacgg gggccgtggg
      481 gcctcccggc gtgaagtggc tgtgactgtc cccggccctc gcgacgccct cggtcctgag
      541 gtcgtactgg aaggcctcgg cgagctggat ggtctggccg gggatgaggg aggtgagggt
      601 ggcctcgaga taggccatgg cccggcgggg cgcgaagagg gtcgagggcc ttgaccagtc
      661 ctcggccacc gtcctctcgt tgaggtcggc ctcgctgatg accaccgtgc tccccgactt
      721 gaagacgagg gccgagctgc ccgcggccag ggtgtagccg accccgtggg cgccgaagct
      781 gtactggatt ccgtcgaggg tggtgttgag gaccgtggag ccgaaatcgt ggaaggtagt
      841 gcggcccagg gtgaggcccc ccgaggagaa gacgagggcg tcgaggtcgg cggtcaccgt
      901 gttggagagg gcactggggt ggaggttcca ggtcagggcg tcgctcacgt tggccttggc
      961 aagcaggttc gccgtgggcc cgaagttctg gacggcccag agcctcaggg tctgggtctg
     1021 gaccaccgag tcgctcgagg acttggccag gccgaggttg gccgcgctgt cgtcctcgag
     1081 gcttccgccc cagtccaggg cccgtatggg gaggaccagg gcgaggaggg ccaccgccgg
     1141 aaacaggagg cgcgtcatgt gtgggcctcc ttccagtcct gggccgcctg gagatagcgc
     1201 atggcgtcct cgccccgcac tgccccgtcg ggggcgaggg tgccggggac gatcctgcgg
     1261 aagatgagct ccctgaaggc gtagcggggg ccggggaaga ggccgtacat gaggcccgtg
     1321 gggatggcca gggccttcac gaggatgagg ctgtaggcct tcgcgtcgat gggggcctcg
     1381 gccgagagcc cggaggggcc ccagcccgcc ttggccgcca gctccgccgc cgccgcgggc
     1441 gtggtggcct cgtcgaaggc ccccgtggcc cggcccacga gccaggccgc gttgccccag
     1501 gagatcgcct cttggccgag gatcccgtcg atgatggcgt tgtccccggg gccctgggcc
     1561 tgcccccaga caggcacgag gaggaggacg gccccggcga gggcgaggag ggatggtcgc
     1621 actgcagcct ccgactttct aaaatacatt accattctag cccatcattg ccccgcgctc
     1681 tatgattagg tgcatgaaaa agcccaaagg ccttcccgaa tggtcgattc ccttcgcggc
     1741 cctcgccctc gtcgccggtc tggccatcgc gggcctcagc cggcccctag agctcaggat
     1801 cagggacctc tatacccgtc tggtccccgg ccccttggag cggcccgagc tcgtccttgt
     1861 cgacgtcgac gacctggcaa tgcagcgggt gggccagtgg ccctggagca gggacatcca
     1921 cgcgggcttc ctcgccgacc tgcgctccct cggcgccgac tccctctcct tcgacgtcga
     1981 gttcgtggac cggggcccgg tcggggtcac gagccgggag cgcgaggcca ccctcgccgc
     2041 caagaccacc gagctcgagg aggtcttcca ggccctgggc gggaggcaga tcaggctccc
     2101 cgaggccgag gactacgcca agaccatcct ccacgacggg gtctacctga ccgcccggga
     2161 caacgacacc ttcctgggcc aggccatggc ggtgttcggc agggcctaca ccaccctcaa
     2221 tttctccctc aggaacgaca gcgaggactc caaggc
//
LOCUS       DASSQV010000115         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2267655, whole genome shotgun sequence.
ACCESSION   DASSQV010000115 DASSQV010000000
VERSION     DASSQV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267655"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="VFL04_02005"
     CDS             complement(<1..1437)
                     /locus_tag="VFL04_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7838504.1"
                     /translation="MRIRRIQTSLIQAFALLIIMTIVLLGAFSLSFFKRTLVGLAEQN
                     TTQLLAQLNRVIENYITYMDDIALVLVNNQDVRAYMAAPQLSPPGSRSRIAQFLGSVR
                     TVRKDIDGIFLLPAEGSAGIIASSPGEAINPNYDFASRYRSLPRSLKARLDAGKPIVS
                     SAHVENIVEGRYPWVISLIRDIGGEGGRAGYIQVDLNYAIIEDLCRNIQLGKSGYVFI
                     LDPSGDIVYHPRQQLVYGNLKSERISAVLGLRGGYLAANVDGRDILYTAVDSAQTGWT
                     VVSVSYMDELFAGSREAEYSFLMLALLCLAVAIMAAAFVSVRISRPIESLRRSMQAVE
                     SGNFDIDITVNSSNEVYQLARDCDIAVKKVRDLIEQNRHDQEQKRDLELRALQAQINP
                     HFLYNTLDSIIWMIELGENERAIDVTSNLARFFRLGINRGSEIVSLRTEMDYLVTYLS
                     IQKIRYKNKLDYEIAFPPELLGLRIPKLL"
     gene            complement(1434..>2196)
                     /locus_tag="VFL04_02010"
     CDS             complement(1434..>2196)
                     /locus_tag="VFL04_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7838505.1"
                     /translation="TAALAYRLVRGPEAPFRYRQTADEAPQSMAAFRAVEERIFLCLR
                     AGAVDQAQLLSSAFLEALAGAGLSPQRVRHEVLALFARALDELGALGISPATVSAEFG
                     ADYYAHAESLGSPAAVGKAVEKLASIAAGFLGTRAMHLPEWKILDFKEYVERHYRDPK
                     LSIARAAEGLSISESYLSKLLRRHLGKSFVDYLTEFRVARARELLAGTDLLTYEVAES
                     VGFADARYFSTVFRRLSGSTPSEYRSAAADRSPAP"
BASE COUNT          354 a          652 c          832 g          358 t
ORIGIN      
        1 gaggagcttg gggatcctga ggccgaggag ctcgggcggg aaggcgatct cgtagtcgag
       61 cttgttcttg tagcggatct tctggatgga gaggtaggtg acgaggtagt ccatctcggt
      121 ccgcagcgag acgatctccg agccccggtt gatcccaagg cggaagaagc gcgcgaggtt
      181 cgaggtcacg tcgatcgccc tctcgttctc gcccagctcg atcatccaga tgatggagtc
      241 cagggtgttg tagaggaagt gggggttgat ctgggcctgg agggcgcgca gctcgaggtc
      301 gcgcttctgc tcctggtcgt ggcggttctg ctcgatgagg tcgcgcacct tcttcacggc
      361 gatgtcgcag tcccgcgcga gctggtagac ctcgttcgag ctgttcacgg tgatgtcgat
      421 gtcgaaattg ccgctctcca ccgcctgcat cgagcggcgc agcgactcga tcggccgcga
      481 gatccgcacc gacacgaagg ccgcggccat gatggcgacc gcgaggcaga ggagggcgag
      541 catgaggaag ctgtactcgg cctcgcgcga gcccgcgaag agctcgtcca tgtacgagac
      601 ggagacgacc gtccagccgg tctgggccga gtccaccgct gtatagagga tatcccggcc
      661 gtcgacgttg gcggcgaggt agccgccgcg gagcccgagg acagccgaga tgcgctcgga
      721 cttgaggttg ccgtagacca gctgctggcg cgggtggtag acgatgtcgc ccgagggatc
      781 gaggatgaag acgtagcccg acttcccgag ctggatgttc ctgcacaggt cctcgatgat
      841 cgcgtagttc aggtccacct ggatgtagcc cgccctcccg ccctcgccgc cgatgtccct
      901 gatgagggag atgacccagg ggtagcggcc ctcgacgatg ttctcgacat gggccgagga
      961 cacgatcggc ttgccagcgt cgagcctggc cttgagcgag cgggggaggg agcggtagcg
     1021 gctggcgaag tcgtagttgg ggttgatcgc ctcgccgggt gaggaggcga tgatcccggc
     1081 cgagccctcg gcgggcagga ggaagatgcc gtcgatgtcc ttcctgacgg tccgcaccga
     1141 gcccaggaac tgggcgatgc ggctcctcga gcctggcggg ctcagctgag gcgccgccat
     1201 gtaggcgcgg acgtcctggt tgttcacgag gacgagggcg atgtcgtcca tgtaggtgat
     1261 gtagttctcg atgacgcggt tgagctgggc gaggagctgg gtcgtgttct gctcggcgag
     1321 gcccacgagg gtccgcttga agaaggacag ggagaaggcc ccgaggagga cgatcgtcat
     1381 tattatgagg agggcgaagg cctggatcag gctggtctgt atcctgcgga tcctcacggc
     1441 gccgggctcc ggtcagccgc ggccgagcgg tactccgagg gggtcgagcc cgagagccgc
     1501 ctgaacacgg tgctgaagta gcgagcgtcg gcgaagccga cgctctcggc cacctcgtag
     1561 gtgaggaggt cggtgcccgc caggagctcc ctcgcgcggg ccacgcggaa ctcggtgagg
     1621 tagtcgacga aggacttgcc gaggtggcgg cgcaggagct tggagagata gctctcgctg
     1681 atcgagaggc cctcggccgc cctcgcgatc gagagcttgg ggtcgcggta gtgccgctcc
     1741 acgtactcct tgaagtcgag gatcttccac tcgggcaggt gcatggccct cgtgcccagg
     1801 aagcccgccg cgatcgaggc cagcttctcc acggccttgc cgaccgccgc tgggctgccg
     1861 agggactcgg cgtgggcgta gtagtcggcg ccgaactcgg cggacaccgt cgccggcgag
     1921 atccccaggg ccccgagctc gtcgagggcg cgggcgaaga gggcgaggac ctcgtgccgc
     1981 acgcgctgcg gggagagccc ggcccccgcc agggcctcga ggaaggccga gctgaggagc
     2041 tgggcctggt ccaccgcccc ggccctgagg cagaggaaga tccgctcctc gacggcgcgg
     2101 aatgctgcca tcgactgcgg ggcctcgtcg gccgtctggc ggtagcggaa gggggcctcg
     2161 gggccgcgca cgaggcggta ggcgagggcg gccgtg
//
LOCUS       DASSQV010000116         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_29147, whole genome shotgun sequence.
ACCESSION   DASSQV010000116 DASSQV010000000
VERSION     DASSQV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29147"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFL04_02015"
     CDS             <1..432
                     /locus_tag="VFL04_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7838506.1"
                     /translation="VAGGPDAGGPNSGAGLKLPPKGIVERESTRVYACPDPLVSKAAT
                     FIRARAHEGIGVPDILEVVSASRRALETRFRAALGRSLHEEILRVRLARAKRLLGETS
                     LTVERIAADCGFGALQRFHAAFKEAEGMSPGEWRRRSRGRD"
     gene            complement(454..1434)
                     /locus_tag="VFL04_02020"
     CDS             complement(454..1434)
                     /locus_tag="VFL04_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HET7838507.1"
                     /translation="MHGGAWNIPAPLRDAHIQGVRKAYRIAHEALASGMAPLRAVVEV
                     LKSLEDDPTFDAGTGSFLNEDGRVELDAAVMEGADLGCGAVAAVGRFRNPSEIALAVK
                     EKTDHVLLIAEGAERFALAQGFETVEPRSLVHPREFEGYEAWVRAGKPDPRRWFDEEG
                     RAGAETGAEGAAQEGSGPPAEGAGAPTEQHKRGTVGVVIGVRKEDESGYRLFSGISTG
                     GTRGKMAGRVGDVPLIGCGIYADDETAAVCCTGWGEAFVRTVAAKSVAERIASGAHPQ
                     EAVEAQLWQLLARTGGRGGIMAVDSRGRMGAGFTTPDMAYAGPGCRRLEL"
     gene            1640..>2300
                     /locus_tag="VFL04_02025"
     CDS             1640..>2300
                     /locus_tag="VFL04_02025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HET7838508.1"
                     /translation="MTRCRYLIVGGGMAGASAIKGIREVDAQGEILAVSAEDVAPYQR
                     PLLSKGLWKGKSFEKVWMKVEAEGLELRFGRRVMGIDAAARTASLDDGPGVSFEKALI
                     ATGSRPRRLPFEDEALLHYRSLADYQRLRALAETGGAFAVVGGGFIGWEIAAALAMNG
                     KKVWLIFPGPSLGHRLFPRDLSLFLNGFYSGKGIELLPGSTLAGLRRVGARLSLGLVG
                     GR"
BASE COUNT          321 a          845 c          805 g          329 t
ORIGIN      
        1 gtggccgggg gccccgatgc cggaggcccc aattcggggg ccggcctcaa actcccgccc
       61 aagggaatcg tggagcggga atccacaagg gtctacgcat gccccgaccc cctggtctcg
      121 aaggcggcca ccttcatcag ggccagggcc cacgagggca tcggggtgcc cgacatcctc
      181 gaggtggtct cggcctcgag gagggccctg gagacaaggt tccgggctgc cctcgggagg
      241 agcctccacg aggagatcct cagggtcagg ctcgccaggg cgaagcgcct cctcggcgag
      301 acttcgctga cggtggagcg gatagcagct gactgcggct tcggcgccct ccagcgtttc
      361 cacgccgcct tcaaggaggc cgagggcatg agcccggggg agtggcgcag gcggagccgc
      421 ggccgggact agggcccgga gccgcggccg ggactagagc tcgaggcggc ggcagcctgg
      481 cccggcgtag gccatgtcgg gggtggtgaa gccggccccc atgcggcccc gcgagtcgac
      541 ggccatgatc cctcccctgc cgccggtgcg cgcgaggagc tgccagagct gggcctcgac
      601 agcctcctgg ggatgggccc ccgaggcgat ccgctcggcg acggacttgg cggccacggt
      661 cctgacaaag gcctcgcccc agcccgtgca gcagacggcg gcggtctcgt cgtcggcgta
      721 gatcccgcag ccgatgaggg gcacgtcccc caccctgccc gccatcttgc ccctggtccc
      781 ccccgtgctg atcccggaga agagccggta gccgctctcg tcctccttcc gcacgccgat
      841 gaccaccccg acggtcccgc gtttgtgctg ctcggtgggc gcccccgccc cttcggcggg
      901 cgggccagag ccctcctgcg ccgcgccctc ggcccctgtc tcggccccgg cccggccctc
      961 ctcgtcgaac catctcctgg ggtcgggctt ccccgccctg acccaggcct cgtagccctc
     1021 gaactcgcgg gggtggacga ggctccgggg ctcgaccgtc tcgaaaccct gggccagggc
     1081 gaagcgctcg gccccctcgg cgatgaggag gacgtggtcg gtcttctcct tgaccgcgag
     1141 ggcgatctcc gaggggttcc tgaagcggcc caccgcggcg acggccccgc agccgaggtc
     1201 ggcgccctcc atcacggcgg cgtcgagctc gacccgcccg tcctcgttga ggaagctccc
     1261 cgtgccggcg tcgaaggtcg ggtcgtcctc gagggacttc aggacctcga cgacggccct
     1321 gaggggggcc atgcccgagg cgagggcctc gtgggcgatg cggtaggcct tcctgacccc
     1381 ctggatgtgg gcgtccctga gcggggcggg gatgttccac gcgcccccgt gcacctggac
     1441 atgccacctc tcgtctccac cctcgaaaat ccaatgtgac ggcacctgga acctcctcgg
     1501 ggcggcctgg ccgcggaaac gaagcatagc acggctttcg ggccggcgcg ccctgcccgc
     1561 ccccctccct caggcggcga ttgcgcggcg cccaagcact gctgataata aacactaacg
     1621 catgggcaag gaggatgcga tgacccgatg cagatacctc atcgtcggcg gcgggatggc
     1681 gggggcctcg gccatcaagg ggatccgcga ggtcgacgcc cagggcgaga tcctcgccgt
     1741 ctcggccgag gacgtggccc cctaccagcg ccccctcctc tccaaggggc tctggaaggg
     1801 caaaagcttc gagaaggtct ggatgaaggt cgaggccgag ggccttgagc tcaggttcgg
     1861 gcgcagggtc atgggcatcg acgcggcggc caggacggcg agcctcgacg acggcccggg
     1921 cgtcagcttc gagaaggccc tcatcgccac gggatcgagg ccacggcggc ttcccttcga
     1981 ggacgaggcc ctcctccact accggagcct cgccgactac cagaggctca gggccctcgc
     2041 cgagaccggc ggggccttcg ccgtcgtggg cgggggcttc atcggctggg agatcgccgc
     2101 cgccctcgcg atgaacggca agaaggtctg gctcatcttc cccggcccct ctctcggcca
     2161 caggctcttc ccccgcgacc tctcgctctt cctcaacggc ttttactcgg ggaagggcat
     2221 cgagctcctc cccggctcaa ccctggcggg gctcaggcgc gtcggcgccc ggctctcgct
     2281 cggcctcgtg gggggcagga
//
LOCUS       DASSQV010000117         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2268248, whole genome shotgun sequence.
ACCESSION   DASSQV010000117 DASSQV010000000
VERSION     DASSQV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2268248"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VFL04_02030"
     CDS             <1..1307
                     /locus_tag="VFL04_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HET7838509.1"
                     /translation="VQEELLQELEDKPRYREPILVAEGTKPQNGRDSRIQFNFETDKT
                     KYRLKEASDGKVNFKELGLIQNVVAGQPLARKVPPETGIQGRTVTGKIMPARNGRDVI
                     APLGRNVHMAEDGATIIADINGQVTFLAGRINVEEIFTVPGDVNLKTGNIMFLGTVII
                     QGNVDDGFTVKASGNIEITGSVGKSEIVAEGDVVVHQGVAGKSGGSVTAGKNIWAKFI
                     ENAEVEAGENVIVSDGLINSNIVANKKIICQGKRAAIVGGSYRACEEINAKSLGSPVG
                     GAETILEVGYDPKSKEKMDELQNQALKVKRQVDEHDKNIATLKAIKKQKKTLPEDKEA
                     ILQDYTVRRSELIAELRALAKEIEAIQTYLNGLKTRGRISASGRVYPGVKLVIKDIHE
                     EVKNEQKSITFYLENQMIKTTKYEEPEDEILKRGPPDAHKAD"
     gene            1288..1611
                     /locus_tag="VFL04_02035"
     CDS             1288..1611
                     /locus_tag="VFL04_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838510.1"
                     /translation="MPIRPIDLQTLLMQLSQVGRDQAAEKDAALRASMQTAADQVKQV
                     EQKEAVRHAQEPDTGPQAIKDRQGGGPGGSSLKKKEEGEPEAAEGEDEVIRDPSLGNS
                     IDISG"
     gene            1617..>2067
                     /locus_tag="VFL04_02040"
     CDS             1617..>2067
                     /locus_tag="VFL04_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838511.1"
                     /translation="MAQFLTILALDIAGLFTVWFLLRARIRRFLELENLLADVRAESR
                     ALVMELNETADRNVSLVEDRISSLRELLEEVDRRIGVAHRELDARRAERAVYDQLSRR
                     RPIVPQTGGTPGSEAPRRPEPAPSGAQAEYRSAARPEPRAEARAEAGA"
BASE COUNT          454 a          676 c          692 g          245 t
ORIGIN      
        1 gggtccagga ggagctcctc caggagctcg aggacaagcc ccgctaccgc gagcccatcc
       61 tcgtcgccga gggcaccaag ccccagaacg gcagggactc gcggatccag ttcaatttcg
      121 agaccgacaa gaccaagtac cgcctcaagg aggcctcgga cggcaaggtg aacttcaagg
      181 agctgggcct catccagaac gtggtcgcgg gccagcccct ggcaaggaag gtgcctcccg
      241 agacggggat ccagggaagg acggtcaccg gcaagatcat gccagcccgc aacggcaggg
      301 acgtgatagc ccccctgggc aggaacgtcc acatggccga ggacggggcc acgatcatcg
      361 ccgacatcaa cggccaggtc accttcctcg cgggcaggat caacgtcgag gagatcttca
      421 ccgtccccgg cgacgtgaac ctcaagaccg gcaacatcat gttcctcggg acggtgatca
      481 tccaggggaa cgtcgacgac ggcttcaccg tcaaggcctc gggcaacatc gagatcacgg
      541 gctcagtcgg caagtcggag atcgtggccg agggcgacgt cgtggtccac cagggggtcg
      601 cgggcaagtc ggggggctcg gtgaccgccg gcaagaacat ctgggccaag ttcatcgaga
      661 acgccgaggt cgaggcgggc gagaacgtca tcgtctccga cggcctcatc aactccaaca
      721 tcgtcgccaa caagaagatc atctgccagg gcaagcgcgc ggccatcgtc ggcgggagct
      781 acagggcctg cgaggagatc aacgcgaaga gcctcggctc gccggtcggc ggggccgaga
      841 cgatcctcga ggtgggctac gaccccaaga gcaaggagaa gatggacgag ctccagaacc
      901 aggccctcaa ggtcaagcgc caggtcgacg agcacgacaa gaacatagcc accctcaagg
      961 cgatcaagaa gcagaagaag accctccccg aggacaagga ggccatcctc caggactaca
     1021 ccgtgcggcg ctccgagctc atagccgagc tgcgtgccct ggccaaggag atcgaggcca
     1081 tccagaccta tctcaacggc ctcaagacga gggggaggat cagcgcctcg ggcagggtct
     1141 atcccggcgt caagctcgtg ataaaggaca tccacgagga ggtcaagaac gagcagaagt
     1201 ccattacctt ctacctcgag aaccagatga tcaagaccac gaagtacgaa gagcccgagg
     1261 acgagatact caaacggggg ccacccgatg cccataaggc cgattgacct gcagaccctc
     1321 ctcatgcagc tcagccaggt cggacgcgac caggctgccg aaaaggacgc cgccctccgc
     1381 gcctccatgc agaccgccgc cgaccaggtg aagcaggtcg agcagaagga ggccgtccgc
     1441 cacgcccagg agcccgacac cgggccccag gcgatcaagg accgccaagg gggcggcccg
     1501 ggcggctcct cgctgaagaa gaaggaggag ggggagcccg aggcggccga aggcgaggat
     1561 gaggtcatca gggaccccag cctcgggaat tcgatcgaca tatcgggatg acggccatgg
     1621 cgcagttcct cacgatcctg gccctcgaca tcgcgggcct ttttaccgtc tggttcctcc
     1681 tgcgggcgcg catcaggcgt ttcctcgagc tcgagaacct cctcgccgac gtcagggccg
     1741 agtcgagggc cctcgtcatg gagctgaacg agacggccga ccgcaacgtg agcctcgtcg
     1801 aggacaggat ctcctccctc cgcgagctcc tcgaggaggt cgaccggcgg atcggcgtcg
     1861 cccaccgcga gctcgacgcg cgcagggccg agagggctgt ctacgatcag ctgagccgcc
     1921 gcaggcccat cgtgccccag acgggcggga cgcccggcag cgaggccccg aggcggcccg
     1981 agcctgcccc cagcggagcc caggcggaat atcggagcgc ggcgcggccc gagccaaggg
     2041 cggaggcgag ggcggaggcg ggggcgg
//
LOCUS       DASSQV010000118         6268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2143927, whole genome shotgun sequence.
ACCESSION   DASSQV010000118 DASSQV010000000
VERSION     DASSQV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143927"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1521)
                     /locus_tag="VFL04_02045"
     CDS             complement(7..1521)
                     /locus_tag="VFL04_02045"
                     /inference="COORDINATES: protein motif:HMM:NF033751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pallilysin-related adhesin"
                     /protein_id="HET7838512.1"
                     /translation="MKKLVQAIFLLFCAIALGIAVYYLVRERSGAAAAAKPVTTRQVV
                     VSNDGAGAEERDPRAGRAAAVNHIAALPNEVILDVAPINLIQNEGGEGEEQVLTVRKT
                     DRPDGRLFVVVAEYLPTRKTWVRAWEGETLATKLTTFTIQAKDLIGDHILNIVCMGMN
                     EEDEQTVTVFRRNPGTSLLGYTMIFSTAADAIAIGETERSEGYQLGQTNGDSWPVYAF
                     RHDKDSANLLDQVKTSYAWDYKRGAFLKAGEERIPGAQVEHDMVAKVLTGSEKDFESF
                     LQGLWYESGKGPLDPGTRMLVFDRQGGSITFYSTESQEVFRWTDSHSTRYGLYVGSQN
                     ESVSNLRRLMDLELTGADSISVRVFEDLQMKVDAENRWDGSYRKLPPNLSESLSRGAK
                     PAPAMKLAGRFISADGTELEFASPRYELRKGQATERGAFTVFGLGDDTVMEFRVQEGG
                     ASGSSRRTYGASFSETKSGKQILRRLVLSPARTSLMGLELLQEPSLVLEQRVNG"
     gene            complement(1518..2498)
                     /locus_tag="VFL04_02050"
     CDS             complement(1518..2498)
                     /locus_tag="VFL04_02050"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET7838513.1"
                     /translation="MTLDDFADQLKSHDKYLVLSHDGPDADGLGSAYALVIALRSIGK
                     RAMAAVSDQLPAKFRFIDRQGLFVSLATEAGFGFPPEEATVIVVDTHDLHYLGARGAE
                     LLKRCARHMVIDHHEARSAPGPLLLLESEASSTCELVYLLARSLGAEVPEDAAEALFA
                     GMVYDTGSFAYAKTGERTFACARELVALGAKPYKIHREMYESSSVAVLILQKMVFASL
                     ELFAEDRIAVQTLRRTDLSASGANYEDAEDLVNRPLIGGTIEVSILFKENLEGRLRCS
                     LRSKGRVNVARIAQSYGGGGHKTAAGFTCPEPLERIKDDVLQSIGKALTA"
     gene            complement(2485..3441)
                     /locus_tag="VFL04_02055"
     CDS             complement(2485..3441)
                     /locus_tag="VFL04_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET7838514.1"
                     /translation="MNGRFRLPPRLPPLLLLAVLALSPSFPQAAGPGTKVDPRSGDLD
                     MVLLLDKSLSMAPFFAEAKSYVAGDVLGPILMPGDRLVIELVYGKVERLWSGTIESEA
                     DKAAAIRRIRAVVADGAYTDLGAGLDAAKRDVDELGRPERPKYVLLVTDERQEAPPGS
                     AYPAADYRLKHPYLEYVKKRDLGRFRAITVGIGVGAKVEAAASQVMKYLAEPPARGGF
                     GGTSLESSEAAAAAAKAQAAGGGGASGSPSPAQGPGQAQGQAQGQTPGAAAQAGGPGR
                     GALPPWALPGGAVVLALAIGLLALLQLKKGKKSPQKDAHDAG"
     gene            3638..5698
                     /gene="cfpA"
                     /locus_tag="VFL04_02060"
     CDS             3638..5698
                     /gene="cfpA"
                     /locus_tag="VFL04_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014342563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic filament protein CfpA"
                     /protein_id="HET7838515.1"
                     /translation="MGAIDLPKSPNVFHPEKPSAVGSRNSLAQEGRDQQREVNQLLEE
                     ETNKVIHHMNARLPKEVLERLDVMGGLKEKLYNYFNQNYQNMFNRFMVTTEDEMVKKV
                     RNFIDKEEMKSLARYTPKEIAELLDQVGGADKFNTGEVEKSMVNMFGHLQGHIQRGVN
                     ELENLTNALLRQKTDVGAFIRGENAYSVVKCSFKDDNYKPKTVTNVKLSVNILDSELI
                     SPIFHYQVTVEYLIKDLISKHLIDQIDKEIEKLKDRLVDAGKEELSDTEIIFEKMNRI
                     RDYTDDEADNPKSKRYSIIGKELMERISNLRAEIDPATFDQLNIRENLKKIVDIENIR
                     NRGYNTAVNSITSILDTSKMGYQYIENLKNARELIIREYEDTNFALLPDERYQIRLQY
                     YDNAQLIEERKAYDVQMKSFETEVQHLGDVLEMMYEDSKFLKRITDFNDLAGIYKRKI
                     RTRIKSKTQEPVYEDISKVWDEISFVRPAETEVEKMNRSYLYEKDKVRNKIILMAEKL
                     KNMYSYQYPIQRRVVEERLAFLSKEFDKFAYIINPYHIQPGLLLDVDITSIKRKKATL
                     DGMANVLNEFLHGVSKGFQDAAFAAFSRRRSTVREDIAQSFSPGGEAEAQGAPGADYL
                     SMLNTEPSPALEASIEPPVNVKKAPAKRGPKAKGVVDTGKKGAAKPAGRRGRRGSEGG
                     LREV"
     gene            5852..>6268
                     /locus_tag="VFL04_02065"
     CDS             5852..>6268
                     /locus_tag="VFL04_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838516.1"
                     /translation="MKSLNSQFEGLADRESFNGTVRHIKTVISVVERVAAKENLADVV
                     AELVEAKTLDKDEVRPVAAMVLGDKYGYRSVAANLESVANDLSEVSAEIAGWNGVDLV
                     LLYSHPDLGVIAVNPKNKRQVAQIDRLNRFELAVVYA"
BASE COUNT         1163 a         2029 c         2018 g         1058 t
ORIGIN      
        1 ccggctctag ccgttgaccc gctgctcaag gaccaggctc ggctcctgaa gtagctcgag
       61 acccatgagc gaggtgcggg ccggcgagag cacgagcctt cgcaggatct gttttccgct
      121 tttggtctcg ctgaagctcg ccccataggt ccgacgcgag gagcccgaag cgcctccctc
      181 ctggaccctg aactccatga cggtgtcgtc gcccaggcca aaaacggtga aggccccgcg
      241 ttcggtcgcc tgtcccttcc tgagctcgta gcggggcgag gcgaactcga gctccgtccc
      301 gtcggccgag atgaagcgcc cggccagctt catggccggc gcgggtttcg ccccccgcga
      361 gaggctttcg ctcaggttcg gcggaagctt ccggtagctg ccgtcccagc ggttctcggc
      421 gtcgaccttc atctggaggt cctcgaagac cctgaccgag atcgagtcgg ccccggtaag
      481 ctcgaggtcc atgaggcggc ggaggttgga caccgactcg ttctggcttc ccacatagag
      541 cccgtagcgg gtcgagtggg agtcggtcca gcggaagacc tcctgggact cggtcgagta
      601 gaaggttatg gatccgccct gcctgtcgaa gacgagcatc ctggtccccg ggtcgagggg
      661 gcccttgccc gactcgtacc agaggccctg gaggaagctc tcgaaatcct tctccgagcc
      721 cgtgagcacc ttggcgacca tgtcgtgctc gacctgggcc ccagggatcc tctcctcgcc
      781 ggccttgagg aaggccccgc gtttgtagtc ccaggcgtag ctcgtcttca cctggtcgag
      841 gaggttcgca gaatccttgt cgtggcggaa ggcgtagacc ggccagctgt cgccgttggt
      901 ctgcccgagc tggtagccct cggacctctc ggtctcgccg atggcgatgg cgtcggccgc
      961 cgtggagaag atcatggtgt agccgaggag ggaggtgccg ggattccttc ggaagacggt
     1021 cacggtctgc tcgtcctcct cgttcatgcc catgcagact atgttgagga tgtggtcgcc
     1081 gatgaggtct ttggcctgga tggtgaaggt cgtgagcttg gtggcgaggg tctcgccctc
     1141 ccaggccctg acccaggtct tcctggtggg gaggtattcg gcgacgacga cgaagagccg
     1201 tccgtcgggg cggtcggtct tcctgacggt caggacctgc tcctcgccct cgcccccctc
     1261 gttctggatg aggttgatgg gcgcgacatc gaggatcacc tcgttgggga gggcggcgat
     1321 gtggttgacg gcggctgcgc gcccagcccg ggggtcgcgc tcttccgccc ctgccccgtc
     1381 gttcgacaca accacctgcc tcgtcgtgac cggcttggct gcagccgcag cgcccgagcg
     1441 ctccctgacc aggtagtaca cggcgatgcc cagcgcgatc gcgcagaaga ggaggaatat
     1501 ggcctggacg agtttcttca tgcggtgagt gccttgccga tgctttgtag cacgtcgtct
     1561 ttgatgcgtt caaggggctc ggggcaggtg aagcctgccg ccgtcttgtg cccgccaccg
     1621 ccataggact gggcgatccg cgccacgttg acccggccct tcgagcgaag ggagcagcgc
     1681 agcctcccct cgaggttctc tttgaagagg atcgagacct cgatcgtgcc gcctatcagg
     1741 ggcctgttga cgaggtcctc ggcgtcctcg tagttggcgc cggaggcgct gaggtcggtc
     1801 ctgcggaggg tctggactgc tatcctgtcc tcggcgaaga gctcgaggct cgcgaacacc
     1861 atcttctgca ggatgagcac ggccacggag gaggattcgt acatctccct gtggatcttg
     1921 tagggcttcg cgccgagggc cacgagctcg cgggcgcagg cgaaggtgcg ctcgcccgtc
     1981 ttcgcgtagg cgaaggagcc tgtgtcgtac accatgccgg cgaaaagggc ctcggccgcg
     2041 tcctcgggga cctccgcgcc gaggctccgg gcgaggaggt acacgagctc gcaggtgctc
     2101 gaggcctcgc tctcgaggag gagaaggggg ccaggcgcgg atcgggcctc gtggtggtcg
     2161 atgaccatgt gccgggcgca gcgcttgagc agctccgccc cgcgcgcccc aagatagtgg
     2221 aggtcatggg tgtccaccac gatgaccgtc gcctcctcgg gggggaagcc aaacccggcc
     2281 tccgtcgcga gcgagacgaa aagcccttgg cggtctatga acctgaactt ggcggggagc
     2341 tggtcggaga cggccgccat ggcccgcttg ccgatgctcc ggagcgcgat gacgagggca
     2401 taggccgagc ccaggccgtc ggcgtcgggc ccgtcgtggg acaggaccag gtatttgtca
     2461 tggctcttca gttgatcggc gaagtcatcc agcgtcatgc gcgtcctttt ggggagactt
     2521 tttccctttt ttcaattgta atagggccaa aaggccgatc gcaagcgcga gtaccacagc
     2581 cccaccgggc agggcccagg gggggagggc gcccctgccc gggccgccgg cctgggctgc
     2641 cgcgccggga gtctggcctt gggcctggcc ttgggcctgg cccgggccct gggccgggga
     2701 cgggctgccc gaggccccgc ccccgccagc ggcttgggcc ttcgccgcgg ctgccgcggc
     2761 ctcgctggat tccaggctcg tgcccccgaa gccgcctcgg gccgggggct cggcgagata
     2821 tttcatcacc tgcgaggcgg cggcctcgac cttggcgccg accccgatgc ccacggtgat
     2881 ggccctgaac ctgccgaggt cgcgcttttt cacgtactcg aggtaggggt gcttgagcct
     2941 gtagtcggcc gcgggatagg cggaaccggg tggcgcctcc tggcgctcgt cggtcacgag
     3001 gaggacgtat ttcgggcgct cggggcggcc aagctcgtcg acgtcgcgct tcgccgcgtc
     3061 caggcctgcg cccaggtccg tgtaggcccc gtcggcgacc acggcccgga tcctccgtat
     3121 ggcggcggcc ttgtcagcct cggactctat ggtgcccgac cagagtcgct caacctttcc
     3181 gtagacgagc tctatgacga gcctgtcgcc gggcatgagg atggggccga gcacgtcgcc
     3241 cgcgacatag ctcttggcct cggcgaagaa gggcgccatg gagagggatt tgtccaggag
     3301 gaggaccatg tcgaggtccc cgctgcgcgg atccaccttg gtgccggggc cggcggcctg
     3361 ggggaacgaa ggcgagagcg cgaggacggc caggaggagg aggggtggga gccggggggg
     3421 gaggcggaat cgaccgttca tgagcctgcc tgcctttccg cggaaccgcg cgttttggcc
     3481 aaaaaacggt caggatggcc gcgcggcgcc gtcattcggt gatattctac catattaggg
     3541 agtcgcaagg cctcgaaaat tccttttcgg cctttacaac tcaattcgcc attcgatact
     3601 ataacccaag aatcgctatt cgcctaaagg agatcgtatg ggtgcgatag atctgcctaa
     3661 gagtccaaac gtattccacc ccgagaagcc ctcggccgtc ggatcccgca actccctcgc
     3721 tcaggagggc cgcgaccagc agcgcgaggt gaaccagctc ctcgaggaag agaccaacaa
     3781 ggtcatccac catatgaacg cccgcctccc caaggaggtc ctggagcgtc tggacgtgat
     3841 gggcgggttg aaggagaagc tctacaacta cttcaaccag aactaccaga acatgttcaa
     3901 ccgcttcatg gtcaccaccg aggacgagat ggtgaagaag gtgaggaact tcatcgacaa
     3961 ggaagagatg aagtccctcg cccgctatac cccgaaggag atcgccgagc tcctcgacca
     4021 ggtcggcggc gctgacaagt tcaacacggg cgaagtcgag aagtcgatgg tcaacatgtt
     4081 cggccacctc cagggccaca tccagcgcgg cgtgaacgag ctggagaacc tgaccaacgc
     4141 cctcctccgc cagaagacgg acgtcggcgc cttcatccgc ggcgagaacg cctactcggt
     4201 cgtcaagtgc tcgttcaagg atgacaacta caagcccaag accgtcacca acgtcaagct
     4261 ttccgtcaac atcctcgact ccgagctcat ctccccgatc ttccactacc aggtcacggt
     4321 ggagtacctc atcaaggacc tgatctccaa gcacctcatc gaccagatcg acaaggagat
     4381 cgagaagctc aaggaccggc tcgtggacgc gggcaaagag gaactctcgg ataccgagat
     4441 catcttcgag aagatgaacc gcatccgcga ctacacggac gacgaggcgg acaatcccaa
     4501 gtccaagcgc tacagcatca tcggcaagga gctcatggag aggatctcca acctccgcgc
     4561 cgagatcgac cccgccacct tcgatcagct caacatccgc gagaacttga agaagatcgt
     4621 cgacatcgag aacatccgca accgcggcta taacaccgcc gtcaactcga tcacctcgat
     4681 cctcgacacc tcgaagatgg gctaccagta catcgagaac ctcaagaacg cccgcgagct
     4741 catcatcagg gagtacgagg acacgaactt cgccctcctc cccgacgagc gctaccagat
     4801 caggctccag tactacgaca acgcccagct catcgaggag cgcaaggcct acgacgtcca
     4861 gatgaagagc ttcgagaccg aggtccagca cctcggcgac gtgctcgaga tgatgtacga
     4921 ggactccaag ttcctgaagc gcatcaccga cttcaacgac ctggccggga tctacaagcg
     4981 caagatccgc acccgcatca agtccaagac ccaggagccg gtgtacgagg acatctccaa
     5041 ggtctgggac gagatctcct tcgtcaggcc ggccgagacc gaggtcgaga agatgaaccg
     5101 cagctacctc tacgagaagg acaaggtgcg gaacaagatc atcctcatgg ccgagaagct
     5161 caagaacatg tactcctacc agtacccgat ccagcgccgg gtcgtggagg agcgccttgc
     5221 cttcctgtcc aaggaattcg acaagttcgc ctacatcatc aacccctacc acatccagcc
     5281 cggcctcctc ctcgacgtgg acatcacctc catcaagagg aagaaggcga ccctggacgg
     5341 catggccaac gtgctcaacg agttcctcca cggggtgtcc aagggcttcc aggacgcggc
     5401 cttcgcggcc ttcagccgcc gccgctccac tgtgcgcgag gacatcgccc agtccttctc
     5461 ccccggcggt gaggccgagg cccagggggc tcccggagcc gactacctgt ccatgctgaa
     5521 caccgagccg agccccgccc tcgaggcgtc catcgagccc ccggtcaacg tcaagaaggc
     5581 tccggccaag cgcggaccca aggccaaggg cgtcgtcgac acgggcaaga agggcgcggc
     5641 caagccggct ggtcggcgag gcagacgcgg ttccgaggga ggcctcaggg aggtctgaac
     5701 ccgtgcgggt ttcgcgccct gaagcggcgg cgccgcccca tcgccatggc cttgtgtcac
     5761 gcgatgggcg cagtatcatc tagggtaagc ccgaggggcc gttcctccgc acaccggggg
     5821 aacggcctcc ttattaagag gagtccggcc catgaagagc ctcaatagcc agttcgaagg
     5881 actcgccgac cgtgagagct tcaacggcac cgtccggcac atcaagacag tcatttcggt
     5941 cgtcgagcgc gtcgccgcga aggagaacct cgcggatgtc gtagcggagc tcgtcgaggc
     6001 gaagaccctc gacaaggatg aggtccgccc agtcgcggcc atggtgctcg gggacaagta
     6061 cggctaccgg agcgtggcgg cgaacctcga gtcggtcgcg aacgacctgt ccgaggtgtc
     6121 ggccgagatc gccggctgga acggcgtcga cctcgtcctc ctgtactccc atcccgacct
     6181 cggcgtcatc gccgtcaacc cgaagaacaa gcggcaggtc gcccagatag accgcctgaa
     6241 ccgcttcgag ctcgccgtcg tctacgcg
//
LOCUS       DASSQV010000119         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1148441, whole genome shotgun sequence.
ACCESSION   DASSQV010000119 DASSQV010000000
VERSION     DASSQV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148441"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1059)
                     /locus_tag="VFL04_02070"
     CDS             complement(49..1059)
                     /locus_tag="VFL04_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET7838517.1"
                     /translation="MASSFQASYVYARLCGSFARSCVGERAAALARLGRVGELWKGIF
                     NEEPPPLSEPALVAAAELRTMARAREAFTRIAGPTAAEEPVFVALLRKDEFAQVKRIV
                     LAVKEGGPRPSEPELRSWSPDYDISAYPELEGIFARGRFSWIPELGLDDPTLLRNRLD
                     RQYYAELWDSLNAVGEELLGSIRGLVRLEAELQNLVWALRLRRYYAYKAEQIEGLLIR
                     LGGLDVAGTARAALGFRGEVRGDWNGWRYEALVNGPAKPGEDWYLDIPRFEAELRLHL
                     YRRLKRGLHAEPFSYAPLYCYYRLREYETASILGLIEGAQLGMAAEELAGFALGTGGG
                     RE"
     gene            complement(1059..1331)
                     /locus_tag="VFL04_02075"
     CDS             complement(1059..1331)
                     /locus_tag="VFL04_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838518.1"
                     /translation="MEHRAEELVQEARAEAERRISAAKDAIERDRAARIEAALAGAKR
                     AREAAEDSSEAEYRKALESYKRLLEAAPVDEAAFREACERGLSGAI"
     gene            complement(1402..1872)
                     /locus_tag="VFL04_02080"
     CDS             complement(1402..1872)
                     /locus_tag="VFL04_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7838519.1"
                     /translation="MQLQQIFTARCIKVGLESEEKDELFEELVELLDRDAGKAFPRGA
                     ALSAIRDRESKMSTGIKKGIALPHGKVSGISGLFGAIGISRRGIDYDALDGEPVYLVF
                     LLISSPEDSELHLSALKRLAGLLDDPEFYTQLLRADTPERAFAILKSYEDVLHL"
     gene            complement(1912..2304)
                     /gene="secG"
                     /locus_tag="VFL04_02085"
     CDS             complement(1912..2304)
                     /gene="secG"
                     /locus_tag="VFL04_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET7838520.1"
                     /translation="MGILSIVLLVIFVIVALLLVLMVIIQDSDSDSLGGLFAGGSSSA
                     FGSRSTSVVVRFTYVLGALFFVVAFGLAILNKSSVGNVEAAAQQKNQSTPTEWWNVQP
                     KSANPGLPGAEQVPPAQTPATPVPTPGK"
     gene            complement(2511..3263)
                     /gene="tpiA"
                     /locus_tag="VFL04_02090"
     CDS             complement(2511..3263)
                     /gene="tpiA"
                     /locus_tag="VFL04_02090"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET7838521.1"
                     /translation="MKQYYIAGNWKMHKTVGESVAFARELVGKLKGSKEKLMIAPAFT
                     ALAGVAEALRGSSIILGAQNMGPEEQGAHTGEVSVLMLKDLGVRAVILGHSERRHSYL
                     ETDALVNAKLLLAIKHGLDPILCVGETLEEREGGRLESVVGTQLKAGLAGLPASDLAK
                     VTIAYEPVWAIGTGKTATPEDADAVHAYIRKVLSGLYGEAAARAMCIQYGGSVKADNV
                     AALMAKPNIDGALVGGASLKAESFGPIALFRA"
BASE COUNT          535 a         1116 c         1174 g          600 t
ORIGIN      
        1 tgtcccgttt gaggacgaag agctcgacct tccgcatctg gacggggctc actcgcggcc
       61 tccgccggtc ccgagggcga aaccggccag ctcttcggcg gccatcccga gctgtgcgcc
      121 ctcgatcagg ccgaggatgg aggcggtctc gtactcgcgc agccggtagt agcagtacag
      181 cggtgcatag ctgaagggct cggcgtggag tccgcgcttg agcctccgat agaggtggag
      241 cctgagctcg gcctcgaaac gcgggatgtc gaggtaccag tcctcgcccg gcttggcggg
      301 cccgttgacg agggcctcgt acctccagcc gttccagtcc cccctgacct cgccgcggaa
      361 gcccagggcg gccctcgccg tccccgcgac gtcgagcccc ccgagcctga tgaggaggcc
      421 ctcgatctgt tcggccttgt acgcgtagta acgccggagc ctgagggccc agaccaggtt
      481 ctggagctcg gcctcgagcc tcaccaggcc gcgtatggat ccgaggagct cctcgccgac
      541 ggcattgagg ctgtcccata gctcggcata gtactgccgg tcgaggcggt ttctgagcag
      601 ggtcggatcg tcgaggccga gctcagggat ccatgagaag cggcccctgg cgaagatccc
      661 ctccagctcg ggataggccg agatgtcgta gtcgggggac caggacctca gctcgggctc
      721 gctcggtcgg gggccgccct ccttgacggc gaggacgatg cgcttgacct gggcgaactc
      781 gtccttgcga aggagggcca caaatacggg ttcctcggcc gctgtggggc cggcgatgcg
      841 cgtgaaggcc tcgcgggctc gcgccatcgt cctgagctcg gcggcggcca ccagggcggg
      901 ctcggacagg ggcgggggct cctcattgaa gatccctttc cagagctcgc cgacccgccc
      961 cagcctcgcg agcgccgctg ccctctcccc gacgcaggag cgggcgaagg agccgcagag
     1021 ccgggcgtag acataggaag cctggaagct cgaggccatc agatggctcc ggagagaccg
     1081 cgctcgcagg cttccctgaa tgccgcctcg tcgaccgggg cggcctcgag gagccttttg
     1141 taggactcga gggccttgcg gtactcggcc tcggacgagt cctcggccgc ctccctggcc
     1201 cgcttcgccc cggctagggc cgcctcgatt cgcgcggccc tgtccctctc gatggcgtcc
     1261 ttggcggccg agatccggcg ctcggcctcg gcgcgcgcct cttggacgag ttcctctgcc
     1321 ctgtgctcca cctcaaaaag cttctccaga acgtcttgag aaggcatacg cgctcctctt
     1381 ccgtcatgat cgagccttga gctagaggtg caggacgtcc tcgtagctct tgagaatggc
     1441 gaaagcccgc tccggcgtgt cagcgcggag gagctgggtg tagaactcgg gatcgtcgag
     1501 gaggccggcg aggcgcttca gggcgctcag gtgaagctcg gaatcctcgg gggaggagat
     1561 gaggaggaag acgaggtaga cgggctcacc gtcgagggca tcgtagtcga tcccccgacg
     1621 ggatatgccg atggcgccga agaggcccga gatgccgctg accttgccgt ggggaagggc
     1681 tattcccttc ttgatgcccg tcgacatctt ggactctcgg tcgcggatcg cgctcagcgc
     1741 cgccccgcga gggaaggcct ttccggcgtc cctgtcgagg agctccacca gctcctcgaa
     1801 gagctcgtcc ttctcctcgc tctcaagacc gacctttatg caacgggcgg tgaagatctg
     1861 ttggagctgc atgaagcctc cccgacccgc ttcccgaggc cggaaacttc cttacttgcc
     1921 cggggtcgga accggggtcg ccggcgtctg ggccggaggg acctgctccg cgcccgggag
     1981 tccgggattc gcgctcttgg gctgcacgtt ccaccactcc gtcggcgtcg actggttctt
     2041 ctgctgggcc gccgcctcga cattgcccac ggagctcttg ttcaggatcg ccaggccgaa
     2101 ggccacgacg aagaacaggg cgccgaggac atatgtgaac cggacgacca cgctcgtgga
     2161 gcgggatccg aaggcgctcg agctgccgcc ggcgaagagg cccccgagcg agtcggagtc
     2221 cgagtcctgg atgatgacca tgaggacgag gaggagggcg acgatcacga agatgacgag
     2281 gaggactatg ctgagaattc ccatgcttta aactccgggc tgcgcagtgc cggtatgtcc
     2341 cccaagggga gctttccgga tcgtggccgc tatcgtatcg agaaaaacgg ggcaatgcaa
     2401 gacctgtttc cccctcgccg gcgatgcgcg acacggggcc cccgccaagg gcgccgccga
     2461 gggacgggcg gggcccggcc cttggcggcg cgggccccgc ggaagccagg ctaggcccgg
     2521 aacagggcta tcgggccgaa gctctccgcc ttcaggctcg cgccgccgac cagggctccg
     2581 tcgatgttgg gcttggccat gagggcggcg acgttgtcgg ccttgaccga acccccgtac
     2641 tggatgcaca tggccctggc ggccgcctcg ccgtagaggc ccgacaggac cttccgtata
     2701 tacgcgtgga cggcgtcggc gtcctcgggc gtggctgtct ttccggtgcc gatggcccag
     2761 accggctcgt aggcgatggt gaccttggcc aggtcggagg cggggaggcc agcgagcccc
     2821 gccttgagct gggtgccgac gacggactcg agccggccgc cctcgcgctc ctcaagggtc
     2881 tcgcccacgc agaggatggg gtcgaggccg tgcttgatcg ccaggaggag cttggcgttg
     2941 accagggcgt cggtctcgag atagctgtgc cggcgctctg agtggccgag gatgacggcc
     3001 ctgacgccga ggtccttgag catgaggacc gagacctcgc ccgtgtgcgc tccctgctcc
     3061 tcggggccca tgttctgggc gcccaggatg atggaggagc ccctcaatgc ctcggccacg
     3121 ccagcgaggg ccgtgaaggc gggggcgatc atgagctttt ccttgctgcc cttgagcttg
     3181 ccgacgagct cccgggcgaa ggccaccgat tcgcccacgg tcttgtgcat cttccagttg
     3241 ccggcgatgt agtactgttt cacggtaaga ctcctttagt ctgggaaacc gcagaggcgc
     3301 agagggaatg ttggtgagcg cagaagattt ggcaggaggg atgatcccat tcctttttct
     3361 ccaattcccc tgtttctccc catccctctt aactctgtgt ctctgcggtt ctcttattat
     3421 ctcag
//
LOCUS       DASSQV010000120         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1522377, whole genome shotgun sequence.
ACCESSION   DASSQV010000120 DASSQV010000000
VERSION     DASSQV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522377"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VFL04_02095"
     CDS             complement(<1..167)
                     /locus_tag="VFL04_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET7838522.1"
                     /translation="MGKWWEEAVFYQIYPRSFADSNGDGIGDLPGITGKLDYLARLGV
                     NALWISPFFTSP"
     gene            complement(219..551)
                     /locus_tag="VFL04_02100"
     CDS             complement(219..551)
                     /locus_tag="VFL04_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7838523.1"
                     /translation="MGRIRIPRIGVEVETSPAFSILNSLLSAGVEIRHDCGGKALCGT
                     CRLRVLSGASGLSPIGPREAERLDAIRASGAIPPSSGTGDGTIRLACQAHASREAEVE
                     IMLAKGGG"
     gene            complement(559..1602)
                     /gene="dgcA"
                     /locus_tag="VFL04_02105"
     CDS             complement(559..1602)
                     /gene="dgcA"
                     /locus_tag="VFL04_02105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcA"
                     /protein_id="HET7838524.1"
                     /translation="MPETSDLDLVREEYEKQIYDLRQLLEVSKSLNSTLDYHILIDSI
                     LLTIMGQMKVLKAGLFAKKGLDATYFSLHRNYKGFDIDHSVNYSIPEGNELIVLFTHS
                     NRCFTMAEAMARIGSTKGIEGLASLKPTLLVPLRAKGAVNGLIVLGDRIDDSDFEEGE
                     KEYVLNIATLAAIAIHNVFLFEMTTTDMMTRLKMKHYFYTVLIEKMEHSSMSGRPLSV
                     IMIDIDFFKKFNDVYGHNCGDAVLKQVAKLLQACIRGMDLAARYGGEEFCVLLPDTDR
                     ERALIIAERIRKTIAETPSVYEGERLWVTVSLGLAQYIPGRDISGKSLIDRADRALYQ
                     SKQSGRDRICAAE"
     gene            complement(1728..2495)
                     /locus_tag="VFL04_02110"
     CDS             complement(1728..2495)
                     /locus_tag="VFL04_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HET7838525.1"
                     /translation="MDVKAIVIAGFGFNADAELARAFALAGAEVERVHLSDILAEPER
                     LRSARILAFPGGFSFGDHLGSGQVVSMLCRRRLRPALLRFVEEGGLVIGVCNGFQSLL
                     RMGMLPNRDAADGGGTWAKEASLVHNESGRFVDAWVRVEFEPASRCAWTKGLAGRALP
                     VRHGEGRFLAADAATLGAIEAEGLVALRYDPAENPNGSEHHIAGICDPTGRVLGLMPH
                     PEAFLIPENHPMRRRGSRDRPGIELFENGVKAARAAV"
     gene            complement(2497..>2708)
                     /locus_tag="VFL04_02115"
     CDS             complement(2497..>2708)
                     /locus_tag="VFL04_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838526.1"
                     /translation="LFSEEPGRFLVSVRPEDRERFERSVGPAQYLGEVTREARVEASL
                     AGESVISADLGALERAFKTAIVQGE"
BASE COUNT          460 a          867 c          884 g          497 t
ORIGIN      
        1 ggactcgtga agaagggcga gatccagagg gcgttcaccc cgaggcgggc caggtaatcc
       61 agcttgccgg tgatgcccgg caggtcgccg atcccgtcgc cgttggaatc ggcgaagctg
      121 cgaggataga tctggtagaa aaccgcctct tcccaccact tgcccatgtg ccactccttt
      181 ggtcctgcgg gccccggccc gatcggcgtc cgaggccctc agcctccgcc tttcgccagc
      241 atgatctcca cctcggcctc acgggacgcg tgggcctggc aggcgagtct gatcgtcccg
      301 tccccggtcc cggacgaggg gggaatcgcg ccggatgccc ggatggcatc gaggcgctcg
      361 gcctcacgcg gcccgatggg ggagaggccg gatgcccccg acaggaccct gagcctacag
      421 gtcccgcaga gggccttccc cccgcagtcg tgcctgatct cgacgccggc gctgagaagg
      481 ctgttgagaa tcgaaaacgc ggggctggtc tcgacctcga ccccgatcct cggaatccgg
      541 atgcggccca tcgctcgcct attccgccgc gcagattcgg tcgcggccac tctgcttcga
      601 ctggtagagg gcacggtcgg cgcggtcgat gagggatttg ccggagatgt cccggccggg
      661 gatgtactgg gctagcccaa gggagaccgt gacccagagc ctttcgccct cgtacaccga
      721 tggagtctcg gctatggtct tgcggatgcg ctccgcgatg atgagcgccc gctccctgtc
      781 ggtgtccggc agaaggacgc agaactcctc accgccatac cgcgccgcga gatccatgcc
      841 cctgatgcag gcctgcagca gcttggccac ctgcttgagc accgcgtccc cgcagttgtg
      901 gccatagaca tcgttgaatt tcttgaagaa gtcgatgtcg atcatgatga cggagagggg
      961 gcggccgctc atcgacgagt gctccatctt ctcgatgagc acggtgtaga aatagtgctt
     1021 catcttgagc cgggtcatca tgtcggtggt cgtcatctcg aagaggaaga cgttgtggat
     1081 ggcgatggcg gcaagggtgg cgatgttgag gacgtactcc ttctcgccct cctcgaagtc
     1141 cgaatcgtcg atcctgtccc cgaggacgat caggccgttg acggcgccct tggcccgcag
     1201 ggggacgagg agggttggct tgagggaggc gaggccctcg atgcccttgg tcgagccaat
     1261 gcgcgccatg gcctcggcca ttgtgaagca gcggttgctg tgggtgaaaa ggacgatgag
     1321 ctcgttgccc tcggggatcg agtagttgac gctgtggtct atgtcgaagc ccttgtagtt
     1381 gcggtgaagg ctgaagtagg tggcgtcgag gcccttcttg gcgaaaaggc ccgccttgag
     1441 gaccttcatc tgtcccatga tggtgaggag gatcgagtcg atcagtatat ggtagtcgag
     1501 ggtcgagttg aggctcttgg acacctcaag aagctggcgc aggtcgtaga tctgtttttc
     1561 gtattcttcg cggaccagat caaggtcgct cgtttccggc atcggcgggg actcccactg
     1621 gagggatttt tcccaattat acggatttga aaccgcgtgc gccagagagc ccgggcgggg
     1681 tggggaggcc gaaacccgat atcgggcaag agaccccggc tcggatctca gactgccgcc
     1741 cttgccgcct tcaccccgtt ctcgaaaagc tcgatgcccg gcctatcccg gctccccctc
     1801 cttctcatcg gatggttctc ggggatcagg aaagcctcgg ggtggggcat gaggcctagc
     1861 acacgccccg tcgggtcaca gatcccggct atgtgatgct ccgatccgtt ggggttctcg
     1921 gcggggtcgt agcgcaaggc cacgaggccc tcggcctcga tggccccgag ggtcgccgcg
     1981 tccgccgcca ggaaacggcc ttctccgtgc cgcaccggca gggccctgcc cgcaaggccc
     2041 ttggtccaag cgcagcggct ggccggctcg aattcgaccc tcacccaggc gtcgacgaaa
     2101 cggcccgact cgttgtggac gaggctggcc tctttggccc atgtcccgcc tccatcggcg
     2161 gcgtcgcggt tcggcagcat gcccatgcgc agaagggact ggaagccgtt gcacacgccg
     2221 atgacgaggc ccccctcctc gacaaagcgc aggagggccg ggcggagtct ccggcggcag
     2281 agcatggaca cgacctgacc cgagccgaga tggtcgccga aggaaaagcc cccggggaaa
     2341 gcgagaatcc gcgccgacct gaggcgttct ggctcagcca ggatgtccga gagatggacc
     2401 ctctctacct ccgcgccggc gagggcaaag gcccttgcga gttcggcgtc ggcgttgaag
     2461 ccgaatcctg cgatgacgat tgccttcaca tccatctcat tccccctgga cgatggcggt
     2521 cttgaaggcc cgctccagtg caccgagatc ggcgctgatc accgattctc cggccagact
     2581 cgcctcaacc ctggcctccc ttgtcacctc gcccaggtac tgggcgggcc caacactgcg
     2641 ctcgaacctc tccctgtcct ctggccttac ggacaccagg aagcgtccgg gctcctccga
     2701 gaacaggg
//
LOCUS       DASSQV010000121         4082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2394132, whole genome shotgun sequence.
ACCESSION   DASSQV010000121 DASSQV010000000
VERSION     DASSQV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2394132"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VFL04_02120"
     CDS             complement(<1..1277)
                     /locus_tag="VFL04_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM family putative metalloenzyme
                     maturase"
                     /protein_id="HET7838527.1"
                     /translation="MSPTGQAPDRPFLPYPSRLIVETSSRCNLECIMCVKRSQQAPEG
                     ELDLALFRRLAPAFPRLESLVLNGIGEPLLHPGLESMVALARSSMPASARIGFQSNGS
                     LMDQARARALIEAGLDTACISADAVTPRLFASIRRGARLSSAESAIAILDAARRDARA
                     SGFRLGLEFVAMRSNLAELPPLVSWAAERGVDFILVSQLFPYERAFAADAAYDPHLDT
                     ALSLREKYRHRARAIGQRLEDYRRVFMKYDKDEGELALVGLVDEMAQEALAAGISLRI
                     EGLLERDDARAEVSARFFAEAERVAEGLGLSLVLPALVPRSRRECEFVEGGSAFVSWT
                     GEVHPCYFLWHRYACYIDGKEKQVQSRSFGSLAESGILSIWQSEAFSAFRRNVMRYDY
                     PYCFNCGFALCDYVEGGQFETDCHLNEEPCAACL"
     gene            complement(1274..2386)
                     /locus_tag="VFL04_02125"
     CDS             complement(1274..2386)
                     /locus_tag="VFL04_02125"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7838528.1"
                     /translation="MSSDEDLGGASWELGLGARMRRHYERFPYPKYSLLGSIRSRDAY
                     IFSLEALWAHFRGRRIRDPRAERVLLAGAGSFLPYPVCVANPRSRVVALDLSASSLAR
                     ARLHALRHLRFNLQCVAGDLMDPALAPGPYKYIDSFGVLHCIPDFSAALAALRDRLDE
                     GGILRVMVYSRDGRAQVEEVRSRCAERGITRPAEVRRLARGDPELRSLMRESFELGFD
                     EGIADAFLIPYARTFVVDEVLEALAETGLELLGFGHEGALADPRRELARIRKLEAKRE
                     FPTNLCFYAGRPMEAPKPPAWIELNPLLRNAVRFTPLGSVRLPTKFGPKNPPIDAEAR
                     RFLSRFVRPVAAASLSSSERAQARAWARLLVLMEYE"
     gene            2499..2891
                     /locus_tag="VFL04_02130"
     CDS             2499..2891
                     /locus_tag="VFL04_02130"
                     /inference="COORDINATES: protein motif:HMM:NF007714.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HET7838529.1"
                     /translation="MEAMDKRRLERELSTLRIMIAIHCRARHAARPRAQAGLCAECLE
                     LAEYAALRLGRCPWGEAKPVCAKCEIHCYLPEMRSRVREVMRYAGPRMLFAHPVLGLR
                     HLLAGGIVRQRGGKGRPGRARPDPEAPR"
     gene            complement(2921..3730)
                     /locus_tag="VFL04_02135"
     CDS             complement(2921..3730)
                     /locus_tag="VFL04_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7838530.1"
                     /translation="MVPVGLFVVSVIAFMFVALSSSESWLSMIIMFALMCSACVASVV
                     GFAFSAIAGASLYHLVSTPVEVVKILLWSSIAIQLYSVIQLWQYIKWRRLIPYLAGGF
                     VTVGPFCWLVLHMSAGAYLLAIGLFIAAYGVYRLFSSPKAVQVSKRTGLFIDVATGAM
                     GGITGPFAAFPGAAIAIWCCMRGWDKVEQRSVFQPYILIMQVVTLGIFTMMGGSSSLN
                     FSYALFAVPAVFGCHIGLLLFKRLSNSQFNRLVSCFLIISGISMIAKMLGR"
     gene            complement(3830..>4082)
                     /locus_tag="VFL04_02140"
     CDS             complement(3830..>4082)
                     /locus_tag="VFL04_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7838531.1"
                     /translation="NRITGQVKEGSAQMLAGSHGVLDEMNRLTDMTREITDRMNEMAQ
                     GTSQITAVMGRVSEVSEQNRERLAVLKKEVSWFRLGARR"
BASE COUNT          685 a         1312 c         1501 g          584 t
ORIGIN      
        1 aggcaggccg cgcagggctc ctcgttgagg tggcagtcgg tctcgaactg cccgccctcc
       61 acgtagtcgc agagggcgaa gccgcagttg aagcagtagg gatagtcgta gcgcatgacg
      121 ttccggcgga aggccgagaa ggcctcactc tgccagatgg acaggatgcc ggactcggcg
      181 agggagccga agctccggct ctggacctgc ttctccttgc cgtcgatata acaggcgtag
      241 cggtgccaga ggaagtagca ggggtggacc tcccccgtcc atgagacgaa ggcgctcccg
      301 ccctcgacga actcgcactc gcggcggctc ctcggcacca gggcgggaag gaccagggag
      361 agccccaggc cctcggccac gcgctcggcc tcggcgaaga agcgggcgct cacctcggcc
      421 cttgcgtcgt cgcgttcgag gagcccctcg atccgaagcg agatgccggc ggccagggcc
      481 tcctgcgcca tctcgtccac gagcccgacc agggctagtt cgccctcatc cttgtcgtac
      541 ttcatgaaca cgcggcggta gtcctcgagc ctttgcccga tggcccgggc ccggtggcgg
      601 tacttctcgc gcagggagag ggcggtgtcg agatgggggt cgtaggcagc gtcggcggcg
      661 aaggccctct cgtaggggaa gagctggctc acgaggatga agtcgacccc gcgctcggcc
      721 gcccacgaga cgaggggcgg cagctcggcg aggttggacc gcatggccac gaactccagg
      781 cccagcctga agcctgaggc tcgggcatcc cgcctcgccg cgtcgaggat ggcgatggcg
      841 ctctcggccg acgagagccg ggctccacgg cggatcgacg cgaagagccg gggcgtgacg
      901 gcgtcggccg agatgcacgc cgtgtcgagc ccggcctcga tgagggccct cgccctggcc
      961 tggtccatga gggagccgtt gctctggaag ccgatgcggg ccgaggccgg catcgaggat
     1021 cgggcgaggg cgaccatgga ctcgaggccg ggatggagga ggggctcgcc gatcccgttg
     1081 agcacgaggg actcgaggcg ggggaaagct ggcgcgaggc ggcggaagag ggccaggtcg
     1141 agctcgccct cgggtgcctg ctggctgcgc ttgacgcaca tgatgcactc gaggttgcag
     1201 cgcgagctcg tctccacgat gaggcgggac gggtatggca ggaagggccg gtcgggggcc
     1261 tggccggtcg ggctcactcg tattccatga ggacgaggag cctggcccat gccctggcct
     1321 gggccctctc cgaagacgag agcgaggctg cggcgaccgg ccggacgaag cgcgagagga
     1381 agcgccgggc ctcggcgtcg atcggcgggt tcttggggcc gaacttggtc gggagccgga
     1441 ccgagcccaa gggcgtgaag cgcacggcat tcctgagcag ggggttgagc tcgatccagg
     1501 ccgggggctt gggggcctcc atgggacggc ccgcgtagaa gcagaggttc gtggggaact
     1561 cgcgtttggc ctcgagcttc ctgatgcgcg cgagctccct ccgcgggtcg gccagggccc
     1621 cctcgtggcc gaagccgagg agctcgagcc ccgtctcggc cagggcctcc agcacctcgt
     1681 cgaccacaaa ggtcctcgca taggggatga ggaaggcgtc ggcgatgccc tcgtcgaagc
     1741 cgagctcgaa gctctcgcgc atgaggctgc ggagctcggg gtcgccgcgc gctaggcggc
     1801 gcacctcggc cggcctcgtg atcccgcgct cggcgcagcg gctcctgacc tcctcgacct
     1861 gggcacggcc gtcccggctg tagaccatga cgcggaggat cccgccctcg tcgagcctgt
     1921 ccctgagggc agcgagggca gccgagaagt cggggatgca gtggaggacc ccgaaggagt
     1981 cgatgtactt gtaggggccg ggggccaggg ccgggtccat gaggtcgccc gccacgcact
     2041 ggaggttgaa gcggaggtgg cggagggcgt ggagcctcgc ccgcgcgagg gatgaggccg
     2101 agaggtcgag ggcgacgacc ctcgagcggg gattggccac gcagacggga taggggagga
     2161 aggatcctgc ccccgcgagg aggacgcgct cggccctggg gtcccggatc cgccggccgc
     2221 ggaaatgcgc ccagagggcc tcgaggctga agatgtaggc gtcgcggctc cggatggagc
     2281 cgaggaggct gtacttgggg taggggaagc gctcgtagtg gcgcctcatc ctcgcgccga
     2341 ggcccagttc ccacgaggct ccgcccagat cctcatcgct gctcatcgct gtagcagata
     2401 gcatggcccg ggctccctgt caacgaaggg gccccgcccc aggtcgggcg ccggactctt
     2461 gaaagccggg ccgagccggg gcgatactca tgggagacat ggaagccatg gataagcgcc
     2521 gcctcgagcg cgagctgtcg accctcagga tcatgatagc gatccactgc cgcgcaaggc
     2581 acgccgcgcg cccccgggcc caggccgggc tctgcgctga gtgcctcgag ctggccgagt
     2641 acgccgcgct ccgcctcggc cgctgcccct ggggcgaggc caagcccgtc tgcgccaagt
     2701 gcgagatcca ctgctatctg cccgagatgc gctcccgggt gcgcgaggtc atgcgctacg
     2761 ccgggccgcg gatgctcttc gcccatccgg tgctcgggct gcggcacctc ctggccggcg
     2821 gcatcgtccg ccagcgcggg ggcaaaggcc gaccgggcag ggcgcgccca gacccggagg
     2881 cgccgcgcta gccgcccctc taggggcgcc cgcccggggc tcagcgcccg agcatcttcg
     2941 ctatcatcga gatgcccgag atgatgagga agcaggacac gaggcggttg aactggctgt
     3001 tgctcaggcg cttgaacagc aggaggccga tgtggcagcc gaagacggcg gggacggcga
     3061 acagggcgta ggagaagttc aggctgctcg agccccccat catggtgaag atgccgaggg
     3121 tcaccacctg catgatgagg atgtagggct ggaacacgct gcgctgctcc accttgtccc
     3181 agccgcgcat gcagcaccag atggcgatgg ccgcgcccgg gaaggcggcg aatggcccgg
     3241 tgatcccccc catggccccg gtggcgacgt cgatgaagag ccccgtgcgt ttgctgacct
     3301 ggacggcctt ggggctgctg aacagccgat acacgccgta ggcggcgatg aagagcccga
     3361 tcgcgaggag ataggccccg gcggacatgt gcaggacgag ccagcagaag gggccgaccg
     3421 tgacgaagcc tcccgcgagg taggggatga gccgccgcca cttgatgtac tgccagagct
     3481 ggatcacgct gtacagctgg atcgcgatgg agctccacag gaggatcttc accacctcga
     3541 cgggggtgga gacgaggtgg tagaggctcg cgccagcgat ggccgagaag gcgaagccca
     3601 cgaccgaggc gacgcaggcc gagcacatga gcgcgaacat gatgatcatc gagagccatg
     3661 actcgctgga tgacagcgcc acgaacatga acgcgatcac cgagacgacg aacaggccaa
     3721 ccggcaccac ggcatctttc cgtgtctgcg gaatgggata cgccataagg tcccctcctc
     3781 caaatggatt caagccgggg agaagtcgcg atggccttga gcgcggacct tagcgccgcg
     3841 cgccaagcct gaaccaggat acttctttct tcaggacagc caagcgttcc cggttctgct
     3901 cgctgacctc ggacacccgg cccattacgg ccgtgatctg gctggtgcct tgcgccatct
     3961 cgttcatgcg gtcggtgatc tcgcgggtca tgtcggtcag gcggttcatc tcgtcgagga
     4021 ctccgtggct gcccgcgagc atctgggccg acccttcctt gacctggccc gtgatcctgt
     4081 tg
//
LOCUS       DASSQV010000122         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1274372, whole genome shotgun sequence.
ACCESSION   DASSQV010000122 DASSQV010000000
VERSION     DASSQV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274372"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VFL04_02145"
     CDS             <1..730
                     /locus_tag="VFL04_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7838532.1"
                     /translation="LENVMESFPGSVFWKDRNLVYLGCNLAEARSAGLASPRDIVGKT
                     DFDLGWSEGEAEFFRASDMRVLESGAPVLNIAEAHMYGGEPSWMETSKAPIFDSAGRI
                     SGILGVSLDITERRRAEMALRESEASLERAEMVAKIGNWKLMLDTGEIVGSAGACSIY
                     GVQRDRMSLREIQAMVLPEYRSEGDRALAELIERDAPYRLKAKIRRPSDGRVLDILST
                     ATYDRRQRVVHGVVQDVTDHKAAL"
     gene            complement(725..>2644)
                     /locus_tag="VFL04_02150"
     CDS             complement(725..>2644)
                     /locus_tag="VFL04_02150"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HET7838533.1"
                     /translation="QCYPFMIRSAAVGAEAKARFLVPTLHFRYSRRKLVRDLAGFMAA
                     SFGSPAREVALAVRAAEAAQLGFERELAAEGRRVMAELPAERRAFVVLGRPYNAGDPE
                     LNLGLVRKLIDLDVLPIPLDFLPLEEELEGVYRDYPMVCWPNAQRILAGARIVARSEG
                     LHAVFITNFRCGPDSFLSHYLREELRGKPQLQLEVDEHGADAGMITRCEAFIASLRAG
                     RRAEPARSPRGALARSTPSPSPGRRLYFPYMCDGAELVAAASRACGIEAEVLPMQDEA
                     ALEWGRKYTSSRECFPMICTLGSFLKKLHEEGVDPARVSFFMPSHGGPCRFGQYEKLQ
                     RVILSRLGFDEVEIVSPSNKRSYADFTKGHGARFRLCVWKGLVAGEILAMLRQECRPY
                     EARPGASAEAYGRGLAALVSSVERGAGDLVRVLRATAEEFSAIPRLDLPRKPVVAIIG
                     EIFMRDNPFCSGFLRDRLEALGVETVMTPIRDWLELSSLNYRKLSRWEGRIFDAAKAQ
                     AQLFLQRRIAQGIERRFAGLVAAERIVPIEEVMELCGTYIHRDYSGEPPLALGAAASL
                     ARAGISGFVSVIPFTCLPGTVVASLCGAFRADHGGVPWLNIAFDGQDDIGTETRLQAF
                     VHQAREFARAHAAGS"
BASE COUNT          413 a          892 c          974 g          365 t
ORIGIN      
        1 cctggagaac gtcatggaga gctttcccgg gagcgtcttc tggaaggaca ggaacctcgt
       61 ctacctcggc tgcaacctgg ccgaggcccg cagcgccggc ctggcctcgc cccgcgacat
      121 cgtcgggaag accgatttcg acctcggctg gagcgagggc gaggccgagt tcttccgggc
      181 ctcggacatg agggtccttg agagcggggc gcccgtcctc aacatcgccg aggcccacat
      241 gtacgggggc gagccgagct ggatggagac gagcaaggct ccgatcttcg acagcgccgg
      301 gaggatctcg ggcatcctcg gggtttcgct cgacatcacc gaacgccggc gcgccgagat
      361 ggccctccgc gagagcgagg ccagccttga gcgggccgag atggtggcca agatcggcaa
      421 ctggaagctc atgctcgaca cgggggagat cgtcggctcg gccggggcct gcagcatcta
      481 cggggtacag agggaccgca tgagcctgcg ggagatccag gccatggttc tgcccgagta
      541 ccggagcgag ggcgatcggg ccctcgccga gctcatcgag cgcgacgcgc cctacaggct
      601 gaaggcgaag atccggaggc cctcagacgg cagggtcctc gacatcctct cgaccgcgac
      661 ctacgacagg cggcagcggg tcgtgcacgg ggtggtgcag gacgtcaccg accacaaggc
      721 cgccctatga tccggccgcg tgggcgcgcg cgaactccct cgcctggtgg acgaaggcct
      781 ggagcctggt ctcggtgccg atgtcgtcct ggccgtcgaa ggcgatgttg agccagggga
      841 cgcccccgtg gtcggcgcgg aaggccccgc agagcgaggc cacgacggtg cccggcagac
      901 aggtgaaggg gatcaccgag acgaagcccg agatcccggc gcgcgccagg ctggccgccg
      961 ccccgagggc cagggggggc tcgcccgagt agtcgcggtg gatgtaggtc ccgcagagct
     1021 ccatgacctc ctcgatgggc acgatgcgct cggcggcgac gaggcccgcg aagcggcgct
     1081 cgatcccctg ggcgatcctc ctctggagga agagctgggc ctgggccttc gcggcgtcga
     1141 agatccgccc ctcccagcgg ctcagcttgc ggtagttgag cgacgagagc tcgagccagt
     1201 cccgtatcgg ggtcatcacc gtctccaccc cgagggcctc gagcctgtcc cgcaggaagc
     1261 cgctgcagaa ggggttgtcg cgcatgaaga tctccccgat gatcgcgacc accggcttgc
     1321 gcggcaggtc gaggcggggg atggcggaga actcctcggc cgtggcgcgc aggaccctca
     1381 ccaagtcccc ggcccctcgc tcgaccgagc tcaccaaggc cgccaggccg cggccgtagg
     1441 cctcggcgct cgcgcccggc ctcgcctcgt agggcctgca ctcctgccgg agcatcgcca
     1501 ggatctcgcc ggccacgagg cccttccaca cgcagaggcg gaagcgcgcg ccgtgtcctt
     1561 tggtgaagtc ggcataggag cgcttgttgg agggcgagac gatctcgacc tcgtcgaagc
     1621 cgagcctcga gaggatgacc cgctggagct tctcgtactg gccgaagcgg cagggcccgc
     1681 cgtggctggg catgaagaag cttacgcggg ctgggtcgac cccctcctcg tgcagcttct
     1741 tgaggaagct cccgagggtg cagatcatgg ggaagcactc tcggctcgag gtgtacttcc
     1801 tcccccattc aagcgccgcc tcgtcctgca tgggcaggac ctcggcctcg atcccgcagg
     1861 cccgtgaggc cgcggccacg agctcggctc cgtcgcacat gtaggggaag tagagcctgc
     1921 ggcccggcga ggggctcggc gtcgaccgcg cgagggcccc ccgcgggctc cgcgcgggct
     1981 cggccctccg cccggccctg aggctcgcga tgaaggcctc gcagcgcgtg atcatgcccg
     2041 cgtcggcgcc gtgctcgtcc acctcgagct ggagctgggg cttgccccgg agctcctcgc
     2101 ggaggtagtg ggagaggaag gagtcggggc cgcagcggaa gttcgtgatg aagacggcgt
     2161 ggaggccctc gctcctcgcg acgatcctcg cgcccgcgag gatccgctgc gcgttgggcc
     2221 agcagaccat ggggtagtcc cggtagacgc cctccagctc ctcctcgagg ggcaggaagt
     2281 cgagggggat gggcaggaca tcgaggtcga tcagcttccg gacgaggccg aggttgagct
     2341 cggggtcgcc ggcgttgtag gggcgtccga gcacgacgaa ggcgcggcgc tccgccggca
     2401 gctcggccat caccctgcgg ccctcggccg cgagctcgcg ctcaaagccc agctgggcgg
     2461 cctcggcggc gcgcacggcg agggccacct cgcgggccgg gctgccgaag ctggcggcca
     2521 tgaagccggc caggtccctg acgagcttgc gccgagagta gcggaagtgg agggtcggca
     2581 cgaggaagcg cgccttggcc tcggccccga cggccgcact gcggatcatg aaggggtagc
     2641 actg
//
LOCUS       DASSQV010000123         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_279180, whole genome shotgun sequence.
ACCESSION   DASSQV010000123 DASSQV010000000
VERSION     DASSQV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279180"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..2038)
                     /locus_tag="VFL04_02155"
     CDS             complement(38..2038)
                     /locus_tag="VFL04_02155"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HET7838534.1"
                     /translation="MKLELNRGVDILADLGGVAAIAEARKLLEAKTDKANFDKLSTIK
                     NPEAIIRIANAVALMEPSSLWINTGSASDMSLAREMAIATGEEFKLRLEGHSCHFDLP
                     EDQGRMVDKTFYIVNEGEEVSAAANRMIRREATSYIDSYFRGIMKGKQMLLSFWNRGP
                     VGAKAAIPAIMISDSWYVVHAGNLLYPSDFSSFDAEVKRAGVFFTNTHSMGRFSSEDI
                     PKARIFMDRSWLTTFSMFCTYAGNTLMLKKGNHRFAVDLCTYFRVGSQLSEHMFITGL
                     TGPKGRKTFFAGAAPSGCGKTTTAMVGTDFIGDDLAQIWIEDDGTMRAVNPEIGIFGI
                     VEDVNQEGDPLLMDCLRGRKKTEVLWSNVLIDDAGVPHWTGHGEPTPAHGRNWLGDWT
                     PGKLDACDKPIPMSNPNARITLSCRAMDNYNETAATDPAGCPISVITYSGRDPDTMPP
                     VWVAKSPDHGVVIGASILSAATATEVTVAGAATAKTIKREPWANAPFTPGPVGDYMYA
                     QFEFFNSSRLTKKPLMAGLNYFLTHGARGGEAGSKKLLGEKRDVKAWLTWLERRAHGE
                     VGAIETPIGYIPRYEELKAVFSETIGKEYPKELYTKQFSFYIDNILSRLELQEAASEK
                     DGNCPSRIFEVYAEQRAGLLALKAKHGPVVSPEQLAAEGCRK"
     gene            complement(2339..>3103)
                     /locus_tag="VFL04_02160"
     CDS             complement(2339..>3103)
                     /locus_tag="VFL04_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838535.1"
                     /translation="DEDLAADGFEGFCVSQFFPNHITLLVGSASQFVYASDHATLRRK
                     GRMTDRQKARRAQLDRAYRPDSRALELSFARTADEGLRVLSDGCRPTLRLWTDEHPAY
                     PRAIRGSPCLAALLDLGRLRHLTVPSRAPRTRANPLFPVNYLDRELRKDLREHVRETV
                     CFGRNVNRQMERLSVYLWWHNFIKPHRAREGPRSHAEAAGYDGERIREGLRWIWLRRA
                     WLSLTDLTWPMLDSWLRSRRTPLRPGPDYLPRYASA"
BASE COUNT          460 a          918 c         1146 g          579 t
ORIGIN      
        1 gtggatcctc tagaccggcg gcccagcggg actcgtccta cttcctgcag ccctcggcgg
       61 ccagctgctc cggcgagacg acgggcccgt gcttggcctt cagggcaagg agcccggccc
      121 gctgctcggc gtagacctcg aagatccttg aggggcagtt gccgtccttc tcggatgcgg
      181 cctcctggag ctcgagcctc gagaggatgt tgtcgatgta gaaggagaac tgcttcgtgt
      241 agagctcctt cgggtattcc ttgccgatcg tctcggagaa cacggccttg agctcctcgt
      301 agcgggggat gtagccgatg ggggtctcga tcgccccgac ctcgccgtgg gcccggcgct
      361 cgagccaggt gagccaggcc ttcacgtccc gcttctcccc gagcagcttc ttgctgccgg
      421 cctcgccccc gcgggcgccg tgggtgagga agtagttcag gcccgccatg aggggcttct
      481 tcgtgagccg gcttgagttg aagaactcga actgggcgta catgtaatcg ccgacggggc
      541 caggggtgaa gggcgcgttg gcccagggct cgcgcttgat ggtcttggcg gtggcggctc
      601 cggccaccgt cacctcggtc gccgtggcgg ccgagaggat ggaggcgccg atgacgacgc
      661 catggtcggg gctcttggcc acccagaccg ggggcatggt gtcggggtcg cggccggagt
      721 aggtgatcac cgagattggg cagccggcgg ggtcggtggc cgcggtctcg ttgtagttgt
      781 ccatggccct gcagctcagg gtgatgcggg cgttggggtt ggacatcggt atgggcttgt
      841 cgcaggcgtc cagcttcccg ggcgtccagt cgccgagcca gttgcggccg tgggcgggcg
      901 tcggctcgcc gtggcccgtc cagtggggca cgccggcgtc gtcgatgagg acgttggacc
      961 agaggacctc ggtcttcttg cgcccccgga ggcagtccat gaggagggga tcgccctcct
     1021 gattcacgtc ctcgacgatg ccgaagatgc cgatctcggg gttgacggcg cgcatcgtgc
     1081 cgtcgtcctc gatccagatc tgcgccaggt cgtcgccgat gaagtcggtg ccgaccatgg
     1141 ccgtcgtggt cttgccgcag cccgagggcg cggcgccggc gaagaaggtc ttcctgccct
     1201 tggggccggt gaggccggtg atgaacatgt gctcggagag ctggctgccc acgcggaagt
     1261 aggtgcagag gtcgacggcg aagcggtggt tgcccttctt gagcatgagg gtgttgccgg
     1321 cgtaggtgca gaacatcgag aaggtggtga gccagctgcg gtccatgaag atcctggcct
     1381 tcgggatgtc ctcgctggag aagcggccca tgctgtgggt gttggtgaag aagacccccg
     1441 cccgcttcac ctcggcgtcg aagctggaga agtccgaggg gtagaggagg ttgcccgcgt
     1501 gcacgacgta ccaggagtcg ctgatcatga tggcggggat ggcggccttg gccccgacgg
     1561 ggcccctgtt ccagaaggag agcagcatct gcttgccctt catgatcccg cggaagtagg
     1621 agtcgatgta gctcgtggcc tcgcgcctga tcatcctgtt ggcggcggcc gagacctcct
     1681 cgccctcgtt cacgatgtag aaggtcttgt cgaccatgcg gccctggtcc tcggggaggt
     1741 cgaagtggca ggagtggccc tcgagcctga gcttgaactc ctcgcccgtg gcgatggcca
     1801 tctcgcgggc gaggctcatg tccgaggccg agcccgtgtt gatccagagg ctggagggct
     1861 ccatgagggc gacggcgttc gcgatccgga tgatggcctc ggggttcttt atcgtgctga
     1921 gcttgtcgaa gttggctttg tcagtcttgg cctcgaggag cttgcgcgcc tcggcgatgg
     1981 ccgcgacccc gccgaggtca gcgaggatgt cgacacctct gttgagttcc agtttcattg
     2041 tcctctccgc gtatgtggct ggtgaagatt ccgagacggt cggcctgggc ccccctcatg
     2101 gaaggctgcc gctcgtgttc gggcagggaa cgtgcagttc ttacacctga tgtatcagca
     2161 gactactacg ggtcttgggg accgtcaagt tcaaatcgtg aaatagacat tcagatactg
     2221 aaacgtcaaa ctcagttcct cgaatcttgt tggcctactc cacagccctc cgcgaagagc
     2281 cgcttttcgt gggtggaatc agggagattc gcctgtcgcc cctcaaaatc gttcccgctc
     2341 aggcgctggc atagcggggc aggtagtcgg ggcccggccg caggggcgtc ctgcgcgagc
     2401 gcagccagga gtcgagcatg ggccaggtca ggtcggtgag cgacagccaa gcccggcgga
     2461 gccagatcca tctgaggcct tccctgatcc tctccccgtc gtagccggcc gcctcggcgt
     2521 ggctgcgcgg cccctccctt gcgcggtgcg gcttgatgaa gttgtgccac cacaggtaga
     2581 ccgacaagcg ctccatctgg cggttgacgt tccgaccgaa acagacggtc tcgcgcacgt
     2641 gctcgcgcag gtccttgcgc agctcgcggt cgaggtagtt gacggggaac agggggttgg
     2701 ccctggtcct gggggcgcgg gagggtacgg tgaggtggcg caggcgtccg aggtcgagga
     2761 gggccgcgag gcagggcgag cctcggatcg cgcgggggta ggcggggtgc tcgtcggtcc
     2821 agagcctgag ggtgggccga caaccgtcag agaggacgcg caggccctcg tcggccgtcc
     2881 gcgcgaagga cagctcgagg gcacgggagt cgggccggta ggcgcggtcg agctgggccc
     2941 gacgcgcctt ctggcggtcg gtcatccggc ccttcctgcg gagggtggcg tggtcgctgg
     3001 cgtagacgaa ctgggaggcg gagccgacca ggagcgtgat gtggttgggg aagaactggg
     3061 agacgcagaa gccctcgaag ccgtcggcgg cgaggtcctc gtc
//
LOCUS       DASSQV010000124         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1274582, whole genome shotgun sequence.
ACCESSION   DASSQV010000124 DASSQV010000000
VERSION     DASSQV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274582"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VFL04_02165"
     CDS             <1..503
                     /locus_tag="VFL04_02165"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET7838536.1"
                     /translation="STEALGAIENPKGLPPPGCLVGGRARGELIGGNLSLIAATMGTP
                     YELDARGKILFLEDIGERPYRVDRMLTQLALAGKFAQCAGVVLGDWNDCGPEEGKPSL
                     LLAQIFRDIIGPFGKPCIYGIKAGHCSPTATLPFGVEASLDADAAGLELVEAAASSSI
                     IVGGRS"
     gene            500..2329
                     /locus_tag="VFL04_02170"
     CDS             500..2329
                     /locus_tag="VFL04_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HET7838537.1"
                     /translation="MRSQRKGSLSSIVIASRLGIVAKLALVSAVTLCALGAILVGTHS
                     AIAILSGNVDELQRRQAGILQDSGDLQIDCYEAQVALYRATIQAIGAGKGLAASEVTI
                     VRDNLNAAKALVAKLAAAPEVQLGGAADSLVSSFESYSADVETLLKSANVRNNEGLLA
                     VTDANTHFGSLSKRVSMIASTLRMNGMDASDRSRSASMSARTVVDLVVFIDVVVIVAL
                     TVLIILSITRPLRGLLATVVRAGTGDLTIATGAQGGDEVARISQGVDTLVSSLRGLVG
                     TVKERVATLEDTGRHLASTMEETGASVVQINSNIANTRGQLDEQSAAVDQVSATIEEL
                     TRSIEALSGMIGNQTGGIARSSASIERVIHGIDSVADQADEAGRNSKLLSAEGADGKA
                     RIDEVSEAVGAIVRYSQNLNEAAALITEIADRTNLLAMNAAIEAAHAGESGKGFSVVA
                     DEIRKLAEQSTSQARDIASDLGKVSTSIEAVRASAGQAVTSFGSILARAGALEEAVKE
                     IERAVSEQREGGKQVLEGLGTLRELSGKITRGVEEMASGNASILDQVGRLRNANHQVV
                     RNNEEVTIGTKEINEAVSGTMELCSRNTELIAEVKRAADLFSV"
     gene            2360..>2884
                     /locus_tag="VFL04_02175"
     CDS             2360..>2884
                     /locus_tag="VFL04_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7838538.1"
                     /translation="MTMHGDGATGHLIFDGCDTVELAREYGTPLYVMSETAIRERLSE
                     VRRTFLDRYPGSYAVYASKAFQTLEMCRIVQSEGLGLDVVSGGELYAARRAGFPMEKV
                     LFHGNSKTPEELRMAVEYGVGRVVVDNLDELALLDRIARERSTRAARILFRITPGVDS
                     HTHEFVSTGRLDSKF"
BASE COUNT          521 a          998 c          961 g          404 t
ORIGIN      
        1 cgtccaccga ggccctcggc gccatcgaga acccaaaggg cctgcctcct ccgggctgtc
       61 tcgtgggcgg cagggcgagg ggggagctga tcggcggaaa cctctccctc atagccgcga
      121 ccatgggcac cccctacgag ctcgacgcgc ggggcaagat cctcttcctc gaggacatag
      181 gggagaggcc ctaccgcgtg gacaggatgc tcacccagct cgccctcgcg ggcaagttcg
      241 cgcaatgcgc cggcgtcgtc ctcggcgact ggaacgactg cggccccgag gaggggaagc
      301 catcgctgtt gctcgctcag atcttccgtg atatcatcgg gccattcgga aaaccctgca
      361 tctacggcat caaagcggga cactgcagcc ccacggccac cttgcccttc ggcgtcgaag
      421 ccagcctcga cgccgacgcc gcggggctcg agcttgtcga ggccgcggcg tcctccagca
      481 tcatcgtcgg agggagatca tgagaagcca gcggaagggg agtcttagct cgatcgtcat
      541 cgcatccagg ctcggcatcg tggccaagct tgcccttgtc tcggccgtga ccctctgcgc
      601 cctcggcgcc atcctcgtgg ggacccactc cgccatcgcg atcctctcgg gcaacgtcga
      661 cgagctccag aggcgacagg ccggcatcct ccaggacagc ggggacctgc agatcgactg
      721 ctacgaggcc caggtcgccc tctaccgggc cacgatccag gccatcggcg cgggcaaggg
      781 cctggccgcc tccgaggtca cgatcgtgag ggacaacctc aacgctgcga aggccctcgt
      841 ggccaagctc gccgccgcgc ccgaggtcca gctcgggggc gctgccgatt ccctggtctc
      901 ctccttcgag agctactcag ctgacgtcga gaccctgctc aagagcgcca acgtcaggaa
      961 caacgagggc ctcctcgccg tcaccgacgc caacacccac ttcgggtccc tgtccaagcg
     1021 ggtctctatg atcgcctcga cgctcaggat gaacggcatg gacgcctcgg accgctcccg
     1081 gagcgcctcg atgagcgcca ggaccgtggt cgacctcgtg gtcttcatcg acgtcgtcgt
     1141 gatagtcgcc ctgacggtcc tcataatact gtcgatcacg aggcccctgc gcggcctcct
     1201 cgccacggtg gtcagggcgg gaacgggaga cctcaccatc gcgaccgggg cccagggcgg
     1261 ggacgaggtg gcgaggatct cccaaggggt cgacaccctc gtctcgagcc tcaggggact
     1321 cgtcggcacg gtcaaggaga gggtggcgac cctcgaggac acggggaggc acctggcctc
     1381 caccatggag gagaccgggg catcggtggt ccagatcaac tcgaacatcg cgaacaccag
     1441 gggccagctc gacgagcagt cggccgcggt ggaccaggtt tcggcgacga tcgaggagct
     1501 cacgcggagc atcgaggccc tctccggcat gatcggcaac cagaccggcg gcatcgcccg
     1561 atcctcggcc tccatcgagc gggtgatcca cggcatagac tcggtggccg accaggccga
     1621 cgaggcgggc cggaactcga agctcctctc cgccgagggc gccgacggca aggcaaggat
     1681 cgacgaggtg agcgaggccg tgggcgcgat cgtgcgctac tcccagaacc tcaacgaggc
     1741 cgccgccctc atcaccgaga tcgccgaccg gaccaacctc ctcgccatga acgccgccat
     1801 cgaggcggcc cacgcggggg agtcgggcaa gggcttctcg gtggtagccg acgagatcag
     1861 aaagctcgcc gagcagtcca cctcccaggc gagggacata gcctccgacc tcgggaaggt
     1921 gtccacctcc atcgaggccg tgcgcgcctc agccggccag gccgtgacca gcttcggctc
     1981 gatcctcgcc agggcgggcg cccttgagga ggcggtcaag gagatcgaga gggccgtgtc
     2041 agagcagagg gagggcggca aacaggtcct cgaggggctc gggaccctca gggagctgag
     2101 cggcaagatc acgaggggcg tcgaggagat ggcatcgggc aacgcgtcca tcctcgacca
     2161 ggtggggcgg ctgcggaacg ccaaccacca ggtcgtgagg aacaacgagg aggtcaccat
     2221 cggcaccaag gagatcaacg aggccgtgtc cgggaccatg gagctctgct cgaggaacac
     2281 cgagctcatc gccgaggtga agcgggcagc cgacctcttc tcggtctagg cgccaaggcg
     2341 ccggaaagga cggagcgtga tgacgatgca cggcgacggg gccacgggcc acctcatctt
     2401 cgacggctgc gacaccgtcg agctcgcgag ggagtacggc acccccctct acgtcatgtc
     2461 ggagacggcc atcagggagc ggctgtccga ggtcaggagg accttcctcg ataggtaccc
     2521 gggcagctac gccgtctatg cctcgaaggc cttccagacc ctcgagatgt gccggatcgt
     2581 ccagtccgag ggcctcggcc tcgacgtggt gtccgggggc gagctctacg ccgcccgccg
     2641 cgccggcttc cccatggaga aggtcctctt ccacggcaac agcaagaccc ccgaggagct
     2701 ccgcatggcg gtggagtacg gcgtggggcg ggtcgtcgtc gacaacctcg acgagctcgc
     2761 cctgctggac cgcatcgccc gggagcgctc gacccgcgcc gccaggatcc tcttccgcat
     2821 aacgcccggg gtcgacagcc acacccacga gttcgtctcc acgggcaggc tcgactccaa
     2881 gttc
//
LOCUS       DASSQV010000125         4512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_652725, whole genome shotgun sequence.
ACCESSION   DASSQV010000125 DASSQV010000000
VERSION     DASSQV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652725"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="VFL04_02180"
     CDS             complement(<1..1455)
                     /locus_tag="VFL04_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET7838539.1"
                     /translation="MRPAELLARATERLGGQAGGDPFFFSRLGERYAVIHRLFRSLYG
                     GTPDFEALLERLEALLLEKYRERPEALKASDRLREAGKRGPRGTGAEGGAEAGPEGGA
                     WYLEPGLAAYQLYLDRFAGDLAGFRERIPYLAELGVGLVHLMPFFKSPPGNNDGGYAV
                     SDYLSVDPRYGSNENLVAAAEALHARGMYLAADLVLNHCADDHPWAVAARAGDPRRRA
                     FFYAYPDRELPDRFEAAMPEVFPETAPGNFTWVPEMRAWVMTVFHDFQWDLNWSNPEL
                     FLEMLGVLLGMANLGVDLLRLDAVPYLWKRPGTSCQNLDEAHDIVRLLRACAAVVAPG
                     LAFLAEAIVQPAEIVRYLDTDEAEECQLAYHASLMACLWEALATRKPALLRLSVGGET
                     RLPRDAAWLSYARCHDDIGLGYEDAQIEAAGYSPSLHRRFLLDWYSGAFPGSTARGRL
                     FMENPATGDARISGSCASLCGLEAALAAAPGAGVP"
     gene            complement(1452..2210)
                     /gene="pgmB"
                     /locus_tag="VFL04_02185"
     CDS             complement(1452..2210)
                     /gene="pgmB"
                     /locus_tag="VFL04_02185"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HET7838540.1"
                     /translation="MDRIRAFVFDLDGVIVDTAGFHFLAGRRLARELGGELGAADNER
                     LKGVSRMASLDIILSLAGVEASPERKLELADKKNAWYREYLATMDPSGVLPGAREFVL
                     AAREAGLRTAIASASKNTSTILEATGIAGLFDAVADGHKAERAKPAPDVFLKAAEELG
                     LPPTACVVFEDAAAGIEGAIAAGMATVGIGDPDILSRADIVLPNLVGIGPAGLLGLLA
                     KAESGRGRSAGPGGAGGPGGAGAKGGPGGTRGGR"
     gene            complement(2338..3081)
                     /locus_tag="VFL04_02190"
     CDS             complement(2338..3081)
                     /locus_tag="VFL04_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7838541.1"
                     /translation="MSDPSIASIIERAFARERLRNIDRRFVHAPFLIERRLSDYIVAG
                     DREAALATLAEINARERATLASDRLRSLKNGIIGSCTIYTRAAIEGGVDSELAFTLSD
                     TFIRRIESARGEEELAHFEEGMVERFIELVLRYRTEKRDVLVSRAARIVRARIEKKVT
                     LAETAAALGVSAPYLSTQFRKKMGLSFVDFVHKMKVEEATYFLRATDLSVMEVAQMLG
                     FTYQAHFSRVFRRALGLSPTAYRKAHLVA"
     gene            3194..4495
                     /locus_tag="VFL04_02195"
     CDS             3194..4495
                     /locus_tag="VFL04_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7838542.1"
                     /translation="MQKSLVAILAIAALLVATVAVSADPLSDAFAGKFKGTVVRMSGP
                     FTDNDAVKFNDSMKSFEARTGIDVQYEGSKEFEASISIRVDGGNAPDIVDFPQPGLLE
                     SFVKKGKVIDLNKVLDMNKVKANYSQSWLDMATMTGPKGKIVAGVWGRVNGKSCVWYN
                     KKAFDAAGYKVPTTWAELVKLCDQIKKDGDTPWAIGIESGAATGWAATDWIEDIMLRT
                     TSLANYDKWVKGELKFDSPEVKKAMQTMADMWFTDGYVYGGRKAIATTFFGDAPKPMF
                     DNPPKAWLHRQGNFITSFFPNNLVAGQDYDFFYLPPIDPKYGKPVLVAGDIYAMFNDR
                     PEVRALMQYFSTGASVETWVKAGGAISPHKDSKLDWYTNYVDRKVAEMILNASSVRFD
                     GSDQMPGAVGAGSFWKHMTAWVSGSETLDQAAKAIDASWPK"
BASE COUNT          728 a         1486 c         1627 g          671 t
ORIGIN      
        1 cgggaccccg gcccccgggg cagccgcgag ggcggcctcg aggccgcaga gcgaggcgca
       61 gctgcccgag atgcgcgcgt cccccgtcgc cgggttctcc atgaagagcc tgccgcgggc
      121 cgtcgagccg gggaaggcgc ccgagtacca gtcgaggagg aagcggcggt ggagggaggg
      181 gctgtagccc gcggcctcga tctgggcgtc ctcgtagccg aggccgatgt cgtcgtggca
      241 gcgcgcgtag gacagccagg ccgcgtcccg gggcaggcgg gtctcgccgc cgacggagag
      301 cctgaggagg gccggcttcc gcgtggcgag ggcctcccag agacaggcca tgagggaggc
      361 gtggtaggcg agctggcatt cctcggcctc gtccgtgtcg aggtagcgca cgatctcggc
      421 gggctggacg atggcctcgg cgaggaaggc gagccccggg gcgaccacgg cggcgcaggc
      481 cctgagcagg cgcacgatgt cgtgggcctc gtcgaggttc tggcagctcg tccccggccg
      541 cttccagagg tagggcacgg cgtcgagcct aaggaggtcg acgccgaggt tggccatgcc
      601 gaggaggacc ccgagcatct cgaggaagag ctcggggttc gaccagttga ggtcccactg
      661 gaaatcgtgg aagaccgtca tcacccaggc ccgcatttcg ggcacccagg tgaagttacc
      721 gggggccgtc tcggggaaga cctcgggcat cgcggcctcg aagcggtcgg gcagctcgcg
      781 gtcggggtag gcgtagaaga aggcccggcg ccgcgggtcg cccgcccggg ccgcgaccgc
      841 ccaggggtgg tcgtcggcgc agtggttcag gacgaggtcg gcggcgaggt acatgccgcg
      901 ggcgtggagg gcctcggccg cggccaccag gttttcgttg ctgccgtagc gggggtcgac
      961 gctgaggtag tcgctcaccg cgtagccccc gtcgttgttg cccggcgggc ttttgaagaa
     1021 gggcatgagg tggacgaggc cgacgccgag ctcggccagg taggggatcc gctccctgaa
     1081 gccggcgagg tctcctgcga agcggtcgag gtagagctga taggcggcga gccccggctc
     1141 gaggtaccag gccccgccct cgggtcctgc ctcggctccg ccctcggccc cggtcccgcg
     1201 gggcccccgt tttccggcct ccctcagcct gtcgctcgcc ttgagggcct cggggcgctc
     1261 gcggtacttc tcgaggagga gggcctcgag ccgctcgagg agggcctcga aatcgggggt
     1321 cccgccgtag agggagcgga agagcctatg gatgaccgcg tagcgctcgc cgagccgcga
     1381 gaagaagaag gggtcgccgc cggcctggcc gccgaggcgc tcggtcgccc gggccaggag
     1441 ctcggccggc ctcatcggcc gccccgcgtg cccccggggc cgcccttggc ccccgcgccg
     1501 ccgggccctc cggcgccgcc tggtcccgct gatcggccgc gtcccgactc ggccttggcg
     1561 aggaggccga ggaggccggc ggggccgatc ccgaccaggt tcgggaggac gatgtcggcc
     1621 cgggagagga tgtcggggtc gccgatgccc accgtggcca tgccggcggc gatggccccc
     1681 tcgatgccgg cggccgcgtc ctcgaagacc acacaggccg tgggagggag gccgagctcc
     1741 tcggccgcct tgaggaagac gtcgggggcg ggcttggcgc gctcggcctt gtggccgtcg
     1801 gccaccgcgt cgaaaaggcc agcgatgccc gtggcctcga gtatcgtgga ggtgttcttg
     1861 ctggcgctcg ctatggcagt cctgagcccg gcctccctcg cggcgaggac gaattcccgg
     1921 gcgccgggca ggaccccgct cgggtccatc gtggcgaggt attcgcggta ccaggcgttc
     1981 ttcttgtcgg ccagctccag cttccgctcg gggctggctt cgacccccgc caaggagagg
     2041 atgatgtcga ggctcgccat gcgcgagacg cccttgagcc tctcgttgtc ggccgcgccg
     2101 agctcgccgc cgagctccct cgcgagccgc ctccctgcga ggaaatggaa accggctgtg
     2161 tcgacgatga cgccgtcgag gtcgaagacg aaggccctga tcctgtccat gatcgccact
     2221 tataaacccg ccggcgcggg gggcgcaagg gcgggggctt gggccggggc ccgggcttgg
     2281 gccggggttc cggcttgggc tggggccggc tgtaccaaca agcttcgagg cagcgcctca
     2341 ggccacaagg tgggccttgc ggtaggcggt cggggagagg ccgagggccc tccggaacac
     2401 gcggctgaaa tgggcctggt aggtgaagcc gagcatctgg gcgacctcca tcacgctcag
     2461 gtcggtggcc ctgaggaaat aggtggcctc ctcgaccttc atcttgtgca cgaagtccac
     2521 gaaggagagg cccatcttct tcctgaactg ggtcgagagg tagggggcgc tcaccccgag
     2581 ggcggcggcg gtctcggcca gggtcacctt cttctcgatc ctggcgcgga cgatcctcgc
     2641 ggcgcgcgag accaggacgt cgcgcttctc ggtccggtag cggaggacga gctcgatgaa
     2701 gcgctcgacc atgccctcct cgaagtgggc gagctcctcc tcgccgcggg cggactcgat
     2761 ccgacggatg aaggtgtcgc tcagcgtgaa ggcgagctcg gagtcgaccc cgccctcgat
     2821 ggcggcccgg gtgtagatgg tgcaggagcc gatgatgccg ttcttgaggg aacgcagcct
     2881 gtcggaggcc agggtcgccc tttcccgggc gttgatctcg gcgagggtgg cgagggcggc
     2941 ctcgcggtcc cccgcgacga tgtagtcgct caggcgccgc tcgatgagga agggggcgtg
     3001 gacgaagcgg cggtcgatgt tccgcagccg ctcgcgggcg aaggcccttt cgatgatcga
     3061 ggcgatggac gggtcgctca ttgacgacat tttagcttaa aatcgtgcta aagggaagaa
     3121 gatcgtataa acgggggcgc cggcccggtt taggatagat gcgcaatcgc attatccaaa
     3181 ggaggctccc atcatgcaga aaagtctcgt agcgatactg gccatcgccg ccctgctcgt
     3241 cgcgaccgtg gcggtctcag ccgacccgct ctcggacgcc ttcgcgggca agttcaaggg
     3301 caccgtggtc aggatgtccg gccccttcac cgacaacgac gcggtgaagt tcaacgactc
     3361 gatgaagagc ttcgaggcca ggacggggat cgacgtccag tacgagggct cgaaggagtt
     3421 cgaggcctcc atctccatcc gcgtcgacgg cggcaacgcc cccgacatcg tggacttccc
     3481 gcagcccgga ctcctggaga gcttcgtgaa gaagggcaag gtcatcgacc tgaacaaagt
     3541 tctcgacatg aacaaggtca aggccaacta cagccagagc tggctcgaca tggccaccat
     3601 gaccgggccc aagggcaaga tcgtggccgg ggtctggggc cgggtcaacg gcaagagctg
     3661 cgtctggtac aacaagaagg ccttcgacgc ggcgggctac aaggtcccga ccacctgggc
     3721 cgagctcgtg aagctctgcg accagatcaa gaaggacggg gacacgccct gggccatcgg
     3781 catcgagtcc ggcgccgcca cgggctgggc cgccaccgac tggatcgagg acatcatgct
     3841 ccgcacgacg agcctcgcca actacgacaa gtgggtgaag ggcgagctca agttcgacag
     3901 ccccgaggtc aagaaggcca tgcagaccat ggcggacatg tggttcaccg acggctacgt
     3961 ctatggcggc cgcaaggcca tcgcgacgac cttcttcggc gacgcgccca agcccatgtt
     4021 cgacaacccg cccaaggcct ggctccacag gcagggcaac ttcatcacct ccttcttccc
     4081 gaacaacctc gtcgcgggcc aggactacga cttcttctac ctgccgccga tcgaccccaa
     4141 gtacggcaag cccgtcctcg tcgccggcga catctacgcc atgttcaacg accgccccga
     4201 ggtccgcgcc ctcatgcagt acttctccac gggcgcctcg gtcgagacct gggtcaaggc
     4261 cggcggggcg atctcccccc acaaggactc caagctcgac tggtacacga actacgtcga
     4321 ccgcaaggtc gccgagatga tcctgaacgc gagctcggtc cgcttcgacg gctccgacca
     4381 gatgccgggt gccgtgggcg ccggctcctt ctggaagcac atgacggcat gggtgagcgg
     4441 ctccgagacc ctcgaccagg ccgccaaggc catcgacgcc tcctggccca agtaagcggc
     4501 cccagttttg at
//
LOCUS       DASSQV010000126         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1025080, whole genome shotgun sequence.
ACCESSION   DASSQV010000126 DASSQV010000000
VERSION     DASSQV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025080"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFL04_02200"
     CDS             <1..555
                     /locus_tag="VFL04_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838543.1"
                     /translation="AELARLRRWKAPPPIDRELAMAALKRRALPGGKEIGRLVVKAVE
                     DYGGQFALVLDPLSDVMVFEDRPRLLAYLEENIGKEETVRVLDRFERPLPCGYSDVIL
                     NLGLPNGFVSELRLVTRPIYELWDSHEAIAAEVDSIVAQARAGSRDLSTAEAARVLAL
                     LGELRALYAAAKGEGSETRSTRPA"
     gene            complement(574..1134)
                     /locus_tag="VFL04_02205"
     CDS             complement(574..1134)
                     /locus_tag="VFL04_02205"
                     /inference="COORDINATES: protein motif:HMM:NF045043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838544.1"
                     /translation="MRQLVRCKACGYIMEADKLSDRCPACGVPAKQFEPYDERISDKR
                     RLVLDLHIHPIVVHLPQAFAAFLVPLAAALVMALGDFRALVLDTTRVLTVVLPFSVLA
                     AFAAGLIDGKTRFRRVTTPVLVKKIIIASVFLASSFGAAAIAVFSGLDTASTLGPFAA
                     LCLVDFSAAVVLGYLGFPLIQARFPG"
     gene            complement(1131..1475)
                     /locus_tag="VFL04_02210"
     CDS             complement(1131..1475)
                     /locus_tag="VFL04_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838545.1"
                     /translation="MKYSERIQELMNKGIAASKEALAKAGGQAQTWGEMGLLRIEIVQ
                     KRSQAEKLVAQLGAEAYACFGERGEPSLWAEAPKVHALVARIQELEKSVDGLEERYRK
                     LGGKDSDLEEEA"
     gene            complement(1537..1962)
                     /locus_tag="VFL04_02215"
     CDS             complement(1537..1962)
                     /locus_tag="VFL04_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838546.1"
                     /translation="MNQAPLASSIVALGLLPVAVLYVHTFASGFRKTAAHRVTGVLAV
                     SGDLVLSIFYMLFRTLGGQVQGSTFHPTGMVFAFFVFHGSIALIVILLELWIIVSAIN
                     YHRTKTMFAHHRWLSKLTFVLWFVTFLSGEGVYITSYLR"
BASE COUNT          365 a          677 c          757 g          335 t
ORIGIN      
        1 gccgagctcg cgaggctcag gcgctggaag gccccgcccc cgatcgaccg cgagctcgcg
       61 atggccgccc tcaagcgccg cgccctcccg gggggcaagg agatcgggcg cctcgttgtg
      121 aaggcggttg aggactatgg cggccagttc gccctcgtcc tcgaccccct ctccgacgtg
      181 atggtcttcg aggaccggcc gaggctcctc gcctaccttg aggagaacat cggcaaggag
      241 gagacggtca gggtgctgga ccgctttgag cgacccctgc cctgcggcta ctccgacgtc
      301 atcctgaacc tcggcctgcc caacggcttc gtgagcgagc tccgcctcgt cacgaggccc
      361 atctacgagc tctgggacag ccacgaggcc atcgccgccg aggtcgactc gatcgtggcc
      421 caggccaggg cgggctcccg ggacctgtcc acggccgagg ccgcgcgggt cctcgccctc
      481 ctcggggagc tgcgggccct ctacgccgcg gccaagggcg agggttccga gacgaggtcg
      541 acgaggccgg cctgaggccg gcctgaggca ggcctagccg gggaacctcg cctggatcag
      601 ggggaagccc agatagccca ggacgaccgc ggcgctgaag tccacgagac agagggcggc
      661 gaaggggccc agggtcgacg cggtgtcgag gccgctgaag accgcgatgg ccgctgcccc
      721 gaaggatgag gcgaggaaga ccgaggctat gatgatcttc ttcacgagga cgggcgtcgt
      781 gacccggcgg aaacgggtct tgccgtcgat gaggccagcg gcgaaggcgg ccagcaccga
      841 gaagggcagg accacggtga gcacccttgt agtgtcgagg acgagggcgc ggaagtcccc
      901 gagggccatg accagggcgg ccgcgagggg gacgaggaag gcggcgaagg cctggggcag
      961 gtggacgacg atggggtgga tgtggaggtc gaggacgagg cggcgcttgt cggagatcct
     1021 ctcgtcgtag ggctcgaact gcttggcggg caccccgcag gcggggcatc tgtcgctgag
     1081 cttgtcggcc tccatgatgt agccacaggc cttgcaccgg acgagctgcc tcatgcctcc
     1141 tcctcgaggt cggagtcctt gcccccgagc ttgcggtaac gctcctcgag cccgtcgacg
     1201 ctcttctcga gctcctggat cctcgcgacg agggcgtgga ccttcggggc ctcggcccag
     1261 aggctcggct cgccgcgctc cccgaagcag gcgtaggcct cggccccgag ctgggccacc
     1321 agcttctcgg cctggctccg cttctggacg atctcgatcc tcaagaggcc catctcgccc
     1381 caggtctggg cctggccgcc ggccttggcg agggcctcct tcgaggccgc gatacccttg
     1441 ttcatgagct cctggatccg ttccgagtac ttcatccgaa ggcctcctgt cagtgcccca
     1501 ttctcgcaca tcgccccggt cggggcaaag gcccgtctag cggaggtagc tggtgatgta
     1561 gaccccctcg ccggagagga aggtgacgaa ccagagcaca aaggtcagct tggacagcca
     1621 gcggtggtgg gcgaacatcg tcttcgtccg gtggtagttg atcgcggaga cgatgatcca
     1681 gagctcgagg aggatgacga tgagggctat cgacccgtgg aagacgaaga aggcgaagac
     1741 catacccgtc gggtggaagg tcgagccctg gacctggccc ccgagggtcc ggaacagcat
     1801 gtagaagatc gagagcacca ggtccccgct caccgcgagc acacccgtga cgcggtgggc
     1861 ggcggtcttc ctgaagccgc tggcgaaggt gtggacatag aggacggcca cggggaggag
     1921 gccgagggcc acgatgctgc tcgcgagggg tgcctggttc atggtttctc cttggtgcgg
     1981 cgccgattcg cgcctcgagc tacttttcgg aattcctcgg ccggcgcttg acaggtccgt
     2041 gatggcccac tatatcctgt agccagagaa taaagcaccc ccagctattg tagggtatgt
     2101 atgcaaattc ggtaccaaat gggtgataca tgcg
//
LOCUS       DASSQV010000127         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1149885, whole genome shotgun sequence.
ACCESSION   DASSQV010000127 DASSQV010000000
VERSION     DASSQV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149885"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1175
                     /locus_tag="VFL04_02220"
     CDS             123..1175
                     /locus_tag="VFL04_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838547.1"
                     /translation="MRLVAEGAHALHLMPEASLEDRLLVGCIARPLSLGRVWSFDLER
                     DTARRAFASGLDAATLEARLGLLAGRSLPQSLAFSLKAWEEEYRSLRLYRGLFLVADE
                     RMRPVIERGAALGSLVAELVAPGVYFLATASPEEVGAVLGRSGLEAPPPVAPPIPSFP
                     QALESEAGSGEGAAQGRREGSSGILAELSRMAGFSADGESGAGRGSAVLDPGPRLEEL
                     RAALDRLAGERSPESRRELADRIERRLVLSERQIAESDPRPERLEATGLDYMGKVRVV
                     ERALRSRGDRLEVLYRLPGEEPVRALLRPVKLEKNDKGLVLEAEDLATGGPARVPLGA
                     VSTVRRVRASLFGDEA"
     gene            1172..1960
                     /locus_tag="VFL04_02225"
     CDS             1172..1960
                     /locus_tag="VFL04_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET7838548.1"
                     /translation="MKNEQSQMGQLGHQAQPKLAVLVDADNTQASIIEGLLAEIAKYG
                     VASVKRIYGDWTNTNLRSWKDKLLEFAIQPMQQFSFTTGKNSTDSAMIIDAMDLLYTE
                     SLDGFCIVSSDSDFTRLAVRLREDGKLVLGFGQRKTPKPFVAACDKFIYTEILREPEE
                     EREGRGDRESAPRGSVDMKGDRRMASLLTSAVEDAADEFGWAYLGAVGTYIANRLPDF
                     DPRNYGYRKLGDLIKSSGLFEIDERASPTDQGKQIYIRIKAKGR"
     gene            1968..2516
                     /locus_tag="VFL04_02230"
     CDS             1968..2516
                     /locus_tag="VFL04_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7838549.1"
                     /translation="MLEYRRAQSSDAPVLAELRIEFMRIVKDGGIAGEAELRAELRDR
                     FAAELSAETMVAWICLDAGLVVAASGLGLPAVAAGTRVAGGASGASGASEAGADGEAE
                     ALVFNMYTRASHRRRGIASELLRLCLEEGRARGVLRFRLQSTDDGRGLYERAGFVDEG
                     GDMVLRIQGRAPARQPGAGLVP"
     gene            2575..>2992
                     /locus_tag="VFL04_02235"
     CDS             2575..>2992
                     /locus_tag="VFL04_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HET7838550.1"
                     /translation="MGAAALLGAGALLASCGGEAKAPKPQGRTIVATYSLLGSVVREL
                     AGDAFEVKVSIPNGLDPHEWEPSAKDIQLLQGADLIVENGLGLEEGMSKALDAARAKG
                     LRFFTASDHVTVRRVGAGEGIPSGDRDQAVGARDPHL"
BASE COUNT          454 a         1074 c         1055 g          409 t
ORIGIN      
        1 agccgagcgc ctctgagccg agggtacccg aggcggcagg gacagctggg ccgagggtac
       61 ccgaggcggc agggacagct gagccgaggg tacccgaggc ggcagggaca gctgagccga
      121 gggtgaggct cgtggccgag ggggcccacg ccctccacct catgcccgag gcctcgctcg
      181 aggacaggct cctggtcggc tgcatcgccc gacccctgtc cctcggccgg gtctggagct
      241 tcgacctgga gcgggacacg gcccgcaggg ccttcgcctc gggcctggac gccgccaccc
      301 ttgaggccag gcttgggctc ttggcggggc ggagccttcc gcagagcctg gccttctcgc
      361 ttaaggcctg ggaggaggaa taccgctcgc tccgcctcta ccgtggcctc ttcctcgtcg
      421 ccgacgagag gatgcggccc gtcatcgagc ggggagcggc actgggctcc ctggtcgccg
      481 agctcgtcgc cccgggggtc tatttcctcg cgacggcgag ccccgaggag gtcggggctg
      541 tcctgggccg ctcgggcctc gaggccccgc cccccgtggc gccgccgatc ccctccttcc
      601 cccaggccct cgagtccgag gctgggagcg gcgagggcgc ggcgcagggc cggcgcgagg
      661 ggagctcggg catcctggcc gagctctcgc gcatggcggg tttttccgcc gatggggagt
      721 caggcgccgg ccgcggctcg gccgtcctcg atccgggccc caggctcgag gagctcaggg
      781 ccgcgcttga caggctcgcc ggcgagcgga gccccgaatc caggcgcgag ctcgccgaca
      841 ggatcgagcg caggctcgtc ctgagcgagc ggcagatcgc cgagtccgac cccaggcccg
      901 agcggctcga ggccaccggt ctcgattaca tgggcaaggt gcgcgtcgtc gagcgcgcgc
      961 tccgcagccg aggcgaccgc ctcgaggtcc tctaccgcct ccccggcgag gagccggtcc
     1021 gcgccctcct tcggccggtc aagctcgaaa agaacgacaa ggggctcgtc ctcgaggccg
     1081 aagacctcgc gacgggcggc cccgcccgcg taccgctcgg cgccgtcagc acggtgcggc
     1141 gcgtgcgcgc atccttattc ggagatgaag catgaagaat gaacagagcc agatgggcca
     1201 actcggccac caggcccagc ccaagctcgc cgtcctcgtc gacgccgaca acacccaggc
     1261 ctcgatcatc gagggcctcc tcgctgagat cgccaagtac ggcgtcgcca gcgtcaagcg
     1321 gatctacggc gactggacca acacgaacct ccgcagctgg aaggacaagc tcctcgagtt
     1381 cgccatccag cccatgcagc agttcagttt caccacgggg aagaactcca ccgacagcgc
     1441 gatgatcatc gacgcgatgg acctcctcta caccgagagc ctcgacggct tctgcatcgt
     1501 gtcgagcgac tcggacttca cgaggctggc cgtgaggctg cgcgaggacg gcaagctcgt
     1561 cctcggcttc ggacagcgca agacccccaa gcccttcgtc gcggcctgcg acaagttcat
     1621 ctacaccgag atcctccgcg agcccgagga ggagcgcgag ggcaggggcg accgggagtc
     1681 ggcgccgcgg ggatccgtgg acatgaaggg cgaccgccgc atggcgagcc tcctcacgag
     1741 cgcggtcgag gatgccgccg acgagttcgg ctgggcctac ctcggggctg tcgggaccta
     1801 catagcgaac cgcctgcccg atttcgaccc ccgcaactac ggctaccgca agctcggaga
     1861 cctcatcaag tcctcggggc ttttcgagat cgacgagcgg gcgagcccca ccgaccaggg
     1921 caagcagatc tacatcagga tcaaggccaa gggacgttag ccggactatg ctcgagtacc
     1981 gccgcgccca gagctcggac gccccagtcc ttgccgagct caggatcgag ttcatgcgca
     2041 tcgtcaagga cggaggcatc gccggcgagg ccgaactgcg ggccgagctc cgcgaccgct
     2101 tcgccgccga gctctcagcg gagaccatgg tcgcctggat ctgcctcgat gcgggcctcg
     2161 tcgtggcggc ctcgggcctg ggcttgccgg cggtggctgc cgggactcgc gtggcgggcg
     2221 gggcctccgg ggcctccggg gcctccgagg cgggcgccga tggcgaggcc gaggccctcg
     2281 tcttcaacat gtacacgagg gcctcccaca ggcggagggg catcgcttcc gagctcctcc
     2341 gcctctgcct cgaggagggc cgagcccggg gcgtgctgcg gttcaggctc cagagcaccg
     2401 acgacggccg gggcctctac gagcgcgccg gcttcgtgga cgagggcggg gacatggtgc
     2461 tacggatcca gggccgggcg cccgcgcgcc agccgggagc gggtcttgtc ccctaggcac
     2521 caaggggccg aagctgggcc ttcgcgggcc gcggcactct gcgcagcggg gcttatgggc
     2581 gccgccgccc tgcttggtgc aggggccctc ctcgcgtcct gcggcggcga ggcgaaggcc
     2641 cccaagcccc agggccggac tatcgtcgcc acctactccc tcctcggctc cgtcgtgagg
     2701 gagctcgcgg gcgatgcctt cgaggtcaag gtctcgatcc ccaacggcct cgacccccac
     2761 gaatgggagc cctcggccaa ggacatccag ctcctccagg gggccgacct catcgtcgag
     2821 aacgggcttg gcctcgagga gggcatgtcg aaggccctgg acgccgcgcg cgcgaagggc
     2881 ctcaggttct tcacagcttc cgaccacgtc accgtccgca gggtcggtgc cggcgagggc
     2941 atcccctcgg gcgacaggga ccaggccgtc ggggcccggg acccccatct ct
//
LOCUS       DASSQV010000128         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_777920, whole genome shotgun sequence.
ACCESSION   DASSQV010000128 DASSQV010000000
VERSION     DASSQV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777920"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFL04_02240"
     CDS             complement(<1..279)
                     /locus_tag="VFL04_02240"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET7838551.1"
                     /translation="MKELVHEEIAQSYDLAAPIEKVFAAITSDIGKWWTHRFREGSTV
                     GFEPKIGGRFYEEWAGGSAALYATVTYIEPPARLRLSGPMGMDGAVTST"
     gene            complement(358..651)
                     /locus_tag="VFL04_02245"
     CDS             complement(358..651)
                     /locus_tag="VFL04_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838552.1"
                     /translation="MVYLIRHVRVERDWEKMEDASKAMMDYWRKQPTVKGVEMWGNIA
                     GPQDEFRIVAKFDTLADEEKFALGLWDDKGYGEVFMKFAAVFRLEEDELVRSF"
     gene            836..2158
                     /gene="ffh"
                     /locus_tag="VFL04_02250"
     CDS             836..2158
                     /gene="ffh"
                     /locus_tag="VFL04_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET7838553.1"
                     /translation="MLEKISEKLSDIARTLGGKASITEKNVEEAVEQIKIALLEADVN
                     LRVVRRFVNATLEEAKGEKVLKAVSPGQQFVKIVHDRMVGLLGDTRQDLQLRGPDTQS
                     VILLLGLQGSGKTTTSAKLAFHLKNQGRRVLLVACDLQRPAAIEQLSVLGAQVGVEVY
                     REDSKDAVQVAKAAVAKAKRELFDTVIVDTAGRLQVDEALMAELAQIRDAVKPQESLL
                     VADSMTGQSAADIAKTFDEKIGLTGVVLSKFDSDARGGAALSLKTVTGKPIKFIGTSE
                     KMEGLEPFFPERIAGRILGMGDIVGLVEKAQAVFDQKQAAELERKMADETLTLEDYLE
                     QIASVKKMGSVKNMLEMIPGLSGQIDEDKIDEAGMKREEAILRSMTRKERLNHLIIGP
                     SRRARVAKGSGTSVAEVNRLLKKFEKTRLMMKKMAKNKKYQQQMFGKA"
BASE COUNT          439 a          753 c          677 g          354 t
ORIGIN      
        1 ggtcgaggtc accgccccgt ccatgcccat cgggcccgag aggcgcagcc tcgcgggcgg
       61 ttcgatgtag gtcacggtcg catagagcgc ggccgagccc ccggcccact cctcgtagaa
      121 gcggcccccg atcttgggct cgaagcccac ggtcgaacct tcgcggaacc ggtgcgtcca
      181 ccacttgccg atgtcggacg tgatcgctgc gaagaccttc tcgatcggcg ccgcgaggtc
      241 gtagctctgc gctatctctt cgtgaacgag ctccttcatg ccgcctcccc tcgctggggc
      301 aatactaccg cgaagcgtcg agccgttgaa ccactcgacc gcgacgaagg tccctgccta
      361 gaaggaacgc acgagctcgt cttcctccag gcggaacacg gcggcgaatt tcatgaagac
      421 ctcgccgtag cccttgtcgt cccacagccc gagggcgaac ttctcctcgt cagcgagcgt
      481 gtcgaatttc gcgacgatgc ggaattcgtc ctgggggccc gcgatgttcc cccacatttc
      541 cactcccttg acggttggct gctttcgcca atagtccatc atcgccttgg atgcgtcttc
      601 catcttttcc cagtcgcgct cgacgcgcac gtgtctgatc agatagacca tacctgctcc
      661 ttcttgtata gaataactgt aatataacga gacttgcatt tcgctcaaag ccgctcggct
      721 gtcatgaaga cggcacccgg gccgcgaatg gatgggcgcg tgggcgaacg ggcggcctac
      781 cgggaaagcg acgggtcttg gcaaggccgc ggcttgcggg ggataatccc ccaccatgct
      841 cgagaaaatc agcgagaagc tcagcgacat agccaggacc ctcggaggca aggcctccat
      901 aaccgagaag aacgtcgagg aggcggtcga gcagatcaag atcgccctcc tcgaggccga
      961 cgtgaacctc cgcgtcgtgc ggcgattcgt caatgccacc ctcgaagagg ccaagggcga
     1021 gaaggtcctc aaggccgtga gcccgggtca gcagttcgtc aaaatcgtcc acgaccggat
     1081 ggtgggcctc ctcggtgata cgcgccaaga cctccagctc cgtggacctg atacccagag
     1141 cgtcatcctc ctcctcggcc tccagggctc gggcaagacc acgacctcgg ccaaactcgc
     1201 cttccacctg aagaaccagg gccgcagggt cctcctcgtg gcctgcgacc tccagcgccc
     1261 cgcggccatc gagcagctct ccgtcctcgg cgcccaggtc ggggtcgagg tatacaggga
     1321 ggactccaag gacgcggtcc aggtcgccaa ggcggctgtg gccaaggcca aacgcgagct
     1381 cttcgacacg gtgatcgtcg acacggcggg ccggctccag gtcgacgagg ccctcatggc
     1441 cgagctggcc cagatcaggg acgcggttaa gccccaggag tccctcctcg tcgccgactc
     1501 catgaccggc cagtcggccg ccgacatcgc caagaccttc gacgagaaga tcggcctcac
     1561 gggcgtggtc ctctccaagt tcgactccga cgccaggggc ggagccgccc tctcgctcaa
     1621 gaccgtcacc ggcaagccca tcaagttcat cggcacgagc gagaagatgg agggcctcga
     1681 gcccttcttc cccgagcgca tcgccggccg catcctcggc atgggcgaca tcgtcggcct
     1741 cgtcgagaag gcccaggccg tcttcgacca gaaacaggcg gccgagctcg agcgcaagat
     1801 ggccgacgag accctgaccc tcgaggacta ccttgagcag atcgcctcgg tgaagaagat
     1861 gggctcggtc aagaacatgc tcgagatgat ccccgggctc tcgggccaga tcgacgagga
     1921 caagatcgac gaggcgggca tgaaacgcga ggaggccatc ctccgctcaa tgacgaggaa
     1981 ggagaggctg aaccacctca tcatcggccc cagccgccgc gccagggtgg ccaagggctc
     2041 gggcacctcg gtggccgagg tgaaccgcct ccttaagaag ttcgagaaga cccgcctcat
     2101 gatgaagaag atggcgaaga acaagaagta tcagcagcag atgttcggga aggcctaggc
     2161 gcgaacccgg cccccccccg ccctctccga gggccttcgc atcggggcga acccggtcca
     2221 gtg
//
LOCUS       DASSQV010000129         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1523759, whole genome shotgun sequence.
ACCESSION   DASSQV010000129 DASSQV010000000
VERSION     DASSQV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523759"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VFL04_02255"
     CDS             <1..1075
                     /locus_tag="VFL04_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HET7838554.1"
                     /translation="GGLIYEGLAGNRQEAEALAASGEIAFSPCHHHAAVGPMAGVMTA
                     SMPVWIFQNETYGNRSYCTFNEGLGKVLRYGAFSDEVLDRLRWIERELAPMMKKALAL
                     HGPLDMRSIIAQALQMGDEGHNRNRAGTSLIIREFAPSLVRLGEDGEKVAKALSFMHA
                     NDHFFLNLSMPASKCVVDAAAGVEGSTVITTMARNGTDFGIRISGLPERWFTGPAGIV
                     EGLYLPGFSAADAAPDIGDSVITETAGIGGFAMAAAPAIVKFVGGSPEDALAWTRRMY
                     EITLSESEAYRIPILDFRGTPTGIDLRKVVETGILPVINTGIAHREPGIGMVGAGLVK
                     PPVNCFEDALFAFAETYGEGANA"
     gene            1072..1947
                     /locus_tag="VFL04_02260"
     CDS             1072..1947
                     /locus_tag="VFL04_02260"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7838555.1"
                     /translation="MKMIDLTIPLGIGTPPWPTYIPLQVQYFKRLAPNGANGQVVTHS
                     NHVGTHLDGEIHFYTPGKDIASLDMDFLVHEGAIVDLSDVCKDYDVYTSKMVEERVEV
                     KTGDILIIHTGFHHYGWDQPTADEVRYMIKHPGPDREFAEWAKKKKLRWIGVDCGSAD
                     HPMNTKIREWMPVQAKECDEHFRKKYKKSLEELFTPDKYQVMHIEMFDKGLIHAECVG
                     GDLDLLLNQRATIGCFPWRFVDGESSIARIVAMVEDKRYDELMAKKKKAKLTKFGDVA
                     GAKNPWLHEEARKVK"
BASE COUNT          407 a          719 c          649 g          298 t
ORIGIN      
        1 cggcggcctc atctacgagg gcctggcggg gaaccgtcag gaggccgaag ccctcgccgc
       61 ctcgggcgag atcgccttct cgccctgcca ccaccacgcc gccgtgggcc cgatggccgg
      121 ggtcatgacg gccagcatgc ccgtctggat cttccagaac gagacctacg gcaaccgctc
      181 ctactgcacc ttcaacgagg gcctgggcaa ggtcctccgc tacggcgcct tctccgacga
      241 ggtcctcgac aggcttcgct ggatcgagcg ggagctcgcc cccatgatga agaaggccct
      301 cgccctccac ggccccctcg acatgcgcag catcatcgcc caggccctcc agatgggcga
      361 cgagggccac aaccgcaacc gcgcggggac ctcgctcatc atcagggagt tcgccccctc
      421 cctcgtccgt ctcggcgagg acggggagaa ggtcgccaag gccctctcct tcatgcacgc
      481 gaacgaccac ttcttcctca acctctccat gcccgcctcc aagtgcgtgg tcgacgccgc
      541 ggccggggtc gagggcagca ccgtgatcac gacgatggcg cgcaacggca ccgacttcgg
      601 gatccggatc tcggggcttc cggagcgctg gttcaccggg ccggcgggca tcgtggaggg
      661 cctctacctc cccggcttct cggcggccga cgcggctccc gacatcgggg actcggtgat
      721 caccgagacc gcgggcatcg gcggcttcgc catggctgcc gccccggcca tcgtgaagtt
      781 cgtcggcggc agccccgagg atgccctggc ctggacccgc aggatgtacg agatcaccct
      841 gtccgagagc gaggcctacc ggatcccgat cctcgacttc cgcggcaccc ccacggggat
      901 cgacctgcgg aaggtggtgg agaccgggat cctccccgtg atcaacaccg gcatcgccca
      961 tcgcgagcct ggcataggca tggtcggggc gggactcgtg aagccccccg tgaactgttt
     1021 cgaggacgca ctcttcgcct tcgcggaaac ctacggagaa ggagcaaacg catgaagatg
     1081 atcgatctga cgatccccct cgggatcggg acccctccct ggccgaccta catccccctg
     1141 caggtccagt acttcaagcg cctcgcgccc aacggcgcca acggccaggt cgtgacccac
     1201 tcgaaccacg tcggcaccca cctcgacggc gagatccact tctacactcc cggcaaggac
     1261 atcgcgagcc tcgacatgga cttcctcgtc cacgaggggg ccatcgtcga cctctccgac
     1321 gtgtgcaagg actatgacgt ctacacctcc aagatggtcg aggagagggt cgaggtgaag
     1381 accggcgaca tcctcatcat ccacaccggc ttccaccact acggctggga ccagcccacg
     1441 gccgacgagg tccgctacat gatcaagcat ccggggccgg accgcgagtt cgccgagtgg
     1501 gccaagaaga agaagctgcg ctggatcggc gtcgactgcg gcagcgccga ccaccccatg
     1561 aacaccaaga tccgcgagtg gatgcccgtg caggcgaagg agtgcgacga gcacttccgg
     1621 aagaagtaca agaagtccct cgaggagctc ttcacccccg acaagtacca ggtcatgcac
     1681 atcgagatgt tcgacaaggg cctcatccac gccgagtgcg tgggcggcga cctcgacctc
     1741 ctcctcaacc agagggcgac catcggctgc ttcccctggc gcttcgtcga cggcgagtcg
     1801 agcatcgcgc gcatcgtggc catggtcgag gacaagcgct acgacgagct catggccaag
     1861 aagaagaagg ccaaactcac gaagttcggc gacgtagccg gcgccaagaa cccctggctc
     1921 cacgaggaag cccgcaaggt caagtagcgc attgaggcag atgaaccgca gaggcgcaga
     1981 gacgcagaga cgcagagacg cagagggagg agattctcgg gggattgtag agaagaggga
     2041 gagaggctgg cgagttggag tgcggcgaag ctc
//
LOCUS       DASSQV010000130         7217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_405994, whole genome shotgun sequence.
ACCESSION   DASSQV010000130 DASSQV010000000
VERSION     DASSQV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405994"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VFL04_02265"
     CDS             complement(<1..1052)
                     /locus_tag="VFL04_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET7838556.1"
                     /translation="MDTLLRINMGAKGGPAISKEPVGAYAGLGGRALTSAIVSKEVPP
                     LCHALGAENKLVIAPGLLSGSPASQSGRISIGCKSPLTGGIKESNAGGQAAQVLARLG
                     YAAIVLEGAPAGNDLYKIVVDKTGVRISVDNGLRMLGNYALIDALKPVHGDKAAYISI
                     GPAGEMRLAAASIACTDMELHPTRHAGRGGVGAVMGSKGVKVIVLDDEGMKTREPKDP
                     EKFRAANKAWVEGLKKHPVTGGGLPSFGTNVLTNIINEAGGFPTNNFSKGQFAGAAKI
                     SGEAQAALETQRGGLATHGCHRGCVIQCSGIYNDAKGNFLSKQPEYETVWAHGANCGI
                     DDLDTIAMLDRLDDDIG"
     gene            complement(1243..3174)
                     /locus_tag="VFL04_02270"
     CDS             complement(1243..3174)
                     /locus_tag="VFL04_02270"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HET7838557.1"
                     /translation="MSVDYIERGFERCRAYGIDPGQVHSSRILGEEELRDRLARKREL
                     MVAAEPFIGHLYDFLRGSGFFSILTDDEGCILSLIGDEGILREASALMMVPGAFMDER
                     SIGTNAMGTALAEGMPVQVSGDEHYIRAYHRWTCSGAPIRDAEGRIIGSLDLTGNSER
                     VHQHTLGMVVAAVNAIEKTLILGSKNEALKSTKRFVETLIDSIQAGIVSVNLEGEILS
                     VNSWALEMFGFGSEEMLGRRVGAFLQNWGEVRMACVSGGDFQNKDVLVDVRANKLYFN
                     LTCYPIVGENAEPTGLILVFKDVKKVRKHANEILGRRALYTFDKILGGSRPIVEAIEF
                     ARKVADCRSTILITGESGTGKEIFAQAIQNASSRADEPFVVLNCGAIPRTLIESELFG
                     YADGAFTGAKRGGQAGKFEIADGGTIFLDEIGEMPLDMQTRLLRVIEEGTVMRVGDSR
                     DIPVDVRIIAATNKDLKAEVAVGNFRMDLFYRLNVLPLRLPPLRECREDIPLLFDFYM
                     NKISKKLNKRPVQVSQEYMGQLMAYEWPGNVRELENMIELLISSERLPSLPGGRRSEP
                     ARAGIARGEPAAMTLEDSERRIIGEALEACLYNISGAAKRLGIGRNTLYRKMERYGIT
                     CPIPEHRPIMEQAPEKAVS"
     gene            complement(3287..3985)
                     /gene="pyrH"
                     /locus_tag="VFL04_02275"
     CDS             complement(3287..3985)
                     /gene="pyrH"
                     /locus_tag="VFL04_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET7838558.1"
                     /translation="MTTVLSLGGSIVAPEGPDAAFLASFLRLVRDYLKAEASRRLIIV
                     VGGGGPARRWQQAYREVLECGAGDEHEAQDWIGIMATRLNAQLVKALLGELCPQEVVT
                     DPTAVGVFMGKVLVAAGWKPGFSTDNDAVLLAERFGADRIFNLSNIAKVYTDDPRTNP
                     EAKPIDAIGWAEFRKLVGEDWSPGKNTPFDPVATRKAAELGLSVVCAAGRDLANLREM
                     LEGRSFVGTLIGPN"
     gene            complement(4017..5126)
                     /locus_tag="VFL04_02280"
     CDS             complement(4017..5126)
                     /locus_tag="VFL04_02280"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HET7838559.1"
                     /translation="MKRHSLSLILLVLASMAWSLETTQIGKVDYIEGGVSINRVGKTL
                     SDPNIDDPLYSGDLIKTAGDGMLVIAMDRSTGMGGTITIRSRAALYINISMVKGQPKT
                     SIDLLAGSIGSKVSKLSGSPTMAVNTNGAVMGVRGTEYGVAVSVNEAVLVTCTEGQVA
                     VTDGGEELPVPAGKALEKRPGEKLRYLPVAVSSVKDYNERWIADEIEAFKADAPRALA
                     DYAKRYNDLSAKFAAAIDPFQKSATVRKWFDEDRTGVVVNPMDPAVLREKKEVAGLLL
                     DLRRVLFLFERVYYRVDDLSGIIAGTPYERTLVSPGVTAGDFLHRVADEREQLSRQVA
                     RFRYAEKLYEARSPEGEVFGSGSGNDDFFKSPDGF"
     gene            complement(5268..6509)
                     /locus_tag="VFL04_02285"
     CDS             complement(5268..6509)
                     /locus_tag="VFL04_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838560.1"
                     /translation="MNRIAQVFVLAALLAQAATAFSQDSGQPSEQDVLQKARDKYQAG
                     TATAPAQSGQAASDQAAEAQAGPADQDHPGSGHPYSPYVLAFVPGIQFPFGVLDTSFS
                     AGWIGALTGDVSGFQAAGVFNLSGRVEGFQGAGVFNISGPVDGFQGAGVFNIARGRID
                     GFQGAGVFNMAETAEVPIQAAGVFNMAKRVGGFQAAGVFNLAEEVDGGQFAGVFNVAR
                     NVKGVQVGLVNVANEVDGVQLGLVNISRNGVDSLAFSYEPETDYVYGHWQAGTRHLYT
                     LAGLGLPRRQWFNGEDGMVLSFGLGSRIALEAAYLDIDVSAESMVGRRLGRFLSADES
                     EQTESAWRAVWDPAYPSIRLSLGLPLSHRVHLIGGIKMDVDLAERPGVPADLKQGKSF
                     SDTWFGHPFTVWPKWFFGVKF"
     gene            6658..>7217
                     /locus_tag="VFL04_02290"
     CDS             6658..>7217
                     /locus_tag="VFL04_02290"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HET7838561.1"
                     /translation="MGLFSAFRAARRRRILAASPIPDELWDWAIREHRIFRWLSTEDL
                     VRLRELSTIFLGEKVFDPVGGLELDEETRVSIAAQACLPLLGLGIDWYNDWSTIIVTP
                     REYGVEKRDVDEAGVVHEYDDEFAGEAFDLGPVALSKVDIEASGWGDGYNVVIHEMAH
                     KLDGRNGAYDGCPPLRSGMDQGAWKRA"
BASE COUNT         1081 a         2307 c         2567 g         1262 t
ORIGIN      
        1 ccgatgtcgt cgtcaaggcg gtcgagcatg gcgatcgtgt cgaggtcgtc gatgccgcag
       61 ttggcgccgt gggcccagac cgtctcgtac tcgggctgtt tggagaggaa gttgcccttg
      121 gcgtcgttgt agatccccga gcactggatg acgcagcccc tgtggcagcc gtgggtcgcg
      181 aggccgccgc gctgggtctc gagggcggcc tgggcctcgc ccgagatctt ggcggctccg
      241 gcgaactggc ccttggagaa gttgttggtg gggaagccgc ccgcctcgtt gatgatgttc
      301 gtgaggacgt tggtgccgaa gctggggagg ccgcccccgg tgacggggtg cttcttgagg
      361 ccctcgaccc aggccttgtt cgccgccctg aacttctcgg ggtccttggg ctcgcgggtc
      421 ttcatgccct cgtcgtcgag gacgatgacc ttgaccccct tggagcccat gacggcgccg
      481 acgccgccgc ggcccgcgtg gcgggtgggg tggagctcca tgtcggtgca ggcgatggag
      541 gcggcggcaa gcctcatctc cccggccggg cctatggaga tgtaggctgc cttgtcgcca
      601 tggaccggct tgagcgcgtc gatgagggcg tagttgccga gcatcctgag gccgttgtcg
      661 acgctgatcc tgaccccggt cttgtcgacg acgatcttgt agaggtcgtt gccggccggg
      721 gcgccctcga ggacgatggc ggcgtagccg agcctggcga ggacctgggc cgcctggccg
      781 ccggcgttcg attccttgat cccgcccgtg agcgggctct tgcagccgat ggagatgcgg
      841 ccggactggg aggccggact gccggagagg agaccgggcg ctatgacgag cttgttctcg
      901 gctccgagcg cgtggcagag gggagggact tccttcgaga cgatagcaga agtgagggct
      961 cggccgccga ggcccgcgta ggctccgacc ggctccttag agatcgcggg tccgcccttc
     1021 gcgcccatgt tgattcgcag caaggtatcc atgacagcct cctcggaatg ctatgcggat
     1081 actccgcagg aagcgtgcca gagtagaccg gcttctcggg tcggctcagg cctccggata
     1141 gcgcaggaaa ccgcggaatc tgtcttcata cgcggttcgg cgcgggaggc cagggggcgg
     1201 acttcggctc aggggcgggg acccgggggc gcctgctcca aatcaggaca cagccttctc
     1261 cggggcctgt tccattatgg ggcggtgttc cggaatgggg cacgtgatgc catagcgctc
     1321 catcttgcgg tagagggtgt tccggcctat gccgaggcgt ttggcggcgc cggagatgtt
     1381 gtagaggcag gcctcgaggg cctccccgat gatccggcgc tcagagtcct cgagggtcat
     1441 cgcggccggc tcgccgcgcg cgatgccggc gcgggccggt tcgctcctgc ggccgccggg
     1501 cagggagggc aggcgctcgc tgctgatgag gagctcgatc atgttctcga gctctcgcac
     1561 gttgcccggc cactcatagg ccatgagctg gcccatgtac tcctggctca cctggaccgg
     1621 cctcttgttg agcttcttcg agatcttgtt catgtagaag tcgaagagga gggggatgtc
     1681 ctcccggcat tcgcgaaggg gcggcagcct gaggggaagc acgttgaggc ggtagaagag
     1741 gtccatccgg aagttgccga cggccacctc ggccttgagg tccttgttcg tggcggcgat
     1801 gatgcgcacg tcgacgggga tgtccctcga gtcgcccacc cgcatcaccg tgccctcctc
     1861 gatgacgcgg agcagcctgg tctgcatgtc cagcggcatc tcgccgatct cgtcgaggaa
     1921 aatggtgccc ccgtcggcga tctcgaactt gcccgcctgg cctccccgct tggccccggt
     1981 gaaggcgccg tcggcgtagc cgaagagctc ggactcgatg agggtccgcg ggatggctcc
     2041 gcagttgagc acgacaaagg gctcgtcggc gcgcgagctc gcgttctgga tggcctgggc
     2101 gaagatctcc ttgcccgtcc ccgactcgcc ggtgatgagg atggtggatc tgcagtcggc
     2161 gaccttccgc gcgaactcga tggcctcgac gatgggccga gagcccccga ggatcttgtc
     2221 gaaggtgtag agggcccgcc gtccgaggat ctcgttggcg tgctttcgga ccttcttcac
     2281 gtccttgaag acgagaatga ggccggtcgg ctcggcgttc tcgccgacga tggggtagca
     2341 ggtgaggttg aagtacagct tgttggcgcg gacgtcgacg aggacgtcct tgttctggaa
     2401 gtccccgccc gagacgcagg ccatgcggac ctcgccccag ttctggagga aggcgcccac
     2461 gcggcggccg agcatctcct cgctgccgaa gccgaacatc tcgagggccc agctgttcac
     2521 cgagaggatc tcgccctcga ggttgaccga gacgattccg gcctggatgg agtcgatgag
     2581 ggtctcgacg aagcgcttgg tgctcttgag ggcctcgttc ttggagccga ggatgagggt
     2641 cttctcgatc gcgttgaccg ccgcgaccac catgccgagg gtgtgctggt ggacccgctc
     2701 gctgttgccg gtgaggtcga gggagccgat gatgcggccc tcggcgtcgc ggatgggagc
     2761 ccccgaacag gtccagcggt ggtaggccct gatgtagtgc tcgtcgcccg agacctggac
     2821 gggcatgccc tcggccaggg ccgtgcccat ggcgttcgtg ccgatgctcc gctcgtccat
     2881 gaaggcgccg ggcaccatca tgagggccga ggcctccctg aggatgccct cgtcgccgat
     2941 gagggagagg atgcagccct cgtcgtcggt gaggatcgag aagaagccgc tgcccctgag
     3001 gaagtcgtag aggtggccga tgaagggctc ggcggcgacc atgagctcgc gcttccgcgc
     3061 gaggcgatcg cgcagctcct cctcgccgag aatgcggctg gaatggacct ggccgggatc
     3121 gatcccgtag gcgcggcagc gctcgaagcc gcgctcgatg tagtccacgc tcatggactc
     3181 cctccacgct cttgggtggc agacagccca tgatacgccc gttccgcggt tctggaaacg
     3241 ggggtcaggg cttccgggcc cgggagccgg gccgcggccc tcggcctcag ttgggcccga
     3301 tcagggtgcc gacgaagctc ctgccctcca gcatctcacg gaggttggcg aggtcccgac
     3361 ccgccgcgca cacgacgctg aggcccagct cggcggcctt cctcgtcgcg acgggatcga
     3421 agggagtgtt cttgcccggg ctccagtcct cgcccaccag cttgcggaac tccgcccagc
     3481 ctatcgcgtc gatcggcttg gcctcggggt tggtcctggg gtcgtcggtg tagaccttgg
     3541 cgatgttgga gaggttgaag atgcggtcgg ccccgaagcg ctcggcgagg aggacggcgt
     3601 cgttgtccgt cgagaagccg ggcttccagc ccgcggccac gaggaccttg cccatgaaga
     3661 cgccgacggc tgtcgggtcg gtgacgacct cctggggaca gagctcgccg aggagggcct
     3721 tcacgagctg ggcgttgagc ctggtggcca tgatcccgat ccagtcctgg gcctcgtgct
     3781 cgtccccggc tccgcactcg aggacctcgc ggtaggcctg ctgccagcgc cgcgccgggc
     3841 cgccgccccc gaccacgatg atgagccgcc tcgaggcctc ggccttgagg tagtcccgca
     3901 cgagccgcag gaaggaggcg aggaaagcgg catcggggcc ttcgggcgcg actatggagc
     3961 cgccgagcga gagcactgtc gtcatcgtgg gaacctccgc ggtctgggcc tgactcctag
     4021 aagccgtccg gggatttgaa gaagtcatcg ttccccgaac cggagccgaa gacctcgccc
     4081 tcgggactcc tggcctcgta cagtttttcg gcgtagcgga accttgccac ctgccgcgaa
     4141 agctgctcgc gctcgtccgc gacccggtgc aggaaatccc ccgccgtcac gcccggggac
     4201 acgagggtcc tctcgtaggg cgtgcccgcg atgataccgg aaaggtcgtc gacgcgatag
     4261 tagacgcgct cgaagaggaa gaggacgcgg cgcaggtcga ggaggaggcc ggccacctcc
     4321 ttcttctcgc ggaggaccgc ggggtccatg ggattgacca cgacgcccgt gcggtcctcg
     4381 tcgaaccact tcctgaccgt ggcggacttc tggaaggggt cgatcgctgc ggcgaacttc
     4441 gccgacaggt cgttgtagcg cttcgcgtag tcggcgaggg cccgcggggc gtcggccttg
     4501 aacgcctcga tctcgtcggc gatccagcgc tcgttgtagt ccttcaccga ggagaccgcg
     4561 acggggagat agcggagctt ctcgcccggg cgcttctcga gggccttgcc cgcgggcacg
     4621 ggaagctcct cgcccccgtc ggtgaccgcg acctggccct cggtgcaggt gacgaggacg
     4681 gcctcgttga ccgagacggc caccccgtac tcggtgccgc gcacgcccat gacggccccg
     4741 ttcgtgttga cggccatggt cgggctgccc gagagcttcg agaccttgga gccgatcgag
     4801 cccgcgagca ggtctatgct cgtcttgggt tggcccttga ccatcgagat gttgatgtag
     4861 agcgcggccc tcgagcggat cgtgatcgtc ccgcccatgc ccgtgctcct gtccatggct
     4921 atgaccagca tgccgtcgcc ggccgtcttg atgaggtcgc ccgagtaaag cggatcgtct
     4981 atgttgggat cgctcagggt cttgccgacg cggttgatcg agaccccgcc ctcgatgtag
     5041 tcgaccttgc cgatctgggt agtctcgagg gaccaggcca tggacgcgag gacgagcagg
     5101 ataaggctga ggctatgtcg tttcatgatg gctccttgtg agcggcccgc ggccttcaga
     5161 cgccggcggc ggaaggtcct ggccttccac ccagtataac gcgcccccgg ggggctttcg
     5221 agccgccgga aaggcggtct cgagcccgag ggcgccccct gcatgcccta gaacttgacg
     5281 ccgaagaacc acttgggcca gacggtgaag ggatggccga accaggtgtc gctgaaggat
     5341 ttcccctgct tgaggtcggc ggggactccg gggcgctcgg cgaggtcgac gtccatcttt
     5401 atgcccccga tgaggtggac gcggtggctc agtggcaggc cgaggctgag cctgatggag
     5461 ggataggcgg gatcccagac ggcgcgccac gcggattcgg tctgctcgct ctcgtcggcg
     5521 ctgaggaagc ggcccagcct ccgcccgacc atgctctcgg ccgagacgtc gatgtcgaga
     5581 taggcggcct cgagggcgat ccggctgccg agcccgaagc tcaggaccat gccgtcctcg
     5641 ccgttgaacc actgccgcct gggcaggccg aggccggcga gggtgtagag gtgccgggtc
     5701 ccggcctgcc agtgcccata gacgtagtcg gtctcgggct cgtagctgaa ggcgaggctg
     5761 tcgaccccgt tgcgcgagat gttgacgagg ccgagctgga ccccgtcgac ctcgttggcg
     5821 acgttgacga ggcctacctg gacgcccttc acgttcctgg cgacgttgaa gacgccggcg
     5881 aattggccgc cgtcgacctc ctcggcgagg ttgaacaccc ccgccgcctg gaagcccccg
     5941 acgcgtttcg ccatgttgaa gaccccggcg gcctggatgg ggacctcggc cgtctcggcc
     6001 atgttgaaga ccccggctcc ctggaagccg tcgatccttc ccctcgcgat gttgaagacc
     6061 ccggccccct ggaagccgtc gacggggccc gagatgttga acaccccggc cccctggaag
     6121 ccctctaccc tgccggagag gttgaatacg cccgcggcct ggaagcccga gacgtctccc
     6181 gtcagggccc cgatccagcc ggcgctgaag gaggtgtcga ggacgccgaa gggaaactgg
     6241 atgccgggga cgaaggcgag gacgtagggc gagtaggggt ggccggatcc gggatggtcc
     6301 tggtcggcgg ggccggcctg ggcctctgcg gcttggtcgg acgcggcctg gccagactgg
     6361 gctggcgctg tggcggtgcc ggcctggtac ttgtccctgg ccttctggag aacatcctgt
     6421 tctgaaggct ggcctgaatc ctggctgaag gcggtggcgg cctgcgcgag cagcgccgct
     6481 aggacgaaaa cctgagcgat gcggttcatg atctccccct ggggcgagtt gggctccgtg
     6541 ccccgctgcg gggcgcacgt gccgtcaaaa taccgcgata accgcgaaaa tcaatgggct
     6601 tgggcgatat tctttgaacg ttccccttgg accctccccc ggggtatcat tggcggcatg
     6661 ggtctgttct cagccttccg cgccgcaagg cgccgcagga tcctcgccgc ctcgccgatc
     6721 cccgacgagc tctgggactg ggcgatccgc gagcaccgca tcttccgctg gctctccacc
     6781 gaggacctgg tcaggctccg cgagctctcg acgatcttcc tcggcgagaa ggtcttcgac
     6841 cccgtggggg gcctggagct cgacgaggag acgagggttt caatagccgc ccaggcctgc
     6901 cttcccctcc ttggcctcgg catcgactgg tacaacgact ggtcgacgat catcgtcacg
     6961 ccgagggagt acggggtcga gaagcgcgac gtcgacgagg ccggggtcgt ccacgagtac
     7021 gacgacgagt tcgccggcga ggccttcgac ctcggccccg tggcgctttc gaaggtcgac
     7081 atcgaggcct cgggctgggg cgacggctac aacgtggtga tccacgagat ggcccacaag
     7141 ctcgacggga ggaacggggc ctacgacggc tgccccccgc tccgctcggg catggaccaa
     7201 ggcgcctgga agcgggc
//
LOCUS       DASSQV010000131         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_157929, whole genome shotgun sequence.
ACCESSION   DASSQV010000131 DASSQV010000000
VERSION     DASSQV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157929"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VFL04_02295"
     CDS             complement(<1..832)
                     /locus_tag="VFL04_02295"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7838562.1"
                     /translation="MSLTTCDIFKVGLGAKVDVASPVVDVSSLKNGDYIRGTKTLSGS
                     ATDDTGVKSVTLIVNNGSVQLASIAATVNAETWTAVLDTNALAPSQEIQADLILRITD
                     DTGKTSDKKLVLYFDNVAPSIASLSPTKENLEKTDTLMSGTVAFSGSIKDGLGVKDLV
                     FTAGGIAFPLKAGTTPASWTVEVNTARNASNQPMFVPGTASGVVDLGGGQLKVPLKID
                     AVDNAGNSTSVQTFFYVDQNAAAPKIDFPSPFNPTILYPNLTPIQASNVLAPKQQISF
                     R"
     gene            complement(1385..>2174)
                     /locus_tag="VFL04_02300"
     CDS             complement(1385..>2174)
                     /locus_tag="VFL04_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7838563.1"
                     /translation="FVAVNISALPESLAESELFGHRRGSYTGAEADRMGRFEEADRGT
                     LFLDEIGDTSAALQIKLLRAIQFGVIERVGENLQRKLDVRIIAATNQDLAGLVAAGRF
                     RRDLFYRIKVIEITLPPLRERKEDIESLVAHFIGGFNERNSRRVRGLSREAFDRLMKH
                     SFPGNVRELENIIEHAVVLCRGDLILERDLPEFGAAAGDAGEAGLQAEQSYEEAMLGF
                     ETALLGRALARAQGNKSKAARGLGISDRHLRSRIQRLGMGQGGG"
BASE COUNT          357 a          670 c          742 g          405 t
ORIGIN      
        1 cccggaagga gatctgctgc ttgggcgcca ggacgttgga ggcctgtatg ggagtgaggt
       61 tcggatagag gatcgtgggg ttgaagggcg aggggaaatc gatcttgggc gccgccgcgt
      121 tctggtcgac gtagaagaag gtctggaccg aggtcgagtt gccggcgttg tccacggcgt
      181 cgatcttgag ggggaccttg agctggccgc cgccaaggtc aacgacgccg ctggccgtcc
      241 cggggacgaa catgggctgg ttcgaggcgt tcctggcggt gttcacctcg acagtccagg
      301 aggcgggggt ggtccctgcc ttgagcggga aggctatgcc tcccgccgtg aacacgaggt
      361 ccttcacccc gaggccgtcc ttgatgctcc cgctgaaggc gacggtcccg ctcatgaggg
      421 tgtcggtctt ctcgaggttc tccttggtag ggctcaggga tgctatggag ggggctacgt
      481 tgtcgaaata gaggacgagc ttcttgtcgc tcgtcttgcc cgtatcgtcg gtgatcctga
      541 ggatcaggtc ggcctggatc tcctggctcg gggcgagggc gttggtgtcg agcaccgcgg
      601 tccaggtttc ggcgttcacc gttgctgcga tggaggccag ctgcaccgag ccgttgttga
      661 cgatgagggt gacggacttg acgcccgtgt cgtccgtggc gctgccgctc agggtcttgg
      721 tgccgcggat gtagtcaccg ttcttgagtg aggagacatc caccacggga gaggcgacat
      781 ccaccttggc gccgaggccg accttgaaga tgtcgcaggt cgtgagagac aaggacgcgg
      841 ccgaaaccgc gcagcaagcg agaatgggca gagctctgcc aaaggcacgc ttcgcttcca
      901 tgtcgacccc ccctgaggtg tgaggactta gggtgcactt ttagggatcg acgtttcggg
      961 cgttagccta gaaggaaatt cgaaggccgc cggggtcgga gaagcgtagt gccgatagct
     1021 atgtatcggt ccggcttctg accttctgca gctttcatta tagatcattt cctccaaagg
     1081 cgcaaaccct tctgcaagcc ccatgccaat ccccccggcc gaaactgccg gagtatctcc
     1141 ttttcctcaa ataaaataaa aattctcgtc cgccacgccc ccgaacccgc ccgcgggatt
     1201 tcgaccgccc ggtcggaagt tcgaccagag agtcgaaccc aatctcgccg catccatccc
     1261 gcagcgcagg gtcgggccgc cgtccggctt gggcatcatc ggggacggaa atttccgatt
     1321 ccggatctcc acgccgtccc tcggcagcct cttgcggccc gcgacagcca ccggtgcctc
     1381 gccttcagcc cccgccctgt cccatgccca gacgctggat ccgcgaccga agatggcgat
     1441 cgctgatgcc gaggccgcgg gcggccttcg acttgttgcc ctgggctcgg gcgagggccc
     1501 gcccgagaag ggcggtctcg aagcccagca tggcctcctc gtaggactgt tcggcctgca
     1561 gcccggcctc gccggcgtcg ccggcggcgg ccccgaactc gggcaggtcg cgctcgagga
     1621 tgaggtcgcc gcggcagagc acgaccgcgt gctcgatgat gttctcgagc tccctcacgt
     1681 tgcccgggaa gctgtgcttc atgagcctgt cgaaggcctc gcgggacagg ccgcggacac
     1741 ggcgcgagtt gcgctcgttg aacccgccga tgaagtgagc cacgaggctc tcgatgtcct
     1801 ccttgcgctc gcgcaggggg ggaagggtga tctcgatcac cttgatccga tagaacaggt
     1861 cgcggcggaa acggccggca gcgacgaggc ccgcgagatc ctggttggtc gcggcgatga
     1921 tcctcacgtc gagcttcctc tggaggttct ccccgacgcg ctcgatcacg ccgaactgga
     1981 tggcccgcag gagctttatc tggagggccg cgctggtgtc tccgatctcg tccaggaaga
     2041 gggtgccgcg gtcggcctcc tcgaagcggc ccatgcggtc ggcctcggcc ccggtgtagg
     2101 agccgcggcg gtgaccgaag agctcgctct cggcgaggga ctcgggcagg gcagagatgt
     2161 tcaccgccac aaag
//
LOCUS       DASSQV010000132         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1399763, whole genome shotgun sequence.
ACCESSION   DASSQV010000132 DASSQV010000000
VERSION     DASSQV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399763"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..581)
                     /locus_tag="VFL04_02305"
     CDS             complement(267..581)
                     /locus_tag="VFL04_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET7838564.1"
                     /translation="MPIYTIAEYRVRPEAVGKVKAAIEEFVHYVKANEPRTRLYAAWQ
                     EEGESTSFVHFFIFEDEAAHKAHGESAAVKRFEAVYAPELVGGPVRFTDYRLVAAQGR
                     LE"
     gene            683..1177
                     /locus_tag="VFL04_02310"
     CDS             683..1177
                     /locus_tag="VFL04_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838565.1"
                     /translation="MAGFDRGAAEAKRFSYQRSPDWPKVEEAHRQREPTCACCKPGMD
                     TGAAVQVHHIFPFHYCIALGRPDLELDERNLITLCEKDDASPSEDHHLLVGHLNLFQS
                     ANLKVVMDAKKTYHGSRERQIKGSPLWEKEVHERLKPLDAMSADEKGALRKLMDKTFP
                     ERTP"
     gene            complement(1249..1941)
                     /locus_tag="VFL04_02315"
     CDS             complement(1249..1941)
                     /locus_tag="VFL04_02315"
                     /inference="COORDINATES: protein motif:HMM:NF021529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2259 domain-containing protein"
                     /protein_id="HET7838566.1"
                     /translation="MKKRILITAFAILVATLPVVAGDVATFVDLGFSSDSAYFMFGQY
                     GADQATGKPYAETYIVDNGRNDFVAQGVARKVYDASLEPGLDGVGAFYTLFADQVPLS
                     KKYRIDHLKPGRLLYVLLDGQEPPATLSFRDFKSGAAYEIALNQKTSEAKGGTSSSFG
                     ISVSVKLGDGATKRVTGGNPDIKRPGVKAYVIRRIIAAPDQKTLVFIVEKRMANGGDG
                     GIRYMVEVLKLP"
BASE COUNT          391 a          700 c          688 g          377 t
ORIGIN      
        1 cgtcagaaca gtggcgcaga gctgcggagg tcgttcgccc ctctccgccg gcagcccacg
       61 ccccgcgtcc gcgcccagcc cgcccaagca tggcgcagga ggatcagtcg agggtaaggc
      121 gacctcctgg gccgcggctc ccccacgctg cggcgtcctc ggcctggcgc atccatcgaa
      181 ttgcgccagg cctccggtcg cctccgctac ccccacacgg cttcagggga ttctcggcct
      241 cgaggggtcc cggggaaggg gactccttac tcgaggcggc cctgcgcggc gaccagcctg
      301 tagtcggtga acctcacggg gccaccgacg agttccggag catagaccgc ctcgaagcgt
      361 ttgacggctg cggattcgcc gtgcgccttg tgggcggctt cgtcctcgaa aatgaagaag
      421 tgcacaaatg aagtggattc gccctcttcc tgccaggcgg catagagcct ggttcgaggc
      481 tcgtttgcct tcacatagtg aacgaactcc tcgatcgcgg ccttgacctt cccgacggcc
      541 tctggcctca ccctgtattc ggcgatggtg tatatcggca tgacctgctc cttcccatat
      601 caataatact ttacggcccg ggaatatcgc tgcatagtga ctccattctc accaaatccg
      661 gttttcgtaa aaggagcggg atatggcagg attcgatcgt ggagcggcgg aggcgaagcg
      721 cttttcgtat cagaggagtc ctgattggcc caaggtcgag gaggctcacc gccaaaggga
      781 gcccacctgc gcctgctgca agccgggcat ggacacgggc gctgcggtcc aggtccacca
      841 catcttccct ttccactact gcatcgccct cgggcggccc gacctcgagc tcgacgaacg
      901 caacctcatc accctctgcg agaaggacga cgcctcgccc agcgaggacc accatctcct
      961 cgtcggccac ctgaacctct tccagtcggc gaacctgaag gtcgtcatgg acgccaagaa
     1021 gacctaccac ggatcgcggg agcggcagat caagggcagc ccactctggg agaaggaggt
     1081 gcacgagcgt ctgaagcccc tcgatgcgat gagcgcagac gaaaagggcg ccctgcggaa
     1141 gctcatggac aagacctttc ccgaacggac tccctagccg cggcgatgcc ctggggaaac
     1201 gaaaagcccg gcctctcggc cgggctccct tgtcctttcg cagccgcctc agggcagctt
     1261 gagcacttcc accatgtacc tgatgccccc gtcgccgccg ttcgccatgc gcttctcgac
     1321 gatgaagacc agggtcttct ggtcgggagc cgcgatgatg cggcggatga cataggcctt
     1381 cacgccgggg cgctttatgt cggggttgcc ccccgtcacg cgcttggtgg cgccgtcgcc
     1441 gagcttgacg ctgacggaga tcccgaagga ggatgaggtc ccgcccttgg cctccgaggt
     1501 cttctggttc agggcgatct cgtaggcagc gcccgacttg aagtcccgaa aagagagggt
     1561 ggcgggcggc tcctggccgt ccaggaggac gtagaggagg cggccgggtt tcaggtggtc
     1621 gatcctgtat ttcttggaga gggggacctg gtcggcgaag agggtataga aggcgcccac
     1681 cccgtcgagc cctggctcga ggctcgcgtc atagaccttc cgcgccacgc cctgggccac
     1741 gaaatcgttc ctgccgttgt cgacgatgta ggtctcggcg taaggcttgc ccgtggcctg
     1801 gtcggctccg tactggccga acatgaagta ggccgaatcg gaagagaagc cgaggtcgac
     1861 gaaggtcgcc acgtctccgg cgactaccgg caatgtggcg acaaggatcg cgaaggccgt
     1921 gatgaggatc cgtttcttca tggcaagctc ctctcctgtt tatcggccgc caagaccccc
     1981 ggcttgaacc ctcactgcgt gttgtcggcc aactcctgat ggatgagggc ggcggcgaga
     2041 ccggcagcga aggttccggc gaggacaaga tagctcccgg ccccaagcag ctcgacgcgg
     2101 gggccggcga ttcccgtcag gaggggcacg atggaccagg ggaaccagcg cgacca
//
LOCUS       DASSQV010000133         2547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_654534, whole genome shotgun sequence.
ACCESSION   DASSQV010000133 DASSQV010000000
VERSION     DASSQV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654534"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /gene="nudC"
                     /locus_tag="VFL04_02320"
     CDS             complement(<1..793)
                     /gene="nudC"
                     /locus_tag="VFL04_02320"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HET7838567.1"
                     /translation="MESTTQIFLFEGLDILIPGDNSATDVPGGGSRADVPGPAVPAGV
                     ADAIVPVLSRRFARGGGRYEARLLDPAGAAAALALGLARVPVRQVIGSFDAEAMRPAL
                     TGLALLHWLSGALRCGACGEGLVDAPLAKPGDPAGHVSAEPEYREGGGSRVCPVCGRE
                     HFPRISPAVIVLVRQGQEALLARNARFPPDRFGLLAGFVEPGETLEEAAVREVREEAG
                     IEIAKLRYVRSQPWPFPDSLMVAFTADWASGEARADGEEIVELRWC"
     gene            complement(815..>2547)
                     /locus_tag="VFL04_02325"
     CDS             complement(815..>2547)
                     /locus_tag="VFL04_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HET7838568.1"
                     /translation="DKTGTLTRNRMAVVAFSADGDSGEAAQLDGSRPGHRLFLEALAL
                     CNDASLGPPDSSCEDPAPVGDPTEVALLEVADANGVCREDLVAAAPRIGELPFDSTRK
                     MMSTVHARGGGRIVMVKGAADSLLGRCDRVLVGGRELPLDAGRAGSILAEADEMAGRA
                     LRVLGAAYRRLEPAEEARGEGLERGLVFLGIVGMIDPPRLEVRESVAACRASGIGVAM
                     ITGDHKATALAIAMELGIATGESEAISGSEIDALGDEEFAARAKGTRVFARVSPEHKV
                     RIVKAFRAAGHVVSMTGDGVNDAPSLKAADIGVAMGITGTDVAKGASDMVLADDNFRT
                     IVRAIEEGRNIYNNIRKAILYLLSCNAGEIVAVFSAVLVAAPIPLLPIHILWVNLVTD
                     TFPALALGMDPGDPAIIRERPRDPKESLFSRGGGVFIALNGLLVGCLTIAAFWLGMGR
                     APQGVEPLQYARTMAFAVLSLSQLFHAFNLRHARSSLFSVGVLGNPWLAGAFILGAFL
                     QVVVIALPPLAAFFRVQSLDPGSWAIAAGLSASTVLANEAAKLGSRLFGGRGRAGTGR
                     EGERGLAESR"
BASE COUNT          377 a          889 c          949 g          332 t
ORIGIN      
        1 ggcaccagcg gagctccact atctcctcgc cgtcggcccg ggcctcgccc gaggcccagt
       61 cggctgtgaa ggccaccatg agggagtcgg ggaagggcca gggctgggag cgcacatagc
      121 ggagcttggc gatctcgatg ccggcctcct cgcgcacctc gcgcaccgcc gcctcctcga
      181 gggtctcgcc gggctcgacg aagccggcga ggagaccgaa gcggtcgggg gggaagcggg
      241 cgttgcgcgc caagagggcc tcctggccct gcctgacgag gacgatgacg gccggggaga
      301 tccgcgggaa gtgctcacgg ccgcagacgg ggcagacgcg gctgccgccg ccctcccggt
      361 actcgggctc ggccgaaaca tggcccgccg gatccccggg cttggccagt ggggcgtcga
      421 caaggccctc gccgcaggcg ccgcagcgca gggcgcccga aagccagtgc aggagggcga
      481 ggccggtgag ggcgggccgc atggcctcgg catcgaagct gccgatgacc tgtctgaccg
      541 gcacccgcgc gaggccaagg gcgagggcgg cggcggcccc cgcggggtcg aggaggcggg
      601 cctcgtagcg cccgccgccg cgcgcgaagc ggcgcgagag gacggggacg atggcgtcgg
      661 cgaccccggc gggcacggcc ggcccgggga cgtcggccct agagccgccc ccggggacat
      721 cagtcgcgga gttgtccccg gggatcagga tgtccaggcc ctcgaagagg aagatctgcg
      781 tggtgctttc cattgccccg cacgatacac cggctcagcg ggactcggcc agacctcgct
      841 ccccctccct ccccgtgccg gcccggcccc ttccgccgaa gagcctcgag cccagcttgg
      901 ccgcctcgtt cgcgaggacg gtgctggccg aaagcccggc ggcgatcgcc catgagcccg
      961 gatcgaggct ctggaccctg aagaaggccg ccagaggcgg cagggcgatg accacgacct
     1021 ggaggaaggc gcccaggatg aaggcccccg ccagccaggg attgcctagg acgccgaccg
     1081 agaacagcga gctcctcgca tgcctgaggt tgaaggcgtg gaagagctgg gagaggctca
     1141 ggacggcgaa ggccatcgtg cgcgcgtact ggaggggttc gacgccctgg ggggcccggc
     1201 ccatccccag ccagaaggcc gcgatggtca ggcagccgac gaggaggccg ttcagggcta
     1261 tgaagacgcc cccgccccgc gagaagaggc tctccttggg atccctgggc ctctcccgga
     1321 tgatcgcggg gtcgccgggg tccatgccga gggccagggc agggaaggtg tcggtcacga
     1381 ggttcaccca gaggatgtgg atcggcagga gggggatcgg cgccgccacc aggacggccg
     1441 agaagaccgc gacgatctcc ccggcgttgc acgacaggag gtagaggatg gccttgcgga
     1501 tgttgttgta gatgttccgc ccctcctcga tggcccgcac gatggtcctg aagttgtcgt
     1561 cggcgaggac catgtccgag gcgcccttgg ccacgtcggt gccggtgatc cccatcgcga
     1621 cgccgatgtc ggcggccttg aggctggggg cgtcgttgac cccgtccccg gtcatggaca
     1681 ccacatggcc ggctgcgcgg aaggccttca cgatcctcac cttgtgctcg ggcgagaccc
     1741 gggcgaagac cctcgtcccc ttcgccctgg ccgcgaattc ctcgtcgccg agggcgtcga
     1801 tctcgcttcc cgagatggcc tcggactcgc ccgtggcgat gccgagctcc atcgcgatgg
     1861 cgagggccgt ggccttgtgg tcgcccgtga tcatcgcgac gccgatcccc gaggccctgc
     1921 aggctgccac cgattccctc acctcgagcc ggggcggatc gatcatgccg acgattccga
     1981 ggaataccag gccgcgctcg aggccctcgc cgcgggcctc ctcggcgggc tcgagccgtc
     2041 ggtaggcggc cccgaggacc ctgagggccc tgccggccat ctcatcggcc tcggcgagga
     2101 tggagccagc ccgtccggcg tcgaggggaa gctccctgcc cccaaccagg acccggtcgc
     2161 agcggccgag aaggctgtcg gccgccccct tcaccatgac gatccgccct ccccccctcg
     2221 cgtggacggt gctcatcatc ttccgggtcg aatcgaaggg gagctcgccg atgcgcgggg
     2281 ccgcggcgac gaggtcctcg cggcagaccc cgttggcgtc ggccacctcg agcagggcga
     2341 cctcggtggg atcaccgacg ggggcagggt cctcgcagga ggagtcgggc gggccgaggg
     2401 aggcgtcgtt gcagagggcc agggcctcga ggaagagcct gtggcccggc ctcgagccgt
     2461 cgagctgggc cgcctcgccc gagtcgccgt cggcggagaa ggccacgacg gccatcctgt
     2521 tccgcgtgag ggtgcccgtc ttgtccg
//
LOCUS       DASSQV010000134         3199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_407069, whole genome shotgun sequence.
ACCESSION   DASSQV010000134 DASSQV010000000
VERSION     DASSQV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407069"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2260)
                     /locus_tag="VFL04_02330"
     CDS             complement(<1..2260)
                     /locus_tag="VFL04_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7838569.1"
                     /translation="MATVLLERGVSVWNPPLLIPALNLLFGLVALFAALLSARSYIEA
                     RSRRGLLLGASMFAYSLGILAAAAYSQAGDPNASSAAYVSMAAMSGLFALSIVAGRRL
                     RLPVDPGARRRQLAAAFSPVLAVLLVLGLLIARRAVPVFLLEGMGPTAFGSSACLATA
                     SLFAVSAVLLLARRGARALRFELWCGLGLALIALGLVGQCLGTSAGDRLNWLGRLTGY
                     AGVIYVLVAAISSMRRSGAWLLPVERALHESEETFRVLAEMSSAAFWLYQDEALVYVN
                     DAAATLSGYTKAELLGMRFWDIAHPEHAESLRELGRGIQEGKAGASKLEVMLRRKDGE
                     TRWAELSSGKTEYHGRPAGIASMYDITERKRTEDYLRRQAMILEAISDAIIVTDTSFR
                     IRSWNTRAERLYGWTEAEVLGRPAKEVLESELLGIDRAEAYRRLESGESVNMVSYQRT
                     KGGDRLYVLGYTVPLRDARGAITGYVAINHDETARKSAEVALLASEERLKFHLENSPL
                     AVVEWDSDFVVTQWSLEAERIFEWKKEEVLGRRIDQLDLILVDDLPIVERTMERLVSG
                     RERTVVSSNRNIAKSRRILDCTWYNSVLLDDKGRMASVMSLVQDNTELKHRQEEIAAQ
                     AAKLERANEELRSNNEELVEVSRSLGEARSYLDSLITYANAPIIVWDAHFAITRFNQA
                     FERLSGYRAEEVLGRRLSVLFPPSSQEEALAKIRKTLEGEQWESVEIPILYRHGGVRT
                     ALWNSANIYGESG"
     gene            2508..>3199
                     /locus_tag="VFL04_02335"
     CDS             2508..>3199
                     /locus_tag="VFL04_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HET7838570.1"
                     /translation="MAHRIRNTALIALILIAALSLTGAFAENPPAAPATVRDLNEQTV
                     MGLAWMQKAAEYRELCYQAYNLATMIVDKAIASAKPGDQPLAIISDLDESLLDNSAYD
                     VGFIGTNEAFSGKTWTQWENAVKAEAMPGAAEFLTGIAQRKVEVFYVTNRDMPGLEGT
                     IRNLQALGFPFADVKHVLVSTGSSDKQPRFDMVAKDFHVVLYMGDNANDLPIGTYHKN
                     MKDRNAIVDQNR"
BASE COUNT          575 a         1083 c         1026 g          515 t
ORIGIN      
        1 cgccggactc gccgtagatg ttcgccgagt tccacagggc tgtgcggaca cccccgtgtc
       61 tgtagaggat gggtatctcc accgactccc actgctcccc ctccagggtc ttcctgatct
      121 tggccagggc ctcctcctgg ctcgagggag gaaagaggac gctcaggcga cggccgagga
      181 cctcctcggc ccgatagccc gagaggcgct cgaaagcctg gttgaacctc gtgatggcga
      241 agtgcgcgtc ccagacgatg atcggggcgt tggcgtaggt gatcaggctg tcgaggtagc
      301 tgcgcgcctc gcccaaggat cggctgacct cgacgagctc ctcgttgttg ctgcgcagct
      361 cctcgttcgc gcgctcaagc ttcgcggcct gggcggcgat ctcctcctgt ctgtgcttca
      421 gctcggtgtt gtcctggacc agggacatga cggaggccat cctccccttg tcgtcgagga
      481 ggaccgagtt gtaccaggtg cagtcgagga tcctccgcga cttggcgatg ttgcggttcg
      541 aggacaccac ggtcctctcc ctgcccgaga cgagcctctc catggtgcgc tcgacgatcg
      601 gcaggtcgtc gacgaggatg aggtccagct ggtcgatccg cctgccgagg acctcctcct
      661 tcttccactc gaaaatgcgc tcagcctcca gcgaccactg ggtcacgacg aaatccgaat
      721 cccactccac gacggcgagg ggggaattct cgagatggaa cttgaggcgc tcctcgctcg
      781 ccagaagggc cacctcggcg gatttcctcg cggtctcgtc gtggttgatc gcgacatagc
      841 ccgtgatggc tcccctcgcg tcgcgcaggg ggacggtgta gccaaggaca tagagcctgt
      901 cgccgccctt ggtgcgctga taggacacca tgttcaccga ctccccgctc tccagacgtc
      961 ggtaagcttc ggccctgtcg atgcccagga gctcggattc gaggacctcc ttggccggcc
     1021 tgcccaacac ctcggcctcg gtccagccgt agagcctctc ggcccgggta ttccacgacc
     1081 tgatcctgaa cgaggtatcc gtgacgatga tggcgtcgct tatcgcctcg aggatcatcg
     1141 cctggcggcg gaggtagtcc tcggtgcgct tcctttcggt gatgtcgtac atgctcgcga
     1201 tacctgcggg ccggccgtgg tattcggtct tccccgagct cagctcggcc cacctcgtct
     1261 cgccgtcctt cctcctcagc atcacctcga gcttcgatgc ccctgccttc ccctcctgga
     1321 tgccgcggcc aagctcgcgc aggctctcgg cgtgctcggg atgggcgatg tcccagaacc
     1381 tcatgccgag cagctcggcc ttcgtgtaac cgctcagggt cgccgcggcg tcgttcacat
     1441 agaccagggc ctcgtcctgg tagagccaga aggcggcgct cgacatctcg gcgaggaccc
     1501 ggaaggtctc ctcgctctcg tgcagggccc gctcgaccgg caggagccat gcgccgctcc
     1561 gcctcatgga cgagatggcg gcgacaagga cgtagatgac ccctgcgtag cccgtcagcc
     1621 gccccagcca gttgagcctg tcccccgccg aggtcccgag gcactggccc accaggccca
     1681 gggctatcag ggccagtccc agcccgcacc agagctcaaa gcgcagagcc ctcgcccccc
     1741 tcctggccag gaggaggacg gccgagacgg cgaagagcga ggccgtggcc agacaggccg
     1801 agctcccgaa ggccgtcggc cccatgccct caaggaggaa gaccgggaca gcccggcgcg
     1861 ctatgagaag gccgaggact aggaggactg ccaggacagg actgaaggct gcggcaagtt
     1921 gccttcgcct cgcgcccggg tcgacgggga ggcggagcct ccttccggcg acgatggaga
     1981 gggcgaaaag ccccgacatg gcggccatcg acacgtaggc ggcgctgctg gcgttcggat
     2041 cgccggcctg ggaataggcc gcagcggcca ggatgcccaa ggaataggcg aacatggagg
     2101 ccccgaggag cagccccctc ctcgaccgcg cctcgatgta gctcctcgcc gaaagcaggg
     2161 ccgcgaagag ggcgacaagg ccaaacagga ggttgagggc cggaatgagc aggggagggt
     2221 tccacaccga gaccccgcgc tcaaggagga cggtcgccaa gaggcctgcg aaggccggaa
     2281 gcgggagcag tgtccagcgt ggcgcccaag aaactcttcc cgcagtcctc accgccatgc
     2341 atcctccagg aattgggctc tgcatcgccg tcgcgtgagc ggcggctccg gcgggaatcc
     2401 tacaatgggg aattggggca ggcaagccgg gatcggagcg gccgctgttg acaggccgga
     2461 ggcgcatgac tagttttgcc tatcaacatc tcatgaagga ggcccgcatg gcccacagga
     2521 tcaggaacac cgccctaatc gccctcatcc tcatcgccgc cctctcgctg acaggcgcct
     2581 tcgcggagaa cccgcccgcg gcgccggcga cggtccgcga cctgaacgag cagaccgtca
     2641 tgggcctcgc ctggatgcag aaggcggccg agtaccgcga gctctgctat caggcctaca
     2701 acctcgccac gatgatcgtc gacaaggcca tagcctcggc caagcccggc gaccagcccc
     2761 tggccatcat ctccgacctc gacgagagcc tcctggacaa ctctgcctac gacgtcgggt
     2821 tcatcggcac aaacgaggcc ttctcgggca agacctggac gcagtgggag aacgccgtca
     2881 aggccgaggc gatgccgggg gccgccgagt tcctgacggg catcgctcag aggaaggtcg
     2941 aggtcttcta cgtcacgaac cgggacatgc cggggctcga ggggacgatc aggaacctgc
     3001 aggccctggg cttccccttc gccgacgtga agcacgtcct ggtcagcacg ggctcgagcg
     3061 acaagcagcc gcgcttcgac atggtcgcga aggacttcca cgtagtgctc tacatgggag
     3121 acaacgccaa cgacctcccc atcggtacct accacaagaa catgaaggac aggaacgcga
     3181 tcgttgacca gaaccgtga
//
LOCUS       DASSQV010000135         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1400398, whole genome shotgun sequence.
ACCESSION   DASSQV010000135 DASSQV010000000
VERSION     DASSQV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400398"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VFL04_02340"
     CDS             <1..1365
                     /locus_tag="VFL04_02340"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HET7838571.1"
                     /translation="STYFVIAWHTRRINESPSIAALGIAYLFVAILDVFHTLAYQGMG
                     VFTGYSYPANQVWVVARLMEALSLLGFSFVRRLRGRGLALFIGAYIITTAAGLASIFI
                     FRIFPVCFVAGLGQTRFKMVMEAVVISVLVLASLVLWRRRGAFPGEIYENLQLSVILT
                     AVSEFAFTLYVSNSDWVNMTGHLLKIASFYLVYRSIVVTGLERPQELLYSRLKGREEE
                     LRRANEAKDTLISILGHDLRSPLAGIQGVASMLATDPSLAGEGELGTWLKEIAKSAGS
                     SLELAENVLSWARCQSGELLPAMSPVNPAKILRTEAELLKEAARSKGVCLELVASEPP
                     SVLADPNMLSTIVRNMLQNAVKFTPSGGRVVARATGSSSECVVEVEDTGTGMDEDELG
                     RLFRVDGRLRGVGTAGERGTGFGLVVASEFSRKMGARLEVKSAKGIGSTFRIRIPRSP
                     VEIL"
     gene            complement(1362..1826)
                     /locus_tag="VFL04_02345"
     CDS             complement(1362..1826)
                     /locus_tag="VFL04_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET7838572.1"
                     /translation="MRVEYINPFVEAAFNVLREVLQSDVRRGELYLKSTSTPVMGVAA
                     IVGLAGDVEGRVLFDMDRPTALQIASTMNGEELTSLDDLVKATITELANMITAQAVTK
                     LHDLGFRFDLTPPAIFTGDNMEVSNSDVEALIVPMETVHGKIEINVAIRERA"
     gene            complement(1861..>2416)
                     /locus_tag="VFL04_02350"
     CDS             complement(1861..>2416)
                     /locus_tag="VFL04_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET7838573.1"
                     /translation="FWSAETGRLWDDDYAASVLAVLPDIESRSINVWNPGCGKGYETW
                     SLACIMRRRYPEARIRIWANDSDLLAISMAPNMVSAENAPPYYADFLTKGRNGLAFIQ
                     SIRDSIFFEFHDIMNANPLPPLDLILCRDVLSFVPPIDQKRLIGDFIDKLKPNGIVFT
                     GGNERLGEGWAFVGSEAVPAFRKA"
BASE COUNT          406 a          764 c          809 g          437 t
ORIGIN      
        1 tcgacctatt tcgtcatcgc gtggcacacg aggaggatca acgagagccc ctccatagcc
       61 gcactcggca tcgcctacct cttcgtcgcc atcctcgatg tcttccacac cctcgcctac
      121 cagggcatgg gggtcttcac cggctactcc taccccgcca accaggtctg ggtggtcgcc
      181 cgcctcatgg aagccctgag cctcctcggc ttcagcttcg tccgcagatt aagggggagg
      241 ggccttgccc tcttcatcgg agcctacata atcaccacgg cggcaggcct cgcctcgatc
      301 ttcatcttcc ggatcttccc cgtctgcttc gtcgcgggcc tcggccagac ccggttcaag
      361 atggtcatgg aggccgtcgt aatctcggtc ctggtcctcg cctcccttgt cctctggaga
      421 aggagggggg ccttccccgg cgagatctac gagaacctcc agctctcggt catcctgacc
      481 gccgtctccg agttcgcctt caccctgtac gtctccaact ccgactgggt caacatgacg
      541 ggccacctcc tgaagatcgc ctcgttctac ctggtctacc gctccatcgt ggtcaccggg
      601 ctcgagaggc cgcaggagct gctctactcg aggctcaagg gcagggagga ggagctgagg
      661 cgcgccaacg aggcgaagga caccctgatc tccatcctcg gccacgacct caggagcccc
      721 ctcgccggga tccagggcgt ggcgagcatg ctggccaccg acccctccct ggcgggcgaa
      781 ggcgagctgg gaacctggct gaaggagatc gccaaatcgg cgggctcctc cctcgagctc
      841 gccgagaacg tcctctcctg ggcccgctgc cagagcggag agctcctgcc ggcgatgagc
      901 cccgtgaacc ccgccaagat cctcaggacc gaggccgagc tcctcaagga ggctgcgcgg
      961 agcaaggggg tctgcctcga gctggtcgca tccgagccgc cctcggtgct cgccgatccg
     1021 aacatgcttt cgacaatcgt ccgcaacatg ctccagaacg cagtgaagtt caccccctcg
     1081 ggcggtcggg tcgtggcgag ggccacgggc agctcgagcg aatgcgtggt cgaggttgag
     1141 gacacgggca cggggatgga cgaggatgag ctcggccggc tcttccgcgt cgacggccgg
     1201 ctccgcggcg tgggcacggc aggagagagg gggacgggct ttggacttgt ggtggcgtcg
     1261 gagttctcga ggaagatggg ggccagactc gaggtgaaaa gcgcgaaggg gatcggatcc
     1321 accttccgga tccggatccc ccgcagtcct gtcgagattc tctaggcgcg ctcgcggatc
     1381 gcgacgttga tctcgatctt gccgtggacg gtctccatcg gcacgatcag ggcctcgacg
     1441 tcggagttgg agacctccat gttgtccccc gtgaagatcg cgggaggcgt caggtcgaag
     1501 cggaagccga ggtcgtggag cttggtcacc gcctgggcgg tgatcatgtt cgccagctcg
     1561 gtgatggtgg ccttgaccag gtcgtcgagg ctcgtgagct cctcgccgtt catggtcgag
     1621 gcgatctgca gggcggtcgg cctgtccatg tcgaagagga cgcggccctc gacgtcgccc
     1681 gcgaggccca cgatcgccgc gacgcccatc accggggtcg aggtggactt gagatacagc
     1741 tcgccacggc gcacgtcgga ctgcagcact tcacggagta cgttgaacgc agcttcgacg
     1801 aagggattga tgtactcgac tctcatgccg cactcctcga atcggccggt atccggctct
     1861 tcatgccttg cggaaggccg ggacggcctc cgagccgacg aaggcccatc cctcgccgag
     1921 gcgttcgttg cccccggtga agacgatgcc gttcggcttc agcttgtcga taaaatcgcc
     1981 tatgagcctc ttttggtcaa tcgggggaac gaaggatagg acgtcccggc agaggatgag
     2041 gtcgaggggc ggaagggggt tcgcgttcat gatgtcgtgg aactcgaaga agatcgagtc
     2101 gcggatcgac tggatgaagg cgagtccgtt gcggcccttc gtgaggaagt cggcgtagta
     2161 gggtggcgcg ttctcggcgg agaccatgtt gggcgccatc gagatcgcga ggaggtcgga
     2221 gtcgttcgcc cagatgcgga tgcgggcctc tgggtagcgg cggcgcatga tgcaggcgag
     2281 ggaccaggtc tcgtagccct tgccgcagcc ggggttccag acgttgatgc tccgcgactc
     2341 gatgtcggga aggacggcca ggacggaggc ggcgtaatcg tcgtcccaga gcctgccggt
     2401 ctcggctgac cagaag
//
LOCUS       DASSQV010000136         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2271894, whole genome shotgun sequence.
ACCESSION   DASSQV010000136 DASSQV010000000
VERSION     DASSQV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271894"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VFL04_02355"
     CDS             <1..1008
                     /locus_tag="VFL04_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HET7838574.1"
                     /translation="RSWESLDIVKVLREGRGFLLISNLALAGFQRRMGADVGVKPGEE
                     MLAAVQSAKDAGIPYSFCDREVQVTLKRAWAKSGLWNRAKLMAALLESSFFAEKLSAD
                     QIEKLKERNELEEMMAELSSFLPSVKEVLIDERDRFLASRIYEAEGSKVLAVVGAGHL
                     NGIESWLSRLAAGEASSDTSDIQSVPPPSKWGKVIGWAIPAVIVGLIILGFFKAGVSA
                     SLALLVKWILLNGSLAALGSLLCMAHPLTILASFVMAPIATLNPFVGVGLFAGLAEAF
                     LRRPRVRDLENLAEDVVSIKGFYKNRVTHILLVFFLSSLGGALGNFIALPFLASGAFG
                     K"
     gene            1113..1391
                     /locus_tag="VFL04_02360"
     CDS             1113..1391
                     /locus_tag="VFL04_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838575.1"
                     /translation="MPLVFELLLWFGLVAAIAFGARSFLKTLIKDRAEMRTVNSAVSA
                     SSDLESLKRRIEVLEAKVEERDLNIRKIRDELNFVSRMLEDKTTGPGA"
     gene            1404..1895
                     /locus_tag="VFL04_02365"
     CDS             1404..1895
                     /locus_tag="VFL04_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HET7838576.1"
                     /translation="MDNCPCGSGREYESCCGPYVSKRALAPTAEALMRSRYSAYARHQ
                     IDFIVDTCVRDEEHGIDLEATKRWSEKSEWLGLKIIRTEKGGPGDDKGLVEFSATYVL
                     DGLREEHHEVSSFVKREGAWLYEEGEMITETVVRAAPKVGRNDPCPCGSGKKYKKCCG
                     AAA"
     gene            complement(1941..>3467)
                     /locus_tag="VFL04_02370"
     CDS             complement(1941..>3467)
                     /locus_tag="VFL04_02370"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011443.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HET7838577.1"
                     /translation="VNPQTEYIFDRFGLELPLFLPDLVPKVEYFLESGAPTVRDIEPL
                     WEALALMGKAERKVLPIVDAEGRYRSMLHYGAFAQNMLAKINPHKKAVIPTSVGHLVK
                     TIKAQALVTFGEDIFFKARIIVAALETEAFREHLKGEPIENTVVLVGNREDVQRIAIE
                     AGVRAIIVTNGNLLGKGLRELAEEHRVSILVSPYDTSSTSLLALYSTPVWTMGDTTIV
                     AVQAGDTLKTARAAIAVSPSRSVPVVDGEGRVMGIFAEGDLIREPHVEVILVDHNELS
                     QAVEGIEHYRILEVIDHHRLGALTTKYPITFINRVVGSTSTIVAGMYRELKVPLPRPI
                     ASILLCGILSDTVILKSATTTEVDREMAEYLSNITDLEIEELGRDIQGSASAASRMPA
                     ASIIRLDRKEYQATGKKISVSQIEVTNTEEVQARSAEIIAALKTMRAEGGFYLSALMV
                     TDVTKLTSILLVDGEKDFVSQVNYPRLESGVFMLSDILSRKKQLMPAIFEMVEKAVER
                     "
BASE COUNT          571 a         1150 c         1123 g          623 t
ORIGIN      
        1 aggtcatggg agagcctgga catcgtcaag gtcctcaggg agggcagggg cttcctcctc
       61 atctccaacc tcgccctcgc gggtttccag cgccgcatgg gcgccgatgt cggcgtgaag
      121 cctggcgagg agatgctcgc cgcggtgcag tcggccaagg acgccggaat cccctattcc
      181 ttctgcgacc gcgaggtcca ggtgaccctc aagcgggcct gggccaaatc gggcctatgg
      241 aaccgggcca agctcatggc ggccctcctc gagtcctcct tcttcgccga gaagctttcg
      301 gccgaccaga tcgagaagct caaggagcgc aatgagctcg aggagatgat ggccgagctc
      361 tcgagcttcc tcccctcagt gaaagaggtc ctgatcgacg agcgggaccg cttcctcgcg
      421 agcaggatct acgaggccga gggctcgaag gtcctcgccg tcgtcggagc cggccacctc
      481 aacgggatcg agtcctggct cagccggctc gccgcggggg aggcctccag cgacaccagc
      541 gacatccaga gcgtgccgcc gccttccaag tggggcaagg tgatcggctg ggcgattccc
      601 gccgtcatcg tcggcctcat catcctcggc ttcttcaagg ctggggtgtc ggcaagcctc
      661 gccctcctcg tcaagtggat cctccttaac gggagcctcg ccgcccttgg atcacttctc
      721 tgcatggccc accccctcac catcctcgcc tcattcgtga tggccccgat cgccaccctc
      781 aatcccttcg tcggcgtcgg tctgttcgcg ggtctcgccg aggccttcct cagacggccc
      841 agggtgcggg acctcgagaa cctggcagag gacgtcgtgt ccatcaaggg cttctacaag
      901 aaccgagtga cccacatcct cctggtcttc ttcctctcga gcctcggggg tgcgctcggc
      961 aacttcatag ccttgccctt cctggcctca ggcgccttcg gcaagtaagg cggggcgggg
     1021 ccgggagcat tgacccgcgg cccccacgga ctgcatcatt gcgtggcggc ggcgccggac
     1081 ccggtggcgc cgccttccat cggaggttgg tcgtgcccct cgtgtttgag ttgctccttt
     1141 ggttcggcct ggtcgccgca atcgccttcg gcgcgcgaag cttcctgaag accctgatca
     1201 aggacagggc cgagatgcgg accgtgaact cggccgtctc ggcatcctcc gacctcgaat
     1261 ccctgaagcg tcggatcgag gtccttgagg ccaaggtcga ggagcgcgac ctgaacatcc
     1321 ggaagatccg cgacgagctc aatttcgtct cgaggatgct cgaggacaag accaccggcc
     1381 ccggagcctg aaaggaattt cgaatggata actgcccctg cggctcaggc cgcgaatacg
     1441 agtcctgctg cggcccctac gtctccaagc gcgccctcgc ccccacggcc gaggccctca
     1501 tgcgcagccg ctactcggcc tacgcccggc accagatcga cttcatcgtc gacacctgcg
     1561 tccgcgacga ggagcatggc atcgacctcg aggcgaccaa gaggtggagc gagaagtccg
     1621 agtggctggg actgaagatc atccgcacag agaagggcgg accgggggac gacaagggcc
     1681 tcgtcgagtt ctcggcgacc tacgtcctcg acggccttcg cgaggagcac cacgaggtct
     1741 ccagcttcgt gaagcgcgag ggggcttggc tctacgagga gggcgagatg atcactgaga
     1801 ccgtggtccg cgccgccccg aaggtcggcc gcaacgatcc atgcccatgc ggaagcggca
     1861 agaagtacaa gaagtgctgc ggagccgccg cctaaagcct cgccgggctg gggccggcgg
     1921 tccgccgccg cgccccaggc tcagcgctcg acggccttct cgaccatctc gaagatcgcc
     1981 ggcatgagct gcttcttgcg cgacaggatg tcgctcagca tgaagacccc gctctcgagc
     2041 ctcggatagt tcacctggct cacgaagtcc ttctctccgt cgacgaggag gatgctcgtg
     2101 agcttcgtca cgtcggtgac catgagggcc gacaggtaga agccgccttc cgcccgcatc
     2161 gtcttgagcg cggcgatgat ctcggccgac cgcgcctgga cctcctcggt gttcgtgacc
     2221 tcgatctggc tcacgctgat cttcttgccc gtcgcctggt actccttcct gtcgagcctg
     2281 atgatcgagg ccgcgggcat ccgcgaggcc gccgaggccg agccctggat gtcccgcccg
     2341 agctcctcga tctcgaggtc ggtgatgttc gaaaggtatt cagccatctc cctgtccacc
     2401 tcggtcgtgg tcgccgactt gaggatgacc gtgtcggaga ggatgccgca caggagtatg
     2461 gaggcgatgg gccgcggcaa ggggaccttc agctccctgt acatccccgc gactatcgtg
     2521 ctcgtcgagc cgacgacgcg gttgatgaag gtgatggggt acttggtcgt gagcgcgccc
     2581 aggcggtggt ggtctatgac ctcgaggatg cggtagtgct cgatcccctc gacggcctgg
     2641 gacagctcgt tgtggtcgac aaggatcacc tcgacgtggg gctcccgtat caggtccccc
     2701 tccgcgaaga tgcccatgac ccttccctcc ccatcgacca cgggcaccga gcggctcggc
     2761 gagaccgcta tggcggcgcg agccgtcttc agcgtgtccc cggcctggac ggccacgatc
     2821 gtcgtgtcgc ccatggtcca gacgggggtc gagtagaggg cgaggaggga ggtgctcgag
     2881 gtgtcgtagg gcgagaccag gatggagaca cggtgctcct cggcgagctc ccgcaggccc
     2941 ttgcccaaca ggttcccgtt ggtgacgatg atcgcgcgca ccccggcctc gatcgctatg
     3001 cgctggacgt cctccctgtt ccccacgagc accacggtgt tctcgatcgg ctcacccttc
     3061 aggtgctcgc ggaaggcctc tgtctcaagg gccgccacga tgatcctggc cttgaagaag
     3121 atgtcctcgc cgaaggtcac cagggcctgg gccttgatgg tcttgacgag gtggcccacg
     3181 ctcgtgggaa tgacggcctt cttgtgcgga ttgatcttcg cgagcatgtt ctgggcgaag
     3241 gccccgtagt gcagcatcga gcggtagcgt ccctcggcgt ccacgatggg aagcaccttg
     3301 cgctcggcct tgcccatgag ggcgagggcc tcccagaggg gctcgatgtc cctgacggtc
     3361 ggggccccgc tctcgagaaa gtactcgacc ttgggcacga ggtcgggcag gaaaagcggc
     3421 agctcgaggc cgaagcggtc gaagatgtac tcggtctggg ggttcac
//
LOCUS       DASSQV010000137         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_904771, whole genome shotgun sequence.
ACCESSION   DASSQV010000137 DASSQV010000000
VERSION     DASSQV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904771"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFL04_02375"
     CDS             <1..307
                     /locus_tag="VFL04_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET7838578.1"
                     /translation="YHAIMLAMIELTVVVSVRTVGTLLVFGMLLAPAATAALFARKIS
                     VMIALASAVGAVSVYLGLLASYHLDLAAGASVTLVATIVFFAALMVRRLLPTAGGQR"
     gene            304..1125
                     /locus_tag="VFL04_02380"
     CDS             304..1125
                     /locus_tag="VFL04_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7838579.1"
                     /translation="MSGAAVRDSALSARGLAIGYAEETVVEGIDFELPRGKTLALVGS
                     NGSGKTTLLKTAAALLPPVSGEMSVLGARPGAFPARVAYLGQFQSSGFTLPLRAIDVV
                     RMARFASLGLTRRAGPEDERMVREAMEIMGVSDLANEGLRTLSGGQRQRVFIAQALAR
                     GADLILLDEPASSLDGASRETYRSILREACASGRSAAIATHDVEEAAGCDYTMLLARK
                     VVAFGESAAVLTAETLLSTFGVVARSDRSGLLVLEHEHGHDCLDPDHEGPEDQRR"
     gene            1205..>3104
                     /locus_tag="VFL04_02385"
     CDS             1205..>3104
                     /locus_tag="VFL04_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838580.1"
                     /translation="MVGIPAPRMVLLLTLSWLVAALSAQTTGPELPVARVVLFSSGVG
                     YFEHRGQVSGDASVALRFSSDEVNDALKSLVIWDASTASPSVSYPSEESLDRALKGFR
                     VDLSGSPRIAELLARLRGAEVSIDTPDTITGKIVGVEARPDKEGKTQLPYLVLATEGG
                     IKAVSLNELAAIRFGDKRISEDFDRALALILSAQDRLRRVLDIRLPGTGTRQARLGYV
                     VAAPVWKVSYRLDMSGDKPFLQGWAIVDNPTDQDWTNVNLSLVSGRPVSFIQDLYTPL
                     YVDRPVIPLAIAGTAAPRTFDSGMAPSPEEELMAEAEPAAKMAAPAPAPPMSRAQKAA
                     GPGAGFDLASSAVETTQARAAGDQFEFTVKKPVTLERRRSAMIGLVSGEIALEKLSVF
                     SPGSGSRNPMLGARISNSTGTKLPAGPITIFDGGVYAGDALLDFLPEKEKRLIVFGTD
                     LSVTGDVTATSSQETIGVKVSKGVLIFSRRTTWTRGYQFRNSGLQPKKVLVEHPITGG
                     AELFEPKVFDEKTDSVYRFLVSVPAGGQAKLDVKERSPAQESVALSSLSTDSFLRYSS
                     SQEIPQAIRDALKQAMDLRRKVDDSRRALSDLQARKNEISNDQARIRQNLGAVGRDTS
                     QGQQYLKRL"
BASE COUNT          493 a         1148 c          988 g          475 t
ORIGIN      
        1 ctaccacgcg atcatgctcg ccatgatcga gctcacggtc gtcgtctcag tccgcaccgt
       61 ggggaccctc ctagtcttcg ggatgctcct ggccccggcc gccaccgccg ccctcttcgc
      121 gcgcaagatc tcggtcatga tcgcgctggc ctcggctgtc ggcgcggtct cggtatatct
      181 cggcctcctg gcgagctacc acctcgacct cgcggcgggg gcctccgtga ccctggtcgc
      241 gaccatcgtg ttcttcgccg ccctcatggt aaggcggctt ctgccgacgg cgggaggcca
      301 gcgatgagcg gggccgcggt gcgggacagc gccctcagcg ccagggggct cgcgatcggc
      361 tacgctgagg agaccgtcgt ggagggcatc gatttcgagc tgccccgcgg caagaccctc
      421 gccctcgtcg ggtccaacgg ctcgggcaag accaccctcc tcaagaccgc cgccgccctc
      481 cttcccccgg tctcgggcga gatgtcggtg ctcggagcca ggcctggggc cttcccggcc
      541 cgcgtggcat acctgggcca gttccagagc tcgggcttca ccctcccgct ccgggccatc
      601 gatgtcgtcc ggatggcgag gttcgcctcc cttggcctta cccgcagggc cggccccgaa
      661 gatgagagga tggtcaggga ggccatggag atcatggggg tctccgacct cgccaacgag
      721 ggcctgcgca ccctttccgg cggccagcgc cagcgggtct tcatcgccca ggccctcgcc
      781 aggggcgccg acctgatcct cctcgacgag ccggcgtcca gcctcgacgg ggcctcccgg
      841 gagacctacc gctccatcct cagggaggcc tgcgcctcgg gcaggagcgc ggccatcgcg
      901 acccacgacg tcgaggaggc ggcgggctgc gactatacga tgctgctcgc gaggaaggtg
      961 gtagccttcg gcgagagcgc ggccgtgctc acggccgaga ccctcctgtc caccttcggc
     1021 gtggtggcgc gttccgaccg ctccggcctc ctcgtgctcg agcacgaaca cggccacgac
     1081 tgtctggatc cggaccacga ggggccggag gatcagaggc gctgagcccc caggcggcca
     1141 cgggcgggtc ggggggcccg gcgggcatgt gctatacttc cgggaagtct cccaggaggt
     1201 cagcatggtc ggcattcccg cgccccgaat ggtccttctc ctcacccttt cctggctggt
     1261 cgcagcccta tcggcccaga ccacggggcc cgagctcccg gtggcccggg tcgtcctctt
     1321 ctcctcgggt gtgggctatt tcgagcaccg cggacaggtg agcggcgacg cctcggtcgc
     1381 cctccgtttc agctcggacg aggtgaacga cgccctcaaa tccctcgtga tctgggacgc
     1441 gtccacggcc tcgccctccg tcagctatcc ttccgaggaa agcctggaca gggcgctcaa
     1501 gggtttccgc gtcgacctct cgggctcgcc caggatagcc gagctcctcg cccggctgcg
     1561 cggagccgag gtctcgatcg acacgcccga caccatcacg ggcaagatcg tcggggtcga
     1621 ggcgaggcct gacaaggaag gcaagaccca gctgccctac ctggtcctgg ctaccgaggg
     1681 gggcatcaag gccgtctccc tcaacgagct cgcggccata cgtttcggcg acaaaaggat
     1741 ctccgaggac ttcgaccggg ccctcgccct catcctctcg gcccaggaca ggctcagaag
     1801 ggtcctcgat atcaggctcc ccggcacggg cacccgccag gccaggctcg gctacgtcgt
     1861 cgcggccccg gtctggaagg tgtcctaccg cctcgacatg tcgggagaca agcccttcct
     1921 ccagggctgg gccatcgtcg acaacccgac cgaccaggac tggacgaacg tgaacctctc
     1981 cctcgtcagc ggccggccgg tctccttcat ccaggacctc tacacccccc tctacgtcga
     2041 cagaccggtg atccccctcg cgatagccgg gacggccgcc ccccgaacct tcgactcggg
     2101 catggccccc tcccccgagg aggagctcat ggccgaggcc gagccggcgg ccaagatggc
     2161 cgccccggcc ccggcgcccc cgatgagccg agcccagaag gcagcggggc ccggagccgg
     2221 cttcgacctg gcctcgagcg ccgtcgagac cacccaggcc agggcggcgg gggaccagtt
     2281 cgagttcaca gtcaaaaagc ccgtcaccct cgagcgccgc aggagcgcga tgatcgggct
     2341 cgtctcgggc gagatagccc tcgagaagct ctcggtcttc agccccggct cgggctcgag
     2401 gaaccccatg ctcggcgcca ggatctcgaa ctcgacgggc accaagctcc cggccgggcc
     2461 catcaccatc ttcgacggcg gggtctacgc tggcgacgcc ctcctcgact tcctgcccga
     2521 gaaggagaag cgcctcatcg tcttcgggac cgacctttcg gtcaccggcg acgtgacggc
     2581 cacgagctcg caggagacca tcggcgtgaa ggtctccaag ggcgtcctga tcttcagcag
     2641 gaggaccacc tggacccgcg gctaccagtt caggaactcc ggcctccagc ccaagaaggt
     2701 cctcgtggag cacccgatca ccggcggtgc cgagctcttc gagcccaagg tcttcgatga
     2761 gaagaccgac agcgtctaca ggttcctcgt ctctgtcccc gccggcggcc aggccaagct
     2821 cgatgtaaag gaaaggagcc ccgcccagga gagcgtcgcc ctctcctccc tgagcacgga
     2881 ctccttcctc cgctattcct cttcccagga gatcccccag gccatccgcg atgccctcaa
     2941 acaggccatg gacctccgaa ggaaggtcga cgattcgcga cgggccctct cggatctgca
     3001 ggcgcggaag aacgagatct ccaacgacca ggcgaggatc cgccagaacc tcggcgctgt
     3061 gggaagggac acgagccagg ggcagcaata cctcaagcgg ctca
//
LOCUS       DASSQV010000138         3783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2148280, whole genome shotgun sequence.
ACCESSION   DASSQV010000138 DASSQV010000000
VERSION     DASSQV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148280"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..877
                     /locus_tag="VFL04_02390"
     CDS             119..877
                     /locus_tag="VFL04_02390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HET7838581.1"
                     /translation="MNILILERDEIGARLPRGDRRWRHVREVLRKGRGERIAAGIADG
                     ELGSATVLELDDGGMVLGFEPEREGEALAPLTLILGFPRPIQAARILRDLTSLGVARI
                     ELTGTQLGEKSYTQSDFFSKKQFRLPLLEGAEQAGNPRLPRVRTHWTLERCLAALDEP
                     EAGEPRAEAGAPAGPTRLFLHPYGDALDLGALGEFGAPVSLAIGSERGWTEAEAGALR
                     AAGFSPARLGRRILKTETATLAATIIVLSRLGLL"
     gene            1142..2329
                     /locus_tag="VFL04_02395"
     CDS             1142..2329
                     /locus_tag="VFL04_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7838582.1"
                     /translation="MRKLLLVVAVLMTISLLLAGCGPAAPKVIKIATQSPLSGGMSAN
                     GTDIKNGAQLGVEQLSDPLVKLGFKVELAPFDDQGNPDTGVANAKSIVSDPAILAVVG
                     HYNSGVQIPSSEEYHAAGLVNVSPANTNPKVTERGYLEVNRICGRDDVQGAVGAQFAK
                     SKNVKSAYVLHDKTAYGQGIAEYFKREAETLGIKVMGFAGTEEKANFDAILTPILASK
                     PEVIYFGGMYDQSSVLFKQARQKGYKGMFLSDDGFDSSDAAKIGGDALLQGAGTYYST
                     VSGPASVYPGTAKFIADFKAKFGADPQPFAAQGYDCAGIALKAIENAILANGKKLPTR
                     AAVANAVRALKDYQGITGVINFNAKGDLTKAKYFIIQVTSNDPAKWPMNKIDQTLDIA
                     PPQ"
     gene            2393..3556
                     /locus_tag="VFL04_02400"
     CDS             2393..3556
                     /locus_tag="VFL04_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7838583.1"
                     /translation="MRKFLGSEALRELILPLARMAVFVLGAGALFSAIMVVLALLARG
                     SGADQLLLSTQNTNILAYTLGNLPQVLIDGVTLGFVYAAIALGYTMVYGVLEFINFAH
                     SEIFAIGAFTGTEFLITMDRLGYLKSASPTAAYVYLAISLLIGMMAAGGVAVLMERTA
                     YRPLRDSPRLVPLISAIGVSFALQDVIRLVESLTTGQFYRVMPTFGSFDDRLPLFSIA
                     GVDVDIQVKSVVVIGAAVIMLVGLNYIVNATRVGKAIRSVAQDRNTAALMGIGVNGII
                     SLTFLIGGALGGAAGVLYSLKFTRIDPFVGFMPGIKAFAAAVLGGIGNLTGALLGGII
                     LGMLESFAGAYLGTFTMGNFGAEYKDMFAFLVLILVIIFRPTGLLGEQVSQKA"
     gene            3567..>3783
                     /locus_tag="VFL04_02405"
     CDS             3567..>3783
                     /locus_tag="VFL04_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838584.1"
                     /translation="MRFNLREALRDFWSLINRGKNPYTLSVGLLVVAAVLVYLSPRSI
                     PVFLLFAVAAGLPYFLEMRKPAKLAIAV"
BASE COUNT          629 a         1389 c         1130 g          635 t
ORIGIN      
        1 aatctcaggt acggatcccg tatcgtgcgt cgcgaatcga agcggtcttc gaaaagcgcg
       61 tatccggtcg tgcagggcga agctcggcac gacttttttt ttgttgtaga atcccgccat
      121 gaacatcctc atcctcgaga gggacgagat cggggcgagg ctcccgcgcg gcgaccgaag
      181 gtggcgccac gtgcgcgagg tcctgcgcaa gggcaggggc gagaggatcg ccgccggcat
      241 cgccgacggc gagctcggca gcgcgacggt cctcgagctc gacgatggcg gcatggtgct
      301 cggcttcgag cccgagcgcg agggcgaggc cctggccccc cttaccctga tcctcggctt
      361 ccccaggccg atccaggcgg cgcggatcct cagggatctc acgagcctgg gcgtggcgag
      421 gatcgagctc accgggaccc agctcggcga gaagtcctac acccagagcg atttcttcag
      481 caagaaacag ttccgcctgc ccctgctcga gggcgccgag caggcgggca acccgaggct
      541 gccgagggtc cgcacccact ggaccctcga gcgctgcctc gccgccctgg acgagcccga
      601 ggccggcgag ccccgcgccg aggcgggcgc cccggccggg ccgacgaggc tcttcctcca
      661 cccctacggc gatgccctcg accttggggc cctcggcgag ttcggcgccc cggtgagcct
      721 cgcgatcggc agcgagcggg gctggaccga ggccgaggcg ggagccctgc gcgcggcggg
      781 attctctcct gcccgactcg ggaggcggat cctgaagacc gagacggcca ccctcgcggc
      841 gacaataatc gtgctttcga ggctgggttt gctctagccg gcgccaaatc gtccttgact
      901 ccgcaaaatt ctcactctag actcgcgtta aacggtgccc cagcgtggca cctccgcctt
      961 tcgaatctgc accgcgcggc cacgaagccc gtttgggcct tccaactgtc ctccgcggac
     1021 tctcgaagga ggtacaccgg acctgtgtga gaccgtccat ctcgccgccc ccgctccgct
     1081 cttggcgcgc gttgcgcgtc gccggttatc ttccaaacag atgtccccgg agggaatgct
     1141 tatgcgaaag cttctcctag ttgtcgcggt cctcatgacg atcagccttc ttctggccgg
     1201 ctgcggaccc gccgcgccga aggtgatcaa gatcgccacc cagagccccc tctcgggcgg
     1261 catgtcggcc aacggcaccg acatcaagaa tggagcccag ctcggcgtcg agcagctctc
     1321 cgatcccctg gtgaagctgg ggttcaaggt cgagctcgcc ccctttgacg atcagggcaa
     1381 ccccgacacc ggcgtcgcca acgccaagag catcgtgtcc gacccggcca tcctcgcggt
     1441 cgtggggcac tacaactccg gcgtccagat cccctcctcc gaggagtacc acgccgccgg
     1501 cctcgtgaac gtgtctcccg ccaacaccaa ccccaaggtc accgagcgcg gctacctgga
     1561 agtcaaccgc atctgcggcc gcgacgacgt gcagggggcc gtcggcgccc agttcgcgaa
     1621 gagcaagaac gtcaagagcg cctacgtcct ccacgacaag acggcctacg gccagggcat
     1681 cgccgagtac ttcaagcgcg aggccgagac cctcggcatc aaggtcatgg gcttcgccgg
     1741 caccgaggaa aaggccaact tcgacgccat cctcaccccg atcctcgcct ccaagcccga
     1801 ggtgatctac ttcggcggca tgtacgacca gagctcggtc ctcttcaagc aggcgcgcca
     1861 gaagggctac aagggcatgt tcctgtcgga cgacggcttc gactcctccg acgcggccaa
     1921 gatcggcggc gacgccctcc tccagggcgc cggcacctac tactccaccg tctccggccc
     1981 ggcgagcgtc tatcccggca ccgccaagtt catcgcggac ttcaaggcga agttcggcgc
     2041 cgacccccag cccttcgcgg cccagggcta cgactgcgcg ggcatcgccc tcaaggcgat
     2101 cgagaacgcg atcctcgcca acggcaagaa gctgcccacc agggcggcgg tggccaacgc
     2161 cgtgcgcgcc ctcaaggact accagggcat cacgggcgtc atcaacttca acgccaaggg
     2221 cgacctcacc aaggccaagt acttcatcat ccaggtcacc tcgaacgatc cggccaagtg
     2281 gcccatgaac aagatcgacc agaccctgga catcgctccc ccgcagtgat cccacagcca
     2341 cagacgtgaa acctccccgc cccatccggg gcggggacat ccgggaggcc ccatgcgaaa
     2401 attcctgggt tcggaagcgt tgcgcgaact gatcttgccg ctcgccagga tggctgtctt
     2461 cgtcctgggc gcgggcgccc tgttctccgc catcatggtc gtcctcgccc tgctcgcgag
     2521 gggctccgga gccgaccagc tcctcctttc cacgcagaac acaaatatcc ttgcatatac
     2581 cctgggcaac ctgccccagg tcctcatcga cggggtgacc ctcggcttcg tgtacgcggc
     2641 catcgccctc ggctacacga tggtctacgg cgtcctcgag ttcatcaact tcgcccactc
     2701 cgagatcttc gcgatcgggg ccttcaccgg caccgagttc ctcatcacga tggacaggct
     2761 cggctacctc aagtcggcga gccccacggc ggcctacgtc tacctcgcga tctccctcct
     2821 catcggcatg atggcggcgg gcggggtcgc ggtgctgatg gagaggaccg cctacaggcc
     2881 gctgcgcgac tcgcccaggc tcgtccccct catctcggcc atcggcgtct ccttcgccct
     2941 ccaggacgtc atccgcctgg tcgagagcct caccacgggg cagttctacc gcgtcatgcc
     3001 caccttcggg agcttcgacg accgcctgcc cctcttctca atcgccgggg tcgacgtcga
     3061 catccaggtc aagtcggtcg tggtcatcgg cgcggccgtg atcatgctcg tgggcctcaa
     3121 ctacatcgtc aacgcgacca gggtcggcaa ggcgatccgc tcggtggccc aggaccgcaa
     3181 cacggcggcc ctcatgggca tcggggtcaa cggcatcatc tcgctcacct tcctcatcgg
     3241 cggtgccctg ggcggggcgg cgggcgtcct gtactcgctc aagttcaccc gcatcgaccc
     3301 cttcgtgggc ttcatgccgg gcatcaaggc cttcgcggcc gccgtcctcg gcggcatcgg
     3361 caacctcacg ggggccctcc tcggaggcat catcctcggc atgctcgaga gcttcgcggg
     3421 cgcctatctc ggcaccttca cgatggggaa cttcggggcg gagtacaagg acatgttcgc
     3481 cttcctcgtc ctcatcctcg tcatcatttt ccgccccacg ggtctgctcg gcgagcaggt
     3541 ctcgcagaag gcctaggagg atcgcgatgc gcttcaatct tcgtgaggcc ctccgggact
     3601 tctggagcct catcaaccgg ggcaagaacc cctacaccct ctccgtcggc ctcctcgtcg
     3661 tggctgccgt cctcgtctac ctgagcccga ggtcgatccc cgtcttcctc ctcttcgcgg
     3721 tagcggccgg cctgccctac ttcctcgaga tgcgcaagcc cgccaagctc gccatcgcgg
     3781 tct
//
LOCUS       DASSQV010000139         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_780711, whole genome shotgun sequence.
ACCESSION   DASSQV010000139 DASSQV010000000
VERSION     DASSQV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780711"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFL04_02410"
     CDS             <1..298
                     /locus_tag="VFL04_02410"
                     /inference="COORDINATES: protein motif:HMM:NF021295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838585.1"
                     /translation="VRESFPPRVGRVMGAAFRLMPSAFYLSELATDRSHMRRADGSWL
                     APPPPWDAPGAGRPGNLLSWIDPRLGVPGRVLDLEGFLGMAAPGQMEGRDARPS"
     gene            282..1823
                     /locus_tag="VFL04_02415"
     CDS             282..1823
                     /locus_tag="VFL04_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET7838586.1"
                     /translation="MRGRAEGGAPGKMSTPDSRAAGAMLGLAIGDALGLPVDFGSRAE
                     RLADPVTGLRAAAAWGGGPGSWSGETSLALRQAESILASGFDPGDFGQRALAWLDAGG
                     RGAGPIALGGAVGRSLGRIRGGLPAVLSGGRGENDNGNAALVRILPASIWLAAMPDPL
                     RWRALAAFSALTHGHPRCFLAAWIHSLVASGLLGGSSPADAYAAAVDAARAALPQMHH
                     ALRAEAEHFGRILGGKLAALPASDLRGSGYVVHNLEAGLWCLLTGADFGACVLSAVNL
                     GENAKASAATAGGLAGLAHGREGLPRDWVAQLDKAEEIEATGLALAALVEAPTPVPGS
                     YRILPGKLFAGPHPLAPQRGVEGDSTETRLSAILDSGIDGILDLTEDGETLGGRAVPQ
                     YWKEYETLAARRGMGVERRRLSIADMSTAGGGVIGRALGEIDAMLAAGRRVYVHCLGG
                     TGRTGTIVGAWLIERGLASPSEAIGLLAALRSASGAPDRASPETDGQRAAVIGWRPGP
                     SALTV"
     gene            1832..>2720
                     /locus_tag="VFL04_02420"
     CDS             1832..>2720
                     /locus_tag="VFL04_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HET7838587.1"
                     /translation="MPRVAIVSIGVEGSSETLPLGAATVVAALRAEPRLAGRLEAFIL
                     EGEEGGTAAGLAEALRKAGADWVGFSLYSWNRGIALEAARRLRAAFPGMLLFAGGPEA
                     TADPARVLSEGSLDFVVAGEGESACADLLARLLDSPKPREAVARLGALAGIETPTAPA
                     GGRRTAVEDPAGLASPWLAGMRGRGPEPVLDPRGRESLLWELARGCPFRCAYCYESKG
                     ESGVRRFPMDRIEAELELFVRAQAGQVFVLDPTFNADRERAARLLALFARKAPGVRWK
                     FEIRAELLDRRLARAFSALD"
BASE COUNT          332 a          993 c          992 g          403 t
ORIGIN      
        1 cgtccgggag agctttcccc cgcgggtggg gcgcgtcatg ggggccgcct ttaggctcat
       61 gccttcggcc ttctacctct ccgagctcgc caccgacagg tcgcacatga ggcgggccga
      121 tggctcctgg ttggcccccc ctccgccatg ggatgcccca ggcgccggaa ggcccggcaa
      181 cctcttgtcc tggatcgatc ccaggctcgg cgtccccggc cgggtcctgg acctcgaggg
      241 cttcctcggg atggcggccc cggggcagat ggagggacgc gatgcgcggc cgagctgagg
      301 gcggggctcc cgggaagatg tcgacccccg attcgcgggc ggcgggagcc atgctgggac
      361 tcgccatcgg cgatgccctt ggccttcccg tggatttcgg atcgagggca gagcggttgg
      421 ccgatcccgt gaccggcctc agggccgccg cggcgtgggg cgggggcccg ggctcttggt
      481 cgggcgagac ctccctcgcc ctccgccaag ccgaaagcat cctggcctcg ggcttcgacc
      541 ccggagattt cggccaacgc gccctggcct ggctcgatgc gggcgggcga ggcgcggggc
      601 ccatcgccct cggcggggcc gtcgggcgct ccctcggacg catccgcggc ggtctgccgg
      661 ccgtcctctc cggcggcagg ggcgagaacg acaacggcaa cgccgccctt gtccggatcc
      721 tgccggcctc gatctggctc gccgcgatgc ccgatcccct gcggtggcgg gccttggccg
      781 ccttctcggc cctgacccat ggccacccgc gctgcttcct ggccgcctgg atccattccc
      841 tcgtcgccag tggcctcttg ggtggatcca gccccgccga tgcctacgcc gcggccgtgg
      901 atgcggccag ggccgccctc ccccagatgc accatgccct tcgggccgag gcggagcatt
      961 tcggccggat cctcggaggc aagctcgccg ccctgccggc ctcggacctg cgcggctcgg
     1021 gctatgtcgt ccataacctc gaggcggggc tctggtgcct cctgaccggt gcggacttcg
     1081 gggcctgcgt gctgagcgct gtgaaccttg gcgagaacgc gaaggcgagc gcagcgacgg
     1141 ccgggggtct ggccggcctg gcccacggca gggagggcct gccccgggac tgggtcgcgc
     1201 agctcgacaa ggccgaggag atcgaggcca caggcctggc ccttgcggcc ctggtcgagg
     1261 cgcccactcc ggtccccggc tcctacagga tcctcccggg caagctcttc gccggccccc
     1321 atcccctcgc cccgcagcgg ggagtcgagg gcgactccac cgagacaagg ctttcggcca
     1381 tcctcgattc ggggatagac gggatcctgg atctgaccga ggacggggag accctcgggg
     1441 gcagggccgt gccgcaatac tggaaggaat acgagaccct cgcggcccga cgcggaatgg
     1501 gcgtcgagcg gagacggctg tccatcgccg acatgagcac ggcggggggc ggggtcattg
     1561 gccgggccct tggcgagatt gatgcgatgc tcgccgcggg ccgccgcgtc tacgtgcact
     1621 gcctcggcgg aacggggagg acggggacca tcgtcggcgc ctggctcatc gagcggggcc
     1681 tggcctcgcc ctctgaggcc attggcctcc tcgccgcgct caggtcggcc tcgggggctc
     1741 cggatcgggc ctcacccgag accgatgggc agagggccgc cgtcatcggc tggcggccgg
     1801 gtccctcggc cctcacggtc taggcagagc catgccgcgc gtcgccatcg tatccatcgg
     1861 cgtcgagggc tcctccgaaa cccttcccct cggcgcggcc accgtagtcg ccgccctcag
     1921 ggccgagccc aggctcgccg gccgcctcga ggctttcatc cttgaggggg aggagggcgg
     1981 aacggcggcc ggcctcgccg aggcgctgcg gaaggccgga gccgactggg tgggcttctc
     2041 gctgtactcg tggaaccgcg ggatcgccct cgaggcggcg aggcgcctcc gtgccgcctt
     2101 tcccgggatg ctcctcttcg ccggcgggcc cgaggccacg gcggacccgg cccgggtcct
     2161 gtctgagggc agcctggact tcgtcgtggc gggggagggg gagagcgcct gcgccgatct
     2221 ccttgcgcgg ctcctcgatt cccccaagcc ccgagaggcc gtcgcgcggc tcggcgcgtt
     2281 agccggcata gagaccccga ccgccccggc cggaggcagg aggacggccg tggaggatcc
     2341 cgccgggctg gcctctccct ggctggcggg aatgcgggga agagggccgg agccggtcct
     2401 cgatcccagg ggccgagaga gcctgctctg ggagctcgcc cgcggctgcc ccttccgctg
     2461 cgcctactgc tacgagtcca agggcgagtc gggagtcagg cgcttcccca tggaccgcat
     2521 cgaggccgag ctcgagctct tcgtccgggc ccaggcgggc caggtcttcg tcctcgaccc
     2581 caccttcaac gccgacaggg aaagggcagc caggctcctt gccctattcg cgaggaaggc
     2641 ccccggcgtc aggtggaagt tcgagatcag ggccgaactg ctcgaccggc gtctcgcccg
     2701 cgccttctcc gccctcgact
//
LOCUS       DASSQV010000140         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1650744, whole genome shotgun sequence.
ACCESSION   DASSQV010000140 DASSQV010000000
VERSION     DASSQV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650744"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1302)
                     /locus_tag="VFL04_02425"
     CDS             complement(187..1302)
                     /locus_tag="VFL04_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838588.1"
                     /translation="MKPRVTLLVLALGAAGASAQDAGSLSLNGSLSAQPYAAWSSASQ
                     STGAPGAGASFSYGSTTSLGLELAAKGQSARAAASIEAAVLTGQAAADAWAIANGPFA
                     RADELLLPAPQTSGPAPETLVAARIRSLYVKVDGARASLIAGRQVINYGRGALWNPAD
                     IFTELDLTGLSPVRRGSDALRLLVPLGPTEGLDLVAAPLARPAEGRYAIRASGVAGSL
                     DGALIAARDGQRGARVAGADFKTDLELGVVGDAELALLDSGRRELRAAGGLDYSIGEI
                     VMAVEYYYNGGGAAADPLFPASHNAYASATWEASQLASASAVAIVDLGSASGSLTLLG
                     DLSLAQNAELELFAKGTRSGGPAVYGVQAGFSLAVKF"
     gene            complement(1311..2054)
                     /locus_tag="VFL04_02430"
     CDS             complement(1311..2054)
                     /locus_tag="VFL04_02430"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HET7838589.1"
                     /translation="MRRHAHALAACILGWACAGAAAAQTADEIIARVDANMSWGSIRY
                     SARMEITVGGETRYKTMDAVSQGSTKAFAEFTNPEDKGTRYLKLDKNLWIYFPKEQDT
                     VKISGHLLKEGMMGSDVSYEDSLESSDFRARYTASLKGKEEVDGRPCFVLELAAKVPT
                     AAYDRRLLWIDAERFVGLRQEMYAKSGKLLKTSRVLELTRLGERWFPSRSEYVSKLRN
                     NTKTVFSMADIQLDPKLDERQFSMATLTK"
     gene            complement(2083..>2607)
                     /locus_tag="VFL04_02435"
     CDS             complement(2083..>2607)
                     /locus_tag="VFL04_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7838590.1"
                     /translation="ALASAQGPGGGQRLSVQSWTSIGDIARIASMTATIYFWIELVIA
                     FLGAFIIANIMMMVVLERRREIGIMKSMGMENGRILAQFLAEGTMLGLIGSAAAAVCG
                     TALNAFLAARGIDFSKTVSGTNLPMDNIIHPGVHPLNVLALFGLGVLISAIVAYLPSR
                     SAATMDPIEAIRSV"
BASE COUNT          354 a          888 c          957 g          408 t
ORIGIN      
        1 gacttcgccg ggctcggcgg aggctcggcg ggggaggccc gcggcgtcat cacccggggc
       61 ctcgccgtcg tgcccgcggg tggcccggcc ctgcgcgccg aggccctggc gagctacgtc
      121 atcatggagg ccgccaggtg aggccgagcc cgccgagggc ccggcgggtc gagggccggc
      181 ccgcccttag aacttcacag cgaggctgaa gccggcctgg acgccgtaga cggccgggcc
      241 cccgctccgc gtgcccttcg cgaagagctc gagctcggcg ttctgggcga ggctcaggtc
      301 gccgaggagg gtgaggctgc ccgaggccga gcccaggtcg acgatggcca cggccgaggc
      361 cgaggccagc tggctcgcct cccaggtggc gctcgcgtag gcgttgtgcg acgccggaaa
      421 gagggggtcg gccgcggccc ctcccccgtt gtagtagtac tcgaccgcca tgacgatctc
      481 cccgatggag tagtcaaggc cgcccgcggc cctgagctcg cggcgccccg agtcgaggag
      541 ggcgagctcg gcgtccccga cgacgcccag ctcgaggtcg gtcttgaagt cggcccccgc
      601 gaccctggcc ccgcgctggc cgtcccgggc cgcgatgagg gccccgtcga ggctccccgc
      661 caccccgctg gccctgatgg cgtagcggcc ctcggcgggc ctggccaggg gcgcggccac
      721 gaggtcgagg ccctcggtcg gcccgagggg gacgaggagg cgcagggcgt cgctgcccct
      781 ccggaccggc gacaggcccg tgaggtcgag ctcggtgaag atgtcggcag ggttccagag
      841 cgcgccccgg ccgtagttga tcacctggcg tcccgcgatg agggaggccc gggcgccgtc
      901 gaccttgacg tagaggctgc ggatccgggc ggcgaccagg gtctcgggcg ccggccccga
      961 cgtctgggga gccgggagca ggagctcgtc ggccctcgcg aagggcccgt tggcgatcgc
     1021 ccacgcgtcg gcggcggcct ggcccgtgag gacggcggcc tcgatggagg cggccgccct
     1081 cgcgctctgg cccttcgccg cgagctcgag gcccaggctc gtcgtcgagc cgtaggagaa
     1141 gctggccccg gcccccgggg cgcccgttga ctggctggcc gagctccagg ccgcgtaggg
     1201 ctgggccgag agcgagccgt tgaggctcag tgagcccgcg tcctgggccg aggcccccgc
     1261 cgctccgagc gcgaggacga ggagtgtcac tcgtggcttc atgcccggcc ctatttggtg
     1321 agggtcgcca tggagaactg ccgctcgtcg agcttggggt cgagctggat gtcggccatc
     1381 gagaagacgg tcttggtgtt gttccggagc ttcgacacgt actcggagcg cgaggggaac
     1441 cagcgctcgc cgagccgcgt gagctcgagg accctgctcg tcttcagcag cttgcccgac
     1501 ttagcgtaca tctcctgcct gaggcccacg aagcgctcgg cgtcgatcca cagcagcctc
     1561 ctgtcgtagg cggccgtcgg gaccttggcg gcgagctcca ggacgaagca gggccgtccg
     1621 tcgacctcct ccttgccctt gagcgaggcc gtgtaccggg cgcggaagtc cgaggactcg
     1681 aggctgtcct cgtaggacac gtcggagccc atcatcccct ccttgaggag gtggcccgat
     1741 atcttcacgg tgtcctgctc cttggggaag tagatccaga ggttcttgtc gagcttgagg
     1801 tagcgggtcc ccttgtcctc ggggttcgtg aactcggcga aggccttggt cgagccctgg
     1861 ctcacggcgt ccatcgtctt gtaccttgtc tctcccccca cggttatctc catgcgggcg
     1921 ctgtagcgta tgctccccca ggacatgttg gcgtcgaccc tggcgatgat ctcgtcggcg
     1981 gtctgggccg ccgcggcccc cgcgcaggcc cagccgagga tgcaggccgc gagggcatgg
     2041 gcgtgtcttc tcatatcggt cctccctatg gtgcggcggt ccctagaccg accttatggc
     2101 ttctatgggg tccatcgtgg ccgcgctccg cgagggcaga taggccacga tggccgaaat
     2161 caggacgccc aggccgaaga gggccaggac gttgaggggg tggacccccg ggtggatgat
     2221 gttgtccatg gggaggttcg tgcccgagac ggtcttcgag aagtcgatcc ccctcgcggc
     2281 gaggaaggcg ttgagggccg tcccgcacac cgccgccgcc gccgagccga tgaggccgag
     2341 catggtcccc tcggcgagga actgggcgag gatcctcccg ttctccatgc ccatggactt
     2401 catgatgccg atctccctcc gcctctcgag aaccaccatc atcatgatgt tggcgatgat
     2461 gaaggccccg aggaaggcta tcacgagctc gatccagaag tagatggtgg ccgtcatgga
     2521 cgcgatgcgc gcgatgtcgc cgatcgaggt ccagctctgg accgagagcc tctgtccgcc
     2581 gcctgggccc tgggccgagg cgagggc
//
LOCUS       DASSQV010000141         3410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2399019, whole genome shotgun sequence.
ACCESSION   DASSQV010000141 DASSQV010000000
VERSION     DASSQV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2399019"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1321)
                     /locus_tag="VFL04_02440"
     CDS             complement(107..1321)
                     /locus_tag="VFL04_02440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7838591.1"
                     /translation="MAVRIERVEREFILVSAAEARTEARLQAAGRTLRCRIVSVGDGR
                     LALAHTASDFAQLKARQRVSVYFDFRGQGVSFTAPVLNSGPESIDLGPPEGMYRSLSR
                     RWPRVPPPSGLAVDFILPDAELKLDCPESVEWAEVELPELQQGLETGDLSSLVASFKA
                     KAETLAGEGRVIMFKDRSPLDAAQEMAAKLGRVLFVPSTLGGLPQVDPYPRGRIITRD
                     MAEDFEGLSALVEGSPLGSYLAGRAAGGLASAVWCPVVYYRYTVGMVELSNGTGRPKS
                     LGLTAVDLAWEFARVLAWFLRRHGYFGSARETEGVRRGSIIDASPSGLLVELPAKGPR
                     FVDGSLIKIRLSAGDWSLACAARVARRYGDGNTDFYGLAFQALMPDEVAGIARGLYGE
                     EEAVPLEARGRG"
     gene            1456..3057
                     /locus_tag="VFL04_02445"
     CDS             1456..3057
                     /locus_tag="VFL04_02445"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET7838592.1"
                     /translation="MTRYDDLDRAPAWDKLRSLGRPRLGELLSPARIASSSIPMGGGL
                     SYNWAASPVDEAVLALLGRLAEEQELVAKYRLLLDGEVMNTGEQRMVLHHLARGQLGR
                     TVSHEGRDQRQFYEGERLKAADFARSVHGGRIRGSSGKAFSTVTQIGIGGSDLGPRAL
                     YLALERWARANGKLRMEARFISNVDPDDANAVLERLDLETSLFVLVSKSGTTQETLAN
                     ESLLRERLRARGLDPAKHIVAVTSETSPLARNPGYLASFYIDDFIGGRYSSSSMVGGV
                     VLSLALGSEAFADFLAGAHAADRAALEGDISRNAALLDAMIGVYERSVLGYPCTVVLP
                     YAEALSRFPAHLQQLDMESNGKRVNRRGEALSYSTGPVVFGEPGTNGQHSFYQLLHQG
                     TDPLPLQFIGFLEGQTTGDCLVEGSTSRQKLNANLAAQIVAFARGKADANGNRHFPGE
                     RPSSLILGRQLEPASLGALLAHYENKVMFQGFAWNINSFDQEGVQLGKVLAKKALTGL
                     AEGAAPASGDPVLDAYAKLLLGGRA"
     gene            3065..>3410
                     /locus_tag="VFL04_02450"
     CDS             3065..>3410
                     /locus_tag="VFL04_02450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7838593.1"
                     /translation="MADRNVIAFTGGGSGGHVFPGLAVIERLREKGFQGRIVWLGSRK
                     ESDRAAVEAAGVEYRPIPSGKLRRNLSLENAADAFRVLAGYLASRAILAELRPCLLFS
                     KGGYVSVPPCAAA"
BASE COUNT          547 a         1229 c         1151 g          483 t
ORIGIN      
        1 ccgatgtagc gcagggccgc ccagaccacg atggtcccga gggcgacggc gatgaccctc
       61 cagtccgaga gcacggcgag ggagagcttc atgtagccgt cgacgctcat cccctccccc
      121 tcgcctcgag cggcaccgcc tcctcctcgc catagaggcc gcgggctatc cccgccacct
      181 catcgggcat gagggcctgg aaggcgaggc catagaaatc ggtgttcccg tccccgtagc
      241 gccgtgccac cctggcggcg caggcgaggc tccagtcgcc cgcggacagg cggatcttga
      301 tgagcgagcc gtcgacgaag cgcggcccct tggcggggag ctcgacaagg aggcctgagg
      361 ggctcgcgtc gatgatggag cctcggcgca cgccctcggt ctcgcgggcc gagccgaaat
      421 agccgtggcg cctgaggaac caggcgagga cgcgggcgaa ctcccaggcg aggtcgacgg
      481 cagtgaggcc gagggacttg ggcctccccg tcccgttcga gagctccacc atgcccacgg
      541 tgtagcggta gtagacgacg gggcaccaga cggccgaggc gaggcccccg gccgctcgcc
      601 ctgcgaggta ggagcccagg ggcgagccct cgaccagggc cgacaggccc tcgaagtcct
      661 cggccatgtc gcgggtgatg atccggcccc ggggataggg atcgacctgg ggaaggccgc
      721 ccagggtgga ggggacgaag aggacccggc ccagcttggc ggccatctcc tgggccgcgt
      781 cgaggggcga tctgtccttg aacatgatca cccttccttc gccggcgagg gtctcggcct
      841 tggccttgaa gctcgcgacc agggaggaga ggtcaccggt ctcgaggccc tgctggagct
      901 cggggagctc gacctcggcc cattcgaccg attcggggca atcgagtttg agctcggcgt
      961 cgggcaggat gaaatcgacg gcgaggcccg agggcggcgg gacccggggc cagcgccggg
     1021 acaggctccg gtacatgccc tcggggggcc caaggtcgat ggattcgggc ccggagttga
     1081 ggacgggggc ggtgaaggac acgccttggc cacggaaatc gaaatagacc gagacccgct
     1141 gtctggcctt gagctgggcg aagtccgagg ctgtgtgggc gagggccaga cggccgtctc
     1201 cgaccgacac gatgcggcag cggagggtgc ggcccgccgc ctggagcctc gcctcggtcc
     1261 tcgcctcggc cgccgatacc aggatgaatt cgcgctcgac cctttcgatt cgtactgcca
     1321 cgcctgcctc cggagggtat cttagcacat tcccgcagcc cgccacgcct tgatgctggc
     1381 gacctcgggc tatatcatga tgcatgacga ggccggatgc acgtccggcg cgaaaacggt
     1441 cgaaggagcc gagcaatgac gagatacgac gacctcgatc gggccccagc ctgggacaag
     1501 ctcaggtccc tcggccggcc cagactcggc gagctcctca gcccggcgcg gatcgcctcc
     1561 tcctcaatcc ccatgggagg gggcctcagc tacaactggg cggcctcgcc cgtcgacgag
     1621 gcggtcctgg ccctcctcgg ccggctcgcc gaggagcagg agcttgtggc gaaataccgc
     1681 ctcctcctcg acggcgaggt catgaacacg ggcgaacaga ggatggtcct ccatcacctc
     1741 gcccgagggc agctgggcag gaccgtgagc cacgagggca gggaccagcg gcagttctac
     1801 gagggcgagc ggctgaaggc cgcggatttc gcccgctcgg tgcacggcgg caggatcagg
     1861 ggctcgagcg gcaaggcctt ctcgaccgtc acccagatcg ggatcggcgg ctccgacctc
     1921 ggtccgcggg ccctctatct ggccctcgag cgctgggcca gggccaacgg caagctccgg
     1981 atggaggccc gcttcatctc gaacgtcgac cccgacgacg ccaacgccgt cctcgagcgg
     2041 ctcgacctcg agaccagcct cttcgtcctc gtgtccaaga gcgggaccac ccaggagacc
     2101 ctcgccaacg agtccctgct ccgcgagcgg ctccgggccc ggggcctcga tccggccaag
     2161 cacatcgtcg cggtgacgag cgagacgagc cccctcgcca ggaaccccgg ctatctcgcc
     2221 tccttctaca tcgacgactt cataggcggg cgctactcct cctcgagcat ggtgggcggg
     2281 gtcgtgctct cgctcgccct cggctctgag gccttcgccg acttcctggc cggggcccac
     2341 gccgccgacc gcgccgccct cgagggcgac atatcgcgca acgcggccct cctcgacgcg
     2401 atgatcgggg tctacgagcg gagcgtcctc ggctacccct gcaccgtcgt cctgccctac
     2461 gccgaggccc tctcgcgttt ccccgcccac ctccagcagc tcgacatgga gtccaacggc
     2521 aaacgcgtga accgcagggg cgaggccctc tcctattcca ctggccccgt ggtgttcggc
     2581 gagccgggca cgaacggcca gcactccttc taccagctcc tccaccaggg caccgatccc
     2641 ctgcccctcc agttcatcgg cttcctcgag ggccagacca cgggcgactg cctcgtcgag
     2701 ggctcgacca gccggcagaa gctcaacgcc aacctcgccg cccagatcgt ggccttcgcc
     2761 cgaggcaagg ccgacgcgaa cggcaacagg cacttccccg gcgagaggcc gagcagcctg
     2821 atcctcggca ggcagctcga gccggcctcg ctcggggccc tcctcgccca ctacgagaac
     2881 aaggtcatgt tccagggctt cgcctggaac atcaactcct tcgaccagga gggggtccag
     2941 ctcggcaagg tcctcgccaa gaaggccctc acgggcctgg ccgagggagc cgcccccgcg
     3001 agcggcgacc ccgtcctcga cgcctacgcg aagctgctgc tcggcggcag ggcctgaggc
     3061 tgcggtggcg gacagaaacg tcatcgcgtt cacgggcgga ggctcgggag gccatgtctt
     3121 ccccggcctg gccgtcatcg agcgcctcag ggagaagggc ttccagggcc gcatcgtctg
     3181 gctcggctca agaaaggagt cggaccgcgc ggccgtcgag gctgcgggcg tggagtacag
     3241 gccgatcccc tctggcaagc tccgcaggaa cctctccctt gagaacgccg ccgacgcctt
     3301 ccgcgtcctc gccggctacc tcgcctcccg cgcgatcctc gccgagctga ggccctgcct
     3361 cctcttctcg aaggggggct acgtgagcgt cccgccctgc gcagctgccg
//
LOCUS       DASSQV010000142         8048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_906496, whole genome shotgun sequence.
ACCESSION   DASSQV010000142 DASSQV010000000
VERSION     DASSQV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906496"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1239)
                     /locus_tag="VFL04_02455"
     CDS             complement(148..1239)
                     /locus_tag="VFL04_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HET7838594.1"
                     /translation="MKSARGLVMGPAPYVFSRQSTATWTWGTALALVPAFIWGLYCFG
                     AGAALPVVASVLAALVGEAGVGLFRKRITLGDGSAALTGFLVGLAMPPSIPLYIPAAS
                     ALFAIIMIKGIFGGIGSNWMNPALGGIVFALLNWPRQMGEWVVPRHLAGVVGVSGATP
                     LGFVRDRLGAAPAGSDPLSLLAAAGMKFSDLDRSIREALNFLLFSRIGADLPAGYIDL
                     LIGNRPGALGEISGILILAASVFLLSRRIIRWEIPASVMFSYALIVWVFGGLPYGNGF
                     FAGDVLFAILSGSFLIVTFFMAPDPVTSPSTRTGMLAYGFGIAVLTFLIRSYGAMSEG
                     TAFAVLTMNCLVPLITGGRAARPARLGRD"
     gene            complement(1236..2543)
                     /locus_tag="VFL04_02460"
     CDS             complement(1236..2543)
                     /locus_tag="VFL04_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HET7838595.1"
                     /translation="MKSIRTFRKGGIEVAFGTAWSDTGPVPSAFLPNEAAVLLSQHHG
                     PAARCVVHKGEFVREGAVIGRARGPESSNVHAPIPGIVKDIRTVPLSSGGECEAVIIA
                     LEGSFDRLGRRPERYLWKTMSRHDILHSLEERGVIETEAPGVPINALIGESRPIDLLI
                     LNAVESEPYLNTETALVRDKWAEILVGLAIMRKVVAPARTVVALQAGKAPGVALPTDP
                     EEIPPELIELRPRYPQDLRNQLLDAITDHRAKPGQELLIVRPSTAFAVFEAIVHVKPM
                     LERYITVAGGAVKRPSVLKARLGTSIGDLIEECGGFIGRPARLIVGGPFRGRAVHNLD
                     VPVTKTTSAVLALTDSEIGRQSRCACIRCGRCSEACPERLDPDLLFRLLTHGREGEAL
                     EHRLESCTLCGICGHLCPSRVPLVAAFAARLSALDGRTKGARP"
     gene            complement(2540..4111)
                     /locus_tag="VFL04_02465"
     CDS             complement(2540..4111)
                     /locus_tag="VFL04_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838596.1"
                     /translation="MRSTEPGRHRAYACMGIAAAGLGVLLSFSSYMQAPVEAHTEAWR
                     GYRSLLVEESVPDSEVMDALSKAGVTGVISASTEPVLISNWSGLEQTTLSLALARLIP
                     GDPRRDPYIDGLGSWFRARVQGRGYRVYYLRVSPFSEALRAGDSALAPFSGRFILPES
                     GATVSGSAPQRWMAMGAVGIVLLYVLVSALGPSRRLGPKVLRRFLLRLCLVLPWLALA
                     WRGTTELILSLLWTVALVELSASLDIPLEELRRNHAIGSTLKSLLRGGRPPLSALLVA
                     SGALVLAPACLLVTALAILSSAGFLIAFCCASLLESRLAKRGRLEFVPIAIGRRGRTR
                     DYASAGICILALVAWAAFRIVPVGSGESQAAGIEMPMPLPGRGNPAPSLEEARAQANS
                     SQAMPGLAQWLVHRAFQEALPLARLKEGRSDPFAQLSLGKGGATGPSIVFNESWRTSA
                     LRGIPPNSIEAMLLAQGGSVRGASLPWQPTGSGPLAPIEALLYIFLLVPPIRRIAAGA
                     GAARSSPSGEIRQAA"
     gene            complement(4123..4602)
                     /locus_tag="VFL04_02470"
     CDS             complement(4123..4602)
                     /locus_tag="VFL04_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HET7838597.1"
                     /translation="MNPPARLESIDLLHNPVFLSAIFSLFIAQFIKVLINILRPRAKG
                     FRDVLVTFLWRTGGMPSSHSALAVSIATSVAFVEGPNSSVCILAIFFALVVIRDAMGV
                     RRAAGQQARVLNLISKELSSRFDLNFHQVKEVHGHSAAEVLVGSILGFSIALAFCTL"
     gene            4714..5712
                     /locus_tag="VFL04_02475"
     CDS             4714..5712
                     /locus_tag="VFL04_02475"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7838598.1"
                     /translation="MPPRKEDLTRRAGADDAGRRLDKVLRRMFPSLGLSDIYGRLRKR
                     TIRVNGARAEPACRLAEGDLISFPAELLTGGEDSTPPSEPIGSRNGSGLGGILVLETR
                     DLLFLNKPRGLLSHGPGGLDELVEAAMRDRIGMSLSFRPGPLHRLDRNTSGLITFPRT
                     AEGSRAFSMMLRSRRIRKRYLALLDGVLDRELLWEDRLVRDDRTRTSHAGAEAERSPD
                     EAARGRRAVSRIVPLIGSQGLCLAMIEIQTGLTHQIRAQASLHGRPLAGDRKYGGSRH
                     ASGFLLHAHSMEFSEPPFDDVPRRVIASLPSEASERLASIFGPNEVEKAQSLALMS"
     gene            5821..6759
                     /gene="hflK"
                     /locus_tag="VFL04_02480"
     CDS             5821..6759
                     /gene="hflK"
                     /locus_tag="VFL04_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HET7838599.1"
                     /translation="MVIIGIAAVLVLIFLSTSFFIVDQTSRAVITTFGKYTREAEPGL
                     HYKIPFGIQRSYNVPTEVIQTEQFGFRTEKAGVVTQYSETSYPQESVMLTGDLNIVDV
                     EWIIQYRIVDPRAWLFNTSDRHKTIRDISQSVVNLLVGDRTILGVIGPERTTIEDSAV
                     QMMNETFNNYGLGIRVTQVKLQNIVPPKGVQDAFEDVNKAIQDMNRLINEGKEAYNSE
                     IPKAQGQAQQAVEVAQGYATERVNQAKGDVARFTSVYDEYRKAPGITRQRLYYEMMEA
                     VFKGEKDVELIDKRFSNFLPLKNLESKPAATQGAGQ"
     gene            6756..7736
                     /gene="hflC"
                     /locus_tag="VFL04_02485"
     CDS             6756..7736
                     /gene="hflC"
                     /locus_tag="VFL04_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HET7838600.1"
                     /translation="MKRLIIILVVIAIVAIAFFATGPFYVLNEGEQAVVVRLGKIVGV
                     EKDAGLKLRSPFLDVIVKYPKQVMPWDGEPQRIPTKENQFIWVDTTARWKITDPVKFY
                     ESVSSLENAYGRLDDVIDNGVRTVISVNYLREAVRNSNNLLGTGTKESFQTGDVEGSA
                     ALEQLTQSNQSYETIDKGRRRLSEEMLAIVRPIVPQYGIEVIDIVPRQIKYSDELTES
                     VYNRMIKERNQIAQAFRSYGEGKKAEWLGKLENEKRSILSGAYQKAETTKGKADAEAT
                     RIYSSAYSQDPSFFDFWRAIESYRQTLPSFSKTLSTDLDYFRYLSSPQGR"
     gene            complement(7810..>8048)
                     /locus_tag="VFL04_02490"
     CDS             complement(7810..>8048)
                     /locus_tag="VFL04_02490"
                     /inference="COORDINATES: protein motif:HMM:NF025933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET7838601.1"
                     /translation="CGADLRRGTPLGSESLRAALDAFRPLLWVCGHIHESPSAQVVGG
                     TLVVNPGPLNEGRFARILVDRQSGAWSAKAELVS"
BASE COUNT         1544 a         2596 c         2705 g         1203 t
ORIGIN      
        1 ccgccaggcc ggcgatggcg ctccgctctc gcgccgatgc ggcggaatcg acaaggaggg
       61 cggcggtggc caggagcatg cccaggaaac ccatgatggc cgcggcggcg aggttctcga
      121 cgatggatat cttccgctca gccatggtca gtccctcccg agccttgcgg ggcgcgcggc
      181 gcgcccgcct gtgatgaggg gcacaaggca gttcatggtc aggacggcga aggcggtgcc
      241 ctcggacatc gcgccatagc tccggatgag gaaggtcagc acggcgatcc cgaagccata
      301 tgccagcatc cccgtccttg tcgaaggaga ggtcacggga tcgggagcca tgaagaaggt
      361 cacgatgagg aaggacccgc tgaggatggc gaagagcacg tcgcccgcga agaagccgtt
      421 gccgtagggc agaccaccga atacccacac gatgagggcg tagctgaaca tgacggacgc
      481 cgggatctcc cagcggatga tcctgcgcga gagcaggaag accgaggcgg cgaggatcag
      541 tatccccgag atctcgccaa gggcgcccgg cctgttgccg atcaggaggt cgatgtagcc
      601 cgcaggaagg tcggcgccga tcctcgagaa aagcaggaag ttgagggcct cccgtatcga
      661 tcgatccagg tccgagaact tcatcccggc ggccgcgagc aggctcagcg ggtccgagcc
      721 cgctggcgcg gccccaaggc ggtctcggac aaagccgagc ggcgtcgccc cgctcacccc
      781 gaccaccccc gcaagatgcc ggggcacgac ccattcgccc atctggcgcg gccagttcaa
      841 cagggcgaag acgatcccgc ccaaggcagg gttcatccag ttcgagccga tcccgccgaa
      901 gatccccttg atcatgatga tggcgaacag ggccgaagcc gcggggatgt agagggggat
      961 ggacgggggc atcgcaagcc cgacaaggaa tccggtcagc gccgcagagc catcgcccaa
     1021 ggtgatccgc ttccggaaga gtcccacacc agcctctccg acgagggccg cgaggacgga
     1081 ggcgaccacg ggcagggcgg cccccgcccc aaagcagtag agtccccaga tgaaggccgg
     1141 gacaagagct agggccgtgc cccaggtcca tgtcgctgtc gattggcggc tgaagacata
     1201 gggagccggt cccatcacaa gaccgcgcgc gcttttcatg gcctggctcc cttcgtccgg
     1261 ccgtcgaggg cggaaagtcg ggccgcgaat gccgcgacca ggggcaccct cgatgggcat
     1321 aggtggccgc agatcccgca cagggtgcag ctctccagcc gatgctccag ggcctccccc
     1381 tcgcgtccgt gggtcaggag gcggaacagg aggtcgggat caagcctttc gggacaggcc
     1441 tctgaacagc ggccgcatcg tatgcacgcg catcgggact ggcgcccgat ctcggagtcg
     1501 gtcagggcga gcacggccga ggtggtcttg gtgaccggga catcgaggtt gtgcaccgcc
     1561 cggccgcgga aggggccgcc gactatcagg cgggcgggac ggccgatgaa gcctccgcac
     1621 tcctcgatga ggtctcctat ggaggtgcca agcctcgcct tgagcacgga gggccgcttg
     1681 accgcccccc ccgcgaccgt gatgtagcgc tcgagcatcg gcttgacatg cacgatggcc
     1741 tcaaagacgg cgaaggcggt ggagggcctc acgatgagga gctcctggcc cggtttcgct
     1801 cggtggtcgg tgatggcatc caggagttga ttgcgcagat cctgcggata tcggggcctg
     1861 agctcgatga gctcgggagg gatctcctcc gggtccgtag ggagcgcgac gcccggagcc
     1921 ttgccggcct ggagggcgac cacggtccga gccggggcca cgaccttccg catgatcgcc
     1981 aggccgacaa ggatctccgc ccacttgtcc ctgaccaggg cggtctccgt gttgaggtag
     2041 ggctcgcttt ccacggcgtt gagtatgagg aggtctatcg gcctggattc gcctatcagc
     2101 gcgttaatgg gcacgccagg agcctcggtc tcgatgacgc ccctctcctc cagcgaatgg
     2161 aggatgtcgt ggcggctcat ggtcttccat aagtagcgct cggggcggcg cccaagcctg
     2221 tcaaaggagc cctcgagggc tatgatgaca gcctcgcact ctccgcccga cgaaaggggg
     2281 acggtcctga tgtccttcac gatccccgga atgggcgcat ggacgttcga ggactcgggc
     2341 ccgcgggcgc ggccgatcac cgcgccctcc cttacgaact cccccttatg gacgacgcag
     2401 cgcgccgcag ggccgtggtg ctggctgagc agcacggcgg cctcgttcgg caggaaggcg
     2461 ctcggcacgg ggcccgtgtc tgaccaggcg gtcccgaagg ccacttcgat gccgcccttc
     2521 ctgaaggttc tgatggattt catgctgcct gccgtatctc gcctgagggg gagctccgcg
     2581 cggcgcccgc gcccgcggcg atccgccgga tgggcggaac caagaggaat atatacagca
     2641 gggcctcgat cggcgcaagc ggtcccgatc ctgtgggctg ccaggggagg ctggcccctc
     2701 gtacggagcc gccctgcgcg aggagcatgg cttcgatgct gttcggcggg atgccccgca
     2761 aggccgaggt cctccacgac tcgttgaaga ctatcgaggg gcccgtggcc ccccccttcc
     2821 ccaggctcag ctgcgcgaag ggatcgctgc ggccctcctt gagcctggcc aggggcagag
     2881 cctcctgaaa cgccctgtgg acaagccact gggcaaggcc aggcatcgcc tggctggaat
     2941 tggcctgggc cctcgcctcc tcgaggctcg gcgccggatt tcccctgccc ggcagcggca
     3001 tcggcatctc gatccccgca gcttggcttt cgccgctccc gacggggacg atccggaacg
     3061 ccgcccatgc cacgagggct agaatgcaaa tgcccgccga ggcgtagtcc cgcgtcctgc
     3121 ccctccgccc gatcgcgatg ggcacgaact caagtcttcc cctcttggcc agccgggatt
     3181 ccaggaggga ggcgcagcag aacgcaatga ggaaaccggc cgaggacagg atcgcaagag
     3241 ccgtcacgag aaggcaggca ggggcaagga ccagggcgcc ggaggcgacg aggagggccg
     3301 aaagcggcgg ccgccctcct cggaggaggg acttcagggt cgagccgatg gcatggttac
     3361 gcctcagctc ctcgaggggt atgtcgaggg aggcagagag ctcgacgagg gcgacggtcc
     3421 agagcaggga caggatcagc tcggtagtgc ccctccacgc gagggccagc catggcagca
     3481 cgaggcagag ccgaaggagg aagcggcgga ggaccttcgg ccccaggcgg cgggatggcc
     3541 ccagggctga cacgaggacg tagagcagca cgatgcccac ggcccccatc gccatccagc
     3601 gctgcggcgc ggagccgctc acggtggcgc cggattcggg gaggatgaag cggcccgaga
     3661 aaggcgcaag ggcgctgtcg cccgcccgca gagcctcgga gaaaggcgag actcggaggt
     3721 aatagactcg gtagccgcgg ccctggaccc tggcccggaa ccacgagccc aggccatcga
     3781 tgtagggatc ccggcgggga tcaccgggga tcagcctcgc gagggccagg gagagggtgg
     3841 tctgctcgag gccgctccag ttggagatga ggacgggctc ggtggatgcc gagatgaccc
     3901 cggtaactcc cgccttggac agggcatcca tgacctcgga gtcagggacc gactcctcga
     3961 cgaggaggct ccggtagccc ctccaggcct ccgtatgggc ttctacgggg gcctgcatgt
     4021 aggatgagaa ggaaagcagg accccaagac cggcggcggc gatccccatg caggcgtatg
     4081 cccgatgcct gcccggctcc gtcgatctca cggcgccgct gcctataagg tgcagaatgc
     4141 gagggcgatc gagaaaccga ggatggaccc gacgaggact tcggccgcgc tgtggccatg
     4201 gacttccttg acctggtgga agttgaggtc aaagcgggac gagagctcct tgctgatgag
     4261 gttgagcacg cgggcctgct gtccggcggc gcggcggacc cccatcgcgt cgcggatcac
     4321 caccagggcg aagaagatgg ccaggatgca gacgctagag ttgggcccct cgacgaaggc
     4381 caccgaggtg gcgatcgaga cggccagggc gctgtggctc gacggcatcc cccccgtgcg
     4441 ccagaggaag gtgaccagca catcgcggaa acccttggcc ctgggcctga ggatgttgat
     4501 gagtaccttg atgaactgag cgatgaaaag actgaaaatg gccgagagga aaacgggatt
     4561 atgcagcaga tcgatgcttt ctagtctcgc cgggggattc accggcaaag tttcccccga
     4621 agcgactgcc cttgtcaagg cgcatcgctg agccgggggc cgggcctggc gcgccgattg
     4681 accccccgaa gcgtcggcat atagagtgtg ccgatgcctc cacgaaagga agacctgacg
     4741 aggcgggccg gagccgacga cgccgggcgg aggctcgaca aggtcctgcg cagaatgttc
     4801 ccctccctcg gtctgtcgga catatacggc aggctgcgga agaggaccat ccgcgtgaac
     4861 ggggcgcggg ccgagcccgc ctgccgtctg gccgaaggcg acctgatcag cttccccgcc
     4921 gagcttctga cgggcggtga ggattcgaca cctccctcgg agcccatcgg atcgaggaac
     4981 ggctccgggc tcggcgggat tctcgtgctc gagacgaggg atctcctctt cctcaataag
     5041 ccgcgcggcc tcctctcgca tgggccggga ggcctggacg agctggtcga agccgccatg
     5101 cgcgacagga tcggaatgtc actctcgttc cgaccgggtc ccctccacag gctcgacagg
     5161 aacacctcgg gcctgatcac cttcccccgg accgccgaag gatcgcgggc cttctccatg
     5221 atgctcaggt cgaggcggat ccggaagcgc tacctggccc tcctcgacgg ggttctggac
     5281 agggagctgc tgtgggagga tcggctcgtc cgggatgata ggacccggac gagccatgcg
     5341 ggggccgagg cggagcgctc ccccgacgag gcggcccggg gcagacgggc tgtctcgcgc
     5401 atcgtccccc tcatcggatc ccaagggctc tgcctcgcga tgatcgagat ccagaccggg
     5461 ctcacgcacc agatccgggc tcaggcgtcc ctccatggaa ggcccctcgc cggcgacagg
     5521 aagtacggtg gcagtcggca tgcctcgggc ttcctcctcc atgcccattc gatggagttc
     5581 tccgagcccc ccttcgacga cgtcccgcgt cgcgtcatcg ccagcctgcc gtccgaggcg
     5641 agcgaacggc tcgcgtcgat tttcgggcct aatgaggtgg aaaaggccca gagcctcgcc
     5701 ctcatgtctt gatttttggt ccggcccctg tactgtagaa ggctgggggt gccaatggga
     5761 gatcacatca atcgtccgat gaggaacgtg acaggattcc ggtcggctgt gaagccgacg
     5821 atggtcatca tcggaatcgc cgcggtcctc gtcctgattt tcctttctac gagcttcttc
     5881 atcgtggacc agacctcgag ggccgtcata acgaccttcg gcaagtacac gagggaagcc
     5941 gaaccggggc tccactacaa gatccccttc gggatccagc ggagctacaa tgtcccgaca
     6001 gaggtgatcc agaccgagca gttcggcttc aggaccgaaa aagcaggagt cgtgacccaa
     6061 tactccgaga cgtcctaccc gcaggaatcg gtcatgctca cgggcgatct gaacatcgtc
     6121 gatgtggaat ggatcatcca gtaccgcatc gtagacccga gggcctggct cttcaacacg
     6181 agcgaccgcc acaagacgat ccgcgacata tcgcagtccg tggtcaacct cctggttgga
     6241 gacaggacca tcctcggcgt cataggcccc gagcggacga cgatcgagga cagcgctgtc
     6301 cagatgatga acgagacctt caacaactat ggccttggca tacgggtgac ccaggtcaag
     6361 ctccagaaca tcgtcccgcc caaaggggtc caggatgcct tcgaggacgt gaacaaggcc
     6421 atccaggaca tgaaccgcct gatcaacgag ggcaaggagg cctacaactc cgagataccg
     6481 aaggcccagg gccaggccca gcaggccgtc gaggttgccc agggctacgc caccgagcgc
     6541 gtgaaccagg ctaagggcga cgtcgcccgc ttcacctcgg tctacgacga gtaccggaag
     6601 gcgccgggca tcacccggca gcgcctctat tacgagatga tggaggccgt gttcaagggc
     6661 gagaaggacg tcgagctcat cgacaagagg ttctcgaatt tcctgccgct caagaatctc
     6721 gagagcaagc cggcggcgac ccagggggca ggacaatgaa gcgcctcatc atcatcctcg
     6781 tcgtcatcgc catcgtggcc atcgccttct tcgcgaccgg tcccttctac gtcctgaacg
     6841 aaggcgagca ggccgtcgtc gtgcgcctcg gcaagatagt gggcgtcgag aaggacgccg
     6901 gcctcaagct gcgctccccc ttcctcgacg tcatcgtcaa atatccgaaa caggtaatgc
     6961 cctgggacgg agagccgcag aggatcccga ccaaggagaa ccagttcatc tgggtggaca
     7021 cgacggcccg gtggaagata accgatcccg tgaagttcta cgaatcggtg agcagcctcg
     7081 agaacgccta cggccggctc gacgacgtca tcgacaacgg cgtcaggacc gtgatctcgg
     7141 tcaactacct ccgcgaggcc gtgcgcaact ccaataacct cctgggcacc ggaacgaagg
     7201 agagcttcca gaccggagac gtggagggct cggctgccct ggagcagctg acccagagca
     7261 accagagcta cgagaccatc gacaagggca ggcggaggct ctccgaggag atgctcgcga
     7321 tcgtgaggcc catcgtcccg cagtacggga tcgaggtgat cgacatcgtg ccccggcaga
     7381 tcaagtactc ggacgagctc accgagagcg tctacaaccg gatgataaag gagcgcaacc
     7441 agatcgccca ggccttcagg agctacggcg agggcaagaa ggccgagtgg ctcgggaagc
     7501 ttgagaacga gaagcgctcg atcctttcgg gcgcctacca gaaggccgag accacgaagg
     7561 gaaaggccga cgctgaggcg acgcgcatct actcgagcgc gtacagccag gacccctcct
     7621 tcttcgattt ctggcgggca atcgaatcct accgccagac cctgccgagc ttctcgaaga
     7681 ccctttcgac cgacctggac tacttccgct acctctccag cccgcagggg cgctgaggcc
     7741 gtggcggggt cgggggcatc gcctcccggc cccgccggcc ttccatgccg catcgccggt
     7801 acccggccct cacgacacga gctcggcttt cgccgaccag gctccgctct ggcgatcgac
     7861 caggattcgg gcgaacctgc cttcgttcag aggcccgggg ttcaccacca gggtgccgcc
     7921 gacaacctgg gccgagggcg actcgtggat gtgtccgcat acccagagca ggggcctgaa
     7981 tgcgtccagg gccgcacgga gcgactcgga ccctaagggt gttccccttc gcaggtcggc
     8041 tccacagc
//
LOCUS       DASSQV010000143         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2274288, whole genome shotgun sequence.
ACCESSION   DASSQV010000143 DASSQV010000000
VERSION     DASSQV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274288"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFL04_02495"
     CDS             <1..381
                     /locus_tag="VFL04_02495"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7838602.1"
                     /translation="YGQLVEGAQAGLAPRLDVLLELHTGEESKTGFADTDSLLAACEA
                     ASAMNRISVRGLMTMAPLSPDQGIVRSSFRALKEAFALVQTRFGFPGFEVLSMGMSGD
                     FELAVEEGSTLIRVGTALFGSRTP"
     gene            419..1090
                     /locus_tag="VFL04_02500"
     CDS             419..1090
                     /locus_tag="VFL04_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838603.1"
                     /translation="MATTRIPSVASWPGHGALRIEDAMKDRGRRRGLAMAAALCLGLA
                     CSAWAQSSSPSQSPQTPGSGNPAPGSPVAAPGAMPPGFGPEQETPENPWRRFEIVSLG
                     SFPIMLFYTQFGFDFTLFVGHGFNKAYAPWPFKSVSSYSPNQGEQIARLGAALGLSFV
                     IGGIDAVIHAAKTRPRRPLPLEGTAPSPAPSPAPSPAPIEAPPPAPPGPGTPGPNAQS
                     PAGGG"
     gene            1138..1536
                     /locus_tag="VFL04_02505"
     CDS             1138..1536
                     /locus_tag="VFL04_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838604.1"
                     /translation="MATTRTKTLILFEDSKPDIERTAREIAERLDPERHELSLRAASE
                     VGISELLASALFFMGAEAPDAVAYREVARVFKGINLAGRRAAFFGASGSAVAWLRGLC
                     ADTEVSAAHADLVGRRPDHAALAAWLRGIA"
     gene            1604..2137
                     /locus_tag="VFL04_02510"
     CDS             1604..2137
                     /locus_tag="VFL04_02510"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET7838605.1"
                     /translation="MAERFDLDSAIRKVPDFPKKGILFYDITSILVKPEAFRHCIDEM
                     ARIYGGEKLDAVAAIESRGFLFAAPLCERLGIPLILVRKKGKLPGRTLSKRYDLEYGQ
                     AEIEMHQDDMPRGGRVLIVDDLIATGGTVRAAADLLREGGAVPIGVFAVIGLPFLDYE
                     KSLGGLPVRTLIDYHGE"
     gene            2164..>2525
                     /locus_tag="VFL04_02515"
     CDS             2164..>2525
                     /locus_tag="VFL04_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET7838606.1"
                     /translation="MTQALEKNPGWKGRRGPLVLVIMDGVGYGKYSEGDAVADSRMDA
                     LRAMEAASPRTRLRAHGTAVGLPSDEDMGNSEVGHNAIGCGRVYEQGAKLVGRSIETG
                     AMFRGGAWKRLVAQAKAGP"
BASE COUNT          368 a          926 c          850 g          381 t
ORIGIN      
        1 tacggccagc ttgtcgaggg agcccaggcg ggcctggctc cccggctcga cgtcctcctc
       61 gagctccaca cgggggagga atccaagacg ggcttcgccg acaccgattc cctcctcgcg
      121 gcctgcgagg cggcgagcgc catgaaccgg atctcggtca ggggcctcat gacgatggcc
      181 cccctgagtc ccgaccaggg gatcgtgcgc tcgtccttcc gagcccttaa ggaggccttc
      241 gccctcgtcc agacccgttt cggctttccc ggtttcgagg tcctctcgat gggcatgtcg
      301 ggcgatttcg agctggccgt cgaggagggc tcgaccctca tccgtgtggg cacggcgctt
      361 tttgggtcga ggacgccatg agggatcgag ggcggccggt tcggggcttg cgccgtgcgt
      421 ggccacgacg cggatcccga gcgtcgcgtc gtggccgggg cacggcgctc ttcggatcga
      481 ggacgccatg aaggatcgag ggcggcgccg gggcctcgcc atggccgccg cgctctgcct
      541 cggcctggcc tgctcggcct gggcccagag ctccagccca agccagagcc cccagacgcc
      601 gggctcgggg aaccccgccc cgggcagccc cgtcgccgcc cccggcgcga tgccccccgg
      661 tttcggccct gaacaggaga cgcccgagaa cccctggcgg cgcttcgaga tcgtgtcgct
      721 gggctccttc ccgatcatgc tcttctacac ccagttcggt ttcgatttca ccctctttgt
      781 gggccacggc ttcaacaagg cctacgctcc atggcccttc aagtcggtgt cctcctattc
      841 ccccaaccag ggcgagcaga tcgcgaggct cggcgcggcc cttggcctca gttttgtcat
      901 cgggggcatc gacgcggtga tccacgcggc gaagaccagg ccgcgcaggc ccctgcccct
      961 cgaggggact gccccgtcgc cggccccgtc cccggcccca tccccggccc cgatcgaagc
     1021 cccgccccca gccccgcccg gccccggcac gccgggcccc aacgctcaga gcccggctgg
     1081 cggcggctga ggcttcgaac ttgacagcgc aggggcgcgg gaatagtatt ggcccccgtg
     1141 gcgacgacca ggaccaagac cctcattctc ttcgaggact ccaagccgga catcgagagg
     1201 acggccaggg agatcgcgga gcgcctcgac ccggagcggc acgagctgag cctgagggcc
     1261 gcctccgagg tcggcatctc cgagctcctg gcctcggccc tgtttttcat gggtgctgag
     1321 gctcccgatg ccgtcgcgta tcgcgaggtc gcccgggtgt tcaagggcat caacctcgcg
     1381 ggacggcggg cggccttctt cggggcatcg ggctcggcgg tggcctggct ccgcgggctc
     1441 tgcgccgaca ccgaagtctc ggccgcccac gccgaccttg tcggccgccg tcccgaccac
     1501 gccgcgctcg cggcctggct ccgcggcatc gcctagcggg cgcccggacg gcccacttcg
     1561 ggacggtccg gggcgatccg actccttccg cagcgaggaa gccatggcag aacgattcga
     1621 ccttgacagc gcgatccgca aggtccccga tttcccgaag aaggggatcc tcttctacga
     1681 catcacgagc atcctcgtga aacccgaggc cttccgccac tgcatcgacg agatggccag
     1741 gatctacggg ggcgagaagc tcgacgcggt ggccgccatc gagtcccgcg gcttcctctt
     1801 cgcggccccc ctctgcgagc ggctcggcat ccccctcatc ctcgtgcgca agaagggcaa
     1861 gctccccggc aggaccttgt ccaagcgcta cgacctcgag tacggccagg ccgagatcga
     1921 gatgcaccag gacgacatgc cccgcggcgg cagggtcctc atcgtcgacg acctgatagc
     1981 gaccgggggc acggtgaggg cggcggccga cctcctccgc gagggcggag ccgtgccgat
     2041 cggcgtcttc gccgtcatcg gcctgccctt cctcgactac gagaagtccc tcggcggcct
     2101 gcccgtcagg accctcatcg actaccacgg cgagtgacgc cggcgcatga aggagcccca
     2161 gcaatgaccc aagccctcga gaagaatccc ggctggaaag gccggcgggg ccccctcgtc
     2221 ctcgtgatca tggacggcgt gggctatggc aagtactcgg aaggggacgc ggtcgccgat
     2281 tcgaggatgg acgccctgcg cgccatggag gccgcctccc ctcggacccg gctccgtgct
     2341 cacggcacgg ctgtcggcct gccatcggac gaggacatgg gcaactccga ggtgggccac
     2401 aacgctatcg gctgcggccg ggtctacgag cagggcgcca agctcgtcgg gcgctcgata
     2461 gagacggggg ccatgttccg gggcggggcc tggaagcggc tcgtggccca ggccaaggcc
     2521 gggcc
//
LOCUS       DASSQV010000144         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_35752, whole genome shotgun sequence.
ACCESSION   DASSQV010000144 DASSQV010000000
VERSION     DASSQV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35752"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFL04_02520"
     CDS             complement(<1..548)
                     /locus_tag="VFL04_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838607.1"
                     /translation="MKGKRRRRGTDAGRGALALGASVAIHAALFAALALSGRHPIHQG
                     PVQVVLSFADSEGGGQGDAVGLAPPSPDPSPAQVSGAGSANRGPAPQKPASATSPPER
                     DPGPRVADAESRGAPQEPRPLAAAEAGGPGSASDQVPPGSRQGGASSPRGTQGAGEGQ
                     SAPNTAAPDERILAERILAQRIL"
     gene            670..1161
                     /locus_tag="VFL04_02525"
     CDS             670..1161
                     /locus_tag="VFL04_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7838608.1"
                     /translation="MPSSVSLAELIERGGVYYNIGGATPMDALREGVRGLVLPKGIDA
                     EALLTAVLEREALMPTAIGNGVAIPHPRSPIVSKAELQRVAVLFLKTPIPYNALDRKP
                     VSVLFLILSADAKTHLAVLAAISHLCQRRDFQELLAERPLREELTAFIARLEASWEPV
                     REA"
     gene            complement(1158..1757)
                     /locus_tag="VFL04_02530"
     CDS             complement(1158..1757)
                     /locus_tag="VFL04_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HET7838609.1"
                     /translation="MSNSSYEKPDHWTIKAKKEGYPARSVYKLQEIDRKFGLVRKGMR
                     ILDIGAAPGSWSLWVLKRLEGTGLLMALDLQELGIEQGSPNFRFIRGDLYEPAVRSEL
                     LAQGPYNLVLSDAAPATTGNRVVDQGQSEAIVEAVIDYAEACLGPGGSMVAKIFQGGA
                     EAGLIKRLRGRFAAARGFKPEACRPGSFETYLVATGFKA"
     gene            1793..2845
                     /locus_tag="VFL04_02535"
     CDS             1793..2845
                     /locus_tag="VFL04_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET7838610.1"
                     /translation="MSSEYTEILGLIEREVLAVLPEEPDDSWFDRFFGALPASPRADI
                     RRALNAPALELVRRGGKRWRPLLLVLSARALGREDGALSISPVVEIAHNGTLIVDDIE
                     DGSDTRRGGPAIHLLHGIDASVNAGNLLYFLPLVLFDELRTSPATRLALHARYALHMR
                     RLHLGQAMDITWHRDHGSLPDRDSYLAMCALKTGVLARAAAELGALAALAPEAMVSGL
                     GSAFERVGVAFQILDDVANLRTGNPGKKRGDDIVEGKKSLPVILACEREAGLAPHLAL
                     LFDAARAGGPEAPAVEEAIGLIAGEGAVDAAEAIALEMLRESARMLSTLVPPGPASGP
                     LLGLVDALSGRGAAKL"
BASE COUNT          424 a         1074 c         1017 g          462 t
ORIGIN      
        1 aggatacgct gggcgaggat gcgctcggcg aggatgcgtt catcgggtgc ggcggtgttg
       61 ggtgcggact gcccctcgcc cgcgccctgg gttccgcgcg gcgatgaagc tccgccctgc
      121 cgcgagccgg gtgggacctg gtcggacgcg gagcccgggc cgccggcctc ggcggccgcg
      181 agggggcggg gctcttgagg agcgcccctg ctctcagcgt cggcgacccg aggcccggga
      241 tcccgctcgg gcgggctcgt ggctgaagcg ggcttctgcg gagccgggcc ccgattcgct
      301 gatccggcgc cgctgacctg agccgggctc gggtctgggg agggcggcgc gaggcccacc
      361 gcatcgccct gtccgccgcc ctcagaatcc gcgaagctga ggacgacctg gacggggccc
      421 tgatggatgg gatgcctccc ggacagggcc agggcagcga agagggcggc gtggatcgcg
      481 acggaggccc cgagcgccag cgcgccgcgg ccggcatcgg tcccccggcg tctgcgtttc
      541 cctttcatgg atggctcggg cggaagtata gcaggggctt tcccgcagtg tatacatgag
      601 ccaggacggg cgatcgagcc ttgccccgcc ccggcccaag ggccatactt ggggcggagg
      661 ccgaagctca tgccatcatc agtatcgctt gcggagttga tcgagagggg cggggtctac
      721 tacaacatcg ggggagcgac gccgatggac gccctccgcg agggcgtcag gggcctcgtg
      781 ctgcccaagg ggatcgacgc cgaggccctg ctgaccgccg tcctcgagcg cgaggccctc
      841 atgcctaccg ccatcggcaa cggggtggcc atcccccatc ctcggagccc catcgtgtcc
      901 aaggccgagc tccagcgggt ggccgtcctc ttcctgaaga cgcccatacc ctacaacgcc
      961 ctcgaccgca agcccgtgtc ggtgctcttc ctcatcctct cggccgacgc gaagacccat
     1021 ctcgccgtgc tcgcggcgat atcccatctc tgccagaggc gggatttcca ggagcttctc
     1081 gccgagcgcc ccctgaggga ggagctgacc gccttcatcg cccgtctcga ggcctcctgg
     1141 gagcccgtga gggaggccta ggccttgaac cccgtagcga ccagataggt ctcgaaggag
     1201 cccggccggc aggcctcggg cttgaagccc ctggccgcgg cgaagcggcc gcggagccgt
     1261 ttgatcaggc cggcctcggc cccgccctgg aagatcttgg ccaccatgga gccgcccggg
     1321 cccagacagg cctcggcgta gtcgatgacg gcctccacga tcgcctcgga ctggccctgg
     1381 tcgaccacgc ggttgcccgt cgtcgcgggg gcggcgtcgg agaggacgag gttgtagggc
     1441 ccctgggcga gaagctcgct cctcaccgcc ggctcataga ggtcgccgcg gatgaagcgg
     1501 aagttgggcg agccctgctc gatgccgagc tcctggaggt cgagggccat gaggaggccc
     1561 gtgccctcga ggcgcttcag gacccagagg ctccatgagc ccggggccgc gccgatgtcc
     1621 aggatcctca tgcccttcct gaccaggccg aacttcctgt cgatctcctg gagcttgtag
     1681 accgagcggg cgggatagcc ctccttcttc gccttgatcg tccagtgatc gggcttctcg
     1741 tagcttgagt tcgacatgga ctaatccccc cggcccgcgc tatggtgggc ccatgagcag
     1801 cgagtatacg gaaatcctcg gcctcattga aagggaggtc ctggccgtcc tccccgagga
     1861 gcccgacgac tcctggttcg accgcttctt cggggccctg cccgcctcgc cccgcgccga
     1921 catcagaagg gccctcaacg cgcccgccct cgagcttgtg cggaggggag gcaagcgctg
     1981 gcgccccctc ctccttgtcc tgtccgctcg agccctcggc cgcgaggacg gggccctctc
     2041 gatcagcccc gtcgtcgaga tagcccacaa cgggaccctc atcgtggacg acatcgagga
     2101 cggctcggac acgaggaggg gagggcccgc catccacctc ctccacggga tcgacgcctc
     2161 ggtgaacgcg gggaacctcc tgtatttcct gcccctcgtc cttttcgacg agctgcgcac
     2221 ctcgcccgcg accaggctcg ccctccatgc gcgctacgcc ctgcacatgc gccgcctcca
     2281 cctgggccag gcgatggaca tcacctggca ccgcgaccac ggcagcctgc ccgaccgcga
     2341 cagctatctc gcgatgtgcg cactcaagac tggggtgctc gcgagggcgg ccgccgagct
     2401 cggtgccctc gccgccctgg ctcccgaggc catggtctcc ggtcttggtt cggccttcga
     2461 gagggtcggc gtggccttcc agatcctcga cgacgtggcc aatctcagga cgggcaaccc
     2521 cggcaagaag cgcggcgacg acatcgtcga gggcaagaag agcctgccgg tcatcctcgc
     2581 ctgcgagcgc gaagccgggc tcgcccccca tctggccctc ctcttcgacg ccgcccgcgc
     2641 cgggggcccc gaggcgccgg ccgtcgagga ggcgataggg ctgatcgctg gggagggcgc
     2701 cgtcgacgcc gccgaggcca tcgccctcga gatgctgcgc gagtccgcgc gcatgctctc
     2761 gaccctcgtc ccccccggcc cggcctccgg gcccctcctc ggccttgtcg acgccctctc
     2821 cggaaggggg gcagcaaagc tttgatttcc cccctcccgc cgctatacta ggtacgggag
     2881 ggccgatggc gtcgcagacc ttcgtcgagg ccgatatctg gaccgtcgct cagaccgacc
     2941 agggcaatgt tgtcctggtg aggcctaagg gctctga
//
LOCUS       DASSQV010000145         5647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1030627, whole genome shotgun sequence.
ACCESSION   DASSQV010000145 DASSQV010000000
VERSION     DASSQV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030627"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VFL04_02540"
     CDS             <1..1224
                     /locus_tag="VFL04_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838611.1"
                     /translation="GLAMGVTIQAKPEFNRDYHGGYFAPSNAFPIMAEEQDFLTRGVL
                     SWTSPHLDLAIGRDRVGYGDDVRGSIMPGSSLPYLDAIKGELRLGPARLDWMVANIES
                     KQSWEARAFGSDYDVEPNLGVAPPTSLFGFETSDTPTAIYEMFHRISWDFGALRFELA
                     ENYIVARPSNRLVVTDLLPLSVWHNANEVPNNDTLYINATWKPSEDLKLAFQGGFDDI
                     NGEILGIPDSGNTIPAAVLGLTWAKDTGAGEFGLYTELGYTHYLWGSFSAHGQIGIKY
                     DDPLARAIYRLDLDNGAGLIPLTSPYGPGALWFLASGGLELGGSGLRLGTELLVLGKN
                     TEANLIATPYDKTIQDAPWELFASLKLPVRYRTGSIDLTASPDLCLRNRQLWLEAAFV
                     LGYHFRAARVIDDRP"
     gene            1384..2769
                     /locus_tag="VFL04_02545"
     CDS             1384..2769
                     /locus_tag="VFL04_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7838612.1"
                     /translation="MQSVLPIAVAVVVLLAFALLLIVLSSSRKTQNGGRGKKLKTRDK
                     AQILKEANRRLASNPKDLDALTALGDIAWEEKDWERALKTYESLAEAGAGNTEVDEFM
                     ANTRYGIAALRLNRLDEAYKGLIVARTIKQDDFEVNFNLGYLEFQRKAYEKSASLLRQ
                     AVQVNPEHAASLRYLGHSNFKLKLYKDALTMLRRAIDLEPEDKESLFAAGECYYELGN
                     LEQALKIFTHLRADPQLGPSAALFAGTVHLNQKLYDRAIADFEIGLRHQNTRIETVVE
                     LKYRLSAAYLKTQDIGKAVALLNDVQSLYPNYKDVPALLGKYRELNSNRNLQTFLLGS
                     TSDFVTLCRKISLMFFPKAKVKITDISVQGNDWADVLAEVETTKWSDLILFRFIRSQG
                     NVGELAVRDFHARVKDLKAGKGYCITAGAFSEEAKRFVEARLIDLIAKDTLMGLLNTI
                     DSRAKGLLAES"
     gene            complement(2811..3500)
                     /gene="rsmG"
                     /locus_tag="VFL04_02550"
     CDS             complement(2811..3500)
                     /gene="rsmG"
                     /locus_tag="VFL04_02550"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET7838613.1"
                     /translation="MTTPPKLLCRGLSLLGLPPEGRLADALALYLAEIEAWNPAYGLV
                     GATGDELIVKHVLDSLAPLSILRAELARTAEGRSLSPGFGLAPPRMRIADLGTGAGLP
                     GIPLALALPETEVSLIDRMGKRIRFLESQKALLRLDNAEVIESEVERARGSYDLVTFR
                     AFRPFERKLFKSVFALCESDGVVFAYKGRKDKAEAELSEIEGLYSSVRILPVQVPFLE
                     DERCIVALSPA"
     gene            3650..4606
                     /locus_tag="VFL04_02555"
     CDS             3650..4606
                     /locus_tag="VFL04_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7838614.1"
                     /translation="MRIAVVLPAYNEELCIEQVILDFHRHLPEALIHVVDNNSSDGTG
                     SIARAVMASHSIAGSVMFVREQGKANAVRRAFEEIDADLYVMADADTTYAGSEARTLI
                     QPVAEGRADMVVGDRHAAGVYREQNKRRFHDFGNNLVKNVINLLFRSELHDVLSGYRV
                     FSRRFVKNFPILSKGFDLETEMSVHALSHRFSVLELPISYKDRPAGSFSKLNTFSDGI
                     LVMRKILGLFIAHSPLRFFNVFCALSALVSLAVGISPVLEYLETGFVKKLPSLVAASG
                     FLVLALLFFAIGLLLEMSRQYQLTNYHHRVLNFTALDRRRGD"
     gene            4596..4994
                     /locus_tag="VFL04_02560"
     CDS             4596..4994
                     /locus_tag="VFL04_02560"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET7838615.1"
                     /translation="METDARRSLPSKLGRFLVAGGLAFLVDFAVYWLLGLLIGRFWAK
                     LSSFVAATVFSFVVNKTLTFGAKRVGASRIALFAAYYAMMTFVNPAINELAFGSLGAK
                     LPAFVLTTAVCAVMNFSFMNLVIFREARRI"
     gene            5003..>5647
                     /locus_tag="VFL04_02565"
     CDS             5003..>5647
                     /locus_tag="VFL04_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838616.1"
                     /translation="MIYLYVALFLFAPVCAHAYLDPGTGSLLLYALAGIATSLVLFLR
                     GLWYSIRSRVLFRGAPAVSRSLPDIVFHSEGGKYWQVFQPVIAELDRRGIDCAYVTPD
                     AGDPALTSRHGRLTAFRPGNEVVTIAHMNALKAPLVVSTTPHLDVYMLRRSKGVKHYA
                     HLFHAPTDIPFYEKYAFDWYDSMLTVGPFQEPKLRALEAARSRPSKRLYETGCTY"
BASE COUNT         1019 a         1941 c         1710 g          977 t
ORIGIN      
        1 ggcctggcca tgggcgtgac gatccaggcg aagcccgagt tcaaccggga ctaccacggc
       61 ggctacttcg ccccaagcaa cgccttccca atcatggccg aggagcagga cttcctgacc
      121 cgcggcgtcc tgtcatggac cagcccccat ctcgacctcg ccatcggccg cgacagggtc
      181 gggtacggcg acgacgtcag gggcagcatc atgccgggct cgagccttcc ctatctggac
      241 gcgatcaagg gcgagctgag gcttgggccg gcgaggctgg actggatggt ggcgaacatc
      301 gagtcgaagc agtcctggga ggccagggcc ttcggctccg actatgacgt cgaacccaat
      361 ctaggcgtgg ccccgcccac ctcgctcttc ggcttcgaga cgagcgacac acccacggcc
      421 atctacgaga tgttccaccg catctcctgg gacttcggcg ccctccgatt cgagctggcc
      481 gagaactaca tagtcgcgcg gccctccaac aggctcgtgg tcaccgacct cctccccctc
      541 tcggtctggc acaacgcgaa cgaggtgccc aacaacgata ccctctacat caacgctacc
      601 tggaagccga gcgaggacct gaagctggcc ttccagggcg gcttcgacga catcaacggg
      661 gagatcctcg gaatcccgga ctcgggcaac acgatacccg cagccgtgct gggactcact
      721 tgggccaagg atacgggggc cggcgagttc ggcctgtata cggagctcgg ctacacccac
      781 tatctctggg gcagcttcag cgcccatggg cagatcggca tcaagtacga cgaccccctg
      841 gcccgcgcca tctaccgcct cgacctcgac aacggggccg gcctcatccc gctcacgagc
      901 ccctatggac cgggcgccct ctggttcctc gcctcgggcg gcctcgagct cggcggctct
      961 ggcctcaggc tcggcacgga actcctggtc ctcggcaaga acaccgaggc caacctcatc
     1021 gccacgccct acgacaagac catccaggac gcgccctggg agctcttcgc ctcgctcaag
     1081 ctccccgtgc gctacaggac cgggtccatc gatctcacgg cgagccccga cctctgcctc
     1141 aggaaccggc agctctggct cgaggcggcc tttgtcctcg gctaccattt ccgcgccgcg
     1201 agggtgatcg acgaccgccc ctaggccggg tcagacggca cacgaacccc ggggagaccc
     1261 ggggtttttt tgtccctggg gaccaagaac ccttccctgg ccggggcccg ggaaggggtc
     1321 cggcgcaggc tggcccgcct tcggaggttg acaccgcagg ccacaggccc cactatttac
     1381 cgcatgcaga gtgtcttgcc catcgcggta gcggtcgtcg tcctgctcgc cttcgcgctt
     1441 ctcctcatcg tgctcagctc atcgcgcaag acccagaacg ggggccgcgg caagaagctc
     1501 aagaccaggg acaaggccca gatactcaag gaggccaacc gcaggctggc ctcgaaccca
     1561 aaggacctcg acgcgctcac cgccctcggc gacatagcct gggaggagaa ggactgggag
     1621 cgggcgctca agacttacga aagcctcgcc gaggccggag ccggcaacac cgaggtcgac
     1681 gagttcatgg ccaacacccg ctacggtatc gcggccctgc gcctcaaccg gctcgatgag
     1741 gcctacaagg gcctgatcgt ggccaggacg atcaagcagg acgacttcga ggtgaacttc
     1801 aacctcggct atctcgagtt ccagaggaag gcctacgaga agtcggcctc cctcctcagg
     1861 caggccgtgc aggtaaaccc cgagcacgcc gccagcctgc gctacctcgg ccactcgaac
     1921 ttcaagctca agctgtacaa ggacgccctc acgatgctca ggagggcgat cgacctcgag
     1981 cccgaggaca aggagtcgct cttcgccgcg ggggagtgct actacgagct cggcaacctc
     2041 gagcaggccc tcaagatctt cacgcatctg agggccgacc cccagctcgg cccctcggcc
     2101 gcactcttcg cggggaccgt gcacttgaat cagaagttat acgaccgcgc catcgccgat
     2161 ttcgagatcg ggctcaggca ccagaacacg aggatcgaga ccgtggtcga gctcaagtac
     2221 aggctctcgg ccgcctacct caagacccag gacatcggca aggccgtggc tctgctgaac
     2281 gacgtgcaga gcctctaccc caactacaag gacgtcccgg ccctcctcgg caaataccgc
     2341 gagctcaact ccaaccgcaa cctccagacc tttctcctgg gctcgacgag cgacttcgtc
     2401 accctctgcc ggaagatcag cctcatgttc ttcccgaagg cgaaggtgaa gatcaccgac
     2461 atctcggtcc agggcaacga ctgggccgac gtcctggccg aggtcgagac cacgaaatgg
     2521 tcggacctga tcctcttccg cttcatccgc tcccagggca acgtcggcga gctcgcggtc
     2581 cgcgacttcc acgccagggt caaggatctc aaggcgggca agggctactg catcaccgcg
     2641 ggggccttca gcgaggaggc gaagcgcttc gtcgaggcga ggctcatcga ccttatcgcg
     2701 aaggacaccc tcatgggcct cttgaacacg atagactcga gggcaaaggg cctgctcgcg
     2761 gagagctgag ggcggtcggc cggttggggg aggccggcgg accaacaggg ctaggccggg
     2821 ctgagcgcca cgatgcagcg ctcgtcctcg aggaagggca cctggacggg cagtatccgg
     2881 accgatgaat agaggccctc gatctccgac aactcggcct cggccttatc cttcctgccc
     2941 ttgtaggcga agaccacacc gtcgctttcg cacaacgcga agacactctt gaagagcttc
     3001 ctctcgaagg ggcggaaagc cctgaaggtc acgaggtcat agctgcccct ggcccgctcg
     3061 acctcggact cgatgacctc ggcgttgtcg aggcggagga gggccttctg gctctcgagg
     3121 aagcggatgc gcttgcccat gcggtcgatg agcgagacct cggtctcggg cagggcgagg
     3181 gcgaggggga tgccggggag gcccgctccg gtgccgaggt ccgcgatcct catccggggc
     3241 ggcgccaggc cgaagcccgg ggaaagcgat cggccctccg ccgtccgcgc cagctcggcg
     3301 cgaaggatcg acaggggggc gaggctgtcg aggacgtgct tgacgatgag ctcgtcgccc
     3361 gtcgcaccca cgaggccata agctgggttc caggcctcga tctcggcgag gtagagcgca
     3421 agggcatcgg cgagccggcc ttcggggggg aggccgagga gggacaagcc gcggcaaagc
     3481 agcttgggtg gcgttgtcat cgggacataa gtatagcccg aacaagccct gggggagaag
     3541 tccgcgcccc cttgagccgt ccttagtcct aagggtctcg acttgacatc ctcactccat
     3601 tcggacatct tcaatccctg atgaagaacg gggaaaagcg gagcaccaca tgagaattgc
     3661 ggtcgtactt ccggcctaca acgaggagct ctgcatcgag caggtcatcc tcgatttcca
     3721 tcggcacctg cccgaggcgc tcattcacgt ggtcgacaac aactcgagcg acggcacggg
     3781 ctccatcgcc cgcgccgtga tggcatccca ctcgatcgcg ggctcggtga tgtttgtgcg
     3841 ggagcagggc aaggccaacg ccgtccgccg cgcatttgag gagatcgacg ccgacctcta
     3901 tgtaatggcc gacgccgaca ctacctacgc cgggagcgag gcccggaccc tgattcagcc
     3961 cgtcgccgag ggccgggccg acatggtggt cggcgaccgc cacgccgcgg gggtataccg
     4021 cgagcagaac aagaggcgct tccatgactt cggcaacaat ctcgtcaaga acgtgatcaa
     4081 cctcctgttc cggtccgagc tccacgacgt cctgagcggc taccgggtgt tctcacggcg
     4141 ctttgtcaag aatttcccga tcttgagcaa gggcttcgac ctcgagaccg agatgtcggt
     4201 gcatgccctg agccatcgct tctccgtgct cgagctgccg atctcttaca aggaccggcc
     4261 cgccgggtcc ttctccaagc tgaacacctt cagcgatggc atcttggtca tgcggaagat
     4321 cctgggcctc tttatagccc acagccccct tcgcttcttc aatgtcttct gcgccctctc
     4381 ggccctggtc tcgctcgcgg tgggcatctc ccccgtcctc gagtacctgg agaccggctt
     4441 cgtgaagaag cttccgtcct tggtggcggc ctcgggcttc ctggtgctgg ctctgctctt
     4501 cttcgcgatc ggcctcctgc tcgagatgag ccggcagtac cagctgacca actaccacca
     4561 ccgggtcctg aacttcacgg ctctggacag acgccgtgga gactgatgcg cggcgctccc
     4621 tgccatcgaa gctgggccgc ttcctggtcg cgggaggcct cgcctttctc gtggacttcg
     4681 cggtctactg gctcctcggt ctgttgatcg gccgcttctg ggccaagctc agctccttcg
     4741 tcgcagccac cgtcttctca tttgtggtca acaagaccct gaccttcggg gcgaagcggg
     4801 tgggggcctc caggatcgcc ctgttcgccg cctactacgc gatgatgacc ttcgtcaatc
     4861 ccgccatcaa cgagctcgcg ttcggctcgc tgggtgccaa gttgccggcc ttcgtgctca
     4921 cgaccgccgt ctgcgccgtc atgaacttca gcttcatgaa tctcgtcatc ttccgcgaag
     4981 cgcgccggat ttaggagggc gggtgatata cctctatgtt gcgctattcc tctttgcgcc
     5041 ggtctgcgcc cacgcctacc tggatcccgg tacgggcagc cttcttctgt acgccctcgc
     5101 gggcatcgcc acctctcttg tcctcttcct gagagggctc tggtactcca tcaggagcag
     5161 ggtcctcttc cggggggctc cggccgtctc gaggagcctg cctgacatcg tcttccattc
     5221 ggaggggggg aagtactggc aggtgttcca gccagtcatc gctgagctcg accgacgcgg
     5281 catcgactgt gcctatgtga cccccgacgc cggggatcct gcactgacga gcagacacgg
     5341 gaggctgacc gccttccggc ccggcaacga ggtcgtgacc attgcgcaca tgaacgcgct
     5401 caaggccccc ctcgtcgtgt ccacgacccc ccacctcgat gtctatatgc tgcggcgctc
     5461 caagggggtg aagcactacg cccatctttt ccacgcgccg accgacatcc ccttctacga
     5521 gaagtacgcc ttcgactggt acgattctat gctcaccgtc ggccccttcc aggagcccaa
     5581 actccgggcc ctcgaggccg cacgatcgag gccgtccaag cgcctgtacg agacgggctg
     5641 cacctac
//
LOCUS       DASSQV010000146         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2275233, whole genome shotgun sequence.
ACCESSION   DASSQV010000146 DASSQV010000000
VERSION     DASSQV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2275233"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VFL04_02570"
     CDS             <1..1062
                     /locus_tag="VFL04_02570"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838617.1"
                     /translation="FRHCVSGASREALDSLDAILARGVSVEQLVVDGVDYFRSLLLLK
                     HGISRPSLIGAPAGAYEQSVLDALSPEQIERGLASLLELYRNLRSSVEPRFELELTVA
                     RLCRLAQYVSQGEVAEVIASLKRRVEGEAGPGAGPAKVGPALPDAAAVEKKKHELPPS
                     LTAAFGSLAAAASVHDGQDGGPVPEAKPLPRPPARPLAMNDDAAVEPERPAEEAAAPE
                     PGAAEAPADDPLRASVIEALGRSSPLLKTGLSASLPWRVEGPKLVIPFRSGIDESVVQ
                     GSIAAIASKAGELLGRTVKVELIVEPRSPRPATRPGPIGGQEDQGAEPPADSASVVQR
                     VFRGTLVETRKQGERDEPR"
     gene            1049..1381
                     /locus_tag="VFL04_02575"
     CDS             1049..1381
                     /locus_tag="VFL04_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET7838618.1"
                     /translation="MNLAEMMEMLRNPQAIQARVNELKEKTARITATGSSGGGMVRIT
                     MNGGLEMLSCEIASEVVDPSDIGLLQDLVRAAYNDACARVKEEIQKELSSGLEGLPIP
                     PGMLGGLG"
     gene            1378..1965
                     /gene="recR"
                     /locus_tag="VFL04_02580"
     CDS             1378..1965
                     /gene="recR"
                     /locus_tag="VFL04_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET7838619.1"
                     /translation="MKALEELVGLLTRLPGIGRKSALRVAYHLLKTDAAFAEALADRI
                     TGIKRAIVFCSICGSYAEEDPCPVCSSTTRERSIICAVEQPQDVVSIEASREYRGLYH
                     VLGGLISPLDGVGPEDLRIQSLIARIREGGVSEVVVATNPTVEGDTTALYLKRLLEPT
                     GVSVTRLATGIPVGGDLEYADRLTLARSFRGRIRL"
     gene            complement(2055..>2905)
                     /locus_tag="VFL04_02585"
     CDS             complement(2055..>2905)
                     /locus_tag="VFL04_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838620.1"
                     /translation="GRSRGDTDDEILSALSGLLATSYSYSVSGDIYMQVMWRRLKARM
                     EAGVIAGRLAAPGPESRVRRPSGPRPPAPEAPAPEPAPVQRSPEAAAERSPTPAKQTP
                     RPVRAAKAPGGTPPAPRAASGPPPAGRTPANRLPGTRVRQAEANPSPPSLERAGQLHG
                     ERLLKPSPVGAEPLPEIVGRQGSISDRIRALSGKAYDVYKVIFLERVREDIRQALLAN
                     QTRSHRMFDTAANEAEDQVYAFMTAHYDDPFMDWARSAEREAVELRGFSLPSLDPVIK
                     ACYEKL"
BASE COUNT          433 a         1002 c         1017 g          453 t
ORIGIN      
        1 ttccggcact gcgtctccgg ggcctcgcgt gaggccctcg actcgctcga cgcgatcctg
       61 gcccgggggg tctccgtcga gcagctcgtg gtcgacggcg tcgactactt ccgctcgctc
      121 ctcctgctca agcacggcat ctcccggccc tccctcatcg gcgccccggc cggggcctat
      181 gagcagagcg tcctcgatgc cctttcgccc gagcagatcg agcgcggcct cgcctccctc
      241 ctcgagctct accgcaacct ccgctcgagc gtcgagccgc gcttcgagct cgagctcacg
      301 gtcgcgaggc tctgcaggct tgcccaatac gtctcccagg gcgaggtcgc cgaggtcata
      361 gcctcgctca agcgccgggt ggagggcgag gcgggcccgg gagcaggtcc ggccaaggtt
      421 ggtcccgcgc tccccgacgc ggcagccgtc gaaaaaaaaa agcatgagct tccgccttca
      481 ctgacggcgg ccttcggcag cctcgcggcg gcagcctcgg tccatgacgg gcaggacggc
      541 ggcccggtgc ccgaggccaa gcccctcccg cggcctcccg ccaggcccct cgccatgaac
      601 gacgacgcgg ccgtggagcc ggagcgaccc gcggaagagg ctgccgcccc tgagccgggc
      661 gctgccgagg ccccggctga cgatcccctg cgcgcgagcg tcatagaagc cctggggcga
      721 tcgagtcccc tgctcaagac cgggctttcg gcctccctgc catggcgggt cgagggaccc
      781 aagctggtca taccattccg ctcgggcata gatgagtccg tggtccaggg aagcatcgcg
      841 gccatcgcgt cgaaggccgg ggagctcctg ggccggacgg tcaaggtcga gctcatcgtt
      901 gagccccgca gcccaagacc ggcgacgcga ccgggtccca taggcggcca ggaggaccaa
      961 ggggccgagc cgcccgcgga ttcggcaagc gtcgtacaga gggtattccg cgggacgctg
     1021 gtggaaacac gaaagcaagg agagcgcgat gaacctcgct gagatgatgg aaatgctccg
     1081 caatccgcag gccatacagg ctcgggtcaa tgaactcaag gagaagaccg ccaggatcac
     1141 ggccacgggc agctcgggcg gggggatggt caggatcacg atgaacggcg gcctcgagat
     1201 gctgtcctgc gagatcgcga gcgaggtggt ggacccgagc gacatcggcc tccttcagga
     1261 ccttgtgcgc gcggcctaca acgacgcctg cgcccgcgtt aaggaagaga tccagaagga
     1321 gctgagctcc ggccttgagg gcctgccgat cccgcctggc atgctcgggg gccttggttg
     1381 aaggcactcg aggagctcgt cggcctcctc acccgcctcc cagggatcgg gaggaagagc
     1441 gccctccgcg tggcctatca ccttctcaag accgacgccg ccttcgccga ggccctcgcc
     1501 gaccggatca cggggataaa gcgcgccatc gtcttctgct cgatctgcgg ctcctatgcc
     1561 gaggaggacc cctgtccggt ctgctcctcg acgacccgtg agcgctcgat catctgcgcc
     1621 gtcgagcagc cccaggacgt cgtcagcatc gaggcctcgc gcgagtaccg aggcctctac
     1681 cacgtcctcg gtgggctcat ctcgcccctc gacggggtgg ggccggagga tctcaggatc
     1741 cagtcactca tcgcccggat ccgagagggc ggggtcagcg aggtcgtggt cgcgaccaac
     1801 ccgacggtcg agggcgacac gacggccctg tacctgaagc ggctcctcga acccaccggg
     1861 gtgtcggtga ctcggctcgc gacggggata cccgtcggtg gagacctcga gtacgcggac
     1921 cggctgacac tggcccggtc cttccgggga cgcatcaggc tctaggcctg gccttcccaa
     1981 gcccggcgcc cttggccgcg gcgcccgccg ggcgcaggcc accccagccg cgacgccgcg
     2041 cgatgggcgc gcattcagag cttctcgtag caggccttga tgacgggatc gaggctcggc
     2101 agggagaatc cacgcagctc caccgcctcc ctctcggccg acctggccca gtccatgaag
     2161 ggatcatcgt agtgggcggt catgaaggcg tagacctggt cctcggcctc gttggcggcc
     2221 gtgtcgaaca tgcggtggct cctggtctgg ttggcgagga gggcctgcct gatgtcctcc
     2281 ctgacccgct cgaggaagat caccttgtag acatcgtagg ccttccccga cagggctctg
     2341 atcctgtccg agatggagcc ctgtctgccg actatctcgg gcaggggttc ggcgccgacg
     2401 gggctcggct tgaggaggcg ctccccgtgg agctggccgg cccgctccag gctcggtggg
     2461 gacgggtttg cctcggcctg tctgaccctt gtgccgggaa gcctgttggc gggagtcctg
     2521 ccggccgggg gcgggcctga tgctgccctt ggagcgggtg gagtgcctcc gggggctttg
     2581 gccgcgcgga ctgggcgggg cgtctgcttt gctggggtgg gagaacgctc ggccgccgcc
     2641 tcggggcttc gctggaccgg ggcgggctcg ggggccggag cctccggggc ggggggcctg
     2701 gggccagatg gcctcctcac gcgcgattcg ggcccaggcg cagcgagcct gcccgcgatg
     2761 acgcccgcct ccatgcgtgc cttgagcctt ctccacatca cctgcatgta gatgtcgccg
     2821 cttacggagt aggaatagct ggtggccaga aggccagaga gggcgctcag tatctcgtcg
     2881 tcggtgtctc cccgcgagcg gccga
//
LOCUS       DASSQV010000147         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1901303, whole genome shotgun sequence.
ACCESSION   DASSQV010000147 DASSQV010000000
VERSION     DASSQV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901303"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VFL04_02590"
     CDS             complement(<1..954)
                     /locus_tag="VFL04_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7838621.1"
                     /translation="MKRFWAVFVARNLEFFRDKSTLIWNLALPVMLIFGFAFAFPSGG
                     GTTFKVGVVGQTPAAGSGADVAFLHYKYLQFVPYDSLATPLDRLAHHQLDMVLDFQKA
                     AYYVNETSSNGYVLERMLNPGGGAGFYAKQTVTGSAIRYVDWFLPGLIGINILFGSLS
                     GVGFVIVRYRRNGVLKRFKASPLSAFEFVSSQVVSRFFIIIVMSAAVFLGTNLFLHFR
                     MVGSWGLLLLVNALAILCMIALGLVFSSRIRSEEVAGGLMNLITWPMMIFSGVFFSLE
                     GTPELMRAASRAFPITYYIEASRSFMLDGAGLAAIGGDLAVL"
     gene            complement(977..1873)
                     /locus_tag="VFL04_02595"
     CDS             complement(977..1873)
                     /locus_tag="VFL04_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7838622.1"
                     /translation="MSQASDTSAILEVRDLRKAYGKVKAVDGISFSVREGSCFGILGP
                     NGAGKTTAIEVIETIHAPDSGQILYRGAPVSRSFKENIGIQFQDTSLQEFLTVRETLE
                     LFAKLYPNPRPMADIIEACSLEEFIDRDNRKLSGGQRQRMLLGIALVNDPDLLFLDEP
                     TTGLDPHARRNFWRLIEGIKARGKTVILTTHYMDEAEYLCDEIIVMDHGRIIETGRPR
                     ELLLKHFGEAVIRIPAAAVPKAAAFSCPAEAEEGFVALRCDDTDASLRELVTMDIDLS
                     GITVERRNLDDLFLKLTGSGLE"
     gene            complement(1940..>2303)
                     /locus_tag="VFL04_02600"
     CDS             complement(1940..>2303)
                     /locus_tag="VFL04_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838623.1"
                     /translation="AFQGMRTEIAEVVSSFEEIERSVGELKVGGTQILESVSTLREIS
                     SRVSGSSLTISAETESVGQAMAGVKELFAETRTVTADMTEKVRTVGSCTEDSGRKSRE
                     IDAVTTRISESLAAFRTE"
BASE COUNT          394 a          710 c          822 g          377 t
ORIGIN      
        1 gaggacggcg aggtcgcccc cgatggcggc caggcccgcg ccgtcgagca tgaacgagcg
       61 cgacgcctcg atgtagtagg tgatcgggaa ggcccgagag gccgcgcgca tgagctccgg
      121 cgtcccctcg agcgagaaga agacccccga gaagatcatc atgggccagg tgatcaggtt
      181 catgaggccg cccgcgacct cctcgctcct gatccgcgag gagaagacga ggccgagggc
      241 gatcatgcag aggatcgcga gggcgttcac gaggaggagg aggccccacg agcccaccat
      301 gcggaagtgg aggaagaggt tcgtgccgag gaagacggcg gccgacatca cgatgatgat
      361 gaagaagcgc gagaccacct gggagctcac gaactcgaag gcggagaggg ggctggcctt
      421 gaagcgcttc aggaccccgt tgcggcggta gcgcacgatg acgaagccca cgcccgagag
      481 gctcccgaag aggatgttga tcccgatgag gccgggcagg aaccagtcga cgtagcggat
      541 ggccgagccc gtgactgtct gcttggcgta gaagcccgcc cccccgccgg ggttcagcat
      601 ccgctcgagg acgtagccgt tggaggaagt ctcgttgacg tagtaggcag ccttctggaa
      661 gtcgaggacc atgtcgagct ggtggtgggc gagtctgtcc aggggcgtgg ccagggaatc
      721 gtagggcacg aactgcaggt acttgtagtg gagaaaggcc acgtcggccc cgctacccgc
      781 cgcgggggtc tggcccacga cgcccacctt gaaggtggtg cctcccccgc tcgggaaggc
      841 gaaggcgaag ccgaagatca gcatcaccgg gagggcgagg ttccagatga gggtcgactt
      901 gtcgcggaag aactcgaggt tcctcgcgac gaacacagcc cagaaacgct tcatggcttg
      961 ctccgcgcgg cgcgcatcat tccaggcccg agcccgtcag cttgaggaag aggtcgtcga
     1021 ggttgcgccg ctcgacggtg atcccgctca agtcgatgtc catcgtcacc agctcgcgca
     1081 gggacgcgtc ggtgtcgtcg cagcgcaggg cgacgaagcc ctcctcggcc tcggccgggc
     1141 aggagaaggc ggccgccttg ggcacggcgg ctgcggggat gcggatcacc gcctcgccga
     1201 agtgcttcag gaggagctcg cgcggcctgc ccgtctcgat gatgcggccg tggtccatca
     1261 cgatgatctc gtcgcagagg tactcggcct cgtccatgta gtgggtcgtg aggatgacgg
     1321 tcttgcccct cgcctttatg ccctcgatga ggcgccagaa gttgcgcctc gcgtgggggt
     1381 cgagccccgt ggtgggctcg tcgaggaaga ggaggtcggg gtcgttcacg agggcgatgc
     1441 cgaggagcat ccgctggcgc tggccccccg agagcttgcg gttgtccctg tcgatgaact
     1501 cctcgagcga gcaggcctcg atgatgtcgg ccatgggcct cgggttcggg tagagcttgg
     1561 cgaagagctc gagggtctcc cgcaccgtga ggaactcctg gagcgaggtg tcctggaact
     1621 ggatcccgat gttctccttg aaggagcggg agaccggggc gccgcggtag aggatctgcc
     1681 ccgagtcggg ggcgtggatc gtctcgatca cctcgatggc cgtggtcttg ccagccccgt
     1741 tggggccgag gatcccgaag caggagccct cgcgcacgga gaagctgatg ccgtccacgg
     1801 ccttgacctt gccgtaggcc ttgcgcaggt cgcggacctc gaggatggcg cttgtgtcag
     1861 atgcttggct cattgcggtt caaggtactc cctcgaaccg cattgaggca acgtgaatgc
     1921 gccgcccgtg gcgaggagcc tactccgtcc tgaaggccgc gaggctctcc gagatcctcg
     1981 tcgtgaccgc gtcgatctcg cggctcttgc ggcccgagtc ctcggtgcag gagccgaccg
     2041 tcctcacctt ctcggtcatg tcggcggtga ccgtcctcgt ctcggcgaag agctccttca
     2101 cgcccgccat ggcctggccc acggactcgg tctcggcgct gatcgtgagc gagcttcccg
     2161 agacccggct cgagatctcg cgcagggtcg agaccgactc gaggatctgg gtcccgccca
     2221 ccttgagctc gccgacgctc cgctcgatct cttcgaagga ggagacgacc tcggcgatct
     2281 cggtgcgcat gccctggaag gct
//
LOCUS       DASSQV010000148         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2151046, whole genome shotgun sequence.
ACCESSION   DASSQV010000148 DASSQV010000000
VERSION     DASSQV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151046"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFL04_02605"
     CDS             <1..441
                     /locus_tag="VFL04_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7838624.1"
                     /translation="PDAVSSASKAGRTIPYSGDVAYLNLYLLTPKGAYLYQPDKSALK
                     QVSAKDERAAITSEFIKTSPFMILYVADTAKAPSFLKGNKAMLREMMNADAGFAAENV
                     GLVASSLKLGSIIMYNIKPADAAGLLGMGKEEIPLFVMQFGYTQ"
     gene            500..1432
                     /locus_tag="VFL04_02610"
     CDS             500..1432
                     /locus_tag="VFL04_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HET7838625.1"
                     /translation="MKLDRLLSIVILLLNHESMNAAELARRFEVSVRTIYRDLDAVNA
                     AGIPVVASPGPGGGFGIDPSYTIDRRLLGFDDLRAIIAALKGVNTGLEDRSIASALEK
                     ITSLAPRGRAAEFLEDRVVIDLFPWGARKEERRLVRLLEPAIAERRLVSFTYSSYGRK
                     REKRLVEPMTLVFKAYAWYLWAWCRLRGGYRLFKLSRIRDLELRLDRFKRRAEPYPRE
                     TELEPPALQELSLRFDPACAAQAEEWFGGEEGEREAGGGLRLRLSLPAGDWIVRTLLG
                     FGPGLEVLAPESLRLDLARAAQAIAAANDRPNRD"
     gene            1529..2176
                     /locus_tag="VFL04_02615"
     CDS             1529..2176
                     /locus_tag="VFL04_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838626.1"
                     /translation="MKTQAIVLLGLVGLAVLGLLVWLFFLKGPDLRPYLGLKEPRART
                     MGPERVLEVEFSGPADTAIPQAFGQLFKTYYSLKGSPKGPAMKPPKARYRIPADSGPG
                     AEERLREFKNHPWTGSVAIPLPDSVELPAEKAGAGGAVARVGTWDYGEVAEILHLGSY
                     DTEPATIQRLEDFMKAKGLKPIGDHEEEYLKGPGMPLVSPKDYWTIIRYRVAKAD"
BASE COUNT          378 a          768 c          704 g          341 t
ORIGIN      
        1 cccgacgccg tctcctcggc ctccaaggcc gggcggacga ttccctactc gggcgacgtc
       61 gcctacctca acctctatct cctcaccccg aagggcgcct acctctacca gccggacaaa
      121 tcggcgctca agcaggtgtc ggcgaaggac gagagggcgg cgatcacgag cgagttcata
      181 aagacctcgc ccttcatgat cctgtacgtg gccgacaccg ccaaggctcc gtccttcctc
      241 aagggcaaca aggccatgct ccgcgagatg atgaacgccg acgcgggctt cgccgcagag
      301 aacgtgggcc tggtcgcctc gagcctcaag ctcggctcca tcatcatgta caacatcaaa
      361 cccgccgatg cggcaggcct cctcggcatg ggcaaggagg agatccccct cttcgtgatg
      421 cagttcggct acacgcagta ggccagactc gcccttgatc ggcgctccca ccggggctag
      481 tctcctggga gggagcgcca tgaagctcga ccgcctcttg tccatcgtca tcctcctgct
      541 caaccacgag agcatgaacg ccgccgagct cgcgcggcgt ttcgaggtga gcgtgcggac
      601 gatctaccgc gatctcgacg cggtcaacgc ggccggcatc cccgtggtgg ccagccccgg
      661 gccagggggc ggtttcggca tcgatccctc ctacaccatc gacagacggc tcctcggctt
      721 cgacgacctc agggcgatca tcgccgccct caagggcgtg aacacgggcc tcgaggacag
      781 gtccatcgcc tcggccctcg agaagatcac gagcctggcg ccccggggga gggcggccga
      841 gttcctcgag gacagggtcg tcatcgacct tttcccctgg ggggcgagga aggaggagag
      901 gcggctggtc aggctcctcg agcccgccat cgccgagcgc cgcctcgtct cgttcaccta
      961 ctcgtcctat ggcaggaaac gggagaaacg gctggtcgag cccatgaccc tcgtgttcaa
     1021 ggcctatgcc tggtacctct gggcctggtg ccgcctcagg ggcggctacc gcctcttcaa
     1081 gctctcccgg atcagggatc tcgagctccg ccttgaccgg ttcaagcgga gggccgagcc
     1141 ctatccccgg gagaccgagc tcgagccccc cgccttgcag gagctctccc tgcgtttcga
     1201 tccggcctgc gcggcccagg ccgaggaatg gttcgggggg gaggagggcg agagggaggc
     1261 cgggggaggc ctcaggctca ggctcagcct tcccgcgggc gactggatag tgaggaccct
     1321 gctcggcttc gggcccgggc tcgaggtcct cgcccccgag agcctccgcc tcgacctggc
     1381 cagggccgct caggcgatcg ccgccgccaa cgacaggcca aaccgggact gagccgggac
     1441 gcggattttc cgtaaaaccc tgacaggggg ctgtcacgcc ccgggtctag gatcgaggca
     1501 tagaacgttc cacccaagga gggatcacat gaaaacgcag gcgatcgtcc ttctcggcct
     1561 cgtcggcctc gccgtcctcg gtttgttggt ctggctcttc ttcctgaagg gccccgatct
     1621 gcgcccctat cttggcctga aggagccccg ggcccgcacg atgggacctg agcgcgtcct
     1681 tgaggtcgag ttctcgggcc cggccgacac ggccatcccc caggccttcg gccagctctt
     1741 caagacctac tacagcctca agggcagccc caagggccct gcgatgaagc ctccgaaggc
     1801 ccgctaccgc atccccgccg actcggggcc gggggccgag gagaggctca gggagttcaa
     1861 gaaccatccc tggacgggct cggtggccat tccccttccc gactcggtcg agctgcccgc
     1921 cgagaaggcc ggagccggcg gcgccgtcgc cagggtcggc acctgggact acggcgaggt
     1981 ggccgagatc ctccatctgg gcagctacga caccgagccc gcgaccatcc agaggctcga
     2041 ggatttcatg aaggccaagg gactcaagcc gatcggagac cacgaggagg agtacctgaa
     2101 ggggcccggc atgcccctgg tctcgcccaa ggactattgg accatcatcc gctacagggt
     2161 ggccaaggcg gactagggaa ggccccgccg g
//
LOCUS       DASSQV010000149         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1528778, whole genome shotgun sequence.
ACCESSION   DASSQV010000149 DASSQV010000000
VERSION     DASSQV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528778"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VFL04_02620"
     CDS             complement(<1..911)
                     /locus_tag="VFL04_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET7838627.1"
                     /translation="MQPPSTSTPSGEKSTPEVQRGRGALGRAWRALAVLLVAASSLLL
                     GGAAAPSHDFWAAGKDEALASSLLERMGDVEVLGQLLMLAYPGSDPPPLLFDWIEARG
                     LGGVKIFGWNAEDTDRLAVSVAALQRRALSSGEGIPLLLATDQEGGWIRHVKGATTVT
                     PGNMAIGASGRPYDAYRSAFLIGRELAALGINMNFAPAVDLATRPKSQIIGPRAFSSD
                     PMEAAALGAAFARGLAEAGIIATAKHFPGHGDTEQDSHGVLPVIMDDEKTLWNRELIP
                     YRMLAAEGIPAVMSGHLSFPLITGDGSP"
     gene            complement(862..1797)
                     /gene="trxB"
                     /locus_tag="VFL04_02625"
     CDS             complement(862..1797)
                     /gene="trxB"
                     /locus_tag="VFL04_02625"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET7838628.1"
                     /translation="MDTQYEFVVIGAGAAGLAAAQYGARANLRTLLIEEMAPGGQALL
                     IDGLENYPGIVGPLSGYELSEAMRVQAEAFGVEFLTASVTALTKEGERFIVETTEGRV
                     EALAVLLATGAKHRHIGVPGEEEFSGRGVSYCATCDGPFFKNRRMLVVGGGDAACDEA
                     MFLSKLADSIVMVHRKDRFRAQRALAERVLKNPKIEVRFNTVVKEIQGDKKVQTVQLE
                     DLAEGRSYSEEVAAVFVFVGSIPQTSLAGTAVKDEAGYLVTNELMETSVSGLFAAGDA
                     RVTPFRQLVTACADGAIAAHAAAKYIDALRGEVYA"
BASE COUNT          285 a          663 c          744 g          328 t
ORIGIN      
        1 ggcgagccgt cgccggtgat gagggggaag ctcaggtggc cgctcatgac ggccgggatg
       61 ccctcggcgg cgagcatgcg gtaggggatg agctcgcggt tccagagggt cttctcgtcg
      121 tccatgatga cggggaggac gccgtgggag tcctgctccg tgtccccgtg gccggggaag
      181 tgcttggccg tggcgatgat ccccgcctcg gcgaggcccc tggcgaaggc tgccccgagg
      241 gcagccgcct ccatgggatc ggaggagaag gcccgcggcc cgatgatctg gctcttgggc
      301 cgggtcgcga ggtcgactgc gggggcgaag ttcatgttta tgccgagggc ggccagctcg
      361 cgtccgatga ggaaggccga gcggtaggcg tcatagggcc tccccgaggc cccgatggcc
      421 atgttgcccg gggtgacggt cgtggcgccc ttcacgtggc ggatccagcc cccctcctgg
      481 tcggtggcga ggaggagggg gatgccctcc cccgaggaca gggccctccg ctggagggcg
      541 gctaccgaga cggcgagcct gtcggtgtcc tcggcgttcc agccgaagat cttcaccccg
      601 ccgaggcccc tcgcctcgat ccagtcgaag agcagggggg gcggatcgct gcccggatag
      661 gcgagcatga ggagctggcc gaggacctcg acgtcgccca tcctctcgag gagggagctg
      721 gccaaggcct cgtccttccc ggccgcccag aaatcgtggc ttggcgccgc cgcccctccg
      781 aggaggagcg acgaggcggc gacgaggagg acggccaggg cgcgccatgc gcggcccagg
      841 gcccccctcc ccctctggac ctcaggcgta gacttctccc ctgagggcgt cgatgtactt
      901 ggcggctgca tgggcggcga tggccccgtc ggcgcaggcg gtgacgagct ggcggaaggg
      961 cgtgaccctg gcgtcgccgg cggcgaagag gccggagacg ctcgtctcca tgagctcgtt
     1021 cgtcacgagg tagccggcct cgtccttcac cgcggttccg gcgagcgagg tctgggggat
     1081 cgagccgacg aagacgaaga cggcggcgac ttcctcggag tagctcctgc cctcggcgag
     1141 gtcctcgagc tggacggtct ggaccttctt gtccccctgg atctccttga ccacggtgtt
     1201 gaagcggacc tcgatcttgg ggttcttgag gacccgctcg gcgagggcgc gctgggccct
     1261 gaagcggtcc ttgcggtgga ccatgacgat ggagtcggcc agcttggaga ggaacatggc
     1321 ctcgtcgcag gcggcgtcgc ccccgcccac gacgagcatg cggcggttct tgaagaaggg
     1381 gccgtcgcag gtcgcgcagt aggagacccc cctgcccgag aactcctcct cgccgggcac
     1441 gccgatgtgg cggtgttttg cccccgtggc gaggaggacg gccagggcct cgaccctgcc
     1501 ctcggtggtc tcgacgatga aacgctcgcc ctccttggtc agggcggtca ccgaagcggt
     1561 gaggaactcg acgccgaagg cctcggcctg gacccgcatc gcctcgctga gctcgtagcc
     1621 gctcaggggg ccgacaattc cggggtagtt ctccagccca tcgatgagga gggcctggcc
     1681 tccgggggcc atctcctcta tcagcagggt gcgcaggttg gccctggccc cgtattgggc
     1741 ggccgcgagc ccggccgcgc cggctcctat gacgacgaat tcgtactggg tatccatgag
     1801 gctcctcgat agctctgtgc gagatgcttg gctaatatac atcgggcggg ttatttttga
     1861 gaacacggta gacatgatga gccccacgac aaagcgctgc ctccttctgg tgccggccct
     1921 gctggccggc ctcgtgatgg ctcccgcccc cggggcggcc cagggggctg cccaggggct
     1981 ggcccccgcc tacgggcccg gtttcttcag gccagacccg
//
LOCUS       DASSQV010000150         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1528853, whole genome shotgun sequence.
ACCESSION   DASSQV010000150 DASSQV010000000
VERSION     DASSQV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528853"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VFL04_02630"
     CDS             complement(<1..689)
                     /locus_tag="VFL04_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838629.1"
                     /translation="MSIRMSGRAVVLCVAIGLSSCQSFQIPKDYLPDAKLVFLTQNPG
                     LTPISYKPEAADPYVFAPDTSPLDQRPAFLSGLSWAGLLRPDFKADSRFYRSVEAYFG
                     DILKDQGIKELREALDAAKIVQTPALPPDRGDQIKTYSNGGLYQYPGFEGAQITVLEG
                     GYYEVLFPDKGYFRHQADGTYERVDGAGKLVFAFYPKRSQIVSTMGGLVYTSDPHFRE
                     LKGPTGTLSYT"
     gene            884..1324
                     /locus_tag="VFL04_02635"
     CDS             884..1324
                     /locus_tag="VFL04_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838630.1"
                     /translation="MRKSMVAVIISIILTLTGCMTTEEFREMQQRNARIEKERFTVNA
                     KPGSIVIKTFEFDRILLFSTESLEGLKADIADAVLNGVDRVNVSLVAQDKNPPAIRLY
                     LQLSPNAPLGEQAITIVLRYRKLDATTKKSGDGEQEKTVYLNVQ"
     gene            complement(1350..>3250)
                     /locus_tag="VFL04_02640"
     CDS             complement(1350..>3250)
                     /locus_tag="VFL04_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET7838631.1"
                     /translation="AATLAVLPNSPSFASPGRNREALRAKRDRLLRTLATRGSLSPWE
                     LGLALAEPLPPSSFPLPSHAPQLVARAVLAGAGQRGGPPARIETTLDLGVQTRAGELV
                     RRRLDRLAGGGVRNAACVVARIDSGAVLAYLSVSADRAGEGAEAPAQDSRTGASMDLV
                     RATRSSGSLLKPFLYAAAIEAGELGPKSLLPDLPTRYGSYEPENNLQTYAGAVRADEA
                     LARSLNVPAVRLLRSYGVERFRSLLVSTGVSTMDRGADDYGLTLILGGAETNLWEMAG
                     RYAALARTAGWSGRGPSAAPKGRQYFDLGLTRAELDSRALREDPFSPGSAWLTLMALT
                     RVARPEDEAAWEDYASARNIAWKTGTSFGNRDAWAIGLDGEYVVGIWVGNATGEGRPS
                     LKGSAAAAPILFDVLNSLRPGLGSESRGARSEARGAEPSPSPTGAKVGPALREVAVCA
                     DSGWAAGPDCPRLDTCLVPAGAKALPVCPYCRSVALSLDGAFRVRAQDLAPGEFRMEK
                     RFVLPSSMEYYYRRGHLEYRPLPPWRGGSAGGSAYDSMAITAPAAGSQIYIPVEIDGR
                     PGATVFAATHRDPEAVLYWQLDGIYLGSTRTEHRMAARPGLGEHILVVVDQAGRELSC
                     RFFVLSRK"
BASE COUNT          527 a         1051 c         1199 g          473 t
ORIGIN      
        1 tcggtgtagc tcaaggtccc ggtggggccc ttcagctcgc ggaaatgggg gtccgaggtg
       61 tagacgaggc cgcccatcgt gctgacgatc tgcgagcgct tggggtagaa ggcgaaaacc
      121 agcttcccgg ccccgtccac ccgttcataa gtcccgtcgg cctggtggcg gaaatagccc
      181 ttgtcgggga acaggacctc atagtatccg ccctcgagga ccgtgatctg ggctccctcg
      241 aagccggggt actggtagag cccgccattg ctgtaggtct tgatctggtc gccccggtcc
      301 ggagggaggg ctggggtctg gacgatcttc gcggcgtcga gggcctcgcg gagctccttt
      361 atcccctgat ccttgaggat atccccgaag taggcctcga cggagcggta gaaacgcgag
      421 tcggccttga aatcgggcct gaggaggccg gcccagctca ggcccgagag gaaggcgggc
      481 ctctggtcga gggggctcgt atcgggggca aacacgtagg gatcggcggc ctcaggcttg
      541 taggagatcg gggtgaggcc gggattctgg gtcaggaaga ccaacttagc atcgggcagg
      601 tagtccttgg ggatctggaa gctctggcag gacgaaagac cgatggcgac gcatagaacc
      661 acagctcgac cggacatccg gatgctcacg tttgcctccc attttgtgct tgttcttgcc
      721 gactgcgcga tcaattctag gccgggtttc ccgggaccgc aagggcgcgg gattctggcc
      781 ccgatgcgac attgcgcgat gagcgaacga ggacaccatg gcgcaggagg cggagctagg
      841 ctagactcgg gaagtcgacg aggccaatcc aaaggagagc atcatgagaa aatccatggt
      901 cgcggtcatc atctcgatca tccttaccct gaccggctgc atgaccacgg aggaattccg
      961 cgagatgcag cagaggaacg cgaggatcga gaaggagagg ttcacggtca acgcgaaacc
     1021 cgggagcatc gtgatcaaga ccttcgagtt cgaccgcata ctcctgttct ccaccgagag
     1081 cctcgagggg ctgaaggccg acatcgccga tgccgtgctc aatggggtcg acagggtcaa
     1141 tgtgagcctt gtggctcagg acaagaaccc gcccgcgatc cggctctatc tccagctgag
     1201 cccgaacgcg ccgctcgggg agcaggcgat cacgatcgtg ctccggtaca ggaagctcga
     1261 cgccaccacc aagaagtcgg gcgatggaga gcaggagaag acggtctatc tgaacgtaca
     1321 gtgagcgggg ggtgacgaag ccgccccccc tacttccggc tcaggacgaa gaacctgcag
     1381 gacagctccc tgcccgcctg atcgaccacc acgaggatgt gttcgccgag gccgggcctc
     1441 gcggccatcc ggtgctccgt ccgggtcgag ccgaggtaga tgccgtcgag ctgccagtac
     1501 agcacggcct cggggtcgcg gtgggtcgcg gcgaagaccg tcgcccccgg cctgccgtcg
     1561 atctccacgg ggatgtagat ctggcttccc gccgcggggg ccgtgatcgc catgctgtcg
     1621 taggctgagc cgccggcgct gcctccccgc cagggcggca ggggccggta ctcgagatgg
     1681 ccccggcggt agtagtactc catcgaggag ggcaggacga aacgcttctc catccggaac
     1741 tcgccgggcg cgaggtcctg ggcccgcacg cggaaggcac cgtccaggct cagggcgacg
     1801 ctccggcagt aggggcagac gggcagggcc ttggcccccg cgggcacgag acaggtatcc
     1861 agacggggac agtccggccc ggcggcccag cccgagtcgg cgcagaccgc gacctccctg
     1921 agggctgggc cgaccttcgc gccggtcggc gaggggctgg gctccgcgcc gcgcgcctcg
     1981 ctcctcgccc cgcggctctc cgaacccagg ccgggcctca gactgttcag cacgtcgaag
     2041 aggatcgggg ccgcggcggc cgagcccttg agcgatggcc tgccctcccc cgtcgcattg
     2101 cccacccaga tccccaccac gtactctcca tcaaggccga tggcccaggc gtcgcggttg
     2161 ccgaagctgg tccccgtctt ccaggcgatg ttccgcgccg acgcgtagtc ctcccaggcg
     2221 gcctcgtcct cgggccgggc gacccgggtc agggccatga gggtgagcca ggccgagccc
     2281 ggggagaagg ggtcctccct cagggcgcgg gagtcgagct cggccctggt gaggccgagg
     2341 tcgaagtact gccgcccctt cggcgcggcg ctggggcccc tgcccgacca gcccgccgtc
     2401 ctcgcgaggg cggcgtagcg gcccgccatc tcccagaggt tcgtctcggc cccgccgagg
     2461 atcagggtga gcccgtagtc gtcggccccg cggtccatcg tgctcacgcc cgtggatacg
     2521 aggagggagc ggaagcgctc cacaccgtag gaccggagca agcgcacggc gggaacgttg
     2581 agcgaacgag cgagggcctc gtcggcccgc acggccccgg cgtaggtctg caggttgttc
     2641 tcgggctcgt aggagccgta gcgggtcggc aggtcgggca ggaggctctt ggggccgagc
     2701 tcgccggcct cgatcgcggc cgcgtagagg aagggcttga ggagggagcc cgagctccgc
     2761 gtggcgcgga ccaggtccat ggaggccccg gtcctgctgt cctgggccgg ggcttcggcc
     2821 ccttcccctg cccggtcggc cgagacgctg aggtaggcca ggacggcgcc cgagtcgatg
     2881 cgcgccacca cgcaggcggc gttgcgcacc ccgcccccgg ccagacggtc gagccgcctc
     2941 cgcacgagct cgcccgccct cgtctggacg ccgaggtcga gggtggtttc gatccgggcg
     3001 ggggggccgc cgcgttggcc ggccccggcc aggacggcgc gggcgacgag ctggggcgcg
     3061 tgggacggga ggggaaagga ggagggaggg aggggctcgg cgagggcgag cccgagctcc
     3121 cagggggaga gcgagccccg cgtggcgagg gtccgcagga gacggtcgcg cttggcgcgg
     3181 agggcctccc tgttgcggcc ggggctcgcg aaggagggcg agttgggcag gacggcgagg
     3241 gtcgctgcct
//
LOCUS       DASSQV010000151         4910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_535236, whole genome shotgun sequence.
ACCESSION   DASSQV010000151 DASSQV010000000
VERSION     DASSQV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535236"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VFL04_02645"
     CDS             complement(<1..1218)
                     /locus_tag="VFL04_02645"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET7838632.1"
                     /translation="MSPPLLELRSLRKTFSGSFALEDICLSLEGGQVNVLIGQNGSGK
                     SALMKLVCGQYRRDSGAVLLGGQEVDFPSMHESRKAGIAYQQQDPLLYDNLSVAENVF
                     FHRIPRRRMLPRIIDPLMLQKECAELFARLGVGIEPQALIAELGYTERLLVMAAQTCV
                     ADARLVILDEPTAGMADPEREILFNIVREIRGRGIGVVYISHKLDEIPILGDLVTVIH
                     EGRISGSLPVGEADRETLIRLMTGRSCTERYPRLNVEPGARVLSVEHLQSGGVLKDVS
                     FALKRGEILGITGLMGSGRTRLANCLFGQTRPSGGSITVDGRKAEFGHPAEALARGLA
                     LIPEDREQNAVFHFQDIVRNMSLASLLRFKGGSGLDTRYMHELTDEYIRSLGILPGHP
                     DDLMRSYSGGNQQK"
     gene            complement(1215..2012)
                     /locus_tag="VFL04_02650"
     CDS             complement(1215..2012)
                     /locus_tag="VFL04_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET7838633.1"
                     /translation="MFAVERINLIRSYLREHGKLDVHSISKMLNVSEVTIRRDLEKLE
                     AEGSLTRLHGGAILAEVEEEAKAPAPTEEERRAEEDREEIARLAFLMVKDGDVIMLTN
                     GAIALRLARKLSARSGVTVLTNDIPVAMEMASQAANKAVLLGGNPDAETGALFGSLAE
                     ANVQKFFVNKIFVQVDGISEQLQLTVSSEEKAALIKEALACAEDRIILCEAGRFSHNA
                     FYRLGPLSLAGKVVTNTSLGEAYKARIFALEIQLFTSINAFEGGGQG"
     gene            2225..3298
                     /gene="chvE"
                     /locus_tag="VFL04_02655"
     CDS             2225..3298
                     /gene="chvE"
                     /locus_tag="VFL04_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7838634.1"
                     /translation="MNRFGKSVLLVLAICILAGGSLVAQAVKTVGIAMPTQSSARWIA
                     DGGNMKKVLEARGYKVDLQYAEDNIDAQVAQVENMITKGDSVLVIAAIDGAALFNVLE
                     KAHAAKVPVISYDRLIVNSPWVSYYATFDNFKVGVLQGGYIVKALGLDKGQKGPFNIE
                     LFAGSPDDTNAGYFFNGAMSQLGPYIKSGVLVVKSGQTEFAKVATLRWDGAVAQKRMD
                     DILTSKYTDAHVDAVLSPYDGLSIGILSALKSVGYGSAKKPLPVVTGQDAEIPSVKSI
                     LAGEQTSTVFKDTRTLAKRAADMVDSVLSGKPAEINDTKTYNNGHHIIPSYLCEPISV
                     DKTNIKAALVDIGYYTAAQIGLK"
     gene            3497..>4910
                     /locus_tag="VFL04_02660"
     CDS             3497..>4910
                     /locus_tag="VFL04_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7838635.1"
                     /translation="MSDTILEMKGITKTFAGVKALDEVSLKVARDEIHALVGENGAGK
                     STLMKILSGVYPHGDYTGSIHYEGGECRFRSIADSEKLGIVIIHQELALIPYLSIAEN
                     IFLGNERARAGVIDWHRTTAAAGELLAKVGLHENPNTLITDIGVGKQQLVEIAKALAK
                     RVRLLILDEPTAALNDEESSKLLALLLELKKQGVTSILISHKLEEIARVSDAITIIRD
                     GKVIETLDNASHQVDQDSIIKSMVGREIVDRFPRRASEIGEVVFRVEDWSVYHPQYED
                     RRVISNISLNVRRGEVVGLAGLMGAGRTELAMSLFGRSYGRRHSGRIFKNEVEIHPHS
                     VSESIDQGIAYVTEDRKNFGLVLIEDIKSNVSLASLPKVSRGQVINQGLENQAASEQQ
                     KRMRIRCTGIDQVVESLSGGNQQKVVLSKWIMAEPEVLILDEPTRGIDVGAKYEIYTI
                     INRLASEGKSIIMISSEMPEL"
BASE COUNT          884 a         1645 c         1568 g          813 t
ORIGIN      
        1 cttctgctgg ttcccgcccg agtagctcct catgaggtcg tcggggtgtc cgggcaggat
       61 gcccaggctg cgtatgtatt cgtcggtgag ctcgtgcatg taacgcgtgt ccaggcccga
      121 gccccccttg aaccggagca gggaggcgag ggacatgttc ctgacgatgt cctggaagtg
      181 gaagacggcg ttctgctcgc ggtcctcggg gatcagggcg aggccacggg cgagggcctc
      241 ggcggggtgg ccgaactcgg ccttccgccc atccaccgtg atcgagccgc cggagggcct
      301 cgtctggccg aaaaggcagt tcgcgagcct cgtcctcccc gagcccatga gcccggtgat
      361 gccgaggatc tcgccccgct tgagggcgaa gctcacgtcc ttgagcaccc cgccgctctg
      421 caggtgctcg accgagagga ccctcgcgcc cggctccacg ttgagccgag ggtagcgctc
      481 ggtgcagctg cggcccgtca tgaggcggat cagggtctcc ctgtcggcct cgccgacggg
      541 caggctcccg ctgatcctgc cctcgtggat gacggtgacc aggtccccga ggatggggat
      601 ctcgtcgagc ttgtgggaga tgtagaccac gccgatgccc cggcccctga tctcgcggac
      661 gatgttgaac agtatctcgc gctcggggtc ggccatcccg gctgtgggct cgtcgaggat
      721 cacgagcctc gcgtcggcga cgcaggtctg ggcggccatg accaggaggc gctcggtgta
      781 gccgagctcc gcgatgaggg cctggggctc gatgcccacg ccgagcctgg cgaagagctc
      841 ggcgcactct ttctggagca tgagggggtc gatgatccga ggcagcatgc ggcggcgggg
      901 gatgcgatgg aagaagacgt tctccgcgac gctcaggttg tcgtagagga gggggtcctg
      961 ctgctggtag gcgatgcccg ccttcctcga ttcgtgcatc gacgggaagt cgacctcctg
     1021 gcccccgagg aggacggcgc cggagtccct gcggtactgg ccgcacacga gcttcatgag
     1081 ggccgacttg cccgagccgt tctggccgat gaggacgttc acctgcccgc cctcaaggct
     1141 gaggcaaatg tcctcgaggg cgaagctccc ggagaaggtc ttccggaggg agcgcagctc
     1201 gaggagggga gggctcatcc ctggccccct ccctcgaagg cgttgatcga ggtgaagagc
     1261 tggatctcca gggcgaagat gcgcgccttg taggcctcgc cgagggaggt gttggtcacc
     1321 accttgcccg ccagggagag ggggccgagg cggtagaagg cgttgtggga gaaacgcccg
     1381 gcctcgcaga ggatgatcct gtcctcggcg caggcgaggg cctccttgat gagggccgcc
     1441 ttttcctcgc tcgagacggt cagctggagc tgctcgctga tcccgtcgac ctggacgaag
     1501 atcttgttga cgaagaactt ctggacgttg gcctcggcca ggctgccgaa gagggccccg
     1561 gtctcggcgt cggggttgcc gccgagtagg actgccttgt tggcggcctg ggaggccatc
     1621 tccatggcca ccgggatgtc gttggtgagg accgtgacgc cgctgcgcgc cgagagcttc
     1681 cgcgcgaggc ggagggcgat ggccccgttc gtgagcatga tgacgtcgcc gtccttgacc
     1741 atgaggaagg ccagccgggc gatctcctcc ctgtcctcct ccgcccgcct ctcctcctcg
     1801 gtgggcgcgg gggccttggc ctcctcctcg acctcggcca ggatggcccc gccgtggagc
     1861 cgggtcaggg agccctcggc ctcgagcttc tcgaggtcgc ggcgtatggt gacctcggag
     1921 acattgagca tcttgctgat ggaatggacg tcgagcttgc cgtgctcgcg gaggtagctt
     1981 cgtatgaggt tgatccgttc gactgcgaac atcgggcttc agggctcctg tcggtggccc
     2041 attgtagatg tcctcgtccg aaacctcaag tttttaattg acggttcggg aaagcggtgc
     2101 taggctccga actgaaacga aaccaaacga acaaaaaggc acagaattag ggtgtccatt
     2161 tgatcgttta gtttcttgtg ggtttgtttc ccacagcacc acattcttta aggagcgtcc
     2221 acgaatgaat cgcttcggaa agtccgtcct cctcgtcctc gcgatctgca tcctcgccgg
     2281 cggcagcctc gtggcccagg cggtcaagac ggtcggcatc gccatgccca cccagtcctc
     2341 ggcccgctgg atcgccgacg gcggcaacat gaagaaggtc ctcgaggccc gcggctacaa
     2401 ggtcgacctc cagtacgccg aggacaacat cgacgcccag gtcgcccagg tcgagaacat
     2461 gatcaccaag ggcgactccg tcctcgtgat cgccgccatc gacggcgcgg ccctgttcaa
     2521 cgtcctcgag aaggcccacg cggccaaggt ccccgtcatc tcctacgacc gcctcatcgt
     2581 caacagcccc tgggtctcct actacgccac cttcgacaac ttcaaggtcg gcgtcctcca
     2641 gggcggctac atcgtcaagg ccctcggcct ggacaagggc cagaagggcc ccttcaacat
     2701 cgagctcttc gccggttccc ccgacgacac caacgccggc tacttcttca acggcgcaat
     2761 gtcccagctc gggccctaca tcaagtccgg cgtcctcgtc gtgaagtccg gccagaccga
     2821 gttcgccaag gtcgccaccc tccgctggga cggcgccgtg gcccagaagc gcatggacga
     2881 catcctcacc tccaagtaca ccgacgccca cgtcgacgcc gtcctctccc cctacgacgg
     2941 cctctccatc ggcatcctct ccgcgctaaa gtccgtcggc tacggcagcg cgaagaagcc
     3001 cctccccgtc gtgaccggtc aggacgccga gatcccctcg gtcaagtcca tcctcgccgg
     3061 cgagcagacc tcgacggtgt tcaaggatac ccgcaccctc gccaagcgcg ccgccgacat
     3121 ggtcgactcc gtcctctccg gcaagcccgc cgagatcaac gacaccaaga cctacaacaa
     3181 cggccaccac atcatcccct cctacctctg cgagcccatc agcgtcgaca agacgaacat
     3241 caaggccgcc ctggtggaca tcggctacta caccgccgcc cagatcggcc tgaagtagac
     3301 atcctggccc cagccaggcc gaggtaaaaa gagcatcgga agcaccgagc cacgggagcg
     3361 cggagggaca cggagcggca ggaagggaaa gggggtgggg gaggcgatgg cgccatctcg
     3421 cccgtcttcc ctccctggcc cgctccgtgt actctgtgcc tccgtgcttg catttcgttc
     3481 gcgcaaaggc ggagacatgt cggacactat cctggagatg aaggggatca cgaagacctt
     3541 cgcgggggtg aaggccctcg acgaggtcag cctgaaggtg gcccgcgacg agatccacgc
     3601 cctcgtgggc gagaacgggg cgggcaagtc caccctcatg aagatcctga gcggggtcta
     3661 cccgcacggg gactacacgg gctcgatcca ctacgaaggc ggcgaatgcc gcttccggag
     3721 catcgccgac agcgagaagc tggggatcgt catcatccac caggagctgg ccctcatccc
     3781 ctacctgtcg atcgccgaga acatcttcct cggcaacgag agggcgaggg ccggggtcat
     3841 cgactggcac cggaccacgg ccgcggccgg cgagctcctg gccaaggtcg ggctccacga
     3901 gaacccgaac accctcatca ccgacatagg cgtcggcaag cagcagctgg tcgagatagc
     3961 caaggccctc gccaagaggg tgaggctcct catcctcgac gagcccacgg ccgccctgaa
     4021 cgacgaggag tcctcgaagc tcctggccct cctcctcgag ctcaagaagc agggcgtcac
     4081 ctcgatcctc atctcgcaca agctcgagga gatcgcccgc gtctcggacg ccatcaccat
     4141 aatccgcgac ggcaaggtca tcgagaccct cgacaatgcc tcccaccagg tcgaccagga
     4201 cagcatcatc aagagcatgg tcggccgcga gatcgttgac cgctttcccc ggcgcgcctc
     4261 ggagatcggc gaggtcgtct tccgcgtcga ggactggagc gtctaccacc cccagtacga
     4321 ggaccgcagg gtcatctcga acatctcgct gaacgtccgc cgcggcgagg tcgtcggcct
     4381 cgccggcctc atgggcgcgg gccgcaccga gctcgccatg agcctcttcg gccgctccta
     4441 cggcaggcgc cactccggga ggatcttcaa gaacgaggtc gagatccacc cccactcggt
     4501 gagcgagtcg atcgaccagg gcatcgccta cgtgaccgag gaccgcaaga acttcggcct
     4561 cgtgctcatc gaggacatca agtccaacgt gtccctggcc agcctcccga aggtgagcag
     4621 ggggcaggtg atcaaccagg gcctcgagaa ccaggcggcc tcggagcagc agaagcggat
     4681 gcggatcagg tgcacgggca tcgaccaggt ggtcgagagc ctctcgggcg gcaatcagca
     4741 gaaggtagtc ctctcgaaat ggatcatggc cgagcccgag gtcctcatcc ttgacgagcc
     4801 gacccgcggc atcgacgtgg gcgcgaagta cgagatctac accatcatca accgcctcgc
     4861 ctcggagggg aagtcgatca tcatgatctc ctccgagatg cccgagctct
//
LOCUS       DASSQV010000152         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1529323, whole genome shotgun sequence.
ACCESSION   DASSQV010000152 DASSQV010000000
VERSION     DASSQV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529323"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..413
                     /locus_tag="VFL04_02665"
     CDS             114..413
                     /locus_tag="VFL04_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HET7838636.1"
                     /translation="MLKVSETREPAKPTTKSYLVIWAALVLLTGLTVTMASLNLGRAA
                     IFSVLAIAALKSSLVLFYFMHLRYEGRLLIRLLVPIVLATLAIFIGLTYTDILYR"
     gene            463..1377
                     /gene="coxB"
                     /locus_tag="VFL04_02670"
     CDS             463..1377
                     /gene="coxB"
                     /locus_tag="VFL04_02670"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7838637.1"
                     /translation="MTSTASVNEAFYFITAVSALLFFGIVFLMILFLVRYRRSRNPEA
                     TEIPGNLWLEVTWIVVPTVIVLVMFVYGLTGFTLQRTKVAGSLEVEVHTRQWSFLFEY
                     PSGIKSPDLVAPSGRDVRITLESDDVIHAFYLPAYRVQIDAVPGMKTYANFRTGAVGS
                     FDILCAQYCGTLHSSMLAKLYVLPPADYDRWTRGEKVQLAGKNLPPTAATGLELLRQR
                     SCLACHSTDGSPLAGPTFKGLFGSTVEVMTAGSKRSVVADEAFIRKSIVDPAADIAVG
                     FQNIMPSGKDSFGKDDLDEAVEAIKALK"
     gene            complement(1469..>2015)
                     /locus_tag="VFL04_02675"
     CDS             complement(1469..>2015)
                     /locus_tag="VFL04_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7838638.1"
                     /translation="QIYYSLQARDAEYELVPLTLDQGLGILVWSPLAGGFLSGKYQGS
                     SPPPEGTRFAEAGRFVPFDADYGWRVVEAQRKVAARLGVSPARVALAWLLGRPAITSV
                     IAAARSLEHLEDNLAASDLDLSDEDRRELEHASNPGIPYPKWMVLQHDVAEDPRLRTL
                     HPELFTNGGTWEDLRGKAWRG"
BASE COUNT          311 a          730 c          633 g          341 t
ORIGIN      
        1 gagaacgcca ccctcttctg gcacctcgtc gacataatct ggatattcct gttcccgctg
       61 ttctacctca tcagctaggc ggaacggcga ccgatacccg tggaaaggag ggcatgctaa
      121 aggtgagcga aaccagggaa cccgcaaagc cgacgacgaa gtcctacctc gtgatctggg
      181 cggccctcgt gctgctgacc gggctcacgg tgaccatggc cagcctgaac ctcgggaggg
      241 ccgcgatctt ctcggtcctc gccatcgcgg cgctcaagtc cagtcttgtg ctcttctact
      301 tcatgcacct gcgctacgag ggaaggctcc tcataaggct cctcgtgccc atcgtgctcg
      361 ccaccctggc gatcttcatc gggctcacct acacggacat cctgtaccgc taacggcagg
      421 gccgcgctcc cggcgaaggg cgcgctggcc gaggaggaag gcatgacaag cacggcatcg
      481 gtgaacgagg ccttctattt catcacggcc gtctcggccc tcctgttctt cggcatcgtg
      541 ttcctcatga tcctcttcct cgtgcgctac cgccgctcgc gcaaccccga ggccaccgag
      601 atcccgggca atctctggct cgaggtcacc tggatcgtgg tgcccaccgt catcgtcctc
      661 gtgatgttcg tgtacgggct cacgggcttc accctgcagc gcaccaaggt ggcgggctcc
      721 ctcgaggtcg aggtgcacac gaggcagtgg tccttcctct tcgagtatcc gagcggcatc
      781 aagagccccg acctcgtggc gccctcgggc cgggacgtca ggatcaccct cgagtctgac
      841 gacgtcatcc acgccttcta cctgcccgcc taccgcgtcc agatcgacgc cgtcccgggc
      901 atgaagacct acgccaactt ccgcacgggg gccgtgggct ccttcgacat cctctgcgcc
      961 cagtactgcg gcaccctcca ctcctcgatg ctcgccaagc tctacgtcct gccccccgcc
     1021 gactacgacc gctggaccag gggcgagaag gtccagctcg cgggcaagaa cctgccgccc
     1081 accgcggcga cggggctcga gctgctgcgc cagaggtcct gcctcgcctg ccactcaacg
     1141 gacggcagcc ccctcgcggg cccgaccttc aagggcctct tcggctcgac cgtcgaggtg
     1201 atgacggcgg ggtccaagcg gagcgtcgtg gccgacgagg ccttcatccg caagtcgatc
     1261 gtcgacccgg ccgccgacat cgccgtgggc tttcagaaca tcatgccctc gggcaaggac
     1321 agctttggca aggacgacct cgacgaggcc gtcgaggcga tcaaggccct gaagtagggg
     1381 ccctgcccga cttcagaccg cgccggaccc tcgccgcgcc ggccctcgga cctccttcag
     1441 cctggccctc cttcagcctg gccccccctc agccgcgcca ggccttgccg cgcaggtcct
     1501 cccaggtgcc gccgttcgtg aagagctcgg ggtggagggt ccgcaggcgg gggtcctcgg
     1561 ccacgtcgtg ctggagcacc atccacttgg ggtaggggat gccggggttg gaggcgtgct
     1621 cgagctcgcg tctgtcctca tcgctcaggt ccagatcgct cgccgcgagg ttgtcctcga
     1681 ggtgctcgag gctcctggcc gccgcgatga cgctcgtgat ggccggcctc cccaggagcc
     1741 aggccagggc cacgcgggcc gggctgaccc cgagcctcgc cgcgaccttc ctctgggcct
     1801 cgaccaccct ccatccgtag tccgcgtcga agggcacgaa gcgacccgcc tcggcgaagc
     1861 gcgtgccctc gggtgggggg gaggagccct ggtacttccc cgagaggaag ccgccggcga
     1921 gggggctcca gacgaggatc ccgaggccct ggtcgagcgt gagcgggacg agctcgtact
     1981 cggcgtcgcg ggcctgcagc gagtagtaga tctgc
//
LOCUS       DASSQV010000153         5200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_784800, whole genome shotgun sequence.
ACCESSION   DASSQV010000153 DASSQV010000000
VERSION     DASSQV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784800"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /gene="proC"
                     /locus_tag="VFL04_02680"
     CDS             <1..886
                     /gene="proC"
                     /locus_tag="VFL04_02680"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET7838639.1"
                     /translation="TPTSTASRPKGTRTAPEPSQMRTTGYRLGIIGAGKIGEAIAAGV
                     VGRGLVAPAEIVLSVRTEKHRAELEARTGLRCLCDNLELARLADTIILAVKPKSVAEV
                     LGEIGPELGPERLLISTAAGVSLELLESRVPKGVQVVRTMPNMAVAIGEGMTVLSPGS
                     SVSPERLEWASSIFRALGRALVLEEQHMDAVTGLSGSGPAYVYIIIESLADGGLKLGL
                     PREVATELAAQTVLGAARNVLVTGEHPAKLKDQVTTPAGVTIDGILELEAGRLRVTLI
                     NAVVKAAERARQLLHRPH"
     gene            943..2073
                     /gene="alr"
                     /locus_tag="VFL04_02685"
     CDS             943..2073
                     /gene="alr"
                     /locus_tag="VFL04_02685"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7838640.1"
                     /translation="MEYCPPPETDRPTRAEIRLGALRRNFRRAKALAGAGVKVMAVVK
                     ANAYGHGIVRVARELLAEGADHIGVAYLEEGVCLREAGIETPILVFGAPNHDQIPSFI
                     EYDLELSVPSAGMARAISAAARDAGTPAKVHLKVDTGMERIGEHWYSCEAFFDEVLDL
                     PGLEIHGVFSHFATSDDDLDFAREQLERFRSVLAILESRGRLPPLVHIANSAGLVNLP
                     ESRFTMVRPGILLYGYEPTPLRTIGFEPVMRLMSRVAFLKEVRSGAGVSYGRIWIAPA
                     ATRIATIPIGYGDGYSRSLSNRAEVHIGGARHPVVGRVCMDQIMVDLGSAQAREGDEV
                     VLFGERAGGGLPAELLCGLLGTIPYELTCMVSARVPRIYVEE"
     gene            2142..4352
                     /locus_tag="VFL04_02690"
     CDS             2142..4352
                     /locus_tag="VFL04_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7838641.1"
                     /translation="MFRNSSIRTKLLVPIFGLLALVFPFLIFIIYTQISGVTTDFAYK
                     QTANVSAELGSRIGAKLEEGIQSAHTAGAALAALKGRSRDGRDLADRTLVTILNSEPS
                     FSGIEAGFETGAFDGADAAYAERRPDSDRLGRFMARYFRDASGSPAIEATPDQDQVSW
                     YLKARGLKSPGVTEPFMLTTGAGSSFVASCFAPIMSGEELKGAVAVDFPLSSVQAIVA
                     EERPLGTGQAALYSNETLIVAHADKERIGRSIRETESELIGDHMEEVIRAVSEATPLS
                     FVSRSGKGGSPVLVSITPVRIGQSDKPWALAISVPLSSVLAKQSGILAFMAAIGAGTL
                     ILVFAIVFFLAAAVVRPIRRTADLLEDIAMGEGDLSHRMPVGGTDEVSRMARNFNAFI
                     EKLQQIVVSIRTAGGGLEETGGELRQSMEETAAAVAQIAASARSVNERVLSQASSVSE
                     STGEVERISSTIGALETRIEEQAGGIAESSSAVEEMVANIASVARTVGRLGDSFETLL
                     SASESGRGALSALNVRIREIATQSENLLETNQVIAGIASQTNLLAMNAAIEAAHAGEA
                     GRGFSVVADEIRKLAEMSGARAKATANELRSIKATIDAMVGTSTGAEQEFSTILELIS
                     SLDGLRREIESAMEEQGTGSSRVLESLERMNASTREIREGSGEMTAGGKAVLREMESL
                     LALTEEIRAGMGEIARGAGEIDEAVRRVAGLAERNGEHIASVIREAGKFKVETA"
     gene            complement(4349..4966)
                     /locus_tag="VFL04_02695"
     CDS             complement(4349..4966)
                     /locus_tag="VFL04_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7838642.1"
                     /translation="MTEGLKVGRGKKIVDTSVVVEAAIALIETKGFDEFSTRRLAAEL
                     RISAMTLYNYYDNRDAILKETVLRGFQIFWEGLPEKIDVCYNACANPLRVYAVLAEHL
                     IEFAVARPNLYRFLFWADLGALMRDQTILLRYREAFTGVIERVEDRTAIEAIGDDVYL
                     FEVLINALAMNVLAGRSGMSPERYRELASEGYDRLLGRHEAAMRA"
     gene            complement(5017..>5200)
                     /locus_tag="VFL04_02700"
     CDS             complement(5017..>5200)
                     /locus_tag="VFL04_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838643.1"
                     /translation="IHARLMSLVRAAPDAKFEEPTPRWEVPIGKAVAWVFGHDSYHAS
                     QLRNMGVPGLKEPKEV"
BASE COUNT          873 a         1781 c         1757 g          789 t
ORIGIN      
        1 gacgcctacc tcgacagcct cgagaccgaa ggggacgcgc acggcgcctg aacccagcca
       61 gatgcggaca accggctaca gactcggcat catcggcgca gggaagatcg gcgaggccat
      121 cgccgcgggc gtggtcggcc gcggcctcgt cgccccggcc gagatcgtcc tttcggtccg
      181 cacggagaaa caccgagccg agctcgaggc gaggacgggg ctccgctgtc tctgcgacaa
      241 cctcgagctc gcccgcctag ccgacaccat catcctcgcc gtgaagccca agtccgtcgc
      301 cgaggtcctc ggggagatcg gccccgagct ggggccggag aggctcctca tctccacggc
      361 cgccggggtc agcctagagc tcctcgagtc cagggtcccc aagggcgtcc aggtcgtcag
      421 gaccatgccg aacatggccg tcgccatcgg tgaggggatg acggtcctgt cgccgggcag
      481 ctcggtcagc ccagagcggc tcgagtgggc gagctcgatc ttcagggccc tcggcagggc
      541 cctggtgctc gaggagcagc acatggacgc ggtcaccggg ctctccggat cgggcccggc
      601 ctacgtctac atcatcatcg agtccctcgc cgacgggggc ctcaagctcg gcctgccccg
      661 cgaggtcgcc accgagctcg cggcccagac cgtgctcgga gcggcgcgca acgtgctggt
      721 gacgggcgag caccccgcga agctcaagga ccaggtgacc acccccgcag gagtgaccat
      781 cgacggcata ctcgagctcg aggcggggcg gctgcgcgtg accctcatca acgccgtcgt
      841 gaaggccgcg gagcgggcgc gccagctcct ccaccgcccc cactagcccc ccccggcttc
      901 cgattgcccc ggcggatggg gcggtgtatc attcccctcc agatggagta ctgccctccc
      961 cccgaaaccg accgtccgac ccgggccgag atacgcctcg gggccctccg caggaatttc
     1021 cggcgcgcca aggccctcgc cggagccggc gtgaaggtca tggcggtggt caaggcgaac
     1081 gcctacggac acggcatcgt cagggtcgcc cgcgagctcc tcgccgaggg cgccgaccac
     1141 ataggcgtcg cctacctcga ggagggggtc tgcctgaggg aggcggggat cgagacgccg
     1201 atcctcgtgt tcggcgcccc gaaccacgac cagatcccga gcttcatcga gtacgacctc
     1261 gagctcagcg tcccctcggc gggcatggcg agggcgatct cggcggcggc ccgggacgcg
     1321 gggacccccg cgaaggtcca cctgaaggtg gacaccggga tggagaggat cggggagcac
     1381 tggtactcct gcgaggcctt cttcgacgag gtgctcgacc tccccggact cgagatccac
     1441 ggggtcttct cgcatttcgc cacctcggac gacgacctcg atttcgcgag ggagcagctc
     1501 gagcgcttcc gctcagtcct cgccatcctc gaatcgaggg ggaggctccc tcccctcgtg
     1561 cacatcgcca attcggctgg actcgtgaat ctgcccgagt cgcgcttcac catggtgagg
     1621 ccggggatcc tcctctacgg ctacgagccc acgcccctca ggacaatcgg cttcgagccc
     1681 gtcatgcggc tgatgtcgag ggtggccttc ctcaaggagg tccgctccgg cgcgggcgtg
     1741 agctacggga ggatctggat cgcgccagcg gccacgcgga tagccacgat cccgatcggc
     1801 tatggggacg gctacagccg cagcctctcc aacagggccg aggtccacat cggcggggcg
     1861 aggcaccccg tggtgggcag ggtgtgcatg gaccagatca tggtcgacct cggctccgcg
     1921 caggccaggg agggcgacga agttgtcctg ttcggggaga gggcgggagg ggggctgccc
     1981 gccgagctcc tgtgcggcct cctcgggacc atcccttatg agctgacctg catggtgagc
     2041 gcgcgcgtgc cgcgtatcta cgtggaggaa tagcccggcc ggattgaaac aagccccaaa
     2101 atcgctataa tgcagggatt cctgccgatt tcggagtgcc catgttccgc aactcctcca
     2161 tcaggaccaa gctcctcgtg cccatattcg gccttctggc cctggtcttc cccttcctga
     2221 tcttcatcat ctacacgcag atctcggggg tcaccaccga tttcgcctac aagcagacgg
     2281 ccaacgtctc cgccgagctc ggcagcagga taggcgcgaa gcttgaggag gggatccaga
     2341 gcgcgcacac cgcgggggcg gccctcgccg cgttgaaggg caggtcgaga gacggccgcg
     2401 acctcgccga caggaccctc gtcaccatcc tgaactccga gccctcgttc tcggggatcg
     2461 aggcgggctt cgagaccggg gccttcgacg gcgccgacgc cgcctacgcg gagcggaggc
     2521 ccgactcgga ccggctcggg cgattcatgg cgcgttactt ccgcgacgcc tccgggagcc
     2581 ccgccatcga ggcgaccccc gaccaggacc aggtaagctg gtacctgaag gcgagggggc
     2641 tcaagagccc cggcgtgacc gagcccttca tgctcacgac cggagcgggc tcctccttcg
     2701 tggcctcctg tttcgccccg atcatgtcgg gggaggagct caagggggcc gtcgccgtcg
     2761 acttcccgct ttcctccgtc caggccatcg tcgccgagga gcgtcccctg gggaccggtc
     2821 aggcggccct gtactcgaac gagaccctca tcgtggccca cgccgacaag gagaggatag
     2881 ggcgctccat acgcgagacc gagtcggagc tcatcgggga ccacatggag gaggtcatcc
     2941 gcgccgtctc cgaggccacg cccctcagtt tcgtgtcgcg ctcgggcaag gggggctccc
     3001 ccgtcctcgt atcgataacg cccgtgagga tcgggcagtc ggacaagccc tgggccctcg
     3061 ccatctcggt ccccctctcg agcgtcctcg ccaagcagtc gggcatcctc gccttcatgg
     3121 cagcgatcgg ggcaggcacc ctgatcctcg tcttcgccat cgtcttcttc ctcgccgccg
     3181 cagtcgtcag gcccatacgc cgcaccgccg acctccttga ggacatagcc atgggcgagg
     3241 gcgacctttc ccaccggatg cccgtgggcg gcaccgacga ggtctccagg atggcccgca
     3301 acttcaacgc cttcatcgag aagctccagc agatcgtggt ctcgatcagg acggccggcg
     3361 gcggtctcga ggagactggc ggcgagctca ggcagagcat ggaggagacg gcggcggcgg
     3421 tcgctcagat cgcggcgagc gcgaggagcg tgaacgagcg ggtcctgagc caggcctcga
     3481 gcgtctccga gtccaccggg gaggtcgaga ggatctcctc gaccatcggg gccctcgaga
     3541 cccggatcga ggagcaggca ggggggatag cggaatcctc ctcggccgtc gaggagatgg
     3601 tggccaacat cgcctccgtg gccaggaccg tgggccggct cggcgacagc ttcgagaccc
     3661 tgctctcggc ctcggagtca ggcaggggag cgctgagcgc cctcaatgtc aggatacgcg
     3721 agatagccac ccagtcggag aacctgctcg agaccaacca ggtcatcgcc ggcatcgcct
     3781 cccagaccaa cctcctcgcc atgaacgccg ccatcgaggc agcccacgcc ggcgaggccg
     3841 gtcgcggctt ctccgtggtc gctgacgaga tcaggaagct ggccgagatg tccggggcga
     3901 gggccaaggc cacggccaac gagctccgct cgatcaaggc caccatcgac gcgatggtcg
     3961 gaacctcgac cggggccgag caggagttct cgaccatcct cgagctcatc agctcgctcg
     4021 acgggctccg gagggagatc gagagcgcga tggaggagca gggaacgggc tcatcccgcg
     4081 tgctcgaatc cctcgagcgc atgaacgcct cgacgaggga gatcagggaa gggtccggcg
     4141 agatgacggc cgggggcaag gccgtcctca gggagatgga gagcctcctc gccctcaccg
     4201 aggagatcag ggcggggatg ggcgagatcg ccaggggcgc tggggagata gacgaggccg
     4261 tccgccgcgt cgccggcctc gccgaacgga acggtgagca catcgcctcg gtgatcaggg
     4321 aggcgggcaa gttcaaggtc gagacggcct aggccctcat cgcggcctcg tgccggccga
     4381 ggagcctgtc gtagccctcc gaggctaatt cccggtagcg ctccggcgac atgcctgagc
     4441 ggccggcgag cacgttcatc gcgagggcgt tgatcaggac ctcgaagagg tagacgtcgt
     4501 cgccgatggc ctcgatggcc gtcctgtcct cgaccctctc gatgactccc gtgaaagcct
     4561 cgcggtagcg caagagtatg gtctggtcgc gcatgagggc gccgaggtcg gcccagaaca
     4621 ggaagcggta gaggttgggc ctcgcgaccg cgaactcgat gaggtgctcg gcgaggacgg
     4681 catagacccg cagggggttg gcgcaggcgt tgtagcagac gtcgatcttc tccggcaggc
     4741 cctcccagaa gatctggaag ccgcggagga cggtctcctt gaggatcgcg tcccggttgt
     4801 cgtagtagtt gtagagcgtc atcgcggaga tccgcagctc ggccgcgagg cgccgagtcg
     4861 agaactcgtc gaagcccttc gtctcgatca gggcgatcgc tgcctccacg accacgctgg
     4921 tgtcgacgat cttcttgccc ctgcccacct taaggccctc cgtcatgaat catcatgctt
     4981 cgcggacggc atttcaatat ccatcctgac gggcgctcag acctccttgg gctccttgag
     5041 tcccggcacg cccatgttcc gcagctggga cgcgtggtag ctgtcgtggc cgaagaccca
     5101 ggccacggcc ttcccgatcg gcacctccca gcggggcgtc ggctcctcga acttggcgtc
     5161 cggagcggcc ctcacgagac tcatgaggcg ggcgtggatc
//
LOCUS       DASSQV010000154         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1779808, whole genome shotgun sequence.
ACCESSION   DASSQV010000154 DASSQV010000000
VERSION     DASSQV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779808"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..819
                     /locus_tag="VFL04_02705"
     CDS             166..819
                     /locus_tag="VFL04_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838644.1"
                     /translation="MYAFLVLGLPLGFLLLYIGIYPREEAIPTRRAYLRGLVASIPVY
                     LVARLLGAIVPSVYGSPLFALHEWAARFLPFAGLPVLLYLAFYHFGERLGPGSLERRL
                     TAFHAGALSPIGLYEMVRIWGSPNPYDVLIFPFLLAATALALPRIAISVDGSYGFGLV
                     LRVAGAALASLAISICPTLILARLWPLIWLVAAAAGAGAWFFARGGLEARAPVSLSE"
     gene            complement(902..1900)
                     /locus_tag="VFL04_02710"
     CDS             complement(902..1900)
                     /locus_tag="VFL04_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET7838645.1"
                     /translation="MTSYKELGLVNTVDLFKRAVKGGYALPAYNFNNLEQLQAIIMAC
                     VETRSPVILQVSSGARKYANQNLLRNMAKGAVEYAHELGYDIPIVLHLDHGDSFELCQ
                     NCIESGFSSVMIDGSHLAYAENVALTKKVCDYAHSRKDYVSVEGELGVLAGVEDEVSA
                     EHSNYTKPEEVQDFVAKTGVDSLAISIGTSHGRTKFKREQCTETADGILIPPPLRFDI
                     LEAVEKMLPGFPIVLHGSSSVPIEYVKMVEKYGGKLSDSVGIPEEQLRRAAKSAVCKI
                     NIDSDGRLAMTAAIRKVLAEKPDEFDPRKYLGPARDELKKLYAHKNQNVLGSAGRA"
BASE COUNT          283 a          763 c          718 g          419 t
ORIGIN      
        1 gaagcgcgca tcgtgggccc aggaccacct cgaccttcag cagatctgcg ccgattgaag
       61 cccggcgagc ccgcctcgcc gctcgcccgc gagcccgcct cgtcgctcgt cctgtggggc
      121 ggcagtcatt gatcgcggct cgggggccgt gatagcctcc aaaccatgta cgccttcctc
      181 gtcctcggcc tgcccctcgg tttcctcctc ctgtacatag ggatctatcc gcgggaggag
      241 gcgatcccga cccgacgcgc ctatctgcgc gggctcgtgg catcgattcc ggtctatctt
      301 gtcgcgaggc tcctgggcgc catcgtcccc tccgtctacg gctcccccct cttcgccctc
      361 catgaatggg cggcgcgttt cctccccttc gccggcctgc ccgtcctcct ctacctcgcc
      421 ttctaccact tcggcgagcg cctcggcccg ggctctctgg agcggaggct cacggccttc
      481 cacgcggggg ccctctcccc catcgggctc tacgagatgg tcaggatctg gggcagcccc
      541 aacccctacg acgtgctcat cttccccttc ctcctcgccg ccacagccct ggcccttccg
      601 aggatcgcca tctccgtcga cggatcctac ggcttcggcc tcgtcctcag ggtcgccggc
      661 gcagccctcg ccagcctggc gatatccatc tgccccaccc tcattctcgc gcggctgtgg
      721 cccctcatct ggctggtcgc tgctgctgcg ggggccggcg cctggttctt cgcccgcggg
      781 ggcctcgagg ccagggcccc ggtctccctc tccgaatagc cgaggcccgc caacaggctt
      841 cgaggagcaa aaaaaggagg ccgccccggg ggcggcctcc ctctcttcgt gccgggggcc
      901 ctcaggccct gccggcgctt ccgaggacgt tctggttctt gtgggcgtag agcttcttga
      961 gctcatcgcg ggccgggccg aggtacttgc ggggatcaaa ctcgtcgggc ttctcggcga
     1021 ggaccttgcg gatcgcggcc gtcatggcga ggcgtccgtc cgagtcgatg ttgatcttgc
     1081 agacggcgct cttggcggcc cgcctgagct gctcctcggg gatgccgacc gagtcgctga
     1141 gcttgccgcc gtacttctcg accatcttca cgtactcgat ggggacgctc gaggagccgt
     1201 ggagcacgat ggggaagccg gggagcatct tctcgacggc ctcgaggatg tcgaagcgca
     1261 ggggcggcgg gatgaggatc ccgtcggcgg tctcggtgca ctgctcgcgc ttgaacttgg
     1321 tgcggccgtg gctcgtcccg atggagatgg cgagggagtc gaccccggtc ttggccacga
     1381 agtcctggac ctcctcgggc ttcgtgtagt tggagtgctc ggccgagacc tcgtcctcga
     1441 ccccggcgag gacgccgagc tcgccctcga cggagacgta gtccttcctc gaatgggcgt
     1501 agtcgcagac cttcttggtc agggcgacgt tctcggcgta ggcgaggtgg gagccgtcga
     1561 tcatgaccga ggagaagccg gactcgatgc agttctggca gagctcgaaa ctgtcgccgt
     1621 ggtcgaggtg gaggacgatg gggatgtcgt agccgagctc gtgcgcgtac tcgacggcgc
     1681 ccttcgccat gttccgcagg aggttctggt tcgcgtactt cctggcgccc gaggagacct
     1741 ggaggatgac cgggctccgg gtctcgacgc aggccatgat gatggcctgg agctgctcaa
     1801 ggttgttgaa gttgtaggcc gggagggcat agccgccctt caccgccctc ttgaagaggt
     1861 cgaccgtgtt gacgaggccg agctccttat agcttgtcat tcctgtgctc cttcggggaa
     1921 gaacatgatt tcgggtcata ctaaggaacc cctcggcgca gcgtcaagcc cgggccttcg
     1981 cgaggcgccc cggcttggcg cgccgtcccc gggccccgct gcgccgatgg acgaggccgg
     2041 ccctgagcgc gtaccatctg gccatgccaa ggacagcgcg gcggcggccg cccaccctgc
     2101 cactcatgat cctggccggt ctggccctat cgtgctgcgc ccggggaggg cccgtcctcg
     2161 tcgcggtgga tgaggccttc gcc
//
LOCUS       DASSQV010000155         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1282426, whole genome shotgun sequence.
ACCESSION   DASSQV010000155 DASSQV010000000
VERSION     DASSQV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282426"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFL04_02715"
     CDS             complement(<1..360)
                     /locus_tag="VFL04_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838646.1"
                     /translation="MRVRLLIALVLAAIACGLWAEPLPLEPIALPTGAFRSLLYPGLE
                     LGSHVDRVGPTIRSLCIDDRGPVELGLGAFVSEKLAARELGIPGPGSTFSFGLDGLVG
                     FGTAGDAMPPAPWTDFPL"
     gene            539..1168
                     /locus_tag="VFL04_02720"
     CDS             539..1168
                     /locus_tag="VFL04_02720"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET7838647.1"
                     /translation="MKLIFLGPPGAGKGTIADLAMEELRLPHISTGDLFRAAVKNGSP
                     LGLKVKGIMESGGLVPDDLTIALVTERLGEPDAAKGWILDGFPRTIPQAEALEKLAAA
                     DKVVNFEVADEIVVGRLSGRRVCRSCGKIYHEKNMPPKKAGVCDLDGGELYTRDDDKE
                     AAIKVRLETYRKQTAPLIDWYGKKGKLLTIDGVGAPAAVLVRFKDALKK"
     gene            complement(1232..2050)
                     /locus_tag="VFL04_02725"
     CDS             complement(1232..2050)
                     /locus_tag="VFL04_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7838648.1"
                     /translation="MSYIEEQFGLEEAVVVITGAGGILASSLAEAFVRAGAKVSLWVR
                     RSEVVEETRALYAPMASSPDRLQVLAADAGDKARVAAAYAETKAALGSPNVLVNAVGG
                     NKGKGPFVDADPSVFDDILRLNLMAGLLVPTQVFAAEWLKSGVKGSIINLASMGSYVP
                     LSGVWAYNAAKAGVLNLTQACAKEFGAAGIRVNAIAPGFFLGKQNKALLVANEATGEL
                     TPRGADVVRRTPFARFGKAEELRGAALFLANPKVSGFVTGVCIPVDGGFLVDCI"
     gene            complement(2109..2900)
                     /locus_tag="VFL04_02730"
     CDS             complement(2109..2900)
                     /locus_tag="VFL04_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET7838649.1"
                     /translation="MKAEPKEAGLYNPRLESILSLISRKRTVSVTELRERLGVSGVTV
                     RKYLDILEGEGIIVRTHGGAMMAEDRSSIRTISMREETRVEEKREIARAAKALVSEGD
                     VIFLDSGTSCRALARELSGMSLQVITTSLDVMEELAPSPSISLFCPGGSLRREARAFI
                     GPTAVEALRDLRIGTCFLGASGFSAEASFYTQNLVESQLKRQVLGVSRRRVVLADSAK
                     LGLEAFSVFARPGSLDLLVTDSGFPLAEEFRALGIEVITARSGQD"
     gene            3058..4287
                     /locus_tag="VFL04_02735"
     CDS             3058..4287
                     /locus_tag="VFL04_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HET7838650.1"
                     /translation="MNLTEVELRQQLFPVLDGLGRRRRVLALPPDLTRAHSRAGLVLK
                     LLYEYYGDALTDVLPALGTHEAMSEAEIECMYPGVPASLFRVHDWRKDLVTLGRVPSD
                     YVRELSEGKLSYDWPAQLNRLAAQGGHDLIVSIGQVVPHEVAGMANHAKNLFVGAGGV
                     EGIHRSHYLGAVYGMERIMGRVDTPVRALFDYASREFASSLPVLYALTVVGEGRLQGF
                     FVGQGRKCFEEACELSRSLNVKLLDRPIRKAVVSLEPGEFKSTWLGNKSVYRTRMAMA
                     DGGELVVLGPGVSRFGEDPTIDRLIRKHGYRGTEASLAAVERDPELAANLSAAAHLIH
                     GSGEGRFSITYCPGALSREEIEGVGYLWRDLKEAQAEYRPERLEPGFNTLPGGEEVFY
                     VPNPAAGLWATRERWGA"
BASE COUNT          711 a         1490 c         1534 g          625 t
ORIGIN      
        1 gagggggaaa tcggtccagg gcgcgggggg catggcgtcc ccggccgtcc cgaaaccgac
       61 gaggccatcg aggccgaagg agaaggtcga gcccggaccg gggatgccga gctcgcgcgc
      121 ggcgagcttt tcggagacga aggcgccgag gcccagctcg acggggcccc gatcgtcgat
      181 gcagagggac ctgatggtgg ggccgacccg gtcgacgtgg ctgccgagct cgaggcctgg
      241 gtaaaggagg ctgcggaagg cccccgtggg gagggctatc ggctcgaggg ggagtggctc
      301 ggcccagagc ccacaggcga tggcggcgag gaccagggcg atgagaaggc ggacgcgcat
      361 cgcccccacg atactgggcc ccaccgcccc ggcgtcaagc ctgggcgccg cggccccgcg
      421 ccgaccgcgg gcccgcgccg accgccgcct ggcgccggag gccggtgcct tgcggcgctc
      481 gggggctccg gattatcctt ggcccagatc cacatacgat agacaggaga acagggacat
      541 gaagctgata ttcctcggac caccgggcgc tggcaagggc accatcgccg acctcgcgat
      601 ggaggagctc cgcctccccc acatctccac cggcgacctg ttcagggccg ccgtgaagaa
      661 cggcagcccc ctgggcctca aggtcaaggg catcatggaa tcggggggcc tcgtccccga
      721 cgacctcacg atcgccctcg tgaccgagcg gctcggcgag cccgacgccg ccaagggctg
      781 gatcctcgac ggcttcccga ggaccatccc ccaggccgag gccctcgaga agctggcagc
      841 ggccgacaag gtggtgaact tcgaggtcgc cgacgagatc gtcgtgggca ggctctcggg
      901 ccgccgcgtc tgcaggagct gcggcaagat ctaccacgag aagaacatgc cgccgaagaa
      961 ggcgggggtc tgcgacctcg acggcgggga gctctacacc cgcgacgacg acaaggaggc
     1021 cgcgatcaag gtcaggctcg agacctaccg caaacagacg gcccccctca tcgactggta
     1081 cggcaagaag ggcaagctcc tcacgatcga cggggtcggc gccccggccg cagtcctcgt
     1141 gcgcttcaag gatgcgctaa agaaataggc ctcagcagaa gaccgccgca gaggcgcgga
     1201 gatgcctctg cggtgctggg tcttcccgct atcagatgca gtccaccagg aagcccccgt
     1261 ccaccgggat gcagaccccg gtcacgaagc ccgagacctt ggggttggcg aggaagaggg
     1321 cggccccacg gagctcctcg gccttgccga aacgggcgaa gggtgtgcgg cgcacgacgt
     1381 cggcgccccg gggggtgagc tcgcccgtgg cctcgttggc cacgaggagg gccttgttct
     1441 gcttgccgag gaagaagccg ggggctatcg cgttcaccct gatgccggcc gcaccgaact
     1501 ccttggcgca ggcctgggtg aggttgagga ccccggcctt ggccgcgttg taggcccaga
     1561 cccctgacag gggcacgtag gagcccatgg acgcgaggtt gatgatcgag cccttcacgc
     1621 ccgacttcag ccactctgcc gcgaagacct gggtgggcac caggaggccg gccatgaggt
     1681 tcagcctcag gatgtcgtcg aagaccgagg ggtcggcatc gacaaagggg cccttgccct
     1741 tgttcccccc gacggcgttg acgagaacgt tgggcgaacc caaggccgcc ttggtctcgg
     1801 cgtaggccgc cgcgaccctg gccttgtcgc cggcgtcggc ggcgaggacc tggaggcggt
     1861 cgggagagga ggccatgggg gcgtagaggg ccctcgtctc ctcgacgacc tcgctccttc
     1921 gcacccacag cgacaccttg gcccctgccc tgacgaaggc ctcggcgagg gagctcgcga
     1981 ggatgccccc cgccccggtg atgacgacga cggcctcttc gagcccgaac tgctcctcga
     2041 tgtagctcat tgtgttcctc cgcttcgata tgtggtgcgg gcccggccgg ctggccttgg
     2101 ccgcgctctc aatcctgccc gctccgagcc gtgatgacct cgatcccgag ggccctgaac
     2161 tcctcggcga ggggaaagcc cgagtccgtg acgaggaggt cgaggctccc cggccgcgcg
     2221 aataccgaga aggcctcgag cccgagcttg gccgagtcgg cgaggactac ccgccgcctc
     2281 gacactccga ggacctggcg cttgagctgg gactcgacga ggttctgggt gtagaagctg
     2341 gcctcggccg agaagcccga ggcgccgagg aagcaggtgc cgatgcgcag gtcgcgcagg
     2401 gcctcgacgg ccgtggggcc gatgaaggcg cgggcctccc ggcgcaggct ccctccgggg
     2461 cagaagagcg agatcgaggg cgagggcgcg agctcctcca tcacgtcgag ggaggtggtg
     2521 atgacctgga ggctcatgcc cgagagctcg cgggccaggg ccctgcagct cgtccccgag
     2581 tcgaggaaga tcacgtcccc ctcggagacc agggccttgg cggcccgggc gatctcgcgc
     2641 ttctcctcga ccctcgtctc ctcgcgcatc gagatggtgc ggatcgagga gcggtcctcg
     2701 gccatcatgg ccccgccgtg ggtcctcacg atgatcccct cgccctcgag gatgtccagg
     2761 tacttcctga ccgtcacccc ggacacgccg agccgctccc gaagctcggt gaccgacacg
     2821 gtccgcttgc gggagatgag gctgaggatc gactcgagcc ggggattgta gagcccggct
     2881 tccttgggtt ctgctttcat atggtttcga ttttcgtacg gtaagggaag ctctggccct
     2941 tgtcaagcct aagccgccct agggcgccct tggctgcatt gacacgccgg ggccgggcgg
     3001 gcgagtattc ccgggacagc caaaaagcga aagcaaacgc aagtatcggg agggcccatg
     3061 aacttgaccg aggtggagct cagacagcag cttttccccg tcctggacgg cctggggcgg
     3121 cgccgccgcg tcctcgccct cccgcccgac ctcacccgcg cccattcccg ggccggcctc
     3181 gtcctcaagc tgctctacga gtactacggg gacgcgctca ccgacgtcct gccggccctc
     3241 ggcacccacg aggcgatgag cgaagccgag atagagtgca tgtaccccgg agtccccgcc
     3301 tcgcttttcc gcgtccacga ctggaggaag gacctcgtga ccctaggccg agtgccctcg
     3361 gactatgtgc gcgagctctc ggagggcaag ctctcctacg actggcccgc ccagctcaac
     3421 cgcctcgccg cccagggcgg ccacgacctc atcgtctcca tcggccaggt cgtgccccac
     3481 gaggtcgcgg gcatggccaa ccacgcgaag aacctcttcg tcggcgcggg cggggtcgag
     3541 ggcatccaca ggagccacta cctgggcgcc gtctacggca tggagaggat catgggcagg
     3601 gtcgacacgc cggtgcgggc cctcttcgac tacgcgagcc gcgagttcgc ctcgtccctg
     3661 cccgtcctct acgccctcac cgtcgtgggg gagggcaggc tccagggctt cttcgtgggc
     3721 cagggcagga aatgcttcga ggaggcctgc gagctgtctc gatcgctcaa cgttaagctc
     3781 ctcgaccggc ccatacggaa ggccgtcgtg agcctcgagc cgggggagtt caagtcgacc
     3841 tggctcggca acaagagcgt ctaccgcacc cgcatggcca tggccgacgg gggcgagctc
     3901 gtggtcctgg gccccggggt ctcccgcttc ggcgaggacc ctaccatcga ccgcctgatc
     3961 cggaagcacg gctaccgcgg caccgaggcc agcctggcgg cggtggagcg cgaccccgag
     4021 ctcgcagcga acctctcggc cgccgcccac ctcatccacg gctcgggcga ggggcgcttc
     4081 tccatcacct actgcccggg ggccctgagc cgcgaggaga tcgagggggt cggctatctt
     4141 tggcgggacc tgaaggaggc ccaggccgag tacaggcccg aaaggctcga gccgggcttt
     4201 aacacccttc ccggcgggga ggaggtcttc tacgtgccca acccggcggc cgggctctgg
     4261 gccacgaggg agcgctgggg ggcttaggcc gagccggccc gccggcgctg gtggggcttg
     4321 tcggccttgg gcttgcgctg caggaggacc tcgacgtggg
//
LOCUS       DASSQV010000156         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1780432, whole genome shotgun sequence.
ACCESSION   DASSQV010000156 DASSQV010000000
VERSION     DASSQV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780432"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFL04_02740"
     CDS             <1..802
                     /locus_tag="VFL04_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7838651.1"
                     /translation="AKASGADPYSAWYLFTAAFAYERQGDAPIAAIYYDRIVKNWPDL
                     IVDGRSIHFECLSRLIETVSAPERRIEYYKDLLARFPERVDMGRILFLLAKEYEKVGD
                     WDLAIKTYARFLPYFGASIQGYPDAFQYARNVIDFYNSPKDWAYVDLQTLVSKIKAAL
                     AANDAYKLRGLRAKVNFFAVDWNKDEIDANSQALFDFSEFMSGGRISSADALDPGSGP
                     REAYLKTWGWSDRISTWYLYFRKIYFPADPEIHGRWEWAGIYFGERMR"
     gene            802..2193
                     /locus_tag="VFL04_02745"
     CDS             802..2193
                     /locus_tag="VFL04_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7838652.1"
                     /translation="MSANFRRAGAIALATVWAALAAAQGSAASMPFQGEATAAVPKPD
                     PRPIRTAEPAEATPDPGWGAAGPPCAGRAAAASYGLAMPWGQEDFEAFRAAYLSPDGR
                     KWLEAVMQRARPYLGYIEERLRLYALPEELAFLPVVESEFSPKAVSRSGAAGLWQFMR
                     NSISGYGMRIDDWVDERRDFMKSSDGALRKLADNYSTFGDWNIAIAAYNAGAGALGRA
                     IVAARRDGSDSPDYWELRRRGLLSRETSAYVPKFLAIASILMHAGRSGLATSWDSPKS
                     WEAFPPGKPIDLSLLASAAGLDLGLLRAANPELRYGVTPPTPGYLLKVPSEALPVVKA
                     VLEDPGRALIRYYLHTVRSGDTLSAISRRFGTPISAILQANPGLRPDLIRLGLSIVVP
                     AFKDGPAPEEAAPSKGENLDFSGSYTVAKGDSLWALSLRFNVQPEALAERNGLSITSV
                     IREGMTLRVPILN"
     gene            2198..>2850
                     /locus_tag="VFL04_02750"
     CDS             2198..>2850
                     /locus_tag="VFL04_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838653.1"
                     /translation="MSTRHAYRAARCAMALALSLMASGLGAQASPYSVDARVSWETRL
                     LQVKVRLDMQAAGIRLPAGRLEAERMIERDLPGLAKDPVFALRVDSRRSVADAVEDGS
                     LEVTELLRIADAARRTEASFTKDMKAFSATYEIPLDAIGAMFVQHSTPAAFPAASAWA
                     PSRPFTGIVIYAKGELPVHGEARSAQLAPCLFPRIFDESMELVLGPALVDPEVLRSRG
                     "
BASE COUNT          461 a         1031 c          910 g          448 t
ORIGIN      
        1 ggcgaaggcc agcggggcag atccctattc ggcctggtac ctcttcaccg cggccttcgc
       61 ctacgagcgc cagggggacg cccccatcgc ggcgatctac tacgatagga tcgtcaagaa
      121 ctggccagac ctcatcgtgg acggcaggtc gatccacttc gagtgcctgt cccggctcat
      181 cgagactgtc tcggccccgg agcgccggat cgaatactac aaggacctcc tcgcgcgttt
      241 ccccgaacgc gtcgacatgg ggcggatcct cttcctcctc gccaaggagt acgagaaggt
      301 cggggactgg gacctcgcca tcaagaccta cgcgaggttc ctgccctact tcggcgcgtc
      361 gatccaggga tacccagacg ccttccagta cgcgcgcaac gtcatcgact tctacaacag
      421 ccccaaggac tgggcctacg tcgacctcca gaccctggtc tcgaagatca aggccgccct
      481 cgccgccaac gacgcctaca agctccgcgg ccttcgggcg aaggtcaact tcttcgccgt
      541 cgactggaac aaggacgaga tcgacgcgaa cagccaggcc ctgttcgact tcagcgagtt
      601 catgtcgggc gggaggatca gcagcgccga tgccctggac ccaggctcgg gcccccgcga
      661 ggcatacctg aagacctggg gctggtccga ccgcatctcg acatggtacc tgtacttcag
      721 gaagatctac ttccccgccg accccgagat ccacggacgc tgggaatggg ccggcatcta
      781 tttcggcgag aggatgcgct gatgtcagcc aacttccgca gggcgggcgc catcgccctg
      841 gccacggtct gggccgcctt ggccgcggcc cagggcagcg cggcctcgat gccctttcag
      901 ggcgaggcca cggcagccgt cccgaagccc gaccccaggc ccatcaggac cgcagagccg
      961 gccgaggcca cacccgaccc aggctgggga gcggctggcc cgccctgcgc ggggcgggcg
     1021 gcggcggcca gctatggcct ggccatgccc tggggccagg aggatttcga ggccttccgg
     1081 gccgcctatc tgagcccgga cggcaggaaa tggctcgagg cggtgatgca gcgcgcccgg
     1141 ccctatctcg gctacatcga ggagaggctg cggctctacg ccctgcccga ggagctggcc
     1201 ttccttcccg tcgtcgagtc ggagttctcg ccgaaggcgg tgtcgagaag cggcgccgcg
     1261 gggctctggc agttcatgag gaactcgatc tcgggatacg ggatgcggat cgatgactgg
     1321 gtggacgagc gccgggattt catgaagagc tcggacgggg ccctccgcaa gctcgccgac
     1381 aactactcca ccttcggcga ctggaacatc gccatagcgg cgtacaacgc gggggccggt
     1441 gcccttgggc gggcgatcgt cgcggccagg cgcgacggga gcgactcccc cgactattgg
     1501 gagctccgga ggcggggcct cctgtccagg gagacctcgg cctatgtccc caagttcctc
     1561 gccatagcct cgatcctcat gcacgcgggc cgctcgggcc tggcgacgag ctgggacagc
     1621 ccgaaatcgt gggaggcctt ccccccgggc aagcccatcg acctttccct cctcgcgagc
     1681 gccgcgggcc tcgacctggg cctcctccgc gcggcaaacc ccgagctccg ctatggggtg
     1741 acgccgccga cccccggcta tctgcttaag gtcccctccg aggccctgcc cgtcgtcaag
     1801 gccgtcctcg aggacccggg ccgggccctc atcaggtact acctgcacac ggtccgctcg
     1861 ggcgataccc tttcggccat ctcgaggcgt ttcggcaccc ccatctcggc catcctccag
     1921 gcaaaccccg gcctgaggcc cgacctcatc cgcctcggcc tgtccatcgt cgtgcccgcc
     1981 ttcaaggacg ggccagcccc cgaggaggcg gctccatcca agggcgagaa tctggatttc
     2041 tcgggctcct acacggtggc gaagggggac agcctctggg ccctttccct ccgtttcaac
     2101 gttcagcccg aggccctcgc cgagcggaac ggcctcagca taacaagcgt gatccgtgaa
     2161 ggcatgaccc tccgcgtgcc gatactcaat taaggaaatg agcaccagac acgcataccg
     2221 ggcggcgcgc tgcgccatgg ccctcgccct ctccctcatg gcctcgggtc tcggggccca
     2281 ggctagcccc tactcggtgg acgcgagggt ctcctgggag acccgcctcc tgcaggtcaa
     2341 ggtgaggctc gacatgcagg ccgcggggat ccgcctgcca gcgggcaggc tcgaggccga
     2401 gaggatgatc gagcgcgacc tccccggcct ggccaaggac cccgtcttcg ccctccgcgt
     2461 cgactcgcgg cggagcgtcg ccgacgccgt cgaggacggc agcctcgagg tcaccgagct
     2521 cctcaggatc gccgacgctg cccgcaggac agaggcttcc ttcaccaagg acatgaaggc
     2581 cttctcggcc acctatgaga tccccctcga cgcgataggg gcgatgttcg tgcagcactc
     2641 gacgccggcg gcctttccgg ccgcctcggc ctgggcccca tcgcggccct tcacgggcat
     2701 cgtgatctac gcgaaggggg agctgcccgt ccatggggag gcccgcagcg cccagctcgc
     2761 gccctgtctc ttcccgagga tcttcgatga atccatggag cttgtcctcg gtccggccct
     2821 tgtcgacccc gaggtcctgc gcagccgggg
//
LOCUS       DASSQV010000157         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_165307, whole genome shotgun sequence.
ACCESSION   DASSQV010000157 DASSQV010000000
VERSION     DASSQV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165307"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1388)
                     /locus_tag="VFL04_02755"
     CDS             complement(3..1388)
                     /locus_tag="VFL04_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET7838654.1"
                     /translation="MQQNTQARLDLARPAADGLFLPDLLRIGVPIALQNLVSASVNML
                     GTVMVGQLGSAALAAVGLGNQIYFLLLIFLFGVSTGGGVFTAQYWGKRDIAGIRRTLG
                     LSYLVGMAVALAFTIAALAFPAGLIGLYSRDPEVISIGAGYLRVVALSYPPMAMSFVL
                     GLAMRSVEKVRLPLVATTLSLALSLSLSWLLIFGGLGIPAMGVVGAAWATVAARVVEA
                     LILFIVSYARRYPTAARLRELVDWGGAWVARFWIIALPVMLNEVTWSLGITTYNAIFA
                     RAGTGAVAAYSVITTVGNLAMVVFFGTANAAAVMIGKKIGEGRLEAAFTYARRFAIMA
                     PLIGVAVGAVLVPLRLLLPLVFRLEEAVLAQASIMLLVLAATVPFKAFNLHLVVGICR
                     SGGDTRFGMLYDLGGVWGLGVPLAALGAFVWGLPAWGIFLMTSSDEVGKSLFGVWRLM
                     SRKWIHDVTVH"
     gene            1475..>2186
                     /locus_tag="VFL04_02760"
     CDS             1475..>2186
                     /locus_tag="VFL04_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET7838655.1"
                     /translation="MLADAHIHLHNLLSRDPLFPERLAGQAWTGCAASHDEAEYLETE
                     ALRGPSGSGGLVFVTSFGIHPQWLVWKNADFLARLAREGRIAAIGEAGFDFFGDRPER
                     VRNPENEAAQRAVFEYQLGLAEAHGLPMILHLRKAMDLAFEYGPRLRRLRGAVFHSYS
                     GTDRDAASLIARGIGAWFSFGAPILNGNKRSRAACAAVPLDRILAETDAPWQPPSGSD
                     FCRPEHLKDVIKGMAAIRG"
BASE COUNT          374 a          750 c          795 g          267 t
ORIGIN      
        1 acctagtgga cggtcacgtc gtggatccat ttcctcgaca tgaggcgcca gacgccgaag
       61 agggacttgc ccacctcgtc cgaggaggtc atcaggaaga tcccccaggc gggcaggccc
      121 cagacgaagg cgccgagggc ggcgaggggg acgccgaggc cccagacccc gccgaggtcg
      181 tagagcatgc cgaaccgggt gtccccgccc gaacggcaga tgccgacgac gaggtggagg
      241 ttgaaggcct tgaaggggac ggtggccgcg agcaccagga gcatgatcga ggcctgggcc
      301 aggacggcct cctcgagccg gaacacgagg ggcagcagga ggcgcagcgg gacgaggacg
      361 gcgcccaccg cgacgccgat gaggggggcc atgatggcga agcgccgggc gtaggtgaag
      421 gctgcctcga gccggccctc cccgatcttc ttcccgatca tgacggccgc ggcgttggca
      481 gtcccgaaga agaccaccat agcgaggttg cccaccgtgg tgatcacgct gtaggcagcg
      541 actgcccccg tgcccgcccg cgcgaagatc gcgttgtagg tggtgatgcc gaggctccag
      601 gtcacctcgt tgagcatgac gggcagggcg atgatccaga agcgggccac ccaggccccg
      661 ccccagtcga cgagctcgcg cagccgcgcc gccgtggggt agcgccgcgc gtaggagacg
      721 atgaatagga tgagggcctc gacgactcgc gcggcgacgg tggcccaggc ggctccgacg
      781 acccccatgg cggggatgcc gaggcccccg aagatgagga gccaggagag cgagaggctc
      841 agggcgaggg agagggtggt ggccaccagg gggagccgca ccttctcgac gctccgcatc
      901 gcgaggccga ggacgaagct catcgccatg gggggatagc tgagcgcgac gacgcggagg
      961 tagcccgcgc cgatcgagat gacctcgggg tcgcgcgagt acaggccgat gaggccggcg
     1021 gggaaggcca gggcggcgat ggtgaaggcc agggcgaccg ccatgcccac gaggtaggag
     1081 aggcccaggg tccgcctgat gcccgcgatg tcgcgcttgc cccagtactg ggcggtgaac
     1141 acccctccgc ccgtcgagac gccgaagagg aagatgagga ggaggaagta gatctggttg
     1201 ccgaggccca ccgcggcgag ggcagccgag ccgagctggc ccaccatgac ggtgccgagc
     1261 atgttgacgc tcgccgacac gaggttctgg agggcgatgg ggacgccgat cctgagcagg
     1321 tcgggaagga aaaggccgtc tgcggccggc ctggccaggt cgagccgggc ctgggtattc
     1381 tgctgcacga tgcgcgaggc tacgcgggat tcgggaggcc gtcaagcggc gccggcccgt
     1441 tcgcgggaca ggccccccgt gctacaatgg gccgatgctc gccgacgccc acatccacct
     1501 ccacaacctc ctctcccgcg atcccctctt ccccgagcgg ctcgcgggcc aggcctggac
     1561 cggctgcgcc gcgagccacg acgaggcaga gtacctcgag accgaggccc tccgcggccc
     1621 ctcgggatcc ggcgggctcg tcttcgtgac gagcttcggc atccaccccc agtggctggt
     1681 ctggaagaac gccgacttcc tcgcccgcct ggcccgcgag ggcaggatcg ccgccatcgg
     1741 cgaggcgggc ttcgacttct tcggcgaccg gcccgagcgc gtccgcaacc ccgagaacga
     1801 ggccgcgcaa cgcgccgtgt tcgagtacca gctcgggctc gcggaggccc acggcctgcc
     1861 catgatcctc cacctcagga aggcgatgga cctggccttc gagtacggcc cgaggctgag
     1921 gcgcctcagg ggggcggtct tccactccta ctcggggacc gaccgcgacg cggcctccct
     1981 catcgcccgg ggcatcggcg cctggttctc cttcggggcc ccgatcctca acggcaacaa
     2041 gcgctcgagg gcggcctgcg ccgccgtgcc cctggaccgc atcctcgccg agaccgacgc
     2101 gccctggcag cccccctcgg gctcggactt ctgccgtccc gagcacctca aggacgtcat
     2161 aaaaggcatg gccgccatcc gcggca
//
LOCUS       DASSQV010000158         6208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1530839, whole genome shotgun sequence.
ACCESSION   DASSQV010000158 DASSQV010000000
VERSION     DASSQV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530839"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFL04_02765"
     CDS             <1..214
                     /locus_tag="VFL04_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HET7838656.1"
                     /translation="YRVFLQEAEAVPMAKELKGTYLKAVFDRPLRDVVSLIIDNGIAH
                     HASAVYGSFVEPFRIAAKIAGWEVIE"
     gene            211..2202
                     /locus_tag="VFL04_02770"
     CDS             211..2202
                     /locus_tag="VFL04_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET7838657.1"
                     /translation="MIDIEAAQDGFVLGVDGRKVLAHTRRAPCVEIGVAETVLRQHRG
                     SVRLHRHRAAPIPLRSYRILESAPEFFAVDFDGRLRMAARLEAGRLRISFSLYDAAIN
                     FFRIRLAACHDEGIFGCGERFAHLDLKGRRLRLWVEGEREGSGRARADRDASSFPAPV
                     FVSTRSYWCAVDTQAHATIDFSRRTSTLLEAWAVPREIVMGFGADLSRHQAGSGSCLS
                     ATALVSDMTAFLGRQALPPSWVHDGAIIGVQGGIEELRAKAELCLRSGAKVAGFLIKD
                     WNGAKAGPKRSARDWTWDSALYPALPRELAALRAKGLRVLASVSPMLPVDSSQYAEAS
                     AAGHCVKAPDGRNYTMGAPGPVSGMLDLTSPLALAWAKELLKRELLGIGMSGWVAEPG
                     ECLPPDAVLSSGEDASLAHNRWPVLWARANREAIEEAGLLGEALFLSRTGWLGSTRYA
                     MAFWVGDGSDGRSKGAGFAGSIPAGVSAGLSGVGFWQFDVAGALSSARARGGSEGLVR
                     RMEMAAFTPFFVAEDEGGSGSWAGPESLAMLARMTEVFAALKPYHLAVAAEYRDSGLP
                     TIRHPWIHYEGDEAVQGLSRQYLYGRDLMVAPSLGGSEALTGLYLPEDGWVHLWSSRE
                     FRGGSVTVESPPGYPAVFYRSESRFASLFDAIRRTARRV"
     gene            2226..3035
                     /locus_tag="VFL04_02775"
     CDS             2226..3035
                     /locus_tag="VFL04_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838658.1"
                     /translation="MRSALLAAGAALLGNFLLSLGMTLQKRHIGWIGRKGPRDAAFRL
                     DLLGWLAGFALMNIQPVFNDLALLGLPPNVVGAAAGSSVAFTAILASVLLHEGLGLRR
                     LLWTGAMFAAMGLSAFHAEGSGTRVLPLALYVSLVPPLIAAALALLARRRRRGPGTAL
                     ALAAVAGSLGGFMVLPLAALRLVGGGDFSLWLGTPYLYLYIVAGVGAFSVVQLAYKDG
                     RMSQVAPAYYGMQVLWPALASYFAFAAPFDPVQAAAFVAVALCVVAIAAER"
     gene            complement(3042..4259)
                     /gene="ispF"
                     /locus_tag="VFL04_02780"
     CDS             complement(3042..4259)
                     /gene="ispF"
                     /locus_tag="VFL04_02780"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET7838659.1"
                     /translation="MPSPQPAGLAVLVTAAGRSERFGGGKKELVRIEGRSVLDRAVSP
                     FLSLDGLRALAITAPPGMEEEVRSSLSRDSLDALESRLGSSFIVTAGGGDRRDSVRMG
                     LEALALSPGLSGLALDEVVVLVHDGARPWASRDLAARVAQAALERGAAVPVVPLVDTP
                     KQIDAAGLVSGHPRRSDLAAAQTPQGFRFGGLLEAHRRAAAEGYACTDDTELWARYKG
                     AVASVPGEPGNRKLTRREDLPTPAALPFRIGQGWDLHRLVPNRRLLVGGVVLPFELGE
                     DGHSDGDVLFHAVIDALLGAAALGDIGSHFPPGDEAWRGADSSALAREAVSLVRASGW
                     EPGNLDCTVVLERPRLGPSRDEIRSSLARALGMELGSVSVKAKTKEGVDAAGEGRAIE
                     AYASVTLFRRPGF"
     gene            complement(4311..4811)
                     /locus_tag="VFL04_02785"
     CDS             complement(4311..4811)
                     /locus_tag="VFL04_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET7838660.1"
                     /translation="MDKGTPTDFAVDQRIVYPSQGVGRIVDIKPKNFKDSKLLYYIIY
                     LEFSDMTVMVPVDKALDLGIRAIVPKGEAEKALAFISEEYAPIPTDWKLRYQMNLDLL
                     KKGSVLDIASVVRSLYHRSKIKELPILERKLYDSALNLLQDEISFSLGKSKEEIAELI
                     RARLET"
     gene            4952..5623
                     /locus_tag="VFL04_02790"
     CDS             4952..5623
                     /locus_tag="VFL04_02790"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET7838661.1"
                     /translation="MLGFPLLIVALVASAIVFRHELLAIFRDRDALSAWIGARGAWGV
                     LAFVGLQVAQVVVFVIPGEIVQVAGGYVYGLWYGTLLSVLGITIGSLINFYLGRLLGR
                     PFVEALFDRERIESIERVTASGKAAAGFFLLFIIPGIPKDALCYIAGVSGLGFPAFFG
                     ISMFGRLPGILGSSFMGSAAHDRSFASAIVVLSVAAVLFCIGLFFKSRIESILVRLLH
                     GGRRK"
     gene            5655..>6208
                     /locus_tag="VFL04_02795"
     CDS             5655..>6208
                     /locus_tag="VFL04_02795"
                     /inference="COORDINATES: protein motif:HMM:NF044362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838662.1"
                     /translation="MNQGKRASPDARERLLSGLLRVCAGGGLLAYLPSAYLSVVQGYW
                     SVFVADTVACVYVILLALRPAIPARVKIASLLVISYLLGLMLLFTTGPFGAGHLFIFA
                     FVLLAALFGRLRGIIAANVLAIATHVGIAVASALKLVPWPQSLSSVVVISANFILVSL
                     VLSFSTNFLIRSLETSAGEERKLR"
BASE COUNT          808 a         2239 c         2108 g         1053 t
ORIGIN      
        1 ctaccgcgtc ttcctccagg aggccgaggc cgtgccgatg gcgaaggagc tcaaggggac
       61 ctacctgaag gccgtcttcg accgccccct ccgcgatgtg gtgtcgctca tcatcgacaa
      121 cggcattgcg caccacgcct cagcggtgta tggttccttc gtcgaaccgt tccgcattgc
      181 cgcgaagatc gctggctggg aggtcatcga gtgatagaca tcgaggccgc gcaggatggg
      241 tttgtcctgg gcgtcgacgg acggaaggtg ctcgcccaca cgcggcgcgc cccctgcgtc
      301 gagatcggcg tggccgagac cgtgctgcgg cagcaccgcg gctcggtcag gctccacagg
      361 caccgcgcgg ccccgatccc tctgcgctcc taccggatcc tggagagcgc tccggagttc
      421 ttcgccgtcg atttcgacgg aaggctcagg atggccgcca ggctcgaggc cggccggctg
      481 cggatctcct tctcgctcta cgacgccgcc atcaacttct tccgcatccg cctcgccgcc
      541 tgccacgacg agggcatctt cggctgcggc gagcgtttcg cccatctcga cctcaagggc
      601 cgcaggctga ggctctgggt cgagggcgag cgcgagggct ccggcagggc ccgcgccgac
      661 cgcgatgcga gctctttccc ggcccccgtc ttcgtgtcca cgaggagcta ctggtgcgct
      721 gtcgacaccc aggcccacgc cacgatcgac ttctcgcggc ggaccagtac cctcctcgag
      781 gcctgggccg tgccccgcga gatcgtgatg ggtttcggcg ccgacctttc tcggcatcag
      841 gcgggctcgg gcagctgcct ctccgccacc gcactggtgt ccgacatgac ggccttcctc
      901 gggcgccagg ccctgccccc gtcctgggtc cacgacggcg cgatcatcgg ggtccagggc
      961 gggatcgagg agctgcgcgc caaggccgag ctctgcctcc gatccggcgc gaaggtcgcc
     1021 ggcttcctga tcaaggactg gaacggggcc aaggccgggc ccaagcggag cgcccgcgac
     1081 tggacctggg acagcgccct ctatcccgcc ctgccccgcg agttggccgc cctcagggcg
     1141 aaggggctgc gggtcctcgc cagcgtcagc cccatgctcc ccgtcgactc ttcacagtac
     1201 gccgaggcct cggcggcggg tcactgcgtg aaggcccccg acggtcggaa ctacacgatg
     1261 ggcgcccccg gccccgtctc gggcatgctc gacctcacga gccccctcgc cctcgcctgg
     1321 gcgaaggagc tcctcaagcg cgagctcctc ggcatcggga tgtcgggctg ggtggccgag
     1381 ccgggcgagt gcctcccgcc cgacgccgtc ctctcctccg gcgaggacgc atcgctcgcg
     1441 cacaaccgct ggcccgtgct ctgggccagg gccaaccgcg aggccatcga ggaggcgggg
     1501 ctcctgggcg aggccctctt cctctcgcgc acgggctggc tcggatcgac gcgttatgcg
     1561 atggcgtttt gggtcgggga cggctccgac gggaggtcca agggggccgg cttcgcgggc
     1621 tcgatacccg ccggcgtctc ggccgggctc tcgggcgtcg gcttctggca gttcgacgtc
     1681 gccggggccc tgtcctccgc gcgcgcgagg ggaggctctg agggcctggt ccggcgcatg
     1741 gagatggcgg ccttcacccc cttcttcgtg gccgaggacg agggcggctc ggggtcctgg
     1801 gccgggccgg agtcccttgc gatgctggcc cggatgaccg aggtcttcgc cgcgctcaag
     1861 ccctaccatc tcgcggtcgc cgccgagtac cgtgactccg gccttcccac gatccgccat
     1921 ccctggatcc actacgaggg ggacgaggcg gtgcagggcc tcagccgcca gtacctgtac
     1981 gggcgagacc tcatggtagc cccatccctg ggcgggagcg aggccctgac gggcctctac
     2041 ctccccgagg acggctgggt tcacctctgg agctcgcgcg agttccgggg cggaagcgtg
     2101 accgtcgaga gcccccccgg ctatcccgcg gtcttctacc gctccgagtc gcgcttcgcc
     2161 tccctcttcg acgcgatacg ccgaacggcg aggcgggtct aggtcccgag ggcaggcgca
     2221 caggcatgag atcggcgctg ctggcggccg gggccgccct cctcggcaac ttcctcctct
     2281 ccctcggcat gaccctgcag aagcgccaca tcgggtggat cggccgcaag ggcccgaggg
     2341 acgcggcctt ccgactcgac ctcctgggct ggctcgccgg cttcgccctc atgaacatcc
     2401 agccggtctt caacgacctc gccctcctgg gtctgccgcc caacgtggtg ggcgcggccg
     2461 cgggcagcag cgtcgccttc accgccatcc tcgcctcggt cctcctccac gagggcctcg
     2521 gcctgcgcag gctcctgtgg accggcgcca tgttcgccgc gatggggctc tcggccttcc
     2581 acgccgaggg ctcgggcacc cgcgtcctcc cccttgccct ctacgtctcg ctggtcccgc
     2641 ccctcatcgc ggccgccctg gccctcctgg ccaggcggag gaggaggggg ccgggcacgg
     2701 cccttgccct cgccgcggtc gccggctccc tcggcggctt catggtcctt cccctggccg
     2761 cccttcggct cgtcggggga ggggacttct cgctctggct cgggacgccc tacctctatc
     2821 tctacattgt cgcgggggtc ggcgccttct cggtggtcca gctcgcctac aaggacgggc
     2881 gcatgtccca ggtcgcgccc gcctactacg gcatgcaggt gctctggccg gccctggcat
     2941 cgtacttcgc gttcgcggcg cccttcgatc cggtgcaggc cgccgccttc gtggccgtgg
     3001 ccctctgcgt ggtggccatc gcggccgagc gctgaagctc cctagaagcc ggggcgcctg
     3061 aagagggtca cgctcgcgta ggcctcgatc gccctgcctt ccccggcggc gtcgaccccc
     3121 tccttggtct tggccttcac cgagaccgag ccgagctcca tgccgagggc ccgggcgagc
     3181 gaggagcgga tctcgtcccg gctcggcccg agcctgggcc gctcgaggac cacggtgcag
     3241 tcgaggttgc caggctccca gcccgaggcg cgcacgaggg acacggcctc gcgcgcgagg
     3301 gccgaggagt cggcgccgcg ccaggcctcg tcgcctgggg ggaagtgcga cccgatgtcg
     3361 cccaacgcgg cggcgcccag gagggcgtcg atcacggcgt ggaagagcac gtcgccgtcc
     3421 gagtgcccgt cctcgccgag ctcgaagggg aggaccacgc ccccgaccag gagcctgcgg
     3481 ttgggcacga gcctgtggag gtcccaaccc tggccgatgc ggaaggggag ggcggctggg
     3541 gtaggcaggt cctcgcggcg ggtcagcttc cggttgcccg gctcccccgg caccgaggcg
     3601 acggcgccct tgtatcgggc ccagagctcg gtgtcgtcgg tgcaggcata gccctcggcc
     3661 gcggccctgc ggtgggcctc gaggaggccg ccgaagcgga aaccctgggg ggtctgggcg
     3721 gcggcgaggt cggagcgccg gggatggccc gagacgaggc ccgcggcgtc gatctgtttc
     3781 ggcgtgtcga cgaggggcac gacgggtacg gccgccccgc gttccagagc cgcctgggcg
     3841 accctggcgg cgaggtcccg gctcgcccag ggtctcgccc catcatggac gagtaccacc
     3901 acctcgtcga gggcgaggcc gctaaggcca gggctgagcg cgagggcctc gaggcccatg
     3961 cggacggagt cgcggcggtc gccgcccccc gcggtgacga tgaagctgga cccgaggcgg
     4021 gactcaaggg catcaaggct gtcccgcgag agggaggagc ggacctcctc ctccatgccg
     4081 ggaggcgcgg tgatggcgag ggcgcgcagt ccgtccaggg agaggaaggg cgagacggcg
     4141 cggtcgagga cgctgcggcc ctcgatcctc accagctcct tcttaccgcc gccgaagcgc
     4201 tcgctgcggc ccgctgcggt gaccaggacg gccaggccag ctggctgggg gctcggcatc
     4261 aggcttcggg ccgggcgcga gacatcatgt cttcagccgg gcgccaagcg tcatgtctcg
     4321 agcctagcgc ggataagctc ggcgatctcc tccttggact tgccgaggga gaaggagatc
     4381 tcgtcctgga ggaggttgag cgccgagtcg tagagcttgc gctcgaggat cgggagctcc
     4441 ttgatcttgg acctgtggta gagcgagcgc accaccgagg cgatgtcgag caccgagccc
     4501 ttcttgagca ggtcgagatt catctggtag cggagcttcc agtcggtggg gatgggggcg
     4561 tactcctcgg agatgaaggc cagggccttc tccgcctcgc ccttggggac gatggcgcgg
     4621 atgccgaggt ccagagcctt gtccaccgga accatcacgg tcatgtcgga gaactcaagg
     4681 tagatgatgt agtagaggag cttcgagtcc ttgaagttct ttggttttat atcgacgatc
     4741 ctgccgacgc cctggcttgg atagacgatg cgctgatcaa ccgcgaaatc cgtgggagtg
     4801 cccttgtcca tattgttcca tactctccgg aaatattaaa aaagtcaaat acggtcaggg
     4861 ccgcggggtc ggccgcctcc cagcttgcgc cggtccgagg cctcgggcta gagtcgagcc
     4921 cctggatcgc aagcgcccct ccctcgcgac gatgctcggc ttccccctcc tcatcgtcgc
     4981 cctggtcgct tcggccatcg tcttccgcca cgagctcctc gccatcttca gggacagaga
     5041 cgcgctgagc gcctggatcg gcgcgagggg ggcctgggga gtcctcgcct tcgtgggcct
     5101 ccaggtcgcc caggtggtgg tcttcgtgat ccccggcgag atcgtccagg tcgccggcgg
     5161 ctacgtctac ggcctctggt acgggaccct gctgtcggtc ctgggcatca ccatcggctc
     5221 gctcatcaac ttctacctgg ggcggctcct cgggaggccc ttcgtcgagg ccctcttcga
     5281 ccgggagagg atcgagtcca tagagcgcgt gaccgcctcc ggaaaggccg ccgcgggctt
     5341 cttcctcctc ttcatcatcc ccggcatccc gaaggacgcg ctctgctaca tcgccggtgt
     5401 ctcgggcctc ggctttcccg ccttcttcgg catctcgatg ttcggccgcc ttccgggcat
     5461 cctcggcagc tccttcatgg gcagcgccgc ccacgacagg tccttcgcct cggcaatcgt
     5521 ggtgctctcg gtcgccgccg tcctcttctg catcggcctc ttcttcaaga gcaggatcga
     5581 gtccatcctg gtgaggctgc tgcacggggg caggaggaag tgagggtcca gggccgcagg
     5641 ggccgagggg gcccatgaac cagggcaagc gcgcgagccc cgacgcccgt gagcgcctgc
     5701 tctcgggtct gctccgagtc tgcgcgggcg gaggcctcct cgcctatctg ccgagcgcct
     5761 acctttcggt ggtccagggc tactggtcgg tcttcgtcgc cgacacggtg gcctgtgtct
     5821 acgtcatcct cctcgccctg aggcccgcca tccccgcccg ggtcaagatc gcctccctcc
     5881 tcgtcatctc ctacctcctt ggcctcatgc tcctgttcac caccgggccc ttcggcgccg
     5941 gacacctctt catcttcgcc ttcgtcctcc tcgccgccct cttcggccgc ctgcgcggga
     6001 tcatcgcggc caacgtgctc gccatcgcca cccacgtcgg catcgcggtg gcctcggcgc
     6061 tgaagctcgt cccctggccc cagagcctgt cctcggtcgt cgtgatttcg gccaacttca
     6121 tcctcgtgag cctcgtgctc tccttctcga ccaacttcct catccgtagc ctcgagacct
     6181 cggcgggcga ggagaggaag ctcaggat
//
LOCUS       DASSQV010000159         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1406684, whole genome shotgun sequence.
ACCESSION   DASSQV010000159 DASSQV010000000
VERSION     DASSQV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406684"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFL04_02800"
     CDS             <1..906
                     /locus_tag="VFL04_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET7838663.1"
                     /translation="NCAVLVSDYDFDRREIADAMVQAKTRGCDVRLVTDGDTIAKADA
                     DLTKTRSQIDPAYHEAFTALRTAGIEIHHDGKRGAIMHDKFAVVDGLAVWTGSWNFTT
                     NDSYRYDNAGIAIESAELAAAYTATFDRMFERGEFGPKRGGSGPRRRVLVEGSPVEVW
                     FSPEDGTEARLVELLGSARTRIDFLAFSFTDDEIGAAVRERAAAGVAVRGVFEKTGSL
                     TKYSEYGAMRKAGLKVLQDGNAYLMHIKAFIVDGRYVAFGSYNFTKNARTENDENLLV
                     VDDVGMAKAFEAEFERVWARAEGAR"
     gene            complement(944..1939)
                     /locus_tag="VFL04_02805"
     CDS             complement(944..1939)
                     /locus_tag="VFL04_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838664.1"
                     /translation="MSGRPASHVPARGSPPSLLLGATAFCSLLLGCAAPLVVEREIDP
                     LAILGPGALVYARLDGVAARSLAPAMLPPDQGKALAPLLKRTESMAFAIGARDSAGPA
                     ADKAKPGGAEAVRPDGDPKGGALPALDAVFLGDYPFRAASLVLGSQPGWRREGQAFAN
                     AATGIRAAVPGPAVMLASTGDYDDLLARVKTPPGSPIPRRLEALSSKELMVWVPEPFK
                     RLAALFVGESMGIPARGLLVSATRKPGAPGAEAVYVVTVAFAMTDANSARIFRPAFRL
                     AWYLIARTLLTEEADAALALHFSLDGELYWATGLELHASTLAASLGRLRGSMGTP"
BASE COUNT          328 a          678 c          767 g          279 t
ORIGIN      
        1 aactgcgcgg tgctcgtctc cgactacgac ttcgatcgtc gcgagatcgc cgacgcgatg
       61 gttcaggcga agacgcgcgg ctgcgacgtg cgcctcgtca ccgacggaga caccatcgcg
      121 aaggccgatg ccgacctcac caagacgcgc tcgcagatcg accccgccta tcacgaggcc
      181 ttcacggctc tccgtaccgc cggcatcgag attcatcacg acggcaagcg cggcgcgatc
      241 atgcacgaca agttcgccgt cgtggacggg ctggcggtct ggaccgggag ctggaacttc
      301 acgaccaatg acagctaccg ctacgacaac gcggggatag ccatcgaatc ggccgagctc
      361 gccgccgcct acaccgcgac cttcgacagg atgttcgagc ggggcgagtt cgggcccaag
      421 cgcgggggca gcggaccgag acggagggtc ctcgtcgagg gctcccccgt cgaggtctgg
      481 ttctcgcccg aggacgggac cgaggccagg ctcgtggagc tcctgggctc ggcgaggacg
      541 aggatcgact tcctcgcctt ctccttcacc gacgacgaga tcggcgccgc ggtgcgcgag
      601 cgggccgccg ccggggtcgc cgtgcgcggg gtcttcgaga agaccggttc cctgacgaag
      661 tacagcgagt acggcgcgat gaggaaggcc ggcctcaagg tcctccagga cgggaacgcc
      721 tacctcatgc acatcaaggc cttcatcgtg gacggccgct acgtcgcctt cggatcctac
      781 aacttcacga agaacgcgag gaccgagaac gacgagaacc tcctcgtcgt cgacgacgtg
      841 gggatggcca aggccttcga ggccgagttc gagcgggtct gggcaagggc cgagggggcc
      901 cgctaggccg agtgggcctg ctgaccgatg gcgcccgccg gtcctagggc gtccccatgc
      961 tgcccctgag cctgccgagg gaggcggcga gggtggaggc gtggagctcg aggcccgtgg
     1021 cccagtacag ctccccgtcg agggagaagt ggagggccag ggctgcgtct gcctcctcgg
     1081 tgagcagggt ccgggcgatc aggtaccagg cgagcctgaa ggccggcctg aagatcctcg
     1141 ccgaattggc gtcggtcatc gcgaaggcga cagtgacgac atagacggcc tcggcccccg
     1201 gggctcccgg cttcctcgtg gccgagacga ggaggccgcg ggccgggatg cccatcgact
     1261 cgcccacgaa gagggcggcg aggcgcttga agggctcggg gacccagacc atcagctcct
     1321 tcgagctcag ggcctcgaga cggcgcggga tgggcgagcc cggcggcgtc ttcacccgcg
     1381 caaggaggtc atcgtagtcg cccgtgctcg cgagcatgac ggccggcccg ggcacggccg
     1441 ccctgatgcc ggtggccgcg ttggcgaagg cctggccttc gcgcctccag ccgggctggg
     1501 agccgaggac aagtgaggcc gccctgaagg ggtaatcgcc gaggaacacc gcgtcgagcg
     1561 cagggagggc tccgcccttg gggtccccat cgggccttac cgcctcggcc ccgcccggct
     1621 tggccttgtc ggcggcgggt cctgcgctgt ccctcgctcc tatggcgaag gccatgctct
     1681 cggtccgctt aaggaggggg gcaagggcct tgccctgatc cgggggcagc atggcgggcg
     1741 cgaggctcct ggcggcgact ccgtcgagcc tcgcgtacac gagggcgccc ggccccagga
     1801 tggcgagggg gtcgatctcg cgctccacca cgaggggggc ggcgcagccg agaaggaggg
     1861 agcagaaggc ggtggcgccc aggagaagtg aagggggaga gccccgggcc gggacgtggc
     1921 tcgccgggcg gccgctcaca gataggcccc cttctggagg agggagaggg cgagctcgcg
     1981 gtgggccctg gccgcctcgt tccaggtgtg gagggccgag ttgcggaagc ggccctgggt
     2041 cctcacccgc ct
//
LOCUS       DASSQV010000160         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_910788, whole genome shotgun sequence.
ACCESSION   DASSQV010000160 DASSQV010000000
VERSION     DASSQV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910788"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..2200
                     /locus_tag="VFL04_02810"
     CDS             101..2200
                     /locus_tag="VFL04_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7838665.1"
                     /translation="MRLPVFCGKDCGGGACPLIAVLDGGRVTRIEHNGAAGTHITGCR
                     RGFGLARSHYAADRLRMPLIRTGDRGSGSFREAGWDEALGLVAQGLADVRDRWGHESV
                     LNLSSAGSLGALHSTPALTSRFLDCFGGRTSLQGSYSNGAARFVLPYLLGPDWRRNSG
                     FDPATLRQAGMIVLWGANVLETRMGAELPSRLLEARRRGARIVAIDPRRTQTVERAST
                     QWLPIRPGTDAALMLALLHVLVTEGLVDRGFVAERAVGFERIEAYVLGAEDGLARDPP
                     WASGICGVAEAEIRDLARAWALTKPTMLIPGYSIQRVEAGEESYRLTVALQLATGNFG
                     ARGGSTGSMNNCLPLPRVGKIDPRSRPGGAAVPQLRWPDAILEGRSGGYASDIHAAYV
                     AGSNLANQGADLAKSARAFGRLDFAVCHEMFLTPTARLCDVVLPAASPLEKEDIGLPW
                     AGNYLLYKPRVAASEGQARSDYDIFAELAWRMGFGPVFTEGRSAAEWIESFIAESEVP
                     DPEEFKSLGIYLGPEQERVGLEDFARDPRGHPLATASGKVELASEAYARDTGGSAIPK
                     WRGRAPEPRLPLSLVSPKIGAATHSQGRDPSGKEATGAQSLEINALDARERGISDGAM
                     VRVFNHAGSTRVRARLTEGIMRGVVSLPEGVWSALDGSGEDRTGSPNVLTTTMGGGPS
                     VSAVMHGIGVEVERMPE"
     gene            complement(2260..>2791)
                     /locus_tag="VFL04_02815"
     CDS             complement(2260..>2791)
                     /locus_tag="VFL04_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838666.1"
                     /translation="DFSFRTPVDVKGGVLVETADGRRTVADIGPAISFGEVQRLEATG
                     ASILFNCATGLFDLDWLVPRIEDIGRRLPVRFTDQDKDAGRYSQAEQVTWEVCSLLPG
                     FLAFAVDKFERFIAAKLLAETVLTSGIGRGDPRIGPELAATSQALHAGLERKLSGPYG
                     LSLRDGRWTAKDDERG"
BASE COUNT          402 a          969 c         1026 g          394 t
ORIGIN      
        1 tgcgccgcgg cagggtggtc gaggcggggc ccgcctccga gatcttctcg cggccccagc
       61 acgagtacac caaggagctc ctcgcgacgg cattcgagac atgaggctgc ccgtcttctg
      121 cggcaaggac tgcgggggag gggcctgccc cctcatcgcc gtgctcgatg gcgggcgcgt
      181 cacgcggatc gagcacaacg gcgcggccgg tacccatatc accggctgca ggaggggctt
      241 cggcctcgcg aggtcgcatt acgccgcaga ccggcttcgc atgcccctca tcaggaccgg
      301 tgaccggggc tcggggagct tcagggaggc gggctgggac gaggcccttg gcctcgtggc
      361 gcaaggcctc gccgatgtgc gcgaccgctg gggccacgag tctgtcctga acctgtcctc
      421 ggcgggctcg ctcggcgcat tgcacagcac gcccgccctc acgagccgct tcctcgactg
      481 cttcggcggg cgcacgagcc ttcagggcag ctacagcaac ggggccgccc gttttgtcct
      541 gccctacctc ctcgggcccg actggaggcg gaactcgggc ttcgatcccg cgaccctgcg
      601 gcaggccggc atgatcgtcc tctggggggc taacgtcctc gagacgagga tgggggccga
      661 gctcccgagc cgcctcctcg aggccaggag gcggggcgcg aggatcgtgg cgatcgaccc
      721 gcgccggacc cagacggtgg agagggcatc gacccagtgg ctgcccatac gacccggcac
      781 tgacgcggcc ctcatgctcg ccctcctcca cgtccttgtc accgagggcc tcgtcgaccg
      841 cggcttcgtc gccgagcggg cggttggatt cgagcgcatc gaggcctatg tcctcggcgc
      901 cgaggacggg ctcgccaggg acccgccctg ggcctcgggg atctgcggcg tcgcggaggc
      961 cgagatcagg gacctggccc gcgcctgggc cctgacgaag cccaccatgc tcatccccgg
     1021 ctactcgatc cagcgggtcg aggctggcga ggagagctac aggctcacgg tcgccctcca
     1081 gctcgcgacg ggcaacttcg gggcgcgcgg tggctcgacg ggctccatga acaactgcct
     1141 cccgctcccg cgggtgggga agatcgaccc gcgctcgagg ccgggcgggg cggcggtgcc
     1201 ccagctccgc tggcccgacg ccatcctcga gggtcgctcg ggcggctatg ccagcgacat
     1261 ccacgcggcc tacgtcgcgg gctcgaacct cgcgaaccag ggggccgacc tcgcgaagag
     1321 cgcgagggcc ttcggccggc tcgacttcgc cgtctgccac gagatgttcc tcactcccac
     1381 ggccaggctc tgcgacgtgg tcctgcctgc ggcctcgccg ctcgagaagg aggacatcgg
     1441 cctgccctgg gcggggaact acctcctcta caagccgcgg gtggccgcga gcgagggcca
     1501 ggcccgctcg gactacgaca tcttcgccga gctcgcctgg cgcatgggct tcggcccggt
     1561 tttcaccgag ggccggagcg cggccgagtg gatcgagtcc ttcatcgccg agtccgaggt
     1621 ccccgacccc gaggagttca agagcctggg gatctacctc gggccggagc aggagagggt
     1681 gggcctcgag gacttcgccc gggacccgcg gggccatccc ctggccacgg cctcgggcaa
     1741 ggtcgagctg gccagcgagg cctacgcccg cgacaccggc ggctccgcga tcccgaaatg
     1801 gcggggtcgg gcgcccgagc cacgtctgcc gctcagcctc gtgagcccga agatcggcgc
     1861 ggccacccac tcccagggca gggacccctc gggcaaggag gccacggggg cccagagcct
     1921 cgagatcaac gcattggacg cgagggagcg gggcatctcc gatggggcca tggtcagggt
     1981 cttcaaccac gcgggctcga cccgggtcag ggcccgattg accgagggca tcatgagggg
     2041 ggtggtgtcc ctgcccgagg gagtctggtc ggccctcgac ggctcgggcg aggaccgaac
     2101 gggttcgccc aacgtcctga ccacgaccat gggcgggggg ccctcggtct cggccgtgat
     2161 gcacggtatc ggggtcgagg tcgagaggat gccggaatag ggactcaccg cgggagcgcg
     2221 gaggaggagg catgaagggg ccttggctcg ggggcctggt cagcctcgct catcgtcctt
     2281 cgcggtccag cgtccgtccc tgaggctgag gccgtagggc ccagacagct tgcgctcgag
     2341 gcccgcgtgg agggcctgcg aggtggcggc gagctcgggc cctatcctcg ggtcgccccg
     2401 gcctatgccg ctcgtgagga cggtctcggc gaggagcttg gcggcgatga agcgctcgaa
     2461 cttgtccacg gcgaaggcga ggaagcccgg caggagggag cagacctccc aggtcacctg
     2521 ctcggcctgg gagtagcggc ccgcgtcctt gtcctggtcg gtgaagcgca cgggcaggcg
     2581 gcggccgatg tcctcgatcc ggggcacgag ccagtcgagg tcgaagagcc cggtcgcgca
     2641 gttgaagagg atggaggcgc ccgtggcctc gagcctctgt acctcgccga aggagatcgc
     2701 ggggccgatg tcagccaccg tcctcctgcc atcggccgtc tccacgagga caccgccctt
     2761 cacgtccacg ggggtgcgga agctgaagtc g
//
LOCUS       DASSQV010000161         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2029867, whole genome shotgun sequence.
ACCESSION   DASSQV010000161 DASSQV010000000
VERSION     DASSQV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029867"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..371)
                     /gene="yccX"
                     /locus_tag="VFL04_02820"
     CDS             complement(3..371)
                     /gene="yccX"
                     /locus_tag="VFL04_02820"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF011000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET7838667.1"
                     /translation="MRSSTSPSTTRTSRPGYPSSWGPPLPPRRPEAPGAFRAVISGRV
                     QGVGFRYEALRAAQRLGLTGWVRNLDDGDVEVWAEGEAAALHDFRLWLEKGPPGARVS
                     SVEVQNRSPTSSYDGFSVVF"
     gene            complement(280..651)
                     /locus_tag="VFL04_02825"
     CDS             complement(280..651)
                     /locus_tag="VFL04_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7838668.1"
                     /translation="MSSKGMVLIVEDEDSIRLSLRDFLTRRGYEVLVASDGVGAIKQL
                     LDYEVDLIVSDYRMEVLGGDYWIRFLKKYCLGTRIIVTSGFLRPEFDIPYEVVYKPFD
                     YEDLEARISLILGATPASSPA"
     gene            complement(644..1264)
                     /locus_tag="VFL04_02830"
     CDS             complement(644..1264)
                     /locus_tag="VFL04_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7838669.1"
                     /translation="MPKTSKKVRIFSALEVANLCGVVNQTAINWIRNGYLKAFTTPGG
                     QYRIYAEDLMQFLDSRGMRIPVELEERFKEDVDWSAVLVVDDDAELNDLLKRWFERRL
                     GPYTVHQAFDGFEAGRLLSEKRPGFVVLDIDLPGVNGHALCRRIKEDPVFGKPFIIAM
                     TGLDRPEEGQTILAEGADAFFSKPLDFEAIAASVADFAEKARARDV"
     gene            1397..>2553
                     /locus_tag="VFL04_02835"
     CDS             1397..>2553
                     /locus_tag="VFL04_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HET7838670.1"
                     /translation="MELTEAFISALKIDEIAIMDRIAAELGIRTSQVSAVISLVAEGS
                     TVPFISRYRKERTGSLDEVQVRDSAHKFESYKNLEERRIEVIRGIFALGKLDEALFAN
                     LGKAKTLTEIEDIWLPFKKKKKTRGMLAQERGLEPLAQIMLGESEAAVDEAAPRFVRE
                     VPENPELSVATAREAVQGAMDIIAERVSQESDNRAIVKKSYVSGGFLKVKGVGDEEKK
                     AKSVYQMYWDYSEPLSALKPHRIMAVNRGEREGELEVELQVDEEAASALLQARATISN
                     RYHREAIDDGVRRLLGPAVLREIRGEAADLADGHAITVFSQNLKNLLMQPPIKGTRVL
                     GIDPGIRTGTKCAALDESGKFLDYFLIFQERDPDAAKKAIAEAVKKHSLKL"
BASE COUNT          484 a          814 c          838 g          417 t
ORIGIN      
        1 ggctaaaaca ctaccgaaaa tccgtcatag gaggaagttg ggctccggtt ctggacctcg
       61 accgagctga cccgggctcc aggggggcct ttctcgagcc agagccggaa atcgtggagg
      121 gccgcagcct cgccctcggc ccagacctcg acgtcgccgt cgtccaggtt cctgacccaa
      181 cccgtgagcc cgaggcgctg ggcagcccgg agggcttcgt agcggaagcc gacgccctgg
      241 acccggccgc ttatgaccgc cctgaaggcg ccgggcgcct caggccggcg aggaggcagg
      301 ggtggccccc aggatgaggg atatcctggc ctcgaggtcc tcgtagtcga agggcttgta
      361 gacgacctca taggggatgt cgaactcggg cctgaggaag cccgaggtca cgatgatccg
      421 cgtgcccagg cagtatttct tgaggaagcg tatccagtag tcgccgccga ggacctccat
      481 gcggtagtcc gagacgatga ggtcgacctc gtagtcgagg agctgcttga tggctccgac
      541 cccgtccgag gccacgagga cctcgtagcc cctgcgcgtg aggaagtccc tgagggacag
      601 gcggatcgag tcctcgtcct cgacgatgag gaccatgccc ttgctagaca tcgcgggccc
      661 tcgccttctc ggcgaaatcg gcgaccgagg cggcgatcgc ctcgaaatcg aggggcttgg
      721 agaagaaggc gtcggccccc tcggcgagga tggtctggcc ctcctcgggg cggtcgaggc
      781 cggtcatcgc gatgatgaag ggctttccga acaccggatc ctccttgatg cggcggcaga
      841 gggcatggcc gttcactcca ggcaggtcga tgtcgaggac gacgaatccg gggcgcttct
      901 cgctgagcag gcggccggcc tcgaagccgt cgaaggcctg gtggaccgtg taggggccga
      961 gcctgcgctc gaaccagcgc ttgaggaggt cgttgagctc ggcgtcgtcg tcgacgacga
     1021 ggacggccga ccagtccacg tcctccttga acctctcctc gagctcgacg gggatgcgca
     1081 tgccgcggga gtcgaggaac tgcatgaggt cctcggcata gatcctgtat tgcccgccgg
     1141 gcgtcgtgaa ggccttgagg tagccgttgc ggatccagtt gatcgccgtc tggttgacca
     1201 cgccgcagag gttggccacc tcgagggcgg agaagatcct gactttcttg ctggttttgg
     1261 gcataccctt gtggctcctg agatctataa gcctaattat agcaattata tccagaatgt
     1321 caaacgtagg ccagaagccc aggcggtttt ccccggcata ttgatttttg ccgcgcttga
     1381 ccccattgta cccgccatgg aattgaccga agcgttcatc tccgcactca agatcgacga
     1441 gatcgccatc atggacagga tcgcggccga gcttggcatc aggacgagcc aggtctcggc
     1501 cgtcatcagc ctcgtggccg aaggctcgac cgtgcccttc atcagccgat accgcaagga
     1561 aaggacgggg agcctcgacg aggtccaggt ccgcgactcg gcccataaat tcgagtccta
     1621 caagaacctc gaggagcgca ggatcgaggt gatccgcggg atcttcgccc tcggcaagct
     1681 ggacgaggcc ctcttcgcca acctcggcaa ggccaagacc ctcaccgaga tcgaggacat
     1741 ctggcttccc ttcaagaaga agaagaagac caggggcatg ctcgcccagg agcgcggcct
     1801 cgagcccctc gcccagatca tgctcggcga gagcgaggcg gccgtggacg aggctgcgcc
     1861 ccgcttcgtc cgtgaggtgc ccgagaaccc cgagctctcg gtggccacgg cccgggaggc
     1921 ggtccagggc gccatggaca tcatcgccga gcgcgtgtcg caggagagcg acaaccgggc
     1981 catcgtcaag aagtcctatg tctcgggcgg cttcctgaag gtgaagggcg tcggcgatga
     2041 ggagaagaag gccaaatcgg tctaccagat gtactgggac tattccgagc cgctctccgc
     2101 actcaagccc caccggatca tggccgtgaa ccggggcgag cgcgagggcg agctcgaggt
     2161 ggagctccag gtggacgagg aggcggcctc ggccctcctc caggcccggg ccaccatctc
     2221 caaccgctac caccgcgagg ccatcgacga cggggtgagg cgtctgctcg gccccgcggt
     2281 gctccgcgag atccggggcg aggcggccga cctcgccgat ggccacgcca tcacagtctt
     2341 ctcccagaac ctgaagaacc tcctcatgca gccgcccata aaggggacca gggtcctcgg
     2401 catcgacccg ggcatccgca ccggcaccaa gtgcgccgcc ctggacgaga gcggcaagtt
     2461 cctggactat ttcctcatct tccaggagcg cgaccccgat gcggccaaga aggccatcgc
     2521 cgaggcggtc aaaaagcact ccttgaagct cat
//
LOCUS       DASSQV010000162         4227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_166989, whole genome shotgun sequence.
ACCESSION   DASSQV010000162 DASSQV010000000
VERSION     DASSQV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166989"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1080
                     /locus_tag="VFL04_02840"
     CDS             58..1080
                     /locus_tag="VFL04_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET7838671.1"
                     /translation="MSDTRNVWIFVEQDGGRIADVSLELMSKGRELADTLKSELWAVL
                     CGAKVEGLTAGLIAQGADRVIVAEHPELEPYRTLPYARILCGLIEERKPYILLFGASP
                     VGRDLGPRVASALRSGLTADCTDLQIGDYESKKEGKVFKDLLMQIRPAFGGNLIATIV
                     NPHHRPQMATVREGVMKRGEGDPSRKGQVERITPRFEAADLALKVVSREMRESKVRLK
                     DSAVIVSGGAGVAGAEEFDLIRELAQVLAGEVGASRAAVDAGLIAHEHQVGQTGTTVR
                     PRLYIAAGISGAIQHRAGMDESNKIIAINTDPDAPIFQIANYKIVGDLADVIPMLIAA
                     LREKAK"
     gene            1091..2818
                     /locus_tag="VFL04_02845"
     CDS             1091..2818
                     /locus_tag="VFL04_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7838672.1"
                     /translation="MAENFYLDNPDLRLRMESADLAEIVDLKEKGYSSAAKYRNAPRN
                     YRDAQDNWRLVLEILGDICANVIAPMAPAADEEGATFHGGEVSYSKATDAAIEAFRKS
                     EILGAMLPWEYGGMNLPETVYQAMVEMVSRAEGGLMTIFGLQEIASTINEFGDEEMKA
                     RILPRFSRGEVFGAMVLTEPDAGSDLGAVQTRATLDEKTGTWKINGVKRFITNGNAHI
                     HLVLARSEEGTKDARGLSMFLVERDRTVKIRRIENKLGIHSSPTCELQYTDTPAILVG
                     KRRFGLMRCAMALMNGARLAVAAQARGISEAAYREALRYAGERLQFGKPVSELPAVSR
                     MLVSMKGEIEAARALIFETSCQVDALKAFEAKQEELAAAGLPADPAVVAKKKRASNLA
                     DMLTPMSKYYATEMGNRVCYKAMQIHGGVGYMKEFNAERHYRDVRITNIYEGTSQLQV
                     VAAIGKMVGRGLDVLLDEWAGLEYPAELASLKAALEETTSNFRKCCDALKDKDREVID
                     YFGADLVEMGAVVVNSWLLLRDAIAVPRKRELVRAYAAEHLPRASGLGAAILAGDMSS
                     SASREAILA"
     gene            complement(2883..3236)
                     /locus_tag="VFL04_02850"
     CDS             complement(2883..3236)
                     /locus_tag="VFL04_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET7838673.1"
                     /translation="MGTQINPAKVTTALELPGFRIIRSFGVVRGIVVRSRSILGNLGA
                     AFQTIVGGNITILTELCEKTRNESFGLMLEHAEELGANAVVSFRYDATEVMDGVTEVL
                     AYGTAVLVEPSNQGQ"
     gene            complement(3371..>4227)
                     /locus_tag="VFL04_02855"
     CDS             complement(3371..>4227)
                     /locus_tag="VFL04_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7838674.1"
                     /translation="ASQTPKEATGSHELDLDFDAETRELLFVRDRFDETFTYFDGSTE
                     RRSGFTLIFYAGASPLDRPAAEKTLVAALSEAAAEAGRAAGSGSGAGSGSVQDNPRGT
                     EPGAGSGSAPNAGSGAAEPKPSGGGDKAAPSGAADQATPPGAGPGPLIAGVDDSAFAE
                     TGIDIGSSPQGIVLSIRDLRFVADSDQVLPSELWRLDALASALRAIPDRRLLVEGHSA
                     ATGKSAGELELSVRRAKAVAEALGARGIEASRIAYRGYGSTRPIASNDTEEGRARNRR
                     VEVTVLDY"
BASE COUNT          699 a         1514 c         1404 g          610 t
ORIGIN      
        1 gatcgccgcc ctcgtcgacg aactcatgcg cgaatacatc gtaggctagg cggcaatatg
       61 agcgacacca ggaacgtttg gattttcgtt gagcaggatg ggggcaggat agccgacgtc
      121 agcctcgagc tgatgtccaa gggccgcgag ctcgccgaca ccctgaagag cgagctctgg
      181 gccgtgctct gcggggccaa ggtcgagggc ctcacggccg gcctcatcgc ccagggcgcc
      241 gaccgggtca tcgtcgccga gcaccccgag ctcgagccct accgcaccct cccctacgcc
      301 cgcatcctct gcggcctcat cgaggagcgc aagccctaca tactcctctt tggcgcctcg
      361 cccgtgggcc gggacctcgg gccccgggtc gcgagcgccc tgcgctcggg cctcaccgcc
      421 gactgcaccg acctccagat cggggactac gagtccaaga aggagggcaa ggtcttcaag
      481 gacctcctca tgcagatccg ccccgccttc ggcggcaacc tcatcgccac catcgtgaac
      541 ccccaccaca ggccccagat ggccaccgtg cgcgagggcg tcatgaagcg gggcgagggc
      601 gaccccagcc gcaagggcca ggtcgagcgg atcacgcccc gtttcgaggc cgccgacctc
      661 gcgctcaagg tggtctcgcg cgagatgagg gagtcgaagg tgaggctcaa ggactcggcc
      721 gtcatcgtgt ccggtggcgc cggcgtcgcc ggagccgagg agttcgacct catccgcgag
      781 ctcgcccagg tcctcgccgg cgaggtgggc gcctcccgcg ccgccgtcga cgcaggcctc
      841 atcgcccacg agcaccaggt cggccagacc ggcaccacgg tcaggccgag gctctacatc
      901 gcggcgggca tctcgggcgc catccagcac cgcgccggca tggacgagtc caacaagatc
      961 atcgcgatca acaccgaccc cgacgccccg atcttccaga tcgccaacta caagatcgtc
     1021 ggcgacctcg ccgacgtgat accgatgctc atcgcggccc tgcgcgagaa ggcgaaatag
     1081 ggagaaggac atggcagaga acttttacct cgacaacccc gacctccgcc tccgcatgga
     1141 gagcgccgac ctcgccgaga tagtcgacct gaaggagaag ggctactcct cggccgccaa
     1201 gtaccgcaac gccccgcgca actaccgcga cgcccaggac aactggcgcc tcgtcctcga
     1261 gatcctcggc gacatctgcg cgaacgtgat agcccccatg gcgcccgcgg ccgacgagga
     1321 gggcgccacc ttccacggcg gcgaggtgag ctactccaag gccaccgacg ccgccatcga
     1381 ggccttccgc aagtccgaga tcctcggcgc catgctcccc tgggagtacg gtgggatgaa
     1441 cctccccgag accgtctacc aggccatggt cgagatggtg tcgcgagccg agggcggcct
     1501 catgacgatc ttcggcctcc aggagatcgc ctcgacgatc aacgagttcg gcgacgagga
     1561 gatgaaggcg aggatcctcc cccgcttctc gcgcggcgag gtcttcgggg ccatggtcct
     1621 caccgagccc gacgccggct cagacctcgg cgccgtgcag accagggcca ccctcgacga
     1681 gaagacgggc acctggaaga tcaacggcgt gaagcgcttc atcaccaacg gcaacgccca
     1741 catccacctc gtcctcgccc ggagcgagga gggcacgaag gacgcccgcg gcctctcgat
     1801 gttcctcgtc gagcgcgaca ggacggtgaa gatccgccgc atcgagaaca agctcggcat
     1861 ccactcctcg cccacctgcg agctccagta caccgacacc ccggccatcc tcgtcggcaa
     1921 gcgccgcttc ggcctcatgc gctgcgccat ggccctcatg aacggcgcca ggctcgccgt
     1981 cgccgcccag gcccgcggca tctccgaggc cgcctaccgc gaggccctgc gctacgccgg
     2041 cgagcggctc cagttcggca agccggtcag cgagctcccg gcggtctccc gcatgctcgt
     2101 gtccatgaag ggcgagatcg aggcggcgcg ggccctcatc ttcgagacga gctgccaggt
     2161 cgacgccctc aaggccttcg aggccaagca ggaggagctc gccgcggccg gcctccccgc
     2221 cgaccccgcg gtggtcgcga agaaaaagcg ggcctcgaac ctcgccgaca tgctcacccc
     2281 gatgagcaag tactacgcga ccgagatggg caaccgggtc tgctacaagg ccatgcagat
     2341 ccacggaggc gtgggctaca tgaaggagtt caacgccgag cgccactacc gggatgtccg
     2401 catcacgaac atctacgagg ggacgagcca gctccaggtc gtcgccgcca tcggcaagat
     2461 ggtcggccgc ggcctcgacg tcctcctcga cgagtgggcc gggctcgagt accctgccga
     2521 gctcgcttcc ctgaaggcgg ccctcgagga gaccacctcc aatttcagga agtgctgcga
     2581 cgccctcaag gacaaggaca gggaggtcat cgactacttc ggcgccgacc tcgtcgagat
     2641 gggcgccgtc gtcgtcaact cctggctcct cctccgcgac gccatcgccg tcccgcgcaa
     2701 gcgcgagctc gtgcgcgcct acgccgccga gcacctgccg cgcgcgagcg gcctcggcgc
     2761 cgccatcctg gcgggagaca tgagctcctc ggcctcgcgc gaggctatcc tggcctaagg
     2821 gccggagggg accgctgggt cgaaggggct gtccggggag cgatccctgg gcagccccgt
     2881 tcttattgac cctggttcga gggctcgacg aggacggcgg tgccgtaggc caggacctcg
     2941 gtgaccccgt ccatgacctc ggtggcgtcg tagcggaagg agaccacggc gttggcgccg
     3001 agctcctccg cgtgctcgag catgaggccg aaggactcgt tgcgggtctt ctcgcagagc
     3061 tctgtaagga tcgtgatgtt cccgcccacg atggtctgaa aggccgcccc caggttgccg
     3121 agtatgctcc tcgacctgac gactatcccc ctgaccacgc cgaaactgcg gatgatcctg
     3181 aagccgggaa gctcgagggc cgtggtgacc tttgccgggt tgatctgagt gcccatgtcg
     3241 cgcaacctcc acctgatgtc tgcgttctgc gtcgactatc gcaggtttcg cccgagggca
     3301 caacgcctcg ggccggggca tgcggcctcg ggccgggaca ggcggcctcg ggccccccgc
     3361 cccgcgggtc tcagtagtcg aggacggtga cctcgacccg gcggttcctg gccctcccct
     3421 cctcggtgtc gtttgacgcg atcggcctcg tcgagccgta gccgcggtag gctatccgtg
     3481 aggcctcgat gccgcgcgct ccaagggcct cggccacggc cttcgccctc cgcaccgaga
     3541 gctcgagctc accggcgctc ttccccgtgg cggcgctgtg gccctccacg agcagtctcc
     3601 tgtccggtat ggccctcagg gccgaggcga gggcgtcgag gcgccagagc tccgagggga
     3661 ggacctggtc ggagtcggcg acgaaacgga ggtccctgat gctgaggact atcccctggg
     3721 gagagctccc gatgtcgatg ccggtctcgg cgaaggcgga atcgtcgacg ccagcgatga
     3781 ggggacctgg gccggccccg gggggcgtcg cctgatcggc ggcgccggag ggcgcggcct
     3841 tgtcgccgcc gccggaaggc ttgggctcgg cggcgccgga gccggcgttt ggggccgaac
     3901 ccgagccggc gcccggctcg gtgccgcgcg gattgtcctg gaccgagcca ctgccggctc
     3961 ccgaacccga gccggccgcc cttccggcct cggcggcggc ttcggagagg gccgccacca
     4021 gggtcttctc ggccgccggt ctgtcgaggg gggatgcccc ggcgtagaag atcagggtga
     4081 agcccgagcg gcgctcggtg ctcccgtcga aataggtgaa agtctcatcg aagcggtcgc
     4141 ggacgaaaag gagctcgcgg gtctcggcgt caaaatcgag gtcgagctcg tggctgcccg
     4201 tggcctcctt cggggtctgg ctggcgg
//
LOCUS       DASSQV010000163         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_289570, whole genome shotgun sequence.
ACCESSION   DASSQV010000163 DASSQV010000000
VERSION     DASSQV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289570"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VFL04_02860"
     CDS             complement(<1..1104)
                     /locus_tag="VFL04_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7838675.1"
                     /translation="MRIGSALLIAWRSIAGRKQAGTSPRGRRYLRGAVLGVALSLVPL
                     VVVLVVADGMIEGITARYLELGTYHLQASPFTMSGVMSIDSQAAALRALPGVVAVYPE
                     RQGPAVAISGSRTAGAAIRAIDPAFLRDPGTLSYLRLSSGSLDLQAGNSILLGEALAR
                     TLAVHVGESVTIVTARQSFSESGEGPSLAPKISIFTVKGIVSAGYRELDALWAFVGSK
                     AAARILSPDGSRSFIGVKVREPFSDLEPVRARIRASLPPEWTVETWSEVERNLFKSFA
                     TTRALLLLVMALTVAVAAINVSSALIMLVLERRRDIAILKSTGAGEGFIWTAFVLAGL
                     ATGGAGTVLGLAAGCLVAWRVNDLIAVTELILNG"
     gene            complement(1101..1778)
                     /locus_tag="VFL04_02865"
     CDS             complement(1101..1778)
                     /locus_tag="VFL04_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7838676.1"
                     /translation="MNEPLIRCTELGKTYSGAEELVVLDGLDFEVERGKSVAIMGASG
                     SGKSTLLSILGGLDRASKGRVAVGEWELSSLPERSLADYRASVVGFVFQFHYLLKDFT
                     ALENVALPAYIKGTPKKEAFDRAESLLRDMGLAERFAHFPSQLSGGERQRAAIARALI
                     NDPSVVLADEPTGNLDSSSARSIRKILFSLCQLYGSTLVLVTHDQQLAGEADLRYELA
                     EGRLRPS"
     gene            complement(1771..>2074)
                     /locus_tag="VFL04_02870"
     CDS             complement(1771..>2074)
                     /locus_tag="VFL04_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7838677.1"
                     /translation="VVGLLLGLLLAVNINEVFSMAEILVNALSTLANHIAGSISGGDF
                     RIFSPNYFYLMEVPVRVLLPETVFIFCASVASAVVAAASATAQVSKLVPSEVLRYE"
BASE COUNT          385 a          665 c          701 g          323 t
ORIGIN      
        1 cccgttcagg atgagttcgg tcaccgcgat gaggtcgttg accctccagg cgaccaggca
       61 gcccgcggcg agcccaagga ccgtgcctgc gccgcccgtg gccagaccgg cgaggacgaa
      121 ggctgtccag atgaaaccct caccggcacc ggtgctcttg aggattgcga tgtcccgcct
      181 ccgctcgagg acgagcatga tcagggcgga gcttacgttg atggcggcga ccgcgaccgt
      241 gagcgccatg accagaagca gcagtgcccg ggtcgtggcg aaggacttga agaggttccg
      301 ctcgacctcg ctccaggtct cgaccgtcca ttccggcggc agcgatgccc gtatcctggc
      361 acgaaccggc tccagatcgc tgaagggctc cctgaccttg accccgatga agctgcggga
      421 gccgtccgga gacaggatgc gcgcggccgc cttgctgccg acaaatgccc aaagggcatc
      481 caattcccga tagcccgcgg atacgatgcc cttgaccgtg aagatggaaa tcttcggggc
      541 gaggcttggt ccctcccccg attccgagaa ggactggcga gcggtcacga tcgtcacgct
      601 ttcgcccaca tggacggcga gagtccgcgc gagggcctct cccaggagga tgctgttgcc
      661 cgcctgcagg tcgagcgagc cggaggagag cctcaggtag ctcagggtgc caggatcgcg
      721 cagaaaggcg ggatcgatcg cgcgtatcgc ggccccagcc gtccgtgagc cagagatggc
      781 gacggcgggc ccctgacgct ccggatacac ggccacgacc ccagggaggg cccgcagcgc
      841 ggcggcctgg ctgtcgatgg acatgacccc gctcatcgtg aagggggaag cctgaagatg
      901 gtaggtcccg agctccagat agcgcgcggt gatgccttcg atcatcccgt cggcgacgac
      961 caggaccacc acgagcggca cgaggctgag ggccaccccc aggacggcgc cgcggaggta
     1021 gcgcctcccc cgtggcgagg ttccggcctg tttccttccc gctatcgacc tccaggcgat
     1081 aaggagagcc gaaccgatcc tcacgatggc ctcagccggc cttcggcgag ctcatacctc
     1141 aggtctgcct ccccggcgag ctgttggtca tgcgtcacga ggacgagggt cgacccgtag
     1201 agctggcaga ggctgaagag gatcttccgg atcgagcggg cgctcgagga gtccaggttc
     1261 cccgtcggct catcggcgag cacgacggaa ggatcgttga tcagggcccg ggctatcgcc
     1321 gccctctgcc tctcgccgcc agagagctgg gaggggaagt gggcgaagcg ttcggccaag
     1381 cccatatccc tgagcaggga ttcggcccta tcgaaggcct ccttcttcgg tgtgccctta
     1441 atgtaggcgg gaagggcgac gttctcgagg gcggtgaagt ccttgagcag atagtggaac
     1501 tggaacacga aacccacgac cgaagcccgg tagtcggcga ggctgcgctc ggggaggctc
     1561 gagagctccc attcgcccac ggcgacccgg cccttgctcg ccctatcgag gccgccaagg
     1621 atggagagca gggtgctttt cccgctcccg ctcgccccca tgatcgcgac gctcttgccc
     1681 cgctcgacct caaaatcgag cccgtcgagg accacgagct cctcggctcc cgaataggtc
     1741 ttgccgagct cggtgcagcg gatgagcggt tcattcatag cggagcacct ctgatgggac
     1801 gagcttcgag acctgggctg tagcgcttgc ggccgccacg acggccgagg cgacggaagc
     1861 gcagaagatg aagacggtct cgggcaggag gacgcgcact ggcacctcca tgaggtagaa
     1921 gtagttgggc gagaaaatgc ggaaatcccc gccggagatg ctccctgcga tgtggttggc
     1981 cagggtgctg agcgcgttca ccaggatttc cgccatggag aagacctcgt ttatgttcac
     2041 cgccaggaga aggccgagga gaaggcccac cacg
//
LOCUS       DASSQV010000164         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1408565, whole genome shotgun sequence.
ACCESSION   DASSQV010000164 DASSQV010000000
VERSION     DASSQV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408565"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1324)
                     /locus_tag="VFL04_02875"
     CDS             complement(50..1324)
                     /locus_tag="VFL04_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7838678.1"
                     /translation="MKLSRLVILALCLCAILPLSVQGQAKPTTIIYYLWDDPSLKPLV
                     DAFNASQKDIVVDARYLPSPDYETKITTLLTAKAEMDCYMQKRQSDMFAHYDNGFIEP
                     LDGLLAKTGVNRAAIDSYKDAVTLDGKVVAFPWRGAAYYTYYNKKLFQKAGVPTPDTY
                     VKSGTWTWDKFAEISKKLSSGDGNVYGSTVYIWGSSQLLMEAQRGKPIIDAKGKIDYD
                     NAILRAFSMRKGLEEAKAMWPLIDMKVTKTHYSKQFYDGRAAMLLIGEWFPGFMISGR
                     DKNLLQGYTWNDWAVTRLPCDEKSYSTFGSPTFNHVTSYAKNKEAAFRFIAWMGGPEG
                     AKVAAEAGVLPAMVDPGVKAVLAKAFPDAQSLEYFTEAKKSYPLLMTKYGSRIEAQVN
                     SMIEEYLLGKLSDSDFDARFRDGLKDIIATSN"
     gene            complement(1514..>2352)
                     /locus_tag="VFL04_02880"
     CDS             complement(1514..>2352)
                     /locus_tag="VFL04_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7838679.1"
                     /translation="AAAALGQKLGASIGGYLAPGPRGADELPEARRELVEALRLDYLR
                     PRGSIATAAPAAAPPAPVRAELDLTRIEQAFRLDEVQNALRQLDSLVALLREAADPAL
                     FRYAVTALSVRIPATFGAEAEELLHGGTEGFREALAGCDRLDDAARLLRSAAERLSER
                     GLHHGERRQRELIAKVKDIVEARLSDRALGTAAIASEVGLSSSYLRDLFKKREGIALL
                     EYLGKARLALAKRLLTETPDSVRSICDQAGFISYSYFFTYFKKMTGCTPSEYREGERP
                     RD"
BASE COUNT          360 a          699 c          880 g          413 t
ORIGIN      
        1 ggatggcagg cccgccgggg ctcgcgcccg ggggcccgcc ggggccttcc tagttcgagg
       61 tggcgatgat gtccttgagg ccgtccctga acctggcgtc gaagtcggaa tcgctgagct
      121 tgccgaggag gtactcctcg atcatgctgt tcacctgggc ctcgatcctc gagccgtact
      181 tggtcatgag gagggggtag gacttcttgg cctcggtgaa gtactcgagg gactgggcgt
      241 cggggaaggc cttggcgagg acggccttga cgccggggtc gaccatggcc gggagcacgc
      301 ccgcctcggc cgcgaccttg gcgccctcgg gcccgcccat ccaggcgatg aacctgaacg
      361 ccgcctcctt gttcttcgcg taggaggtca cgtggttgaa ggtcggggag ccgaaggtgg
      421 agtagctctt ctcgtcgcag ggcagacggg tgacggccca gtcgttccag gtgtagccct
      481 ggagcaggtt cttgtcgcgg cccgagatca tgaagccggg gaaccactcg ccgatgagga
      541 gcatggccgc cctgccgtcg tagaactgct tggagtagtg ggtcttggtg accttcatgt
      601 cgatgagggg ccacatcgcc ttggcctcct cgaggccctt gcgcattgag aaggcgcgga
      661 ggatcgcgtt gtcgtagtcg atcttgccct tggcgtcgat gatgggcttg ccgcgctggg
      721 cctccatgag gagctggctc gagccccaga tgtagacggt ggagccgtag acgttcccgt
      781 cgcccgagga gagcttcttc gagatctcgg cgaacttgtc ccaggtccag gtcccgctct
      841 tgacgtaggt gtccggggtg gggacgccgg ccttctggaa gagcttcttg ttgtagtagg
      901 tgtagtaggc agcgccccgc caggggaagg cgacgacctt gccgtcgagg gtgacggcgt
      961 ccttgtagga atcgatggcc gcccggttca ccccggtctt ggcgaggagg ccgtcaaggg
     1021 gctcgatgaa gccgttgtcg tagtgggcga acatgtccga ctggcgcttc tgcatgtagc
     1081 agtccatctc ggccttggcg gtgagcaggg tcgtgatctt ggtctcatag tcgggcgagg
     1141 gcagataacg cgcgtcgacg acgatgtcct tctgggaggc gttgaaggcg tcgaccaggg
     1201 gcttgagcga ggggtcgtcc cacaggtagt agatgatggt cgtgggcttg gcctggccct
     1261 ggacggagag gggcaggatc gcgcagaggc agagtgccag gattacgagg cgagacagct
     1321 tcatatggtc ctccttcttg aattggcttc cgccgcggaa acgccccagg cgatcccgca
     1381 gggtgccacc cccattgaag ccccaaagcc cgaaacgggg tattcggaat tgcgcggaaa
     1441 acttggaaaa gccggagcgg ggaggttcct tatgagccgc cgacttcgtt ccgcccggat
     1501 cggaggggct ttttcaatcc cgcggcctct ctccctcccg gtattcggag ggggtgcagc
     1561 cggtcatctt cttgaagtag gtgaagaaat aggagtagct gatgaagccg gcctgatcgc
     1621 agatcgagcg gaccgaatcg ggggtctcgg tcaggaggcg tttggccagg gccagcctgg
     1681 ccttgccgag gtattcaagg agggcgatgc cctcgcgctt cttgaagagg tcgcgcagat
     1741 agcttgagga gaggcccacc tcggaggcga tggcggccgt gccgagggcc cggtccgaga
     1801 gccgcgcctc gacaatgtcc ttcaccttgg cgatgagctc gcgctgccgc cgctcgccgt
     1861 ggtggaggcc ccgctccgag agccgttcgg cggccgagcg gaggagccgc gccgcgtcgt
     1921 cgagccggtc gcagccggcg agggcctcgc ggaagccctc ggtccctccg tgcaggagct
     1981 cctcggcctc ggccccgaag gtggcgggga tccgcaccga aagggccgtg accgcgtagc
     2041 ggaagagcgc gggatcggcg gcctcgcgga ggagggcgac cagggaatcc agctgccgga
     2101 gggcgttctg gacctcgtcg agcctgaagg cctgctcgat ccgcgtcagg tcgagctcgg
     2161 cccgcaccgg agcaggggga gcggccgccg gcgcggccgt ggcgatggac ccgcgggggc
     2221 gcaggtagtc gaggcggagg gcctcgacca gctcgcggcg ggcctcgggg agctcgtcgg
     2281 ccccgcgcgg ccccggggcg agatagcccc cgatgctggc gccgagcttc tggcccaggg
     2341 ccgcggccgc gg
//
LOCUS       DASSQV010000165         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1285107, whole genome shotgun sequence.
ACCESSION   DASSQV010000165 DASSQV010000000
VERSION     DASSQV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285107"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VFL04_02885"
     CDS             <1..953
                     /locus_tag="VFL04_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HET7838680.1"
                     /translation="PRGPDPVPARTPEGRLIGELRELDGFPLYELVYRARYAPPARTS
                     MRPEASQTARGGFACTCFTALDERGHRVTGRNFDWQRHPVLVLFTSPPGGHASVSLVD
                     LYYLGYSPARSPLEYPAALADAPALPFDGMNDEGLAVGMMAVSHAEGISGGPGRPRVD
                     ELGLIRLLLDRAASVEEAVGILEHYDVVFQAPPVHYFIADRKGNSLVAEYLGGRLRLT
                     RGRGLWQVSTNFIFGEVPPLRWTSSCWRYAKASSVLAAREGRLDSGGSFGLLETVSQS
                     STIWSSAYDQDILGLELALGRDFGRRFAWILTRADSPPEP"
     gene            complement(978..>2112)
                     /locus_tag="VFL04_02890"
     CDS             complement(978..>2112)
                     /locus_tag="VFL04_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7838681.1"
                     /translation="GVYAQDGRALIRSGTAPLSLSSSAVLDAPSLFDIRRSSLVMKRP
                     LGPEIPGMPGLRQGRGLGGGMGGMMRPLPLNPRPPQAPLLGEAANPRPFRPLLAAPRS
                     LWIEYGLGGFWRERGLLLLGALVATAGALALYLVLFGLYRRNLVLSSREAKNRELLQL
                     GEAAGTLVHEIKNPLGIIRVQAASLRRLGSPEASAKADAIEEEVVRLAGLADRIRAFL
                     KGGEGQAVDLDLVPWLRDYVARYSAGPEAASLAGACIALCGLPEKAEVRIDPERLGQA
                     LDNLVANAREASSNGHLPEISLVRRNGHPPLWELEVSDRGIGVPPEIEERIFEPFFTT
                     KTQGSGIGLALARRIARAGGGELNYRPREGGGSVFFIQLPARL"
BASE COUNT          301 a          776 c          737 g          298 t
ORIGIN      
        1 cgcctcgagg ccctgacccc gtcccggcta ggacgcccga ggggaggctc atcggcgagc
       61 ttcgggagct cgacggtttt cccctctacg agctcgtcta cagggcccga tacgcgcccc
      121 cagcccgcac gagcatgcgc ccggaagcat cccagaccgc ccggggcggc ttcgcatgca
      181 cctgcttcac cgccctcgac gagcggggcc accgcgtgac gggccggaat ttcgactggc
      241 agcgccatcc cgtcctcgtc ctcttcacct cccctccggg cggccacgcc tcggtctccc
      301 tcgtcgacct gtactacctc ggctactccc ccgcccgttc ccccctggag tacccggccg
      361 ccctcgccga cgccccggcc ctccccttcg acggcatgaa cgacgagggc ctggccgtgg
      421 gcatgatggc ggtcagccac gccgagggca tctcgggcgg gccggggcgc ccgcgggtgg
      481 acgagcttgg cctcatcagg ctccttctcg accgggcggc cagcgtcgag gaggccgtcg
      541 gcatcctcga gcactacgac gtcgtcttcc aggctccccc cgtccactac ttcatcgccg
      601 acaggaaggg gaactccctg gtcgctgagt acctgggggg caggctcagg ctgacgaggg
      661 gcaggggcct ctggcaggtt tcgaccaact tcatcttcgg cgaggtcccg cccctgcgct
      721 ggacctcgtc ctgctggcgc tacgcgaagg cgagctccgt cctcgccgcc agagaggggc
      781 gcctcgactc ggggggaagc ttcggcctcc tcgagaccgt ctcccagagc agcaccatct
      841 ggtcctcggc ctacgaccag gacatcttgg gcctcgagct tgcgctcggc cgggacttcg
      901 gccggcgctt cgcctggatc ctgacgaggg ctgacagccc gcccgagccc tagccgcggc
      961 cgcccgccga gcccgactca gagccgggcc ggcagctgga tgaagaagac cgagccgccg
     1021 ccctcccggg gccggtagtt gagctccccg ccgccggccc tcgctatcct ccgcgcgagg
     1081 gcgaggccaa tgcccgatcc ctgggtcttc gtcgtgaaga agggctcgaa gatccgctcc
     1141 tcgatctcgg gcgggactcc gatgccgcgg tccgagacct cgagctccca gagcggggga
     1201 tggccgttcc tgcggacgag ggagatctcg ggcaggtggc cattggacga agcctcccgc
     1261 gcgttggcga cgaggttgtc gagggcctgg ccaagccgct ccggatcgat gcgcacctcg
     1321 gccttctcgg gcaagccgca gagcgcgatg caggccccgg ccagggaggc ggcctcgggt
     1381 ccggccgaat agcgggcgac atagtccctg agccagggca ccaggtcgag gtcgacggcc
     1441 tggccctccc cgcccttgag gaaggcgcgg atcctgtcgg ccagcccggc gagcctgacc
     1501 acctcctcct cgatcgcgtc ggccttggcc gacgcctcgg gcgagcctag gcggcgcagc
     1561 gaggcggcct gcaccctgat gatgccgagg ggattcttga tctcgtggac cagggtaccc
     1621 gccgcctcgc cgagttggag gagctccctg ttcttcgcct cgcggctgct caggacaagg
     1681 ttcctccggt agagcccgaa aaggacgaga tagagggcca gggcgcccgc cgtcgccaca
     1741 agagctccta ggaggaggag ccccctctcc cgccagaagc cccccaggcc atactcgatc
     1801 cagagcgagc gaggcgcggc caggaggggg cggaagggcc gcggattcgc ggcctcgccg
     1861 aggagggggg cctggggggg ccgtgggttc aggggcaggg gccgcatcat gccgcccatc
     1921 cccccgccga ggcccctgcc ctgccggagg ccgggcatcc cggggatctc gggaccgagg
     1981 ggccgcttca tgacaaggga cgagcgccgt atgtcgaaga gcgagggcgc gtccaggacg
     2041 gcggagctgg agagcgagag gggcgccgtg ccgctgcgga tcagggccct gccgtcctgg
     2101 gcatagacgc cg
//
LOCUS       DASSQV010000166         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_788479, whole genome shotgun sequence.
ACCESSION   DASSQV010000166 DASSQV010000000
VERSION     DASSQV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788479"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFL04_02895"
     CDS             complement(<1..644)
                     /locus_tag="VFL04_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HET7838682.1"
                     /translation="MKKCLFILLALVVAASLFGQNLIGVAMPTQSLQRWNQDGANMKA
                     KLEAKGFKVDLQYANNDINTQIQQLENMVTKGAKVLVIASIDGSVLSDVLKKAHQGGI
                     KVIAYDRLIMNTPDVDYYATFDNFKVGAIQGKYIEGALGLNKGKGPYNIELFAGSPDD
                     NNATFFFNGAMSVIKPYLDSKKLVIPTGQKDFTVIAIQSWESAKAQARMDNLIAA"
     gene            complement(761..1573)
                     /locus_tag="VFL04_02900"
     CDS             complement(761..1573)
                     /locus_tag="VFL04_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7838683.1"
                     /translation="MRFEDIVFVYHLHDERQIAWHGRNHAHAEGEYEFHYFISGEGNF
                     RNGTGTWAIAPGSLHLTPPGVRHQILATNLRRPITYYAVLFRAEGDEELAALLGRATA
                     ALERPAAIGTSHRFFFADLLERQSLGEGDLREAGRHSFMAFLYGLLAGAPSAYGAPEN
                     AHVEKAIAIMQGSIEGDLDLAGLCRRLGLSHEHFVRLFSQRMRMPPMRYFTRLKIEAA
                     RAMLSSTNLGISAISEKLGYASQFGFSRAFKSIAGLSPSEYRARCLQKADFR"
     gene            1686..>2094
                     /locus_tag="VFL04_02905"
     CDS             1686..>2094
                     /locus_tag="VFL04_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HET7838684.1"
                     /translation="MNDIFPTIPRIPYEGPDSDRPLAFKRYDPQARIGGLTAREALRF
                     SMAFWHTMTGNGLDVFGAPTMIRPWDGLGDEMEVARMRMRGLFELTEKLSMPFFCFHD
                     RDIAPEGDSLRETNRRLDEIVSLAKKLMASSPTR"
BASE COUNT          345 a          624 c          737 g          388 t
ORIGIN      
        1 gcggcgatga ggttgtccat cctggcctgg gccttggcgg actcccagct ctggatggcg
       61 atgacggtga agtccttctg gccggtgggg atgacgagct tcttgctgtc gaggtagggc
      121 ttgatgacgc tcatggcccc gttgaagaag aaggtggcgt tgttgtcgtc gggggagccg
      181 gcgaagagct cgatgttgta ggggcccttg cccttgttga gcccgagggc gccctcgatg
      241 tacttgccct ggatcgcgcc gaccttgaag ttgtcgaagg tcgcgtagta gtccacgtcg
      301 ggcgtgttca tgatgagccg gtcgtaggcg atgaccttga tgccgccctg gtgggccttc
      361 ttgagcacgt cggagagcac cgagccgtcg atcgaggcga tgacgaggac cttggcgccc
      421 ttggtgacca tgttctcgag ctgctggatc tgggtgttga tgtcgttgtt cgcgtactgc
      481 aggtcgacct tgaagccctt ggcctcgagc ttggccttca tgttggcccc gtcctggttc
      541 cacctctgga gcgactgggt gggcatggcg accccgatga ggttctggcc gaagagcgag
      601 gcggcgacca cgagggccag gagaatgaat aggcactttt tcacggttcc ctcctttggg
      661 aagcgaagat agcgggctaa ggcgatgata ggggcagacc ggggatacca ctatgggaag
      721 tattgacgtt ccataccaat aattgatatc ggatgcgggt ctagcggaag tcggccttct
      781 ggaggcagcg ggcgcggtac tccgagggcg agagcccggc tatgctcttg aaggcccggg
      841 agaagccgaa ctggctcgcg tagccgagct tctctgatat ggcgctgatg ccgaggttcg
      901 tgctggaaag catggcccgg gccgcctcga tcttgagcct cgtgaagtag cgcatgggag
      961 gcatgcgcat ccgctggctg aagagcctga cgaagtgctc gtgggacagg ccgaggcggc
     1021 ggcagagccc ggcgaggtcc aggtcgccct cgatcgagcc ctgcatgatg gcgatggcct
     1081 tctcgacgtg ggcgttctcg ggggcgccgt aggccgaggg ggccccggcg agcaggccat
     1141 aaaggaaggc catgaaggag tgcctgcccg cctcgcgcag gtcgccctcg cccaatgact
     1201 gccgctccag caggtcggcg aagaagaagc ggtgcgaggt gccgatcgcg gccggccgct
     1261 cgagggccgc cgtcgcccgc cccaggaggg ctgcgagctc ctcgtcgccc tcggcgcgga
     1321 agaggacggc gtagtaggtg atgggcctgc gcaggttcgt ggccaggatc tggtggcgga
     1381 ccccgggcgg ggtgaggtgg aggctgccgg gggcgatcgc ccaggtgccg gtcccgttgc
     1441 ggaagttgcc ctcgcccgag atgaagtagt ggaactcgta ctccccctcg gcgtgggcgt
     1501 ggttccgccc gtgccaggcg atctgccgct cgtcgtggag gtggtagacg aagacgatgt
     1561 cctcgaagcg catggtgcca ccacaatatc aatttcgggt atgttcggtc aaatcccgat
     1621 cttctcgggt caggggagaa ggtctatgat ggtcacagcc cgccttcgag cgcgaggagc
     1681 acgacgtgaa cgatatcttt cccaccattc ccaggatccc ctacgagggg cccgactccg
     1741 acaggccgct cgccttcaag cgctatgacc cccaggcccg gatcggcggc ctgaccgcca
     1801 gggaggccct ccgcttctcc atggccttct ggcacacgat gaccgggaac ggcctcgacg
     1861 tcttcggcgc ccccacgatg atccggccct gggacggtct cggcgacgag atggaggtgg
     1921 cccgcatgcg gatgcgcggc ctcttcgagc tcaccgagaa gctctccatg cccttcttct
     1981 gcttccacga ccgggacata gcccccgagg gcgacagcct ccgcgagacc aaccgccgcc
     2041 tggacgagat cgtgtccctg gcgaaaaagc tcatggcctc gagcccgacc cggc
//
LOCUS       DASSQV010000167         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2280732, whole genome shotgun sequence.
ACCESSION   DASSQV010000167 DASSQV010000000
VERSION     DASSQV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2280732"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFL04_02910"
     CDS             complement(<1..444)
                     /locus_tag="VFL04_02910"
                     /inference="COORDINATES: protein motif:HMM:NF023393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HET7838685.1"
                     /translation="MPSRNPVYEALVLRSRESPAGDRILTLMSSELGLVDAFVFGGPK
                     SRLRSLASPYASGRAFVYLDPVKDFRKLSDFEVIDSFPAIREDLDRLWAAGLVAEILI
                     KTSGGGGDYPEVFALARDTLHGLDASPPGRAAYPIVLFCWRLVYLL"
     gene            541..1329
                     /locus_tag="VFL04_02915"
     CDS             541..1329
                     /locus_tag="VFL04_02915"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin ligase family protein"
                     /protein_id="HET7838686.1"
                     /translation="MLIAAIVLTVSAIVLLIIGRVMAGKAALIAGTETVKAAVIEADC
                     RSVAAEIGAGSFAKYLEVKGTTACASPLVAEFSGTSCVRYETTVVREYEESYVETDSD
                     GKSRTGTRRGSETVSSSSRSCPFFVEDGSGRIEVDPTGAEFHLETTVSRFEPGEGERT
                     VGSYVLRAIISGAGGRRTIGYRFTEKCFPVGRQAYVLGEATDAGGALRIRKSEERGRR
                     FIVSLKSEEELVRSARLGALWLTISAGISIAGAIAFVVLDLLRK"
     gene            1354..2019
                     /locus_tag="VFL04_02920"
     CDS             1354..2019
                     /locus_tag="VFL04_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7838687.1"
                     /translation="MLRYLLLDLDNTLYADELGMERDILGRMNEFVAKHLGCSQAEAR
                     EFRHERVRRYGTTLEWLMAEEGFSDPEAYFAAIHPEGEEYCIKGDPRLGAILDSIDLP
                     KAIFTNSPREHAERVLRKLGVGDRFEAIYDIRFCSLKGKPRAEAFRQVCAACGSAPEE
                     TLFVDDLPKYVQGFLAVGGVGILLDEHGRHGEAGLRRIHSLAELPDLLAEEAAEASQL
                     SLF"
     gene            complement(2016..>2300)
                     /locus_tag="VFL04_02925"
     CDS             complement(2016..>2300)
                     /locus_tag="VFL04_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838688.1"
                     /translation="PPLFTDAELAGILSPLLLIVGGRDIMLDSRRGAARLSRLLPTAR
                     VELVPEAGHALIGYGAEIAAFLDDALAAPEGLGAAVGASGLGGPDAGRAG"
BASE COUNT          367 a          813 c          796 g          324 t
ORIGIN      
        1 gaggaggtac acgagcctcc agcagaagag gacgatggga taggccgccc gtccgggcgg
       61 cgaggcgtcg aggccatgga gggtgtcgcg ggcgagggcg aagacctcgg ggtagtcccc
      121 gcccccgccc gaggtcttta tgaggatctc ggcgacgagg ccggcggccc agagcctgtc
      181 caggtcctcg cgtatggcgg ggaaggagtc gatcacctcg aagtcggaga gcttgcggaa
      241 gtccttgacg gggtcgaggt agacgaaggc cctgcccgag gcgtagggcg aggcgaggga
      301 gcgcagcctg ctcttggggc cgccgaagac gaaggcgtcg accaggccga gctcggagct
      361 catgagcgtg aggatccggt cccccgcggg gctctccctc gaccggagga cgagggcttc
      421 gtagacggga ttccggcttg gcacgttgga agtatcgggc cccccgacgg ccatagtcaa
      481 gaccggccat tgcgcggccc ccgcggcgag gctagtcttg gaggaaccgg gaggtcagcc
      541 atgctcatag ccgcgatcgt cctcacagtc tccgcgatcg tcctcctcat catcgggcgg
      601 gtcatggccg gcaaggcggc gctcatcgcg ggcacggaga cggtgaaggc cgccgtcatc
      661 gaggccgact gcagatcggt cgccgccgag atcggcgcgg gctccttcgc gaagtacctc
      721 gaggtcaagg gcaccacggc ctgcgcctcc cccctcgtcg cggagttctc gggcacgagc
      781 tgcgtccgct acgagacgac ggtcgtcagg gagtacgagg agagctatgt cgagaccgac
      841 tccgacggca agagccggac gggtacccgc cgtggctcgg agacggtctc atcgagctcg
      901 cggtcctgcc ccttcttcgt cgaggacggc tcggggcgga tcgaggtcga ccccacgggg
      961 gccgagttcc acctcgagac cacggtctcg cgcttcgagc cgggggaggg cgagaggacc
     1021 gtcggatcct acgtcctccg cgccatcatc tcgggcgccg gggggcggag gaccatcggc
     1081 taccgcttca cggagaagtg cttccccgtc gggcgccagg cctatgtcct cggcgaggcc
     1141 accgacgccg gcggggccct gcggatccgc aaatccgagg agcgcggcag gcgcttcatc
     1201 gtctcgctca agagcgagga ggagctcgtc cgctcggccc gcctcggcgc cctctggctc
     1261 accatcagcg ccgggatttc catcgccggg gccatagcct tcgtcgtcct ggacctgttg
     1321 aggaagtagc gcgagccgtg gtatcacggc gtcatgcttc gctacctcct cctcgacctc
     1381 gacaacaccc tctacgccga cgagctcggc atggagcgcg acatcctcgg gcgcatgaac
     1441 gaattcgtcg ccaagcacct gggctgctcc caggccgagg cgagggagtt ccgccacgag
     1501 cgcgtgaggc gatacgggac caccctcgag tggctcatgg ccgaggaggg cttctccgac
     1561 cccgaggcct atttcgccgc catccacccc gagggcgagg agtactgcat caagggcgac
     1621 ccgaggctcg gcgccatcct cgactcgatc gacctgccca aggccatctt caccaactcg
     1681 ccccgcgagc acgccgagcg cgtgctccgc aagctcggcg tcggcgaccg cttcgaggcg
     1741 atctacgaca tacgcttctg ctcgctcaag ggcaagcccc gtgccgaggc cttccgccag
     1801 gtctgcgccg cctgcggaag cgcccccgag gagaccctct tcgtcgacga cctgcccaag
     1861 tacgtccagg gcttcctcgc ggtgggcggg gtcggcatcc tcctcgacga gcacggccgc
     1921 cacggcgagg ccggcctcag gcggatccac agcctggccg agctgcccga cctcctcgcc
     1981 gaggaggccg ccgaggccag ccagctcagc cttttctagc ccgcccgacc cgcgtccggc
     2041 cctcctaggc ccgaggcccc gaccgccgcg cccagcccct ccggcgccgc cagggcatcg
     2101 tcaagaaagg ccgcgatctc cgccccgtag ccgatgaggg catgtccggc ctcgggcaca
     2161 agctcgaccc gcgccgtggg caggagccgc gagagccttg ccgccccccg cctcgagtcg
     2221 agcatgatgt cgcggccgcc cacgatgagg agaagggggc tcaggatccc ggccagctcc
     2281 gcgtccgtga agaggggagg
//
LOCUS       DASSQV010000168         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1409645, whole genome shotgun sequence.
ACCESSION   DASSQV010000168 DASSQV010000000
VERSION     DASSQV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409645"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VFL04_02930"
     CDS             <1..1029
                     /locus_tag="VFL04_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7838689.1"
                     /translation="REFKGSLVGKDERKDLAVVKFETDAQDISVATLGDSSSLKVGDW
                     AIAIGNPLALMSSVTTGIVSALGRTNGPDNNINDFIQTDASINKGNSGGALLNIRGEV
                     VGINTWIASPTGGSIGLGFAIPINNAKKVIDDLVSKGVVEYGWLGVSLMDIDKATALE
                     LGSDPVKGAFAAHVFKSSPADKGGFLPGDVVLNANGVDITSLNQLVRIVGDLPAGKAA
                     DFTVFRDGKTAKLRVVIEPRSPKVAADDGSLYPGVAVVSLKSEDLDQTKLPKGVKGVF
                     VANVLPKSPAGTVGLQAEDIITAVNDSAVTNVRDFYRLINDPAAKKIAFTVNRGGTVV
                     TTLAYVRK"
     gene            1122..1727
                     /locus_tag="VFL04_02935"
     CDS             1122..1727
                     /locus_tag="VFL04_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET7838690.1"
                     /translation="MHKEFLPYNAVRNNALKMAYRIHQEGVIPDVSYVSLRGGAYMGN
                     VISEYFKIIRKDSRPVFYAAVVARSYSGINEREQVRVDGWTYSPEYLRTGDRVLLVDD
                     IFDSGRTVNHLVEIILAKGLPRKDVKVAVHDYKVCEYRGESLPIQPDYWCRKHVIPTP
                     ENEIWIHYMSHELVGLTEAELRTHYYPEDPELQEILRVLKK"
     gene            1731..>3636
                     /locus_tag="VFL04_02940"
     CDS             1731..>3636
                     /locus_tag="VFL04_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838691.1"
                     /translation="MSAIAWMRAGLARGRAVSAIAALIAALAAAGGQAWPQGTEVVVS
                     GRNLLIPPPSGQARFRFLPSGEWAAFDRTLVLSAAEREERSYQLEVQSGDGQGSVTSY
                     RIDKRPPEAPSAKPAGGLYREALTPTLSAAEGTRIFWTLIGDAAEGQAGFRAYDAGSL
                     PSIALPAAGSALYTLIAYAEDRAGNRSLPARFVYRLADASLPAMAPAADRAPAAPIAA
                     PEIPAPELVTDRGSAVIMAKVQTGKSLLVAVNPGQSPRPEDFAEAVTEGPVSRFSLDC
                     PYGWAGTLNVFYGYRGQDGAILYNPKPLSVALSNPTEAPTAPPPPPAPRLIADPIGRG
                     AFVGFPSYGGEIVAALDDGPEQAYHGPLAVAPGKTRLTVSWYGVDGSGNRSMRRSLGF
                     DLPPPIPEVSLRGAEEGAVLGADASISPTGTATIRYELSYDGSVPNEPDGSSRLLGPS
                     LAIACPPGEARSIVLRYRAFSGPGSEDTGGEGRILRFSIDKRPPSVPSLTESQPAFSD
                     RERSLRLGSDAGASVYVSVSTGSETAPFRLASGPIELVGSDDGPIRYTLRAYAVSRAG
                     IRSAEMAPLVLTVDLNSIYVSDAAPAGGDGSPDRPFSDLDAALALASASGKRRVNLMG
                     RFQLHKAFAAE"
BASE COUNT          601 a         1350 c         1164 g          521 t
ORIGIN      
        1 cgcgagttca agggcagcct cgtgggcaag gacgagcgca aggacctcgc cgtcgtcaag
       61 ttcgagaccg acgcccagga catctcggtc gccaccctcg gggactcctc gagcctcaag
      121 gtgggggact gggccatcgc gatcggcaac cccctcgccc tcatgtcctc ggtcaccacg
      181 ggcatagtca gcgccctcgg gcggacgaac gggcccgaca acaacatcaa cgacttcatc
      241 cagaccgacg cctctatcaa caagggcaac tcgggcggcg ccctcctcaa catcaggggc
      301 gaggtcgtcg gcatcaacac ctggatcgcc tcgcccaccg ggggcagcat cggcctgggc
      361 ttcgcgatcc ccatcaacaa cgcgaagaag gtgatcgacg acctcgtgtc caagggcgtc
      421 gtcgagtacg gctggctggg cgtgtccctc atggacatcg acaaggccac ggccctcgag
      481 ctcggctcgg acccggtcaa gggcgccttc gcggcccacg tcttcaagtc gagccccgcc
      541 gacaagggcg gtttccttcc cggcgacgtc gtcctcaacg cgaacggggt cgacatcacg
      601 agcctcaacc agctcgtcag gatcgtcggc gacctccccg ccggcaaggc ggccgacttc
      661 accgtcttcc gcgacggcaa gaccgcgaag ctccgcgtcg tcatagagcc gcgctcgccc
      721 aaggtggcgg ccgacgacgg cagcctctat cccggcgtgg ccgtggtctc gctcaagtcc
      781 gaggacctcg accagaccaa gctgcccaag ggcgtcaagg gcgtgttcgt ggcgaacgtc
      841 ctgcccaagt cccccgcggg cacggtgggc ctccaggccg aggacatcat caccgcggtg
      901 aacgacagcg ccgtcacgaa cgtccgcgac ttctaccgcc tgatcaacga cccggccgcg
      961 aagaagatcg ccttcaccgt gaaccggggc ggaacggtcg tcacgacgct tgcctacgtt
     1021 cgaaagtgaa actatagtct aggtggccac cgccgccgcc ttccggggcg ccagacaggc
     1081 gccccggaag gcggctattg cgtttcctcc aaggggtcac cgtgcacaag gaattcctgc
     1141 cgtacaacgc cgtccgcaac aacgccctca agatggccta ccggatccat caggagggcg
     1201 tcatccccga cgtcagctac gtgtcgctgc gcggcggagc ctacatgggc aacgtgatca
     1261 gcgagtactt caagataatc cgcaaggact cgaggcccgt gttctacgcc gccgtcgtgg
     1321 cccgctcgta ctcgggcatc aacgagaggg agcaggtcag ggtcgacggc tggacctaca
     1381 gccccgagta cctgcgcacg ggcgaccggg tcctcctcgt cgacgacatc ttcgactcgg
     1441 gccggaccgt caaccacctc gtcgagatca tcctcgccaa gggcctgccg cgcaaggacg
     1501 tgaaggtcgc cgtgcacgac tacaaggtct gcgagtaccg gggcgagagc ctccccatcc
     1561 agcccgacta ctggtgcagg aagcacgtca tccccacgcc ggagaacgag atctggatcc
     1621 actacatgag ccatgagctc gtgggcctca ccgaggccga gctcaggacc cactactatc
     1681 ccgaggatcc cgaactccag gagatcctcc gcgtcctgaa gaagtagccg atgtcggcca
     1741 tcgcctggat gcgcgctggg ctcgcccgag gccgagcggt gtcggccatc gcggccctca
     1801 tcgcggccct ggcggccgcg ggcggccagg cctggcccca ggggacggag gtcgtcgtct
     1861 ccggcaggaa cctcctcata cccccgccga gcggccaggc gcgcttccgt ttcctgccct
     1921 cgggcgaatg ggccgccttc gacaggaccc tcgtgctctc cgccgccgag cgcgaggaga
     1981 ggtcctacca gctcgaggtg caaagtggcg acggtcaggg ctcggtcacc tcataccgca
     2041 tcgacaagcg cccccccgag gcgcccagcg ccaagcccgc gggcgggctc tatcgggagg
     2101 ctctgacgcc gacgctcagc gccgccgagg ggacgcggat cttctggacc ctcatcggcg
     2161 acgcggctga gggccaggcg gggttcaggg cctacgacgc cggctccctg ccgagcatcg
     2221 ccctgcccgc tgcgggaagc gcgctctata ccctcatcgc ctatgccgag gatcgggcgg
     2281 ggaaccggag cctgcctgcc cgtttcgtct atcgcctcgc cgatgcctcc ctgccggcga
     2341 tggcgccggc ggccgacagg gcccctgcgg cccccatcgc cgcgcccgag atccccgcgc
     2401 ccgagctcgt gaccgaccgg ggctctgccg tgatcatggc caaggtccag actggaaagt
     2461 ccctcctcgt cgccgtgaac cccggacaaa gcccgcggcc cgaggatttc gccgaggcgg
     2521 tcaccgaggg gccggtgtcg cgcttctccc tcgactgccc ctatggctgg gccgggacgc
     2581 taaacgtctt ctacggctac cgcggccagg acggggccat cctctacaac ccgaagcccc
     2641 tctccgtcgc cctctccaac ccgaccgagg ccccgaccgc cccgccgccg cccccggccc
     2701 cgcggctcat cgccgacccg atcggaaggg gagccttcgt cggcttcccc tcctatggcg
     2761 gggagatcgt ggcggccctg gatgacgggc ccgagcaggc ctaccatggg ccattggccg
     2821 tcgcccccgg aaagacaagg ctgacagtgt cctggtacgg ggtcgatggc agcgggaaca
     2881 ggtcaatgcg ccggagcctc ggttttgacc tgccccctcc gatccccgag gtgagcctgc
     2941 gtggcgccga ggaaggggcc gtcctgggcg ccgacgccag catcagtccc acgggaaccg
     3001 ctaccatccg ctacgagctg agctatgacg gctccgtgcc gaacgagccc gacggctcat
     3061 cgcggctcct cgggcccagc ctcgccatcg cctgcccccc gggcgaggcg aggtcgatcg
     3121 tgctgcgcta ccgggccttc tcgggcccgg gcagcgagga cacggggggg gagggcagga
     3181 tcctcaggtt cagcatagac aagcgtccgc cctcggtgcc gagcctgacc gagtcccagc
     3241 ccgccttctc cgacagggag aggagcctga ggctcggctc cgacgcgggc gcgagcgtct
     3301 acgtctcggt ctccaccggg agcgagacgg cgcccttccg cctcgcctcg ggccccatcg
     3361 agctggtggg gagcgacgat ggcccgatcc gctacaccct gcgggcctac gcggtgagcc
     3421 gggccgggat caggtcggcc gagatggccc ccctcgtcct caccgtcgac ctcaactcga
     3481 tctatgtctc ggacgccgcg cccgcggggg gagacggctc accggaccgg cccttctccg
     3541 acctcgacgc cgccctcgcc ctggcatcgg cctcgggcaa gcgccgcgtc aacctgatgg
     3601 ggaggttcca gctccacaag gccttcgccg cggaga
//
LOCUS       DASSQV010000169         3402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1658239, whole genome shotgun sequence.
ACCESSION   DASSQV010000169 DASSQV010000000
VERSION     DASSQV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658239"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VFL04_02945"
     CDS             complement(<1..961)
                     /locus_tag="VFL04_02945"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET7838692.1"
                     /translation="MMKEKIDLAAARRCAVAASGLGGLDEGGADCLGAIRRLGSIQID
                     TISVVERAHHHILWPRAQAYAPAHLEELETEPRRIIEYWSHAAAFLPIEDYRFGIPRM
                     ERVKANGHDWFQADPGAIKFVLERIAAEGPLRVQDFERGGKKRAGWWDWKPAKVALEY
                     LFHAGELVSLRRQGFQKVYELASRGLPPGLDLRRPGMEELAAHHVDRAVDSLGVFAAD
                     EVAYLRKEGREGIAAELAARLEAGRLVELVIDGAASESGRSNQRRKYYAAPSSLESPR
                     LPATAFPGRGRNRAFILSPFDPLIIDRRRTARLFGLDYQLECYV"
     gene            1060..1545
                     /locus_tag="VFL04_02950"
     CDS             1060..1545
                     /locus_tag="VFL04_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7838693.1"
                     /translation="MTRAEKLDLISKFESSYDLVEELISGMSDEALRFAPPIPDAWSI
                     HDFLVHFLDADNSLCFRVRGAIAEPGIEAPVWDEDRWHANLHYEAEDGRRCLELAKGL
                     RRFLAASLRSLVDDDWSGYFFNHPTRGRMDLEAVLAMYREHVAFHVSLIKRNREAWKA
                     D"
     gene            complement(1542..2876)
                     /locus_tag="VFL04_02955"
     CDS             complement(1542..2876)
                     /locus_tag="VFL04_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7838694.1"
                     /translation="MRKIPVVSSDEMLNETIRLECARSDGEFAPIFISDRDAIIEYLK
                     YELPEMKILYFSDPRIDCLGVLREIQEDPWLHYGGIIAIHDNADERVLNEAVRDANII
                     AVLRKRDFDEGIPRLLRIIRQNKQILFQRGIQLHLLKSISGAFVMDNEPLDVTTYTNL
                     ITNYLFNANLIGRDDKEKLHVALLELLVNAIEHGNCRIGFEEKTAWLEKNGDIMNLIR
                     EKNRDAEVKARKVYFSYTITPDRTRITIRDEGEGFDWRSRLNEEPDQPGLHGMGMKMA
                     RLYVKDLAYNELGNEVSFEIEHRHGEANAIPGIFASSAETQFSDGEYVCAEGEESDFL
                     YYIVSGTLYVYSKGKLVSSLSPDDLFMGEMSFLLSNRRSATVVSKGKSTLIRISKHDF
                     VNLIKENPHYGIFLARLLAQRLARLNARTARLNTEYLKLKAALGERDPRVQP"
     gene            2997..3070
                     /locus_tag="VFL04_02960"
     tRNA            2997..3070
                     /locus_tag="VFL04_02960"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3031..3033,aa:Ile,seq:cat)
BASE COUNT          637 a         1004 c         1046 g          715 t
ORIGIN      
        1 ggacgtagca ttcgagctgg tagtccaggc cgaaaagccg agccgtccta cggcggtcga
       61 tgatgagggg atcgaagggc gagagtatga aagctcggtt cctcccccga cccgggaagg
      121 ccgtggccgg gagccggggt gattcaaggc tcgagggagc ggcgtagtat ttgcgccttt
      181 ggttcgatcg acccgattcg gaggcggctc catcgatcac aagctcgacg aggcggccag
      241 cctcgagcct ggcggccagc tcggcggcaa tgccctccct gccttccttg cgcaggtagg
      301 cgacctcatc ggcggcaaat acgccaaggc tgtcgacggc gcggtcgaca tggtgggctg
      361 cgagctcctc catgccgggc ctcctgaggt cgagccctgg tggcagacca cgcgaggcga
      421 gctcgtagac cttctggaaa ccctgacgcc ttagcgaaac cagctcgccg gcgtggaaga
      481 gatactcaag ggccaccttc gcgggcttcc agtcccacca accggctcgc ttcttcccgc
      541 cccgctcaaa gtcctggacg cgaagcgggc cttccgcggc gattcgttcc aggacgaact
      601 tgatcgcccc gggatcggcc tggaaccagt cgtgtccgtt ggccttcaca cgctccatgc
      661 gcggaatgcc gaagcggtag tcctcgatcg ggagaaatgc cgccgcatgg gaccagtact
      721 ctatgatccg tcgaggctcg gtttccagct cctcaagatg ggcaggggca tacgcttgtg
      781 cccggggcca gaggatatgg tggtgggcgc gttccacgac gcttatggta tcgatctgga
      841 ttgagccgag cctgcggatc gcgcccaaac agtccgcgcc gccttcgtca aggccgccca
      901 aaccgctggc ggcgaccgcg catcgacggg ctgcggcaag gtcgatcttc tctttcatca
      961 tgtcgaatgt agccacccgg agcctgctcc acaagataga tgtggcctcg agggcagcga
     1021 ggcgatacaa tctctcatcg acactgcgga ggtgctgaca tgacgcgcgc tgagaagctt
     1081 gacctgatct ccaaattcga gtcctcctac gacctggtcg aggagctgat ttccggaatg
     1141 tccgacgagg cactgagatt cgcgccgccg attcccgatg cctggtccat acacgatttc
     1201 cttgtgcact tcctggatgc ggacaacagc ctctgtttcc gcgtccgagg ggcaatagct
     1261 gagcccggca tcgaagcccc ggtctgggac gaggaccgct ggcacgcgaa cctgcattac
     1321 gaggccgagg acgggaggcg ctgccttgag ctggccaagg gcctccgtcg cttcctcgcg
     1381 gcatcgctcc gatccctggt cgacgacgac tggagcggat atttcttcaa ccatccgaca
     1441 cgcgggagga tggacctaga ggcggtgctg gctatgtatc gcgagcacgt cgccttccac
     1501 gtctcgctca tcaagcggaa cagggaagcc tggaaggccg actagggttg aacccgaggg
     1561 tcgcgctctc cgagcgcggc cttcagtttc aggtattccg tattgaggcg tgcggtgcgc
     1621 gcgttcagcc tcgcgagacg ctgggcgagc agcctggcta ggaatatgcc gtaatgcggg
     1681 ttctccttga tcaggttcac gaagtcatgt ttggagatcc tgatcaaggt gctctttccc
     1741 tttgacacca ccgtcgccga gcgccggttg gacaggagga aggacatttc ccccatgaag
     1801 aggtcgtcgg ggctgaggga ggacacgagc ttgcctttcg aatacacgta gagcgtcccc
     1861 gagacgatgt aatagaggaa gtccgattcc tcgccctctg cgcagacata ctccccatca
     1921 gagaactggg tctcggccga actggcgaaa atcccgggga tggcgttcgc ctcaccgtgg
     1981 cggtgttcga tctcaaagga cacctcattc ccgagctcgt tgtaggcaag gtccttcacg
     2041 tacagcctcg ccatcttcat gcccatgcca tggagcccgg gctgatccgg ctcctcattg
     2101 aggcgggagc gccagtcgaa accctcgccc tcatcccgga tcgttattcg cgtgcggtcg
     2161 ggtgtgatgg tgtaggaaaa atagaccttc ctcgccttga cctcggcatc gcggttcttc
     2221 tcgcggatga ggttcatgat gtcgccgttc ttctcgagcc aggccgtttt ctcctcgaag
     2281 ccgatcctgc agttgccgtg ctcgatggcg ttgacgagca gctcaagaag ggcgacgtgg
     2341 agtttttctt tgtcgtcacg gcctatgagg ttggcgttga agaggtagtt ggtaatcagg
     2401 ttggtgtagg tcgtgacgtc caggggctcg ttgtccatga cgaaggcacc cgagatggac
     2461 ttgagcaggt ggagctggat gcccctctgg aataggatct gtttgttctg ccggataatc
     2521 cggaggaggc gggggatgcc ctcgtcaaaa tcgcgcttcc ggaggacggc gatgatgttg
     2581 gcgtcgcgta cggcctcgtt gagcaccctc tcgtcggcgt tgtcatggat ggcgatgatg
     2641 ccaccgtagt ggagccaggg atcctcctga atctcgcgaa gcacgccaag acagtcgatc
     2701 ctgggatcgg agaaatagag gatcttcatc tcaggcagct cgtacttaag gtattcgatg
     2761 atggcgtccc ggtctgagat gaagatcggg gcgaactcgc cgtcggagcg ggcgcactcg
     2821 aggcggatcg tctcgttaag catctcatcg ctggatacga cgggtatctt acgcataaat
     2881 tcctccgaag cggctctggg atcccctgtc cccgattgtg aagatcggca ggatccggcc
     2941 tccaattgac ttttttgcct gcgtatgtat ctatgtaatg gacttttatg ctcttagggc
     3001 ccatagctca gttggtcaga gctgcggact cataatccgt aggtcgctgg ttcaagtcca
     3061 gctgggccca acatcactgt cccaggaccc tccccacctc agcccctcgc ccgcattctc
     3121 caccgatcca cgagtgcaat ccatatcccc gaaaggaggc cctgttggcg ggcgcgtccg
     3181 cgagtgggga agggcttcct tggcggtgcg acgaatcggc gccgcggggc ctgagccttg
     3241 gctgagccgc gccccgattc cagcggagaa agaccgtcgt ttgttatcag gaatcttgcc
     3301 aagtccggaa ggctcttcga caacttaatg atatttccta tatcctattc agtaattcct
     3361 gctctataat aatggatcga atcactcagg gtctctcctt ca
//
LOCUS       DASSQV010000170         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1286174, whole genome shotgun sequence.
ACCESSION   DASSQV010000170 DASSQV010000000
VERSION     DASSQV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286174"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1840)
                     /locus_tag="VFL04_02965"
     CDS             complement(<1..1840)
                     /locus_tag="VFL04_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HET7838695.1"
                     /translation="MREIGLEGTWRLIRLADGSSRGMELPGDILSALVASGEAADPYW
                     DRNELGLQWIGREDWLIEREFDLDEGFLAHPRIVLDISVLDTVAEIRVNGEPVGGQAN
                     MFRGFRAELGDRLRPGRNHISITIRSPERAAALAASALPYPVPYSAYPVSSPHRNLIR
                     KAQCMSGWDWGPCLMTGGIYDGISLIATGELRIEWLSTKTERAPDGAWKLKVEAGLNA
                     PELLERLGSELEVELELELAGNRALRRLSIPRGDSRASIELLVPGVEDWWPAGYGGQR
                     LYELTLSCRAAGGGPVSHVLRKRIGFRELVVRAEEDEVGRGMVFVANGVEVFCKGANW
                     IPADALPSRWTRERLASLLDSAVQANMNCLRVWGGGRYESDAFYELCDERGIMVWQDC
                     MFSCALYPSSPAFLAEVEAEISHQVRRLKDHPCLALWCGNNEALGAINWYPESKASPA
                     RYIADYDRLTEGVLGRVIRELDPDRTWWPSSPSAGPNDWSDNWHSDSRGDMHFWSVWH
                     EGKPFSDYLGVRPRFCSEFGFQSYPSYRQTLAFCPPDQLNVTSPAMEHHQRHPRGNGL
                     ILETMSRYFRMPKGFRETLYLSQVQQALAIRTAVEYWRSLRPRCMGS"
     gene            1991..>2561
                     /locus_tag="VFL04_02970"
     CDS             1991..>2561
                     /locus_tag="VFL04_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET7838696.1"
                     /translation="MSRISGAEANSTLRIVRAIWHNPRISRVDIAARTGLDKSTVTNR
                     VSRLIELGMVREIAEGSAGLKGGRKPIHLAINPSYGRTIGLEVQVESYTAVVTDLSGE
                     VLGETKGRTRIGPGDFSAVVLGIIEESIGKLCPDPSLLLGIGVGTGGLVDQKKGRIRY
                     SVPLGIDAPIDFVKAVTPRLPVPCYLENDA"
BASE COUNT          429 a          836 c          889 g          407 t
ORIGIN      
        1 ggctgcccat gcagcggggg cggagcgagc gccagtactc cacggccgtc ctgatggcga
       61 gggcctgctg gacctggctc aggtacaggg tctcgcggaa gcccttgggc atcctgaagt
      121 agcggctcat cgtctcgagg atgaggccgt tcccccgggg atggcgctgg tgatgctcca
      181 tcgcggggct cgtgacgttg agctggtcgg gggggcagaa ggccagggtc tgtctgtagg
      241 aggggtagga ctggaagccg aactccgagc agaagcgggg ccttactcca aggtagtcag
      301 agaagggctt gccctcgtgc cagaccgacc agaaatgcat gtcgccccgg ctgtcggagt
      361 gccagttgtc ggaccagtcg ttggggcctg ccgaggggga ggacggccac caggtcctgt
      421 cggggtcgag ctcgcggatc acccgcccga ggacgccctc ggtgaggcgg tcgtagtcgg
      481 cgatgtagcg ggcgggcgag gccttggatt ccggatacca gttgatggcg ccgagggcct
      541 cgttgttgcc gcaccagagg gcgagacagg ggtggtcctt gagccgcctc acctggtggc
      601 tgatctcggc ctcgacctcg gcgaggaagg ccgggctcga tggatagagg gcgcaggaga
      661 acatgcagtc ctgccagacc atgatgcctc gctcgtcgca gagctcgtag aaggcgtcgg
      721 actcgtagcg gccgccgccc cagaccctga ggcagttcat gttggcctgg acggccgagt
      781 cgaggaggga ggcgagcctc tccctggtcc agcgcgaggg cagggcgtcg gcggggatcc
      841 agttggcccc cttgcagaag acctcgacgc cgttggccac gaagaccatg cccctgccga
      901 cctcgtcctc ctcggccctg accacgagct cacggaagcc gatgcgcttc cgcagcacgt
      961 gggagaccgg tccgcccccg gccgcgcggc agctcagagt aagctcgtag agcctctggc
     1021 cgccatagcc cgccggccac cagtcctcca caccagggac aaggagttcg atggaggccc
     1081 tcgaatcccc gcgggggatg ctgaggcgcc gcagcgcccg atttccggcg agctcgagct
     1141 cgagctcgac ctcgagctcg gaaccgagcc tctcaagtag ctcgggggcg ttcaggcccg
     1201 cctcgacctt gagcttccag gccccgtcgg gcgcccgctc ggtcttggtc gagagccact
     1261 cgatcctgag ctccccggta gcgatcaggg atatgccgtc ataaatgcct ccggtcatga
     1321 ggcaggggcc ccaatcccag cccgacatgc actgggcctt gcggatgagg ttccggtgcg
     1381 gggagctgac cgggtaggcg ctgtagggga cgggataggg gagggcggac gcagccaagg
     1441 ccgcggccct ctcgggcgag cggatcgtga tcgagatgtg gttcctgccc ggtctgagcc
     1501 ggtcccccag ctcggcgcgg aagccgcgga acatgttcgc ctgcccgccg acgggctccc
     1561 cgttgaccct gatctcggcg acggtgtcga gcacggagat gtcgaggact atcctagggt
     1621 gggccaggaa gccctcgtcc aggtcgaact ccctctcgat cagccagtcc tcgcggccga
     1681 tccactggag gccgagctcg ttcctgtccc agtagggatc ggcagcctcg cccgaggcga
     1741 cgagggcgct gaggatgtct cccggcagct ccatgccccg gcttgagccg tcggcgaggc
     1801 ggatcagcct ccatgtcccc tcgagcccga tctctctcac gttccctgcc tccgccccgg
     1861 ggcgccggaa tgaagttcgt tgacgtcccg ggatacccgt ggtatacatt gcttggtttg
     1921 taaaatcaac caacccaggc ggggctggcc ccgccaatgc tccactatag cggctgcagg
     1981 gggatccagg gtgtcgagaa tctcgggggc agaggccaat tccacgctga ggatcgtgag
     2041 ggcgatctgg cacaatcccc gcatcagcag ggtggacatc gccgcgagga cgggcctcga
     2101 caagtcgacg gtcacgaacc gggtgagccg cctcatcgag cttggcatgg tcagggagat
     2161 agccgagggc tcggcgggac tcaagggcgg cagaaagccc atccacctcg ccataaaccc
     2221 cagctatgga aggaccatcg ggctcgaggt ccaggtggag tcctacacgg ccgtggtcac
     2281 cgacctctcg ggagaggtcc tcggcgagac caagggaagg acccggatcg gccccgggga
     2341 cttctcggcg gtggtcctcg gcatcatcga ggagagcatc gggaagctct gccccgaccc
     2401 ctcgcttctc ctcggcatcg gggtcggcac cggcggcctc gtcgaccaga agaagggcag
     2461 gatccgctac tccgtccccc tcggcatcga cgcgccgatc gacttcgtga aggccgtaac
     2521 accccgtctc cccgtcccct gctacctaga gaacgacgcc a
//
LOCUS       DASSQV010000171         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_291238, whole genome shotgun sequence.
ACCESSION   DASSQV010000171 DASSQV010000000
VERSION     DASSQV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291238"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VFL04_02975"
     CDS             complement(<1..1158)
                     /locus_tag="VFL04_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838697.1"
                     /translation="MRGRGPSGPTASTWADALLTTDAEALLGAVRNYLGPVKTPYDKR
                     DLVKRLESFLRRPDTAESLILLLDRLDARIIGSCLLLGPSSETAVKELFAGELALFEL
                     GVRLSNLLDRLLLFRYVADGKRLIAVNPLLEASLRLTVLDPLLLFGASDRPALGVPER
                     QAACDAPTCVAFFSFLFHTPGALRKGGGLTKRATERVLPLFAGMGAGTAERAAALARA
                     LYASGAIRHEESEWAADRNAFGSMLAQWGPDLPFFLAAALSDLDSHGEDEEREGSLAA
                     AGTGARGRIEGLARLLAEALGSPHVADMSRAGLSRWLRICSRRADHQGDPSLAMPALE
                     ALGLLGLAVAAKARPMAEAGPPAASEPSASEPRLPEAAGSAEPRVRLVAEGA"
     gene            complement(1155..>2753)
                     /locus_tag="VFL04_02980"
     CDS             complement(1155..>2753)
                     /locus_tag="VFL04_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HET7838698.1"
                     /translation="ASDFEEARAALGSFAALEKSPEHLHTYRMSALSLWNASSAGLGP
                     DEVEAVLERYSRYEVPKDVLFIVRDTMSRFGRLVLKAAEEEGALLLTCADELAEAELA
                     SSKRLAKWLEPVPGGFLIRLTDRGTVKRELLRLGWPVKDEAPLVPGDPLELRLLDSRR
                     SGGDFCLRPYQTDALAAFVGGGLPGSGYGVVVLPCGAGKTIVAMAAMSAIGRKTLIVT
                     TNVAAVHQWMDELADKTDLPPESMGEYTGAEKTVRPVTIATYQILTWRPDKESDFPHF
                     ELFRKEKWGLVIYDEVHLLPAPVFRVVAEIQAVRRLGLTATLVREDGREDDVFSLIGP
                     KRYDVPWKDLESKGFIAEAYCREIRIPLPEHDHIRYTVAEPRDKHRLAAENPAKYEAV
                     RELVANHPEEPVLVIGQFLDQLGRIAKELGAPIITGKTPNPERERIYAEFKAGIVRVI
                     VVSKVANFAIDLPDASVAVQVSGSFGSRQEEAQRLGRILRPKARNSYFYSLVSRYTVE
                     EEFAANRRKFLIEQGYRYHIEAWE"
BASE COUNT          401 a          938 c          987 g          427 t
ORIGIN      
        1 ggccccctcg gccacgagcc tcaccctcgg ctctgctgac cctgccgcct cgggtagcct
       61 cggctcagag gcgctcggct cagacgctgc ggggggcccc gcctccgcca tcggcctggc
      121 cttggccgcc accgcgagcc cgaggagtcc gagggcctcg agggcgggca tcgcgagcga
      181 ggggtcgccc tggtggtcgg cgcgacgcga gcagatcctg agccagcgcg agagcccggc
      241 tcgggacatg tcggcgacgt ggggcgagcc gagggcctcg gcgaggagcc tggcgaggcc
      301 ttcgatccgt ccgcgcgccc ccgtccccgc cgccgcgagg ctcccctccc tctcctcgtc
      361 ctccccgtgg ctgtccaggt cgctgagggc ggccgcgagg aagaagggca ggtcgggccc
      421 ccactgggcc agcatcgagc cgaaggcgtt cctgtccgcc gcccactcgc tctcctcgtg
      481 gcggatcgcc ccggaggcgt acagggccct ggcgagtgcc gcggcccttt ccgcggtgcc
      541 cgcccccatg ccggcgaaaa ggggcagcac ccgctcggtg gcccgtttgg tgagcccgcc
      601 gcccttcctg agcgcccccg gcgtgtggaa aagaaaggag aagaaggcca cgcaggtcgg
      661 ggcgtcacag gcggcttggc gctcggggac ccctagggcg gggcggtcgg atgctccgaa
      721 gagcagcagg gggtcgagga cggtcagcct caggctggcc tcgaggaggg ggttgaccgc
      781 tatcagccgc ttcccgtccg ccacgtagcg gaagaggagg agacggtcca ggaggttgga
      841 cagccgcacc ccgagctcga agagggcgag ctccccggcg aaaagctcct tgacggccgt
      901 ctcgctcgat ggtccgagaa ggaggcagct gccgatgatc cgcgcgtcga gcctgtcgag
      961 gaggaggatg agggattcgg cggtgtcggg ccttcgcagg aatgactcga gccgcttcac
     1021 gaggtcgcgt ttgtcgtagg gggtcttcac cgggccgagg tagttgcgca cagcgccgag
     1081 cagggcctcg gcgtcggtcg tgaggagggc gtcggcccag gtcgaggccg tcggaccgct
     1141 cgggccccgt cccctcattc ccaggcctcg atgtggtagc ggtagccctg ctcgatgagg
     1201 aacttcctcc ggttcgcggc gaactcctcc tcgacggtgt agcgcgacac gagggagtag
     1261 aagtacgagt tgcgcgcctt cgggcggagg atccgcccca ggcgctgggc ctcctcctgc
     1321 ctcgagccga agctgcccga cacctgcacc gccaccgagg cgtcgggcag gtcgatcgcg
     1381 aagttcgcga ccttggagac gacgatgacc ctcacgatcc ctgccttgaa ctcggcgtag
     1441 atccgttcgc gctcggggtt cggggtcttg cccgtgatga tcggggcccc gagctccttc
     1501 gctatcctgc ccagctggtc gaggaactgc ccgatcacga ggacgggctc ttcggggtgg
     1561 ttcgccacca gctccctgac cgcctcgtac ttggcggggt tctcggcggc gagcctgtgc
     1621 ttgtcccggg gctcggccac cgtatagcgg atgtggtcgt gctcgggcag gggtatcctg
     1681 atctcgcggc agtaggcctc ggcgatgaag cccttgctct cgaggtcctt ccagggcacg
     1741 tcgtagcgct tgggcccgat gaggctgaag acgtcgtcct ccctcccgtc ctcccgcacg
     1801 agggtcgccg tgaggccgag ccgcctgacc gcctggatct ccgcgacgac cctgaacacc
     1861 ggggccggaa gcaggtggac ttcatcgtag atcaccaggc cccacttctc cttgcggaag
     1921 agctcgaaat gggggaagtc cgactccttg tctggcctcc aggtgaggat ctggtaggtg
     1981 gcgatggtca ccgggcggac cgtcttctcg gccccggtgt actcgcccat ggactcgggc
     2041 gggaggtcgg tcttgtcggc gagctcgtcc atccactgat gcaccgccgc gacgttcgta
     2101 gtgactatga gggtcttgcg cccgatggcc gacatcgcgg ccatggcgac gatggtcttg
     2161 ccggccccgc agggcaggac cacgaccccg tagcccgagc cggggaggcc gccgcccaca
     2221 aaggccgcga gggcgtcggt ctggtagggc ctcaagcaga aatccccgcc cgaacggcgc
     2281 gagtccagga gcctcagctc gaggggatcc ccggggacca agggagcctc gtccttgacc
     2341 ggccagccga ggcggaggag ctcgcgcttc accgtccccc tgtcggtgag gcggatgagg
     2401 aagccgccgg gcacgggctc gagccatttg gcgaggcgct tggagctcgc aagctcggcc
     2461 tcggccagct cgtcggcaca ggtgaggaga agtgcgccct cctcctcggc ggccttgagg
     2521 accaggcgcc cgaacctcga catcgtgtcg cggactatga acaggacgtc cttggggacc
     2581 tcgtacctcg agtagcgctc gaggaccgcc tcgacctcgt cgggcccgag ccccgcgctc
     2641 gaggcgttcc agagcgaaag ggccgacatc ctgtaggtat gcagatgctc ggggctcttc
     2701 tcgagggccg caaaggagcc gagggcggcc cgggcctcct cgaaatccga ggc
//
LOCUS       DASSQV010000172         6751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_291285, whole genome shotgun sequence.
ACCESSION   DASSQV010000172 DASSQV010000000
VERSION     DASSQV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291285"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFL04_02985"
     CDS             <1..718
                     /locus_tag="VFL04_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7838699.1"
                     /translation="SIFGYFTLYMDRRALNAVTGLGPNDCNLLGMYLKDYRSAERDSV
                     RLLAALKEAVSTFPPLASREDFNRERSADYQGIRYGTMTIGGFLSEIGTMLEALTMVS
                     YGILALVIVVVTVGITNIYRVMIYERTREIGTMRAIGVRRSQVRNLILTEALMLSACS
                     IVLGLAASLLALGLVSRFHLDVGAGFDIFLDRGHLSWVINGDVTAVDAALIALVTLCG
                     AFGPARAAQAIEPAVALRSE"
     gene            730..1539
                     /locus_tag="VFL04_02990"
     CDS             730..1539
                     /locus_tag="VFL04_02990"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HET7838700.1"
                     /translation="MALSRIASASFRTSWLALVAGILFLSPAAVRAAGDTDYTQLLRK
                     ADALVTFPDTDFSAEYTFVQEKPGQGKSTKQAMVFRRDKENTYLIVMVMPVEDKGKGY
                     LKSDDNLWFYDPVSRRFTFTSAKERFQNMNARNSDFTRSNLAGDYKVTGAHRESLGKY
                     ECWVLDLLATSDEIPSPKMRIWISDDGLARKTEDYSLSGQKLRTTAFPQYQVVGNRYV
                     PQTVVILDELRGAMINGVFVKERTTYTITRPSMQSLPDATFSKAWLEKLSD"
     gene            1532..2935
                     /locus_tag="VFL04_02995"
     CDS             1532..2935
                     /locus_tag="VFL04_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838701.1"
                     /translation="MTEPSPFRAAGAARRRALGVLVLFLAVGLPALAQATAAPGGDDL
                     DQLFDSGATVPPALPDKGPGATAPVRPEDLTRDDKMHFFGTWSAYANLGPGWSNWPNP
                     DYPKRYFGIDGGASLTAGLGVEVRPAPQLRLRALATYNFPTTGIQVSELIIDYTLRNT
                     VFFRAGIFGYTWGDAQFSQLGNLPGRTLPTWTTNTIPVWQQTNLLTNTTTSTLPTAVK
                     AYIPLGRNGLTFYVRADPNYFETRTEPDLKSAGYGLMYDQVTGPIEWSLGGYYERLLT
                     PRSMLMARSTVFGCALSVEGVMAFPVTYKSTGFEAIPTSGGGLYIGGLMKRVYPDLIV
                     GLSREWPALDLKLHAEYTYNSERDPHGDQANPNWLEDAVGPGGNATDVALHLGHFLHN
                     RKLGLNLLWQHNWSTYAGLTAAFLELAPDSLFTVQIGAETIYGRDIAEVSANRLLPEN
                     NRFGFVILARLNTSFKD"
     gene            3050..3949
                     /locus_tag="VFL04_03000"
     CDS             3050..3949
                     /locus_tag="VFL04_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7838702.1"
                     /translation="MSETSTPAAGRHQGPALRSALRAALCLALLGAGALGPALAEESP
                     QQPQPAQAPQTGLVPDSFPFRLSAVKEAAIGAGGLGIYGSSLYLEARKPSPRASDINA
                     SDIPFFDRLYVTSHPAWMGTAADALMVTAAVVPELAAAAFVPDFSLGKFATIGVMYAE
                     TMGLAYATPNLLKAAITRYRPYAYASTVDLTDSELNSSFPSRHAVIAFSSAVFAGYVF
                     DELAPDSPWRTLVWVSGLGLATATSALRVASGDHFLSDVVAGGVFGAAIGFAVPWMHR
                     TRSTGEGLSLAPLPGGLLVSIGL"
     gene            complement(3963..4793)
                     /locus_tag="VFL04_03005"
     CDS             complement(3963..4793)
                     /locus_tag="VFL04_03005"
                     /inference="COORDINATES: protein motif:HMM:NF042560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6675 family protein"
                     /protein_id="HET7838703.1"
                     /translation="MQERPHSLIRQSLGLGLLLFFLCASAAAQPPEPGLEAARTGCAP
                     FYDDLVAKGKAVRTSDSGSPSLIPAFPGSDLVRAAIAAERPGILVETVFLLPRAAPAE
                     SDRTKMELAAIYGLLRSFSSLEGIQYYSVSHHSMRTLYAESYRIDGPETRRRLPDPPS
                     PSASALPEAESLFAFQRDLSFGQNVYSYAFRGLQGAVMVEVGNVTKMSWGIVPIAAPG
                     ALKTWLFVVQASDAVVFYTASGAKAPGIFRSRLQESFVNRAAALFAWFTAHKEAWLGR
                     "
     gene            complement(4892..6256)
                     /locus_tag="VFL04_03010"
     CDS             complement(4892..6256)
                     /locus_tag="VFL04_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7838704.1"
                     /translation="MKKAFISLIPLVLCTILATLPLRPQAAQQRYDLVSIIEASDLAL
                     TGHDIAKYMTFTTGDFVQDAVASPKPVSRADFSARIGGRLAADPKLYHFQKQILVSGD
                     LAFFDECSFVRTNPLTGLPFRVFHADIVSFENGKMKVMTTFGEGAAEDYALGRIEATL
                     PQPPAPGSAGWPEDPRPTGLKPMAAHLETLERLGKHDMGSLGQMLARSAKVLVSPLFE
                     EVGRDSYVAWMGTLISAFPDLAFKPVRSIDLGGGWVVSELVLSGSNTGSWLGNQATGR
                     PISVRTAYLGHYGSDGLATSLKWYLNSMTILGQLGLKPRPLSTGPAYEKAVEAAIRAN
                     FDRYSSTLMAGDAEGWLALWDEDGVQLPPNEGMHMGKAEIRNLAVVRIPSRPANAFSM
                     KIDTQEVKVEGGLAFARGVYSWSARPKDSGPLSYDGKFLTVFRKQADGSWKIFRDCFN
                     SNLP"
     gene            6398..>6751
                     /locus_tag="VFL04_03015"
     CDS             6398..>6751
                     /locus_tag="VFL04_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838705.1"
                     /translation="MLGIVRIRLLGPPFLETSARRIALSRRKSLALLAWIAVKGPQRR
                     DSAAALLWPESGGASARTNLRSAIFDINTASDAEILALSDDSIGLVPDRVDLDHAAFE
                     AAMASCPRCRRSACCG"
BASE COUNT         1122 a         2380 c         2132 g         1117 t
ORIGIN      
        1 gagcatcttc ggctatttca ccctctacat ggaccgacgc gcgctgaacg cggtcacggg
       61 cctcgggccg aacgactgca acctccttgg catgtacctc aaggactacc gctcggccga
      121 gcgggactcc gtgcggctgc tcgccgccct gaaggaggcc gtgtcgacct tcccgcccct
      181 ggccagccgc gaggacttca accgggagcg ctcggccgac taccagggga tccgctacgg
      241 cacgatgacg atcgggggct tcctctccga gatcggcacc atgctcgagg ccctcaccat
      301 ggtcagctac gggatcctcg ccctcgtcat cgtcgtggtg accgtgggca tcacgaacat
      361 ctacagggtc atgatctacg agcggacgcg cgagatcggc acgatgcgcg ccatcggggt
      421 caggaggtcc caggtgagga acctcatcct caccgaggcc ctcatgcttt cggcctgcag
      481 catcgtgctc ggcctcgcgg cgagcctgct cgccctcggc ctggtctcgc gcttccacct
      541 cgacgtgggc gccggcttcg acatcttcct tgatcggggc cacctgagct gggtcatcaa
      601 tggcgacgtg acggcggtcg acgccgccct gatcgcgctg gttacgctat gcggggcctt
      661 cgggcccgcg agggcggccc aggccatcga gcctgccgtc gccctccgtt cggaatgaca
      721 ggagtggaca tggctctatc gcggatagca tcggcatctt tcaggacctc gtggctcgca
      781 ctcgtagcgg gcatcctttt cctctctcct gcggccgtcc gggccgcggg cgacaccgac
      841 tacacccagc tcctccgcaa ggcggacgcc cttgtgacct tccccgacac cgatttctcc
      901 gccgagtaca ccttcgtgca ggagaagccc ggccagggca agagcaccaa gcaggcgatg
      961 gtcttccgcc gcgacaaaga gaacacctac ctcatcgtca tggtcatgcc cgtcgaggac
     1021 aagggcaagg gctacctcaa gtcggacgac aacctctggt tctacgaccc ggtgtcgcgg
     1081 cgcttcacct tcacgagcgc gaaggagcgc ttccagaaca tgaacgcgcg caactcggac
     1141 ttcacccgct cgaacttggc gggcgactac aaggtgacgg gagcccacag ggagagcctt
     1201 ggcaagtacg agtgctgggt cctcgacctc ctcgccacca gcgacgagat cccctccccc
     1261 aagatgcgca tctggatcag cgatgacggc ctggccagga agaccgagga ctacagcctc
     1321 tcgggccaga agctgcgcac gaccgccttc ccccagtacc aggtggtcgg caaccgctac
     1381 gtcccccaga ccgtcgtcat cctcgacgag ctccgcggag ccatgatcaa cggggtcttc
     1441 gtcaaggaga ggacgaccta cacgatcacg cggccctcca tgcagagcct tcccgacgcg
     1501 accttctcga aggcctggct ggagaagttg agtgactgag ccaagcccct tccgggccgc
     1561 cggggcggct cgccgccgag ccctgggcgt ccttgtgctc ttcctcgcgg tcggccttcc
     1621 cgccctggcg caggccacag ccgccccggg cggggacgac ctggaccagc tcttcgacag
     1681 cggcgccacc gtgcccccgg cactgccgga caagggcccc ggcgccacgg ccccggtccg
     1741 gcccgaggac ctgacccggg acgacaagat gcacttcttc ggcacctgga gcgcctatgc
     1801 gaacctcggg ccgggctggt caaactggcc caaccccgac tatcccaagc gctacttcgg
     1861 catcgacggc ggcgcctcgc tgacggcggg gctcggggtc gaggtcaggc ccgcccccca
     1921 gctcaggctg cgcgccctcg cgacctacaa cttccccacg acgggcatcc aggtctccga
     1981 gctcatcatc gactacaccc tcaggaacac ggtcttcttc cgcgcgggca tcttcggcta
     2041 cacgtggggc gacgcccagt tctcccagct cggcaacctc ccgggtcgga ccctccccac
     2101 ctggacgacg aacaccatac cggtctggca gcagacgaac ctcctcacga acacgacgac
     2161 ctcgaccctc ccgacggcgg tcaaggccta catccccttg gggcgcaacg gcctgacctt
     2221 ctatgtccgc gccgacccga actacttcga aacccgcacc gagcccgacc tcaaaagcgc
     2281 gggctacggc ctcatgtacg accaggtgac ggggcccatc gagtggagcc tcggcggcta
     2341 ctacgagcgc ctcctcaccc cccgctccat gctcatggcc aggtcgaccg tcttcggctg
     2401 cgccctctcc gtcgagggcg tcatggcctt cccggtcacc tacaagtcga ctggcttcga
     2461 ggcgatcccc acctcgggag gcggtctcta catcgggggc cttatgaaga gggtctatcc
     2521 cgacctgata gtcgggctct cgcgcgaatg gcctgccctc gacctcaagc tccacgccga
     2581 gtacacctac aacagcgagc gcgaccccca cggcgaccag gccaacccga actggctcga
     2641 ggacgccgtg gggccaggcg gcaacgccac cgacgtggcc ctccacctgg gtcacttcct
     2701 ccacaacagg aagctgggcc tgaacctcct ctggcagcac aactggtcga cctacgctgg
     2761 ccttaccgcg gcattccttg agttggcccc cgattcgctc ttcacggtcc agatcggggc
     2821 cgagaccatc tacggcaggg acatcgccga agtctcggcg aaccgcctcc ttcccgagaa
     2881 caaccgcttc ggcttcgtga tcctcgcccg gctcaacacg agcttcaagg attaggaaca
     2941 gggcgagcgg tcccccgctg ccccagaggc ggccatttcc gccaccaaca ggcttcgagg
     3001 cactggctac ggcttgcctc gcggcccttc ctccccgtag attgggagga tgagcgaaac
     3061 ctccacccca gccgcggggc gccaccaggg ccccgccctc cggtccgccc tccgggccgc
     3121 cctgtgcctc gccctcctcg gcgcgggagc cctcggacca gccctcgccg aagagtcccc
     3181 ccagcagccg cagcccgccc aggcgcctca gaccgggctc gtcccggact ccttcccctt
     3241 ccgcctcagc gcggtcaagg aggccgcgat aggcgccggg ggcctgggca tctacggctc
     3301 gagcctctat ctcgaggcgc ggaagccctc gcccagggcc tccgacatca acgcctccga
     3361 catccccttc ttcgaccgcc tctacgtcac aagccatccg gcctggatgg gcacggccgc
     3421 cgacgccctc atggtcacgg ctgccgtcgt gcccgagctc gcggcggccg ccttcgtgcc
     3481 cgacttcagc ctcgggaagt tcgccaccat cggcgtcatg tacgccgaga ccatgggcct
     3541 cgcctatgcc acgcccaacc tgctcaaggc ggcgatcaca aggtacaggc cctacgccta
     3601 tgcgagcacg gtcgacctca ccgactccga gctgaactcg tccttcccct cgcggcatgc
     3661 ggtgatcgcc ttctcctcgg ccgtcttcgc gggctatgtc ttcgacgagc tagccccgga
     3721 ctcgccatgg aggaccctgg tctgggtctc tggcctcggc ctggcgacgg ccacctcggc
     3781 cttgagggtc gcctcgggag accatttcct gagcgacgtg gtggccggcg gagtcttcgg
     3841 cgccgccatc ggcttcgccg tgccctggat gcaccgcacg aggagcacgg gcgagggtct
     3901 gtcgctcgcc cccctgcccg gcggcctcct cgtcagcata gggctctgac cccccagccg
     3961 ggctagcgcc cgagccaggc ctccttgtgg gccgtgaacc aggcgaagag ggcggcagcg
     4021 cggttgacga aggactcctg gagcctgctc cggaagatac cgggagcctt ggctccgctc
     4081 gccgtgtaga agacgacagc gtccgaggcc tggaccacga agagccaggt cttgagcgcc
     4141 ccgggcgcgg cgatgggcac tatgccccag ctcatcttcg tgacgttgcc cacctccacc
     4201 atgacagccc cttgaagtcc ccggaaagcg tagctgtaga cgttctgccc gaagctgagg
     4261 tcccgctgga aggcgaagag gctctcggcc tcggggaggg ccgaggccga gggggaaggg
     4321 gggtcgggca ggcgccgcct ggtctcaggg ccgtcgatcc tgtaggactc ggcgtagagg
     4381 gtcctcatcg agtggtggct gaccgagtag tactggatcc cctcgaggct cgagaaggac
     4441 cgaaggaggc cgtagatggc cgcgagctcc atctttgtgc ggtcggattc ggcaggggcg
     4501 gccctgggca gcaggaacac cgtctcgacc aggatgcccg gtctctcggc ggcgatcgcc
     4561 gccctgacaa ggtcagagcc cgggaaggct ggaatgaggg aggggctgcc cgaatccgag
     4621 gtccgcacgg ccttgccctt cgcgacgaga tcatcgtaga agggagcgca gccggtccgg
     4681 gcggcctcga gcccaggctc tgggggctgc gccgccgccg aggcgcacag gaagaaaagg
     4741 aggaggccga ggccaaggct ctgccggatg agcgagtgcg gtcgttcctg catggtgggc
     4801 cgatgatagc ccgcgggggg tggccccgcg caagtgggag cggctccgga tgggggccgg
     4861 ccggatctgg cgcggccccc ttccagcggc ctcagggcag gttggagttg aagcagtcgc
     4921 ggaatatctt ccaggaaccg tcagcctgct tccggaagac tgtcaggaac tttccgtcgt
     4981 agctgagggg gccggagtcc tttggtctcg cggaccagct gtagactccg cgggcgaagg
     5041 ccaggcctcc ctccaccttg acctcctggg tgtcgatctt catcgagaat gcgttcgcgg
     5101 gccggctcgg gattcggacg acggcgaggt tgcggatctc ggccttgccc atatgcatgc
     5161 cctcgttggg gggaagctga accccgtcct cgtcccagag ggccagccag ccttcggcgt
     5221 ctcccgccat gagggtactg gagtatcggt cgaagttggc cctgatggca gcctccacag
     5281 ccttctcata ggccggaccg gtagagaggg gtcttggctt gagacccagc tggccaagga
     5341 tggtcatgct gttgaggtac cacttgagtg aggtagcgag gccatcgctt ccgtagtggc
     5401 cgaggtaggc cgtcctcacc gagatgggcc tgcctgtggc ctggttgccg agccagctgc
     5461 ccgtgttgct gccggagagg accagctcgc tcacgaccca gcccccgccc aggtcgatgc
     5521 tgcgcacggg cttgaaggca aggtcgggga aggccgagat caaggttccc atccaagcga
     5581 catacgaatc ccttcccacc tcttcgaaga gcggagagac aaggaccttg gcgctccggg
     5641 cgagcatctg gccgagggag cccatgtcgt gcttgccgag cctctccagg gtctccagat
     5701 gggctgccat cggcttgagc cccgtgggcc tggggtcctc gggccagcca gccgaaccgg
     5761 gggcgggggg ctggggcaag gtcgcctcga tgcgcccaag ggcgtagtcc tcggccgccc
     5821 cctcgccaaa ggtggtcatc accttcatct tgccgttttc gaagctcacg atgtccgcat
     5881 ggaagacgcg gaagggcaaa ccggtgagcg gattggtccg aacgaatgag cactcatcga
     5941 agaaggcgag atctcccgag acgaggatct gtttctggaa atggtagagt ttgggatcgg
     6001 cggcgagcct ccccccaatc ctcgcgctga aatccgcccg cgagaccggt tttggagaag
     6061 cgaccgcgtc ctggacgaaa tcccccgtcg tgaaggtcat gtacttggcg atatcgtggc
     6121 cggtcaacgc gaggtcgctc gcctcgatga tcgagaccag atcgtatcgc tgctgcgcgg
     6181 cctgcggccg caggggcagt gtggcgagga tcgtgcacag gactaggggg atgagggaga
     6241 taaaagcttt cttcacggcg atctcctttt gaccggcggc accggcccat ggacggattt
     6301 cctccatcat caaggcctct cgttgcaaaa acgttgcacg gtctcggacg cgggcatata
     6361 atcccgctca gttaatcaat cctcggttgg tggtcccatg ctagggattg tccgcatcag
     6421 gcttctcggt cctcccttcc tcgagacctc agctcgacgc attgccttgt cccgaaggaa
     6481 atcccttgcc ctgctcgcgt ggatcgccgt caaggggccg cagcggaggg acagcgcggc
     6541 cgccctcctg tggcccgaga gcggcggggc gagcgcgcgc accaacctcc gctccgcgat
     6601 cttcgatatc aacacggcct cggacgccga gatcctggcc ctttcggacg actcgatagg
     6661 cttggtgcca gaccgagtcg atcttgacca cgcggccttc gaggcggcga tggcatcctg
     6721 cccccgctgc cgccgctccg cctgctgcgg c
//
LOCUS       DASSQV010000173         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_914313, whole genome shotgun sequence.
ACCESSION   DASSQV010000173 DASSQV010000000
VERSION     DASSQV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914313"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..966)
                     /locus_tag="VFL04_03020"
     CDS             complement(91..966)
                     /locus_tag="VFL04_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET7838706.1"
                     /translation="MPGRDERSINSALAAIHARLESLSESERKVADYVIAQPKKTLHL
                     NVAELAKQCGASQASVVRFCKRLGLGSYGDFKLRLARDVFQENDERFMPDLDLESGAS
                     AEEAIENVVAMTERGLALLGSTLDPRAVEAAADRILSSSMTALFGVGASGIVAYDFLQ
                     KLLRIGLPASFAMDSDLQVTTASSLRPTDAAFVVSYSGENLAMIEVARQAKARGACLV
                     SLTMDGKTSLSSLADIRLAVPSSERIYRKAAVTSRINQLTVVDILYSIIVSRNLDGSI
                     AAIERTMRATHRGKA"
     gene            complement(950..1903)
                     /gene="murQ"
                     /locus_tag="VFL04_03025"
     CDS             complement(950..1903)
                     /gene="murQ"
                     /locus_tag="VFL04_03025"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HET7838707.1"
                     /translation="MIDLSITQDMLESFTTEGRNPSSMDIDSKSTIDILRIINEEDRK
                     VPLAVEKALEPIAALVDDVVAAFRAGGRLVYIGAGTSGRLGVLDASECPPTYGVPPTM
                     VQGIIAGGHKALVSSIEGAEDDFEAGAKELEMIGFSGRDVLVGITASGQAPYVLGAMS
                     RAQELGAVVAAISCNRGSKTFQHAKHVVYLDVGPEVVTGSTRMKAGTAQKLVLNMLST
                     ASMIRYGKVYHNLMVDLAPVNKKLVERAKRLVCQATGCTREEAEAAFEASGKRPKTAI
                     IMVLLKVGRQEAERLEAVGGGRISDAVAVHAAEGKGDAGQG"
     gene            complement(1900..>2905)
                     /locus_tag="VFL04_03030"
     CDS             complement(1900..>2905)
                     /locus_tag="VFL04_03030"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HET7838708.1"
                     /translation="EPRWLELAAHGAAFIRDKGPSPSGDFYFALDRSGRPLVQPYNIF
                     SDFFCAAGFAEYGKASGEAWATELAARTWRRILERRSRPKGVWTKQIAENRPVKAMGM
                     SMMLLWMAEVMQGVAPREELEAIVSQSLDQIFSIHVDPGRRAVFERVFADGTRPDCME
                     GRLLNPGHALETLWFAMRAAERRADLELIERSREAMLWTIERGWDKSYGGIYYYQDYE
                     GFPPEKLESDMKLWWVHVEALCAFLLAYKLTGDPVCEAWFHRIHDWTWSHFPDREHGE
                     WYGYLDRRGEVAQSLKGGKWKGFFHLPRALMECSAWLAQMEASCAGDSYNAKQAGGGA
                     "
BASE COUNT          441 a          980 c          998 g          486 t
ORIGIN      
        1 cctcccagag acaggcgttg gcagaatcgg cgatgcggag ccgggccgag ctccctcccc
       61 cgtcgacgcc gaagaccgga gcgcccagca tcaggccttg ccgcggtggg tggcgcgcat
      121 cgtgcgctcg atggccgcga tcgagccgtc gaggttgcgc gagacgatga tcgaatagag
      181 tatgtcgacc acggtgagct ggttgatccg cgacgtgacc gcggccttgc ggtagatcct
      241 ctccgatgag ggcacggcga ggcggatgtc ggcgagggag ctcaagctgg tcttgccgtc
      301 catcgtgagc gagacgaggc aggcgcccct ggccttcgcc tgtctggcca cctcgatcat
      361 ggccaggttc tcgcccgagt acgagacgac gaaggcggcg tcggtcggcc taagcgacga
      421 ggccgtcgtg acctggaggt cggagtccat cgcgaaggag gcgggcaggc cgatgcgcag
      481 gagcttctgg aggaaatcgt aggcgacgat ccccgaggcc cccacgccga agagggccgt
      541 catcgacgac gagaggatgc ggtcggcggc cgcctccacc gccctcggat cgagggtcga
      601 gcccaggagg gcgaggccgc gctccgtcat cgccaccacg ttctcgatgg cctcctcggc
      661 gctcgccccg gactcgaggt cgaggtcggg catgaagcgc tcgtcgttct cctggaagac
      721 gtcccgcgcg aggcggagct tgaagtcccc atagctgccg aggccgaggc gcttgcagaa
      781 gcgcacgacc gaggcctggc tcgccccgca ctgcttcgcc agctcggcca cgttgaggtg
      841 gagggtcttc ttcggctggg ctatcacgta gtcggctacc ttgcgctccg actcggagag
      901 cgactcgagc cgcgcgtgga tcgcggccag ggcgctgttt atgctgcgct catccctgcc
      961 cggcatcgcc cttcccttcg gccgcgtgga ccgcgacagc gtcggagatt cggccgccgc
     1021 cgacggcctc caggcgctcg gcctcctgtc ggccgacctt gaggaggacc atgatgatgg
     1081 cggtcttcgg ccttttcccc gaggcctcga aggcggcctc ggcctcctcg cgcgtgcagc
     1141 ccgtggcctg gcagaccaga cgcttggcgc gctcgacgag cttcttgttg accggggcga
     1201 ggtcgaccat gaggttgtgg tagaccttgc cgtagcggat catgctggcc gtgctcagca
     1261 tgttgagcac gagcttctgg gccgtgcccg ccttcatcct cgtcgagccc gtcacgacct
     1321 cggggccgac gtcgaggtag accacgtgtt tggcgtgctg gaaggtcttg gagccgcggt
     1381 tgcaggatat ggcggcgacc acggctccga gctcctgggc acgcgacatg gcgccgagga
     1441 cgtagggggc ctggcccgag gcggtgatgc ccacgagcac gtcgcggccc gagaagccga
     1501 tcatctccag ctccttggcc cccgcctcga aatcgtcctc ggcgccctcg atcgaggaca
     1561 cgagggcctt gtggcccccg gcgatgatgc cctggaccat ggtcggaggg acgccatagg
     1621 tgggcgggca ctccgaggcg tcgaggaccc cgagcctgcc cgaggtcccc gcccctatgt
     1681 acacgagcct gccccccgcc cggaaggcgg cgacgacgtc gtcgacgagg gcggcgatcg
     1741 gctcaagggc cttctcgacc gcgaggggga ccttcctgtc ctcctcgttg atgatccgca
     1801 ggatgtcgat cgtgctcttc gagtcgatgt ccatcgagct cgggttccgc ccctcggtcg
     1861 tgaagctctc gagcatgtcc tgggtgatgc tcaggtctat catgccccgc ctcccgcttg
     1921 tttcgcattg taggagtccc cggcgcagga ggcctccatc tgggccagcc aggccgagca
     1981 ctccatgagg gccctgggca ggtggaagaa gcccttccac ttcccgccct tgaggctctg
     2041 cgccacctcg ccgcgccgat ccagatagcc gtaccactcg ccgtgctcgc ggtcggggaa
     2101 atggctccag gtccagtcgt ggatgcggtg gaaccaggcc tcgcagaccg ggtcccccgt
     2161 gagcttgtag gcgagaagga aggcgcagag cgcctcgacg tggacccacc acagcttcat
     2221 gtcggactcg agcttctccg gcgggaaacc ctcatagtcc tgataatagt atatgccgcc
     2281 gtagctttta tcccaaccgc gctcgatggt ccagagcatg gcctcacgcg agcgctcgat
     2341 gagctcgagg tcggccctcc gctcggccgc ccgcatcgcg aaccagaggg tctcgagggc
     2401 gtggccgggg ttgaggaggc ggccctccat gcagtcgggc ctcgtgccgt cggcgaagac
     2461 ccgctcgaag accgccctcc gacccgggtc gacgtggatc gagaagatct ggtccaggct
     2521 ctgcgagacg atcgcctcga gctcctccct cggggcgacg ccctgcatca cctcggccat
     2581 ccagaggagc atcatcgaca tgcccatggc cttgacgggg cggttctcgg cgatctgctt
     2641 ggtccagacc cccttgggcc tcgatctgcg ctcgaggatc cgcctccagg tcctggccgc
     2701 gagctccgtg gcccaggcct cgcccgaggc cttgccgtat tcggcgaagc cggcggcgca
     2761 gaagaagtcc gagaagatgt tgtagggctg gaccaggggc ctgcccgagc ggtcgagggc
     2821 gaaatagaag tccccgctcg gggaagggcc cttgtcccgt atgaaggctg cgccgtgggc
     2881 cgcgagctcg agccagcgcg gctcg
//
LOCUS       DASSQV010000174         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2407848, whole genome shotgun sequence.
ACCESSION   DASSQV010000174 DASSQV010000000
VERSION     DASSQV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2407848"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1659)
                     /gene="wbaP"
                     /locus_tag="VFL04_03035"
     CDS             complement(217..1659)
                     /gene="wbaP"
                     /locus_tag="VFL04_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HET7838709.1"
                     /translation="MINDEFDSWYRKRYRRTSSALTTSAFILADLFAAMLSFGVGFFL
                     VNLYDHHAINFRSFVQYWPYLPGFVFLFWMTHLYPGLSMAPAEEMRRFSTGSFLAHAG
                     LILSLYVQHANKGSVSFAFATSFLISLVGFPTCRAIARDLLSHMRWWGIPAVVFGAGD
                     MGREVVDSLLDEKRLGYRPAAILDDDPALQGEYRGVPILCGTDLGPELVRRYHIRMAI
                     VAMPSVERARVASIVERSVLCFRYYILIPDLLGLMNIWMTVRDFNGLLGLGSSQRLLM
                     PWNLMVKRFLDLAFTIMGGILISPIILLIGILIKLDSSGPVFYGHTRLGKNGKPFKAW
                     KFRSMVKDAEARLREQLAADPELRAEWESNFKLKNDPRITRVGRLLRRTSLDELPQIW
                     NVIRGEMSLVGPRPIVEAEVPFYGSLYERFSSMMPGMTGLWQVSGRSDTNYDERVAFD
                     RFYNESWSVWLDLHILFRTFQVVFRGKGAY"
     gene            complement(1706..>2462)
                     /locus_tag="VFL04_03040"
     CDS             complement(1706..>2462)
                     /locus_tag="VFL04_03040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7838710.1"
                     /translation="GGLKRAVYLWLEVFLSGMTDIILFQNRHELAQAEAHGMGRRASL
                     RYLGNGIDFADFPAPRESRRKPKAFRLLCVARVEPKKNHLMLVRAVELLRRRYGHSNL
                     ELFCVGEVNDGSAPEEAKRLGLEAAIHFTGVKTKDEVAAYLSSCHISVLSSVAEGLPR
                     GLMESMLFGLPCVATDVTGSNEVVVDGENGFLVPLGDHEAMAERIDRIIREDGLYERL
                     SRRALEQALSRFDEDRVIGDLKAIYREAARARR"
BASE COUNT          431 a          761 c          874 g          396 t
ORIGIN      
        1 gcctcgaggg ccctgcgcag ggcctggccc accgggcccg aggggaggag ccgcagatcc
       61 ccttcggtcg agcccgtgct tgaggagccc tcggtccgca tggccgccgc gagggccgag
      121 gggccaagct cggggtagag ctcggcgaac ttcctcaagc cctgtgctgg tgcggcggca
      181 agggcggcgc aggaaaggaa cgcgagggcg agggccctag tacgcgccct tgccacggaa
      241 gaccacctgg aaggtgcgga agaggatgtg caggtcgagc cagaccgacc agctctcgtt
      301 gtagaagcgg tcgaaggcga cgcgctcgtc gtagttggtg tccgagcggc ccgagacctg
      361 ccagagcccc gtcatgccgg gcatcatgct cgagaacctc tcgtacaagc tgccgtagaa
      421 ggggacctcg gcctccacga tggggcgcgg tccaacgagg ctcatctccc cgcggatgac
      481 gttccagatc tgcggcagct cgtcgaggct ggtgcgtctg aggagcctgc ccacgcgggt
      541 gatccgcgga tcgttcttga gcttgaagtt gctctcccac tcggcgcgga gctcggggtc
      601 ggccgccagc tgttcgcgca gcctcgcctc ggcgtccttc accatcgacc tgaacttcca
      661 ggccttgaag ggcttgccgt tcttgccaag gcgggtgtgg ccgtagaaca cgggtcccga
      721 cgagtcgagc tttatgagga tgccgatgag gaggatgatc ggcgagatga ggatcccgcc
      781 catgatcgtg aaggcaaggt cgaggaagcg cttgaccatc aagttccacg gcatgaggag
      841 gcgctggctt gatcccaggc cgagcaggcc gttgaagtcc cttacggtca tccagatgtt
      901 catgagcccg aggaggtcgg ggatgaggat gtagtacctg aagcagagga cgctccgctc
      961 gacgatggag gcgaccctgg ccctctccac gctcggcatc gccacgatcg ccatcctgat
     1021 gtggtagcgc ctgacgagct ccgggccgag gtcggtgccg cagaggatgg gcacgccccg
     1081 gtattcgccc tggagggcgg gatcgtcgtc caggatcgcc gcgggcctgt agccgagccg
     1141 tttctcgtcc aggaggctgt ccacgacctc gcggcccata tcgccggcac cgaagacgac
     1201 ggcggggatc ccccaccagc gcatgtgtga gaggaggtcc cgggcgatgg ctcggcaggt
     1261 cgggaagccg acaaggctga tgaggaagct cgtcgcgaag gcgaaggaca ccgagccctt
     1321 gttggcatgc tggacgtaga gcgagaggat gaggccggcg tgggcgagga aggagccggt
     1381 ggagaagcgc cgcatctcct ccgcgggggc catgctcagg ccggggtaga ggtgggtcat
     1441 ccagaacagg aacacgaagc ccgggaggta gggccagtac tgcacgaagg acctgaagtt
     1501 gatggcgtgg tggtcgtaga ggttcaccag gaagaatccc acgccgaagc tcagcatggc
     1561 agcgaagagg tcggcgagga tgaaggccga ggtcgtcaac gccgagctcg tgcggcggta
     1621 gcgcttcctg taccaggagt cgaattcatc attgatcatg ttcggtccag tatccatccg
     1681 atccgccttt cccgcaagca ggacctcagc gcctcgcccg ggccgcctcg cggtagatcg
     1741 ccttgagatc gcctatcacg cggtcctcgt cgaagcggga gagggcctgc tcgagggcgc
     1801 gccgggagag ccgctcgtac agcccatcct cgcggatgat cctgtcgatt cgctcggcca
     1861 tggcctcgtg gtcgccgagg ggcacgagga agccgttctc gccgtccacc accacctcgt
     1921 tgctgcccgt gacgtcggtc gcgacgcagg gcaggccgaa gagcatggac tccatgaggc
     1981 cgcggggcag gccctcggcc accgaggaca ggacggagat gtggcaggag gagaggtagg
     2041 cggccacctc gtccttggtc ttcactccgg tgaagtggat ggcggcctcg aggccgagcc
     2101 tcttggcctc ctcgggggct gagccgtcgt tgacctcgcc gacgcagaag agctcaagat
     2161 ttgagtggcc gtatcgccgc cgcaggagct cgaccgcccg cacgagcatg aggtggttct
     2221 tcttgggctc gaccctcgcg acgcagagga gcctgaaggc cttgggcttg cgccggctct
     2281 cgcgcggggc cgggaaatcg gcgaagtcga tgccgttgcc aaggtacctc aggctggccc
     2341 tcctccccat gccgtgggcc tcggcctggg ccagctcgtg cctgttctgg aagaggatga
     2401 tgtccgtcat ccccgagagg aagacctcga gccagaggta caccgcccgc ttgagccccc
     2461 ca
//
LOCUS       DASSQV010000175         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_915521, whole genome shotgun sequence.
ACCESSION   DASSQV010000175 DASSQV010000000
VERSION     DASSQV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915521"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFL04_03045"
     CDS             <1..184
                     /locus_tag="VFL04_03045"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838711.1"
                     /translation="VAPLPLRALELERFLVGREADEETAALAGDVAVRGAFPLARNAY
                     KLQVMKGLVRKAILDL"
     gene            complement(181..1353)
                     /locus_tag="VFL04_03050"
     CDS             complement(181..1353)
                     /locus_tag="VFL04_03050"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HET7838712.1"
                     /translation="MVDDIRRLSSELRGAMIGFASDLVRIKSFTGREEAIIGRIKAEM
                     ESLGYDDARIDGMGNLVGRIGSGKRSILFDSHVDTVAVTDAGRWTREPFGGELVEGML
                     YGRGSVDMKSAVAASVYAGAAAKRLWPGLDRTVLISTSVMEEDYDGESLVYEFGPGGL
                     RPDAVVICEPSDCTISLGQKGRALLRIDMPGVSAHGSAPEKGDNPVYRMNTVIRRVEE
                     LGLRLMGEGGASGSIALTGISCETASLNAIPATCTAFVDRRLVRGQDEASIASEMTGL
                     VAGTPAIWEVQKVVGKSWTGREVVLDSFLPAWELEASHPLAQAAVSAWEGLFPGKARL
                     STWDFSTNGVASARLGIPTIGFGPGDSKQAHRVDEHCPVEQIVRAFEFYLALAARF"
     gene            complement(1424..1705)
                     /locus_tag="VFL04_03055"
     CDS             complement(1424..1705)
                     /locus_tag="VFL04_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838713.1"
                     /translation="MSTLKMSLSFENFAISAWDRAGSPALRIVLLPGELISVPRKESM
                     LRVVSGRAWASYDGKDYDLGPGATLELETRSDSALIQAMEAEALVFEVR"
     gene            1823..>2602
                     /locus_tag="VFL04_03060"
     CDS             1823..>2602
                     /locus_tag="VFL04_03060"
                     /inference="COORDINATES: protein motif:HMM:NF002964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HET7838714.1"
                     /translation="MLDYRLIEAFAAVIEEGGFERAANRLRVTQSAISQRIKQLEDEV
                     GRILVLRESPPRATEAGERLIRHYRQVASLEEEAIEELGMSGRGGYQHLPIAVNADCL
                     SVWLLEAILPYLRRTAVTLEIMVDDQDRTLRFLRSGEAAGCVSAQKLSIQGFTSTQIG
                     ELRYLLCASPGFARQWFPEGIDREAASRAPCIHFTRFDQLQYRALERLFGRPRIEPPA
                     FYIPSTEKFIDAAVAGLAYAMIPEVQAAPLIREGALIELDPG"
BASE COUNT          416 a          891 c          880 g          415 t
ORIGIN      
        1 tgtggccccc ctcccccttc gcgccctcga gctcgagcgc ttcctcgtcg gcagggaggc
       61 ggacgaggag acggccgcgc tggcggggga cgtggcagta cggggagcct tcccccttgc
      121 gcgcaacgcc tacaagcttc aggtcatgaa gggcctcgtg cgcaaggcca tcctcgatct
      181 ttagaagcga gcggcgaggg cgaggtagaa ctcgaaggcc ctgacgatct gctcgacggg
      241 gcagtgctcg tcgacgcggt gggcctgttt tgagtccccg ggtccaaagc cgatcgtggg
      301 tatgccgagc cgcgcgctcg cgaccccgtt ggtgctgaag tcccaggtgg agagcctggc
      361 cttcccgggg aagagcccct cccaggccga gacggccgcc tgggcgaggg gatgggaggc
      421 ctcgagctcc catgctggga ggaaggagtc gaggacgacc tccctgcctg tccagctctt
      481 cccgacgacc ttctggacct cccagatcgc cggggtcccg gcgacgaggc cggtcatctc
      541 ggacgctatc gaggcctcgt cctggcccct gaccagcctg cggtcgacga aggccgtgca
      601 ggtggcgggg atcgcgttga gcgaggccgt ctcgcaggag atgccggtga gcgcgatcga
      661 gcccgaggcg cccccctctc ccatgagtcg gaggccgagc tcctcgaccc tgcggatgac
      721 cgtattcatc ctgtacaccg ggttgtcccc cttctcgggg gctgaaccgt gggccgagac
      781 ccccggcatg tcgatgcgca gcagggccct gcccttctgg ccgagggaga tcgtgcagtc
      841 gctcggctcg cagatcacga cggcgtcggg cctgaggcct cccggcccga actcgtagac
      901 cagggactcg ccgtcgtagt cctcctccat caccgaggtg gagatgagga ctgtccggtc
      961 caagccgggc cagagccgtt tggcggcggc cccggcatag acgctggcgg cgaccgcgct
     1021 cttcatgtcg accgagccgc ggccatagag catgccctcg accagctcgc cgccgaaggg
     1081 ctccctggtc cagcgcccgg cgtcggtcac ggcgaccgtg tccacgtggg agtcgaaaag
     1141 gatgctccgc ttccccgagc cgatccgccc cacgaggttg cccatgccgt cgatccgggc
     1201 atcgtcgtag ccgaggctct ccatctcggc cttgatccgg ccgatgatgg cctcctcgcg
     1261 gcccgtgaaa ctcttgatgc ggacgaggtc ggaggcgaag ccgatcatgg ccccgcgcag
     1321 ctcggaggag aggcgccgta tgtcgtctac catggggtgc gtgtaggccg ggccggcccg
     1381 gagcggaagg gccggccccg ggctgggggg ccggccgcgg agcctaacgg acctcgaaga
     1441 cgagggcctc ggcctccatc gcctggatga gggccgaatc tgagcgggtc tcgagctcga
     1501 gggtggcgcc gggaccgagg tcatagtcct tcccgtcgta ggaggcccag gcccggccgg
     1561 agacgacgcg gagcatgctc tccttcctgg ggacgctgat gagctcgccc gggaggagga
     1621 cgatccggag ggccggcgag cccgccctgt cccatgccga aatggcgaag ttctcgaagc
     1681 tcaggctcat cttcagggtg ctcatgtcag tcctccttcc cagggtgcag atcctgcccg
     1741 ggtacgagga gaacatagca gaacgcgaaa tattagtgaa atgaataatt ctaatatgtc
     1801 atgaattgtg ctaatatgag gaatgctgga ttatcgcctc atcgaggcct tcgccgccgt
     1861 catcgaagag ggcggcttcg agcgggccgc caacaggctt cgagtcaccc aatccgcgat
     1921 ctcgcagcgg atcaagcagc tcgaggacga ggtgggccgc atcctcgtgc tcagagagag
     1981 cccgccccgg gccaccgagg ccggggagcg cctcatccgc cattaccgcc aggtcgccag
     2041 cctcgaggag gaggcgatcg aggagctggg catgagcggc cggggcggct accagcacct
     2101 gccgatcgcc gtcaacgccg actgcctctc ggtctggctc ctcgaggcga tcctgcccta
     2161 cctcagacgg acggcggtca ccctcgagat catggtggac gaccaggaca ggaccctgcg
     2221 tttcctccgc tctggcgagg ccgcgggctg cgtcagcgcc cagaagctct ccatccaggg
     2281 cttcacctcg acccagatcg gggagctccg ctacctcctc tgcgcctcgc ccggcttcgc
     2341 ccggcaatgg ttccctgagg gcatcgacag ggaagccgcg tcgagggccc cctgcatcca
     2401 cttcacccgt ttcgaccagc tccagtaccg ggccctcgag cggctcttcg gcaggccgag
     2461 gatcgagccc ccggccttct acataccctc gaccgagaag ttcatcgacg cggccgtggc
     2521 cggcctcgcc tacgcgatga tccccgaggt ccaggccgcg cccctgatca gggagggcgc
     2581 cctcatcgag ctggatcccg gg
//
LOCUS       DASSQV010000176         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_44650, whole genome shotgun sequence.
ACCESSION   DASSQV010000176 DASSQV010000000
VERSION     DASSQV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44650"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VFL04_03065"
     CDS             <1..698
                     /locus_tag="VFL04_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7838715.1"
                     /translation="KPRELTQKDIDKAVEYSLEQRPAPPSNASLAFAAVREAVVRIRQ
                     LQGEGAKEQEIGVGTGVVVNEEGLIITNNHVIAGAPRLDVTFSDGSESPAQVVSADPD
                     RDLALVKADQSPDELKPATLRSTAGLKVGDEVFAVGFPFGIGPSLSAGVVSGLDRSYV
                     SPARGKVLTGLIQVDAAANPGNSGGPLVDRQGDVVGLVTSILNPSEQRVFIGIAFAVP
                     FDQAMRGFGLNPF"
     gene            708..1790
                     /locus_tag="VFL04_03070"
     CDS             708..1790
                     /locus_tag="VFL04_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7838716.1"
                     /translation="MEASNGEGRPAANASGGAGGNGNGNGSGNGAARAELMEKVLYEV
                     KKIVVGQDHFLERVLVAILANGHLLVEGVPGLAKTLTIKTLAESVQGSYRRIQFTPDL
                     VPADLVGTRIYNQKTGEFSTSPGPVFTNLLLADEINRAPAKVQSALLEVMQEYQVTIA
                     GKSYKVPRPFLVMATQNPIETEGTYPLPEAQVDRFMMKVIVGYPSEDEEFVIVERITG
                     IPEAVGAVASTEQLLALQARTRKVYVDPSLMRYAVKLSSASRDPGRAGLGELSRYILY
                     GASPRATIHLVEASKALALLRGRDYVHPQDLIDLVPDVLRHRLVLSYEAVVDSVSSDE
                     ILARIMEWIPAPEAPMESHVELRADT"
     gene            1765..2697
                     /locus_tag="VFL04_03075"
     CDS             1765..2697
                     /locus_tag="VFL04_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7838717.1"
                     /translation="MSSSAPIPEALLRRLEWTVLRRLDGLLQGDYRSLFRGLGMDLAE
                     IREYQYGDDVRFMDWNVTARLGSPYVRRFDEDRDLTAWFVLDLSASVHFGSGQATKLE
                     LLAGFTGTIARLLSGKGNRVGALIFDGRDQELVSPKGGRLHLLYLLDRILSRPPIGPS
                     PPTDLRAPLMAAVDLAKRRSLVFVVSDFFSPPGWAEPLSYLARRHELVAVRLIDPTET
                     ELPDLGIVTLQDSETGEQIQVNSHSRALRARFAEASARREEALRAAFDRANVDVLELS
                     TRDDLALAILGFAELRKRRSQLAGAATVTRRAAS"
     gene            2694..>3515
                     /locus_tag="VFL04_03080"
     CDS             2694..>3515
                     /locus_tag="VFL04_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7838718.1"
                     /translation="MSFLWPGMLWLLLVVPLLVAAYIFLLRRRSRSAVRYPAMGLLRE
                     AMGKGPDLRRHLPPLLVLLALAATIVATARPQGEVTLPSDSGTVILAMDISGSMRARD
                     IEPSRIQASQAAARAFVKAQPSSVKVGVVAFAATATLVQPPTQDRDAVLAAIDRFRLQ
                     RGTAVGNGILASLQAIFENSNLDLGPIDPSARSSPLDPNAPPPPPPPEPVEPGSYRSA
                     AIILLTDGQTNTGADPIESAHKAADLGVRVFTVGLGTTGGQIVGFGGWSMRAQLDE"
BASE COUNT          541 a         1348 c         1128 g          498 t
ORIGIN      
        1 agaagccccg ggagctcacc caaaaggaca tcgacaaggc ggtggagtac agcctggagc
       61 agcgccccgc cccgccctcg aacgcctcgc ttgccttcgc ggccgtcagg gaggcagtgg
      121 tgaggatcag acagctccag ggcgaggggg ccaaggagca ggagatcggc gtgggcacgg
      181 gggtcgtggt gaacgaggag ggactcatca tcacgaacaa ccacgtgatc gcgggggcgc
      241 cccggctcga cgtgaccttc tccgacggga gcgaatcccc ggcccaggtg gtatcggccg
      301 accccgaccg cgacctcgcc ctcgtgaagg ccgaccagag ccccgacgag ctcaagcccg
      361 ccaccctgcg cagcacggcc ggcctcaagg tcggcgacga ggtcttcgcc gtgggcttcc
      421 ccttcggcat cgggccctcg ctctcggcgg gggtcgtgtc gggcctcgac cgctcctacg
      481 tctcgcccgc ccggggcaag gtcctcacgg gcctcatcca ggtcgacgcg gccgccaacc
      541 ccggcaactc gggcgggccc ctcgtggacc gccagggcga cgtcgtgggc ctcgtcacct
      601 cgatcctgaa cccctcggag cagcgggtgt tcataggcat tgccttcgcc gtgcccttcg
      661 accaggcgat gcggggcttc ggcctcaacc cattctagga gaacggcatg gaagcctcga
      721 acggagaagg aaggccggcc gccaacgcca gcggcggcgc aggcggcaac gggaacggaa
      781 acgggagcgg gaacggagcc gcgcgggccg agctcatgga gaaggtcctc tatgaggtga
      841 agaagatcgt ggtgggccag gaccacttcc tcgagcgggt cctcgtggcc atcctcgcga
      901 acggccacct cctcgtcgag ggcgtgcccg gccttgccaa gaccctgacc atcaagaccc
      961 tggccgagag cgtccagggc tcctaccggc ggatccagtt cacccccgac ctcgtgcccg
     1021 ccgacctcgt gggcacgagg atctacaacc agaagaccgg ggagttcagc acctcccccg
     1081 gtcccgtgtt caccaacctc ctcctggccg acgagatcaa ccgcgccccc gccaaggtcc
     1141 agagcgccct gctggaggtg atgcaggagt accaggtcac catcgccggc aagagctaca
     1201 aggtgccccg gcccttcctc gtcatggcca cccagaaccc catcgagacc gagggcacct
     1261 atcccctgcc cgaggcccag gtggaccgct tcatgatgaa ggtcatcgtg ggctatccga
     1321 gcgaggacga ggagttcgtg atcgtcgagc gcatcaccgg gatccccgag gcggtgggcg
     1381 ccgtggcgag cacggagcag ctcctggccc tccaggccag gacgaggaag gtctacgtcg
     1441 acccctccct catgcgctac gcggtcaagc tctcgagcgc cagccgcgac ccggggcggg
     1501 ccggcctcgg cgagctgtcc cgctacatcc tctatggggc gagcccccgc gcgacgatcc
     1561 acctggtcga ggcctccaag gccctcgccc tcctgcgcgg cagggactac gtccaccccc
     1621 aggacctcat cgacctcgtg cccgacgtgc tcaggcaccg cctcgtcctg tcctacgagg
     1681 ccgtcgtcga ctcggtgagc tccgacgaga tcctggccag gatcatggag tggataccgg
     1741 cgcccgaggc gcccatggag agccatgtcg agctccgcgc cgatacctga ggccctcctg
     1801 cgccgcctcg agtggaccgt cctgcgcagg ctcgacggcc tcctccaggg cgactaccgg
     1861 agccttttcc gcggcctcgg gatggacctc gccgagatca gggaatacca gtacggggac
     1921 gacgtccgct tcatggactg gaacgtcacc gcgaggcttg gctcgcccta tgtgaggagg
     1981 ttcgacgagg acagggacct cacggcctgg ttcgtcctcg acctgagcgc ctcggtccac
     2041 ttcggctcgg gccaggccac gaagctcgag ctcctcgcgg gcttcacggg cacgatagcc
     2101 cgcctcctct cgggcaaggg caacagggtg ggcgccctca tcttcgacgg cagggaccag
     2161 gagctcgtga gccccaaggg gggacggctc cacctgctct acctcctcga ccgcatcctc
     2221 tcccggccgc caatcggccc ctcgcccccg actgacctcc gggcgcccct catggcggcg
     2281 gtggacctgg ccaagcggcg ctccctcgtc ttcgtggtct ccgatttctt cagccccccc
     2341 ggctgggccg agcccctctc ctatctcgcg cggaggcacg agctcgtggc cgtgcgcctc
     2401 atcgacccca ccgagaccga gctgcccgac ctcgggatcg tcaccctcca ggactcagag
     2461 acgggggagc agatccaggt caacagccac tcccgggccc tgcgcgcccg cttcgccgag
     2521 gcctcggccc ggcgcgagga ggccctgcgc gcggccttcg accgggccaa cgtcgacgtc
     2581 ctcgagctct cgacccggga cgacctggcc ctggccatcc tcggcttcgc cgaactgcgg
     2641 aaacgccgct cccagctcgc tggcgcggcc accgtgacaa ggagggcggc gtcatgagct
     2701 tcctctggcc aggcatgctc tggctcctcc tggtcgtccc cctcctggtc gccgcctaca
     2761 ttttcctgct taggcgcagg agccgctcgg ccgtgcgtta cccggccatg ggcctcctcc
     2821 gcgaggccat gggcaagggt cccgacctga ggcgccacct gccccccctc ctcgtcctcc
     2881 tcgccctcgc cgccacgatc gtcgccaccg ccaggcccca gggcgaggtc accctgccct
     2941 cggactcggg gacagtcatc ctcgccatgg acatctcggg gagcatgagg gcgcgggaca
     3001 tcgagccctc gcgcatccag gcctcccagg ccgcggcgag ggccttcgtg aaggcccagc
     3061 cctcgagcgt gaaggtgggc gtcgtggctt tcgccgccac cgccaccctc gtccagcccc
     3121 cgacccagga ccgggacgcc gtcctcgccg cgatcgaccg cttcaggctc cagcggggca
     3181 cggccgtggg gaacggcatc ctcgcctccc tccaggccat cttcgagaac tccaacctcg
     3241 acctcgggcc catcgacccc tcggcccgga gctcccccct cgacccgaac gccccgccgc
     3301 ccccgccccc gcccgagccc gtggagccgg gctcctaccg gtcggcggcc atcatcctgt
     3361 tgaccgacgg ccagaccaac acgggggccg acccgatcga gtcggcgcac aaggcggccg
     3421 acctcggggt gcgcgtcttc accgtgggcc tcggcacgac cgggggccag atcgtgggct
     3481 tcggcggctg gtccatgcgg gcccagctcg acgag
//
LOCUS       DASSQV010000177         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_542402, whole genome shotgun sequence.
ACCESSION   DASSQV010000177 DASSQV010000000
VERSION     DASSQV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542402"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFL04_03085"
     CDS             complement(<1..249)
                     /locus_tag="VFL04_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7838719.1"
                     /translation="MRYRSSALILAMALLSASALFGAPGPKVCVFVPGVVSGSPTYEQ
                     LVAGAKRAVDEVPGASLKVVEAGFNQADWLDKLSALAAT"
     gene            371..841
                     /locus_tag="VFL04_03090"
     CDS             371..841
                     /locus_tag="VFL04_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7838720.1"
                     /translation="MGSAESPRTYRIGELAAVFGVTSRTIRYYEELGLLEAFEREDGG
                     HRRYDERNAVHLKRIQQLKDYGLSLAEISELFDLARRDRSGESVRKRLAEKYREKLEE
                     AEKRRRALSAYIEDLSWHIEQLERVPDFFDCPGASCASCSFSERCDVRLLLKRA"
     gene            845..>2081
                     /locus_tag="VFL04_03095"
     CDS             845..>2081
                     /locus_tag="VFL04_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET7838721.1"
                     /translation="MPYLSIDIGSSVVKAAYIAEDGRLIGLARRPAPMRHDGQAHEAD
                     ALAWIEGAASASAELAGLYPGTEVRAVSVSGNGPTLLAAGADGLPLGPALSWMDRRAV
                     PEAAEVSRIVEMPVDPSFYLPKALRLWRSSAELRGTVRWFFSCPEYLIYVLSGEAVTY
                     LPHPGYAPYIWDSALLKALELPAERFPPFVAPSRRVGSVASGVAQRFGLKAGTPVVAG
                     FPDFLASIVGSAAVEPGIACDRGGTSEALNLCATRPFPGRELLSLPHAIDGLWNVSAG
                     LSTAGKALEWLASSLGYPGGTGTDGAQDPGATAALLAEASSSAPGARGLLFLPYLAGE
                     RAPLWEPDRRGAFVGLSLSHERGDLARAACESIVYGLRQAAELAKGAGFRFSLVRTSG
                     SPARDGFLNALKADVLGVPV"
BASE COUNT          305 a          773 c          691 g          312 t
ORIGIN      
        1 ggtcgcggcg agggccgaga gcttgtcgag ccagtcggcc tggttgaagc cggcctccac
       61 gaccttgagg gaggcgccgg ggacctcgtc caccgcccgc ttcgccccgg cgacgagctg
      121 ctcataggtc gggctccccg agaccacgcc ggggacgaac acgcacacct tggggcccgg
      181 ggccccgaag agggccgaag ccgaaagcag ggccatggcg aggatcaagg ctgaagacct
      241 gtatctcatg tccgaacacc tcacttctca atgtaacatg gggaacagta tactgaggcc
      301 agagccgcgg cttcgacgcc cgctatgctt acgcggcgcg gaattgacgt gaaaggtaag
      361 gatccatcgt atggggagcg cggaaagccc ccgaacctac cgcatcggcg agctcgccgc
      421 ggtgttcggg gtgacgagcc ggaccatcag atactatgag gagctcggcc tcctcgaggc
      481 cttcgagcgc gaggacggtg gccaccgcag gtacgacgag cgcaacgcgg tccacctcaa
      541 gcggatccag cagctcaagg actacggcct gagcctcgcc gagatcagcg agctcttcga
      601 cctcgccagg cgggaccgct cgggggagag cgtccgcaaa cggctcgcgg agaaataccg
      661 ggagaagctc gaggaggctg agaagcgccg gcgcgcgctc tcggcctaca tcgaggacct
      721 gtcctggcac atcgagcagc tcgagcgggt gcccgacttc ttcgactgcc ccggggcctc
      781 ctgcgcctcc tgcagcttct ccgagcgctg cgacgtccgc ctcctcctga agagggccta
      841 gggcgtgccc tacctctcca tcgatatcgg cagctcggtc gtcaaggcgg cctacatcgc
      901 cgaggacgga cgcctcatcg gcctcgcccg gaggccggcg cccatgcgcc acgacggcca
      961 ggcccacgag gccgacgccc tcgcgtggat cgagggggcg gcttcggcga gcgccgagct
     1021 cgccggcctt tatccaggca ccgaggtccg cgcggtctcg gtcagcggca acgggccgac
     1081 cctcctcgcc gccggggccg acggcctccc cctcggcccc gccctctcgt ggatggacag
     1141 acgcgccgtg cccgaggccg ccgaggtctc acggatcgtc gagatgcccg tcgacccctc
     1201 cttctacctg cccaaggccc tcaggctctg gcgctcctcg gccgagctgc gcgggaccgt
     1261 ccgctggttc ttctcatgcc ccgagtatct gatctacgtg ctgtccgggg aggccgtgac
     1321 ctatctgccc catccgggct acgcccccta catctgggac tcggccctcc tgaaagccct
     1381 cgagcttccg gccgaacgct tccctccctt cgtggcgccg agccgccgcg tcggctcggt
     1441 cgcgtcaggg gtcgcccagc gtttcggcct caaggccggg acgcccgtcg tcgccggctt
     1501 tcccgatttc ctcgcctcga tcgtcggctc ggccgccgtc gagccgggca tcgcctgcga
     1561 ccgcggcggc acatcggagg cgctcaacct ctgcgcgacc agacccttcc ccggcaggga
     1621 gctcctctcc ctgccccacg cgatcgacgg gctctggaat gtctcagccg gcctgtcgac
     1681 cgcgggcaag gcccttgaat ggctcgcctc gagcctgggc tacccaggcg ggacagggac
     1741 ggacggagct caggaccccg gggcgacggc cgccctcctg gctgaggcga gctcctccgc
     1801 cccgggagcc cggggcctcc tcttcctccc ctacctcgcg ggcgaaaggg cccctctctg
     1861 ggagcctgac cggagggggg ccttcgtcgg gctctcgctc tcgcacgagc gcggcgacct
     1921 cgcccgggcg gcctgcgaat cgatcgtcta tggcctccgg caggcggccg agctcgcgaa
     1981 gggagcgggc ttccgcttca gcctcgtgag gacgagcggc tcgcccgccc gcgacggttt
     2041 cctgaacgcg ctgaaggccg atgtcctcgg cgtccccgtc g
//
LOCUS       DASSQV010000178         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2034956, whole genome shotgun sequence.
ACCESSION   DASSQV010000178 DASSQV010000000
VERSION     DASSQV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034956"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            490..1776
                     /locus_tag="VFL04_03100"
     CDS             490..1776
                     /locus_tag="VFL04_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7838722.1"
                     /translation="MAAEFPRLKLDRSLSMFEEAQRLAPGGVMGIRRPYNFVQGEYPI
                     FIDRGYGGHIVDVDGNDYIDMLCGYGPIILGYDEKEINEAVKRQMDERGFCFALVQPV
                     QNALEERLTKLIPCAEQAILVKTGSDATTLAVRIARGYTDRKLILRCGYHGWHDWCVE
                     VRGGVPEEFTDLTLEFEYGKLGELEELLEKHRGQVAGIIVTPVGHPNAHPVVPPPEGY
                     LQGVRDLATKHGAVLIFDEVRTGFRVSMGGAQERYGVTPDLGTFGKAMANGYAISAVA
                     GKREFMKVVEKKVFVSSTFFPNSLEMVAAMKCIDILEREKVPSSIWERGTVFLEKLRK
                     IVADSKVPCSVSGIPPMPYITFEQAPGEAPGTPGEKVYKARREYFYTQTIRRGLFVQP
                     FHHWYIAHRHTDADLQKALTAIAESLELTAKKFPAS"
     gene            1826..2230
                     /locus_tag="VFL04_03105"
     CDS             1826..2230
                     /locus_tag="VFL04_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET7838723.1"
                     /translation="MAESFDEGEFPHIALTERIIKCAIAVHTELGPGLLESIYEEAMT
                     LELQLEGLSFVRQVELPMVYKGHRLAGSYRIDMVVENEVVVELKAATDIHPIHEAQLL
                     TYLRLGGWKVGLLLNFNYRLLRDGVKRIVLSS"
     gene            2302..3126
                     /locus_tag="VFL04_03110"
     CDS             2302..3126
                     /locus_tag="VFL04_03110"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HET7838724.1"
                     /translation="MLDGKIVITCACTGAETTKAQNPALPITAEEIAQTAVEARQAGA
                     AILHLHVRDAEGRPTQKPAVFKKAIELIRAKTDIVVEITTGGAVGDTAEERLAPVTEL
                     EPEMASLDCGTVNFGNDYIVNTLPVMRQFAEEFRRRRVRATLECFDLGHVYSSGILLK
                     EGLMEGPFHYGFVLNVPGAVPFSPANLEAFLAAIPAGANWTVMGIGRASLPAQYAAIA
                     RGGWIRVGFEDNVYYSKGVLAKSNAELVERAARLAREAGLQPASPDDVRKELRLRA"
BASE COUNT          588 a         1084 c          989 g          506 t
ORIGIN      
        1 gacgtgacca tgatcatcgg caacggctac accaaggacc acgccgagat caccctctgg
       61 gagctcaggg agaaccccgg cctcaggaag ctcttcgaga cccagtacgt aaagtaggac
      121 gttcggagcc gatttcggcg cggccccgag ctttaggccg cgaccgaaac cggattccgt
      181 gctataaagg aagcgaggga cggcgagctg gcgcagcaaa accagcgggg gctgtccgtt
      241 cctccgtagg cgatccctcg cctgcgctgg ctaagacaga atgtacgtga ccgcaggcgc
      301 ccgccctcat ggggaggacg gatcgccgcg gtcgcccggg ggctccccgt ccccggcgcg
      361 gcgtgtcctt tgccggcccc tccgggccgg tacccgggag cacgtcggta cagggaggcc
      421 gggccgcgtt tacgcgtcat cccggtgggt ggacgagacc ccccttccct ccacaaaagg
      481 actcacctca tggcggccga atttccccgg ctcaagctcg acaggtccct ctccatgttc
      541 gaggaggccc agcgcctcgc tcccggcggc gtgatgggca tccgcaggcc ctacaacttc
      601 gtgcagggcg agtacccgat cttcatcgac cgcggctacg gcggccacat cgtcgacgtc
      661 gacggcaacg actacatcga catgctctgc ggctatgggc ccatcattct cggctacgac
      721 gagaaggaga tcaacgaggc ggtcaagcgc cagatggacg agcggggctt ctgcttcgcc
      781 ctcgtccagc ccgtgcagaa cgccctcgag gagcggctca ccaagctcat cccctgcgcc
      841 gagcaggcca tcctcgtaaa gacgggctcc gacgcgacca ccctcgccgt gcgcatcgcc
      901 cgcggctaca ccgaccgcaa gctcatcctc cgctgcggct accacggctg gcacgactgg
      961 tgcgtcgagg tgcgcggcgg cgtgcccgag gagttcaccg acctcaccct cgagttcgag
     1021 tacggcaagc tcggcgagct cgaggagctc ctcgagaagc acaggggcca ggtggcgggc
     1081 atcatcgtca cccccgtggg ccaccccaac gcccaccccg tcgtcccccc gccggagggc
     1141 tacctccagg gcgtcaggga cctcgcgacc aagcacgggg ccgtcctcat cttcgacgag
     1201 gtgcgcaccg gcttccgcgt ctccatgggc ggggcccagg agcgctacgg ggtcaccccc
     1261 gacctcggga ccttcggcaa ggccatggcc aacggctacg ccatcagcgc ggtcgccggg
     1321 aagcgcgagt tcatgaaggt cgtcgagaag aaggtcttcg tctcctcgac cttcttcccc
     1381 aacagcctcg agatggtcgc cgccatgaag tgcatcgaca tcctcgagcg cgagaaggtc
     1441 ccctcctcga tctgggagcg gggcacggtc ttcctcgaga agctccggaa gatcgtcgcc
     1501 gactcgaagg tcccctgctc ggtctcgggc atcccgccca tgccctacat caccttcgag
     1561 caggcccccg gcgaggcgcc cggcaccccc ggcgagaagg tctacaaggc ccggcgcgag
     1621 tacttctaca cccagacgat caggcgcggc ctcttcgtcc agcccttcca ccactggtac
     1681 atcgcgcacc gccacaccga cgccgacctg cagaaggccc tcacggccat cgccgagagc
     1741 ctcgagctga cggcgaagaa gttcccggcg agttagggaa gaaagggaac cgcagagacg
     1801 cagaggcgca gaggggtagg ggaggatggc tgaatcattt gatgaaggcg agtttccgca
     1861 tatcgctttg acggaaagaa tcatcaaatg cgccatagct gtccacacag agcttggccc
     1921 gggccttttg gaatccatct atgaagaggc catgaccctt gagttgcagc ttgagggatt
     1981 atcctttgtc aggcaggtcg agcttccaat ggtctacaaa ggccatcgat tggcaggatc
     2041 gtacaggatc gacatggttg tggagaatga agtcgtggtt gagctgaaag ccgcgacaga
     2101 tattcatccc atccatgagg cccagctgct gacctatctt cgcctgggtg gatggaaggt
     2161 tgggcttctc ctcaatttca actaccggct cttgcgcgat ggcgtgaaga ggatcgtgct
     2221 ttcgtcgtga tctctctctg cgccaccgcg cctctgcgcc tctgcgcctc tgcggttttc
     2281 tacttggaaa ggagctgaca tatgcttgac ggaaagatag tgatcacctg cgcctgcacc
     2341 ggcgccgaga cgacaaaggc ccagaacccc gccctgccca tcacggccga ggagatcgcg
     2401 cagaccgcgg tcgaggcgag gcaggcaggg gcggccatcc tccacctcca cgtgcgggac
     2461 gccgagggca ggcccaccca gaagccggcc gtcttcaaga aggccatcga gctcatacgc
     2521 gccaagaccg acatcgtggt ggagatcacc acggggggcg cggtgggcga caccgccgag
     2581 gagcgcctgg ccccggtcac cgagctcgag cccgagatgg cgagcctcga ctgcgggacg
     2641 gtcaacttcg ggaacgacta catcgtcaac accctgcccg tcatgcggca gttcgccgag
     2701 gagttccgcc gccgcagggt gagggccacc ctcgagtgct tcgacctcgg ccacgtgtac
     2761 tcgagcggga tcctcctcaa ggagggcctc atggaggggc ccttccacta cggcttcgtg
     2821 ctcaacgtgc cgggggccgt gcccttcagc ccggccaacc tcgaggcctt cctcgccgcc
     2881 atccccgccg gggccaactg gacggtgatg ggcatcggca gggcctccct tcccgcccag
     2941 tacgcggcca tcgcccgcgg aggctggatc agggtgggct tcgaggacaa cgtctactac
     3001 tcgaaggggg tcctcgccaa gtcgaacgcc gagctcgtcg agcgcgccgc ccgccttgcc
     3061 agggaggccg gcctccagcc cgccagcccc gacgatgtgc gcaaggaact gcgcctgaga
     3121 gcctaagatc atcctgcgtt ctctgcggtg aaaccataag gagcttt
//
LOCUS       DASSQV010000179         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2034971, whole genome shotgun sequence.
ACCESSION   DASSQV010000179 DASSQV010000000
VERSION     DASSQV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034971"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VFL04_03115"
     CDS             complement(<1..990)
                     /locus_tag="VFL04_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7838725.1"
                     /translation="MTKPLKRGALALSISLSLAFMSPLMAEDGLVVTPEDCVARALGA
                     SQALKLDGARAQAAGARVSTMELQYLPSLVGSAGYTRSSPIDPGKISLPAGAVTLPAP
                     LEDSYNLRLAMQQPLFTGSRLSAGVDQARALYEAAKADERSRRRNTATMAEQAYWGLV
                     LAEETEAVVEENLTAMRAHRDDAQRRLAQGAGTRSELLAAMMRLSDAEARGSETRSSL
                     ALSRARLNLLIGLAWDAPTRTSPMPPEATAQEAKDTGELVAVALASRQDLAAMRARVG
                     AQEALLRVSRSALLPSVFLTGSFSLANPNPKAFPQRAGFESLWDLGLLVSIDLG"
     gene            complement(987..1925)
                     /locus_tag="VFL04_03120"
     CDS             complement(987..1925)
                     /locus_tag="VFL04_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7838726.1"
                     /translation="MAIEAQGLTRRFKGVEALKGIDIALEKGQSFALVGPDGAGKTTA
                     IRILCGLLKPSSGRALVFGSPPKDDRAKRRIGYLSQRFSLYGDLSIDENIAFFAEIHG
                     RRDYEKRRDRLLEFTRLKEFRGRQAGRLSGGMKQKLALACALIHEPELLLMDEPTTGV
                     DPVSRREFWSIVSELIHGGMTVLVTTPYLDEAERCGTVGLLNQGSFLDVDSPSAMKSG
                     FRWKVIEVVCGDTRRAARAIAEGGRLEGVQAFGDRLSVLAGDEAQARSLIAERLGRAG
                     IAAASVRLAAPSLENVFMALLGDKAAKAAGKGATPT"
     gene            complement(1949..2908)
                     /locus_tag="VFL04_03125"
     CDS             complement(1949..2908)
                     /locus_tag="VFL04_03125"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7838727.1"
                     /translation="MKSRIPLALTAILATLLSCSPSRPVTEASGTIEATEVRVSSTVQ
                     GRVLSMEAVEGASLKQGDPVAKIDHEALDLQLGQAKAGVELARSQLDLLLGGARSEDL
                     AQAQAAVDQANDNLRLAEADAKRMRELTASGSSTQRQLDDAEARLTAAKTQVQTSSQG
                     LKKLEAFARPEELRGARARLEQADWTARIIERSIRESDVSAPVDGVVAARLAEPGELA
                     SPGTGLILLEDMNRLYLTIYLPESELGRISLGAKAEVFVDSFPGRAFSGTLSYISPRA
                     EFTPKNVQTKDERVKQVFAVKIDLGTGEAVLKPGMPADARLAR"
     gene            complement(2905..>3152)
                     /locus_tag="VFL04_03130"
     CDS             complement(2905..>3152)
                     /locus_tag="VFL04_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838728.1"
                     /translation="IRSDIEPRLIAPLFIGMIEQVAQPAFLLNLPFGVSEVIDAISSM
                     LLGGILSEAGRQMFLSSKAAGITSSPAATGHEKGVSP"
BASE COUNT          446 a         1036 c         1146 g          524 t
ORIGIN      
        1 gccgaggtcg atgctgacga ggaggccgag gtcccatagg ctctcgaagc ccgcgcgctg
       61 ggggaaggcc ttggggttcg gattggccag gctgaaggat cccgtgagga agacgctggg
      121 caggagggca gagcgagaga ccctgaggag ggcctcctgg gctccgaccc tggcgcgcat
      181 tgccgccagg tcctgccgcg acgcgagggc aacggcgaca agctcgccag tatccttggc
      241 ctcttgggcc gtggcctcgg gcggcatcgg gctggtcctt gtgggggcgt cccaggccag
      301 gccgatgagg aggttgagcc tggccctcga aagggcgagg ctgctgcggg tctcggaccc
      361 cctagcctcg gcgtccgaga gacgcatcat cgccgcgagg agttcgctcc ttgtgcccgc
      421 tccctgcgcc aggcggcgct gggcgtcgtc gcggtgggcg cgcatcgccg tgaggttctc
      481 ctctaccacg gcctcggtct cctcggcgag gacgaggccc cagtaggcct gctcggccat
      541 ggtggccgtg ttgcggcggc ggctccgctc gtcggccttg gcggcctcgt aaagggcgcg
      601 ggcctggtcg acgccggcgc tgaggcggga gcctgtaaac agcggctgct gcatcgcgag
      661 acggaggttg tagctgtcct ccagaggggc cgggagggtc accgcgccgg ccggcaggga
      721 gatcttgccc gggtcgatcg ggctcgatct cgtatagccg gcgctgccga cgaggctcgg
      781 caggtactgg agctccatgg tcgagacccg ggctccggcc gcctgggccc tcgccccgtc
      841 cagcttgagc gcctggctcg cccccagggc ccgcgcgacg caatcctcgg gcgtcacgac
      901 gaggccatcc tcggccatga ggggtgacat gaaggcaagc gaaagggata tcgagagggc
      961 caaggccccg cgcttcaatg gcttggtcat gtcggtgtcg ctccttttcc ggcggccttg
     1021 gctgccttgt cgcccaggag ggccatgaac acattctcga ggctgggggc ggcgagcctc
     1081 accgaggccg ccgcgatccc ggcccggccc aggcgctcgg cgatgaggct ccgggcttgg
     1141 gcctcatccc cggcgaggac ggagaggcgg tcgccgaaag cctggactcc ttcgagtctc
     1201 ccgccctcgg ctatggcccg cgccgcccgc cgcgtgtctc cgcagaccac ctcgatcacc
     1261 ttccagcgga atccggactt catcgcgctc gggctgtcga catcgaggaa gctcccctga
     1321 ttcaggaggc ccacggtgcc gcagcgctcc gcctcgtcga ggtagggcgt ggtcaccagg
     1381 acggtcatcc cgccgtggat gagctcggag acgatggacc agaactcgcg gcgggagacg
     1441 gggtcgaccc ccgtcgttgg ctcatccatc agcaggagct cgggctcgtg gatgagggcg
     1501 caggcgaggg cgagcttctg tttcattccg ccggagagcc tgccggcctg gcggccgcgg
     1561 aactccttga gccgcgtgaa ctcgagcagg cggtcgcgcc gcttctcgta gtccctccgg
     1621 ccgtggatct cggcgaagaa ggcgatgttc tcgtcgatcg agaggtcgcc atagaggctg
     1681 aagcgctgcg agaggtagcc gatgcggcgc ttcgcccgat cgtccttggg ggggctgccg
     1741 aagacgaggg ccctgccgga actgggcttc agcaggccgc agagtatgcg gatggccgtc
     1801 gtcttccccg ccccgtccgg accgacaagg gcgaaggact ggcctttctc gagcgcgatg
     1861 tcgatgccct tcagggcctc gacccccttg aaccgccgcg tgaggccctg ggcctcgatt
     1921 gccatgtcgc ccacgtccag agctccgctc agcgcgcgag gcgggcgtcg gccggcatgc
     1981 ccggcttgag gacagcctcg cccgtgccca ggtcgatctt caccgcgaac acctgcttca
     2041 cccgctcatc cttggtctgg acattcttgg gcgtgaactc ggcgcggggg gagatataag
     2101 agagggtgcc ggagaaggcg cggcccggga aggagtcgac aaaaacctcg gctttcgcgc
     2161 caagggagat gcgtccgagc tcggactcgg gcaggtagat ggtgaggtat agccggttca
     2221 tgtcctcgag gaggatgagg cccgtcccgg ggctggcgag ctcgcccggc tcggcaaggc
     2281 gggccgcgac aacgccgtcg accggggccg agacgtcgga ctccctgatg gagcgctcga
     2341 tgatcctcgc cgtccagtcc gcctgctcaa gccgggcccg cgcgccacgg agctcctcgg
     2401 gcctggcgaa ggcctcgagc ttcttgagtc cctggctgct cgtctgtacc tgagtcttcg
     2461 ccgcggtgag gcgggcctcg gcgtcatcca gctgccgctg cgtggagctg ccgctcgcgg
     2521 tgagctcgcg catgcgtttc gcgtcggcct cggcgaggcg gaggttatcg ttcgcctggt
     2581 cgactgcggc ctgggcctgg gcgaggtcct cagacctggc cccgccgaga aggaggtcga
     2641 gctgggaacg ggccagctcg acgccggcct ttgcctggcc gagctggagg tcgagggcct
     2701 cgtggtctat cttcgccacg gggtccccct gctttaggct ggcgccctcg accgcctcca
     2761 tggaaaggac ccttccctgg actgtggagg agaccctcac ctcggtcgcc tcgattgtgc
     2821 ccgaggcctc ggtcacgggc ctggaagggc tgcacgagag cagtgtcgcg aggatggcgg
     2881 tcagggcgag ggggattcgg cttttcatgg ggatactcct ttctcgtggc cggtcgcggc
     2941 gggggagctc gtgatgcccg ccgccttgga gctcaggaac atctgccttc ccgcctcgct
     3001 caggatcccc ccgaggagca tcgagcttat cgcgtcgatc acctcgctca cgccgaaggg
     3061 aaggttgagg aggaaggcgg gctgggccac ctgctcgatc atgccgatga agagcggcgc
     3121 gatcagacgc ggctcgatgt ccgacctgat gt
//
LOCUS       DASSQV010000180         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2283846, whole genome shotgun sequence.
ACCESSION   DASSQV010000180 DASSQV010000000
VERSION     DASSQV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2283846"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1322)
                     /gene="trpS"
                     /locus_tag="VFL04_03135"
     CDS             complement(261..1322)
                     /gene="trpS"
                     /locus_tag="VFL04_03135"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET7838729.1"
                     /translation="MERKRILTGDRPTGPLHVGHYVGSIANRLRLQDQYECFFIIADL
                     HTLTTKPEKENIEALADNVRSAALDYLACGIDPAKSVIYLQSAVPEVIELSLYFQNLV
                     TVPRLSRIPSLKEMAQGAGIEEMPFGLLGYPVLQAADILLPRAHLVPVGKDNVAHVEI
                     TREIARRFNYLYGETFPVPDVMVSEFGSLVGTDGNAKMSKSLGNAIYLSDDEKTVRKR
                     VMSMYTDPKRVSADIPGTVEGNPVFQYHDAFNPDKAEVEDLKARYRAGTVGDVEVKEK
                     LAKALNAFLDPIRERRAKYESQSGLADEVIFDGTMRMREEARATLSAAKKAMGMSAVW
                     NRVARKAEDLKKKREKEGK"
     gene            complement(1550..>2272)
                     /locus_tag="VFL04_03140"
     CDS             complement(1550..>2272)
                     /locus_tag="VFL04_03140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7838730.1"
                     /translation="ALSNAAFFGAILFLPLWMQEVLGVSASSSGLAVTPMLLAYTLSS
                     VAGGQLLGKLRSMRAYAILAGLVAVAGSFLLIRVDRGMGQLPVIIAMVILGVGLGANT
                     PTLTIAAQNSVEGRYIGLATATNSFFRNLGSALGSAVMGSVFIRGLAAGLAGIDWGSS
                     PEALRSSLQDPKVLMSPAALQAIASSVPDGFRQTMARILDQIAGSLEHGIKAVFVVMA
                     AAAVLGLVPLVLLRGRRARPPA"
BASE COUNT          341 a          690 c          865 g          376 t
ORIGIN      
        1 cgcgggggcg aagaggttgg ggatggcgtc tgacagtgcc ccggttgtcc tgccgttgag
       61 cggcagggca ttggcgaggg cgttggcggt gagggcggcc gcgaggcaga gggcggcggc
      121 cagggccgaa gccctgaccg cgaggcggga accagtggcg atgggatcca tggacacctc
      181 cattcccgag cccagcgcag cccgcggcgc gccgggcctg gatcgcggga agccggcgaa
      241 aaatcgccgg gcttactccc ctacttccct tccttctccc gcttcttctt gaggtcctcg
      301 gccttcctgg cgacgcggtt ccacaccgcg ctcatcccca tggccttctt cgcagcgctc
      361 agggtggcgc gggcctcctc gcgcatccgc atcgtgccgt cgaagatcac ctcgtcggcg
      421 aggccggact gggactcgta cttcgcgcgg cgctcgcgga tcggatcgag gaaggcgttg
      481 agcgccttcg cgagcttctc cttgacctcg acgtcgccca cagtgccggc gcggtagcgg
      541 gccttgaggt cctcgacctc ggccttgtcg gggttgaagg cgtcgtggta ctggaagacg
      601 gggttaccct cgacggtgcc ggggatgtcg gcgctcaccc gtttggggtc ggtgtacatg
      661 gacatgacgc gctttctcac ggtcttctcg tcgtcggaga ggtagatggc gttgcccagg
      721 gacttggaca tcttggcgtt gccgtcggtg cccacgaggg agccgaactc cgagaccatg
      781 acatcgggca cggggaaggt ctcgccgtag aggtagttga agcggcgcgc gatctcccgg
      841 gtgatctcga cgtgggccac gttgtccttg cccaccggca cgaggtgggc ccgcgggagg
      901 aggatgtcgg cggcctggag gacggggtag cccaggaggc cgaagggcat ctcctcgatg
      961 ccggcgccct gggccatctc cttgaggctc gggatgcggg agagacgggg cacggtcacg
     1021 aggttctgga agtagagcga gagctcgatc acctcgggca cggccgactg cagatagatg
     1081 acgctcttgg ccgggtcgat gccgcaggcg aggtagtcga gggcggctga gcgcacgttg
     1141 tcggcgaggg cctcgatgtt ctccttctcg ggcttggtcg tgagggtgtg gaggtctgcg
     1201 atgatgaaga agcactcgta ctggtcctgc agacgcaggc ggttggcgat cgatccgacg
     1261 tagtgtccga cgtggagggg gcctgtgggg cggtcgcccg tgagaattct cttgcgttcc
     1321 atgctctgtc tccggtgtgg ggtgtcaggg aggattcctg cggctccgtc tctcaaagga
     1381 ccccgcgatc ctttgccgcc ctgggtcggc ccctattcct gggccgcggc ggacgggtcc
     1441 gcgcgtcgcc aggaccagtc gtgggggccg cgcctctgga ggccggtgcg gaggcttgcg
     1501 cccaaggtga ggctcatgcg ctcgagtgta gcgggagagg cccgcgccat caagcgggcg
     1561 gacgcgcccg ccttcccctg aggaggacga ggggtacgag gccgagcacg gcggcagcgg
     1621 ccatcacgac gaataccgcc ttgatgccat gctcgaggct gcccgctatc tggtcgagga
     1681 tgcgagccat ggtctggcgg aatccgtcgg ggaccgagga ggcgatggcc tgcagggcgg
     1741 cggggctcat gaggaccttg gggtcctgga ggctcgagcg cagggcctcg gggctcgagc
     1801 cccagtcgat acccgcgagg cccgcggcca ggccgcggat gaagaccgag cccatgacgg
     1861 ccgagcccag ggccgagccg aggttgcgga aaaaactgtt ggtggccgtg gcgaggccga
     1921 tgtagcggcc ctccaccgag ttctgggcgg ctatcgtgag ggtcggggtg ttggcgccga
     1981 ggccgacgcc gaggatgacc atcgcgatga tgacggggag ctgacccatg cccctgtcca
     2041 cccttatgag gaggaagctg cccgcgacgg cgacgaggcc ggccaggatc gcataggccc
     2101 tcatgctccg cagctttccg aggagctggc cgccggccac gctcgagaga gtgtaggcga
     2161 ggagcatggg cgtcaccgcg aggccgctcg acgaggccga gaccccgagg acctcctgca
     2221 tccacagggg caggaagagg atggcgccga agaaggcggc gttgctcagg gc
//
LOCUS       DASSQV010000181         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1536657, whole genome shotgun sequence.
ACCESSION   DASSQV010000181 DASSQV010000000
VERSION     DASSQV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536657"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..872)
                     /locus_tag="VFL04_03145"
     CDS             complement(210..872)
                     /locus_tag="VFL04_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET7838731.1"
                     /translation="MDARRFETHNERRYSTGEEIANAVTHGTGALLSVAALVLLVLSA
                     ALRGDALTVAAYAVFGSSLVLLFTMSTIYHALRAPRAKRVFEVLDHSSIYVLIAGTYS
                     AFCATALRGRAGLLLIILCWTFAAAGITFKAFFIDRYRLLATLGYVVMGWLIAPFLGQ
                     VRSSVPAGAFALLAAGGAAYTLGAALYSLKRVPWFHAVWHLFVLAGAACHVASALIIV
                     GA"
     gene            complement(1007..1780)
                     /locus_tag="VFL04_03150"
     CDS             complement(1007..1780)
                     /locus_tag="VFL04_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838732.1"
                     /translation="MNPVGALGKKVFFLYPPPVLAEIMEGLAKREFEVYTARSHKKLL
                     KVLAQEPESIVFINIDDESLDDGEWQDYVRGLREGETTAGVGVGILTLNEPSPELRER
                     YLMDIQVPCGFVVLKIGATKTTEILVKTLEANEARGRRKFVRAICSPGSGHFAATHEG
                     KSLNGSVSDLSSAGFAVGFEDKTQLKVGTVVRGVLLTVKGVRIGADAFVAAMRTNGES
                     TTHVLMFVPGANDEHRLEKLRSLVFRINQTAMDSLMAGA"
     gene            complement(1846..2313)
                     /gene="msrB"
                     /locus_tag="VFL04_03155"
     CDS             complement(1846..2313)
                     /gene="msrB"
                     /locus_tag="VFL04_03155"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7838733.1"
                     /translation="MVMKRSEADSVCYVEPSEELRAKLKPEQFAVLVKGATEPPFRNP
                     YWNNHEKGVYVDAIDGTPLFSSREKFDSGSGWPSFWQPLDEGRLVLVEDNSLGMHRVE
                     VRAKASGGHLGHVFEDGPEPTGLRYCMNSASLRFIPQARLEEEGYGELAALFA"
     gene            complement(2304..>3001)
                     /locus_tag="VFL04_03160"
     CDS             complement(2304..>3001)
                     /locus_tag="VFL04_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838734.1"
                     /translation="AEAAGPMLSYWSSIEKAWRIDLEVEAGDGEPFLPAPWSAVDEPP
                     ADWQDPWMASADRLGRKRDGCEELFDCLRAAEAALAEAALIAKDRAGEAWAEAVRGEE
                     AEYRIAGALLELDCARLSAYHEAALGDRRTARDLAYLAESTMDALLAALALSPDRRAR
                     REGAFLIFLFYGLRLRLMKREASGKPVARAAGRTATFLELALRAARVAGVWEPRRRAS
                     GGKSAGAKESSWS"
BASE COUNT          484 a         1002 c         1063 g          452 t
ORIGIN      
        1 cgacccaagg tatttcgtgc aacgcgtcga ggaggcggtc tggaacgacc tccagtacat
       61 cagggtgaac ggggcggccg tcggcggcct cgtcggcctc ctcatcgccg tcttcaccgc
      121 catcttcagg ccctgaggcc cggcgggccg ccgcgcggac agggctggcc cggcgggcgc
      181 ccgcgcgaac agggctggcc aggcgcgcct caggcgccga cgatgatgag ggcagaggcg
      241 acgtggcagg ccgcgccggc cagcacgaag aggtgccaga ccgcgtggaa ccagggcacc
      301 cgcttcaggg agtaaagggc ggcgccgagg gtgtaggccg cccctccggc cgcgagcagg
      361 gcgaaggccc cggccggcac cgaggacctc acctggccga ggaagggggc gatgagccag
      421 cccatgacga cgtagcctag ggtcgcgagg aggcggtagc ggtcgatgaa gaaggccttg
      481 aaagtgatcc ccgcggcggc gaaggtccag cacaggatga tgaggaggag gccggcgcgg
      541 ccgcggaggg ctgtcgcgca gaaggcgctg taggtccccg cgatgaggac gtagatcgag
      601 gaatggtcga ggacctcgaa gacgcgcttc gcgcgcgggg ccctgagcgc atggtagatg
      661 gtcgacatgg tgaacaggag gacgagggag gagccgaaga cggcgtaggc cgcgaccgtg
      721 agggcgtctc cccgcagggc cgctgacagg acaaggagga cgagggcggc caccgagagc
      781 agggcccccg tcccgtgggt caccgcgttc gctatctcct cgcccgtcga gtagcgcctc
      841 tcgttgtggg tctcgaatcg ccttgcatcc attctgctca ctccgatgat ccgcaccttc
      901 ggccgcatcc gcgcccgaac cgcccagtac aggctgcagg agcctagacc cggaagacgt
      961 tgatcgggtt gcgggggagc ggcccaagac tgggagccca atgggctcag gcgccggcca
     1021 tgaggctgtc catcgccgtc tggttgatgc ggaagacgag gctgcggagc ttctcgaggc
     1081 ggtgctcgtc gttggccccg gggacgaaca tgaggacgtg ggtcgtcgac tcgccgttgg
     1141 tccgcatcgc cgcgacgaag gcgtcggcgc cgatcctgac tcccttgacc gtgaggagca
     1201 cgcctctgac caccgtcccc accttgagct gggtcttgtc ctcgaagccg acggcgaagc
     1261 ccgccgagct gaggtcggag accgagccgt tcagggactt gccctcgtgg gtggcggcga
     1321 agtgccccga gcccggggag cagatggcgc ggacgaactt cctccgcccc cgcgcctcgt
     1381 tggcctcgag ggtcttcacg aggatctcgg tggtcttggt cgccccgatc ttgagcacca
     1441 cgaagccgca gggcacctgg atgtccatga ggtacctctc ccgcagctcg gggctcggct
     1501 cgttgagggt gaggatgccc acgccgaccc cggccgtggt ttccccttcc ctgaggccgc
     1561 gcacgtagtc ctgccattcg ccatcgtcga gggattcgtc gtcgatgttg atgaagacga
     1621 tggattcggg ctcctgggcg aggaccttga gcagcttctt gtgggacctc gccgtataga
     1681 cctcgaactc ccgctttgcg agcccctcca tgatctccgc gaggacgggg ggagggtaaa
     1741 ggaagaagac cttcttgccg agggcgccaa ctgggttcac gacatacacc ctagcgcgcg
     1801 cccggagctg ccgcaatggc cgggggcgaa aagccccgga aagcctcagg cgaagagggc
     1861 agccagctcg ccgtagccct cctcctcgag ccgggcctgg gggatgaagc gcagggaggc
     1921 cgagttcatg cagtagcgga gccccgtcgg ttcgggaccg tcctcgaaga catggccgag
     1981 gtggcccccc gaggccttgg ccctgacctc gaccctatgc atgccgaggg aattgtcctc
     2041 gacgaggacg agacggccct cgtccaaggg ctgccagaag ctcggccagc ccgagcccga
     2101 atcgaacttc tccctcgagg agaacagggg ggtcccgtcg atggcgtcga cgtagacccc
     2161 cttctcgtgg ttgttccagt aggggttcct gaagggcggc tcggtcgccc ccttcacgag
     2221 gacggcgaac tgctcgggtt tgagcttcgc ccgcagctcc tcgctcggct cgacatagca
     2281 gacgctgtcg gcctcggacc gcttcatgac catgatgact ccttcgcccc ggcgcttttc
     2341 ccgccacttg cgcggcgcct cggctcccag acccccgcga ccctggccgc cctcagggcg
     2401 agctcaagga aggtcgccgt cctgccggcc gcccgcgcca cgggcttgcc cgaggcctcg
     2461 cgcttcatga gcctgagcct gagcccgtaa aaaaggaaga tgaggaaggc gccctccctc
     2521 cgtgcccgtc tgtcggggct caaggccagg gcggcaagga gggcgtccat ggtggactcc
     2581 gcgagatagg cgaggtccct cgcggtccgg cgatcgccca gggcagcctc gtggtaggcc
     2641 gagagccggg cgcagtcgag ctcgaggagg gcccccgcga tccggtattc ggcctcctcg
     2701 ccccgcaccg cctcggccca ggcctcgccg gccctgtcct tggcgatgag ggcggcctcg
     2761 gccagggcag cctcggccgc gcggaggcag tcgaagagct cttcgcagcc gtctctcttg
     2821 cgcccgaggc gatcggcgct ggccatccat gggtcctgcc agtcggcggg cggctcgtcg
     2881 acagccgacc agggagcggg aaggaagggc tcgccgtcac cggcttcgac ctcgagatcg
     2941 atcctccagg ccttttcgat cgagctccag taggaaagca tcgggcctgc ggcctcggca
     3001 t
//
LOCUS       DASSQV010000182         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1536899, whole genome shotgun sequence.
ACCESSION   DASSQV010000182 DASSQV010000000
VERSION     DASSQV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536899"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VFL04_03165"
     CDS             <1..1220
                     /locus_tag="VFL04_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HET7838735.1"
                     /translation="AALKDIAQGEGDLSVRLQIATRDETGELAGYFNDFICKLELAVM
                     RIKAVSRSGAALGDELAANSSQVSAAIIEFGSTLASMADRVATMDRSVESVDGAVRGI
                     SEGIVTVGALIARQTEAVAASTAAAQDIIRGVGSLATEAEAKRTVADQLSARAREGET
                     SMARLLEAVKEIGSYAERIAGMVAVINDVAERTNLLAMNAAIEAAHAGDRGRGFAVVA
                     DEIRKLAESTGRNASAIREQLRSVVEKIDDTASGAEQAGSSIRGMIDGSATAASSFRE
                     VIEGLKTLAARTSEGLEGLRSLVVSAESAKAASADIEGRSRLIMKEVEDLTSVSRESR
                     AGFEEMNRGIGEMTQAAEALSNLGSQNSRNIAALDEEFGRFRTSEDLCGTLEPALATD
                     AEAADEAAGGGEP"
     gene            1217..2212
                     /gene="pdxA"
                     /locus_tag="VFL04_03170"
     CDS             1217..2212
                     /gene="pdxA"
                     /locus_tag="VFL04_03170"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET7838736.1"
                     /translation="MRAARAGGPRIALTMGDPAGIGPEIVARALADRALRGTARFVVY
                     GDARVMERAVALTGAGLAVRGTSVGQAGGFGPDELPVVDLANADPAAFEPGRVSGLCG
                     RASWEYIEAAAKAALAGEVDAIATAPINKEAIVAARVPFIGHTEMLAGVAGVADPLTM
                     FETLGLRVFFLTRHVSLKVACELVKRPRIVDYLERCSGALAGLGVVGEIAVAGLNPHC
                     GEHGLFGDEEEREVRPAVEEARSRGLAVAGPIGADSVFHQAKTGRYAAVLSLYHDQGH
                     IACKTLDFERTVSLTLGLPFLRTSVDHGTAFDLAWKGEASAVSMVEAARAAARYA"
BASE COUNT          363 a          893 c          952 g          331 t
ORIGIN      
        1 cggcggccct gaaggacata gcccagggcg agggggacct cagcgtgagg ctccagatcg
       61 cgacgcggga cgagaccggc gagctcgccg gctacttcaa cgacttcatc tgcaagctcg
      121 agctggcggt catgcgcatc aaggccgtca gccggagcgg ggctgccctc ggcgacgagc
      181 tcgcggccaa ctcctcccag gtctcggccg ccatcatcga gttcggctcg accctggcgt
      241 ccatggccga ccgggtggcg acgatggaca ggagcgtcga gagcgtcgac ggggccgtgc
      301 gcggcatctc cgagggcatc gtgaccgtgg gcgcactgat cgcgcgccag accgaggcgg
      361 tcgccgcctc gaccgccgcg gcccaggaca tcatccgcgg ggtcggctcg ctcgccaccg
      421 aggccgaggc caagcgcacg gtggccgacc agctctcggc gcgggcccgc gagggcgaga
      481 cctcgatggc ccgcctcctc gaggcggtca aggagatcgg ctcctacgcc gagaggatcg
      541 ccggcatggt cgccgtcatc aacgacgtgg cggagcggac gaacctcctc gcgatgaacg
      601 ccgccatcga ggcggcccac gccggggaca ggggcagggg cttcgccgtg gtcgccgacg
      661 agatccgcaa gctcgccgag tcgaccggcc gcaacgccag cgcgatccgg gagcagctgc
      721 gctcggtcgt cgagaagatc gacgacacgg cctcgggggc cgagcaggcc ggctcctcga
      781 tccggggcat gatcgatggc tcggccacgg cggcctcgag cttccgcgag gtgatcgagg
      841 gcctcaagac cctcgcggcg cgcacctccg agggcctcga gggcctgcgc tccctcgtcg
      901 tttccgccga gtcggcgaag gccgcgagcg ccgacatcga gggcaggtcg aggctcatca
      961 tgaaggaggt cgaggacctc acgagcgtct cgcgcgagag cagggcgggc ttcgaggaga
     1021 tgaaccgcgg catcggcgag atgacccagg ccgcggaggc cctctcgaac ctcggcagcc
     1081 agaactccag gaacatcgcg gccctcgacg aggagttcgg ccgcttccgc acgagcgagg
     1141 acctctgcgg caccctcgag ccggctctcg ccaccgacgc ggaggccgcg gacgaggccg
     1201 ccgggggcgg ggagccatga gggccgcaag ggctggcggc ccgaggatcg ccctcacgat
     1261 gggcgatccc gcggggatcg ggcccgagat cgtggcgagg gccctggccg accgggccct
     1321 gcgcgggacc gcgcgtttcg tcgtctacgg ggacgcgcgc gtgatggaaa gggccgtggc
     1381 cctgaccggc gcgggcctgg cggtgcgggg cacgagcgtg gggcaggccg gcggcttcgg
     1441 cccggacgag ctccccgtgg tcgacctggc caacgccgac ccggccgcct ttgagccggg
     1501 ccgggtttcc gggctctgcg gccgggcctc atgggagtac atcgaggcgg ccgccaaggc
     1561 cgccctcgcc ggcgaggtcg acgcgatcgc gacggcgccc atcaacaagg aggcgatcgt
     1621 cgccgctcgg gtgcccttca tcggccacac cgagatgctc gccggcgtcg ccggcgtggc
     1681 cgaccccctg accatgttcg agaccctcgg gctccgcgtc ttcttcctca cgaggcacgt
     1741 ctcgctgaag gtggcctgcg agctcgtgaa gaggccgcgc atcgtggact accttgagcg
     1801 ctgctcaggc gccctcgccg ggcttggcgt cgtcggcgag atcgccgtgg ctggcctgaa
     1861 cccccactgc ggggagcacg ggctcttcgg cgacgaggag gagcgcgagg tgaggcccgc
     1921 cgtcgaggag gccaggtccc ggggcctggc cgtggccggg ccgatcgggg ccgactcggt
     1981 cttccaccag gccaagaccg ggcgctacgc cgccgtcctc tcgctctacc atgaccaggg
     2041 ccacatcgcc tgcaagaccc tcgacttcga gcggacggta tcgctgaccc tcggccttcc
     2101 cttcctcagg acctcggtgg accacggcac cgccttcgac ctggcctgga agggcgaggc
     2161 gagcgcggtg agcatggtcg aggccgcccg cgccgccgcc aggtacgcct aggcggcccc
     2221 cgatcgtgag ggccggggcc gggggctggc ccgtgcttgg gacgcgggtc ccgggcttgt
     2281 ggtccgtgcc tgggacacgg ggccgggctt gtggtccggg gcccgcctcg acgagggcca
     2341 gcattgcggg tatcatggac atcatgccgc gttcccacga tgtcgagcgt accatagaca
     2401 agctcgtcgc cggcggcgac gggctcgcct tccgcgaggg gaaggccgtc ttcgtcccct
     2461 tcgccctccc cggcgagagg gtcagggcga gggtcgtcga ggagcggaag gacttcgcgc
     2521 gcgcggccct cgtgaaggt
//
LOCUS       DASSQV010000183         4796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2035748, whole genome shotgun sequence.
ACCESSION   DASSQV010000183 DASSQV010000000
VERSION     DASSQV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035748"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFL04_03175"
     CDS             <1..532
                     /locus_tag="VFL04_03175"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET7838737.1"
                     /translation="ALAASGARADTAQKASAAVRPWYATGFEELGFKVFPEPVPVDDF
                     SARMLSGGSSKLSESRGKIVLLNFWATWCPPCREEIPSIQTLWTAMKGKSFSIMGVSI
                     GETEATVRDFVAKNKMSYPVFLDSSGEIGTAFGARSIPTTYVIDKTGRAIAGIVGGAP
                     YDSPKAAALFAELAAR"
     gene            529..1464
                     /locus_tag="VFL04_03180"
     CDS             529..1464
                     /locus_tag="VFL04_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET7838738.1"
                     /translation="MSDPGILPALGAGLLTFLSPCVLPLIPGYLSFISGYSLAEIREG
                     RLRFRVLGRTAVFIAGFTIVFVAMGLVFAGGGLLLGGLSRAFTIGAGALIALLGLNLI
                     FDFLKFLDLEARFHAKSRPSGYAGAFLVGIAFAAGWSPCVGPILAAILVLAARDSNAG
                     RAALLLGSYSLGLALPFLAAGLFFDRTKALMDGFRRHARLVRVGSGSLLVALGLAMAF
                     GKLGLLSGVAPRLGMALAESLVSNPTPLRIGVASAWALLAALTMGLPLLRGRRVLTAP
                     RIVSSGLFAALAAGELAGLWSLARLISQWLLFRGA"
     gene            complement(1557..1907)
                     /locus_tag="VFL04_03185"
     CDS             complement(1557..1907)
                     /locus_tag="VFL04_03185"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7838739.1"
                     /translation="MADKRRLARFPVGGRVSLRSSGLTGRLCDISERGIGVMLQGPPS
                     EPIGKRERILISPEPHRTGSIDFEAEMKWFNEGPRSTILGYEIKSFRDERQRDKFRRL
                     ALDRHSGTDPSVPE"
     gene            complement(1962..2948)
                     /locus_tag="VFL04_03190"
     CDS             complement(1962..2948)
                     /locus_tag="VFL04_03190"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HET7838740.1"
                     /translation="MVDTPKPSSRGLRARFLGAIMLGVRSAGRTTVFLLKIIVPVSLA
                     VAILGWSGILERIAAFTAPLMRLLGLSGDAALVFISSILLNIYSALAVAGSLALDHRQ
                     TTILAIMCLTAHNLIMETAVMRKAGSSAWKMVALRLVMAVVVGWLFNLLLPRALGASA
                     MAAVGPASRPELLAMLLAWAASTLRLALRIIVIVLAIMIAQRLLEEFRVMDWLAGLLS
                     PFMRLLGLPRRASFLWIVINVVGYAYGAGIIVEQIETGGMTKEEGDLLNHHAGMCHSL
                     MEDTALFLAAGCPLVWITLPRLALAVAVVWLELLRRRLFRSGPGVLGPRVGA"
     gene            complement(2999..4540)
                     /locus_tag="VFL04_03195"
     CDS             complement(2999..4540)
                     /locus_tag="VFL04_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET7838741.1"
                     /translation="MRGSRAAGMILAGLPVEGQGAAGSEEIIDRYASAGIAAFLFPGL
                     ALLDPERLGSLAAKARQACSAAGLGRPLIAIGGSPGPGFGLPPWPRGPSPLGIAALGS
                     RQAAKRAGRLLGLELAACGVDLVLAPRLDLATDPKDSAGVLDGFGQDPRLASALGAAF
                     VRGLGSAGVAACAGRFPGLGSTCAECFDGLSFVSLPAERLEAVEMRPFAAAVRGGIDA
                     ILVGRAFVPAFEPERIPAARSARVIEGRLRAQLGFRGLVIGDGLGGEKARDNAVLLGA
                     LAGCDLCLVADPDEALSAASALAAALASGEMPEPRLHVATRRLGRFLSHWPSRPESGA
                     RPPHGRLGLRVERDRSEALAVLRGGPFEALPAEGLLVAVFVPPAGSPDAAETEAALEA
                     LGSAFPGAQILALPNDPDFGKADSVAALLSEKGFRAAAILTCDAHLHPAQESLARLIE
                     DAVPRSIVVAMRDPYDAAFFPRAEALVAAFGFSAGGAAAVARLLSGRAQAAGRSPVEV
                     IGIEL"
BASE COUNT          721 a         1694 c         1710 g          671 t
ORIGIN      
        1 cgcccttgcc gcgtccggcg cccgagccga cactgcgcag aaggcctccg cggccgtccg
       61 cccctggtac gccaccggct tcgaggagct cggcttcaag gtcttccccg agcccgtccc
      121 tgtcgacgat ttctccgcca ggatgctctc gggcgggagc agcaagcttt cggaatccag
      181 gggcaagatc gtcctgctca acttctgggc cacctggtgc ccgccctgcc gcgaggagat
      241 cccctcgatc cagaccctct ggaccgccat gaagggcaag agcttttcca tcatgggcgt
      301 gagcatcggc gagaccgagg ccacggtgag ggacttcgtg gccaagaaca agatgagcta
      361 ccccgtcttc ctcgacagct cgggggagat cgggactgcc ttcggcgccc ggtccatccc
      421 caccacctat gtcatcgaca agacgggcag ggccatcgcc ggcatcgtgg gcggagcccc
      481 ctacgactcg cccaaggccg ccgccctctt cgccgagctc gccgcgcgat gagcgatccg
      541 ggcatcctgc cagctctggg ggcagggctc ctcaccttcc tgagcccctg cgtcctgccc
      601 ctgatacccg gctacctctc cttcatctcg ggctacagcc ttgcggagat cagggagggc
      661 cggctgaggt tccgcgtcct cgggaggacg gccgtcttca tcgccggctt caccatcgtg
      721 ttcgtcgcca tgggcctcgt gttcgccggt ggcggactcc tcctcggcgg gctttcgcgg
      781 gccttcacca tcggggccgg ggccctcatc gccctcctcg gactcaacct gatctttgat
      841 ttcctgaagt tcctcgacct cgaggcccgc ttccacgcca aaagccgccc aagcggctac
      901 gccggggcct tcctcgtggg gatcgccttc gccgcgggct ggtcgccctg cgtcggcccc
      961 atcctcgctg cgatactcgt gctcgcggcc agggactcga acgccggccg cgcggccctg
     1021 ctcctcgggt cctactccct cggcctcgcc ctgcccttcc tcgctgccgg gctcttcttc
     1081 gaccggacca aggccctcat ggatggcttc agacgccacg cccggctcgt ccgcgtcgga
     1141 tcgggaagcc tcctggtcgc gctcggcctg gccatggcct tcggcaagct cggccttctg
     1201 agcggggtcg cgccgaggct gggcatggcc ctcgccgaga gcctcgtctc caacccgact
     1261 cccctccgga tcggcgtggc ctcggcctgg gccctgctcg ctgccctgac catgggtctg
     1321 cccctgctca ggggccggag ggtcctgacc gcgccccgca tcgtctcgtc agggctcttc
     1381 gccgccctgg cggcggggga gctggcgggg ctctggtccc tcgcccgcct catctcccag
     1441 tggctgcttt tccggggggc ctagcggggc ctccgccggg tacggcgccg ccgaagacgg
     1501 cgcagccgag gacggccccg ccgtggacgg cgccgctgcg ggcgaggcga ccgccctcac
     1561 tcgggcaccg acggatcggt tccggaatgc ctgtccaggg cgagcctgcg gaacttgtcc
     1621 ctctggcgct cgtcgcggaa ggacttgatc tcgtagccga ggatggtcga gcgtggcccc
     1681 tcgttgaacc acttcatctc ggcctcgaaa tcgatcgagc cggtcctgtg aggctcgggg
     1741 ctgatgagga tgcgctcccg cttgccgatg ggctcgctgg gcggcccctg cagcatgacc
     1801 ccgatgcccc gctcgctgat gtcgcagagc ctgccggtga ggcccgagct ccgcagcgac
     1861 accctgcccc cgaccgggaa tcgggctaga cggcgtttgt ctgccatggc ataccccctc
     1921 gagccccaag gtaccgagga cggccgatgg gggaaagccg attatgcgcc gactctcggg
     1981 ccgaggacgc cgggtccaga ccggaaaagc cggcgcctga gcagctcgag ccagaccacg
     2041 gcgacggcaa gggccagacg gggcagggtg atccagacaa gggggcagcc ggcggcgaga
     2101 aagagggccg tgtcctccat gagggaatgg cacatgcccg cgtggtggtt gagaaggtcg
     2161 ccctcctcct tcgtcatccc tccggtctcg atctgctcga cgatgatccc ggctccgtag
     2221 gcgtagccca ccacgttgat cacgatccac aggaagctcg cgcgcctcgg gaggccgagg
     2281 agccgcatga agggagaaag gaggccggcg agccagtcca tgaccctgaa ctcctcgagg
     2341 aggcgctggg cgatcatgat ggccaggacg atgacgatga tccgcagggc cagcctgagc
     2401 gtcgaggccg cccaggccag aagcatggcg aggagctcgg gccgcgaggc ggggccgacc
     2461 gccgccatgg cggaggctcc gagcgcgcgc gggaggagga ggttgaagag ccagccaacc
     2521 acgaccgcca tgacgagcct cagggccacc atcttccagg ccgaggatcc ggccttcctc
     2581 atgaccgcgg tctccatgat gaggttgtgc gccgtcagac acatgatggc gaggatggtc
     2641 gtctggcggt ggtcgagggc cagggagccg gcgacggcga gggcgctgta gatgttgagg
     2701 aggatgctcg agatgaacac cagggccgca tcgcccgaaa gccccaggag acgcatgagc
     2761 ggagccgtga aggcggcgat cctctcgagg atcccgctcc agcccaggat ggcgaccgcg
     2821 aggctgaccg ggacgatgat cttcaggagg aagaccgtcg tccttccggc gctcctgacg
     2881 ccgagcatga tggcgccgag gaacctcgct ctgaggcccc gtgaggaagg ctttggggtg
     2941 tcgaccatgg gggaggactc tagacgccgt cgcgccggca tgtcaaggaa ggccggccct
     3001 agagctcgat cccgatgacc tcgacggggc tcctgcccgc ggcctgggcc cggccggaga
     3061 gcaggcgggc cactgccgcc gcccccccgg cgctgaagcc gaaggcggcc acgagggcct
     3121 cggcccgtgg gaagaaggcg gcgtcgtagg gatcgcgcat cgcgacgacg atcgacctcg
     3181 ggacggcgtc ctcgatgagc cgggcgaggc tctcctgggc cggatggagg tgggcgtcgc
     3241 aggtgaggat cgcggcggcg cggaagcctt tctccgacag gagagcggcc acggaatcgg
     3301 ccttgccgaa atccggatcg ttggggaggg ccaggatctg ggcgccgggg aaggccgagc
     3361 cgagggcctc tagggcggcc tcggtctcgg cggcgtcggg cgagccggcc ggagggacga
     3421 agacggccac caggagcccc tctgccggga gggcttcgaa gggcccgccc ctgagcaccg
     3481 caagggcctc ggaacggtct cgctcgaccc tcagccccag gcggccgtgg gggggcctcg
     3541 ccccggactc cggtcgactc ggccagtgcg agaggaaacg ccccagccgc cgcgtcgcca
     3601 catggaggcg gggctcgggc atctcccctg aggcgagggc cgcggccagg gcagaggccg
     3661 cgctcagggc ctcatcggga tcggcgacga gacagaggtc gcagccggcg agggcgccaa
     3721 gaaggacggc gttgtccctg gccttctccc cgccgagccc gtcgccgatg acgaggcccc
     3781 taaagcccag ctgggccctg agcctgcctt cgatgacccg ggcgctcctt gcagcgggga
     3841 tccgctctgg ctcgaaggcc ggcacaaagg ccctgcccac gaggatagcg tcgatgcccc
     3901 cgcggacggc ggcggcgaag ggccgcatct cgacggcctc gaggcgctcg gccggaagcg
     3961 agacgaagga gagcccgtcg aagcactcgg cgcaggtcga gccgaggccg gggaagcgcc
     4021 cggcgcaggc cgcgacaccg gccgagccga ggccgcgcac gaaggccgcc ccgagtgccg
     4081 aggcgaggcg ggggtcctgg ccgaagccgt cgaggacgcc cgcgctgtcc ttcgggtcgg
     4141 tggcgaggtc aagacggggg gcaaggacga ggtcgacccc gcaggcggcg agctcaagac
     4201 cgaggagcct gcccgcgcgc ttcgccgcct gcctcgagcc gagggccgcg atgccgaggg
     4261 gactggggcc gcggggccag gggggaaggc cgaagccggg accgggcgag ccgccgatcg
     4321 cgatgagggg cctgccgagt cctgccgccg aacaggcctg cctggccttc gcggccaggg
     4381 agccgaggcg ctcgggatcg aggagggcca ggcccgggaa gaggaaggcc gcgatccccg
     4441 ccgaggcgta gcggtcgatg atctcctcgc tgccggcggc gccctggccc tcgacgggga
     4501 ggcccgcgag gatcatcccc gccgcccgag agccgcgcat catccgcaag ccttcatggc
     4561 cccatagtat tccatcgtcg ggcaaaacgg aagcgaaggg ccgcggctcg gccgcgccac
     4621 tcgactcaag gccgacgcgc ggcgcagagc cgctccacca tcgtcctcag atcggagtcc
     4681 cccgcgaggg cctcgatgcc caccggcatc ctgtagcgcc agttgcccga tccgacggtg
     4741 ccggggacgt tgatgcgctc cctccccggg tcggccggcc tcttgtcgga gctcat
//
LOCUS       DASSQV010000184         3943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2035806, whole genome shotgun sequence.
ACCESSION   DASSQV010000184 DASSQV010000000
VERSION     DASSQV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035806"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /gene="thrS"
                     /locus_tag="VFL04_03200"
     CDS             complement(<1..1696)
                     /gene="thrS"
                     /locus_tag="VFL04_03200"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET7838742.1"
                     /translation="MSAPVETIRHSLSHILAEAVLKLYPGTKLGIGPAIENGFYYDFQ
                     LPAPIQNEDLAAIEKEMRRIVAGGHEFVRREIPKDEARRLFADQPFKVELIEALEDGT
                     ISTYEQDGFVDLCRGPHVASTREIRPDAFKLKTVAGAYWRGDEKRPMLTRIYGLGFSL
                     KAELEAYLKMLEEAEKRDNRRLGKELDLFSTHEEAGAGLIYWHPKGGRFRVALENWWR
                     DEHYENGYEILFTPHIGKSWLWETSGHLGFYKENMYSPMKIDEDNYFAKPMNCPFHIM
                     IYKSQTRSYRDLPLRWAELGTVYRYERAGALHGLMRVRGFTQDDAHIFCTPEQVEDEI
                     LEVLRFSLHMLRSLGFTEINAYLSTKPKGDGSVGAPERWEQATESLRKAIEKEGLKYE
                     VDEGGGAFYGPKIDLKVKDAIGREWQLSTIQFDFNMSARFEMEYVDADGQRKLPYMVH
                     RALLGSIERFFGVYLEHTGGAFPVWLCPEQAAVIPVGEAFMDYAKKVAAELKAVGIRA
                     RAMLSDERMNAKIRDAQNQKIPYMLVVGQKELDEGAVSIRHRDGRQENGVALAAFKA"
     gene            complement(2140..>3943)
                     /locus_tag="VFL04_03205"
     CDS             complement(2140..>3943)
                     /locus_tag="VFL04_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7838743.1"
                     /translation="NGLLDFAKLEAGKMALCPQKTDLVAALEYYLSTVQSAAEARGLE
                     LGFRSSCGPCLFVSLDRHLFEKAFYNLLSNALKFTPRGGSIAVSLSCEGGDFLLSVRD
                     SGIGIAADKLESIFERFNQVDPSSSRNYEGTGIGLSLTKDIVAIHGGSISVTSAPGQG
                     SSFLLRMPIGEVSAEEQAEDIRSVRASMLSDLVPARHEGGGAATEAAAAGAGAAAAGS
                     EADAAGAGADAAAAGAAGPAPMGPSEDGRARVLVVEDNPEMREYLGSILARDFRVETA
                     SDGIEGLEAARLRAPDIVVSDVMMPRMDGYRLCEELKADPATAELPVILLTARTETSM
                     RLDGYRSGADDYVGKPFNAEVLVSKIRVFVTREEMRKRLEELAAELRASNEDLELRVR
                     ERTAKLEEQFYQILDSLATALEEKDHYTQGHSRRVERYSRLLAADLGLGEEDRHVLSV
                     AASLHDIGKIGIPESILNKAGPLSSEEYELIKKHPEKGARILSPFSDLGRVIDVAVKH
                     HERLDGRGYLGCAAADIPMLSRILSVADAFDAMTSSRAYRGAMTAAEALAEIARNKGS
                     QFEGRVVEALAALLDRGGLEAEAEGRGAAEAGAD"
BASE COUNT          563 a         1303 c         1396 g          681 t
ORIGIN      
        1 tggccttgaa ggcggccagg gcgaccccgt tctcctgtct gccgtcgcgg tggcggatcg
       61 agacggcgcc ctcgtcgagc tccttctggc ccaccacgag catgtagggg atcttctggt
      121 tctgcgcgtc gcggatcttg gcgttcatcc gctcgtcgct gagcatcgcg cgcgcccgga
      181 tgccgacggc cttgagctcg gcggccacct tcttcgcgta gtccatgaag gcctcgccca
      241 cggggatcac cgccgcctgc tcggggcaga gccagacggg gaaggccccg ccggtgtgct
      301 cgagatagac gccgaagaaa cgctcgatgg acccgaggag ggcgcggtgc accatgtagg
      361 gcagcttgcg ctggccgtcg gcgtcgacgt actccatctc gaaccgcgcc gacatattga
      421 agtcgaactg gatggttgag agctgccact cgcggccgat cgcgtccttg accttgaggt
      481 cgatcttggg cccgtagaag gccccgccgc cctcgtcgac ctcgtacttc aggccctcct
      541 tctcgatggc cttgcgcagg gactcggtgg cctgctccca gcgctcgggc gcgcccacgg
      601 agccgtcgcc cttgggcttg gtggagaggt aggcgttgat ctcggtgaag ccgagggacc
      661 tcagcatgtg gagcgagaag cgcaggacct cgaggatctc gtcctcgacc tgctcggggg
      721 tgcagaagat gtgggcgtcg tcctgggtga agccgcgcac gcgcatgagg ccgtggaggg
      781 cccccgcgcg ctcgtagcgg tacacggtcc cgagctcggc ccagcggagg ggcaggtcgc
      841 ggtaggagcg ggtctggctc ttgtagatca tgatgtggaa ggggcagttc atcggcttcg
      901 cgaaatagtt gtcctcgtcg atcttcatcg gcgagtacat gttctccttg tagaagccga
      961 ggtggcccga ggtctcccag agccagctct tcccgatgtg gggggtgaag aggatctcgt
     1021 agccgttctc gtagtgctcg tcgcgccacc agttctcgag ggccacgcgg aaacggccgc
     1081 ccttggggtg ccagtagatg aggcccgcgc ccgcctcctc gtgggtcgag aagaggtcga
     1141 gctccttgcc gagcctgcgg ttgtcgcgtt tctcggcctc ctccagcatc ttgaggtagg
     1201 cctcgagctc ggccttgagc gagaagccca ggccgtagat gcgggtgagc atggggcgct
     1261 tctcgtcgcc gcgccagtag gccccggcca cggtcttgag cttgaaggcg tcgggcctga
     1321 tctcccgggt gctcgcgacg tgggggccgc ggcagagatc gacaaagccg tcctgctcgt
     1381 aggtgctgat ggtcccgtcc tcgagggcct cgatgagttc gaccttgaag ggctggtcgg
     1441 cgaagagcct gcgggcctcg tccttcggga tctcgcggcg gacgaactcg tggccgccgg
     1501 cgacgatgcg ccgcatctcc ttctcgatgg cggccaggtc ctcgttctgg atgggggcgg
     1561 ggagctggaa gtcgtagtag aagccgttct cgatggccgg gcctatgccg agcttggtgc
     1621 ccgggtagag cttgaggacg gcctcggcca ggatgtggct cagcgaatgc ctgatggtct
     1681 cgacgggagc tgacattggg tactcctttg gcgacgggtc ggggacccgg gccccggaaa
     1741 acgagaaggc ccccgggcag gcttcgtcct gcacggaaag gccttcgcgg cctttgcgta
     1801 aggacgaatt gcgcggaggc ctgacagacc cccaccggcc aacgctgggc cggcgccggt
     1861 cctgggagga ccggttcgcg aaatccgcgg taccacctta cttcggtccc cccgacgggg
     1921 accgcgctcg tcgccgttta cgccgaccta cggccccgga tactgcctgg ggcgcccatc
     1981 gcccttgggg ttcaccggga cggctcagga ggggttttcg ccgcgttcgc gccgccggga
     2041 ctctcaatcg cggtcccggc tccctgtggc ggagcatcgc ggctactcgt ctccatcgca
     2101 gccttcgggg gcgattatcc gattcgcggc ccggccttgt caatcggccc cggcctcggc
     2161 agcgccccgc ccctcggcct cggcctcaag gccgccgcga tccagcaggg cggcaagggc
     2221 ctcgacgacc cggccctcga actggctccc cttattccgc gcgatctcgg ccagggcctc
     2281 ggccgccgtc atggccccgc ggtaggccct ggacgaggtc atggcgtcga aggcgtcggc
     2341 cacgctcagg atcctcgaca gcatggggat gtcggcggcg gcgcagccca gatagccccg
     2401 gccgtcgagc cgctcatggt gcttcaccgc cacgtcgatg acccggccca ggtcggagaa
     2461 gggcgagagg atcctcgcgc ccttctccgg gtgcttcttt atgagctcgt actcctcgct
     2521 cgagaggggc ccggccttgt tcaggatgct ctcagggatc ccgatcttgc cgatgtcgtg
     2581 gaggctggcc gcgaccgaga ggacgtgcct gtcctcctcg ccaaggccga ggtcggcggc
     2641 gaggagccgc gaatagcgct cgacgcgccg cgagtggccc tgggtgtaat ggtccttctc
     2701 ctcgagggcg gtcgcgaggg agtcgaggat ctggtagaac tgctcctcga gcttggccgt
     2761 gcgctcccgc acgcgcagct cgaggtcctc gttggaggcc cgcagttccg cggccagctc
     2821 ctcgagccgt ttgcgcatct cctccctcgt gacgaagacg cggatcttcg agaccaggac
     2881 ctcggcgttg aagggcttgc ccacatagtc gtcggccccg ctccggtagc cgtcgagccg
     2941 catggaggtc tcggtcctcg ccgtcaggag gatcacgggc agctcggccg tcgcgggatc
     3001 ggccttcagc tcctcgcaga gcctgtagcc gtccatccgg ggcatcatca cgtcggagac
     3061 cacgatgtca ggagccctca gcctggcggc ctcgaggccc tcgatcccgt ccgaggccgt
     3121 ctcgacccgg aaatccctgg ccagtatgga gccgaggtat tcgcgcatct cggggttgtc
     3181 ctcgacgacg aggaccctcg cgcgcccgtc ctctgagggg cccatgggag cggggcccgc
     3241 ggcccccgcg gcggctgcat cagcccccgc ccccgcggca tcagcctccg agcccgcggc
     3301 ggcggccccc gcgcccgcgg cggcggcctc ggtcgcagcg ccaccgccct catgccgggc
     3361 cgggaccagg tcggagagca tcgaggcgcg cacgctgcgg atgtcctcgg cctgctcttc
     3421 ggccgagacc tcgccgatgg gcatgcggag gaggaagctt gagccctggc cgggtgcgct
     3481 cgtgaccgag atcgagccgc catggatcgc gacgatgtcc ttggtcaggg agagcccgat
     3541 gccggttccc tcgtagttgc gcgaggagga ggggtcgacc tggttgaagc gctcgaagat
     3601 cgactcgagt ttgtcggcgg cgatcccgat gccgctgtcg cgcaccgaga ggaggaagtc
     3661 cccgccctcg caggagagcg agaccgcgat gcttccgccc cggggcgtga acttgagggc
     3721 gttcgagagg aggttgtaga aggccttctc gaagaggtgg cggtcgaggc tcacgaaaag
     3781 gcaggggccg caggaggacc tgaaccccag ctcgaggccg cgggcctcgg cggccgattg
     3841 gaccgtggag aggtagtact cgagggccgc gacgaggtcg gtcttctggg ggcagagggc
     3901 catcttcccc gcctcgagct tagcgaagtc gaggaggcca ttg
//
LOCUS       DASSQV010000185         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1413217, whole genome shotgun sequence.
ACCESSION   DASSQV010000185 DASSQV010000000
VERSION     DASSQV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413217"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            415..1686
                     /locus_tag="VFL04_03210"
     CDS             415..1686
                     /locus_tag="VFL04_03210"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET7838744.1"
                     /translation="MKKKIALAYSGGLDTTVIVPWLKENYDCEVVAVCGDVGQECDWA
                     SLERKAISSGASKLIVKDLKAEFVTGYLWPLIKSGALYEGRYLLGTSAARPLLAKALA
                     ELALAEGCSAVAHGCTGKGNDQVRFELGVKAFAPEMEVIAPWRIWDISSREQEIEYLE
                     KRHIPVPMKKSDSYSRDDNLWHISHEGLDLEDPANEPRLGGEMLKMCVAPERAPDQSE
                     YLTVEFASGVPVSVDGVGMDGPALVKSLNARAGRHGVGILDMVENRVVGMKSRGVYET
                     PGGTVLMEAHAKLEQLCLDRKTFSFKQTVGQRFAELLYEGEWFSPLMTALNAFIDSTQ
                     KLVTGSVKLKLYKGSVTGVGASSPYSLYDASLASFTTGPLFSHKDSEGFINLFGLPTK
                     VRARLEARIKASGSAKPQTAKGAPIDVPAGD"
     gene            1696..>2332
                     /locus_tag="VFL04_03215"
     CDS             1696..>2332
                     /locus_tag="VFL04_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET7838745.1"
                     /translation="MAKLWETKAGPSVDPYIELFLSSTAVDARLLGEDLQGSAAHAEM
                     LGRRGIIDAETAAALAGGLAELAAEAASGSLAVDPEAEDVHSFVEAELTRRLGDPGRS
                     LHAGRSRNDQVALDLRLWLRRASKELRLALLDALGALCALAKGGTSLIMPGYTHLQRA
                     QAVTFAHQLLAWCAALERDYGRFSDAAARADESPLGSCALAGTGLPIDREAS"
BASE COUNT          393 a          856 c          741 g          342 t
ORIGIN      
        1 caagcggccc gcccttcgcc gtcttgccgc ggacctgccc gccgcgcccg actcttgcct
       61 gtccatcccc atgcccagcc gcggcgctct gcgtgtctcg tatatgcaca aaacggggca
      121 atattgggca aaagacggat aaatatgcaa aaaaccgccc atctcctgca taaaacgctt
      181 gacgctcggg gccctccctc cctatcatca aaccacgtca tcgccaagga ggtagcccag
      241 gtgtccccaa cgcatcagga ggccctcgcc tgccctaacg acagatcgcc ttcccccatc
      301 cgcccttttc ccacccgcct ccgtcgtcgc tgacattccc cccgcacagc agcagttccc
      361 cgttcggccg cccgcccagg cggtccggaa gccattccca caaaggagcg aaccatgaag
      421 aagaagatag ccctcgcgta ttcgggcggc ctcgacacga ccgtcatcgt cccctggctc
      481 aaggagaact acgactgcga ggtggtggcc gtctgcggag acgtgggcca ggaatgcgac
      541 tgggcctcgc tcgagaggaa ggccatctcc tcgggcgcct cgaagctgat cgtcaaggac
      601 ctgaaggccg agtttgtcac gggttacctc tggcccctca tcaagtcggg cgccctctat
      661 gaggggcgct acctcctcgg cacctcggcc gcgcggcccc tcctcgccaa ggccctcgcc
      721 gagctcgccc tcgccgaggg ctgcagcgcc gtcgcccatg gctgcacggg caagggcaac
      781 gaccaggtgc gcttcgagct cggcgtcaag gccttcgcgc ccgagatgga ggtcatagcg
      841 ccctggagga tctgggacat ctcgagccgg gagcaggaga tcgagtacct cgagaagcgc
      901 cacatccccg tgccgatgaa gaagtcggac tcctactccc gggacgacaa cctctggcac
      961 atcagccacg agggcctcga cctcgaggac ccggccaatg agccgaggct cggcggcgag
     1021 atgctcaaga tgtgcgtggc ccccgagagg gcccccgacc agagcgagta cctcacggtc
     1081 gagttcgcct cgggcgtccc ggtctcggtg gacggggtgg gcatggacgg ccccgccctt
     1141 gtgaaatcgc tcaacgcgcg cgcgggcagg cacggggtgg gcatcctcga catggtggag
     1201 aaccgcgtgg tcggcatgaa gtcccggggg gtctacgaga cccccggcgg caccgtcctc
     1261 atggaggccc acgccaagct cgagcagctc tgcctcgacc gcaagacctt ctccttcaag
     1321 cagaccgtgg gccagcgctt cgccgagctg ttgtacgagg gcgagtggtt cagccccctc
     1381 atgacggcgc tcaacgcctt catcgactcg acccagaagc tcgtgacggg ctcggtcaag
     1441 ctgaagctct acaagggatc ggtgaccggg gtgggcgcga gctcccccta ttccctctac
     1501 gacgcctcgc tcgcgagctt cacgacgggg cccctcttct cgcacaagga ctccgagggc
     1561 ttcatcaacc tcttcggcct gcccaccaag gtcagggcga ggctcgaggc gaggatcaag
     1621 gcgagcggca gcgcgaagcc gcagaccgcc aagggcgcgc cgatcgacgt gcccgcgggc
     1681 gactaggggg cagccgtggc gaagctctgg gaaaccaagg ccgggccctc ggtcgatccc
     1741 tacatcgagc tcttcctctc ctcgacggct gtcgacgccc ggctcctcgg cgaggatctc
     1801 cagggatcgg ctgcccatgc tgagatgctc gggaggcggg gcatcataga cgccgaaacg
     1861 gcggccgccc tcgcgggcgg cctcgccgag ctcgccgccg aggccgcctc ggggagcctg
     1921 gccgtggatc ccgaggccga ggacgtccac tccttcgtcg aggccgagct gacccgccgc
     1981 ctcggggacc ccgggcgcag cctccacgcg ggccgcagcc gcaacgacca ggtcgccctc
     2041 gacctgaggc tctggctcag gcgggcctcg aaggagctca ggctggccct gctcgacgcg
     2101 ttgggcgccc tctgcgccct cgccaagggc gggacctcgc tgatcatgcc gggctacacg
     2161 cacctgcaga gggcccaggc cgtcaccttc gcccaccagc tcctggcctg gtgcgccgcc
     2221 ctcgagcggg actacggccg cttctccgac gcggcagccc gcgccgacga aagccccctg
     2281 ggctcctgcg ccctcgcggg cacggggctg ccgatagaca gggaggccag cg
//
LOCUS       DASSQV010000186         4235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_294741, whole genome shotgun sequence.
ACCESSION   DASSQV010000186 DASSQV010000000
VERSION     DASSQV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294741"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFL04_03220"
     CDS             <1..942
                     /locus_tag="VFL04_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET7838746.1"
                     /translation="GERRYREAAVAHLPDFRARLDGRRATETHDLGFLYTLSCVPAWR
                     LEGDEGARAAALKAADLLRVRYHEKAGVIQAWGDLNDPEQRGRIIIDCAMNLPLLHWA
                     SEETRNPYYREAAVSHLARANASLIRSDWSSYHTFYFDTATGEALRGITHQGYADDSC
                     WARGQAWAILGNALCYRYVRDPAYLETARGVAHYFLNRLPDDLIAYWDLVFTEGTAER
                     DSSAVAIAACGLLELSGALPHLDPLRRSYENAALRLIAALAERCTAAAESRSNAILLH
                     GVYNKPKLVGVDEAVIWGDYFFVEALVRALVGWKPYW"
     gene            954..2819
                     /locus_tag="VFL04_03225"
     CDS             954..2819
                     /locus_tag="VFL04_03225"
                     /inference="COORDINATES: protein motif:HMM:NF021534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HET7838747.1"
                     /translation="MRATTTQEALARNPLRDRAELEHSLLDLLAPLEGRYAAGNAGLD
                     LGAAGAIFSPRVARLEGWSRVLWGIAPLTAGGGSYPAAAKHLEGLRRGPDPRDPAYWG
                     EPGDSDQRLVEMAAIALCLLIAREAYWDPLSKAEKARLHAWLSTIQTRRLPANNWHFF
                     RILVCSAFRRLDLPLDEAAEEESFALIETMYRGEGWYIDGCNGSYDFYNPFGFHFYGL
                     VYARLMGDLFPERAARYVERARLFAPQFLGLFRPDGSNVGFGRSLGYRFAAVSFFSAC
                     AFAGVEALPWPVMKGLVLRSLRWWFSRPILDSGGLLTVGYAYPNLVMAEQYNSPGSPY
                     WALKAYLPLALAPEHPFWRAEEAPLPEQAAVSRLSPTHLLINRGPEDAQLLNPGAYPS
                     WESVQAAAKYCKFAYSARFGFSVSRGSYALEQTGCDSSLVLSEGEGYWRERRETVDQD
                     SGPNWTRGTWRPWPDVEIVTVLVALGSSHLRLHRIRTTRRLEAVEGGFSIPRFRGAEP
                     AAEPRLGLGPGTAMVAFPWAGSRIDGLATAKGAAPRGGEALCLSPNLNILEPSGIVPL
                     LRGLVAPGETLWACVVRAGDEGPCLSAALPRLSDLGRGRYAVLDGAGGLLAEFEV"
     gene            2968..3945
                     /locus_tag="VFL04_03230"
     CDS             2968..3945
                     /locus_tag="VFL04_03230"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET7838748.1"
                     /translation="MSVRIASVGVHIPQRRMSNEELASMIDTTDEWIRSHTGIGNRHI
                     AADGSLTSDLAADAARSALARAGVGATDLDFILVATATPDYFGFPSTACVIQEKLGAK
                     GCAAFDISAGCTGFIYALEIAASMLEARRGAHALVIGAETLTRVTNWADRSTCVLLGD
                     GAGAAVLSRTDENGRGILDAILGADGSGAKDLRLVQAERTTTFRPAAAMVPYLEMNGK
                     SVYNFAVKTIVSLVEGLLAKGSHAMGDIDWVVPHQANARILQAAAKRLGLPESRFYMN
                     MEEYANTSSASIPIALEEMDRKGLLKQGALVMLLGFGAGLTYGGALLRW"
     gene            complement(3950..>4235)
                     /locus_tag="VFL04_03235"
     CDS             complement(3950..>4235)
                     /locus_tag="VFL04_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7838749.1"
                     /translation="EDARTAVLCASDIFALELLNQFRGRGLRVPRDVGLAGFDSIDAL
                     RFISPRITTVEYPIQRMGELAFALLAEGPKGGADLPQIELEPEILWGESV"
BASE COUNT          621 a         1597 c         1372 g          645 t
ORIGIN      
        1 ggcgaaaggc gctaccgcga ggccgcggtc gcccatctcc ccgatttccg cgccaggctg
       61 gacgggcgca gggcgaccga gacccacgac ctcggtttcc tctatacgct ctcctgcgtc
      121 cccgcctggc ggctcgaggg cgacgagggg gcgagggccg cggccctcaa ggccgccgac
      181 ctcctcaggg tccgatacca cgagaaggcc ggggtcatcc aggcctgggg cgacctcaac
      241 gatcccgagc agcggggtcg gatcatcatc gactgcgcga tgaacctgcc cctcctccac
      301 tgggcctcgg aggagacgag gaacccctac taccgggagg ccgcggtgag ccatctggct
      361 cgggccaacg cgagcctcat ccgcagcgac tggtcgagct accacacctt ctacttcgac
      421 accgcgacgg gcgaggcgct gcggggcatc acccaccagg gctacgccga cgactcctgc
      481 tgggcccgcg gccaggcctg ggccatcctc ggcaacgccc tctgctaccg ctatgtccgc
      541 gatcccgcct acctcgagac ggcgcgcggg gtcgcccatt acttcctcaa ccgcctgccc
      601 gatgacctca tcgcctactg ggacctcgtc ttcactgagg ggacagcgga gcgcgacagc
      661 tcggccgtgg ccatcgcggc ctgcggcctc ctcgagcttt cgggcgccct cccccatctc
      721 gatcccctga ggcggagcta cgagaacgcc gcgctccggc tcatcgcggc cctcgctgaa
      781 cgctgcacag ccgccgccga gagccggtcg aacgcgatcc tcctccacgg cgtctacaac
      841 aagcccaagc tcgtgggcgt ggacgaggcg gtcatctggg gcgactactt cttcgtcgag
      901 gccctggtgc gcgccctcgt cggctggaag ccctactggt aaggatggag cagatgcgag
      961 ccacgacgac ccaggaggcc ctcgcccgca accccctcag ggacagggcc gagctcgagc
     1021 attcccttct cgacctgctg gcgccgctcg agggccgcta cgccgcgggc aacgcgggcc
     1081 tcgacctggg ggcggccggg gccatcttct ccccccgggt cgcccggctc gagggctgga
     1141 gccgcgtgct ctggggcatc gcccccctga ccgccggcgg cggctcctac cccgcggccg
     1201 cgaagcacct cgagggcctg cggcgcggcc ccgatccgcg cgatcccgcc tactggggcg
     1261 agccggggga cagcgaccag aggctcgtcg agatggccgc gatcgccctc tgcctcctca
     1321 tcgcgcggga ggcgtactgg gacccgctct ccaaggccga gaaggcgagg ctccacgcct
     1381 ggctctcgac gatccagaca aggcgtctgc cggctaacaa ctggcacttc ttccgcatcc
     1441 tcgtctgctc ggccttccgg cgcctcgacc tgcccctcga cgaggccgcc gaggaggagt
     1501 ccttcgcgct gatcgagacc atgtaccgcg gcgagggctg gtacatcgac ggctgcaacg
     1561 gcagctacga cttctacaac cccttcggct tccatttcta cggcctggtc tacgcgcggc
     1621 tcatgggcga tctctttccg gagcgggccg ctcgctatgt cgagcgggcc cggctcttcg
     1681 ccccgcagtt cctcgggctc ttccgccccg acggctcgaa cgtcggcttc ggaaggagcc
     1741 tcggctaccg cttcgccgcg gtctccttct tctcggcctg cgccttcgcc ggcgtcgagg
     1801 ccctgccctg gccggtcatg aagggcctcg tcctcaggag cctcagatgg tggttctcgc
     1861 ggcccatcct ggactcgggc ggcctcctca cggtcggcta cgcctatccg aacctcgtga
     1921 tggccgagca atacaattcg ccgggctcac cctactgggc cctcaaggcc tacctccccc
     1981 tcgccctcgc tcccgagcac cctttctggc gggccgagga ggcccccctc cccgagcagg
     2041 ccgccgtgag ccggctgtcg ccgacccatc tcctcatcaa caggggacct gaggatgccc
     2101 agctcctcaa tccaggcgcc tacccctcct gggagtccgt ccaggcggcc gccaaatact
     2161 gcaagttcgc ctattcggcg cgctttggct tctcagtctc acgagggtcc tacgcccttg
     2221 agcagacggg ctgcgactcc tcccttgtgc tctccgaggg cgaaggctac tggcgggagc
     2281 gaagggagac cgtcgaccag gactcgggcc cgaactggac acgggggacc tggaggccct
     2341 ggcccgacgt cgagatcgtc acggtcctcg tggctctcgg ctcctcccac ctccgccttc
     2401 accgaatccg cacgaccagg aggctcgagg ccgtcgaggg aggcttctcg atccctcgct
     2461 tccgcggcgc agagcccgcg gccgagccga ggctcgggct cggccccggg acggccatgg
     2521 tcgccttccc ctgggccggc tcccggatag acgggctcgc gaccgccaag ggcgcggccc
     2581 cgcgcggcgg tgaggccctc tgcctctcgc ccaacctcaa catcctggag cccagcggga
     2641 tcgtccccct cctccgcggc ctggtcgcgc cgggcgagac cctctgggcc tgcgtggtgc
     2701 gcgcggggga cgaggggccc tgcctctccg ccgccctgcc caggctctcg gatctgggac
     2761 ggggacgtta tgcggtcctg gacggggccg gaggcctcct ggccgagttt gaggtctagg
     2821 tccgggccac cccgcgcaac ttcctgtcag gcccggccgc ggaggcccga ccgcggagat
     2881 ccggccgacc gaggactttc tcgatcttcc ctgcaaccct tccccgctcg gaatgtgtct
     2941 aaaggccaaa gacgaggggg aggaatcatg agcgttcgca tagcttccgt cggggtccac
     3001 ataccgcagc gcaggatgag caacgaggag ctcgcctcga tgatcgacac caccgacgaa
     3061 tggatcaggt cccacacggg catcggcaac cgccacatcg ccgccgacgg ctcgctgacg
     3121 agcgacctcg ccgccgacgc ggcccgctcg gccctggcca gggcgggcgt cggcgcgacc
     3181 gacctcgatt tcatcctcgt ggccacggcc acgcccgact acttcggctt cccctcgact
     3241 gcctgcgtta tccaggagaa gctcggcgcc aagggctgcg ccgccttcga catcagcgcc
     3301 ggctgcaccg gcttcatcta cgccctcgag atcgccgcga gcatgctcga ggcgaggagg
     3361 ggcgcccatg ccctcgtgat cggcgccgag accctcaccc gcgtcaccaa ctgggcagac
     3421 cgctccacct gcgtcctgtt gggcgacggg gccggggcgg ccgtgctctc ccgcaccgac
     3481 gagaacggcc ggggcatcct ggacgccatc ctgggcgccg acggctcggg ggcgaaggac
     3541 ctgcgtctgg tccaggccga gcgcacgacg accttcaggc ccgccgcggc catggtcccc
     3601 tacctcgaga tgaacgggaa gagcgtctac aacttcgcgg tgaagaccat cgtctccctc
     3661 gtcgagggcc tcctcgccaa gggctcccac gcgatgggcg acatcgactg ggtggtcccg
     3721 caccaggcga acgcccgcat cctgcaggcc gcggccaagc gcctgggcct gccggagagc
     3781 aggttctaca tgaacatgga ggagtacgcg aacacctcct cggcgagcat ccccatcgcc
     3841 ctcgaggaga tggaccgcaa gggcctcctg aaacaggggg ccctggtcat gctcctcggc
     3901 ttcggcgccg gcctgaccta cggcggggcc ctgctgcgct ggtagggccc tagaccgact
     3961 cgccccagag gatctcgggc tcgagctcga tctggggcag atcggcgccg cccttcggcc
     4021 cctcggcgag cagggcgaag gcgagttccc ccatcctctg gatcgggtac tccacggtgg
     4081 tgatcctcgg gctgatgaac ctgagggcgt cgatcgagtc gaagcccgca aggcccacat
     4141 cccggggcac gcggaggcct cggccacgga actggttcag gagctcgagg gcgaagatgt
     4201 cgctggcgca gaggacggcc gttcgggcat cctcg
//
LOCUS       DASSQV010000187         4915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2160959, whole genome shotgun sequence.
ACCESSION   DASSQV010000187 DASSQV010000000
VERSION     DASSQV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2160959"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1534)
                     /locus_tag="VFL04_03240"
     CDS             complement(287..1534)
                     /locus_tag="VFL04_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838750.1"
                     /translation="MSSYLRSSLAELSVLPYPNGVRLVVKRQTGRPMAAARVTLAGGG
                     DSEALALYLAALGGGPSPAGLGPELDEQGATLGMDLDSTGDAAISLAAPAEALGGLLA
                     AIGRNLLSPVFRQADFATATAALREARRVEAADPGRRSAGLLRDEMEGRRAAAFPAPG
                     IQAIREAWLLSFSPERLSVVVVGDLEAASLASSLASGFGAMTRGATEAGTATRPSPGF
                     QTLRLAQAQSSDAGRLSLRAEPRTPAIASRDYAALAVALTMLDGLLGPGTRTALPGPL
                     TSRPSILIEAAASPRDAMAELDAAATLIASGYCGSPQGREAGAVPIGAALEAYRSRTL
                     ARLYAGSAAGPELAARIALGLETAGDAGAFFRAADALASLSPEDVSRAARVYLGEDGL
                     AWLALGDPRLIKALEALGFTETP"
     gene            complement(1981..3468)
                     /locus_tag="VFL04_03245"
     CDS             complement(1981..3468)
                     /locus_tag="VFL04_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET7838751.1"
                     /translation="MRTIHFSRRSFAMKRALLAALCLLILSLAAQSEEGSLPNLSRFR
                     LSNGLEVFAYRDSALPLARVQISFRAGAMAQGPETAGLFRLYERMLFHGEPALPGDSP
                     LRSALAGLGLAGAEGGTTAESVAWWITVPSARTDEALALWSSLCREPAFDSTELEARK
                     AECLRAIAEGAKPDTIYEAAITRRLFAKYPWRRDPAGSEKAINSASLDSLRTLQRTWF
                     VPNNAALMVAGDIDPEAVRASAERLFGAWKPGDDPWKKPLPPQPKPGVPRPTWIVYPD
                     PSVPEGVASVEARYRGPDALADIPATYGGDLWSALVSSPEGKFKTAVMKTVPKLFGPE
                     SIQASYATQRDGAWISISSFFYVDPALPAVDRARLFKERVRGFEITAMKTDPSYFSAK
                     DYEDARRRLLVDRDLGLDTPDGVLDALAFWWSSSSVDYFLGYPAAMAKAGPKELGAFL
                     DTYIMRNLEVVALRMNPADYEREKKSFAGSGFELVTPSNAFWWQK"
     gene            3600..4706
                     /locus_tag="VFL04_03250"
     CDS             3600..4706
                     /locus_tag="VFL04_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET7838752.1"
                     /translation="MLPFPPSHAQFSRRADRSLGWARLEARQARALVGLLQSGGRAEI
                     GLPAELLPLRTAPRRLGPGRILGMAAALILGLQATYMVATALLIAVYRHVDPPATVLM
                     AYRKWGYGWSLEAPRRLSLHRIPVYVRSMLVSVEDGKFFEHHGLDFEAFRNAREINAR
                     FGKPLYGGSTLTMQVARTLFLVPEKSYLRKYLEIVAALELELFLPKDRILELYFDYAE
                     WGKGIFGMEAAARHWYGVGVSGLSRDEAARLIALLSSPIRYRPDNLERNGILRERYAY
                     LQRRYVAMAAPPASDVSLVPAPESPPNPPPPSVEPDAAVADAPADVAAPGAAAQTQGA
                     GAALAPAAPAATPGAPGAVASTPGAPAPKPAAKP"
BASE COUNT          665 a         1706 c         1871 g          673 t
ORIGIN      
        1 cccctgggcg cgccctgggg gtggaggttg aggtggtcgg cgagctggct gtagccgttc
       61 ttgagcgcgt tgtgggccat ggggatctcc ttgccgggcg gttcaaggcc cacgatagcc
      121 gaagaacggg gcgggcgcaa cgcggaatcc gcgggcccgg gcgcataccc caggcgggcg
      181 cccggtgtgg gcacggggcc cagcgcggct cgcgaccaaa ggggcctcct accgcggccc
      241 cttgccagac cgaaaggggc tttgtaccga aacccctcgc gggttcctaa ggggtctctg
      301 tgaagcccag ggcctcgagg gccttgatga ggcgggggtc gccgagggcg agccaggcga
      361 ggccgtcctc accgaggtag acgcgcgcgg cccggctcac gtcctcgggc gagagcgagg
      421 cgagggcgtc ggccgcgcgg aagaaggcgc cggcgtcccc cgcggtctcg aggccgaggg
      481 cgatgcgcgc ggcgagctcg ggccccgcgg ccgagccggc gtagagccga gcgagggtcc
      541 tcgacctgta ggcctcgagg gcggcgccga tggggacagc gccagcctcc cgcccctgcg
      601 gcgacccgca atagcccgag gcgatgaggg tcgccgcggc gtcgagttcg gccatcgcgt
      661 cgcgcgggga ggccgcggcc tcgatcagga tcgagggccg gctcgtgagg gggccgggca
      721 gggctgtccg cgtccccggg ccgaggaggc cgtccagcat ggtgagggcc acggcgaggg
      781 cggcgtagtc cctgctggcg atggccggcg tccttggctc tgccctcagg ctgagcctgc
      841 ccgcatcgga gctctgggcc tgggccagac gcagggtctg gaacccgggg ctcggcctcg
      901 tggccgtccc ggcctcggtg gccccccgcg tcatggcccc gaagcccgat gcgagggagg
      961 aggcgaggga agccgcctcg aggtcgccca cgaccacgac cgagaggcgc tcgggggaga
     1021 aggagaggag ccaggcctcg cggatggcct ggatcccggg ggctggaaag gcggccgccc
     1081 gccgcccctc catttcgtcc cgaagtaggc cggccgacct gcggcccggg tctgcggcct
     1141 cgacccgtct cgcctcgcgg agagccgcgg tcgcggtagc gaagtcggcc tggcggaaga
     1201 cgggagagag gaggttgcgc ccgatggcgg cgaggaggcc tccgagggcc tcggccgggg
     1261 cggcgaggct gatggcggcg tcccccgtcg agtcgaggtc catgcccagg gtggcgccct
     1321 gctcgtcgag ttcggggccg agcccagccg ggctcgggcc gccgcctagg gcggcgaggt
     1381 agagggccag ggcctcgctg tcgccgcctc cggccaaggt gacgcgcgcg gccgccatgg
     1441 gcctgccggt ctggcgcttc acgaccagac ggacgccgtt gggatagggg aggacggaga
     1501 gctcggcgag gcttgacctc agataggagc tcatcggggg agggatcggc ggagccggga
     1561 cggggcgctt gggggacacg agggcgaggt ctggcttcgc cgccggctcc ttccttggcg
     1621 ccgaggccgg accgggctgg gagccgggca aggagccggg caaggagccg ggcaaggagc
     1681 ccgccggggc ggggagggcc gaccctgccg cccccgcgcc cgagggagtc tggccggcag
     1741 tctgggggct cggcgcggcg accgccaggg cggggccggc tccgcccgcg gcggcggggc
     1801 tcccgggcgc cgctgccgtc gtcccccttt gggaggcgtg ggcctggccg cccgtgacgg
     1861 ccgggacggg aaggggccct gccggggggg cttgtcttgt cgcgcaggcg ccggtagcga
     1921 gcgcggccgc gagggcgagg agccaggcgg cgccgccact cccgcgtggg gcgcccgtcc
     1981 tcacttctgc caccagaagg cgttggacgg ggtgaccagc tcgaagcccg agccggcgaa
     2041 gctctttttc tcccgctcgt agtcggcggg gttcatccgg agcgcgacga cttcgaggtt
     2101 gcgcatgatg taggtgtcga ggaaggcccc gagctccttc ggccccgcct tcgccatcgc
     2161 cgcggggtag ccgaggaaat agtcgaccga ggagctgctc caccagaagg cgagggcgtc
     2221 caggaccccg tcgggcgtgt cgaggccgag gtccctgtcg acgaggagcc gtctgcgcgc
     2281 gtcctcgtag tccttcgcgc tgaaatagga gggatcggtc ttcatcgcgg tgatctcgaa
     2341 gcccctcacc cgctccttga agagcctggc cctgtccacg gccggaaggg cggggtcgac
     2401 gtagaagaag gacgagatcg agatccaggc cccgtcgcgc tgggtggcgt agctcgcctg
     2461 gatggactcg gggccgaaga gcttgggcac cgtcttcatg accgccgtct tgaacttgcc
     2521 ctcggggctc gagacgaggg ccgaccagag gtcgccgccg taggtggcgg ggatgtcggc
     2581 gagggcgtcg gggccgcggt agcgggcctc gaccgaggcc accccctcgg ggacagaggg
     2641 atcggggtag acgatccagg tgggcctcgg gacccccggc ttcggctggg gcggcagggg
     2701 ctttttccag gggtcgtcgc cgggcttcca tgcgccgaag agccgctcgg ccgaggcgcg
     2761 gacggcctcg ggatcgatgt cgcccgccac catgagggcg gcgttgttcg gcacgaacca
     2821 ggtcctctgg agggtcctga gggagtcgag gctggctgaa ttgatggcct tctcgctgcc
     2881 agcggggtcg cggcgccagg ggtacttggc gaagagcctg cgcgtgattg cggcctcgta
     2941 tatcgtgtcg ggcttcgccc cttcggcgat cgcgcgcaga cactcggcct tcctcgcctc
     3001 gagctccgtg gagtcgaagg ctggctccct gcagagggat gaccagaggg cgagggcctc
     3061 gtcggtcctc gccgagggca cggtgatcca ccaggccacg ctctcggccg tggtcccgcc
     3121 ctcggctcct gcgagcccga gcccggcgag ggcggagcgc agtggggaat cgcccggcag
     3181 ggcgggctcg ccgtggaaga gcatgcgctc gtagaggcgg aagaggccgg cagtctcggg
     3241 gccctgggcc atcgcccccg ccctgaagct tatctggacc ctggccaggg gaagggccga
     3301 gtcgcggtag gcgaagacct cgaggccgtt ggagagccgg aaacggctca ggttcggcag
     3361 gctcccctcc tcggactggg ctgcgaggga gaggatgagc aggcagagag cggcgagaag
     3421 cgcgcgcttc atggcgaagg acctcctgga gaaatggatc gtccgcatcg gcttcagcct
     3481 tcgtgcatcc cgccgcgcaa gcggcctgag tctagcaagg cccggaaagc ggtgtcaaga
     3541 aaacggatct tgcattcttg tgtcttttcg cggggccgtg ctatcgtgcg gggcgaatca
     3601 tgcttccatt ccccccctca catgcgcagt tctcgcgaag ggccgaccgc agcctcggct
     3661 gggccaggct cgaggcgagg caggcgcgcg ccctcgtcgg cctcctccag tctgggggca
     3721 gggccgagat cggcctgccc gccgagctcc tccccctccg gaccgcgccg agacggctcg
     3781 ggcccggacg catcctcggc atggccgccg ccctcatcct gggcctccag gccacctaca
     3841 tggtagcgac agccctcctc atcgccgtct accgccacgt cgacccgccc gccaccgtcc
     3901 tcatggccta ccgcaagtgg ggctatggct ggagcctcga ggcgccgagg aggctgagcc
     3961 tccaccggat ccccgtctat gtccgctcga tgctcgtctc ggtcgaggac ggcaagttct
     4021 tcgagcacca tggcctcgat ttcgaggcct tccgcaacgc ccgcgagatc aacgcgcgct
     4081 tcggcaagcc cctctacggc gggtccaccc tgacgatgca ggtggccagg accctcttcc
     4141 tcgtccccga gaagagctat ctgcgcaagt acctcgagat cgtggcggcc ctcgagctcg
     4201 agctcttcct gcccaaggac aggatcctcg agctctactt cgactacgcg gaatggggca
     4261 agggcatctt cgggatggag gcggccgccc ggcactggta cggggtgggg gtctccgggc
     4321 tctcgaggga cgaggccgcc cggctcatcg ccctgctctc ctccccgata cgctacaggc
     4381 ccgacaacct cgagaggaac ggcatcctcc gcgagcgcta cgcctacctg cagcggcgct
     4441 acgtcgccat ggccgcgccc ccggcgagcg acgtctccct ggtccccgcg cccgagagcc
     4501 cgccgaaccc cccgccgccc tcggtcgagc ccgacgccgc tgtcgcggac gcccccgccg
     4561 atgttgctgc gccgggcgcc gccgcgcaga cccagggcgc cggggccgct ttggcgccgg
     4621 ccgcccccgc tgcgacccct ggagccccgg gagccgtggc ctcgacccca ggagccccgg
     4681 ccccgaagcc tgcggcgaag ccctagatcc ctcgaagggc gcccgcgccc ctagatccct
     4741 cgaagggcgc ccgcgcccct agatccctcg gagggcgcgg cccagctcgc gatcgaaggc
     4801 gtcggcgaag gccttgagct cgcgggcgcc ccaggtcctc gccttcggcg actgcggcgc
     4861 gaggccgagg gccctgagct cctgggcccg gtcgcgcagc gagcggcgct ccctc
//
LOCUS       DASSQV010000188         3547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1910959, whole genome shotgun sequence.
ACCESSION   DASSQV010000188 DASSQV010000000
VERSION     DASSQV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910959"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VFL04_03255"
     CDS             <1..575
                     /locus_tag="VFL04_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7838753.1"
                     /translation="TYAVEIYRRWAEVHDVPEAEAQDFQDLLRISAMLHDIGKAGIPE
                     AILKKPGKLSPEEYEIVKRHVLLGACFFENPSSDWDALAREIALNHHERWDGTGYPGV
                     RDGVCYDSTRFGPGKKGREIPLSARIVAVADVYDALVSERPYKDPWEEDRALHYLRQE
                     SGKHFDPEIVDIFLEVHDLILAARRDGNAA"
     gene            635..1255
                     /locus_tag="VFL04_03260"
     CDS             635..1255
                     /locus_tag="VFL04_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7838754.1"
                     /translation="MITKAQGPDDSLALGEGADPALSSYYRLMRLKRLYRQGWLKRGL
                     PAELCESVAEHSFGTALLALLLAPSGGASRAEGASLDRSKATLMALVHELGEAYAGDI
                     TPVDGVPREEKTRLERESILRALEGHSDLTWFLDLWEEFERGESPEARFVRELDRLEM
                     GLQAALHRAEGHPGMEEFFASARRSVSSPALRSLLEDAIAASGPGS"
     gene            1270..1896
                     /locus_tag="VFL04_03265"
     CDS             1270..1896
                     /locus_tag="VFL04_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838755.1"
                     /translation="MGNRFVALIKSGAIESVEALKAEFKALAKLTHPDLAGPGSTGEE
                     FIRARREYESALRDFGRHRFGFSGAEPAGRAPSVLDRRTLYAGLAALLGRGFPKKARH
                     EKESKRYAYGKHLVMAQLGVWKDGRARLFEAFEAGLLELKRGDPRRCEAAMDLLRSVL
                     DYHTGGPELARAAIELDLQLWKKAGGPGPVAEFLELLVEDLSRGQALR"
     gene            1958..>3547
                     /gene="ettA"
                     /locus_tag="VFL04_03270"
     CDS             1958..>3547
                     /gene="ettA"
                     /locus_tag="VFL04_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET7838756.1"
                     /translation="MATVDDKRIIYTMDRVTKRHGTKVVLKDIRLSYYYGAKIGVIGL
                     NGSGKSSLLRILAGLDTEILGETHVEKGFTIGFLEQEPQLESGKTVKQVVSEGVQELV
                     DMLAEFEAIGEAYGDPDADFDKLAAKQARLQEKLDETDAWNLDARLDLAMDALRCPPA
                     DRVVDVLSGGERRRVALCRLLLRKPDILLLDEPTNHLDAETVAWLERHLREYEGTVIA
                     VTHDRYFLDNIAGWILELDRGEGIPWKGNYSSWLDQKKKQLEMEEKGDSDRAKTLARE
                     LEWIGMSPKGRHAKSKARIERYEKLLNDEGREKVKESKIVLPAGPRLGNVVIEAEGIA
                     KGYDGKLLYEGLSFVVPPGAIVGLIGPNGAGKTTLLRMITGEEKPDSGSIRLGDTVKL
                     AYADQGRARLDPEKSVWEQLSGGLDVIKLGKREVNSRAYCTWFNFSGTDQSKKVGVLS
                     GGERNRLNMAMMVQDCANVLLLDEPTNDLDVNTMRALEEALDEFAGVALVVSHDRWFL
                     DRVCTHILAFEGNSEAIWYDGN"
BASE COUNT          627 a         1247 c         1160 g          513 t
ORIGIN      
        1 gaacctacgc cgtggagatc tataggcgct gggccgaggt ccatgacgtg cccgaggccg
       61 aggcccagga tttccaggac ctgctgcgga tctcagccat gctccacgac atcggcaagg
      121 cgggcatccc cgaggccatc ctcaagaagc cggggaagct gagcccggag gagtacgaga
      181 tcgtcaagcg ccacgtcctc ctgggcgcct gcttcttcga gaacccgagt tcggactggg
      241 acgccctcgc ccgggagata gccctgaacc accatgagcg ctgggacggc acgggctatc
      301 ccggggtcag ggacggggtc tgctacgatt ccacgcgttt cggcccgggg aagaagggca
      361 gggagatccc cctctcggcc aggatcgtcg ccgtcgccga cgtgtatgat gccctcgtga
      421 gcgagcgccc ctacaaggat ccgtgggaag aggacagggc cctccactac ctgcgccaag
      481 agtcgggcaa gcacttcgat cccgagatcg tcgacatctt cctcgaggtc cacgacctga
      541 tcctggccgc ccgccgcgac ggcaacgcgg cctagccgga ggccgccggc cgggcgcgtc
      601 gccgcgcagg gcccgtccgt gatagagtcc cgccatgata acgaaggccc aggggcccga
      661 tgacagcctc gccctcggcg agggcgccga ccccgccctc tcgtcctact atcggctcat
      721 gaggctcaag cggctctacc gccagggctg gctcaagcgc gggctcccgg ccgagctctg
      781 cgagtccgtc gccgagcaca gcttcgggac agcccttctc gccctcctcc tcgctccctc
      841 tggtggcgcc tccagggcgg agggagcgag cctcgacagg tccaaggcca ccctcatggc
      901 cctcgtccat gagctgggcg aggcctacgc cggggacatc acccccgtgg acggcgttcc
      961 gcgcgaggag aagacccgac tcgagcgcga gtcgatcctg agggccctcg aggggcactc
     1021 cgacctcaca tggttcctcg acctttggga ggagttcgag cggggcgaga gccccgaggc
     1081 ccgcttcgtt cgggagctcg accgactgga gatgggcctc caggccgccc tccacagggc
     1141 cgagggccat cccggcatgg aggaattctt cgcgagcgcg cggcgctccg tgtcgagccc
     1201 cgccctccgc tccctcctcg aggacgccat cgccgcgtcc ggtcccggct cctgaaaccc
     1261 ttcccagtcg tgggcaaccg cttcgtcgcc ctcataaaga gcggggccat cgagtcggtc
     1321 gaggccctga aggccgagtt caaggccttg gccaagctga cccatcccga cctcgcgggg
     1381 ccgggatcga cgggcgagga gttcatcagg gcccggcgcg agtacgagtc cgcgttgcgc
     1441 gacttcggcc gccaccgctt cggcttttcg ggggcggagc ctgcggggag ggcgccctcg
     1501 gtcctcgatc ggcgaaccct ctatgcaggt ctggccgccc tcctcgggcg aggcttcccg
     1561 aagaaggccc gccacgagaa ggagtccaaa aggtacgcct acggcaagca cctggtcatg
     1621 gcccagctgg gcgtctggaa ggacgggcgc gcccggctct tcgaggcctt cgaggctggg
     1681 ctcctcgagc tgaagcgcgg cgatccgcgg cgctgcgagg ccgccatgga ccttctgcgc
     1741 tccgtcctcg actaccacac ggggggcccc gagctggcca gggctgccat cgagctcgac
     1801 ctccagctgt ggaagaaggc cggcggaccg ggcccggtgg cggagttcct tgagctcctt
     1861 gtcgaggacc tgtcccgagg ccaggccctc aggtagaccg gagctccggg cccctcatca
     1921 ctacacgagg atgccccttt gctgctatag tcggttcatg gcaacagtcg acgacaagag
     1981 gatcatctac accatggacc gtgtcaccaa acgccacggc accaaggtcg tgctcaagga
     2041 cataaggctt tcgtactact acggcgccaa gatcggcgtc atcggcctga acgggtctgg
     2101 aaagtcgagc ctcctccgca tactcgcggg gctcgacacc gagatcctcg gcgagaccca
     2161 cgtcgagaag ggcttcacga tcggcttcct cgagcaggag ccccagctcg agagcggcaa
     2221 gacggtcaag caggtcgtga gcgaaggggt ccaggagctc gtggacatgc tcgccgagtt
     2281 cgaggccatc ggcgaggcct acggggaccc cgacgccgac ttcgacaagc tggccgcgaa
     2341 acaggccagg ctccaggaga agctcgacga gaccgacgcc tggaacctcg acgcccgcct
     2401 cgatctggcg atggacgccc tccgctgccc cccggccgac agggtagtcg acgtgctctc
     2461 gggcggcgag cgccggcgcg tggccctctg ccgcctcctc ctgcgcaagc cggacatcct
     2521 cctcctcgac gagcccacca accacctcga cgccgagacc gtggcctggc tcgagcgcca
     2581 cctccgcgag tacgagggca cggtcatcgc cgtcacccac gaccgctatt tcctcgacaa
     2641 catcgcgggc tggatcctcg agctggaccg cggcgagggc atcccctgga agggcaacta
     2701 ctcgtcctgg ctcgaccaga agaagaagca gctcgagatg gaggagaagg gcgactcgga
     2761 ccgggccaag accctggccc gcgagctcga gtggatcggg atgagtccca agggcagaca
     2821 cgccaagtcc aaggcccgca tcgagcgcta cgaaaagctc ctcaacgacg agggccgtga
     2881 gaaggtcaag gagtcgaaga tcgtcctgcc cgccggaccc cgcctcggca acgtggtgat
     2941 cgaggccgag ggcatcgcca agggctatga cggcaagctc ctctacgagg gcctgagctt
     3001 cgtcgtgccg cccggcgcga tcgtcggcct catcggcccg aacggcgccg gcaaaacgac
     3061 cctccttcgc atgatcaccg gcgaggagaa gcccgactcg ggcagcatca ggcttggcga
     3121 cacggtcaag ctcgcctacg ccgaccaggg ccgcgcccgc ctcgaccccg agaaatcggt
     3181 ctgggagcag ctctccggcg gcctcgacgt catcaagctc ggcaagcgcg aggtgaactc
     3241 gcgcgcctac tgcacctggt tcaacttctc cggcaccgac cagtcgaaga aggtgggcgt
     3301 cctctcgggc ggggagcgca acaggctcaa catggccatg atggtccagg attgcgcgaa
     3361 cgtgctcctc ctggacgagc cgaccaacga cctcgacgtg aacacgatgc gcgccctcga
     3421 ggaggccctg gacgagttcg ccggcgtggc ccttgtcgtg agccacgacc gctggttcct
     3481 cgacagggtc tgcacccaca tcctcgcctt cgagggcaac agcgaggcca tctggtacga
     3541 cgggaac
//
LOCUS       DASSQV010000189         3133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_420552, whole genome shotgun sequence.
ACCESSION   DASSQV010000189 DASSQV010000000
VERSION     DASSQV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420552"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VFL04_03275"
     CDS             <1..1187
                     /locus_tag="VFL04_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET7838757.1"
                     /translation="KGVSLGLIATRLFTGLDSFTFMAIPFFILAGELMNSSGITKRLV
                     NFTSVFVGRLRGGLGHVSVVTSMFFGGVTGSAIADCSAVGTMMIPAMKEKGYDEEFAA
                     GLIASSAIMGPIIPPSIPFVVYALLSSTSVAALFLGGIVPGVMIAIGCMAVTYVIATR
                     RDFPRGERVRSLGQFLRALADGFIPLMMPFIILGGIIGGIFTATEASAVAVLYGLVVG
                     LIVYRDLPVSAIRTALAGTAKTTGVVFLVIACSNVFNWMLVVEQVPQAMASFVSGFIH
                     DRWAILLAINVILLIVGTFMEGTAAMIILVPILLKITAVYHIHPVFLGIVVVVNLMIG
                     LLTPPVGLLLYVVCGMTKLSLERVVKGVLPFLCVEIVVLFIITYIEPLSMFLPRYFGY
                     VR"
     gene            1279..1797
                     /locus_tag="VFL04_03280"
     CDS             1279..1797
                     /locus_tag="VFL04_03280"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET7838758.1"
                     /translation="MASTGPLIVITGFMGTGKTTVGRLLARRLGLDFADMDLCIEAEE
                     GAPVAAIFRERGEAYFRARERHWCERLSKRDSLVVSTGGGALVDARNLACFSLAFVVC
                     LEAEPEELLARLAAQEGRPLLEGPDPRERVARLMAERREAYARIAWHLDTSGKTADQV
                     ADEIMAVLGAQG"
     gene            complement(1850..3124)
                     /gene="aroA"
                     /locus_tag="VFL04_03285"
     CDS             complement(1850..3124)
                     /gene="aroA"
                     /locus_tag="VFL04_03285"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET7838759.1"
                     /translation="MRRSVGRSGIGGSSVAPPSKSSMQRAIACASLARGESFLSNPSG
                     SADCRAALGVAHGLGAGFEWKRGGVSIQGARPGLGLATAHRILSCGESGLCMRMFSPI
                     AALFPGETILEAEGSLRRRPLASLEGALEPLGASCRTAEGFAPLSVRGPLLGGAARVD
                     GRESSQFVTGLLIALAAAPSDSRLEVEGMVSGGYLGLTIDTMRAFGAQARSPGGGIFE
                     IAGGQAYSPARFAVEGDWSGAAFLLVAGAVAAAGDPLRVGGLGLGSSQPDRAILDALR
                     AAGAALETGPDWVEVGRSRLEAFKFDATDCPDLFPPLVALASVCEGESLIGGAQRLRH
                     KESDRAAALAETIVRLGASVRVEGDLMRIEGRPRLKACVIDSHGDHRIAMAAAVAALL
                     AEGEVVIDGAECVAKSWPRFFEDLDALRRPPA"
BASE COUNT          463 a         1134 c         1025 g          511 t
ORIGIN      
        1 tcaagggcgt gtccctcggg ctcatcgcca cccgtctctt cacgggcctc gacagcttca
       61 ccttcatggc gatccccttc ttcatcctcg ccggcgagct catgaacagc tcggggatca
      121 ccaagcggct cgtcaatttc acctcggtct tcgtgggacg gctgcgcggc gggctcggcc
      181 atgtgagcgt cgtgacctcg atgttcttcg ggggggtcac gggctcggcg atcgcggact
      241 gctcggccgt gggcacgatg atgatcccgg ccatgaagga gaagggctac gacgaggagt
      301 tcgcggccgg cctcatcgcc tcctcggcca tcatgggacc catcatcccc ccgagcatac
      361 ccttcgtcgt ctacgccctg ctttcctcga cctcggtggc ggccctcttc ctcggcggca
      421 tcgtgcccgg cgtgatgatc gccatcggct gcatggccgt gacctacgtc atcgccacga
      481 ggcgcgattt cccccggggc gagcgggtca ggagcctcgg ccagttcctc agggccctcg
      541 ccgacggctt catccccctc atgatgccct tcatcatcct ggggggcatc atcggcggca
      601 tcttcaccgc caccgaggcc tcggcggtcg cggtgctgta cggcctcgtg gtcgggctca
      661 tcgtctaccg ggacctgccg gtgagcgcca tcaggacggc cctcgcgggc acagccaaga
      721 cgaccggcgt cgttttcctc gtgatcgcct gctccaacgt cttcaactgg atgctggtcg
      781 tcgagcaggt gccccaggcc atggccagct tcgtctccgg cttcatccac gaccgctggg
      841 cgatcctcct cgcgatcaac gtcatcctcc tcatcgtcgg caccttcatg gaggggacgg
      901 cggcgatgat catcctcgtg cccatactcc tcaagatcac ggcggtctac cacatccacc
      961 ccgtcttcct cggcatcgtc gtcgtggtca acctcatgat cggcctcctc accccgccgg
     1021 tcggcctcct cctctacgtc gtctgcggca tgaccaagct gtccctcgag cgcgtcgtga
     1081 agggcgtcct gcccttcctc tgcgtggaga tcgtcgtcct cttcatcatc acctacatcg
     1141 agcccctgtc gatgttcctg ccgcggtatt tcggatacgt ccgctagtcc gggcctgggc
     1201 tccccagggg gcccgggccc gcggcccgcg atggacgaag ctcccgctac gcactagact
     1261 cgccgcaagg aacgaaggat ggcgagcaca ggtcccctca tagtcatcac cggcttcatg
     1321 ggcaccggga agaccacggt cgggaggctc ctcgcccgca ggctcgggct cgacttcgcc
     1381 gacatggacc tctgcatcga ggcggaggaa ggcgctcccg tcgctgccat cttcagggag
     1441 cggggcgagg cctatttccg cgcgcgcgaa cggcactggt gcgagcggct ctcgaagcga
     1501 gacagcctcg tcgtctcgac gggcgggggg gccctggtcg atgcgcgcaa cctcgcctgc
     1561 ttctccctcg ccttcgtcgt ctgcctcgag gccgagcccg aggagctcct ggcccggctc
     1621 gccgcccagg aaggaaggcc cctcctcgag ggtcccgatc cccgcgagcg cgtggcccgg
     1681 ctcatggccg agaggcgcga ggcctacgcg aggatcgcgt ggcaccttga caccagcggc
     1741 aagacggccg accaggtcgc cgacgagatc atggccgtcc tgggcgccca gggctgagct
     1801 gggccaagcc gtggccgccc cgccgcgggc cgcggcgatg gacgggccgc taggccggag
     1861 ggcgcctcag ggcgtcgagg tcctcgaaga accggggcca ggacttggcg acgcattctg
     1921 ccccatcaat gacgacctcg ccctcggcca ggagggcggc caccgccgcc gccatcgcaa
     1981 tccggtggtc gccgtgcgag tcgatgacgc aggctttgag cctgggcctg ccttcgatcc
     2041 gcatgaggtc gccctcgacc cttacggagg cgccgagccg gacgatggtc tcggccaggg
     2101 ccgcagccct gtcgctctcc ttatgcctga gcctctgggc cccccctatc aggctctcac
     2161 cctcgcagac ggaggcgagg gccaccaggg gcgggaaaag gtcggggcag tcggtggcgt
     2221 cgaacttgaa ggcctcgagc ctcgatctcc cgacctccac ccagtcgggc ccggtctcga
     2281 gggcggcccc ggctgctcgg agggcgtcaa ggatggcccg gtcgggctgg gaggatccaa
     2341 gaccgagccc cccgacgcgg agggggtccc cggccgccgc gacggcgcct gcgacgagga
     2401 ggaaggccgc tccgctccag tccccctcga ccgcgaatcg cgcgggactg taggcctgcc
     2461 caccggcgat ctcgaagatc ccgccaccgg ggctccgggc ctgggcgcca aaggcgcgca
     2521 tcgtgtcgat cgtgagacca aggtagccgc ccgagaccat gccctcgact tcgagtcggg
     2581 agtcggacgg ggccgcggcc agggcgatga ggagtccggt gacgaactgg gaactctccc
     2641 tcccgtcgac cctggccgcg ccgccaagca gggggccgcg cactgagagc ggggcgaaac
     2701 cctcggctgt cctgcaggag gccccgaggg gctcgagcgc gccctcgagc gacgcaagcg
     2761 gcctcctcct caaggagccc tcggcctcga ggatggtctc cccggggaag agggcggcga
     2821 tggggctgaa catcctcatg cagagaccgg attcaccgca ggagaggatc ctgtgcgcag
     2881 tggccaggcc cagcccgggt ctcgctccct gtattgagac cccgcctcgc ttccactcga
     2941 agccagcgcc aagaccatga gccacgccaa gggcagcgcg gcagtcggcc gagccgctcg
     3001 gattggacag aaacgactcg ccccgggcaa ggctggcgca ggctatggcc ctttgcatcg
     3061 agcttttcga tggcggggcg acgcttgagc ccccgatgcc cgatctgccc acgcttcgtc
     3121 tcatgacagg atg
//
LOCUS       DASSQV010000190         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_46950, whole genome shotgun sequence.
ACCESSION   DASSQV010000190 DASSQV010000000
VERSION     DASSQV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46950"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VFL04_03290"
     CDS             complement(<1..439)
                     /locus_tag="VFL04_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838760.1"
                     /translation="MAKAPAPGKDELAHWTGIEAGDFLSLTDPQSFAEGGAAGLDYRV
                     REVRRISVPEATGRGELASYHIHELEREGSGSLYLVLVAAAGQFELRLYFSPSGFASG
                     TRDRLVDLGQTWLFLPPPDPEDFLSSELEDAPYPDIPPVPEAGR"
     gene            complement(445..1227)
                     /locus_tag="VFL04_03295"
     CDS             complement(445..1227)
                     /locus_tag="VFL04_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838761.1"
                     /translation="MARNAHPRPPRAAWSRAFAVAAAFLAFASFFPAEAFARGGFRGG
                     GFRSAPSIRSMSIGRPSGSSRTGGSLFSWGSATRPAAAPSSGGPSGQGGLSSQGGAPS
                     SRFAVPGTRSSVSAQRSLYDSARSGGTLFSSKAQAAQAFRTSYGSRYGSSFATEPQTR
                     PDYIPSRTYVGGQAVNIVYNQGLGGYGYYNPMLGSWMLYDALADAAMLDILMGRHNYY
                     YGPAPVYLSHGGGFLSAALGLFLAFMLVAVLVRALRMARGRW"
     gene            complement(1232..2116)
                     /locus_tag="VFL04_03300"
     CDS             complement(1232..2116)
                     /locus_tag="VFL04_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HET7838762.1"
                     /translation="MDALILAATAALSLFPYFLLALALFLLGKYFFDWTTPRIDDNKE
                     LTVRDNPAFGVLLAGYLLGLGLALGASLLQLSPSPWQNLRDIATSGIASIVLLRLSLL
                     IGDALILRPFGINDEIVRDRNLGVGLAFGGLMTAGGLVTAGVMSGSSSGYLTMLRDIG
                     VYWLLGQAFLLAAWLLFRLVSRIDLGSELGERDNPAAGLSLAGFFIAAGIVLRAALTG
                     AGSQLGSELLVSLALAVLGLLVLGLARGLVSLVLLPRSNVADEIGAQKNTAAAVVTAV
                     GSIAVALLYAALVGSQVG"
     gene            complement(2183..>2961)
                     /locus_tag="VFL04_03305"
     CDS             complement(2183..>2961)
                     /locus_tag="VFL04_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838763.1"
                     /translation="RNTGSRLRVLTPAPNGAAARAALAGLKAHGQVKLAELESWKELP
                     AAVRDQGHGRIALFLLCVRPGEPAWHPGLEKLPQRLAEDFPELPLFLCYLPVSPEAAG
                     VPVDEGAGEKAEAGLFEEALEAGRVRPALASPAITDAVRELLRAGFGGERSLLSRLTG
                     LFTDIAQRQPIELKPGIVLLHAHVSEASEPLVFFGARPEGFRLLALERPARIIVLLCA
                     PAEQGPEEHLRTLGEIARLFKDGSLAARLGVVDEEPEPRD"
BASE COUNT          464 a          963 c         1149 g          385 t
ORIGIN      
        1 ccctcccggc ctcggggacg gggggaatgt cggggtaggg cgcgtcctcg agctcggagg
       61 agaggaagtc ctcggggtcg ggcgggggaa ggaagagcca ggtctggccc aggtcgacta
      121 gcctgtccct cgtgcccgag gcgaagcccg aggggctgaa gtacagacgc agctcaaact
      181 ggccggccgc cgcgacgagg acaaggtaga ggctgcccga gccctcgcgc tcgagctcgt
      241 ggatatggta gctcgccagc tccccgcggc ccgtggcttc ggggacggag atccgcctca
      301 cctctcgaac tcggtagtcg aggcctgccg ctcccccctc ggcgaagctc tgggggtcgg
      361 tcagggacag gaaatcgccg gcctcgatcc ccgtccagtg ggcgagctca tcctttcccg
      421 gcgcaggggc cttcgccatg cgggctacca gcgcccccgc gccatccgga gggccctgac
      481 aaggacggcg acgagcatga aggccaggaa gagccccagg gcggccgaga ggaagccccc
      541 gccgtggctg aggtagaccg gggccgggcc atagtagtaa ttgtgacggc ccatgaggat
      601 gtcgagcatc gcggcgtcgg cgagggcgtc gtagagcatc cagctgccga gcatggggtt
      661 gtagtatcca tagccgccca ggccctggtt gtacacgatg ttcacagcct gtccgcccac
      721 ataggtcctg gagggtatgt agtcggggcg ggtctgcggc tcggtggcga agctcgagcc
      781 gtagcgggag ccgtagctgg tcctgaaggc ctgggccgcc tgggccttgc tggagaagag
      841 ggttcccccc gagcgggccg agtcgtagag gctcctctgg gccgagacgc tcgagcgcgt
      901 gcccgggacg gcgaacctcg agctcggcgc cccgccctgg ctcgaaagcc cgccctggcc
      961 cgagggcccg cctgaggacg gcgcggccgc cggcctcgtc gcgctgcccc atgagaagag
     1021 gctcccgccc gtcctcgagc tcccgctcgg cctgccgatg ctcatggacc tgatggaggg
     1081 cgcgctccgg aagccgccgc ccctgaagcc cccgcgcgcg aaggcctcgg cggggaagaa
     1141 ggacgcgaag gcgaggaagg ccgcggcgac cgcgaaggcc ctggaccatg ccgcgcgggg
     1201 cggccttgga tgcgcgttgc gtgccattgg cctagcccac ctgggagccg acgagggcgg
     1261 cgtagagcag ggcgacggcg atggagccca cggccgtgac gacggcggcg gccgtgttct
     1321 tctgcgcccc tatctcgtcc gccacgttcg accgaggcag gaggacgagg gagacgaggc
     1381 ccctcgcgag gccgaggacg aggaggccga ggacggcgag ggcgaggctc acgaggagct
     1441 cgctcccgag ctggcttccg gccccggtca gcgctgccct gaggacgatg cccgccgcta
     1501 tgaagaagcc cgcgaggctg aggccggccg cggggttgtc cctctccccc agctcggagc
     1561 cgaggtcgat gcgcgagacg aggcggaaga ggagccaggc ggcgaggagg aaggcctgac
     1621 ccaggagcca gtacacgccg atgtccctga gcatcgtgag gtagcccgag ctcgagcccg
     1681 acatgacgcc ggcggtgacg aggcccccgg ccgtcatgag gccgccgaag gcgaggccga
     1741 ccccgaggtt gcggtccctc acgatctcgt cgttgatccc gaagggcctc aggatgaggg
     1801 cgtcgccgat gaggaggctc agcctgagga ggacgatcga cgcgatgccc gatgtcgcga
     1861 tatcgcggag gttctgccag ggcgaggggg agagctggag gagggatgcg ccgagggcca
     1921 agccaaggcc gaggaggtag ccggcgagga ggacgccgaa ggccgggttg tcccgcaccg
     1981 tgagctcctt gttgtcgtcg atcctcggcg tggtccagtc gaagaagtac ttgcccagga
     2041 ggaagagggc gagggcgagg aggaaatagg ggaagaggct cagggccgcg gtggcggcga
     2101 ggatgagggc gtccattggc gtctccttgg cggataaggt acatgcgggg ccgcggccgg
     2161 tcaagaaagc gcgatgtgcg cctcagtccc tgggttctgg ctcctcgtcc acgacgccga
     2221 gccgggcggc caggctcccg tccttgaaga gcctcgcgat ctcgcccagg gtccgcaggt
     2281 gctcctcggg gccctgctcg gccggggcgc agagcaggac gatgatcctg gcggggcgct
     2341 cgagggcgag gaggcggaag ccctcgggcc tggcgccgaa gaagaccagg ggctcgctcg
     2401 cctcgctcac gtgggcgtgg aggaggacga tgcccggctt gagctcgatg ggctgcctct
     2461 gggcgatgtc ggtgaagagg cccgtgagcc gcgacaggag gctcctctcg ccgccgaaac
     2521 ccgcgcggag cagctcgcgc accgcgtcgg tgatggcagg ggaggcgagg gcggggcgga
     2581 ccctcccggc ctcgagggcc tcctcgaaaa gccccgcctc ggccttctcc cccgccccct
     2641 cgtcgacggg gacgccggcg gcctcgggac tcacgggcag gtagcagagg aagaggggaa
     2701 gctcggggaa atcctcggcg agacgctggg ggagcttctc gagcccggga tgccaggccg
     2761 gttcgcccgg ccggacgcag aggaggaaga gggcgatcct gccgtggccc tggtcgcgga
     2821 ccgccgccgg cagctccttc caggactcga gctcggccag cttcacctgg ccgtgggcct
     2881 tgagcccggc gagggccgct ctcgccgccg ccccgttcgg cgccggggtc aggaccctga
     2941 ggcgggagcc cgtgttcctg a
//
LOCUS       DASSQV010000191         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2410152, whole genome shotgun sequence.
ACCESSION   DASSQV010000191 DASSQV010000000
VERSION     DASSQV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410152"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1158)
                     /gene="epsC"
                     /locus_tag="VFL04_03310"
     CDS             complement(247..1158)
                     /gene="epsC"
                     /locus_tag="VFL04_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HET7838764.1"
                     /translation="MDVLDRAIEALLESYENVGGVNHSGGPNLPSRAAVVGALSDIEA
                     LVFPGFSAEEGIDDLSLGFVTGELVHRAAKILVRECTRSLEYKYRTAGQEGCPAACDL
                     EARDLVVRFFEALPGIRAVLMEDVAAAFAGDPAAKTIAEVILAYPGLEALVVHRVAHF
                     FWLEGLPLIPRMMSEAVHGTTGIDIHPGASIGRRFFIDHGTGVVIGETAVIGDDVKLY
                     QGVTLGALSIRKEEGGMKRHPTIEDEVTIYSGATILGGATVVGRGSTIGGNVWLTHSV
                     PAWSKVYLDEGDSARVESRYMDKGACI"
     gene            complement(1161..>2128)
                     /locus_tag="VFL04_03315"
     CDS             complement(1161..>2128)
                     /locus_tag="VFL04_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7838765.1"
                     /translation="LLAMIVALNVFKQGSRGPVVALMPDLIPGEYRSEANGVINMSSA
                     VAAIIGTLFLARLMDLDLVLPVLGQVRGRLPFLLAALLVVITVALLFGLVKERRTEAG
                     AGADEERVGVWSSIALVLGSKDRSAPLILASIFLWFFGYQGVLPFLSTYMREVIGVSL
                     GQTAMAPGAVAVAMTLCAVPSGYIAHRVGRRRTIRFALMALALVLLALFAHGSLSSAL
                     GLGHGARLASFLGLMFLFGAFWIAIVANSFPMLWQMASHANIGVYTAVYYAAKESSSI
                     LSPPVTGAIADLLGMRWMFAVAALCMAAAFFVMRGATSGEAPREV"
BASE COUNT          336 a          698 c          769 g          325 t
ORIGIN      
        1 ggccgccggc gctatgtatc aatccgcctc ggaggccttg ctgtccttct tggccttctt
       61 ctcggcccgc ttctccttga gggtcttgac cggcttcttt ttctcctcgc ccttctgctt
      121 gtcctgcgat ttcatatgca ctcctcccgg cccttctcga gccggccccg gccatcgggg
      181 ccccgcgctc ttcgggccga taccaccacc attctacaac ctcggccgtt tttttcaagg
      241 cggggcctag atgcaggccc ccttgtccat gtagcgggac tcgaccctgg ccgaatcgcc
      301 ctcgtcgagg tagaccttgg accaggccgg gaccgaatgg gtgagccaga cgttgccgcc
      361 tatcgtcgag ccccggccga ccaccgtggc cccgccgagg atcgtcgcgc ccgagtagat
      421 cgtgacctcg tcctcgatgg tggggtggcg cttcatcccg ccctcctcct tgcggatcga
      481 gagggccccg agggtgacgc cctggtagag cttcacgtcg tcgccgatga cggccgtctc
      541 gccgatcacc acgccggtgc cgtggtcgat gaagaagcgc ctgccgatgc tggcccccgg
      601 atggatgtcg atgcccgtcg tgccgtgcac tgcctcgctc atcatccgag ggatgagggg
      661 gaggccctcg agccagaaga agtgggcgac gcggtgcacg acgagggcct cgaggccggg
      721 gtaggccagg atcacctcgg ctatggtctt ggcggccggg tcgccggcga aggccgcggc
      781 gacgtcctcc atcaggaccg ccctgatccc gggcagggcc tcgaagaagc ggacgacgag
      841 gtcgcgggcc tcgaggtcgc aggccgcggg gcagccctcc tggccggcgg tccggtactt
      901 gtactcgagg ctcctcgtgc actcccgcac gaggatcttg gcggcccggt gcacgagctc
      961 gccggtgacg aagcccaggc tcaggtcgtc gatcccctcc tcggcgctga agccggggaa
     1021 gacgagggcc tcgatgtcgc tcagggcccc gacgacggcg gcgcggcttg gcaggttggg
     1081 cccgcccgag tggttcaccc cgccgacgtt ctcatatgac tcgaggaggg cctcgatggc
     1141 cctgtcgagg acatccatgg tcagacctcc cgcggcgcct ctcccgaggt ggcgccgcgc
     1201 atgacgaaga atgccgcggc catgcagagg gccgcgacgg cgaacatcca gcgcatcccg
     1261 agcaggtcgg cgatggcccc cgtcaccggg ggagagagta tcgagctcga ctccttggcg
     1321 gcgtagtaga ccgccgtgta gacgccgatg ttcgcgtggc tcgccatctg ccagagcatg
     1381 gggaaggagt tggcgactat cgcgatccag aaggcgccga agaggaacat gaggcccagg
     1441 aagctcgcga gcctcgcgcc gtggcccagg cccagggccg aggagaggct gccgtgggcg
     1501 aagagggcga ggaggacgag ggcgagggcc atgagggcga agcggatggt ccgcctcctg
     1561 ccgacgcgat gggcgatgta gcccgagggg acggcgcaga gggtcatggc cacggccacg
     1621 gccccggggg ccatggccgt ctggccgagg gagaccccga tcacctcgcg catgtaggtg
     1681 ctgaggaagg gcaggacgcc ctggtagccg aagaaccaga ggaagatgga ggccaggatg
     1741 agcggggcgc tgcggtcctt cgagccgagg acgagggcga tggagctcca gaccccgacc
     1801 cgctcctcgt ccgccccggc gcccgcttcg gtcctcctct ccttcacgag gccgaagagc
     1861 agggccacag tgatgacgac gaggagggcg gcgaggagga aggggagcct gcccctcacc
     1921 tggcccagga cggggaggac gaggtcgagg tccatgagcc gggcgaggaa gagggtgccg
     1981 atgatcgccg cgacggcgct cgacatgttg atgaccccgt tggcctcgga gcggtactcg
     2041 ccggggatca ggtcgggcat cagggcgacg acggggccgc gggagccctg cttgaagacg
     2101 ttgagcgcga cgatcatggc gaggaggg
//
LOCUS       DASSQV010000192         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1788652, whole genome shotgun sequence.
ACCESSION   DASSQV010000192 DASSQV010000000
VERSION     DASSQV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788652"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VFL04_03320"
     CDS             complement(<1..983)
                     /locus_tag="VFL04_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7838766.1"
                     /translation="MNGQGILIVEDESIVALDMRMRLEAMGYRVVDIVDTGALALERA
                     ASAGPDLILLDIKLKGPMDGIETARALRGIASIPVIFVTAFADEKTLQRAKLASAYGY
                     IIKPYHERELRVAIELALYKHEYELSVLRARDLAEEANRLKGEFLANISHELKTPLNS
                     VIGFVQLGLDRADDDEQRENLGMALGGARSLLDLIDSILALTKMEAGRLTPASLPFSL
                     GTLVGECVDILAMNASRKGLDASFRWEPGLPDRVVGDEALVRRVMLNLIDNATKFTEA
                     GRVRLELSHAEGRGERAQTLPLLISVTDTGIGMPPEKVESAFERFTQLDGS"
     gene            complement(980..1447)
                     /locus_tag="VFL04_03325"
     CDS             complement(980..1447)
                     /locus_tag="VFL04_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7838767.1"
                     /translation="MNGERSGRYLAFDLGGRQYAIEVERVEVVLESVPITRVPRARPH
                     LRGVINYRGAVIPVADLRMRFGEGETGTGEGTSIIVMQIHYGSEDVTIGMLADSVREV
                     IDIDAERIERAPRIGSRVEDSLVAGVGKREGDFVVLLDIDEAFKAEAATAEAR"
     gene            complement(1461..1829)
                     /locus_tag="VFL04_03330"
     CDS             complement(1461..1829)
                     /locus_tag="VFL04_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7838768.1"
                     /translation="MPKKILIVDDSAAIRQSISFILQQEGYETVEATDGLEALGLLEG
                     GPALDLIITDVNMPNLDGIGFIRKARELPSFKFTPILVLTTESQGSKMNEGKEAGATG
                     WIVKPFNADKLLGIVKKVAE"
     gene            complement(1893..>2095)
                     /locus_tag="VFL04_03335"
     CDS             complement(1893..>2095)
                     /locus_tag="VFL04_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET7838769.1"
                     /translation="GVIMTGMGDDGSRGMREMHDAGAYTIAQDEASCVVFGMPNEAIK
                     HGGVDTVLALGGIADEVQRRAR"
BASE COUNT          311 a          705 c          684 g          395 t
ORIGIN      
        1 ctcgagccgt ccagctgggt gaagcgctcg aatgccgact cgaccttctc cgggggcatg
       61 ccgatcccgg tgtcggtgac ggagatgagg aggggaaggg tctgggccct ctcgcccctc
      121 ccctcggcgt ggctgagctc gagcctgact cggccagcct cggtgaactt cgtggcgttg
      181 tcgatgaggt tgagcatgac ccgcctgacc agggcctcgt ccccgacgac ccggtcgggc
      241 aggcccggct cccagcggaa actggcgtcg aggccctttc tcgaggcgtt catcgcgagg
      301 atgtcgacgc actcgccgac gagggtccca agcgagaaag gcagggaggc aggggtaagg
      361 cgcccggcct ccatcttcgt taacgcgagt atcgaatcga tgaggtcgag gagggagcgg
      421 gcgcctccga gggccatgcc gaggttctcg cgctgctcat cgtcgtcggc ccgatcgagg
      481 cccagctgga cgaagccgat gacactgttg aggggggtct tgagctcatg gctgatgttc
      541 gcgaggaact cgcccttgag gcggttggcc tcctcggcca gatccctggc cctcagcacc
      601 gacagctcgt attcgtgctt gtagagggcc agctctatgg ccacgcgcag ctcgcgctcg
      661 tggtagggct tgatgatgta gccgtaggcg ctcgccagct tcgcccgctg gagggtcttc
      721 tcgtcggcga aggcggtcac gaagatgacg gggatggagg cgatgccgcg cagggcccgc
      781 gcggtctcga tcccgtccat ggggcccttg agcttgatgt caaggaggat caggtcgggc
      841 cccgcgctcg ctgcccgctc caaggccagg gctccggtgt cgactatgtc gacgacgcgg
      901 tagcccatcg cttcgaggcg catgcgcatg tcgagggcga cgatgctctc gtcctcgaca
      961 atcaggatgc cctggccgtt catctggcct ctgccgtcgc cgcttccgcc ttgaaggcct
     1021 cgtcgatgtc cagaaggacg acgaaatcgc cctcgcgttt gcccactccg gccacgaggg
     1081 aatcctcgac cctgctcccg atccggggcg cgcgctcgat gcgctcggcg tcgatgtcga
     1141 tcacctcgcg caccgagtcg gccagcattc cgatcgtcac gtcctcgctg ccgtagtgga
     1201 tctgcatcac gatgatgctc gtcccctcgc ccgtccccgt ctcgccctcc ccgaaccgca
     1261 tgcggaggtc ggccacgggg atcacggctc cgcggtagtt gatcacgccc ctgaggtggg
     1321 gcctcgccct cggcaccctc gtgatgggga cggactcgag gacgacctcg acgcgctcca
     1381 cctcgatcgc gtactgccgg cccccgaggt caaaggcgag atagcgcccg ctccgctctc
     1441 cgttcatggc gcgagcccgc tcactcggcg actttcttta cgatgccgag gagcttgtcg
     1501 gcgttgaagg gcttcacgat ccagccggtc gctccggcct ccttgccctc gttcatcttc
     1561 gagccctggg actcagtggt caggaccagg atgggcgtga acttgaagga cggtagctcc
     1621 ctggccttgc ggatgaagcc gatgccgtcg aggttgggca tgttcacgtc ggtgatgatg
     1681 aggtcgaggg ccgggccgcc ctccagcagg ccgagggcct ccagaccatc ggtcgcctcc
     1741 acggtctcgt atccctcctg ctggaggatg aaggagatgc tctggcggat cgcggcagag
     1801 tcgtcgacga taaggatctt cttgggcatc atggctcctt ttccgtacag gtctatttca
     1861 atgccggctc cccgagccgg ataccctcgc cgtcatcttg cccgacgctg gacctcgtcg
     1921 gcaataccgc ccagggcgag gacggtgtcg actcccccgt gcttgatcgc ctcgttgggc
     1981 atgccgaaga ccacgcagct cgcctcgtcc tgggctatcg tgtaggcgcc ggcgtcgtgc
     2041 atctcccgca tcccccgcga gccgtcgtcg cccatcccgg tcatgatcac gccga
//
LOCUS       DASSQV010000193         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_295786, whole genome shotgun sequence.
ACCESSION   DASSQV010000193 DASSQV010000000
VERSION     DASSQV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295786"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="VFL04_03340"
     CDS             complement(<1..1517)
                     /locus_tag="VFL04_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HET7838770.1"
                     /translation="MDIVERTIGDRGLSYRQKVSALARLGEERVSPLRIPPRTEELRA
                     AGVVCDLNEGPAPYRPRYILPDYDAFLRRGSAFLRIEAPRDFLDAVFALLAIYRQVPS
                     ITGYPVWLGDLDSLLEPFALPDAGDALAPEQDRLVRMFMEQIDRDLPDSFCHADLGPR
                     ATRVGRAILRHQRSAARAVPNLSLRYDPELTDDGFAEEAAACALDSAKPSFANHRRFE
                     ADFAALGLGRYAVASCYNGLPVGGGSCTLVRLNLAALARSARIEGMGVEDFLSGPLAE
                     AVAATLGYIQERVRFVMEDSGFFESSFLAAEGLIERSRFTAMFGLVGLAECVNALLAR
                     EGARFGRDKAADELGLRVLGRIDDLVAAHRDPRLEAAGGRYLLHAQVGIDSDTGVSPG
                     CRIPIGEEPSLPEHLLRSAPFHAPFVAGTGDVFAFEPTARANPGQIVDAVKGFFALGG
                     RYFSCYAADSDVFRVTGYLVKRSEVERLRRGEAVPNASTVLGMNAIDNLKVLDRAL"
     gene            complement(1522..2199)
                     /locus_tag="VFL04_03345"
     CDS             complement(1522..2199)
                     /locus_tag="VFL04_03345"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HET7838771.1"
                     /translation="MNRTTFVLAASLAALALGCAPRSSGTAASPKAATAATAPKANLV
                     YLEGSVKIDGAAAELGQTLGPGFSVETGANSVCDVVFGGKNIFRVGQNAFVKVDFTKQ
                     APEFELQRGGLTSVLKKLDLIAGQDSYRVRTNTAVAGVRGTSFCIWADEKSTYVCACN
                     GSVHTIDAKGSNELSLKAAHHTARIYSLAGAGETVAEAGILHHDDATVQAIATKIGYT
                     VDWTVAD"
     gene            2289..>2603
                     /locus_tag="VFL04_03350"
     CDS             2289..>2603
                     /locus_tag="VFL04_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838772.1"
                     /translation="MNDSIFMPSPPQNERSLGYALGSPERARLEAELERLLSAGTEIP
                     CLIGGKELRTGRTRPIIRPDERQTELGLYHLAGAPEARAAIEAALEAKRGWEAMPWEE
                     RAA"
BASE COUNT          411 a          841 c          977 g          374 t
ORIGIN      
        1 agggcccggt ccagcacctt gaggttgtcg atcgcgttca tgccgaggac ggtcgaggcg
       61 ttgggcacgg cctcgccgcg gcggagcctc tcgacctcgg agcgcttgac gaggtagccg
      121 gtgacgcgga agacatccga atccgccgcg tagcaggaga agtagcggcc accgagggcg
      181 aagaagccct tgacggcgtc gacgatctgg cccgggttgg ccctggccgt gggctcgaag
      241 gcgaagacat cccccgtgcc ggccacgaag ggggcgtgga agggggccga gcgcagcaga
      301 tgctcgggca ggctcggctc ctcgccgatg gggatgcggc aacccgggga cacgcccgta
      361 tcggagtcga tgcccacctg ggcgtgaagg agatagcggc cgccggcggc ctcaagcctc
      421 gggtcccgat gggcggcgac gagatcgtcg atccggccga ggacgcgcag gcccagctcg
      481 tcggcggcct tgtcgcggcc gaacctggcg ccctcgcggg cgaggagggc gttgacgcat
      541 tcggcgaggc ccacgaggcc gaacatcgcg gtgaagcgcg agcgctcgat gaggccctcg
      601 gcggctagga aggaggactc gaagaagccc gagtcctcca tgacgaagcg cacgcgctcc
      661 tggatgtagc cgagggtggc cgccacggcc tcggccaggg ggccggagag gaagtcctcg
      721 acccccatgc cctcgatccg cgcggagcgg gcgagggcgg cgaggttgag gcgcacgagg
      781 gtgcagcttc ccccgcccac ggggagcccg ttgtagcagc ttgcgaccgc gtagcggccc
      841 aggccgaggg cggcgaagtc ggcctcgaag cggcggtggt tggcgaagct cggtttcgcc
      901 gagtcgaggg cgcaggcggc ggcctcctcg gcgaagccat cgtcagtcag ctcggggtca
      961 tagcggaggc tcaggttcgg cacggctcgg gcggccgaac gctggtggcg aaggatggcc
     1021 ctgccaaccc gcgtggcgcg gggaccgagg tcggcgtggc agaaggagtc gggcaggtcg
     1081 cggtcgatct gctccatgaa cattcgcacg agcctgtcct gctcgggcgc gagcgcgtcc
     1141 ccagcgtccg gcagggcgaa gggctcgagc agggagtcca ggtccccgag ccagaccggg
     1201 tagcccgtga tcgaggggac ctgtctgtag atcgcgagga gggcgaagac ggcgtcgagg
     1261 aagtcgcgtg gggcctcgat ccggaggaag gccgagccgc ggcggagaaa ggcatcgtag
     1321 tcgggcagga tgtagcgcgg ccggtagggc gccgggccct cgttgaggtc gcagacgacc
     1381 ccggcggccc tgagctcctc ggtgcgcggt gggatgcgga ggggggagac cctctcctct
     1441 ccaagccggg ccagggccga gaccttctgc ctgtagctga ggcccctgtc cccgatcgtg
     1501 cgctcgacga tgtccatcgc cctagtccgc cactgtccag tcgaccgtgt agccgatctt
     1561 cgtggctatc gcctgcaccg tggcgtcgtc gtggtgcagg atgcccgcct cggccacggt
     1621 ctccccggcg ccggcgagcg aatagatcct cgccgtgtgg tgggcggcct tgaggctgag
     1681 ctcgttgctg cccttggcgt ctatggtgtg caccgagccg ttgcaggcgc agacgtaggt
     1741 cgatttctcg tcggcccaga tgcagaagga ggtgccacgc acaccggcga cggccgtgtt
     1801 cgtcctgacg cggtagctgt cctggccggc gatcaggtcg agcttcttga ggaccgaggt
     1861 gagcccgccc ctttgcagct cgaactccgg agcctgcttg gtgaagtcca ccttgacgaa
     1921 ggcgttctgc ccgaccctga agatgttctt cccgccgaag acgacgtcgc agaccgagtt
     1981 cgcccccgtc tccacgctga agccggggcc gagggtctgg ccgagctcgg ccgcggctcc
     2041 gtctatcttc accgagccct cgaggtagac gaggttggcc ttcggagccg tggcggcggt
     2101 cgcggccttg ggagacgcgg cggtcccgga gctccggggc gcgcagccga gggcgagggc
     2161 ggcgagggag gcggcgagga cgaaggtggt tctgttcatg cgaagaggat cgcactatgc
     2221 cccgagacaa tcaaggccca tgacggggac aggcagctgt atcttcatgg tatgaggttc
     2281 tggacgccat gaacgactcc atcttcatgc caagccctcc acagaacgaa agaagcctcg
     2341 gctacgccct cggttcaccc gagcgcgcca ggctcgaggc cgagctggag aggctccttt
     2401 ccgccgggac cgaaatcccc tgtctgatcg gcggcaagga gctgcgcacg ggccgcaccc
     2461 ggcccatcat ccgtcccgac gagcgccaga ccgagcttgg cctctaccac ctcgcgggcg
     2521 cgccagaggc ccgcgccgcc atcgaagccg ccctcgaggc caagcgcggc tgggaggcca
     2581 tgccctggga ggagagggcg gcc
//
LOCUS       DASSQV010000194         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_669730, whole genome shotgun sequence.
ACCESSION   DASSQV010000194 DASSQV010000000
VERSION     DASSQV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669730"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..783
                     /locus_tag="VFL04_03355"
     CDS             166..783
                     /locus_tag="VFL04_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET7838773.1"
                     /translation="MTVSILPRRFQEEEEESKKDLRKPEDALAERFLKTRTILLTGEI
                     DKDQGERIIRSLLMLESLSDEPVNLLIDSPGGDVDAGFAIFDMIRFVKCPVRILGMGL
                     VASAAALVLLAVPKERRFALPNSHYLIHQPLSGLRGVATEIEIHARELEKTRERINRI
                     IAEETGQTMERVQKDTDRDYWMSADEAQAYGLISRIGVRRDELGL"
     gene            780..1763
                     /locus_tag="VFL04_03360"
     CDS             780..1763
                     /locus_tag="VFL04_03360"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET7838774.1"
                     /translation="MILKEDIGARLGTSFDSLPEAAACGLPVYRGKVRDVVRGKDLLL
                     LVASDRISAFDRVLSTVPFKGEILARVSAWWFSRCADIVANHVVQGGTPEELGGTGRA
                     CLVRRAEMIPVEVVVRGYLAGSAWRDYHEGRPVSGIELPEGLSMNEKFPVPLITPSTK
                     EEGAHDRPLSREEIVAEGILEEDRWAEIERVSVALFRRGQELAARAGLILVDTKYEFG
                     ILDGGLVLADEIHSPDSSRYWWEDSYETLFLAKESQRELDKEPFRRWLLAQGFRGEGP
                     APAIPDEVRVETAWRYIQAYEAITGEEFRPLSSDPQSESAELARIVSERLR"
     gene            1809..>2799
                     /locus_tag="VFL04_03365"
     CDS             1809..>2799
                     /locus_tag="VFL04_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7838775.1"
                     /translation="MPSFLWPAACFVLASASLLACACTSCSGLGPRSGRSISLVSYNA
                     MNLFDATDQGGEYPEFSVRGGAWDEAKYRLRLARLAEAVAPKDLVGSGPDILCLVEIE
                     NPSVIEALRSGPLKRAGYTSALMSPAEGSPINSAMLSRLPLLSLRAHATAAGPEGKAR
                     AGRRILEAEFDAGACPLVVVLCHWKSKLEGAGTTEAERREAAALVRGIVARRLAEGRP
                     AIIVCGDFNESPDEYLRVGRRYPTALMLLGEAPPGSGPPRLLVTTRRSEASSSGMEPV
                     LYSPWGESQGFSYAYSGEKARIDGFLLGSGLLAGGPPPGGPSLRYQSFSVIDPP"
BASE COUNT          435 a          993 c          915 g          456 t
ORIGIN      
        1 ccacgatgta gacgtttggc ctgcccatgt ttcaccccta tgagctttga tatatcaaca
       61 atactatgtt tggcgtctgg cgaccaagcc atcccaccgg gccgggtctg gcccctcggg
      121 cgggggaagt gtatattgta aggaaaccca agccaaggag ccgcgatgac cgtatccatc
      181 cttccccgtc gctttcagga agaggaggag gagtccaaga aggacctcag gaagcccgag
      241 gacgccctcg ctgagcgttt cctcaagacg cgcaccatcc tgctcacggg ggagatagac
      301 aaggaccagg gcgaaaggat catccggtcg cttctcatgc tcgagagcct ctccgacgag
      361 cccgtcaacc tcctcatcga ctcgcccggc ggcgacgtcg atgccggttt cgcgatcttc
      421 gacatgatcc gtttcgtgaa gtgccccgtg cggatcctcg gcatgggcct ggtcgccagc
      481 gccgccgccc tcgtccttct ggcagtgccc aaggagaggc gcttcgccct gccgaactcg
      541 cattacctca tccaccagcc cctctcgggc ctccgcggcg tggccaccga gatcgagatc
      601 cacgcgcgcg agctggagaa gacccgcgag cgcataaaca ggatcatcgc cgaggagacg
      661 ggccagacca tggagcgggt ccagaaggac accgaccgcg actactggat gagcgccgac
      721 gaggcccagg cctatggcct tatctcgcgc ataggggtgc ggcgcgacga gctcggccta
      781 tgatcctcaa ggaggacatc ggggcccgcc tcggtacttc cttcgattcc cttcccgagg
      841 ccgccgcctg cggccttccc gtctaccgcg ggaaggtccg cgacgtcgtc aggggcaagg
      901 acctcctcct tcttgtcgcg agcgacagga tctcggcctt tgaccgcgtg ctctcgaccg
      961 tgcccttcaa gggcgagatt ctcgcccggg tctcggcctg gtggttcagc cgctgcgccg
     1021 acatcgtggc gaaccatgtg gtgcagggcg ggacgcccga ggagcttggc ggcacggggc
     1081 gggcctgcct cgtgcgcagg gcggagatga tccccgtcga ggtggtcgtg cggggctatc
     1141 tcgccggctc ggcctggcgg gactaccatg agggccggcc cgtctcgggc atcgagctgc
     1201 ccgaaggcct ctcgatgaac gagaagttcc ccgtgcccct gatcaccccc tccaccaagg
     1261 aggagggggc ccacgacagg cccctgtccc gggaggagat cgtcgccgag ggcatcctcg
     1321 aggaggacag gtgggcggag atcgagcgcg tttcggtcgc ccttttccgc cggggccagg
     1381 agctcgcggc gcgggccggc ctcatcctgg tcgacaccaa gtacgagttc ggcatcctgg
     1441 acggaggcct cgtcctggcc gacgagatcc acagccccga ttcgagccgc tattggtggg
     1501 aggactccta cgagaccctc ttcctggcga aggagtcgca gcgggagctc gacaaggagc
     1561 ccttccggcg ctggctcctc gcccagggct tccggggcga gggacctgcc ccggcgatcc
     1621 ccgacgaggt cagggtggag acggcctggc gctacatcca ggcctacgag gccatcacgg
     1681 gcgaggagtt caggcccttg agctcggatc ctcaatcgga aagcgcggaa ctggcgcgta
     1741 tcgtctccga gcggctgcgc taggctggcc caatgggcgg gcctcggctg ccgtcctgga
     1801 ggccgaagat gcccagcttc ctctggcccg cggcctgttt cgtccttgcc tcggcatccc
     1861 tcctcgcctg cgcctgcacc tcatgctcgg gccttgggcc gcgttccggg cgcagcatat
     1921 ccctcgtctc gtacaacgcg atgaacctct tcgacgccac cgaccaggga ggggagtatc
     1981 ccgagttctc ggtccgcggc ggggcctggg acgaggccaa gtaccggctc cgcctggcaa
     2041 ggctggcaga ggccgtggcc cccaaggacc tggtcggctc gggccccgac atcctctgtc
     2101 tcgtcgagat cgagaacccc tccgtcatcg aggccctccg ctccggtccg ctcaagaggg
     2161 cgggctacac ctcggccctc atgtccccgg ccgagggctc gcccatcaac agcgccatgc
     2221 tgagccggct tccgctcctc agcctcaggg cccatgccac ggctgccggc cccgagggaa
     2281 aggcccgggc gggacggcgg atcctcgagg ccgagttcga cgccggagcc tgccccctcg
     2341 tcgtcgtgct ctgccactgg aagtccaagc tcgagggggc ggggaccacc gaggccgaga
     2401 ggagggaggc cgccgccctc gtcaggggca tcgtcgcgcg gaggctcgcc gaaggccggc
     2461 ccgccatcat cgtctgcggc gacttcaatg agagccccga cgagtacctg cgcgtggggc
     2521 ggcgctaccc gacggccctc atgctcctcg gcgaggcccc ccctggctca ggcccgcccc
     2581 ggctccttgt cacgacgcgt cgctcggagg cctcatcctc gggaatggag cccgtcctgt
     2641 acagcccctg gggcgagtcc cagggcttca gctacgccta ctccggcgag aaggcccgga
     2701 tcgacggctt cctcctcgga tcgggcctgc ttgccggcgg tcccccgccg ggcggcccct
     2761 ccctgagata ccagtcattc tccgtcatcg acccgccct
//
LOCUS       DASSQV010000195         5183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1663455, whole genome shotgun sequence.
ACCESSION   DASSQV010000195 DASSQV010000000
VERSION     DASSQV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663455"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFL04_03370"
     CDS             complement(<1..634)
                     /locus_tag="VFL04_03370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7838776.1"
                     /translation="MALIAPRYRGIYLALFCLFALFGISMTVIGASLPRIFAEFGWSY
                     STAGAVIAAGSVAYFLSSMAAGAALRRFGPRRAVLAGLLACTVGSAFFAASPSPVANL
                     LLNALIGAGQGFIEIVVNWSVLRMDEKATGRAMNLMHGAFAIGAVVGPLSVGILIAAD
                     MAWALLYRAIAIVFVLLAIAVAVLPFSRLGREEPEHGHRNKVAALARHPVY"
     gene            complement(640..2628)
                     /gene="uvrB"
                     /locus_tag="VFL04_03375"
     CDS             complement(640..2628)
                     /gene="uvrB"
                     /locus_tag="VFL04_03375"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET7838777.1"
                     /translation="MKPFKVVAPYGPAGDQGQAIDTLVSGVREGLRWQTLKGVTGSGK
                     TFTMAKVIEGLQMPALIVSHNKTLSAQLYREFKTFFPENAVEYFVSYYDYYQPEAYVP
                     SKDLYIEKDSDINAEIDRMRLSATAALMERRDVIIVATVSCIYGLGSPDLYKEMRLYI
                     DRGSTIDLDEVKRRLVSLQYERNDAVLERGRFRVRGDVLEIYPAYLQEAYRIELDYDT
                     VTRIRKFDTVSGAMGDELDEAVVYPAKHFVMPEDQVRNAVARIRGELDERYESLMAMN
                     KLVEAQRLKSRTEYDLEMLEEMGYCSGIENYSAPLAGRSPGERPGVLIDYFPKEFLTF
                     VDESHVTLPQVGAMYAGDRSRKTTLVDYGFRLPSAKDNRPLKYEEFLSVIDRVICVSA
                     TPQAEELEKSGRVAEQVIRPTGLVDPEIEVRPSEGQMEDIYAEVRKRIAADERCLILT
                     LTKRMAEELTEYLTGLGLKVRYIHSEVETIERVEILKQLRMGDYDVLVGINLLREGID
                     LPEVSFIAILDADKIGFLRSATSLIQIIGRAARNAAGTVVMYADRMSEAMKAAIEETD
                     RRRAKQVAYNLENGITPISIRKAIEDILERHREERQDDAAGEIELLKRSHNLLVPEQK
                     KALLKALEAQMLEHAKNLEFEQAAVLRDEIERLKKGED"
     gene            complement(2666..3907)
                     /locus_tag="VFL04_03380"
     CDS             complement(2666..3907)
                     /locus_tag="VFL04_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7838778.1"
                     /translation="MRLYSRGQVLLVALGGVVLALLLAFGLGLLQPWGGATGSGIVQS
                     SSPAGQAGLSSSAQADPRLRQTEVAAEYTQDERENIDVYERLNEGVVNITTEVVAMNW
                     FLEAVPQEGGTGSGSIIDSRGYVLTNNHVVKGAYKLYVNLADGARYEAKVVGVDPEND
                     MAVIKFDAPRNARLTVIPYGDSSGLKVGQKVLAIGNPFGFDRTLTRGIVSSLGRPIKE
                     SDTNIIRNMIQTDASINPGNSGGPLLNSRGEMIGVNTMIYSPSGASVGIGFAVPVNTA
                     KRVVPELIRDGMVRRGWVDIEPIQLFPDLIDYMKQNGYPVPVSAGLLVSQATRGGNAD
                     RAGIRGGDTAVRYGRSTFNVGGDIIVSVNGMKTATIAEFYSALETTKPGQKVTVEYWH
                     GSKKMTVDLALSNRAKTGVDQ"
     gene            4052..5158
                     /locus_tag="VFL04_03385"
     CDS             4052..5158
                     /locus_tag="VFL04_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HET7838779.1"
                     /translation="MAGQITHIIAGERALREAAPEEGEELLREQGPAFRLGCQGPDIF
                     YHNQRTKPSGLHYGALAHRRGYGRLVAGALASLPPEPSPARRQAEAYILGLATHAALD
                     RATHPFIVYFSGWHDPAIPGSERYRSCHPFLERLLDVGYLAASLGLPPAAFDIAAKLG
                     LETGSTPESLGLPKDRAGEALIVALWSSGLRSAFPRSTGSDFLLEKRVENALLDGRHF
                     YGITNPAVTALNPERKDWFGYLDDRAGPRSVCLVYPDSLPEGLDVLNLGKAAWEEPSG
                     LGTERDESYLELVEKGIHTAAVALGALLPALATEGPPGFELAAAIGDGGLSIVDAEGR
                     SVGPRVCRPLPLMEIMELEYGRRLEWAKRQLGKA"
BASE COUNT          800 a         1729 c         1755 g          899 t
ORIGIN      
        1 agtagaccgg atgcctcgcc agggccgcga ccttgttccg gtggccgtgc tcgggctcct
       61 ctcggccgag gcgggagaag ggcagtaccg cgaccgcgat ggccagaagg acgaacacga
      121 tggcgatcgc ccggtacagg agggcccagg ccatgtcggc cgcgatgagg atgccgacgg
      181 agaggggccc gaccacggcg ccaatggcga aggcgccgtg catgaggttc atggccctgc
      241 ccgtggcctt ctcgtccatc ctgagcaccg accagttcac gacgatctcg atgaagccct
      301 gccctgcgcc gatgagggcg ttcaagagga ggttggcgac cggggagggg ctcgcggcga
      361 aaaaggccga acccacggtg caggccagaa ggccggcgag gactgcccgt ctcggcccga
      421 agcgcctgag ggcggctccc gccgccatgc tcgagaggaa gtaggccacc gagcccgccg
      481 cgatcacggc ccctgccgtg gagtagctcc agccgaactc ggcgaagatc ctcggcagcg
      541 aggcgcctat caccgtcatc gagatgccga agagggcgaa gaggcagaag agggccagat
      601 agatgccgcg gtatcgcggc gcgatgagcg ccatcgtctt tagtcctcgc ctttcttgag
      661 ccgctcgatc tcgtccctga gcactgccgc ctgctcgaac tcgaggttct tcgcgtgctc
      721 gagcatctgg gcctcgaggg ccttgagcag ggccttcttc tgctcgggca ccaggaggtt
      781 gtggctccgc ttgagcagct cgatctcgcc ggcggcgtcg tcctgcctct cctcgcggtg
      841 gcgctcgagg atgtcctcga tggccttgcg tatcgagatg ggggtgatgc cgttctcgag
      901 gttgtaggcg acctgcttgg cccggcgccg gtcggtctcc tcgatcgccg ccttcatggc
      961 ctcggacatc cggtcggcgt acatgaccac ggtgccggcc gcgttgcgcg ccgccctccc
     1021 gatgatctgg atgaggctcg tggccgacct gaggaagcct atcttgtcgg cgtcgaggat
     1081 cgcgatgaag gagacctccg gcaagtcgat gccctcgcgg agcaggttga tcccgacaag
     1141 gacgtcgtag tcgcccatgc ggagctgttt caggatctcc acgcgctcga tggtctccac
     1201 ctcggagtgg atgtagcgga ccttgaggcc gaggccggtg aggtactcgg tgagctcctc
     1261 ggccatgcgc ttggtgaggg tgaggatgag gcagcgctcg tccgcggcta tgcgcttccg
     1321 gacctcggcg tagatgtcct ccatctggcc ctcgctcggc ctgacctcga tctcggggtc
     1381 gacgaggccg gtgggcctga tcacctgctc ggccacccgg cccgatttct cgagctcctc
     1441 ggcctggggg gtcgccgaga cgcagatgac gcggtcgatg acggagagga actcctcgta
     1501 cttgaggggg cggttgtcct tcgcgctcgg gaggcggaag ccgtagtcca ccaaggtggt
     1561 cttgcggctg cggtccccgg cgtacatggc ccccacctgg ggcagggtga cgtgggactc
     1621 gtcaacgaag gtgaggaact ccttggggaa atagtcgatg aggaccccgg gcctctcccc
     1681 gggggagcgt cccgcgaggg gggccgagta gttctcgatc cccgagcagt agcccatctc
     1741 ctcgagcatc tcgaggtcgt actcggtgcg gctcttgagc ctctgggcct cgacgagctt
     1801 gttcatggcc atgaggctct cgtagcgctc gtcgagctcc ccccgtatgc gggccaccgc
     1861 gtttcgcacc tggtcctcgg gcatgacgaa gtgcttggcc gggtacacca cggcctcgtc
     1921 gagctcgtcg cccatcgcgc ccgagaccgt gtcgaacttg cggatgcgcg tcaccgtgtc
     1981 gtagtccagc tcgatcctgt aggcctcctg gaggtaggcc ggatagatct cgaggacgtc
     2041 gcccctgacc ctgaagcgcc cccgctcgag gacggcgtcg ttgcgctcgt actggaggct
     2101 gacgagcctg cgcttgacct cgtcgaggtc gatcgtcgag ccgcggtcta tgtagagcct
     2161 catctccttg tagaggtcgg gcgagccgag gccgtagatg caggagaccg tggccacgat
     2221 gatgacgtcg cggcgctcca tgagggcggc ggtggccgag agccgcatgc ggtcgatctc
     2281 ggcgttgatg tccgagtcct tctctatgta gaggtccttg gacggcacgt aggcctcggg
     2341 ctggtagtag tcgtagtagg agacgaagta ctcgacggcg ttctcgggga agaaggtctt
     2401 gaactctcgg tagagctggg ccgagagggt cttgttgtgc gagacgatca gggccggcat
     2461 ctggaggccc tcgatgacct tggccatcgt gaaggtcttc cccgagcccg tgaccccctt
     2521 cagggtctgc cagcgcaggc cctccctgac ccccgagacg agcgtgtcga tcgcctgtcc
     2581 ctggtccccc gccgggccat agggtgcgac gactttaaat ggtttcacga tgctcccccg
     2641 gccggcgcat gcgcgccgac ccgtttcact ggtcgacgcc ggtcttggcc ctgttcgaga
     2701 gggcgaggtc gacggtcatc ttcttcgagc cgtgccagta ttcgacggtg accttctgtc
     2761 cgggcttggt cgtctcgagg gccgagtaga actcggcgat cgtcgccgtc ttcatgccgt
     2821 tgaccgagac gatgatgtcg ccccccacgt tgaaggtcga gcggccgtag cgcaccgccg
     2881 tgtccccgcc cctgatcccg gcccggtcgg cgttgccgcc gcgggtcgcc tgggacacga
     2941 ggaggccggc gctcaccggc acggggtagc cgttctgctt catgtagtcg atgaggtcgg
     3001 ggaagagctg gatgggctcg atgtcgaccc atccccgcct caccatgccg tcgcggatga
     3061 gctcgggcac tacccgtttg gcggtgttga ccggcacggc gaaaccgatg ccgaccgagg
     3121 cgcccgaggg gctgtagatc atggtgttga ccccgatcat ctcgccgcgc gagttgagca
     3181 gggggccgcc cgaattgccg gggttgatcg aggcgtcggt ctggatcatg ttgcggatga
     3241 tgttggtgtc gctttccttt atcggccggc cgaggctcga gacgatgccc ctcgtgaggg
     3301 tgcggtcgaa gccgaagggg ttgccgatcg ccaggacctt ctgccccacc ttgaggcccg
     3361 aggagtcccc gtaggggatg acggtgagcc tggcgttcct gggggcgtcg aacttgatga
     3421 cggccatgtc gttctcgggg tcgacgccga ctaccttggc ctcgtagcgg gccccgtcgg
     3481 ccaggttgac gtagagcttg taggccccct tgacgacgtg gttgttcgtg aggacatagc
     3541 cccgcgagtc gatgatggag cccgagcccg tgccgccctc ctggggcacc gcctcgagga
     3601 accagttcat cgcgaccacc tcggtggtga tgttgacgac gccctcgttg aggcgctcgt
     3661 agacgtcgat gttctcgcgc tcgtcctggg tgtactcggc ggccacctcg gtctggcgga
     3721 gcctcgggtc ggcctgggcc gaggaggaca ggccagcctg cccggcggga gaggagctct
     3781 ggacgatgcc cgaccccgtc gccccgcccc agggctgcag gaggccgagc ccgaaggcaa
     3841 gcagcaatgc aaggacgacg ccgcccaagg cgaccagaag gacctgccct cggctataga
     3901 gtctcatacc cctacctcca cgaaacgaga atgggctccg gcgccctttc cgacgataga
     3961 aatatacccg aaagggatgg cagcgtaaat attcggcggg aagccaggct tcgcgtggat
     4021 cgggaggggc cccaggcagt aatataccga gatggctggg cagatcacgc acatcatcgc
     4081 gggcgagagg gcccttcgcg aggcagcgcc cgaggagggc gaagagctcc tcagggagca
     4141 ggggcctgcc ttcaggctcg gctgccaggg ccccgacatc ttctaccaca accagcgcac
     4201 gaagccctcg ggcctccatt acggggccct cgcccaccga cggggctacg gacgcctcgt
     4261 cgcgggggcc ctggcctccc tgccacccga gccgagcccg gccagacgcc aggccgaagc
     4321 ctacatcttg gggctcgcga cccacgccgc cctcgaccgc gcgacccatc ccttcatcgt
     4381 ctacttctcc ggctggcatg acccggccat ccccggcagc gaacgctacc gctcctgcca
     4441 ccccttcctc gagcgcctcc tcgacgtcgg ctacctcgcc gcctcgctcg ggcttccgcc
     4501 ggcggccttc gacatcgccg cgaaactcgg cctcgagacg ggctccacgc cggaaagcct
     4561 cggcctcccc aaggacaggg ccggggaggc cctgatagtc gcgctctgga gctccgggct
     4621 ccgctcggcc tttccccgct cgacgggcag cgatttcctc ctcgagaaaa gggtcgagaa
     4681 cgccctcctc gacgggcgcc atttctacgg gatcaccaac cccgccgtca cagccctcaa
     4741 ccccgagcgc aaggactggt tcggctatct cgacgacagg gcgggcccga ggagcgtctg
     4801 cctcgtctac ccagactccc tgcccgaagg tctggacgtc ctcaacctgg gcaaggcagc
     4861 ctgggaggag ccctcggggc tcggcacgga gcgggacgag tcctacctcg agctcgtgga
     4921 aaaggggatc catacggccg cagtggccct cggcgccctc ctgcccgccc tcgccactga
     4981 ggggcccccc ggcttcgagc tcgctgcggc gataggcgac ggcgggctct ccatcgtcga
     5041 cgccgagggc cgctcggtcg ggcccagggt ctgccgcccc ctgcccctca tggagatcat
     5101 ggagctcgag tacggccgcc gccttgaatg ggcgaaacgc cagctcggca aagcctgagc
     5161 gtcattgacc gcccctcccc gcc
//
LOCUS       DASSQV010000196         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_422138, whole genome shotgun sequence.
ACCESSION   DASSQV010000196 DASSQV010000000
VERSION     DASSQV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422138"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..469
                     /locus_tag="VFL04_03390"
     CDS             83..469
                     /locus_tag="VFL04_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HET7838780.1"
                     /translation="MGRLILRLMLGGLLLFHGVSKLVFGIAWIQEALAAARLPPIAAY
                     GVYVGEVLAPLLLILGLWSRVASLIVAFDLLVAILLVRLPAMFSMDRTGGWAMEQDFF
                     FLLSALAVFLLGPGRLALGSRGRGAA"
     gene            complement(674..2329)
                     /locus_tag="VFL04_03395"
     CDS             complement(674..2329)
                     /locus_tag="VFL04_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET7838781.1"
                     /translation="MQFPELDIRTVIGLLVIGNLGALATLVAYWRRVRGEGTGLLFMA
                     GNLVQAIAWSLLYLRGFAPELLSVQLGNSLLLCSFALEALAIAKAAGGGPRLGPAYVA
                     VTAAGVALFLLLAGTPNGRVVGSSLASVGPFALLSASVLRRRGGSALRSLIGLGSALF
                     CLIMLGRAWAGFAGGPDFGLMTPALIQSLAFIPVFLFLIVGTIGFILLLKEGADLRLR
                     ESEEQYRTLVERASEAIIIAQDERFAFVNRRMAELLGVPVQDLVGKPFVDHIHPDDRD
                     FVLANFRSRIAGGQPPDSYDLRLMGAGNRPVWTSLSATVVGWKGGPATLALLTDISTR
                     KAAEERVAQLLAEKDILLREVHHRIKNNLAVAISLLSLQSETAGGKDPATVLLDARNR
                     LQSLAQLYELLHRSGSFSEMSIRDFLSSLARELAAAFPLASALRFELELEDFALDVGR
                     LTTLGIMVNEMITNSLKHAFAGQAEPCIRITASASSGRVRLSCSDNGPGLPPGFDAKA
                     SGGLGMQIIRMLAGQLDASLEIGEGPGTTYLVEFGRALPGQGR"
BASE COUNT          380 a          786 c          893 g          379 t
ORIGIN      
        1 taatctaagg aatgggcggt tcccctgccg cccgaggagg ccggcgaaga tggccagcgc
       61 gggacatttt gatagggaag acgtgggcag gctcatcctc cggctgatgc tgggcggcct
      121 cctcctcttc catggcgtgt cgaagctcgt tttcgggata gcctggatac aggaggcgct
      181 cgcggccgcc cgccttccgc ccatcgcggc ctatggggtg tatgtggggg aggtcctggc
      241 gccccttctc ctgatccttg gcctctggtc ccgtgtcgcg tcgctcatcg tggccttcga
      301 cctcctcgtc gccatcctcc tcgtgaggct gccggccatg ttctcgatgg acaggacggg
      361 gggctgggcc atggagcagg acttcttctt cctcctgtcg gcccttgcag tcttcctcct
      421 cggcccggga cgcctcgccc ttggctcccg cgggaggggt gctgcctgag gcctgaggcc
      481 gaatccagac cccgaggcgg ggtacggacg ggggccgccg ccggaacggg ggcttgcgcc
      541 ccgggattga gttaattaat atatacctcg tgtcctctgc ctccgggcag ccggtcgtac
      601 ccgcccgggc tcgggggacc caaggcgtcc gcatcgacga cctgagcggc gccggctgga
      661 cggagttccg gacttacctc ccctgaccgg gcagggcgcg gccgaactcg acgaggtagg
      721 tcgtgcccgg gccctccccg atctccaggc tcgcgtccag ctgcccggcc agcatcctga
      781 tgatctgcat cccgagcccg cccgaggcct tggcatcgaa gcccggcggc aggcccgggc
      841 cgttgtcgga gcatgagagg cgcacgcggc cagaggacgc cgatgccgtg atccggatgc
      901 agggctcggc ctggccagca aaggcgtgct tgagcgagtt ggtgatcatc tcgttgacca
      961 tgatgcccaa ggtggtgagg cggccgacgt cgagggcgaa gtcctcgagc tcgagctcga
     1021 agcgcagggc tgaggccagg ggaaaggccg cggcgagctc cctggccagc gaactgagga
     1081 agtccctgat cgacatctcg gagaagctgc ccgagcggtg caggagctcg tagagctggg
     1141 ccagggactg cagcctgttc cgggcgtcga ggaggacggt cgcggggtcc ttgccgccgg
     1201 ccgtctccga ctggagggac agaaggctga tcgccacggc caggttgttc ttgatcctgt
     1261 ggtgcacctc ccgaaggagg atgtccttct ccgcgaggag ctgggccacc cgctcctcgg
     1321 ccgccttgcg cgtgctgatg tcggtgagga gggcgagggt ggccggcccg cccttccagc
     1381 cgaccacggt ggcagacagc gaggtccata ccggacggtt gcctgcgccc atgagcctca
     1441 ggtcgtagga gtcgggcggc tggccgccgg cgatccggga cctgaaattg gcgaggacga
     1501 agtcccggtc atcggggtgg atgtggtcga cgaagggctt tccgacgagg tcctgcaccg
     1561 ggacgcccag aagttcggcc atcctccggt tgacgaaggc gaagcgctcg tcctgggcga
     1621 ttatgatggc ctcgctcgcc ctctcgacca gggtacggta ctgctcctcg ctctcgcgca
     1681 ggcggaggtc ggccccctcc ttgaggagga ggatgaagcc gatggtgccc acgatgagga
     1741 agaggaagac ggggatgaag gccaggctct ggatgagggc cggggtcatg aggccgaagt
     1801 cggggccgcc ggcgaaaccc gcccaggccc tgccgagcat gatgaggcag aagagggcgc
     1861 tcccgagccc gatgagggac cgcagggcgc tgccccccct cctcctcagg acgctcgcgg
     1921 agagcagggc gaagggcccg accgaggcga gcgaggagcc caccacccgg ccgttgggcg
     1981 tgcccgcgag gaggaggaaa agcgcgaccc ctgcggccgt gacggccacg taggctgggc
     2041 caagcctggg cccgccgccg gccgccttcg cgatggccag ggcctcgagg gcgaagctgc
     2101 agaggaggag ggaattgccg agctggacgg acaggagttc gggcgcgaag ccgcggaggt
     2161 agaggaggga ccatgcgatg gcctggacca ggttcccggc catgaagagg aggccggtcc
     2221 cttccccgcg cactcgcctc cagtaggcga cgagggtggc cagggccccg aggttcccta
     2281 ttacgaggag cccaatgacg gtcctgatgt cgagttcggg aaactgcatg gacccctttc
     2341 cggccgagcg aagccgccgc cgcgccggcg cggcgacctc gcggcgccgt ccccgagcct
     2401 atcatgggct ttccgggctg caaagtggcg aaacacgg
//
LOCUS       DASSQV010000197         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1912996, whole genome shotgun sequence.
ACCESSION   DASSQV010000197 DASSQV010000000
VERSION     DASSQV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912996"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFL04_03400"
     CDS             <1..282
                     /locus_tag="VFL04_03400"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7838782.1"
                     /translation="GLIINELVSNALKYAFPGEASGHIRLVLERRDGREIFLGVTDDG
                     VGLPPGFDPRRDGHMGMKIVFSLAETQLRARVACESAKGLSYSIRFSEA"
     gene            complement(279..1154)
                     /locus_tag="VFL04_03405"
     CDS             complement(279..1154)
                     /locus_tag="VFL04_03405"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7838783.1"
                     /translation="MYSAIAPLVGALITLMYGVNSRFSGQVGNPVATLVIHLAGLGGI
                     SLLLLARPEEKKPGRQPFYLYLGGMVGVGTVFTSNYAFAALGASLAVALGLLGQTLSS
                     LVLDATGFLGRKRYPPTARSLPGIGLAFGGVAVLAGDWRLEPLAMLAGLASGAIPVLS
                     VLLNSELGRRKGIFRSTRTNYIMGLATSLAIVAAYRLPFAASARAVLVAGPVLALSGG
                     LMGIVVVAAMSLIFPRIPAFSATLLMFSGQALSGVLIDLVADGSFEPRKLAGSILVLS
                     GLGVNALLSRSRAPA"
     gene            1355..3319
                     /locus_tag="VFL04_03410"
     CDS             1355..3319
                     /locus_tag="VFL04_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7838784.1"
                     /translation="MLSMRLQRYLSDLMWLCMAPLLVLALYLAVNHIQVLQAQFDRDA
                     AYRVRNVTTAIDARLQAQIGALQMLAASPLIDDPRRWREFYDSAASYRVYFDGQVILA
                     DLSMRMLLNTRSPFGARLPHLPVPKGHAAVKTVLETGKPSVGDSFMGPIAGELLVSAV
                     VPVLRQGSIRYLLLGTIEARQYQALLAQVALPPGWTISLVDSIGGTMASRTSPGPKAG
                     AAPSEADKRFVAKSTLAEWSAILEMPMRYYRAPVVFELAALVVGFLSAAIISVVAGSL
                     FSRRLTRSVASIGAVPPLSLSGPSIREIEEVRERLATLEADKRENMDRYQALVDQASD
                     AFFVHGFDGRFLEVNQRACESLGYTKEELLGMSVPDIELDFDLAAAQAEWAKIEPGRQ
                     FTLRGHQRRKDGSAFPVEVRFGCSVWKGQKLFLGLARDISERVEAEERIRGSLLEKEV
                     LIRELYHRTYNNMQLIQAMMVLQLSVHPDTPLSDFVGLMTQKIEVMASVHRRLYEAGD
                     LSRIDLSAYIAELAEFVLKNHVDHGRKVQLRLERKEVQVLIDVAIPVGLVVHELLTNA
                     LEHAFSDRETGEIRIAVSRAGSGMIELVVADDGTNPNRAEASTDRTRLGLRLVDSIIE
                     EQLRGSISADHRSGSSYLIRFSDDQYMVRV"
BASE COUNT          594 a         1125 c         1118 g          524 t
ORIGIN      
        1 ggcctcatca tcaacgagct cgtgtcgaac gcgctgaagt acgccttccc cggcgaggcc
       61 tcgggccaca tcaggctcgt ccttgagcgg cgggacggac gtgagatctt cctcggcgtg
      121 acggatgacg gcgtcggcct gccccccggc ttcgacccga ggcgggacgg ccacatgggg
      181 atgaagatcg tcttcagcct cgccgagacc cagctcaggg ccagggtcgc ctgcgagagc
      241 gcgaagggtc tgtcctactc gatccggttc agcgaggcct aggctggggc ccggctccgc
      301 gacagcaggg cgttcacccc caggcccgag aggaccagga tgctccccgc gagcttcctc
      361 ggctcaaagc tcccgtcggc gacgaggtcg atgaggaccc cgctgagggc ctggcccgag
      421 aacatgagga gggtggcgga gaaggccggt atgcgaggga agatgaggct catggcggcg
      481 accaccacga tccccatgag tcctccagac agggcgagga cggggccggc caccagcacg
      541 gcgcgggccg aggccgcgaa ggggagcctg tacgcggcga cgatcgcaag gctggtcgcg
      601 aggcccatga tgtagttggt gcgggtgctc cggaagatgc ccttccgcct gccgagctcc
      661 gaattgagga ggaccgagag gacggggatg gcccccgagg cgaggccagc gagcatggcc
      721 aggggctcga gcctccagtc ccccgcgagg acggcgacgc ccccgaaggc gaggccgatc
      781 cccggcaggc tgcgcgccgt gggggggtag cgcttccggc ccagaaagcc cgtggcgtca
      841 aggacaagtg aggacagggt ctgcccgagc aggcccaggg cgaccgcgag ggaggcgccc
      901 agggcggcga aggcgtagtt gctcgtgaat accgtcccga cgccgaccat gccgcccaga
      961 tagagataga agggctgacg ccccggcttc ttctcctcgg gtctcgccag aagcaggagc
     1021 gagatgccgc cgagcccggc aaggtgtatc acaagggtgg cgacggggtt gcccacctgg
     1081 cccgagaagc ggctgttcac gccgtacatg agggttatca gtgcgcccac caggggcgcg
     1141 atcgcgctgt acatggtcca gggtcccctt gtcgtccgcc gagtatccca gaatcgtcgc
     1201 atcgccgata ggtccccggg caggggcctg ggcccccgcc cgggcaaggg ttgacagcgc
     1261 ccccatgccg ggccatagtt tgtacaatta gttacgatct cagaaagacc aaatgccttg
     1321 agtatgcatt cgcgatctcc gaaggatggc atcaatgctc tcaatgcggc tccaaagata
     1381 cctctcggac ctcatgtggc tctgcatggc gccactcctg gtcctggccc tctatctcgc
     1441 cgtcaaccac atccaggtcc tccaggccca gttcgacagg gacgcggcct accgggtccg
     1501 caacgtcacc accgccatcg acgcccgtct ccaggcccag atcggcgccc tccagatgct
     1561 ggccgcgtcg cccctcatcg acgacccgcg gcgctggcgg gagttctacg attccgcggc
     1621 gagctaccgc gtttatttcg acggccaggt gatcctggcc gatctctcga tgcggatgct
     1681 gctcaatacc cgctccccct ttggcgcgcg gctgccccat ctgcccgtgc ccaagggcca
     1741 tgcggcggtc aagaccgtgc tggagacggg caaaccctcg gtgggggaca gcttcatggg
     1801 cccgatagcg ggcgagctcc tggtctcggc cgtcgtgccg gtgctgaggc agggcagtat
     1861 ccgctacctc ctcctcggca ccatcgaggc caggcagtac caggccctgc tcgcccaggt
     1921 ggccctgccg cccggctgga cgatctccct ggtcgacagc atcggaggga cgatggcgag
     1981 ccggacatcg cccggaccga aggccggggc cgccccgagc gaagccgaca aacgcttcgt
     2041 ggcgaagtcg acgctggcgg agtggtcggc catccttgag atgccgatgc gctactaccg
     2101 ggctcccgtg gtcttcgagc tcgctgcctt ggtggtaggt ttcctctcag cggccatcat
     2161 cagcgtggtg gcgggatccc tgttctcgcg ccgcctcacc cgctcggtgg ccagcatcgg
     2221 tgccgtgcca ccgctttcct tgagcggccc cagcatcagg gagatcgagg aggtcagaga
     2281 gcggctcgcc accctcgagg ctgacaagcg ggagaacatg gaccgctacc aggccctggt
     2341 cgaccaggcg agcgacgcct tcttcgtgca cggcttcgac ggcaggttcc tcgaggtgaa
     2401 ccaaagggcc tgcgaaagcc tcggctacac aaaggaggag ctcctcggga tgtccgtccc
     2461 cgacatcgag ctggacttcg acctggcggc cgcccaggcc gagtgggcca agatcgagcc
     2521 ggggcgccag ttcacgcttc ggggccacca gagacgcaag gacggctcgg ccttccccgt
     2581 ggaggtacgc ttcggctgtt cggtctggaa gggccagaag ctcttcctcg gcctggcccg
     2641 cgacatcagc gagcgtgtgg aggccgagga gaggatcagg ggctccctcc tcgagaagga
     2701 ggtcctgatc cgggagctct accacaggac ctacaacaac atgcagctca tccaggccat
     2761 gatggtgctg cagctgagcg tgcacccgga caccccgctc tcggacttcg tcgggctcat
     2821 gacccagaag atcgaggtca tggcctccgt gcaccgccgg ctctacgagg ccggagacct
     2881 ctcccgcatc gacctctcgg cctacatcgc cgagcttgca gagttcgtcc taaagaacca
     2941 cgtcgaccac gggaggaagg tccagctcag actcgagcgg aaggaagtcc aggtcctcat
     3001 cgatgtcgcg atccccgtcg gtctcgtcgt gcacgagctg ctgacgaacg cccttgagca
     3061 cgccttctcc gaccgggaaa cgggggagat ccgtatcgcc gtctcgaggg caggctcggg
     3121 catgatcgag ctcgtggtgg ccgacgacgg gaccaatccc aaccgggcag aggccagcac
     3181 cgacaggacg aggctcggcc tcaggcttgt cgattccatc atcgaggagc agctcagggg
     3241 cagcatctcg gccgaccacc ggagcggcag ctcctatctc atcagattca gcgacgatca
     3301 atacatggtg agggtctagg catgcttcga gtcatcaccc tcctcctcgt cgaggacgag
     3361 c
//
LOCUS       DASSQV010000198         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1042209, whole genome shotgun sequence.
ACCESSION   DASSQV010000198 DASSQV010000000
VERSION     DASSQV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042209"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFL04_03415"
     CDS             <1..550
                     /locus_tag="VFL04_03415"
                     /inference="COORDINATES: protein motif:HMM:NF021501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HET7838785.1"
                     /translation="FSGLDFGTPRGLVEGAASYYLAMLSYEHFTKEYAPTIKQGISAL
                     RAAWLAGELHAKDQGQNYDYVAKIFYAKARFLYKLAVEHEQKGKEQLALVKWLGPDTD
                     KNYGYEGVLYLEALMELKYGPDEDESKRHERLVEAKRTIAKMFGLGKKSKAKPGPLVD
                     KVRDLYDRLKSEVKVEDEDDDE"
     gene            540..1409
                     /gene="truA"
                     /locus_tag="VFL04_03420"
     CDS             540..1409
                     /gene="truA"
                     /locus_tag="VFL04_03420"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7838786.1"
                     /translation="MTNRRNIRLVLSYDGTGLGGWQRQSNARSVQEELEAALATMHGH
                     PVPTIGAGRTDAGVHALGQVANFYTDIASIPAHRFVPALNSLMSEDVRVLKASDADWD
                     FHSRYDARLRRYRYFAVCGGPADPFRLRYAHQLGRRPDLVALNAMAALILGENDFTAF
                     SAAGDASENRSRFVHESSFRWEADQLVYEVAANAFLWRMVRSLVGSMLFFEEEARRAH
                     GEELGARRAQATERMRSALSSRDRRLAGPTAPARGLFLWNVEYYPSPTRPGRGDYWAE
                     REAPGGVKTASKD"
     gene            1412..2137
                     /locus_tag="VFL04_03425"
     CDS             1412..2137
                     /locus_tag="VFL04_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET7838787.1"
                     /translation="MRQGSPIRAAIFDLDGTLVDSEPLYAESDAAFLAEYGIVLDEAT
                     AASLVGIGTRDSFLLLERLFPDSPLSALPLSERIRLKDLRYLAFAAGKEILFPASAAL
                     VAELSARGIPLALASGSSPLVVGTILAELGLAPSFRIVITAMEVARGKPEPDLFLETA
                     RRLGVEPASCLVFEDSVPGVLAAAAAGMPCVALPEPRSGRDPGFAGASLVIEGGPAAL
                     EVGGLLSRLEALGLGIGDGHRPN"
     gene            complement(2228..2677)
                     /locus_tag="VFL04_03430"
     CDS             complement(2228..2677)
                     /locus_tag="VFL04_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7838788.1"
                     /translation="MDKEQALSKVSIFEKLNPKYTKGLAGICTERTFMPGEHLMKQGE
                     SGIGLFIILSGKVKVEKSDTGGHPVELAENGAGDILGEMTVFDGAPRSASVIAVTETV
                     CLVLASWEFNAFLKAHPEAALELLPIVVARFRQTNEALVGLGSIKHG"
     gene            2924..>3039
                     /locus_tag="VFL04_03435"
     CDS             2924..>3039
                     /locus_tag="VFL04_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HET7838789.1"
                     /translation="MKEKISTKAAPAAIGPYSQAIRHGDLLYVSGQIPIDPAT"
BASE COUNT          510 a         1098 c          957 g          474 t
ORIGIN      
        1 cttcagcggc ctggacttcg gcacgccgcg cggcctcgtg gagggggcag cctcctacta
       61 tctcgccatg ctctcctacg agcatttcac caaggagtac gcgccgacca tcaagcaggg
      121 catctcggcc ctccgcgcgg cctggctcgc cggtgagctc cacgccaagg atcagggcca
      181 gaactacgac tacgtggcca agatcttcta cgcgaaggcg cgcttcctct acaagctcgc
      241 cgtcgagcac gagcagaagg gcaaggagca gctcgccctc gtgaaatggc tcggccccga
      301 caccgacaag aactacggct acgagggcgt cctctacctc gaggccctca tggagctcaa
      361 gtacggcccc gacgaggacg agtccaaacg ccacgagcgc cttgtcgagg ccaagcgcac
      421 gatcgccaag atgttcgggc tcgggaagaa gagcaaggcc aaacccggcc ccctcgtgga
      481 caaggtgcgg gacctctatg acaggctcaa gtccgaggtg aaggtcgagg acgaggacga
      541 tgacgaatag gcggaacatc cgcctcgtcc tttcctacga cggcacgggc ctgggcggct
      601 ggcagcgcca gtccaacgcg cgctcggtcc aggaggagct cgaggccgcc ctcgcgacca
      661 tgcacgggca cccggtgccg accatcgggg cggggcgcac cgacgccggg gtccacgccc
      721 tgggccaggt ggccaacttc tacaccgaca tagcctccat cccggcgcat cgcttcgtgc
      781 ccgccctcaa cagcctcatg agcgaggacg tccgcgtcct caaggcctcc gacgccgact
      841 gggacttcca ttcgcgctac gacgcccggc tgaggcgtta ccgctacttc gccgtctgcg
      901 gcggccccgc cgaccccttc cggctccgct acgcccacca gctcggccgc cgccccgacc
      961 ttgtggccct gaacgcgatg gccgcactca tcctcggcga gaacgacttc actgccttct
     1021 ccgccgccgg cgacgctagc gagaaccgct cgcgcttcgt ccacgagtcg agcttccgct
     1081 gggaggcaga ccagctcgtg tacgaggtgg cggccaacgc cttcctttgg cgcatggtga
     1141 ggtccctcgt gggctcgatg cttttcttcg aggaggaggc gaggcgggcg catggggagg
     1201 agctcggagc ccggcgcgcc caggccaccg agcggatgcg gtccgccctg agctccaggg
     1261 accgccgcct cgccggcccg actgcccccg cgcggggcct cttcctctgg aacgtcgagt
     1321 actacccgag cccgaccagg cccggccgcg gcgactactg ggccgagcgc gaggcgccgg
     1381 gcggcgtgaa gacggcgagc aaggactgag cgtgaggcaa ggctccccga tccgcgccgc
     1441 catcttcgac ctcgacggga ccctcgttga ctccgagccc ctctacgccg agagcgacgc
     1501 cgccttcctc gccgagtacg gcatcgtcct cgacgaggcg accgcggcga gccttgtcgg
     1561 catcggcacg agggactcct tcctcctcct cgagcggctc ttccccgact ccccgctctc
     1621 ggccctgccc ctgtccgagc ggatacggct caaggacctg cgctacctgg ccttcgccgc
     1681 cggaaaagag atcctcttcc ccgcctcggc agccctcgtc gctgagctct cggcccgcgg
     1741 gatccccctc gccctggcct cgggctcgag ccccctcgtc gtcgggacca tcctggccga
     1801 gctgggcctc gccccgagct tcaggatcgt catcaccgcg atggaggtgg ccaggggcaa
     1861 gcccgagccc gacctcttcc tcgagacggc gaggcggctc ggcgtggagc ccgcctcctg
     1921 cctcgtcttc gaggactcgg tgccaggggt cctcgccgcc gcggccgccg gcatgccctg
     1981 cgtcgccctc cccgagccgc gctcgggtcg ggaccccggt ttcgccgggg ccagcctcgt
     2041 catcgaaggc ggccccgccg ccctcgaggt cgggggcctc ctctcccgcc tcgaggccct
     2101 gggcctcggc atcggagacg ggcaccggcc gaactgagcc ccccaacaag cttcgaggca
     2161 ccaataaaga aaaccgcccc gggtctggcc cggggcggct tcttttggtg tgacgcggcc
     2221 ttcgggatca gccgtgcttg atcgacccga gcccgaccag ggcctcgttc gtctggcgga
     2281 aacgcgcgac gacgatcggc agcagctcga gggcggcctc cgggtgggcc ttgaggaagg
     2341 cgttgaactc ccaggacgcc aggacgagac agaccgtctc ggtgacggcg atgacgctcg
     2401 cggagcgcgg ggccccgtcg aagaccgtca tctcgcccag gatgtcgccc gcgccgttct
     2461 cggcgagctc gaccgggtgg ccgccggtgt cggacttctc gaccttgacc ttaccggaaa
     2521 ggatgatgaa aaggccgatg ccgctctcgc cctgcttcat caggtgctct cccggcatga
     2581 aggtcctctc ggtgcagatc ccagccagac ccttcgtgta tttcgggttc agtttctcga
     2641 agatgctcac cttcgaaagc gcctgctctt tatccattgt gcgctccttt atccggacaa
     2701 tagggaatgg cgttccagct gtcaaggacc gcccgcggcc cgagatttgg gcgtctgcag
     2761 agccggaagg gttttgggga atggattggc aaaacgcata ttggaaggtc gatataacca
     2821 acaaaacgaa ggcagaacaa aggaaaaaat tgacaagttg catattcaga atataatcgg
     2881 cttgagggtg gcgaccgccc cctccccctg aaggaggtgg cgcatgaaag agaaaatcag
     2941 caccaaggcc gcgccggcgg cgatcggccc ctattcccag gcgatccgcc acggagacct
     3001 gctctacgtc tccggccaga tccccatcga ccccgcgac
//
LOCUS       DASSQV010000199         4699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_546494, whole genome shotgun sequence.
ACCESSION   DASSQV010000199 DASSQV010000000
VERSION     DASSQV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546494"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1555
                     /locus_tag="VFL04_03440"
     CDS             <1..1555
                     /locus_tag="VFL04_03440"
                     /inference="COORDINATES: protein motif:HMM:NF020610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HET7838790.1"
                     /translation="RNKLGLPKERIDRARVLAKRIAAPVLDFVDGHTTVTVERAALRL
                     VGADGANADGAPVPNLVVDQLKGQLEGGALAPYVNALLRSGGSVAELNAKIAGGLKVT
                     DFPMGDRAAVKRKTEELVSAGLERVAANRTRREAKLEEWKGRNSKPLLYLIVATGNIY
                     EDVKQAQAAALKGADVIAVIRTTAQSLLDYVPFGATTEGFGGTYATQENFRIMRAALD
                     EVAEREKRYIWLTNYASGLCMPEIAAMGALERLDMMLNDSMYGILFRDINMYRTFVDQ
                     KFSRMINAYAGITINTGEDNYLTTSDAFEKANTVLASQFLNERFAYAAGMPARLMGLG
                     HAMEMDPSIKNGFLYELAQAQMAREIFPEHPLKYMPPTKFMSGDIFQGHVMDSMFNFV
                     SKATDQGIHLLGMLTEAIHTPFLMDRFLGLESAKYVMNSMADFSDEVEFREGGIVKTR
                     AHQVLEETVAFMEEIAEKGLLDSIAQGLFADVKRPKDGGKGLEGLVQKGPEYWNPVEE
                     GLKKGLGLA"
     gene            1783..2526
                     /locus_tag="VFL04_03445"
     CDS             1783..2526
                     /locus_tag="VFL04_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HET7838791.1"
                     /translation="MANVIRPYGDTMDDGKVQLSFTLPVPEGPRGREAARLSVLGWGF
                     HDCEVVHSAAVSDEFSFYVAYAKTGAGLDYDQVQSAEVHGEDKVMSMDETNEYIRDKL
                     GRKVVVVGACTGFDAHTVGIDAVMNMKGYNHHFGLERYPMIEAYNLGAQVPNEKLIEF
                     ADRVKADAILVSQIVTQKDVHVENMTQFIELLEAKGERGRFVCIAGGPRISNKFAVEL
                     GYDAGFGKGTYADHVATFIAKKLVEKAQA"
     gene            2575..3246
                     /locus_tag="VFL04_03450"
     CDS             2575..3246
                     /locus_tag="VFL04_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HET7838792.1"
                     /translation="MVTKPVISAGEAAALVKKGMVVHVGGFLGCGSPDGVIAALAKLG
                     TAGLTLVCNDTAILDQKNNRVTGVAPLVVNKQFTKVVVSHIGTNAETQRQMNAGETVV
                     DLGPQGTLAERVRAAGAGIGGFLTPTGVGTEVEQGKQVITIGNKVFLLELPLSGDIAL
                     VKAKRADRAGNLQFSKTARNFNPLMATACAIVVAEVEEIVEVGEIDPDEVHTPSIFVD
                     YLVKA"
     gene            3257..3925
                     /locus_tag="VFL04_03455"
     CDS             3257..3925
                     /locus_tag="VFL04_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HET7838793.1"
                     /translation="MIEYVENKEIIAKRIARIFKSGDVVNLGIGLPTLVANYIPEDVT
                     VVLQSENGMMGLGPVPKPGEEDKDLTNAGAMPVTVLPGGCFFDSATSFAIIRGGHVDY
                     TVLGVLEVDQEGNLANYKIPGKMVPGMGGAMDLVAGSRVVIAATTHFEKSGASKLKRH
                     CTLPLTAANEVNFIVTDLGYFEVIGGKYYLLEWFKPYTPEWIQAHTDGDIVVMSNCQC
                     SDIE"
     gene            4044..4699
                     /locus_tag="VFL04_03460"
                     /pseudo
     CDS             4044..4699
                     /locus_tag="VFL04_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
BASE COUNT          902 a         1611 c         1494 g          692 t
ORIGIN      
        1 gcggaacaag cttggattgc cgaaggagcg catagacagg gcgagggtgc tggcgaagcg
       61 catcgccgcg cccgtgctcg acttcgtgga cggccacacg accgtgacgg tcgagcgcgc
      121 cgcgctgcgg ctcgtggggg ccgacggggc gaacgccgac ggggcccccg tccccaacct
      181 ggtcgtcgac cagctgaagg gccagctcga gggcggagcc ctcgcgccct acgtcaacgc
      241 cctgctgcgc tcggggggga gcgtggccga gctcaacgcg aagatcgcgg gcgggctcaa
      301 ggtcaccgac ttccccatgg gcgaccgcgc ggccgtgaag cggaagaccg aggagctcgt
      361 ctccgcgggc ctcgagcggg tcgccgccaa caggacgagg cgggaggcca agctcgagga
      421 gtggaagggc cggaactcga agcccctgct ctacctcatc gtggccacgg gcaacatcta
      481 cgaggacgtg aagcaggccc aggccgcggc cctgaagggg gccgacgtca tcgccgtcat
      541 ccgcacgacg gcccagagcc tcctcgacta cgtgcccttc ggggccacga ccgagggctt
      601 cggcggcacc tacgccaccc aggagaactt ccgcatcatg cgcgcggccc tggacgaggt
      661 ggccgagcgc gaaaagcgct acatctggct taccaactac gcctcggggc tctgcatgcc
      721 cgagatcgcc gcgatgggcg cgcttgagcg cctcgacatg atgctgaacg actcgatgta
      781 cgggatcctc ttccgcgaca tcaacatgta ccggaccttc gtcgaccaga agttcagccg
      841 catgatcaac gcctacgccg gcatcaccat caacaccggc gaggacaact acctcacgac
      901 gagcgacgcc ttcgagaagg ccaacacggt gctcgccagc cagttcctca acgagcgctt
      961 cgcctacgcc gcgggcatgc ccgcccggct catgggcctc ggccacgcga tggagatgga
     1021 cccgagcatc aagaacggct tcctctacga gctcgcccag gcccagatgg ccagggagat
     1081 cttccccgag caccccctca agtacatgcc gcccaccaag ttcatgtcgg gcgacatctt
     1141 ccagggccac gtgatggact cgatgttcaa cttcgtgtcc aaggccacgg accagggcat
     1201 ccacctcctc ggcatgctca ccgaggcgat ccacaccccc ttcctcatgg accgcttcct
     1261 cggcctcgag tcggcgaagt acgtgatgaa ctcgatggcc gacttctccg acgaggtcga
     1321 gttccgcgag ggcggcatcg tgaagacgag ggcccaccag gtcctcgagg agacggtcgc
     1381 cttcatggag gagatcgccg agaagggcct cctcgactcg atagcccagg gcctcttcgc
     1441 cgacgtgaag cggcccaagg acgggggcaa gggcctcgag ggcctcgtgc agaaggggcc
     1501 ggagtactgg aacccggtcg aggaagggct caagaagggc ctgggcctgg cataaccggg
     1561 acaggatggc ggccaagatg aagaaaaccg cagaggcgca gagacgcaga gagaagaagg
     1621 aggggaggag aatatgagag agaatttgaa ggattcctca attccccctc tctctctatc
     1681 actctatcac tctatcacaa tctctcttct ctgcgtcttc ctctgcgcct ctgcgtctct
     1741 gcggttctgt ctctctactt tgattttcga acgaggtgaa atatggctaa cgtgatccgt
     1801 ccctacgggg acaccatgga cgacggcaag gtccagctct ccttcaccct gcccgtgccc
     1861 gaggggccga ggggcaggga ggccgcgagg ctctccgtgc tcggctgggg cttccacgac
     1921 tgcgaggtgg tccactcggc cgccgtctcc gacgagttct ccttctacgt cgcctacgcc
     1981 aagaccggcg cggggctcga ctacgaccag gtccagtcgg ccgaggtcca cggcgaggac
     2041 aaggtcatgt ccatggacga gaccaacgag tacatccgcg ataaactggg gcgcaaggtg
     2101 gtcgtggtcg gcgcctgcac cggcttcgac gcccacacgg tcggcatcga cgccgtcatg
     2161 aacatgaagg gctacaacca ccacttcggg ctcgagcgct acccgatgat cgaggcctac
     2221 aacctcggcg cccaggtccc caacgagaag ctcatcgagt tcgccgacag ggtgaaggcc
     2281 gacgcgatcc tggtgtccca gatcgtcacc cagaaggacg tccacgtcga gaacatgacc
     2341 cagttcatcg agctcctcga ggccaagggc gagcgcggcc gcttcgtctg catcgccggc
     2401 ggccccagga tctcgaacaa gttcgccgtc gagctcggct acgacgcggg cttcggcaag
     2461 ggcacctacg cagaccacgt cgcgaccttc atcgcgaaga agctcgtgga gaaggcccag
     2521 gcctgagggc tgccggcgtg aagctcttat agtaccgttg tctggaggta atacatggta
     2581 acaaaacccg taataagcgc cggcgaggcc gcggccctcg tcaagaaggg catggtcgtc
     2641 cacgtgggcg gcttcctcgg ctgcggctcc ccggacgggg tcatcgccgc cctggccaag
     2701 ctcggcacgg ccgggctcac cctggtctgc aacgacaccg ccatcctcga ccagaagaac
     2761 aacagggtca ccggcgtggc ccccctggtc gtcaacaagc agttcacgaa ggtcgtcgtg
     2821 tcccacatcg gcacgaacgc cgagacccag aggcagatga acgccggcga gaccgtcgtc
     2881 gacctcgggc cccagggcac cctagccgag cgggtcaggg ccgcgggggc cggcatcggc
     2941 ggcttcctca cccccaccgg ggtgggcacc gaggtcgagc agggcaaaca ggtcatcacc
     3001 atcgggaaca aggtcttcct gctcgagctc cccctgagcg gcgacatagc cctcgtgaag
     3061 gccaagcgcg ccgacagggc cggcaacctc cagttctcga agacggcgcg caacttcaac
     3121 cccctcatgg ccacagcctg cgccatcgtg gtggccgagg tcgaggagat cgtcgaggtg
     3181 ggcgagatcg accctgacga ggtccacacc ccctcgatct tcgtcgatta cctggtgaag
     3241 gcctaaggag ggcgggatga tcgagtacgt cgagaacaag gaaatcatcg cgaagcgcat
     3301 cgcgcgcatc ttcaagtcgg gcgacgtggt caacctcggc atcggcctgc ccaccctcgt
     3361 cgccaactac atccccgagg acgtcaccgt cgtcctccag tccgagaacg gcatgatggg
     3421 cctcggcccc gtgcccaagc cgggcgagga ggacaaggac ctcacgaacg ccggagccat
     3481 gcccgtcacc gtcctgcccg ggggctgctt cttcgactcg gccacgagct tcgcgatcat
     3541 ccgcggcggc cacgtcgact acaccgtcct cggggtcctc gaggtcgacc aggagggcaa
     3601 cctcgccaac tacaagatcc ccggcaagat ggtccccggc atgggcgggg ccatggatct
     3661 cgtggcgggc tcccgcgtcg tgatcgccgc aacgacccat ttcgagaagt ccggcgcctc
     3721 caagctcaag cgccactgca ccctgcccct gaccgctgcg aacgaggtga acttcatcgt
     3781 caccgacctc ggctatttcg aggtcatcgg cggcaagtac tacctcctcg agtggttcaa
     3841 gccctacacc cccgagtgga tccaggccca caccgacggc gacatcgtcg taatgagcaa
     3901 ctgccagtgc tccgatatcg agtagggcgt ttccgaagga cagcggaaaa ccgtatcatc
     3961 aaaggagaga caccgtgaaa gacgcataca tcatcgacgc ggctcgcacc gcgatcggca
     4021 acttcggcgg ggccctggcg ggcgtgagcg cggccgagct cgggaggacc gcggtccgcg
     4081 ccgtcctgga ccgggccaag gtcgggaacg acgaggtcga cgagctcgtc ctcggctgcg
     4141 tcctccagtc gggcctgggc cagaacgtcg cgcggcaggt cctcgtgtag gcgggcctcc
     4201 ccgaggccag gacggcctcg accgtgaaca tggtctgcgg ctcggggctc cgggccgtcg
     4261 ccgcggccgc ccaggccatc agggcggggg acgcctcact cctcgtcgcc ggcgggaccg
     4321 agagcatgag ccaggccccc tacctcctca agcaggcccg caccggctac cgcatgggcg
     4381 acggcagcct cgtggactcg atgatctgcg acgggctctg ggacgccttc aacgactacc
     4441 acatgggcct gaccgccgag aacctcgccg ccaagtacgg cctcacgagg gccgagcagg
     4501 acgccttctc ggccgcctcg cagaacaagg ccgagaaggc catggccgag ggccgcttca
     4561 gggacgagat cgtcccggtc tccatccccc agcgcaaggg cgatccgatc atcttcgacc
     4621 gcgacgagtt tccccgcgcc ggggtcacgg ccgagtccct ggccaagctc aagcccgcct
     4681 tcaagggcga cgggacggt
//
LOCUS       DASSQV010000200         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2164073, whole genome shotgun sequence.
ACCESSION   DASSQV010000200 DASSQV010000000
VERSION     DASSQV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164073"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFL04_03465"
     CDS             <1..289
                     /locus_tag="VFL04_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET7838794.1"
                     /translation="SSLVTNLSSERIIDLYEEVSINEKTTLIHEFLESKGSFSFTDLV
                     TRPRSTMDIVCAFLAILEAVKYRIISIYQHKLFGDIMIRGYDKREDHGNQA"
     gene            273..809
                     /gene="scpB"
                     /locus_tag="VFL04_03470"
                     /pseudo
     CDS             273..809
                     /gene="scpB"
                     /locus_tag="VFL04_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            904..1692
                     /locus_tag="VFL04_03475"
     CDS             904..1692
                     /locus_tag="VFL04_03475"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET7838795.1"
                     /translation="MPTDDAGQGSQRLQAYLAHAGVASRRASERIILEGRVKVNGSVV
                     TELGTKVRPTDRIELDGKPLGAAERKRYILLNKPPGYLCAMSDPEGRALAVDLIRKEV
                     SERVYNVGRLDQWSAGLVLFTNDGDLAAILVHPSGGVDKEYDIVADAPLDEGFFEGFR
                     KGVTIDGVFYKALAVERTGPSEARVVLAEGKNREIRRLLEAFGRKALQLRRVRIGPLS
                     IQGLQEGGFRELSPAEVEALYSYGGRARQGLPLPPDRSSGPPRH"
     gene            1709..>2251
                     /gene="cmk"
                     /locus_tag="VFL04_03480"
     CDS             1709..>2251
                     /gene="cmk"
                     /locus_tag="VFL04_03480"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET7838796.1"
                     /translation="MIVAIDGPAGSGKSTIARMIAESLGFTYVNSGNLYRAMTLRALN
                     EGISLDAREAVTQCSRAAEIVYRDGRLHLDGRDAEDELHSARVDALVAQLSAIPEVRE
                     IVNALVRRIADDLDSVVEGRDMTTVVFPDAELKFFLDASPEARAQRRFSQGTSGASLE
                     EIKANIEMRDAIDRGKSVGSL"
BASE COUNT          429 a          719 c          724 g          379 t
ORIGIN      
        1 ttcctcgctg gtcacgaacc tgtcctcgga gcggataatc gacctgtatg aggaagtctc
       61 gataaacgag aagacgaccc tcatccacga gttcctcgaa tcgaagggct ccttcagctt
      121 caccgatctc gtcacccgac ccaggtcgac gatggacatc gtctgcgcct ttctggccat
      181 cctggaggcc gtcaagtaca ggatcatctc gatataccag cataagctgt tcggcgacat
      241 catgatccgg ggctacgaca aacgggagga ccatggaaat caggcttgaa cgggaggcgg
      301 ccatcctcga gaccgtcctg ttcctagagt ccgaggcctt ggacgaggcc gccttgtccc
      361 ggatcaccgg gctttcgcgc gatgtcgtcg agaaggcgct ggaactgctg gtggaggagc
      421 actccgcccc atcccatggc ctcgagcccc tccgatcagg gggcggctgg atcctcgctc
      481 ccaaggccga gctctgggag tccctgaagg agcgatacgg gaagaagaac gattcacggc
      541 tgtcccgcgc cgccctcgag accctttcca taatcgccta ttcccagccc atcacgaggg
      601 cagagatcga ggccatccgc ggcgtctcgg cagacggaat gatgcggttc ctgctagaga
      661 aggagttcat caaagaggtc gggaagaagg aagcccccgg gaggcctgtc cagtacggga
      721 ccacgaagga cttcctcaag tacttccggc tcgggagcat cgccgacctg cccaggctcg
      781 acgagctgga gcgcgagcgc ttcgggcttg agaccgcaaa ggccgagccc gagccagcgc
      841 agccctcggg cgagacgggg agctcggatc gtgatactcc cgcccagact gaggtcggcg
      901 gcgatgccga ctgacgacgc aggccagggg tcccagcgtc tgcaggccta tctagcccat
      961 gcaggcgtcg catcgcggcg ggcctctgag cggatcatcc tcgagggtcg tgtcaaggtc
     1021 aacggctcgg tggtcactga gctggggacg aaggtgcgcc ccaccgaccg gatcgagctc
     1081 gatggtaagc cgctcggggc cgccgaacgc aagcgctaca tcctgctcaa caagcctccg
     1141 ggctacctct gcgccatgtc cgatcccgag ggccgcgccc tcgccgtgga cctgatccgc
     1201 aaggaggtca gcgagcgggt ctataacgtc ggacgcctcg accagtggtc ggcagggctc
     1261 gtcctgttca cgaacgacgg cgaccttgcc gctatcctcg tccatccctc gggcggagtc
     1321 gacaaggaat acgacatcgt agccgacgcc cccctcgacg aggggttctt cgagggcttc
     1381 cgcaagggcg tgacgatcga tggggtcttc tataaggccc tggccgtcga gaggacgggg
     1441 cccagtgagg ctcgagtcgt cctggccgag ggcaagaaca gggagatcag gcgtctgctc
     1501 gaggcctttg gccgaaaggc cctccagctc cgcagggtaa ggatcggccc actgtccatc
     1561 cagggactgc aggagggcgg cttccgcgag ctttcgcccg ccgaggtcga agccctgtat
     1621 tcctatgggg gccgcgccag acagggcctc cctcttccgc ccgaccggag ttcgggtccg
     1681 cctcgacact agcctcggag gattccccat gattgtcgcc atcgacggac cagcaggttc
     1741 gggcaagagc accattgccc gaatgatcgc cgagtctctc ggcttcacct atgtcaactc
     1801 gggcaacctc taccgggcga tgaccctgcg ggcgctcaat gagggcatct ccctggatgc
     1861 gcgggaagcc gtgacccaat gctcccgggc ggcagagata gtctacaggg atggccgtct
     1921 acatcttgac ggaagggacg ccgaggatga gctccactcc gcccgggtcg acgcccttgt
     1981 agcccagctt tcggccattc ccgaggtgcg ggagatagtc aacgctctgg tccggcgcat
     2041 cgccgatgac ctcgattcgg tggtggaggg cagggacatg acaacggtgg tcttccccga
     2101 tgccgagctc aagttcttcc tcgatgcgag tcccgaggcc agggcccagc gccgtttctc
     2161 ccaggggacg agcggcgcaa gcctggagga gatcaaggcg aacatcgaga tgagggacgc
     2221 gatcgacagg ggcaagtccg ttggcagcct a
//
LOCUS       DASSQV010000201         3507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_175679, whole genome shotgun sequence.
ACCESSION   DASSQV010000201 DASSQV010000000
VERSION     DASSQV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175679"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1913
                     /locus_tag="VFL04_03485"
     CDS             <1..1913
                     /locus_tag="VFL04_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET7838797.1"
                     /translation="ARAHRDIRSLTDSFYKDPELRSMLQSDEPTLRDGRTVLALRVNF
                     KGRVKGIVHEVSSSGQTVFVEPEELIQKNNELVQEEARYRMELARILREATERLHPES
                     DAIAGARAVLAVIDACWARARHARVGGLVMARDLAEGFLLRSARHPLLGPRAVPIDVE
                     LPEGTRTLIVTGPNTGGKTVTLKTIGLLALMNQFGLGVPAASESGFAVFDAVHADIGD
                     EQSIDQSLSTFSGHMRVIGEIATEAQGRSLVLLDELGSGTDPEEGCAIAMSLLDYFIG
                     RGCLTVLTTHHGILKNYGYSRPGCLNASMDFDAASLSPTYRILMGIPGESRALEVASK
                     NGLSAPIVEGARKDLAEERADVSELIRGLTEKHRQLEDLEADRKKRLRAAMEEQRKAD
                     LGKLRLRQAEIELRERGVGDLKRLLSESRKTLENLVRDLREAGVATDKTKEVKDFLAS
                     LAGAVEGADQRLEEIRAEGPAEPGAEEASPTGQAGPLAEGSHVLVGPARKRGVIVRRA
                     RKGHWVVETDSIRLTLPETELRAVGEGPQSKPLVQVELAPRGEGGKVNAVFELDLRGQ
                     RLVEALDAVEKQVDAASLQGLSLFSIIHGTGEGVLGKGIQEYLRSHPSVADYHFARPE
                     EGGWGKTVVRLK"
     gene            complement(1924..3054)
                     /locus_tag="VFL04_03490"
     CDS             complement(1924..3054)
                     /locus_tag="VFL04_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HET7838798.1"
                     /translation="MGGRGYGPGRVLVIAEIGTGHGGDPAKAAELCAAAAEAGADCAK
                     FQCVIASEIIHPKTGVVPLPGGDIALFERFRELELDEGFYAAAKEAAEARGLVFLCTP
                     FGLESARLLRRLGVAAMKVASPELNHLGLLDELASYGLPTILSSGVSTLADIETALAR
                     LAPPSGEGPAGGVAPGLALLHCVTAYPAPAEDYNLSLLGAYASLFGVPVGVSDHSLDP
                     VLVPALSVAAGGVIVEKHICLSREDPGLDDPIALPPADFARMTASVRAAQTSPAADTV
                     EGLSSAYGRLRVERVMGDGVKRLAPSERANYGRTNRSIHALRDIAEGEAFGPGNLALL
                     RTEKVLKVGLAPALLDAISGRRARRAIPSGEGIEWEDVGDLG"
     gene            3158..3397
                     /locus_tag="VFL04_03495"
     CDS             3158..3397
                     /locus_tag="VFL04_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838799.1"
                     /translation="MEVLYCDVCKKAVENPIPQRSFFHYADIEICEPCRDELNLAVKY
                     TVRGKKPFDYSWFNELELKILRDGVARGKISPAKR"
BASE COUNT          575 a         1206 c         1244 g          482 t
ORIGIN      
        1 tagccagggc ccaccgcgac atcagatccc tcacggactc cttttacaag gaccccgagc
       61 tgaggtccat gctccagtcc gacgagccca cactgcgcga cggcaggacc gtcctcgccc
      121 tcagggtcaa cttcaagggc cgggtcaagg gcatcgtgca cgaggtctcc tccagcgggc
      181 agacggtctt cgtcgagccc gaggagctca tccagaagaa caacgagctc gtgcaggagg
      241 aggcgcgcta ccgcatggag ctcgcgcgga tcctgcgcga ggcgacggag cggctccatc
      301 cggagtccga tgccatagcc ggggccaggg cagtcctcgc cgtcatcgac gcctgttggg
      361 cgagggcgag gcacgcgagg gtgggggggc tggttatggc ccgggacctc gccgagggct
      421 tcctcctccg ctcggcccgc caccccctcc tcggcccccg cgccgtgccg atcgacgtgg
      481 agctgcccga gggcacgcgg accctcatcg tcacggggcc caacacgggg ggcaagaccg
      541 tcaccctgaa gacgatcggc ctcctcgccc tgatgaacca gttcggcctg ggcgtgccgg
      601 cggcctcgga gtcgggcttc gcggtcttcg acgccgtcca cgccgacatc ggggacgagc
      661 agtcgatcga ccagtccctc tcgaccttct ccggccacat gcgggtcatc ggcgagatag
      721 ccacggaggc ccagggccgg agcctcgtcc tcctcgacga gctcggctcg ggcaccgacc
      781 ccgaggaggg ctgcgccatc gccatgagcc tcctcgacta cttcataggc cgcggctgcc
      841 tcaccgtcct cacgacccac cacgggatcc tcaagaacta cggctattcg aggccgggct
      901 gcctcaacgc ctcgatggac ttcgacgcgg cgagcctctc gccgacctac cgcatcctca
      961 tggggatccc cggcgagagc cgggccctcg aggtggcctc gaagaacggc ctcagcgccc
     1021 ccatcgtcga gggcgcgcgc aaagacctcg ccgaggagag ggccgacgtc agcgagctca
     1081 tccgcgggct gacggagaag caccgccagc tcgaggacct cgaggccgac cggaagaagc
     1141 ggctcagggc ggcgatggag gagcagcgca aggccgacct cggcaagctc aggctccgcc
     1201 aggccgagat cgagctccgc gagcgcgggg tgggcgacct caaacgcctc ctctccgaga
     1261 gccgaaagac cctcgagaac ctggtcaggg acctcaggga ggccggggtg gccaccgaca
     1321 agaccaagga ggtcaaggac ttcctcgcga gcctggccgg agccgtcgag ggcgcagacc
     1381 agaggctcga ggagataagg gccgagggcc cggccgagcc cggggccgag gaggcttcgc
     1441 ccacgggcca ggcgggccct ctagccgagg gcagccacgt cctcgtcggc cccgcgcgga
     1501 agcggggcgt catcgtgcgg agggccagga agggccattg ggtggtcgag accgactcga
     1561 tccgcctcac cctgcccgag accgagctca gggccgtggg cgaggggccc cagtccaagc
     1621 cccttgtcca ggtcgagctc gcccctcgcg gcgagggcgg gaaggtgaac gccgtgttcg
     1681 agctcgacct gcgcggccag aggctggtgg aggccctcga cgccgtggag aaacaggtcg
     1741 acgcggcgag cctccagggc ctctccctct tctcgatcat ccacgggacc ggcgagggcg
     1801 tgctgggcaa ggggatccag gagtacctgc gctcccatcc cagcgtcgcc gactaccatt
     1861 tcgcccggcc cgaggagggc ggctggggga agaccgtggt gcggctcaaa tagggcgccc
     1921 ctcctagccg aggtcgccca cgtcctccca ttcgatgccc tcgcccgagg ggatcgccct
     1981 cctcgccctt ctccccgata tggcgtcgag gagggcgggg gccaggccca ccttgaggac
     2041 cttctcggtc cggagcaggg ccaggttgcc gggcccgaag gcctcgccct cggctatgtc
     2101 gcgcaacgcg tggatcgacc ggttggtccg cccgtagttg gcccgctcgc tcggggcgag
     2161 gcgcttgacc ccgtcgccca tgaccctctc gaccctcagc ctgccgtagg ccgaggagag
     2221 cccctccacc gtgtccgcgg cgggggatgt ctgggcggcc ctgaccgagg ccgtcatccg
     2281 ggcgaaatcg gcggggggca gggctatggg atcgtccagc ccggggtcct cccgggagag
     2341 gcagatgtgc ttctcgacta tgacgccccc ggccgccacg gagagggcgg gcacgaggac
     2401 ggggtcgagg gagtggtcgg agacgcccac ggggacgccg aagagggagg cataggcccc
     2461 aaggagggag aggttgtagt cctcggcggg ggcggggtag gcggtgacgc agtggaggag
     2521 ggcgaggcca ggggccacgc ccccagccgg cccctctccg gaggggggtg cgaggcgggc
     2581 cagggccgtc tcgatgtcgg ccagggtcga gaccccgctc gagaggatcg tggggaggcc
     2641 gtaggaggcg agctcgtcga ggaggccgag gtggttgagc tcggggctcg cgaccttcat
     2701 ggcggccacc ccgaggcggc ggaggagcct cgcgctctcg aggccgaagg gggtgcagag
     2761 gaagacgagg ccgcgggcct cggcagcctc cttggccgcg gcgtagaagc cctcgtccag
     2821 ctcaagctcc ctgaagcgct cgaagagggc gatgtcgccc ccggggaggg ggacaacgcc
     2881 cgtcttgggg tggatgatct cgctggcgat cacgcactgg aacttggcgc agtcggcccc
     2941 ggcctcggcc gctgcggcgc agagctcggc ggccttcgcc ggatcgccgc cgtggcccgt
     3001 gccgatctcg gcgatgacga ggaccctgcc gggcccgtag cctctgccgc ccatcgtgaa
     3061 tacgcctggc atcgctcccc ctgtttacag cttgctttgt ccccttcgat acgctatcat
     3121 aatcctccga gagagacaaa cgcaaaggag ctgtgtgatg gaagtcctct attgcgatgt
     3181 ttgcaagaag gcagtcgaaa acccgatccc ccagcggtcc tttttccact acgccgatat
     3241 cgagatctgc gagccctgca gggacgagct caaccttgcg gtgaaataca cagtgcgcgg
     3301 aaagaagccc ttcgactatt cctggttcaa cgagctcgag ctcaagatcc tccgcgatgg
     3361 agtggcccgc ggcaagatca gcccggccaa gcgctagggc tccgggctga cctcgcccca
     3421 gaccgacccc gcccggggcc ggttgggcag accgacagga tagggccgcc atggggcggc
     3481 ccatcccgtt ttcgggggga gtcgccc
//
LOCUS       DASSQV010000202         2796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1541133, whole genome shotgun sequence.
ACCESSION   DASSQV010000202 DASSQV010000000
VERSION     DASSQV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541133"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFL04_03500"
     CDS             complement(<1..278)
                     /locus_tag="VFL04_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838800.1"
                     /translation="MKSRLLLALALQRLEFLRSDEKLLVWDLIDDETRLAVLSLRDVE
                     TIVCRRLEGRRWSPATLLAEAERDALYLERIGARYLHFDDPAYPPLLR"
     gene            complement(407..1396)
                     /locus_tag="VFL04_03505"
     CDS             complement(407..1396)
                     /locus_tag="VFL04_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7838801.1"
                     /translation="MGDLVGRYRAYLLAARHRSPLTAEVYLREAVLLESWLSDRGLAF
                     ETATSADILGFLVWRQGGGSAARTETRTGSTSRGRPAGAEAASRPGTRDQRDAQDLGL
                     SRKTMARVVSSVHALYKVMRLEGSRLDDPSELIETPKQDRQLPSVLAPEEVDRFLATI
                     DTASPRGLRDRALFELIYSCGLRISEAAALTFDQLYLEERVIRVVGKRRKERLVPFGD
                     AAASWLKRYIDEGRPALAKGRTDRVFLNQAGKGISRKGIWKRFDGFRDASGLDAKVHT
                     FRHSFATHLLAGGVDLRTVQELLGHSDISTTQIYTHIDPESLQSYHRDYFPRK"
     gene            complement(1398..2570)
                     /gene="ftsZ"
                     /locus_tag="VFL04_03510"
     CDS             complement(1398..2570)
                     /gene="ftsZ"
                     /locus_tag="VFL04_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET7838802.1"
                     /translation="MTIEMVDDRAVSPTVIKVIGAGGGGSNAVNRMIMAGLRNVQFIV
                     ANTDIQALNLSQAQTRLGLGTKVTHGLGAGGKPEVGEKAALEDRDAIAEALKGADMVF
                     ITAGMGGGTGTGSAPIIAQVARDLGALTVGVVTRPFDFEGRVKSRIAGEGIEKLRQAV
                     DTLIIIPNQYLLKVVERRTPIKQAFMLADDVLRQGVQGISDLITIPGDINIDFADVRT
                     VMQGQGDAIMGIGFASGDNRAVEAATKAINNPLLEDARIEGAKNILVNVTGGEDLSLT
                     EYEEIVNLITESSDPDALVIPGIVSDPSIGDEVRVTVIATGFNRGARPASPVREARKG
                     FEDYMSPDEWKRITDSPPPARGNAKQYLQGRNEEEDELEIPTVLRDRRFTGEKNEA"
BASE COUNT          373 a          930 c          997 g          496 t
ORIGIN      
        1 tccctcagaa gagggggata ggccggatcg tcgaagtgca gataccgggc cccgatgcgc
       61 tcaagataga gggcgtcgcg ctcggcctcc gccagcaggg tggcggggct ccatcgcctg
      121 ccctccaggc gacggcagac gatcgtctcc acgtcgcgta gggaaagtac cgcgagccgc
      181 gtctcatcgt caatgagatc ccagaccagg agcttctcgt cgctcctcag gaactcgagc
      241 cgctggaggg cgagggcgag gaggagccgg ctcttcatgc gcttcgcctc ctgggggatc
      301 tgggtccgga gccccgcggc cccgaggcgg acggcgcttc caagcccgcc tccttccctc
      361 caacgccctt gcgatgcgtc ggcgattgtg gccccgcctg ccgtcctcat tttcgcggga
      421 agtagtccct gtggtaggac tgcagtgact cgggatctat gtgggtatag atctgggtcg
      481 tggatatgtc ggagtggccg aggagctcct ggaccgtcct caggtcgacc ccgcccgcca
      541 ggaggtgggt ggcgaagctg tggcggaagg tgtggacctt cgcgtcgagg cccgaggcgt
      601 cccggaaacc gtcgaagcgc ttccagatcc ccttccgcga gatgcccttg cccgcctggt
      661 tgaggaagac cctgtcggtc ctgcccttgg cgagggcggg acggccctcg tctatgtagc
      721 gcttgagcca ggacgcggcc gcgtccccga aggggacgag cctctccttc ctcctcttgc
      781 cgacgaccct gatcacccgc tcttcgagat agagctggtc gaaggtgagg gcggcggcct
      841 cggagatcct gagcccgcag gagtagatga gctcaaagag ggcccggtcg cgcaggcccc
      901 tcgggctcgc cgtgtcgatg gtggcgagga agcggtcgac ctcctcgggg gcgaggaccg
      961 aagggagctg ccggtcctgc tttggggtct cgatgagctc gctcgggtcg tcgagccggc
     1021 tgccctcgag cctcatgact ttgtagaggg cgtggacgct cgagaccacg cgggccatgg
     1081 tctttcgcga caagcctaga tcctgtgcat cgcgctggtc ccgcgtgccg ggccgcgagg
     1141 ctgcctcggc ccctgcgggc ctgccgcggg acgtggagcc cgtcctcgtc tcggtcctgg
     1201 cggcgctccc tccgccctgg cgccagacga ggaagccgag gatgtcggcg ctggtggcgg
     1261 tctcgaaggc gagccccctg tcggagagcc aggactcgag gaggacggcc tcgcggaggt
     1321 agacctcggc ggtgaggggt gagcggtgcc gcgcggcgag gaggtaggcg cggtagcggc
     1381 cgaccaggtc tcccacccta ggcctcgttc ttctcgcccg tgaagcgcct gtccctgagg
     1441 acggtcggga tctcgagctc gtcctcctcc tcgttgcggc cctggaggta ctgcttggcg
     1501 ttgccccgcg cggggggcgg gctgtcggtg atccgcttcc actcgtcggg ggacatgtag
     1561 tcctcgaagc ccttgcgcgc ctcgcgcacg ggcgaggcgg gcctcgcgcc gcggttgaag
     1621 ccggtggcga tcacggtgac cctcacctcg tcgccgatcg aggggtcgga gacgatgccg
     1681 gggatcacga gggcgtcggg gtctgagctc tcggtgatga ggttgacgat ctcctcgtac
     1741 tcggtgagcg agaggtcctc gcccccggtg acgttcacga ggatgttctt cgcgccctcg
     1801 atcctggcgt cctcgagcag ggggttgttg atggccttgg tggcggcctc gacggccctg
     1861 ttgtccccgc tcgcgaagcc gatgcccatg atcgcgtcgc cctggccctg catcaccgtg
     1921 cggacgtcgg cgaagtcgat gttgatgtcg ccggggatgg tgatgaggtc ggagatgccc
     1981 tggacgccct gcctgagcac gtcgtcggcg agcatgaagg cctgcttgat cggggtgcgg
     2041 cgctcgacca ccttgaggag gtactggttc gggatgatga tgagggtgtc cacagcctgg
     2101 cgcagcttct cgatgccctc gccggcgatg cgggacttga ccctgccctc gaagtcgaag
     2161 ggccgggtga cgacgccgac cgtgagcgcg ccgaggtcgc gggccacctg ggcgatgatg
     2221 ggggccgagc ccgtgccggt gccgccgccc atgcccgcgg tgatgaagac catgtcggcc
     2281 cccttcaggg cctcggcgat cgcgtcgcgg tcctcgaggg ctgccttctc ccccacctcg
     2341 ggcttgccgc cggcgccgag gccgtgggtg accttggtgc cgaggccgag cctcgtctgg
     2401 gcctgagaga ggttcagggc ctggatgtcg gtgttggcga cgatgaactg cacgttgcgc
     2461 agcccggcca tgatcatgcg gttcacggcg ttggatccgc cgccgcccgc ccctattacc
     2521 ttgatgacgg tggggctcac cgccctgtcg tcgaccatct cgatcgtcat gttcccctcc
     2581 cctttctatc gacggcgcgt tccccaacgc gcccctgtcc catcctagaa gaatcccttc
     2641 ctgaaccagt ccgagagccg gccgagggcc tccctcgccg ggccctgctt gccgccccgc
     2701 tcaggcccgc gctgggcctg cctgagctcg ggctggatcg actccgcccc gagcaggacg
     2761 aggcccaccc ccgtcgcgaa gagggggctg cggtac
//
LOCUS       DASSQV010000203         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2413165, whole genome shotgun sequence.
ACCESSION   DASSQV010000203 DASSQV010000000
VERSION     DASSQV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2413165"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="VFL04_03515"
     CDS             <1..1793
                     /locus_tag="VFL04_03515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7838803.1"
                     /translation="PGVMRDSLLRDMLDAVSEGVYFVDLERRITYWNSGAERITGFRA
                     EEVIGSRCSDNILRHVDEAGKELCAGDCPLSIAMASGAKQQANVFLHHRDGHRVPVAV
                     RGIPYFDEKEAAVGAIEVFTDRSERLAILAELQELRKENLSDSLTGLGNRRYFDIRLE
                     PLLAALRGGGGPFGLLLIDIDHFKQMNDDYGHATGDRILRMVAWTLANAVRRGDVSVR
                     WGGDEFVVLAPKATPMVLGALAERIRALVASGWLSLDGGRSVSVTVSVGGAISAAGDD
                     SDALVRRADLLLYRSKEGGRNRATIEGEPMGATTGAATTGAAEEAVPESGGGGMLELG
                     AVQETLMMPLWGRAHETRKALPLLRDEAAAAIVEGIDYDFTRIAKRVNPLSRAGWIAR
                     AIYFDEAIRSFLREHERAAVVNLGCGLDTSFDRVDDGKAHWYELDLPDVIDLRRRFIA
                     EVERRVFIADSALGSAWYDRIEKGSSVLILIAGVIYYFEEAELRCLFREIRRRLGRAE
                     LIFDYASKRGMRAANRSVIGKAGMGDEARLKWGIDELRELEAWDCGLRILDDRPIFRD
                     HRKRYPPSRRLGMALSDALGIMSLARIEIA"
     gene            complement(1808..2242)
                     /locus_tag="VFL04_03520"
     CDS             complement(1808..2242)
                     /locus_tag="VFL04_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HET7838804.1"
                     /translation="MKLVSACLLGIKCNYRAEGKAEAGLVAEFAQGGLFPVCPEILGG
                     LGCPRHAAEIVGGGGAEVLRGEAKVLDSDGEDLTSEFLKGAYAVLAIARSLGAEEAIL
                     IEKSPSCGCGWIFDGSFSHRLIEGDGVTAALLKQHGIRVRVG"
     gene            complement(2261..>2493)
                     /locus_tag="VFL04_03525"
     CDS             complement(2261..>2493)
                     /locus_tag="VFL04_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838805.1"
                     /translation="LDEDLRHSLGEELRRIQRRTGVTAILVSHSRSELERLCDGILEL
                     EEGRTSGARDSSRSEALPAWWGGAIVGPRRGG"
BASE COUNT          395 a          871 c          862 g          365 t
ORIGIN      
        1 agcccggcgt catgcgcgac agcctgctca gggacatgct ggacgcggtc agcgaggggg
       61 tgtacttcgt cgatctcgag cgccggatca cctactggaa cagcggggcc gagcggatca
      121 cgggcttccg cgccgaggag gtgatcggca gccgctgctc cgacaacatc ctgcgccatg
      181 tcgacgaggc ggggaaggag ctctgcgcgg gcgactgtcc actgagcatc gccatggcga
      241 gcggggccaa gcagcaggcc aacgtcttcc tccaccacag ggacggccac cgcgtcccgg
      301 tcgcggtcag gggcatcccc tacttcgacg agaaggaggc cgcggtcggg gcaatcgagg
      361 tcttcacgga ccgctcggag cgcctcgcca tcctcgccga gctccaggag ctcaggaagg
      421 agaacctcag cgacagcctc accgggctcg ggaaccgccg ctatttcgac atacgcctcg
      481 agcccctgct cgcggccctc cgcggcggtg gcggcccctt cggcctcctc ctcatcgaca
      541 tcgaccactt caagcagatg aacgacgact acggccacgc gaccggggac cgcatcctcc
      601 gcatggtggc ctggaccctc gccaacgcgg tgaggcgggg cgacgtctcc gtgcgctggg
      661 gcggggacga gttcgtcgtc ctcgcgccca aggccacgcc gatggtcctg ggcgccctgg
      721 ccgagcggat cagggccctc gtcgcgagcg gctggctcag cctcgacggg ggccgcagcg
      781 tctcggtcac ggtctcggtc ggcggcgcga tctcggccgc gggcgatgac tccgacgccc
      841 tcgtgcggcg cgctgacctg ctgctctacc gcagcaagga ggggggcagg aacagggcga
      901 cgatcgaggg ggagccgatg ggggcgacga caggggcggc gacgacgggg gcggcggaag
      961 aggcagtccc cgaatcggga ggaggcggaa tgctggaact tggagccgtg caggagaccc
     1021 tgatgatgcc cctctggggc cgggcccatg agacgaggaa ggcccttccc ctcctcaggg
     1081 atgaggctgc ggcggcgatc gtggagggga tcgactacga ctttacgcgc atcgcgaagc
     1141 gggtcaaccc gctgagccgc gccggctgga tagcgagggc catctacttc gacgaggcca
     1201 tccgttcctt cctgcgcgag cacgagaggg cggcggtcgt caacctcggc tgcggcctcg
     1261 acacgagctt cgaccgcgtc gacgacggga aggcgcactg gtacgagctc gacctccccg
     1321 acgtgatcga cctcagaagg cgcttcatcg ccgaagtcga gcggcgggtc ttcatcgctg
     1381 actcggccct cggctccgcc tggtacgaca ggatcgagaa aggctcctcg gtcctcatcc
     1441 tcatcgcggg ggtcatctac tatttcgagg aggccgagct ccgctgtctc ttccgcgaaa
     1501 tcaggcgccg cctcggcagg gccgagctca tcttcgacta cgcatcgaag cggggcatgc
     1561 gcgccgccaa ccgcagcgtg atcggcaagg cggggatggg cgacgaggcc cggctgaaat
     1621 ggggcatcga cgagctccgc gagctcgagg cctgggactg cggtctcagg atcctcgacg
     1681 acaggcccat cttccgcgac cacaggaaac gctacccgcc cagccgccgc ctcggcatgg
     1741 ccctttcgga cgcgctcggg atcatgtcgc tcgcgaggat cgaaatcgcc tagccacggc
     1801 gccccgccta gccgaccctg accctgatgc cgtgctgctt gaggagggca gccgtgaccc
     1861 cgtcgccctc gattaggcgg tgggagaagc tgccatcgaa gatccagccg cagccgcagg
     1921 aggggctctt ctcgatgagg atggcctcct cggccccgag ggagcgcgct atggcgagga
     1981 cggcgtaggc tcccttcagg aactcgctcg tgaggtcctc gccatccgag tccagcacct
     2041 tcgcctcgcc gcggaggacc tcggccccgc cgcctccgac gatctcggcg gcatggcggg
     2101 ggcagccgag gccacccagg atctcggggc agaccgggaa gagcccgccc tgggcgaact
     2161 ctgcgacgag gccggcctcg gccttcccct cggccctgta gttgcacttg atcccgagga
     2221 ggcaggcgct cacgagcttc atggcttctc ccgtccgacg ctatcccccg cgcctagggc
     2281 cgactatagc cccgccccac caagccggca aggcttcgct cctggacgag tcgcgggccc
     2341 cgctggtcct cccctcctcg agctcgagga tcccgtcgca gagccgctcg agctcgctgc
     2401 gcgagtggct cacgaggatg gccgtgaccc cggtccgcct ctggatgcgc cgtagctcct
     2461 ctccgaggct gtggcggagg tcctcgtcga ggg
//
LOCUS       DASSQV010000204         5970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_424402, whole genome shotgun sequence.
ACCESSION   DASSQV010000204 DASSQV010000000
VERSION     DASSQV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424402"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VFL04_03530"
     CDS             <1..1494
                     /locus_tag="VFL04_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7838806.1"
                     /translation="VGNAIKFTPAGRVELRVELKQREEGRARIGFAVHDTGIGIAPDQ
                     KERLYQPFTQADSSTTRRFGGTGLGLSICRELVNLMGGELEMASEPGKGSVFSFAIWL
                     DLAEGVEEGPPHPGGQGCAVPVPKMPVPDFRGLRALIAEDSDINVEIATEFLRETGIS
                     VEVAHNGTDAVAKVLRSDPAFDIILMDIQMPGMDGYEAARLIRSDPRFAAVPIVAMTA
                     HAFAEERRRSLQVGMNEHLAKPIDGELLLAAMARLLAPARPESIPPDGTMATKEVFIA
                     AQDCALDFADGLARASGNERLYGELLRRFASQYEDAAAILEELALKEDADSAAKFAHK
                     MRSVAGNLGLSELNRRAGELESALASGRPDGLSWAVKRFSAALPRTMSAIAEALARLE
                     PEPHQGEAANGRPGSSLETGREDGAAPGMEPAHAREVSDLMRRYLEIGSSEAIALAAR
                     ERRAFASILGPELLLRFDDSLAVLDYESALGYLDGQNSRWGHHEDNA"
     gene            1478..2635
                     /locus_tag="VFL04_03535"
     CDS             1478..2635
                     /locus_tag="VFL04_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7838807.1"
                     /translation="MKTMHDTKGESGGRPRILIVDDSPENILLLIEILKTEYEIIVAT
                     SGEQALKRLEASPCLDLILLDVVLPGLNGFEVCAMLKTKRATRSIPVIFLSALDTEAN
                     ESRGLEVGGVDYITKPYSPSIVRARIRSHLELKRHRNRLEDLVLERTEELTLTQAVTF
                     MSLGTLAEFRDPETGGHIQRTANYVRLLCQRLSRSDDFCAVLTPPMIERLAQSAPLHD
                     IGKVGIPDTILLKEGPLSAKEFEIMKTHAELGYRALMQSTLMLGGNSFLNIAMDVALS
                     HHERWNGEGYPRRFAEEETPLAGRIMKLADVYDALTTRRPYKPPFSHENSKAIMCEEM
                     NEHFDPRILRAFLELEDEFKAVASKYQDAEEPAGCEDTRRFREQTDRVLPC"
     gene            2746..4068
                     /locus_tag="VFL04_03540"
     CDS             2746..4068
                     /locus_tag="VFL04_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7838808.1"
                     /translation="MILTEEMQAASALLKDEGERLIWISGRAGTGKSTYLQYLKTVLR
                     PRNAVYLAPTGVAALSIGGQTIHSFFWIDPREEVYLETKLDAAREAQLFPLLAKVETL
                     VIDEISMVRADLLDEMDRRMRRAKGEPRQPFGGARLVLFGDPFQLPPVEPEEASRAAA
                     LFDERYKSPFFFSSKVLRTRALAMKLVEFTRVFRQSEADFLATLDHCRSGRVTGADLA
                     LLASRVLPEGKKPPVDHLALTATRSEAQRLNRYRLDALPGPSQRFSASAAGQFTKTLD
                     EEELPVPRELELKTGARVLLTVNDEGGRWANGALATVVGLDETGVTLSDEMGEFRIEA
                     YGWRRVKFVLRDGQIVEEVTGQYTQLPLQLGWALTIHRAQGRTLERVFVDLSQGAFAA
                     GQAYVAISRVTKLEGLVLRTLPRARDFFVHERVGVFLDYVKKRGGVRG"
     gene            complement(4112..4873)
                     /locus_tag="VFL04_03545"
     CDS             complement(4112..4873)
                     /locus_tag="VFL04_03545"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET7838809.1"
                     /translation="MSTKHEPGRRIAAALAVALLACLLLSCAPGSLPLAQKGTPSTWS
                     YQTLSSPAGGRGIAAARSLPPASSSWPVLAGLKATGKSPLMSKLPAVTVADATSAASS
                     VATPPVPHPLADHKDCLACHAVGTGSKPAPENHKERGIDVCLYCHSPQEGASAIPPLP
                     AKPSAAFCLGCHGPFEDVAKKSADFVTEDGEKVNPHVFVPHNSTKIVNCIECHEPHEL
                     PATAATVLSKPELQYCFSCHHQQDFRPCSECHKEG"
     gene            complement(4991..>5970)
                     /locus_tag="VFL04_03550"
     CDS             complement(4991..>5970)
                     /locus_tag="VFL04_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7838810.1"
                     /translation="GTGGSTGNVNFRRMFDSRLTEEYPLAAAEYSPQDASGELAAMAI
                     GASLWGTANQTLERNGSLRKRPIVGTRTNYIEWTPKSPLFPKVKAQGLNVRNWQDAII
                     VNQVGKRFYNEMEDGYPNGTVQGSLNPYKPADWRNAQKTKYTPMNYTDAALAINEGST
                     APDFASGPQWAIFDAAAVKREKWNLTPTSTHPDYFFSAPTLTELAAQISKCPYQKVKM
                     PAEFLEATVKRYNQMVDLGVDPDFAKPSPQYKIETGPFYAAWATFTVHDTYAGLRINM
                     KCQVMDMKGEVIPGLYCGGESAAGSSQHGLGRCTTQGYIAGGAAAAEIV"
BASE COUNT         1040 a         1917 c         1972 g         1041 t
ORIGIN      
        1 gtcggcaacg ccatcaaatt cacgccggcc ggaagggtag agctgcgggt ggagctcaag
       61 cagcgggagg aagggcgggc caggattgga ttcgccgtgc acgacacggg cataggcatc
      121 gctcccgacc agaaggagcg gctttaccag cccttcactc aggcagacag ttcgacgaca
      181 aggaggtttg gaggaacggg gctgggcctg agcatctgca gggagctcgt gaacctcatg
      241 ggcggtgagc tggagatggc cagcgagcca ggcaagggca gcgtgttcag tttcgccatc
      301 tggctcgacc tggccgaggg cgtggaggag ggcccgccgc acccaggcgg gcagggctgc
      361 gccgtgccgg tgcccaagat gcccgtcccc gacttccgcg gcctcagggc cctcatcgcc
      421 gaggacagcg acatcaacgt ggagatagcg accgagttcc tgcgtgagac cggcatatcc
      481 gtcgaggttg cgcacaatgg caccgacgcc gtcgcgaagg tccttcgctc cgatccggcc
      541 ttcgacatca tcctcatgga catccagatg cccggtatgg acggctatga ggccgcccgc
      601 ctcatccgct ccgacccccg cttcgccgcg gttcccatcg tggccatgac cgcccacgcc
      661 ttcgccgagg agaggcggcg gtccctccag gtggggatga acgagcacct ggccaaaccc
      721 atcgatgggg agctcctctt agcggcgatg gccaggctcc tcgccccagc acgcccggag
      781 tctatccccc ccgatgggac catggccaca aaggaggtct tcatcgccgc gcaggactgc
      841 gccctcgact tcgccgatgg cctcgcccgg gcttcaggca acgagaggct ctatggcgag
      901 ctgcttcggc gcttcgcctc gcaatacgag gatgcggccg cgatcctcga ggagcttgca
      961 ttgaaggagg acgcggattc agcggcgaaa ttcgcgcaca agatgaggag cgtcgcgggc
     1021 aacctgggcc tcagtgagct gaacagaagg gccggggagc tggagagcgc ccttgcctcg
     1081 ggccggccag acgggctgtc ctgggcagtg aaacggttct cggccgcttt gccccggacg
     1141 atgtcggcca tcgcggaggc cctagcccga ctcgagcctg agccccacca gggagaggcg
     1201 gcaaatggcc gccccggatc gagcttggag acgggccgcg aggatggcgc agcaccgggg
     1261 atggagccgg cccatgcccg tgaggtctcc gatctgatgc ggcgctacct cgagatcgga
     1321 tcgagcgagg cgatcgccct cgcggctcgg gagagaaggg ccttcgcctc gatcctcgga
     1381 cccgaattgc tcctccgttt cgacgactcc ctcgccgtcc tcgattacga gtcggcgctg
     1441 ggctatcttg acggccagaa cagccgatgg ggacaccatg aagacaatgc atgacaccaa
     1501 gggcgaaagc ggcggcagac cccggatcct catcgtcgat gattcccccg agaacatcct
     1561 cctcctcatc gagatcctca agaccgagta cgagatcata gtggccacga gtggtgagca
     1621 agccctgaaa cggctcgagg cctcgccctg tctcgacctc atcctcctcg atgtcgtgtt
     1681 gcccggattg aacggcttcg aagtatgcgc gatgctcaag acgaagcggg ccacccgctc
     1741 gatccccgtg atcttcctct cggccctcga caccgaggca aacgaatccc gcggcctcga
     1801 ggtcggtggc gtcgactaca tcacgaaacc ctacagcccg tcgatcgtca gggcccggat
     1861 caggagccac ctcgagctca agaggcaccg caaccgcctc gaggacctcg tccttgaacg
     1921 gaccgaggag ctgaccctga cccaggctgt cacgttcatg agcctcggaa cccttgctga
     1981 gttccgggac cccgagaccg ggggccacat ccagcggacg gccaactacg tccgactcct
     2041 ctgccagcgc ctttcgcgct ccgacgactt ctgcgccgtc ctcaccccgc ccatgataga
     2101 gcggctcgcc caatcggcgc ccctgcacga cattggcaaa gtggggatcc ccgacaccat
     2161 cctcctcaag gaaggcccgc tcagcgccaa ggaattcgag atcatgaaga cccacgccga
     2221 gcttggctac cgcgccctca tgcagtcgac gctgatgctc gggggcaact ccttcctcaa
     2281 catcgcgatg gacgtagcgc tctcccacca cgagcgctgg aacggggagg gctatccccg
     2341 ccgtttcgct gaggaggaga cccccctggc gggaaggatc atgaagctgg ccgatgtcta
     2401 cgacgccctc acgacgaggc ggccctacaa gcctcccttc tcccacgaga actccaaggc
     2461 gatcatgtgc gaggagatga acgagcattt cgatccgagg atcctccggg ccttcctcga
     2521 gctcgaggac gagttcaagg ccgtcgccag caaataccag gacgccgaag agccggcggg
     2581 ctgcgaggac acaaggcgct tcagggagca gacggacagg gtcctgccct gctgagccac
     2641 cggatcccct ctcccagccc aatagcctcg cgttttccca ttgccaagct cggtcttttc
     2701 cggtcttatt tccatgatga cgggggcagc ttggcggtgg acacaatgat cctgacggag
     2761 gagatgcagg cggcgagcgc cctgctcaag gatgagggcg agcgcctgat ctggatctcg
     2821 gggcgggcgg gcacgggcaa gtccacctac ctccagtacc tgaagaccgt gcttcgtccg
     2881 aggaatgccg tctacctcgc gccgacaggg gtagccgccc tctccatcgg tgggcagacg
     2941 atccacagtt tcttctggat cgacccaagg gaagaggtgt accttgagac caagcttgac
     3001 gccgcaaggg aggcccagct cttccccctc cttgcgaagg ttgagaccct tgtcatcgat
     3061 gagatatcga tggtccgggc cgacctcctt gacgagatgg atcgaaggat gcgtcgggcg
     3121 aagggcgagc ctcgccagcc cttcggcgga gcccgtctgg tcctcttcgg cgatcccttc
     3181 cagcttccgc ccgtcgagcc cgaagaggcg agccgcgcag ccgccctttt cgacgagcgc
     3241 tacaagagtc cctttttctt ctcatcgaag gttcttcgca caagggccct ggccatgaaa
     3301 ttggtggagt tcacgagggt attccggcag agcgaggcgg atttcctggc caccctcgac
     3361 cattgccgct ccggccgggt gacgggcgcc gatcttgccc ttctcgcctc cagggttttg
     3421 cccgagggga aaaagccccc cgtcgaccac ctcgccctca cggccacgag aagcgaggcc
     3481 caaaggctga accggtatcg cctcgatgcc ctgcccggtc cttcgcagcg cttctccgcc
     3541 agcgccgcag gccagttcac caagaccctc gacgaggagg aactccccgt tcccagggag
     3601 ctcgagctca agacgggcgc gagggtcctc ctcaccgtga acgacgaggg cgggcgctgg
     3661 gccaacggcg ccctcgccac cgtggtcggc ctcgatgaga cgggggtcac cctcagcgat
     3721 gagatgggcg agttccggat tgaggcctat ggctggaggc gggtcaagtt tgtcctgcgg
     3781 gatggccaga tcgtggagga ggtcaccggc cagtacacgc agctgcccct ccagctcggt
     3841 tgggccctca cgatccaccg tgcccagggc cgcaccctgg agagggtctt cgtcgacctc
     3901 tctcaaggag ccttcgcggc cggtcaggca tatgtcgcca tctcgcgcgt cacgaagctg
     3961 gagggcctgg tgctccgcac ccttccccgc gcacgcgact tctttgtcca tgagcgggtc
     4021 ggggtcttcc tggactacgt gaagaagcga ggcggagtcc gcggataggg gctcatcatc
     4081 gtccccgcat cgatggcgcg atggcgggcg atcagccctc cttgtggcac tcggagcagg
     4141 gcctgaagtc ctgctggtgg tggcaggaga aacagtactg caattcgggt ttcgagagca
     4201 ccgtggctgc cgtggccggc agctcatggg gttcgtggca ctcgatgcag ttgacgatct
     4261 tagtcgagtt gtgcggaacg aagacatgag gattgacctt ctctccatcc tcggtcacga
     4321 agtcggccga cttcttggcc acatcctcga atggcccgtg acagcccagg caaaaggccg
     4381 cgctcggttt ggcaggcaaa ggggggatgg ctgaggcgcc ctcctgaggg ctgtgacagt
     4441 agagacagac gtcgatcccc ctctccttgt ggttctccgg cgccggcttt gacccggtcc
     4501 cgaccgcgtg gcaggcgagg cagtccttgt ggtcggcaag ggggtgtggc accggcggcg
     4561 tcgccactga tgatgctgca gaggtggcgt cggctacagt gaccgcggga agctttgaca
     4621 tgaggggcga tttgcctgtg gccttcaggc cggccaagac gggccacgag ctgcttgcgg
     4681 ggggcaggct cctggcggcg gcgatgcctc tgccgccggc cgggcttgag agggtctggt
     4741 agctccaggt cgaaggagtc cccttctggg ccaggggcag ggaacccggc gcgcaggaga
     4801 ggagaaggca agcgaggagg gccacggcga gggccgccgc gatcctccgg ccgggttcat
     4861 gtttggtgct cattgattcc tcgtttgttc tgatcctagg ttcccaccag ggcggaagca
     4921 aggggcggcc ccggagccgc gggacggcaa gcgccgtccc cgaccctcgg gccgccccaa
     4981 aagctgtgtg ctagacgatc tcggcggcgg ctgctccgcc ggcgatgtat ccctgggtcg
     5041 tacagcggcc gaggccgtgc tggctcgagc ccgcggcgga ttcgccgccg cagtacaggc
     5101 cggggatgac ctcgcccttc atgtccatga cctggcattt catgttgatg cgcagcccgg
     5161 cgtatgtgtc atgcacggtg aaagtggccc aggccgcgta gaaggggccc gtctcgatct
     5221 tgtactgggg gctgggcttg gcgaaatcgg ggtcgacgcc gagatcgacc atctggttgt
     5281 agcgcttcac cgtggcctcg aggaactcgg cgggcatctt caccttctga taggggcact
     5341 tggagatctg tgccgccagc tcggtgaggg tcggggcgct gaagaagtag tcggggtggg
     5401 tcgaggtggg agtgaggttc cacttctccc gcttcacggc cgcggcatcg aatatcgccc
     5461 actggggtcc cgatgcaaag tcgggagctg tcgagccctc attgatggca agcgcggcat
     5521 cggtgtagtt catcggggtg tatttggtct tctgcgcgtt gcgccaatcg gccggcttgt
     5581 aggggttgag ggatccctgc acggtgccgt tcgggtagcc gtcctccatc tcgttgtaga
     5641 agcgcttgcc cacctggttg acgatgatcg catcctgcca attcctgacg ttgaggccct
     5701 gagccttgac cttggggaag agcggcgatt tgggtgtcca ctcgatgtag ttcgtgcggg
     5761 tgccgacgat aggccgcttg cggagcgagc cgttgcgctc gagggtctgg ttggccgtgc
     5821 cccagagcga ggcgccaatc gccatggccg cgagctcgcc cgatgcgtcc tggggggaat
     5881 actccgcagc cgcgagcggg tactcttcgg tgaggcggga gtcgaacatc cggcggaagt
     5941 tcacgttgcc agtgctgccg ccagtgccca
//
LOCUS       DASSQV010000205         4024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1295048, whole genome shotgun sequence.
ACCESSION   DASSQV010000205 DASSQV010000000
VERSION     DASSQV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295048"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..404)
                     /locus_tag="VFL04_03555"
     CDS             complement(90..404)
                     /locus_tag="VFL04_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7838811.1"
                     /translation="MPATPAVSKPLTKAQIVTQLAEKNGLTKKQAAAFIESLVDLACK
                     ETKKNKKFTLPGIGILKLNKRKARVGRNPATGESIKIPAKTTVKMTVSKAAKDAILGP
                     SK"
     gene            complement(498..1250)
                     /locus_tag="VFL04_03560"
     CDS             complement(498..1250)
                     /locus_tag="VFL04_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET7838812.1"
                     /translation="MNAIILAAGMGTRLRPLTDDRPKSLVEVAGESFFARQLRQLRSA
                     GVSEVSVVTGYMAEAFAPWRGQSGLSFVHNDHYDDRNNLWSMYLVRDRLADTLVLDGD
                     VWLAEGVIPAESPPASCWFVGPRQEMRNEWVVRADDRGRVQRIDVASGSGWILSGLSY
                     WTAADGRLLARRVEEAALAPGNEGIYWDEMPRRALGEIAVMARRIGADDWTEIDTLED
                     KAALEARLDKIAQRVAPDGPISARQPRARADD"
     gene            complement(1247..1927)
                     /locus_tag="VFL04_03565"
     CDS             complement(1247..1927)
                     /locus_tag="VFL04_03565"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET7838813.1"
                     /translation="MSQILAFFGPIVADHTYLAFFVFLLVFGFTLPISEEIALALVGV
                     AARSQDLILPECLVPALPALLLADLLYYTIARLIGPRLLRMKLFIRFMKPERVCEGER
                     YFQRRGQRIVFFCRFVIGLRAPAILSAGFLRMPLKRFIAFDGLALLIATPIWLAFGYA
                     LGAQLDSQVGTLGKIIAFVGPLAIVAGTVLVYRSVKADRARAEAEGFETLDQAAEAES
                     GCEKRVGP"
     gene            complement(1924..2994)
                     /locus_tag="VFL04_03570"
     CDS             complement(1924..2994)
                     /locus_tag="VFL04_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7838814.1"
                     /translation="MSISEHKGSGARKLGPAVVASGDAVAPAGKGAPKPDTNILEGLR
                     RFIDGRRIEAALPPGLRRRGVVLEGLQPMGGLTNRNYKVFVGGEALVLRLPGRGTGRF
                     IDRSTERANHEAAARAGFTPQSIFFDGRTGVKITRYLHQSRALDPASSRDRETSADVA
                     RLLSSFHRSGLRFVNDFDVFRLIRTYERVARGRFARFYDGYAGVRDRVLALERPLSVL
                     APERVACHNDLVPENILVTPEGLTLIDWEYSGMNDPAWDLASFTLESGYGEEDETRFL
                     AAYYGGPVPERLRIRVETYRLLQDFLWSLWSILQETANRDPRKARSYREYGQDRYARA
                     RSRLKPVESRFGISRHLEEPRE"
     gene            complement(2991..3713)
                     /locus_tag="VFL04_03575"
     CDS             complement(2991..3713)
                     /locus_tag="VFL04_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838815.1"
                     /translation="MSPNEELLADATALVASASEGGLILRLLGGIAVYASCPSSRLPP
                     FARSCGDIDLVSPEPAGRITPLFEALRWVPDAEFNLYNGDSRLCFHKGGKKADVFLGG
                     FRMCHEIPLGGRYGEGPLTLPLAELLLTKLQVFEANSKDLDDASCLLLDHPVRLEDGE
                     AINAAAIAHACSADWGLWRTVSKNLGLLRGRLGEASAEAQERIAHIEETVSAEPKSLG
                     WRMRSLVGDRMRWYELPEEVER"
     gene            complement(3719..>4024)
                     /locus_tag="VFL04_03580"
     CDS             complement(3719..>4024)
                     /locus_tag="VFL04_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838816.1"
                     /translation="VILPFVKKDLYAASPLAKFRLGKVPLITVFGLVFCAFLGYLLYE
                     WVIDPNGLYGISYRNVTSVVFMGALYLLAAGIYVGVRGYRRRSGIDVNKIYGEIPVE"
BASE COUNT          652 a         1266 c         1437 g          669 t
ORIGIN      
        1 gccacgggtg cgaagacggg acagcctccg ctcgggcgtc ttgggtcgcc cgtgtagcgg
       61 aggctgtctg gggaggggaa gttaccgggc tatttggacg ggccgaggat ggcgtcctta
      121 gcggccttgg agaccgtcat cttgaccgtg gtcttcgcgg ggatcttgat cgactcgccg
      181 gtggcggggt tgcgcccgac cctggccttg cgcttgttga gcttgaggat cccgatgcca
      241 gggagggtga acttcttgtt cttcttcgtt tccttgcagg ccaagtcgac gagggactcg
      301 atgaacgctg cagcctgctt cttcgtgagg ccgttcttct cggcgagctg ggtgacgatc
      361 tgggccttcg tcaggggttt cgagacggcg ggcgttgctg gcattggcat ctccttatag
      421 agtttcaaac atcataaacc cgtgggccgc cctccgcaag tgaattttca cccgggcggg
      481 catccccgga tcctgagcta gtcatccgcg cgtgcgcgcg gttggcgggc cgaaatgggg
      541 ccatcgggag cgacgcgttg tgcgattttg tccagtctcg cctcgagggc agccttgtcc
      601 tccagggtgt cgatctcggt ccagtcatcc gcgccgatcc gccgggccat gacggcgatc
      661 tcgccgaggg ccctcctggg catctcgtcc cagtagatgc cctcgttgcc cggagccagg
      721 gcggcttcct ctacacgccg ggcgagcagc ctgccgtcgg ccgccgtcca gtaggagagg
      781 cccgacagta tccagcccga gccgctcgcg acgtcgatcc tctgcacccg cccccggtcg
      841 tcggcgcgca ccacccactc gttgcgcatc tcctgcctcg ggccgacgaa ccagcagctc
      901 gcgggcggac tctcggcggg gatgacaccc tcggccagcc atacgtcgcc gtcgaggact
      961 agggtgtcgg cgagccggtc ccggacgagg tacatggacc agaggttgtt ccggtcatcg
     1021 taatggtcgt tgtgcacgaa gctgagcccg ctctggcccc gccagggcgc gaaggcctcg
     1081 gccatgtagc ccgtgacgac ggagacctcg gagacgcccg ccgaacgcag ctggcgcagc
     1141 tggcgggcga agaagctctc gccggcgacc tcgacgaggg acttcggcct gtcgtcggtc
     1201 agaggcctga gcctggtgcc catccccgcg gcgaggatga tcgcgttcat ggcccgaccc
     1261 tcttctcgca gcccgattcc gcctcggcgg cttggtcaag ggtctcgaag ccctctgcct
     1321 cggcccttgc cctgtcggcc ttgacgctcc tgtagaccag gacggtcccg gcgacgatgg
     1381 ccaggggccc gacgaaggcg atgatcttgc cgagggtccc cacctggctg tcgagctggg
     1441 ctccgagggc gtagccgaag gccagccaga tgggcgtggc gatgagcagg gcgaggccgt
     1501 cgaaggctat gaagcgcttg agcggcatgc ggaggaagcc ggcgctcagg atcgccgggg
     1561 cccgaaggcc gatgacgaag cggcagaaga agacgatcct ctgcccccgc ctctggaaat
     1621 accgctcccc ctcgcagacc cgctcgggtt tcatgaagcg gatgaagagc ttcatccgga
     1681 ggagccgcgg cccgatgagc cgggcgatcg tgtaatacag gaggtcggcg aggaggagtg
     1741 ccggaagggc cgggacgagg cattccggca ggatgaggtc ctggctccga gccgcgaccc
     1801 cgacgagggc gagggcgatc tcctccgaga tgggcagggt gaagccgaag accaggagga
     1861 acacgaagaa ggcgaggtag gtatggtccg cgacgatggg tccgaagaag gcgaggatct
     1921 ggctcattcg cggggctcct ctaggtgtcg ggagatgccg aagcgtgact cgactggttt
     1981 gagcctcgac cgggcgcggg cgtagcggtc ctggccgtac tccctgtagc tcctggcctt
     2041 gcggggatcg cggttggccg tctcctggag gatcgaccag agcgaccaga ggaagtcctg
     2101 gaggagcctg taggtctcga ccctgatcct gagccgctcg gggacggggc cgccatagta
     2161 ggccgcgagg aagcgagtct cgtcctcttc gccatagccg gactcgaggg tgaaggaggc
     2221 gagatcccag gccgggtcgt tcatccccga gtattcccag tcgatgaggg tgaggccctc
     2281 gggggtgacg aggatgttct ccggaacgag gtcgttgtgg caggcgacgc gctcgggggc
     2341 gaggacggac aggggcctct cgagggcgag gacgcggtcg cggaccccgg cgtagccgtc
     2401 gtagaagcgc gcgaaacggc cccgggcgac ccgctcgtag gtgcggatga ggcggaagac
     2461 gtcgaaatcg ttgacgaaac gcagcccgga cctgtggaag gacgagagga gcctggccac
     2521 gtcggccgag gtctccctgt cccggctcga ggcgggatcg agggcgcggg actggtgcag
     2581 gtagcgtgtg atcttcaccc cggtgcgtcc atcgaagaag atggactggg gcgtgaagcc
     2641 ggccctcgcc gccgcctcgt ggttggcccg ctcggtcgag cggtcgatga accttcccgt
     2701 gccgcggccg ggaagcctga gcacgagggc ctcgccgccg acgaagacct tgtagttgcg
     2761 gttggtcagg cctcccatgg gctgaagccc ctcaagcacc acgcccctgc ggcgcagccc
     2821 gggggggagg gcggcctcta tcctcctgcc gtcgatgaag cgtcggaggc cctcgaggat
     2881 gttggtgtcg ggtttcggag cgcccttgcc cgcgggcgcg accgcgtcgc cggaggcgac
     2941 cacggcgggg ccgagcttcc tcgcgcctga ccccttgtgt tcactgatgc tcaacgctcg
     3001 acctcttccg gaagctcgta ccagcgcatc ctgtcgccca ccagggagcg catgcgccag
     3061 cccagggatt tcggctcggc cgagaccgtc tcctcgatgt gggcgatccg ctcctgggcc
     3121 tcggcgctcg cctcaccgag gcgcccgcgc aggaggccca ggttcttgct gaccgtcctc
     3181 cagagtcccc agtcggccga gcaggcgtgg gctatcgcgg ccgcattgat cgcctcccca
     3241 tcctcgagac ggacgggatg gtcgaggagg aggcaggatg cgtcgtccag atccttggag
     3301 ttggcctcga agacctggag ctttgtgagg aggagctcgg cgaggggcag ggtgaggggg
     3361 ccctcgccgt atctccctcc caaggggatc tcgtggcaca tgcggaaccc gcccaggaag
     3421 acgtcggcct tcttgccccc tttgtggaag caaagccttg aatccccatt atacagattg
     3481 aattcggcgt cgggaaccca gcgcagggcc tcgaacaggg gagtgatccg gccagccggc
     3541 tcagggctca cgagatcgat gtccccgcag ctgcgcgcga agggggggag gcggctcgac
     3601 ggacagctgg cgtagacagc gatgccgccg agcagcctca ggatgaggcc cccctcggat
     3661 gccgaggcga caagggccgt cgcgtcggca agcagttcct cgttcgggct catcgcgctc
     3721 actcgaccgg gatttccccg tagatcttgt tgacgtcgat gcccgagcgc ctccggtagc
     3781 ccctcacgcc gacgtagatg ccggcggcga gcaggtagag ggcgcccatg aagacgacgg
     3841 acgtgacatt tcgatagctg atcccgtaga gcccgttcgg gtcgatcacc cactcgtaga
     3901 ggaggtagcc gaggaaggcg cagaagacca ggccgaagac cgtgatgagc gggaccttcc
     3961 cgagccggaa cttcgcgagc ggcgaggccg cgtagaggtc cttcttgacg aagggcagga
     4021 tcac
//
LOCUS       DASSQV010000206         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1295114, whole genome shotgun sequence.
ACCESSION   DASSQV010000206 DASSQV010000000
VERSION     DASSQV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295114"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VFL04_03585"
     CDS             complement(<1..887)
                     /locus_tag="VFL04_03585"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7838817.1"
                     /translation="MKRSVASVLILVIGLAFAASPLFAQGAWKPNKTITLIIPWGAGG
                     ASDLTARVVAGEMEKVLGAKVAVINQPGASGSTGTQSAWNDPHDGYTWTGNADASVAT
                     YQVDGLTPKISHKDWQAYFAMKNPAIICVNPASPIKTWDDLVKAFKTREVSVATAGVG
                     AGGHIAAETVKKALGISYKHVPYGGGNPAVIATVSGECEVIMQLSMEEADMIKAGKLR
                     AIAVMTPAPLQLQGMGAIPSINDFVKDMPSVAFNFGLFIPKDIPANAKAAISTAFDVA
                     AKSKAVKDLADSKACVATA"
     gene            complement(979..1794)
                     /locus_tag="VFL04_03590"
     CDS             complement(979..1794)
                     /locus_tag="VFL04_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET7838818.1"
                     /translation="MRYAICNELFGAMDFAASCGMIAAAGFTGIEIAPFTLGPDPSAL
                     GPDRVSALRRTILDSGLSFAGMHWLLAAPPGLHLASAEAATRRRTVDFLKAMCDLAGG
                     LGGGMLVLGSPRQRGAQHVPASEALAFLREALAECGPHAAACGSKILLEALSSAQTEV
                     VNLMSEAEAMVRGIGGPGLGCMLDFHNCVDEVEGFDALVDRHHALIRHVHLNDPDGGH
                     PRAGDLSYLPAFRRLVAHGYEGWVSLEIFGVPEDPGLVLSETMDYLRGIEAEL"
     gene            complement(1791..>2874)
                     /locus_tag="VFL04_03595"
     CDS             complement(1791..>2874)
                     /locus_tag="VFL04_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET7838819.1"
                     /translation="LCGTTVTGIATGILAKVLGAKGLPTETRVADTVQGISAPPPELL
                     LALGSDTLRVGMDRIRGGLKTGDDGSLETVDSFGVLWRRAQGQLYYSQASAPLFEGRL
                     SDALEAYRFPEPELGPMRAAVERGRLDSDRLGLFPILDRDCAGLLEMSARLRGTERLY
                     LDMYDDPEGVEELAERLLEYKLAYWQAQVSFWGAATAGIAEADDYGSEISLLISPHMV
                     RRTYIGRYARLFSAIRSRLPDARIVFHCCGAIREIIGDLIEAGVDALNPVQYAAAGME
                     PAALKRDFGDRLSFWGGAVDTKRVLPLGSPAEVRAELLRNAAALGPGGGWVISAVHNI
                     QEDVPLENALAFLDAVAELKAKGEGR"
BASE COUNT          455 a          954 c         1014 g          451 t
ORIGIN      
        1 atcgccgtgg cgacgcaggc cttggagtcg gcgaggtcct tgacggcctt gctcttggcc
       61 gcgacgtcga aggccgtcga gatggcggcc ttggcgttgg ccgggatgtc cttcgggatg
      121 aagaggccga aattgaaggc cacgctcggc atgtccttca cgaagtcgtt gatcgacggg
      181 atcgccccca tgccctggag ctggagtggg gccggggtca tgacggcgat ggccctcagc
      241 ttcccggcct tgatcatgtc ggcctcctcc atggagagct gcatgatcac ctcgcactcg
      301 cccgagacgg tcgcgatgac ggccgggttg ccgccgccgt atggcacgtg cttgtagctg
      361 atgccgaggg ccttcttcac cgtctccgcg gcgatgtggc cgccagcccc gacgcccgcg
      421 gtcgcgaccg agacctcgcg ggtcttgaag gccttcacca ggtcgtccca ggtcttgatg
      481 ggggaggccg ggttgacgca gatgatggcg gggttcttca tcgcgaaata ggcctgccag
      541 tccttgtggg agatcttggg cgtgaggcca tcgacctggt aggtcgcgac gctcgcgtcg
      601 gcgttgccgg tccaggtgta gccgtcgtgg gggtcattcc aggcggattg ggtgcccgtc
      661 gagcccgagg cgcccggctg gttgatgacg gcgaccttgg ccccgaggac cttctccatc
      721 tcgccggcga ccacgcgggc cgtgaggtca gaggcccccc ctgcgcccca ggggatgatc
      781 agggtaatgg tcttgttggg cttccaggcg ccctgcgcga agagggggct cgccgcgaag
      841 gcgaggccga tgaccaggat gagaacggat gcgacagatc tcttcatgga tgcatgtctc
      901 cttttccccg caatcctgcc ccccgacggg ggaggtgacg aaacaaggca aggcgcatca
      961 ccgcgccagt gaccaccatc acagctctgc ctctatgccc cgcaggtaat ccatggtctc
     1021 ggagagcacg agcccgggat cctcggggac accgaaaatc tcgagcgaga cccatccctc
     1081 gtacccgtgg gccacaagcc gcctgaaggc cggaaggtag gacaggtcac ccgccctcgg
     1141 atggccgcca tcgggatcgt tgaggtggac gtggcggatc agggcgtggt ggcggtccac
     1201 gagggcatca aagccctcta cctcgtcgac gcagttgtgg aagtcgagca tgcagccgag
     1261 cccggggccc ccgatgcccc tcaccatggc ctcggcctca gacatgaggt tgaccacttc
     1321 ggtctgggcc gaggagaggg cctcgaggag gatcttggat ccgcaggccg ctgcgtgggg
     1381 cccgcactcg gccagggcct cgcggaggaa ggccaaggcc tcggaggccg gcacatgctg
     1441 cgcgccgcgc tgcctcggcg agccgaggac gagcatgccc ccgccgagac caccggcgag
     1501 atcgcacatc gccttcagga agtcgacagt gcgcctcctc gtcgcggcct cggccgaggc
     1561 caggtggagg cccggggggg cggcgaggag ccagtgcatg ccggcaaagg agaggccgct
     1621 atcaaggatc gtccggcgca gggcggagac ccggtccggg cccagggccg aggggtctgg
     1681 accaagggtg aagggcgcga tctcgatgcc cgtgaagccc gcagccgcga tcatcccgca
     1741 ggaggccgcg aaatccatcg ccccgaagag ctcgttgcag atggcgtacc tcatcggccc
     1801 tctcccttcg ccttgagctc ggcgacggcg tccagaaagg ccagggcgtt ctccaggggg
     1861 acgtcctcct ggatgttgtg cacggccgag atcacccagc ctcccccggg gccgagggca
     1921 gccgcgttgc ggaggagctc ggccctgacc tcggcggggc tgcccagggg cagtacgcgt
     1981 tttgtgtcca ccgccccgcc ccagaaggag agccgatccc cgaagtcccg cttgagcgcg
     2041 gccggctcca tcccggcggc cgcgtactgg acggggttca gcgcgtcgac gcccgcctcg
     2101 atgaggtcgc cgatgatttc gcgtatcgcg ccgcagcagt ggaagacgat cctcgcgtcc
     2161 ggcagcctcg atctgatggc agagaagagc cgggcgtagc gcccgatgta ggtcctgcgg
     2221 accatgtgag gcgagatgag aagggagatc tccgagccgt agtcgtcggc ctcggcgatg
     2281 cccgcggtgg ccgcccccca aaacgagacc tgggcctgcc agtaggcgag cttgtactcg
     2341 aggaggcgct cggccagctc ctcgaccccc tcgggatcgt cgtacatgtc gagatagagg
     2401 cgctccgtcc cgcggagccg cgccgacatc tcaaggaggc ccgcgcagtc ccggtcgagg
     2461 atggggaaaa ggcccagacg gtccgagtcg agccttcccc gctcgacggc cgccctcatc
     2521 ggcccgagct cgggctcggg gaagcggtag gcctccaggg catcgctgag cctgccctcg
     2581 aacagggggg cgctcgcctg gctgtagtag agctgtccct gggcccgcct ccagagtacg
     2641 ccaaagctgt ccaccgtctc gagcgatcca tcgtcccctg tcttcagccc tcccctgatc
     2701 ctgtccatgc ccaccctgag ggtgtcggag ccgagggcga ggaggagctc gggcggggga
     2761 gccgaaatgc cctggaccgt gtcggccacc cgggtctccg tgggcaggcc cttcgccccg
     2821 aggaccttgg cgaggatgcc cgtggcgatg cccgtcaccg tggtgccgca gagg
//
LOCUS       DASSQV010000207         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_674164, whole genome shotgun sequence.
ACCESSION   DASSQV010000207 DASSQV010000000
VERSION     DASSQV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674164"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1554
                     /gene="topA"
                     /locus_tag="VFL04_03600"
                     /pseudo
     CDS             1..1554
                     /gene="topA"
                     /locus_tag="VFL04_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707647.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
     gene            1538..2476
                     /locus_tag="VFL04_03605"
     CDS             1538..2476
                     /locus_tag="VFL04_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HET7838820.1"
                     /translation="MPRPNEPGGLGEGFVAYLAYLSAVRGLSPRTIESYGEDLKHYSA
                     FLGGADADTVGGRDIRSFLAALIAEGKAGSSVNRALSALRGYYRYRVRFGGLAVDPSK
                     DIEGFGVGRKLPRFLFEDEAAAFIALADGEDFRGRRDRAILEFLYSTGCRVAEAASLR
                     LDRLDLEGGTARVIGKGSKERVVFLAESARSALAAYLPLRSSLMARTGSGLKAQDEGP
                     RNVFVNSRGGPLSERGIEWLIEGYASRAGIGKRISPHVFRHSFATHLVGRGADIRVVQ
                     ELLGHSSVSTTQIYAHVDMERLRKVYEKAHPHGSGK"
     gene            2502..>2658
                     /locus_tag="VFL04_03610"
     CDS             2502..>2658
                     /locus_tag="VFL04_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HET7838821.1"
                     /translation="MALKIRSTTIIAVRRDGKVAMAGDGQVTMGATVMKSNARKVRTM
                     NEGKILVG"
BASE COUNT          498 a          923 c          872 g          365 t
ORIGIN      
        1 gtcgagaact tcatccccga ggagtactgg agcctcgagg ccgacttcag gaagggcagg
       61 ggggtcttct ccggcgagct cgtgcagttc cgcggagcca aggccgagct caagggcgag
      121 gccgcagcga aggaggtcat cgccgccatc gccgacaaga aggcgatcgt cggggaggtc
      181 agggagagcg agaagtcgct caagcccaag ccccccttca ccacctccaa gctccagcag
      241 accgcggcga accgcctggg cttcacgagc aagaagacca tgcagatcgc ccagcagctc
      301 tacgagggcg tcaacgtggg ctcgacccgc atcggcctca tctcctacat gcgcaccgac
      361 tccacccgca tctcggacag cgccatcgag gaggtgcgcg gcttcatcag ggagcggcat
      421 cccgccgagc tccccgaggc ccccatcgtg tacgcgaccg acaagaaggc ccaggacgcc
      481 cacgaggcca tcaggcccac catggtcgcc tacacccccg agtccatgaa ggaccacctg
      541 agcaaggacc aggccaggct ctacgccctc atctgggagc gcttcgtcgc gagccagatg
      601 accccggcca agatgaggtc gacgagcgtc gacgtcctcg tcggcgaggc caccttcagg
      661 gtcacctcct cgaggatcgt ggagcagggc ttctacaagg tcatcaaggt gggggcctcg
      721 aaggaggaca agggcgaggg cgccgtgccc gagctccgga gctccgagga gctcaagctc
      781 gagggcttcc ggcccgagca gcacttcacc cagggcccct cgcgctacac cgacgcctcg
      841 atcatcaagg ccctcgagga gcagggcatc ggccgcccct cgacctacgc gcccatcatc
      901 tcggtcctcc tggaccgcta atacgtcacc agggagaacc gccagctcac cccgacgacc
      961 ctcgggcgca tgatcaacga catcctcgtc gagtccttcc ccgacgtcat cgaggcgggc
     1021 ttcaccgccg gcatggaggc gaggctcgac gaggtcgagg acgacaagac cgactgggtg
     1081 ggcgtgatcc gcggcttcta cgcccccttc aagggcaagg tcgaccacgt catggagagc
     1141 ctccagtcct accgcgggag cctcgacgag aagaccgaca tcgtctgcga gaagtgcggc
     1201 aagcccatgg tgaagaagct cggccgcttc ggcttcttcc tcgcctgcac cggcttcccc
     1261 gagtgccgca acacccgctc catacccctc gccgcctgcc cgcgctgcgg gggcgacatc
     1321 gtcgcgaggc ggagggccaa gggccgggga cgcgagttct acggctgcgc gcgctatccc
     1381 gagtgcgact tcatcaccta ccacaagccg acgaactcga actgccccaa gtgcggctgg
     1441 ttcctcgtcg agaagtacga caagaagtcg ggcgggcaca aggcctgcat caacccctcc
     1501 tgcgactacc tgcacagcca cgaggaggaa ggcgagcatg cccaggccga atgagccagg
     1561 gggcctgggc gagggcttcg tggcctatct cgcctacctc tcggccgtcc gcggcctctc
     1621 gccgcggacc atcgagtcct acggagagga cctcaagcac tactccgcct tcctcggcgg
     1681 ggccgacgcc gacacggtcg gcggcaggga catcaggtcc ttcctggccg ccctcatcgc
     1741 cgagggcaag gccggctcga gcgtgaaccg ggccctctcg gccctcaggg gctactaccg
     1801 ctaccgggtc cgcttcggcg gcctggccgt cgacccctcg aaggacatcg agggcttcgg
     1861 ggtcggcagg aagctccccc gcttcctctt cgaggacgag gccgccgcct tcatcgccct
     1921 tgccgacggc gaggacttca ggggcaggcg ggacagggcc atcctcgagt tcctctacag
     1981 caccggctgc cgcgtcgccg aggccgcctc gctgcggctc gacaggctcg acctcgaggg
     2041 gggcacagcg agggtcatcg ggaagggctc gaaggagagg gtggtcttcc tcgccgaatc
     2101 ggcccgctcg gccctcgccg cctaccttcc gctgaggtcc tccctcatgg ccaggaccgg
     2161 ctcgggcctg aaggcccagg acgaggggcc gcgcaacgtg ttcgtgaact cgaggggcgg
     2221 gcccctgagc gagcgcggga tcgagtggct catcgagggc tacgcgagcc gggcgggcat
     2281 cggcaagcgc atctcccccc acgtcttcag acacagcttc gccacccacc tcgtcggccg
     2341 gggggccgac atcagggtgg tgcaggagct tctggggcac tcgagcgtct cgacgacgca
     2401 gatctacgcc cacgtcgaca tggagaggct caggaaggtc tacgagaagg cccatcccca
     2461 cgggagcggc aaatgagcca gcgcaagagc aacgaaggag catcgctttg aagatcagat
     2521 cgacgacgat catagccgtg aggcgggacg ggaaggtggc gatggccggg gacggccagg
     2581 tgaccatggg cgccacggtc atgaagagca acgcgcgcaa ggtgcggacg atgaacgagg
     2641 gcaagatcct cgtgggtt
//
LOCUS       DASSQV010000208         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2166590, whole genome shotgun sequence.
ACCESSION   DASSQV010000208 DASSQV010000000
VERSION     DASSQV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166590"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..808)
                     /locus_tag="VFL04_03615"
     CDS             complement(278..808)
                     /locus_tag="VFL04_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HET7838822.1"
                     /translation="MISKKMTDRINEQINKEMYSAYLYLAMSARMGEAGYKGISSWLT
                     VQYHEEMFHAMKFYHYLQDQGASVQLKGIAQPDFKETGVKELFQHVLEHEKAVTKSIH
                     EIMALALEEKDFATQALDQWYVNEQVEEERNAMEILQAIELLGNSPQGLYMLNVQLGK
                     RSLGVPSSFLELGGGD"
     gene            1115..>2122
                     /locus_tag="VFL04_03620"
     CDS             1115..>2122
                     /locus_tag="VFL04_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7838823.1"
                     /translation="MQNQRVSTRFSRLITPYRGFGPSLWSMFFATIINRFGDFVGPFL
                     ALYLSRALAFDAARTGLMVSVVFGASTLGAFASGRVADSIGRKRALMACQTLAALMNL
                     AVGFDYAAAWAPWLIAVGSFFRGGARPLIGAVLTDLAPKDRRKEVFGLQYWCINVGSA
                     VGPLVAAALFNGALPWLFRGDAACTLASVLLIARGVRMPAGAGAAATSLERHDERGAL
                     RAFLARPILLAFALIALASSITYSQTGFGLPLTLSALFGPLGPRYFGYLMSLNGLAVI
                     ALSVPIARLLRNRRPLACMFFSGAFYVVGFGIYAFPLGFAGFCAATLVWTIGEIVAAV
                     NM"
BASE COUNT          326 a          731 c          654 g          411 t
ORIGIN      
        1 aggccatgga acctcccggg ctggcttgga gcccctgtcg cgccaaccaa gtggtaaccc
       61 gaggctgggg acgatgtcaa aggggctggg agcgccggcc gggccgccgg agtccggcgg
      121 cccggccgac ctccttgggg gaacggggca ccgatccggg ggacagagct catcgccact
      181 tggccccgag ccccaatcgg gccgggcgcc ggagggtcct cgttccagag ccatagggga
      241 cagagctcaa agagagctcg gggtctggtc cgaggcccta gtcgccgccg ccgagctcga
      301 ggaagctgct cggtacgccc aagctccgct tcccaagctg gacgttcagc atatagaggc
      361 cctgcgggga gttgcccagg agttcgatgg cctgcaggat ctccatggca ttcctctcct
      421 cctcaacctg ttcattgacg taccactggt ccagtgcctg ggtcgcgaaa tccttctcct
      481 cgagggcgag tgccatgatc tcgtggatgc tcttggtgac ggccttctcg tgctcaagga
      541 catgctggaa gagttccttg acccccgtct ccttgaaatc cggctgggca atgcccttga
      601 gctgcaccga agccccctgg tcctggaggt agtggtagaa cttcatcgcg tggaacatct
      661 cctcgtggta ctgaaccgtg agccacgagg aaatgccttt gtagccagcc tcccccatac
      721 gggcagacat ggccaggtag aggtaggccg aatacatctc cttgttgatc tgctcgttga
      781 tcctgtccgt cattttcttg gaaatcatgc tgcgctcctt cggtttcgca ttacctgaga
      841 cttcggaatc aatgatccaa ataatcggaa cgattcctaa tgtatccatc attatccgaa
      901 aacgcaagat ccaggaggcg attgcccaaa gactgggggt gagggggaca gagctcacgg
      961 acggcggagc ttgggcccgc agaacaggat aggcagaaga gggacagagc tcggttaggc
     1021 ggtccgccgc gtcgccgagc tcccaagctt ccggaccctg gaagggggcg gcgcggttcc
     1081 catgaggtcc gtttccgcgc tagtatccgc tcccatgcaa aatcagcgag tttcgacgag
     1141 gttctcaagg ctcatcacac cctacagggg cttcgggcct tcactctggt ccatgttctt
     1201 cgcgacgatc atcaaccgct tcggcgactt cgtcggcccc ttcctcgcgc tctacctctc
     1261 gcgggccctc gccttcgacg cggcccgcac gggcctgatg gtctcggtgg tcttcggggc
     1321 ctcgaccttg ggggccttcg cctcgggcag ggtcgccgac tcgatcggcc gcaagcgcgc
     1381 cctcatggcc tgccagaccc tcgcggccct catgaacctc gcggtgggct tcgactacgc
     1441 ggccgcctgg gccccctggc tcatcgcggt cggcagcttc ttccggggcg gggccaggcc
     1501 cctcatcggc gccgtgctca ccgacctcgc gccgaaggac cgcaggaagg aggtcttcgg
     1561 gctccagtac tggtgcataa acgtcgggag cgccgtcggc cccctcgtgg cggcagccct
     1621 cttcaacggc gcccttccct ggctcttccg cggagacgcc gcctgcaccc tcgcctccgt
     1681 gctcctgatc gcgcgcggcg tcaggatgcc ggccggcgcc ggggcggcgg cgacgagcct
     1741 cgagcggcat gacgagcgcg gggcactccg cgccttcctg gcccgcccga ttctcctggc
     1801 cttcgcgctg atagccctgg ccagctccat cacctactcg cagaccggct tcggccttcc
     1861 cctaaccctc tcggccctgt tcggccccct cggcccgcgc tatttcggat acctgatgtc
     1921 gctcaacggg ctcgccgtca tcgccctcag cgtcccgatc gcgcgcctcc tcaggaacag
     1981 gaggcccctg gcctgcatgt tcttctccgg cgccttctat gtcgtgggct tcggcatcta
     2041 cgccttcccc ctgggtttcg cgggcttctg cgccgcgacc ctggtgtgga ccatcggcga
     2101 gatcgtggcc gccgtgaaca tg
//
LOCUS       DASSQV010000209         7549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2166926, whole genome shotgun sequence.
ACCESSION   DASSQV010000209 DASSQV010000000
VERSION     DASSQV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166926"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VFL04_03625"
     CDS             complement(<1..1014)
                     /locus_tag="VFL04_03625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7838824.1"
                     /translation="MTKNSKILLAALGAAVAIGIGLYLLLGPMGEARTRRNVLASASY
                     YLGKGDYDRALDLLDKLLISNASDPEAQALRDKAMSGKTGTEAAKAEETAKAEESGQK
                     AIANSLAKLSQNLKSAPPAAAPAASAARPPAENPADAAAKAKKAEDEARRKAEADAEA
                     ARKKAEAEELAKKSRELQDRMRAVNELVDKGKAAIDRGDFQGGEKLFGDAETSFPDGE
                     DKYAGQKLAEIADSLYSAAKKNPGQPADAALQESIQTARDAKKKDANNAMPYYTLGKI
                     NADLKQTDNAITELKQANSLDPKNYLYAYELGKAYFAARRYEDARQAFDATVTLNPKF
                     ESAF"
     gene            complement(1082..1786)
                     /locus_tag="VFL04_03630"
     CDS             complement(1082..1786)
                     /locus_tag="VFL04_03630"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7838825.1"
                     /translation="MKVFRVLALGSALALAAGGLSAGPFDDGERLFRQNQPKEAVPLL
                     EQAISEAGADERAWIYLAVSYQQLGRLDDAAAVLRRGVGSAQRFKHLFYFDLGNVFTL
                     QGRNAFAEDAYGQAITANPGYAPAYLNRANSRLNLKNFDGAQADYRHYLELDIASPQR
                     AAIEELLKRLGAAIAESERLQADAAAKLAADEAAKKALLDQVTSSLKAAADETTSLSA
                     GSGSVQGYGDELKIED"
     gene            1958..3298
                     /locus_tag="VFL04_03635"
     CDS             1958..3298
                     /locus_tag="VFL04_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7838826.1"
                     /translation="MGFLSWFIIRMIATPRQTKAIADQLKQGRATAAIRSAKAILAKD
                     PRNGVAHWYLGLAYLGDSKPELALMEFKAVNQISQFGPELPEVDFRTRIAALYERFNQ
                     TEEALKEHILLTKIDPGRAVHFYDAGRLFEARSRIDVAVNYLRKSIEMDERLHQAHHV
                     LGLILYRTKHPMEARSELEAAIKWNPDDFEAFFYLGKILKEGNDYTPALLSFEKAARS
                     PEFRQKALVERGGCYMSQGSLDKAIPELERAVKMIKDEAAPESLYGRYFLAMCHEKNR
                     DLDRAIEQWERIYAKKAGFRDVAEKLAQYQEYRTDDRMKDFLTCGREEFLEICKSIIQ
                     TAMSLSIRDVVEVSDGVDIIAVEGESEKWLGAKKLPRLVRFLRVSDNVDDSAVRSLID
                     QMKKLGVVRGGIITSAGFTRPALEFAENRSVELYTKEKLQEMLAQLPVATARRR"
     gene            3295..3948
                     /locus_tag="VFL04_03640"
     CDS             3295..3948
                     /locus_tag="VFL04_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7838827.1"
                     /translation="MKILCVSDQVDPVVYSPRIRERFRDVDIVLSAGDLPMEYLGFIT
                     SMLNKPLVFVSGNHDLGALSAGGPWSTAFAPDPGADHGAIDAGFRLLGQEGLIVLGLP
                     GSMLYNRGPNQYSELAMLLHILLLAPRLLLNRLLKGRAVDLVLTHAAPRGIHDRDDPC
                     HRGFRAFLWLMRAFRPRWLIHGHIHLYDLADVRITRYGDTAVINAFGHWLLDTEVEP"
     gene            3945..4889
                     /locus_tag="VFL04_03645"
     CDS             3945..4889
                     /locus_tag="VFL04_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7838828.1"
                     /translation="MSDWYANMAQEDFARARTRELLSRIADLLDPERGRLLPLEEVLA
                     ILKPGSETYVGMKAVPVDLIVGSEGRYRDFNRHFLPRREHLRSRWVRVDMAHYQDVPL
                     PAVRLYEIGGLYFVRDGNHRVSVARLRGQGSIDAEVTSLDAIVPLSPGMTLEALKRAV
                     LEYEKSRFYEKTEFGKLTGDDGLSFSTPGRYDEILEHILVHKYFVNQDLARELSFAEA
                     VWSWHENVYRPVITAIEEEGLLSRFPGRTLSDLYVFIVKHWDELKRKYGLGYPIGEAA
                     RDFGSRFGKTPGARIREALAKHCSSLLKSLGIRRSPGA"
     gene            5112..5282
                     /locus_tag="VFL04_03650"
     CDS             5112..5282
                     /locus_tag="VFL04_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838829.1"
                     /translation="MAQISKNARTSSSTQRFYCPCGGEIKMKTLFENGKVKNLAECEK
                     CKRTERRPRDFA"
     gene            complement(5337..6110)
                     /locus_tag="VFL04_03655"
     CDS             complement(5337..6110)
                     /locus_tag="VFL04_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7838830.1"
                     /translation="MLNAIIIAVIVFVFIYYDALIRFNRVFRRKRALEITEVYGARTV
                     ERIFRLFQVYAGFRIEFEDRSGTPLPERFLLVANHQSLLDIPITMGLLRSRRLRFVAK
                     RELGKGIPLVSVLLNTVGHALINRKGDAMRAMTTIDGFAKRCRRDGTCPVIFPEGTRS
                     RTGEVGPFHTAGVRRLFEEESLPVVVAAIEGGWRVAKFRDLIANLSGGSYCLRLIAVL
                     PPPKGKKETLEAVQKARELCVAAIRDMRGAGCAEVIVSE"
     gene            6274..6543
                     /gene="rpsT"
                     /locus_tag="VFL04_03660"
     CDS             6274..6543
                     /gene="rpsT"
                     /locus_tag="VFL04_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET7838831.1"
                     /translation="MAQTNKSAEKRQRQNEKRRLRNKMEKSSIRSAAKKVLVAAQAKD
                     KAAAAAALIEMSKKIDTAAGKGIVKKNTAARKKSRMQRLVNTLSA"
     gene            6616..6954
                     /locus_tag="VFL04_03665"
     CDS             6616..6954
                     /locus_tag="VFL04_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7838832.1"
                     /translation="MAGKVTKAELIEALHAGRSLNRKDIHGLIDALFEEIKTAILAGK
                     TVELRGFGTFEVKLRKGRKRARNPKTGAIVSVEDHGVASFRPGRELKKAAWTVNPETS
                     TAEPEAQAEA"
     gene            7096..>7549
                     /locus_tag="VFL04_03670"
     CDS             7096..>7549
                     /locus_tag="VFL04_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7838833.1"
                     /translation="MDTKKRILLVDDEQINLEFFEVMLTKLGFQIEKADNGADALDLV
                     KRWKPDLIILDNIMPRLSGWEVTKIIKTSEEFRDFAEVPIIMFSALDDVKDKVEGLEM
                     GADDYITKPFSVREFRSRVRAALRRAEMLGARPPAEEPIDVAGMRIDFE"
BASE COUNT         1297 a         2511 c         2452 g         1289 t
ORIGIN      
        1 gaaggccgac tcgaatttgg ggttgagggt caccgtcgcg tcgaaggcct ggcgcgcgtc
       61 ctcgtagcgc ctcgcggcga agtaggcctt gccgagctcg taggcgtaca ggtaattctt
      121 cggatcgagg ctgttggcct gcttcagctc cgtgatcgcg ttgtcggtct gtttgaggtc
      181 ggcattgatc ttgcccagcg tgtaataggg catcgcgttg ttcgcgtcct tcttcttcgc
      241 gtcgcgagcc gtctgaatcg attcctggag ggcggcgtcg gccggctgcc cggggttttt
      301 cttggccgcc gagtacagac tgtcggcgat ctcggcgagc ttctgcccgg cgtacttgtc
      361 ctcgccgtcg gggaagctcg tctcggcgtc gccgaaaagc ttctcgcccc cctggaagtc
      421 cccgcggtcg atggcggcct tgcccttgtc caccagctcg ttgaccgccc gcatcctgtc
      481 ctggagctcg cggctcttct tcgcgagctc ctccgcctcg gccttctttc gcgcggcctc
      541 ggcgtccgcc tcggccttcc tgcgcgcctc gtcctcggcc ttcttggcct tcgccgccgc
      601 gtcggcgggg ttttcggccg gtggccgcgc cgctgaggca gcgggcgccg cagccggcgg
      661 ggcggacttg aggttctggc tcagcttggc cagggagttg gctatggcct tctggccgct
      721 ctcctcggcc ttggcagtct cctcggcctt cgcggcctcg gtgcccgtct tcccgctcat
      781 ggccttgtcg cggagggcct gggcctcggg gtcagaggcg ttggagatga ggagcttgtc
      841 gaggaggtcg agggccctgt cgtagtcgcc cttgccgagg tagtagctcg ccgaggcgag
      901 gacattccgc ctcgttctcg cctcgcccat ggggccgagg aggaggtaga ggccgatgcc
      961 gatggcgact gcggccccga gggcagcgag gaggatcttc gaattcttcg tcattcagga
     1021 ctccgcatgc tgtgtggatt ccggagccgc tcgaggcggc ccgattgcct tgctccggct
     1081 ctcagtcttc gatcttgagc tcgtctccgt agccctgcac cgagcccgag cccgccgaaa
     1141 ggctggtcgt ctcgtcggct gcggccttga gcgatgaggt cacctggtcg aggagggcct
     1201 tcttggccgc ctcgtcggcc gcgagcttgg ccgcggcgtc ggcctggagc ctttcgctct
     1261 cggcgatcgc cgcgccgagc cgcttgagca gctcctcgat cgcggccctc tgcgggctcg
     1321 cgatgtcgag ctcgaggtag tgccggtagt ccgcctgggc cccgtcgaag ttcttgaggt
     1381 tgaggcgcga gttcgcccgg ttgaggtagg cgggggcata gccgggattg gcggtgatgg
     1441 cctggccgta ggcgtcctcg gcgaaggcgt tgcggccctg gagggtgaag acgttgccga
     1501 ggtcgaagta gaagaggtgc ttgaagcgtt gggcggagcc gacgccgcgg cgcaggacgg
     1561 cggcggcgtc gtcgaggcgc ccgagctgct ggtaggagac cgccagatag atccaggccc
     1621 gctcgtcggc cccggcctcg gagatggcct gctcgaggag ggggacggcc tccttgggct
     1681 ggttctgcct gaagagccgc tctccatcgt caaagggccc cgccgagagc ccccccgcgg
     1741 cgagcgcaag ggcagagcca agggccagga cccgaaaaac cttcaaaccg cgcccccctt
     1801 ccgggatttc gtgcgccaat attgggcgct ttcaaacaaa gtcgatcgac tatagtataa
     1861 taccacggga ggggttcaga gccagcggcc gcctcccgtc aggatggaat actgaatgtc
     1921 gttcgtggtg attctcatca taattctcgg agtcgctgtg ggtttcctga gttggttcat
     1981 catcaggatg atcgcgaccc cgcgccagac caaggccatc gccgaccagc tcaagcaggg
     2041 ccgggccacc gcggcgatcc gctcggcaaa ggccatcctc gcgaaggacc cgcgcaacgg
     2101 ggtcgcccac tggtacctgg gcctcgccta tctgggtgac agcaaacccg agctcgccct
     2161 catggagttc aaggccgtca accagatctc ccagttcggc cccgagctgc ccgaggtgga
     2221 cttccgcaca aggatcgccg ccctctacga gcgcttcaac cagaccgaag aggccctcaa
     2281 ggagcacatc ctcctcacca agatcgaccc gggcagggcc gtccacttct atgacgccgg
     2341 ccggctcttc gaggcccgca gccgcataga cgtcgccgtc aactacctca ggaagtcgat
     2401 cgagatggac gagaggctcc accaggccca ccatgtcctc ggcctcatcc tctaccggac
     2461 caagcacccg atggaggcgc ggagcgagct cgaggccgcc atcaagtgga accccgacga
     2521 cttcgaggcc ttcttctacc tcggcaagat cctcaaggag ggcaacgact acacccccgc
     2581 cctcctttcc ttcgagaagg cggcccgctc ccccgagttc cggcagaagg ccctcgttga
     2641 gcgggggggc tgctacatga gccagggctc cctcgacaag gccatccccg agctcgagcg
     2701 ggcggtgaag atgatcaagg acgaggccgc gcccgagtcc ctctacggcc gctatttcct
     2761 cgccatgtgc catgagaaga accgggacct cgaccgggcc atcgagcagt gggagaggat
     2821 ctacgccaag aaggccgggt tcagggacgt ggccgagaag ctcgcccagt accaggagta
     2881 ccgcaccgac gaccggatga aggacttcct cacctgcggg agggaggagt tcctcgagat
     2941 ctgcaagtcg atcatccaga cggcgatgag cctctcgatc cgagacgtgg tcgaggtctc
     3001 cgacggggtc gacatcatcg cggtcgaggg ggagtcggag aagtggctcg gggccaagaa
     3061 gctgccccgc ctcgtccgtt tcctccgcgt ctccgacaac gtcgacgact cggcggtgcg
     3121 ctccctcatc gaccagatga agaagctggg cgtcgtgagg gggggcatca tcacgagcgc
     3181 aggcttcacg cggcccgctc tggagttcgc cgagaacagg tcggtggagc tctacacgaa
     3241 ggagaagctc caggagatgc tcgcccagct gccggtcgcg accgcccgca ggcgatgaag
     3301 atcctctgcg tctccgacca ggtcgacccc gtcgtctact cgccgcggat cagggagcgg
     3361 ttccgggacg tcgacatcgt cctgagcgcc ggcgacctgc cgatggagta cctcggcttc
     3421 atcacctcga tgctgaacaa gcccctggtc ttcgtgtccg gcaaccatga cctcggggcc
     3481 ctgtcggcgg ggggaccctg gtccactgcc ttcgcgcccg accctggcgc cgaccacggg
     3541 gccatcgacg ccggtttcag gctcctgggc caggaggggc tcatcgtcct cggcctcccc
     3601 ggctccatgc tctacaaccg cggccctaac cagtacagcg agctggccat gctcctccac
     3661 atcctcctcc tcgccccccg actcctcctg aaccgcctcc tcaagggcag ggccgtcgac
     3721 ctcgtgctga cccacgccgc cccgcggggg atccacgacc gcgacgaccc ctgccacagg
     3781 ggcttccggg ccttcctctg gctcatgcgc gccttcaggc cccgctggct catccacggc
     3841 cacatccacc tctacgacct cgccgacgtc cgcataacgc gttacgggga cacggcggtg
     3901 atcaacgcat ttgggcactg gctcctcgat acggaggtcg agccgtgagc gactggtacg
     3961 cgaacatggc ccaggaggat ttcgcgcggg cgcggaccag ggagctcctg tcgcgcatag
     4021 ccgacctcct cgaccccgag cgaggacgcc tcctccccct cgaggaggtc ctcgccatcc
     4081 tgaagccggg gagcgagacc tatgtcggga tgaaggcggt gcccgtcgac ctcatcgtcg
     4141 ggagcgaggg gcgctaccgg gacttcaacc gccacttcct gccccgccgc gagcacctgc
     4201 gctcgcgctg ggtcagggtg gacatggccc actaccagga cgtgcccctg ccggcggtgc
     4261 gtctctacga gataggcggg ctctacttcg tgcgggacgg caaccaccgc gtctcggtgg
     4321 cccggctgcg cggccagggc agcatcgacg ccgaggtcac gagcctcgac gcgatcgtcc
     4381 ccttgagccc gggcatgacc ctcgaggccc tgaagcgggc tgtgctcgag tacgagaaga
     4441 gccgcttcta cgagaagacg gagttcggga agctcaccgg ggacgatggc ctctcgttca
     4501 gcacccccgg ccgatacgac gagatcctcg agcacatcct cgtgcacaag tacttcgtga
     4561 accaggacct cgcccgggag ctcagtttcg ccgaggccgt ctggtcctgg cacgagaacg
     4621 tctaccgtcc cgtcatcacg gcgatcgagg aggagggcct gctttcgcgc ttccccggcc
     4681 gcaccctctc cgacctctac gtcttcatcg tgaagcactg ggacgagctc aagcgcaagt
     4741 acggcctcgg ctaccccatc ggggaggcgg ccagggattt cggctcgagg ttcggcaaga
     4801 ccccgggggc ccggatcagg gaggccctgg ccaaacattg ctcctcgctg ctgaagagcc
     4861 tcgggataag gaggagcccc ggggcctgaa ggccgccgca ggcttggcgg taaccttcgg
     4921 gcgggagggt tgttccatat gcgtaggggc tgggaagacg gacccgccca ttcctccatt
     4981 tcgtggtgct gctcccgggg ttctcctcct tccccgggag cgcttttttc cgtcagcccc
     5041 gctgtccggt gggcagaatt gacacggagg gggcttttgg ctatcctccc gcggaggaga
     5101 aggagtaatc catggcacag atttccaaga acgctcgtac gtccagctcc acccagcgct
     5161 tctactgccc ctgcggcggc gagatcaaga tgaagacgct gttcgagaac gggaaggtca
     5221 agaacctggc cgaatgcgag aagtgcaaga ggaccgagcg ccgcccgagg gatttcgctt
     5281 aatcaagcgg gtcgcatgcg aggattccca aggggccgtc aaacgacggc cctattttat
     5341 tcgctgacaa tgacttcggc acagccggcg ccacgcatgt ccctgatggc ggccacgcag
     5401 agctcccttg ccttctgcac cgcctcgagg gtctccttct tgcccttcgg gggcggcagg
     5461 acggcgatca ggcggaggca gtagctgccg ccgctgaggt tcgcgatgag gtcgcggaac
     5521 ttggcgaccc tccagccccc ctcgatggcg gcgaccacga cgggaaggct ctcctcctcg
     5581 aaaagccgcc gcacgccggc cgtatggaat gggccgacct ccccggtgcg cgagcgggtc
     5641 ccctcgggga agatgacggg acaggtcccg tcccgtctgc agcgcttcgc gaagccgtcg
     5701 atggtggtca ttgcccgcat ggcgtcgccc ttgcggttga tgagggcatg gcccacggtg
     5761 ttgaggagga cggagacgag ggggatgccc tttcccagct cgcgtttggc gacgaagcgg
     5821 agcctgcgcg agcggaggag gcccatggtt atcgggatgt ccaggaggct ctggtggttg
     5881 gcgacgagga ggaagcgctc tggcaggggg gtcccggacc tgtcctcgaa ctcgatcctg
     5941 aaccccgcgt atacttggaa gagcctgaag atccgctcga cggtccgggc gccgtagacc
     6001 tcggttatct caagggcgcg cttccgcctg aacacccggt tgaaccggat cagggcgtca
     6061 tagtagataa acacgaagac gatcactgcg atgatgatgg cattgagcat ggtgtaccgc
     6121 cggtaaatcg ttatactatg gaatccaagg ccctgtgaag acgggccaag tattgacaac
     6181 cctcttgcgt tctgctagcc tctcatgtca gtcgccgatc cggcagtccg ggattcggcg
     6241 cgttccatac agctatcatt ggagggattt ccattggctc agactaacaa gtccgccgaa
     6301 aagcgccagc ggcagaatga gaagcggcgc ctccgcaaca agatggagaa gagcagcatc
     6361 cgttcagccg ccaagaaggt ccttgtggcc gcccaggcga aggacaaggc cgcggccgcg
     6421 gctgccctca tcgagatgag caagaagatc gacaccgccg ccggcaaggg catcgtcaag
     6481 aagaacacgg ccgcccgcaa gaagtcgcgg atgcagcggc tggtgaatac cctcagcgcc
     6541 tgaactacga ggcgccggcg tcgcccagag gcgctccgtc ggcgcttaaa cccccggttc
     6601 tcggagaaga ggatcatggc tggcaaagtc acgaaggcag agctcatcga agcgctccac
     6661 gccgggaggt cgctcaaccg caaggatatc cacggcctca tcgatgccct cttcgaggag
     6721 atcaagacgg cgatactcgc gggcaagacc gtcgagctcc gcggcttcgg gaccttcgag
     6781 gtcaagctcc gcaagggacg caagcgcgcg cggaacccca agaccggggc gatcgtgtcg
     6841 gtcgaggacc atggcgtcgc ctcgttccgc ccgggccggg agctcaagaa ggccgcctgg
     6901 accgtgaacc ccgagacctc cacggcggag cccgaggccc aggccgaggc ctaagcccca
     6961 agccctccca tcggggcccc tggccccgcc ccgctttgca ttgacaaccc aatggccctg
     7021 tgggacaatg cgcctagcca gatgagggcc gcgacgcggc ccaaactgcg caaaccaccg
     7081 gaacggaaga ccagcatgga cacaaaaaag cgcatacttc tcgtcgacga tgagcagatc
     7141 aacctcgagt tcttcgaggt gatgctcacc aagctcggct tccagatcga gaaggccgac
     7201 aacggggccg acgccctcga ccttgtgaag cgctggaagc ccgacctcat catcctcgac
     7261 aacatcatgc cccgcctctc cggctgggaa gtcaccaaga tcatcaagac cagcgaggag
     7321 ttccgggact tcgccgaggt gccgatcatc atgttctcgg ccctggacga cgtgaaggac
     7381 aaggtcgagg gcctcgagat gggcgccgac gactacatca ccaagccctt ctcggttcgc
     7441 gagttccgca gccgcgttcg cgcggcgctc cggcgcgcgg agatgctggg cgcccgcccg
     7501 cccgcggagg agccgatcga cgtcgccggg atgcgcatcg acttcgagc
//
LOCUS       DASSQV010000210         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_426360, whole genome shotgun sequence.
ACCESSION   DASSQV010000210 DASSQV010000000
VERSION     DASSQV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426360"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="VFL04_03675"
     CDS             complement(<1..1303)
                     /locus_tag="VFL04_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7838834.1"
                     /translation="MLLIIILGLIGLGLVVVVHELGHFAAARAMGVEVEAFSVGWGPK
                     LFGFRRSNTEWRISAVPIGGYCKMKGEESFRKALEDKAESFPREPGSFYGAAPWRRIV
                     IAVAGPLANILFALLVFVVVSAVGYSIRTSSNRIVLASEYHLDPSAEGKSFPADAAGL
                     KSGDRIISADGKPVADYSDVQETIALSADRAIRLGVERGGSRLDLTVTPAMDRSSGAG
                     RIGVYSWTDPVVEEVAKGSAAAIAGIETGDVVTAIDGKPVKHAIEALSYLASKPERAR
                     FTLLRNGASVDASVVLAWTETGESNLGISFRSQLKTFRAQSMGAAIAGGAAETWKTFS
                     ISFKSLGLLFRGVDLLKAVSGPARITYFVGRSASEGMKAGSTGGLALPLNFLAFLSIG
                     LFIMNLLPIPALDGGQVLLFITEIARRRPLRVITVYRFQFVG"
     gene            complement(1294..1554)
                     /locus_tag="VFL04_03680"
                     /pseudo
     CDS             complement(1294..1554)
                     /locus_tag="VFL04_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024684.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
     gene            complement(1567..>2062)
                     /locus_tag="VFL04_03685"
     CDS             complement(1567..>2062)
                     /locus_tag="VFL04_03685"
                     /inference="COORDINATES: protein motif:HMM:NF020025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET7838835.1"
                     /translation="LLQTAASESGRRILPVDSEHAALFQLVGHIGSGAIDELTITASG
                     GAFRDRPLAELAGVSADEAASHPNWSMGRKITIDSATMANKGLEVIEAVRLFGFPAER
                     VRVLIHPQSLVHALVRARDGSLYAQVSEPDMRVPIQNALTWPEALACPFGRLELAGRS
                     LDFR"
BASE COUNT          343 a          670 c          712 g          337 t
ORIGIN      
        1 cgcccacgaa ctggaaacgg tagaccgtga tcaccctgag cggccgcctt ctagcgattt
       61 cggtgatgaa cagcagcact tgtccgccgt cgagggcggg tatgggcagc aggttcatga
      121 tgaagagccc gatcgagagg aatgcgagga agttgagggg gagggcgagt cctcccgtgc
      181 tccccgcctt catgccttcg ctcgctgagc gcccgacgaa ataggtgatc cgcgcgggtc
      241 ccgagaccgc cttgagcagg tcgacgccgc ggaaaaggag gccgaggctc ttgaatgaga
      301 tggagaaggt cttccaggtc tccgccgccc ctccggcgat ggccgccccc atggactggg
      361 cgcggaaggt cttgagctgg ctcctgaagc ttatgccgag attggactcc cctgtctcgg
      421 tccaggcgag gaccaccgac gcgtcgacgc ttgcgccgtt ccggagcagg gtaaagcgcg
      481 cccgctcagg cttcgaggcc aggtaggaga gggcctcgat ggcgtgcttt acgggcttgc
      541 cgtctatcgc cgtcaccacg tcccctgtct cgatgcccgc aatggcggcc gcgctgccct
      601 tcgccacttc ctcgaccacg gggtcggtcc aggaatacac gccgattctg ccggctccgg
      661 aggatcggtc catggccgga gtgaccgtca ggtcgagccg gctgcctccc cgctccacgc
      721 cgagccttat ggccctgtcc gccgagagcg cgatcgtctc ctggacgtcg ctgtagtcgg
      781 cgacgggctt cccgtcggcc gagatgatgc ggtccccgct cttgaggccc gccgcatcgg
      841 cggggaagct cttgccctcg gcgctgggat cgaggtggta ttcgctcgcg aggacgatgc
      901 ggttggagga ggtgcggatc gaatagccga cggcggagac gacgacgaag accaggaggg
      961 cgaagaggat gttcgccaag gggccggcga ccgcgatcac gatccgccgc cagggagccg
     1021 cgccgtagaa gctgcccggc tcgcggggga aggactcggc cttgtcctcg agggccttcc
     1081 tgaaggactc ctctcccttc atcttgcagt agccgccgat gggcacggcc gagatccgcc
     1141 actcggtgtt gctgcggcgg aagccgaaga gcttgggccc ccagcctacc gaaaacgcct
     1201 caacctcgac gcccatggcc cgggcagccg cgaaatggcc gagctcgtgc acgacgacga
     1261 caaggccgag cccgatgagg ccgaggatga tgatcagcag catgaatact ccctgacggc
     1321 ggccctagcg gcttcgcgag cctccatgtc ggcatcgaat atactctcga gtcccgtcag
     1381 gcgggaaggc caggcccgct cgaggctctt cgcgacgacc ttcgcgatgt cgaggaagcc
     1441 tatctggccg cgctcgaagg ccgcgaccgc gacctcatcg gcggcgttgt aggccacggt
     1501 ccccccctct ccgatcgcga tggcctcgta ggcaagtcgg agcaggggat agcgctcctc
     1561 ttcgggttac ctgaaatcga ggctcctgcc ggcgagctcg agccgcccga aggggcaggc
     1621 gagggcctcg ggccaggtga gggcgttctg gatcgggacc cgcatgtcgg gctctgacac
     1681 ctgggcgtag agactgccgt ccctggccct tacgagggcg tgcacgaggc tctggggatg
     1741 gatgaggacg cgcaccctct ccgccggaaa gccgaaaagg cgtacggcct cgatcacctc
     1801 gaggcccttg ttggccatcg tcgccgagtc gatcgtgatc ttgcggccca tactccagtt
     1861 cgggtgcgag gcggcttcgt cggcgctgac cccggcgagc tccgcgagtg ggcggtccct
     1921 gaaggcccca cccgaggccg tgatggtgag ctcgtcaatc gcacccgatc cgatatggcc
     1981 caccagctgg aagagggcgg cgtgctcgga atcgacgggc aggatcctgc gtccggattc
     2041 ggaggcggca gtctgcagga gg
//
LOCUS       DASSQV010000211         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_550754, whole genome shotgun sequence.
ACCESSION   DASSQV010000211 DASSQV010000000
VERSION     DASSQV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550754"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VFL04_03690"
     CDS             <1..991
                     /locus_tag="VFL04_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7838836.1"
                     /translation="EKTGALLAWACLGLGALALAASALLFTYVYTTSRQLGAERPPIR
                     LVDPASGIASGADGHVRVSVSSDPHWGAPGSDAAARSAILEGIAAARPRKDALLILGD
                     NVELGMEDGPWREELTELSAALPDTPVLSLMGNHDALINGQFHYAAYFGAAATDTGEP
                     YYWSMDLGPARLIVLELLWGDESFGRRQEAWLERALASLPPGRQVIAVSHSFIYGSGY
                     VDPETGMAWYDSPGPITRVAPILERHRVALLVSGHNHYMELLEHGPTVYAVIGAMGGQ
                     SDPEPSHVSPQSRWLRRGAFGRLDLDIGPDGIALAFVDSGGAVLHRAFIPARP"
     gene            complement(992..2110)
                     /gene="hisC"
                     /locus_tag="VFL04_03695"
     CDS             complement(992..2110)
                     /gene="hisC"
                     /locus_tag="VFL04_03695"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET7838837.1"
                     /translation="MSDCIRREPMAREMFRKELGSVKPYVPGKPIDDVKREYGLERVE
                     KLASNENPIGPSPLAVEAMRGELAHLNLYPDPGCVALREAIAAELGLSPDQLVAGNGG
                     EHLLNMVATAFIGPGDEAVMSVPSFDVYAQSVGLMGGNPVQVPLKDNKHDLEAYLERI
                     GPRTKLVYLCNPNNPTGSISTRAEVEAFVSRLDEDIILLIDEAYYEYAKARADYPSGL
                     DILRRRPSTLVLRTFSKIAGIAGIRVGYLAGSKEIISELGKVKGTFYVNRLAQAAAIG
                     ALKDREHIQKTVALNAECMRLMKAWFDAKGLAYVDSAANFIFVDTGRDSMKLFEDLMR
                     RGIIVRPGALWGYRTWLRVSTGSLEQTQAFLSALGELL"
     gene            complement(2121..3179)
                     /gene="buk"
                     /locus_tag="VFL04_03700"
     CDS             complement(2121..3179)
                     /gene="buk"
                     /locus_tag="VFL04_03700"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HET7838838.1"
                     /translation="MKVLAINPGSTSTKIAVYDETGELFKKNVEHSAAELEQYPRIAD
                     QYKMRYEALARTLEESGVSAGELSAVVGRGGLLPPVKSGAYRVNESMVDRLTNRPLVD
                     HASNLGGVLAWHIAQGRGIPAYIYDPVAVDELEDIARLTGLAEVSRKSFSHALNMRAT
                     AIKCASALGRPYRELNLIVAHLGGGITLSVHSGGRMVDIVSDDEGPFSPERAGRLPWK
                     DLLELCYASDMKTMQKRLRGKGGLVSHLGTNSTLEVEAMIAKGDARAKLVYEALAYQV
                     AKAIGELATVVEGRVDRIALTGGIAHSALMTGWIKRRVAFIAPVEIYPGENELESLAN
                     GALRVLRGEEEAREYDLG"
BASE COUNT          486 a         1120 c         1154 g          562 t
ORIGIN      
        1 cgaaaagacg ggggccctcc tcgcctgggc gtgccttggc ctcggcgccc ttgccctggc
       61 ggccagcgcc ctgcttttca cctatgtcta cacaacctca agacagctcg gcgccgagcg
      121 gccgccgatc aggctcgtgg atccggcctc gggcatcgcg tcaggagccg acggccacgt
      181 cagggtctcg gtctcgtccg acccgcactg gggcgcgccc ggctccgacg ccgcggcgcg
      241 gagcgccatc ctcgagggga tagccgcggc caggccgagg aaggacgccc tcctcatcct
      301 cggggacaat gtcgagctgg gcatggagga cgggccctgg agagaggagc tcaccgagct
      361 ctcggccgcc ctgcccgaca cgcccgtcct ctccctcatg ggcaaccacg acgccctgat
      421 caacggccaa ttccactacg cagcctactt cggcgccgcg gcgacggata cgggcgagcc
      481 ctactactgg tcaatggacc tcgggccggc gaggctcatc gtgctcgagc tcctctgggg
      541 cgacgagagc ttcggccgcc ggcaggaggc ctggctcgaa agggccctgg cctccctccc
      601 cccggggaga caggtgatag ccgtatcgca cagcttcatc tacggctcgg gctacgtcga
      661 ccccgagacc gggatggcct ggtacgacag ccccgggccc ataacccggg tggccccgat
      721 cctcgagcgc cacagggtcg ccctcctggt ctcgggccac aaccactaca tggagctcct
      781 cgagcatggg ccgaccgtct acgccgtcat cggcgccatg ggcggccaga gcgatcccga
      841 gcccagccac gtctcgcccc agtcgcgctg gctacggcgg ggagccttcg gccggctcga
      901 cctcgacatt ggccccgacg gcatcgccct cgccttcgtc gactcgggcg gcgccgtcct
      961 tcaccgggcc ttcatccccg cgaggccctg atcagaggag ctcgccgagg gcggagagga
     1021 aggcctgggt ctgctcgagg ctgcccgtgc tcacgcggag ccaggtcctg tatccccaga
     1081 gggcgcccgg cctcacgatg atgccgcggc gcatcaggtc ctcgaagagc ttcattgagt
     1141 ccctgcccgt gtcgacgaag atgaagttgg ccgccgagtc gacataggcg aggcccttcg
     1201 cgtcgaacca ggccttcatc agcctcatgc actccgcgtt gagcgccacc gtcttctgga
     1261 tgtgctccct gtccttcagc gcgccgatgg ccgcggcctg ggcgaggcgg ttcacgtaga
     1321 aggtgccctt gaccttgccg agctcggaga ttatctcctt cgagccggcg aggtagccca
     1381 cccgtatgcc cgctatgccc gcgatcttgg agaaggtgcg caggaccagc gtggagggcc
     1441 gcctccgcag tatgtcgagg ccagaggggt agtcggcccg ggccttggcg tactcgtagt
     1501 aggcctcgtc gatgaggagg atgatgtcct cgtccagacg ggagacgaag gcctcgacct
     1561 cggccctggt ggagatgctg cccgtggggt tgttcgggtt gcagaggtag acgagcttgg
     1621 tgcgcggccc gatgcgctcc agataggctt cgaggtcgtg cttgttgtcc ttgagcggca
     1681 cctggaccgg atttcccccc atgaggccga ccgactgggc gtagacgtcg aagctcggga
     1741 ctgacatgac ggcctcgtcg ccgggcccga tgaaggcggt cgccaccatg ttgagcaggt
     1801 gctcgccgcc gttgccagcg acgagctggt cgggcgagag gccgagctcg gcggcgatgg
     1861 cctcgcgcag cgcgacgcag ccggggtcgg gatagaggtt gaggtgggcg agctccccgc
     1921 gcatcgcctc gacggcgagg ggcgagggcc cgatggggtt ctcgttcgag gcgagcttct
     1981 cgacccgctc gaggccgtac tcgcgcttca cgtcgtcgat cggcttgccc ggcacatagg
     2041 gcttcaccga gccgagctcc ttcctgaaca tctcacgggc catgggctct ctccttatgc
     2101 agtctgacat gcgcggcctc ctagcctagg tcgtattccc tggcttcctc ctcgccgcgg
     2161 agcaccctga gggctccgtt ggcgagggac tcgagctcgt tctctccagg atagatctcc
     2221 acgggggcga tgaaggcgac ccgcctcttg atccagccgg tcatgagggc cgaatgggcg
     2281 atgcccccgg tgagggcgat cctgtccacc ctgccctcga cgacggtcgc cagctcgccg
     2341 atcgccttgg ccacctgata ggcgagggcc tcgtagacca gcttcgcccg cgcgtcgccc
     2401 ttggcgatca tcgcctcgac ctcgagggtg gagttcgtgc ccaggtggga cacgagcccg
     2461 cccttgccgc ggagccgctt ctgcatcgtc ttcatgtcgc tcgcgtagca gagctcgagc
     2521 aggtccttcc agggaagccg ccccgcccgc tccggggaga aggggccctc gtcgtccgag
     2581 acgatgtcga ccatcctgcc gcccgagtgg acgctgaggg tgatgccccc gccgaggtgg
     2641 gcgacgatga ggttcagctc ccggtagggc cgccccaggg ctgaggcgca cttgatggcg
     2701 gtcgcgcgca tgttgagggc atgggagaag cttttgcggc tcacctcggc gaggcccgtc
     2761 agcctcgcta tgtcctcgag ctcgtccacg gccacgggat cgtagatgta ggcggggatg
     2821 ccccggccct gggcgatgtg ccaggccagg accccgccga ggttcgaggc gtggtccacg
     2881 agggggcggt tggtgaggcg gtcgaccatg gactcgttga cccggtaggc gccggacttg
     2941 acggggggca ggaggccgcc gcgcccgacc acggccgaga gctcgccagc cgagaccccg
     3001 ctctcctcga gggtgcgagc gagggcctcg tagcgcattt tgtactggtc ggcgatccgg
     3061 gggtactgct cgagctccgc ggccgagtgc tccacgttct tcttgaagag ctcgccggtc
     3121 tcgtcgtaga cggcgatctt ggtcgaggtc gagccggggt ttatggcgag gactttcatc
     3181 gctgggctcc ctttgacgct cccgcgggag ccttggatgg tctgcgtttg gcgaaggcga
     3241 ggatggcgcc gagggcgatg gagaggaagg cggccatcag cacccggtat tccggaggca
     3301 ggaggaaggt gatcgtgtgg gc
//
LOCUS       DASSQV010000212         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_53177, whole genome shotgun sequence.
ACCESSION   DASSQV010000212 DASSQV010000000
VERSION     DASSQV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53177"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VFL04_03705"
     CDS             <1..698
                     /locus_tag="VFL04_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7838839.1"
                     /translation="WDLRDAGVESLLRTSPSPTGVYCALVRPDGGRTLLVAPSAALDL
                     GLEPPNPRLFRNGGLLYIEGFLLGNRGFFMDCLRAAREASMEVALDLCSQSLVQSQRE
                     FILELLPEYCDILFANEDEFLSLVELPLAEGCSLLADLDLTLVVKRAERGAVWARGGR
                     IIDSPVRAVEPVDQTGAGDAFAAGFLVARSRGLAPERCLRLGNRIAEEVLAVPGFGVD
                     PDRLKDAAGPITA"
     gene            904..>2254
                     /locus_tag="VFL04_03710"
     CDS             904..>2254
                     /locus_tag="VFL04_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET7838840.1"
                     /translation="MPDNEIIPIDQLLPNKLPIIPLMGRPIFPGIFTPIMIGNPEDVK
                     VIEEAISADGMVGLIMIKNEFDKPKAEDLHAVGTAAKIVKRINLPDGGANIFISTLKR
                     FKVKKFLSKETPIVAAIAYLDEEGDDTDEIKALTRALLGEMKQISENNPLFSEEMRLN
                     MINIDHPGKIADFIASILNIDKNDQQKILECTNVRERMERVLIFIKKEQELLKIQKRV
                     QQEINEKIEKSQREYFLKEELKAIKGELGMPTDAKGSDYQRFKERIEGFGFEGEVKEQ
                     VEQELEKFNLMDPNSSEFIVTRNWLDLICSLPWKAAVSGDFDLQKAQDILEADHYGLK
                     DVKDRIVEYLAVRKLKKDAKGSIVCLVGPPGVGKTSVGKSIARALGKEFFRFSVGGMR
                     DEAEIKGHRRTYVGALPGKIVQGLKITKSRDPVFMIDEIDKMGVSYQGDPSSALLEAL
                     "
BASE COUNT          457 a          776 c          688 g          333 t
ORIGIN      
        1 tgtgggacct ccgcgacgcg ggggtcgaga gcctgcttcg gacctcgccc tcgccgacgg
       61 gggtctactg cgccctggtg aggcccgacg ggggcaggac cctcctcgtc gcgccgagcg
      121 ccgccctcga cctggggctc gagccgccca acccccgcct cttccgcaat ggggggctcc
      181 tgtacatcga gggcttcctc ctcggcaacc gcggcttctt catggactgc ctcagggccg
      241 cgcgcgaggc ctcgatggag gtcgccctcg acctctgcag ccagagcctc gtccagtccc
      301 agcgcgagtt catcctcgag ctcctgcccg agtactgcga catcctcttc gccaacgagg
      361 acgagttcct ctccctggtc gagctccccc tcgccgaggg ctgctccctc cttgccgacc
      421 tcgacctcac cctcgtggtg aagcgggccg agcgcggggc ggtctgggcc aggggagggc
      481 ggatcatcga cagcccggtg cgggcagtcg agcccgtgga ccagaccggg gccggcgacg
      541 ccttcgccgc gggcttcctc gtggcccgct cccgtggcct cgcccccgag cgctgtctcc
      601 gcctcggcaa ccggatcgcc gaggaggtcc tcgccgtccc cggcttcggc gtcgatcccg
      661 atcgcctaaa ggacgcggcc ggccccatca ccgcctaggg cgaaccctgg ccggattccc
      721 gcaaagttcc cggaatcccg attaagaccg gccgaatggc cggcaaaacc ttgtcaaagc
      781 cgtccaaaaa ccgtagtttt agatgcgcac accaaataca gccgaccgcg aaacctggcc
      841 agacgccacc cggcgtctcg aaagaccagg actcgccggc cacctgaacg gaggaacaaa
      901 gccatgcccg acaacgagat aatccccatc gaccagctcc tccccaacaa gcttccgata
      961 atacccctca tggggaggcc gatcttcccc ggcatcttca ccccgatcat gatcggcaac
     1021 cccgaggacg tgaaggtcat cgaggaggcc atctccgccg acgggatggt cggcctcatc
     1081 atgatcaaga acgagttcga caagcccaag gccgaggacc tgcacgccgt ggggacggcg
     1141 gccaagatcg tgaagcgcat caacctgccc gacggggggg ccaacatctt catctcgacc
     1201 ctcaagcgct tcaaggtgaa gaagttcctc tccaaggaga cgcccatcgt cgcggcgatc
     1261 gcctacctcg acgaggaggg ggacgacacc gacgagatca aggccctcac ccgcgccctt
     1321 ctcggcgaga tgaagcagat ctccgagaac aacccactgt tctccgagga gatgcggctc
     1381 aacatgatca acatcgacca ccccggcaag atcgccgact tcatcgcgag catcctcaac
     1441 atcgacaaga acgaccagca gaagatcctc gagtgcacca acgtgcgcga gcgcatggag
     1501 cgcgtcctca tcttcatcaa gaaggagcag gagctcctga agatccagaa gcgggtccag
     1561 caggagatca acgagaagat cgagaagagc cagcgggagt acttcctcaa ggaggagctc
     1621 aaggcgatca agggcgagct cggcatgccc acggacgcga agggctcgga ctaccagagg
     1681 ttcaaggaga ggattgaggg cttcggcttc gagggcgagg tcaaggagca ggtcgagcag
     1741 gagctcgaga agttcaacct catggacccc aactcgagcg agttcatcgt cacccgcaac
     1801 tggctcgacc tgatctgctc cctgccctgg aaggcggcgg tctcgggcga cttcgacctc
     1861 cagaaggccc aggacatcct cgaggccgac cactacggcc tcaaggacgt gaaggacagg
     1921 attgtcgagt acctcgcggt gcgcaagctc aagaaggacg cgaagggctc catcgtctgc
     1981 ctcgtcgggc ctcccggggt gggcaagacg agcgtgggca agtccatagc cagggccctc
     2041 ggcaaggagt tcttccgctt ctcggtcggg ggcatgaggg acgaggccga gatcaagggc
     2101 cacaggcgga cctacgtcgg ggccctgccc ggcaagatcg tccagggcct caagatcacc
     2161 aagagccggg accctgtctt catgatcgac gagatcgaca agatgggagt ctcctaccag
     2221 ggcgacccct cgagcgccct gctcgaggcc ctcg
//
LOCUS       DASSQV010000213         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_924817, whole genome shotgun sequence.
ACCESSION   DASSQV010000213 DASSQV010000000
VERSION     DASSQV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924817"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFL04_03715"
     CDS             <1..181
                     /locus_tag="VFL04_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838841.1"
                     /translation="IRALSAEGKSIMIFSSELPEVVNICDTIHLLYDGSLKSSIRNGC
                     DLDSERIRHTVTGGV"
     gene            184..1209
                     /locus_tag="VFL04_03720"
     CDS             184..1209
                     /locus_tag="VFL04_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7838842.1"
                     /translation="MGTKPDQAKTGALLGQLSNESFLTIFMVGVLVVLFAAASSIVPN
                     FFQARNVLNILTNNWFIVILGIGVTFLLITGNFDMSVGGVIALTGVLSVWFCQGANVS
                     QNALANGLGLPYPLAMALALLCAVGLGAINAFFIARLKVPSIIITLGTMMLARGTAQI
                     ITHGAQRNTSLPDEFGVVGTAAIPGTNIKLSVLIMAVLVLLAFVFERMTVAGRRIYLI
                     GANPQAARLSGVKVGRNLAALFMISSLLAGVTGILLASEYKAGVSNRATGYEFDALVI
                     ALLGGVSIAGGFGSVLGMFVGAIILSVVTSAATGLLLSPDWQFTIKGVVTFLAILAQR
                     FALDRRK"
     gene            1268..2299
                     /locus_tag="VFL04_03725"
     CDS             1268..2299
                     /locus_tag="VFL04_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7838843.1"
                     /translation="MVNQDSIRERLERSKALRADIKRVYIYVFLVLIVAAFSLVKLGQ
                     VEMFGRGHFLNPNSVVNLLRSAVPIMTLSGAFTLLMISGYIDLSVGSAMSLAAVVFAL
                     MIRSGSGLPLAFAAVLTMGLALGGINGFLVMRLRITPVIATLVTLNLYKGIAMLIVPD
                     GLSAIKGTAAAPMPAWINDYARKDVLLGLPSAFYVAILVIAVLVVLQRRTIIGKYAAA
                     IGGNRTAAELSGINAVKMVWILYAAVGAFAALAGIARASYMSLGDPLSGDNMELDCII
                     AVLLGGTAFSGGEGSVAKTIVGALIIMCVTTGLMTVIPAYWQTVAKASVLLVAVVLNH
                     LLVRVKVQA"
     gene            complement(2296..>3513)
                     /locus_tag="VFL04_03730"
     CDS             complement(2296..>3513)
                     /locus_tag="VFL04_03730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7838844.1"
                     /translation="VEPFVKRVGLERAAWLSAATVVLGLASLATAFVSNAAQLIAVRA
                     AMGLAAAMTTPGSAALAFRLFEEDRLRVRAITLISTVGLVGLAIGPTLGGLVLAVAPW
                     QVLLMANAPIAAFALIGLRSGIAADRAADLHQVPVDFVGAALATATVVLGLMAPTLFV
                     NKGAAGWLAWADAVLAMSAAILFVIRERGAAHPLLDLGLLAHPLVTGGLAFKAAASLA
                     VAGLGYMVTLQLQLDWGWKPAHAAIGMLPQVLVLVAGGAFVEPFVKRVGLERAAWLSA
                     ATVVLGLAVFALCGRLGYAWVAAALVLVAAGMRVVGVVAATNVLRGLPKSRTTIGAAL
                     ADTATELSSGIGIAVTGTILASMFTGEIAAGHWSARQTAEFREAVRAAGLCLTAAAAA
                     LVAWGITRSRRAA"
BASE COUNT          559 a         1241 c         1151 g          562 t
ORIGIN      
        1 catcagggcc ctctcggccg agggcaagtc catcatgatc ttctcatcgg agctgccaga
       61 ggtggtcaac atctgcgaca cgatccacct cctctacgac ggctccctca agtcgtcgat
      121 caggaacggc tgcgacctcg acagcgagcg gatccggcat accgtcacgg gtggagtctg
      181 agcatgggca cgaagccaga ccaggcaaaa acgggcgccc tcctggggca gctgagcaat
      241 gagtccttcc tcacgatctt catggtcggg gtcctcgtgg tcctcttcgc cgccgcgagc
      301 tcgatagtcc cgaacttctt ccaggcccgc aacgtgctca acatcctcac caacaactgg
      361 ttcatcgtga tcctcggcat cggggtcacc ttcctcctca tcaccggcaa cttcgacatg
      421 tcggtgggag gggtcatcgc cctcacgggg gtgctctcgg tgtggttctg ccagggcgcg
      481 aacgtctcgc agaacgccct tgccaacggc ctgggcctgc cctaccccct cgccatggcc
      541 ctcgccctgc tctgcgccgt ggggctaggg gcgatcaacg ccttcttcat cgcgcgcctc
      601 aaggtgccat cgatcatcat taccctgggc acaatgatgc tcgcgcgggg cacggcccag
      661 atcatcacgc acggcgccca gcggaacacg agcttgcccg acgagttcgg cgtcgtcggg
      721 accgcggcca tacccggcac gaacatcaag ctctcggtcc tcatcatggc ggtcctggtc
      781 ctcctcgcct tcgtcttcga gaggatgacg gtcgcgggcc ggcgcatcta cctcatcggc
      841 gccaaccccc aggcggccag gctctcgggc gtgaaggtcg ggcgcaacct cgccgccctc
      901 ttcatgatca gctcgctgct cgctggcgtc acgggcatcc tcctcgcctc ggagtacaag
      961 gcgggggtct cgaaccgggc gaccggctac gagttcgacg ccctggtcat cgccctcctc
     1021 gggggggtca gcatcgccgg gggcttcgga tcggtgctcg gcatgttcgt cggggcgatc
     1081 atcctctcgg tggtgacctc ggcggctacc ggcctcctcc tctcgccgga ctggcagttc
     1141 accatcaagg gcgtcgtgac cttcctggcc atcctcgccc agcgcttcgc cctggacagg
     1201 aggaaatgat ggacgcgctc gcaggccgcg cggacgcggt taagaaaggg gggctggagc
     1261 cgtgagcatg gtgaaccagg attcgatccg ggagcggctg gagaggtcca aggccctccg
     1321 cgctgacatt aagcgggtct acatctacgt cttcctcgtc ctcatcgtcg cggcgttcag
     1381 cctcgtgaag ctcggccagg tcgagatgtt cggccggggc catttcctga accccaacag
     1441 cgtcgtcaac ctcctgcgga gcgccgtgcc catcatgacc ctgtcgggag ccttcaccct
     1501 cctcatgatc tcgggctaca tcgacctttc ggtcgggagc gcgatgagcc tggcggccgt
     1561 cgtcttcgcc ctcatgatcc ggagcggctc tggcctgccc ctggccttcg cggcggtgct
     1621 cacgatgggc ctggccctcg ggggcatcaa cggcttcctc gtcatgaggc tgcgcatcac
     1681 gcccgtcatc gcgaccttgg tcaccctgaa cctctacaag ggcatcgcca tgctcatcgt
     1741 ccccgacggg ctctcggcga tcaagggcac ggctgcggcc ccgatgcccg cctggatcaa
     1801 cgactacgcc cgcaaggatg tcctcctggg cctcccctcg gccttctacg tcgcgatcct
     1861 cgtgatcgcg gtcctcgtcg tgctgcagcg caggacgatc atcggaaagt acgccgcggc
     1921 catcggcggc aaccgcacag cggccgagct ctccggcatc aacgcggtca agatggtctg
     1981 gatcctctat gccgccgtcg gagccttcgc ggccctcgcg ggcattgcca gggcgagcta
     2041 catgtccctt ggcgaccccc tctcgggcga caacatggag ctcgactgca tcatcgccgt
     2101 cctcctcggc ggcacggctt tttcgggcgg cgagggctcg gtcgcgaaga ccatcgtcgg
     2161 cgccctcatc atcatgtgcg tgaccacggg cctcatgacg gtgatccccg cctactggca
     2221 gacggtggcc aaggcgagcg tcctcctcgt cgccgtcgtg ctcaaccacc tcctcgtgag
     2281 ggtgaaggtc caggcctagg cggccctccg cgatcgggtg atcccccagg cgaccagggc
     2341 cgccgccgcc gcggtgagac agaggccggc ggccctgacc gcctcgcgga actcggccgt
     2401 ctgacgcgcg ctccagtgcc ctgcagcgat ctcacccgtg aacatggacg cgaggatggt
     2461 ccccgtgacc gcgatgccga tgcccgagct gagctcggtg gcggtgtcgg cgagggcggc
     2521 gccgatcgtg gtccggctct tcggcagacc gcggagcacg ttggtcgccg cgactacgcc
     2581 gaccacgcgc atcccggccg ccacgagcac gagggcagca gcgacccagg cgtagcccaa
     2641 gcgtccgcac agggcgaaga cggcgaggcc gagcacaacc gtggccgcgc tgagccaggc
     2701 cgcccgctcg aggccgacgc gtttcacgaa gggttcgacg aaggcgcccc cggcgacgag
     2761 gacaaggacc tggggcagca tcccgatggc tgcgtgggcc ggtttccagc cccaatcgag
     2821 ctggagctga agggtcacca tgtagccgag accggccacg gccagggagg ccgcggcctt
     2881 gaaggccagg ccgcccgtga caaggggatg ggcgagaagc ccgaggtcga ggaggggatg
     2941 ggccgcgccg cgctcgcgga tgacgaagag gatcgcagcc gacatggcca ggaccgcgtc
     3001 cgcccaggcc agccatccag ccgcgccctt gttgacgaaa agggtcggcg ccatgaggcc
     3061 cagcacgacc gtggccgtgg ccagggcggc gcccacgaag tccaccggaa cctgatgaag
     3121 gtcggcggcg cggtcggcgg cgatgcccga acgcaggccg atgagcgcga aagccgcgat
     3181 cggcgcgttc gccatgagga ggacctgcca tggtgcgacc gcgagcacga ggccgccgag
     3241 ggtcggtccg atcgccagac cgacgaggcc cactgtcgat attagggtga tcgccctgac
     3301 cctgagcctg tcctcctcga agagccggaa ggccagggcc gccgagcccg gggtcgtcat
     3361 ggcggccgcg aggcccatcg ccgcgcggac cgcgatcagc tgggctgcgt tgctgacgaa
     3421 ggccgtcgcc aggctcgcga ggccgagcac aaccgtggcc gcgctgagcc aggccgcccg
     3481 ctcgaggccg acgcgtttca cgaagggttc gac
//
LOCUS       DASSQV010000214         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_302664, whole genome shotgun sequence.
ACCESSION   DASSQV010000214 DASSQV010000000
VERSION     DASSQV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302664"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VFL04_03735"
     CDS             complement(<1..811)
                     /locus_tag="VFL04_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838845.1"
                     /translation="MRAHRPRPPMGAGGLAAFAAAVCSLALLSCSRPAPSPGFVFKVD
                     SPVLLVATGSGLRAPNSGESPFELSAPAAMASLAPNADVLTANGRLAMVAVNRVGISL
                     IEPTADGSSFTLANRPAPEHFAQRTAAGAWPRGEGFLVHLYHDPFAPAGEGASASSAL
                     LALDRNGTARLLTEPGSEGEGGRAQASQLFAIYPGRDGDWFAQLRTEDSDRVEGRYLR
                     ISAPDAPERRETEISRSQFERSLQPQLLSQAGPLAPAIERTVLLAAGGSALA"
     gene            complement(825..>2028)
                     /gene="proS"
                     /locus_tag="VFL04_03740"
     CDS             complement(825..>2028)
                     /gene="proS"
                     /locus_tag="VFL04_03740"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET7838846.1"
                     /translation="APELAVVTVGGGEELEEPLCIRPTSETIIWSKYKDWIQSWRDLP
                     ILINQWANVLRWEKRTRLFLRTAEFLWQEGHTAHETEAEAVEETVRMLEVYRSVVEEH
                     LCLPVIAGLKSEAEKFAGAVQTYCIEAMMQDKKALQAGTSHFLGQNFAKAFDVQFQTR
                     DGKLDHVWATSWGTSTRLVGAVIMTHSDDKGLVLPPSLAPEELVIVPIYKNDTKAAVL
                     AYGDRLLAALKASGVRAILDSDDSSSPGWKFAEWEMRGTPLRVEIGPRDREANKVMMV
                     RRDTGEKAPVDADAVPAKARELLDAIQRNLFERAKAFRDSNTRAVTTKAEILEFYGAD
                     SAGTAAAHGGFAEGLWCGSGVCEAALKAESKVTIRCLPLGRQEGISGSCCLCGSPARH
                     RAVFARNY"
BASE COUNT          293 a          670 c          759 g          306 t
ORIGIN      
        1 gggcgagggc tgagcccccc gccgcgagga ggactgtccg ctcgatggcg ggggcgaggg
       61 ggccggcctg ggagagcagc tgtggctgca gggagcgctc gaactgggag cgcgagatct
      121 ccgtctccct gcgctccggg gcgtcggggg ccgagatcct caggtagcgg ccctcgaccc
      181 gatcagaatc ctcggtcctg agctgggcga accagtcgcc gtcgcgcccc gggtagatgg
      241 cgaagagctg ggaggcctgg gccctcccgc cctcgccctc ggagcccggc tcagtgagga
      301 ggcgggcagt cccgttcctg tccagggcaa gcagggctga ggaggcggaa gcgccctccc
      361 cggccggggc gaagggatcg tgatagaggt ggaccaggaa gccctcgccc cttggccagg
      421 ctccggccgc cgtccgctgc gcgaaatgct cgggagcggg gcggttcgcc agggtgaagg
      481 aggagccgtc ggccgtgggc tcaatgaggg agatcccgac ccggttcacg gccaccatgg
      541 cgagccgccc gttggcggtg aggacatcgg cgttgggggc gaggctggcc atcgcggccg
      601 gagccgagag ctcgaagggg gactcgcccg agttcggggc gcggagcccc gagcccgtcg
      661 cgacgaggag gacgggcgag tcgaccttga agacgaagcc tgggctgggc gccggccgtg
      721 agcaggagag aagggccagg gagcagacgg cggccgcaaa cgcggccagg ccgcccgccc
      781 ccatgggcgg tctcgggcga tgggccctca tcgggacgaa ccgctcaata gttccgcgcg
      841 aacacggcgc gatggcgcgc gggcgagccg cagagacagc agctccccga gatcccctcc
      901 tgcctgccga ggggaagaca gcggatcgtc accttgctct cggccttgag cgcggcctcg
      961 cagacccccg agccgcacca caggccctcg gcgaagccgc cgtgggcagc tgccgtgccg
     1021 gcgctgtcgg ccccgtagaa ctcgaggatc tcggccttgg tcgtgacagc cctcgtgttc
     1081 gagtcgcgga aggccttggc cctctcgaag aggttcctct ggatcgcgtc gaggagctcc
     1141 ctcgccttgg cggggacggc gtcggcgtcg acgggggcct tctcgcccgt gtccctgcgg
     1201 accatcatca ccttgttggc ctcgcggtcg cggggcccga tctcgaccct gagcggcgtg
     1261 ccgcgcatct cccactcggc gaacttccag ccgggcgagg aggagtcgtc ggagtcgagg
     1321 atggcgcgca ccccgctcgc cttcagggcg gcgaggagcc tgtcgccgta ggcgaggacg
     1381 gctgccttcg tgtcgttctt gtagatgggg acaatcacga gctcctcggg ggcgaggctc
     1441 gggggcagga cgaggccctt gtcgtccgag tgggtcatga tcaccgcgcc gacgaggcgg
     1501 gtggaggtcc cccagctcgt ggcccagaca tggtcgagct tgccgtccct ggtttggaac
     1561 tgcacatcga aggccttggc gaagttctgc ccgaggaaat ggctcgtgcc ggcctggagg
     1621 gccttcttgt cctgcatcat ggcctcgatg cagtaggtct ggacggcgcc ggcgaacttc
     1681 tcggcctcgc tcttgaggcc ggcgatcacc ggcagacaga ggtgctcctc gaccaccgag
     1741 cggtagacct cgagcatcct caccgtctcc tcgacggcct cggcctcggt ctcgtgcgcg
     1801 gtgtggccct cctgccagag gaactcggcc gtgcggagga agagcctggt gcgcttctcc
     1861 cagcggagga cgttcgccca ctggtttatg aggatgggca gatcccgcca ggactggatc
     1921 cagtccttgt acttggacca gatgatcgtc tcgctcgtgg gcctgatgca gaggggctcc
     1981 tcgagctcct ccccgccgcc gaccgtgacg acggcgagct cgggggcg
//
LOCUS       DASSQV010000215         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2417300, whole genome shotgun sequence.
ACCESSION   DASSQV010000215 DASSQV010000000
VERSION     DASSQV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417300"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFL04_03745"
     CDS             complement(<1..827)
                     /locus_tag="VFL04_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7838847.1"
                     /translation="MRGLGALVAAAPLALALLVSLPLAADAATPSPSPSEATARIAHR
                     VLDNGLEVFVVPDHAVPLVTVCVVFRGGAVAQDAKDAGLFHLYEHMMFDGNEKYPSEA
                     AFNAALKGLGAASWNGSTGDGSIEYYITVPSERLAEGLAFWSWAVKRPVFNEAKLALE
                     KKVVLDEIGGLHADPDEIFDNALTSRLYPVAPWRKNVDGPESNIRGASVAQLEAIRDA
                     YYIPRNAALMIGGDADPDAAFALAERWFGDWRGGAAPRLALPEQGAIPSGIAILYPDP
                     "
     gene            complement(833..1183)
                     /locus_tag="VFL04_03750"
     CDS             complement(833..1183)
                     /locus_tag="VFL04_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838848.1"
                     /translation="MRKLNRQECLDAVERGDFDESIRAAAPSVAVVLTQSWCVQWQWM
                     RSYLEQIGEEPGRQIFWVEYDNEDFFEPFMAFKEDVFGNREVPYVRYYRGGQFVKASN
                     FIDKTGFLKALASA"
     gene            complement(1305..>2910)
                     /gene="pruA"
                     /locus_tag="VFL04_03755"
     CDS             complement(1305..>2910)
                     /gene="pruA"
                     /locus_tag="VFL04_03755"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HET7838849.1"
                     /translation="NLPQNERSLGYAPGSPERARLEAELERLLSAGTEVPCLIGGKEL
                     RTGRTRPIIRPDERQTELGLCHLAGASEARAAIEAALEAKRGWEAMPWEERAAVFRRA
                     AELVAGRHRARIVAATMLGQSKTVDQAEIDAACEVADFFRFGAAAMARVYAEQPADGP
                     SEWNRSSYRPLEGFVFAVSPFNFTALGANLAVAPALMGNVVVWKPASTAVLSNWVLMQ
                     IYEEAGLPPGVINFLPGKGSDISDVILADPRFAGLHFTGSTAVFRGLWKRVAEGLERY
                     VSYPRLVGETGGKNFVFIHPSADLEAARVAVIRGAYEYQGQKCSAASRLYLPKGLSRT
                     FLEGLADEARSLPVGKVDDFRNFLGAVIDEPAFDGIMGHIEAARPGSRLLAGGRGDKS
                     RGFFIEPTLLLSAEARSPAMTEEIFGPVLTAYVYDEADIDGALRLVDTSSPYALTGSV
                     FARDRAAIVRSLGLLRQAAGNLYVNDKPTGAVVGRQPFGGARASGTNDKAGTAGNLLR
                     WVSALSIKENLDPPRDWRYPSMAEPS"
BASE COUNT          464 a          942 c         1038 g          466 t
ORIGIN      
        1 ggatcgggat agaggatggc gatccccgag gggatggccc cctgctctgg cagggcgagc
       61 cgtggagcag caccgccgcg ccagtcgcca aaccagcgct cggcgagggc gaaggccgca
      121 tccggatcgg cgtcgccgcc gatcatgagg gcagcgttcc tcggaatgta ataggcgtcg
      181 cggatcgcct cgagctgggc cacgctggcg cctctgatgt tggactcggg cccgtcaacg
      241 tttttcctcc agggggccac cgggtaaagc cggctcgtca gggcgttgtc gaagatctca
      301 tcagggtcgg catggagccc gccgatctcg tcgaggacga ccttcttctc gagcgcgagc
      361 ttggcctcgt tgaagaccgg cctcttgacc gcccaggacc agaaggccag gccctcggcc
      421 agccgctcgg aggggacggt gatgtagtat tcgatggacc cgtcgcccgt cgagccgttc
      481 cagctggccg cgccgaggcc cttcagcgcc gcgttgaacg cggcctcgct ggggtacttc
      541 tcgttgccgt cgaacatcat gtgctcgtag aggtggaaga ggccggcatc cttggcgtcc
      601 tgggcgacag ccccgccccg gaaaacgacg cagacggtga ccagggggac ggcgtggtcg
      661 gggacgacga agacctcgag gccgttgtcc aggacgcgat gcgcgatcct ggccgtggcc
      721 tcggacggag aggggctcgg cgtggccgca tcggccgcga gcggaaggga caccaacagg
      781 gcgagcgcca gcggggcggc cgccaccaac gcgccgagac ccctcatccg cttcacgccg
      841 aggccagggc cttcaggaag ccggtcttgt cgatgaagtt gctcgccttc acgaactggc
      901 ccccgcggta gtagcgcaca tagggcacct cgcggttccc gaagacgtcc tccttgaagg
      961 ccatgaaggg ttcgaagaag tcctcgttgt cgtactccac ccagaagatc tggcgtcccg
     1021 gctcctcgcc gatctgctcg agataggagc gcatccactg ccactgcacg caccagctct
     1081 gggtgaggac cacggcgacc gatggggcgg ctgccctgat cgattcgtcg aaatcgccgc
     1141 gttccaccgc gtcaaggcat tcctgtctgt tcagtttcct cactatggct tccttgggcg
     1201 tcggcttgcc gggctcatct cccttggacg ggaggctgag gccctgaggc atggtaaacc
     1261 atttgtccga accggtcaaa gacaccggag cctccattgc caggtcatga gggctcggcc
     1321 atggaggggt agcgccagtc ccggggcgga tcgaggttct ccttgatgct cagggcgctc
     1381 acccagcgca gaaggttccc ggccgtcccc gccttgtcgt tcgtgcccga ggcccgcgcc
     1441 cctccgaagg gctgtctccc aacgacggcg cccgtcggct tgtcgttcac gtagaggttg
     1501 ccggcggcct gccggaggag cccgagggag cggacgatgg ccgcccggtc ccgagcgaag
     1561 accgagcccg tgagcgcata gggggagctc gtgtcgacaa gccgcagggc cccgtcgatg
     1621 tcggcctcgt cgtagacgta ggcggtgagc accggtccga agatctcctc ggtcatggcc
     1681 ggcgacctgg cttcggcgct cagaaggagg gtcggctcga tgaagaagcc ccgggacttg
     1741 tccccccggc cgccggcgag gagacggctg cctggccggg cggcctcgat gtggcccatg
     1801 atcccgtcga aggccggctc gtcgatgacg gccccaagga agttgcggaa atcgtccacc
     1861 tttcccacgg gcagggagcg ggcctcgtcg gcgaggccct cgaggaaggt tcttgagagg
     1921 cccttgggga ggtagagccg agaggccgcc gagcacttct ggccctggta ctcataggct
     1981 ccgcggatca cggcgaccct cgcggcctca aggtcggcgc tcgggtggat gaagacgaag
     2041 ttcttgcccc ccgtctcgcc gacgagcctc ggataggaga cgtagcgctc gaggccctcg
     2101 gcgacccgtt tccataggcc tcggaagacc gccgtggatc cggtgaagtg gaggccggcg
     2161 aaacgcggat cggcgaggat gacgtcggaa atatcgctcc cctttccagg gagaaaattg
     2221 atgacgccgg ggggaagacc ggcttcctcg tagatctgca tgaggaccca attcgagagg
     2281 acggcggtcg acgcgggctt ccagaccacg acgttgccca tgagggcggg ggccacggcg
     2341 aggttggccc cgagggccgt gaagttgaag ggagagacgg cgaacacgaa gccctcgagc
     2401 gggcggtagc tggagcggtt ccattcggac ggcccgtcgg ccggctgctc ggcgtagacc
     2461 ctggccatgg ctgccgcgcc gaagcggaag aaatcggcca cctcgcaggc ggcgtcgatc
     2521 tcggcctggt ccacggtctt ggactggccg agcatcgtgg cggcgacgat gcgcgcgcga
     2581 tgtctcccgg ccacgagctc ggccgccctg cggaagacgg ccgccctctc ctcccagggc
     2641 atggcctccc agccgcgctt ggcctcgagg gcggcctcga tggcggcgcg ggcctcggat
     2701 gcgccagcga ggtggcagag gccaagctcg gtctggcgct cgtcgggacg tattatgggc
     2761 cgggtgcggc ccgtgcgcag ctccttgccg ccgatcaggc aggggacttc ggtaccggcg
     2821 gagaggagcc tctcgagctc ggcctcgagc ctggcgcgct cgggtgaacc gggggcgtag
     2881 ccgaggcttc tttcgttctg gggcaggttc
//
LOCUS       DASSQV010000216         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_302799, whole genome shotgun sequence.
ACCESSION   DASSQV010000216 DASSQV010000000
VERSION     DASSQV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302799"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2256)
                     /gene="ileS"
                     /locus_tag="VFL04_03760"
     CDS             complement(<1..2256)
                     /gene="ileS"
                     /locus_tag="VFL04_03760"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET7838850.1"
                     /translation="MYKSVDPKVSFPRMEEEIVRFWEAERIFEKSISQREGAEEFVFY
                     DGPPFATGLPHFGHFVPGTIKDIVPRYKTMRGFKVDRRFGWDCHGLPVENLIEKELGL
                     NSKTDIETYGVANFNEACRASVLRYVKEWRSIMTRAGRWVDFDHDYKTMDPDYMESIW
                     WVVKSLWDKGLVYEGHYILPYCPRCATVLSNHELALGGYKDVHDPAITVRFRIRATPG
                     SYAGPALADGSTYFLAWTTTPWTLPSNLALSLGPDIDYVMVQDGAERFILAEARLEAY
                     YKEPAKLEVVWRKKGAELAGIEYEPLFPYFAETREQGAFRTVTGGHVTTSDGTGIVHT
                     APGFGEDDLEVLKGTGVPTICPVDAECRFTSEVSDYAGIFVKDADKAIMERLKAEGKL
                     VKREQILHAYPHCWRCSHPLIYRAISSWFVKIEPIKKAMLESNRKIYWLPAHIRDGRF
                     GKWLENARDWAISRNRYWGNPLPIWKCPDCGKTVCYGSRAEVEKVSGVKLDDLHKHFV
                     DKVKVPCSCGGSMSRIPEVLDCWFESGSMPYAQVHYPFENKDHFEAHFPADFICEGLD
                     QTRGWFYTLTVLAAGLFGTPSFKACVVNGLVLAEDGKKMSKSLRNFTDPSEVMNEFGA
                     DALRLFLMNSAVTRGDELCFSNEGVREVLKGLIIPLWNTYGFYVTYANIDGYTGGKEE
                     ALPSPRNPLDRWVLSLCESLVAEVTAALEAYDMQKAITPIAEFIDSLNNWYIRRSRRR
                     FWKSSGQPGSGA"
     gene            2451..>3252
                     /locus_tag="VFL04_03765"
     CDS             2451..>3252
                     /locus_tag="VFL04_03765"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HET7838851.1"
                     /translation="MKTAPLAILVALLAILPIGAQTWDAGLPAQLDALAERHWQPSLQ
                     TALGTFTYAYSGLATPFSRFLEEGLAASIGRSARLRLFNRAAAAAMDPAFRAVYADLF
                     KTGGVDALLSGRYFDEGDSVRAHLELTGLSDGVLVGTADIGIPKKAIPQGLAVAPSGS
                     SLETASSLGSLLGGSPEGELKVSVATERGQGAVYREGEDMAVLVTVNMEAWIKVYHVD
                     VNGAVQLIDPNRFAPGGKLRAGATVRIPGSGEAFAFRMGPPFGTEFIKV"
BASE COUNT          505 a         1074 c         1122 g          551 t
ORIGIN      
        1 agcccccgag cctggctggc cgctcgactt ccagaacctc cgccgcgagc ggcggatgta
       61 ccagttgtta aggctgtcga tgaactcggc gatgggcgtg atggccttct gcatgtcgta
      121 ggcctcgagg gcggcggtga cctcggccac gagggactcg cagagcgaga ggacccagcg
      181 gtccaggggg ttcctcggcg aggggagggc ctcctccttg cccccggtgt agccgtcgat
      241 gttcgcgtag gtgacgtaga agccgtaggt gttccacagg gggatgatga ggcccttgag
      301 gacctcgcgc acgccctcgt tggagaagca gagctcgtcg cccctggtga cggccgagtt
      361 catgaggaag agccggaggg cgtcggcccc gaactcgttc atgacctcgc tcggatcggt
      421 gaagttgcgc agggacttgg acatcttctt cccgtcctcg gctaggacga ggccgttgac
      481 gacgcaggcc ttgaacgagg gggtgccgaa gagcccggcc gcgaggacgg tgagggtgta
      541 gaaccagccg cgggtctggt caaggccctc gcagatgaag tcggcgggga agtgggcctc
      601 gaagtggtcc ttgttctcga aggggtaatg gacctgggcg tagggcatcg agcccgactc
      661 gaaccagcag tcaaggacct cgggtatccg gctcatcgag ccgccgcagg agcagggcac
      721 cttcaccttg tcgacgaagt gcttgtgcag gtcgtcgagc ttgacgcccg agaccttctc
      781 cacctcggcg cgggagccgt agcagaccgt cttcccgcag tcggggcatt tccagatcgg
      841 cagggggttg ccccagtagc ggttgcgcga aatcgcccag tcgcgggcgt tctccagcca
      901 cttgccgaag cgcccgtccc tgatgtgggc gggaagccag tagatcttcc ggttggactc
      961 gagcatggcc ttcttgatcg gctcgatctt cacgaaccag ctcgagatcg cgcggtagat
     1021 cagggggtgg gagcagcgcc agcagtgggg gtaggcgtgg aggatctgct cgcgcttaac
     1081 gagcttgccc tcggccttga gcctctccat gatcgccttg tcggcgtcct tgacgaagat
     1141 cccggcgtag tcgctcacct cggaggtgaa gcggcactcg gcgtcgacgg ggcagatcgt
     1201 cggcaccccg gtgcccttaa gtacctcgag gtcgtcctcg ccgaagccgg gggccgtgtg
     1261 gacgatgccg gtgccgtcgc tcgtcgtgac gtggccgccg gtgacggtgc ggaaggcccc
     1321 ctgctcgcgg gtctcggcga aataggggaa gaggggctcg tactcgatgc ccgcgagctc
     1381 ggcgcccttc ttgcgccaga ccacctcaag ctttgcgggc tccttgtagt aggcctcgag
     1441 cctggcctcg gcgaggatga agcgctcggc cccgtcctgg accatgacgt agtcgatgtc
     1501 cggccccagg gagagggcga ggttggaggg cagggtccag ggcgtcgtgg tccaggcgag
     1561 gaaataggtc gagccgtcgg ccagcgcggg acccgcgtag gagcccggcg tggccctgat
     1621 cctgaagcgg accgtgatcg ccgggtcgtg gacgtccttg tacccgccca gggcgagctc
     1681 gtggttcgag agcaccgtcg cgcaacgcgg gcagtagggg aggatgtagt ggccctcgta
     1741 gaccaggccc ttgtcccaga ggctcttcac cacccaccag atggactcca tgtagtcggg
     1801 gtccatggtc ttgtagtcgt ggtcgaagtc gacccagcgc ccggcgcggg tcatgatgga
     1861 gcgccactcc ttgacgtagc ggaggacact ggccctgcag gcctcgttga agttggccac
     1921 gccgtaggtc tcgatgtcgg tcttggagtt gaggccgagc tccttctcga tgaggttctc
     1981 cacggggagg ccgtggcagt cccagccgaa gcgccggtcg accttgaagc cgcgcatcgt
     2041 cttgtagcgc ggcacgatgt ccttgatagt cccgggaacg aagtggccga agtggggcag
     2101 gcccgtggcg aagggagggc cgtcgtagaa cacgaactcc tcggccccct cgcgctggga
     2161 tatcgacttc tcgaagatcc gctcggcctc ccagaaacgg acgatctcct cctccatcct
     2221 ggggaagctg accttcggat cgacgctctt gtacacgatg ttcctcctcg gggatgcggg
     2281 acgctccagt atgccatgga ggagggcatc gtttccagac atcctcgccc tgcccgcagc
     2341 acgcgccgag gcccctcggg cccgctcccc gcgcgggctc ggccgttccg cgcccggcgg
     2401 ggccatgcta tagttcctca gacccgcact ccgcccccag gagccccatc ttgaaaaccg
     2461 ccccgctcgc catcctcgtc gccctcctcg ccatcctccc catcggcgcc cagacctggg
     2521 acgccggcct tcccgcccag ctcgacgccc tcgcggagag gcactggcag ccgagcctcc
     2581 agaccgccct cggaaccttc acctacgcct actcggggct ggcgacgccc ttctcgcgct
     2641 tcctcgagga gggcctcgcc gcctcgatcg ggaggagcgc gaggctgcgg ctcttcaacc
     2701 gggcggcagc ggcggcgatg gacccggcct tccgcgccgt gtacgcggac ctgttcaaga
     2761 ccggcggggt cgacgccctg ctctccggcc gctacttcga cgagggcgat agcgtcaggg
     2821 cccacctcga gctcacgggg ctctcggacg gggtcctcgt cggcacggcc gacatcggca
     2881 tcccgaagaa ggcgataccc cagggcctcg ccgtcgcgcc ctccggctca tcgctcgaga
     2941 ccgcctcgag cctcggaagc ctcctcggcg gctcccctga gggggagctc aaggtctcgg
     3001 tggcgaccga gcgcggccag ggcgcggtct accgcgaggg cgaggacatg gcggtccttg
     3061 tgaccgtgaa catggaggcc tggatcaagg tgtaccacgt cgacgtgaac ggggccgtcc
     3121 agctgatcga ccccaaccgc ttcgccccgg gcggcaagct cagggccggg gccacggtcc
     3181 gcatcccggg ctcaggcgag gccttcgcct tcaggatggg gccgccattc ggcaccgagt
     3241 tcatcaaggt cc
//
LOCUS       DASSQV010000217         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_925166, whole genome shotgun sequence.
ACCESSION   DASSQV010000217 DASSQV010000000
VERSION     DASSQV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925166"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFL04_03770"
     CDS             complement(<1..760)
                     /locus_tag="VFL04_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838852.1"
                     /translation="MDASRRLSTRVVLGAFLSTIACVAVYCLFLPFSLAFDAKERVEF
                     WFRMATVGLLVDAIASYIVYRLSRPIAKVLDGMKGGLRRESAEFKKALWALDYIPNFL
                     LWLGAFAYFGAASANIGLDLARGKPADLGLIAARFALAIGWGFLNGIMTARLLNIILI
                     EAKLALGIYDVSEIAQLRKGRLSSLQSRLAASGMALFLFLIAFSGVIFYVRLRRFAEL
                     AAGGSDPAALSAAFSSSIGGGLAIMAGLLVIATGI"
     gene            complement(801..1799)
                     /locus_tag="VFL04_03775"
     CDS             complement(801..1799)
                     /locus_tag="VFL04_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7838853.1"
                     /translation="MLEWAVRDPLVEVLLYGIPALAALTFLLGWISYRGMIHYRSLAP
                     EAIVAAGLEGGTIRAETLALPWLESRLRSPYGYELAVRALRGTADRLVIMHHGISLDW
                     HGNFRYMEHFIREGWTVVAYDSRGHGETKSLEAGKGAGGRSSRPSYGVYEKFDLKAVA
                     DWALDTFAHSGGFVVLGESMGAASALQYVAIDGRADAIVADCSFSSAVAELDHRLKRS
                     FVPFFLRPAVVMVADLFCRRLEGFSLHEASPERAVLAREVPILFVHGLDDDYVPWRMA
                     VVMAESRRRRLSSALTELRLVPGARHAKSINVDPEGYSQSLGAFLSAALAGRIGRL"
     gene            1918..>2223
                     /locus_tag="VFL04_03780"
     CDS             1918..>2223
                     /locus_tag="VFL04_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838854.1"
                     /translation="MSITLADLGKAVIETEYAVRGPIVARAAELERSGKQIIYCNIGN
                     PQALQQKPLTYLRQILSLCEYPELMEKAPSAFPADVIETAKRLLAGSKHGMGAYSESK
                     "
BASE COUNT          406 a          726 c          783 g          308 t
ORIGIN      
        1 agatccccgt cgcgatcaca aggagccccg ccatgatcgc caggcccccg ccgatggatg
       61 aggagaaggc ggcggaaagg gcggccgggt ccgagccccc ggcggccagc tcggcgaaac
      121 gcctgagccg cacgtagaag atgacgccgg agaaggcgat gaggaagagg aagagggcca
      181 tgcccgaggc ggccaggcgg gactggaggc tcgagagcct gcccttgcgc agctgggcga
      241 tctcggagac gtcatagatg cccagggcca gcttcgcctc gatgaggatg atgttgagca
      301 ggcgcgccgt catgatgccg ttcaggaagc cccagcctat ggccagggcg aagcgggcgg
      361 cgatgaggcc gaggtcggcc ggcttgcccc gcgcgaggtc gaggccgatg ttcgccgagg
      421 ccgccccgaa gtacgcgaag gcgccgagcc agagcaggaa gttcgggatg tagtcgaggg
      481 cccagagcgc cttcttgaac tcggcgctct cccggcgtaa gccgcccttc atcccgtcca
      541 gcaccttcgc gatcggcctc gagaggcggt agacgatgta gctcgcgatc gcgtcgacca
      601 gaaggcccac ggtcgccatg cggaaccaga actcgacccg ctccttggcg tcaaaggcga
      661 gggagaaggg caggaagagg cagtacacgg cgacgcaggc gatggtggag aggaaggccc
      721 cgaggaccac ccgggttgag agccgtcgcg aggcatccat cgaaacctcc gcagggattg
      781 gtggaatctt ttgttagagt ttagagccga ccgattcggc ccgcaagcgc ggccgagaga
      841 aaagcgccga ggctctggct gtagccctcg gggtcgacat tgatggactt ggcgtgtctg
      901 gccccgggga cgagcctgag ctcggtgagg gccgagctca ggcgccggcg cctcgattcg
      961 gccatgacca cagccatcct ccagggtacg tagtcgtcgt ccaggccatg gacgaagagg
     1021 atggggacct cccgcgcgag gacggcgcgt tcggggctcg cctcgtggag cgaaaagccc
     1081 tcgagccgcc tgcagaagag gtcggccacc atgacgaccg cgggcctgag gaagaagggc
     1141 acgaagctgc gcttgagccg gtggtccagc tcggcgaccg cgctggagaa ggagcagtcg
     1201 gcgacgatgg cgtcggcccg gccgtcgatc gcgacgtact gcagggcgct cgcagccccc
     1261 atggactccc cgaggacgac gaagccgccg ctgtgggcga aggtgtcgag ggcccagtcg
     1321 gcgacggcct tgaggtcgaa cttctcatag accccatagc tggggcgcga gctccggccc
     1381 cctgcgccct tccccgcctc gaggctcttc gtctcgccgt ggccgcggga gtcgtaggcc
     1441 accacggtcc agccctccct gatgaagtgc tccatgtagc ggaagttgcc gtgccagtcg
     1501 agggagatgc cgtggtgcat gatcacgagg cggtcggccg tcccgcggag ggccctgact
     1561 gccagctcgt agccgtaggg cgacctgagc ctggactcga gccagggcag ggcgagggtt
     1621 tcggccctga tcgtcccgcc ctccagcccg gccgccacga tcgcctcggg ggcgaggctg
     1681 cggtagtgga tcatgccccg ataggagatc cagccgagga ggaaggtgag cgctgcgagg
     1741 gcgggtatgc cgtagaggag gacctcgacc aggggatcgc gcacggccca ctctagcacg
     1801 gggccggagc cggcgccagc gccaggggcg ggtctggcgc cggcggccat ggagcttgcc
     1861 ggttcccctt ccagagtgct agtatccccc aacgccccaa gccaaggaga tccccccatg
     1921 tcgatcacac tcgccgatct tggaaaggcc gtcatcgaga ccgaatacgc ggtccgcggg
     1981 cccatcgtcg ccagggccgc cgagctcgag cgctcgggca agcagatcat ctactgcaac
     2041 ataggcaatc cccaggccct ccagcagaag ccccttacct acctcaggca gatcctctcg
     2101 ctctgcgagt atcccgagct catggagaaa gccccctcgg ccttccctgc cgacgtcatc
     2161 gagaccgcga agcgcctcct cgccggctcc aagcacggca tgggcgcgta ttccgagtcc
     2221 aag
//
LOCUS       DASSQV010000218         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1172958, whole genome shotgun sequence.
ACCESSION   DASSQV010000218 DASSQV010000000
VERSION     DASSQV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172958"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VFL04_03785"
     CDS             <1..512
                     /locus_tag="VFL04_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838855.1"
                     /translation="EQPKRSIACACGKVRLELSGAPFLVNVCHCDDCQRGSAQIEALS
                     GAPRILDGFGGTAYALYRKDRVRIIQGRELIVDQRIEGEKDTRRALASCCNSPLFLDF
                     EPGHWLSLYQARFDAPVPPAERRVQTRFMPPGRKPDDGLPLHRGFPLVMVWKLLATRI
                     AMGRAKAPS"
     gene            complement(530..1327)
                     /locus_tag="VFL04_03790"
     CDS             complement(530..1327)
                     /locus_tag="VFL04_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7838856.1"
                     /translation="MNHSIDFSGHGRIHAETEGAGPAIVFVHADFVDGRMWDGVRSRL
                     SARHLTVAYDKLGFGRSDPAAGPVPRRRELGAVAEALGLEAFHLVGCSNGGQQALDFA
                     LEHPQRVLSLTLVNASPSGWQPQGEMPPLLGQMFAALPAGDMDAASELQLRIWFDGPD
                     RDRASFPRAAQEARSLAAVMNRSCVERGSFFLTEAQPLEAPALSRLAEVKAPTLVVDG
                     RWDWSENRRASRILAEGIPGARRVEVDTGHVPPLEDPDGFARLWREA"
     gene            1400..2401
                     /locus_tag="VFL04_03795"
     CDS             1400..2401
                     /locus_tag="VFL04_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET7838857.1"
                     /translation="MREDPLRADRLISLILALQNQGKTTASQLAGALGVSRRTILRDV
                     DALSLSGVPVLAEGGPGGGIWLDPAYRSSLTGMKEAELRTLLVAGDARLLDDLGWGQA
                     FRETQAKLRASVPRRFEPGVEQLRRRLFIDSRWWWHEAAERNLEALQAAVWEDRVVEG
                     EYESWEGGTKAVRLEAYGLAAKAGLWYFVGRREGEWRTYRVSRFASVRAAGAFARDPQ
                     FDLSVWWPAHAAEFAGEMAGFRFSLAIPEDQIRYLRGIAPGRVEVTGPWERPGWVRVE
                     LGLDSSLYAELVVVGLGRACVILDKPELVMAVVRRCREALAAQGEPQSDEGPSTAEH"
BASE COUNT          382 a          942 c          935 g          390 t
ORIGIN      
        1 aagagcagcc caagcggtcc attgcctgcg cctgcgggaa ggtgaggctc gagctcagcg
       61 gcgcgccctt cctcgtgaac gtctgccact gcgacgactg ccagcgcggc tcggcccaga
      121 tcgaggccct cagcggcgcc ccgaggatcc tcgacggctt cggcgggacg gcctacgccc
      181 tctaccgcaa ggacagggtg aggatcatcc agggccgcga gctcatcgtc gaccagagga
      241 tcgagggcga gaaggacacg aggcgggccc tggcctcctg ctgcaactcg ccgctcttcc
      301 tcgacttcga gccggggcac tggctctcgc tctatcaggc ccgcttcgac gccccggtcc
      361 ccccggcgga gcggagggtc cagacccgct tcatgccgcc gggcaggaag cccgacgacg
      421 gcctccccct ccaccggggc ttccccctcg tcatggtctg gaagctcctc gccacgcgca
      481 tcgccatggg ccgggcgaag gctccctcct gatcgggccc cctggctgat caggcctccc
      541 gccacagccg agcgaagccg tcggggtcct ccaggggcgg cacatggccc gtgtcgacct
      601 cgacccttct ggcgcccggt atcccctcgg ccaggatgcg gctcgccctg cggttctcgc
      661 tccagtccca gcggccgtcg accaccaggg tgggcgcctt cacctcggcg agccgcgaca
      721 gggccggagc ctccaggggt tgggcctcgg tcaggaagaa gctgccccgc tccacgcagc
      781 tgcggttcat gaccgcggcc agactgcggg cctcctgggc cgcccggggg aagctggccc
      841 tgtcccggtc ggggccgtcg aaccagatcc tcagctggag ctcggaggcc gcatccatgt
      901 cccccgccgg gagggcggcg aacatctgtc cgaggagggg cggcatctcg ccctggggct
      961 gccagcccga gggggaagcg ttgaccaggg tgagggacag cacccgctgc gggtgctcca
     1021 gcgcgaagtc gagggcctgc tggcctccgt ttgagcagcc cacgagatgg aaggcctcaa
     1081 ggccgagggc ctcggcgacg gctcccagct cgcggcggcg gggcactggg cccgcggcgg
     1141 gatcggagcg gccgaagccc agcttgtcgt aggccacggt caggtggcgg gccgacagac
     1201 gggagcggac cccgtcccac atccggccgt cgacaaagtc ggcatggacg aagacgatgg
     1261 cggggccggc cccttcggtc tcggcgtgga tccggccgtg gccggaaaag tcgatagagt
     1321 ggttcatggt gactccttga tgctatagtc gaccctggtg ggtgacatcc tttgtcactc
     1381 attttcaact tttttcaaaa tgagggagga cccgctgcgc gccgatcgcc tcatctccct
     1441 catcctggcc ctgcagaacc aagggaagac cacagcctcc cagctggccg gggccctggg
     1501 ggtcagccgc cggaccatcc tccgcgacgt cgacgccctg tccctctcgg gggtgcccgt
     1561 cctggccgag ggcggacccg ggggcgggat ctggctcgac ccggcctacc gcagctcgct
     1621 gacaggcatg aaggaggccg agctgcggac cctcctcgtg gccggcgacg cccggctcct
     1681 cgacgacctc ggctggggac aggccttccg cgagacccag gccaagctcc gcgcctcggt
     1741 cccgcgccgc ttcgagcccg gggtggagca gctgcggcgg cggctcttca tcgactcccg
     1801 ctggtggtgg cacgaggccg ccgagcggaa cctcgaggcc ctccaggccg ccgtgtggga
     1861 ggaccgggtg gtcgagggcg agtacgaaag ctgggaggga ggcaccaagg cggtccggct
     1921 cgaggcctac ggcctggccg ccaaggccgg gctctggtac ttcgtgggcc gccgggaggg
     1981 ggagtggcgc acctaccggg tgtcgcgctt cgcctcagtc cgggccgcag gggccttcgc
     2041 ccgcgacccc cagttcgacc tctcggtctg gtggccggcc cacgccgccg agttcgccgg
     2101 cgagatggcg ggtttccgct tctccctggc catccccgaa gaccagattc ggtatctgcg
     2161 gggcatcgcc ccgggccgcg tcgaggtgac cggcccctgg gaaaggcccg gctgggtcag
     2221 ggtcgagctc ggcctcgact catcgctcta cgccgagctc gtcgtcgtgg gcctcggcag
     2281 ggcctgcgtg atcctcgaca agcccgagct ggtcatggcc gttgtccggc gctgccgcga
     2341 ggccctggct gcacagggcg agccgcagtc cgacgagggg ccaagcacgg ccgagcactg
     2401 agggctacgc gcgaggcggc ggccggacca acgcgcgtcg gccgaccatc cgcttcaaat
     2461 ccgcgctagc aaaagtttct taattccctt gccagcgctt cggagcagcc cccgatactc
     2521 gagcctagct tctcggccgg gaagtaaaaa ctcgagcgga ggtgcacatg aaaaaagctg
     2581 tgttggccct cgccctcctt ggggctgggg cggccctggc catcgcccag ggagtggatc
     2641 cgggggagg
//
LOCUS       DASSQV010000219         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1919232, whole genome shotgun sequence.
ACCESSION   DASSQV010000219 DASSQV010000000
VERSION     DASSQV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919232"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VFL04_03800"
     CDS             complement(<1..1330)
                     /locus_tag="VFL04_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HET7838858.1"
                     /translation="MNKRDFPRVHFYDQDFVEIYDRTWAWIADCWVPGGAASGADKIK
                     FFQYPTEPAIDLMEQVFASFFLVYSNRIYAASNGLDALYGLQEPNGAIRSRYDSATGK
                     PIVTKENPEGLGLPLFAWAEYNLYHKTANKKRVKEVMPILQKHWAWVESTFRLENGLY
                     GVPVSATGMENSPRGNALYLSDFNSAMAMNALYLSALGDILNDKEASFQFKRSYFSLK
                     TRINSLMWNAEDGFYYDLDKNEKQVKTKTVGGYWPLLAEIPNEERSERLIAHLKDPKG
                     FGTEHPFPTLSADHPDYDKLGGGYRGSVVPPFTYMIIKGLEKYNQYELARDCAIRHLY
                     FVLDSMHQNGSGGDEETHRDTKPTVWEAYQPQKEGKAVWDGKADWPRAQFLAYTGLST
                     VALMIENIVGLYISLPRKTVDWIIPNLEIMGIENLSLKRNLVTILSNKSGR"
     gene            2053..2343
                     /locus_tag="VFL04_03805"
     CDS             2053..2343
                     /locus_tag="VFL04_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838859.1"
                     /translation="MIVTLYRTAADGRMHYYTIHDRQQVLDAPFALCASWRIGMGRER
                     EKLHRCETLGERDRLIRTLIGQRTRAGYKILYSFSRAGFVGETGPLLDAASL"
     gene            complement(2340..>3225)
                     /locus_tag="VFL04_03810"
     CDS             complement(2340..>3225)
                     /locus_tag="VFL04_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HET7838860.1"
                     /translation="ISSACPAVVRIVQLRFPSLIPNLVPLIPPMEAAARIVKERLHPG
                     EEGVGVFFITPCAGKVTVTRSPLGYERSALDGVIGLKDIFLPLRAAMERAPERRLARA
                     GRLGLEWARAEGESEAVGERRSISVDGIGNVIGILEALENGKLESLAYIEALACPAGC
                     VGGPLTVENPYLAKTRLRKREAEAPASPPGPSPARSIEGLGWSSPVQARPVLSLDADL
                     DRALEMAEEILVLAASLPGLDCGSCGAPSCRALAEDIVRGQAKQTDCVFKLRETIRKL
                     AGELMAMEEMQPPGLDRD"
BASE COUNT          550 a          972 c         1094 g          609 t
ORIGIN      
        1 ctctgcccga tttgttggac aggatggtga ccaggttgcg cttgagcgag aggttctcga
       61 tccccatgat ctcgaggttg gggatgatcc agtccaccgt cttcctcggc agcgagatgt
      121 acaggccgac gatgttctcg atcatgaggg cgaccgtgga gaggccggta taggcaagga
      181 actgcgccct cggccaatcg gccttgccgt cccagacggc cttgccttcc ttctgcggct
      241 gataggcctc ccagaccgtg ggtttcgtgt cccggtgcgt ctcctcgtcc cccccgctgc
      301 cgttctggtg catcgagtcg aggacgaagt agaggtggcg gatcgcgcag tcccgggcca
      361 gctcgtactg gttgtacttc tcgaggccct tgatgatcat gtaggtgaaa ggcgggacca
      421 ccgagccgcg atagcccccg ccaagcttgt cgtagtcggg atggtcggcg gagagggtcg
      481 ggaagggatg ctcggtcccg aagcccttgg ggtccttgag gtgggcgatc agtcgctccg
      541 agcgctcctc gttgggtatc tcggcgagaa ggggccagta gccgccgacg gtcttcgtct
      601 tcacctgctt ctcgttcttg tcgaggtcgt agtagaagcc gtcctcggcg ttccacatga
      661 gggagttgat cctggtcttg agggagaagt aggaccgctt gaactggaag gaggcttcct
      721 tgtcgttgag gatgtcgcct agggcggaga ggtagagggc gttcatcgcc atggccgaat
      781 tgaagtcgga gaggtagagg gcgtttccgc ggggcgagtt ctccatcccc gtcgccgaga
      841 ccgggacgcc gtacaggccg ttctcgaggc ggaaggtcga ctcgacccat gcccagtgct
      901 tctgcaagat gggcatgacc tccttcacgc gcttcttgtt ggcggtcttg tggtagaggt
      961 tgtactcggc ccaggcgaac agggggaggc cgaggccctc ggggttttcc ttcgtgacga
     1021 tcggcttgcc cgtggccgag tcatagcgcg agcggatcgc cccgttgggc tcctggagcc
     1081 catagagggc gtcgaggccg ttggaggcgg cgtagatcct gttcgaatag acgaggaaga
     1141 agctcgcgaa gacctgctcc atgaggtcga tggcgggctc ggtcggatac tggaagaact
     1201 tgatcttgtc ggccccgctt gcggcgcctc ccggcaccca gcagtcggcg atccatgccc
     1261 aggtcctatc gtatatttcg acgaaatcct gatcgtagaa gtgcacgcgc ggaaaatccc
     1321 gtttgttcaa acatgacccc ttataccttc agacggccgc ttgcgcgaaa gccgcgatgg
     1381 accgcaaact tttctatgga tttcagaaaa atggcgatta tgggctagag agccaatata
     1441 gtgtcccttc cgcgctttgt caaattgaaa ggtgttgaag ggtaggctcc cgcgagcggc
     1501 tccgcccaaa tccttcatca gcggagtctc ggggatcgtt gcatcaggcg tgcctccgcc
     1561 tcccccggtt caatcaaaaa agtactaaaa ttgggggagg ctcggcttga tttcgtgggc
     1621 tcgggctgta ttttattggt aggcttgtgt gggtcctgtc gccctacgcg cggcggcagg
     1681 aagcatggca gggatcagta aggagggcct gcgtggtgac tccgcctgag ggttccgggc
     1741 cggctccggc gaggcgatga gcatcgccct acggaggtgc cggccgcgtc ttccagcgag
     1801 cgcctgagcg ggcacaggat gccgcggacg tctcgcgaac atgccagtgg catgccaagg
     1861 atgggtcgaa ctggaagcag ttcgaaaatg cttttttcaa ggcattccac gtcgcgtaaa
     1921 caaggaacac ccggccgctg acggcgggca gcgcgacgca cgagaacaca gggggctccg
     1981 cgatattcag gtcgcggccc ccttttttat gcggataaag gcgagccggc ctttgtccga
     2041 tagagtgtgt acatgattgt caccctgtac aggacggcag ccgacggtcg gatgcactac
     2101 tacacgatcc atgaccgcca gcaggtcctc gatgcgccct tcgcgctctg cgcctcgtgg
     2161 aggatcggca tgggaaggga gcgggagaaa ctgcaccgct gcgagaccct gggcgagcgg
     2221 gaccgcctca tcaggaccct catcggccag cgcaccaggg ccggctacaa gatcctctat
     2281 agcttctcgc gcgccggctt cgtcggcgag acggggcccc tcctcgacgc agcctcgctc
     2341 tagtcccggt cgagtccggg aggctgcatc tcctccatcg ccatcagctc acccgccagc
     2401 ttccgtatgg tctcccgcag cttgaagacg caatccgtct gcttggcctg gccccggaca
     2461 atgtcctcgg caagggccct gcagctgggc gcgccgcagg agccgcagtc aaggccgggg
     2521 agtgaggccg ccaggacgag gatctcctcg gccatctcga gggcgcggtc gaggtcggcg
     2581 tccaggctca ggacgggcct cgcctggacg ggagagctcc agcccaggcc ctcgatggag
     2641 cgcgccgggc tgggcccggg cggggaggcc ggggcctcgg cctcgcgttt ccgcaggcgg
     2701 gtcttcgcga gatagggatt ctcgaccgtg agagggccgc cgacgcagcc cgccggacag
     2761 gcgagggctt cgatgtaggc gaggctctcg agcttgccgt tctcaagggc ctcaagtatc
     2821 ccgatgacgt tcccgatccc gtccaccgag atcgagcgcc gctcgccgac ggcttcgctc
     2881 tccccctcgg ccctggccca ctcgagaccc agcctccccg cgcgcgcgag tctgcgctca
     2941 ggcgccctct ccatggcggc gcgcagggga aggaagatgt ccttaaggcc gatgaccccg
     3001 tcgagggccg agcgctcgta gccgaggggg gagcgcgtca ccgtgacctt gccagcgcag
     3061 ggcgtgatga agaacacgcc cacgccctcc tcgccggggt ggagcctctc cttgacgatg
     3121 cgggcggcgg cctccatcgg cgggatgagg gggacgaggt tgggtatgag gctgggaaag
     3181 cggagctgga cgatcctgac cacggcgggg caggcgctcg aaatg
//
LOCUS       DASSQV010000220         2530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1173341, whole genome shotgun sequence.
ACCESSION   DASSQV010000220 DASSQV010000000
VERSION     DASSQV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173341"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1068)
                     /locus_tag="VFL04_03815"
     CDS             complement(157..1068)
                     /locus_tag="VFL04_03815"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET7838861.1"
                     /translation="MPKLVALDMIWDGAPHNAFTDLARHGGRWHCVFREGSDHAGRAG
                     RVRLISSTDGRSWEPAALIAERGVDLRDPKLSAAPGGGLELLMGGTRMSSGRPVGRRP
                     RICLSPDGRGWTRPQPILEEGDWLWRSTRFRGASYGISYRLPGPKRWTVHLLAAQSGS
                     AYRELLELGVPGRPNEATIRFRDDGRAVALVRRETGRARAWIGTSEPPYLDWTWSESA
                     ERVGGPNFLILPDGRMWAATRIWRRGRPLVALCGMTEDSLVPVLVLPSGGDCGYPGMV
                     LHRGHIWLSYYSSHEGKAKIYLAKIAP"
     gene            1199..1441
                     /locus_tag="VFL04_03820"
     CDS             1199..1441
                     /locus_tag="VFL04_03820"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HET7838862.1"
                     /translation="MRVSNLVPGQRFRVLRMSLSKEVGKRLADMGFTEGRCGLVVRRG
                     LIGGPMHVRILGYDLLIRRSEAAGIEVEPIAEGAEE"
     gene            1438..>2530
                     /locus_tag="VFL04_03825"
     CDS             1438..>2530
                     /locus_tag="VFL04_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HET7838863.1"
                     /translation="MSRDGAVTAAGAGASAAAEGVRPRPGAPGPSGARAIKVALAGNP
                     NAGKTTLFNALTGSHHKVGNYPGVTVEKREGRCTSGGVDFEVFDLPGIYSLTAYSMDE
                     VVARDFILDERPDVVVDVMDSTNLERHLYLCMQFQELGIPVVAALNMMDEAGAVGISI
                     DAPALGRLLGIPFVKTVGTRGLGTGELLGVVAAQANSGCGTASRRMDYGSELEPWLAR
                     IEALVASDPAFGARYPARWLSVKLLEKDANAAERLAAHPGRDAVLELVAAAIAWTESH
                     FGRDAEIVVSEQRYAYIHGAVAETVSVAGSRGRPVTEAVDRVLMNRFLGLPLFLLILW
                     GVFQATFSLGAIPQAWLEGLFGGLAGLAGR"
BASE COUNT          371 a          875 c          897 g          387 t
ORIGIN      
        1 aacagggcga cgacctggtc ggcgtcggcc ttcatgaagc ggtcggcgat gttcttcttg
       61 accgcctcga ggttgtcctt gatgtacttg tagtcgagca tggtgggcca tgatagcgcg
      121 gctcggggag cgcttcaagg agtcggctgc gcgcccctag ggtgcgatct tcgcgaggta
      181 gatcttcgcc ttcccctcgt gcgaggagta gtagctcagc cagatgtggc cgcggtggag
      241 gaccatgccc ggatagccgc agtccccgcc cgaaggcagc acgaggacgg ggacaagcga
      301 gtcctcggtc atgccgcaga gggcgaccag gggcctgccc ctgcgccaga tcctcgtggc
      361 ggcccacatg cgcccgtcgg ggaggatgag gaagttcggg cctccgaccc gctcggcgga
      421 ctcgctccag gtccagtcga ggtagggggg ctcgctcgtg ccgatccagg cccgggccct
      481 gcccgtctcc cggcggacca gggccacggc ccttccgtcg tcccggaacc tgatcgtcgc
      541 ctcgttgggc ctccccggca cgccgagctc gaggagctcg cgataggcgg aaccgctttg
      601 cgccgcgagg aggtggaccg tccatcgttt ggggccgggg agcctgtagc tgatgccgta
      661 gctcgcgccg cggaaccggg tcgagcgcca gagccagtcc ccctcctcga ggatgggctg
      721 gggccgcgtc cagccccggc cgtcggggga gaggcagatc ctcggcctgc gcccgaccgg
      781 cctccccgag ctcatcctag tgccccccat gaggagctcg aggcccccgc caggggcggc
      841 cgagagcttg gggtcgcgga ggtcgacgcc gcgctcggct atgagggcgg ccggctccca
      901 gctgcggccg tccgtggagg agatgagcct gaccctgccg gcgcggccgg cgtggtcgga
      961 gccctccctg aagacgcagt gccacctgcc cccatggcga gcgaggtcgg tgaaggcgtt
     1021 gtgcggcgcc ccgtcccaga tcatgtcgag cgcaacgagc tttggcataa cccctccttg
     1081 gaatcgccat ggtacccgaa aatcagcccg aggcaagggg cgcgccggcg gcaggggccg
     1141 gaagtgcttg accgtatgag ccgttgatgt tagagtcatc taacaaatgg ctgacaacat
     1201 gagagtctca aacctggttc cgggacagag gttcagggtg ctgcggatgt ccctgtccaa
     1261 ggaggtgggc aaacgcctcg ccgacatggg cttcaccgag gggcgctgcg ggctcgtggt
     1321 gaggcggggc ctcatcgggg gaccgatgca cgtgcgcatc ctcggctacg acctcctgat
     1381 cagacgctcc gaggccgcgg gcatcgaggt cgagccgatc gccgaggggg ccgaggaatg
     1441 agccgcgacg gagccgtcac cgcggccggg gctggggcct cggccgccgc cgagggggtt
     1501 aggccgcggc ccggcgcgcc cgggccctcc ggagccaggg cgatcaaggt cgccctggcg
     1561 ggcaacccca acgcgggcaa gaccaccctc ttcaacgccc tcacgggctc ccaccacaag
     1621 gtgggcaatt accccggggt caccgtggag aagcgcgagg ggcgctgcac atcgggcggt
     1681 gtcgacttcg aggtcttcga ccttccgggc atctacagcc tgaccgccta ctcgatggac
     1741 gaggtggtgg cgcgcgactt catcctcgac gagaggcccg acgtcgtggt cgacgtcatg
     1801 gactcgacca acctcgagcg ccacctctat ctctgcatgc agttccagga gctgggcatc
     1861 cccgtggtgg ccgccctcaa catgatggac gaggccggag ccgtcgggat ctcgatagac
     1921 gctccggccc ttgggcgcct cctcggcatc cccttcgtga agacggtggg cacccgcggc
     1981 ctcggcaccg gggagctcct cggcgtcgtg gccgcccagg ccaactccgg ctgcggtacg
     2041 gcctcgaggc ggatggacta cggcagcgag ctcgagccct ggctggccag gatcgaggcc
     2101 ctcgtcgcct ccgacccggc cttcggcgcg cgttaccccg cgcgttggct ctcggtgaag
     2161 ctcctcgaga aggacgcgaa cgcggccgag cgcctggccg cccacccagg gcgcgatgcc
     2221 gtgctcgagc tcgtcgccgc ggccatagcc tggaccgaga gccatttcgg cagggacgcc
     2281 gagatcgtcg tctccgagca gcgctacgcc tacatccacg gcgccgtcgc cgagaccgtc
     2341 agcgtggcag gctcgcgggg caggcccgtg accgaggccg tggacagggt cctcatgaac
     2401 cgcttcctcg gcctgcccct cttcctcctc atcctctggg gggtcttcca ggccaccttc
     2461 agcctgggcg ccatacccca ggcctggctc gagggcctct tcggcggcct cgcgggtctc
     2521 gccgggcggg
//
LOCUS       DASSQV010000221         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_429718, whole genome shotgun sequence.
ACCESSION   DASSQV010000221 DASSQV010000000
VERSION     DASSQV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429718"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1467)
                     /locus_tag="VFL04_03830"
     CDS             complement(4..1467)
                     /locus_tag="VFL04_03830"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7838864.1"
                     /translation="MNRETILVVEDEAFVGEEIKEDLERYGYFVPEVISSGDEVAQAI
                     AAHKPSLIIMDVRIEGSVDGIEAAYLANAEFRVPVIYLTAYSDELSLRRAAATSPEAY
                     LLKPFSERELVANVEIALSKRKTRDLDRLEVSASLLDVLDFAAVLTDAEGRIIKANRQ
                     ALTMLEAENAQAVRGERLSRFIEKKVKGPESSPFFLRTADGSNPEVVVRLEPIILADG
                     DSPGSIAFMERISEKERAHLLESAGMANSALLRLLPLPDAAGPGYSTGGFMLPSPSGA
                     GDFFDAFLISDGVVAFYSLDVMGHGALATLMAYSLHDLVPNIAEGMRRPAAGRLPPPL
                     DLVSKLNSHYNVESGESMPFFTIVFGFLDTRNGKFRLIRGGHPPVMLLPSQGGHKIYA
                     MNGMAVGAFQSYVAEEGSGVLEPGDRLLIFSDGFLDTFEGEDYGAKIGLLGQLAEDFR
                     LESLNAFVEAIKTRVLARRDSLRFQDDVSMLVIERRG"
     gene            complement(1481..>2161)
                     /locus_tag="VFL04_03835"
     CDS             complement(1481..>2161)
                     /locus_tag="VFL04_03835"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7838865.1"
                     /translation="AQRRLEALLSEKEFLIKEIHHRTKNGLQIVSSILALQSHRTKDP
                     TVILAFESMRNRIRSISLVHEKLHGSATGEYLDLRDYVGDLVRQLAASFGAESEGLRL
                     SVEAESIPSPMDFCADFGLMLTELVTNSFRHSVMPRGQGQVRVKIAREDEGAIALVED
                     DGPGFPTEMRPEKADSLGFRIVTSFARKWGCELEYSKQGSARVSIHIPARALKKAEEP
                     SRVPVERG"
BASE COUNT          351 a          679 c          757 g          374 t
ORIGIN      
        1 ccgctagcct cgcctctcga tgaccagcat gctcacgtcg tcctggaacc ggagggagtc
       61 ccttctggcg agcaccctcg tcttgatcgc ctccacgaag gcgttgaggc tctcgaggcg
      121 gaaatcctcg gcgagctggc cgagcaatcc gatcttggcc ccgtagtctt cgccctcgaa
      181 ggtgtccagg aagccgtcgg agaagatgag gaggcggtct ccgggctcga ggacacccga
      241 gccctcctcg gccacatagg actggaaggc gcccaccgcc atgccgttca tcgcgtagat
      301 cttgtgcccc ccctgtgagg gcaggagcat gacgggcgga tggccgccgc ggatgaggcg
      361 gaacttgccg ttcctcgtgt cgaggaagcc gaacacgatg gtgaagaagg gcatggactc
      421 gccggactcc acgttgtagt gggagttcag cttcgagacg aggtcgaggg ggggaggcag
      481 ccgccccgcg gccggcctgc gcatgccctc ggcgatgttg gggacgaggt catggaggga
      541 ataggccatc agggtggcga gggcgccatg gcccatgacg tccaggctgt agaaggccac
      601 cacgccatcg gagatgagga aggcgtcgaa gaagtccccc gcccccgatg ggctcgggag
      661 catgaagccg cccgtgctgt agcccgggcc ggcggcgtcg ggcaggggca gaagacggag
      721 aagggcgctg ttggccatgc cagccgactc gaggaggtgg gccctctcct tctcgctgat
      781 gcgctccatg aaggcgatgc tgcccggaga gtccccgtcc gcgaggatga tgggctcgag
      841 cctgaccacc acctcagggt tcgacccgtc ggccgtcctc aggaagaagg ggctcgattc
      901 gggtcccttc accttcttct cgatgaagcg cgagaggcgc tcgcccctga ccgcctgggc
      961 gttctcggcc tcgagcatgg taagggcctg gcggttggcc ttgatgatcc ggccctcggc
     1021 gtcggtcagg acggcagcga aatcgaggac gtcgaggagg gaggccgaga cctcgagccg
     1081 gtccaggtcg cgggtcttcc gctttgagag ggcgatctcg acgttggcca ccagctccct
     1141 ctcggagaag ggcttgagga ggtaggcctc gggcgaggtc gcggcggccc gcctcaagga
     1201 gagctcgtcg gaataggcgg tgaggtagat cacgggcacg cggaactcgg cgttcgcgag
     1261 ataggccgcc tcgatcccgt cgacgctgcc ctcgatcctg acatccatga tgatgaggga
     1321 gggcttgtgg gcggcgatcg cctgggcgac ctcgtcgccc gaggagatca cctcgggcac
     1381 gaagtagcca tagcgctcga ggtcctcctt gatctcctcg ccgacgaagg cctcgtcctc
     1441 gacgacgagg atcgtttcgc ggttcatggc cggatcctca tcagccgcgc tccaccggaa
     1501 cgcggctggg ctcctcggcc ttcttcaggg ccctggcggg gatgtggatc gagacccggg
     1561 ccgagccctg tttggaatac tccagttcgc agccccattt gcgggcgaag ctcgtcacga
     1621 tgcggaagcc gagggaatcg gccttctcgg gccgcatctc ggtcgggaat ccggggccgt
     1681 cgtcctcgac cagggctatc gccccctcgt cctcgcgggc gatcttgacc ctgacctggc
     1741 cctggcccct gggcatcacc gaatgccgga aggagttcgt cacgagctcg gtcagcatga
     1801 ggccgaaatc ggcgcagaag tccatcgggg aggggatcga ctcggcctcg acgctcaggc
     1861 gcaggccctc gctctcggcg ccgaagcttg cggccagctg ccttaccagg tcgcccacgt
     1921 agtccctcag gtccagatac tcccccgtcg ctgatccgtg gagcttctcg tgcacgaggc
     1981 tgatcgagcg gatgcggttg cgcatggact cgaaggcgag gatcaccgtc gggtccttgg
     2041 tcctgtggga ctggagggcg aggatgctcg agacgatctg gaggccgttc ttggtcctgt
     2101 ggtggatctc cttgatgagg aattccttct cgctcagcag ggcctcgagc cggcgctggg
     2161 c
//
LOCUS       DASSQV010000222         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1671580, whole genome shotgun sequence.
ACCESSION   DASSQV010000222 DASSQV010000000
VERSION     DASSQV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671580"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2111
                     /locus_tag="VFL04_03840"
     CDS             <1..2111
                     /locus_tag="VFL04_03840"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET7838866.1"
                     /translation="ATAESYSVAAAYGDARLDATVDFSASPLSRFLALPFAGSLDGTA
                     RLQGRLDRPDVGFKLRVRDLKVEGEPVSLSAEGAFDWKTLRLSALGARYRGQEIRSGS
                     ASLSLDDASSSLSLSFLGNLGGERLAFSLAAEGKSELGSGGDLSSMFGAYSLRGTVQA
                     LQYGAVKAESWPFLASRSSSGLSFRGGESSELVASYKADGSFSFVMRDPLPLRLVAEG
                     RVVGSTIEASASGIEADLGALGALLPPGAFGLQEGSATGALSIRGLVADPEISGSIDL
                     RGAVCAVKQWVPDPIGPLDGSIVAQGRSFAFFVPSAPVGSGRISVRASAGLDHWAPTN
                     LKLSVSSIEGTSLRADLAVGGVKVQGETTLDLDLELRGEIVALNMNFYLTRANVVIGS
                     EVLGQQKPANAPEAGRQTSFVNLTATVRPGRGVQIFFPSQDIPIVSGFAEPSSALLLR
                     YDEATDDFSAKGAVGIRGGTVFYIQRDFFLRSGRLVVNETAGKFDPLVTILAELRDRT
                     DSGPVLITLRADNAPISNFKPRLSSDPVMTEAQIAALLGANLLGVTAEEPVDLRKAVI
                     STSEFIPQLNVSKAFERRVRDTLGLDVFFLHTEVLQRWLIDLSDPTTTSAVDPLGRYL
                     DQTALYAGKYVADSVFVHGSARLREDPLVRASGLRLDSELGLEFDTPFGLVQWSVAPS
                     HPENLFISDQSLSITWRLSL"
     gene            2128..>2920
                     /locus_tag="VFL04_03845"
     CDS             2128..>2920
                     /locus_tag="VFL04_03845"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HET7838867.1"
                     /translation="MKRPLALLILALAASSVWAQVSGTTGPAAPQTPAPEQAQQDWFW
                     GKAIAGVQWDGLSHADRRELDAVTRNYIGKSFTEDLWLELQSKLYELDWFDRIEPTAI
                     PTDAAKTKITIKFVVTEKPWVEAVKVTGNSGLRSGEILDVVTTKANDIYNASKSRIDE
                     LAVRRLYLEKGYPDAKVSSTTRTGSSGKAVILVFSVVEGSQVAVREIRFSGNQAVSSQ
                     TLKGLMGLKEAGFLQSGTFQESKLEEDKGKIIDYYRGRGYVDAKVS"
BASE COUNT          460 a         1061 c          940 g          459 t
ORIGIN      
        1 gcgccacggc ggagagctac agcgtcgccg cggcctacgg cgacgccagg ctcgacgcca
       61 ccgtcgactt cagcgcctcc cccctgtccc gcttcctggc ccttcctttc gcgggttccc
      121 tcgacggcac ggccaggctc cagggccggc tcgacaggcc cgacgtcggc ttcaagctcc
      181 gcgtcaggga cctcaaggtc gagggcgagc cggtctccct ctcggcggag ggggccttcg
      241 actggaagac cctgcgcctt tcggccctcg gggcgcgcta caggggccag gagatccgct
      301 ccggctcggc ctcgctcagc ctcgatgacg cctcctcgag cctgagcctg agcttcctcg
      361 ggaacctcgg aggcgagagg ctcgccttca gcctcgcggc cgagggcaag tccgagctgg
      421 gctcgggagg ggacctgtcc tccatgttcg gcgcctactc cctgcgcggc accgtccagg
      481 ccctgcagta cggggcggtg aaggccgaga gctggccctt cctggccagc cgctcgagct
      541 cgggcctctc cttccggggg ggggaatcct ccgagctcgt ggcctcctac aaggccgacg
      601 gctccttctc cttcgtcatg cgggatcccc tgcccctgcg cctcgtggcc gagggcaggg
      661 tcgtcggctc gacgatcgag gcgagcgcct cgggcatcga agccgacctg ggggccctcg
      721 gcgccctcct tcctcccgga gcctttggcc tccaggaggg ctcggccacg ggcgccctca
      781 gcatccgcgg ccttgtggcc gaccccgaga tctcagggtc catcgacctc aggggcgccg
      841 tctgcgccgt gaagcagtgg gtccccgatc cgatcggccc ccttgacggg tccatcgtag
      901 cccagggcag gagcttcgcg ttcttcgtgc cctcggcccc ggtcggctcg ggccggatct
      961 cggtcagggc gagcgcgggg ctcgaccact gggcgcccac gaacctcaag ctctccgtga
     1021 gcagcatcga gggcacgagc ctcagggccg acctcgccgt gggcggcgtc aaggtccagg
     1081 gcgagaccac cctcgacctc gacctcgagc tcaggggcga gatcgtggcg ctcaacatga
     1141 acttctacct cacgcgggcc aacgtcgtca tcggctccga ggtcctgggc cagcagaagc
     1201 ccgcgaacgc acccgaggcg ggcaggcaga cgagctttgt caacctcacg gcgacggtga
     1261 ggccgggcag gggggtccag atattcttcc cgtcccagga catccccata gtctccggct
     1321 tcgccgagcc ctcgagcgcc ctcctcctgc gctacgacga ggcgaccgac gacttctcgg
     1381 cgaagggggc agtgggcatc cgcggcggca ccgtcttcta catccagcgc gacttcttcc
     1441 tcaggtcggg gcgcctggta gtcaacgaga cggcgggcaa gttcgacccc ctcgtgacca
     1501 tcctcgccga gttgcgcgac aggaccgact cgggcccggt gctcatcacg ctcagggccg
     1561 acaacgcgcc catctcgaac ttcaaacccc ggctgagctc ggaccccgtg atgaccgagg
     1621 cgcagatagc ggcccttctg ggcgcgaacc tcctcggcgt caccgccgag gagcccgtcg
     1681 acctccgcaa ggccgtcatc tcgacctccg agttcatacc ccagctcaac gtcagcaagg
     1741 ccttcgagcg gcgggtcagg gacacgctgg ggctcgacgt cttcttcctt cacaccgagg
     1801 tcctccagcg ctggctcata gacctctcgg acccgacgac gaccagcgcc gtggaccccc
     1861 tcggccgcta cctcgaccag acggccctct acgccggcaa gtacgtggcc gactcggtct
     1921 tcgtgcacgg ctcggcgcgg ctgcgcgagg accctcttgt ccgcgccagc ggtttgcgtt
     1981 tagactcgga actcggtctc gagttcgaca ctcccttcgg cctcgtccag tggagcgtcg
     2041 cgccgagcca ccccgaaaac ctgttcatca gcgaccagag cctcagcatc acctggcggc
     2101 tctcactatg atcgcgtgag gagtaccatg aagcgacccc tggccctctt gatcctcgcc
     2161 ctcgcggcgt cttcggtctg ggctcaggtc tcggggacga ccgggcctgc ggcgccccag
     2221 actccggcgc cggagcaagc ccagcaggac tggttctggg ggaaggccat cgccggcgtg
     2281 cagtgggacg gcctatccca cgccgaccgc cgcgagctcg acgccgtcac caggaactac
     2341 atcgggaagt ccttcaccga ggacctctgg ctcgagctgc agtcgaagct ctacgagctc
     2401 gactggttcg acaggatcga gcccacggcg atacccacgg acgcggccaa gacgaagatc
     2461 acgatcaagt tcgtggtcac cgagaagccc tgggtcgagg ccgtgaaggt gacggggaat
     2521 tcgggcctgc gctcgggcga gatcctcgac gtcgtcacga cgaaggccaa cgacatatac
     2581 aacgcgtcca agtcgcgcat cgacgagctg gccgtgaggc ggctctacct cgagaagggc
     2641 taccccgacg ccaaggtgag ctcgaccacg aggacgggct cctcgggcaa ggccgtgatc
     2701 ctcgtcttca gcgtggtcga gggctcccag gtggccgtcc gcgagatcag gttctcgggg
     2761 aaccaggcag tgtcctcgca gaccctcaag ggcctcatgg gcctcaagga ggccggcttc
     2821 ctccagtccg gcaccttcca ggagtccaag ctcgaggagg acaagggcaa gatcatcgac
     2881 tactaccgcg gccgcggcta cgtcgacgcg aaggtcagcg
//
LOCUS       DASSQV010000223         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1174228, whole genome shotgun sequence.
ACCESSION   DASSQV010000223 DASSQV010000000
VERSION     DASSQV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174228"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1050
                     /locus_tag="VFL04_03850"
     CDS             274..1050
                     /locus_tag="VFL04_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET7838868.1"
                     /translation="MQYKVERLERAYFALRVVFSIVVVGLGVAMVTALGKTGGSSSAA
                     MAWVFVFYGLMILGFFWFQKVYLVAYLKGNGVCVSERQFPEVFAAYKAMAESLGLKQL
                     PKLFLIQQGGALNAFAVRFSTKNYIAVYSDVFSVMTTDFDALRFIIGHELGHVKRRHM
                     SKRFWTFPSALVPFLGSAYSRRCEYTCDSIGSSLAEAGARRGLVLLAAGKELYGKVDV
                     EDYIARAQDNLSFAVALVGIFLSHPYLPRRIEHLRTAGPA"
     gene            1141..>2160
                     /locus_tag="VFL04_03855"
     CDS             1141..>2160
                     /locus_tag="VFL04_03855"
                     /inference="COORDINATES: protein motif:HMM:NF019205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET7838869.1"
                     /translation="MQLHIPTLVFIASLVFAIQTAALLVQYRVNKTYEGIGWWLTGSA
                     LQSLGFLMMLLVEVRSLILVAALANPLVVAGQILLGVGVARFLGWKEAGRVPLGLFAA
                     YLLAYAFFIFARDSIPGRALVVYAATAAVAAMMGYKLLRGRIGQFSLSARFTASVFLA
                     YGGFQAAMAVLTLALPPIASYDELPLVPMRVILFIMPTVVSTLWTFGFILMVNQRLNA
                     VNLEEREKLEKVFGEHKKAEQGIRTLLAEKELILKEVHHRVKNNMATMRSLLSLQAST
                     LKEPTAVAALNEAGSRVQSMMMLYDALYHSPSFTEMSLAHYLPSLIDEILSNFPKAAG
                     FRVEKD"
BASE COUNT          341 a          786 c          636 g          397 t
ORIGIN      
        1 agtcccgatg cccttgcctc cctgaccggc gaggcggcca gggccgccgc ccaggccttc
       61 ggcgtgagca tcgtggagga ggacaggtcg gccttcagcc ccggctaccc cgcgccaggc
      121 caccgcatgg cctagggccg cccgcggatg ggcgatcccg tggccgcggc gccctgcctc
      181 cctgactggc gcgacggctc gcgcccccgc ctttcctgtt tttcgcgcgg cggccgtggt
      241 atcatgtcgg ccaacgtgcg gaaggaggca gagatgcagt acaaggtcga aaggctcgag
      301 cgggcgtatt tcgccctcag ggtggtcttc agcatcgtgg tggtcgggct cggcgtcgcc
      361 atggtcaccg ccctcggcaa gacagggggc tcctcaagcg cggccatggc ctgggtcttc
      421 gtgttctatg gcctcatgat cctgggcttc ttctggttcc agaaggtcta cctcgtcgcc
      481 tacctcaagg ggaacggggt ctgcgtctcc gagcggcagt tccccgaagt ctttgctgcc
      541 tacaaggcca tggccgagag cctggggctc aaacagctgc cgaagctctt cctcatccag
      601 cagggcggag ccctgaacgc cttcgccgtc aggttctcca ccaagaacta catcgctgtc
      661 tattcggacg tcttctccgt catgaccacg gatttcgacg ccctgaggtt catcatcggc
      721 cacgagctcg gccacgtgaa gcgcaggcac atgtccaagc gcttctggac cttcccctcg
      781 gcactggtcc ccttcctcgg ctcggcatat tcgcggcgct gcgaatacac ctgcgacagc
      841 atcggctcct ccctagccga ggccggggcg aggaggggcc tcgtcctcct cgcagccggc
      901 aaagagctgt acgggaaggt cgacgtcgag gactacatcg cgagggccca ggacaacctc
      961 tccttcgcgg tggctctcgt cggcatcttt ctgagccatc cctacctgcc ccggagaatc
     1021 gagcacctga ggaccgctgg acctgcctag gccccgaccg agagacagcc ggctcattct
     1081 ctgatagcct atggttcctc cccgggccag ccctccgccc ggggaagaac agaggcgccg
     1141 atgcagctcc atatcccgac cctcgtcttc atcgcaagcc tcgtcttcgc gatccagacc
     1201 gcagccctcc tcgtccagta ccgggtgaac aagacctacg agggcatcgg ctggtggctc
     1261 accgggtcgg ccctccagtc cctcggcttc ctcatgatgc tcctcgtcga ggtcaggtcc
     1321 ctcatcctcg tcgccgccct ggccaatccc ctggtcgtcg ccggccagat cctcctcggc
     1381 gtcggggtcg cgcgcttcct gggctggaag gaggccggcc gcgtccccct cggcctcttc
     1441 gcggcctatc tgctcgccta cgccttcttc atcttcgccc gggacagcat ccccggccgc
     1501 gcccttgtcg tgtacgcggc caccgccgcc gtggcggcca tgatggggta caagctgctg
     1561 cgcgggagga taggccagtt ctcgctctcg gcccgattca cggcctcggt cttcctcgcc
     1621 tatgggggct tccaggcagc catggcggtc ctgaccctcg ccctccctcc catcgcctcc
     1681 tacgacgagc tgcccctcgt gcccatgcgg gtcatccttt tcatcatgcc caccgtcgtc
     1741 agcacgctct ggaccttcgg cttcatcctc atggtcaacc agaggctgaa cgcggtgaac
     1801 ctcgaagaga gggagaagct cgagaaggtc ttcggcgagc acaagaaggc cgagcagggc
     1861 atcaggaccc tcctggccga gaaggagctc atcctcaagg aggtccacca ccgggtcaag
     1921 aacaacatgg ccacgatgcg cagcctcctc tccctccagg cgagcaccct caaggagccc
     1981 acggcagtcg cggccctgaa cgaggcggga agccgggtcc agagcatgat gatgctctac
     2041 gacgccctct atcattcccc gagcttcacc gagatgtccc tcgcccacta cctcccctcc
     2101 ctcatcgacg agatcctgtc gaacttcccc aaggccgccg ggttcagggt ggagaaggac
//
LOCUS       DASSQV010000224         4710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1422956, whole genome shotgun sequence.
ACCESSION   DASSQV010000224 DASSQV010000000
VERSION     DASSQV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422956"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4710)
                     /locus_tag="VFL04_03860"
     CDS             complement(<1..>4710)
                     /locus_tag="VFL04_03860"
                     /inference="COORDINATES: protein motif:HMM:NF033510.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7838870.1"
                     /translation="PTELVQASFTGAAVSIAAAKEGRSAPTKLIGKTQLGHEFSAGPF
                     VFVTDLDPPQIGIDSPAQGAWLRDRLTVSGTASDPGSIAKLSWRILPEGKSSEIKGGA
                     DGRYSFAVSAADLAGSTGSATIEVEATDASGNTGRAFLALGIAGEPPKASFLSPERDA
                     EVWGPEDIAATVSSSSGLASVEYSEDGKAFTPVVFRDGYFAHRADLAAHPQAVYRLTD
                     RAGGSALVRPPVRIVAAPKPDSAGDEVSVEPAASEGRLELAGQTGGRKLSVLLPSLPE
                     PGAAEAAERFARKLLVSGPVGFKGSMAGPEGSAPRTVSLSLDGGASYAVLATAKDRKT
                     AQASLGISFSLDPAKLKGETRLILKTEDFDGNSGYATLYLVIDTGAPTISLLSPQTSS
                     SNAPGPIPLCLRIEDARGLASAELSLGGEKTSLEAAEGGRYFARILDPSASAKTGGIV
                     ATVTARDAVGNASTMAFKIGYDAAADAPKAILTLGDSGKYLGVGSLLSCRSDDDDGPA
                     SLVLSIDGKQLATSEGGSIALALPRLDAGKHLVSLLARDAGGRTTEAKREILALGSGP
                     SIGSIELGDQKAKTAWVPGLPLAIVQGSVLAGSVEAANGLSSIECRIGDRQLPVALGK
                     PAPSGPIPFTLALPQLPYGPASISLTAKDAAGLSVTRSYLIVAILPAAGGEDDADALR
                     FEDARIGAAGDPSAARAVLLSPGEALVGRFKGRDISSVSLKPATELIEASFEGPIVSL
                     KAKAQGMTGPTTVRVQTIDGDVFEWGQAVFTVDAGPPSLELAGPADSIWVKDRLKVSG
                     IARDELGLGYVGYSIDGGEFQELSRAAVAAPAPAAPAAPTGSRRKADDYWPQPNAKAP
                     PPPPAPVLPPPPDPREYRFEADLALSQADGAHRVEILARDLAGKETKELRLFLKDATP
                     PLITQVLPEPGERVNGLLTFIGEASDNGRLAGLVFTAPGSATAAGSAAAPGPATAAGP
                     ATAAGSATAGSAAALPSAAAQGAAAAGAVGPAVEVQGLSTFRASLDANALDFPLPAGA
                     GFVATDRAGNSAVFSPALAVDKEADLPVADIHAPLENEVLRQDFVMSGVAFDDDGVKA
                     VSYRIDGGEWKRLELSGQSFSLPIGILATGDNEHLVETKAEDVYGIEGKVTSRKFRIS
                     TEEPFARLVSPPSTKVLSGIVELSGTATDANSVSAMALSFDSGASFQKPEGSSAWSYR
                     FDTRLLADGARAIALRPVDGYDTVGFTAALLTVDNTPPETQLDLPMDGSEAARELLVS
                     GRVSDNLGLASCRIELLPAKQTQSVLSSDIGTGRIVRTKLDISALSPGLYTVRLIARD
                     LAGNESLSSRDIRITGALPEDRIEILFPLPGSTGTGALRVSGKAEVAGGAAMVEAFLD
                     GRTAGSAVPDPQGFFNLDLAPEGLTEGTHKLSVRAQGPDGRLAQSPERELAWTRLGPW
                     VRIDSLKTGDFVPYRPFLKGKAGMNVDAPDPEDRKAVAEFKKAYKDRAVSMVELSLNG
                     GLSYQPAEGKESWRFRLETQDYREGQLHIVVRAHYGDGSTASCSTILSLDKTPPKVEI
                     LSPREGEPQNQNILVIGS"
BASE COUNT          636 a         1553 c         1783 g          738 t
ORIGIN      
        1 cgaaccgata acgaggatgt tctggttctg gggctccccc tcgcgcggcg agagaatctc
       61 gaccttgggc ggggtcttgt ccagggacag gatcgtggag cacgaggctg tgctgccgtc
      121 gccgtagtgg gcccggacga cgatgtggag ctggccctcg cggtagtcct gggtctcgag
      181 gcggaaacgc cagctctcct tgccctcggc cggctgatag ctcagaccgc cgttcaggct
      241 cagctcgacc atggacaccg ctcggtcctt gtaggccttc ttgaactcgg cgaccgcctt
      301 cctgtcctcg gggtcggggg catcgacgtt catcccggcc ttgcccttca ggaagggcct
      361 gtacggcacg aagtcccccg tcttcaggct gtcgatcctc acccaggggc cgagccgggt
      421 ccaggcaagc tcgcgctccg ggctctgggc tagccggccg tcggggccct gggcgcggac
      481 cgagagcttg tgcgtcccct cggtgaggcc ctcgggcgcg aggtcgaggt tgaagaagcc
      541 ctggggatcg ggcacggcgc ttcctgccgt cctgccgtcg aggaaggcct cgaccatggc
      601 ggcgccgccg gccacctcgg ccttcccgct caccctgagc gccccggtgc cggttgagcc
      661 cggcaggggg aagaggatct cgatcctatc ctcgggcagg gcgcccgtga ttcttatgtc
      721 acgggaggag aggctctcgt tgcccgcaag gtcccgcgcg atgagacgga ccgtatagag
      781 cccggggctc agggccgaga tgtccagttt cgtgcggacg atcctgcccg tgccgatgtc
      841 ggagctgagg acgctctgag tctgtttcgc cggcaggagc tcgatccggc atgaggcgag
      901 gccgaggttg tcggagacgc ggccgctcac gaggagctcg cgcgccgcct ccgagccgtc
      961 catgggcagg tcgagctggg tctcgggcgg ggtattgtcg accgtaagca gggcggcggt
     1021 gaagcccacc gtgtcgtagc cgtctaccgg gcgcagggcg atggccctgg ccccgtcggc
     1081 caggaggcgg gtgtcgaaac ggtaggacca ggccgagctg ccctcgggct tctggaagct
     1141 cgcccccgag tcgaaggaga gggccatggc cgagaccgag ttcgcgtcgg tggccgtccc
     1201 cgagagctcg acgatgcccg agagcacctt tgtcgagggc ggggagacga ggcgggcgaa
     1261 gggctcctcg gttgagatcc ggaacttcct ggaggttacc ttgccctcga tcccgtacac
     1321 gtcctcggcc ttggtctcga cgaggtgctc gttgtcgccg gtggcgagga tgccgatggg
     1381 cagcgagaag ctctggcccg agagctcgag gcgtttccac tccccaccgt cgatgcggta
     1441 ggagacggcc ttgaccccgt cgtcgtcgaa ggccaccccc gacatgacga agtcctggcg
     1501 caggacctcg ttctcgaggg gggcgtggat atcggccacg ggaaggtcgg cctccttgtc
     1561 gacggcgagg gcgggcgaga acacggcgct gttccccgcc ctgtcggtgg cgacgaagcc
     1621 cgcgccggcg ggcaggggga agtccagggc gttagcgtcg aggctggccc tgaaggtcga
     1681 gaggccctgg acctccactg cggggcccac ggccccggcg gccgcggcgc cttgagctgc
     1741 cgcggaaggc agggcggctg cggagccggc ggtggcggag ccggctgccg tcgcgggacc
     1801 ggcggccgtc gcgggaccgg gggccgccgc ggagccggcg gccgtcgcgg agccgggggc
     1861 cgtgaagacg aggccggcaa gcctgccatt gtcgctcgcc tcgcctatga aggtcaggag
     1921 gccgttcacg cgctccccgg gctcgggcag gacctgggtg atcaggggcg gggtcgcgtc
     1981 cttgaggaag agccggagct ccttggtctc cttgccggcg aggtccctcg ccaagatctc
     2041 gacgcgatgt gcgccatcgg cttggctcag ggccaggtcg gcctcgaagc ggtactcgcg
     2101 cgggtcaggc ggaggcggga ggacgggggc ggggggaggc ggcggggcct tggcgttggg
     2161 ctggggccag tagtcgtcgg ccttcctccg gctcccggtc ggcgccgccg gggcggcggg
     2221 ggccggggcc gcgaccgcgg cccgagacag ctcctggaac tcgcctccgt ctatcgagta
     2281 gcccacatag ccgaggccga gctcgtcgcg cgcgatgccc gacaccttaa gccggtcctt
     2341 gacccatatc gagtcggccg gcccggccag ctcgagcgag ggtgggcccg cgtcgacggt
     2401 gaagacagcc tggccccatt caaagacgtc gccgtctatg gtctggaccc tgaccgtcgt
     2461 cggcccggtc atcccctggg ccttggcctt gaggctgacg atcgggcctt cgaagctcgc
     2521 ctcgatgagc tcggtcgcgg gcttgagcga tacggagctg atgtccctgc ccttgaagcg
     2581 cccgacgagg gcctcgccgg gcgagaggag gacggccctc gccgccgatg ggtcgccggc
     2641 ggcgccgatg cgggcgtcct cgaagcggag agcgtctgcg tcgtcctcgc cgccggcggc
     2701 gggaaggatg gcgacgatga ggtatgagcg cgtgaccgag agcccggccg cgtccttggc
     2761 cgtgagcgat atgctcgccg ggccgtaggg aagctggggc agggctaggg taaagggaat
     2821 ggggccggag ggcgccggct ttccgagtgc gacgggaagc tgtcggtcgc cgatcctgca
     2881 ctcgatggag gagaggccgt tggccgcctc gacgctgccc gcaaggacgc tgccctggac
     2941 gatggcaagg ggaaggccag ggacccaggc agtcttggcc ttctggtcgc cgagctcgat
     3001 gctcccgatc gagggtccgc tcccgagggc gaggatctcc ctcttcgcct ccgtggtcct
     3061 gccacccgcg tcgcgggcga ggagggagac caggtgcttg cccgcgtcga gccggggaag
     3121 ggcgagggcg atggagcctc cctcgctcgt ggcgagctgt ttgccgtcta tggacaggac
     3181 gagggaagcc ggcccgtcgt cgtcgtccga cctgcaggag aggaggctgc caactcccag
     3241 atatttcccc gaatcgccca aggtaaggat ggccttgggc gcgtccgccg ccgcgtcata
     3301 gccgatcttg aaagccatgg tcgaggcgtt gcctacggcg tccctcgcgg tgaccgtggc
     3361 cacgatcccg ccggttttgg ccgaggccga ggggtcgagg atccgggcga aatagcgccc
     3421 gccctcggcg gcctccaggc tggtcttctc gccgcctagg ctcagctcgg ccgaggcgag
     3481 gccgcgcgcg tcctcgatcc tgaggcagag cgggatcggg cctggggcgt tcgaggacga
     3541 ggtctggggt gacaggaggc tgatggtcgg cgcgcccgtg tcgatgacta ggtagagggt
     3601 cgcgtagcct gagttcccgt cgaagtcctc ggtcttgagg atcagcctcg tctcgccctt
     3661 gagcttggca ggatcgaggc tgaaggagat gccgaggctg gcctgggccg tcttcctgtc
     3721 cttggccgtg gcgaggaccg cgtagctcgc gcccccgtcg aggctcaggg aaaccgtccg
     3781 cggcgccgag ccctccgggc ccgccatgct gcccttgaag ccgacggggc cggagaccag
     3841 gagcttgcgg gcgaagcgtt cggccgcctc ggccgctccc ggctcgggca ggctgggcag
     3901 gaggacggag agcttccggc cgccggtctg gcccgccagc tcgagccttc cctcgctggc
     3961 cgccggctcg acggacacct catccccggc gctgtcgggc ttgggggccg cgacgatcct
     4021 gaccgggggc cgcacgaggg cgctgccccc ggccctgtcg gtgaggcggt agacggcctg
     4081 ggggtgggcg gcgagatcgg cgcgatgcgc gaaatagcca tcgcggaaga ccaccggagt
     4141 gaaggccttc ccgtcctcgg agtactccac cgaggcgagg cccgagctgg agctcaccgt
     4201 ggccgcgatg tcctcgggcc cccagacctc ggcgtccctc tcgggagaca ggaagctggc
     4261 cttcggcggc tcgcccgcga tgcccagggc gaggaaggcc ctgccggtat tgcccgatgc
     4321 gtcggtggcc tcgacctcga tcgtggcgct gcccgtgctg cccgcgagat cggccgcgct
     4381 gacggcgaag gagtagcggc cgtcggcccc gcccttgatc tcggagctct tgccctccgg
     4441 caggatccgc cagctgagct tcgctatcga tcccggatcg ctcgcggtgc cgctcaccgt
     4501 gagcctgtcg cgcagccagg cgccctgggc cggcgagtcg atgccgattt ggggcggatc
     4561 gaggtcggtc acgaagacga agggcccggc cgagaactcg tgcccgagct gggtcttccc
     4621 gatgagcttc gtgggcgcgc tcctgccttc cttggccgcc gcgatgctca ccgccgctcc
     4681 cgtgaaggag gcctgcacga gctcggtggg
//
LOCUS       DASSQV010000225         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_678575, whole genome shotgun sequence.
ACCESSION   DASSQV010000225 DASSQV010000000
VERSION     DASSQV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678575"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFL04_03865"
     CDS             complement(<1..687)
                     /locus_tag="VFL04_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HET7838871.1"
                     /translation="MIHDRAARRRAGAAAGLATLIESRLRAARLFFRDIAGSGILALF
                     GFLGSLVLVAAVLVFIAEKAGGDSSFKSLFDGIWWAVVTVATVGYGDKYPVTVPGRLL
                     AIATILASIVLTSLISGTVASIFVERRIREGKGLQDLKLKNHLIICGWNPNAEAILRD
                     LEGIEGARGVSVVLVNWMEVEAFDAAKARFPALDLRFVRGDFTQEAVLRRASVKAASA
                     CVIVPDSSGDN"
     gene            865..1710
                     /locus_tag="VFL04_03870"
     CDS             865..1710
                     /locus_tag="VFL04_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HET7838872.1"
                     /translation="MPSHFEIDAEPSVVVADDSVIVVRKPGRMHTAPLPRPESRGPES
                     RESLCDWVFARFPDAATAVDAGRRRPAAEAGLIHRLDYETSGLVLFARTSASLSALLA
                     QQDSGMIAKEYLARTAPSEGFFPEGSRPLRWAPVGIEPSGWEAALREAGLGDAVPAAG
                     LLEVSRAKGALPSIESRFRPYGPGSRRVACLDPGLKAPAAATSESYLSWLVGSLAIGG
                     QLEFRVRLVSGFRHQVRAQLAWIGFPILGDPLYGGLESGRLHLHAARLEFRHPGSGES
                     LVLED"
     gene            1750..>3465
                     /locus_tag="VFL04_03875"
     CDS             1750..>3465
                     /locus_tag="VFL04_03875"
                     /inference="COORDINATES: protein motif:HMM:NF036553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HET7838873.1"
                     /translation="MPERSIFDTLVSELSAKERRDLLDQLARRSPVSKEPLFVDGSIP
                     GGEAQNYTERLESLGLLARFVLFLRGLFTGKSPEALLRADDLREIARRVEPRFPGLVD
                     TRHGYLLKGFADELKRLRDSARFFYDALDRSVEKDKSSFYAFLASMQIPEVHARLLEK
                     TEPARIEALRPSVTDSEMRSLCLDGFDEAFGELPEDGRRAMYHDVRSLLFLKRLSGFL
                     FERLLGCFRTGGSSLSVESAAVDEARDYLFDLGDILFSMASPPSASLMSALFLFVERD
                     ELAKPETDAARILGSDLEKAESALGKIRDFFNRIPFADILRLVSGDPAYSPRELAGGE
                     DWLATYKGFWRERVERQLEQWLIDRRMRELAAEVEAFLGKPGPVVLPHISREGRADSP
                     PVRQQQALSFLDGFFRGPFLRDLIRPLKILLVDGDFYRKDNQIEFTDAYDILFHLTET
                     IATFDSKLMPEGELGSAWSLVSAEIAPLKMKQRRTQNLVQTVNDEAERIIRRAGRALR
                     TMVEVIRGILKGEAGGKYDSLVNLSYLDGRGNKEFLRSLEKAKDKCERAYGLLSQLSG
                     LDLSQD"
BASE COUNT          551 a         1206 c         1147 g          561 t
ORIGIN      
        1 gttgtcgccc gagctgtcag ggacgatgac gcaggccgag gccgccttga ccgaggcccg
       61 cctgaggacg gcctcctgcg tgaagtcccc gcgcacgaac cgcaggtcga gggcggggaa
      121 gcgggccttg gcggcgtcga aggcctcgac ctccatccag ttcaccagga cgacggagac
      181 cccgcgcgcg ccctcgatgc cctcgaggtc gcggaggatg gcctcggcgt tggggttcca
      241 gccgcagatg atgaggtggt tcttcagctt caggtcctgc aagcccttac cctcccgtat
      301 ccttcgctcg acgaagatcg aggccaccgt accggagatc agcgaggtga gcacgatgct
      361 ggccaggatc gtggcgatgg cgagcagccg cccggggacg gtgacggggt acttgtcccc
      421 gtagcccacc gtggccacgg tgacgacggc ccaccagatc ccgtcgaaga ggctcttgaa
      481 actcgagtca ccgcccgcct tctcggcgat gaagacgagg acggctgcga caaggacaag
      541 gctgccgagg aaaccgaaca gggccaggat gccggaaccc gctatgtccc tgaaaaagag
      601 gcgggcagcc ctgagcctgg attcgatcag tgtggccaga cccgccgcgg cgcccgcgcg
      661 gcgcctggcc gccctgtcat ggatcatcct ggattccccc cgtttcggga agcggggtca
      721 gggagtcggc ccgaggcccc gcgaagccgt ctatccagta tcggcgcggc gcgcgcgggc
      781 cttgcgggtt ttcggcggag ggaagggggc cgcgcccgag cgcggagctt cacgcgcggg
      841 ggccggcgcg tatactatgt gccgatgccc agccatttcg agatcgacgc cgaaccgagc
      901 gtcgtggtcg cggacgacag cgtgatcgtg gtgaggaagc ccgggcgtat gcacactgcg
      961 cccctgcccc ggccggagag ccgggggccc gagagccgtg agagcctctg cgactgggtc
     1021 ttcgcgcgtt ttcccgacgc cgccacggcc gtggacgccg gaagacgcag accggccgcc
     1081 gaggccgggc tcatccaccg cctcgactac gagacctcgg gcctggtcct cttcgcccgc
     1141 acgagcgcct cgctctccgc cctcctcgca caacaggact cgggcatgat cgccaaggag
     1201 tatctcgccc gcaccgctcc ctccgagggc ttcttccccg agggctcccg gcccctgcgc
     1261 tgggcgcctg tggggatcga gccgagcgga tgggaggccg cgcttcgcga ggccgggctc
     1321 ggcgatgccg tgccagcggc cggccttcta gaggtctctc gggctaaggg tgccctgccc
     1381 tcgatcgagt ccaggttcag gccctacggc ccgggttccc gacgtgtcgc ctgcctcgat
     1441 cccgggctca aggccccggc cgcggccacg agcgagagct atctttcgtg gctcgtcggc
     1501 tccctggcca tcggcgggca gctcgagttc cgggtgcgcc tcgtctcggg cttccgccac
     1561 caggtgaggg cccagctcgc ctggatcggt tttcccattc tcggcgatcc cctgtatggg
     1621 ggactcgagt cggggcgtct gcatctgcac gccgcgaggc tcgaattcag acatccgggc
     1681 agcggggaaa gccttgtctt ggaggactga cgttgctttt tgcccctgct tgggctaggc
     1741 taagaaataa tgcctgagcg ctcgattttc gataccctcg tctccgagct ttcggcgaag
     1801 gagaggcgcg atctcctgga ccagctcgcc cggaggtcgc ccgtctccaa ggagccgctc
     1861 ttcgtcgacg ggtcgatccc cggcggagag gcgcagaact acacggagag gcttgagtcg
     1921 ctcgggctcc tcgccaggtt cgtcctcttc ctccgcggcc tcttcaccgg aaagagcccc
     1981 gaggccctgc tcagggccga cgacctcagg gagatcgcga ggagggtgga accccgtttc
     2041 cccggcctcg tcgacacgcg gcacggctac ctcctcaagg gcttcgccga cgagctcaag
     2101 cggctccggg attcggcgcg cttcttctat gacgccctcg acaggagcgt cgagaaggac
     2161 aagtcctcct tctacgcctt cctcgcctcg atgcagatcc ccgaggtgca cgcgaggctc
     2221 ctcgagaaga ccgagcccgc gaggatcgag gccctgaggc cgagcgtcac cgactcggag
     2281 atgcgcagcc tctgcctcga cggcttcgac gaggccttcg gcgagctgcc tgaggacggg
     2341 cggcgcgcga tgtaccacga cgtccgcagc ctccttttcc tgaagcggct ctcgggcttc
     2401 ctcttcgagc gcctcctcgg ctgcttccgc acggggggct cgagcctgag cgtcgagtcg
     2461 gccgccgtcg acgaggccag ggactacctc ttcgacctcg gcgacatcct cttctccatg
     2521 gcctccccgc cctcggcctc cctcatgtcg gccctcttcc ttttcgtcga gcgcgacgag
     2581 ctcgccaaac ccgagaccga cgccgcgagg atcctgggct cggacctcga gaaggccgag
     2641 agcgccctgg gcaagatcag ggatttcttc aaccggatac ccttcgccga catcctgcgc
     2701 ctcgtctccg gggatccggc ctattccccg agggagctcg cggggggcga ggattggctg
     2761 gcgacctaca agggcttctg gcgcgaacgc gtggagcgcc agctcgagca atggctcatc
     2821 gaccgcagga tgcgggagct ggccgccgag gtcgaggcct tcctgggcaa gccgggtccc
     2881 gtcgtcctgc cccacatctc ccgcgaaggc agggccgaca gcccgccggt gcggcagcag
     2941 caggccctga gcttcctcga cggcttcttc cgtggaccct tcctgaggga cctcatcagg
     3001 ccgctcaaga tcctcctcgt cgacggcgac ttctaccgca aggacaacca gatcgagttc
     3061 accgacgcct acgacatcct gttccacctc accgagacca tcgccacctt cgactccaag
     3121 ctcatgcccg aaggggagct gggctcggcc tggtccctcg tcagcgccga gatagccccc
     3181 ttgaagatga agcagaggag gacccagaac ctcgtgcaga cggtcaacga cgaggccgag
     3241 cggatcatca ggagggcggg ccgggccctc aggacgatgg tggaggtgat ccgcgggatc
     3301 ctcaagggag aggcgggggg gaagtacgat tccctcgtca acctctccta cctcgacggg
     3361 aggggcaaca aggaattcct gcggagcctc gagaaggcca aggacaagtg cgagcgggcc
     3421 tatggcctcc tctcccagct ctccggcctc gacctgagcc aggac
//
LOCUS       DASSQV010000226         2955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1672412, whole genome shotgun sequence.
ACCESSION   DASSQV010000226 DASSQV010000000
VERSION     DASSQV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672412"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..750)
                     /locus_tag="VFL04_03880"
     CDS             complement(70..750)
                     /locus_tag="VFL04_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838874.1"
                     /translation="MRTAPLLLLAAGLAVLGAAARAQESGERDYFSAVFWIDLEPISR
                     AGEPFPLSPQEGAARLLDEAAWVYAGMLFGWDFAYTPLDRTRAIEERFELKALGSIPR
                     GDRRIVPGPASGDSNELRSRVDFLPEPQDLALIQSYSHAPWRPAQGLGSADYMLGQAG
                     RRRAYEDGLREAVRTLLRLTEYNKPRLVRGRVALDRVPTIAISGGRYVVQLRARVEVS
                     EILSYNAY"
     gene            complement(767..1408)
                     /locus_tag="VFL04_03885"
     CDS             complement(767..1408)
                     /locus_tag="VFL04_03885"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7838875.1"
                     /translation="MDRNSIIRRPFSYSNRNAALWLIAANVFVFALEYLFPRLIGLLV
                     MTPVSVLGGAFWQPFTYMFVHDPRGFTHILVNMLGLFFFGTQVERELGTREFLLYYLL
                     TGLLAGVFSFLTYLFFGGAYVSLLGASGAVFALLLAFATLFPQAEIYIWGILPIRAPL
                     MVVGYAALEIVSQVFSFQSSVAHLTHIAGFGFGWLYFLARFGVNPMRRMFPRR"
     gene            1472..1660
                     /locus_tag="VFL04_03890"
     CDS             1472..1660
                     /locus_tag="VFL04_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838876.1"
                     /translation="METAEGKAPNCLKCVHFRITWEPAFPRSCLVFGIKSRNLPSVEV
                     FAATGRHCPSFELKAGLK"
     gene            1716..2633
                     /gene="glsA"
                     /locus_tag="VFL04_03895"
     CDS             1716..2633
                     /gene="glsA"
                     /locus_tag="VFL04_03895"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HET7838877.1"
                     /translation="MRELLDTLVAEGRAAAARGRCAQYIPALSRADPRAVGIAVTSAD
                     GGLERAGDSSTRFTVQSVSKAMALAYVMGAGGEDKVFSRVGKEPSGDPFNSIIRLETS
                     ERGRPFNPMINAGAIVVSGLMPGITGREKVEGLASFVADVLGREDLGIDEEVYASEEA
                     TANRNRSIAWFLKELGLIDGQVDETLDAYFRQCALLLDAADLSRFAAMLALDGLTAGS
                     GRRVISERDARIVKSLMLTCGLYDGSGEFCAEAGLPAKSGVGGGIMASARGRLGIGSF
                     GPALDERGNSIAGQLMIARLSKELGLSAL"
     gene            complement(2674..2745)
                     /locus_tag="VFL04_03900"
     tRNA            complement(2674..2745)
                     /locus_tag="VFL04_03900"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2711..2713),aa:Cys,seq:gca)
BASE COUNT          497 a          963 c         1047 g          448 t
ORIGIN      
        1 cggccccata ggagccaggg ccgggtgtgc ggctgagccg cggcccggcc accgcacccc
       61 cctccgcgct cagtacgcgt tgtacgacag tatctccgag acctcgaccc tcgcccgaag
      121 ctggaccacg tagcgcccgc cgctgatggc tatcgtgggc acccggtcga gggccaccct
      181 cccccggacc aggcggggct tgttgtactc ggtgagtcgg agcagggtcc tcaccgcctc
      241 acggagcccg tcctcgtagg ccctccgcct tcctgcctgc cccagcatgt agtcggcgct
      301 cccgaggccc tgggcgggcc tccagggggc gtgggagtag gactggatga gggccaggtc
      361 ctggggctcg gggaggaagt cgacgcggga gcggagctcg ttcgagtcgc ccgaggccgg
      421 accgggcacg atccgcctgt cgcccctcgg gatcgagccg agggccttga gctcgaagcg
      481 ctcctcgatc gccctcgtcc tgtccagggg cgtgtaggcg aagtcccagc cgaagagcat
      541 gcccgcgtag acccatgcgg cctcgtcaag gagcctcgcg gccccctcct gggggctcag
      601 gggaaagggc tcgccggccc tggagatggg ctcgaggtcg atccagaaca ccgcgctgaa
      661 gtagtcgcgc tcccccgatt cctgggcccg cgcagccgcg cccaggaccg cgaggccggc
      721 ggccaggagg aggagcggcg cggtccgcat cccggtgggc ccgccctcag cgccgcggga
      781 acatgcggcg catcgggttc acgccgaagc gcgcgaggaa gtagagccag ccgaagccga
      841 agccggctat gtgggtgaga tgcgcgacgc tgctctggaa gctgaagacc tgggagacga
      901 tctcaagggc cgcgtagccg acgaccatga ggggggccct gatcggcagg atgccccaga
      961 tgtagatctc ggcctgcggg aaaagggtgg cgaaggcgag gaggagggcg aagaccgcgc
     1021 ccgaggcccc gaggagggag acgtaggccc cgccgaagaa gaggtaggtg aggaaggaga
     1081 agaccccggc gaggaggccc gtgaggaggt agtagagcag gaactccctc gtgccgagct
     1141 cgcgctcgac ctgggtgccg aagaagaaga ggcccagcat gttcacgagg atgtgggtga
     1201 agcccctggg gtcgtggacg aacatgtagg tgaagggctg ccagaaggcg ccgccgagca
     1261 cgctcacggg tgtcatgacg aggaggccga tgagccgcgg gaagaggtac tcgagcgcga
     1321 agacaaacac gtttgccgcg atgagccaga gcgccgcgtt cctgttcgag tagctgaagg
     1381 gcctgcggat gatggagttc ctgtccatct gattactcta aggaattccg ggctcgacgg
     1441 caagcacggc ccgggtatac aatccgccgc gatggaaacc gcggagggca aggcgccgaa
     1501 ctgcctcaag tgcgtccact tccgcatcac ctgggagccg gccttccccc gctcctgcct
     1561 cgtcttcggc atcaagagcc gcaacctgcc ctcggtcgag gtcttcgccg cgacggggcg
     1621 gcactgtccg tccttcgagc tgaaggccgg gctcaagtag cggaggctga gggccaccca
     1681 tttgccgcgc gggccgcgcc cgctatactc gggccatgcg tgagctcctc gataccctcg
     1741 tcgccgaagg ccgggccgcg gccgcgcgcg gccgttgcgc gcagtacatc cccgcccttt
     1801 ccagggccga tccccgggcg gtcggcatcg cggtcacgag cgccgacggc ggcctcgaac
     1861 gcgctggcga ctcctccacg cgcttcaccg tccagagcgt gtccaaggcg atggccctcg
     1921 cctacgtcat gggggccggg ggcgaggaca aggtgttctc gcgggtgggc aaggagccga
     1981 gcggcgaccc cttcaactcg atcatccgcc tcgagacctc ggagcgcggc aggcccttca
     2041 accccatgat caacgcgggc gccatcgtcg tgagcggcct catgcccggg atcacgggcc
     2101 gcgagaaggt cgagggcctc gcgtccttcg tcgccgacgt gctcggtcgc gaggacctcg
     2161 gcatcgacga ggaggtctac gcctcggagg aggcgacggc caaccgcaac cgctcgatcg
     2221 cctggttcct caaggagctc ggcctcatcg atggccaggt cgacgagacc ctggacgcct
     2281 acttccgcca gtgcgccctg ctgctcgacg cggccgacct ctcgcgcttc gccgcgatgc
     2341 tcgcgctcga cgggctgacg gccggctcgg gccggcgcgt catctcggag cgcgacgcga
     2401 ggatcgtgaa gagcctcatg ctcacctgcg ggctctatga cggctcgggc gagttctgcg
     2461 ccgaggcggg gctccccgcg aaaagcggcg tgggcggcgg catcatggcc tcggcccggg
     2521 gcaggctcgg catcgggagt ttcgggccgg ccctggacga gaggggcaac tcgatcgccg
     2581 gccagctgat gatcgccagg ctgtcaaagg agcttggcct gtcagctctc taggcaaaaa
     2641 aaaccggaat ggcgatgcca ttccggtttt tcgaggcgcc ggtcggagtc ggaccgactc
     2701 atgaaggttt tgcagacctc tcccttccca cttgggtacg gcgccgtttt atgacactca
     2761 tcgcgtctga gtgaatcgga cgcattgggt gattgcgagt gcaaaatata tacaacgacg
     2821 gattctttgg caagtgtttg ggttcggatc gggggggaaa gaaaaaaggc gcgagcattg
     2881 agctcgcgcc ttccttccag cagaagaccc gcgctatttc ttgccgggct ttgcggtcgc
     2941 gaggttcgtc gcggc
//
LOCUS       DASSQV010000227         3855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_305236, whole genome shotgun sequence.
ACCESSION   DASSQV010000227 DASSQV010000000
VERSION     DASSQV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305236"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFL04_03905"
     CDS             <1..556
                     /locus_tag="VFL04_03905"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET7838878.1"
                     /translation="SVLRGLDRLFGTALGGELLSRLGAGIGSDVPFFFTEGAALVSGR
                     GEHVEAVRPRRDLALALVFPGFPVSTAEAYGLLDRSRPDDSSEEDLGPEAIEAAYRSD
                     PRTWTFANSFEPWVGAAHPRIPALRDSLLRLGASFAAMSGSGSTVFGVFSDPEACELA
                     CRRLGEEGERARSVLPLARLPELH"
     gene            599..883
                     /gene="spoVG"
                     /locus_tag="VFL04_03910"
     CDS             599..883
                     /gene="spoVG"
                     /locus_tag="VFL04_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HET7838879.1"
                     /translation="MEITDIRIRRVTTEGKLKAYVTVTFDDCFVVHNVKIIEGKSGVF
                     IAMPSRKTRNGEYKDIAHPIHPDFRTRLQDRILEAFETAPMTDGSAIDMD"
     gene            914..985
                     /locus_tag="VFL04_03915"
     tRNA            914..985
                     /locus_tag="VFL04_03915"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:946..948,aa:Gln,seq:ttg)
     gene            1023..1715
                     /locus_tag="VFL04_03920"
     CDS             1023..1715
                     /locus_tag="VFL04_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET7838880.1"
                     /translation="MNNPVLNAVARVETTKGELNQLRKAGKIPAVVYGGGQAAVHILL
                     DEKEFLKATAGISESTIISMDIDGKKSRAFVKARQRNTLTNRIIHIDFLEVVSGRMLT
                     AKVPVHLVGAPVGVREGGILENPAHEIMVECDPDHLPEKIDIDVSELHANHSIHVRDI
                     PAMDKVKILSSSDLVVAVVKYAKAEAVATPVAEAAAAPGAAPAAGAPGAAPAPAAAGA
                     AAPAAEKKAEKK"
     gene            1785..3170
                     /gene="tilS"
                     /locus_tag="VFL04_03925"
     CDS             1785..3170
                     /gene="tilS"
                     /locus_tag="VFL04_03925"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET7838881.1"
                     /translation="MKYARAKGRVEACLLEFLVSHSVPAEERLCVAYSGGPDSTALLA
                     AAAAVCPRPPLAAHVDHGLRPEAEIEAELSLVRASCSNLGAGLRIAHLGRGSVERLAR
                     ESGAGVEAAARELRYRALGGLMRRAGLHRILFAHTRDDLAEGLLMRLLSGSGSGGLRG
                     IPPMRGAVLRPFLGLGKADLLGYLGERGLAFSTDSSNASMGFLRNRVRSRLVPLLDQD
                     FPGWRRGLAGTARRAGEEAEALEAAALALCPAFVPSLGGGLEADERAFGSAPLALRLR
                     SLVDALGRVAGSSRASMPMARAALRALEGGATVYRGGGIEVSLAEGRIRVAPEPGYGL
                     DFPRIDGYFVVVDRPRRIRVGALIVEASWTTGAGSGIRADAFRFPLVIRSRRPGDAIA
                     IPGGEKRLDELFSDWGLPPRLRGVVPIAEDRHGIVAVLGAAFGAKDRYRTGSGPEGPG
                     RRRLSVVVKGA"
     gene            3178..>3855
                     /locus_tag="VFL04_03930"
     CDS             3178..>3855
                     /locus_tag="VFL04_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HET7838882.1"
                     /translation="MDFDDQNQKPPRKTGPEGPARGSRAALALFLSLGVLFSALFLFN
                     ERNDATEIPYSAFLTYLDQGNVDAVKIVDQFEIQGTLKGKTGAQALFKTNIPYVDLEL
                     LKQLRDKQVKITGSVKGTSPLQILAEFAPWIFGFFIIWFMLRQMQGNNKASTFGKSKA
                     KRYIEPAKKITFADVAGQKEAKYELLEVVDYLKNPGKFVKMGARIPKGVLLVGMPGTG
                     KTLLAKAV"
BASE COUNT          642 a         1354 c         1251 g          608 t
ORIGIN      
        1 ctcggtcctc cgcggcctcg atcggctctt cgggaccgcc ctcggcgggg agctgctttc
       61 ccgcctcggg gccggcatcg ggagcgatgt gcccttcttc ttcacggagg gggcggccct
      121 cgtctcgggc aggggggagc acgtcgaggc ggtacggccg aggcgggacc tggccctggc
      181 cctcgtgttc cccggtttcc cggtcagcac ggccgaggcc tacggcctcc tcgaccgctc
      241 gcggccagac gattcctccg aggaggactt gggcccggaa gccatcgagg cggcctaccg
      301 ctccgatccc cgcacctgga ccttcgccaa ctccttcgag ccctgggtcg gcgcggccca
      361 tccgaggata ccggcccttc gcgactcgct tctcaggctg ggagccagct tcgccgccat
      421 gtcaggatcg ggcagcacgg tcttcggggt cttcagcgac ccggaggcct gcgagctggc
      481 ctgcaggcgg ctgggtgagg agggcgagcg ggcccgttcc gtgctgccgc ttgcgcggct
      541 tccggagctt cactagaatt gtggcagaag caaaagtgcc gcaaggagtg tagcagccat
      601 ggagatcacg gatatccgga tccggcgggt cacgaccgag gggaagctca aggcatacgt
      661 gacggtgacc ttcgacgact gcttcgtcgt tcacaatgtc aagatcatcg agggcaagag
      721 cggagtcttc atagcgatgc cgagccggaa gacgaggaac ggagagtaca aggacatcgc
      781 ccacccgata catcccgatt tccggaccag actgcaggac cgcatcctcg aggccttcga
      841 gacggctccc atgacggacg gttccgcgat cgacatggac tgacaggacc gacggtttct
      901 ctcgcctgct ctttgggacg tcggcaagcg gtaagcctcc ggattttggt tccggcattc
      961 gcaggttcga atcctgccgt cccagctaac cactcaaatt catggtcaag gagactatac
     1021 agatgaataa tccagtgctg aacgccgtcg cccgcgtgga gacgacgaaa ggcgagctca
     1081 accagcttcg caaggcgggc aagatcccgg ccgtcgtcta cggcggcggc caggccgccg
     1141 tccacatcct cctcgacgag aaggagttcc tcaaggccac cgccggcatc tccgagtcga
     1201 ccatcatcag catggacatc gacggcaaga agtccagggc cttcgtgaag gcgcgccagc
     1261 gcaacaccct gacgaacagg atcatccaca tcgacttcct cgaggtcgtc tccggcagga
     1321 tgctcaccgc gaaagtcccc gtgcacctcg tcggcgcccc cgtcggcgtc cgcgagggcg
     1381 gcatcctcga gaacccggct cacgagatca tggtcgagtg cgatcccgac cacctccccg
     1441 agaagatcga catcgacgta tccgagctcc acgcgaacca ctcgatccac gtgcgcgaca
     1501 ttcccgccat ggacaaggtg aagatcctct ccagctccga cctcgtcgtg gccgtggtca
     1561 agtacgcgaa ggccgaggcc gtcgcgaccc ccgtcgccga agccgccgcc gcccctggcg
     1621 ccgcccccgc cgccggagcc cccggcgctg cgcctgcccc cgccgcggcc ggcgcagctg
     1681 cccctgccgc cgagaagaag gccgagaaga agtagccgaa gtccacggaa tgagactcga
     1741 gaggggaggg caacctcccc ttttcgcatt ggagcgggtg caggatgaag tacgctcgcg
     1801 cgaagggccg ggtggaggct tgcctcctgg agttcctcgt ctcccattcg gttcccgccg
     1861 aggagaggct ctgcgtcgcc tactcgggag ggccggactc gaccgccctg ctcgcggcgg
     1921 ccgccgccgt ctgcccgagg cctcccctcg ccgcccatgt cgaccatggc ctcaggcccg
     1981 aggccgagat cgaggccgag ctctcgctcg tccgcgcgag ctgctccaac cttggggccg
     2041 ggctcaggat cgcccatctc ggccgcggct cggtggagag gctagccagg gaatccggtg
     2101 cgggcgtcga ggccgccgcc agggagctcc gctaccgcgc cctcggcggc ctcatgcgga
     2161 gggccgggct ccacaggatc ctgttcgccc acacccgcga cgaccttgcc gagggcctcc
     2221 tgatgcggct cctcagcggc tcgggttcgg gcgggctccg cgggatcccc cccatgaggg
     2281 gcgccgtcct gcgccccttc ctcggcctcg gcaaggccga tctcctcggc tatctcgggg
     2341 agcgggggct ggccttcagc acggattcga gcaacgcgtc catgggcttc ctccgcaaca
     2401 gggtgcgcag ccgcctcgtc cccctgctcg accaggactt cccgggctgg cgccggggac
     2461 tcgcgggcac ggcgcggagg gcgggcgagg aggctgaggc ccttgaggcc gcggccctgg
     2521 ccctctgccc ggccttcgtc ccctccctcg ggggcggcct tgaggccgat gagcgggcct
     2581 tcggctccgc ccccctggcc ctcaggctcc gctccctcgt cgatgccctc gggcgggtgg
     2641 ccggctcctc aagggccagc atgcccatgg cccgcgcggc cctcagggcc ctcgagggcg
     2701 gggccaccgt ctaccggggc ggcggcatcg aggtctcgct ggccgaaggc cgcatccgcg
     2761 tcgcgcccga gcccggctat ggccttgatt ttcctcgtat cgacggatat tttgtagtag
     2821 tcgatcggcc tcgccggata agagtaggcg cgctcatcgt ggaggccagc tggaccacgg
     2881 gagcgggctc tggcattcgc gcggacgcat tccgattccc ccttgtcatc cgttctcgac
     2941 gccctggcga cgcgatagcg attccgggcg gcgaaaagcg ccttgacgag ctgttctccg
     3001 actgggggct cccaccgcgg ctgcgcgggg tggtccccat cgccgaggac agacacggca
     3061 tagtcgcggt actgggcgcg gccttcggtg cgaaggaccg ctacaggacc gggtcagggc
     3121 cggaggggcc ggggcgccgg aggctctcgg tcgtcgtgaa aggagcctga gccctatatg
     3181 gatttcgacg accagaacca aaaacccccg aggaagacgg gacccgaagg cccggcccgc
     3241 ggaagcaggg ccgcgctcgc gctcttcctc tccctcggcg tcctcttctc agccctcttc
     3301 ctcttcaacg agaggaacga cgcgaccgaa atcccctact cggccttcct tacctacctc
     3361 gaccagggca acgtggacgc ggtcaagatc gtcgaccagt tcgagatcca gggcacgctc
     3421 aagggcaaga cgggggccca ggccctgttc aagaccaaca taccctatgt cgacctcgag
     3481 ctcctcaagc agctgcgcga caaacaggtg aagatcaccg gatcggtgaa gggcacgagc
     3541 cccctgcaga tactcgccga gtttgcgccg tggatattcg gcttcttcat catctggttc
     3601 atgctcaggc agatgcaggg caacaacaag gcctccacct tcggcaagag caaggccaag
     3661 cgctacatcg agccggccaa gaagatcacc ttcgccgacg tcgccggcca gaaggaggcc
     3721 aagtacgagc tcctcgaggt ggtcgattac ctcaagaacc ccgggaagtt cgtcaagatg
     3781 ggggcgagga tacccaaggg cgtcctcctc gtgggcatgc ccggcacggg caagaccctc
     3841 ctcgccaaag ccgtc
//
LOCUS       DASSQV010000228         3037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2171233, whole genome shotgun sequence.
ACCESSION   DASSQV010000228 DASSQV010000000
VERSION     DASSQV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2171233"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VFL04_03935"
     CDS             <1..944
                     /locus_tag="VFL04_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET7838883.1"
                     /translation="STTATFSVRAASSRIDLVRAAFVLALLEGASAILVAAPGSRQVG
                     PVLVAGLLGAANGFACALLTLALLPLLEQALNAPTRFRLMELSDLNAPILKRLFNVAP
                     GTYSHSVSVAHLAESACREIGADPLLARVGAYYHDIGKIEQPEYFIENQSGYNKHEEI
                     NPRLSATVIRSHVKIGAEKARALGLPQAVVDIIAQHHGNGIIAWFYEQAKKAEGGADA
                     DDFSYPGQPPAGREAAVVMLADAVEAASRTLKRPTMTRLEDFIREIIMDKVKQGQLDR
                     CDLTFKDLDAIRTSFTRILAGHFHSRIEYPKLKEQPR"
     gene            941..1420
                     /gene="ybeY"
                     /locus_tag="VFL04_03940"
     CDS             941..1420
                     /gene="ybeY"
                     /locus_tag="VFL04_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET7838884.1"
                     /translation="MNEVEVSVEGIGTPPWLERARSYALAVLARLGKDNWELSLLLCD
                     DSFIRGLNRQYRDRDEATDVLSFEQGDSFTTPEGESRFLAGDIVVSLEALKRNAEDFG
                     ISQDEELRRLIVHGILHLTGMDHEDLDPSKPMLALQEELLRELDAASGGPGRGGAIL"
     gene            1420..2247
                     /locus_tag="VFL04_03945"
     CDS             1420..2247
                     /locus_tag="VFL04_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET7838885.1"
                     /translation="MSLISRILGRRQGEPGPARAGEDSGAAERREMIKGVQELALTTV
                     KEVMVPRIDTVFLPLGIGREELLEKVVSCGHSRIPVYRETIDEVVGVLYAKDLLHAMV
                     RGGELSLAELARKPYFVPESKRIDSLLREFRRRRVHIAVAVDEYGGVSGIVCLEDIIE
                     EIVGDIQDEFDEEGEDVVALGDGTWRVDARADLDDLNEELDLGLPADRFDTLGGYVFD
                     LFGRIPGRNELVSAGGLDFVILDMDGHRITSVKIARRAADDLEGADHVGEEPAAEKE"
     gene            2253..>3037
                     /locus_tag="VFL04_03950"
     CDS             2253..>3037
                     /locus_tag="VFL04_03950"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7838886.1"
                     /translation="MGPRHTRQLQPRLLACLLVAAALAVGLAPPGLSAQAGLSAQAGV
                     PAQAPQADARSVFAEAERLRFAEDWYGAIEAYLSVLQKNPSYGEAIYALAECYYELEE
                     YDQALAYARRAAPFMRGESALSDLEGFIRVGSGDLAGARGVFEGVLANRPNELDARFG
                     MALLDLAAGRKTEARVRLEDSLRISPQNGRALLSLALMASDQGRDADARALIEKALRF
                     HGSEPRTQYVAASLAADDGDIEGATFHARNALDLKPDYGEARLL"
BASE COUNT          476 a         1171 c          953 g          437 t
ORIGIN      
        1 cctcgaccac agccaccttc tcggtccgcg cggcaagctc ccgcatcgac ctcgtccggg
       61 cggccttcgt cctcgccctc cttgaggggg catcggccat cctcgtcgcg gccccgggct
      121 cccgccaggt agggcctgtc ctcgtcgccg gcctcctggg agcggccaac ggcttcgcct
      181 gcgccctcct caccctggcc ctcctgcccc tcctcgagca ggccctcaac gcgcccaccc
      241 gcttcaggct catggagctc tccgacctca acgcccccat cctcaagcgg ctcttcaacg
      301 tggccccggg cacctacagc cactcggtct ccgtggccca cctcgccgag tcggcctgcc
      361 gcgagatcgg tgccgacccc ctgctcgccc gggtcggggc ctactaccac gacatcggga
      421 agatcgagca gcccgagtac ttcatcgaga accagtcggg ctacaacaag cacgaggaga
      481 tcaacccgag gctctcggcc acggtcatac ggagccacgt caagatcggg gccgagaagg
      541 cgagggccct tggcctgccc caggccgtcg tcgacatcat cgcccagcac cacggcaacg
      601 ggatcatcgc ctggttctac gagcaggcca agaaggccga gggcggggcc gacgccgacg
      661 acttctccta ccccggccag ccccccgccg gccgcgaggc ggccgtggtg atgctcgccg
      721 acgccgtcga ggccgcttca cggaccctca agcgccccac gatgaccagg ctcgaggact
      781 tcatccgcga gatcatcatg gacaaggtga agcagggcca gctcgaccgc tgcgacctca
      841 ccttcaagga cctcgacgcc atccgcacga gcttcacccg gatcctcgcc gggcatttcc
      901 actcgaggat cgagtacccg aagctcaagg agcagccgag atgaacgagg tcgaggtatc
      961 ggtcgagggc atcggcacgc cgccttggct cgagcgggcc cgctcctacg ccctggccgt
     1021 cctggccagg ctcggcaagg ataactggga gctctccctc ctcctctgcg acgactcctt
     1081 catccggggc ctcaaccggc agtaccgcga ccgcgacgag gccaccgacg tcctctcctt
     1141 cgagcagggc gacagcttca ccactcccga gggcgagagc cgcttcctcg cgggcgacat
     1201 cgtcgtgtcg ctcgaggccc tcaagcggaa cgccgaggac ttcggcatct cccaggacga
     1261 ggagctccgc cgcctcatcg tccacggcat cctccacctc acgggcatgg accacgagga
     1321 cctcgacccg tccaagccca tgctcgccct ccaggaggag ctcctccgcg agctcgacgc
     1381 ggcctcgggg ggcccgggac ggggaggggc catcctgtga tgagcctcat ctccaggatc
     1441 ctcggccgca gacagggcga gcccggcccc gccagggccg gggaggacag cggggcggcc
     1501 gagcgccgcg agatgatcaa gggcgtgcag gagctcgccc tcaccacggt caaggaggtc
     1561 atggtccccc gcatcgacac cgtcttcctc cccctcggca tcgggcgcga ggagctcctg
     1621 gagaaggtcg tcagctgcgg acactcccgg atccccgtgt accgcgagac catcgacgag
     1681 gtcgtcggcg tgctctacgc caaggacctc ctccacgcca tggtgagggg cggcgagctc
     1741 tccctcgccg agctggcccg caagccctac ttcgtccccg aatccaaacg catcgattcg
     1801 ctcctcaggg agttccgccg ccgccgcgtc cacatcgccg tggccgtcga cgagtacggc
     1861 ggggtctcgg gcatcgtctg tctcgaggac atcatcgagg agatcgtcgg cgacatccag
     1921 gacgagttcg acgaggaggg cgaggacgtc gtggccctgg gcgacgggac ctggcgggtc
     1981 gacgcccgcg ccgacctcga cgacctcaac gaggagctcg acctcggcct gcccgccgac
     2041 cgcttcgaca ccctcggggg ctatgtcttc gacctcttcg gcaggatacc gggcaggaac
     2101 gagctggtga gcgctggcgg tctcgacttc gtgatcctcg acatggacgg gcaccgaatc
     2161 acctccgtga agatagcccg ccgagctgcc gatgatctgg agggagccga ccatgtgggc
     2221 gaagagcccg cggcggagaa ggagtaaagc ccgtgggccc tcgccatacc cgacagctgc
     2281 agcctcgtct ccttgcctgc ctccttgtgg cggctgccct cgccgtcggc ctggcccctc
     2341 ctgggctttc ggcccaggcc gggctctcgg cccaggccgg ggttcctgcc caagcgcccc
     2401 aggccgacgc gcgctccgtc ttcgccgagg ccgagcggct ccgcttcgcc gaggactggt
     2461 acggggccat cgaggcctac ctctccgtcc tccagaagaa cccctcctac ggcgaggcga
     2521 tctatgccct cgccgagtgc tactacgagc tcgaggagta cgaccaggcc ctggcctacg
     2581 ccaggcgggc ggcccccttc atgcgcggcg agagcgccct ctccgacctc gagggcttca
     2641 tccgggtcgg ctcgggcgac ctcgcgggcg cccgcggggt cttcgagggg gtgctcgcca
     2701 accgcccgaa tgagctggac gcgcgtttcg gcatggccct cctcgacctg gcggcggggc
     2761 gcaagaccga ggcgagggtg aggctcgagg acagcctccg catctcgccc cagaacggcc
     2821 gcgccctcct ctccctggcc ctcatggcct ccgaccaggg ccgcgacgcc gacgcccgcg
     2881 ccctcatcga gaaggccctc cgtttccacg gcagcgagcc ccgcacccag tacgtcgccg
     2941 cctcgctcgc ggccgatgac ggggacatcg agggcgccac cttccacgcg cggaacgccc
     3001 tcgacctcaa acccgactac ggcgaggccc gcctcct
//
LOCUS       DASSQV010000229         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2046765, whole genome shotgun sequence.
ACCESSION   DASSQV010000229 DASSQV010000000
VERSION     DASSQV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046765"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VFL04_03955"
     CDS             complement(<1..697)
                     /locus_tag="VFL04_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7838887.1"
                     /translation="MGRYIAKRLLSLIPVLFGVSVIVFFLVRLIPGSALQMYMGTQVE
                     ASPAQMEELKRIFGEDRPLPVLYVEWLGRLLQGDFGVSLRTGRPVLPDILSRFPLSLE
                     LTLMALVLALAIGIPQGILASLRRNPVLDLANRVVGLLGLSLPQFWLAALMVLGFSGF
                     RGWIPMGNFVSFAASPLKNLQMLLLPSLAIGIGLAAVIMRYTRNSMLEVLQMDYIRTA
                     RAKGLKRSVVIFRH"
     gene            complement(788..2308)
                     /locus_tag="VFL04_03960"
     CDS             complement(788..2308)
                     /locus_tag="VFL04_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7838888.1"
                     /translation="MKRLMILLVALAVAALIPTAGAFAAPAEKVITIGVDQEAVGLDP
                     HIVTAFSSHRRLDLLYNRLVRIDDSMAIVPDLAESWDNPNNTTYVFHLRKGVKFHNGR
                     EMTSDDVVFSLNRVLDPKTASPGRSYIATVTAIEAVDKYTVKLTLSGPLPSLLDGLSS
                     NNLAIVAKEAVAANGNLQKVEAGTGPFMLKEWVADNSMTLVRNPDYFEKGAPTADKVI
                     FRVIPEQASLLAGVKSGELDMATINDGAIIRQAAKDPKVTVMSKPGLNMRIFSFNTTR
                     KPFDNVLVRQAVSLALDAPEILTIAEFGMGKVTGPLPIAATKWAAAPSTLPFTGPDLA
                     QAKSLLAKAGFPNGFTMKITCSSTYEGGLAVAQVAQDELKKIGITAELEVVEWGVYID
                     KWVKRDFDTMIELRGGSAEPDRFLYRTFHSTGGVNNFLFKDAAVDGLLDAGRGQTAYA
                     DRKVTYDKLQYLLSEKAPAVFLYCPNENQVLSKSLSGFKLVGNGSLYYLTNTDVKR"
     gene            complement(2343..>2691)
                     /locus_tag="VFL04_03965"
     CDS             complement(2343..>2691)
                     /locus_tag="VFL04_03965"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HET7838889.1"
                     /translation="RGRGLSYADTVATATAFTAESIAAAYRDFILPRTPIAAVLVAGG
                     GALNPCLLGMIQERLPAGIRVTTTAAHGVPDQAREAMAFAVLGHESLVGRPGNLPAVT
                     GARSPVVLGSITL"
BASE COUNT          431 a          793 c         1023 g          444 t
ORIGIN      
        1 cgtggcggaa gataaccacg ctgcgcttga ggcccttggc cctcgcggtc ctgatgtagt
       61 ccatctggag gacctcgagc atgctgttgc gcgtgtagcg catgatgacg gccgcgagcc
      121 cgatccctat ggccagggag ggcaggagga gcatctggag gttcttgagg gggctggccg
      181 cgaagctcac gaagttgccc atggggatcc agccccggaa gccggagaag cccaggacca
      241 tgagggcggc gagccagaac tggggcaggg agagcccgag gaggcccacc acgcggttgg
      301 cgaggtccag gacggggttc cgcctgagcg aggcgaggat gccctggggg atgccgatgg
      361 cgagggcgag gacgagggcc atgagggtga gctcgaggct cagggggaag cgggagagga
      421 tgtccgggag cacgggccgc cccgtgcgca gcgagacgcc gaagtcgcct tggaggagac
      481 ggccgagcca ctcgacatag aggaccggca ggggcctgtc ctcgccgaag atccgcttga
      541 gctcctccat ctgggccggg ctcgcctcga cctgggtgcc catgtacatc tggagggccg
      601 agcccggtat gaggcggacg aggaagaaca cgatgaccga caccccgaag agcacgggta
      661 tcagcgagag gagccgcttg gcgatgtatc tgcccacgtc ctactccgga atgagagaaa
      721 tgcaaccacg gagacacgga gtgccctccg cgtcctccgt ggtcagaatc gtcctgggcc
      781 gcgcagccta gcgcttcacg tccgtgttgg tcaggtagta caggctgccg ttgccgacca
      841 gcttgaagcc ggagaggctc ttgctcagga cctggttctc gttcgggcag tagaggaaga
      901 cggccggggc cttctcgctc agcaggtact ggagcttgtc gtaggtcacc ttccggtcgg
      961 cgtaggcagt ctggccgcgg ccggcgtcga ggaggccgtc gacggcggcg tccttgaaga
     1021 ggaagttgtt caccccgccg gtcgagtgga aggtcctgta gaggaagcgg tcgggctcgg
     1081 cgctgccgcc gcggagctcg atcatcgtgt cgaagtcgcg cttcacccac ttgtcgatgt
     1141 agacgcccca ctcgacgacc tcgagctcgg cggtgatgcc gatcttcttg agctcgtcct
     1201 gggcgacctg ggccaccgcg agcccgccct cgtaggtcga ggagcaggtg atcttcatgg
     1261 tgaagccgtt cgggaagccg gccttcgcga ggaggctctt ggcctgggcg aggtcggggc
     1321 cggtgaaggg cagggtcgag ggggccgccg cccacttggt ggccgcgatc ggcagggggc
     1381 cggtgacctt gcccatgccg aactcggcga tggtgaggat ctcgggggcg tcgagggcga
     1441 gggagacggc ctggcggacc agcacgttgt cgaagggctt gcgggtcgtg ttgaagctga
     1501 agatccgcat gttgagcccg ggcttgctca tgaccgtaac cttggggtcc ttcgcggcct
     1561 ggcggatgat ggcgccgtcg ttgatcgtgg ccatgtcgag ctcgcccgac ttcaccccgg
     1621 cgaggaggga ggcctgctcg gggatcacgc ggaagatcac cttgtcggcg gtgggggccc
     1681 ccttctcgaa gtagtcaggg ttcctcacga gggtcatcga gttgtcggcg acccattcct
     1741 tgagcatgaa gggtccggtc ccggcctcga ccttctggag gttgccgttg gccgcgacgg
     1801 cctccttggc gacgatcgcg aggttgttcg aggacaggcc gtcgaggagg gagggcaggg
     1861 gcccggagag cgtgagcttc accgtgtact tgtccacggc ctcgatcgcc gtgacggtcg
     1921 cgatgtagga gcggccgggc gaggcggtct tcgggtcgag cacgcggttg agcgagaaca
     1981 ccacgtcgtc cgaggtcatc tcgcggccgt tgtggaactt gacgcccttg cggaggtgga
     2041 agacgtaggt ggtgttgttg gggttgtccc aggactcggc caggtcgggg acgatcgcca
     2101 tgctgtcgtc gatgcggacg agccggttgt agagcaggtc gaggcggcgg tgcgaggaga
     2161 aggccgtcac gatgtgggga tccaggccga cggcctcctg gtcgacgccg atcgtgatca
     2221 ccttctccgc gggcgcggcg aaggcgccgg ccgtcgggat cagggccgcc accgcgaggg
     2281 cgacgagcag gatcatgagt ctcttcatca ttgcctcctt atggactttc acatgagctc
     2341 gatcacagtg taatggagcc gaggacgact ggggagcggg cccccgtgac cgccggcagg
     2401 ttgcccggcc ttccgaccag ggactcgtgg ccgagcaccg cgaaggccat ggcctccctg
     2461 gcctggtcgg gcaccccgtg ggcggccgtc gtcgtgaccc tgatgccagc cggcagcctc
     2521 tcctggatca tccccaggag gcaggggttg agggccccgc cgccggcgac gaggacggcc
     2581 gcgatgggcg tccggggcag gatgaagtcg cggtaggcag cggctatcga ctcggcggtg
     2641 aaggcggtcg ccgtggccac cgtgtcggcg taggagaggc cccgccccct g
//
LOCUS       DASSQV010000230         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1300507, whole genome shotgun sequence.
ACCESSION   DASSQV010000230 DASSQV010000000
VERSION     DASSQV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300507"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VFL04_03970"
     CDS             complement(<1..1192)
                     /locus_tag="VFL04_03970"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838890.1"
                     /translation="MGISATLRFRARSGAAALGLVLLLGLAPLGAQAPAPKLFIGLFK
                     ENVVAVLDTASNRIVDRIPIPAGPHGLAITPDGTTLFASSDGDSKVSVIDTATDKVTQ
                     TIEVGNSPHGLAMAPDGRLLLAAVFGASSVVFIDTATKQVVGRVAVPSPHNIAISPDG
                     RTAYVAAQAKGSLGLAILDLPSMAQRGFFPLDKTPRALSFSPDGRKLYYTLAGSDSVQ
                     DLATADNVLEAQVPVGASPHLPLFSADGKSALVVSQGPGELYIIDTARGQVSGKVKVG
                     AMPHWIALSADGRTAYVTNEGSGDLCLVDLASRSLVATIPLGQAPRKLVIQPSGGARV
                     SISGFSFPDRVSVAPGGTVTWTNRDAVTHTVTADDRSWDSGELAQGQSYSVVFRTPGS
                     YAYHC"
     gene            complement(1238..2236)
                     /locus_tag="VFL04_03975"
     CDS             complement(1238..2236)
                     /locus_tag="VFL04_03975"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7838891.1"
                     /translation="MKAMTGFKDDEELIHEVAEGGAEALTQLYRRYAALVFHLAFQSL
                     GRESAEDLVQDSFLAVWSKAGTFDPKRGSFRAWLLQITHFRIINELRRRSRRPKLDPD
                     SEDLDSFPDGGDEPALAAWRGFQKEAIRAAVERLPPAQGRALSLAFFEDLSHDQVAEA
                     LRLPLGTVKTRIRSAVRRLRSILAAVAAVAILAGLAGLGLGFRGQLVAASRAARALAF
                     VTASDITTIHLSAGPGLPSATHGSYRGRAGTDMAVMALHNLPEAPKGMAYQAWVRSDG
                     AWMSLGLARPDAAGNAMIVAETSGIGRLPLEVQVTLEPGRGSPSPSGAVTVFWKGE"
BASE COUNT          356 a          793 c          857 g          384 t
ORIGIN      
        1 agcagtgata ggcgtagctc cctggcgtcc ggaacaccac actgtagctc tggccctggg
       61 cgagctctcc cgaatcccag ctcctgtcgt cggcggtcac cgtgtgagtc acggcgtcgc
      121 ggttggtcca ggtgacggtg ccgccggggg cgacgctgac cctgtcggga aagctgaagc
      181 ccgagatcga gaccctcgcc ccaccgctgg gctggatcac gagtttcctc ggggcctgtc
      241 caagggggat ggtggcgaca aggctgcggc tggccaggtc gaccaggcag agatcgcccg
      301 agccctcgtt cgtcacgtag gccgtcctgc cgtcggcgct cagggcgatc cagtgcggca
      361 tggcgccgac cttgaccttg cccgacacct ggcccctcgc cgtgtcgatg atgtagagct
      421 cgcccgggcc ctggctcacg acgagggcgg atttcccgtc ggccgagaag agggggaggt
      481 ggggcgaggc gccgacgggc acctgggcct cgaggacatt gtcggcggtg gcgaggtcct
      541 ggaccgagtc agagcccgca agggtgtagt agagcttcct tccgtccggg ctgaagctca
      601 gggcccgcgg ggtcttgtcc agggggaaga agccccgctg cgccatgctg ggcaggtcga
      661 ggatggccag gccgagcgag cccttggcct gggccgcgac ataggccgtc ctcccgtcgg
      721 ggctgatcgc aatgttgtgc ggactcggca cggccaccct tccgacgacc tgcttcgtgg
      781 cagtgtcgat gaagaccacc gaggatgccc cgaacacagc ggccagaagg agcctgccgt
      841 cgggcgccat ggccaggcca tggggcgagt tgcccacctc gatcgtctgg gtcaccttat
      901 cagtggccgt gtcgatcacg ctcactttgc tgtccccgtc gctgctcgcg aaaagggtcg
      961 tgccgtcggg ggtgatggcc aggccgtggg ggccggccgg gatcgggatc ctgtcgacga
     1021 tgcggttcga tgccgtgtcc aggaccgcta ccacgttctc cttgaagagg ccgatgaaga
     1081 gcttgggcgc gggggcctgg gccccgagcg gggccaggcc gagcagcagg acaagaccca
     1141 aagctgccgc gccagagcgg gcgcggaatc gaagcgtggc ggaaataccc atgaacaacc
     1201 tcccatcagg ggccccgcct tggcggggcc ccgtttgtca ttctcccttc cagaagaccg
     1261 tgaccgcccc cgagggggag ggactgcccc tgccgggctc gagcgtcact tggacctcga
     1321 gcggcagccg tccgatgccg cttgtctcgg cgacgatcat ggcgttgccc gcggcgtcgg
     1381 gtctggccag accgagggac atccacgccc catcgctcct gacccaggcc tggtacgcca
     1441 ttccctttgg cgcctcgggc aggttgtgga gggccatgac ggccatgtcg gtgcccgccc
     1501 tgccccggta ggagccgtgg gtagcgctcg gcaggccggg ccccgccgag aggtggatgg
     1561 tcgtgatgtc gctcgccgtc acgaaggcga gggcgcgcgc ggcgcgcgag gcggccacga
     1621 gctggccacg gaaacccagg cccaggccgg cgaggccggc caggatggcc accgcggcca
     1681 ctgcggcgag gatggagcgg agcctgcgga cggccgacct gatccttgtt ttcaccgtgc
     1741 ccaggggaag cctcagggcc tcggctacct ggtcatggct caggtcctcg aagaaggcga
     1801 ggctgagggc ccggccctgg gccgggggca ggcgctcgac agcggccctg atggcctcct
     1861 tctggaagcc gcgccaggcc gcgagggcgg gctcgtcgcc cccgtcgggg aagctgtcga
     1921 ggtcctccga gtcggggtcc agcttgggcc tcctgctccg gcgcctgagc tcgttgatga
     1981 tcctgaagtg tgtgatctgg aggagccagg ccctgaagga gccccgcttc gggtcgaagg
     2041 tcccagcctt gctccagacc gcgaggaagc tgtcctggac caggtcctcg gccgactcgc
     2101 ggcccaggct ctggaaggcg aggtggaaga cgagggcagc gtagcgtctg tacagctggg
     2161 tcagggcctc ggcccctccc tcggccacct cgtggatgag ctcctcgtcg tccttgaacc
     2221 cggtcattgc cttcatctcg tccccttcga tccaggagat acaccgcgga acctgccctg
     2281 gattgcggga cccgctgccg gaacgccttt tcccgaatat atgcagggcc gggacgaatg
     2341 ccgaaacccg gcctagctcg ctcgggcgga ctcgcgtata ctctggcctt
//
LOCUS       DASSQV010000231         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_803682, whole genome shotgun sequence.
ACCESSION   DASSQV010000231 DASSQV010000000
VERSION     DASSQV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803682"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..835
                     /locus_tag="VFL04_03980"
     CDS             308..835
                     /locus_tag="VFL04_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET7838892.1"
                     /translation="MAEQLDQIDRKLIGLLQADARLSNAELADKVGLTTSSVHERVKK
                     LEKRGVITGYVATVDAEKLGKPMLAFVRATVAAEDHEGTVSFHDRIVAACRAESDILE
                     CHNVAGEDCFIIKVRAESPKDLERILQTIRSTVRSSRSVTNIVLSTYKESTRVEPAPA
                     EAQALENPVPREVQA"
     gene            832..1113
                     /locus_tag="VFL04_03985"
     CDS             832..1113
                     /locus_tag="VFL04_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838893.1"
                     /translation="MSTATISLSFNDFARTASEGEALQALRIALLYGEQLRIPSSSEW
                     LRILAGKAWVSYSGEDHLLGEGEELDLPRAKGGAVISALGGESLFFEVK"
     gene            1140..>2036
                     /locus_tag="VFL04_03990"
     CDS             1140..>2036
                     /locus_tag="VFL04_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7838894.1"
                     /translation="MGILNIGAVAALATALSWTITAIAFEYAGKRIGAFALNFLRLVL
                     GMVFLGLYCLVVRGSFFPLDAPASAWLWLSASGIVGLVVGDLLLFQAFIDIGSRVSML
                     LYASAPALTAVLGFGILGERLAGLALLGMALTLAGIAIVVLGRGEGQLEAECPEGEAK
                     CAPRARRIRGLACALGGALGQAAGLILSKVGAGSMDPFAGTQIRVSAAVLGFAVVLAA
                     SGAWRGTFRALKDGKAIASLGLGSFFGPFLGVSLSLLAVQTTKAGLAAAIMSIVPILI
                     IAPSALIYKERVQGRDVLGSVVA"
BASE COUNT          315 a          703 c          683 g          335 t
ORIGIN      
        1 cggcgagccg ggcgaggtcg cgagtttcca tgtgggacga ctatggcctc gcggggggcc
       61 aaggtaaagg ggacgggagt gcctcgaagc ctgttggtgg tcggccccgc gccgccctgt
      121 gcccccggcc agggtaggcg ggacctggcg ggcttcgcgg gccgctcggg ccgctcgggc
      181 cactcgggcc gcacggccat cccccgagtc gggttgtccg acgccaatcg acgcaatttt
      241 tcctgtgatt tcgccaatct gccaaataaa atttgaccac atcgggatat cgcaggatat
      301 tattcgcatg gcagaacagc tagaccagat agacaggaag ctcatcggcc tcctccaggc
      361 cgacgcaagg ctttccaacg ccgagctcgc cgacaaggtg gggcttacga cctcatcggt
      421 gcacgagagg gtgaagaagc tcgagaagcg gggcgtcatc accggctatg tggcgaccgt
      481 ggacgcggag aagctcggca agcccatgct cgccttcgtg cgcgccacgg tggccgccga
      541 ggaccacgag ggaacggttt ccttccacga caggatcgtc gcggcctgcc gggccgagag
      601 cgacatcctc gagtgccaca acgtcgccgg ggaggactgt ttcatcatca aggtccgcgc
      661 cgagagcccc aaggacctcg agcggatcct ccagacgatc cgctccacgg tgcgcagcag
      721 ccgttcggtg accaacatcg tcctgtccac gtacaaggaa tcgacaaggg tggagccggc
      781 gcccgcagag gcccaggctc tcgagaaccc agtcccaagg gaggtgcagg catgagcacg
      841 gcgacgatca gcctgagttt caacgatttc gcgcggaccg cctcggaggg cgaggccctc
      901 caggcgctcc gcatagccct tctctacggc gagcagctcc gcatccccag ctcctcggag
      961 tggctccgca tcctggccgg caaggcctgg gtgagctatt cgggcgagga ccacctcctc
     1021 ggcgaggggg aggagctcga cctgcccagg gcgaagggcg gggcagtcat ctcggccctc
     1081 ggcggggagt ccctcttctt cgaagtgaag tagaaacacc tatcagaaat ggagacgaca
     1141 tgggcatcct gaacatcggc gccgtcgccg ccctggcgac ggccctctcc tggacgatca
     1201 cggccatcgc cttcgagtac gcgggcaagc gcatcggggc cttcgccctc aacttcctca
     1261 ggcttgtcct gggcatggtc ttcctgggcc tctactgcct cgtcgtccgg ggctccttct
     1321 tccccctgga cgccccggcc tcggcctggc tctggctctc ggcctcgggc atcgtgggcc
     1381 tcgtggtagg cgacctcctg ctcttccagg ccttcatcga catcgggtcc agggtatcga
     1441 tgctcctgta cgccagcgcc ccggccctga ccgccgtcct cggcttcggg atcctcgggg
     1501 agcgcctggc cggcctggcc ctcctcggca tggccctgac cctcgcgggc atagccatcg
     1561 tcgtgctggg ccgcggcgag gggcagctcg aggccgagtg ccccgagggc gaggccaagt
     1621 gcgcgccgag ggcgaggcgg atcaggggcc tcgcctgcgc cctgggcggg gccctcggtc
     1681 aggctgcggg cctcatcctg agcaaggtgg gggcggggag catggacccc ttcgccggca
     1741 cccagatcag ggtgagcgcc gcggtcctcg gcttcgcggt ggtccttgcc gcctcggggg
     1801 cctggagggg caccttcagg gccctcaagg acgggaaggc catagccagc ctcggcctcg
     1861 gctccttctt cggccccttc ctcggggtct cgctgagcct cctcgccgtg cagacgacca
     1921 aggccggcct cgcggcggcc atcatgtcga tcgtccccat cctcatcatc gccccctcgg
     1981 ccctcatcta caaggagagg gtccagggac gggatgtcct cggctcggtc gtggcc
//
LOCUS       DASSQV010000232         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1548682, whole genome shotgun sequence.
ACCESSION   DASSQV010000232 DASSQV010000000
VERSION     DASSQV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548682"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1390)
                     /locus_tag="VFL04_03995"
     CDS             complement(128..1390)
                     /locus_tag="VFL04_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7838895.1"
                     /translation="MKTKAVRIHGVDDLRLEEFELPAIKDDEILARVVSDSICMSSHK
                     LAMQGDKHKRVRSPLAARPVIIGHEFCGELVEVGAKWSRQFKPGQRFAIQPALNYKGT
                     LWAPGYSYEHIGGDATYIVIPAEVREMGCLLEYKAEAFFMGSLAEPVSCIVGAYHAQY
                     HTRGGSYVHEMGIVEGGSLALLASVGPMGMGAIDYAMHADRRPSRVVVTDIDEARLAR
                     AASIFTVAEAKRCGVELHYVNTKDMADPVAHLKGLNGGRLFDDVFVFAPVKQVVELGD
                     RLLGPDGCLNFFAGPTDTGFSASFNFYNVHYESHHLVGTSGGNTDDIVESLEMMADGR
                     LNPVAMVTHVGGLNAVPETTISLDKIPGGKKLIYTHKRLDLVALADFAERGRKEPFYA
                     ELARIVAANNMLWCKEAEDYLLAHAPEI"
     gene            complement(1404..>2160)
                     /locus_tag="VFL04_04000"
     CDS             complement(1404..>2160)
                     /locus_tag="VFL04_04000"
                     /inference="COORDINATES: protein motif:HMM:NF044822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HET7838896.1"
                     /translation="FVLNSTGTWCVLMHPQEKYGFSRSELGKVVFYNRSAFNKPVKTA
                     IFLGGMEYETWSALVAKAAGGNCVEPGPEDYLAVLEGGSEFILPELVPGSGQFPGSRP
                     RAVEDGREYALGDLQSGGPLPAFLSDSRRAMAVLNLSLAIQTLVALGRAGLEPGAQVF
                     TEGGFRRNADYNAILAAALPANPVYLTDIVEATAAGAAMTALVALDREAGGRRGIEGI
                     ARDFTVEYLSVAPMPGMDGLGAYTQQWLERVAR"
BASE COUNT          342 a          689 c          755 g          374 t
ORIGIN      
        1 cgggttcgat ccacgaatat cagagtgatc aaccgtcgtc gaaagcttct tcggcccttg
       61 ctcgaattgc cctgcctgca aagaaaatga gaaaggccga agatgcctgt tgacgggtca
      121 cttcatatca gatctcgggc gcgtgggcga ggaggtagtc ctcggcctcc ttgcaccaga
      181 gcatgttgtt ggccgcgacg atccgcgcga gctcggcgta gaagggctcc ttcctgccgc
      241 gctcggcgaa gtcggcgagg gccacgaggt cgaggcgctt gtgggtgtag atgagcttct
      301 tcccgcccgg gatcttgtcg aggctgatcg tcgtctcggg cacggcgttg agcccgccca
      361 cgtgggtgac catggcgacg gggttcaggc ggccgtcggc catcatctcc agggactcca
      421 cgatgtcgtc ggtgttgccg cccgaggtgc ccaccaggtg gtgggattcg taatgcacgt
      481 tgtagaagtt gaagctggcc gaaaagcccg tgtcggtggg gcccgcgaag aagttgaggc
      541 agccgtcggg gccgaggagc ctgtcgccga gctcgaccac ctgcttcacc ggggcgaaga
      601 cgaagacgtc gtcgaagagc ctgccgccgt tcaggccctt gaggtgggcc acgggatcgg
      661 ccatgtcctt cgtgttcacg tagtggagct cgaccccgca gcgcttggcc tcggccacgg
      721 tgaagatcga ggcggcgcgg gctagcctgg cctcgtcgat gtcggtcacc accacccggg
      781 agggccttcg gtcggcgtgc atggcgtagt cgatggcccc catgcccatg ggcccgacgc
      841 tcgcgaggag ggccaggctc cccccctcga ctatgcccat ctcgtggacg taggagccgc
      901 ccctggtgtg gtattgggcg tggtaggcgc caacgatgca cgacaccggt tcggccaggg
      961 agcccatgaa gaaggcctcg gccttgtact cgaggaggca gcccatctcc ctgacctcgg
     1021 ccgggatgac gatgtaggtg gcgtcccctc cgatgtgctc gtaggagtag ccgggcgccc
     1081 agagcgtacc cttgtagttg agggccggct ggatggcgaa cctctgtccc ggcttgaact
     1141 gccggctcca tttggcgccg acctcgacca gctcgccgca gaactcgtgg ccgatgatca
     1201 cgggcctggc ggcgagaggc gagcgcacgc gtttgtgctt gtcgccctgc atggcgagct
     1261 tgtggctcga catgcagatc gagtcggaga ccacacgcgc gaggatctcg tcgtccttga
     1321 tggcggggag ctcgaactcc tcgagcctga ggtcgtcgac cccgtggatc cgaaccgctt
     1381 tggtcttcat catgccctcc gattcatcgc gcgactcgct cgagccactg ctgggtatag
     1441 gcgccgaggc catccatgcc gggcatgggc gccacgctga ggtactcgac cgtaaagtcc
     1501 cgggctatgc cctcgatccc gcgcctgcct cccgcctcgc ggtccagggc gaccagggcc
     1561 gtcatggcgg cccccgcggc cgtcgcctct acgatgtctg tcaaatatac gggattggcc
     1621 ggcagggcgg cggcgagtat ggcgttgtag tcggcgttcc gccggaagcc cccctcggtg
     1681 aagacctggg cccccggctc gaggccagcg cggccgaggg cgacgagggt ctggatcgcg
     1741 agggagaggt tgaggacggc catggcgcgg cggctgtcgg acaggaaggc cggaaggggg
     1801 cccccgctct gaagatcgcc caaagcgtac tcgcggccgt cctcgacggc gcggggcctc
     1861 gagccgggga actggcccga gccggggacg agctcgggca ggatgaactc cgagcccccc
     1921 tcgagcacgg cgaggtagtc ttcggggccc ggctccacgc agttcccccc cgcggccttc
     1981 gccaccaggg ccgaccaggt ctcgtactcc atgccgccga ggaagatcgc cgtcttcacc
     2041 ggcttgttga aggccgagcg gttgtagaag accaccttgc cgagctcgct gcgggagaag
     2101 ccgtacttct cctgggggtg catgaggacg caccaggtgc ccgtcgagtt gaggacgaag
//
LOCUS       DASSQV010000233         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1921862, whole genome shotgun sequence.
ACCESSION   DASSQV010000233 DASSQV010000000
VERSION     DASSQV010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921862"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..120
                     /locus_tag="VFL04_04005"
     CDS             7..120
                     /locus_tag="VFL04_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HET7838897.1"
                     /translation="MVAITAGELHFGPWERIFYGEFDGRRRKRVLIKIIGE"
     gene            178..250
                     /locus_tag="VFL04_04010"
     tRNA            178..250
                     /locus_tag="VFL04_04010"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Phe,seq:gaa)
     gene            395..1366
                     /gene="galE"
                     /locus_tag="VFL04_04015"
     CDS             395..1366
                     /gene="galE"
                     /locus_tag="VFL04_04015"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET7838898.1"
                     /translation="MKILVVGGAGYIGSHVAREFLDSGHQVDVYDNLSTGSRENLFPE
                     GRFFEGDILDYPRLREAMGHGYGAVIHLAAAKAAGESMLKPEKYSLQNLNGTVNILNA
                     ALDAGILRLVFSSSAATYGEPKYLPIDEKHPTEPENYYGFTKLEIERILAWYDRLKGM
                     RFAALRYFNAAGYDVRGRIAGLEKNPANLIPVIMETAVGMRRELKVFGNDYPTKDGTG
                     VRDYVHVSDLARGHLAALEWISRNDRSLTVNLGSGEGLSVLEILECARRISGRPIPAE
                     MVARRPGDPARLVASSGLAKEVLGWEARESDLETLVASTWKAYSRHE"
     gene            1425..2654
                     /locus_tag="VFL04_04020"
     CDS             1425..2654
                     /locus_tag="VFL04_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HET7838899.1"
                     /translation="MADKSAIFSYLDSSTPGIVELESLLTSIPAIAPDSGGEGEMKKA
                     EALIAWLAKKGITAIERYDAPDPRVPGGKRPNVIATIPGNRADDRLWIMSHLDVVPEG
                     ARAMWSSDPYNVVHKDGKVIGRGVEDNQQGLVSSVFAALAFIEKGQKPERTLKLLFVA
                     DEEVGSKFGIQYLLTQKGIFRKDDLIVIPDGGSADGSEIEVAEKNICWLKIVTKGKQT
                     HAAMPDAGHNAFLAACDLALRLHAMEQDTFTARDGLFEPDRSTINPTKKEANVPNVNT
                     IPGDDVFCVDMRMLPQYPLDAVLAEVEKRMRIVEKIHEVSMHFEVIQRNESKATAPDA
                     PVVRHLSKAVKSVYGVSAKPIGIGGGTVGAYLRNAGFDCVVWARMNETAHQPNESAVV
                     ANIIGDAKVFASLLLGE"
BASE COUNT          525 a          963 c          900 g          440 t
ORIGIN      
        1 gaggtcgtgg tcgcgatcac ggctggcgag ctccatttcg gcccatggga acgcatcttt
       61 tacggcgagt ttgacggtcg ccgcaggaag cgcgtcctca tcaagatcat aggggagtag
      121 atctagggcg gggttgacga atgcctaccc ggccaggtat aaaactatcc gatcgaagcc
      181 gctgtagctc agttggtaga gcagtggact gaaaatccac gtgtcatcag ttcaattctg
      241 atcggcggca cgacaaggaa agccgacccc ccgggggtcg gctttttcgt attcctgcga
      301 tcggtcatgg acccttgtcg ggagccggcg ctatactgcg ccctaggccg cgcggccggg
      361 tcttgggccc gcccggcgcc cggaaggagc cgtcttgaag atcctcgtag tgggtggagc
      421 gggctacata ggcagccacg ttgcgcgcga gttcctcgac tccggacatc aggtcgacgt
      481 gtacgacaac ctctcgacgg gcagccgcga gaaccttttc cccgaagggc gcttcttcga
      541 gggggacatc ctcgactatc cgcggctccg cgaggccatg ggccacggct atggggccgt
      601 catccacctc gccgccgcca aggctgccgg ggagtcgatg ctgaagccgg agaagtactc
      661 gcttcagaac ctgaacggca ccgtgaacat cctgaacgcc gccctcgacg ccggaatcct
      721 caggctggtc ttctcatcct cggccgccac ctatggtgag ccgaaatacc tccccatcga
      781 cgagaagcac cccaccgagc ccgagaacta ctacggcttc accaagctcg agatcgagcg
      841 catcctcgcc tggtacgacc ggctcaaggg gatgcgcttc gccgccctcc gctatttcaa
      901 cgccgcgggc tacgacgtgc gcggaaggat cgccgggctc gagaagaacc ccgccaacct
      961 catcccggtg atcatggaga ccgccgtcgg catgaggcgg gagctcaagg tcttcggcaa
     1021 cgactacccc acgaaggacg gcacgggggt gcgcgactat gtccacgtca gcgacctcgc
     1081 ccgcggccac ctcgccgccc tcgagtggat cagccgcaac gacaggagcc tcacggtcaa
     1141 cctcggttcg ggggagggcc tctccgtcct cgagatcctc gaatgcgcca ggaggatctc
     1201 cggccggccg atccccgccg agatggtcgc ccgccgtccc ggcgaccccg caaggctcgt
     1261 cgcctcgtcg ggcctcgcga aggaagtcct gggctgggag gccagggaat ccgacctcga
     1321 gaccctggtc gccagcacct ggaaggccta ttccagacac gaatgacatc gtttccggcg
     1381 ggccggcatc gctgcccgcg tcaaggcaca tgaaaggagc ccctatggcc gacaagtccg
     1441 cgattttttc ctatctcgat tcctcgacgc ccggcatcgt cgagctggag tccctcctca
     1501 cctcgattcc ggcgatcgcc cccgactcgg gcggcgaggg ggagatgaag aaggccgagg
     1561 ccctcatcgc ctggctcgcc aagaagggca tcaccgcgat cgagcgctat gacgcccccg
     1621 acccgagggt gccgggcgga aagcggccga acgtgatagc gaccatcccc ggcaaccggg
     1681 ccgacgacag gctctggatc atgagccacc tcgacgtggt gcccgagggg gcccgtgcga
     1741 tgtggagctc cgacccctat aatgtcgtac acaaggacgg caaggtcatc ggccgggggg
     1801 tcgaggacaa ccagcagggc ctcgtgagct cggtcttcgc cgccctcgcc ttcatcgaga
     1861 aggggcagaa gcccgagcgc accctgaagc tgctgttcgt cgccgacgag gaggtcggct
     1921 ccaagttcgg gatccagtac ctcctgaccc agaaggggat cttccgcaag gacgacctca
     1981 tcgtgatccc cgacggcggc tcggccgacg gctcggagat cgaggtggcc gagaagaaca
     2041 tctgctggct caagatagtc acgaagggca agcagaccca cgccgccatg cccgacgccg
     2101 gccacaacgc cttcctcgcg gcctgcgacc tcgccctccg cctccacgcg atggagcagg
     2161 acaccttcac cgcccgggac gggctgttcg agcccgacag gagcacgatc aaccccacaa
     2221 agaaggaggc caacgtcccg aacgtcaaca cgatccccgg cgacgatgtc ttctgcgtcg
     2281 acatgaggat gctcccccag tatcccctcg atgccgtcct cgccgaggtc gagaagcgca
     2341 tgcggatcgt cgagaagatc cacgaggtgt cgatgcattt cgaggtgatc cagcgcaacg
     2401 agtccaaggc cacggccccc gacgcccccg tcgtgcggca tctcagcaag gccgtgaagt
     2461 cggtgtacgg ggtctcggcg aagccgatcg gcatcggcgg cggcacggtg ggagcctatc
     2521 tccggaacgc cggcttcgac tgcgtcgtct gggcgaggat gaacgagacc gcccaccagc
     2581 ccaacgagag cgcggtggtg gccaacatca tcggcgacgc gaaggtcttc gcgagccttc
     2641 tgctcgggga gtaggggcgc tttgcccgcg gtggccgatc gccgccgcgg gccccgctac
     2701 cccggccagc gactgctgca tcgccaccgc tgtaccgcta ctgcgggcgc tgtcctgctg
     2761 aagtctggcc ggggcccgag gcctcgtcga gcacgatgag gacccggccc tccctcagga
     2821 ggagctgg
//
LOCUS       DASSQV010000234         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_928311, whole genome shotgun sequence.
ACCESSION   DASSQV010000234 DASSQV010000000
VERSION     DASSQV010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928311"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFL04_04025"
     CDS             complement(<1..631)
                     /locus_tag="VFL04_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7838900.1"
                     /translation="MKTIRLTMAQALVRFLDNQYLEVDGEERKFVAGVFGIFGHGIVV
                     GLGEALAASGHGLRFYQGKNEQGAAHAAMAFAKQSDRHRIMAVTASIGPGSLNMVTAA
                     GTATVNRIPVLFLPADSFACRQPDPVLQQVEQPGDLNVTANDAFKAVSRYWDRVSRPE
                     QLMTAMLNAMRVLTDPAETGAVTVALPQDVQGEAYDYPEEFLAKRVHHLE"
     gene            complement(634..1473)
                     /locus_tag="VFL04_04030"
     CDS             complement(634..1473)
                     /locus_tag="VFL04_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HET7838901.1"
                     /translation="MILKHKGPFARGYEAIVERGGENADMLMDFAVLSLGPGESWASG
                     AADEKAILLVSGELELEWSGEPGGRARAARGSFLDEAPTVLHLSAGSEARLVAGGRGA
                     ELAVMRTDNPRGFPPRLWLPKDCRDEERGKGTMRETSTRIVRTVFDDSNAPAANLVVG
                     EVVAAPGKWSSYPPHHHPQPEIYHYRFQPAQGFGLTVIGEEAHLLGQNDTVLIREGQV
                     HPQVAAPGYAMWYIWVIRHLDGNRYVKPKFVAEHEWVQDPDAAIWLAPSERSGPGPGA
                     KGR"
     gene            complement(1484..>2063)
                     /locus_tag="VFL04_04035"
     CDS             complement(1484..>2063)
                     /locus_tag="VFL04_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7838902.1"
                     /translation="EPHLVRISSRDPAPPPMAYVRGSGGIFLDMTIHDFDMARFQAGS
                     EVVEVYAAGAVLVDKAIGDAGDVDTAVITLKFANGALGVIDNSRKAAYGYDQRVEVFG
                     SKGAAAAENDAPSTVKLSNEAGVSGEKPMYFFLERYKEAFIAEMRAFLDAVANDKACP
                     VTGEDGLQDLLVGLAAKKSVAEKRPVKVSEIG"
BASE COUNT          271 a          698 c          749 g          345 t
ORIGIN      
        1 gctcgaggtg gtggacccgc ttcgcgagaa attcctcggg gtagtcgtag gcctcgccct
       61 ggacgtcctg gggcagggcc acggtcacgg cccccgtctc ggcggggtcg gtgaggacgc
      121 gcatcgcgtt cagcatcgcg gtcatgagct gctcgggtct cgacacgcgg tcccagtagc
      181 ggctcacagc cttgaaggca tcgttggcgg tgacgttgag gtcgcccggc tgctcgacct
      241 gctgcagcac cgggtcgggc tggcggcagg cgaagctgtc ggcggggagg aagaggacgg
      301 ggatccggtt caccgtggcc gtccccgcgg cggtgaccat gttgagcgag cccgggccga
      361 tcgaggcggt gacggccatg atcctgtgcc ggtcgctctg tttggcgaag gccatcgctg
      421 cgtgggccgc gccctgctcg ttcttgccct gatagaagcg caggccgtgg cccgaggcgg
      481 ccagggcctc gccgaggccg acgacgatgc cgtggccgaa gatcccgaag acgccggcga
      541 cgaacttgcg ctcctcgccg tcgacctcga ggtactggtt gtcgaggaag cggacgaggg
      601 cctgggccat ggtgaggcgg atcgtcttca tgctcatctt cctttcgccc cgggcccggg
      661 cccggagcgc tccgagggcg cgagccagat cgccgcgtcg gggtcctgga cccactcgtg
      721 ctcggcgacg aacttcggct ttacgtagcg gttcccgtcg aggtggcgga tcacccagat
      781 gtaccacatc gcgtagccag gggccgcgac ctgggggtgg acctggccct cgcggatgag
      841 gaccgtgtcg ttctggccga ggaggtgggc ctcctcgccg atcaccgtga ggccgaagcc
      901 ctgggcgggc tggaagcggt agtggtagat ctcgggctgg ggatggtggt ggggagggta
      961 gctcgaccac ttgcccgggg ccgcgaccac ctcgccgacg acgaggttgg cggccggggc
     1021 gttcgagtcg tcgaagacgg tccgcacgat cctcgtgctg gtttcgcgca tcgtgccctt
     1081 gccccgctcc tcgtccctgc agtccttggg cagccagagc ctcgggggga agccgcgcgg
     1141 gttgtcggtc cgcatgactg ccagctcggc cccccgcccc ccggccacga gcctcgcctc
     1201 cgagcccgcg ctcaggtgga ggacggtcgg ggcctcgtcg aggaagctcc cgcgcgccgc
     1261 cctggccctg ccgcccggct ccccgctcca ctcgagctcg agctcgcccg agacgaggag
     1321 gatggccttc tcgtccgcag ccccggaggc ccagctctcg ccggggccga ggctgaggac
     1381 ggcgaagtcc atgagcatgt cggcgttctc gcctcccctc tcgacgatgg cctcgtagcc
     1441 tctcgcgaag gggcccttgt gcttgaggat catggccgcc ctgtcatccg atctcggaga
     1501 ccttgacggg ccgcttctcc gcgaccgact tcttggcggc caggcccacc aggaggtcct
     1561 ggagcccgtc ctccccggtc acggggcagg ccttgtcgtt ggctaccgcg tcgaggaagg
     1621 cgcgcatctc ggcgatgaag gcctccttgt agcgctcgag gaagaagtac atcggcttct
     1681 cgccggagac cccggcctcg ttggacagct tcacggtgct cggcgcgtcg ttctcggcgg
     1741 cggccgcgcc cttggagccg aagacctcga cgcgctggtc gtagccgtag gctgccttgc
     1801 gcgagttgtc gatgaccccg agggccccgt tcgcgaactt gagggtgatg acggccgtgt
     1861 ccacatcacc ggcgtcgcct atcgccttgt cgacgaggac ggcccccgcg gcgtagacct
     1921 cgaccacctc gctccccgcc tggaagcggg ccatgtcgaa gtcgtggatg gtcatgtcga
     1981 ggaagatccc gccggagcca cgcacgtagg ccatcggcgg cggcgcgggg tcgcggctgg
     2041 agatgcgcac gaggtgcggc tcg
//
LOCUS       DASSQV010000235         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2047902, whole genome shotgun sequence.
ACCESSION   DASSQV010000235 DASSQV010000000
VERSION     DASSQV010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047902"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VFL04_04040"
     CDS             complement(<1..1033)
                     /locus_tag="VFL04_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838903.1"
                     /translation="MSEADGAGWKVGRSIILELDLAISIACARAGPGLGTGEIAALVA
                     GTPARLRREFVRMFGEPRRLHATMEAAALAAGVLEEDDIEAAAGAMRGLDLAGAVAAL
                     EDLRERRGIAGQAADAPAGTVTRSAVTLSPVARPPAGTVTRFVEAYVHLKAALYAELG
                     FAPASLGPLLAEARVDAERGALILAGGREGEAFWPWLDRFHSEVYLPWRQGREEAMLE
                     LERRASSELGAPERGTGIPPTAWLSEKNPLLRFGELGSSLREGSCRPLFWAEPFGIAD
                     LWVATPTGCIVSIAGPGPAFEAFLGRFEEAARKVQAIADPTRLVMLCLIRQIGMTNSD
                     MAAYLGIARP"
     gene            complement(1033..2352)
                     /locus_tag="VFL04_04045"
     CDS             complement(1033..2352)
                     /locus_tag="VFL04_04045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7838904.1"
                     /translation="MGVPTSRFLPSAVLRIGTAEAVSGVGDWVSMMAVLMILVFHGRG
                     GVKESSFVLLAGLVPMIFASPFAGRLADRFDGRRLMIASQLISGVTVTALAFAGSPAM
                     IYALLVIESLSMSLMVPARQSALPKLLPPAWLTRANAFILQLGSIVKIGAPVLAGALL
                     VFMPPRLAFFLDALSFAAAALILLGLPSLPPGLRGPGVSPQLPGERPSGDSHASGCAS
                     GASPRWRRPRVAPLPGLRVVFAAGFFATLVLIGFDVMASVYCRDVLRRGGAFFGLVCS
                     LVGAGSLAASLILGLGTVTRSLTRSRLRDVALGTFAAAFIPIAFILAGRLEPGLPVMG
                     LVTAGCLGGGFGLGLLNVQSAGLIQQAAPPERLGSVSGLFQSLCSAGQLGGALLTPIL
                     VPGVLSFAAYLGSGALGLAILAMALALQSAKPWGRPDAQALGLGKAE"
BASE COUNT          491 a          805 c          909 g          318 t
ORIGIN      
        1 tcggcctcgc gatgccgagg taggccgcca tgtcgctgtt ggtcatgccg atctgacgga
       61 tcaggcagag catgacgagg cgggtcggat cggctatggc ctggaccttc ctggcggcct
      121 cctcgaagcg gccgaggaag gcctcgaagg ccggtcccgg cccggcgatc gacacgatgc
      181 agccggtcgg agtggcgacc cagaggtcgg ctatgccgaa gggctcggcc cagaagagag
      241 gtcggcagct gccttcccgc aggctcgacc cgagctcacc gaagcggagg aggggattct
      301 tttccgacag ccaggccgtg ggcgggatcc ccgtgccgcg ctcgggggcg ccgagctctg
      361 aggaggctct cctctcgagc tcgagcatcg cctcctccct gccctgtctc caggggaggt
      421 aaacctcaga atggaagcgg tcgagccaag gccagaaggc ctcgccctcc cgaccgccgg
      481 cgaggatgag ggcgccccgc tccgcgtcga ccctggcctc ggcgagcaat gggccgaggg
      541 aagctggcgc gaaacccagc tccgcgtaga gggcggcctt caggtggacg taggcctcga
      601 caaaacgggt gactgtcccc gctggcgggc gggcgaccgg cgagagggtg accgccgagc
      661 gcgtgactgt ccccgctggg gcatcggcgg cttggcccgc aatgccccgc cgctcgcgga
      721 gatcctcgag ggcggccacg gcaccggcga ggtccaagcc gcgcatcgcg ccggcggccg
      781 cctcgatatc atcctcctcg aggaccccag ccgcgagggc ggccgcctcc atggttgcat
      841 ggaggcggcg aggctccccg aacatcctaa cgaactcgcg gcggagcctc gccggagtcc
      901 cggcgacgag cgccgcaatc tcccctgtgc cgaggccagg gcctgccctt gcacaggcga
      961 tggagatggc caggtcgagc tctaggatga tggatctgcc gaccttccag cctgcaccgt
     1021 ctgcttccga catcattccg ccttcccaag gcccagcgct tgggcatcgg gcctgcccca
     1081 gggcttcgcg gactgcagcg cgagggccat ggcgaggatg gcgagcccga gggcgccact
     1141 gccgaggtag gccgcgaagg agagaacgcc ggggaccagg atgggagtca ggagggcgcc
     1201 tccgagctga ccggccgaac acagggactg gaaaaggcca gagaccgaac cgagcctttc
     1261 tggtggggcc gcctgctgga tgagacccgc agattggacg ttgaggaggc ccagcccgaa
     1321 gcccccgcca aggcaaccgg cggtgaccag gcccatgacc gggagcccag gctccagcct
     1381 tccggcgagg atgaatgcga tcggtataaa agcagcggcg aaggtcccga gggcgacgtc
     1441 tcgcagacgc gaacgggtga gggaacgggt gactgtcccc agcccaagga tgagggatgc
     1501 ggccaatgag ccggcgccga ccagggagca gacgaggcca aagaaggccc cgccccgcct
     1561 gagcacgtcc ctgcagtaga ccgaggccat cacgtcgaag ccgatgagga cgagggtcgc
     1621 gaagaacccg gccgcgaaga ccacacgtag gccgggaagc ggcgccaccc gggggcgtct
     1681 ccagcggggg ctggctccgg aggcgcaacc ggaggcgtga ctgtcccccg agggtcgttc
     1741 ccccgggagc tgcggcgaga cgccgggacc ccggagaccg ggtggaaggg agggaaggcc
     1801 gagcaggatg agggccgccg ccgcgaaact gagggcgtcg aggaagaagg ccagacgagg
     1861 aggcatgaat acaagaaggg cgcccgccag gaccggggct ccgatcttca ctatcgatcc
     1921 cagctgcagg atgaaggcat tcgcccgagt gagccaggcg ggaggcagga gttttgggag
     1981 agcggactgg cgtgccggga ccatgagtga catggacagg gattcgatga ccagcagggc
     2041 atagatcatg gcgggcgagc ccgcgaaggc gagagcggtg acagtcaccc ccgaaatgag
     2101 ttggctcgcg atcatcagtc ttcggccatc gaagcggtcg gcaagcctac cggcgaaggg
     2161 gctcgcgaag atcatcggca ccagtccggc aaggaggaca aaggagctct ccttgacccc
     2221 acccctgccg tggaagacga ggatcatgag caccgccatc atggagaccc agtccccgac
     2281 ccccgacacg gcctcggccg tgccgatccg gaggactgcg gacgggagaa aacgagaagt
     2341 tgggacgccc atctggattc cctcctctta aagcgtagcc caaagactac gctttaagaa
     2401 tgccacgaag ggtaacggga gtcaagatgc ctgagatctt ggtcatggac acgggcggcc
     2461 gcaattggga cgttaatcgg cccagggaca gtcaccgagg tgtttgtcac cgaggcgcca
     2521 ccg
//
LOCUS       DASSQV010000236         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2048244, whole genome shotgun sequence.
ACCESSION   DASSQV010000236 DASSQV010000000
VERSION     DASSQV010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048244"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFL04_04050"
     CDS             <1..201
                     /locus_tag="VFL04_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7838905.1"
                     /translation="VGAKYEIYTIMNRLVQQGMSIVMISSELPEILGMSDRVYVVASG
                     RITGELPAAEATQETIMRLATS"
     gene            243..1403
                     /locus_tag="VFL04_04055"
     CDS             243..1403
                     /locus_tag="VFL04_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7838906.1"
                     /translation="MRKNLREYGMYIALVIIMAIFSIQTKGMFISSRNIANLLNQTGY
                     IAVLAVGMTLVIVIRHIDLSVGFLAGFLGAVAAIGLASWHLNVWVVLVLVLALGVVAG
                     LVTAFPVARLGIPSFVASLAGWLIYRGALLLVTSSTGTIIIPNDTFNAIGNGYIPDIL
                     PADSAFLPGLHKLTLVLGLLAMVWFVIREFGVRRSKKAYNFEVLPADMFAGKLVIITI
                     IIGLITWTLAGYNGLSWTVVIVLAVVMVYDYVARQTVLGRHIYAVGGNPEAAELSGIS
                     VKRITFVVFGSMGFLCALSGILFASRLQSAIPTAGNLFELDCIAAAYIGGVSAAGGVG
                     TITGSLVGALVYMSLMNGMNLLGTDIALQYVIRGAVLAIAVIFDVATRRRKS"
     gene            complement(1413..1583)
                     /locus_tag="VFL04_04060"
     CDS             complement(1413..1583)
                     /locus_tag="VFL04_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HET7838907.1"
                     /translation="MPRKPEEEEKKRFLLRLDPAVYAALERWAADELRSVNAEIEFLL
                     KEALRRASRLGR"
     gene            complement(1592..2479)
                     /locus_tag="VFL04_04065"
     CDS             complement(1592..2479)
                     /locus_tag="VFL04_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7838908.1"
                     /translation="MKDTIEKKVWRLNGFAALLVTVAAYYLAYRLLRTVALAMGADEA
                     RPAAFWWAVVIIVLASLGMRGFFVIHPNEAKAFTFAGSYVGSLRDQGFHWTNPFAVKK
                     AVMLRVRNFASQVLKVNDAHGNPIEIGAVVVWRVVDSAKALFNVDNYESFVVVQAETA
                     IRFLAGRFPYDAPQDQESLRGSQDEVATELRAQLRSRLSTAGIEIDEARLSHLAYAPE
                     IAQAMLRKQQAGAVIEARRLIVDGAVGMVRMALSELEEQGVVTLDEERKASMVNNLLV
                     ALVSESEAQPVINTGSLYS"
BASE COUNT          415 a          927 c          860 g          477 t
ORIGIN      
        1 gtgggggcga aatacgagat ctacaccatc atgaaccgcc tggtccagca ggggatgagc
       61 atcgtcatga tcagctccga gctgcctgag atcctgggca tgagcgaccg ggtctacgtc
      121 gtggcctcgg gacggatcac cggagagctt ccggccgccg aggcgaccca ggagaccatc
      181 atgcggctgg cgacaagctg agggagggac acaaggtgac cggcactgat cttcaaaagg
      241 tattgcgcaa gaacctgcgc gagtacggca tgtacatcgc gctcgtcatc atcatggcga
      301 tcttctcgat ccagaccaag ggcatgttca tctcgtcccg gaacatagcc aacctcctca
      361 accagacggg ctacatcgcc gtcctcgccg tgggcatgac cctcgtcatc gtgatacggc
      421 acatcgacct ctcggtgggc ttcctcgccg gtttcctcgg cgcggtggcc gccataggcc
      481 tcgcctcgtg gcacctgaac gtctgggtcg tgctggtcct ggtcctcgcc ctcggcgtgg
      541 tggcggggct cgtcacggcc ttccccgtcg cgaggctcgg catcccctcc ttcgtcgcct
      601 cgctcgccgg ctggctcatc taccgcggcg ccctcctcct cgtgacctcg tccaccggca
      661 ccatcatcat cccgaacgac accttcaacg ccatcggcaa cggctacatc cccgacatcc
      721 tgccggccga ctcggccttc ctgcccggcc tccacaagct caccctcgtc ctgggcttgc
      781 ttgccatggt ctggttcgtg atccgcgagt tcggcgtccg caggagcaag aaggcctaca
      841 acttcgaggt cctgcccgcc gacatgttcg ccggcaagct cgtcatcatc accatcatca
      901 tcggcctcat cacctggacc ctggccggct acaacgggct ctcgtggacc gtcgtgatcg
      961 tcctcgccgt ggtcatggtc tacgactacg tcgccaggca gaccgtgctc ggccgccaca
     1021 tctacgccgt cggcggcaac cccgaggccg ccgagctctc gggcatcagc gtcaagcgga
     1081 tcaccttcgt ggtcttcggc tccatgggct tcctctgcgc cctctcgggc atcctcttcg
     1141 cctcgcggct ccagtcggcc atccctacag cgggcaacct cttcgagctc gactgcatcg
     1201 ccgcggcgta catcggcggc gtctcggctg ccggcggggt cgggaccatc accggctcac
     1261 ttgtcggcgc cctcgtctac atgtccctca tgaacgggat gaacctcctc ggcaccgaca
     1321 tcgccctgca gtacgtgatc aggggcgccg tcctcgcgat tgcggtcatc ttcgacgtgg
     1381 cgaccaggcg gaggaagagc taggggggag cttcagcgcc cgagccggga ggcccttcgg
     1441 agggcctcct tgagcaggaa ctcgatctcc gcgttgacgc tccgcagctc gtcggcggcc
     1501 catctttcga gggccgcgta gacggccggg tcgagcctga ggaggaagcg cttcttctcc
     1561 tcctcctcgg gtttccttgg caccgccggc ctcaggagta gaggctgccc gtgttgatga
     1621 cgggctgggc ctcggactcg gagacgaggg cgacgaggag gttgttcacc atcgaggcct
     1681 tcctctcctc gtcgagggtg accacgccct gctcctcgag ctcggagagg gccatcctca
     1741 ccatgcccac ggccccgtcg acgatgaggc ggcgcgcctc gatgacggcc cccgcctgct
     1801 gtttgcgcag catcgcctgg gctatctcgg gggcgtaggc gaggtgggag agtctggcct
     1861 cgtcgatctc gatgccggcc gtggacagcc tcgatcggag ctgggcgcgg agctccgtgg
     1921 ccacctcgtc ctggctgccc ctgagggact cctggtcctg gggggcgtca taggggaagc
     1981 ggccggcgag gaagcggatc gcggtctcgg cctggaccac cacgaagctc tcgtagttgt
     2041 cgacgttgaa gagggccttt gccgagtcca ccacccgcca gaccacgacg gcgccgatct
     2101 cgatggggtt gccgtgggcg tcgttcacct tgaggacctg gctcgcgaag ttcctgacgc
     2161 ggagcatcac cgcctttttc acggcgaagg gattggtcca gtggaagccc tggtcgcgga
     2221 gcgagccgac atagctgccc gcgaaggtga aggccttggc ctcgttgggg tggatcacaa
     2281 agaagccgcg catgcccagc gaggccagga cgatgatcac cacggcccac cagaacgcgg
     2341 ccggtcgggc ctcatccgcg cccatggcga gcgcgacggt cctcagcagc cgataggcga
     2401 gatagtaggc ggcgacggtc acgaggaggg cggcgaagcc gttcagcctc cagaccttct
     2461 tctcgatcgt gtccttcatg gaatcctccc ggagcggcgc gccgctcgat atctagatga
     2521 tatcactgtg ctatcgggag gtcaagctcg aggaggcccg cccgcggctc cgctgcggcc
     2581 cggccaggcc gggcggcccc gagaaggccg cgggccggtc agggcggcgg gctgggcctt
     2641 taaggccggg cggcccgaca aaggcgggcc ggccccctc
//
LOCUS       DASSQV010000237         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_184257, whole genome shotgun sequence.
ACCESSION   DASSQV010000237 DASSQV010000000
VERSION     DASSQV010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184257"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /gene="tpx"
                     /locus_tag="VFL04_04070"
     CDS             complement(<1..477)
                     /gene="tpx"
                     /locus_tag="VFL04_04070"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET7838909.1"
                     /translation="MATVILGGTQVHSAGSLPALGSKAPDFRLTTAELKDVSLKDFGG
                     KVKILNIVVSLDTGVCASSAVKFNREIKKVEGAVVLTISRDLPFAMGRFCKAEGVDVV
                     VPLSELRGRDFGKAYGVEMVDGNLAGLLSRAIVVLDRNDKVVYTQQVPEIGIEPDYE"
     gene            complement(580..1224)
                     /locus_tag="VFL04_04075"
     CDS             complement(580..1224)
                     /locus_tag="VFL04_04075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HET7838910.1"
                     /translation="MKKYIVLAGNIGAGKSTLVGLLAERLGFRPYYEPVAENPYLADF
                     YADMERWAFNSQVFFLTHRVRAHRALMEDPHSVVQDRSLYEDAEVFARNLYEQGSMSR
                     RDWTGYCELYRTLKGLLPPPDLVIYIKASVATLKKRIAMRGREIEAGIEDSYLAGLNR
                     FYEEWIDGFDLAPVLVVPGDRLDFVAESGDLQAIMNVVEDRLRDRQGILFPFGM"
     gene            complement(1353..>2299)
                     /gene="aspS"
                     /locus_tag="VFL04_04080"
     CDS             complement(1353..>2299)
                     /gene="aspS"
                     /locus_tag="VFL04_04080"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HET7838911.1"
                     /translation="DRRFLSLRNPKALGAFRVQATILEAFASTLRAEGFTEVKTSKLV
                     ATGTEGGSGLFEVDYFGQRAFLAQSPQFYKQAMVASGLERVFEIGQAYRAEKHDTPRH
                     LNEYCSLDLELAFIESELDLIELERRLLASIFEAVAAHNAPQLAAWGASVPSPEELAK
                     APVLAHDEAIEIAGREGGSRAFEITPEVERSICSWSERERGSGIVFVNEFPRRQRPFY
                     AFPKGRSTMSFDCLFRGLEVTTGGRRINDYAMLMESLERSGLDESDYADYASIFRYGC
                     PPHGGFAIGLERLTQKIMGFPNVRQASLFPRDRRRLRP"
BASE COUNT          362 a          747 c          807 g          383 t
ORIGIN      
        1 ctcgtagtcg ggctcgatcc cgatctcggg cacctgctgg gtgtagacta ccttgtcgtt
       61 cctgtcgagc accacgatgg cccgcgagag gaggccggcc aggttcccgt ccaccatctc
      121 gaccccgtag gccttcccga agtcccttcc ccgcagctcg gagagcggga ccacgacgtc
      181 caccccctcg gccttgcaga acctgcccat ggcgaagggg aggtcgcggc tgatggtgag
      241 cacgacagcc ccctcgacct tcttgatctc gcggttgaac ttgaccgccg agctcgcgca
      301 gaccccggtg tccaggctca cgacgatgtt caggatcttg accttgcccc cgaagtcctt
      361 gagcgaaacg tccttgagct ccgccgtcgt gaggcggaag tccggagcct tgcttcccag
      421 ggcggggagg ctccccgcgc tgtgcacctg cgtgccgccc aatatcactg tcgccatgga
      481 ttgccccctc ttggtgcgga cgcgcgctgg gcgcgccctc tgctggaagc tactgaggcg
      541 gggcccgagg atcaaggccg aacggagaca ggttgggcgc tacatgccga aggggaagag
      601 gatgccctgg cggtcgcgga gccggtcctc cacgacgttc atgatggcct ggaggtcccc
      661 tgactcggcc acgaagtcca gcctgtcccc cggcaccacg aggacgggcg cgaggtcgaa
      721 gccgtcgatc cactcctcgt agaagcggtt aagcccggcg aggtaggagt cctcgatccc
      781 cgcctcgatc tcccggccgc gcatggcgat gcgcttcttg agcgtggcca ccgaggcttt
      841 gatgtagatc acgaggtcgg gtgggggcag gaggcccttg agggtgcgat agagctcgca
      901 atagcccgtc cagtcccgcc tcgacatcga gccctgctcg tagaggttgc gcgcgaagac
      961 ctcggcgtcc tcgtagagcg agcggtcctg gaccaccgag tggggatcct ccatgagggc
     1021 gcggtgggcg cggaccctgt gggtgaggaa gaagacctgg gagttgaagg cccagcgctc
     1081 catgtcggcg tagaagtccg cgagataggg attctcggct accggctcat agtagggcct
     1141 gaagcccagg cgctccgcga ggagaccgac aagggtggac ttacccgctc cgatgttgcc
     1201 cgcgaggacg atgtatttct tcatgccgcc gactttcggc ctagaataga ccccaggccc
     1261 gcagcgacgc aagccgggcc ggctgaaaaa tgccccccgg gccctgggct tggggccgcc
     1321 ccgcggttgg ccgggccggg gtcggccggg ccctagggcc tcagcctcct cctgtcgcgg
     1381 gggaagaggc tggcctggcg cacgttgggg aagcccatga tcttctgggt gagacgctcg
     1441 agcccgatcg cgaagccgcc gtgtggaggg cagccgtacc tgaagatcga ggcgtagtcg
     1501 gcgtagtcgg actcgtcaag gccgcttcgc tcgaggctct ccatcagcat cgcgtagtcg
     1561 ttgatcctgc ggcccccggt cgtcacctcg agcccgcgga agaggcagtc gaagctcatc
     1621 gtcgagcggc ccttggggaa ggcgtagaag ggcctctgcc tgcgggggaa ctcgttcacg
     1681 aagacgatgc cgctccccct ctcccgctcg gaccaggagc agatcgagcg ctcgacctcg
     1741 ggcgtgatct cgaaggccct gcttccgccc tcccggccgg ctatctcgat ggcctcgtcg
     1801 tgggccagga ccggggcctt cgccagctcc tcggggctcg gcaccgaggc gccccaggcc
     1861 gcgagctggg gcgcgttgtg cgccgccacg gcctcgaaga tggaggcgag gaggcggcgc
     1921 tccagctcga tgaggtcgag ctcgctctcg atgaaggcga gctcgaggtc gagggagcag
     1981 tactcgttga ggtgcctcgg ggtgtcgtgc ttctcggccc ggtaggcctg gccgatctcg
     2041 aagacccgct cgaggcccga ggcgaccatg gcctgcttgt agaactgggg cgactgggcg
     2101 aggaaggcgc gttggccgaa atagtccacc tcgaagaggc ccgagcctcc ctcggtgccc
     2161 gtggccacga gcttggaggt cttgacctcg gtgaagccct cggccctgag ggtggaggcg
     2221 aaggcctcga ggatggtcgc ctggaccctg aaggcgccga gggccttggg gttcctgagg
     2281 ctgaggaagc ggcggtcga
//
LOCUS       DASSQV010000238         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2173052, whole genome shotgun sequence.
ACCESSION   DASSQV010000238 DASSQV010000000
VERSION     DASSQV010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2173052"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFL04_04085"
     CDS             <1..201
                     /locus_tag="VFL04_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7838912.1"
                     /translation="PLEEALRTVSSSPAAMLKLGRKGRIAEGADADLLLVGRESLEVD
                     TVIAGGRVAVARGEALVHGLFE"
     gene            236..2335
                     /locus_tag="VFL04_04090"
     CDS             236..2335
                     /locus_tag="VFL04_04090"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7838913.1"
                     /translation="MKKIALVAGSASTMGELCRQLSAYLGEEAEVAGYAVDEGISSRL
                     EADILVLSSHMLRAELEAAGLIDPSIPTITARRTVNCDHLELVVALPPGTRALFVNDR
                     AESAGECIASLRELGLDYIEYQPYYPGAPDPDPSIRVAITAGELGLVPAGIRQVIDIG
                     VRILDFGTLAELLGHLGILDAKLGQFTQRYLATIVSVAQRLARSTEETGRINGHLAGV
                     IDSLRHGILVYDGSGKVLFCNENLRTMLNLRVEDPAGRSLGAIIRNGSLRDFLECRCG
                     EETAVFRVADRDIVVKRFEAGKEGQTVAAFRADRDAAAENARLAREYRKRGYIAKYDM
                     DDIVGESEPIQRARMIALRLARTDLTILINGESGTGKELFASAIHAASGRSSGPFLAV
                     DLGALSDDLIESELFGYEEGAFTGAKKGGKAGLFELADGGTIFLDEIGHISPKVQSRL
                     LRVLQEKEVLRVGGSEIKRIDVRVIAASNEDLLESARRGGFREDLYFRLKMGLLRIPP
                     LRERASDIPLLIEAFMRSEGQSGVEIASEVLIAFGDYDWPGNVRELRNLLTYMLAVRD
                     GSRVSIADLPDEGFFEGLAPRLGAARAAMARPADQAPRLAAAPPLSAEDGFLLESVGR
                     LEAEGRIAGRELLSSLAAQGGFDLSPARARGRIGRLARLGLLETGRGRHGTRLTPAGR
                     ELLGIRASDQLDPLPRQ"
     gene            2414..>3079
                     /locus_tag="VFL04_04095"
     CDS             2414..>3079
                     /locus_tag="VFL04_04095"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HET7838914.1"
                     /translation="MTGGNLLIVGGAEKPARAEIMRAFVELSAGREGCFLVCPAATED
                     PLGGLERMRSAFAEVGVDPARVRLLELSALVPGWEGGARDPRQAELARTAAGIWFLGG
                     DQNRAVELLLDETGGDTALLAAIREASSVPGGPVLGGTSAGAAIMSHPMIAAGTSFGA
                     LALPRAVGLAEPGSGDERRLLVRPGLGFFPEGIVDQHFDARSRLGRLVEAALVEDGAR
                     RLAF"
BASE COUNT          453 a         1082 c         1099 g          445 t
ORIGIN      
        1 cccctcgagg aggcgctcag gaccgtcagc tcgagtccgg cggccatgct caaactcggc
       61 cgcaagggcc ggatcgccga gggggccgac gccgacctcc tcctggtggg ccgggagagc
      121 ctcgaggtgg acacggtcat cgcgggtggc cgggtcgcgg tcgcgcgggg cgaggccctc
      181 gtccacggcc ttttcgagta aggggatccc tggggcccga atcggagttc cgaaggtgaa
      241 gaagatcgca ttggtggcgg gaagcgccag cacgatgggg gagctgtgcc gccagctcag
      301 cgcgtacctg ggcgaggagg ccgaggtcgc gggctacgcc gtggacgagg gcatctcgtc
      361 ccggctggag gccgacatcc tcgtgctctc gagccacatg ctgcgcgcgg agctcgaggc
      421 ggccggcctc atcgatccct cgatccccac catcacggcc cggcgcacgg tcaactgcga
      481 ccatctcgag ctcgtggtcg ccctgcctcc agggaccagg gcccttttcg tcaacgacag
      541 ggccgaatcg gccggggagt gcatcgcatc cctcagagag ctcggcctcg actacatcga
      601 atatcagccc tactaccccg gggctcctga ccccgacccc tccatccgcg tcgcgatcac
      661 ggccggggag ctcggcctcg tccccgcggg catccgccag gtcatcgaca tcggggtgcg
      721 catcctcgat ttcgggaccc tggccgagct cctcggtcac ctcgggatcc tcgacgcgaa
      781 gctgggccag ttcacccagc gctacctcgc cacgatcgtc tcggttgccc agcgcctcgc
      841 gcgctccacc gaggagacgg gcaggatcaa cggccacctc gccggggtca tcgacagcct
      901 ccgccacggc atcctcgtgt atgacggctc gggcaaggtc ctcttctgca acgagaacct
      961 ccgcacgatg ctgaacctca gggtcgagga ccccgccggc cgcagcctcg gcgcgatcat
     1021 ccgcaacggg agcctgcggg acttcctcga gtgccgctgc ggggaggaga cggccgtctt
     1081 ccgcgtcgcc gaccgggaca tcgtcgtcaa gcgcttcgag gcgggcaagg agggccagac
     1141 cgtcgccgcc ttccgggcgg acagggacgc ggccgccgag aacgcgaggc tggcccgcga
     1201 gtaccgcaag cggggctaca tcgccaagta cgacatggac gacatcgtgg gggagagcga
     1261 accgatccag agggcgagga tgatcgccct gcgtctggcg cggaccgacc tcaccatcct
     1321 catcaacggg gaatcgggga cgggcaagga gctcttcgcc tcggccatcc acgccgcctc
     1381 ggggaggagc tcgggcccct tcctcgccgt cgacctgggg gccctctcgg acgacctcat
     1441 cgagagcgag ctgttcggct acgaggaggg ggccttcacc ggggccaaga aggggggcaa
     1501 ggccggcctc ttcgagctcg ccgacggcgg gacgatcttc ctcgacgaga tcggccacat
     1561 ctccccgaag gtccagagcc ggcttctgcg ggtgctccag gagaaggagg tcctgcgggt
     1621 cggaggctcc gagatcaagc ggatcgacgt ccgcgtcatc gctgcctcga acgaggacct
     1681 gctcgagagc gcccgccgcg gcggtttccg cgaggacctc tacttccgcc tcaagatggg
     1741 gctcctgcgc atccctccgc tgcgggagcg ggcctccgac atccccctgc tcatcgaggc
     1801 cttcatgcgc tccgagggcc agagcggggt ggagatcgcc tccgaggtcc tcatcgcctt
     1861 cggggactat gactggccgg gcaatgtccg cgagctgcgg aacctcctga cctacatgct
     1921 cgccgtgcgg gatgggtcga gggtgagcat cgccgacctc cccgatgagg gcttcttcga
     1981 gggcctggcg ccccgcctgg gcgcggcgag ggctgccatg gcccgccccg ccgaccaggc
     2041 ccctcgtctg gcggcggctc cgcccctgag cgccgaggac ggtttcctcc tcgagtcggt
     2101 ggggaggctc gaggcggagg ggaggatcgc cggcagggag ctcctctcct ccctcgcggc
     2161 ccagggcggt ttcgatctca gcccggcccg ggcgcgcggc aggatcggcc gccttgcccg
     2221 tctgggcctc ctcgagacgg ggaggggccg ccacggcaca aggctgaccc cggcgggcag
     2281 ggagcttctt gggatcaggg cctcggatca gctggacccg ctgcctcgcc aatagcagtc
     2341 caattggctg tatggcgagc caaatgacca aataaagacc aaaaggacgc cgccaaaacg
     2401 ccaaggggga agaatgaccg ggggaaatct gctgattgtg ggcggggccg agaagcccgc
     2461 ccgtgccgag atcatgcgcg ccttcgtgga gctctccgcc gggcgcgagg gctgcttcct
     2521 cgtctgtccc gcggcgaccg aggatcccct ggggggactc gagcggatgc ggtcggcctt
     2581 cgccgaggtc ggggtggacc cggctcgggt gcggctcctc gagctgtcgg ccctggtgcc
     2641 gggatgggag gggggggcca gggacccgcg ccaggccgag ctggcccgga cggccgccgg
     2701 gatctggttc ctcggcggcg accaaaatcg cgccgtcgag ctcctgctcg acgagacggg
     2761 cggggacacg gccctcctcg cggcgatccg cgaagcgagc tctgtccccg gcgggcccgt
     2821 cctgggcggc acgagcgcgg gggcggcgat catgagccac cccatgatcg ccgctgggac
     2881 gagtttcggg gccctcgccc tgccgcgcgc cgtgggcctc gccgagcccg ggagcggcga
     2941 cgagcgccgg ctcctcgtga ggcccggcct cggcttcttc cccgagggca tcgtcgacca
     3001 gcatttcgac gcgcgttccc gcctgggccg cctggtcgag gcggccctcg tcgaggacgg
     3061 ggccaggcgt ctggccttc
//
LOCUS       DASSQV010000239         2942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1302483, whole genome shotgun sequence.
ACCESSION   DASSQV010000239 DASSQV010000000
VERSION     DASSQV010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302483"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFL04_04100"
     CDS             complement(<1..572)
                     /locus_tag="VFL04_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET7838915.1"
                     /translation="MSQLHLLPAIILTPFAGAILLLFVPEGKRNLTRGIALLASGIAL
                     GLSIAAWALYDRAAGGMQFVERFSWVEGFGIHLDFGVNGLSLPLVLLTSIILFAGVFV
                     TWRLETRSKEFFVYTLALVGGVYGVFMSMDLLVLFMFIEIAVIPKYVLISVWGSTRKE
                     YAAMKYTMYLLAGSAISLVAIIAIFAYAHDP"
     gene            complement(569..2479)
                     /gene="nuoL"
                     /locus_tag="VFL04_04105"
     CDS             complement(569..2479)
                     /gene="nuoL"
                     /locus_tag="VFL04_04105"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET7838916.1"
                     /translation="MINAAIALVLAPLVAFLLIVFVTKRSRALSAAVALAGGAASLAL
                     SAWNLVLVLGPEPGSRAFAGAHASVQWLSIPSMGSFRGLEIAMGVLVDPLSATMALIV
                     SLVAFLVLVYSLGYMSHDPGFSRYYAYLSIFAFSMLGLVVSDNYFQTFVFWELVGLCS
                     YLLIGFWFEKPSAADASRKAFVTNRWADFGFMLGVLLLFSLFGTFDFAQLSASIQAAP
                     AGGAAIALVAALILVGPLGKSTQFPFHVWLPDAMEGPTPVSALIHAATMVAAGVYLIA
                     RGFVLFQSVPAVMEAMAYLGGFTALFAASIALVQRDIKRILAYSTLSQLGYMFMALGV
                     GSMSAGMFHLTTHAFFKALLFLGAGSVIHATGEQDIFRMGGLGRRMGITATTFVIGAL
                     ALSGLFPLAGFWSKDEILLASLASGHLGLYVLGLGVAFMTAFYMFRLIFTAFGGRPAE
                     GSHAHESGPAMTLPLIVLAALSLVAGLVGSPWFEEAFGFGFGSFIHSGQAEVARLDLD
                     TALVSTALAAAGIVLAWLIYGRKLIKAERLAAAFAPLHRLLERKFYVDEVYAWVFAKL
                     MLGAGLVFDWIDHALIDGAFDGLGGGARAAGRGLRLWQTGRLQAYAIVIFAAVAVAAL
                     ALSTDLLGGIFR"
     gene            complement(2505..2813)
                     /gene="nuoK"
                     /locus_tag="VFL04_04110"
     CDS             complement(2505..2813)
                     /gene="nuoK"
                     /locus_tag="VFL04_04110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004321.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET7838917.1"
                     /translation="MIGLGHYLTLSALIFCIGFVGAVAKRNAIAVLMGVELMLNAVNL
                     NLVAFDRFGRHAAPLGEAFAIFIIAVAAAEVAVGLAIVLTLYRRRASTSVDDLDSLKC
                     "
BASE COUNT          571 a          871 c         1132 g          368 t
ORIGIN      
        1 gggtcgtggg cgtaggcgaa aatcgcgatg atggcgacga gggagatggc cgagccggcg
       61 aggaggtaca tcgtgtactt catcgcggcg tactccttcc tcgtgcttcc ccagacgctg
      121 atgaggacgt acttcgggat caccgcgatc tcgatgaaca tgaagaggac gagaaggtcc
      181 atcgacatga agaccccgta cacgccgccg accagggcga gggtgtagac gaagaactcc
      241 ttggagcggg tctcgaggcg ccaggtcacg aagaccccgg cgaagaggat gatcgaggtg
      301 aggaggacca ggggcaggct gaggccgttg accccgaagt cgaggtggat gccgaagccc
      361 tcgacccagg agaagcgctc gacgaactgc atgccccccg cggccctgtc gtagagagcc
      421 caggcggcga tggagaggcc gagggcgatg cccgacgcga ggagggcgat gcccctcgtc
      481 aggttccgct tcccctcggg gacgaagagg aggaggatgg ccccggcgaa gggcgtcagg
      541 atgatcgccg ggagcaggtg gagctggctc atctgaagat acctcccagc aggtcggtgg
      601 acagggcgag ggcggccacg gcgaccgctg cgaagatgac gatggcgtag gcctggagcc
      661 tgccggtctg ccagagccgc aggccgcggc cggcggccct ggcgccgccg ccgaggccgt
      721 cgaaggcccc gtcgatgagg gcgtggtcga tccagtcgaa gacgaggccg gcgccgagca
      781 tcagcttcgc gaagacccag gcatagacct cgtcgacgta gaacttgcgc tcgaggaggc
      841 ggtgcagggg ggcgaaggcg gcagcgaggc gctcggcctt gatgagcttc cggccgtaga
      901 tcagccaggc gaggacgatg cccgccgccg cgagggcggt ggagacaagt gccgtgtcaa
      961 ggtcgagacg ggcaacctcg gcctggcccg agtggatgaa ggatccgaag ccgaagccga
     1021 aggcctcctc gaaccagggg ctgccgacga ggcccgccac gagcgagagg gcggcgagga
     1081 cgatgagggg aagggtcatg gcgggacccg actcgtgcgc gtggctcccc tcggccggcc
     1141 tcccgccgaa ggccgtgaag atcagcctga acatgtagaa ggcggtcatg aaggcgacgc
     1201 ccaggccgag gacgtagagc cccaggtgtc ccgaggccag ggaggcgagg aggatctcgt
     1261 ctttgctcca gaagcccgcg agggggaaga gccccgagag ggcgagggcg ccgatcacga
     1321 aggtggtggc ggtgatgccc atcctcctgc ccaggccccc catcctgaag atgtcctgct
     1381 ccccggtcgc gtggatgacg ctgccggccc cgaggaagag gagggccttg aagaaggcgt
     1441 gggtcgtgag gtggaacatg cccgcgctca tgctcccgac cccgagggcc atgaacatgt
     1501 agccgagctg gctcagggtg gagtaggcga ggatgcgctt aatgtcccgc tgcacgaggg
     1561 ctatggaggc ggcgaagagg gcggtgaagc cgccgaggta ggccatcgcc tccatcacgg
     1621 cgggcacgct ctggaagagc acgaagcccc tcgcgatgag gtagacgccc gcggccacca
     1681 tcgtggcagc gtggatgagg gcgctcaccg gggtggggcc ctccatggcg tcgggcagcc
     1741 agacgtggaa ggggaactgg gtggacttgc cgaggggccc gacgaggatc agggcggcga
     1801 ccagggcgat cgccgccccg cccgccggcg cggcctggat ggaggcggag agttgggcga
     1861 aatcgaaggt cccgaagagg gagaagagga ggaggacgcc cagcatgaag ccgaagtcgg
     1921 cccagcggtt cgtcacgaag gccttcctgc tcgcgtcggc cgccgagggc ttctcgaacc
     1981 agaagccgat gaggaggtag gagcagagcc cgacgagctc ccagaacacg aaggtctgga
     2041 agtagttgtc cgagacgacg aggccgagca tggagaaggc gaagatcgag aggtaggcgt
     2101 agtagcgcga gaagccgggg tcgtggctca tgtagccgag cgagtacacc agcacgagga
     2161 aggccaccag gctcacgatg agcgccatcg tcgccgagag ggggtccacg aggacgccca
     2221 tcgcgatctc gagcccgcgg aagctcccca tcgaggggat ggacagccac tgcaccgagg
     2281 cgtgggctcc ggcgaaggct cgggagcccg gctcgggccc gagcaccagg acgaggttcc
     2341 aggccgagag ggccagggag gccgccccgc ccgccagggc gaccgcggcg ctcagggccc
     2401 tcgagcgctt ggtcacgaag acgatgagga ggaaggccac gagcggggcg aggaccaggg
     2461 cgattgctgc gttgatcatg gaagttccca ccttgccgcg gaggctagca tttgaggctg
     2521 tccaggtcgt ccacgctcgt gctcgcgcgg cggcgataga gggtgaggac gatggccagg
     2581 ccgacggcca cctcggcggc ggcgaccgcg atgatgaaga tcgcgaaggc ctcgcccagg
     2641 ggggctgcgt ggcggccgaa gcggtcgaag gccacgaggt tcaggttcac agcgttgagc
     2701 atgagctcga cccccatgag gacggctatg gcgttgcgtt tcgccacggc tcccacgaag
     2761 ccgatgcaga agatgagggc cgagagggtc aggtagtggc cgagtccaat catggggtct
     2821 ccttcgcggt caccgcagtc atggcctcgc ttgcagcagg ggctgttgcc ttcgcagcgc
     2881 cgcccggctt gccgatcgcg atcgcgccca ccagggcggc aaggaggagg aggcccatcg
     2941 cc
//
LOCUS       DASSQV010000240         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2173591, whole genome shotgun sequence.
ACCESSION   DASSQV010000240 DASSQV010000000
VERSION     DASSQV010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2173591"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFL04_04115"
     CDS             complement(<1..784)
                     /locus_tag="VFL04_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7838918.1"
                     /translation="MADRGFPRSTGGGRGAPLDLRADDGLALFPYSTTIDLEEDDLAS
                     PKKKSKEPREYSAPAVDRTLDVLEYMAKSSQPYGATELSRALKIPLNSVFRILKRLTE
                     REYTTQDPISGGYQLGTKVFSLGMSLYTRYELRQRARAHLEWLCRETEETVQMSVPRG
                     EKLLVLDTISPEVEFYLRVVPGSLVYYHPSAFGKAILAFESEERIQEILPPRLPALTP
                     KTILLRTELLKSLEETRKTGLAYDDEEYTTGILCIGAPVFDVT"
     gene            complement(774..2030)
                     /locus_tag="VFL04_04120"
     CDS             complement(774..2030)
                     /locus_tag="VFL04_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET7838919.1"
                     /translation="MNGKERIRLALDHREADRVPVDFGGCAQTTIHVSVIAALREHFG
                     LPKEPVIVEEPYTMMGRLDEGLKRAMGVDVDALNPLYSFFGIPRTGWKEYRMEDGLEV
                     LVPGDFHISRDAAGDVFTYPQGDTSVPPSARMPKGGYYFDALMRQEPIVEEELRAEDN
                     LEEFGPITEAELDFLADGLAAQAGNGRASLGVVPGAGLGDIACVPAPWMKHPKGIRDI
                     EEWYISTVARTDLLHEIFERQTDIAIQNLARIHERIGDGIEVAWVCGADFGTQTSTFC
                     SPATFNELYKPYYRKVNDWIHRHTAWRTFKHCCGAIETFMEAFIESGFDVMNPVQWTA
                     AGMDPKRLKERYGSRIVFWGGGVDTQRTLPFGSPEQVRAEVLGVCETFAPGGGFVFNT
                     IHNIQANTPVANVVAMLDALDEFNGR"
     gene            2252..>3004
                     /locus_tag="VFL04_04125"
     CDS             2252..>3004
                     /locus_tag="VFL04_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7838920.1"
                     /translation="MTKVLAILLVLCIGVGALSAQVQNKKFVGPADQTYYMCTFVSGV
                     EYWVAAFEGFKDAAKQMGVKAVYQGTTEYDGPKQVTAFEQIMTKKPAGIALSPVDDAA
                     FIEPVKAAMAAKIPVVTFASDTSTAKVGFVTSSNEKEGAFAARAMAKALGGKGKVMVT
                     RNTQTNHQIRVNTFIKTLKDEFPNIQVVAEYMSQQDGAKTYAAIMSVAQKNPDLGGIF
                     SPEGPSGRAAAQASVELGGKIKVLCCDMDAAIL"
BASE COUNT          511 a          970 c          974 g          549 t
ORIGIN      
        1 cggtgacgtc gaagaccggg gccccgatgc agaggatccc cgtcgtgtac tcctcgtcgt
       61 cgtaggcgag gcccgtcttc ctcgtctcct cgaggctctt gaggagctcg gtgcggagga
      121 ggatggtctt gggcgtgagg gccgggagcc ggggcggcag gatttcctgg atcctctcct
      181 cgctctcgaa ggcgaggatg gccttgccga aggcgctcgg atggtaatag acgaggctcc
      241 cgggcacgac gcggaggtag aactcgacct cggggctgat cgtgtcgagc acgaggagct
      301 tttccccccg cggcaccgac atctggacgg tctcctcggt ctcgcggcag agccactcga
      361 ggtgggccct cgccctttgg cggagctcgt agcgcgtgta gaggctcatg cccagggaga
      421 agaccttggt gccgagctga tagccgcccg agatcgggtc ctgggtcgtg tattcccgct
      481 cggtgaggcg cttaaggatg cggaagaccg agttgagggg gatcttgagg gcccgggaga
      541 gctcggtggc cccgtagggc tgggagctct tggccatgta ctcgaggacg tcgagggtcc
      601 ggtccacggc gggcgccgag tattcccggg gttccttgct cttctttttg gggctggcca
      661 ggtcgtcctc ctcgaggtcg atcgttgtgc tgtatggaaa taaggctagc ccatcgtcag
      721 cgcgcaggtc aagtggcgct cccctcccgc cccccgtgga ccgggggaag cccctatcgg
      781 ccattgaact cgtccaaggc gtccagcatg gccacgacgt tggcgacggg ggtgttcgcc
      841 tggatgttgt ggatcgtgtt gaagacaaag ccgccccccg gggcgaaggt ctcgcagacg
      901 ccgagcacct cggccctgac ctgctcgggg ctgccgaagg ggagggtcct ctgggtgtcg
      961 accccgccgc cccagaacac gatccgcgag ccgtagcgct ccttgaggcg cttcgggtcc
     1021 atgcccgccg cggtccattg cacggggttc atgacgtcga aaccgctctc gatgaaggcc
     1081 tccatgaagg tctcgatggc cccgcagcag tgcttgaagg tcctccaggc ggtgtgccgg
     1141 tggatccagt cgttgacctt gcggtagtag ggcttgtaga gctcgttgaa ggtggccggg
     1201 gagcagaagg tcgaggtctg ggtgccgaag tcggccccgc agacccaggc cacctcgatc
     1261 ccgtccccga tccgctcgtg gatcctcgcc aggttttgga tggcgatgtc ggtctggcgc
     1321 tcgaagatct cgtggaggag atcggtgcgg gccaccgtgg agatgtacca ttcctcgatg
     1381 tccctgattc ccttgggatg cttcatccag ggggcgggca cgcaggctat gtcccccagg
     1441 ccggcccccg gcaccacgcc gaggctggct cggccgtttc cggcctgggc cgcgaggcca
     1501 tcagcaagga agtcgagctc ggcctcggtg atcggaccga actcctcgag gttgtcctcg
     1561 gccctgagct cctcctcgac aatgggctcc tgcctcatga gggcgtcgaa gtagtagccg
     1621 cccttgggca tccgggcgct cgggggtacc gaggtgtcgc cctgggggta ggtgaagacg
     1681 tcgccggcgg cgtcgcggct tatatggaag tctccgggca cgaggacctc gagcccgtcc
     1741 tccatgcggt actccttcca gccggttcgg gggatgccaa agaaggaata gagggggttg
     1801 agggcgtcga cgtccacccc catggcccgt ttcaggccct cgtccagacg gcccatcatc
     1861 gtgtagggct cctcgacgat caccggctcc ttggggagtc cgaagtgctc gcggagggcg
     1921 gcgatcacgc tcacgtggat cgtcgtctgg gcgcagccgc cgaagtccac cgggaccctg
     1981 tcggcctccc tgtggtcgag ggcgagacgt atcctttcct ttccgttcat ggatcgatct
     2041 cctttttgat gggtgggatt cgttctgggc cagccagcct aggcgaatta taccggatta
     2101 ccccgttcgg tcaacatata ttcgttatat caaatctaac gactaccagt aaaatattcg
     2161 ttgacaaaac tgggaacctg gcccacactg cccccaagag ctggacgcac agattcgccc
     2221 agccatcaac aggaggttta ttgtggccaa gatgacgaaa gttctcgcga ttctcctcgt
     2281 gctctgcata ggagtcgggg ccctgtccgc ccaggtccag aacaagaagt tcgtgggccc
     2341 ggccgaccag acctactaca tgtgcacctt cgtctccggc gtcgagtact gggtcgccgc
     2401 cttcgagggc ttcaaggacg cggccaaaca gatgggcgtg aaggccgtct accagggcac
     2461 caccgaatac gacggcccca agcaggtcac cgccttcgag cagatcatga ccaagaagcc
     2521 ggccggcatc gccctctccc ccgtcgacga cgcggccttc atcgagccgg tcaaggcggc
     2581 catggccgcc aagatccccg tcgtcacctt cgccagcgac accagcaccg ccaaggtcgg
     2641 cttcgtcacc tcctccaatg agaaggaagg cgccttcgcc gcccgcgcga tggccaaggc
     2701 cctcggcggc aagggcaagg tcatggtcac ccgcaacacc cagaccaacc accagatccg
     2761 cgtcaacacc ttcatcaaga ccctcaagga cgagttcccg aacatccagg tcgtggccga
     2821 atacatgtcc cagcaggacg gggccaagac ctacgccgcc atcatgagcg tcgcgcagaa
     2881 gaaccccgac ctcggcggca tcttctcccc cgaaggcccc tccggccgcg cggccgccca
     2941 ggcctccgtc gagctcggcg ggaagatcaa ggtcctctgc tgcgacatgg acgccgccat
     3001 cctc
//
LOCUS       DASSQV010000241         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_556776, whole genome shotgun sequence.
ACCESSION   DASSQV010000241 DASSQV010000000
VERSION     DASSQV010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556776"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFL04_04130"
     CDS             complement(<1..287)
                     /locus_tag="VFL04_04130"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7838921.1"
                     /translation="MLVFILRRLTQSVVVIFLVSILVFIGVFSIGNPADILIPAEASQ
                     MEREVAIRSLGLDKPMYEQYFICLANATKGDLGRSFVFNQPTLSLIAARMPA"
     gene            complement(449..1090)
                     /locus_tag="VFL04_04135"
     CDS             complement(449..1090)
                     /locus_tag="VFL04_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7838922.1"
                     /translation="MLDILIADDHFLVRKGLRQLLEENLPASRIDEAADGPAVLELAR
                     KGRYDLMILDFAMPGKDGLDLIRDLKDIDPSTHVLVLSILPEEQYAVRAFRLGASGCV
                     NKAIDPSELLDAVKTVIAGHRYLSRKAQDLLLGEIDYEGDLHERLSDREFQLLRLLAS
                     GKSVGEAAGLLSLSVKTVSTYRTRLLEKMGMENNAQLTNYAFKHGLVGGPQSL"
     gene            complement(1091..>2415)
                     /locus_tag="VFL04_04140"
     CDS             complement(1091..>2415)
                     /locus_tag="VFL04_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HET7838923.1"
                     /translation="VEDREGVLWILSSRGLTSYDHGVGAFFLFGAEDGLAAGSLATGA
                     ALLAGNGELWIGGREGLSRLDPSKASRYAPQPDVVITGLEQAESGPGMRAAPLPPGEA
                     QVILGHDNPGLVFRIAIIDYVAPSRNVYAMRLEGRQSGWTSLASQNSGYIAPLAPGRY
                     TLRVKGSNGNGVWNDYGASLAILVRPPFWSTWWFRAIAAGLLAGLLLLLVAARLGALR
                     KRNALLAGFARHVEEAREEERKIAARDVHDEIGQFLMVLNFQAFWLASHPDAAEGERA
                     TRLKDMQGVILDAMASVKAVATRLRPGALDALDFAGALRWYAKSIERLGSLRLVLELE
                     EPPAPVSGDKATALFRILQEMLSNVMRHSGAVSAKVRLASESGYLVLEVIDDGRGIEE
                     GRAEAADSFGIIGMRERCRAFGGSLSIKALSGGGTLVRARLPMAEGRG"
BASE COUNT          405 a          784 c          829 g          397 t
ORIGIN      
        1 gccggcatgc gcgcggcgat gagggagagg gtgggctggt tgaagacgaa ggaccggccg
       61 aggtcgcctt tcgtggcgtt cgccagacag atgaagtact gctcgtacat cggcttgtcg
      121 aggcccaggg acctgatcgc cacctccctt tccatctgcg aggcctcggc ggggatgagg
      181 atgtcggcag gattcccgat ggagaagacg ccgatgaaga ccaggatcga gacaaggaag
      241 atgacgacca cgctctgggt cagccgcctg aggatgaaca ctagcactct tcgcacccct
      301 gctgcctgct ggatatcgcc atggcgcccg ccccgttccg aggcccctcg ggctcatggc
      361 catgccgcag ccgaggtggc ttggagtctg gcccgggaag ggccccgggt atgtaggagg
      421 aggtcatttc attgcgtagg acaaagccct acagggactg gggaccgccg acgaggccgt
      481 gcttgaaggc gtagttggtg agctgggcgt tgttctccat gcccatcttc tcgagcagcc
      541 tggtcctgta ggtgctgact gtcttcacgc tgagggagag gaggccggcg gcctcgccga
      601 cgctcttgcc cgaggcgagg aggcgcagga gctggaactc cctgtcggac agcctctcgt
      661 ggagatcgcc ctcgtagtcg atctcgccca gaaggaggtc ctgggccttg cgcgacaggt
      721 agcggtgtcc cgcgatcacg gtcttcacgg catcgaggag ctcgctggga tcgatggcct
      781 tgttgacgca gcccgaggcc ccgaggcgga aggccctgac cgcgtactgc tcctcgggga
      841 ggatggagag gaccaggaca tgggtggagg ggtcgatgtc cttcaggtcc ctgatgaggt
      901 cgagcccatc cttgcccggc atggcgaagt cgaggatcat gaggtcgtag cgccccttcc
      961 tcgcgagctc gagcaccgcg gggccgtcgg cagcctcgtc gatccgggag gcagggaggt
     1021 tctcctccaa aagctgcctg aggcccttgc gtacaaggaa gtggtcgtcg gcgatcagaa
     1081 tgtcgagcat ctagcccctc ccctctgcca tcggcagcct ggcgcggacg agggtcccgc
     1141 ccccgctaag ggccttgatg ctcaggcttc cgccgaaggc cctgcagcgc tccctcatgc
     1201 cgatgatgcc gaaggagtcg gccgcctcgg cccgtccctc ctcgatcccc ctgccgtcgt
     1261 cgatcacctc gagcacgagg taccccgatt cgctcgcgag gcgcaccttc gccgagaccg
     1321 ctccggagtg ccgcatgacg ttgctgagca tctcctgcag gatccggaac agggcggtcg
     1381 ccttatcgcc ggagacggga gctgggggct cctcgagctc gagcacgagt ctgagactgc
     1441 ccagcctctc gatgcttttc gcgtaccagc gaagggcacc ggcgaaatca agggcgtcaa
     1501 gggcccctgg gcggagccgc gtggcgacgg ccttcaccga ggccatcgcg tccaggatca
     1561 cgccctgcat gtccttgaga cgcgtggccc tttcgccctc cgccgcatcg ggatgggagg
     1621 cgagccagaa agcctggaaa ttgaggacca tcaggaactg cccgatctcg tcatggacat
     1681 cccgggcggc gatcttcctt tcctcctccc tcgcctcctc gacatggcgg gcgaagccgg
     1741 ccagcagggc attcctcttt ctcagggcgc cgagccgcgc ggcgaccagg agcaggagca
     1801 agcccgcaag gaggcccgcg gcgatggccc tgaaccacca ggtcgaccag aaggggggcc
     1861 tcaccaggat cgccagcgag gccccatagt cgttccagac cccgttgccg ttcgagccct
     1921 tgaccctgag ggtgtagcgc ccgggcgcca gcggggctat gtagcccgag ttctgcgagg
     1981 ccagggaggt ccagccggat tgcctgccct cgagcctcat ggcgtagacg ttgcgcgatg
     2041 gagcgacgta gtcgatgatc gcgatgcgga agaccaggcc ggggttgtcg tgacccagaa
     2101 tgacttgggc ttcgccgggg ggcaatgggg cagcgcgcat accaggcccg gactcggcct
     2161 gctcgaggcc ggtgatgacg acgtcgggct ggggagcgta gcgggaggcc ttgctcgggt
     2221 cgagacggga gaggccctcc ctgcctccga tccagagttc cccgttcccg gccaggaggg
     2281 ccgcccccgt ggccagactg ccggcggcca acccgtcctc ggcgccaaac aggaagaagg
     2341 cgcccacccc gtgatcgtag ctcgtgagtc cgcgcgagga gaggatccag agcacgccct
     2401 ccctgtcctc cacga
//
LOCUS       DASSQV010000242         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_59328, whole genome shotgun sequence.
ACCESSION   DASSQV010000242 DASSQV010000000
VERSION     DASSQV010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59328"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VFL04_04145"
     CDS             complement(<1..874)
                     /locus_tag="VFL04_04145"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET7838924.1"
                     /translation="MSEYAIPLARTRRPEPRGGRASELGSTRSSPGPAHRAQRRAGRS
                     RGSSLSERILVVLIAAASVAALATAVLILAPSLLRISRYEITGALSMKREEVLSAALI
                     HGSEYFLAVDPERIRANLLADPRILDAQVHRRFPNALAISVRERVPVVAVLAELGGRS
                     TAISIDAQGVAFALADPAGLGSLPVLSGLRFEDFRLGTRLPESLTPILSALGEIELHD
                     AALLSAFSEIRIVKPSYGEPELLLYPIQHRIPVRAGAALNAQSLRSMILVLDVLGSRP
                     FAGLVEEIDFRTGTV"
     gene            complement(867..>2000)
                     /locus_tag="VFL04_04150"
     CDS             complement(867..>2000)
                     /locus_tag="VFL04_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HET7838925.1"
                     /translation="LAATCLLAGVGLASLWSASAGYALSIGKGAHYFVLRQALYYLPA
                     LAAFLVCALVPLERLRAKVGIITIVGLGLLFLPFIPGLGENRNGASRWIDLGFSTFQP
                     SELWKPISVLYLAHIMDRRGKDLSEGAGVLLPPLMLTGFACLVVYMQNDFSTAVVVGA
                     AAVIVFWAAGAPYSFFLGLGAAVTPLAALSVLTSDFRLRRILAFLFPAYEPHGQGYQV
                     LGSIRAIRAGGLVGKGIGLGTLKLASVPEVQSDFVFAAWTEETGFLGVLAFFAIWGLL
                     AWRAFRNAFAEEDAFRSYLGFGLASLLVMEVLVNVAVAAGVVPATGLALPFFSAGGSS
                     LIAAAIICGLLYNLSRGRAEAEAARPEDRPMARAYGVGEDANV"
BASE COUNT          354 a          623 c          788 g          235 t
ORIGIN      
        1 caacggtgcc cgtgcggaaa tcgatctcct cgacgaggcc cgcgaaggga cgggagccca
       61 ggacgtcgag gacgagtatc atagagcgca gggattgggc gttcaaggcg gcccccgcgc
      121 gcacggggat gcggtgctgg atcggataga ggaggagctc gggctcgccg tagctcggct
      181 tcacgatcct gatctcggag aaggccgaga gcagtgccgc gtcatggagc tcgatctcgc
      241 cgagggccga gagtatcggc gtcaggcttt cggggagcct cgtgccgagg cggaagtcct
      301 cgaagcggag ccccgagagc acgggcagcg agccgaggcc cgcggggtcg gcgagggcga
      361 aggcgacgcc ctgggcgtcg atcgagatgg cggtggaccg cccgcccagc tcggcgagga
      421 cggcgaccac gggcacccgc tccctgacgc tgatcgccag ggcgttcggg aagcgtctgt
      481 ggacctgggc gtcgaggatg cggggatcgg cgaggaggtt ggccctgatc cgctcggggt
      541 cgacggcgag gaaatactcg gagccgtgga tcagggcggc cgagagcacc tcctcgcgct
      601 tcatggacag ggcccccgtg atctcgtagc gcgagatcct gaggagggat ggggccagga
      661 tgaggacggc cgtggcgagg gccgcgaccg acgccgcggc gatcaggacc acgaggatcc
      721 tctcgctcag gctcgagccg cgggaccgcc ccgcccttcg ctgcgcgcga tgcgccggac
      781 cgggggagct ccgcgtcgag ccaagctcgg aagcgcgccc gccgcggggc tcggggcgtc
      841 ttgtcctcgc caggggaatc gcgtactcag acattggcgt cctcccccac cccataggcc
      901 ctcgccatgg ggcggtcctc gggccgggcg gcctcggcct cggcgcggcc ccgcgacagg
      961 ttgtacagga ggccgcagat gatcgcggcc gcgatgaggc tcgagccccc cgcggagaag
     1021 aagggcaggg ccagaccggt cgccgggacc acgcccgcgg cgacggcgac gttgacgagg
     1081 acctccatca caaggaggct cgcgaggccg aagcccaggt atgacctgaa ggcgtcctcc
     1141 tcggcgaagg cgttcctgaa ggcgcgccag gcgaggaggc cccagatggc gaagaaggcc
     1201 agcacgccga ggaagccggt ctcctcggtc caggcggcga agacgaagtc ggactggacc
     1261 tcggggaccg aggccagctt cagggtcccg agcccaatcc ccttgcccac gaggccgccc
     1321 gccctgatgg ccctgatcga gccgaggacc tggtagccct ggccgtgggg ctcgtaggct
     1381 gggaagagga aggcgaggat gcgcctgagg cggaagtccg aggtgaggac cgagagggcg
     1441 gccagggggg tcacggcggc gccgaggccc aggaagaagc tgtagggcgc tccggccgcc
     1501 cagaacacga tcacggccgc cgccccgacg accacggccg tggagaagtc gttctgcatg
     1561 tacacgacga ggcaggcgaa gccggtgagc atgaggggcg gcaggaggac gcccgctccc
     1621 tcggacaggt ccttgccgcg cctgtccatg atgtgggcga ggtagagcac cgatatgggt
     1681 ttccagagct ctgagggctg gaaggtggag aagccgaggt cgatccagcg ggaggctccg
     1741 ttacggttct ccccgaggcc agggatgaag ggcaggaaga ggaggccgag gccgacgatg
     1801 gtgatgatgc ccaccttcgc gcgcagccgt tccaggggca cgagggcgca gacgaggaag
     1861 gcggcgaggg cggggaggta gtacagggcc tgcctgagga cgaagtagtg ggcgcccttg
     1921 ccgatcgaga gcgcataacc cgcagaggcc gaccagagcg aggcgaggcc caccccggcg
     1981 aggaggcagg tcgcggccag
//
LOCUS       DASSQV010000243         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2174030, whole genome shotgun sequence.
ACCESSION   DASSQV010000243 DASSQV010000000
VERSION     DASSQV010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174030"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /gene="lysS"
                     /locus_tag="VFL04_04155"
     CDS             complement(<1..1355)
                     /gene="lysS"
                     /locus_tag="VFL04_04155"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET7838926.1"
                     /translation="MEQPNRAGHWADATADQIIAEKGDKELYTCASGITPSGTVHVGN
                     FREMISVDLVVRALRDKGKKVRFIYSWDDYDVFRKVPVNMPQQEMLAAHLRFPITLVP
                     DPWGRDSSYARHHEVDVESVLPTVGIFPEFISQTERYRASRYAEGMKRAMEQREVLKR
                     ILDKYRDEDHKIQGEWWPVSVFCDNCNRDTTQIEGWDGDWGLSYRCETCGHAEKLDLR
                     KAKGAKLFWRVDWPMRWAFEGVDFEPAGKDHHSQGGSFDTAKHVVEDVYGAKPPVSFR
                     YDFIGIKGSPGKMSSSKGKVVDLPDILRVYQAEVVRYLFAGTRPNTEFVISFDLDVIK
                     IYEDYDKTERIYWGVEQAKDEDTRLKERRIYELSQVGAVPERMPYQLAFRHLCSLLLI
                     HLGDVDKVLASLPGLEEHQVPKLRERAACAWYWVNECSPEDFRFTVRNPGERIELSEN
                     EA"
     gene            complement(1396..>2184)
                     /locus_tag="VFL04_04160"
     CDS             complement(1396..>2184)
                     /locus_tag="VFL04_04160"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET7838927.1"
                     /translation="DGVSYDHVFHYQLYGARTLRAFWSPSVSLEMFKKTVPIDYAELQ
                     DRCVKIKAILDKAVSVRVTNPNGTDLRIGLAGRSAKSDDGDYSVAGSGGNLPAGEVFI
                     SPELGTAAGSLVFDGSIASTTGDILIREPIRCAVEGGFVTRVEGGSEARELQKALEAG
                     SEGAMRMASNGELSADKAETYARNARALGELGIGLNPAAEVVGNMLEDEKAFRTCHIA
                     IGSNYDEDGPALIHLDGLISRPTIVAIMADGKETVIERGGELLV"
BASE COUNT          321 a          710 c          745 g          408 t
ORIGIN      
        1 gcctcgttct cgctcagctc gatcctctct cccgggttcc tcaccgtgaa gcggaaatcc
       61 tcgggggagc actcgttgac ccaataccag gcgcaggccg ccctctccct gagcttgggc
      121 acctggtgct cctcgaggcc agggagggag gcgaggacct tgtccacgtc gcccaggtgg
      181 atcaacagga ggctgcagag gtggcggaag gccagctggt agggcatccg ctccgggacg
      241 gcgcccacct gggagagctc gtagatccgc ctctccttga gccgggtgtc ctcgtccttg
      301 gcctgctcaa cgccccagta gatcctctcg gtcttgtcgt agtcctcgta gatcttgatg
      361 acgtcgaggt cgaagctgat gacgaactcg gtgttgggcc tcgtgcccgc gaagaggtag
      421 cggaccacct cggcctggta gaccctgagg atgtcgggca ggtcgacgac cttgcccttg
      481 ctcgaggaca tcttgcccgg cgagcccttg atcccgatga agtcgtagcg gaagctcacc
      541 gggggcttgg cgccgtagac gtcctcgacg acgtgcttcg cggtgtcgaa ggagccgccc
      601 tgggagtggt ggtccttgcc cgcgggctcg aagtcgacgc cctcgaaggc ccagcgcatg
      661 ggccagtcga ccctccagaa gagcttggcc cccttggcct tcctgaggtc gagcttctcg
      721 gcgtggccgc aggtctcgca gcggtaggag aggccccagt ccccgtccca gccctcgatc
      781 tgggtggtgt cgcggttgca gttgtcgcag aagaccgaga ccggccacca ctcgccctgg
      841 atcttgtggt cctcgtcgcg gtacttgtcg aggatgcgct tcaggacctc gcgctgctcc
      901 atggcccgct tcatgccctc ggcgtaacgc gatgcgcgat accgctcagt ctgggatatg
      961 aactcgggga agatgcccac cgtcgggagc accgactcga cgtcgacctc gtggtggcgg
     1021 gcgtagctcg agtcccggcc ccaggggtcg ggcacgaggg tgatggggaa gcggaggtgg
     1081 gcggccagca tctcctgctg gggcatgttc accggcacct tgcggaagac gtcgtagtcg
     1141 tcccagctgt atatgaagcg gaccttcttg cctttgtcgc gcagggcccg cacgacgaga
     1201 tcgaccgaga tcatctcgcg gaaattgccc acgtggaccg tgcccgaggg ggtgatcccc
     1261 gaggcgcagg tgtagagttc cttgtcgccc ttctccgcga tgatctggtc ggcagtcgcg
     1321 tctgcccagt gccctgccct gttcggttgt tccatgcgga ggagtatagc ctgtttgccg
     1381 aaacagcgac aacgcctaga ccaacagctc cccgccgcgc tcgatcacgg tctccttgcc
     1441 gtcggccatg atggccacga tggtgggccg ggagatgagg ccgtcgaggt ggatcagggc
     1501 ggggccgtcc tcgtcgtagt tggagccgat ggcgatgtgg caggtcctga aggccttctc
     1561 gtcctcgagc atgttgccga cgacctcggc cgccgggttg aggccgatgc cgagctcgcc
     1621 caaggccctg gcgttcctgg cgtaggtctc ggccttgtcg gccgagagct cgccgttcga
     1681 ggccatccgc atcgcgccct ccgagccggc ctcgagggcc ttctgcagct cgcgcgcctc
     1741 gctcccgccc tcgaccctgg tcacgaagcc cccctccacg gcgcacctga tgggctcgcg
     1801 gatgaggatg tcgcccgtgg tgctcgctat ggagccgtcg aagacgaggc tgcccgcggc
     1861 ggtgccgagc tcgggggaga tgaagacctc gcccgccggg aggttgccgc ccgagcctgc
     1921 cacggagtag tcgccgtcgt cgctcttcgc cgaccggccc gcgaggccga tcctgaggtc
     1981 ggtgccgttg gggttcgtga cccgcacgga gaccgccttg tccaggatgg ccttgatctt
     2041 gacgcagcgg tcctggagct cggcgtagtc gatggggacg gtcttcttga acatctcgag
     2101 cgagactgag ggcgaccaga aggcccgcag ggtcctcgcg ccgtagagct ggtagtggaa
     2161 cacgtggtcg taggagaccc cgtc
//
LOCUS       DASSQV010000244         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_682143, whole genome shotgun sequence.
ACCESSION   DASSQV010000244 DASSQV010000000
VERSION     DASSQV010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682143"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2148
                     /locus_tag="VFL04_04165"
     CDS             <1..2148
                     /locus_tag="VFL04_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7838928.1"
                     /translation="ASAGPADGGRQVHALRVIARGSHITIIVDDAWAGEAVDDSFRLG
                     HIAFAAQNYGPGGGDAGRTAGVPGSSTRAVLETCMLDSRPLSVETWYYRWNYYILPEP
                     AARRRLAETFFAMGEHLAAAIQLRKLERRRALDADELFLKAEISLRLEDHEEAAAALD
                     ACLALDPARRDAAEEKANLLYLSGDYAGLREALAPLLEGQPDNARLQGLLGHARFALG
                     DFEGAAAAYRSAAELDAEQPLFRMNEARAWDQAKRRTEAIEAYLAAARLFFRDEADDD
                     LSLALGRLIALKARGTEVRELRAKVLFRQGRREEAAKLLAELVAKGSTDSAIHYLHGI
                     ILAGKGDRARALERFTAALELEPDYPLYAFRYAESLFLLEQAAAGGKAGPSGHECPDR
                     AKVDAAIERALELAPADGWVLNLAGQAALERGEAEAARAHLEAARAALPGAPEPAINL
                     AELETRSGRLEEALAILAPFPENAACRNQAGNVLSRQAGLGEEGLQAAAREYERACAL
                     DPTRAEYQANLAAVYLEQERYSDAEARIRAALDLGSDARTCLLAGNLALVYGDRPRAE
                     ASYRVGLELSPSDPALLMAMGRHWVAGKQTRKAEEIRKRLEAVDAERAARLGAEIEDM
                     STEGLSCASCGRSWRVPRKLPVQSEASIRAMPPDESPAGACPRCGKIYCIACRKGLLV
                     DNRFTCPVCGESLKLSDDRLRYLVREHLRTAAT"
     gene            2225..>2589
                     /locus_tag="VFL04_04170"
     CDS             2225..>2589
                     /locus_tag="VFL04_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HET7838929.1"
                     /translation="MKSISLFLLLTFAVLAAKAQDITTADSFFAQMADRYAQIKDYEG
                     HLAITLNADKAPSMTGSVSFKAPSLLRIDFIQPPDQVIAFDGTTLTVYIPSYRAVLSQ
                     TAGDKPGGAGGASLASSEGL"
BASE COUNT          410 a          993 c          839 g          347 t
ORIGIN      
        1 gcttccgccg ggcccgccga cggggggagg caggtccacg ccctccgggt catcgcccgc
       61 ggcagccaca tcaccatcat cgtcgacgac gcatgggccg gagaggccgt ggacgacagc
      121 ttcaggctcg gccacatcgc cttcgcagcc cagaactatg ggccaggcgg cggggatgcg
      181 ggccgcacgg ccggagtccc cggctcatcc acgagggccg tcctcgagac ctgcatgctc
      241 gactcgaggc cgctttcggt cgagacctgg tactaccgct ggaactacta catcctgccc
      301 gagcccgccg ccagaaggag gctcgccgag accttcttcg ccatgggcga gcacctggct
      361 gccgccatcc agctgcgcaa gctcgagcgg aggcgggccc tggacgccga cgagctcttc
      421 ctgaaggccg agatcagcct ccgcctcgag gaccacgagg aggccgccgc agccctcgac
      481 gcctgtctcg ccctcgatcc ggcccggagg gacgccgccg aggagaaggc caacctcctc
      541 tacctcagcg gtgactacgc cgggctccgc gaggccctcg cccccctcct tgagggccag
      601 cccgacaacg cccggctcca gggcctcctc ggccacgccc gtttcgccct gggcgacttc
      661 gagggggcgg ctgcggccta ccgctcggcc gccgagctcg acgccgagca gccgctgttc
      721 cggatgaacg aggcgagggc ctgggaccag gccaaacgcc ggaccgaggc gatcgaggcc
      781 tatctggccg ccgcccggct cttcttccgc gatgaggccg acgacgacct ctccctcgcc
      841 ctcggccgcc tcatcgccct caaggcgagg ggcaccgagg tcagggagct ccgcgcgaag
      901 gtcctcttcc gccagggaag gcgggaggag gctgccaagc tcctggccga gctcgtggcc
      961 aagggctcga ccgactccgc gatccactac ctccacggga tcatcctcgc tggcaagggg
     1021 gacagggctc gggccctcga gcgcttcacc gcggccctcg agctcgagcc cgactacccc
     1081 ctctacgcct tccgctacgc cgaaagcctc ttcctcctcg aacaggccgc ggcgggcggc
     1141 aaggctggcc cttcgggcca tgagtgcccg gaccgggcca aggtcgacgc ggcgatagag
     1201 cgcgccctcg agctagcccc cgccgacggc tgggtcctga acctcgccgg ccaggcagcc
     1261 ctcgagcgcg gcgaggccga ggccgcccgc gcccacctcg aggcggccag ggccgcgctt
     1321 cccggcgccc ccgagcccgc gatcaacttg gccgagctcg agacgcgctc gggcaggctc
     1381 gaggaggccc ttgccatact cgcgcccttc cccgagaacg ccgcctgcag gaaccaggcc
     1441 gggaacgtcc tttcaaggca ggcggggctg ggcgaggagg ggctccaggc cgccgcgcgc
     1501 gagtacgagc gggcctgcgc cctcgatccg acccgggccg agtaccaggc caacctggcg
     1561 gcggtctatc tcgagcagga gcgctactcg gacgccgagg ccaggatcag ggcggccctc
     1621 gacctgggaa gcgacgcgag gacctgtctc ctcgccggca acctcgccct cgtctatggc
     1681 gacaggccca gggccgaggc ctcctaccgc gtcggcctcg agctctcgcc ctccgacccc
     1741 gccctcctca tggccatggg ccggcactgg gtagcgggca agcagaccag gaaggccgag
     1801 gagatcagga agcggctcga ggccgtggac gccgaaaggg cggcccggct cggcgccgag
     1861 atcgaggaca tgtccaccga gggcctctcc tgcgcgagct gcggccgctc ctggcgggtg
     1921 ccacgcaagc tcccggtcca gagcgaggcc agcatcaggg ccatgccgcc cgacgagtcc
     1981 cccgccggcg cctgcccccg ctgcggcaag atctactgca tcgcctgccg gaaggggctc
     2041 ctggtggaca accgtttcac ctgcccggtc tgcggggaaa gcctcaagct ctccgacgac
     2101 cggctccgct atctggtgcg cgagcatctg aggacggcgg ccacctaggc gcgccttgtc
     2161 ttttccgcta atacgggcta caattatgcg tgattcccaa ttgcccccag acaggacttg
     2221 acccgtgaaa tccatatcgc tcttcctgct cctcaccttc gcggtgcttg cggccaaggc
     2281 ccaggacatc acgacagccg actccttctt cgcccagatg gccgaccgct acgcccagat
     2341 aaaggattac gagggccatc tcgcgatcac gctcaacgct gacaaggccc ccagcatgac
     2401 gggcagcgtc tccttcaagg cgccgagcct tctgcggatc gacttcatcc agccccccga
     2461 ccaggtcatc gccttcgacg gaaccaccct cacggtctac atcccgagct acagggccgt
     2521 gctctcccag accgccggcg acaagccggg cggagcgggc ggggcctccc tggccagcag
     2581 cgagggcct
//
LOCUS       DASSQV010000245         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_186472, whole genome shotgun sequence.
ACCESSION   DASSQV010000245 DASSQV010000000
VERSION     DASSQV010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186472"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFL04_04175"
     CDS             <1..307
                     /locus_tag="VFL04_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838930.1"
                     /translation="AGLAAVELAALGGPQLDARSLLSLGAAWALSSRLGGHGRVIVPR
                     TAQACWDEAGEGQAWRPDSPELASSLSDVRPDAEIGGRPEELERFLAGLLETRASRP"
     gene            304..1728
                     /locus_tag="VFL04_04180"
     CDS             304..1728
                     /locus_tag="VFL04_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET7838931.1"
                     /translation="MRERYARILELALPLMAGSLVEALYNLTDAFFLGKLGAAEIGAP
                     SIAFSFVVILIVVGSGLSSAGTTLIAQARGRGEAAAANRHLNQTAGFMLMASLALSVL
                     GAPLSPLLLRLVGTPAEVFPYARSYLSIIFLGLPFSYCYFILQASMTAAGDAFSPLKV
                     HLIAVGANVVLAPLLIFGYGPFPRLGVAGAAIATVLAQGLGAFLSLRILIRGRHGLRL
                     RLQEMRPRLEAMALVAKVGLPSSAGMAFEAMGFTVLQGLVNRFGPAAIAAFGVGNRII
                     ALVGIPSAAIGGATTALVGHAIGAGELDRAHSTVRSSLLLCAALLVPPLLLSVAYGPG
                     LVRFFIADPEAMRLGGIMFRIVSPSVLVFGMFVVLTGAFQGAGDTRIIMLLSIVRLWV
                     IRVPLAWALALYGGFGPLSIWYAMFVSNTVVALAGFLHYRAGRWTRALLPGPGDPGWK
                     ASRGPKMAELAVRSVDSNGSDRLN"
     gene            1975..2048
                     /locus_tag="VFL04_04185"
     tRNA            1975..2048
                     /locus_tag="VFL04_04185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2009..2011,aa:Arg,seq:cct)
     gene            complement(2064..>2342)
                     /locus_tag="VFL04_04190"
     CDS             complement(2064..>2342)
                     /locus_tag="VFL04_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7838932.1"
                     /translation="RASGTIAVGLEESAGLIRLSVSDDGVGLGDAGIPRGSTSMGMTL
                     MRMLTEQIGATMRVGSGQGTTIEIEFARDAVKAGEMVGTRGLGIYQEA"
BASE COUNT          301 a          888 c          733 g          420 t
ORIGIN      
        1 ggccggactc gcggccgtcg agctcgccgc cctgggcggg ccccagctcg acgcccggtc
       61 gctcctgagc cttggcgccg cctgggccct ttcgtcccgg ctcgggggcc atggccgcgt
      121 catcgtcccc cgcacggccc aggcctgctg ggacgaggcg ggggagggcc aggcctggag
      181 gcccgacagc cccgagcttg cttcttccct cagcgacgtc agacccgacg ccgagatcgg
      241 gggcaggccc gaggagctcg agcgcttcct cgcaggcctc cttgaaaccc gggcaagccg
      301 cccgtgaggg agcgctacgc caggatcctc gagctcgccc tccccctcat ggcagggagc
      361 ctcgtcgagg ccctttacaa cctgaccgac gccttcttcc tcggcaagct gggggctgcc
      421 gagataggcg ccccctcgat agccttcagc ttcgtcgtca tcctcatcgt cgtcggctcg
      481 ggcctctcca gcgcggggac caccctgatc gcccaggcga ggggcagggg tgaggcagcg
      541 gcggcgaacc gccacctcaa ccagacggcg ggtttcatgc tgatggcctc cctggccctc
      601 tccgtcctgg gcgccccgct cagccccctc ctcctgcgcc tggtcgggac cccggccgag
      661 gtcttcccct acgctcgcag ctacctcagc atcatcttcc tcggcctgcc cttcagctac
      721 tgctatttca tcctccaggc ctcgatgacg gcggccggcg acgccttcag ccccctcaag
      781 gtccacctca tcgcggtcgg ggccaacgtc gtccttgccc ccctcctcat cttcggctac
      841 gggccctttc cccgtctggg cgtggcgggg gcggcgatcg cgacggtgct ggcccagggc
      901 ctcggggcct tcctgtccct ccgcatcctc attcggggcc gccatggcct caggctcagg
      961 ctccaggaga tgcggccccg gctcgaggcg atggccctcg tggccaaggt gggcctgccc
     1021 tcctcggcgg ggatggcctt cgaggccatg ggcttcaccg tcctccaggg cctggtgaac
     1081 cgtttcggcc ccgcggcgat agcggccttc ggggtgggca acaggatcat cgccctcgtg
     1141 ggcatcccct cggcggcgat cggcggggcc acgacggccc tcgtgggcca cgccatcggc
     1201 gcgggggagc tcgaccgggc ccacagcacg gtccgctcct ccctcctcct ctgcgccgcc
     1261 ctcctcgtgc cgcccctcct cctctctgtg gcctacggac ccggcctggt ccgcttcttc
     1321 atcgccgatc ccgaggcgat gcggctgggc gggatcatgt tcaggatcgt gagcccctcg
     1381 gtcctcgtct tcggcatgtt cgtcgtcctg accggggcct ttcagggcgc gggggacacg
     1441 cggatcatca tgctcctctc gatcgtcagg ctttgggtga tcagagtccc cctcgcctgg
     1501 gccctcgccc tctacggcgg tttcggcccc ctctccatct ggtacgccat gttcgtgtcg
     1561 aacaccgtgg tggcgctggc gggtttcctc cattataggg ccggccgctg gaccagggcc
     1621 cttctgccgg gacccggaga cccaggctgg aaggcgagcc gcggcccgaa gatggcggaa
     1681 ttagcggtcc gcagcgttga cagcaacgga agcgataggt taaattaggt tgtggtcgcc
     1741 cagcgaccag gccgcgcaag tggccggagc cgcccccgac ctgccgaaca agagattaaa
     1801 tggcaggtcg cccgtgcggc gctattttta tatagagccc ctcgcccaag gctgagggtt
     1861 cagatacgag ttgggccttg cgggtggctt ttggcgattc cctccgcacc aggcgccttg
     1921 cgcgaaatcg ccggtttgga gagggcatgg aacccatccc ttctccaagc cgctgaccca
     1981 gtagctcagt tggatagagc gactgcctcc taagcagtag gtcgggggtt cgattccctc
     2041 ctgggccacg agaccttcgg ccttcacgcc tcctggtaga tccccagccc ccgagtcccg
     2101 accatctccc ccgccttcac cgcgtcgcgc gcgaactcta tctcgatcgt ggttccctgt
     2161 ccgctcccca cccgcatggt agcgccgatc tgctcggtca gcatccgcat cagggtcatg
     2221 cccatgctcg tggagccccg cgggatcccc gcgtcgccca ggcccacccc gtcgtccgag
     2281 acggagaggc ggatcaggcc cgccgactcc tccagcccca ccgctatggt ccccgaggcc
     2341 ct
//
LOCUS       DASSQV010000246         6530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_931500, whole genome shotgun sequence.
ACCESSION   DASSQV010000246 DASSQV010000000
VERSION     DASSQV010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931500"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFL04_04195"
     CDS             complement(<1..807)
                     /locus_tag="VFL04_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET7838933.1"
                     /translation="MARIIVFVNQKGGVGKTTSAINLGAYLALAGKRTLLVDFDPQAN
                     LTSGVGASAGGRGVYQVVAGQAKMAEVVRATKVPGLSVVPSSIDLSGATVELVDRADR
                     NEYLQRALEPLRSEYDYILIDCPPSLGILTLNGLMAADEVLIPLQCEYFALEGLSLIL
                     QTVSLVQKGMNARLAVSGILFTMYDSRTKLAQDVVQQVTEYFKDRVFRTIVPRNVRLS
                     EAPSHGLPVCLYDPACIGAKSYESLARELIARHNGSSFSLPTRAGAKDAAS"
     gene            941..1165
                     /locus_tag="VFL04_04200"
     CDS             941..1165
                     /locus_tag="VFL04_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET7838934.1"
                     /translation="MLTEKVQRAIQDVRPSLQADGGDIELISVGEDGVVKVKLTGACG
                     SCPMSTITLKQGVENYLKKIIPEVTEVVQA"
     gene            complement(1222..1410)
                     /locus_tag="VFL04_04205"
     CDS             complement(1222..1410)
                     /locus_tag="VFL04_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838935.1"
                     /translation="MATKNYRCKDCGKEVTVEARAAVPECCGEKMQQLPLDPCTHPNV
                     AEAARPGVIDEACDDGVH"
     gene            complement(1497..2621)
                     /locus_tag="VFL04_04210"
     CDS             complement(1497..2621)
                     /locus_tag="VFL04_04210"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET7838936.1"
                     /translation="MPDPVSVLVVDDSALMRNLISKIIESAPGLKVAEKAMNGIFALQ
                     KIPRVDPDVIVLDIEMPEMNGIDFLKERKRLGIEIPVVILSSVAKQGAQVTMEALALG
                     ASDFITKPSGSTSSDIHTVSAQLIRLLIAYGAEYRKTHGKGVPHYEFEAPERLFEPAP
                     LIEERQLSRQKSEAPKPLRSPGPMEIIAIGISTGGPNALREIFSRIDAELKQPVVVVQ
                     HMPPGFTEEFAKSLDRICPLEVKEAADGDIVRQGRILIAPGDRHILVEKRPLGTVVRL
                     SESAPMNGHRPSADVLFESVAKSYGNRAMGVIMTGMGRDGARELGSIFREGGITLGQD
                     ESSSVVYGMPKVAFELGHVTQQVSLSEMADTISRLARQYR"
     gene            complement(2637..3449)
                     /locus_tag="VFL04_04215"
     CDS             complement(2637..3449)
                     /locus_tag="VFL04_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET7838937.1"
                     /translation="MSEFLSDADFDLYRKIIYDESGITFSATNRSILESRLKERLREK
                     KIDSLREYYSLIVKDHEEFKTLLDSVTTNLTRFFRNQAHFDAVEKHVVPELLKIRGSG
                     ERKLRFWSAGCSTGEEPYTIAMLLREILPPAWTFEVIASDISLKCLMVGKEGFYADAR
                     IAGVPDNYLTKYFDKKPGGYQIKDELRKLVRFDYHNLKNDSGLRGLDVIFCSNVLIYF
                     DEAAQKSTIERFWEAMAPKSFLFIGHSESLFGMNTKFEFVKTDWATLYRKFI"
     gene            3637..4107
                     /locus_tag="VFL04_04220"
     CDS             3637..4107
                     /locus_tag="VFL04_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET7838938.1"
                     /translation="MVELRVRGVSVEGPDDSPLVILEDPRNDLRLSVPVGPFEASAII
                     MELEGINPPRPLTHDLLASFFREGGFSLDRVELFGESPDCPRARLAYRRGFKRLGREV
                     RPSDAIALAVRLDAPLIAAAELLKPGRCIDAAPRFLGNRRILSLDDWKSRTIRP"
     gene            4192..5883
                     /locus_tag="VFL04_04225"
     CDS             4192..5883
                     /locus_tag="VFL04_04225"
                     /inference="COORDINATES: protein motif:HMM:NF036553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HET7838939.1"
                     /translation="MSLLGILKEFFVRLLNSDPEESRRRAELRKLYTILAETRPPLYR
                     AKQNLVLPGLAQAILQFYSSLRPLAEIIRVTVASPDVRVSQRYFDFLIECRLPASEQE
                     KRRFFSYEGMSERVAASLQPEQELESISQDFQSFLQSISDLGDKEIDFDLGEVDRFID
                     ICRHDYERLVGLFDPSASLDDPRYKPDFAPVPGEQILPELLDFYYLTEGFFFSPMLKE
                     GVLRLLERRSRNPVDAAKRAKVEKLFSRLDKSLAERLSKDVLGSLIRATKGDPYFLPS
                     TNRERREFMKPYMRRLSTQYEKDRERLLREQHESVVAIDIRGLFGEVDILPLEGYDEE
                     NDAYLRRESPNGFTFIKPLRILKTFIAAAFDPFLKESIKRILVEGYFENKNFQNNLAN
                     ILYQCERSANRIGDFEQQLVGTGRLSITAMRRYIEEMRRGKDISAFLSRLVDAINGRA
                     GEIVEEETGVFAMLGDALGDLLSDYRRSSPDLVTNIRNLGAGRNKEIMGQIQAGRDRI
                     AILVKIMRNFIFVRASIPGALLQGSPATLELRAGAAQGLEAQHPDDLLPEAESAD"
     gene            5948..>6530
                     /locus_tag="VFL04_04230"
     CDS             5948..>6530
                     /locus_tag="VFL04_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HET7838940.1"
                     /translation="MESLGHHCGVAGVYSPDPSNIPETLFYMLFSLQHRGQESCGIAW
                     EEDGSWRSRKDLGMVAPVLGRFVGEDHSSRLGIGHVRYSTAGGAGLQNAQPLVVECNK
                     GRLALGHNGNISNGGSLRSDLTREGSIFQGSADSELLLHLISRSRQTGREAFLKDALA
                     PLEGAYSLVLMHEDALIAIRDPSGFRPLYLGAKD"
BASE COUNT         1218 a         2117 c         1952 g         1243 t
ORIGIN      
        1 gctggcggca tccttggccc cggccctcgt gggcagcgag aagctggagc cgttgtgccg
       61 ggcgatgagc tcgcgggcca gggactcgta gctcttcgcc ccgatgcagg ctggatcgta
      121 gaggcagaca ggcaagccat gggaaggagc ctccgaaagc ctgacattcc tcggcacgat
      181 cgtgcggaac acccgatcct tgaagtactc ggtcacctgc tggaccacgt cctgggccag
      241 ctttgtcctc gagtcgtaca tggtgaagag gatgccggag accgccaacc tggcgttcat
      301 ccccttctgc acaaggctca ccgtctggag gatcagggaa aggccctcga gggcgaagta
      361 ttcgcactgg aggggaatga ggacctcgtc ggccgccatg aggccgttga gggtcaggat
      421 gcccagcgaa ggcgggcagt cgatcaggat gtagtcgtac tcggagcgga gcggttcgag
      481 agcccgctgg aggtattcgt tgcggtcggc cctatcgacg agctcgactg tcgcgccaga
      541 gaggtcgatc gaggatggga ccacggagag acccgggacc ttcgtggccc tcacgacctc
      601 ggccatcttc gcctggcctg cgacgacctg gtagaccccc ctgccccctg ccgatgcccc
      661 gaccccgctt gtcaggttcg cctggggatc gaaatcgacg aggagggtgc gcttccccgc
      721 gagggcaagg tatgcgccga gattgatggc gctcgtggtc ttgcccactc cgcctttctg
      781 attcacgaaa acgatgattc gcgccattgc gcctctccgg gcttgatatg ccgagtgtaa
      841 cacaaggtgc cagcccatcc aaggcgggct ctggctcatg gatgctcaac ttgacgcgcc
      901 aaacctgggc tggctacatt ggtggcaagg agaacagccc atgctaaccg aaaaggtcca
      961 gcgcgccata caggacgtca ggccatcgct ccaggccgat ggcggagaca tcgaactcat
     1021 ttcggtggga gaggatggcg tcgtcaaggt caagctcacc ggtgcctgcg gatcctgccc
     1081 catgtcgacg atcaccctca agcagggcgt cgagaactat ctcaagaaga tcatccccga
     1141 agtgacggaa gtcgtccagg cataataaat cggccgcccc atggcggccg attccatttc
     1201 tgggcagctt cctgcgtgcg actagtgcac gccgtcgtcg caggcttcgt cgatgacacc
     1261 cggcctcgcg gcctcggcga cgttgggatg agtgcaggga tccaagggca gctgctgcat
     1321 cttctcgccg cagcattccg gcaccgccgc tcgggcctcg acggtcacct ccttcccgca
     1381 atccttgcac ctgtagtttt ttgtagccat tcggacctcc tggagcggat cgctccgtcg
     1441 atcgtgccta tgtgctcaag atactgcgcc ccgtgagagc gggaaaggcg ctcgactcat
     1501 cgatactgcc tcgcaagcct gctgatcgtg tccgccatct cggacaggga cacctgctgg
     1561 gtcacgtggc ccaactcgaa ggcgaccttg ggcatgccgt acaccacgct cgatgattcg
     1621 tcctgcccca aggtgatgcc cccctcccgg aatatggacc cgagttccct cgccccatcc
     1681 cggcccattc ccgtcatgat gacccccatc gcccggttcc catagctctt ggcgacgctc
     1741 tcgaagagca catcggcgct cggcctgtgg ccgttcatcg gcgccgactc ggaaagcctt
     1801 accactgtgc cgagcgggcg cttctcgacc aggatgtggc ggtcccccgg cgctatcagg
     1861 atgcgaccct gcctgacgat gtcgccgtcg gccgcctcct tcacctcgag cgggcagatc
     1921 ctgtccagac tcttggcgaa ctcctcggtg aagcccgggg gcatgtgctg gacgacgacc
     1981 acgggctgct tgagttcggc atcgatcctg gaaaagatct cccgcagggc gttcggtcca
     2041 cccgtggaga tcccgatggc gatgatctcc atcggccccg gcgaccgaag gggcttggga
     2101 gcctcgctct tttgcctgga gagctgtcgc tcctcaatca ggggggcggg ctcgaacaga
     2161 cgctccggag cctcgaattc gtagtggggc acgcccttgc cgtgggtctt gcggtattcg
     2221 gccccataag cgatcaggag acggatgagc tgggccgaga cggtatggat atcggaggat
     2281 gttgatcctg agggcttggt gatgaagtcg gaggctccga gcgcgagcgc ctccatcgtg
     2341 acctgggcgc cctgcttggc caccgatgac aggatgacga cggggatctc gatcccgaga
     2401 cgcttccttt ccttgagaaa atcgatcccg ttcatctccg gcatctcaat gtccaggact
     2461 atcacgtcgg ggtcgacgcg tgggatcttc tggagcgcga agatgccatt catcgccttc
     2521 tcggcgacct tcaggcccgg ggcggattcg atgattttgg aaatcaggtt ccgcatgagg
     2581 gcggagtcgt cgactacgag gacggagact ggatctggca cgtctgtcgc tcccctttag
     2641 atgaatttgc gataaagggt ggcccagtcg gtcttcacga actcgaactt cgtattcatg
     2701 ccgaacaggg actcggagtg gccgatgaaa aggaaggact tcggggccat ggcttcccag
     2761 aagcgctcga tcgtggactt ctgggcggcc tcatcgaagt agatcagtac gttactgcag
     2821 aagatgacgt ccagaccgcg aaggcccgaa tcgttcttca ggttgtggta gtcgaagcgc
     2881 accagcttcc ggagctcatc cttgatctgg taaccacccg gtttcttgtc gaagtatttc
     2941 gtcaggtagt tgtcggggac cccggcgatc cgcgcgtcgg cgtagaagcc ctccttcccg
     3001 accatgaggc atttgaggga gatgtcgctg gcaatcacct cgaaggtcca cgcagggggc
     3061 agaatctcgc ggaggagcat cgctatggtg tagggttcct ccccggtcga gcagcccgcg
     3121 ctccagaacc taagcttccg ctccccgctg cccctgatct tgaggagctc gggcaccaca
     3181 tgcttctcga cggcatcgaa gtgggcctgg ttccggaaga acctggtcag gttggtcgtc
     3241 accgaatcta ggagggtctt gaactcctca tgatccttga cgatcaggga gtagtattcg
     3301 cggaggctgt cgatcttctt ctctcgcaac ctctccttca gccggctctc gaggatggat
     3361 cggttggtgg ccgagaaggt tatgccgctc tcatcgtaga tgatcttgcg atacagatca
     3421 aagtcggcat cggacaggaa ctcagacatg cctctcgatt ccgcctttcc cagcgatcac
     3481 cgagcctcgc atggtacaaa aaagcatagg cggggcccgg aggggtgtca atagcgggcc
     3541 tggggcgagg ctggaccggc catggcggat tcctttcccc gccgatgcgg aattcccaat
     3601 gacatcggga tagactggac ccttatggat gtaggcatgg tcgagctccg cgtccgggga
     3661 gtatcggtgg aagggcctga cgattccccc cttgtcattc tcgaagaccc ccgcaacgat
     3721 ctccgcctct cggtccccgt cgggcctttc gaggcgagcg ccatcatcat ggagctggag
     3781 ggcatcaatc ctccgcggcc gctcacccac gaccttctcg cgtccttttt cagagaaggc
     3841 ggattctccc tcgatagggt cgaactcttc ggcgagtcgc ccgattgccc gcgtgctcgc
     3901 ctggcctaca ggcggggctt caagcggctg ggacgcgaag tgaggccctc ggacgccata
     3961 gccttggcag taaggctgga cgctcccctg atcgccgccg ccgagctcct gaaaccggga
     4021 cgctgcatcg atgcggcccc gaggttcctc ggaaacagga ggatcctctc gctcgacgac
     4081 tggaaatcaa ggaccattcg gccctagccc gcatcagtcg gggccggacc cctcccggcc
     4141 caagtccaaa tcccttgctg tcgcgccctc cgctcaccta caatttaggc catgagtctc
     4201 ctcgggatac taaaagaatt cttcgtacgc ctgctcaact ccgatccgga ggagagccgg
     4261 cgcagggccg aactccgaaa gctctacacc atcctcgcgg agacgaggcc ccccctctat
     4321 cgggcaaagc agaacctggt cctccccggc ttggcccagg ccatcctcca gttctactcc
     4381 tccctccgcc ctctggccga gatcatcagg gtgacggtcg cgtctcccga tgtcagggtg
     4441 tcccaaaggt atttcgactt cctgatcgaa tgccggcttc ccgcgtcgga gcaggagaag
     4501 cggcgcttct tctcctacga gggcatgtct gaacgggtcg ccgcctccct ccagccggag
     4561 caggaactgg agtcgatctc gcaggatttc cagagcttcc ttcagtccat ctctgacctt
     4621 ggcgacaagg agatcgactt cgaccttggc gaggtggacc gtttcatcga tatctgcagg
     4681 catgactacg aacgccttgt cggcctcttc gatcccagcg caagcctcga tgatccccga
     4741 tacaaaccag acttcgcccc cgtacccggg gagcagatcc tgcccgagct cctggatttc
     4801 tactacctta ccgaaggctt cttcttctcc cccatgctca aggagggcgt cctgcgtctc
     4861 ctcgagcggc ggtcccggaa tcccgtcgac gcagctaaac gcgccaaagt cgagaagctc
     4921 ttctcccgcc tcgacaagtc cctcgccgaa cgcctgagca aggacgtcct cggctccctc
     4981 atccgcgcga caaaggggga tccatatttc ctgccctcga caaaccgcga gcgccgcgag
     5041 ttcatgaaac cctacatgcg ccgcctttcc acccagtacg agaaggacag ggagcggctc
     5101 ctcagggaac agcacgagag tgtcgtagcg atcgacatcc gtgggctttt cggggaggtg
     5161 gacatcctcc ccctcgaggg ctatgacgag gagaacgacg cctacctcag gcgagagagc
     5221 ccgaacgggt tcaccttcat caagccgctg cggatactca agaccttcat cgccgccgcc
     5281 ttcgatccct tcctcaagga gagcatcaag cggatccttg tcgagggcta tttcgagaac
     5341 aagaacttcc agaacaacct cgcgaacatt ctctaccagt gcgagcgctc cgcgaaccgc
     5401 atcggtgatt tcgaacagca gctggtcgga actgggaggc tgtccataac cgccatgcgc
     5461 cgatacatcg aggagatgag gagggggaag gacatctctg ccttcctgtc ccggctcgtt
     5521 gacgccatca acggccgggc gggggagatc gtcgaggagg agaccggagt gttcgcgatg
     5581 ctcggcgatg ccctgggcga cctcctctcc gactaccgcc gctcctctcc cgacctcgtc
     5641 acgaacatca gaaacctcgg cgccgggcgg aacaaggaga tcatggggca gatccaggcg
     5701 ggcagggatc gcatcgcgat cctggtcaag atcatgcgca atttcatctt cgtcagggcc
     5761 agcatccccg gtgccctcct ccagggttcg cccgcgacgc tggagctgcg cgcaggcgcc
     5821 gcacagggcc ttgaggcgca gcatcccgat gaccttctcc cggaggctga gagcgcggac
     5881 tagagccccg aaccgccggc gcccccgcga gcttgacctt cctcgaaccg acgtgctaga
     5941 accggacatg gaaagcctag gccaccactg cggcgtcgcc ggcgtctact cccccgatcc
     6001 ctccaacatc cccgagaccc tcttctacat gctcttctcc ctgcagcacc gcggccagga
     6061 atcctgcggg atcgcttggg aggaggacgg cagctggaga tcccggaagg acctcgggat
     6121 ggtcgctccc gtccttggcc gttttgtcgg ggaggaccac tcgtcccgcc tcgggatcgg
     6181 ccatgtccgg tattcgaccg cgggaggagc ggggctccag aacgcccagc ccctcgtcgt
     6241 ggaatgcaac aagggaaggc tcgccctcgg ccacaacggc aacatctcga acgggggaag
     6301 cctgaggtcg gacctcaccc gggaaggctc gatcttccag ggcagcgccg attccgaact
     6361 cctcctccac ctcatctcga ggtcgagaca aacgggccgc gaggccttcc taaaggacgc
     6421 cctggctccg ctcgagggag cctatagctt ggtcctcatg cacgaggatg ccctcatcgc
     6481 catccgcgac ccctcgggct tcagacccct ctacctcggc gcgaaggacg
//
LOCUS       DASSQV010000247         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2050811, whole genome shotgun sequence.
ACCESSION   DASSQV010000247 DASSQV010000000
VERSION     DASSQV010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050811"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VFL04_04235"
     CDS             complement(<1..1244)
                     /locus_tag="VFL04_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HET7838941.1"
                     /translation="MKTIAVIEIGSTGIRLVVAEIDGEGGFKVLDRAGKPSRVGRDVF
                     TSGYVSREALRESTAMLSSYRELLRGYGIAPEDSRVIATSALREASNRDTFVDRVSLQ
                     TGFRVNIVEDIEENHLMYLGVQHALQDERRRLSKSNAMILEVGGGSTEIMLLRRGKMT
                     GAHSLRIGTVRVDEQVRGAGVSPTYLRQFLEDNVRTACDNLDSELPLDSVKTFIVIGS
                     DARLAATRLASESFEGYAVVDRARFIDFANEISALSAEDCVARLKLPWSEAEGLGAGL
                     AIESLFLERTDAETVVVPNVSIREGVLLSMSRGPDVDLEAEMRRQIIASADSLGRKFR
                     YDEPHARHVADLCLCLFDGLMREHGLGARERLLLETAALLHDIGTYIRASGHHKHGEY
                     IVANSEIFGLNRSDLTIVSNVVR"
     gene            complement(1241..2212)
                     /locus_tag="VFL04_04240"
     CDS             complement(1241..2212)
                     /locus_tag="VFL04_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7838942.1"
                     /translation="MAKDARVYIGVDLGATKIIAALFDSKLKYLSSEKIKTPREGSAQ
                     AVVKALSECVLRTLADAGEGAVLAAVGVAVPGPLDRAKGIVRYTPNMGFKDYHLGEEL
                     AGRFGAPAVLENDVQAGVLGELRAGALKGMRHAVGVFIGTGIGGGIVMNGELYRGATG
                     SAGEVGHMILQDGGALCGCGNYGCLEALASRTAMAKDALALAASGKAPALIDIAGTDI
                     RKYRSSAFEEAIANGDKKVKVVLDRAAYWVGVGLANLVHVLNPEAIVLGGGMATRFGE
                     GFRKKAEASMEAHLMPGLSGTSRLLLSELGDLAVPTGAAFAASEAGA"
     gene            2344..>3548
                     /locus_tag="VFL04_04245"
     CDS             2344..>3548
                     /locus_tag="VFL04_04245"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7838943.1"
                     /translation="MTRRTYRATRLLVTLLAALGLAMGISACSGRLGWGVVLWTAPEG
                     PVKAGSVVPVYIKSNIQKLYVVGLPDGSKKIELPLWQVELFPTKAKAEARVKRFGDYL
                     GIYMVAARDGLPIREGPFGGARRVYRLREGQSVKVLDKVQGEAVTTGGEALPGDWYQV
                     LSDDGTKGFVFSYAMRQYDESKEGPPAGLVTKAPSGRVDLVFSRQWRPEYFQEMLDDQ
                     RVDLDLFSMRFGIFADAVRKQIRIELPAASQIFNYDSISEDGGAYVFEGTPLRISLEG
                     DRRLVATWTDQASAPPPTAGGQDQAGQDQGSSSPPAAQASPSSYGFEGSAAFTVPSLE
                     PREAIRLEELRQQRLLESFLDKFGSEWASDAGGRLLLSKSRRITWTERTALPTGVLPE
                     TPSDTGEIA"
BASE COUNT          534 a         1239 c         1214 g          561 t
ORIGIN      
        1 cgcacaacgt tcgagacgat ggtcaaatcg ctgcggttga ggccgaagat ctccgagttc
       61 gccacgatgt actcgccgtg cttgtggtgg cccgaggccc tgatataagt cccgatgtcg
      121 tggaggaggg cggcggtctc gaggaggagg cgctcgcggg cccccaggcc gtgctcccgc
      181 atgaggccgt cgaagaggca gaggcagagg tcggccacgt ggcgggcgtg gggctcgtcg
      241 tagcggaact tccgcccgag ggagtccgcg ctcgcgatga tctggcggcg catctcggcc
      301 tcgaggtcga cgtcggggcc ccgcgacatg gagaggagga cgccctcgcg tatggacacg
      361 ttgggcacca ccaccgtctc ggcgtcggtc cgctcgagga agaggctctc gatggcgagg
      421 ccggcgccga ggccctcggc ctcggaccag gggagcttga gcctcgcgac gcagtcctcg
      481 gccgagaggg ccgagatctc gttcgcgaag tcgatgaatc tggccctgtc caccaccgcg
      541 tagccttcga aggactcgga ggcgagcctc gtggccgcga gcctggcgtc gctcccgatg
      601 acgatgaagg tcttcaccga gtccaggggc agctccgagt cgaggttgtc gcaggcggtc
      661 ctgacgttgt cctccaggaa ctgcctgagg taggttggcg agaccccggc tccgcgcacc
      721 tgctcgtcca ccctgaccgt gccgatgcgg agggaatggg cccccgtcat cttgccccgc
      781 ctgaggagca tgatctccgt cgagccgccg ccgacctcga ggatcatggc gttggacttg
      841 gagagccgcc gcctctcgtc ctggagggcg tgctggaccc cgaggtacat gaggtggttc
      901 tcctcgatgt cctcgacgat gttcaccctg aagcctgtct ggagggagac cctgtccacg
      961 aaggtgtcgc ggttggaggc ctccctgagg gcgctcgtgg cgatgaccct ggagtcctcg
     1021 ggggcgatgc cgtagccccg gaggagctcg cggtagctcg agagcatcgc cgtgctctcg
     1081 cggagggcct cccggctcac atagcccgag gtgaagacgt cgcggccgac cctgctcggc
     1141 ttcccggcgc ggtcgaggac cttgaagccg ccctcgccgt ctatctccgc gacgacgagg
     1201 cggatgcccg tcgagccgat ctctatgacc gcgatcgtct tcacgcgccg gcctcggagg
     1261 ccgcgaacgc agcccccgtc gggacggcga ggtcgccgag ctcggagagg aggagcctgg
     1321 acgtgcccga gagccccggc atgaggtggg cctccatcga ggcctcggcc ttcttgcgga
     1381 agccctcgcc gaagcgggtc gccatgcccc ctccgaggac gatggcctcg gggttgagga
     1441 cgtgcaccag gttcgcgagg ccgaccccga cccagtaggc ggccctgtcc aggaccacct
     1501 tgaccttctt gtccccgttg gcgatggcct cctcgaaggc cgagctccgg tatttgcgga
     1561 tgtcggtgcc ggctatgtcg atgagggcag gggccttgcc cgaggccgcg agggccaggg
     1621 cgtccttggc catggccgtg cgcgaggcga gggcctcgag gcagccgtag ttgccgcagc
     1681 cgcagagcgc gcccccgtcc tggaggatca tgtggcccac ctctccggcc gagcccgtgg
     1741 cgccccggta gagctcgccg ttcatgacga tgcccccgcc gatccccgtg ccgatgaaga
     1801 cgccgaccgc gtggcgcatg cccttgaggg ccccagccct gagctcgccg aggaccccgg
     1861 cctggacgtc gttctcgagc acggccggag ccccgaaacg gcccgccagc tcctcgccga
     1921 ggtggtagtc cttgaagccc atgttgggcg tgtagcggac gatgcccttg gcccggtcga
     1981 gggggccggg gacggcgacg ccgaccgcag ccaggaccgc cccctccccc gcgtcggcga
     2041 gggtacggag cacgcattcg gagagggcct tgaccacggc ctgggcgctg ccctccctcg
     2101 gggtcttgat cttctcggag gagaggtact tgagcttgga gtcgaaaagg gcggcgatga
     2161 tcttggtggc cccgagatcg acaccgatgt agacgcgagc atccttggcc atatctcccc
     2221 ctgcctccgc ccccggcgat gccccgggcg cggctccatc cactatagca tactcgttcc
     2281 ccgccatcgg atccggcccc ccgatattgc ggagctcggc cccgctcatg tagactgctg
     2341 gcaatgacgc gccggaccta ccgtgccacg aggctccttg tcaccctcct cgccgccctc
     2401 ggactcgcga tgggcatctc ggcctgctcc ggccgcctcg gctggggcgt cgtgctctgg
     2461 acggcccccg aggggccggt caaggcgggc agcgtcgtgc cggtctacat caaatccaac
     2521 atccagaagc tctacgtggt cggcctcccc gacggatcga agaagatcga gctccccctc
     2581 tggcaggtcg agctcttccc cacgaaggcc aaggccgagg ccagggtcaa gcgtttcggc
     2641 gactacctcg gcatctacat ggtcgcggcc cgggatggcc tgcccatcag ggaaggcccc
     2701 ttcggcgggg cgaggcgggt gtaccggctc cgcgaggggc agtcggtcaa ggtcctggac
     2761 aaggtccagg gggaggccgt cacgacgggg ggcgaggccc tgcccgggga ctggtaccag
     2821 gtcctctccg acgacgggac caagggcttc gtcttcagct acgccatgag gcagtacgac
     2881 gagtccaagg agggtccgcc ggcggggctc gtgaccaagg cgccctcggg ccgcgtcgac
     2941 ctcgtcttct cgcgccagtg gaggcccgag tacttccagg agatgctcga cgaccagagg
     3001 gtcgacctcg acctcttcag catgcgtttc ggcatcttcg ccgacgctgt gaggaagcag
     3061 atccgcatcg agctcccggc cgcgtcccag atcttcaact atgactcgat ctccgaggac
     3121 gggggggcct acgttttcga gggcaccccg cttaggatca gcctcgaggg cgaccgccgc
     3181 cttgtggcca cctggaccga ccaggcctcg gccccccctc ccaccgcggg cggccaggat
     3241 caggccggcc aggaccaggg ctcaagcagc cctcccgcgg cccaggcctc cccctcatcc
     3301 tacggattcg agggtagcgc cgccttcacc gtgccctccc tcgagccccg cgaggcgata
     3361 cggctcgagg agctcagaca gcagaggctc ctcgagtcct ttctcgacaa gttcggatcc
     3421 gagtgggcct cggacgccgg cgggcgcctc ctcctgtcca agagccggcg catcacctgg
     3481 accgaaagga cggccctccc caccggcgtc ctgcccgaga ccccgagcga caccggcgag
     3541 atagcctt
//
LOCUS       DASSQV010000248         4884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1676874, whole genome shotgun sequence.
ACCESSION   DASSQV010000248 DASSQV010000000
VERSION     DASSQV010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676874"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /gene="asd"
                     /locus_tag="VFL04_04250"
     CDS             complement(<1..958)
                     /gene="asd"
                     /locus_tag="VFL04_04250"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET7838944.1"
                     /translation="MSRIPVTVLGATGVVGQRFVRRLARHPSFEISHLTASERNAGTR
                     YAEACDWRLEGEPYAGLAELVLQVCDPERAYAPIVFSALDAEVALRVEPAFAKAGALV
                     FSNASAFRMEEDVPLLVPELNAEHLGLLELQRRRRAWTGGIVCNPNCTTAVLLGALGP
                     LHRAFGAEAALAVSMQALSGAGYPGVSSIDATANVIPFIRGEEDKVETEARKILGRLE
                     GGRISEAPIALSASCNRVPVVEGHTIAASLRLGGSPSPAEIVAALEAFVPDTEGLGLH
                     SSPARFLQVRREPDRPQPRRDVEEAGGMMIQVGRVRSCPILGA"
     gene            complement(989..1126)
                     /locus_tag="VFL04_04255"
     CDS             complement(989..1126)
                     /locus_tag="VFL04_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838945.1"
                     /translation="MRKLMSLNAAKALAKAKRRLADIEAFVRRAMAISLTRSRPGRAN
                     Q"
     regulatory      complement(1201..1386)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(1470..2042)
                     /locus_tag="VFL04_04260"
     CDS             complement(1470..2042)
                     /locus_tag="VFL04_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET7838946.1"
                     /translation="MSLPEKGSPALPPDWRGRAYLALDFETTGLDPKLDRVVEIGAIR
                     FRVGEDGKALEEGRISACVDPCMPISPAARAVNGISDEELVGAPLFSDFALPLLELGA
                     GATIVAHNAPFDLAFLREELARSGLPEAPNEVLDTRHLAKAAFPARRGYRLTHLASDL
                     ALDTGRAHRALDDARTCMALFMVCAGMLDS"
     gene            complement(2039..3808)
                     /locus_tag="VFL04_04265"
     CDS             complement(2039..3808)
                     /locus_tag="VFL04_04265"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET7838947.1"
                     /translation="MDHDLIKTKATDYIAREIDHDFRTEVVDLMAKGDWKELEDRFYR
                     DLEFGTGGLRGVIGGGYNRMNSFVVTRATQGLCDYLKKCFPGKALSACVAFDSRHHSD
                     TFAEATALVFAANGIKAYLFSSLRPTPELSYAIRKLGADTGVVVTASHNPPKYNGYKA
                     YWNDGAQIIAPHDEGVIAMVNAAQNAKTMHRDEAVAKGLLVSIDKEVDEAYVAMVKSK
                     LLRPELIKRMAASVKIVYTPLHGTGAYLFERIMGELGLKVTTVPEQREPNGDFPTVSF
                     PNPEEPAALKLALELGRKVGADAVMATDPDADRLGIAVPAAGGFTLITGNQLGALHLD
                     YILLTRKELGLLPRKPAVIKSIVTTDLQTRIASAYDARTFECLTGFKWIADLMRRFET
                     DGKGYEFVYGTEESYGHLIENDVRDKDGISAAALTAEMTLYWRSKGKSLLERLDELFR
                     EHGLFEEKGLNKYFEGAGGMDIMRGIMDGYRKSQPSTLGGIRVLSIRDVKTGLEWEAG
                     QAASARKIELPTSDVIQWRLEDGTLVTVRPSGTEPKIKYYILLRTEIGRGGDPAKAMA
                     EARASSAAKASAIEADIRKVIGA"
     gene            complement(3862..4509)
                     /locus_tag="VFL04_04270"
     CDS             complement(3862..4509)
                     /locus_tag="VFL04_04270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7838948.1"
                     /translation="MHKAVIFDLDGTLADTLEDLARAVNGALGRRGFPLHPLDAYRRM
                     VGDGFRLLVTRALPEDQRLPDRIEELRAEASADYEEHCLVSTRPYEGVPELLAALHAR
                     GIPLAILSNKPHELCLRVVAGLFPGAGFALVRGEAPGFPRKPDPASALDIARRLDLPP
                     SEFAYLGDSDVDMSTAKAAGMTALGAGWGFRGAPELAAAGAAAILGSPMELLDFL"
     gene            4701..>4884
                     /locus_tag="VFL04_04275"
     CDS             4701..>4884
                     /locus_tag="VFL04_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838949.1"
                     /translation="MILFRIAFRNLLRRSRKNIAIGALIAVSVAVFFTGNAVLESSVS
                     GIQRTFSEQFTADLSVS"
BASE COUNT          686 a         1581 c         1793 g          824 t
ORIGIN      
        1 tggccccgag gatggggcag gatcggaccc ttcccacctg gatcatcatg ccccctgcct
       61 cctcgacgtc gcggcggggc tgggggcggt cgggctcgcg cctgacctgg aggaagcgtg
      121 caggcgagct gtggagcccg aggccctcgg tgtcggggac gaaggcctcg agggcggcga
      181 cgatttcggc gggggagggg gagcccccga ggcggaggct cgccgctatc gtgtggccct
      241 cgaccacggg cacgcggttg cacgatgccg aaagggcgat gggggcctcc gagatcctcc
      301 cgccctcgag gcggccgagg atcttcctgg cctcggtctc gaccttgtcc tcctcgcccc
      361 tgatgaaggg tatgacgttc gcggtggcgt cgatcgagga gacccccgga tagccggccc
      421 cgctcagggc ctgcatgctc acggcgaggg cggcctcggc gccgaaggcc cggtggaggg
      481 gcccgagggc gccgaggagg acggccgttg tgcagttcgg gttgcagacg atcccgcccg
      541 tccaggccct ccttctgcgc tggagctcga ggaggccgag gtgctcggcg ttgagctcgg
      601 gcacgaggag ggggacgtcc tcctccatgc ggaaggccga ggcgttgctg aagacaagcg
      661 cgcctgcctt ggcgaaggcc ggctcgaccc tgagggcgac ctccgcgtcg agggcgctga
      721 agactatcgg cgcgtatgcc cgctcggggt cgcagacctg gaggacgagc tcggccagcc
      781 cggcgtaggg ctcgccctcg agcctccagt cgcaggcctc ggcgtagcgc gtgcccgcgt
      841 tcctctcgct cgcggtgagg tggctgatct cgaatgaggg atggcgcgcg aggcgcctca
      901 cgaaacgttg gccgacgacg cctgtggcgc cgagcacggt cacgggaatt ctggacatgg
      961 gatcctctcg cctctgtggg tgattcggct attggttggc ccggccgggt cgggatcgcg
     1021 taaggctgat cgccatcgcc cttcgtacga aggcttcgat gtcggccagc ctgcgcttcg
     1081 ccttggcgag ggcctttgcc gcgttgaggc tcatgagttt ccgcatcgga gggacctcct
     1141 gtccgatgga gatcccatga aaccatgccc gtgaccctgt ggggtcagcc gtggtgggcc
     1201 ggttccgatg gcactccgcg gggaggggct ccccgcgaca gccgtggggt cttgacccct
     1261 tgcgtccaag gaggggcggc gtggcggccc ccctcccttc ggcgatcctc ccccttcgtc
     1321 cggcctcaac gggcgccatg ccctcccgcc gtcctactca tggggatcgc tcctccagtc
     1381 ggtactatgg aaagtatcat ggcccccgcc cgggcggcaa gtggccggcg ctggcgagtg
     1441 gccgcggtcc cggcccgtgc cggctagggc taggagtcga gcatccccgc gcagaccatg
     1501 aagagggcca tgcaggtcct cgcgtcgtcg agggcccggt gggcgcggcc ggtgtcgagg
     1561 gcgaggtcgg aggcgaggtg ggtcaggcgg tagccccttc gggccgggaa ggccgccttg
     1621 gccaggtgcc tcgtgtcgag gacctcgttc ggggcctcgg gcaggcccga gcgcgcgagc
     1681 tcctcgcgga ggaaggcgag gtcgaagggg gcgttgtgcg ccactatcgt cgcccccgcg
     1741 ccgagctcga ggagggggag ggcgaagtcg gagaacagcg gcgccccgac gagctcctcg
     1801 tcgctgatcc cgttcacggc ccgggccgcc ggcgagatcg gcatgcaggg gtccacgcag
     1861 gccgagatcc gcccctcctc cagggccttc ccgtcctcgc ccaccctgaa gcggatggcc
     1921 ccgatctcca ccacgcggtc gagctttggg tcgaggccgg tcgtctcgaa gtccagcgcg
     1981 aggtaggccc gccccctcca gtccgggggg agggccgggc tccccttttc ggggaggctc
     2041 acgcgccgat gaccttgcgg atgtcggcct cgatggccga ggccttggcg gccgagctgg
     2101 cgcgcgcctc ggccatggcc ttcgcggggt ccccgcccct gccgatctcg gtgcggagga
     2161 ggatgtagta cttgatcttg ggctcggtgc ccgagggccg cacggtcacg agggtcccgt
     2221 cctcgagcct ccactggatc acgtcgctcg tcgggagctc gatcttccgc gccgaggcgg
     2281 cctgacccgc ttcccactcg aggccggtct tcacgtccct gatgctgagc accctgatcc
     2341 cgccgagggt ggatggctgg ctcttccggt agccgtccat gatgccccgc atgatgtcca
     2401 tgccgcccgc cccctcgaag tacttgttga ggcccttctc ctcgaagagg ccgtgctcgc
     2461 ggaagagctc gtcaaggcgc tcgaggaggc tcttgccctt ggacctccag tagagggtca
     2521 tctcggcggt gagggcggcc gccgagatcc cgtccttgtc gcggacatcg ttctcgatga
     2581 ggtggccgta gctctcctcg gtcccgtaga cgaactcgta gcccttgccg tcggtctcga
     2641 agcggcgcat gaggtcggcg atccacttga agccggtcag gcactcgaag gtcctcgcgt
     2701 cgtaggccga ggcgatgcgg gtctggaggt cggtggtcac gatgctcttg atgacggccg
     2761 gcttccttgg caggaggccg agctccttcc gggtgaggag gatatagtcg aggtggaggg
     2821 caccgagctg gttgcctgtg atgagggtga agccgccggc ggcggggacg gcgatcccca
     2881 gcctgtcggc gtcggggtcg gtggccatga cggcgtcggc cccgaccttg cggccaagct
     2941 cgagggcgag cttgagggcg gccggctcct cggggttcgg gaaggagacc gtcgggaaat
     3001 ccccgttggg ctcgcgctgc tcgggcaccg tggtcacctt gaggccgagc tcgcccatga
     3061 tccgctcgaa gagataggcc cccgtcccgt gcaggggggt gtacacgatc ttcaccgagg
     3121 cggccatcct cttgatgagc tcgggccgca ggagcttcga cttgaccatc gcgacatagg
     3181 cctcgtcgac ctccttgtcg atggagacca ggaggccctt cgcgacggcc tcgtcgcggt
     3241 gcatggtctt ggcgttctgc gccgcgttga ccatcgcgat gacgccctcg tcgtgggggg
     3301 cgatgatctg ggccccgtcg ttccagtagg ccttgtagcc gttgtacttg ggcgggttgt
     3361 ggctggccgt tacgacgacc ccggtgtccg cgccgagctt ccgtatggcg taggagagct
     3421 cgggcgtggg cctgagcgag ctgaagaggt aggccttgat cccgttggcg gcgaagacca
     3481 gggccgtggc ctcggcgaag gtgtcggaat ggtggcggga gtcgaaggcg acgcaggcgg
     3541 agagggcctt gccggggaag catttcttga ggtagtcgca gaggccttgg gtggccctcg
     3601 tgacgacgaa gctgttcatg cggttgtagc cgccgccgat caccccgcgg aggccccccg
     3661 tgccgaactc gaggtcgcgg tagaatcggt cctcgagctc cttccagtcc cccttggcca
     3721 tcaggtccac gacctcggtg cggaagtcat ggtcgatctc ccgggcgatg tagtcggtgg
     3781 ctttggtctt gatgaggtcg tggtccatgg cggtactcct tatcgaaaat tactgcggag
     3841 gggctccgct cgtatcggct ctcagaggaa atccagcagc tccatggggg agcccaggat
     3901 ggcggccgcc cccgccgcgg cgagctcggg ggcgccgcgg aagccccagc ccgccccaag
     3961 ggccgtcatc cccgcggcct tcgccgtgct catgtcgacg tcggagtcgc cgaggtaggc
     4021 gaactccgaa gggggaaggt cgagacgccg ggctatgtcg agggcagagg ccgggtcggg
     4081 cttgcgcggg aatccggggg cctcccccct cacgagggcg aaacccgcgc ccggaaagag
     4141 cccggcgacg accctgaggc agagctcatg gggcttgttc gagaggatcg cgagggggat
     4201 cccgcgggca tggagggcgg ccaagagctc gggcacgccc tcgtagggcc gggtggagac
     4261 gaggcagtgc tcctcatagt cggccgaggc ctcggcgcgg agctcctcga tgcggtcggg
     4321 gagcctctgg tcttcgggga gggcgcgggt gacaaggagc cgaaagccgt cgccgaccat
     4381 cctccggtag gcgtcgaggg gatggagggg aaagccgcgc cggccaaggg cgccgttcac
     4441 ggcccgggcg aggtcctcga gggtatcggc aagggtgccg tcgagatcga atatcacagc
     4501 tttgtgcatt tccgcgccct tcgccggccg caggggcggc cacagggacc agtatcgtcg
     4561 agccccaagg ccatcgtcaa ttgcccccat ggcgccgaag ccggggatcg gcggccaaag
     4621 cttgtatcgg caagggggcc cgtgctaatc tctgggctgt ccgttcaccg agtgaacact
     4681 aacccagggc ggttatccgg atgatcctgt ttcgcatcgc cttccgcaac ctcctccgcc
     4741 gaagccgcaa gaacattgcc atcggggcac tcatcgcggt gagcgtagcc gtgttcttca
     4801 cgggcaacgc cgtcctcgag agctcggtga gcgggatcca gcgcaccttc tccgaacagt
     4861 tcacggccga cctctcggtc agcg
//
LOCUS       DASSQV010000249         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_682825, whole genome shotgun sequence.
ACCESSION   DASSQV010000249 DASSQV010000000
VERSION     DASSQV010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682825"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1918
                     /gene="recG"
                     /locus_tag="VFL04_04280"
     CDS             <1..1918
                     /gene="recG"
                     /locus_tag="VFL04_04280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET7838950.1"
                     /translation="SAFAKEPRVHTVATVVEHEWFGFGRMRTLKIRVEDGSGRAVLVC
                     FNRPFLENSLPVGARVLVTGKFGFRYGEIQSSAFEAERLDGEGGAKEGILPVYALTDG
                     LAQGLVRRLAKRAIVEYGSKVEDELPPPLMVRRGLLPKAEALAKVHFPPSLAELERAR
                     SSLIFEELFYFQLAVARRAMRRREAAVPRAAVRGLLKARLVERLPFALTADQLKASDE
                     IAADMGSPRPMARLLQGDVGSGKTLVAFLACLDAIEAGGQAALMAPTELLARQHASTA
                     ARFLEPLGIRLAFLSGNVEDAARPPLLAALKAGEVDLGLGTQALFSEEVEYRNLRLAV
                     VDEQHRFGVLQRLALGRKGPLPDLLMMTATPIPRTLALTVFGDLEVSAIRTMPPGRKS
                     VITHLAKAGNEKRVYEFVRGLLAQGRQAYFVYPLIGGSERPSSKELKNAEAMAEHLRT
                     AVFPEYSLGLIHSRLKEEEKRSTMEAFARGQTRILVATSVVEVGVDVPNATAMVIEHA
                     ERFGLAALHQLRGRVGRGKEQSWCFLVYSDGLTEDAKERLKALLATTDGFALAEEDLR
                     IRGPGELAGTAQSGYLRLTIADPVRDAKLLEEARAEAFALVEADPGLLSANNAVIREV
                     LSRANPFGEAAAQN"
     gene            complement(2024..>2286)
                     /locus_tag="VFL04_04285"
     CDS             complement(2024..>2286)
                     /locus_tag="VFL04_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838951.1"
                     /translation="KGAVEELKTAEKARETAARKLTAAKKASPKPKTVARKVEKPARL
                     KAVAKPVKKPVAKAKLRAKKLARTPSPKAPAPKSARQARTGR"
BASE COUNT          303 a          846 c          781 g          356 t
ORIGIN      
        1 ctcggccttc gcgaaggagc cgagggtcca cacggtggcc accgtggtcg agcatgagtg
       61 gttcggcttc ggccgcatgc gcaccctcaa gatcagggtc gaggacggct cgggccgggc
      121 ggtcctcgtc tgcttcaacc gtcccttcct cgagaacagc ctgcccgtgg gggccagggt
      181 cctcgtcacg gggaaattcg gcttccgcta cggcgagatc cagtcctcgg ccttcgaggc
      241 cgagcgcctc gacggcgagg gcggggcgaa ggaggggatc ctccccgtct acgccctcac
      301 cgacggcctg gcccagggcc ttgtgaggcg cctcgccaag cgggccatcg tggagtacgg
      361 ttccaaggtc gaggacgagc tgcccccgcc cctgatggtc cgccgcggac ttctgcccaa
      421 ggccgaggcc ctcgcgaagg tccacttccc gcccagcctg gctgagctcg agcgcgcgcg
      481 ctcgagcctc atcttcgagg agctcttcta tttccagctc gccgtggcgc gccgcgccat
      541 gaggcgccgc gaggccgccg tgccgcgagc cgcggtcagg ggcctcctca aggcccgcct
      601 cgtcgagcgt ctgcccttcg cgctcacggc cgaccagctg aaggcctcgg acgagatcgc
      661 ggcggacatg ggctcgccga ggccgatggc ccgcctcctc cagggcgacg tcggctcggg
      721 caagaccctt gtggccttcc tcgcctgcct cgacgcgatc gaggccgggg gccaggccgc
      781 cctcatggcc ccgaccgagc tcctcgcccg ccagcacgcc tccaccgcag cccgcttcct
      841 cgagcccctg gggatccgcc tcgccttcct ctcgggcaac gtcgaggacg cggcccgccc
      901 tcccctcctc gcggccctca aggccggcga ggtcgacctc ggcctcggca cccaggccct
      961 cttctccgag gaggtcgagt accggaacct caggctcgcc gtcgtcgacg agcagcaccg
     1021 cttcggcgtg ctccagcgcc tcgccctcgg gaggaagggg cccctgcccg acctcctcat
     1081 gatgaccgcc acccccatcc cgaggaccct cgccctcacc gtcttcggcg acctcgaggt
     1141 ctcggccatc cggacgatgc ccccggggcg gaaaagcgtg atcacccatc tcgccaaggc
     1201 gggcaacgag aagcgggtct acgagttcgt ccgaggcctc ctggcccagg gcagacaggc
     1261 ctatttcgtc tatccgctga tcgggggctc ggagcggcca tcgtcgaagg agctcaagaa
     1321 cgccgaggcc atggcggagc atctcaggac cgcggtcttc cccgagtaca gcctcggcct
     1381 catccactcg aggctcaagg aggaggagaa gcgctccaca atggaggcct tcgcgagggg
     1441 ccagaccagg atcctcgtcg cgacgagcgt cgtcgaggtg ggggtcgacg tcccgaacgc
     1501 cacggccatg gtgatcgagc acgccgagcg cttcggcctt gccgccctcc accagctgcg
     1561 gggccgggtg ggccggggca aggagcagtc ctggtgtttc ctcgtctact cggacggcct
     1621 gaccgaggac gccaaggaga ggctcaaggc cctcttggcc acgaccgacg gtttcgccct
     1681 cgccgaggag gacctccgga tcagggggcc gggggagctc gcggggacgg cccagtccgg
     1741 ctatcttcgg ctgaccatcg ccgatcccgt gagggacgcg aagctcctcg aggaggccag
     1801 ggccgaggcc ttcgccctcg tcgaggccga ccccggcctg ctctcggcca acaacgccgt
     1861 gatcagggag gtcctttccc gggcaaatcc ttttggtgag gctgcggcgc agaactgaag
     1921 ctcaggcgga gcccgagcgg ggcggagcct gagccaggcc cggcctgcgc gacgccccgg
     1981 cccccgcggg gccctgcgtc ctagacgggg cgcggctcga ggcctagcgg ccggttctgg
     2041 cctggcgggc gctcttcggg gccggggcct tggggctggg ggtccgcgcg agcttcttgg
     2101 cgcggagctt ggccttggcg acgggcttct tgaccggctt cgcgacggcc ttgagcctcg
     2161 ccggcttctc caccttcctg gcgaccgtct tgggtttggg gctcgccttc ttcgcggcgg
     2221 tgagcttcct ggccgccgtc tcgcgcgcct tctcggcggt cttgagctcc tcgactgcgc
     2281 ccttgc
//
LOCUS       DASSQV010000250         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2299440, whole genome shotgun sequence.
ACCESSION   DASSQV010000250 DASSQV010000000
VERSION     DASSQV010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2299440"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VFL04_04290"
     CDS             <1..914
                     /locus_tag="VFL04_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7838952.1"
                     /translation="RRSLSIAILVSAGIAALFGIVLSIQITGPLAALDGAAKRIAAGD
                     LSVTVQVRGEDEVASLSVSFNAMTGELKRLDEAKRRIIADSAHELRTPVTLIRGLLEG
                     VIDGVFPLDMDMIRSVHDETLRLSRLIDMLHELELIDSGELRLSLDEMDLIPALEKAA
                     SLFGPSAGEKGICIEVGRGDPGGVRALVDVLRFDEVLYNLIGNAIKYSPRGGKVLCSA
                     RRAGASVLIAIEDSGPGIPGPEREKVFERFYRMDKSRAQDSGGRGLGLSIAYEIVKAH
                     GGSIGIEDSPLGGASFVVSLPAMPGRA"
     gene            complement(951..>2199)
                     /gene="chrA"
                     /locus_tag="VFL04_04295"
     CDS             complement(951..>2199)
                     /gene="chrA"
                     /locus_tag="VFL04_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HET7838953.1"
                     /translation="GSWCSGSGDRVGADPKGEARGSEDPRGRKSARLLRLIGYFLRLG
                     TLGFGGPVALVGYMHRDLVEARGWIGEDDYRLALALAQIMPGPLAAQTAIGICYFDSG
                     LAGATLGGIAFVLPSFLIVVAISILYVAFGGSWWMQSLFYGIGAAIIAIVAIAALKLA
                     RTTNRRDPLSWGIFAVLMATTYFTRTELAWVFIVAGLVALLVRAPPRWLCGQPRQLAL
                     SPVPAAIGATALATPLVAGSSLLTLFLFFLKAGAFVFGSGLAIVPFLHQGVVEQYRWL
                     SERQFLDAVAVAMITPGPVVITVVFIGYIVARLPGALVSAIGIFLPVYLFTIVPALWF
                     RRHRDDPHLKAFVQGVTAAATGAIAGAVVILGEKAISDIPTAAIALVSLAMLWFLKLP
                     EPILVLVAGAVGVGLRIIGGHVA"
BASE COUNT          391 a          731 c          785 g          292 t
ORIGIN      
        1 tcaggaggtc actgtccatc gcgatcctcg tctcggcggg catcgcggcc ctcttcggca
       61 tcgtgctctc gatccagatc acggggcccc tggcggccct cgacggggcc gccaaaagga
      121 tcgcggccgg cgacctctcg gtgacggtgc aggtcagagg ggaggacgag gtcgcgagcc
      181 tgtcagtttc gttcaacgcg atgaccgggg agctgaagcg gcttgacgag gcgaagcgca
      241 ggatcatcgc cgactcggcg cacgagctca ggaccccggt caccctcatc cgcggcctcc
      301 tcgagggcgt gatcgacggg gtcttccccc tcgacatgga catgatcagg agcgtccacg
      361 atgagaccct gcgtctgtcg aggctcatcg acatgctcca cgagctcgag ctcatcgact
      421 cgggcgagct gaggctgtcc ctcgacgaga tggacctcat cccggccctc gagaaggcgg
      481 ctagcctgtt cggaccatcg gccggggaga aggggatatg catcgaggtc ggccgcggcg
      541 atcccggcgg ggtccgggcc ctggtcgacg tcctgcgttt cgacgaggtc ctctacaacc
      601 tgatcgggaa cgcgatcaag tacagcccca gggggggcaa ggtgctctgc agcgcccggc
      661 gcgcgggagc ctcggtcctc atagccatcg aggactcggg cccgggcatc cccgggcccg
      721 agcgggagaa ggtcttcgag cgcttctacc gcatggacaa gtcccgggcc caggactcgg
      781 gaggccgcgg actcggcctc tcgatagcct acgagatcgt caaggcccat ggcgggagca
      841 tcgggatcga ggactcgccg ctcggcggcg cctccttcgt cgtgagcctc ccggccatgc
      901 cggggcgggc ctaggccctc ttcctccagt acgcgaagta gggcgcgacc tcaggccaca
      961 tggccgccga tgatccgcag ccccaccccg accgcgccgg cgacgaggac gaggatcggc
     1021 tcgggcagct tgaggaacca cagcatcgcg aggctcacga gggctatcgc cgccgtgggt
     1081 atgtcgctga tggccttctc cccgaggatg accacggccc ccgcgatagc gccggtcgcc
     1141 gctgcggtca cgccctggac gaaggccttg aggtgggggt catcgcggtg cctccggaac
     1201 cagagcgccg gaacgatcgt gaagaggtag accggcagga agatgccgat cgccgagacg
     1261 agggcgcccg gcagccgggc cacgatgtag ccgatgaaga ccacggtgat gaccacgggc
     1321 cccggcgtga tcatcgcgac ggcgacggcg tcgaggaact ggcgctcgct gagccagcgg
     1381 tactgctcga cgaccccctg atgcaggaag ggcacgatcg cgaggccgct gccgaagacg
     1441 aaggccccgg ccttgaggaa gaagaggaac agggtgagga ggctcgaccc ggccaccagg
     1501 ggcgtggcca gggccgtcgc cccgatcgcg gccgggaccg gtgagagcgc gagctgacgc
     1561 ggctgtccgc aaagccacct tggaggggcc ctgaccagga gggccacgag gccggcgacg
     1621 atgaagaccc aggcgagctc tgtcctcgtg aagtaggtag tggccatgag gacggcgaag
     1681 atcccccagg agagcggatc gcgccggttc gtcgtcctcg cgagcttgag ggccgcgatg
     1741 gccactatcg cgatgatcgc agcgccgatc ccgtagaaca gcgactgcat ccaccaggag
     1801 ccgccaaagg ccacatagag gatcgagatg gccacgacga tgaggaagga cggcaggacg
     1861 aaggcgatgc cgccgagggt cgccccggcc aggcccgagt cgaagtagca gatgccgatg
     1921 gccgtctggg ccgcgagggg gccgggcatg atctgggcga gggcaagggc cagccgatag
     1981 tcgtcctccc cgatccaccc gcgggcctcg acgaggtcgc ggtgcatgta gccgaccagg
     2041 gcgacggggc cgccgaagcc cagggtgccg agcctcagga aatagccgat gagccgcagg
     2101 agtcttgcgg acttccgccc gcggggatcc tcggagcccc gggcctcccc cttcggatca
     2161 gctcccaccc ggtcccccga gcccgaacac caggatccg
//
LOCUS       DASSQV010000251         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1179174, whole genome shotgun sequence.
ACCESSION   DASSQV010000251 DASSQV010000000
VERSION     DASSQV010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179174"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1265)
                     /locus_tag="VFL04_04300"
     CDS             complement(183..1265)
                     /locus_tag="VFL04_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838954.1"
                     /translation="MEEHGKRRLAAAGAVVAGIAYFLICAIPLPRELVLVPVWARDLS
                     GAGIPAAAGAKPAAEATTMPFTLGQRFGYFDSQGRILFAAPRPYGVAVSRQSFTTYER
                     LSTSFSLRSPGGPELLRCDLPGYPFMAAGRLFVVGPNQSTVSEIGGDGKPKWTYEFPS
                     IVTAFGASPGLAAFGLLDGTMIGLDASGAEALHFSPGGSRIAGIYGLAVAPDGLMVAA
                     IAGLDKQRLLVLERRSSAFRVTYHRWLDSDFRRPVALSFTSDGRKLVYEAPGGAGVYD
                     RATRSESLVAVPGPEGLGLSVGAEGLLVLIGQGTDHKRLVLAAAPDRRIADLPVFAKE
                     TFLECETSSLFLGMDDKIVRLDLREE"
     gene            1352..1753
                     /locus_tag="VFL04_04305"
     CDS             1352..1753
                     /locus_tag="VFL04_04305"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET7838955.1"
                     /translation="MDQDKLFAMALEASHAAYAPYSKFRVGAALLCADGEVFTGVNVE
                     NRSFGLTVCAERSALSAAVSAGKRDFLGIVVATPDADYPVSPCGACRQVLSEFLPSSA
                     PVVFGCAADRRVVTTMGELFPSDALHELKRR"
     gene            complement(1809..2615)
                     /locus_tag="VFL04_04310"
     CDS             complement(1809..2615)
                     /locus_tag="VFL04_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838956.1"
                     /translation="MGKITSEQRNRYFEKVQEHRKSIEAGLAKERTLLQLVSSDESGA
                     GYKRLALAESTLDLCSWYILVSTLSVSMLGMKNEEYLLEARKTLVRSIRYLEETVTAY
                     LDAPFSDYEAKLEEIKDFDSDNRFRLVRKMGFAIQSLEDAFGENSKYAASFIEVWGRF
                     GAVAKNLINLKTAVPDMDFSSPIRAVVMSHIGLVKSLFQRSADKYREQYEHHTHKTDD
                     FKRAILFLQALRRIHVVLSERDEAETVKRRIEIWTTKLEADQKKAEEAKK"
     gene            complement(2615..>3429)
                     /locus_tag="VFL04_04315"
     CDS             complement(2615..>3429)
                     /locus_tag="VFL04_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET7838957.1"
                     /translation="ERLGEVETRLADIHKLKKKYGPTVEAVLARLEEDRDRLARLETW
                     EEDKGEIERRIADMEEKVLAEALAISDKRRSGAGPLQARIEAIVRTLGMPNARFLVRV
                     GRKEGEAGKAVVGPHGIDEIEFLFAPNPGEPAKALARIASGGELSRVALAAKTVLAGT
                     DDVDTLIFDEVDTGIGGEVAVAVGEHLKQLGKAKQVLCITHLASIAVRADNHYRVEKE
                     VSGGRTSTRLSRMEGKQRAEEIARMLAGDPREEASIAHAVELLRKHGNWRDG"
BASE COUNT          524 a         1116 c         1200 g          589 t
ORIGIN      
        1 agtcccgaag gtggcggcga tccggacggg ggacaggggc cagtcgaggg cccaggcggc
       61 caggccgccg aaggccaggg cggcgagggc gatggacgcg gccctggccg ggccgagccg
      121 catgctggaa aagccggtga cacgcctcgg ggcctggccc cctctccgac ttgtctcgcc
      181 catcattcct ccctaaggtc caggcgcact atcttgtcgt ccatcccgag aaagagggac
      241 gaggtctcgc attcgaggaa ggtctccttc gcgaacacgg gcaggtcggc gatcctccgg
      301 tcgggcgcgg cggcgaggac gaggcgctta tggtcggtgc cctggccgat gaggacaagg
      361 aggccctcgg cgccgacgga caggccgagc ccctcggggc ccggcaccgc gaccagggac
      421 tcgctccgcg ttgcgcgatc gtagaccccc gccccgcccg gggcctcgta gaccagcttc
      481 cggccgtcgg aggtgaagga gagggcgacg ggcctgcgga agtccgagtc gagccagcgg
      541 tggtaggtca cgcggaaggc cgaggacctc cgctcgagga cgaggaggcg ctgtttgtca
      601 aggccggcga tggccgccac catgaggccg tcgggggcca cggcgaggcc gtagatgccc
      661 gcgatgcgcg atcctcctgg ggagaagtgg agggcttcgg cgcccgatgc gtcgaggccg
      721 atcatcgtgc cgtccaggag gccgaaggcg gcgaggccgg ggctggctcc gaaggccgtc
      781 acgatcgagg ggaactcata ggtccatttc ggcttgccgt cgcctccgat ctcggagacc
      841 gtggactggt tcgggcccac gacgaagagc ctgccggcgg ccatgaaggg gtagcccgga
      901 agatcgcagc gcaggagctc gggcccgccg ggtgacctca gggagaagct cgtggagagg
      961 cgctcgtagg tcgtgaagga ctggcgggag accgccaccc cgtagggccg tggggccgcg
     1021 aacaggatgc ggccttggga atcgaaatag ccgaagcgct ggccgagggt gaatggcatg
     1081 gtcgtggcct cggcggccgg cttggccccg gccgccgcgg ggatgcccgc gccggacagg
     1141 tcccgggccc agacggggac gaggacgagc tcgcgcggca agggaatggc gcagatgagg
     1201 aaatacgcga tgcccgcgac gaccgctccc gcggccgcca ggcgccgttt gccatgctct
     1261 tccatattcc ccttcgctcc ccgcccttca cgcgtcggtg tcgatcgtat atagttgctc
     1321 tctcatctgt caactcggtg tgaggacggc aatggatcag gacaagcttt tcgcgatggc
     1381 cctcgaggct tcgcacgctg catatgcccc ctactcgaag tttcgcgtcg gcgcggccct
     1441 gctctgcgcc gacggcgagg tcttcactgg ggtcaacgtg gagaaccgct ctttcgggct
     1501 aaccgtgtgc gcagagcgct cggccctctc cgcggccgtg agcgccggca aacgcgattt
     1561 cctcgggata gtggtggcga cccccgacgc cgactacccc gtgagcccct gcggggcctg
     1621 cagacaggtg ctatcggagt tccttcccag ttcggccccc gtcgtcttcg gctgcgccgc
     1681 ggacaggagg gtggtgacca caatgggaga gctgttcccc agcgacgccc ttcacgagct
     1741 gaagcgtcgc tagcgacgcc ctccatgagc tgaagcagcg ccactaaccg gggaggccga
     1801 ggcccgggct acttcttggc ctcttcggcc ttcttctggt cggcctcgag cttggtcgtc
     1861 cagatctcga tccgccgctt gaccgtctcg gcctcatccc gctccgaaag gaccacgtgg
     1921 atcctgcgga gggcctggag gaagaggatg gcgcgcttga agtcgtcggt cttgtgggta
     1981 tggtgctcgt actgctcgcg gtacttgtcg gcgctgcgct ggaagaggct cttgaccagg
     2041 ccgatgtggc tcatgacgac ggcgcggatc ggggagctga aatccatgtc gggcacggca
     2101 gtcttcaggt tgatgaggtt cttggcgacg gcgccgaagc ggccccagac ctcgatgaag
     2161 gaagccgcgt acttggagtt ctcgccgaag gcgtcctcga gggactggat cgcgaagccc
     2221 atcttcctga ccagccggaa gcggttgtcg ctgtcgaaat ccttgatctc ctcgagcttg
     2281 gcctcgtagt ccgagaaggg ggcgtcgagg taggcggtca cggtctcctc gaggtagcgg
     2341 atggagcgga cgagggtctt gcgggcctca aggaggtact cctcgttctt catgccgagc
     2401 atcgagaccg aaagggtgct cacgaggatg taccaggaac agaggtcaag ggtgctctcg
     2461 gccagggcga gccgtttgta gccggccccg ctctcgtcgc tcgagacaag ctggaggagg
     2521 gtcctctcct tggccagccc ggcctcgatc gacttgcggt gctcctgcac cttctcgaaa
     2581 taacggtttc tctgttccga ggtgattttt cccatcagcc gtcccgccag ttaccgtgct
     2641 tccggaggag ctccacagca tgcgcgatgg aggcctcctc gcgggggtcg cccgcgagca
     2701 tccgggctat ctcctcggcg cgctgcttcc cctccatgcg cgaaagccgc gtggacgtgc
     2761 gccccccgga gacttccttc tcgaccctgt agtgattatc ggctcgaacc gctatggagg
     2821 cgagatgggt aatgcaaagg acctgtttcg ccttcccgag ctgtttcagg tgctccccga
     2881 cggccacggc gacctcgccg ccgattcccg tgtccacctc gtcgaagatc agggtgtcga
     2941 catcgtcggt gccggccagg acggtcttgg cagccagggc cacgcgggag agctccccgc
     3001 ccgaggctat ccgcgcgagg gccttcgccg gctcgccggg gttgggggcg aagaggaact
     3061 cgatctcgtc gatgccatgg ggcccgacga cggccttccc ggcctcgccc tccttcctcc
     3121 cgacccttac caggaaacgg gcgttgggca tgccgagggt cctgacgatc gcctcgatcc
     3181 gggcctggag ggggccggcg cccgagcggc gcttatcgga gatcgcgagg gcctcggcca
     3241 ggaccttctc ctccatgtcg gcgatccggc gctctatctc gcccttgtcc tcctcccagg
     3301 tctcgagccg ggccagacgg tcgcggtcct cctcgagccg ggccagtacg gcctccacgg
     3361 tggggccgta cttcttcttg agcttgtgga tgtcggcgag cctggtctcg acctcgccga
     3421 ggcgctcgg
//
LOCUS       DASSQV010000252         4339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1802974, whole genome shotgun sequence.
ACCESSION   DASSQV010000252 DASSQV010000000
VERSION     DASSQV010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802974"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1150)
                     /locus_tag="VFL04_04320"
     CDS             complement(29..1150)
                     /locus_tag="VFL04_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838958.1"
                     /translation="MPELLASLLGAGHERALAARGSLPGLALAAKNEARRSAGLPPYA
                     SVEAWLAELAPRTVCLSLLAGSGSRWLRSLEAARARVAAGTGGSASSLDAQASAFDPA
                     RPRGLFPVRNLLVPGGPRIAVAAYALAAVKGLGRHVLVVRGWEAEIDAEVLAPLGIGS
                     GARAYQTQGAELGKPLGHGDAAWQARGLWSKADYVITNFGGDANSRRTAINSLLALDA
                     FGAPGGAGADLIIPVARIANPAYPVALDAEGIPRGFGHAKLQGQALGGGSGYTNVGLR
                     VYRAGALLEKVEAFRSRHWVEGSGYAIPGNDPAGHEFALDNVDAALASEGRARILAAS
                     LPQELTPAKSLEEVPAFEEAVAAVVADDREEGKHGATEY"
     gene            complement(1143..2069)
                     /locus_tag="VFL04_04325"
     CDS             complement(1143..2069)
                     /locus_tag="VFL04_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HET7838959.1"
                     /translation="MVIPEYPNFAPVSLEMRDELYPSLNMIKDGISEFTFSNLYLFRH
                     TYGYRVSRIPGKTFIVDGSKLGKSFFYAPCALPEPAILDELMAGHDYLKNLSETQADA
                     HRAALEMRGYSVVEDRDNFDYLYLRTDLAELTGQTYHKKRTLVNAFLSSYEYEQKPLR
                     RDNLADAIAVLDEWRESKGFEGDYKASREALETFELLGMRGCVYYVAGSPAGWCLGEP
                     LARGRMFAVHFEKAIERYKGIYQFINQAFAQALPAHYITINREQDLGDEGLRQAKMTY
                     RPMGFVNKYRVIRTDRSMAAPSNPEIADLSNA"
     gene            complement(2172..>4339)
                     /locus_tag="VFL04_04330"
     CDS             complement(2172..>4339)
                     /locus_tag="VFL04_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HET7838960.1"
                     /translation="GIVYVGTWGGGLLEYETATGRFTNYRASGGVGSLSHDVVYSILR
                     DSSGELWFGTNGGGIDKLSRARRSYGALEASAQAFPKGKVYAIDLDSRGELWVGVYNG
                     GIAVRSKETGAWRSYRKSPGRPRSLPNDIVNFIHEDRFGRMWAGTNDGLARLDPRRGD
                     FDVYRPGPDKARSLSSEVIYAMAEDPEGALWIGTFRSGLDRLDPVTGTVTHFPHDPNE
                     PSSLSDNLVFALEYDSRGRLWVGTNNGLNRLTEKGFVRYLYDPAKRAGISNDSIRTIF
                     RDSLGVLWFGTAGGGLMRYEPETDSFMTYTKKEGLPSNTVVRILEDSQGELWIATQSG
                     LAIYDRKTGTFRGLTVYNDLRDREFYTGAVRGPDGSLYFGALDGIYRFDPGRYEYNTH
                     RPPVVLTYLQSAGRPKPIPVAAPRLRSLELAWRDNSVEFGFAALDFRDPDRNQYAYRL
                     EGFDHDWISAADRRWASYTNLPGGDYVFRVKASNNDGLWNEEGLALPVHVGWRPWTSP
                     LAFVLYAVLVALLASLLSTRATRKALASTKAALVQASLRCNEVGSQLERLEAYDQLTG
                     TLDRRGLEERLEADFARARAARESVAALIVEVDRFSAYNDSRGRQAGDECLRRIAACL
                     IASLERATDSITRYGGAKFLVLIANADVEMATEVAQRLRRSVEALAIAHDGGSGLVTV
                     SIGCAALRPELGLSSAALLASADRALYQAQERGGNAVAS"
BASE COUNT          620 a         1412 c         1616 g          691 t
ORIGIN      
        1 ccatgccgga ctcccctccc tgccctctct agtactccgt ggctccgtgc ttgccttcct
       61 cccggtcgtc ggccacgacg gccgccaccg cctcctcgaa ggccgggacc tcctcgaggc
      121 ttttcgcggg cgtgagctcc tggggcaggg aggcggcgag gatcctcgcc ctcccctcgg
      181 aggcgagggc ggcgtcgacg ttgtcgaggg cgaactcgtg gccggcgggg tcgttgccgg
      241 ggatggcgta gcccgagccc tcgacccaat gcctcgacct gaaggcctcg accttctcga
      301 ggagggcgcc ggcccggtag actctgagcc ccacgttcgt gtagccagag cctcccccga
      361 gggcctggcc ctggagcttg gcgtggccga agcccctcgg gatgccctcg gcgtcgaggg
      421 ccacgggata ggcggggttc gcgatcctcg cgacggggat gatgaggtcg gccccggccc
      481 cgccgggggc gccgaaggcg tccagggcga ggagggagtt gatggccgtc ctacgcgagt
      541 tcgcgtcgcc gccgaagttg gtgatgacgt agtcggcttt cgaccagagg cccctcgcct
      601 gccaggccgc gtcgccgtgg cccaggggct tcccgagctc ggcgccctgg gtctggtacg
      661 cgcgcgcccc gcttccgatc ccgagggggg cgaggacctc ggcgtcgatc tcggcctccc
      721 agcccctgac gacgaggacg tggcggccga ggcccttcac cgccgcgagg gcgtaggcgg
      781 cgacggcgat tcgcggcccg cccggaacca ggaggttccg cacggggaag aggccgcggg
      841 gccgcgcggg gtcgaaggcc gaggcctggg cgtcgaggct cgaggccgag ccgccggtgc
      901 ccgccgcgac acgggccctc gcggcctcga gggacctcag ccagcgcgag cccgagccgg
      961 ctaggaggct gaggcagacc gtcctcggcg cgagctctgc gagccaggcc tccacggagg
     1021 cgtagggcgg aaggccggcc gagcggcggg cctcgttctt ggcggcgagg gccaggccgg
     1081 ggaggctgcc gcgcgcggcg agggccctct cgtgccctgc cccaaggagg gaggcgagga
     1141 gctcaggcat tcgagaggtc ggctatctcc gggttcgaag gggccgccat ggagcggtcg
     1201 gtcctgatga cccggtactt gttcacgaag cccatggggc ggtaggtcat cttggcctgg
     1261 cggaggccct cgtccccaag gtcctgttcg cggtttatgg tgatgtagtg cgctggcagg
     1321 gcctgggcga aggcctggtt tatgaactgg tagatgccct tgtaacgctc gatggccttc
     1381 tcgaagtgga cggcgaacat cctcccgcgg gccaggggct cgccgaggca ccagcccgcc
     1441 ggggagcccg cgacatagta gacgcagccg cgcatgccca gaagctcgaa ggtctcgagg
     1501 gcctcgcgcg aggccttgta gtcgccctcg aagcccttgg actccctcca ctcgtcgagc
     1561 acggcgatcg cgtcggccag gttgtcgcgc cgcaggggct tctgctcgta ctcgtaggag
     1621 ctgaggaagg cgttcacgag ggtgcgcttc ttgtggtagg tctggccggt tagctcggcc
     1681 aggtcggtcc tgaggtagag gtagtcgaag ttgtcgcggt cctcgacgac gctgtagccc
     1741 cgcatctcca gggccgcccg gtgcgcgtcg gcctgggtct cggagaggtt cttgaggtag
     1801 tcgtggccgg ccatgagctc gtcgaggatg gcgggctcgg gcagggcgca gggggcgtag
     1861 aagaaggact tcccgagctt ggagccgtcg actatgaagg tcttccctgg tatccgcgag
     1921 acccggtagc cgtaggtgtg ccggaaaagg tagaggttcg agaaggtgaa ctcggagatg
     1981 ccgtccttga tcatgttgag cgaggggtag agctcgtcgc gcatctcgag cgaaaccggg
     2041 gcgaaattcg ggtattctgg gattaccatg ccctacatga tacgaaaagc actatatgcg
     2101 aggcaaggcg tgcctcgaag cttgttggcg accctgaccg gccccaccaa caggcttcga
     2161 ggcactgtct tctacgaggc caccgcgttc cccccgcgct cctgggcctg gtagagggcg
     2221 cggtcggcag aggcgagcag ggccgccgag gagaggccca gctcggggcg cagggcggcg
     2281 cagccgatgc tgaccgtcac cagcccggag cccccgtcat gggcgatcgc gagggcctcg
     2341 accgagcgcc tgagccgctg ggcgacctcg gtggccatct cgacgtcggc gttggcgatg
     2401 aggacgagga atttcgcccc cccgtagcgc gtgatcgaat cggtcgcccg ctcgagcgag
     2461 gcgatgaggc aggccgcgat cctgcggagg cactcgtccc cggcctggcg gccccggctg
     2521 tcgttgtagg ccgagaagcg gtcgacctcg acgatgaggg cggccacgct ctcccgggcg
     2581 gccctggccc tggcgaaatc ggcctcgagc ctctcctcga ggcccctgcg gtcgagggtc
     2641 ccggtcagct ggtcgtaggc ctcgagccgc tcgagctggg agcccacctc gttgcacctg
     2701 aggctggcct ggacgagggc tgccttggtc gaggccaggg ccttccgggt ggccctggtt
     2761 gagaggaggg aggccaggag ggcgacgagg acggcgtaga ggacaaaggc gagggggctc
     2821 gtccatggcc tccagcccac gtggacgggc agggccaggc cctcctcgtt ccagagcccg
     2881 tcgttgttcg aggccttcac cctgaagacg tagtcgcccc cggggaggtt ggtgtagctg
     2941 gcccagcggc ggtcggccgc cgagatccag tcgtggtcga agccctcgag gcggtaggcg
     3001 tactggttcc ggtcgggatc gcggaagtcg agggcggcga agccgaactc gaccgagttg
     3061 tcccgccagg cgagctcaag gctccgcagc cgcggggccg ccacggggat cggcttcggc
     3121 ctccccgcgc tctgaaggta ggtgaggacg acgggggggc ggtgggtgtt gtactcgtag
     3181 cgccccgggt cgaagcggta gatgccgtcg agggcgccga aatagaggct gccgtcgggg
     3241 ccgcggaccg ccccggtgta gaactccctg tcccgcaggt cgttgtacac ggtgaggccg
     3301 cggaaggtgc cggtcttgcg gtcgtagatc gccagccccg actgggtggc gatccagagc
     3361 tcgccctggg agtcctcgag gatcctgacc acggtgttgc tcggcaggcc ttccttcttc
     3421 gtgtaggtca tgaaggagtc ggtctcgggc tcgtagcgca tgaggccgcc ccctgccgtg
     3481 ccgaaccaga gcaccccgag cgagtccctg aagatcgtcc gtatggagtc gttggagatg
     3541 cccgcgcgct tggccgggtc gtagaggtag cgcacgaagc ccttctccgt gaggcggttg
     3601 aggccgttgt tcgtccccac ccagagcctc cccctcgagt cgtactcgag ggcgaacacg
     3661 aggttgtccg agagcgagga tggctcgttc gggtcgtggg gaaagtgggt gactgtcccc
     3721 gtcacgggat cgagccggtc gaggcccgag cggaaggtcc cgatccagag cgcgccctcg
     3781 gggtcctcgg ccatcgcgta gatgacctcg gaggagaggc tcctcgcctt gtcgggcccc
     3841 gggcggtaca cgtcgaagtc cccccgccgg gggtcgaggc gggcgaggcc gtcgttggtg
     3901 cccgcccaca tgcgtccgaa gcggtcctcg tggatgaagt tgacgatgtc gttcggcagg
     3961 gagcggggcc tgccggggct cttgcggtag gacctccagg ccccggtctc cttgctccgc
     4021 accgcgatgc cgccgttgta gaccccgacc cagagctcgc cgcgcgaatc gaggtcgatg
     4081 gcgtagacct tccccttggg gaaggcctgg gccgaggcct cgagggcgcc gtagctcctc
     4141 ctcgccctgc tcagcttgtc gatccccccg ccgttcgtgc cgaaccagag ctcgcccgag
     4201 ctgtcgcgga ggatggagta gaccacgtca tgggagagcg agcccacgcc ccccgaggcg
     4261 cggtagttgg tgaaacggcc cgtggccgtc tcgtactcga ggaggccccc gccccaggtc
     4321 ccgacataga cgatccccg
//
LOCUS       DASSQV010000253         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1428405, whole genome shotgun sequence.
ACCESSION   DASSQV010000253 DASSQV010000000
VERSION     DASSQV010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428405"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFL04_04335"
     CDS             complement(<1..838)
                     /locus_tag="VFL04_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838961.1"
                     /translation="MRAAFPANAERWFSPWTRADVLVLVLLLGFSLLARLATLEPVPT
                     GLEERGPWLAAKCLRLGLPLPELAPGGASLGLILPGYVGQLLFGLHPNVYYLAPVAGS
                     LLLVVLVFSLCRRLRGRLGGFLGGLAAALLCVLGRGHPATAPLFPAIVAAILLLIMRY
                     LMDEERRFPGLGFSALMLSLACEMDRGGFVLLAVCLVALPAYRARAPHVLGFGLVFLG
                     SWLAEAAAIAAFGLGPAPSFPKPPAWSGGAILGPVLAAALVPALVALVLAAVGLGEKA
                     AER"
     gene            1061..2425
                     /locus_tag="VFL04_04340"
     CDS             1061..2425
                     /locus_tag="VFL04_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7838962.1"
                     /translation="MSEPSAVPAEAVRDLAEELLGFVRVPSVSSDPERRDDCRGAAAW
                     LRDRFEAAGAAVARLIETPLHPAVFASFPPPPGSPTILVYGHYDVQPAEASAWTSPPF
                     EPEIRNGRVYGRGSSDDKGNVLAALAAVRSLKAGPDGLGIGLKFLIEGEEEIGSPHLD
                     ALLAEHRRLLACDAAFCADGGMFGESSCSIGVSARGGFVLRLWVKGPASDTHSGLVGG
                     AVRNPIHELSALIASLHDGEGMVAVEGFYDRVRPLEAEERLELARLPFDEELFVAANG
                     PLHGEREYSVPERIGARPALDLIAFHGGGGAGGEGVTAIVPSKAWAVLMCRTVADQDP
                     WVVREQVKQHLKKLMPEGLSYGFEEKDCFWDYRVPEGLPALSSAIRALEEVYGTRPLL
                     CRDGGGLPLRRFQDILGVSPVIFAFSRDSEGAHGKDEHFPLEGMGLAMASWTSLYRIL
                     GRRG"
BASE COUNT          405 a          852 c          902 g          338 t
ORIGIN      
        1 cccgctccgc ggccttctcc ccaaggccga cggccgcgag cacaagggcc acaagggccg
       61 ggacgagggc tgcggcgagg acagggccga ggatggcccc cccgctccag gcggggggct
      121 tcgggaacga gggcgcgggg ccaaggccaa aagcggcgat ggcagcggcc tcggcgagcc
      181 acgatccgag gaagacgagg ccgaagccga ggacatgcgg ggctctggcc cggtaggcgg
      241 ggagggcgac gagacagaca gcaaggagga cgaagccgcc ccggtccatc tcgcaggcca
      301 aggacagcat cagggccgag aagccgagcc ccgggaagcg ccgctcctca tccatcaggt
      361 atcgcatgat gaggaggagg atggcggcta caatggcggg gaagaggggt gccgtggccg
      421 gatggccgcg gcccaggacg cagaggaggg ccgccgccag cccgccgagg aagccgccga
      481 gcctgccgcg cagccgcctg cacaggctga agaccagcac caccaggaga aggctccctg
      541 cgaccggcgc gagatagtag acgttggggt ggaggccgaa gaggagttgc ccgacatagc
      601 cgggcaggat caggccgagg ctggcgccgc ccggggcgag ctcgggcagg ggaagcccga
      661 gcctgaggca ctttgccgcc agccatggcc cccgctcctc gagcccggtc ggcaccggct
      721 cgagggtcgc gagccgggca aggagggaga agccgaggag gaggaccagg acgaggacgt
      781 cggccctggt ccagggcgag aaccatcgct cggcgttggc gggaaaggcg gctctcaatc
      841 cgggtcctcc cttggccgca ggacctctgc agtggaccgc ggcggcagga gtgtatacgg
      901 tctggccgac ggccaccaga gcattcggtc tgcgggcttg gcctgacggt ttcgccccga
      961 gcacgagcgg ccctttcaaa cggggctgga ttatcaaata atcacgaggg gcggctggcg
     1021 cggcagcccg ccggacccct cggggagtgg aggtcgcgcc atgtcagagc cttccgccgt
     1081 gccggccgaa gcggtccggg acctcgccga ggagctcctc ggctttgtcc gggtgccgag
     1141 cgtgagctcc gaccccgagc gccgggacga ttgccgcggg gcggcggcct ggctgcgcga
     1201 ccgcttcgag gccgcgggtg cggctgtggc ccgcctcatc gagacccctc tccatccggc
     1261 cgtcttcgcc tccttcccgc cccctcccgg gtcgccgacg atcctggtct atggccacta
     1321 cgatgtccaa ccggccgagg cctcggcctg gacgagcccc cccttcgagc cagagatcag
     1381 gaacggacgg gtctacggcc ggggcagctc ggacgacaag ggcaacgttc tcgcggccct
     1441 cgcggcggta cgctcgctca aggccgggcc agacggcctc gggatcggcc tcaagttcct
     1501 catcgagggc gaggaggaga tcggcagccc ccatctcgat gccctcctgg ccgagcaccg
     1561 caggctcctc gcctgcgatg cggccttctg cgccgacggg ggcatgttcg gcgagagctc
     1621 ctgttcgatc ggcgtctcgg cccggggagg tttcgtcttg aggctctggg tcaagggtcc
     1681 ggcctcggac acccattcgg gcctcgtcgg aggggcggtg cggaacccca tccacgagct
     1741 ctcggccctc atcgccagcc tccacgatgg cgaggggatg gtggccgtcg agggcttcta
     1801 cgacagggtc aggcccctcg aggccgagga gaggctggag ctggccaggc tccccttcga
     1861 cgaggagctc ttcgtggcag ccaatggccc cctccacggg gagagggaat actcggtccc
     1921 ggagcgcatt ggcgcgaggc cggccctcga cctgatcgcc ttccacggcg ggggcggagc
     1981 cgggggcgag ggagtcacgg ccatcgtccc atccaaggcc tgggccgtcc tcatgtgccg
     2041 caccgtcgcc gaccaggacc cctgggtcgt ccgcgagcag gtgaagcagc acctcaagaa
     2101 gctcatgccc gaaggactct cctacggctt cgaggagaag gactgtttct gggactaccg
     2161 cgtgcccgag ggactgcccg ccctctcctc ggccatcaga gccctcgagg aggtctacgg
     2221 gacgaggccc ctcctctgcc gggacggagg gggtctgccc cttcgccgct tccaggacat
     2281 cctcggcgtg tcgcccgtga tcttcgcctt ctcccgggac agcgagggag cccacggcaa
     2341 ggacgagcat ttccccctcg aaggcatggg cctcgccatg gcctcctgga cctcactgta
     2401 ccggatcctc ggcagacggg gctgaccacc tcgcttctcg ggatattggc acgtttcctg
     2461 ctcatttagg ggtgccacca tctacgcaaa cgaggac
//
LOCUS       DASSQV010000254         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1054979, whole genome shotgun sequence.
ACCESSION   DASSQV010000254 DASSQV010000000
VERSION     DASSQV010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054979"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFL04_04345"
     CDS             <1..200
                     /locus_tag="VFL04_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7838963.1"
                     /translation="EETLTAMTLNGAAALGLADRVGSIEVGKAADLVLLDAPSIRFLA
                     YNVGMNVVLATIKEGEVVYRG"
     gene            184..1887
                     /locus_tag="VFL04_04350"
     CDS             184..1887
                     /locus_tag="VFL04_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7838964.1"
                     /translation="MSTAVEAGARDERPGSRGKGGFSLPIARAIDAAYAGGDHRTRRR
                     AKALLHIDLILGLAVLVYGLGRLVFLPGPVGYVATLSCLPFAISLLLLARGLHLAAST
                     LTIICSWAVVTVLIWVDPLAYPYESFEYGLYLLVVVFETVLVAERPWQEYGMGLLALA
                     SIAVHYVLRVLPLGNPDPQIHPTSNFIIALFMILVAQGLTFVMSRMNREVLAMAEAES
                     ARNAARAAGLAEVLEKSRAGLALGASIISRADSQIQLAIENRTALDQDAAQAARLVAI
                     AADLERASAAVEAEGNAVESALREQEAAGAGIASALEAISGFALSLSDATRERKDRLE
                     GLEAGFTAADAAANQAATSIDAIASRARGLLEQVANVSKVASKTNLLAMNAAIEAAHA
                     GAAGSGFAVVSGEVRKLAEEANTSAKEISKALHEAVSAISLAAERSRSSRASFIAIRE
                     EATSFLRSLDEVFSRVAALEEDVRGIAAAEAGSQSASRRVSAAVSTLRHSDSQSRTGI
                     GEVRTAVAALEAATAGLRASTERIHAEARAIKLLGEENGAHLERMDAEVQKLLRSSTL
                     A"
     gene            complement(1884..>2185)
                     /locus_tag="VFL04_04355"
     CDS             complement(1884..>2185)
                     /locus_tag="VFL04_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838965.1"
                     /translation="DHVATLMLGLAGVVTAVPLLCFASAANRITLTRMGFIQFISPSC
                     QLALGLFVYGERLNPPIAVAFATVIVAVGLYAATRGSRDARGAAGSDDPAALAED"
BASE COUNT          339 a          812 c          722 g          312 t
ORIGIN      
        1 tcgaggagac cctgacggcg atgaccctca acggggccgc ggcccttggc ctggccgaca
       61 gggtcggctc gatcgaggtt gggaaggccg ccgacctcgt cctcctcgac gccccctcga
      121 tccgcttcct tgcctataac gtcggaatga acgtcgtgct cgctacgatc aaggagggtg
      181 aagttgtcta ccgcggttga agcaggggcc cgggacgaaa ggccggggtc caggggcaag
      241 ggaggcttca gcctccccat cgcgagggcc atcgacgcgg cctacgcggg cggcgaccac
      301 aggacgaggc gcagggccaa ggccctcctc cacatcgacc tcatcctcgg cctggccgtc
      361 ttggtctacg gcctcggccg cctcgtcttc ctccctggcc ccgtgggcta tgtggccacg
      421 ctctcctgcc tccccttcgc catttccctc ctcctcctcg cccgagggct ccacctcgcc
      481 gcctccaccc tgaccatcat ctgctcctgg gccgtcgtga ccgtcctcat ctgggtcgac
      541 cccctcgcct acccctacga gtccttcgag tacgggcttt atctcctggt cgtggtcttc
      601 gagaccgtcc tcgtcgccga gcggccctgg caggagtacg gcatgggcct cctcgccctg
      661 gcctcaatag ccgtccacta tgtcctgcgc gtcctcccgc tcggaaaccc cgacccccag
      721 atccacccga cctcgaactt catcatcgcc ctcttcatga tcctcgtggc ccagggactc
      781 accttcgtca tgtcccggat gaaccgcgag gtcctggcca tggccgaggc cgagtcggcg
      841 cgcaacgcgg cgagggccgc cggcctcgcc gaggtcctcg agaagtcgag ggccgggctc
      901 gccctcggcg cgagcatcat ctcgagggcc gacagccaga tccagctcgc aatcgaaaac
      961 cgcacggccc tggaccagga cgccgcccag gccgcccgcc tcgtcgcgat cgcggccgac
     1021 ctcgagcggg cctcggccgc cgtcgaggcc gagggcaacg ccgtcgagtc cgccctccgc
     1081 gagcaggagg cggcgggagc gggaatagcc tcggccctcg aggccatcag cggcttcgcc
     1141 ctctcgctct cggacgccac gcgcgagcgc aaggacaggc tcgaggggct cgaggccggc
     1201 ttcaccgccg ccgacgcagc cgcgaaccag gcggccacct caatcgacgc catcgcctcg
     1261 cgagcccggg gcctcctcga gcaggtggcc aacgtctcca aggtcgcctc gaagaccaac
     1321 ctcctcgcca tgaacgctgc catcgaggcc gctcacgcgg gggcggcggg ctcgggcttc
     1381 gccgtcgtct cgggcgaggt gaggaagctc gccgaggagg cgaacacgag cgcgaaggag
     1441 atcagcaagg ccctccacga ggccgtgagc gcgatctccc tcgcggccga gcgcagccgc
     1501 tcgtccaggg caagcttcat cgcgatcagg gaggaggcca cgagcttcct ccgctccctg
     1561 gacgaggtct tctcccgggt agccgccctt gaggaggacg tgaggggcat cgcggcggcc
     1621 gaggccggct cccagtcggc gagccgcagg gtgtcggctg ccgtctccac cctcaggcac
     1681 agcgactccc agagccgaac gggcatcgga gaggtcagga cggctgtggc cgccctcgag
     1741 gcggcgacgg cgggcctgcg cgcctcgacc gagcgcatcc acgccgaggc cagggcgatc
     1801 aagctgcttg gggaggagaa cggcgcccat ctcgagagga tggacgccga ggtgcagaag
     1861 ctcctgcgct cctcgaccct cgcctagtcc tcggccaggg cagcagggtc gtccgaaccc
     1921 gctgcccctc gggcatccct cgagccccgg gtcgcggcgt agagcccgac cgcgacgatg
     1981 acggtggcga aggccacggc gatgggcgga ttcagcctct cgccgtagac gaagagcccg
     2041 agggcgagct ggcagctcgg cgagatgaac tggatgaagc ccatgcgcgt gagggtgatc
     2101 ctgttggccg ccgatgcgaa gcacagaagg ggcacggccg tgaccacgcc cgcgaggccc
     2161 agcatcaggg tcgccacatg gtcgc
//
LOCUS       DASSQV010000255         4751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_310393, whole genome shotgun sequence.
ACCESSION   DASSQV010000255 DASSQV010000000
VERSION     DASSQV010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310393"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFL04_04360"
     CDS             <1..447
                     /locus_tag="VFL04_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838966.1"
                     /translation="MEGGLKALVEASFGTIWVYEDYFLISTIPGHVIGRQDFGELMMM
                     KLEHYAERPVCAIHDNRHNSMSSFGELTLIDRLCAEYRVIGLVVVVGHPRRVREYMSR
                     RLVMRNTDYRLAVSVEEAFAIAEQIMKASRAGPERAQSGGPPQPRG"
     gene            complement(380..1291)
                     /locus_tag="VFL04_04365"
     CDS             complement(380..1291)
                     /locus_tag="VFL04_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7838967.1"
                     /translation="MSPIRESARARGILLALLSAAGFSTLGLFAKLIFSEGFSVQQAL
                     AWRFTVASLVLWLIIGVASRTGARRPPVGKKLIPVLLLGLLGFAPQAGMYFLTVSILD
                     PGIAGLLLYLYPSFVILLSLAVFRKRPNRIQGLSLALSLAGCALTFFRPGSYPIVGLV
                     LGAVMALFYGSYLVVSERLLEGVDPIRATALIMLVAAASYWVLTFATGTAKLPGTGAS
                     VLGILCVGSIATVLPITTLFAAMKRIGASDTSLVSTLEPVLTIALSAIIIGERLGTAQ
                     AIGGCLILAAVVVLRFEPVPVRPAKLS"
     gene            complement(1288..2820)
                     /locus_tag="VFL04_04370"
     CDS             complement(1288..2820)
                     /locus_tag="VFL04_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838968.1"
                     /translation="MSSTEKKVYDYVPERVRDQLVDVFRKRSREATTADLVAITGLPK
                     TQVEAEVKAVSDEYGARLKVTESGEILYSFPSGMRSRYRGLGPSLRRFWKAFKKGAAA
                     VASFLFKIWIVVMLVGYFVLFLALALLALLLSIAAQSAGRGDSRDDRRGGGLGGMMIA
                     GRLFQTIINIWFYSEMFKTDEERLYRAQKRAARRPLHKAIFSFVFGDGDPDATWDATE
                     KRAVLAFLQSNKGLMTMPEFMAITGLPPLEAEERINTYLYEYEGSPEVSEGGVIYYSF
                     PELLRRKDKADRSLGFSVPMRRIAPFSSNPSKSNTWFCVINAVNFLFGSYFLGSALSS
                     HPLLNILYGPDYAGRLVMTRGWDSFYLMVHQLAGKLGGIADPGLLLGAALGAVPLAFS
                     VFFYAVPAIRSLRLKARNERARLENLRRVAYRAVLDSPNAVRPEAIQPHEDAARPKAA
                     DAAKRVVTELAAWAQAEPKADGSYVFAEVERAKAEAAKVRAGVNLKDYELGGEVFDSG
                     SP"
     gene            complement(2817..4298)
                     /gene="glgA"
                     /locus_tag="VFL04_04375"
     CDS             complement(2817..4298)
                     /gene="glgA"
                     /locus_tag="VFL04_04375"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET7838969.1"
                     /translation="MKILMVTSEAVPFAKAGGLGDAVSALSRSLARAGHDVRIILPRF
                     YCIDRKGLEFIPGPLGVPVGSGEEWAAVYRGTLPGSEVPVYFLEHERFFGRDGIYGDK
                     AEPDYADNPERFAFLSRAAFQLCRKLGWIPDIMHAHDWPSALATVYLRHEERHAEFAK
                     TASVLTIHNLGYQGIYDKASFPYFRLPWELFHGAGFEHFDRVNLLKAGITTADCLTTV
                     SPTYAREIQTPDFGFGLDGLLRFRSPELVGILNGVDLHDWDPSRDRYLSATFSASKME
                     GKDRCKAELQREFGLELDPSRPLVGMVTRLTDQKGVGELFGPLYGSAYRICSDMDLQF
                     AVVGSGESWCEDELRSLSSRLPNFRAKIGYQERMAHLIEGGSDFFLMPSRYEPCGLNQ
                     MYSLHYGTLPIVHRTGGLADTVENYNQETGSGTGFMFDHLTPRSIYDTVGWAVWAWYN
                     RPEHIRAMRTRAMGRTFSWEHSAEEYQKLYDRALVRRQGPEGE"
     gene            4456..>4751
                     /locus_tag="VFL04_04380"
     CDS             4456..>4751
                     /locus_tag="VFL04_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7838970.1"
                     /translation="MAERDKEAAMAIKGEWIRYGDGSGFMALPERAAQPLPGVIVIQE
                     VWGVEEHIEDLTRRIAAAGYAALAPDLYAAGGRRPEALSNPRIAETRAFMATIPP"
BASE COUNT          828 a         1471 c         1725 g          727 t
ORIGIN      
        1 atggagggcg ggctcaaggc cctggtcgag gcgtcgttcg gcacgatctg ggtatacgaa
       61 gactatttcc tcatcagcac gatccccggc cacgtgatcg gccgccagga cttcggcgag
      121 ctcatgatga tgaagctcga gcactacgcc gagcgcccgg tctgcgcgat ccacgacaac
      181 cggcacaaca gcatgagctc cttcggcgag ctcaccctca tagaccggct ctgcgccgag
      241 taccgggtca tcggcctcgt cgtggtcgtg ggccatccga ggagggtccg cgagtacatg
      301 agtcgccgcc tcgtcatgag gaacaccgac taccgcctcg ccgtctccgt cgaggaagcc
      361 ttcgccatcg ccgagcagat catgaaagct tcgcgggccg gaccggaacg ggctcaaagc
      421 ggaggaccac cacagccgcg aggatgaggc aacccccgat ggcctgggcc gtgcccaggc
      481 gctcgccgat gatgatggcc gacagggcta tcgtgaggac gggctcgagg gtgctcacga
      541 gcgaggtgtc cgaggcccct atccgcttca tggcagcgaa gagggtggtg atcggcagga
      601 cggtggcgat gctccccacg cagaggatgc ccaggaccga agcccccgtc cccggaagct
      661 tggccgtgcc cgtggcgaag gtgaggaccc agtagctcgc ggccgccacc agcatgatga
      721 gggccgtggc cctgatcgga tcgaccccct ccaggagccg ctcgctgacc accagatatg
      781 agccgtagaa cagggccatc accgcgccga gaacgaggcc cacaatcggg tagctccccg
      841 gcctgaagaa ggtcagggcg cagccggcga gggagagggc cagggagagg ccctggatcc
      901 ggttcggccg cttccggaag acagccaggg acagaaggat cacaaaggaa gggtagaggt
      961 acagaaggag cccggctatc ccggggtcga ggatgctcac cgtgaggaag tacatcccgg
     1021 cctggggggc gaagccgagg aggccgagga ggaggacggg gatgagcttc ttccccacgg
     1081 ggggccgccg ggcccccgtc ctcgacgcga cgccgatgat gagccagagc acgagggagg
     1141 ccacggtgaa gcgccaggcc agggcctgct ggaccgagaa gccctcgctg aagatgagct
     1201 tggcgaagag gccgagggtg gagaagcccg ccgcggagag gagggcgagg aggatgcccc
     1261 tcgccctcgc gctttccctg atggggctca cgggctgccc gagtcgaaca cctcgccccc
     1321 gagctcgtag tccttgaggt tgacgccggc tcggaccttg gccgcctcgg ccttggcccg
     1381 ctcgacctcg gcgaagacgt agctgccgtc ggccttgggc tcggcctggg cccaggcggc
     1441 gagctcggtc acgacgcgct tggcggcatc ggcagccttg ggcctggcgg cgtcctcgtg
     1501 gggctggatc gcctcgggcc gcacagcatt cggcgagtcc aggacggcgc gataggcgac
     1561 gcggcggagg ttctcgagcc tggccctctc gttgcgggcc ttgaggcgga gcgagcggat
     1621 ggccggcacg gcgtagaaga agaccgagaa ggccaggggc acggccccga gggcggcgcc
     1681 gaggaggagg cccgggtcgg caattccccc gagcttgccc gccagctggt ggaccatgag
     1741 gtagaaggag tcccagcccc tggtcatgac gaggcgtccg gcgtagtcgg gtccatagag
     1801 gatgttaagg aggggatggg aggagagggc gctcccgagg aagtaggagc cgaagaggaa
     1861 gttgacggcg ttgatgacgc agaaccaggt gttggacttg ctcgggttgg agctgaaggg
     1921 ggctatcctc cgcatgggca ccgagaagcc gaggctcctg tcggccttgt ctttgcggcg
     1981 caggagctcg gggaagctgt agtagatcac cccgccctcg ctgacctctg ggctgccctc
     2041 gtactcgtag aggtaggtgt tgatcctctc ctcggcctcg agcggcggca ggcccgtgat
     2101 cgccatgaac tcgggcatgg tcatgaggcc cttgttggac tggaggaagg cgaggactgc
     2161 ccgcttctcg gtggcgtccc aggtggcgtc ggggtccccg tcgccgaaga cgaaggagaa
     2221 gatggccttg tgcaggggtc tgcgggccgc ccgcttctgg gcgcggtaga gcctctcctc
     2281 gtcggtcttg aacatctcgg agtagaacca gatgttgatg atggtctgga agaggcggcc
     2341 tgcgatcatc atgccgccga ggcccccgcc cctcctgtcg tccctcgagt cccccctccc
     2401 cgccgactgg gcggcgatgg aaagcaggag ggccagcagg gccagggcga ggaagaggac
     2461 gaagtagccc acgagcatca cgacgatcca gatcttgaag aggaaggagg ccacggccgc
     2521 cgcccccttc ttgaaggcct tccagaagcg ccggagcgag gggccgaggc cccggtacct
     2581 cgagcgcatg cccgagggga aggagtagag gatctccccc gactcggtga ccttgagcct
     2641 ggccccgtac tcgtcggaga cggccttgac ctcggcctcg acctgggtct tgggcaggcc
     2701 ggtgatggcg acgaggtcgg ccgtggtcgc ctcgcgcgag cgcttgcgga acacgtcgac
     2761 gagctggtcc ctgacccgct cggggacgta gtcgtagact ttcttttcgg tgctgctcat
     2821 tcgccctccg gtccttgcct gcgcacgagt gcgcggtcgt atagtttctg gtactcctcg
     2881 gccgagtgct cccaggagaa ggtcctgccc atggccctgg tccgcatggc cctgatgtgc
     2941 tcgggccggt tgtaccaggc ccagaccgcc cagcccaccg tgtcgtagat ggagcgcggg
     3001 gtcaggtggt cgaacatgaa gcccgtgccc gagcccgtct cctggttgta gttctcgacg
     3061 gtgtcggcga ggcccccggt gcggtgcacg atgggcaggg tgccgtagtg cagggagtac
     3121 atctggttga gcccgcaggg ctcgtagcgg ctcggcatga ggaagaaatc ggagccgccc
     3181 tcgatgaggt gggccatccg ctcctggtag ccgatcttgg cgcggaagtt cgggagcctc
     3241 gaggagagcg agcggagctc gtcctcgcac cagctctcgc ccgagccgac cacggcgaac
     3301 tggaggtcca tgtcgctgca gatgcgatag gcgcttccgt agagggggcc gaagagctcg
     3361 cccacgccct tctggtcggt gagcctcgtg accatgccca ccaggggccg gctcgggtcc
     3421 agctcgaggc cgaactcccg ctggagctcg gccttgcacc tgtccttgcc ctccatcttc
     3481 gaggccgaga aggtggcgct gagataccgg tcccggctcg ggtcccagtc gtggaggtcg
     3541 accccgttga ggatgccgac gagctcgggg gagcggaagc ggaggaggcc gtcgaggccg
     3601 aagccgaagt cgggcgtctg gatctcgcgg gcgtaggtgg gggagacagt cgtgaggcag
     3661 tcggccgtgg tgatgcccgc cttgaggagg ttgacccggt cgaagtgctc gaaaccggcg
     3721 ccgtggaaga gctcccaggg caggcggaaa tagggaaagg aggccttgtc gtagatgccc
     3781 tggtagccca ggttgtggat cgtgagcacc gaggccgtct tggcgaactc ggcatggcgc
     3841 tcctcgtgtc tgaggtagac agtggcgagg gccgagggcc agtcgtgggc gtgcatgatg
     3901 tcggggatcc agccgagctt gcggcagagc tggaaggcag cccgtgagag gaaggcgaag
     3961 cgctcggggt tgtcggcgta gtcgggctcg gccttgtcgc cgtagatccc gtcccggccg
     4021 aagaagcgct cgtgctcgag gaagtagacg gggacctcgg agccggggag ggtccccctg
     4081 tagactgccg cccactcctc gcccgagccc acgggcacgc caagggggcc ggggatgaac
     4141 tcgaggccct tgcggtcgat gcagtagaaa cgcggcagga tgatgcggac atcgtggccc
     4201 gcccgggcga gggagcgcga gagggcggac acagcgtcgc ccaggcctcc tgccttcgcg
     4261 aaagggacag cctcgctcgt gaccatcagt attttcacgg acgcactcct tcactttcgc
     4321 cgaagccggc ctttagactg cccgtcttcc cgcaccagta tacctaggac cgcgggggat
     4381 tgccaacgag gagggcagga cagcccgccc ctctaggaac attttgccgc ccaccgcgta
     4441 acagaagatg ctaaaatggc cgagcgagac aaggaggcgg ccatggcgat caagggcgaa
     4501 tggatacgct acggggacgg atcgggcttt atggcccttc ccgaacgggc tgcccagccc
     4561 ctgcccgggg tcatcgtgat ccaggaggtc tggggggtcg aggagcacat cgaggacctg
     4621 acgaggagga tcgccgcggc cggctacgcg gccctcgccc ccgacctcta cgccgccgga
     4681 ggcaggaggc cagaagccct ctccaatccg cggatagcgg agacaagggc cttcatggcc
     4741 accattcccc c
//
LOCUS       DASSQV010000256         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1428767, whole genome shotgun sequence.
ACCESSION   DASSQV010000256 DASSQV010000000
VERSION     DASSQV010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428767"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1264)
                     /locus_tag="VFL04_04385"
     CDS             complement(158..1264)
                     /locus_tag="VFL04_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7838971.1"
                     /translation="MVLTAGIVLASCAPRQADPVAVKALTPRMGKIADRVEMSGVLVP
                     NKTVTIFPKLVGIARSVAVDTGDRVAEGQVLVQIDAKELLAQLQVAEASVQTVRDQAA
                     QAKVGIESARLNLDMAQKNYDRTKALLDSKSVTQSAADDAQTKLDLAKAAFDNANRQY
                     QTVGGSGLAQAEAQANLIRVQISNSTITSPISGTVVNRNLNLGELTSTSTAIMTIADT
                     GNLKLQGTVPQGDVFHLAVGDKVRIRVDGMPDREYEGTISQLGPIAATTGQYFPVSVG
                     LRNDGRLLAGMTAVATVSLSEKEGLIVPLSAVERDNGKAYVFTVVDGLARKRLVSLGA
                     SDGSEVQIISGIKSGELVATSNVSSLEDGSRVAQ"
     gene            complement(1303..1998)
                     /locus_tag="VFL04_04390"
     CDS             complement(1303..1998)
                     /locus_tag="VFL04_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7838972.1"
                     /translation="MPKKNVIIAVLAVALVGVALIVAYYAYEGYRYARTDDARVASNV
                     VGVIPEIPGKLIEWNVKEGDHVAKGAVLGRQDLGSALSSGAVSPQTMGAVAGVIAEKA
                     ALKAPLDGQVILSTAVVGQMAGPGMSLAVIADTDGLYISANIKEGQIARVRVGQNVDI
                     TVDAFPGKRFHGRVDSIGRATASTFSLLPSQGGGGNYTKVIQVIPIKVAILDAGDSRL
                     MIGMNATVRIHLN"
     gene            complement(2034..>2769)
                     /locus_tag="VFL04_04395"
     CDS             complement(2034..>2769)
                     /locus_tag="VFL04_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7838973.1"
                     /translation="VTTIGMYAGLFYIPLFLQSIRGIGAMETGLLMMPGALVTAVMMP
                     ITGKLFDKVGARALVIAGLFMLAFLTLNFANLNLATATSTITMWIVFRGMVMAFAMMP
                     AQTASLIDIPTDLISRASSISNIIRSVAGSFGLAILTSILTARQAFHGARLSWTLTLS
                     NPVVADTVSKIAGLAGGGTKGRGLAAAYLFGQVSKTSFVNAIDDVFIVAGMFTFVALV
                     PALFLKSKKAQTAAAGAHRTTAMAAE"
BASE COUNT          406 a          861 c         1013 g          489 t
ORIGIN      
        1 gccgcgccct gcacgcccgg gtcgttcctg aggcccgagg ccacggcctc gtccaccgag
       61 aggggcttgg cgccctgggc ccaggccgcg ccggtcccga agagccccgc ggccgcggca
      121 aggagggcag ccgccctcag cgttgcgcgg aacagcctca ttgggccacc cgcgagccgt
      181 cctcgagcga ggacacgttg gacgtggcca cgagctcgcc gctctttatg ccggagatga
      241 tctggacctc gctgccgtcg ctcgcgccga ggctcacgag gcgcttcctg gcgaggccgt
      301 cgacgaccgt gaacacgtag gccttgccgt tgtcgcgctc cacggcggag agcgggacga
      361 tgaggccctc cttctccgag aggctgaccg tggccacggc ggtcatgccg gcgaggagcc
      421 tgccgtcgtt ccgcaggccc accgagacgg ggaagtactg gcccgtggtc gccgcgatgg
      481 ggccgagctg ggagatggtc ccctcgtact cgcggtcggg catcccgtcg accctgatcc
      541 tgaccttgtc cccgaccgcg aggtggaaga cgtcgccctg gggcacggtg ccctggagct
      601 tgaggttgcc cgtgtcggct atcgtcatga tggcggtgga ggtcgaggtg agctcgccga
      661 ggttgaggtt ccggttcacg acggtgccgg agatggggct cgtgatcgtg gagttggaga
      721 tctggacccg tatgaggttg gcctgggcct cggcctgggc gaggcccgag ccgccgaccg
      781 tctggtactg gcggttcgcg ttgtcgaagg ccgccttggc gaggtcgagc ttggtctggg
      841 cgtcgtcggc cgcgctctgg gtcaccgact tcgagtcgag gagggccttg gtcctgtcgt
      901 agttcttctg ggccatgtcg aggttgagcc tcgcgctctc gatgcctacc ttggcctggg
      961 cggcctggtc gcgcaccgtc tggaccgagg cctcggccac ctggagctgg gcgaggagct
     1021 ccttcgcgtc gatctggacg aggacctggc cctcggccac cctgtcgccg gtgtcgacgg
     1081 ccacgctcct cgcgatgccg acgagcttgg ggaagatcgt caccgtcttg ttcgggacga
     1141 ggacgccgga catctcgacc cggtcggcta tcttgcccat cctcggggtc agggccttca
     1201 cggccacggg atcggcctgt ctgggcgcgc aggaggcgag gacgatgcct gcggtcagga
     1261 ccatgcaata gaatgctttg ttcaccatgt ttcggggact ccctagttca gatggatgcg
     1321 gactgtcgcg ttcatgccga tcatgaggcg cgaatcccct gcgtcgagga tcgccacctt
     1381 gatcgggatc acctggatca ccttcgtata gttgccgccc ccgccctggg agggcaggag
     1441 ggagaaggtc gaggcggtgg cccggccgat cgagtcgacc cggccgtgga agcgcttgcc
     1501 ggggaaggcg tcgacggtga tgtcgacgtt ctgcccgacg cgcacccggg ctatctggcc
     1561 ctccttgatg ttggcgctga tgtacaggcc gtcggtgtcg gctatcacgg cgagggacat
     1621 gccggggccg gccatctggc cgacgacggc cgtcgagagg atcacctggc cgtccagggg
     1681 ggccttgagc gcggccttct cggcgatgac cccggcgacg gcgcccatgg tctgggggct
     1741 gacggctccc gacgagaggg ccgacccgag gtcctggcgg ccaaggaccg cgcccttggc
     1801 gacgtggtcg ccctctttca cgttccactc gatgagcttc ccgggtatct cggggatgac
     1861 cccgacgacg tttgaggcca cgcgggcatc gtcggtcctc gcatagcggt agccctcgta
     1921 ggcgtagtag gcgacgatga gggcgacgcc gacgagggcc acggcgagca ctgcgatgat
     1981 cacgttcttc ttgggcatct atgcttctcc ctgttgctgt ttgggtacgc tggtcattcc
     2041 gccgccatcg cggtggtcct gtgggccccg gcggcagccg tctgggcctt cttgctcttc
     2101 aggaagagcg cgggcaccag ggcgacgaag gtgaacatgc ccgcgacgat gaacacgtcg
     2161 tcgatggcgt tcacgaagga ggtcttcgag acctggccga agaggtaggc ggcggcgagg
     2221 cccctcccct tcgtcccacc gccggcgagc cccgctatct tcgagaccgt gtcggcgacg
     2281 acggggttcg acagggtgag ggtccaggaa agccgggcgc cgtggaaggc ctggcgggcg
     2341 gtgaggatcg acgtgagtat ggcgagcccg aaggagcccg cgacgctcct tatgatgttg
     2401 gagatcgagc tggcccggct gatgaggtcg gtgggaatgt cgatgagcga ggccgtctgc
     2461 gcgggcatca tggcgaaggc catgaccatg ccgcggaaga cgatccacat cgtgatggtc
     2521 gaggtggccg tcgccaggtt gaggttggcg aagttcaggg tcaggaaggc gagcatgaag
     2581 aggccagcga tgacgagggc cctcgcgccc accttgtcga agagcttgcc ggtgatgggc
     2641 atcatgaccg cggtcacgag ggctcccggc atcatgagga ggccggtctc catggccccg
     2701 atgcccctga tgctctggag gaagaggggt atgtagaaca ggcccgcgta catgccgatg
     2761 gtcgtgacg
//
LOCUS       DASSQV010000257         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_310538, whole genome shotgun sequence.
ACCESSION   DASSQV010000257 DASSQV010000000
VERSION     DASSQV010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310538"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFL04_04400"
     CDS             complement(<1..641)
                     /locus_tag="VFL04_04400"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HET7838974.1"
                     /translation="MSAFEPSPRSLGSAASRSLVAEAALAPKPGLVDPRRPGPHADMD
                     FELLVASARALEPCFVGCAAEGRAAAQDGARPGGMLPTLRAIGLKGEEAMFRTTRGVN
                     THKGSVFALGLLVAASSYDLARGGGARGTRPPFGDRACARAAEILRGLGPELVIGNGR
                     ERSHGERLFDEYGIRGARGQAEDGFPVLRGSVLPVLRAAASAGKAMEVASVDAL"
     gene            806..1786
                     /locus_tag="VFL04_04405"
     CDS             806..1786
                     /locus_tag="VFL04_04405"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7838975.1"
                     /translation="MKKSVSVLVALVLAFGFVALASAAPAYPSKPLEIVAPANPGGGF
                     DALARAVDRALNVEKLYPQPTAVVNKPGAGGAVGFSYMQMKAKDSYELTVFSPPLIIG
                     NVNGTFPGKYTELTPLAKLITDYQIFLVRADSPYKTFKDLMDAVKKDPAAVKFAGGSA
                     AGNMDHLAFCKVAKAYGVDPSKLVYVAFAGGGEAIITLLGGNVAFVSTGVGEALGQIQ
                     AGTVRALAVTSPERRSGVLASVPTVKEAGIDVTYEVWRGLFGTQGMSQEAKDWWAKSL
                     KTMSQTATWKDSCDKLQWVNAYADSAAFAKFLTEEYNSYSALMKDLGLAK"
BASE COUNT          334 a          773 c          620 g          279 t
ORIGIN      
        1 agggcgtcga ccgaggcgac ctccatcgcc ttgcccgccg aggccgccgc ccgcagcacc
       61 ggcaggaccg agccgcgcag cacggggaag ccgtcctcgg cctggcccct ggccccgcgg
      121 atcccgtact cgtcgaagag ccgctctccg tggctcctct ccctgccgtt cccgatcacg
      181 agctccggcc cgagcccgcg gaggatctcg gctgcccgcg cgcaggccct gtcgccgaag
      241 ggcggacggg tccccctcgc gcccccaccc cgggccaggt cgtaggagga ggcggcgacg
      301 aggaggccga gggcgaagac cgagcccttg tgggtgttca ccccgcgggt tgtgcggaac
      361 atggcctcct cgcccttgag gccgatggcc ctcagggtag ggagcatccc gcccggcctg
      421 gccccgtcct gggcggcggc cctcccctcg gcggcgcagc cgacgaagca gggctcgagg
      481 gcgcgggccg aggccacgag gagctcgaag tccatgtcgg cgtgggggcc agggcgcctc
      541 gggtccacga ggccgggctt cggggcgagg gccgcctcgg cgacgaggga tcggctggcc
      601 gcagagccga gggaccgggg cgagggctcg aaggcgctca tcgccagaat atatcgcgcc
      661 cgggcggccc tcctccagat cccgaagccc cgtaggactg acacctacag cggaatgaag
      721 cctcctccta cataccggag ccgaacccgc gtctacactg ccctcaaaca agggagtccc
      781 tcccaatccc cccaaggagc acagcatgaa gaagagcgta tccgtcctcg tcgctctcgt
      841 cctcgccttc ggcttcgtgg ccctcgcctc ggccgcgccc gcctacccct ccaagcccct
      901 cgagatcgtt gcccccgcca acccgggcgg cggcttcgac gccctcgcgc gcgccgtcga
      961 ccgcgccctc aacgtcgaga agctctatcc ccagccgacc gcggtcgtga acaagcccgg
     1021 cgccggcgga gccgtgggct tctcctacat gcagatgaag gccaaggaca gctatgagct
     1081 gaccgtcttc tctccccccc tcatcatcgg aaacgtgaac gggaccttcc ccgggaagta
     1141 caccgagctc acccccctgg ccaagctgat caccgactac cagatcttcc tcgtcagggc
     1201 cgactcgccc tacaagacct tcaaggacct catggacgcg gtcaagaagg accccgccgc
     1261 cgtcaagttc gccggcggct cggccgcggg caacatggac cacctcgcct tctgcaaggt
     1321 cgcgaaggcc tacggcgtcg atccctcgaa gctcgtgtac gtcgccttcg ccggcggcgg
     1381 cgaggccatc atcaccctcc tcggcggcaa cgtggccttc gtctccaccg gcgtcggcga
     1441 ggccctcggc cagatccagg ccggcaccgt ccgcgccctc gcggtcacct cccccgagcg
     1501 ccgctcaggc gtcctcgcct cggtcccgac cgtcaaggaa gccggcatcg acgtgaccta
     1561 cgaggtctgg cgcggcctct tcggcaccca gggcatgagc caggaggcca aggactggtg
     1621 ggccaagtcc ctcaagacca tgtcccagac cgccacctgg aaggactctt gcgacaagct
     1681 ccagtgggtg aacgcctacg ccgactcggc cgccttcgcc aagttcctga ccgaggagta
     1741 caactcctat tcagccctca tgaaggacct cggcctggcg aagtaggccg ccgcgccaga
     1801 cccatcaaaa cctaccgggg ccccctcgac ccacgaagcg gccgagccgg gccccggccc
     1861 ccttccaacg gagacatcca tggcagctaa ggcaataaag cgcgaggagc gcatcgcgag
     1921 cgtgatcatg acgatcttcg ccgcgtccta cttcgcgggc accttcatct tcataccgaa
     1981 gtcctccata catcaggtgg tggggc
//
LOCUS       DASSQV010000258         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2425056, whole genome shotgun sequence.
ACCESSION   DASSQV010000258 DASSQV010000000
VERSION     DASSQV010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2425056"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1871)
                     /locus_tag="VFL04_04410"
     CDS             complement(<1..1871)
                     /locus_tag="VFL04_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7838976.1"
                     /translation="MPKSLLVDPAVVRKPGSLEIASIPLNRYVPDYKAELARFGAAGL
                     VALWRDMVCIREFETMLNSFKTMGSWNGIEYNHKGPAHLSIGQEAASVGQCASLNPDD
                     YIFGSHRSHGEILAKCLSASRILDEKRLQGIMEGFLGGETLKAVEGLKSSGVRDLAET
                     FTLFGTLAEIFARKAGFNRGLGGSMHAFFTPFGSMPNNASVGGSADIATGSALYTRIN
                     RKPGIVVANIGDASMGCGPVWEAMSLAAMDQYRSLWPKDVGGAPPLLFNFFNNFYGMG
                     GQTDGETMGYKVLARVGAGVNPENMHAERVDGYNPLAVAEAVARKKKILLEGRGPVLL
                     DTITYRISGHSPSDASSYRSKEEIELWQGADPIASYGDYLVQNKATTRAELEAIRAAV
                     LEKLHAVVRVATDDAACPRVDGAFIESVMFSNGRVENLDQAATPEGRPRKPELSQDLL
                     ENPRVKALAGKARYAFDAEGKAVPKNKLFQYRDALFEAMLHRFSVDPTMAAWGEENRD
                     WGGAFAVYRGLTEALPYHRLFNATISEGAIVGSGVGYALSGGRAVVELMYCDFMGRAG
                     DEIFNQASKWQSMSAGLLKMPLVLRVSVGNKYGAQHSQDWTALVAHVPGLKAMFPATP
                     "
     gene            complement(1881..>2280)
                     /locus_tag="VFL04_04415"
     CDS             complement(1881..>2280)
                     /locus_tag="VFL04_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7838977.1"
                     /translation="AEAGSEGRANIMRYNAVPWAVYGIPEAAGVGMTEQEALAKGIAV
                     KKAAMPLRVSGRFCAENGFAAQGAVKVIADAATDLILGVHVVGSYASEMIWGGAALIE
                     QELRVADAKEIIFPHPSVCEAIREAVWELE"
BASE COUNT          323 a          728 c          849 g          380 t
ORIGIN      
        1 ggggtggcgg ggaacatggc cttgagcccc ggcacgtggg ccacgagggc cgtccagtcc
       61 tgactgtgct gggccccgta cttgttgccc accgagacgc ggaggaccag gggcatcttg
      121 aggaggcccg ccgacatcga ctgccacttg gaggcctggt tgaagatctc gtccccggcc
      181 ctgcccatga agtcgcagta catgagctcg acgaccgcgc ggccccccga gagggcgtag
      241 cccacgcccg agccgacgat ggcgccctcg gagatcgtgg cgttgaagag cctgtggtag
      301 ggcagggcct cggtgaggcc gcggtagacg gcgaaggccc cgccccagtc gcggttctcc
      361 tcgccccagg ctgccatggt cgggtcgacc gagaagcggt ggagcatcgc ctcgaagagg
      421 gcgtcgcggt actggaagag cttgttcttg gggacagcct tgccctcggc gtcgaaggcg
      481 tagcgggcct tgccggcgag ggccttgacc ctcgggttct cgaggaggtc ctgggagagc
      541 tcgggcttgc gcgggcgccc ctcgggcgtc gcggcctggt cgaggttctc gaccctgccg
      601 ttggagaaca tcaccgactc gatgaaggcc ccgtcgaccc gggggcaggc cgcgtcgtcg
      661 gtggccaccc gcacgaccgc gtggagcttc tcgaggacgg cggcgcggat ggcctcgagc
      721 tcggccctgg tcgtcgcctt gttctgcacg aggtagtcgc cgtagctggc gatggggtcg
      781 gcgccctgcc agagctcgat ctcctccttc gagcggtagc tcgaggcgtc cgaggggctg
      841 tggccggaga tccggtaggt gatcgtgtcg aggaggacgg ggccgcggcc ctcgaggagg
      901 atcttcttct tgcgcgccac cgcctcggcg acggcgaggg ggttgtagcc gtcgacccgc
      961 tcggcgtgca tattctcggg gttgaccccc gcgcccaccc tggcgaggac cttgtagccc
     1021 atggtctcgc cgtcagtctg gccgcccatg ccgtagaagt tgttgaagaa gttgaagagg
     1081 aggggcgggg ccccgccgac gtccttgggc cagaggctcc ggtactggtc catcgcggcg
     1141 aggctcatcg cctcccagac ggggccgcag cccatggagg cgtcgccgat gttggcgacg
     1201 acgatgcccg gcttgcggtt gatccgcgtg tagagggccg agccggtcgc gatgtcggcc
     1261 gagccgccga cgctcgcgtt gttcggcatc gagccgaagg gggtgaagaa ggcgtgcatc
     1321 gagccgccga gcccgcggtt gaagccggcc ttgcgggcga agatctcggc gagggtgccg
     1381 aagagggtga aggtctcggc gaggtcgcgg accccggagc ttttgaggcc ctcgacggct
     1441 ttcagggtct cgccgccgag gaagccctcc atgatcccct ggagccgctt ctcgtcgagg
     1501 atccgcgagg ccgagaggca cttggcgagg atctcgccgt ggctccggtg cgagccgaag
     1561 atgtagtcgt cggggttgag gctggcgcac tggcccaccg acgcggcctc ctggcctatg
     1621 gagaggtggg ccgggccctt gtggttgtac tcgatcccgt tccaggagcc catggtcttg
     1681 aaggagttga gcatggtctc gaactccctg atgcagacca tgtcccgcca cagggccacg
     1741 aggccggcgg cgccgaagcg cgcgagctcg gccttatagt cggggacgta gcggttcagg
     1801 ggaatgctgg ctatctcgag gctgcccggc ttgcggacga ccgcggggtc gacgagaagt
     1861 gacttgggca tcgtgctctc ctattccagt tcccacaccg cttcgcggat ggcctcgcag
     1921 accgagggat gggggaagat gatctccttc gcgtcggcga cgcgaagctc ctgctcgatg
     1981 agggcggccc cgccccagat catctccgag gcgtaggagc cgacgacgtg gacgccgagg
     2041 atgaggtcgg tcgccgcgtc ggcgatgacc ttgaccgcgc cctgggccgc gaagccgttc
     2101 tcggcgcaga agcggcccga gacgcgcagg ggcatggcgg ccttcttcac ggcgatgccc
     2161 ttggccaggg cctcctgctc ggtcatgccg accccggcgg cctcggggat gccgtagacg
     2221 gcccagggga cggcgttgta gcgcatgatg tttgccctgc cctcgctccc ggcctcggcg
//
LOCUS       DASSQV010000259         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_61944, whole genome shotgun sequence.
ACCESSION   DASSQV010000259 DASSQV010000000
VERSION     DASSQV010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61944"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VFL04_04420"
     CDS             <1..926
                     /locus_tag="VFL04_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7838978.1"
                     /translation="DRDRLHDRIRRLLFASPALARAFARGDFRSVFRGRGIEFDSLRD
                     YSPDDDARLIDFRSTLRLGRPFVRTYREDRSLTVFLLVDVSASMDMGSGELSKLDMAV
                     LASSLVAYAAQFRGLPVGCLVFADRIVGRLAPRRGKAHALAVAEAAVLAAAREGRGAL
                     RGDEAALRDQAEVRGSGLAQAVRSASLILKRRSLVMVLSDFRGGDWARPLGELARRHD
                     VVAVRLSDALDLELPRRGSFGVVDPETGQGAWLPLGSRGFRGRWRERGLAWRAEAERG
                     LSDARVPLLELDTSDDPALRLLQFFERRKGS"
     gene            923..1837
                     /locus_tag="VFL04_04425"
     CDS             923..1837
                     /locus_tag="VFL04_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838979.1"
                     /translation="MRPIHRAAVLLCALALGAVSANAAPLRLDHLSFLPPVFGAGEEV
                     EASAKLPGADAAGVSAISYRPATAAQAGSGDPELREIRVYRTPSGWELRIRFVPWSPG
                     PGSLPAWRSKGVLIPPVPYVAASALGAAMGNPRGLELSPPRPQAEPPGTAVYVYGFAA
                     FVLLLVLVCLGLVAWLIPAARTLLAKRRAAQAYRRLCRDMDFLATQAEGGDPVPFYSA
                     LARALRIYLGERVEAGVPMLSARELGLLPQEAFPAEGIREAAASLLAETEEVRYAGLL
                     PAAGEMRQATERARAIGAKAEEALDAGL"
     gene            1824..2813
                     /locus_tag="VFL04_04430"
     CDS             1824..2813
                     /locus_tag="VFL04_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7838980.1"
                     /translation="MLAFERPWALALLLLVPALMSLNLLLRGRKAILAFPFDLGSPLR
                     LPLLPRVLRLLRETAVWLGLAACVVAAAGPALVTRKLSYLGRGNEVVFVIDVSPSMAA
                     SDLAPTRLDAAKAIITGFLQARRNETVGLVAFGQEAALICPPTQDYASFASRLGGLAP
                     GLFGDGTALGDGIATAIAHAARSSAPEKHIILLTDGESNAGSLDPATAAAIAARNGIE
                     LSVVGVGSKGEVPVSYLDPATGKKRTGIYRSGFEREALERIALAGGGRYYAAENAEGL
                     ASAFSEISERSVSLTRSRSSNVEEPLGGRLLLLALAALAIARVLDILGGGGRP"
BASE COUNT          327 a         1157 c          952 g          413 t
ORIGIN      
        1 tggacaggga ccggctccac gaccggatcc ggcgcctcct cttcgcgtcg ccggctctcg
       61 cgcgggcctt cgcccgcggg gacttccgct cggtcttccg gggacggggc atcgagttcg
      121 actcgctcag ggactactcg cccgacgacg acgcgaggct catcgacttc cgctcgaccc
      181 tccgcctcgg gcggcccttc gtgcgcacct accgcgagga ccgctccctc accgtcttcc
      241 tcctcgtcga cgtctccgcc tccatggaca tgggctcggg ggagctctca aagctcgaca
      301 tggccgtcct cgcctcgtcg ctcgtggcct acgcggccca gttccgcggc ctccccgtgg
      361 gctgcctcgt cttcgccgac aggatagtcg gccgtctggc cccgaggcgg ggcaaggccc
      421 acgccctcgc ggtggccgag gccgccgtcc tggccgccgc ccgcgagggc cgcggcgccc
      481 tccgcggcga cgaggccgcg ttgcgcgacc aggccgaggt ccgcggcagc ggcctggccc
      541 aggcggtcag gagcgcctcg ctcatcctca agcgccgcag cctggtcatg gtcctgagcg
      601 atttcagggg cggggactgg gccaggcccc tcggcgagct ggcccgccgc cacgacgtgg
      661 tcgcggtgag gctctccgac gccctcgacc tcgagctccc gcgccgcggc tcctttggcg
      721 tcgtcgaccc cgagaccggc cagggggcct ggctccccct gggcagccgc ggcttccgcg
      781 ggcgctggag ggagcgcggc ttggcctggc gggcggaggc cgagcgcgga ctctcggacg
      841 caagggtccc cctcctcgag ctcgacacct cggacgaccc cgccctccgc ctcctccagt
      901 tcttcgagcg gaggaaggga tcgtgaggcc gattcaccgc gccgccgtcc tcctctgcgc
      961 cctcgccctc ggcgcggttt cggccaacgc ggcccccctc aggctcgacc acctgtcctt
     1021 cctgcccccg gtcttcggcg ccggcgagga ggtcgaggcc tcggccaagc tccccggggc
     1081 cgacgccgcc ggggtgtcgg ccatctccta ccgtccggca accgcggccc aggcggggag
     1141 cggtgacccc gagctccgcg agatcagggt ctaccggact ccctcgggct gggagctcag
     1201 gatcaggttc gtgccctggt cgccggggcc cggcagcctg cccgcgtggc gctcgaaggg
     1261 cgtcctgatc ccgcccgtac cctatgtcgc cgcctcggcc ctcggcgccg cgatgggcaa
     1321 cccgcggggc ctcgagctct ccccgcccag gccccaggcc gagcccccgg ggacggctgt
     1381 ctacgtctac ggcttcgccg ccttcgtcct cctcctcgtc ctggtctgtc tgggcctcgt
     1441 ggcctggctc atcccggccg ccaggaccct gctcgccaag cgccgcgccg cccaggccta
     1501 ccgcaggctc tgccgcgaca tggacttcct cgccacacag gccgagggcg gggaccccgt
     1561 gcccttctat tcggccctcg cgagggccct gcgcatctac ctcggggaga gggtcgaggc
     1621 cggggtcccg atgctgagcg cgagggagct cggcctcctt ccccaggagg ccttccccgc
     1681 cgagggcata agggaggccg ccgcctcact cctcgccgag accgaggagg tgcgctacgc
     1741 cggcctcctg cccgcggccg gggagatgag gcaggccacc gaacgcgccc gcgcgatcgg
     1801 cgccaaggca gaggaggccc tcgatgctgg cctttgagcg gccctgggcc ctcgccctcc
     1861 tcctcctcgt gcccgccctc atgtcgctca acctcctcct caggggaagg aaggccatcc
     1921 tggccttccc cttcgacctc gggagcccgc tccgcctgcc cctcctcccg agggtcctgc
     1981 gcctcctccg cgagacggcg gtctggctcg ggctcgccgc ctgcgtggtg gccgccgccg
     2041 gcccagccct cgtgacgcgg aagctctcct acctgggcag gggcaacgag gtggtcttcg
     2101 tcatcgacgt ctcgccgagc atggccgcct ccgacctcgc gcccaccagg ctcgacgcgg
     2161 ccaaggccat catcacgggc ttcctccagg cgaggcgcaa cgagaccgtg ggcctcgtcg
     2221 ccttcggaca ggaggccgcc ctcatctgcc cgccgaccca ggactacgcc tccttcgcct
     2281 caaggctcgg gggcctcgcg cccgggcttt tcggggacgg cacggccctc ggcgacggga
     2341 tcgcgacagc catcgcccac gctgccaggt ccagcgcccc ggagaagcac atcatcctcc
     2401 tcaccgacgg cgagagcaac gcgggctccc tcgacccggc cacggcggcg gcgatcgccg
     2461 cgcgcaacgg catcgagctc tcggtcgtcg gggtgggcag caagggcgag gtccccgtct
     2521 cctacctcga ccccgcgacg ggcaagaaga ggacggggat ctaccgcagc ggcttcgagc
     2581 gcgaggccct ggagaggatc gccctcgcgg gcgggggcag gtactacgct gctgagaacg
     2641 ccgagggcct cgcctcggcc ttctcggaga tctcggagcg ctcggtctcc ctgacccgct
     2701 cgcggagcag caacgtcgag gagcccctgg gcggccggct cctcctcctg gccctcgccg
     2761 ccctcgccat cgcccgggtc ctcgacatcc tcggcggcgg aggccggcca tgagcctcgc
     2821 ccatcccgag ctcctcgccc tggccctcc
//
LOCUS       DASSQV010000260         3687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_62084, whole genome shotgun sequence.
ACCESSION   DASSQV010000260 DASSQV010000000
VERSION     DASSQV010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62084"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFL04_04435"
     CDS             <1..197
                     /locus_tag="VFL04_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET7838981.1"
                     /translation="RARLHSIPGSPPSLVDMPDACPFNPRCPHAMDICRRSYPPETAL
                     QGRLGARTVNCWLHAGEGER"
     gene            194..1198
                     /locus_tag="VFL04_04440"
     CDS             194..1198
                     /locus_tag="VFL04_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET7838982.1"
                     /translation="MSETETLLEVRGLKVHFPIEKGIAFRRRVGWVRAVDGVDFRLAR
                     GEALGLVGESGCGKSTTAMAVAQLQGLSSGQILFEGRDLAGLDARELRRARMNFQVVF
                     QDPYSSLNPRMRTLDIIAEPLRIYARSGIIDLGKAEIEAKTLELMERCGLPGSFARRY
                     PHEFSGGQRQRIGIARALALGPKLVIADEPVSALDVSIQSQILNLLKDLQADFDLTFL
                     FIAHDLGVIEYFCSRIAVMYLGRIVETADSEELYRSPMHPYTRALLSAVPLPDPEKER
                     GRRRIILKGDVPSPSVEHRGCPFMERCPEAMPRCAERPPRLAPVEEGHEVSCFLYPGS
                     "
     gene            1210..2409
                     /locus_tag="VFL04_04445"
     CDS             1210..2409
                     /locus_tag="VFL04_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET7838983.1"
                     /translation="MEIKVFRLRLPLRNPFRIAHGSYAWRENIFHWIGHGGGFGMGEA
                     PVVPYYGISADEIEAELRAAVSPRLVEAALEGGRIVGAALEGGRAYGAALEDGRADGA
                     AFAHPVSRSAIETAVLALRARLTGLKAAELLGFSPAELGCGTRAPASSFTVAYDEDAE
                     AMARIAAESGFRRLKIKAGIPGDVERIRLLRERLPEAIIRVDANQGWSLEEAPARIAE
                     LEALGVELIEEPIRGGPRELERLAAGTGVPILLDESARDLEQVRLYAKEAPSVAGIVI
                     KTAKNGGPSASLELARAARDEGMEVMVSSMVETSLGLSSALPLAPLCRWCDLDAPLLL
                     GSDPFTGLSYEAEAPVLAPEGVLPGPELAALIAGLPSLARSNVRGGAGGEARGGTWGG
                     PGGGAAL"
     gene            2421..2927
                     /locus_tag="VFL04_04450"
     CDS             2421..2927
                     /locus_tag="VFL04_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7838984.1"
                     /translation="MVSADAAACSAIVCSSEIGRRYGFRLEGMEATLLAALAAEGGLF
                     VAEGWGAGEENLGGQSPDQAGAPALLGFAWIDPHGAFSTAPYLRLIAVDEAARGRQVG
                     SALLAEFEARTARIGRDWCLLVSDFNLPAQAFYGRHGYAKAGALPDFARPGITEILMV
                     KRRGRITP"
     gene            2924..>3687
                     /locus_tag="VFL04_04455"
     CDS             2924..>3687
                     /locus_tag="VFL04_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7838985.1"
                     /translation="MSPEPGIPALDEIAQRWLEAGFFAGAVLRVERGGALLHEAAWGQ
                     SLRIPEQSSRLEPGCLFDLASVTKLFTTTAVLRLVSLGGLRLHDPVAELLCPGLDPSL
                     GSRLRASLGGADVASLLAHSSGIHYWYPFYTRRGEAFEAILADVLKAHPRRAEVVYSD
                     LNFMILGRILELRTGLPLRSAMDDLLLRPLGLRRTSYAPPKGPSAAGEFGNRIERRMV
                     ADLGLHFPSWRDESRPIQGEADDGNCFYYFGGEAGHA"
BASE COUNT          531 a         1330 c         1264 g          562 t
ORIGIN      
        1 agagggccag gctccactcg atcccgggct cgccgccgag cctcgtcgac atgcccgacg
       61 cctgcccctt caacccgcgc tgcccccacg cgatggacat ctgccgccgc tcctatccgc
      121 ccgagacggc gctccagggc aggctcgggg cgaggaccgt gaactgctgg ctccacgccg
      181 gggagggaga gcgatgagcg aaaccgagac cctcctcgag gtgcggggcc tcaaggtcca
      241 cttcccgatc gagaagggca tcgccttcag gaggcgggtc ggatgggtgc gcgcggtcga
      301 cggggtcgac ttccgcctcg ccaggggcga ggccctcggc ctcgtgggcg agtcgggctg
      361 cggcaagtcg acgacggcca tggccgtcgc ccagctccag ggcctgagct ccggccagat
      421 cctcttcgag ggccgggacc tcgccggcct cgacgcccgc gagctcaggc gcgcgaggat
      481 gaacttccag gtcgtgttcc aggaccccta ctcctcgctc aacccgcgga tgcgcaccct
      541 cgacatcatc gccgagcccc tgcggatcta cgcgcggagc ggcatcatcg accttggcaa
      601 ggccgagatc gaggccaaga ccctcgagct catggagcgc tgcggccttc cgggttcctt
      661 cgcaaggcgc tacccccacg agttctccgg cggccagagg cagcgcatcg gcatcgccag
      721 ggccctcgcc ctggggccca agctcgtgat cgccgacgag cccgtgtcgg ccctcgacgt
      781 ctcgatccag tcccagatcc tcaacctcct caaggacctc caggccgact tcgaccttac
      841 cttcctcttc atcgcccacg acctcggggt catcgagtac ttctgctcgc ggatcgccgt
      901 gatgtacctc ggccgcatcg tcgagaccgc ggactcggag gagctctacc gctcgcccat
      961 gcacccctac acgagggccc tgctctcggc cgtgcccctc cccgaccccg agaaggagag
     1021 ggggaggagg cggatcatcc tcaagggcga cgtgccctcg ccgagcgtcg agcaccgggg
     1081 ctgccccttc atggagcgct gccccgaggc catgccccgc tgcgccgaga ggccgccgag
     1141 gctcgccccc gtcgaggagg ggcacgaggt ctcctgcttc ctctacccgg gctcctgagg
     1201 ggcggcggca tggagatcaa ggtcttccgc ctgaggctgc ccctgcgcaa ccccttccgg
     1261 atcgcccacg ggagctacgc ctggcgcgag aacatcttcc actggatcgg ccacgggggc
     1321 ggcttcggca tgggcgaggc tcccgtggtc ccctactacg ggatcagcgc cgacgagatc
     1381 gaggccgagc tgcgggcagc cgtatcgccg cggcttgtcg aggcggcctt ggagggcggc
     1441 cgcatagtcg gggcggccct ggagggcggc cgagcttacg gggcggcctt ggaggacggc
     1501 cgagcagatg gggcggcctt cgcccaccct gtgagccgct cggccatcga gacggccgtc
     1561 ctcgccctgc gcgccagact cactggcctc aaggccgccg agctcctcgg cttcagcccg
     1621 gccgaactcg gctgtgggac cagggcccca gcctcctcct tcacggtggc ctacgacgag
     1681 gacgccgagg cgatggcgcg catcgcggcc gaatccggct tccgccgcct caagatcaag
     1741 gccgggatcc ccggcgacgt cgagcggata aggctcctca gggagaggct gcccgaggcc
     1801 atcatcaggg tggacgcgaa ccagggctgg agccttgagg aggcgcccgc gaggatcgcc
     1861 gagctcgagg ccctcggagt cgagctgatc gaggagccga tcaggggcgg cccccgcgag
     1921 ctcgagcggc tcgccgcggg gacaggcgtg cccatcctcc tcgacgagag cgcccgcgac
     1981 ctggagcagg taaggctcta cgctaaggag gccccctcgg tggcgggcat cgtcatcaag
     2041 accgcgaaga acggcgggcc ctcggcgagc ctcgagctgg ccagggccgc ccgcgatgag
     2101 ggcatggagg tcatggtgag ctctatggtc gagacgagcc ttggcctaag ctcggccctt
     2161 cccctcgccc ccctctgcag atggtgcgac ctcgacgccc ccctcctcct cggctccgat
     2221 cccttcaccg gcctctccta cgaggccgag gccccggtgc tggcccccga gggcgtcctg
     2281 cccggaccgg agctggctgc cctgatcgcg ggtctgccga gcctcgcgcg aagtaatgtt
     2341 cggggcgggg ccggtggcga ggcccgtggc gggacttggg gcgggccagg gggcggggca
     2401 gcactatgag gatcagggac atggtaagcg ccgacgccgc ggcctgctcg gccatcgtct
     2461 gctcgtcgga gatcggccgg cgctacggtt tcaggctgga agggatggag gcgaccctgc
     2521 tcgccgccct tgcggccgag ggaggcctgt tcgtcgccga gggctggggg gccggcgagg
     2581 agaatctcgg gggacagtca cccgaccagg ccggcgcccc ggcgctgctc ggattcgcct
     2641 ggatcgaccc ccatggcgcc ttctccacag ccccctacct tcgcctcatc gcggtggacg
     2701 aggcggcgcg cggcaggcag gtgggttcgg cgctcctcgc cgagttcgag gcgaggacgg
     2761 ctaggatcgg gagggactgg tgcctcctcg tgtcggactt taacctccca gcccaggcct
     2821 tttacgggcg ccacggctac gcgaaggccg gggccctacc cgatttcgcg agaccgggca
     2881 tcacggagat cctgatggtg aaacggcgag gccggattac gccatgagtc ctgaaccggg
     2941 gatccctgcc ctggacgaga tcgcccagcg ctggctcgag gcaggcttct tcgcgggtgc
     3001 cgtcctccgc gtcgagcggg gcggcgccct cctccacgag gcggcttggg gacagtcact
     3061 cagaattcct gagcaaagca gtcgccttga gccaggatgc cttttcgacc tcgcctcggt
     3121 caccaagctt tttacgacga cagccgtgct gcggctcgtc agcctcggcg ggctgcggct
     3181 ccatgacccg gtggctgagc ttctttgccc cggtctcgac ccgagcttgg gctcgcggct
     3241 ccgggcttcg ctgggcggag ccgacgtcgc ctccctcctc gcccattcct cgggcatcca
     3301 ctactggtac cccttctaca cgcggcgggg cgaggccttc gaggcgatcc tggccgacgt
     3361 gctcaaggcc catccccggc gggccgaggt ggtctactcc gacctcaatt tcatgatcct
     3421 tgggaggatc ctcgagcttc ggacgggcct gcccctgagg tccgcgatgg acgacctgct
     3481 gctccgcccc ctcgggctca ggcggacttc ctatgccccg cccaagggcc ccagcgccgc
     3541 tggcgagttc ggcaaccgca tcgagcgcag gatggtcgcc gatttgggcc tgcacttccc
     3601 ctcctggcgc gacgagtcaa ggccaatcca gggcgaggcc gacgacggga actgcttcta
     3661 ctacttcggc ggggaggcgg gccacgc
//
LOCUS       DASSQV010000261         3948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_311417, whole genome shotgun sequence.
ACCESSION   DASSQV010000261 DASSQV010000000
VERSION     DASSQV010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311417"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFL04_04460"
     CDS             complement(<1..386)
                     /locus_tag="VFL04_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7838986.1"
                     /translation="MNKSRSAPLYGIAVAVLWGLSFLSIKVAVVVVPPMTMAVARFII
                     ACAVLPLFALLLGERLGVAPRDLPVLALGGLFGVTIYFYCENNAVALLTASESSIIVG
                     TIPVVTMLAERLFVGTRLGLHSYAGAL"
     gene            complement(513..1730)
                     /locus_tag="VFL04_04465"
     CDS             complement(513..1730)
                     /locus_tag="VFL04_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HET7838987.1"
                     /translation="MNQFARKAALVLAIGLFLVIGTGFAAPAKFHIGVCTETVAQAED
                     DLRGAEQLIKEYGAVKDGGMIQNITYPDDFMSQQETVISNLVALADDPLMKAVVTCNA
                     LPGVSEAYRRIRAKRPDILLLAALPAEDPLVIQKAADLAIDADFVSRGYTIVWGAKQL
                     GAKTFVHISFPRHMSYESLGRRRAIMEEACKDLGLKFAFETAPDPTSDVGVAGAQQFI
                     LEKVPQWIQKYGPNGEKVAFFCTNDAHTEPLIKQILASKNGIFVEPDSASPLMGFPGA
                     LGLDLTKEAGNFPAIMKKVEAAVVAKGGAGRLGTWAYSTGFSMTAGLGELAKRVIEGK
                     AKVTNTKDIYDALGKYTPGAKWNGALYTDMNTGVKAKNDFLVYMDTYVFGKGYLGTPS
                     LKIPEKYYTLGKK"
     gene            1892..2473
                     /locus_tag="VFL04_04470"
     CDS             1892..2473
                     /locus_tag="VFL04_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838988.1"
                     /translation="MSRSELEAALDFILNKASDAEFDVITKACQRRIRDKTAFASLGG
                     EGPGAMARRMAEDLSQGVGATMESVRSTMKGFVSDIIRKNAPEISEEEIAALLDEYVP
                     PEGAQQPDAARSEASPFPPEALLGMVSSFIEYSEERMAPSRQKQLWDANPRWQDEYWR
                     AFPAEVKSLVKAYLEGRIDGDTFGTAILSVLGL"
     gene            2576..>3948
                     /gene="hutH"
                     /locus_tag="VFL04_04475"
     CDS             2576..>3948
                     /gene="hutH"
                     /locus_tag="VFL04_04475"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET7838989.1"
                     /translation="METVLIDGHSLSVKQLALAARYGSKVELKAEAAARIDESRRVVD
                     HCVDEGLVRYGITTGFGKFCDVVISKEENATLQRNLIMSHSCGVGDPLPGEVVRAMIL
                     LRANALAVGYSGIRLQTVKALLGMLNAGVSPVVPEKGSLGSSGDLVPLSHMVLVLLGM
                     GEAEYRGRRMSGAEALAAAGLHPVELAAKEGLALINGTQAMTAVGALACFDAKKLLRT
                     AELCAALTTQALHGITDAFDPRLHEIRGQIGQIGSAKALRDFLAGSRMTTRQGQERVQ
                     DAYALRCVPQVHGASRDAIEYCSGIVSRELNAVTDNPIIFPDGDIISGGNFHGQSVAL
                     AMDFLGIALAELANISERRIERLVNPSLSNGLPAFLSSNGGLNSGFMIMQYTAASLVS
                     ENKVLAHPASVDSIPSSANQEDHVSMGTIAARKARSILDNLRRVLAIEVLSACQAVDL
                     RGGPEGLS"
BASE COUNT          652 a         1311 c         1352 g          633 t
ORIGIN      
        1 agggccccgg cgtagctgtg cagcccgagc ctggtcccca cgaagaggcg ctcggccagc
       61 atggtgacga cgggtatggt gccgacgatg atcgaggact ccgaggccgt gagcagggct
      121 acggcgttgt tctcgcagta gaagtagatc gtcacgccga acagcccccc cagggccagg
      181 acgggcaggt cgcgcggggc gacaccgagc ctttcgccga ggaggagggc gaagagtggc
      241 aggacggcgc aggctatgat gaagcgggcg acggccatgg tcatcggggg cacgacgacg
      301 acggcgacct tgatgctgag gaaactcaag ccccagagca cggcgacggc aatgccatag
      361 aggggcgcgg agcggctctt attcatgcca tggatcgtag cctcgccggt cggcggattt
      421 ctagcggtgg ggggagccgg cccgccccga agggagggct ccggcgacag ggggcgcggc
      481 cccccttgat gggaggccgc gcgggcaaag cgctatttct tcccgagggt gtagtacttc
      541 tcggggatct tgagcgaggg cgtgccgagg tagcccttgc cgaagacgta ggtgtccatg
      601 tagacgagga agtcgttctt cgccttgacg ccggtgttca tgtcggtgta gagggcgccg
      661 ttccacttcg ctccgggggt gtacttgccc aaggcgtcat agatgtcctt ggtgttcgtg
      721 accttggcct tgccctcgat cacccgcttg gcgagctcgc cgagtccggc cgtcatggag
      781 aagcccgtgc tgtaggccca ggtgccgagc cttccggccc cgcccttggc gacgacggcg
      841 gcctcgacct tcttcatgat ggcggggaag ttgcccgctt ccttggtcag gtcaagaccg
      901 agggccccgg ggaagcccat gaggggggag gccgagtcgg gctcgacgaa gatgccgttc
      961 ttggaggcga ggatctgctt gatgaggggc tcggtgtggg cgtcgttcgt gcagaagaag
     1021 gcgaccttct cgccgttcgg gccgtacttc tggatccact gggggacctt ctcgaggatg
     1081 aactgctggg ctccggcgac gccgacgtcg gaggtcgggt cgggggcggt ctcgaaggcg
     1141 aacttgaggc cgaggtcctt gcaggcctcc tccatgatcg ccctgcggcg gccgaggctc
     1201 tcgtaggaca tgtggcgggg gaaggagatg tggacgaagg tcttggcgcc gagctgcttg
     1261 gctccccaga cgatcgtgta gccgcgggac acgaagtcgg cgtcgatggc gaggtcggcg
     1321 gccttctgga tcacgagggg gtcctcggcg ggaagggcgg cgaggaggag gatgtcgggg
     1381 cgcttcgccc tgatccgcct gtaggcctcg gagacgccgg gcagggcgtt gcaggtgacg
     1441 acggccttca tgagggggtc gtcggcgagg gccacgaggt tggagatgac ggtctcctgc
     1501 tgggacatga agtcgtcggg ataggtgatg ttctggatca tcccgccgtc cttgacggcc
     1561 ccgtactcct tgatcagctg ctcggcgccg cggaggtcgt cctcggcctg ggcgacggtc
     1621 tcggtgcaga cgccgatgtg gaacttggcc ggggcggcga agcccgtacc gatcacgagg
     1681 aagaggccga tcgccaggac gagggcggcc tttcgcgcga actggttcat gattcctcct
     1741 taaggaaacc aggcggaaaa tatctgccac aatacactgg cccatctcgg tgtcaagcca
     1801 aaacgtttgc gtacacggcg ccccgcgcgg ccgccctgcg cggccgaggc tcccacttta
     1861 cagggggccg cggccccgga atactgtccc catgtcgcgt tccgagctcg aggccgccct
     1921 cgatttcatc ctcaacaagg cgagcgacgc cgagttcgac gtcatcacca aggcctgcca
     1981 gcgcaggatc agggacaaga cggccttcgc gagcctcggg ggcgaggggc ccggggcgat
     2041 ggctcggcgc atggccgagg acctgagcca gggtgtcggg gcgacgatgg agagcgtccg
     2101 ctcgacgatg aagggcttcg tctccgacat catcaggaag aacgcccccg agatcagcga
     2161 ggaggagatc gccgccctcc tcgacgaata cgtcccgccc gagggcgccc agcagccgga
     2221 cgccgcgagg agcgaggcct cgcccttccc acccgaggcc ctcctcggca tggtcagctc
     2281 cttcatcgag tactccgagg agcgcatggc cccgagccgg cagaaacagc tctgggacgc
     2341 caacccgcgc tggcaggacg agtactggag ggccttcccc gccgaggtga agagcctcgt
     2401 gaaggcctat ctcgagggca ggatcgacgg ggacaccttc gggacggcca tcctctcggt
     2461 gctcgggctc tgaccacggc ggggccgttg ggccataagc gcccttccgg gtcccgggat
     2521 ttcatgatat gatcctgtcg tatcaattct cgcttccccc cagcaaggcg gtactatgga
     2581 aactgtcctc atcgatgggc atagcctgag cgtcaagcag cttgccctcg ccgcgcgata
     2641 cggctcaaag gtcgagctca aggccgaggc ggccgcaagg atcgacgaat cccgccgggt
     2701 ggtggaccac tgcgtcgacg agggcctcgt ccgctacggc atcacgaccg gcttcggcaa
     2761 gttctgcgac gtcgtcatct cgaaggagga gaacgccacc ctccagcgga acctcatcat
     2821 gagccactcc tgcggggtcg gcgatcccct ccccggggag gtcgtcaggg ccatgatcct
     2881 cctgcgggcc aacgccctcg ccgtcggcta ctccggcatc aggctccaga cggtgaaggc
     2941 cctcctcggc atgctcaatg cgggcgtgag ccccgtcgtg cccgagaagg gctcgctcgg
     3001 ctcatcgggc gacctcgtgc ccctctccca catggtcctc gtcctcctcg gcatgggcga
     3061 ggccgagtac cgcggcagga ggatgagcgg ggccgaggcc ctcgccgccg ccggcctcca
     3121 tccggtcgag ctcgcggcga aggagggcct ggccctcatc aacggcaccc aggccatgac
     3181 tgccgtgggc gccctcgcct gcttcgacgc gaagaagctc ctgcgcaccg ccgagctctg
     3241 cgccgccctc acgacccagg ccctccacgg gatcaccgac gccttcgacc cgaggctcca
     3301 cgagatccgc ggccagatcg ggcagatcgg ctcggccaag gccctccgcg atttcctcgc
     3361 aggctcgagg atgaccaccc gccagggcca ggagagggtc caggacgcct acgccctccg
     3421 ctgcgtgccc caggtccacg gggccagccg cgacgcgatc gagtactgtt cgggcatcgt
     3481 gtcgcgcgag ctcaacgcgg tcaccgacaa ccccatcatc ttccccgacg gcgacatcat
     3541 ctccggcggc aacttccacg gccagagcgt ggccctcgct atggacttcc tcggcatcgc
     3601 cctcgccgag ctcgcgaaca tctccgagcg ccgcatcgag aggctcgtga acccgagcct
     3661 ctccaacggc ctgcccgcct tcctctcctc caacgggggc ctcaactcgg gcttcatgat
     3721 catgcagtac accgccgcct ccctcgtgag cgagaacaag gtccttgccc acccggcgag
     3781 cgtggactcg atcccctcga gcgcgaacca ggaggaccac gtctccatgg gcacgatagc
     3841 cgcgcgcaag gcccgctcga tcctcgacaa cctcaggagg gtcctcgcga tcgaggtcct
     3901 ctcggcctgc caggccgtcg acctccgcgg cgggcccgag ggcctctc
//
LOCUS       DASSQV010000262         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1927156, whole genome shotgun sequence.
ACCESSION   DASSQV010000262 DASSQV010000000
VERSION     DASSQV010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927156"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1285)
                     /locus_tag="VFL04_04480"
     CDS             complement(29..1285)
                     /locus_tag="VFL04_04480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7838990.1"
                     /translation="MRDTEYRIYGYRWVVLGAFMLVNFIIQVLWISYAPITADAARFF
                     GVSELKIGFLAMVFMIVYVPLSIPASWAIDRYGFKKAAGLASIMMVVFGALRGFAGTS
                     YALALTGSLGLAVAQPLFLNAWTKLPAHWFPRGERATAVGLITLSNLLGIAGGMVATP
                     MLAPIVGIPGTQYIFSGFAALSTLAFLLAARESPPSPPGPVEADERALMFDGVKHALR
                     VPSFRLFLGVVFVGMGIFNGVTTWIEEIVRPRGFGPQEAGTFGALLLVGGILGAVAIP
                     ALSDRSGKRRPYMMLGIIATIPGLLGMTWFASFWPIAASAFVLGFFLTSVMPVGMQFS
                     TEITVPTPEGTSNGLIQLFGQASVVFVFIMDALRSRSGSLGPGMLLSCALLVVAAFLI
                     LRMSETGKAEEEAPRPTRAEPAADEG"
     gene            complement(1443..2348)
                     /locus_tag="VFL04_04485"
     CDS             complement(1443..2348)
                     /locus_tag="VFL04_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838991.1"
                     /translation="MRRTALVVVMTILASALALQAQSLIDNSFYRRSLDLSAQAQKAF
                     DAGDYDAAASLAAQAKENALLSDRYVEKMLALTAANKSIGLAKAQLAWADGRGAQASF
                     PELYAKAKDGMDAATKAYAAEDYAAAKDQADAVVAAVNAIAVADADAAIAAAKERLAW
                     AEGVGAQKRYPEDYSRASSSMDKALAAYGAQDFREAAIRARDVLDALTLVEASSPLPA
                     SYRVRLIPSLRDCLWRIAGYPFIYNDPYQWTVLYEANKKILRDPSDPNLILPGQVLRI
                     PSIGGEVRQGAYDPSANYEAFAKKK"
     gene            complement(2359..>2677)
                     /locus_tag="VFL04_04490"
     CDS             complement(2359..>2677)
                     /locus_tag="VFL04_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838992.1"
                     /translation="GAKEKSDQSKARSETIKAQVAVKDLYDAALAGYEEGISKLTSGD
                     YEGATAALQKSGTDFDAAYDAAAAKRAAAEAAMKAAQEAAGESEKKAAQADPIVNQSQ
                     AAQ"
BASE COUNT          404 a          869 c          970 g          434 t
ORIGIN      
        1 gcgggctcgg ccttcgcggg cggcgggctc agccctcgtc ggcggcgggc tcagccctcg
       61 tcggcctggg cgcctcctcc tcggccttgc ccgtctcgct catcctgagg atgaggaagg
      121 cggcgacgac gaggagggcg caggagagga gcatccccgg cccgagcgag cccgagcggc
      181 tgcgcagggc gtccatgatg aagacgaaga ccaccgaggc ctggccgaag agctggatca
      241 gcccgttaga ggtgccctcg ggggtgggga cggtgatctc ggtgctgaac tgcatgccca
      301 cgggcatgac gctcgtgagg aagaagccga ggacgaaggc cgaggcggcg atcggccaga
      361 acgaggcgaa ccaggtcatg ccgaggagcc ccggtattgt cgcgatgatg ccgagcatca
      421 tgtagggccg gcgcttgccc gagcggtcgg agagggccgg gatcgcgacg gcgccgagga
      481 tgccgccgac caggaggagg gcgccgaagg tcccggcctc ctgggggccg aagcccctcg
      541 gcctcacgat ctcctcgatc caagtggtca ccccgttgaa gatgcccatg cccacgaaga
      601 ccacgccaag gaagaggcgg aaggagggca ccctgagcgc gtgcttgacc ccgtcgaaca
      661 tcagggcccg ctcatcggcc tcgacgggcc cgggggggct cggcggggac tcgcgcgcgg
      721 ccaggaggaa ggccagggtc gagagggcgg cgaagcccga gaagatatac tgggtccccg
      781 ggatcccgac gatgggcgcg agcatgggcg tggccaccat gcctccggcg atgccgagga
      841 ggttggagag ggtgatgagg cccacggccg tcgccctttc gcccctcggg aaccagtggg
      901 cggggagctt ggtccaggcg ttgaggaaga ggggctgggc cacggcgagc ccgaggcttc
      961 ccgtgagcgc gagggcatag ctcgtgccgg cgaagccgcg cagggccccg aagacgacca
     1021 tcatgatcga agcgaggccg gcggccttct tgaagccgta gcggtcgatg gcccaggacg
     1081 cggggatgga gaggggcaca tagacgatca tgaagaccat ggccaggaag ccgatcttga
     1141 gctcggacac cccgaagaag cgggcggcgt cggcggtgat gggggcatag gagatccaga
     1201 gcacctggat gatgaaattc acgagcatga aggcgccgag cacgacccag cggtagccgt
     1261 agattcgata ttctgtgtct cgcacgaggg actccggcct ttcgaggccc cacggaggga
     1321 cctcctgttt ggactccaga aaatattact ggttccctca agatatacaa gcgcccgggg
     1381 caaaaaagtg ggcgggcgcg gccaatggcc acgcccgccc cttctgcgcc ttgatgctct
     1441 gcctacttct tcttcgcgaa ggcctcatag ttcgcgctcg ggtcgtaggc gccctggcgg
     1501 acctcgcccc cgatgctcgg tatcctgagc acctggccgg gaaggatcag gttgggatcg
     1561 ctcgggtccc tcaggatctt cttgttggcc tcgtagagca ccgtccactg gtaggggtcg
     1621 ttgtagatga aggggtagcc ggcgatgcgc cagagacagt cccgcagcga gggtatgagc
     1681 ctcaccctgt agctcgccgg cagggggctc gaggcctcca ccagggtcag ggcgtcgagg
     1741 acgtccctcg ccctgatcgc ggcctcccgg aagtcctggg cgccgtaggc cgccagggcc
     1801 ttgtccatgc tcgagctcgc ccggctgtag tcctcggggt acctcttctg cgcgcccacg
     1861 ccctcggccc aggccagcct ctccttcgcc gcggcgatgg cggcgtcggc gtcggccacg
     1921 gcgatcgcat tgacggcggc caccacggcg tcggcctggt ccttggcggc ggcatagtcc
     1981 tcggcggcgt aggccttggt cgccgcgtcc atcccgtcct tggccttggc gtagagctcg
     2041 gggaagctgg cctgggcccc cctgccgtcg gcccaggcga gctgggcctt ggcgaggccg
     2101 atggacttgt tcgcggccgt gagcgcgagc atcttctcga catagcggtc cgagagcagg
     2161 gcgttctcct tggcctgggc ggcgaggctg gcggccgcgt cgtagtcgcc cgcgtcgaag
     2221 gccttctgcg cctgggcgct caggtccagg ctccgtctgt agaacgagtt gtcgatgagg
     2281 ctctgcgcct ggagggcgag ggccgacgcg aggatcgtca tcacgacgac cagtgctgtc
     2341 cttctcatgt cgccctcctt actgggccgc ctggctctgg ttcacgatgg ggtcggcctg
     2401 ggccgccttc ttctcgctct cgccggcggc ctcctgggcc gccttcatcg cggcctcggc
     2461 cgccgcgcgc ttcgccgccg ccgcgtcgta ggcggcgtcg aagtcggtcc ccgacttctg
     2521 gagggcggcc gtggccccct cgtagtcgcc ggaggtcagc tttgagatgc cctcctcgta
     2581 gccggcgagg gcggcgtcat acaggtcctt gaccgccacc tgggccttta tggtctcgga
     2641 cctggccttg gactggtcgc tcttctcctt ggcccct
//
LOCUS       DASSQV010000263         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_560422, whole genome shotgun sequence.
ACCESSION   DASSQV010000263 DASSQV010000000
VERSION     DASSQV010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560422"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VFL04_04495"
     CDS             complement(<1..869)
                     /locus_tag="VFL04_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838993.1"
                     /translation="MWKPASTLVIGVAAIALVAGGAVAGDYPLTGTQTGPGLASARTE
                     LMILSAALSPRSLAGAGPEFAAAAARALVRSADLSGVTAKEAMESGLPKPDAVLAMLA
                     LDRDVDDFLESSALGGPVGAHYRKAAELAGRYRPLRAAYEAAYASMEREIAAAPTRDP
                     RPKRIQAQKGKDPLFDRGHFLRPTMHDIRFAHPYALDVFFDHYKQGADGQTGPSIPSI
                     SGGLVVAAAGDWRGGPGPQAWAGGGLSPAAGNGLVVYDPETRRYYSYFHLRSLSVACG
                     ELVERGRILGTGG"
     gene            complement(923..2179)
                     /locus_tag="VFL04_04500"
     CDS             complement(923..2179)
                     /locus_tag="VFL04_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7838994.1"
                     /translation="MSPGPGSARPSAAPLFAFSDAEAVEIAEQHGTPCFAYRLDLAEA
                     RFRALRSVLSDRVRLAFAVKANPHPALLARFAELGSAFDCASGGELERVREAGAPGGS
                     ILFAGPGKARGELRLALDLGARIQADGPEDLERIEGLLAEEPGAGARAPLSVNLRVHP
                     RGGVSEGEGGRGNIIGGTGPSAFGVDEEELLAFLDAAKAFRGIRISGLQVFAASNELD
                     AGRLLANHRTALAIGRAMAERGFELDCIDLGGGLGIPYAEGAPELDIGALGAGLSKLL
                     AENPWFKGSLILEPGRWLAGPLGVYLARVLRAKESRGKRFLVLEGGINHLIRPLLTGQ
                     AFPAKAPGAAGAAVPQVLAGPLCTSLDRLGELPLPPLRAGDLVMLGQAGAYGFTEAMG
                     AFLSHPAPAEHWFEGPPSPSGHQQSS"
     gene            complement(2176..>2908)
                     /locus_tag="VFL04_04505"
     CDS             complement(2176..>2908)
                     /locus_tag="VFL04_04505"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET7838995.1"
                     /translation="ELLGELERGGIRAALRGEDGVWRAQAWVKLAILAGFRSTRTVPI
                     EGWPGGAFDRSAFPPRRLGLEDGVRLVPGGSSVRRGAYLGPGVVIMPPAYVNVGAWVG
                     AGTMVDSHALVGSCAQIGERVHLSAAAQVGGVLEPAGARPVVVEDDCFIGALCGLFEG
                     VVLRRRAVLAPGLILTASTTVFDLVGERELSGEIPEGAVVVPGSRPAKGGFARERGIS
                     LYVPCIVKYRDQGTDAATALEGALR"
BASE COUNT          423 a         1019 c         1078 g          388 t
ORIGIN      
        1 ttgccccccg tgccgaggat ccggccgcgc tcgacgagct cgccgcaggc gacggagagg
       61 gagcggaggt ggaaatagga gtagtagcgc ctggtctccg ggtcgtagac cacgaggccg
      121 ttgcccgcgg ccgggctcag gcccccgccg gcccaggcct gggggcccgg cccgccccgc
      181 cagtctccgg cggcagccac gacgaggccc cccgagatcg aggggatcga gggccccgtc
      241 tggccgtcgg cgccctgctt gtagtggtcg aagaagacgt cgagggcata ggggtgggcg
      301 aatctgatgt cgtgcatcgt gggcctcagg aagtgccccc gatcgaagag tgggtccttg
      361 cccttctggg cctggatccg cttcgggcgg ggatccctcg tcggggccgc ggcgatctcg
      421 cgctccatcg aggcgtaggc cgcctcgtag gcggcccgca ggggccggta tcttccggcg
      481 agctcggcgg ccttgcggta gtgggctccg accggtccgc ccagggcaga ggattccagg
      541 aaatcgtcga catcgcggtc gagggcgagc atggccaaga cggcatccgg tttgggcagg
      601 cccgattcca tggcctcctt ggcggtgacc cccgagaggt cggcgctgcg caccagggcc
      661 cgggctgcgg ccgcggcgaa ctcgggccct gcccccgcga gcgagcgggg ggaaagggcc
      721 gccgacagga tcatgagctc ggtgcgggcc gatgccaggc caggacctgt ctgcgtgccg
      781 gtgagcggat agtcccccgc gaccgcgccg cccgctacaa gggcgatggc cgccactccg
      841 atgaccaagg tgctggcagg tttccacatc ggtctaggat catagccgtc cccggccttc
      901 ctcgtccacg cctaataggg tgtcacgaag attgttggtg gccggagggg ctgggcggcc
      961 cctcgaacca gtgttcagcc ggggcagggt ggctcaggaa ggcccccatc gcctcggtga
     1021 agccataggc cccggcctgt ccgagcatga ccaggtcccc ggccctgagc gggggaaggg
     1081 ggagctcgcc gaggcggtcc agggaggtgc agagggggcc ggcgaggacc tgggggacgg
     1141 cggcgccggc ggcgcccggg gccttggcgg ggaaggcctg tcccgtgagg agggggcgga
     1201 tgaggtggtt tatcccgccc tcaaggacga ggaagcgctt gccccggctt tccttcgccc
     1261 tgagcacccg cgccagatag acaccgaggg ggccggcgag ccagcgaccc ggctcgagga
     1321 tgagtgagcc cttgaaccag gggttctcgg ccaggagctt ggagagcccg gccccgaggg
     1381 caccgatgtc cagctcaggg gctccttcgg cataggggat accgaggccc ccgccgaggt
     1441 cgatgcagtc gagctcgaat ccgcgctcgg ccatggccct tccgatcgcg agggctgtcc
     1501 tgtggttggc gaggaggcgg ccggcgtcga gctcgttgct cgcagcgaag acctggaggc
     1561 cgctgatcct gatccccctg aaggccttgg ccgcgtcgag gaaggccaag agctcctctt
     1621 cgtcgacccc gaaggccgag ggccccgtgc cgccgattat gttgcccctc ccgccttccc
     1681 cctccgacac gccgcctcgg gggtggaccc gcaggttgac ggaaagggga gcccgagccc
     1741 cggcgccggg ctcttcggcc aggaggcctt cgatccgctc aaggtcctcg ggcccatcgg
     1801 cctggatcct cgcgccgagg tcgagggcga gcctgagctc gccccgggcc ttgcccggcc
     1861 ccgcgaagag gatgctcccg cctggggccc cagcctccct gacccgctcg agctcccctc
     1921 cggaggcgca gtcgaaggct gagccgagct cggcgaagcg ggcgaggagg gcggggtggg
     1981 ggttggcctt gaccgcgaag gcgagacgga cgcggtcgct caagaccgag cggagggcac
     2041 ggaaccgcgc ctcggccaga tcgagacgat aggcgaaaca gggcgtgccg tgctgctcgg
     2101 ctatctccac cgcctcggcg tcggagaagg cgaagagggg ggccgcgctg ggcctggcgg
     2161 agccggggcc cgggctcacc gcagggcccc ctcgagggcc gtggccgcgt cggtgccctg
     2221 atcacggtat ttgacgatgc acggaacata gagcgatatg ccccgctccc gagcgaagcc
     2281 gcccttggcc gggcgcgagc cggggacgac gaccgccccc tcgggtatct cgcccgagag
     2341 ctcgcgttcc ccgacaaggt cgaagacggt ggtcgaggcc gtgaggatga ggccgggagc
     2401 gaggacggcc ctccgcctca ggaccacgcc ctcgaagagc ccgcagagcg cgccgatgaa
     2461 gcagtcgtcc tcgaccacga ccggcctcgc tcccgccggc tcgaggaccc cgccgacctg
     2521 ggcggcggcc gagaggtgga ccctctcccc gatctgggcg caggacccga cgagggcgtg
     2581 gctgtccacc atggtccccg cccccaccca tgcccccacg ttgacgtagg ctgggggcat
     2641 gatgacgact ccggggccca ggtacgcccc ccgccgcacc gagctgcctc cgggcacgag
     2701 cctcaccccg tcctcgaggc cgagccgtct cggggggaag gccgacctgt cgaaggcccc
     2761 gccgggccag ccctcgatcg ggaccgtcct cgtcgagcgg aaaccggcga ggatggcgag
     2821 cttgacccag gcctgggccc tccatacccc gtcctctccc cgcagggcgg ccctgatccc
     2881 gcctcgctcg agctctccaa ggagctcg
//
LOCUS       DASSQV010000264         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2177467, whole genome shotgun sequence.
ACCESSION   DASSQV010000264 DASSQV010000000
VERSION     DASSQV010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2177467"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1837
                     /locus_tag="VFL04_04510"
     CDS             167..1837
                     /locus_tag="VFL04_04510"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7838996.1"
                     /translation="MREDRDRPPGADGSGGDGSGGDLVIVKARIHCHGRRDWLPALAV
                     RGGRIVCAGSEAEARGSVAGPVLDLHGACVLPGLVDAHIHFEDLSLGLGRVNVDAPSL
                     EEALARVKLKAGSTPKGTWILGQGWNANAWGPSQPKAAMLDALAPDRPVCLVDKSCHA
                     VWVNSTALALAGIGPGSPDPAEGRIVRDAGGVATGILLEAATHLVTDLVPVPTAAELE
                     AAFKAGALEALRHGLTGVHDFDGPRSFSTQASLRAKGALGLRVLKSIPYELLDQALEL
                     GLRSGFGDDMLAFGQLKLFADGAMGPQTAWLLADYEGRPGYRGMGLTPIADIRRAILR
                     ANAAGIACAVHAIGDRANREVLDAYESVARELGPGRMAALAMPNRIEHAQILDPADRP
                     RLAGLGLVASMQPIHATSEIELTELHLGSRSGGAYGFGSQARLGAVLAFGSDCPVETL
                     DPIAGIHAAVTRRRRSGEPGPRGWQPDERLGLEEAIQAYSLGPARAAGKADRLGSIER
                     GKLADLSILDRDIFELPPDEILSARPLATVVDGRLVWRSEIERPLGSS"
     gene            1963..2640
                     /locus_tag="VFL04_04515"
     CDS             1963..2640
                     /locus_tag="VFL04_04515"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HET7838997.1"
                     /translation="MTVIAHIRDQILSDRLRPGDRLPSERELCETLKVSRGYVRKAIV
                     KLNHIGLIETLPQKGTIVAGFSGRAISGLLSSIGNLDGSFDPIDLFEVRSNLESFSAR
                     QAALRAGRAELEAIESWHSAFRAKVETGVRALEEDHLFHLAIAKASGNAVCFALVCYI
                     TPQILALAAKGETSTQDRLTNTYEEHDRIVKAILSGDPAGAEAAMREHMEEAWKRRTP
                     DSESKRT"
     gene            2735..>3677
                     /locus_tag="VFL04_04520"
     CDS             2735..>3677
                     /locus_tag="VFL04_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7838998.1"
                     /translation="MKKTLALALVLAMSLALAVFAAPVTITHWYWADSPAFSQTMQKM
                     AADFSATNGKGITVVAQEYPWDGGGYSEMLFRTAMGGGGPDTSSAKLTSTPLFTANKL
                     WVDLEAQLGAWKDRNLINASLYDTIKGASGGKGIFLMPWNTQVLYVYYRPSMLKAAGL
                     SVPKTYAEFLDACRKLTRDTNGDGKIDVYGFGMRGAKGGQEPWGSFIWARGGSFADLT
                     SPKAVQGMQDYIDLYRNGYVPPTAPADGFNEIIANFKSGKTAMTVHHIGSSTSMIDTF
                     GADVDAFAFPGDVGRWTSMGDTENVMLSGCKNRDAAFE"
BASE COUNT          618 a         1314 c         1205 g          540 t
ORIGIN      
        1 aggcccaggc cgaacagctc agacgcttcg gctgcgactt cggccagggc tatctcttcg
       61 gcaagccgat ggactcgggc tccctcgccc tcctcctttc gggggcgctc acccgcatca
      121 gccttgaggg cgccatcacc aaggacccgg ggccggaggc cgagcggtga gggaggacag
      181 ggaccgccct cccggcgctg atggctctgg cggagacggc tccgggggcg atctcgtcat
      241 cgtcaaggcc cggatccact gccatggacg gagggactgg ctgccggccc tcgccgtccg
      301 cggaggaagg atagtctgcg cgggctctga ggccgaggcc agaggctctg tagccggccc
      361 ggtgctcgac ctccacggcg cctgcgtcct tccgggcctc gtcgatgccc acatccattt
      421 cgaggacctc tccctcggcc tgggccgggt caacgtcgac gccccgagcc tcgaggaggc
      481 cctcgcccgc gtcaagctta aggcgggctc gacgccgaag gggacctgga tcctcggcca
      541 gggctggaac gccaacgcct ggggcccctc ccagccgaag gcggcgatgc tcgacgccct
      601 cgctcccgac aggccggtct gtctcgttga caagagctgt cacgcggtct gggtcaactc
      661 gacggccctc gccctcgcgg ggataggccc aggaagcccc gaccccgccg aggggaggat
      721 cgtccgcgac gcggggggag tggcgacggg gatcctcctc gaggccgcca cccatctggt
      781 gaccgatctg gtgccggtgc ccacagcggc cgagctcgag gccgctttca aggcgggagc
      841 cctcgaggcc ctgcgccacg ggctcaccgg ggtccacgac ttcgacgggc cgcgctcctt
      901 ctcgacccag gcctccctgc gcgccaaggg ggccctgggc ctccgcgtgc tcaagagcat
      961 cccctacgag ctcctcgacc aggccctcga gctgggcctc cggagcggct tcggcgacga
     1021 catgctcgcg ttcggccagc tcaagctctt cgccgacggg gccatggggc cccagaccgc
     1081 ctggctcctg gccgactacg agggccgtcc gggctaccgc ggcatggggc tgacgccgat
     1141 cgccgacata cggcgggcca tcctcagggc caacgcggcc ggcatcgcct gcgccgtcca
     1201 cgcgatcggg gacagggcca accgcgaggt cctggacgcc tacgagtcgg tggcccgcga
     1261 gcttgggcca gggcggatgg ccgccctggc catgcccaac cgcatcgagc acgcccagat
     1321 cctcgaccca gccgacaggc caaggctcgc cgggctcggc ctcgtggcct cgatgcagcc
     1381 gatccacgcg acctcggaga tcgagctgac cgagctccat ctgggctcga ggagcggggg
     1441 ggcctatggc ttcggcagcc aggcgcggct gggggcggtg ctggccttcg gttcggactg
     1501 ccccgtcgag accctcgacc ccatcgccgg catccacgcc gcggtcacga ggaggaggcg
     1561 gagcggggag cccgggccga ggggctggca gcccgatgag cggcttgggc tcgaggaggc
     1621 catccaggcc tacagcctcg gcccggcccg cgcggccggc aaggccgaca ggctcggcag
     1681 catcgagcgc ggcaagctcg ccgacctcag catcctggac cgggacatct tcgagctgcc
     1741 gcccgacgag atcctctcgg cccggcccct ggcgaccgtg gtggacggac gcctggtctg
     1801 gcggtcggag attgagcggc ccctcggctc ctcctgaccc acatttcgtg caatttggtt
     1861 tgacaaatcc ggaaaatgcc ctgatactca ggccaatctg gttaaccagt tctggttaac
     1921 cagattggga gcccctggat gatcgtctca ccggattcgg aaatgacggt gatcgcccac
     1981 atccgcgacc agatcctgtc cgacaggctc aggcccgggg acaggctccc ctccgagcgc
     2041 gagctctgcg agaccctgaa ggtgagccgc ggctatgtca ggaaggccat cgtcaagctc
     2101 aaccacatcg gcctcatcga gaccctgccc cagaagggga cgatcgtcgc gggcttcagc
     2161 ggaagggcga tctcgggcct gctctccagc atcggcaatc tggacgggag cttcgatccc
     2221 atcgacctct tcgaggtgcg ctccaacctc gagtccttct ccgcccgtca ggctgccctg
     2281 agggcgggca gggccgagct cgaggccatt gagagctggc actccgcctt cagggccaag
     2341 gtcgagacgg gggtcagggc cctcgaggag gaccacctct tccacctcgc catcgcgaag
     2401 gccagcggca atgccgtctg cttcgccctt gtctgctaca taacgcccca gatactcgcg
     2461 ctcgccgcga agggcgagac cagcacccag gacagactca ccaacaccta tgaggagcac
     2521 gacaggatcg tcaaggctat cctttccggt gacccggccg gcgccgaagc cgcgatgcgg
     2581 gagcacatgg aggaggcctg gaaacggaga acgccggatt cggaatcgaa gcggacctga
     2641 accgcggtcc cagggtcccc ccagggaatt ccgggcgcct cggctgcccg gcttgcgata
     2701 gatgggcatt cggacatcca aggaggttct ggcaatgaaa aagaccctgg ccctggctct
     2761 cgttctcgcc atgtccctgg cgttggccgt cttcgctgcg cccgtgacga tcacccactg
     2821 gtactgggcc gattcgccgg ccttctccca gaccatgcag aagatggccg ccgacttcag
     2881 cgcgacgaac ggcaagggca tcaccgtcgt cgcccaggag tatccctggg acggcggcgg
     2941 ctacagcgag atgctcttcc gcaccgcgat gggcgggggc ggacccgaca cctcctcggc
     3001 gaagctgacc tcgacccccc tcttcaccgc caacaagctc tgggtagacc tcgaagccca
     3061 gctgggcgcc tggaaggaca ggaacctcat caacgccagc ctctatgaca ccataaaggg
     3121 cgcctcgggc gggaagggca tcttcctcat gccctggaac acccaggtcc tctacgtgta
     3181 ctacaggccc tccatgctca aggccgccgg gctgtcggtg cccaagacct acgcggagtt
     3241 cctcgacgcc tgcaggaagc tcacccgcga caccaacggc gacggcaaga tagacgtcta
     3301 cggcttcggc atgcgcggcg cgaagggcgg gcaggagccc tggggcagct tcatctgggc
     3361 caggggcggc agcttcgccg acctcaccag cccgaaggcc gtgcagggca tgcaggacta
     3421 catcgacctg tacaggaacg gctacgtgcc ccccacggcc cccgccgacg gcttcaacga
     3481 gatcatcgcc aacttcaagt ccgggaagac cgcgatgacc gtccaccaca tcggctcctc
     3541 gaccagcatg atcgacacct tcggcgctga cgtcgacgcc ttcgccttcc ccggcgacgt
     3601 gggccgctgg acctcgatgg gcgacaccga gaacgtgatg ctgtccggct gcaagaacag
     3661 ggacgcggcc ttcgagt
//
LOCUS       DASSQV010000265         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2426558, whole genome shotgun sequence.
ACCESSION   DASSQV010000265 DASSQV010000000
VERSION     DASSQV010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2426558"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VFL04_04525"
     CDS             complement(<1..865)
                     /locus_tag="VFL04_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7838999.1"
                     /translation="MGNGKLTLHVEGMSCSSCEARIEKALLAVPGVTRAKADQQGGRV
                     VLEYDEGLASPEAARAAIEKAGYAVRDKRNASTLIALGIGLVLASAYMIASSAGLFAS
                     LPKIDASIGYGMLFVVGLLTSVHCVAMCGGIALSQSVKALPRGADGDGSRPRPFGRDL
                     RRLVPGLRYNGGRVISYTVIGGLVGALGSAFDFSPAVKGGIAGAAGLFMLYLGLKMLG
                     LVPAMPRLSRLLPASAARRIEAARQTAAGRGPFAVGLLNGLMPCGPLQTMQLYALGTG
                     SLFAGALSMLIF"
     gene            1081..>2130
                     /locus_tag="VFL04_04530"
     CDS             1081..>2130
                     /locus_tag="VFL04_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET7839000.1"
                     /translation="MRESGGKTVLVVEDDAVSALVCADRLRSFGYRSLVAGTGQEAVE
                     LALGSGRVDLVLMDIELGEGMDGAEAARRILAARELPIVFHTAHAEQGYVDRVRQIAR
                     YGYVTKNSGDFVLRSSIEMAFELFESHASIAAKVAELRGHEAFLDILLETVPLPLFVK
                     DRAGVYIRANKAYAEFMGMDLAAVLGKDVFGIYPPDLAEVYRRHDDELYRDGGIQTYE
                     SKVQISDGSRRDILVCKAVFRDQGGEAAGIIGVVEDVTERTRAQERVQSLLAEKDLIL
                     REVHHRIKNNMKTMEALLSLQADSQTEASARAALDDARGRIQSMIVLNDTLSRSEGSG
                     PVQLRDYLVELLDRVL"
BASE COUNT          352 a          717 c          757 g          304 t
ORIGIN      
        1 agaagatgag catggagagg gcgcccgcga agaggctccc ggtcccgagg gcgtagagct
       61 gcatggtctg cagggggccg cagggcatga gcccgttgag gagcccgacc gcgaaggggc
      121 cgcgtcctgc cgcggtctgc cgcgccgcct cgatccgcct cgctgccgag gccggcagga
      181 ggcgcgagag ccggggcatg gcggggacca ggccgagcat cttcaggccg aggtagagca
      241 tgaagagccc cgcggcgccg gcgatgcctc ccttgacggc gggcgagaag tcgaaggccg
      301 agccgagggc gccgacgagg ccgccgatga ccgtgtagga gatgacccgc ccgccgttgt
      361 accggaggcc ggggacgagc cgcctgagat cgcggccgaa ggggcggggc cggctcccgt
      421 ccccgtcggc gcccctgggg agggccttga cgctctgcga cagggcgatg ccgccgcaca
      481 tggccacgca gtgcaccgag gtgaggaggc ccaccacgaa gagcatgccg tagccgatcg
      541 aggcgtcgat cttgggcagg gaggcgaaga gccccgccga gctggcgatc atgtaggccg
      601 aggccagcac gaggccaatg ccgagggcga tgagggtgct cgcgttccgc ttgtcccgga
      661 ccgcgtagcc ggccttctcg atcgccgccc tggcggcctc cggagaggcc aggccctcat
      721 cgtactcgag gacgacccgc ccgccctgct gatcggcctt ggcgcgcgtg acgccgggga
      781 cggcgagcag cgccttctcg atccgggcct cgcaggatga acagctcatc ccctcgacat
      841 gcagtgtcag cttcccattc cccatcgcat tggcctcctc ggctatcgag gcagcccggg
      901 tcggcgcctc cgtgacgacg agaatctagc aggcccgtgt gaatggagtg tgaacgcgct
      961 ctgtttcttc catggcttgg cccagtccag gcccctcccg ggcgttcctc ccggcttttt
     1021 gccactggcc aagccagagc tggtcggcga tactttcgca agctggggag gtcggcggct
     1081 atgcgtgagt cgggagggaa gaccgtgctg gtggtcgagg acgatgccgt gtccgcgctc
     1141 gtgtgcgcgg acaggctcag gtccttcggc taccggagcc tcgtggccgg gacggggcag
     1201 gaggcggtcg agctcgccct gggaagcggg agggtcgacc tcgtcctcat ggacatcgag
     1261 ctgggcgagg gcatggacgg ggccgaggcg gcgaggagga tcctcgccgc gagggaactg
     1321 ccaatcgtct tccacaccgc ccacgccgag cagggctacg tggaccgggt gaggcagatc
     1381 gcccgctacg gctacgtgac caagaactcg ggcgacttcg tcctgcgctc gtccatcgag
     1441 atggccttcg agctcttcga gtcccatgcg agcatcgccg caaaggtggc cgagctccgc
     1501 ggccacgagg ccttcctcga catcctcctc gagaccgtgc ccctgcccct gttcgtgaag
     1561 gacagggccg gggtctacat ccgcgccaac aaggcctacg ccgagttcat gggcatggac
     1621 ctggcagcgg tcctaggcaa ggacgtcttc ggcatctacc cgcccgacct ggccgaggtc
     1681 taccgccgcc acgacgatga gctctaccgc gatggcggga tccagaccta cgagagcaag
     1741 gtccagatca gcgacggcag ccgccgcgac atcctggtct gcaaggcagt gttccgggac
     1801 caaggcggcg aggctgcggg gatcatcggc gtggtcgagg acgtcaccga gcgcacgagg
     1861 gcccaggagc gggtgcagag cctcctggcc gagaaggacc tcatcctgag ggaggtccac
     1921 caccgcatca agaacaacat gaagacgatg gaggccctcc tctccctcca ggccgacagc
     1981 cagaccgagg cctcggcgag ggccgcgctg gacgacgccc gcggccgcat ccagagcatg
     2041 atcgtcctca acgacaccct ctcccgctcc gagggctcgg gccccgtcca gctccgggac
     2101 taccttgtgg agctcttgga ccgggtcctc
//
LOCUS       DASSQV010000266         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_437982, whole genome shotgun sequence.
ACCESSION   DASSQV010000266 DASSQV010000000
VERSION     DASSQV010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437982"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="VFL04_04535"
     CDS             <1..1553
                     /locus_tag="VFL04_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET7839001.1"
                     /translation="GTSAVVLDRFGFGVIAEARVGYRDDPRFACLGLGAESPILPPGE
                     PGEAHQPVAVFLAGLEAVLEDLPRAFLSRVAAIDLSAQQHGQVWLGAEGIKAISALAR
                     PGAGAPGMPSLASSLGPGLAWERSPIWMSSNTAAEASLLRRALGGAEAVTARSGSDVP
                     LRFSGPVLIRSARRYPQAWGRTRRFHLISSFLSGVLAAKPDCPVDWGNGSGTGLMDWK
                     RRAWDGELIAAAARGMGCPADRLASVLPALAHPLTIAGPIAAYFAERYGFDPACRVLV
                     GSGDNPQTKVLASGCLLSLGTSFVLMAEGDRPHVSANAMYDGLGRPFVFGCRTNGALS
                     WEAIRRRHGLEPGDYASSDRALAMSPPGADFRILQTESESFPVSPPLDLGACGDFAAD
                     YAGVVDASLCLLYLGSKPFAKAGGIAATGGGAGSQAVLSRIASIWRARVTPIANAGAA
                     TGAALAAALALRSEKGRRSVIEEARSAAAGARTPVEPDPALVRAYHRPGGFLARLEAK
                     ARSLGFSL"
     gene            1967..2887
                     /locus_tag="VFL04_04540"
     CDS             1967..2887
                     /locus_tag="VFL04_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7839002.1"
                     /translation="MKKVLLIAVAVLMIAPLAFSAGKLKMGVTIEDFNDVFMRYALDQ
                     LKDEAAKNNIDIIAADGKRDPNEQIKQVENFITQGVDAILIHVIDQTIAPRITKLCLD
                     AKIPLVYFNRKPDDSALVAGKAVAVASPEEVAGSLEAEFVAKKLNGKGNVAILMGTMG
                     SAPQIGRTKGSKDVIAKYPDMKVVREQTANFQRPDAVTVVENWLASGVPIDAIIANND
                     EMAIGAVLVIEEKKLQDKILVTGVDATSDAMKFIADKRLDMTVFQNGGAQGRGAVQTA
                     LKLLNHESVPVFTAIPFEPVTYDNYRNYMK"
     gene            2989..>3877
                     /locus_tag="VFL04_04545"
     CDS             2989..>3877
                     /locus_tag="VFL04_04545"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET7839003.1"
                     /translation="MSDYILQMKGIHKSFPGVQALRGVDLSVRPGSVHGVMGENGAGK
                     STLMKIAIGLYQPDAGEISLRGQKVSFESVHDALSRGISMIHQEMSPLPYMTVAQNIF
                     LGREMRNALGLIDHRAIDREAERMLAELEINLGPRDIMGRLSVAQMQLVEIAEAVSRK
                     ADLIIMDEPTSALTEKEVAHLVSIIRGLKARGASVIYITHKIDEVFAITDEVSVYRDG
                     EFVGHEMSSDLTREKLIKMMVGRTIDQFFIKEKAAFGSPVLEVEGLTRPGYFRDISFS
                     VREGEILGVAGLIGAGRTEV"
BASE COUNT          668 a         1361 c         1272 g          576 t
ORIGIN      
        1 agggcacgag cgccgtcgtc ctcgaccgct tcggcttcgg cgtcatcgcc gaggccaggg
       61 tcggataccg cgacgatccc cggttcgcct gcctcgggct cggcgcagaa agcccgatcc
      121 tgccgcccgg cgagccgggg gaggcccacc agcccgtcgc cgtcttcctc gccgggcttg
      181 aggctgtcct cgaggacctc ccccgcgcct tcctctcacg ggtcgcggcg atcgacctct
      241 cggcccagca gcacggccag gtctggctcg gcgccgaggg gatcaaggcg atctcggccc
      301 tggcaaggcc gggggctggc gcgccgggta tgccatcgct cgcatcatcc ctgggccctg
      361 gcctcgcctg ggagcgctcc ccgatctgga tgagctcgaa cacagcggcc gaggcctcac
      421 tcctgcgcag ggccctcggc ggggccgagg cggtgacggc gcgctcgggc tccgacgtcc
      481 ccctgcgctt ctcgggcccc gtcctcatcc gctcggccag gcgctatccc caggcctggg
      541 gccgcaccag acgcttccac ctcatctcat ccttcctctc gggcgtgctc gccgccaaac
      601 ccgactgtcc cgtggactgg ggcaacggct cagggaccgg cctcatggac tggaagcgca
      661 gggcctggga cggggagctc atcgcggcgg ccgcgcgcgg catgggctgt cccgccgatc
      721 ggctcgcctc cgtccttccg gccctcgccc atcccctgac catcgcgggt ccgatagccg
      781 cctatttcgc cgagcgctac ggcttcgatc cggcctgccg cgtcctcgtc ggctcgggcg
      841 acaacccaca gaccaaggtc ctggcctcgg gctgcctcct ctccctcggc accagcttcg
      901 tcctcatggc cgagggggac agaccccacg tctcggccaa cgcgatgtac gacggcctgg
      961 gcaggccctt cgtcttcggc tgcaggacga acggggccct cagctgggag gccatcagga
     1021 ggaggcatgg cctcgagccc ggcgactacg cctcctcaga cagggccctg gcgatgagcc
     1081 cgccgggcgc cgacttcagg atcctccaga ccgagagcga atccttcccc gtctcgccgc
     1141 ccctcgacct gggagcctgc ggagacttcg ccgcggacta cgccggggtc gtcgacgcct
     1201 cgctctgcct cctctacctc ggttcaaaac ccttcgccaa ggccggcggc atcgccgcga
     1261 ccggcggcgg ggcggggagc caggccgtcc tctcgcgcat cgcctcgatc tggagggcga
     1321 gggtcacgcc catagccaac gcgggcgcgg ccacgggggc cgccctcgcc gccgccctcg
     1381 ccctcaggtc cgaaaagggg aggcgctcgg tcatcgagga ggcccggagc gccgcggccg
     1441 gggccaggac ccccgtcgag cccgatcccg ccctcgttcg ggcctatcat cgcccggggg
     1501 gcttcctcgc ccgtctcgag gccaaggcca ggagcctcgg tttcagcctc tagcggccgg
     1561 gcgggggaca gtcaccggga agcaccgggc agggacagtc accggaccgg ggacagtcac
     1621 cgggcggtgg ccggtcgcgg ccgcggatct tgtattgcca gagcccgggg ccgggcgtag
     1681 aatcggccta ggaggggtgg gcggtccagg ccgagcacgc ggccggaccc cgaaccgggt
     1741 cccgagccca tttttccgga cttgccttgg cgccgaggag cggctagcat atctttacac
     1801 catgggcaaa ggaggtgagg aggagacgac acgttggctg gacgcgtggc ctcggagcta
     1861 atggagggat ttcatcaata aggaactcta gaggaatcgg cgcaatttct ggtgattgcc
     1921 ggcgtccgcg tggcgggcgc gaggatttct tcaaggaggg tttcaaatga agaaagttct
     1981 tctcatcgcg gtggccgtcc tcatgatcgc gccactggcc ttctccgccg ggaagctcaa
     2041 gatgggggtc accatcgagg acttcaacga cgtgttcatg cgctacgccc tcgaccagct
     2101 caaggacgag gccgcgaaga acaacatcga catcatcgcg gcggacggga aacgggatcc
     2161 caacgagcag atcaagcagg tcgagaactt catcacccag ggggtggacg cgatcctcat
     2221 ccacgtcatc gaccagacga tcgcccccag gatcaccaag ctctgcctcg acgccaagat
     2281 ccccctcgtc tacttcaacc gcaagcccga cgattccgcc ctcgtcgcgg gcaaggccgt
     2341 cgccgtcgcc tcgcccgagg aggtcgctgg cagcctcgag gccgagttcg tggccaagaa
     2401 gctcaatggc aagggcaacg tcgccatcct catgggcacc atgggctcgg ccccccagat
     2461 cggccggacc aagggcagca aggacgtcat cgccaagtac cccgacatga aggtcgtccg
     2521 cgagcagacc gccaacttcc agcgaccgga cgccgtcacc gtcgtggaga actggctcgc
     2581 cagcggggtg cccatcgacg cgatcatagc caacaacgac gagatggcca tcggggccgt
     2641 cctcgtcatc gaggagaaga agctccagga caagatcctc gtcaccgggg tcgacgccac
     2701 ctccgacgcg atgaagttca tcgccgacaa gcgcctcgac atgacagtct tccagaacgg
     2761 aggggcccag ggtcgagggg cggtccagac ggcgctcaag ctgctcaacc acgagagcgt
     2821 gccggtgttc accgcgatcc ccttcgagcc cgtcacctac gacaactaca ggaactacat
     2881 gaagtagtcg gcccaattcc gagggcgcgc aaagagaagg caaccacgga ggcgcggagc
     2941 tccgtggttg cctgcatccg gccctcgtcc ggcaaaggca ggcgcagctt gagcgactac
     3001 atcctgcaga tgaagggcat ccacaagagc ttccccgggg tccaggccct caggggcgtc
     3061 gacctttcag tcaggcccgg ctcggtccac ggggtgatgg gcgagaacgg ggccggcaag
     3121 tcgaccctca tgaagatagc catcggactc taccagccgg acgccggcga gatcagcctg
     3181 cgcggccaga aggtgagctt cgagagcgtg cacgacgccc tctcgcgggg gatctcgatg
     3241 atccaccagg agatgagccc ccttccctac atgaccgtcg cgcagaacat cttcctcggc
     3301 cgcgagatgc ggaacgccct cggcctcatc gaccaccgcg cgatcgacag ggaggctgag
     3361 cggatgctcg ccgagctcga gatcaacctg gggccccgcg acatcatggg caggctcagc
     3421 gtggcccaga tgcagctcgt ggagatcgcc gaggccgtct cgcgcaaggc cgacctcatc
     3481 atcatggacg agccgacctc ggccctgacg gagaaggagg tggcccacct cgtctcgatc
     3541 atcaggggcc tcaaggccag gggggcctcg gtcatctaca tcacgcacaa gatcgacgag
     3601 gtcttcgcca tcaccgacga ggtgagcgtc taccgcgacg gggagttcgt cggccacgag
     3661 atgagctcgg atctgacgcg cgaaaagctc atcaagatga tggtgggccg cacgattgac
     3721 cagttcttca tcaaggagaa ggcggccttc ggatcgcccg tcctcgaggt cgagggcctc
     3781 acccggccgg gctacttccg cgacatctcc ttctcggtgc gggaggggga gatcctcggc
     3841 gtggccggcc tcatcggggc gggcaggacc gaggtga
//
LOCUS       DASSQV010000267         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2178285, whole genome shotgun sequence.
ACCESSION   DASSQV010000267 DASSQV010000000
VERSION     DASSQV010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2178285"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFL04_04550"
     CDS             <1..755
                     /locus_tag="VFL04_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7839004.1"
                     /translation="SKLLSDSEWAPRFRGALRNNFVFFAIHMLVQNPIGLLLAALLVG
                     KGKARSVYRTLLFLPTVLSVVIIGFIWQLILSPLWGVAPSMLKAVGMASLFKPWLGLS
                     QSALITVSLISVWQFIGIPMILFYTALIGIPDDLVEAAVVDGCTPWNIFWRIKLPLVL
                     PTLGVVAVLTFVANFNAFDLIYTMKGALAGPEFSTDIMGTLFYRTFFGQQLQLGNPTM
                     GATVAAMMFLIIAAGVLAYIFLWQRRVQSYQL"
     gene            765..1607
                     /locus_tag="VFL04_04555"
     CDS             765..1607
                     /locus_tag="VFL04_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7839005.1"
                     /translation="MRTLGLGRRLRESGDFLILSFFALLALFPIWMILTNSFKARKFI
                     FGAPFSFPDAETFSLIGYQTVLSRSNLLQNFSNSLIVTVSSLALILLLGSMAAYALSE
                     YRFKGNTFLGFYLAIGIMIPIRLGTVGILRLMSALGLVNQLAGLILIYIAQGLPMTVF
                     ILSNFMSQVPDELKQAARIDGASEYRIFGLVIPLVRPALGAIGIFTMIPIWNDLWFPL
                     IVAPSGKTATVTLGVQQFLGQFVSDWNAVLSSLSMAMVPVILLYLLFSRQMIRSITAG
                     ALKS"
     gene            1617..2774
                     /locus_tag="VFL04_04560"
     CDS             1617..2774
                     /locus_tag="VFL04_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HET7839006.1"
                     /translation="MLLLHTADLHLGRTLHERELLEDQGWALEAMVRAIAERKPAALL
                     IAGDIYDRSIPTPEAIGLFDSFLAKALAARPGLVVVAIPGNHDSAARMAFGAGLFRGA
                     GVHFRTRPGDCDEPIVVEAAGERVILWALPFLGPGAFADSEAGGPDGAAGMGSQAELF
                     AEGVGRIRARLEPGVCNVLVAHCFAAGGATSESERGFVGLAEQVDLGLLESFDYSALG
                     HLHRMQRPTERSAYPGSPLAYSFGESGQEKGFLLVEVGAGGSRTEFVPVKPLRRMQRI
                     EGPFSELSAPGAHGEHREDFIEARLTDPLPVLNPADPLRANFPNLLSVRQAAFELQAP
                     STTAVEREERGLGAAMEDYLAFSEDMRGSAPSPAELEVFSKLLAEAERAAD"
     gene            2761..>3092
                     /locus_tag="VFL04_04565"
     CDS             2761..>3092
                     /locus_tag="VFL04_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7839007.1"
                     /translation="MRPIRLVLENFGPYRLRAEVDFSRLGPVFLVWGKTGSGKTSLFD
                     AMTYALYGEAPGVRAGLERQLWSHHAKPGDKPLVEFEFALGGDAYRVVRSPPYKRLNR
                     KAELSEVPP"
BASE COUNT          455 a         1161 c          908 g          568 t
ORIGIN      
        1 actcgaagct cctcagcgac agcgagtggg caccgcgctt ccgcggggcg ctgcggaaca
       61 acttcgtctt cttcgcgatc cacatgctgg tccagaaccc gatcggcctc ctcctcgccg
      121 ccctcctcgt gggcaagggc aaggcccgca gcgtctaccg gaccctcctc ttcctgccca
      181 ccgtgctttc cgtcgtcatc atcggcttca tctggcagct catcctgagc cccctctggg
      241 gggtcgcgcc cagcatgctc aaggccgtcg gcatggcctc cctgttcaag ccctggctcg
      301 gcctctcgca gtccgccctc atcaccgtat ccctcatctc ggtctggcag ttcatcggaa
      361 tcccgatgat cctcttctac acggccctga tcggcatccc cgacgacctc gtcgaggccg
      421 ccgtcgtcga cggctgcacg ccctggaaca tcttctggcg gatcaagctc cccctcgtcc
      481 tccccaccct cggcgtcgtg gcggtgctca ccttcgtcgc caacttcaac gccttcgacc
      541 tgatctacac gatgaaaggc gccctcgccg gacccgagtt ctcgaccgac atcatgggca
      601 cactcttcta ccggaccttc ttcggacagc agctccagct cggcaatccc accatggggg
      661 ccaccgtggc cgccatgatg ttcctcataa tcgccgcggg cgtcctcgcc tacatcttcc
      721 tctggcagcg gcgcgtccag agctaccagc tataggaggc ggggatgcgg acactaggcc
      781 tcggccgccg gctccgtgaa agcggcgatt tcctgatcct cagcttcttc gccctcctcg
      841 ccctcttccc catctggatg atcctcacga actccttcaa ggcaaggaag ttcatcttcg
      901 gagccccctt ctccttcccc gacgccgaga ccttcagcct catcggctac cagaccgtcc
      961 tctcgcgctc gaacctcctc cagaatttct cgaacagcct catcgtgacc gtcagctctc
     1021 tcgccctcat cctcctcctg ggctcgatgg cggcctacgc cctctccgag taccggttca
     1081 agggcaacac cttccttggc ttctacctcg ccatcggcat catgatcccg atccggctcg
     1141 gcaccgtggg catcctccgc ctcatgtcgg ccctcggcct cgtgaaccag ctcgccggcc
     1201 tgatcctcat ctacatcgcc cagggcctgc ccatgactgt cttcatattg tccaacttca
     1261 tgtcccaggt cccagacgag ctcaaacagg ctgcgcgcat cgacggggcc agcgagtacc
     1321 ggatcttcgg cctcgtcatc cccctggtca ggcccgccct cggcgcgatc gggatcttca
     1381 ccatgatccc gatctggaac gacctctggt tccccctcat cgtcgcccca agcggcaaga
     1441 ccgccacggt gaccctcggg gtccagcagt tcctcggcca gttcgtgtcg gactggaacg
     1501 ccgtcctgtc ctcgctctcc atggccatgg tccccgtcat cctcctctat ctcctcttct
     1561 cgaggcagat gatcaggagc atcacggccg gcgccctgaa gtcctagggc ggttccgtgc
     1621 tcctcctcca caccgccgac ctccacctgg gcaggaccct ccatgagcgc gagctcctgg
     1681 aggaccaggg ctgggccctc gaggccatgg tcagggccat cgccgagcgc aagcccgccg
     1741 ccctcctcat cgcgggggac atctacgaca ggtccatccc gacgccggag gcgatcggcc
     1801 tcttcgactc cttcctggcg aaggctctcg ccgcccggcc cgggctcgtc gtcgtggcca
     1861 tacccggcaa ccacgactcg gctgcccgca tggcctttgg ggccgggctc ttccggggcg
     1921 ccggggtcca cttcaggacg aggccgggcg actgcgacga gccgattgtc gtggaggccg
     1981 ccggggaaag ggtcatattg tgggcccttc ccttcctcgg cccgggagcc ttcgccgact
     2041 ccgaggcagg aggacccgat ggggcggccg gcatgggttc ccaggcggag ctcttcgccg
     2101 agggggtcgg gaggatcagg gcccgtctcg agccaggcgt ctgcaatgtc ctcgtggccc
     2161 actgcttcgc ggccgggggg gcgacgagcg aatcggaacg gggcttcgtg ggcctggcgg
     2221 agcaggtcga cctcggcctc cttgaaagct tcgattactc ggccctcggc cacctccacc
     2281 gaatgcagag gccaacggag cgctcggcct atccgggctc gcccctggcc tattccttcg
     2341 gcgagtcggg gcaggagaag ggcttcctcc tcgtcgaggt cggagcgggc ggctcgcgga
     2401 ccgagttcgt cccggtcaag ccgctccgaa ggatgcagag gatcgaaggc cccttctccg
     2461 agctctcggc ccccggggcc cacggcgagc accgcgagga cttcatcgag gcgaggctga
     2521 cggaccccct gcccgtcctc aatccggccg accccctgag ggccaacttc cccaacctcc
     2581 tctccgtgcg gcaggccgcc ttcgagctcc aggccccctc gactacggcg gtggagcggg
     2641 aggagcgggg tctgggggcg gcgatggagg attacctggc gttcagcgaa gacatgaggg
     2701 gctcggcccc gagtccagcc gagctcgagg tcttctcgaa gctcctcgcg gaggccgagc
     2761 gtgcggccga ttaggctcgt cctcgagaac ttcgggccct acaggctcag ggccgaggtg
     2821 gacttctccc gcctcggccc cgtcttcctg gtctggggca agacgggctc ggggaagacc
     2881 agcctcttcg acgcgatgac ctacgccctc tatggtgagg cccctggggt gagggcgggg
     2941 ctcgagcgcc agctctggtc ccatcacgcg aagccggggg acaagcccct cgtcgagttc
     3001 gagttcgccc tcggcggcga cgcctaccgc gtcgtgcgga gcccgcctta caagcgcctt
     3061 aacaggaaag ccgagctctc cgaggtcccg cc
//
LOCUS       DASSQV010000268         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2178309, whole genome shotgun sequence.
ACCESSION   DASSQV010000268 DASSQV010000000
VERSION     DASSQV010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2178309"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2111
                     /locus_tag="VFL04_04570"
     CDS             <1..>2111
                     /locus_tag="VFL04_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7839008.1"
                     /translation="LLEAEDSLAPDFFQPERFLLCAVEDVTERVLREKRLMDAKEEAE
                     KATHTKSQFLANMSHEIRTPIQTILGVVDLFRDTELDSEQSEYVHQIRFSADVLLGLI
                     NDILDFSKIEAGKLDLETSTFDLRSCVRDSVDLLVMDAHRKGLEIILDIEESLPALVR
                     GDPGRLRQIIVNLFKNAVKFTSEGGISISLRQVSRSSGPMMRLEVADTGPGVPAEVRD
                     RLFTPFYQGELGMTRKAGGTGLGLAISRHLAELMGGKVGLAPASPRVGSGNEGSVFWL
                     EVPLVAPEYTAPPRPMASGGGARILVVDDHAGARAFAARVARSAGYRVSEAESGEEAL
                     SILRSSAAEPFALCLVDQTMPQMDGWRLASEITGDTTINSARLVLMAPMGTIGAEAKM
                     KLLRWFNGYIYKPLEPLGLLDAMERALSAEVDLEPAERESGPAARESGPEPQAEEPEP
                     RFDAEVLIAEDHEVNRELFSILLGRLGCRVETAEDGVEAVRIGTGRRFDIILMDIFMP
                     NMDGYEATRALRKAGFKGPIVAVTASALKGEREKCLEAGMDDILVKPFKKQDLAALLA
                     RALAPKTSGAAAGQTGAARGDEAAGPATDEGCEADSEVLDWQAAVETFLGQTETVVRL
                     ISRFSEKAGGQLGELATALDARDFPRFRETAHSMKGAAWNLCARRLGDAALSAEEAGR
                     AGDAEAAGAALALLRLEYGLFT"
BASE COUNT          334 a          779 c          714 g          284 t
ORIGIN      
        1 ctcctcgagg ccgaggacag cctggccccc gacttcttcc agcccgagcg cttcctcctc
       61 tgcgccgtcg aggacgtgac cgagcgggtg ctccgcgaga agcggctcat ggacgcgaag
      121 gaggaggcgg agaaggcgac ccacaccaag agccagttcc tcgccaacat gagccacgag
      181 atccgcaccc ccatccagac catcctcggc gtcgtcgacc tcttccggga caccgagctc
      241 gacagcgagc agtcggagta cgtgcaccag atccgcttct cggccgacgt cctcctcggc
      301 ctcatcaacg acatcctcga cttctccaag atcgaggccg gcaagctcga cctcgagacg
      361 agcaccttcg acctgcgctc ctgcgtgcgg gactcggtgg acctcctcgt catggacgcc
      421 caccgcaagg gcctcgagat catcctcgac atcgaggaaa gcctgccggc cctcgtccgc
      481 ggcgatccgg gccggctcag acagatcatc gtcaacctct tcaagaacgc cgtgaagttc
      541 acgagcgagg gcgggatcag catcagcctc agacaagtct cgaggagctc ggggccgatg
      601 atgcggctcg aggtggccga cacgggcccc ggcgtcccgg ccgaggtccg cgaccgcctg
      661 ttcaccccct tctaccaggg cgagctcggc atgaccagga aggcgggggg cacgggcctg
      721 ggcctcgcga tctcgcgcca cctcgccgag ctcatgggcg gcaaggtcgg cctcgccccg
      781 gcctcgccgc gggtcggatc gggcaacgag ggctcggtct tctggctcga ggttcccctc
      841 gtcgcgcccg agtacacggc ccccccgcgg cccatggcct cgggcggcgg ggccaggatc
      901 ctcgtcgtcg acgaccacgc gggggcccgg gccttcgcgg ccagggtagc gcgctcggcc
      961 ggctatcgcg tctccgaggc cgagtcgggc gaggaggccc tctcgatcct ccggagctcc
     1021 gccgccgagc ccttcgccct ctgcctcgtc gaccagacca tgccgcagat ggacggctgg
     1081 cgcctcgcca gcgagatcac cggagacacc acgatcaact cggcccgcct cgtcctcatg
     1141 gccccgatgg gcacgatagg cgccgaagcg aagatgaagc tcctgcgctg gttcaacggc
     1201 tacatctaca agccccttga gcccctgggc ctcctggacg cgatggagcg ggccctctcg
     1261 gccgaggtcg acctcgagcc cgccgagcgc gagagcggcc ccgccgcgag ggaaagcggt
     1321 ccggagcccc aggccgagga gcccgagccc cgtttcgacg ccgaggtcct catcgccgag
     1381 gaccacgagg tcaaccgcga gctcttctcc atactgctcg gccgcctggg ctgccgggtc
     1441 gagaccgccg aggacggggt cgaggccgtg cggatcggca cgggccggcg cttcgacatc
     1501 atcctcatgg acatcttcat gccgaacatg gacggctacg aggcgacgag ggccctgagg
     1561 aaggccgggt tcaagggccc gatcgtggcg gtcacggcga gcgccctcaa gggcgagcgc
     1621 gagaaatgcc tcgaggcggg catggacgac atcctcgtga agcccttcaa gaagcaggac
     1681 ctcgctgccc tcctggcgag ggccctcgcc cccaagacca gcggggccgc ggccggccag
     1741 accggggcgg cgcgcggtga cgaggccgcc ggccccgcca cggacgaggg ctgcgaggcc
     1801 gacagcgagg tcctcgattg gcaggccgcc gtcgagacct tcctcggcca gaccgagacc
     1861 gtggtccgcc tcatctcgcg cttctccgag aaggcgggcg gccagctcgg cgagctcgcc
     1921 accgccctgg acgcaaggga tttcccccgc ttccgcgaga ctgcccattc gatgaagggg
     1981 gcggcctgga acctctgcgc ccgccgcctg ggcgacgccg ccctctcggc cgaggaggcg
     2041 gggcgggccg gggacgccga ggccgccggg gctgcgctgg ccctgttgag gctggagtat
     2101 ggcctcttca c
//
LOCUS       DASSQV010000269         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2426962, whole genome shotgun sequence.
ACCESSION   DASSQV010000269 DASSQV010000000
VERSION     DASSQV010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2426962"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFL04_04575"
     CDS             <1..654
                     /locus_tag="VFL04_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7839009.1"
                     /translation="DASHADLEGRLQEALAELRSLREEMQISQEERQATTEELQSTNE
                     ELQSTNEELTTSKEEMQSMNEELQTLNSELQAKVDELMGASDDMKNLLNSTDIATLFL
                     DEALRVRRFTTQTANITRLIPADLGRRVTDIASELLYPELAADAEEVLRSLVFRVREI
                     QTREGAWYSMRMMPYRTSENVIDGLVMTFTNITRMKQLEAALAGCQAERPPPDGGRP"
     gene            666..1832
                     /locus_tag="VFL04_04580"
     CDS             666..1832
                     /locus_tag="VFL04_04580"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET7839010.1"
                     /translation="MDDEDRAERLRRQAEEAMGRKGPVSKGEEELDARRLVHELRVHQ
                     VELELQNEELRETRARAEESLERFRELFEYAPVPYVSLDRKGTVLQANLAAGSALRVP
                     RSRLVGRPFQHRLPSERRGEFLEFLGKTIDDGRQQPFETTLSDDCDRSLFARIVGLGS
                     DRKPGECRLAIVDLSDREEARLAIERANADIKALMQELNHRVKNNFGVVMSLLGMGEM
                     KVEDPRAKEILLDSRARVMALTQIYERLHLENEVLAIELSGYLEDLVSSLLSTYAVDA
                     SRIRLVTDFESMRLDARKAGLVGLVFSELISNATKYAYGEGEAGELRVSLARREGRIE
                     LAVADDGRGLPEGTDPSTGLGFSIARSLASQLGGRLSLGSGAGKGLEVRLDFPA"
BASE COUNT          376 a          705 c          716 g          282 t
ORIGIN      
        1 gacgccagcc acgccgacct cgagggccgg ctccaggagg cgctggccga gctgcggagc
       61 ctccgcgagg agatgcagat ctcccaggag gagcgccagg ccacgaccga ggagctgcag
      121 tcgaccaacg aggagctcca gtccacgaat gaggagctga cgacctcgaa ggaggagatg
      181 cagtcgatga acgaggagct ccagaccctg aacagcgagc tccaggccaa ggtcgacgag
      241 ctcatgggcg cgagcgatga catgaagaac ctgctcaaca gcaccgacat agccaccctc
      301 ttcctcgacg aggccctcag ggtcaggcgc ttcacgaccc agaccgccaa catcacgagg
      361 ctgatcccgg ccgacctcgg acggagggtc accgacatcg cctccgagct cctctacccc
      421 gagctggcgg ccgacgccga ggaggtgctc cgcagcctgg tcttccgggt ccgggagatc
      481 cagacccgcg agggggcctg gtattccatg cgcatgatgc cctaccgcac gagcgagaac
      541 gtcatcgacg gcctcgtcat gaccttcacg aacatcacgc ggatgaagca gctcgaggcg
      601 gcccttgcgg gctgccaggc cgagcggccc ccgcccgatg gggggcggcc ctaagggagg
      661 cggcgatgga cgatgaggac cgggccgagc ggctccgccg ccaggccgag gaggccatgg
      721 gcaggaaagg gcccgtctcg aagggggagg aggagctgga cgcgcggcgc cttgtccacg
      781 agctgcgcgt ccaccaggtc gagctcgagc tccagaacga ggagctgcgc gagaccaggg
      841 cgagggccga ggagagcctg gagcgcttcc gggagctctt cgagtacgcc ccggtgcctt
      901 atgtcagcct ggaccggaag ggcacggtcc tccaggccaa cctcgccgcc ggctcggccc
      961 tcagggtgcc gcgctcgcgt ctggtcggcc ggcccttcca gcaccgcctc ccgtccgagc
     1021 ggcgggggga gttcctcgag ttcctcggta agaccatcga cgacgggcgt cagcagccct
     1081 tcgagaccac actgagcgac gactgcgacc gcagcctgtt cgcgcgcatc gtgggcctcg
     1141 gctccgaccg caagccaggg gagtgccgcc tggccatcgt cgacctcagc gaccgggagg
     1201 aggcccgcct cgccattgag cgggccaacg ccgacatcaa ggccctaatg caggagctca
     1261 accaccgcgt caagaacaac ttcggcgtgg tgatgagcct tctcggcatg ggcgagatga
     1321 aggtcgagga ccccagggcc aaggagatcc tcctggattc ccgggccagg gtcatggccc
     1381 tgacccagat ctacgaacgg ctccacctcg agaacgaggt gctcgcgatc gagctctcgg
     1441 gctatctgga ggacctggtc tcctccctcc tctccacata cgctgtcgat gcctcccgca
     1501 tccgccttgt caccgacttc gagagcatga ggctggacgc gcgcaaagcc ggcctggtgg
     1561 gcctggtctt cagcgagctc atctccaacg cgacgaagta cgcctacgga gagggcgagg
     1621 cgggggagct tcgggtctcc ctcgcccgcc gggagggcag gatcgagctc gccgtcgccg
     1681 acgacggccg gggcctgccc gaggggacgg acccgtccac cgggctgggc ttcagcatcg
     1741 ccaggtccct cgcctcccag ctcggcggca ggctcagcct cggctccgga gcggggaagg
     1801 gcctggaggt aaggctggac tttcccgcct gacggcggcc gccccagccc gcgcagggtt
     1861 cgaggaagcc ttttcggctt tgacgaccca gggccacagg tatacagtgg gccacgcgtg
     1921 gtgccgccta aagggggcag cgtccgcctg gaggtcatgg catgaagacg atcaggggcg
     1981 agtccaagct ctacctcgag atagacctgt ccaagcgcag cttcggcgtg ttctctccgg
     2041 gcgaggacga gctcggggac tacaacgggg gcaagggcc
//
LOCUS       DASSQV010000270         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1555764, whole genome shotgun sequence.
ACCESSION   DASSQV010000270 DASSQV010000000
VERSION     DASSQV010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555764"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFL04_04585"
     CDS             complement(<1..641)
                     /locus_tag="VFL04_04585"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7839011.1"
                     /translation="MPDFQFKVGPRVWIGNDALLRLPLLASEIAGADRARVMLVADPI
                     LHESKLVDRVRSLLEDRNIQVLLFDEIPDRATSRAADDALRLARGSRSPLIVAIGGIK
                     TLMIARAAAAAAPSTNDIDELLDGAESRARPLPLIEIPTTLRHPFMFDDCILLSDGRD
                     RRVRMARVAGSAPYAVLIDHGLSGGLSAKLTASCVIDGLLGAVEAYVSSRSSF"
     gene            complement(782..1072)
                     /locus_tag="VFL04_04590"
     CDS             complement(782..1072)
                     /locus_tag="VFL04_04590"
                     /inference="COORDINATES: protein motif:HMM:NF016223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HET7839012.1"
                     /translation="MTIDRLNSMDPIRDPKKSAPAGRAERVGSSDSIAISTDASQKAD
                     LFRAIELAKAAPDTRADRVAELKAKLQDPNYMNDAVLSMTADRIMDQLFGEG"
     gene            complement(1123..1803)
                     /gene="rsmI"
                     /locus_tag="VFL04_04595"
     CDS             complement(1123..1803)
                     /gene="rsmI"
                     /locus_tag="VFL04_04595"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET7839013.1"
                     /translation="MGKLLLVATPIGNLGDITMRALEGLRAADAIACEDTRHTLKLLS
                     HFEIRKPLLSFHANDEERGAARVLGLLDQGKTVAYCSDAGTPGLSDPGAGLARLAREG
                     GHEVSPLPGACAFAALVSASGFGGRSFLFDGFPSPKAGKRKTRVAELMSREESFVLYE
                     SPHRIVRLLSDIASIDPERKVCLGREMTKIYEEFLVGPASEVLGKLSEKEEQRGEFSV
                     LVAGSELA"
     gene            1899..2312
                     /locus_tag="VFL04_04600"
     CDS             1899..2312
                     /locus_tag="VFL04_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839014.1"
                     /translation="MATALAILLAAGLLGAAFLFLSSGRRTARARSAQAPQTGPAAVG
                     PGGCALCGSALKGGERLKSDMGPGSGDRMMRIFGCPHCWPGLSEADRVYAARYCPVCR
                     SRIPDEGWAIARYFERPGRRHVHILGCTVCRPHAS"
     gene            2323..>2656
                     /locus_tag="VFL04_04605"
     CDS             2323..>2656
                     /locus_tag="VFL04_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839015.1"
                     /translation="MEGTLVRKYLFLGFFLCLLVLVARLYYPFMTIFVWSGLLYALLS
                     PFHERVLKKWPGGSQSSGGRRAVSIIFALGGILLVVLPFTYLAASLASQVIELNRFVR
                     HAIETNPNY"
BASE COUNT          417 a          893 c          904 g          442 t
ORIGIN      
        1 atgaaggagg agcgcgagga gacataggcc tccaccgccc cgagcaggcc gtcgatgacg
       61 caggaggcgg tcagcttggc cgagaggcct cccgacaggc catggtcgat caggacggcg
      121 tagggggccg aacccgcgac cctcgccatc cgcacccgcc tgtcccggcc gtccgagagg
      181 aggatgcagt cgtcgaacat gaaggggtgg cgaagggtgg tcgggatctc gatcagggga
      241 aggggcctgg cccggctctc ggccccgtcg aggagctcgt cgatgtcatt ggtggagggg
      301 gctgcagcgg cggcggcccg cgctatcatg agggtcttga tgccgccgat ggcgacgatg
      361 aggggggacc gcgagcccct ggcgaggcgg agggcatcgt cggcggcgcg gctcgtcgcc
      421 ctgtcgggta tctcgtcgaa gaggaggacc tggatgttgc ggtcctcgag gaggctcctg
      481 accctgtcga ccagcttcga ctcgtggagg atggggtcgg cgaccagcat gacgcgcgcg
      541 cggtcggcgc ccgcgatctc ggacgccagg aggggaagcc gcaggagggc gtcgtttcct
      601 atccagaccc gcggaccgac cttgaactgg aaatcgggca cactcgcctc tttcccgacc
      661 gcggccttca ggcccgccgt cggaacacaa aaaaaggcgg aagtccgagc tcccgcctca
      721 aaatcgcctg cgatgggggg ccgcggcggc cacgctcagc gagcgtcccc gggtcccgcg
      781 ctcagccctc gccgaaaagc tggtccatga tccgatcggc cgtcatggag aggacggcgt
      841 cgttcatgta gttgggatcc tggagcttgg ccttgagctc ggcgacgcgg tcggccctgg
      901 tgtcgggcgc ggccttggcc agctcgatgg cccggaaaag atcggccttc tgggaggcat
      961 cggtcgaaat ggcgatggag tcgctcgacc cgacccgctc cgccctgccg gcgggggcgg
     1021 atttcttggg atcgcgaata gggtccatag agttcaggcg gtctatcgtc atctcgctcc
     1081 cctgcctttc acctgaatta tcggccgcgg ttcaaaaata gtttacgcca attcactccc
     1141 cgcgacaagc acggagaatt ccccccgctg ctcctccttc tcggagagct tgccgaggac
     1201 ctcgctggcc ggacccacga ggaattcctc gtagatcttg gtcatttccc ttcccagaca
     1261 gactttacgc tccgggtcta tcgaggcgat gtccgaaaga agccggacga tccggtgcgg
     1321 cgactcatag agcacaaagg actcctcccg ggacatgagc tcggccaccc gggttttgcg
     1381 cttgccggcc ttgggcgagg gaaagccgtc aaaaaggaag gacctgcccc caaaaccgct
     1441 cgcgctcacc agggctgcga aggcgcaggc cccgggaagg ggggagacct cgtggccgcc
     1501 ctcgcgggcg agacgggcaa ggccggctcc cgggtccgag aggccggggg tcccggcgtc
     1561 gctgcagtag gccaccgtct tgccctggtc gaggaggccg aggacccgcg ccgccccccg
     1621 ctcctcgtcg ttggcgtgga aggagagaag gggcttccgt atctcgaagt ggctgaggag
     1681 cttgagggtg tgcctggtgt cctcgcaggc gatcgcatcg gcagccctga gcccctcgag
     1741 ggcgcgcatg gtgatgtcgc cgaggttgcc tatcggggtg gccacaagga ggagcttgcc
     1801 catcgcggcc gagtatggtc cgcggatcgc ggaggggtca agtgcggccc cgggaaacgc
     1861 tccttgccgg aaggccgcgg cggtgtatac tcgctcccgt ggcgacagct ctcgcgatcc
     1921 tcctcgcggc cggcctcctt ggcgcggcct tcctcttcct ttcgtccgga cgcaggacgg
     1981 ccagggcgcg ctcggcccag gctccccaga cgggcccggc cgccgtcgga cccgggggct
     2041 gcgccctctg cggttcggcc ctcaagggcg gcgaaaggct caagtccgac atggggccgg
     2101 gaagcgggga caggatgatg aggatcttcg gctgccccca ctgctggccc gggctttccg
     2161 aggcggacag ggtctacgcg gcccggtact gcccggtctg ccgctcgcgc atccccgacg
     2221 agggctgggc gatagcccgg tatttcgagc gccctggcag gcgccacgtg cacatcctgg
     2281 gctgcacagt ctgccggccc cacgcaagct aggagcgaac atatggaagg caccctcgta
     2341 cggaaatacc tcttcctcgg attcttcctc tgcctcctgg tcctcgtggc ccggctctac
     2401 taccccttca tgacgatctt tgtctggtcg ggcctgctgt acgccctcct ctcccccttc
     2461 cacgagcgcg tgctcaagaa gtggccgggc ggctcccaat cctcgggggg acgcagggcg
     2521 gtctcgatca tcttcgccct cggcggcatc ctcctcgtcg tcctgccctt cacctatctc
     2581 gccgccagcc tcgccagcca ggtcatcgag ctcaaccgct tcgtccgcca cgccatcgag
     2641 accaacccga actacc
//
LOCUS       DASSQV010000271         7742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1680129, whole genome shotgun sequence.
ACCESSION   DASSQV010000271 DASSQV010000000
VERSION     DASSQV010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680129"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFL04_04610"
     CDS             complement(<1..515)
                     /locus_tag="VFL04_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7839016.1"
                     /translation="MNALAVELNDILAGSVAERLLSEMGKRMYFPKGILSQGAEADEK
                     AKRFNATIGMAYDGGAPMILDAVRAAMPSLSPTEAVAYAPTGGIPALRKRWRSELDRK
                     NPSLKGKTCSLPVVVPGLTAAVSYLADLFLDPEDSVVIPDLHWPNYRLIVEERKTAKG
                     LTYPVFAPRGAS"
     gene            654..1475
                     /locus_tag="VFL04_04615"
     CDS             654..1475
                     /locus_tag="VFL04_04615"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HET7839017.1"
                     /translation="MKRIIALTVILSLALAGTAFAQIKAKDGVYFAQETGFSASSGWK
                     EAVVVTVAGGKIAKVAWNGVSNLGLADKKTVAAAGGYGMKKASKLGLEWDQEAANVEA
                     YLVKTQDLGFSKFNAKGETDAISGASLTVKNFFDLVNKALASAPVPKGIYAKDGWYFA
                     EQSAFDKQSGWKDNVLVTVVNGSVVDVLWNGVYKDKTKKSKLVEALAGRYGMGKVAKK
                     GEWNVQAAAVQDAILKVQDPAKIALKNDGTTDAISGASIHVAAVSLAVEALKAAR"
     gene            1572..2111
                     /locus_tag="VFL04_04620"
     CDS             1572..2111
                     /locus_tag="VFL04_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET7839018.1"
                     /translation="MIHGFEDKTPRIDESAFVAWNAEVAGDVELGEGVSVWYGAVLRA
                     DVAPIRAGRLSNIQDGSVIHVDKGVPAILGEGVTLGHRVIIHGARIGDWTLIGMGAIV
                     LDRAEIGSESIVGAGALVTQGKKFPPRSLILGSPAKVVRPLTAEEVLNLHDHAAAYSV
                     LAKRTKDGSTALDDSALSG"
     gene            complement(2112..3257)
                     /locus_tag="VFL04_04625"
     CDS             complement(2112..3257)
                     /locus_tag="VFL04_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7839019.1"
                     /translation="MQYRRLGRTGLDVSVLGFGCMRLPTTGSPERIDEEAATRLLHEA
                     IDRGVNYIDTAWFYHASSFGQAGQSEPFVGRALAGARRERVLLATKLPQQIIRTREDM
                     DGFLSEQLARLGTDHIDFYLVHGLDGASWNRMRDLGVREFLDGARARGQIRFPAFSFH
                     GNRADFPRIVDEYEGWAFGQIQYNYMDTDFQAGYSGLRYAAQKGLGVVVMEPLKGGRL
                     AAKLPPGMQAAIDAGEEAWSPAEWALRYVWNEEGVSLLLSGMNEMAQLEENLRVAETA
                     RAGSLGPSHLAVYEAARTAMRSRMKADCSACRYCQPCPQGVEIPELLAALNASSMWET
                     RDPWACGYTRIAGKADLCTECGQCEGICPQGLPIRAHLKETTAAFAK"
     gene            3350..3556
                     /locus_tag="VFL04_04630"
     CDS             3350..3556
                     /locus_tag="VFL04_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839020.1"
                     /translation="MKQYAIKSEASARGGDSSLLELIGETEGGFYVRIVHRHEDWEDV
                     KEDFMSQELFDTCLRTGYIAALSA"
     gene            complement(3598..4026)
                     /gene="atpC"
                     /locus_tag="VFL04_04635"
     CDS             complement(3598..4026)
                     /gene="atpC"
                     /locus_tag="VFL04_04635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET7839021.1"
                     /translation="MATMHCEVLTPYRHFYAGEVESVLFVTHDGEIEILPAHEPMVAS
                     VGIGILRIRAEGIDRRAALSDGFVRVKTGRVDIFVDAAEWPEEVELERAQAALERAEK
                     RLAEDTEAQAWQVAASRRAAARARNRIAVAAWASASPLEA"
     gene            complement(4036..5433)
                     /gene="atpD"
                     /locus_tag="VFL04_04640"
     CDS             complement(4036..5433)
                     /gene="atpD"
                     /locus_tag="VFL04_04640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET7839022.1"
                     /translation="MAECIGTVVRVTGPILDLRFEGGQMPAILDAIEIQRPDGTTVLG
                     ECVLHTGDGVARCVAMEATEGLSRGLKARGTGSPITVPVGTEVLGRMFDMAGRPIDGG
                     PAVSAATQRAPIHREAPSLEDQRTNAEILETGIKVIDLIAPYAKGGKIGLFGGAGVGK
                     TVVIMELIRNIAAEHSGYSVFAGVGERSREGNELWKEMNESGVVKNTALVFGQMNEPP
                     GARMRVGLSGLTMAEHFRDAMGQDVLLFIDNIFRFVQAGSEVSALLGRIPSAVGYQPT
                     LANEIGALEERITSTRKGSITSVQAVYVPADDLTDPAPATTFTHLDATTVLSRRIVEK
                     GIYPSVDPLASSSRILDPRVVGEEHYGIARRVQEILQRYQELQDIIAILGMDELGPED
                     KLTVYRARKIERYFSQPFHVAEHFTGMSGCYVPLAETIRGFKEIVEGKCDSIPEQAFY
                     MAGTIDEVHERAKNL"
     gene            complement(5445..6305)
                     /gene="atpG"
                     /locus_tag="VFL04_04645"
     CDS             complement(5445..6305)
                     /gene="atpG"
                     /locus_tag="VFL04_04645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET7839023.1"
                     /translation="MRDIRNRMRSVRQSLQVTGAMKLISTAKLRKARLRLDETRPYFE
                     AIRDTMHDIIVHAEASGERWFDKRPDKRERKACTLVITADKGLAGGYNHAVIRFAEEA
                     CPPGSILLPIGQVGKRYFLEKDYVILEDFAVSRKEPTVYEAKDVASFAVDQFLSGRID
                     EFRVVFTRMHSTVKLEPEMIGLLPLDKEPILAKSRHELDSEMYSYEPSEDAVFDVLVP
                     QYLKGIIYGALVEAFASEQAARMTAMDSASKNAEEMLGHLQLVYNRARQAAITSEVSE
                     IVAGAAALQD"
     gene            complement(6316..>7742)
                     /gene="atpA"
                     /locus_tag="VFL04_04650"
     CDS             complement(6316..>7742)
                     /gene="atpA"
                     /locus_tag="VFL04_04650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET7839024.1"
                     /translation="TVVQAGDGIARVYGLDSCFYGELLEFESGGVGMAMNLDEDDVGC
                     VLFAKQDAVREGEVVKRTGRIAELPAGPSMLGRVVDPLGVPIDGQGPIEALRTMPVER
                     RGPSVIDRKGVSVPLQTGILALDAMVPIGRGQRELIIGDRQTGKTAIAVDTIINQKGK
                     GVICVYASIGQKASTTAQVVETLRRYGALDYTIIVAATAKTTAALQYLAPYSACSIAE
                     YFMDEGKDVLVVYDDLSKHAVAYRALSLLLRRPPGREAYPGDVFYLHSRLLERAARMA
                     PEKGGGSITALPIVETQGGDISAYIPTNVISITDGQIFLEGELFFSGVRPAVDVGLSV
                     SRVGGAAQSKAIRKIAGRLRLDLAQYRELAVFAQFGSDLDKATQDRLAHGKRLVEVLK
                     QPQYAPMGLGEEICALYAAVNGYLAKIKVEDVRGFLDQMFKALAYRHAELLQRLTATG
                     ELGAEDEKELKEALEEHSSHARSA"
BASE COUNT         1186 a         2519 c         2760 g         1277 t
ORIGIN      
        1 gaggcgccgc ggggggcgaa gaccgggtag gtcaggccct tggccgtctt gcgctcctcg
       61 acgatgaggc ggtagttggg ccagtggagg tcggggatga cgacgctgtc ctcggggtcg
      121 aggaagaggt cggcgagata ggagaccgct gcggtgaggc cggggacgac cacggggagg
      181 gagcaggtct tgcccttgag gctggggttc ttgcggtcga gctcggagcg ccagcgtttg
      241 cggagggcgg ggatgccgcc ggtgggcgca taggcgacgg cctcggtggg gctcaaggag
      301 ggcatcgccg ccctcaccgc gtcgaggatc atgggggcgc cgccgtcgta ggccatgccg
      361 atcgtggcgt tgaagcgctt cgccttctcg tcggcctctg ctccctggga caggatgccc
      421 ttcgggaagt acatgcgctt gcccatctcg gagagcaggc gctcggccac gctgccggcg
      481 aggatgtcgt tgagctcgac tgcgagtgcg ttcatgaaga ggttccccct tgatcgggcc
      541 accctaaacg cctcggcgtg gacatgtcaa ggctgggcct attccctagc ttgacactca
      601 ggcatatcac tgcttttata tccaatactt tcaatgcagc acggggagga ctcatgaaga
      661 ggatcatcgc acttaccgtc atcctttcgc tggccctggc gggcaccgcc ttcgcccaga
      721 tcaaggccaa ggacggcgtc tacttcgccc aggagaccgg cttctccgca tcgagcggat
      781 ggaaggaggc ggtcgtcgtc acggtggcgg gcggcaagat cgcgaaggtc gcatggaacg
      841 gggtgtccaa cctcggcctc gccgacaaga agaccgtcgc cgccgccggc ggctacggca
      901 tgaagaaggc ctccaagctc ggcctcgagt gggaccagga ggccgccaac gtcgaggcct
      961 acctcgtgaa gacccaggac ctgggcttca gcaagttcaa cgccaagggc gagaccgacg
     1021 ccatctccgg cgcctcgctc accgtcaaga acttcttcga cctcgtcaac aaggccctcg
     1081 cctcggcccc cgtgcccaag ggcatctacg ccaaggacgg ctggtatttc gccgagcaga
     1141 gcgccttcga caagcagtcc ggctggaagg acaacgtcct cgtgaccgtc gtcaacggca
     1201 gcgtggtcga cgtgctctgg aacggggtgt acaaggacaa gaccaagaag tccaagctcg
     1261 tcgaggccct cgccggccgc tacgggatgg gcaaggtggc caagaagggc gagtggaacg
     1321 tccaggccgc cgcggtccag gacgcgatcc tcaaggtcca ggatccggcc aagatcgccc
     1381 tcaagaacga cgggacgacc gacgcgatct ccggagccag catccacgtg gcggccgtca
     1441 gcctcgccgt cgaggccctc aaggccgccc gctaggccgc agaaaacgcc cgccggcgct
     1501 tgaccggcga gggagggggc cgtcaaatta agacggcccc atttttatgc ccaggcgctg
     1561 gaggtgtgct catgatacac ggcttcgagg acaagacccc aaggatagac gagtcggcct
     1621 tcgtggcctg gaacgccgag gtcgcgggcg acgtcgagct gggcgagggc gtgtcggtct
     1681 ggtacggggc cgtcctcagg gcagacgtcg cgcccatccg cgccggcagg ctcagcaaca
     1741 tccaggacgg gagcgtgatc cacgtggaca agggagtccc tgccatcctc ggcgagggcg
     1801 tcaccctggg ccaccgggtc atcatccacg gggcccggat cggcgactgg accctgatcg
     1861 ggatgggagc catcgtcctc gacagggccg agatcggcag cgagagcatc gtgggcgcgg
     1921 gggccctcgt gacccaaggc aagaagttcc cgccccgctc cctcatcctc gggagcccgg
     1981 ccaaggtggt ccgccccctc accgccgagg aggtcctgaa cctccacgac cacgcggcgg
     2041 cctattcggt cctcgccaag cggaccaagg acggctcgac agccctcgac gattcggccc
     2101 tctcgggctg atcacttcgc gaaggcggcg gtcgtctcct tgagatgggc gcggatgggc
     2161 aggccctggg ggcagatgcc ctcgcactgg ccgcattccg tgcagagatc ggccttgccg
     2221 gcgatcctcg tgtagccgca ggcccagggg tccctcgtct cccacatcga ggaggcgttc
     2281 agggcggcga gcagctcggg gatctcgacg ccctggggac agggctggca gtacctgcat
     2341 gcgctgcagt cggccttcat cctcgagcgc atcgccgtcc tcgccgcctc gtagacagcc
     2401 aggtgggagg ggccgaggga gcccgctctg gcagtctcgg ccaccctgag gttctcctcg
     2461 agctgggcca tctcgttcat ccccgagagc aggaggctga ccccctcctc gttccagacg
     2521 tagcggaggg cccattcggc ggggctccag gcctcctcgc ccgcatcgat ggccgcctgc
     2581 atcccgggcg ggagcttggc cgcgagcctg ccccccttca ggggctccat cacgacgacg
     2641 ccgaggccct tctgggccgc gtagcgcagc cccgagtagc ccgcctggaa gtcggtgtcc
     2701 atgtagttgt actggatctg gccgaaggcc cagccctcgt actcgtcgac gatccgcggg
     2761 aaatcggccc tgttgccgtg gaagctgaag gcggggaagc ggatctggcc cctggccctc
     2821 gccccgtcga ggaactcgcg cacgccgaga tcgcgcatgc ggttccatga ggcgccgtcg
     2881 aggccgtgga ccagatagaa gtcgatgtgg tcggtgccga gcctcgcgag ctgctccgag
     2941 aggaagccgt ccatgtcctc gcgggtcctg atgatctgct gggggagctt ggtggcgagg
     3001 aggacccgct cccgcctcgc ccccgcgagg gcccgcccca cgaagggctc gctctgtccg
     3061 gcctggccga agctcgaggc gtggtagaac caggcggtgt cgatgtagtt cacgcccctg
     3121 tcgatggcct cgtggaggag gcgggtcgcc gcctcctcgt cgatgcgttc gggcgagccc
     3181 gtggtgggca gacgcatgca gccgaagccc agcacggaga catcgaggcc ggtgcgtcca
     3241 agtctgcggt attgcacagt gttctccttc gcgcggggtc cggcccttcc ccgcttcagg
     3301 ctcggccgga tggttccgat acttggatta agcaaggagt gagaccgaca tgaaacagta
     3361 cgccatcaaa tcggaagcat cggcccgggg cggggacagc tcgttgctcg agctcatcgg
     3421 agagaccgag ggcggcttct acgtccgcat cgtccaccgc cacgaggact gggaggacgt
     3481 caaagaggac ttcatgagcc aggagctctt cgacacctgt ctccgcacgg gctacatcgc
     3541 cgctctttca gcataggcga gcgctctctc tcggcctagg cgagcgcgcg gcgcggccta
     3601 ggcctccaag ggggaggccg aggcccatgc cgcgaccgcg atgcggttgc gggccctggc
     3661 cgcggcgcgg cgcgatgccg cgacctgcca ggcctgggcc tcggtgtcct cggccagacg
     3721 cttctcagcg cgctcgaggg ccgcctgggc gcgttcgagc tcgacctcct cgggccactc
     3781 ggcagcgtcc acgaagatgt ccaccctccc cgtcttgacc cgaacgaagc cgtccgaaag
     3841 ggcggctctt ctgtctatgc cctcggccct gatgcgcagg atcccgatgc cgaccgaggc
     3901 caccatgggc tcgtgggcgg ggaggatctc gatctcgccg tcgtgggtga cgaagaggac
     3961 ggactcgacc tcgccggcgt agaaatgccg gtagggtgtc aggacttcgc agtgcatcgt
     4021 cgccatgcgc gccgcctaga ggttcttggc ccgctcgtgg acctcgtcga tcgtgcccgc
     4081 catgtagaag gcctgctcgg ggatcgagtc gcacttgccc tcgacgatct ccttgaagcc
     4141 cctgatggtc tcggcgaggg ggacgtagca gccgctcatg cccgtgaaat gctcggccac
     4201 atggaagggc tgggagaagt agcgctcgat cttgcgcgcg cggtagacgg tgagcttgtc
     4261 ctcgggcccg agctcgtcca tgccgaggat cgcgatgatg tcctggagct cctggtatcg
     4321 ctggaggatc tcctggaccc gcctcgcgat gccgtagtgc tcctcgccga cgaccctggg
     4381 gtcgaggatg cgcgaggacg aggcgagggg gtcgaccgag ggatagatgc ccttctccac
     4441 gatgcggcgc gagaggacgg tcgtcgcgtc gaggtgggtg aaggtggtcg ccggcgccgg
     4501 gtcggtgagg tcgtcggccg ggacgtagac ggcctggacc gaggtgatcg agcccttgcg
     4561 ggtcgaggtg atgcgctcct cgagggcgcc gatctcgttc gccagggtcg gctggtagcc
     4621 gacggcgctc gggatgcggc caaggagggc cgagacctcg ctgcccgcct ggacgaagcg
     4681 gaagatgttg tcgatgaaca agaggacgtc ctggcccatc gcatcgcgga agtgctcggc
     4741 catcgtgagg ccggacaggc cgacgcgcat gcgcgccccc ggcggctcgt tcatctggcc
     4801 gaagaccagg gcggtgttct tgacgacccc cgactcgttc atctccttcc agagctcgtt
     4861 gccctcgcgg ctgcgctcgc cgacccccgc gaagaccgag tagcccgagt gctcggccgc
     4921 gatgttgcgg atcagctcca tgatgacgac ggtcttgccg accccggcgc cgccgaagag
     4981 gccgatcttg ccgcccttgg cgtagggggc gatgaggtcg atgaccttga tgcccgtctc
     5041 gaggatctcc gcgttcgtgc gctggtcctc gaggctgggg gcctcgcggt ggatgggcgc
     5101 gcgctgggtg gcggcgctca cggcggggcc gccgtcgatg ggcctgccgg ccatgtcgaa
     5161 catgcggccc aggacctcgg tccccaccgg gacggtgatg gggctgccgg tgccgcgggc
     5221 cttgaggccg cgggagaggc cctcggtggc ctccatcgcg acgcagcggg ccacgccgtc
     5281 gccggtgtgg aggacgcact cgccgaggac cgtggtcccg tcggggcgct ggatctctat
     5341 ggcgtcgagg atggcgggca tctgcccgcc ctcgaagcgc aggtcgagga tgggcccggt
     5401 gacccgcacg acggttccta tgcattcagc catgttctac cttcctaatc ctggagcgcg
     5461 gccgctccgg ccacgatctc ggagacttcg ctcgtgatgg ccgcctgacg ggcccggttg
     5521 tagacgagtt gcaggtggcc cagcatctcc tcggcgttct tcgacgccga gtccatcgcg
     5581 gtcattcgcg ccgcctgctc gctcgcgaag gcctcgacca gggcgccgta gatgatgccc
     5641 ttgaggtact ggggcacgag gacgtcgaag accgcgtcct cgctcggttc gtagctgtac
     5701 atctcgctgt ccagctcgtg gcgggacttg gccaggatcg gctccttgtc caggggcaga
     5761 aggccgatca tctcgggctc gagcttcacg gtcgagtgca tccgcgtgaa cacgacgcgg
     5821 aactcgtcga tccgcccgga gaggaactgg tcgactgcga aggaggccac gtccttggcc
     5881 tcgtacaccg tcggctcctt gcgcgagacc gcgaagtcct cgaggatgac gtagtccttc
     5941 tcgaggaagt agcgcttccc cacctggccg atgggcagga gtatggagcc gggggggcag
     6001 gcctcctcgg cgaagcggat gacggcgtgg ttgtagccgc cggccagccc cttgtccgcg
     6061 gtgatgacga gggtgcaggc cttgcgctcg cgcttgtccg gccgtttgtc gaaccaccgc
     6121 tcgcccgagg cctcggcgtg gacgatgatg tcgtgcatcg tgtcgcggat cgcctcgaag
     6181 tagggccgcg tctcgtccag ccggagccgc gccttgcgca gcttcgccgt cgagatgagc
     6241 ttcatggccc cggtcacctg gagggactgg cggaccgagc gcatccggtt gcggatgtcg
     6301 cgcatcgtct ccacgctatg cgctcctcgc gtggctggag tgctcttcca gggcttcctt
     6361 cagctccttc tcgtcctccg ctcccagctc gcccgtggcc gtgaggcgct ggaggagctc
     6421 ggcgtgcctg taggcgaggg ccttgaacat ctggtccagg aagccgcgca cgtcctcgac
     6481 cttgatcttg gcgagatagc cgttgaccgc ggcgtacagg gcgcagatct cctcgccgag
     6541 gcccatgggg gcgtactggg gctgcttgag cacctcgacg aggcgcttgc cgtgggcgag
     6601 cctgtcctgg gtcgccttgt cgaggtcgct gccgaactgg gcgaagaccg cgagctcgcg
     6661 gtactgggcg aggtccagcc tcagcctgcc cgcgatcttg cggatggcct tggactgggc
     6721 ggcgccgccg acccgcgaga ccgagaggcc gacgtcgacc gcggggcgca ctcccgagaa
     6781 gaagagctcg ccctcgagga agatctggcc gtcggtgatg gagatcacgt tggtcgggat
     6841 gtaggccgag atgtcgccgc cctgggtctc gacgatcggc agggccgtga tcgagccgcc
     6901 gcccttctcc ggagccatcc gcgccgcccg ctcgaggagc ctcgagtgga ggtagaagac
     6961 gtcgccgggg taggcctcgc gccccggggg cctgcgcagg agcagggaga gggcgcggta
     7021 ggcgacggcg tgcttggaca ggtcgtcgta gaccacgagg acgtccttgc cctcgtccat
     7081 gaagtactcg gcgatcgagc aggccgagta gggggcgagg tactggaggg ccgccgtggt
     7141 cttggcggtg gcggccacga tgatcgtgta gtcgagggcc ccgtagcggc gcagggtctc
     7201 cacgacctgg gccgtcgtcg aggccttctg cccgatggag gcgtagacgc agatcacgcc
     7261 cttgcccttc tggttgatga tggtgtcgac ggcgatggcg gtcttgccgg tctggcggtc
     7321 gccgatgatg agctcgcgct ggccgcggcc gatggggacc atcgcgtcga gggcgaggat
     7381 gccggtctgg agggggacgc tgacgccctt gcggtcgatg accgaggggc cgcgtcgctc
     7441 gacgggcatg gtgcggaggg cctcgatcgg gccctggccg tcgatcggga cgccgagggg
     7501 gtcgacgacg cggccgagca ttgaggggcc ggcggggagc tcggcgatgc ggccggtgcg
     7561 cttgacgacc tcgccctcgc gcacggcgtc ctgcttcgcg aagagcacgc agccgacgtc
     7621 gtcctcgtcg aggttcatgg ccatgccgac gccgccggac tcgaactcca acagctcgcc
     7681 gtagaaacag gaatcgaggc cgtagacgcg cgcgatgccg tctcccgcct gcacgaccgt
     7741 cc
//
LOCUS       DASSQV010000272         4844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1182402, whole genome shotgun sequence.
ACCESSION   DASSQV010000272 DASSQV010000000
VERSION     DASSQV010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182402"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(478..786)
                     /locus_tag="VFL04_04655"
     CDS             complement(478..786)
                     /locus_tag="VFL04_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7839025.1"
                     /translation="MDLDISIEQDRVKITITGPIDTAGGSELSTKFAELAKEPGLRHA
                     EFDLSDVSSISSAGIGKLLAFYKHFDQAGGSMKITSLSPQLAKQFKEIHLDQIIPIGG
                     "
     gene            complement(810..1586)
                     /locus_tag="VFL04_04660"
     CDS             complement(810..1586)
                     /locus_tag="VFL04_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7839026.1"
                     /translation="MSSFFPDPKGARLHYQYWEDSSKGYEKVALILHGIGYNSGSYAA
                     LVPPLVADRYKVYALDFAGFGGSPGDRGVGGTLAMADAIEALIRRIEAVEGAREISLI
                     AHSMGAIAALLFLKRYPARRTRIAFVAPLLFGVPVDALPEALSEDRAVLDRIAADRSF
                     ASSMPNALAADLDATVKTLLADPSFMEGRKAAFFVGESDSLVPFEKLKQTVDALPLKE
                     RKFVSFPRERHDPLGGQRGEQTIAAIKAWFSETRFEYRAP"
     gene            complement(1669..1977)
                     /locus_tag="VFL04_04665"
     CDS             complement(1669..1977)
                     /locus_tag="VFL04_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7839027.1"
                     /translation="MDIELKQKGEALSIVIDGNIDTDGGHKLGVALQEAMEMDGIKSI
                     TFDLSTVRTITSSGIGKLMNFFKFIDSKKGTMAVKGISDSLYKQFMEIHLDRIFPIGK
                     "
     gene            complement(1992..3068)
                     /locus_tag="VFL04_04670"
     CDS             complement(1992..3068)
                     /locus_tag="VFL04_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839028.1"
                     /translation="MDRIERIATALRSLDLGSGVAIEGRTFVGPEDALARLRELEGIA
                     EGLASRDLFALYDRRILHDGVFEEDIHASGRYYAYLDFELKGATPEERENLLATALED
                     LVTQYGKDAVLGRIPPRVQGRQVTEGLVVRPEVDDYPSYYSKTRMAYAEKMAADELPK
                     AVLAPIRAEQERLLDSFGWTTVRVQDPTGQVVEVCGDAYVVSEAGLAAIGPYLRIWET
                     LVDRGPEAFYDLAVATDGEYAPDPESEVGRYYAYVRATLKGQSSADRREALLAALPQE
                     AVEALGAETVAALFREEVAGDQVTASLTIRPEAEEYPSYFNAKKIEAGERRAMKQILI
                     NVQAEIIAAVERALGSATKYIILG"
     gene            complement(3069..3794)
                     /locus_tag="VFL04_04675"
     CDS             complement(3069..3794)
                     /locus_tag="VFL04_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839029.1"
                     /translation="MDELISNDSWSEDQINGIENRLLQGIMGCRSYEAVEAAITYAAF
                     LNTVGLTPENYPVFLRMLEIENHWVIDALIGDTDPFLLLSPVQPNKFIIGRIFAMMTK
                     WHKGGIYPKNLSVILGVLQSNYSSPRDGYRIYPLSIADLNGLAKHLDKDKGQEDPLNR
                     TILNILDRIGQLEGVGTDVDMEETAIHASGIRNAFFDDRKRMEDVIPPVLLVKASYHE
                     KEVAPRKRVGIKEIEKKEKEKGK"
     gene            complement(3825..4550)
                     /locus_tag="VFL04_04680"
     CDS             complement(3825..4550)
                     /locus_tag="VFL04_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839030.1"
                     /translation="MATTKAEEQARLWTRENIMTVENRILQQVMSSRTPEAVESAIAY
                     TRFLRLSGLTSDNYPLFLKMLEIENHWVLDALIGDEDPFLFLSTIQPNRYVISKCFTL
                     LTRWHPGGIYPKTLSIALGVLQNAFSSPKDGYKIYPLTIADVDNLGKHLEKEKGQNFP
                     LNRCILDILDRIGSLQGLGWDESMEEVARQSIRIRSFFFDTAKKLEDCIPQVLLVRGD
                     YLKDEVTPRTLYVEDIDMALRHD"
     gene            complement(4569..>4844)
                     /locus_tag="VFL04_04685"
     CDS             complement(4569..>4844)
                     /locus_tag="VFL04_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839031.1"
                     /translation="EEKVGPLAFEKTKFDREMEESLAVLKTGHASARKAKAAAKDEAP
                     AKPAEKKAGAKPKAPAAKKAAAPKAEKKSPTKSPAAKPTTAKPRKSK"
BASE COUNT          731 a         1455 c         1691 g          967 t
ORIGIN      
        1 ggagatcgcg gatctcgccg accgcggaat agtgaagccc gtgattgaca gggtgtttcc
       61 ctttgaggag gcgatcgagg ccctgcttta tgccgagcga ggccacagcc ggggtaaggt
      121 cataatcggt gaaacggacc gcccgcaatc ctagggatcg cgggcgttct tcagatcggc
      181 ggatgatcac aaggccgagt cccagcagtt cgcgtgactc cggccagttc agatcccggt
      241 gcagttcgcg aagcgtgact ccggccagtt cagatccggg tgcagttcgc gaagcgtgac
      301 tcctgccagt tcagatcccg gtgcagttcg cgaagcgaac tgcagatcgg aggctgaggc
      361 cgggcgccga tccacttgcg aagcagttcg cgaagcttca tgcccatatg tgagcagaat
      421 ccgcgtccac gatgcgcgga ttctgcaaat cgggggatga tcctgcaaaa tcgaggatca
      481 tcccccgatg gggatgatct gatccaggtg gatctccttg aactgcttgg cgagctgggg
      541 cgagagcgag gtgatcttca tgctcccccc ggcctggtcg aagtgcttgt agaaggccag
      601 gagcttgccg atgccagcgc tcgagatcga ggagacgtcg gagaggtcga actcggcgtg
      661 tctgaggccg ggctccttgg cgagctcggc gaacttggtg ctgagctcgg agccgcccgc
      721 ggtgtcgatg gggccggtta tcgtgatctt cacgcgatcc tgttcgatgg aaatatcgag
      781 gtccatgcca attcctcctt ggggcagcct taaggcgcgc ggtactcgaa tctggtctcg
      841 ctgaaccatg ccttgatggc ggctatcgtc tgctcgccgc gctgtccccc gagggggtcg
      901 tgcctttccc tggggaagga gacgaacttg cgctccttta gcggaagggc atccacggtc
      961 tgcttgagtt tttcaaaggg cacgagggag tccgactccc ccacgaagaa ggccgccttc
     1021 cgcccctcca tgaaggaggg atcggcgagg agggtcttca cggtcgcgtc gaggtcggcc
     1081 gcgagggcgt tgggcatgga cgaggcgaaa gacctgtcgg cggcgatgcg gtcgagcacg
     1141 gccctgtcct cagagagggc ctccggcagg gcgtccaccg gcacgccgaa gaggaggggg
     1201 gccacgaagg cgatcctcgt cctgcgggcg gggtagcgct tgaggaaaag aagggccgcg
     1261 atcgccccca tcgagtgggc aatcaggctg atctccctcg cgccctcgac ggcctcgatc
     1321 ctgcggatga gggcctcgat ggcgtcggcc atcgcgaggg tcccgccgac gccgcggtcg
     1381 cccgggctcc cgccgaagcc ggcgaagtcc agggcgtaga ccttgtagcg gtcggccacg
     1441 aggggcggca ccaaggcggc gtaggatccg gagttgtagc cgatgccgtg gaggatcagg
     1501 gccaccttct cgtagccctt ggaggagtcc tcccagtact ggtagtgcag gcgcgccccc
     1561 ttcgggtcgg ggaagaacga gctcatgtga ttcacgctgt ggcccctttg tcggcccgtc
     1621 ggccgcgcgc gcccgccaga ttcggcgggg gcgcagcggc cggcttcgtc atttgccgat
     1681 cgggaagatc cggtcgaggt ggatctccat gaactgcttg tagagcgagt ccgagatccc
     1741 cttgaccgcc atcgtgccct tcttggagtc gatgaacttg aagaaattca tgagtttccc
     1801 gatgcccgac gaggtgatgg tcctgacggt gctgaggtcg aatgtgatgc tcttgatgcc
     1861 gtccatctcc atcgcctcct ggagggccac acccagcttg tggcccccgt cggtgtcgat
     1921 gttgccgtcg atgacgatcg aaagggcctc gcccttctgc ttcaactcga tatccatggt
     1981 tcctccggaa atcagcccag gataatgtac ttcgtggcgc tgccgagcgc gcgctcgacg
     2041 gcggcgatta tctcggcctg tacgttgatc agtatctgct tcatggcccg tctctcgccg
     2101 gcctcgatct tcttggcgtt gaagtacgag gggtactcct cggcctcggg gcggatcgtg
     2161 agcgaggcgg tcacctggtc gccggccacc tcctcgcgga agagggcggc gaccgtctcg
     2221 gccccgaggg cttcgacggc ctcctggggc agggcggcca ggagcgcctc ccgcctgtcg
     2281 gccgagctct ggcccttgag cgtggcgcgg acgtaggcgt agtagcggcc gacctcgctc
     2341 tcggggtcgg gggcgtactc gccgtcggtc gcgaccgcaa ggtcgtagaa ggcctcggga
     2401 ccgcggtcga cgagggtctc ccagatcctg aggtagggcc ctatcgcggc gaggccggcc
     2461 tcggagacca cgtaggcgtc gccgcagacc tctaccactt ggcccgtggg gtcctggacc
     2521 cgcacggtcg tccagccgaa cgagtccagg aggcgctcct gctcggccct gatgggggcg
     2581 agcacggcct tgggcagctc gtcggcggcc atcttctcgg cgtaggccat gcgcgtcttg
     2641 gaatagtagg aggggtagtc gtcgacctcg ggcctcacca cgagtccttc ggtcacctgg
     2701 cgcccctgga cccgcggcgg tatccggccg aggacagcgt ccttgccgta ctgcgtcacg
     2761 aggtcctcga gggccgtggc gaggaggttc tcgcgctcct cgggcgtggc ccccttgagc
     2821 tcgaagtcaa ggtaggcgta gtagcggccc gaggcgtgga tgtcctcctc gaagacgccg
     2881 tcgtggagga tgcgccgatc gtagagggcg aagaggtcgc gcgaggcgag gccctccgcg
     2941 atcccctcga gctcccggag ccgggcgagg gcgtcctcgg ggccgacgaa tgtccgcccc
     3001 tcgatcgcca cgcccgaccc gaggtcgaga gagcgcagcg cggtcgcaat gcgctcgatg
     3061 cggtccactt acttcccctt ctctttctct ttcttctcga tctctttgat cccgacgcgc
     3121 ttgcgggggg cgacttcctt ctcgtggtag ctcgccttga cgaggaggac cggcggtatg
     3181 acgtcctcca tgcgcttgcg gtcgtcgaag aaggcgttgc ggatcccgga cgcgtggatc
     3241 gcggtctcct ccatgtcgac gtcggtgccg acgccctcga gctggccgat gcggtcaagg
     3301 atgttcagga tcgtcctgtt gaggggatcc tcctggccct tgtccttgtc gaggtgcttc
     3361 gcgaggccgt tgaggtcggc gatcgagagg gggtagatgc ggtagccgtc gcggggcgag
     3421 ctgtagttgg actgcaggac gccgaggatg accgagaggt tcttcgggta gatgccgccc
     3481 ttgtgccact tggtcatcat ggcgaagatg cgcccgatga tgaacttgtt gggctggacg
     3541 ggggagagga ggaggaaggg gtcggtgtcg ccgatgaggg cgtcgatgac ccagtggttc
     3601 tcgatctcga gcatccgcag gaagacgggg tagttctcgg gggtgaggcc gaccgtgttg
     3661 aggaaggcgg cgtaggtgat ggcggcctcg acggcctcat agctgcggca gcccatgatg
     3721 ccctggagga ggcggttctc gataccgttg atctggtcct cggaccagct gtcgttggaa
     3781 atcagttcgt ccatgagttc tccttctttt ttcccgctgc cgcgtcagtc gtggcggagc
     3841 gccatgtcga tgtcctcgac gtagagggtc ctgggggtca cctcgtcctt gaggtagtcg
     3901 ccgcggacca ggaggacctg ggggatgcag tcctcgagct tcttcgcggt gtcgaagaag
     3961 aagctgcgga tgcggatcga ctgcctggcc acctcttcca tggactcgtc ccagccgagg
     4021 ccctggaggg acccgatccg gtcgaggatg tcgaggatgc agcggttgag ggggaagttc
     4081 tggcccttct ccttctcgag gtgcttgccg aggttgtcga cgtcggcgat cgtgagcggg
     4141 tagatcttgt agccgtcctt gggcgagctg aaggcgttct ggaggacgcc gagggcgatc
     4201 gacagggtct tcgggtagat cccgcccggg tgccagcgcg tcaggagcgt gaagcacttg
     4261 gagatcacgt agcggttggg ctggatcgtg gagaggaaga ggaaggggtc ctcatcgccg
     4321 atcagggcgt ccagcaccca gtggttctcg atctccagca tcttgaggaa gagcggatag
     4381 ttgtccgagg tgaggcccga gagcctcagg aacctcgtgt aggcgatcgc cgactcgacc
     4441 gcctcgggcg tgcgcgaaga catgacctgc tggaggatgc ggttctccac ggtcatgatg
     4501 ttctcgcggg tccagagcct tgcctgctct tcagctttcg tagttgccat tgtccctcct
     4561 tgtctacctt acttgctttt ccgcggcttc gcggtcgtcg gcttcgcagc cgggctcttc
     4621 gtggggctct tcttctcggc cttgggcgcg gcggccttct tggctgccgg cgccttgggc
     4681 ttcgcgcccg ccttcttctc ggccggcttg gcgggggcct cgtccttggc cgccgccttg
     4741 gccttcctcg cgctggcgtg tcccgtcttg aggacggcga ggctctcctc catctctcgg
     4801 tcgaacttgg tcttctcgaa ggcgaggggg ccgaccttct cctc
//
LOCUS       DASSQV010000273         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2427522, whole genome shotgun sequence.
ACCESSION   DASSQV010000273 DASSQV010000000
VERSION     DASSQV010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427522"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFL04_04690"
     CDS             <1..547
                     /locus_tag="VFL04_04690"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HET7839032.1"
                     /translation="AATVLAACLSAGLLGCAKQAEAPFAGPEYLSWRRTVALTLDYPV
                     PGHQAGLRIPRMNSLGFLAEPVKQGDRLRWSFPEGTVIVKEIYATAHPAAGEGPVELT
                     IMVKRPSDPRSRGGWLWLTKNLPGGEERVFLGDYCFTCHENANEAHPYGDHNPNPDFR
                     DYVYFVPGEKVGEAAPTGSAY"
     gene            630..1337
                     /locus_tag="VFL04_04695"
     CDS             630..1337
                     /locus_tag="VFL04_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7839033.1"
                     /translation="MQLSVKDAAAILNVSEKTIYRWIKQEAIPAYRINEQYRFNRAEL
                     LEWATSRRIQVSPDIFLEGDKNALPLPSLSEALKAGGVAYRLGGSDQASVLRAVVDVL
                     HLPEEVDREFLYQVLLARETLGSTGIGDGIAIPHVRNPVVLHVAKPTVTLCFLEHPID
                     FKAIDNKPVDTLFTLISPSVRAHLHLLSRLGFALRDAGFKAAVRRRASREEIMDSLVK
                     AEAALPQAGATPEGAPD"
     gene            1343..>2341
                     /locus_tag="VFL04_04700"
     CDS             1343..>2341
                     /locus_tag="VFL04_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET7839034.1"
                     /translation="MPLQLILAALALSLISGIPSLVLPRTSALGQRCSTALMVLASLL
                     GLAGAAMALAGSPSADLGLPWPALGDSLVGLDPLSAFFLLPVFLIGGLGSVYGLGYWP
                     VYRHRGSGERLQVFWGLMVAGLALLLVARQAMAFLLGWEIMALSAFFLIASEDREAET
                     RRASLVYLMATHVATLALFGLFVLWRHATGSFLLDPVSADRLGLGAMSAIFLLALLVF
                     GLKAGMMPLHFWLPLAHAAAPSQVSAILSGVVLKMGIYGLLRILSLMPNPPTAWGALI
                     LFAGAVSGVVGVAFAIAQHDLKRLLAYHSVENIGIILMGLGLAMLGRSLERPDWVVL"
BASE COUNT          314 a          946 c          689 g          392 t
ORIGIN      
        1 agccgccacc gtcctggccg cctgcctctc ggccggcctc ctcggctgcg ccaaacaggc
       61 cgaggccccc ttcgccgggc ccgagtacct ctcctggcgc cgaaccgtgg cgcttaccct
      121 cgactacccc gtgcccggcc accaggccgg gctcaggatc ccgaggatga actccctggg
      181 cttcctcgcc gagcccgtga agcaggggga caggctccgc tggagcttcc ccgagggcac
      241 ggtgatcgtc aaggagatct acgcgaccgc ccatcccgcg gcgggggagg gccccgtcga
      301 gctgacgatc atggtgaagc ggccctcgga cccccgctcg cgcgggggct ggctctggct
      361 cacgaagaac ctgcccgggg gcgaggagag ggtcttcctc ggggactact gcttcacctg
      421 ccacgagaac gccaacgagg cccatcccta cggggaccac aacccgaacc cggacttcag
      481 ggactatgtc tacttcgtgc ccggcgagaa ggtcggggag gccgcgccca cggggagcgc
      541 ctactaggct tcattgacat tcgaggacaa ttctccctac tatcctctcc tgtcctgtcg
      601 tgggtccaca tacaggcgga gggtcgtaca tgcaactttc ggtcaaggat gccgccgcga
      661 tactcaatgt ctcggagaag accatctacc gctggatcaa gcaggaggcc atccccgcct
      721 accggatcaa cgagcagtac cgcttcaacc gcgccgagct gctcgagtgg gccacctcga
      781 ggcggatcca ggtctcgccc gacatcttcc tcgagggcga caagaacgcc ctgccgctcc
      841 cgagcctctc cgaggccctc aaggccgggg gggtcgccta ccgcctcggg ggctctgacc
      901 aggcctcggt cctccgcgcc gtcgtcgacg tcctccacct gcccgaggag gtcgacaggg
      961 agttcctcta ccaggtcctc ctcgcccgcg agaccctcgg ctcgacgggg atcggcgacg
     1021 ggatcgccat cccccacgtg cgcaaccccg tggtcctcca cgtggccaag cccaccgtga
     1081 ccctctgctt cctcgagcac cccatcgact tcaaggccat cgacaacaag ccggtggaca
     1141 ccctgttcac cctcataagc ccctcggtcc gcgcccacct ccacctcctc tcccggctcg
     1201 gcttcgccct ccgcgacgcc ggattcaagg ccgcggtccg caggcgcgcg tcgagggagg
     1261 agatcatgga cagcctcgtc aaggccgagg ccgccctgcc ccaggccggc gccacgcccg
     1321 agggcgcgcc ggactaagac cgatgccgct ccagctgatc cttgccgcgc tcgccctctc
     1381 cctcatcagc ggcatcccga gcctggtcct gccccgcacc tcggccctgg gccagcgctg
     1441 ctccacggcc ctgatggtcc tcgcctccct cctgggcctg gccggagccg ccatggccct
     1501 cgccggctcc ccatcggccg acctgggcct gccctggccc gccctcggcg actccctggt
     1561 gggcctcgac cccctgagcg ccttcttcct cctgcccgtc ttcctcatcg ggggcctcgg
     1621 ctcggtctat ggcctcggct actggcccgt ctaccgccac cgcggcagcg gcgagcggct
     1681 ccaggtcttc tggggcctca tggtggccgg cctggccctc ctcctcgtgg cgaggcaggc
     1741 catggccttc ctcctcggct gggagatcat ggccctctcg gccttcttcc tcatcgcctc
     1801 cgaggaccgc gaggccgaga cccggagggc gagcctcgtc tacctcatgg ccacccacgt
     1861 cgcgaccctg gcccttttcg gcctctttgt cctgtggcgc cacgcgacgg gctccttcct
     1921 cctcgacccg gtctcggccg acaggctcgg cctcggcgcg atgagcgcca tcttcctcct
     1981 cgccctcctc gtcttcgggc tcaaggccgg catgatgccc ctccacttct ggctccccct
     2041 ggcccacgcc gccgccccga gccaggtctc ggccatcctc tcgggcgtcg tgctcaagat
     2101 gggcatctac ggcctcctgc gcatcctctc cctcatgccc aatcccccga ctgcctgggg
     2161 cgccctcatc ctcttcgcgg gggccgtgag cggcgtcgtc ggcgtggcct tcgccatagc
     2221 ccagcacgac ctcaagcgcc tcctcgccta ccacagcgtg gagaacatcg gcatcatcct
     2281 catgggcctc gggctcgcga tgctcggccg ctcgctcgag cgccccgact gggtggtcct
     2341 c
//
LOCUS       DASSQV010000274         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1058464, whole genome shotgun sequence.
ACCESSION   DASSQV010000274 DASSQV010000000
VERSION     DASSQV010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058464"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFL04_04705"
     CDS             complement(<1..322)
                     /locus_tag="VFL04_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839035.1"
                     /translation="MTRTLAPRRLAFGAFQCAMGLLLLVFTMAPFAWLLISSVSQRRD
                     LITLPYRFEPAHATLERFTAMFSSGGSDIAQAFTYALLNSLIVAGLVTIVGLATGTLA
                     SYSFA"
     gene            complement(315..1244)
                     /locus_tag="VFL04_04710"
     CDS             complement(315..1244)
                     /locus_tag="VFL04_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7839036.1"
                     /translation="MPRWPLKRARAARYRAGDLGEEGFAFLLLSPAGLVVLAVVFLPL
                     AITFAYTLMNVELLSENRGAFVGLGNYASVMGSSWFWQSLGRTMYFTVVSLVLETGLG
                     LLVALLLNGSFPGVSALRALIILPWAIPTNVSAALWKWIYQPEFGALNALLTWLGLLK
                     EYRSWLGDPALAMNMLVLADVWKMMPLAAIFFLAALQFINKALYEAARIDGAGVLRRF
                     WYVTMPHLVPTLLVVVVMRSMEKFKAFDIFYVMTRGGPANGTMVLTYEAYLRGFTNLD
                     YSAAATIAYLIAFIVLGLTVAYLRVLKQEDIND"
     gene            complement(1261..2511)
                     /locus_tag="VFL04_04715"
     CDS             complement(1261..2511)
                     /locus_tag="VFL04_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7839037.1"
                     /translation="MARRSLAVVALVLFALVSAGSLVAADKVVINALFMKQAGYSEED
                     TTAATKDFEAANPNIQVQLTFVPYEELEQKIITSSAAGGYDVVLSDGPFTAKFAKGGI
                     VKTVPDLAAADKADIFPGALDACVYNGKLYGMPWLNDCKYLFYNKTMLEKAGFKAPPA
                     TWDELLKMGKVLKDKGLVQYPIAWSWSQAECLICDYTTIVASFGGAMVDNAGKPTLTS
                     AANKKALQFMVDSIKSGITNPKSTEYIEDNVRDTFDAGNAAFVLNWTYNYAAAKDPSG
                     SKVVNDVVIGTIPGSGSVKSATVNGGQPLAITAGSKHPYEAWTYILYLTGKGFQKKYV
                     QNALPIWKSLYDDPDVVKSNPDVVKAAKVQYAYIVNRPKVPYYSAFSSMLQVKIQEAL
                     LGKKSVDQALKEAQAEAEALAAKK"
     gene            complement(2721..>3206)
                     /locus_tag="VFL04_04720"
     CDS             complement(2721..>3206)
                     /locus_tag="VFL04_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7839038.1"
                     /translation="GLSYAVYSIMGRSASRRGLNPWTSLLYIFAMAAVFLALFSLGSG
                     GSLPGAARRPADFLWLGDSLAGWAVLFLLAAGPTVAGFGLYLVSLSHLPSSVANLVVS
                     LEPAFTMVIAYLLLGERLGALQVLGALAIMGGVLALRLSEAGGGSGPAAEAGRARGQG
                     A"
BASE COUNT          541 a          953 c         1194 g          518 t
ORIGIN      
        1 gggcgaagga gtaggacgcg agggtccccg tggccaggcc gacgatggtg accaggccgg
       61 ccacgatgag gctgttgagg agggcgtagg tgaaggcctg ggcgatgtcg ctgcccccgc
      121 tcgagaacat cgccgtgaag cgctcgagtg tggcgtgggc cggctcgaag cggtaaggca
      181 gggtgatgag gtcccgccgc tgggagacgc tcgatatgag gagccaggcg aagggcgcca
      241 tcgtgaagac gaggaggagg aggcccatcg cgcactggaa ggcgccgaag gccagccttc
      301 ggggggccag ggtcctagtc attgatgtcc tcctgcttca ggacccgaag gtaggcgacg
      361 gtgagcccga gcacgatgaa ggcgatgagg taggcgatgg tcgcggcggc cgagtagtcc
      421 aggttggtga agccgcggag gtaggcctcg taggtgagga ccatggtgcc gttggcgggc
      481 cctcccctcg tcatgacgta gaagatgtcg aaggccttga acttctccat ggagcgcatg
      541 accaccacga cgaggagggt gggcacgagg tggggcatcg tgacgtacca gaagcggcgc
      601 aagaccccgg ccccgtcgat cctcgctgcc tcgtagaggg ccttgttgat gaactggagg
      661 gccgcgagga agaagatggc ggcgaggggc atcatcttcc agacgtcggc gaggacgagc
      721 atgttcatgg cgagggccgg gtcgccgagc cagctgcggt actccttcag gaggcccagc
      781 caggtgagca gggcgttcag ggcgccgaac tcgggctggt agatccactt ccagagggcg
      841 gccgagacgt tggtgggtat ggcccagggc aggatgatga gggccctgag ggccgagacc
      901 ccggggaagg agccgttgag gaggagggcc acgaggagcc cgagcccggt ctcaaggacg
      961 agggagacca ccgtgaagta catcgtccgc ccgagggact gccagaacca ggacgagccc
     1021 atgaccgagg cgtagttgcc gaggcccacg aaggccccgc ggttctccga gaggagctcc
     1081 acgttcatga gggtgtaggc gaaggtgatg gccaggggca ggaagacgac ggcgaggacc
     1141 acgaggccgg ccggcgagag gaggaggaag gcgaagccct cctcccccag gtcgccggcc
     1201 ctgtatcgtg ccgctctcgc tcgtttaagg ggccaacgcg gcatcgttcg tcctgaatcc
     1261 ctatttcttg gccgcgaggg cctcggcctc ggcctgggcc tccttgaggg cctggtcgac
     1321 gctcttcttg ccgagcaggg cctcctggat cttgacctgg agcatcgagg agaaggcgct
     1381 gtagtagggc accttgggcc ggttgacgat gtaggcgtac tggaccttgg cggccttgac
     1441 gacgtcgggg ttggacttca cgacatcggg atcgtcgtag aggctcttcc atatgggcag
     1501 ggcgttctgc acgtacttct tctggaagcc ctttccggtg aggtagagga tgtaggtcca
     1561 ggcctcgtag gggtgcttcg agcccgcggt gatggcgagg ggctggcccc cgttgaccgt
     1621 ggcgctcttc accgagcccg agccggggat cgtgccgatg acgacgtcgt tcacgacctt
     1681 ggagcccgag gggtccttgg ccgcggcgta gttgtaggtc cagttgagga cgaaggcggc
     1741 gttgccggcg tcgaaggtgt cgcggacgtt gtcctcgatg tactcggtcg acttggggtt
     1801 ggtgatcccg ctcttgatcg agtccaccat gaactggagg gcctttttgt tggcggccga
     1861 ggtcagggtg ggcttgccgg cgttgtcgac catcgcgccg ccgaaggagg cgacgatcgt
     1921 cgtgtagtcg cagatgaggc actcggcctg ggaccaggac caggcgatcg ggtactggac
     1981 gaggcccttg tccttgagga ccttgcccat cttgagcagc tcgtcccagg tcgcgggcgg
     2041 ggccttgaag ccggccttct cgagcatcgt cttgttgtag aacaggtact tgcagtcgtt
     2101 gagccagggc atgccgtaga gcttgccgtt gtagacgcag gcgtccaggg ccccggggaa
     2161 gatgtcggcc ttgtccgccg cggcgaggtc gggcaccgtc ttcacgatgc cgcccttcgc
     2221 gaacttggcg gtgaagggcc cgtcggagag gaccacgtcg tagccccccg cggccgagct
     2281 ggtgatgatc ttctgctcga gctcctcgta ggggacgaag gtgagctgga cctggatgtt
     2341 cgggttcgcc gcctcgaaat ccttggtcgc cgccgtcgtg tcctcctcgc tgtagcccgc
     2401 ctgcttcatg aacagggcgt tgatcacgac cttgtccgcc gccacgaggc tcccggcgga
     2461 gaccagggcg aacaggacga gggctaccac cgcgagactt cttcttgcca ttatcggcct
     2521 ccttgcggcc cgccgcgccg cctcctcggg ggcatccccc catgggcgcg gtctgggcct
     2581 agagtcccgg tatgcatcgc ttcgggaatc cggtcgtctc tcccgatagg gagagacgat
     2641 tttcgccccc aaaggtggac gccggggccc ttccggtccg attgtagccc gtcccggcgg
     2701 gaagtgaagc ggccggccgc ctaggctccc tggcccctcg cccgccccgc ctcggccgcc
     2761 ggacctgacc ctcccccggc ctcgctcagc ctgagggcga ggaccccgcc catgatcgcc
     2821 agggcgccga ggacctggag ggcgccgagc cgctcgccga ggaggaggta ggcgatgacc
     2881 atggtgaagg ccggctcgag ggaaacaaca aggttcgcga cactcgaggg cagatggctg
     2941 aggctcacga ggtagaggcc gaagcccgcc accgtggggc cggcggcgag gaggaagagc
     3001 acggcccagc cggcgaggga atcgccgagc cagaggaaat cggccggccg gcgggccgcc
     3061 ccgggcaggg agccgcccga gccaaggctg aagagggcca ggaagacggc ggccatggcg
     3121 aagatgtaca ggaggctcgt ccaggggttg aggccccgcc tcgaggccga gcgccccatg
     3181 atgctgtaga ccgcgtagga gagccc
//
LOCUS       DASSQV010000275         4366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_811636, whole genome shotgun sequence.
ACCESSION   DASSQV010000275 DASSQV010000000
VERSION     DASSQV010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811636"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /gene="tmk"
                     /locus_tag="VFL04_04725"
     CDS             <1..470
                     /gene="tmk"
                     /locus_tag="VFL04_04725"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET7839039.1"
                     /translation="ELGADPGTVAHLFAADRHEHLYGASGIVERLGRGEAVVCDRYIF
                     SSLAYQGVACGPELPLRLNRDFPLPELLLFFDLEPELSMGRIGHRGKREIYEELPFQE
                     KVRKAYHDALRRFESGPMRIVRVDASLPLAEVGDIVLAAVGETLSLPLRGVQA"
     gene            571..1173
                     /locus_tag="VFL04_04730"
     CDS             571..1173
                     /locus_tag="VFL04_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839040.1"
                     /translation="MRHSGLIAAILLILALAGAYAYPVYYKEQYYKLFHLHYIQYPDD
                     TIENIYWLEQAKKADFCNPLYALATIPDPQHWERYRYLLQMHLDLKLIEQHRILGSKF
                     DKRVAYFYNAPWKDQNLESLKTAESAYRAALAYWAETKDLAAKAMKTRFMELEGADFW
                     IDEAARIESGELDYAQIIASDLARLGKVRAAFEKMDAATY"
     gene            complement(1222..2277)
                     /gene="queA"
                     /locus_tag="VFL04_04735"
     CDS             complement(1222..2277)
                     /gene="queA"
                     /locus_tag="VFL04_04735"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET7839041.1"
                     /translation="MRTEDFSFDLPEELIAQFPPERRGESRLLVLDRATGTRTHTAVR
                     DLASLVEPGTLMVFNDSRVRKARVYAEALDTGSKVEFLLLRRLGPAVWEAAAMKLKRQ
                     RPGRRYRFAEGVEAVVVGDWPGLPPEGDTRVLSFDPPIEEDYLERNGHVPLPPYIRRE
                     DAVADAERYQTVFARRTGSAASPTAGLHFTGELLDSLAERGVLTASVTLHVGLGTFIP
                     VRAETLEEHTMHEESYTVPEETAEAVRRAKAEGRPVLAVGTTSLRSLESAWARGRELG
                     MDGLAPGPGSTRIFIYPGYSFRVVDLLFTNFHTPRSTLLMLVSAFAGRERILEAYAEA
                     VAMRYRFFSYGDAMLIK"
     gene            complement(2252..3256)
                     /gene="ruvB"
                     /locus_tag="VFL04_04740"
     CDS             complement(2252..3256)
                     /gene="ruvB"
                     /locus_tag="VFL04_04740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET7839042.1"
                     /translation="MDPGYDELEDERETSLRPRLLGDFVGQDRLKDNLGLFVQAARER
                     SEALDHLFLVGPPGLGKTTLALIAANELGVDFKPTSAPALEKPKDLAGILTTLGERTV
                     FFIDEIHRLKPALEEMLYIAMEDFELDWVIGQGPAARTVRVPLPPFTLVGATTKPGMV
                     SGPLASRFGISLRLGYYEPRELETILRRSASILGVEVDAEAAGLLASSSRGTPRIANR
                     LLRRMRDFAQVEGGGRVSLEIVRKGLERLEIDSLGLERLDRDILRAIVTTYGGGPVGA
                     ETLAISVGEGVDGLEDYYEPYLIQAGLLARTPRGRVATPAAYAHLGLESPREDGRLLF
                     "
     gene            complement(3275..3877)
                     /gene="ruvA"
                     /locus_tag="VFL04_04745"
     CDS             complement(3275..3877)
                     /gene="ruvA"
                     /locus_tag="VFL04_04745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET7839043.1"
                     /translation="MFNSIRGRLSEKRADSICVETGGVEWLLSVPARSVDEFGATGEE
                     VRAYTWLHHYEDGMRLYAFPRSEDREVFLELTKVEGIGPKQALKILSGIRTEELASAL
                     EADDLAALERIPGVGRKTAQKMLLALKGKLHVAEEGPAPGKAKGEHEFSDVVRALADM
                     GFDRKRADEAVKRAALDHPEGSEREREVFRRALVELSAGA"
     gene            complement(4008..>4366)
                     /locus_tag="VFL04_04750"
     CDS             complement(4008..>4366)
                     /locus_tag="VFL04_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET7839044.1"
                     /translation="RLLAIFAGISDLIEEWRPDVAGMESLYFARNVSSAFPVAEAKGV
                     IRLSFARAGVPLEEYPPNALKLAVTGTARAEKAQVQEMLRLVLGLEAIPEPDHAADAL
                     AAAVCRWHHEGPLSRT"
BASE COUNT          671 a         1516 c         1505 g          674 t
ORIGIN      
        1 gcgaactcgg ggctgacccg ggcacggtcg cccacctttt cgccgccgac cgccacgagc
       61 acctctacgg ggcctcgggc atcgtcgagc gcctgggccg gggcgaggcc gtcgtctgcg
      121 accgctacat cttctcgagc ctggcctacc agggcgtcgc ctgcgggccc gagctgcccc
      181 tgaggctcaa ccgcgacttc cccctccccg agctcctcct cttcttcgac ctggagcccg
      241 agctgtccat gggcaggata ggccaccgcg gcaaacggga gatctacgag gagctgccct
      301 tccaggagaa ggtgcggaag gcctaccacg atgccctcag gcgcttcgaa tccggcccga
      361 tgcgcatcgt ccgcgtggac gcgagcctcc ccctcgccga ggtgggcgac atcgtactcg
      421 ccgccgtcgg cgagaccctc tctctccccc tgcgcggggt ccaggcctag acgcacgggg
      481 ggaaacaacg cgggatcgca aaaggtttcc cgccgccagg gccgaatcgc gggtaaacgc
      541 cggccgtctt cgcgccgaca atcagggagt atgcgccaca gcggcctcat cgcagcgatc
      601 ctcctcatcc tcgccctcgc cggggcctat gcctaccccg tctactacaa ggagcagtac
      661 tacaagctct tccacctcca ctacatccag taccccgacg acaccatcga gaacatctac
      721 tggctcgagc aggcgaagaa ggcggatttc tgcaaccccc tctacgccct ggcgacgatc
      781 cccgaccccc agcactggga gcgctaccgc tacctcctcc agatgcacct cgacctgaag
      841 ctcatcgagc agcaccgcat cctcggctcg aagttcgaca aacgcgtagc ctacttctac
      901 aacgccccct ggaaggacca gaacctcgag agcctcaaga ccgccgaatc ggcctaccgg
      961 gcggccctcg cctactgggc cgagaccaag gacctggccg cgaaggccat gaagacaagg
     1021 ttcatggagc tcgagggggc ggatttctgg atcgacgagg ccgcccgcat agaatcgggg
     1081 gagctcgact acgcccagat catcgcttcc gacctcgccc ggctcggcaa ggtgagggcc
     1141 gccttcgaga agatggacgc cgccacctac tgaccgccgg tccttccctc cgggggcgcc
     1201 aacaggcttc gaggcagcgc ttcactttat gagcatcgcg tcgccatagc tgaagaagcg
     1261 gtagcgcatc gcgacggcct cggcgtaggc ctcgaggatc cgctccctgc ccgcgaaggc
     1321 cgacacgagc atgaggaggg tggacctggg cgtgtggaag ttggtgaaga gcaggtccac
     1381 gacccggaag ctgtagccgg gatagatgaa tatccgcgtg ctcccggggc cgggggccag
     1441 gccgtccatg ccaagctccc tcccccgcgc ccaggccgac tcgaggctgc ggaggctcgt
     1501 cgtccccacg gcgaggacgg ggcggccctc ggccttggcc ctgcgcaccg cctccgccgt
     1561 ctcctcgggc acggtgtagg actcctcgtg catcgtgtgc tcctcgaggg tctcggcccg
     1621 caccgggatg aaggtgccga ggccgacgtg gagggtcacg ctcgctgtca ggaccccgcg
     1681 ctccgcgagg gagtccagaa gctccccggt gaaatggagg ccggccgtcg gcgaggccgc
     1741 cgagccggtc cggcgggcga agacggtctg gtagcgctcg gcgtcggcga cggcgtcctc
     1801 cctcctgatg taggggggaa gggggacgtg gccgttccgc tcgagatagt cctcctctat
     1861 cgggggatcg aagctcagca ccctcgtgtc cccctcgggg ggaaggcccg gccaatcgcc
     1921 gacgaccacg gcctcgaccc cctcggcgaa ccggtagcgg cgccccgggc gctggcgttt
     1981 gagcttcatg gccgcggcct cccataccgc cgggccgagc ctccgcagca ggaggaactc
     2041 gaccttggac cccgtgtcaa gggcctcggc gtagaccctg gccttccgaa cgcgggagtc
     2101 gttgaagacc atgagggtcc cgggctcgac cagggatgcc aggtccctaa cggccgtgtg
     2161 cgtcctggtc ccggtggccc ggtcgagcac gaggaggcgg gactcgcccc ggcgctccgg
     2221 ggggaactgg gctatcagct cttcgggaag gtcaaaagag aagtcttccg tcctcacgcg
     2281 gactctccaa tcctagatgc gcataggcgg cgggggtcgc gacccggccg cggggagtgc
     2341 gggcgaggag ccccgcctgg atgaggtagg gctcgtagta gtcctcaagg ccgtcgacgc
     2401 cctcgcccac cgatatggcc agggtctcgg cgcccacggg gcccccgccg taggtcgtca
     2461 cgatggccct gaggatgtcg cggtcgaggc gctcgaggcc gaggctgtcg atctcgagcc
     2521 gctcgagccc cttcctgacg atctcgaggc tcaccctgcc cccgccctcg acctgggcga
     2581 agtcgcgcat cctgcggagc aggcggttgg cgatgcgcgg cgtgccccgg ctcgaggagg
     2641 cgaggaggcc ggcggcctcg gcgtcgacct cgaccccgag gatggaggcg gagcgcctca
     2701 ggatggtctc gagctccctc ggctcgtagt agccgagcct gagcgagatg ccgaagcggc
     2761 tcgcgagggg gcccgacacc atcccgggct tggtcgtggc ccccacgagg gtgaagggag
     2821 gcagggggac ccgcacagtc ctggccgccg ggccctggcc gatgacccag tcgagctcga
     2881 aatcctccat cgctatgtag agcatctcct cgagggcggg tttgagcctg tggatctcgt
     2941 cgatgaagaa gacggtcctc tcgcccaagg tcgtcaggat gccggcgagg tccttgggct
     3001 tctcgagggc gggggcgctc gtgggcttga agtcgacgcc gagctcgttg gcggcgatga
     3061 gggcgagggt cgtctttccg agtcccggcg gccccacgag gaagaggtgg tcgagggcct
     3121 ccgacctctc gcgcgccgcc tggacgaaga ggccgaggtt atccttaagc ctgtcctggc
     3181 cgacgaagtc gccgaggagt cggggccgga ggctggtctc gcgctcgtcc tcgagctcgt
     3241 cgtagccggg gtccatgctc gtcttgcgca ccgcctaggc tcccgccgag agctcgacga
     3301 gggcgcggcg gaagacctcg cgctcccgtt ccgaaccctc ggggtggtcg agggcggcgc
     3361 gcttgaccgc ctcgtcggcg cgcttgcggt cgaagcccat gtcggcgagg gccctgacga
     3421 catcggagaa ctcgtgctcg cccttcgcct tcccaggcgc gggcccctct tccgcgacgt
     3481 ggagcttgcc cttcagggcg agcagcatct tctgggcggt cttccggccg accccgggga
     3541 tgcgctcgag ggcggcgagg tcatctgcct cgagggctga ggcgagctcc tcggtgcgga
     3601 tgcccgagag gatcttgagg gcctgctttg ggcctatccc ctcgactttg gtgagctcga
     3661 ggaagacctc cctgtcctct gaccggggga aggcgtacag ccgcatcccg tcctcgtagt
     3721 ggtggagcca ggtgtaggcc cggacctctt cgccggtcgc cccgaactcg tcgaccgagc
     3781 gggcgggcac tgagagcagc cattcgaccc cgccggtctc gacgcagatc gagtcggcgc
     3841 gtttttcgct gagccggccc ctgatgctgt tgaacatggt cagccgagta tagtccgaag
     3901 ccccggcggg gaacaaggac gggcctcgat cgccggccga gccgggggcc gtctggcgcc
     3961 gcggcaacgc cggtgcccct atgccctgga caggggaccc ggtcccccta agtccttgat
     4021 aggggaccct cgtggtgcca gcggcagacg gcggccgcga gggcgtcggc cgcgtggtcc
     4081 ggctcgggga tggcctccag cccgaggacg aggcggagca tctcctggac ctgggccttt
     4141 tccgccctcg ccgtccccgt gacggcgagc ttgagggcgt tgggcgggta ctcctcgagg
     4201 gggacccccg cgcgggcgaa ggagagccgg atcacgccct tggcctcggc cacggggaag
     4261 gccgagctca cgttccgggc gaagtagagg ctctccatcc cggccacgtc gggcctccat
     4321 tcctcgatga ggtcggatat tcccgcgaag atggcgagga ggcgct
//
LOCUS       DASSQV010000276         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_191298, whole genome shotgun sequence.
ACCESSION   DASSQV010000276 DASSQV010000000
VERSION     DASSQV010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191298"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFL04_04755"
     CDS             <1..731
                     /locus_tag="VFL04_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET7839045.1"
                     /translation="GLLEIDALARIDVSLNGIQIGRRPGRLSRVAFAVDACAESAKRA
                     VEAGAQVLFVHHGLFWGQPYPVVGSLLERLRIFLDADLALYACHLPLDKHPEIGHNAV
                     LARNLGMERREPFGEYHGVDIGFKGRLPSPLTLDEVVKRVLPDGSRPMTLIPGKKAEI
                     ETVAVISGGAAKEASQAIEQGIDLYVTGEPSHNLYHTVVESGMAFLAAGHYATEVWGL
                     KALAAKMAADTDLKTVFIDLPTGL"
     gene            754..1266
                     /gene="lspA"
                     /locus_tag="VFL04_04760"
     CDS             754..1266
                     /gene="lspA"
                     /locus_tag="VFL04_04760"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET7839046.1"
                     /translation="MDSQRKPRAPFFLILIVLLLDQATKALVVAFVHPGTIAFSAFGD
                     FFWIIRQQNLGMAFSLLDRLAPAPRAAILIAVPFILVGAIGVFYFRSRDLTLFQRWAL
                     CGIAGGGAGNLLDRIFRSEGVVDFVSVKFYGLFGLERWPTFNVADSTVVVCAILLAVS
                     TLIQDARKRS"
     gene            1263..1520
                     /locus_tag="VFL04_04765"
     CDS             1263..1520
                     /locus_tag="VFL04_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839047.1"
                     /translation="MTRGLKAVLFVLGATVANIILTGGFFIGLLALYGLTLGRLIKVP
                     TAMPVILGAFVLAVVLASVTYGAVLKRLRKRYDLEKRLGLK"
     gene            1600..>2289
                     /locus_tag="VFL04_04770"
     CDS             1600..>2289
                     /locus_tag="VFL04_04770"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HET7839048.1"
                     /translation="MAGFTGTFEKLGVRVPEVLLPRAGTDFAKWAVVACDQYSSERDY
                     WKRVEDAVAGAPSTLGLIFPEAFLEDADKAERVGRIQATMKDYLARGLVESKGEGFVL
                     IERFTPFEKEPRKGLCIAVDLEKYQYGKDSKSLIRPTEGTIVERLPPRMQIRRGAPLE
                     LPHIMLLVDDPARSIIEPLYARRQTLPRLYDFELMLGSGRIRGWHVKDAALLDQVAQA
                     LERLADPAAYKA"
BASE COUNT          368 a          793 c          719 g          409 t
ORIGIN      
        1 ggggcctcct cgagatcgat gcccttgcca ggatcgatgt atcgctcaac ggcatccaga
       61 tcgggcgcag acctggccgg ctcagccggg tggccttcgc cgtggacgcg tgcgccgaga
      121 gcgcgaagag ggcggtcgag gcgggcgctc aggtcctctt cgtccaccac gggctgtttt
      181 ggggccagcc ctaccctgtg gtcggatccc tgctcgaacg gctgcgcata tttctcgacg
      241 ccgatctcgc gctgtatgcc tgccaccttc ccctggacaa gcacccggaa atcgggcaca
      301 acgcggtgtt ggctcggaat ctcggcatgg agcggcggga gcctttcggc gaatatcacg
      361 gcgtcgatat cggcttcaaa gggcggctgc cctctcccct gacgctggac gaggtggtga
      421 aacgggtgtt gcccgacggc tcgaggccta tgaccctcat acccgggaag aaggccgaga
      481 tagagaccgt ggcggtgatc tcgggcggag cggcgaagga agcgagccag gcaatagagc
      541 agggcatcga cctctacgta acgggagaac cctctcataa cctgtaccac accgtggtcg
      601 aatcgggcat ggccttcttg gcggcgggcc actatgccac cgaggtctgg gggctcaaag
      661 ccctcgccgc caagatggcg gcggataccg atctgaagac ggtattcatc gacctgccga
      721 cgggcctata gtcgagtcac gcgaggaaca accatggatt cgcagcggaa gccacgtgcc
      781 cccttttttc tgatcctcat cgtccttctc ttggatcagg cgaccaaagc ccttgtggtc
      841 gccttcgtgc atcctggcac gatagccttc agcgccttcg gcgatttctt ttggatcatc
      901 cggcagcaga acctgggtat ggcgttcagc ctcctcgatc ggctcgcgcc ggccccgcgg
      961 gcggccatcc tcatcgccgt gcccttcatc ctcgtcggtg cgatcggcgt gttctacttc
     1021 aggagccgtg acctcaccct cttccagcgc tgggccctct gcggcatcgc cggcggcggg
     1081 gcgggcaacc tcctggacag gatcttcagg agcgaggggg tcgtggactt cgtcagcgtc
     1141 aagttctacg ggctcttcgg gctcgagcgc tggcccacct tcaacgtcgc cgactcgacg
     1201 gtcgtggtgt gcgccatcct cctggccgtc tcgaccctca tccaggacgc gaggaagcgg
     1261 tcgtgacgag ggggctcaag gccgtcctct tcgtcctcgg ggcgacggtc gcgaacatca
     1321 tcctcacggg gggattcttc atcggcctcc tcgccctcta cggcctcacc ctcgggaggc
     1381 tgatcaaggt gccgacggcc atgcccgtca ttctgggggc cttcgtcctc gccgtcgtcc
     1441 tcgcctcggt gacctacggg gccgtgctca agcggctccg caagcgctat gacctcgaga
     1501 agcgcctggg cctgaaatag gaagcgtttc tatgcggcgg gctttgaccc gtccgccggc
     1561 tctgctgcat aatcgccgcc gcgcaagaaa ggagcagtaa tggcaggctt tacgggaact
     1621 ttcgaaaaac tgggcgtccg ggtgcccgag gtccttctcc cccgggcggg caccgatttc
     1681 gccaaatggg cggtggtggc ctgcgatcag tacagctccg agcgcgacta ctggaagcgc
     1741 gtcgaggacg cggtcgccgg ggccccctcg accctcggcc tcatcttccc cgaggccttc
     1801 ctcgaggacg ccgacaaggc cgagcgcgtg ggccggatcc aggccacgat gaaggactac
     1861 ctggcccgcg gcctggtcga gtccaagggt gagggcttcg ttctcatcga gcgcttcacc
     1921 cccttcgaga aggagccccg caagggcctg tgcatcgcgg tcgacctcga gaagtaccag
     1981 tacggcaagg actcgaagtc cctcatccgc cccaccgagg gcacgatcgt cgagcgcctc
     2041 cccccgcgca tgcagatccg ccgcggcgcc cccctcgagc tgccccacat catgctcctc
     2101 gtcgacgacc ccgcgcgcag catcatcgag cccctctacg cgcgccgcca gaccctgccc
     2161 cggctctacg acttcgagct catgctcggc tcgggccgga tcaggggctg gcacgtcaag
     2221 gacgccgccc tcctcgacca ggtggcccag gccctcgagc gcctcgccga ccccgcggcc
     2281 tacaaggcc
//
LOCUS       DASSQV010000277         4241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_936466, whole genome shotgun sequence.
ACCESSION   DASSQV010000277 DASSQV010000000
VERSION     DASSQV010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936466"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VFL04_04775"
     CDS             <1..619
                     /locus_tag="VFL04_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839049.1"
                     /translation="TTLLAGADFSYPDGKSYARGTYIYGYYGRLQRRLSPLESHFSGF
                     VFKNSSVEREVDMDGEGRAYARYLTKPLMSYREHLERFAAASDMEILPLRGKGVELRI
                     GRKGRPRAREPGLFAAGPAKGKAEAFLEGYVRSVRALPSPSDPSLRYLGRLKPEERDL
                     WTTLLPAASAFQRANRTRGIGAADLLEMTRSWAIAAVEEALAAGP"
     gene            complement(648..2330)
                     /locus_tag="VFL04_04780"
     CDS             complement(648..2330)
                     /locus_tag="VFL04_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839050.1"
                     /translation="MRPRTSERPGARGRRGLLRVAAIAAGLLVCSCTKAPAVDSFPWT
                     SEAGARLAFHEASGWEPGSEGSFGSDRVSNRYTLDAPLGLKAGQALELGLRLKAGAGP
                     VPPRIRVGLGSPARKAPPIAAAEFPVRDARTDVVLAIDADCQLASVSVEIGGALEKAG
                     LVLERIAIVPAFRGLERIEGGHRVSSDFSMRIEGGRRIIDLTHPFLARPPAAGQRMAI
                     LLGYGAFSGEASAELRGGAKPHRLRLRNSGGRTAFGPEMFGQGLEGLELSLPERVPVD
                     EVFCAAVPEKEAELADLGRVLLSAPAEPGADYELYRWDILPSVVILDFKDYATQDLYL
                     KRLAFFVEKAGFRGRLAPDDEIAPLHGWNAHDYRAEDLAAFFDRARSEAFPLGQTEKG
                     LEALLLDRGIIVSKGARIGPGSGALISISRESAAYQRLTLLVHESTHGIFFTDPAYRD
                     FVKSRWAAVTKDERWFWRLYFDWANYDVGSDYLMANEYQAYLLQQPVARAKDYFTKTL
                     PSRLVEKHPERQAPLDAYMLRYGELFAEGASALEAWLARKYGIGAGRTWFLD"
     gene            complement(2327..3109)
                     /locus_tag="VFL04_04785"
     CDS             complement(2327..3109)
                     /locus_tag="VFL04_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HET7839051.1"
                     /translation="MRRKVERLANDLATVLSSWPMVECVTLGEHSEEDILDPYFALVV
                     DVYHRGSIPPPAERQAAFATAFGAPGAFESALHQPKDRFFVDGLPLRVEYKNAGFIDE
                     FLDRGSDLVWILKNSGTYMFYRIQKGRTLFQRNDWIERVKGRIAEFPNEYWAALRAAF
                     TAKMEHYLSDLGAAAARDDGFFYLESSAGFARSAAAAIFMANRRFEPSHRAFEDQLRH
                     LERLPDDFSGRWETLLRSDLGISRSQKYEVAQLIARSIIKLA"
     gene            3155..3823
                     /locus_tag="VFL04_04790"
     CDS             3155..3823
                     /locus_tag="VFL04_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839052.1"
                     /translation="MALNTLESCRGFRMLALVPGGNVARDLALFRRRIFAEFGEASAL
                     AYPECVPLACGCLGGLPRPASRPSTPALRGRGSGPDPLARAWDGIDGGFELGALARRD
                     ACLCLEVEGPLLELVAASGRFLAGSGIEAMPDPPLALGLGFLLCRFPIPGLEEDEALL
                     RVAALAPPRGRFLDSSLVLYRITRGSAPWSALAWGEEARAHRRSGPRSAAAGPLPSNP
                     AGER"
     gene            3884..>4241
                     /locus_tag="VFL04_04795"
     CDS             3884..>4241
                     /locus_tag="VFL04_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839053.1"
                     /translation="MKSSQAAAIVLALLLCFAATAAATAQDSGSQGPAAPAAPAAPPA
                     PSAPPAGGEAPPPAAPAAPSSGELGDNSPFLAGEQTIGLSAGAAIPVFMAPDSASSER
                     KLLVGGVFSFQYQYFVQ"
BASE COUNT          602 a         1465 c         1514 g          660 t
ORIGIN      
        1 gaccaccctc ctggccggag ccgacttctc ctatcccgac gggaagagct atgcccgcgg
       61 cacctacatc tacgggtact acggcaggct ccagaggagg ctttcccccc tcgagagcca
      121 cttctcgggc ttcgtgttca agaacagctc ggtggagcgc gaggtcgaca tggatggcga
      181 gggccgggcc tacgcccgct acctcaccaa gcccctcatg tcgtacaggg agcacctcga
      241 gcgcttcgcc gccgcctcgg acatggagat cctccccctg cggggcaagg gcgtcgagct
      301 ccggatcgga aggaagggcc ggccgcgggc ccgtgaaccc ggccttttcg cggccggtcc
      361 ggcgaagggc aaggccgagg ccttcctcga gggctatgtc aggtcggtgc gggctctccc
      421 ctcgccgagc gatccctcgc tccgctatct cggccggctg aagcccgagg agcgcgacct
      481 gtggaccacc ctcctgccag cggcgagcgc cttccagcgc gcgaacagga cgagggggat
      541 cggggccgcc gacctcctcg agatgacgag gtcctgggcg atagccgccg ttgaggaggc
      601 cctggcggcc gggccctagc cgccgtcgac cgctggaggc ggccttccta gtcgaggaac
      661 caggtcctgc cggcccctat cccgtacttc ctagcgagcc aggcctcgag ggccgaagcg
      721 ccctcggcga agagctcgcc gtagcggagc atgtaggcat cgaggggagc ctggcgctcg
      781 gggtgcttct cgacaagcct tgagggcagg gtcttcgtga agtagtcctt ggcccgggcg
      841 accggctgct ggagcagata ggcctggtac tcgttggcca tcaggtagtc ggagcccacg
      901 tcgtagttgg cccagtcgaa gtacagcctc cagaaccagc gctcgtcctt cgtgaccgcc
      961 gcccagcggg acttgacgaa gtcgcggtag gcgggatcgg tgaagaagat gccgtgggtc
     1021 gactcgtgga cgaggagggt caggcgctgg taggccgccg actcgcgcga gatcgagatg
     1081 agggcgcccg agccgggtcc gatcctggca cccttgctga cgatgatgcc gcggtccagg
     1141 aggagggcct cgagtccctt ctcggtctga cccaagggga aggcctccga cctggccctg
     1201 tcgaagaagg cggccaggtc ctcggcccgg tagtcgtggg cgttccagcc gtggaggggg
     1261 gcgatctcgt cgtcgggggc gagccggccc ctgaagcccg ccttctcgac gaagaaggcg
     1321 aggcgcttga gatagaggtc ctgggtcgca tagtccttga agtcgaggat gaccacgctg
     1381 ggcaggatgt cccaacggta gagctcatag tcggccccgg gttcggcggg ggccgagagc
     1441 aggacccggc cgaggtcggc gagctcggcc tccttctcgg ggacggccgc gcagaagacc
     1501 tcgtccaccg gcaccctttc gggcagggag agctcgaggc cctcgaggcc ctggccgaac
     1561 atctcgggcc cgaaggccgt cctgccgccc gagttccgca gcctgaggcg gtggggcttg
     1621 gctccgccgc gcaactccgc gctggcctcg cccgagaagg ccccgtagcc gagcaggatg
     1681 gccatcctct gcccagccgc ggggggccgg gcgaggaagg gatgggtcag atcgatgatc
     1741 ctgcggccgc cctcgatccg cattgagaag tccgaggaga ccctgtggcc cccctcgatg
     1801 cgctcgaggc cgcggaaggc ggggacgatg gcgatgcgct ccaggacgag cccggccttc
     1861 tcgagggccc cgccgatctc caccgagacg ctcgccagct gacagtcggc gtcgatggcg
     1921 aggaccacat cggtcctcgc gtcgcggacg gggaactcgg ccgccgcgat cggcggggcc
     1981 ttgcgcgcag ggctgcccag accgacgcgg atcctgggcg ggaccgggcc ggctcccgct
     2041 ttgagccgca gcccgagctc gagggcctgg ccggccttga ggccgagcgg ggcatcgagg
     2101 gtgtagcggt tggagacccg gtccgagccg aaggagccct cgctgcccgg ctcccagccc
     2161 gaggcctcgt ggaaggcgag cctggctccg gcctcggagg tccaggggaa ggagtcgacg
     2221 gcgggggcct tggtgcagga gcagacgagg aggccggccg cgatggccgc cacgcgaagg
     2281 aggcccctgc ggcccctcgc cccgggcctt tcgcttgtcc gaggcctcat gcgagcttga
     2341 tgatgctccg ggcgatcagt tgcgcgacct cgtacttctg cgagcggctt atgccgaggt
     2401 cggagcgcag gagggtctcc cagcggcccg agaagtcgtc gggcaggcgc tccaggtgcc
     2461 tgagctggtc ctcgaaggcc ctgtgggagg gctcgaagcg ccggttggcc atgaagatgg
     2521 cggcggcggc cgagcgggcg aagcccgcgc tcgactcgag gtagaagaag ccgtcgtcgc
     2581 gggccgcggc cgcgccgagg tcggacaggt aatgctccat cttggcggtg aaggcggcgc
     2641 ggagggcggc ccagtactcg ttcgggaact cggcgatgcg ccccttgacc cgctcgatcc
     2701 agtcgttgcg ctggaagagg gtcctgccct tctgtatccg gtagaacatg taggtcccgg
     2761 agttcttgag gatccacacg aggtcggagc cccggtcgag gaactcgtcg atgaagccgg
     2821 cgttcttgta ctccacgcgg aggggaagcc cgtcgacgaa gaagcggtcc ttgggctggt
     2881 gcagggcgct ctcgaaggcg cccggggcgc cgaaggccgt cgcgaaggcc gcctggcgct
     2941 ctgccggcgg agggatggac ccgcggtggt agacatcgac cacgagggcg aagtaggggt
     3001 cgaggatgtc ctcctcggaa tgctcgccga gggtcacgca ctcgaccatt ggccaggacg
     3061 aaaggactgt ggcgagatcg tttgcgagtc gttctacctt ccggcgcatg gggcttcctt
     3121 tacagcttct ttgaccggat tgtatcatgc cggcgtggct ttgaatacat tggaatcctg
     3181 ccgcggtttc cggatgctcg ccctcgtccc gggagggaac gtcgccaggg acctcgccct
     3241 gttcagacgc aggatcttcg ccgagttcgg cgaggcctcg gccctggcct acccagaatg
     3301 cgtccccctg gcctgcggct gcctcggggg cctccctcgg cccgcttcgc gcccctcgac
     3361 tcccgcgctg cgtggccgcg gatcgggccc ggatcctctg gcccgggcct gggacgggat
     3421 cgatggcggc tttgagctcg gcgccctcgc ccggcgcgat gcctgcctct gcttggaggt
     3481 cgagggcccc ctcctcgagc tcgtggccgc gtccgggcgc tttcttgccg gctccgggat
     3541 cgaggccatg cccgatcctc ccctcgccct gggcctcggc ttcctcctct gccgcttccc
     3601 catccccggc ctcgaggagg acgaggccct gctgagggtg gccgccctcg ccccgccccg
     3661 cggccgcttc ctggacagct cgctcgtcct ctatcgcata acgcgcggat cggcgccgtg
     3721 gtcggccctg gcctggggcg aggaggcgcg cgcccatcgc cggtccggcc ctcggtccgc
     3781 cgcggcgggg cctctgccct ccaatcccgc cggggagcgg taaccacgcc gcttctcaag
     3841 ccttttccat cgtgctatat tccctgccac caacggaggc accatgaagt catcgcaggc
     3901 cgcggcaatc gtgctggccc tcttgctctg cttcgccgcg acggccgcgg ccaccgctca
     3961 ggacagcggg agccagggac ccgccgcccc ggcggctccg gctgcccccc cagctccgtc
     4021 ggccccgccg gcggggggcg aggctccccc ccccgcggcg cccgcggccc ccagctcggg
     4081 agagcttggc gacaacagcc ccttcctcgc cggcgagcag accatcggac tctcggccgg
     4141 tgcggccatc cccgtgttca tggcgcccga ctcggcctcg agcgagagga agctcctcgt
     4201 gggaggggtg ttcagcttcc aataccagta cttcgtccag a
//
LOCUS       DASSQV010000278         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1557284, whole genome shotgun sequence.
ACCESSION   DASSQV010000278 DASSQV010000000
VERSION     DASSQV010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557284"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFL04_04800"
     CDS             complement(<1..269)
                     /locus_tag="VFL04_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839054.1"
                     /translation="MKKLFPSALALVLLSLLALSCSSAGASYPKPDAESTKALSAYFS
                     AYNANSSGLDNQTYWTLTSPGHYNIALSSGGVSFSGTVVQSGTTVT"
     gene            complement(389..1576)
                     /locus_tag="VFL04_04805"
     CDS             complement(389..1576)
                     /locus_tag="VFL04_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7839055.1"
                     /translation="MRIARLFILTALAVLVIACQTAKPSPQPAPPPEAPSAPAPSPQA
                     IAPGQSIIQTEVSGFSPTAENGKNSIQFALKFANSDAVKTWKVAIAGKAGVVRSFSGT
                     GPSLPANLSWDGKTDAGAAAAEGSYAASLSIDYGDAFKPSSASSKDFILDGSPPSGKL
                     TVSPGLFSPNEPKAKVTISIEASSTLAKIDSWSMDILDPEGNLFKSFDGKWPDAKVVW
                     DGTGIGGGTVESAEDYPVVVKLRDEFGNVGTLKAGVSVDIVVIKEAKGYRIPNSRIFF
                     KDFTADYHDVPAELQAQNSARLDHLAATLKKFPGYKIRIVGHAVMIHWDSPSLGKIEE
                     ETVLLPLSQARADAIKQAMVDRGLDASLISTEGVGASDQLVPDSDYQNRWRNRRTAIY
                     LEK"
     gene            complement(1800..>2154)
                     /locus_tag="VFL04_04810"
     CDS             complement(1800..>2154)
                     /locus_tag="VFL04_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839056.1"
                     /translation="DYGKTKELAKALLETANKATADSATGLEAAKTESTALAADIAAL
                     VPAVQKQLAEAVKAGKKAKIDVKPIKTGLAAAPKAVADAKAQTEPGLQRQALAALKTS
                     LEGFKAALEAAGFKG"
BASE COUNT          326 a          643 c          762 g          423 t
ORIGIN      
        1 gtaaccgtgg tgccagactg gaccacagtg ccgctgaaac tgaccccgcc ggaactgagg
       61 gcgatgttgt agtgacccgg actggtcagg gtccagtagg tttgattatc cagtccgctg
      121 gagttggcgt tataggccga aaaataggcg gaaagtgcct ttgtagactc ggcgtcgggt
      181 ttaggatagc tcgctccagc cgagctgcag gacagggcga ggagcgacag aaggacgagg
      241 gcaagggcgg atggaaacag ctttttcatg atgcctccgg ggggagtgat gtctattcat
      301 accccccggt tcgccaccag tcaacctgcc ctagttcggg tcgacactcc catacggccc
      361 gccctgagat tgcccgtcga gcctgggctc acttctccag gtagatcgcc gtcctcctgt
      421 tccgccagcg gttctggtag tcggagtcgg ggacgagctg gtcggaggcg cccacgccct
      481 cggtgctgat gaggcttgcg tcgaggcccc ggtcgaccat cgcctgtttg atggcgtcag
      541 ccctggcctg ggagaggggg aggagtacgg tctcctcctc gatcttgccg aggctcgggc
      601 tgtcccagtg gatcatcacg gcgtgcccta cgatcctgat cttgtatccg gggaacttct
      661 tgagcgtggc cgcgagatgg tcgagccggg ccgagttctg ggcctggagt tcggcgggga
      721 cgtcgtggta gtcggcggtg aagtccttga agaagatgcg ggaattgggg atgcggtagc
      781 ccttcgcctc cttgatgacg acgatgtcca ccgagacccc ggccttgagg gtcccgacat
      841 tgccgaactc gtccctgagc ttcacgacga cagggtagtc ctcggccgac tcgacggtcc
      901 ccccgccgat gcccgtgccg tcccagacga ccttggcgtc gggccatttg ccgtcgaagc
      961 ttttgaagag gttcccctcg gggtcgagga tgtccatcga ccaggagtcg atcttggcca
     1021 gggtggagga tgcctcgatg gagatcgtca ccttcgcctt gggctcattc ggggagaaga
     1081 gccccgggct gaccgtcagc ttgcccgagg gcggcgagcc atccaggatg aagtccttgc
     1141 tgctcgccga ggagggcttg aaggcgtcgc cgtagtcgat cgagaggctg gccgcatagc
     1201 tgccctcggc cgccgcggct cccgcgtcgg tcttcccgtc ccaggacagg ttggccggaa
     1261 gggatgggcc ggtgcccgag aagctcctca ccacgccggc cttgccggcg atcgcgacct
     1321 tccaggtttt gacagcatcg ctgttggcga acttcagggc gaactggatc gagttcttgc
     1381 cgttctcggc cgtaggcgag aagcccgaca cctcggtctg gatgatgctc tgtccggggg
     1441 ctatggcctg ggggctcggg gccggggcgc ttggggcttc gggtggcggg gcgggctgcg
     1501 ggctcggctt cgcggtctgg caggcgatga cgaggacggc aagggccgtc aggatgaaca
     1561 ggcgagcgat tctcacggat ctaccctccg acgacccaca cggggatcgt ttcgggcctc
     1621 ggcgcgccgc gcttcctaaa tatcctcgaa tcagcctggg tgcgcccaag cccacctaga
     1681 atatgtatcc ttcgggctga cgctttccaa tctttgaggg gcgtaattga atcgccccag
     1741 tctctgggca aaagaaaggc cgctcccgag ggagcggcca gtgcttggct ccgggcctgt
     1801 tagcccttga aaccggcggc ctcgagggcg gccttgaaac cctcgagcga ggtcttcagg
     1861 gcggcgaggg cctggcgctg gagcccgggc tcggtctggg ccttggcatc ggcgacggcc
     1921 ttgggggcgg cggcgaggcc cgtcttgatg ggcttcacgt cgatcttggc cttcttgccg
     1981 gccttcacgg cctcggcgag ctgcttctgg acggcgggga cgagggcggc gatgtccgcg
     2041 gcgagggcgg tggactcggt cttggcggcc tcaaggccgg tggcggagtc ggccgtcgcc
     2101 ttgttggcgg tctcgaggag ggccttcgcg agctccttgg tcttgccata gtcc
//
LOCUS       DASSQV010000279         5428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_563316, whole genome shotgun sequence.
ACCESSION   DASSQV010000279 DASSQV010000000
VERSION     DASSQV010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563316"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFL04_04815"
     CDS             <1..295
                     /locus_tag="VFL04_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7839057.1"
                     /translation="AKLRGVESRGMLLAASRHGDGGKEAVEVLDAGQASPGSRVILEG
                     QDPAVQAPAEIDADAFFSVPIRAEGGAVRIGSRRLLAGDQSFALARVPDGEVG"
     gene            301..1467
                     /locus_tag="VFL04_04820"
     CDS             301..1467
                     /locus_tag="VFL04_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HET7839058.1"
                     /translation="MRSSAPGGLELAGGDGGEPGKAFLQSAFWGAFKAEFGWKALRFA
                     VRFPAPHNAPGAGGATGPSLLVLVRSLGGGLSFAYLPHGPELAGGLAGDSRLLSSIAE
                     ALRPRLPRGCLFIRFDPPWFVAEAAGEEGEGPASDDAGAFGAHEPLRPAIGLPLVRAA
                     SDVQPPDTVLLDLGPDEDALLAAMKPKWRYNIRLAEKKGVVVREAGPEALGEFYRLYL
                     ATAARDRIALHTERYYARLFSLAEEWAMGRPEAEPKPDLRLWLASHEGESLAGIITIF
                     LGDRAVYLYGASSDEKRNLMPAYALQWAAIKAAKAAGCTEYDFFGIPPSADPGHPMAG
                     LYRFKTGFGGAVVHRPGSWDYALMPFSYRLFRAAEAARAWWFKDFRKRFGRKAG"
     gene            1464..1934
                     /locus_tag="VFL04_04825"
     CDS             1464..1934
                     /locus_tag="VFL04_04825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7839059.1"
                     /translation="MSTASQEEARVRPARAEDAAAISRIVREELGYEASEEEVGAALA
                     LLCPQDSNLILVVERGGRLLGYGHAAAKPSLWLGRIAELQTFAIEAPSQGQGLGSLLL
                     GAIEAELAARGYEQLKLGSRTERAAAHRFYEARGYGREKVSVRFTKRLSPRGSA"
     gene            complement(1912..2298)
                     /locus_tag="VFL04_04830"
     CDS             complement(1912..2298)
                     /locus_tag="VFL04_04830"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HET7839060.1"
                     /translation="MRAEGIRNKRAYEPALPSDGTRILVDRLWPRGLTKAAAAIEIWA
                     KELAPSEGLRRWFGHEGSRFLEFRRLYLDELARLESPLVDRIRELSDVGTVTLVYAAR
                     DPEINHALVLRDFLVGRAGPRPSREG"
     gene            complement(2322..2882)
                     /gene="amrA"
                     /locus_tag="VFL04_04835"
     CDS             complement(2322..2882)
                     /gene="amrA"
                     /locus_tag="VFL04_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HET7839061.1"
                     /translation="MDFDLASGDRELLLAAARESIASRLQGREARRPQTPPALMERCG
                     AFVTLHEAGRLRGCIGRMVGSDPLLETVSSMARAAAFEDPRFPPLSARELGEIDIEIT
                     VLSPLRDCCPDEVEVGRHGIYITKGWHSGVLLPQVATERCWDRETFLEQTCLKAGLPP
                     DAWESPEARVQVFEGIVFGEKGVAGC"
     gene            complement(2883..3734)
                     /gene="amrB"
                     /locus_tag="VFL04_04840"
     CDS             complement(2883..3734)
                     /gene="amrB"
                     /locus_tag="VFL04_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HET7839062.1"
                     /translation="MLEDTGKAKIREATVAGLFYPEGRAELEKEVRSLLAGAHPVASS
                     AAAILAPHAGLAYSGDLAALAWKSALGRRVRHIVILAPLHRSEESLVYLPESELFETP
                     LGAVRVDRELVSDLSDCGTVFQESDIPHFEEHGIEVQLPFMRLLFPEAKLVPVILGKP
                     SPKTVRALAAALGMVFGPNRDECLFVLSSDLAAGAEALGPSTRSSRVLDLIQAGDWQA
                     LLEPGSKDEPGACGGGCIAAYMSSPLAEGTKPVLLGRHDSSACRETREERLVEYAALA
                     FVRPGRP"
     gene            3799..5178
                     /locus_tag="VFL04_04845"
     CDS             3799..5178
                     /locus_tag="VFL04_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7839063.1"
                     /translation="MRALIVDDEKRLGEALAEYLAPEGIEVRTALDGLAGKRLLEEEP
                     FDVLVTDLRMPGLDGLDLLKWAREEGPALPVVVMSAHGEIRDAVSALRLGACDYLVKP
                     FDPDELVMRLHKAVGERRSRDRLEAGRRSYGDGVALVGESQAIQEVLRLIDKAGPASS
                     TVLITGESGTGKEVAARLIHERSGREGPFVAVNLGALPENLVESELFGYEKGAFTGAD
                     ARKVGLFETAHGGTLFLDEIGELALHLQVKILRAIQERKVQRLGALKGVPVDARLVAA
                     TNRDLEAAVRAGSFREDLYYRINVIRIRMPPLRERAGDVPLLAGYFLGKIAAELGRRF
                     DGFSPEALALLERHSFPGNVRELENAIERSLILSEGSVVAARDLQFFAPLPAAGGGGG
                     GARSAPDPLKGGEPMSLAELEHRAIAAALSRNSYHRERTAAELGISRRTLLNKIKEYG
                     LRLPGDPED"
     gene            complement(5188..>5428)
                     /locus_tag="VFL04_04850"
     CDS             complement(5188..>5428)
                     /locus_tag="VFL04_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HET7839064.1"
                     /translation="ELLASCYRESLALAAAEGFASLAFPAISTGVYGYPKDRAALVAL
                     AAIRGFMAASPKPDRVWLVFYTEADALTLLEAAEA"
BASE COUNT          845 a         1877 c         1930 g          776 t
ORIGIN      
        1 cgcgaagctc cgcggggtgg agagccgggg catgctcctc gcggcctccc gacacggcga
       61 tggcggcaag gaggctgtcg aggtcctcga cgccggccag gcaagccccg gcagcagggt
      121 catcctcgag ggccaggatc ccgcagtcca ggctcccgcc gagatcgacg ccgacgcctt
      181 cttctcggtc cccatccgcg ccgagggcgg ggctgtgagg atcggctcca gacgcctcct
      241 cgccggggac cagagtttcg ccctcgcgcg ggtgcccgac ggggaagtag gctgagggcc
      301 ttgcgctcct cggcgccggg cgggctcgag ctggccggag gtgatggcgg ggagccgggc
      361 aaggccttcc tgcagtcggc tttctgggga gccttcaagg ccgagttcgg ctggaaggcc
      421 ctgcgcttcg ccgtcaggtt cccggcgccg cacaacgcgc cgggggccgg gggcgcgacg
      481 gggccgagcc tccttgtcct cgtccggagc ctgggcggag gcctctcctt cgcctacctg
      541 ccccacgggc cggagctggc cgggggcttg gccggcgact cgcgcctcct ctcctccata
      601 gccgaggccc tccgcccgcg tctgccccgc ggctgtctat tcatccgctt cgatccgccc
      661 tggttcgtcg ccgaggcggc aggggaggag ggcgaggggc ccgcctccga cgacgccggg
      721 gccttcgggg cccacgagcc cctgaggccg gcgatcggcc ttcccctcgt cagggccgcc
      781 tccgatgtcc agccccccga cacggtcctc ctcgacctcg gccccgacga ggatgccctc
      841 ctcgcggcga tgaagcccaa atggcgctac aacatccgcc tcgccgagaa gaagggcgtc
      901 gtcgtaaggg aggcggggcc cgaggccctc ggcgagttct accggctcta cctggcgacc
      961 gccgcgaggg accgcatagc cctccacacc gagcgctact acgcaaggct gttctcgctc
     1021 gcggaggaat gggcgatggg aaggccagaa gccgaaccga agcccgacct caggctctgg
     1081 ctcgccagcc acgagggaga gagcctggcc ggcatcatca ccatcttcct cggggacagg
     1141 gcggtctatc tctacggggc ctcctccgac gagaagcgga acctcatgcc cgcctacgcc
     1201 ctccagtggg cggcaatcaa ggcggcgaag gcggccggct gtaccgaata cgacttcttc
     1261 ggcatcccgc ccagtgccga ccctggccat cccatggccg ggctctacag gttcaagacg
     1321 ggtttcgggg gcgccgtggt gcacaggccg ggctcctggg actacgccct tatgcccttc
     1381 tcctataggc tcttccgcgc cgccgaagcg gccagggcct ggtggttcaa ggacttcagg
     1441 aaacgcttcg ggaggaaggc gggatgagca cggcatccca ggaggaggct cgggtgaggc
     1501 cggcgcgggc cgaggacgcg gccgcgattt cgcgcatcgt gcgggaggag ctgggctacg
     1561 aggccagtga ggaggaggtc ggggcggccc tcgccctcct ctgcccccag gactcgaacc
     1621 tcatcctcgt ggtcgagcgg gggggcaggc tgctcggcta cggccatgca gccgccaagc
     1681 cctcgctctg gctcggcagg atagccgagc ttcagacctt cgccatcgag gctccgagcc
     1741 agggccaggg cctcggcagc ctcctcctcg gggcgatcga ggccgagctc gctgcgcggg
     1801 gctacgagca gctcaagctc ggctcgagga ccgagcgcgc ggccgcccac cgcttctacg
     1861 aggccagggg ttacggaagg gagaaggtct cggtccgttt cacgaagcgc ctcagccctc
     1921 gcggctcggc ctaggcccag cccggccgac gaggaagtcc ctgaggacga gggcgtggtt
     1981 gatctcgggg tcccgcgccg catagacgag ggtcaccgtc cccacatccg agagctcgcg
     2041 gatcctatcc acaagggggg attcaagccg ggcgagctca tcgaggtaga gccttcggaa
     2101 ctcgaggaac ctcgagccct catggccgaa ccagcgtcgc aggccctcgc tgggggcgag
     2161 ctccttggcc cagatctcga tggccgcggc ggctttcgtg aggccgcggg gccagagcct
     2221 gtcgacgagg atcctcgtcc cgtccgaagg aagggccggc tcgtaggccc gcttgttccg
     2281 tatgccctcg gcgcgcatct gccacgctcc ccgccgcggg actagcagcc cgcgactccc
     2341 ttctccccga agacgatgcc ctcgaagacc tggaccctcg cctcggggct ctcccaagcg
     2401 tccggcggca gcccggcctt gaggcaggtc tgctcgagga aggtctccct gtcccagcag
     2461 cgctccgtcg ccacctgggg gaggaggacc cccgagtgcc agcccttggt gatgtagatg
     2521 ccatggcggc ccacctcgac ctcgtcgggg cagcagtcgc gcagggggga gaggacggtg
     2581 atctcgatgt cgatctcgcc gagctccctg gccgagagcg gggggaaacg ggggtcctcg
     2641 aaggccgcgg cccgcgccat ggagctgacg gtctcgagga ggggatcgct gcccaccatg
     2701 cgcccgatgc agccgcggag cctccccgcc tcgtggaggg tgacgaaggc cccgcagcgc
     2761 tccatgaggg cgggcggggt ctggggccgg cgggcctcgc ggccctggag ccttgaggcg
     2821 atggactctc gagctgcggc gaggaggagc tcgcggtcgc cggaggccag gtcgaaatcc
     2881 acttagggtc tgcccgggcg gacgaaggcg agggcggcgt attccacgag cctttcctcc
     2941 ctcgtctcgc ggcatgccga ggagtcgtgg cggccaagga ggacgggctt ggtgccttcg
     3001 gccaggggcg acgacatgta ggctgcgatg cagccccccc cgcaggcacc gggctcgtcc
     3061 ttggagcccg gctcgaggag ggcctgccag tccccggcct ggatcaggtc gaggacgcgc
     3121 gaggaccgcg tcgaagggcc aagggcctcg gctccggcgg cgaggtcgga ggagagcaca
     3181 aagaggcatt catcccggtt tgggccgaag accatgccaa gggccgccgc caaggcccgc
     3241 acggtcttgg gactgggctt gccgaggatc acggggacga gcttcgcctc ggggaaaagc
     3301 aggcgcatga agggcagctg gacctcgatg ccatgctcct cgaaatgggg gatgtcgctc
     3361 tcctggaaga cggtgccgca gtcgctcagg tcggacacga gctcgcggtc gaccctgacc
     3421 gccccaaggg gggtctcgaa gagctcggac tccggaagat agacaaggga ctcctctgac
     3481 cggtggaggg gggcgagtat gacgatgtgg cggacgcgtc tgccaagggc cgatttccag
     3541 gccagggccg cgaggtcccc cgaataggcc agacccgcat gcggggctag tatcgccgct
     3601 gccgagctcg ccacgggatg ggctccggcc agaaggcttc ggacctcctt ctcgagctcg
     3661 gccctccctt cgggatagaa gagccccgcg acggtcgctt ctcgaatctt cgcttttcct
     3721 gtatcctcaa gcatacgacc aagtatagac gtgccggctc cacattcaag gacgccgcat
     3781 ggggagggcc tggcagcctt gcgcgcgctc atcgtcgacg acgaaaaacg gctcggtgag
     3841 gccctcgccg aataccttgc ccccgagggc atcgaggtga ggaccgccct ggatgggctc
     3901 gcaggcaagc ggctcctcga ggaggagccc ttcgacgtcc tggtcaccga cctccggatg
     3961 cccggcctcg acggcctcga cctgctcaag tgggcgaggg aggagggacc ggccctcccc
     4021 gtggtcgtga tgtcggccca cggcgagatc cgcgacgcgg tctccgccct gaggctgggg
     4081 gcctgcgact acctcgtgaa gcccttcgac cccgatgagc tcgtcatgcg tctccataag
     4141 gccgtggggg agaggcggtc ccgcgaccgg ctagaggcgg ggaggcggag ctatggggac
     4201 ggcgtcgccc tcgtgggcga gagccaagcc atacaggagg tcctgaggct gatcgacaag
     4261 gccggcccag cctcctccac ggtgctcatc accggtgaga gcggcacggg gaaggaggtc
     4321 gcggcccggc tcatccacga gcgctcgggc cgcgagggcc ccttcgtcgc cgtcaacctc
     4381 ggcgccctgc ccgagaacct cgtggagagc gagcttttcg gctatgagaa gggagccttc
     4441 acgggggccg atgccaggaa ggtgggcctc ttcgagacgg cccacggcgg caccctcttc
     4501 ctcgacgaga tcggggagct cgccctccac ctccaggtca agatcctcag ggccatccag
     4561 gagcgcaagg tccagaggct gggggccctc aagggagtgc cggtcgacgc ccgtcttgtg
     4621 gcggccacaa accgcgacct cgaggccgcc gtcagggcag gcagcttcag ggaagacctc
     4681 tactaccgca taaacgtgat ccggatccgg atgccgcccc tccgcgagcg cgccggcgac
     4741 gtgcccctcc tcgccggcta cttcctcggg aagatcgccg cagagctcgg ccgcaggttc
     4801 gacggcttct cgcccgaggc cctggccctc ctcgagcggc acagcttccc cggcaatgtc
     4861 cgcgagcttg agaacgcgat cgagcgttcg ctcatcctct ccgagggcag cgtcgtggcg
     4921 gcccgcgacc tccagttctt cgcccccctg ccggccgcgg ggggaggggg aggcggggcc
     4981 aggagcgcgc ccgacccgct gaagggcggg gagcccatga gcctcgccga actcgagcac
     5041 cgcgccatcg cggcggccct gtcgcgcaac tcctatcacc gcgaacgcac cgccgccgag
     5101 ctgggcataa gccgcaggac cctcctcaac aagatcaagg agtacggcct caggctgccc
     5161 ggcgatcccg aggactgaaa gagcccctca ggcctcggcg gcctcaagga gggtcagggc
     5221 atcggcctcg gtgtagaaga cgagccagac cctgtcgggc tttgggctcg ctgccatgaa
     5281 gcccctgatc gcggcgaggg ccaccagggc cgccctgtcc ttcgggtagc cgtagacccc
     5341 agtcgagatg gcggggaagg ccagggaggc gaatccctcg gccgcggcga gggccaggga
     5401 ttcccggtag caggatgcga ggagctcg
//
LOCUS       DASSQV010000280         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1059126, whole genome shotgun sequence.
ACCESSION   DASSQV010000280 DASSQV010000000
VERSION     DASSQV010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059126"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFL04_04855"
     CDS             <1..219
                     /locus_tag="VFL04_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839065.1"
                     /translation="QRAMASRLPADRLRVAESGLKDAATLLELYDAGFRAFLVGEHFA
                     TAPEPEAAVRSFVLALAGHAGRSGGGRP"
     gene            216..881
                     /locus_tag="VFL04_04860"
     CDS             216..881
                     /locus_tag="VFL04_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET7839066.1"
                     /translation="MRPRVKICGLTSYEDAALALELGADLIGFVLAPSPRRASPEAVR
                     DIVSRLRAEGRLRQARAVGVFVNEGAEAMAAILAYAGLDEAQVHGDESPEACSLYPFA
                     WYRALRVSGPGDAEGLGRLWAGSPCPRLLFDAASAGAYGGTGLRIGLDAARAAGLACR
                     AASKEFFLAGGLGPDNVAEAIAAIGPDGIDASSALEEGPGRKSALAMRRFFEAVDSAG
                     EAR"
     gene            878..2227
                     /gene="trpB"
                     /locus_tag="VFL04_04865"
     CDS             878..2227
                     /gene="trpB"
                     /locus_tag="VFL04_04865"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7839067.1"
                     /translation="MNRHDERYYGVYGGRYVPEVLRPAFVELEEAWASAQADRGVWTE
                     VERLCGDFIGRPTPLYLAENLSRVLGGPTVYLKLEGLANTGAHKINNALGQALLAKRM
                     GKTRLIAETGAGQHGLATAAAAARFGLECEVFMGELDMERQHPNVFAMRTFGAKVRPV
                     TEGSRTLTDAVNAALKNWTERVSDTHYVLGSALGPHPYPGMVARFQSVIGREARAQIL
                     EREGRLPDAVYACVGGGSNSIGIFQAFLADEVALVGVEAGGRGTGRGMHAVRMDGSAR
                     VGVVQAYKSYFLQDEDGSLLPTHSVSAGLDYAGIGPQLAWLGDSGRLRFIRASDDEAV
                     AALGRTARAEGILPALESAHALAGLFREAPSMDKGSLVIVNVSGRGDKDLFITAPLLD
                     RGDWIAFLERELAALKAGGGGAGPAEGRGAGAAEGQGAGAAEVAGARGPRPLNGEPS"
     gene            2224..3033
                     /gene="trpA"
                     /locus_tag="VFL04_04870"
     CDS             2224..3033
                     /gene="trpA"
                     /locus_tag="VFL04_04870"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET7839068.1"
                     /translation="MSGCGRIEATFASLGGIGLMTHAVCGYPDMEASARILLAASRSG
                     ADFLEAQLPFSEPSADGPLIVGANHEALRRGAGTAAALGMLAALRRTCDTPILVMSYL
                     NPLIARGIDRILGEIGAAGLDGLIVPDYPDDELEYAIAEKCAALGLAFVPLIAPTTSL
                     ERAASLAAASNSPFVYSVLRLGVTGRRTELDATALAGLDALKERTGRRVAAGFGIAER
                     AQVEALSGHADCAVVGSALLSRLMAAIGAGEDPAEAAAGFVASLARSPSGS"
     gene            complement(3011..>3767)
                     /locus_tag="VFL04_04875"
     CDS             complement(3011..>3767)
                     /locus_tag="VFL04_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET7839069.1"
                     /translation="GLVYRVYREAVDLSVPRTVKTLYLFCEALDRAALQPGDLVFFDT
                     DGPLAHVGIYAGEGRFIHAASEGQTRGVMESSLSEPSWTRAYAGAGRLIPPAEYLGLI
                     LTASLGPSLGGGPSMRGASASAGLSSRLLGLEIGLEARPEFDAKLGVLRLPAVLSIAP
                     GKDLRFFAGPALTLGRPTQGSGSGAETYRAEGGLLATAGLVWTPLRFKLAGRDAGIYG
                     QLVYNRYVDETGVAADPGAVLSAGFGISFRWGF"
BASE COUNT          513 a         1362 c         1385 g          507 t
ORIGIN      
        1 cagagggcga tggccagccg tcttcccgct gaccggctcc gcgtggccga gtcggggctg
       61 aaggacgcgg cgaccctgct cgagctctat gacgccggct tccgggcctt cctcgtcggc
      121 gagcacttcg ccacggcccc cgagcccgag gcggcggtgc ggtccttcgt cctggccctg
      181 gccggccacg cggggcgctc cggtggaggc cgaccgtgag gccaagggtc aagatctgcg
      241 ggctcacaag ctacgaggac gcggctttgg ccctcgagct tggagccgac ctcatcggct
      301 ttgtcctcgc gccctcgccc agacgggcct cgcccgaggc ggtgcgggac atcgtctccc
      361 ggctcagggc cgagggcagg ctcaggcagg ccagggccgt cggggtcttt gtgaacgagg
      421 gggcagaggc catggccgcg atcctcgcct acgccggcct tgacgaggcc caggtccatg
      481 gcgacgagag ccccgaggcc tgctccctct accccttcgc ctggtacagg gccctccgcg
      541 tgtcgggacc gggggacgcc gaggggctcg gccgcctctg ggcaggctcg ccctgcccac
      601 gcctcctctt cgacgcggcc tcggccgggg cctacggggg gacggggctc aggatcgggc
      661 tcgatgcggc cagggccgcg ggcctggcct gcagggcggc gtccaaggag ttcttcctgg
      721 ccgggggcct cggccccgac aacgtcgccg aggccatcgc ggccatcggg cccgacggga
      781 tcgacgcctc atcggccctc gaggaggggc ccgggaggaa atcggccctg gcgatgcggc
      841 gcttcttcga ggcggtcgat tccgcggggg aggccaggtg aatcggcacg atgagcgata
      901 ctacggcgtc tacggcggcc gctatgtccc cgaggtcctg aggccggcct tcgtggagct
      961 cgaggaggcc tgggcctcgg cccaggccga ccgcggcgtc tggaccgagg tcgagcggct
     1021 ctgcggcgac ttcatcggga ggcccacccc gctctacctg gccgagaatc tcagccgcgt
     1081 cctcggcggc ccgaccgtct acctcaagct cgagggcctc gccaacacgg gggcgcacaa
     1141 gatcaacaac gccctcggcc aggccctcct cgcgaagcgg atgggcaaga cccggctcat
     1201 agccgagacg ggggccggcc agcacggcct cgcgacggcg gccgccgccg cccgcttcgg
     1261 cctcgagtgc gaggtcttca tgggcgagct ggacatggag cgccagcacc cgaacgtctt
     1321 cgccatgagg accttcgggg cgaaggtgag gcccgtgacc gagggctcgc gcaccctcac
     1381 cgacgctgtc aacgcggcgc tcaagaactg gaccgagcgg gtgtcggaca cccattatgt
     1441 cctcggctcg gccctcggcc cccaccccta cccagggatg gtggcccgct tccagtcggt
     1501 catcggccgg gaggcgaggg cccagatcct cgagcgcgag ggccgactcc ccgacgccgt
     1561 ctacgcctgc gtgggcgggg gctcgaactc catcggcatc ttccaggcct tcctcgccga
     1621 cgaggtggcc ctggtcgggg tcgaggccgg gggccggggg acagggcggg gcatgcacgc
     1681 ggtgaggatg gacggctcgg ccagggtcgg ggtggtccag gcctacaagt cctacttcct
     1741 ccaggacgag gacggctccc ttctgcccac ccattcggtg agcgccggcc tcgactacgc
     1801 cggcatcggg ccccagctcg cctggctcgg cgactcggga cgtctgcgct tcatcagggc
     1861 gagcgacgat gaggcggtgg ccgccctggg ccgcacggcc cgggccgagg gcatcctccc
     1921 cgccctcgag tcggcccacg ccctggccgg ccttttccgc gaggccccct cgatggacaa
     1981 ggggagcctc gtgatcgtga acgtctcggg caggggggac aaggaccttt tcatcaccgc
     2041 ccccctcctc gatcgcgggg actggatcgc cttcctcgag cgcgaactgg ccgcgctcaa
     2101 ggcgggcgga gggggcgcgg gccctgctga gggacggggc gctggcgccg ccgaaggaca
     2161 gggcgcgggc gccgccgagg tggcgggcgc ccggggccct cgccccctga atggggagcc
     2221 atcatgagcg gctgcggacg gatcgaggcg acctttgcct cgctcggcgg catcggcctc
     2281 atgacccacg ccgtctgcgg ctatccggac atggaggcct cggccaggat cctcctcgcg
     2341 gcctcgcgtt cgggggccga cttcctcgag gcccagctgc ccttcagcga gccctcggcc
     2401 gacggccccc tcatcgtcgg cgccaaccac gaagccctgc gccggggggc cggcacagcg
     2461 gccgcgctgg ggatgctcgc ggccctccga aggacctgcg acacccccat cctcgtcatg
     2521 agctacctca atcccctcat cgcgcgcggg atcgacagga tcctggggga gatcggggcg
     2581 gccggcctcg acggcctcat cgtgcccgac taccccgacg acgagctcga gtacgccatc
     2641 gcggagaagt gcgccgccct cggcctggcc ttcgtgcccc tgatcgcccc aaccacgagc
     2701 ctcgagcggg cggcctccct ggccgcggcc tcgaactcgc ccttcgtcta ctcagtcctc
     2761 aggctcgggg tcacggggcg gaggactgaa ctcgacgcca cggccctggc gggcctcgac
     2821 gccctcaagg agaggacggg caggcgggtc gctgccggtt tcggcatcgc cgagcgggcc
     2881 caggtcgagg ccctctcggg ccacgccgac tgcgccgtcg tggggagcgc cctcctctca
     2941 aggctgatgg ccgccatcgg ggccggggag gacccggccg aggccgctgc gggcttcgtg
     3001 gcctccctgg ctagaagccc cagcggaagc tgatcccgaa acccgcgctg agcacggccc
     3061 ccggatcggc ggccactccc gtctcgtcga cgtaacggtt gtacacgagc tggccataga
     3121 tccccgcgtc cctccccgcg agcttgaagc gcaggggggt ccagacgagg ccggcggtcg
     3181 cgaggaggcc gccctcggcc ctgtaggtct cggcccccga gcccgagccc tgggtcggcc
     3241 tgccaagggt cagggccgga ccggcgaaga agcgcaggtc cttgccgggg gctatcgaga
     3301 ggaccgcggg cagcctgagg acgccgagct tcgcgtcgaa ctcgggcctc gcctcgaggc
     3361 cgatctcgag gccgaggagg cgggaggaca ggccggccga ggccgaggcc cccctcatgg
     3421 agggaccgcc gccgagggag ggcccgagcg aggccgtcag gatgaggccg aggtattcgg
     3481 ccggcgggat gaggcggccg gctccggcgt aggccctcgt ccagctgggc tccgagagcg
     3541 aggactccat gacccccctg gtctggccct cggaggcggc gtggatgaag cggccctcgc
     3601 cggcgtagat gccgacgtgg gccaggggcc cgtcggtgtc gaagaagacg aggtctccgg
     3661 gctggagggc ggcccggtcg agggcctcgc agaagaggta gagggtcttg acggtccggg
     3721 gcacgctcag atcgacggcc tcgcggtaaa ccctgtacac gaggccc
//
LOCUS       DASSQV010000281         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1808038, whole genome shotgun sequence.
ACCESSION   DASSQV010000281 DASSQV010000000
VERSION     DASSQV010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808038"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /gene="flgB"
                     /locus_tag="VFL04_04880"
     CDS             complement(<1..281)
                     /gene="flgB"
                     /locus_tag="VFL04_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HET7839070.1"
                     /translation="MFDGTSFGRTVDLLHRSMDVAVLRRNVISDNIANADVPNFKRSV
                     VNFEASLKKALDAEKTRPELELAVTDPKHITNVTRADYRSVEPRRVLDYL"
     gene            complement(571..1992)
                     /gene="hslU"
                     /locus_tag="VFL04_04885"
     CDS             complement(571..1992)
                     /gene="hslU"
                     /locus_tag="VFL04_04885"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HET7839071.1"
                     /translation="MSADLGPADPKGEFAAMTPRRIVEELDRYIVGQDKAKRAVAVAL
                     RNRMRRQRLPADIRDEIAPKNILMIGPTGVGKTEIARRLAKLCGAPFIKVEATKYTEV
                     GYVGRDVESMVRDLMAAGLAMVKAEMQTGVKEEAERRAEERLLDLLLPGIKRADEPIG
                     PSQATFVAPEALAGPAAAHQGGDSREKFRALLRAGKLDSREVEISVQGGGPAIEIFSG
                     NQMEEMEVALGGLSNLFGGKRKKKSVSVVEAKRILAAEELDKLIDPDRAAEEARRRVQ
                     ETGIIFIDEIDKIANREGRGAGGVDVSREGVQRDILPIVEGSTVTTKWGPVDTSHILF
                     IAAGAFNVSKPQDLIPELQGRFPIRVELEALTGKDFERILTEPKNALVVQYRMLLSAE
                     EVELEFTPEAVSRLAEIAAAANALTENIGARRLQTVLERLLEELSFEAPSMAGVKVTI
                     DEAYVDARFKDFAERQDLSRYIL"
     gene            complement(1992..>2512)
                     /gene="hslV"
                     /locus_tag="VFL04_04890"
     CDS             complement(1992..>2512)
                     /gene="hslV"
                     /locus_tag="VFL04_04890"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET7839072.1"
                     /translation="STTIIAVRRDGKVAMAGDGQVTMGATVMKSNARKVRTMNEGKIL
                     VGFAGATADAFTLFEHFEAKIKEYQGDLTRAAVELAKDWRTDKMLRRLEAMLLVADRE
                     KSLLLSGTGDVIEPAEDAVAIGSGGPYAYAAALAYLDGSKLSAREIATKSLGIAGTIC
                     IYTNDRIVVEEI"
BASE COUNT          324 a          873 c          837 g          478 t
ORIGIN      
        1 aggtagtcca ggacccggcg gggctccacg ctgcggtagt cggcccttgt cacgttcgtg
       61 atgtgcttgg ggtcggtgac cgcgagctcg agctcgggcc tcgtcttctc ggcgtcgagg
      121 gcctttttca ggctggcctc gaagttgacg accgagcgct tgaagttggg cacgtcggcg
      181 ttggctatgt tgtcggagat gacgttcctc cgcaggaccg cgacgtccat ggagcggtgg
      241 aggagatcga ccgtcctgcc gaatgaagtt ccgtcgaaca tctagcttct ctccctcggg
      301 gaacctccgc ggacccgcat tctgtccacg cccccgcata cattctcggc cgcccgcccg
      361 gcagcgataa gcgaaacttc ggggccgggc ccgctcggca gtgacccgga accacttttc
      421 ggtgaccccg agccgctcgg tcgcgccccg acctcatccg gccgcgactc gcctcgcttc
      481 cctcacccct ctctcttctc ttttcccctc cgtgccctcc gcggcaaaac gccgcgtctc
      541 cgtggcgtgc ctctctcttc tcttctctcc ctaaaggatg tacctgctca ggtcctggcg
      601 ctcggcgaag tccttgaacc tcgcgtcgac ataggcctcg tctatcgtca ccttaacccc
      661 ggccatggac ggagcctcga aggagagctc ctcgaggagc cgctcgagca cggtctggag
      721 cctgcgggct ccgatgttct cggtgagggc gttggccgcc gcggcgatct cggcgaggcg
      781 tgagactgcc tccggagtga actcgagctc gacctcctcg gccgagagga gcatccggta
      841 ctggaccacg agggcgttct tgggctcggt gaggatgcgc tcgaagtcct tgccggtgag
      901 ggcctcgagc tcgaccctga tcgggaagcg gccctggagc tcggggatga ggtcctgggg
      961 cttcgagacg ttgaaggcgc ccgcggcgat gaagaggatg tgggaggtgt cgacggggcc
     1021 ccacttcgtg gtgacggtcg agccctcgac gatggggagg atgtccctct ggaccccctc
     1081 gcgcgagacg tcgaccccgc ctgcccccct gccctcgcgg ttggctatct tgtcgatctc
     1141 gtcgatgaag atgatcccgg tctcctggac cctgcggcgg gcctcctcgg ccgccctgtc
     1201 ggggtcgatg agcttgtcga gctcctcggc cgccaggatc cgcttggcct cgaccacgct
     1261 caccgacttc ttcttccgct tgccgccgaa gaggttcgag agcccgccga gcgcgacctc
     1321 catctcctcc atctggttgc cggagaagat ctcgatcgcc ggccccccgc cctggacgct
     1381 gatctccacc tcgcgggagt cgagcttgcc cgcccggagc agggcgcgga acttctcgcg
     1441 tgagtccccg ccctgatggg ccgcggccgg ccccgcgagg gcctcgggcg ccacgaaggt
     1501 ggcctgggag gggccgatgg gctcgtcggc ccgcttgatc ccgggcagga ggaggtcgag
     1561 gaggcgctcc tcggccctcc gctcggcctc ctcctttacc cccgtctgca tctcggcctt
     1621 gaccatggcg aggccggcgg ccatgaggtc gcgcaccatg gactcgacgt cgcggcccac
     1681 gtagcccacc tcggtgtact tggtggcctc gaccttgatg aagggggccc cgcagagctt
     1741 ggcgaggcgg cgggctatct cggtcttgcc cacgcccgtg ggcccgatca tgaggatgtt
     1801 cttgggcgcg atctcgtcgc ggatgtcggc ggggaggcgc tggcggcgca tgcggttgcg
     1861 cagggccacg gcgacggcgc gcttggcctt gtcctggccc acgatgtagc ggtcgagctc
     1921 ctcgacgatc ctgcgcggcg tcatggccgc gaactcgccc ttgggatcgg cgggcccgag
     1981 atcggcgctc atcagatctc ctccacgacg atcctgtcgt tggtgtagat gcatatggtc
     2041 cccgcgatgc cgaggctctt ggtggcgatc tccctcgccg agagcttgct gccgtcgagg
     2101 taggccaggg ccgccgcgta ggcgtagggc ccccccgagc cgatggccac ggcgtcctcg
     2161 gcgggctcga tcacgtcgcc ggtgcccgag aggaggaggg acttctcgcg gtcggccacg
     2221 aggagcatgg cctcgaggcg cctgagcatc ttgtcggtcc tccagtcctt ggcgagctcg
     2281 acggcggccc gggtgaggtc gccctggtac tccttgatct tggcctcgaa gtgctcgaag
     2341 agggtgaagg cgtcggcggt cgcgccggcg aaacccacga ggatcttgcc ctcgttcatc
     2401 gtccgcacct tgcgcgcgtt gctcttcatg accgtggcgc ccatggtcac ctggccgtcc
     2461 ccggccatcg ccaccttccc gtcccgcctc acggctatga tcgtcgtcga tc
//
LOCUS       DASSQV010000282         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1060269, whole genome shotgun sequence.
ACCESSION   DASSQV010000282 DASSQV010000000
VERSION     DASSQV010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060269"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2242)
                     /locus_tag="VFL04_04895"
     CDS             complement(<1..>2242)
                     /locus_tag="VFL04_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET7839073.1"
                     /translation="ITSLTIRLRKAEADRVFAVEALTIGKVQEILVQVQRQGLPLWHV
                     GFFNPTAARLKNMVPAKTHHGEPHPRPVLPESYIMMLACPESKAAELEPRLKSIVAAA
                     GASWLPDWMRDHEWEDRFNPMKAKRLGPSIVPAEVIIPLSSLAAFFNRLDASLKPPVV
                     VEATAVKGEEVILLCLIPHDRRKLSFNLAFTLSLSILRLAKRLGGRAYAAGLFLAQEA
                     RAVHGMKLKLWEDHKRNKDDRDLFNSRKLSGIWLMKTMITLALWFEPIVRIGGNLVKP
                     RLKEVWKARRGVPGDVLWYAYACAGCGYCRQVCTLYDGKGWESSSPRGKWAFIRRVAE
                     GKDSFDQEATDSFLMCTTCERCDFECQLDLPIEPSWGVMRGELVNTGRNMSFPAFELM
                     AASARKEKNIWASFAADRDKWIPEDLRPRIKDKAEVAYFAGCTAAFVEQDIAVGSARL
                     LDAAGIEFTTLGKDEACCGIPMLVAGRWDVFEEILRHNIAKMKEKGVKTVVASCPACW
                     LSWNTYYPQWAKKLGIEYGIEAKHYSELLAEKIDSGELKFTHRVDTKVTFHDSCHIGR
                     AGGVYEPPRKLIQAIPGVELVEMAHNRENGLCCGSVLTRISEPEPTSNVLGKRKIDEA
                     IATGAEAVVALCPCCQFQMRVASDANGQGMPVKDLASLAAKGLGIELPDPTAYALEMW
                     GVFDKVITLMKTENMAKLMTALIPSMIRALPLPLRGMMQLAKLPLLDRLMAAMMPGMM
                     PLLMPIL"
BASE COUNT          337 a          681 c          828 g          396 t
ORIGIN      
        1 gaggatcggc atgagcagcg gcatcatccc gggcatcatc gcggccatga gcctgtcgag
       61 gagggggagc ttggccagct gcatcatgcc gcgcagcggc aggggcaggg ccctgatcat
      121 gctggggatg agggcggtca tgagcttggc catgttctcg gtcttcatga gggtgatgac
      181 cttgtcgaag acgccccaca tctcgagggc gtaggcggtc gggtcgggca gctcgatgcc
      241 gaggcccttg gccgcgaggg aggcgaggtc cttgacgggc atgccctggc cgttggcgtc
      301 cgaggcgacc cgcatctgga actggcagca ggggcagagg gccacgacgg cctcggcgcc
      361 cgtggcgatg gcctcgtcga tcttgcgctt tccgaggacg ttggaggtcg gctcgggctc
      421 gctgatcctc gtgaggaccg agccgcagca gaggccgttc tcgcggttgt gcgccatctc
      481 gaccagctcg accccgggga tggcctggat gagcttgcgc ggcggctcgt agaccccgcc
      541 ggcccggccg atgtggcagg agtcgtggaa ggtgaccttg gtgtcgaccc tgtgggtgaa
      601 cttgagctcg cccgagtcga tcttctcggc caggagctcg gagtagtgct tggcctcgat
      661 gccgtactcg atgccgagct tcttggccca ctggggatag taggtgttcc aggagagcca
      721 gcaggcgggg caggaggcga cgacggtctt gacccccttc tccttcatct tggcgatgtt
      781 gtgcctgagg atctcctcga agacgtccca gcgcccggcg acgagcatgg ggatgccgca
      841 gcaggcctcg tccttgccca gggtggtgaa ctcgatcccc gcagcgtcga ggaggcgggc
      901 cgagcccacc gcgatgtcct gctcgacgaa ggcagcggtg caacccgcga aataggccac
      961 ctcggccttg tccttgatcc tcggcctcag gtcctcgggg atccacttgt cacggtcggc
     1021 ggcgaagctc gcccagatat tcttctcctt gcgggcgctc gcggccatga gctcgaaggc
     1081 cgggaagctc atgttcctgc cggtgttgac gagctccccg cgcatgacgc cccacgaggg
     1141 ctcgatgggc aggtcgagct ggcactcgaa gtcgcagcgc tcgcaggtgg tgcacatgag
     1201 gaaggagtcg gtggcctcct ggtcgaagct gtccttgccc tcggccaccc tgcggatgaa
     1261 ggcccacttg ccgcgggggc tcgaggattc ccagcccttg ccgtcgtaga gggtgcagac
     1321 ctggcggcag tagccgcagc ccgcacaggc gtaggcgtac cagaggacgt cgccggggac
     1381 tccccgcctg gccttccaca cttccttgag cctcggcttg accaggttgc cgccaatccg
     1441 cacgatcggc tcgaaccaga gggcgagggt gatcatggtc ttcatgagcc agatgcccga
     1501 gagcttgcgg gagttgaaga ggtccctgtc gtccttgttg cgtttgtggt cctcccacag
     1561 cttcagcttc atgccgtgga ctgcccgcgc ctcctgggcg aggaagaggc cggccgcgta
     1621 ggcccggccg ccgaggcgct tggccaggcg gaggatgctg agcgagaggg tgaaggcgag
     1681 gttgaagctg agcttcctgc ggtcgtgggg gatgaggcag aggaggatca cctcctcgcc
     1741 cttgaccgcc gtggcctcca ccacgacggg gggcttgagg ctcgcgtcga gccggttgaa
     1801 gaaggcggcg agggaggaga gggggatgat gacctcggcg ggcacgatgg aggggccgag
     1861 gcgcttggcc ttcatggggt tgaagcggtc ctcccactcg tggtcgcgca tccagtcggg
     1921 gagccaggag gcccccgcgg cggcgacgat ggacttgagc ctgggctcga gctcggcggc
     1981 cttggattcg gggcaggcca gcatcatgat gtaggactcg gggaggacgg gccggggatg
     2041 gggctcgccg tggtgggtct tcgccggcac catgttcttg agccgggccg ccgtggggtt
     2101 gaagaagccg acgtgccaga ggggcaggcc ctgtctctgg acctggacca ggatctcctg
     2161 gaccttgccg atcgtgaggg cctccaccgc gaagacccgg tccgcctcgg ccttcctgag
     2221 cctgatggtg aggctcgtga tg
//
LOCUS       DASSQV010000283         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_564617, whole genome shotgun sequence.
ACCESSION   DASSQV010000283 DASSQV010000000
VERSION     DASSQV010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564617"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFL04_04900"
     CDS             complement(<1..382)
                     /locus_tag="VFL04_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET7839074.1"
                     /translation="MFELPEYLVLASQIRSELLGKKLRRASTKNVEHRFVWHNLEPGD
                     FAAAAQGRTIGQPRVRGKWMSVPLEPGYVLLIGECGGDLRYEASAASAPRSYHLLIEF
                     EDGSALWARTGMWGAYELYKAGEEL"
     gene            492..1103
                     /locus_tag="VFL04_04905"
     CDS             492..1103
                     /locus_tag="VFL04_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HET7839075.1"
                     /translation="MEPSFISATVLLIVITDPLGNITPVLAALRGVEPPRRKVIILRE
                     CAIAFFVLLLFMFFGQQFLSVLQLKEETLKVAGGVILFLIAINMVFPGSGAKIAGAEG
                     GREPFIVPIAIPLISGPSAMVTVMIISGSNSHRMLEWVFALLITIAVSAVVMLLSTRL
                     QRILGDQVITAFERLMGLVLTAISIDMLLSGVAAYIRILQLGS"
     gene            complement(1215..>2274)
                     /locus_tag="VFL04_04910"
     CDS             complement(1215..>2274)
                     /locus_tag="VFL04_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7839076.1"
                     /translation="AAWSNEYAAWKKDEPALAAELESYLSGKPRKDLDLPSFAKGEKI
                     ATRSASGKALGAVAKAWTNLVGGSADLTGPNVTQLPEGGIYTASTPAGRMIYFGVREH
                     AMAAIANGISLHGGLRPFVATFLTFTDYLKPALRLSALMRQPVVYVMTHDSIYLGEDG
                     PTHQPIEHLAALRAIPNLRVLRPGDAEETAVAWRLAMERREGPTVIALSRQNLPVVEK
                     DDPEWPYTMGLGAYVVRNTAEKPATVVVATGSELSLALEAHERLGGKAGCTDVRIVSM
                     PSRELFLSQPKPVRDAIIPPGVRVVAVEAGAAMGWEALAKREDIFSIDRFGESGPAND
                     VAKHLGFTAENLAKLISG"
BASE COUNT          338 a          777 c          763 g          396 t
ORIGIN      
        1 cgagctcctc ccccgccttg tagagctcgt aggcgcccca catgcccgtc ctggcccaaa
       61 gcgcagagcc gtcctcgaat tcaatgagga ggtggtagct ccgcggggcc gaggcagcag
      121 aggcttcgta gcgcaggtcg cctccgcatt cgccgatgag gaggacatag ccgggctcga
      181 gggggaccga catccatttg cccctgaccc tgggctgtcc tatggtcctg ccttgggcgg
      241 ccgcggcgaa gtccccgggc tcgagattgt gccagacaaa gcggtgctcg acgttcttgg
      301 tcgaggcgcg gcgcagcttc ttgcccagga gctcggagcg tatctgggat gccaggacaa
      361 ggtactcggg caactcgaac atcttccctc ctggacctcc tcagacgata gccttcccct
      421 atcccgacgg caaggcccgc agcctcccgg gggccgtgtt caaaagcccc tcttcgcgct
      481 agcattgcgg catggaaccc agcttcatct cggccaccgt cctcctcatc gtgatcaccg
      541 accccctggg caacatcacc cccgtgctcg ccgccctgcg gggagtcgag cccccgaggc
      601 ggaaggtcat catcctgagg gagtgcgcga tcgccttctt tgtcctcctc ctcttcatgt
      661 tcttcggcca gcagttcctc tcggtcctcc agctcaaaga ggagaccctc aaggtggccg
      721 gaggggtgat cctcttcctc atcgccatca acatggtctt cccgggcagc ggggccaaga
      781 tcgcgggcgc cgagggaggc cgggagccct ttatcgtgcc gatcgccatc cccctcatct
      841 cggggccatc ggccatggtg acggtcatga tcatatccgg atcgaacagc caccgcatgc
      901 tggagtgggt cttcgccctc ctcatcacga tagcggtgag cgcggtcgtg atgctcctct
      961 ccacccgtct gcagcgcatc ctcggcgacc aggtgataac cgccttcgag aggctcatgg
     1021 gcctcgtcct taccgcgatc tcgatcgaca tgctgctgag cggggtggcg gcctacataa
     1081 ggatcctgca gctcgggtcc tgaaggcccg cgggccccgc cgatgcgagg ccggccccgc
     1141 cggcgcgggc ggcacaggaa tgggaaggcc gcccccatgg gggcggcctt cattggctct
     1201 cgggagggcc ctggttagcc cgagatcagc ttagccaggt tctcggcggt gaagccgagg
     1261 tgcttcgcga catcgttcgc tgggccggac tcgccgaagc ggtcgatcga gaagatgtcc
     1321 tcccgcttcg ccagcgcctc ccagcccatc gcggctcccg cctcaaccgc gaccaccctt
     1381 acccccggcg ggatgatggc gtcgcgcacc ggcttgggct gggagaggaa gagctcgcgg
     1441 ctcggcatgg agacgatgcg gacgtcggtg cagccggcct tgccgccgag cctttcgtgg
     1501 gcctcgaggg ccaaggagag ctcggagccc gtcgcgacca ccacggtcgc tggcttctcc
     1561 gccgtgttcc ttaccacata ggcgccgagg cccatggtgt agggccactc ggggtcgtcc
     1621 ttctcgacga cgggcaggtt ctggcggctg agggcgatga cggtcgggcc ctccctcctc
     1681 tccatcgcga ggcgccaagc cacggccgtc tcctcggcgt ccccggggcg gagcacccgc
     1741 aggttcggga tcgcccgaag cgccgcgagg tgctcgatgg gctggtgggt cgggccgtcc
     1801 tcgccgaggt agatcgagtc gtgggtcatc acgtacacga cgggctgcct catgagggcg
     1861 gagagcctca gggccggctt gaggtagtcc gtgaaggtga ggaaggtcgc gacgaagggg
     1921 cgcaggccgc cgtggagcga gatgccgttg gcgatggcgg ccatcgcgtg ctcccgcacg
     1981 ccgaagtaga tcatgcgccc ggccggcgtc gacgccgtat agatcccgcc ctcgggaagc
     2041 tgggtgacgt tggggccggt gaggtcggcc gagcccccca cgaggttggt ccaggccttg
     2101 gcgacggcgc caagagcctt gcccgaggcg ctgcgggtgg cgatcttctc gcccttcgcg
     2161 aaggagggca ggtcgaggtc cttgcgcggc ttcccggaga ggtagctctc cagctccgcc
     2221 gcgagggcgg gctcgtcctt cttccaggcg gcatactcgt tcgaccatgc cgcc
//
LOCUS       DASSQV010000284         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1558762, whole genome shotgun sequence.
ACCESSION   DASSQV010000284 DASSQV010000000
VERSION     DASSQV010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558762"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..763
                     /locus_tag="VFL04_04915"
     CDS             17..763
                     /locus_tag="VFL04_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7839077.1"
                     /translation="MPSSTEKKTAMVTGAAGTLGLVVTKALLERGMRVVMADVDQKRL
                     DELSASLPGETFPFAFNLGDPAQVKAAWTRIEAAVGGVDVLVNNAGILTNNKLETTCS
                     EEWRRVIDVNVNGVFYLSQLVVPGMKARRWGRIINTSSLAAKTGGITAGTAYSTSKGA
                     VISFTFSLAAELAPFGITVNGIAPAYVRTPMVTEQLSEAQRAEVMAKIPVGRFCEPEE
                     FAHVVLFLVDDKAGFITGEIIDQNGGLHFD"
     gene            785..1633
                     /locus_tag="VFL04_04920"
     CDS             785..1633
                     /locus_tag="VFL04_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7839078.1"
                     /translation="MNAARIGELKGIAREIRALTIDEIGYLGVGHIGGAMSVVELLTL
                     LYYEHMVADPQRPRDPERDWLVLSKGHAGPALYATLAHKGFFPRDWLHTLNKGGTSLP
                     SHCDRNLTPGVDMTTGSLGQGFSAAVGIALGLAMQGRPSRVFAVIGDGETDEGQVWEA
                     AMFAAHHRLDKLVAFTDYNKQQLDGMTRDVLDLEDLDAKWRSFGWYTQRVDGHDLRAL
                     DEAVRKAKANEGRPSMIVMDTVKGKGCSFAEGLVSNHNMSYTMDDARAAIAALSAGNV
                     AAGGRA"
     gene            1636..>2177
                     /locus_tag="VFL04_04925"
     CDS             1636..>2177
                     /locus_tag="VFL04_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET7839079.1"
                     /translation="METKEMRAAYAETLVELAASNPEIVVLEADLMRATGTGSFQKAY
                     PDRLVNVGVAEANMVGIASGLSATGKIPFASTFGCFASRRAFDQFFLSANYARLNVKL
                     VGTAPGVTAAFNGGTHMPFEDLALMRAIPGLRIAEPSDPASCAALVREAAALYGCVYL
                     RLPRKAAPYLYGPEESFEFG"
BASE COUNT          386 a          749 c          716 g          326 t
ORIGIN      
        1 atcgacggag gggcaaatgc caagctctac tgagaagaag accgccatgg tcaccggcgc
       61 ggccgggacc ctgggcctgg tcgtcaccaa ggccctcctc gagcggggca tgcgggtggt
      121 catggccgat gtcgaccaga agcgtctgga cgagctctcg gcctccctgc ccggcgagac
      181 cttccccttc gccttcaacc tcggcgaccc cgcccaggtg aaggcagcct ggaccaggat
      241 cgaggccgcc gtgggcgggg tcgacgtcct cgtgaacaac gcgggcatcc tcacgaacaa
      301 caagcttgag accacctgct ccgaggagtg gcggcgggtg atcgacgtca acgtcaacgg
      361 cgtgttctac ctcagccagc tcgtcgtccc cggcatgaag gccaggaggt ggggcaggat
      421 catcaacacc tcgagcctcg ccgccaagac gggcgggatc acggcgggca cggcctactc
      481 gacctcaaag ggcgcggtca tctccttcac cttctccctg gcggccgagc tcgccccctt
      541 cggcatcacg gtcaacggca tcgccccggc ctacgtcagg accccgatgg tgaccgagca
      601 gctctccgag gcccagaggg ccgaggtgat ggccaagatc cccgtgggcc gcttctgcga
      661 gcccgaggag ttcgcccacg tcgtgctctt cctcgttgac gacaaggcgg gcttcatcac
      721 cggcgagatc atcgaccaga acggcggtct tcatttcgac tgaaaagatg ggggagcaat
      781 gctcatgaac gcggcgagga tcggcgagct caagggcatc gccagggaga tcagggcgct
      841 gacgatcgac gagatcggct atctgggggt cgggcacata ggcggggcca tgtcggtggt
      901 cgagctcctc accctccttt actacgagca catggtcgcc gacccgcaga ggccccgcga
      961 tcccgagcgg gactggctcg tgctctccaa gggccacgct ggccctgccc tctacgcgac
     1021 cctggcccac aagggcttct tcccaaggga ctggctccac accctcaaca agggcgggac
     1081 cagcctgccg agccattgcg accgcaacct cacgcccggg gtggacatga cgacgggctc
     1141 ccttggccag ggattctcag ccgccgtcgg gatagccctc ggcctggcca tgcagggtcg
     1201 cccatcgcgg gtcttcgcgg tcatcggcga tggggagacg gacgagggcc aggtctggga
     1261 ggcggcgatg ttcgcggccc accacaggct ggacaagctc gtggccttca ccgactacaa
     1321 caagcagcag ctggacggga tgaccaggga cgtcctcgac ctcgaggacc tggacgcgaa
     1381 gtggcgctcc ttcggctggt acacccagag ggtggacggc cacgacctcc gcgccctcga
     1441 cgaggcggtg aggaaggcga aggccaacga gggcaggccc tcgatgatcg taatggacac
     1501 ggtcaagggt aagggctgca gcttcgccga gggccttgtg tccaaccaca acatgagcta
     1561 cacgatggac gacgccaggg ctgcgatcgc cgccctctcg gcgggcaacg ttgccgcggg
     1621 cgggagggcc tgaagatgga gacgaaagag atgcgcgcgg cctatgccga gaccctggtg
     1681 gagctggcgg cctcaaaccc tgagatcgtg gtcctcgagg ccgacctcat gcgcgccacg
     1741 ggcacgggca gcttccagaa ggcctacccc gaccgccttg tcaacgtggg agtggccgag
     1801 gccaacatgg tgggcatcgc ctcgggcctc tcggccacgg gcaagatccc cttcgcctcg
     1861 accttcggct gtttcgcctc gaggcgggcc ttcgaccagt tcttcctctc ggccaactac
     1921 gcgaggctca acgtcaagct cgtgggcacc gcccccggcg tcaccgccgc cttcaacggc
     1981 gggacccaca tgcccttcga ggacctcgcc ctcatgaggg cgatccccgg cctcaggatc
     2041 gccgagccct cggaccccgc ctcctgcgcg gccctggtgc gcgaggcggc ggccctctac
     2101 ggctgcgtct acctcaggct gccgaggaag gcagccccct acctctacgg gccagaggag
     2161 agcttcgagt tcggcaa
//
LOCUS       DASSQV010000285         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2181704, whole genome shotgun sequence.
ACCESSION   DASSQV010000285 DASSQV010000000
VERSION     DASSQV010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181704"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFL04_04930"
     CDS             <1..667
                     /locus_tag="VFL04_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7839080.1"
                     /translation="TGKRTVGDTVEAAARVSEMSRNLLEANKVIAGIASRTNLLAMNA
                     AIEAAHAGDAGRGFAVVADEIRKLAEQSTSQAGDISKDLERVAESIASVQAASGAAVG
                     AFGKIVDSTRGLGDSVGGIRQSMTEQREGGRQVLEALSKMKDITREITRGAEEMASGT
                     ASILSQVERLRNANFVVVSNTTEISAGTKEINDAVVGTTGLATRNAELIAEVKSAADR
                     FKV"
     gene            681..>2449
                     /locus_tag="VFL04_04935"
     CDS             681..>2449
                     /locus_tag="VFL04_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7839081.1"
                     /translation="MESALMSRLGVRFKLVLLAAVVLLSFLTLVFVSGRAFGQLSAAL
                     GEAHNVRLGLVRLSYEIEVQAYAAQLSLAKSINSGIRGTSLDELGKDLKQLETTIKTG
                     KSLLSQLLAFPLSDEAERLASVALADSYTRYGDMVSQTLSMIRFDPAKAFRNAALLED
                     SENAFSDLEKSLTRLSAEATKGSEEGFLLARRQESASSAIVYAVAAVAMCLVGLVVLL
                     TFRSITLPLGRLVSTVGRMGEGDLTTQTGVTGKDELGRIAGCVDNLVTDLRCLVATVK
                     EKLAELDTTGQSLSSTMSETGASVIQINATIANTKGQLEEQSSAVGEVSAAIEELARS
                     IEALSAMIQRQSETIAQSSAGMERMIAGIESMAEDAERAGEVSERSLAVSAEGKAKID
                     TVGESVGAIVRHSRNLSEAAALITEIAERTNLLAMNAAIEAAHAGESGRGFAVVADEI
                     RKLAEQSTSQARDISRDLALVSEAIESVRAASGAAVDSFAEILRRSGELGGTVGTLRL
                     AAKEQREGGQRVLGSLAEMRNITRQITNGAQEMTAGNSSILGQVERLKTANLVVVRNN
                     EEVSGGTKEINEAVTATIDLSSR"
BASE COUNT          454 a          800 c          850 g          345 t
ORIGIN      
        1 gacggggaag cgaacggtgg gcgacaccgt cgaggcggcg gcccgcgtca gcgaaatgtc
       61 ccgcaacctc ctcgaggcca acaaggtcat cgccggcatc gcctcgcgga ccaacctcct
      121 cgccatgaac gcggccatcg aggccgccca tgcgggagac gccgggcggg gctttgccgt
      181 ggtcgccgac gagataagga agctggccga gcagtcgacc tcccaggccg gcgacatctc
      241 caaggacctc gagagggtgg ccgagtccat cgcctcggtc caggcggcct ccggcgccgc
      301 ggtcggggcc ttcgggaaga tcgtcgacag cacgcgcggg cttggggatt cggtcggggg
      361 catcaggcag tcgatgacgg agcagcgcga gggcggccgg caggtgctcg aggccctctc
      421 gaagatgaag gacatcaccc gcgagatcac gcggggggcc gaggagatgg cttcgggcac
      481 cgcgtcgatc ttgagccagg tggagcgcct caggaacgcg aacttcgtcg tggtcagcaa
      541 cacgacggag ataagcgccg gcacgaagga gatcaacgac gcggtcgtgg gcaccacggg
      601 cctcgcgaca cggaacgccg agctcatcgc cgaggtgaag tcggcggcgg acaggttcaa
      661 ggtctaggaa ggtcaaaccc atggaatcag ccctcatgtc ccgcttgggc gtgcgcttca
      721 agcttgtcct cctcgccgcc gtcgtccttc tgtccttcct caccctggtg ttcgtgtcag
      781 gcagggcctt cggccagctc tcggcagccc tgggcgaggc gcacaacgtg cgcctgggac
      841 tggtccgcct cagctacgag atagaggtcc aggcctacgc ggcccagctc agcctggcga
      901 aatccataaa cagcgggatc cgcggcacaa gcctggacga gctagggaag gacctgaagc
      961 agctcgagac gaccatcaag accgggaagt cccttttgtc ccagctcctc gccttccccc
     1021 tgtcggacga ggccgaaagg ctcgcctcgg tggcccttgc ggattcctac accaggtacg
     1081 gggacatggt cagccagacc ctcagcatga tcaggttcga tccggccaag gccttcagga
     1141 acgccgccct cctcgaggat tccgagaatg ccttctcgga cctcgagaaa tccctcacca
     1201 ggctctcagc cgaggccacg aagggcagcg aggagggctt cctcctcgcg cgccgccagg
     1261 agtccgcctc ctcggcgatc gtctacgccg tggccgccgt cgcgatgtgc ctcgtgggcc
     1321 tcgtcgtcct tctgaccttc aggtcgatca ccctgcccct cggcaggctc gtgtccaccg
     1381 tgggcaggat gggcgagggc gacctcacca cccagacggg cgtgacggga aaggacgagc
     1441 tcgggcgcat cgccggctgc gtcgacaacc tggtgaccga tctgcgctgt cttgtggcga
     1501 ccgtcaagga gaagctggcc gagctcgaca cgacgggcca gagcctgtcc tcgaccatgt
     1561 cggagacggg agcctcggtg atccagatca atgcgaccat cgcgaacacg aaaggtcagc
     1621 tcgaggagca gagctcggcg gtgggggagg tctcggccgc gatcgaggag ctcgcccgca
     1681 gcatcgaggc cctctccgcg atgatccagc gccagtccga gacgatagcc cagtcctcgg
     1741 cgggcatgga gcggatgata gccggcatcg agtccatggc cgaggacgcg gagcgggcgg
     1801 gcgaggtgag cgagcgcagc ctcgccgtca gcgccgaagg caaggcgaag atcgacacgg
     1861 tgggcgaatc ggtgggggcc atcgtgcgcc actccaggaa cctcagcgag gccgccgccc
     1921 tcatcaccga gatcgccgag aggaccaacc tcctggccat gaacgccgcc atcgaggcgg
     1981 cccatgccgg ggaatcgggc aggggcttcg ccgtcgtggc cgacgagatc aggaagctgg
     2041 ccgagcagtc cacctcgcag gcgagggaca tctcgaggga cctcgccctg gtgtcggagg
     2101 ccatcgagtc agtgcgcgcc gcctccgggg cggccgtgga ctccttcgcg gaaatcctca
     2161 ggcgctcggg cgagcttggc ggcaccgtcg ggaccctgcg tctggcggcc aaggagcagc
     2221 gtgagggcgg gcagcgtgtg ctcgggtccc tggccgagat gcggaacatc acgaggcaga
     2281 tcacaaacgg ggcccaggag atgaccgcgg gcaactcctc gatcctgggc caggtcgagc
     2341 gcctcaagac cgcaaacctc gtcgttgtca ggaacaatga ggaggtcagc ggggggacca
     2401 aggagatcaa cgaggcggtc acggccacca tcgacctgag ttcgaggaa
//
LOCUS       DASSQV010000286         4625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2430297, whole genome shotgun sequence.
ACCESSION   DASSQV010000286 DASSQV010000000
VERSION     DASSQV010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2430297"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VFL04_04940"
     CDS             complement(<1..1016)
                     /locus_tag="VFL04_04940"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET7839082.1"
                     /translation="MKKRIVLDQIRPGSRFSDDVFIDEKNLLVPANVAVKQRDLDTLK
                     RWGVNFVITEGGLIEEAAAKQGLAQGEAAKLPQGFVGSRELYGLYTGLVEGLGQVFAR
                     IRGNEAVETKAVDQLSQGVLGLLRDRKDATLSAILSAETSEYNPARAAVDCAILSVVI
                     GTALRLPPYRLGYLATGALLHDAGMLRIPESILRKNGSLTEEEAQKIRAHPLLSYRIV
                     TKDLLYPDDVGLIGLQHHERWDGDGYPRKTGGQEIDVLARIVSVADAFEAMVSSKPYR
                     NSMIGYAAMKALLSDNSRRFDPDIIKAFIKSMGIYPLGSTVLLNNAAIARVVETHPDA
                     PLRP"
     gene            complement(1013..1288)
                     /locus_tag="VFL04_04945"
     CDS             complement(1013..1288)
                     /locus_tag="VFL04_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HET7839083.1"
                     /translation="MPREGRASRRSAKAIALRYDTGLPAPFLAAKASGRAVDRLLAIA
                     EEAGLPMIRDGALTDALYPLDLGDYVPEAYFEIVAKVFAFVKSIEEA"
     gene            complement(1281..2369)
                     /locus_tag="VFL04_04950"
     CDS             complement(1281..2369)
                     /locus_tag="VFL04_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839084.1"
                     /translation="MRTAGPVEATFVVAGAGQGQKLRAGALVSVTVLESRSPGSYLVQ
                     VGGRRLSASGPAGLQPGSLFRAKVESTPGGLILRAMAPPRQAGAGMAEILARSGLPDD
                     RASRLALTALLGEGLRPQAPALSRVRRAFARAGGEGEESASGSELGRLAARMEAAGLG
                     SEDGILEALAAETGLPSGSGGGGGAQGGDGSGTGPGGGQKTAGRRLELAGDELPAVLG
                     ALLRQTVLGSGGEGSLLNLFNHARGAEGSWIYAPFRFELDSVAFSGCFRVKLPLQMGG
                     PGRIEADFEATRRGSEPRTWSLGLAFGGSGRSALGIRANAGRDKALAAADLAGFEADL
                     AASGCSLKLVSDEAGEDPLPASGLELDA"
     gene            complement(2393..2764)
                     /gene="rplS"
                     /locus_tag="VFL04_04955"
     CDS             complement(2393..2764)
                     /gene="rplS"
                     /locus_tag="VFL04_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET7839085.1"
                     /translation="MNLIQKIQAPQLRSDLAAFSVGDTVKVHFKIVEGKNERVQIYEG
                     LVIAMKNAGLGKTFMVRKNSYGVGVERVFPMNSPRIEKVEVVRLGKVRRAKLYYIRTK
                     IGKKSKVKEYIPVKKDAAAKA"
     gene            complement(2706..3503)
                     /gene="trmD"
                     /locus_tag="VFL04_04960"
     CDS             complement(2706..3503)
                     /gene="trmD"
                     /locus_tag="VFL04_04960"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET7839086.1"
                     /translation="MNIKVLSLFPGILRGFFESSIMAKAVARGLMSYELTDIRDYATD
                     KHHKADDQVFGGGAGMLMLPGPLSRALEAAGSPRVRTVYVTPSGRLLNQAYARELAAE
                     KDLVIVCGRYEGIDQRVIDSYVTDEISIGDYVLSSGEVAALVLVDAVYRLVPGVISGE
                     SLEEESFGSGERHALLEYPQYTRPAEYASMRVPEVLISGHHAKIAKWRLRASLEKTLA
                     FRPELLAVGGLPDEVRKLLIEIAAEGRNHEPDTEDPGAPAEVRPRGV"
     gene            complement(3500..4078)
                     /gene="rimM"
                     /locus_tag="VFL04_04965"
     CDS             complement(3500..4078)
                     /gene="rimM"
                     /locus_tag="VFL04_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET7839087.1"
                     /translation="MEKKESPADRIAIGRLGAPKGVRGDLKVHSYSGESEHFLALKEV
                     ELASTGLVERRLRLKVLRVEGEGASLTMAFEGYPSPEAARVLTGMDIMVGPEGIAPLR
                     KNEWYISDLVGLAVVAEGREVGRVLSVLEGGPEPWLEVQRNAEDRMPSASEGSAKRNA
                     AGVSLVPFRKEFVGEVDVAAGRLELIAPWILE"
     gene            complement(4122..4355)
                     /locus_tag="VFL04_04970"
     CDS             complement(4122..4355)
                     /locus_tag="VFL04_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET7839088.1"
                     /translation="MERDLVEYIAKSLVDDPASVNVAVVEGDKSTILELRVAADDIGK
                     VIGKHGRIAKAIRTILSASSGASEKRVMLEILD"
     gene            complement(4446..>4625)
                     /gene="rpsP"
                     /locus_tag="VFL04_04975"
     CDS             complement(4446..>4625)
                     /gene="rpsP"
                     /locus_tag="VFL04_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET7839089.1"
                     /translation="SRKPRDGRSLEEIGLYHPIEVPEKQLVIDEARAKEWLAKGAQPT
                     DTVKSLLNSKGIHVK"
BASE COUNT          677 a         1561 c         1593 g          794 t
ORIGIN      
        1 ttgggccgga gcggcgcgtc ggggtgcgtt tccacgacgc gcgcgatcgc cgcattgttg
       61 agcagcaccg tcgagccgag cggatagatg cccatgctct tgatgaaggc cttgatgatg
      121 tcgggatcga agcggcgcga gttgtcggac agcagggcct tcatggccgc gtagccgatc
      181 atggagttgc ggtagggctt ggagctgacc atggcctcga aggcgtcggc aaccgagact
      241 atcctggcca ggacgtcgat ctcctggccc ccagtcttcc ggggatagcc gtcgccgtcc
      301 cagcgctcat ggtgctggag gccgatgagt cccacgtcgt cgggatagag caggtccttg
      361 gtgacgatcc ggtaggacag cagggggtgg gccctgatct tctgggcctc ctcctcggtc
      421 aggctgccgt tcttcctgag gatcgactcg gggatcctca gcatccccgc gtcgtggagg
      481 agggcccccg tcgcgaggta gccgagcctg tagggcggga gcctcagggc cgtcccgatg
      541 acgacggaga ggatggcgca atcgacggcg gcgcgggccg ggttgtattc gctggtctcg
      601 gcgctcagga tcgcggagag ggtggcgtcc ttccggtcgc ggaggaggcc gagcacgccc
      661 tgggagagct ggtccacggc cttggtctcg acggcctcgt tgccccggat cctggcgaag
      721 acctggccga gcccctccac gaggcccgtg tacaggccgt aaagctcgcg gctcccgacg
      781 aaaccctggg ggagcttggc ggcctcgccc tgggccaggc cctgcttcgc ggcggcctcc
      841 tcgatgagac ctccctcggt gatcacgaaa ttcacgcccc agcgcttgag ggtatcgagg
      901 tctcgctgct tgaccgccac gttggcgggg accaggaggt tcttctcgtc gatgaagaca
      961 tcgtccgaga atcgcgaccc gggccgtatc tggtccagga cgatacgttt cttcatgctt
     1021 cctctatcga cttcacgaag gcgaagacct tcgctacgat ctcgaaatac gcctcgggga
     1081 catagtctcc gagatccagg ggatagaggg cgtcggtgag ggccccgtcc ctgatcatcg
     1141 gcagccctgc ctcctcggcg atggccagca ggcggtccac ggcgcgcccc gaggccttcg
     1201 cggccaggaa gggagcggga agccccgtgt cgtaccggag cgcgatcgcc ttcgcgctcc
     1261 ggcgggaagc cctgccttcc ctaggcatcg agctcaagcc ccgatgccgg gaggggatcc
     1321 tcccccgcct cgtcggaaac cagcttcagc gagcagcccg aagcggccag gtcggcctcg
     1381 aaacccgcca ggtcggcggc cgcgagcgcc ttgtcgcgcc cggcatttgc gcggatgccg
     1441 agggccgatc gtccagagcc cccgaaggcc agcccgaggg accaggtccg gggctccgag
     1501 ccccgtctgg tcgcctcgaa atccgcctcg atcctgccgg ggccccccat ttgtaatggc
     1561 aattttactc gaaagcagcc cgagaatgca accgaatcga gctcgaagcg gaagggcgcg
     1621 tagatccagg agccctcagc ccctctggca tggttgaaca ggttgaggag gctgccctcg
     1681 ccccccgagc cgagcacagt ctgtcggaga agggcgccga ggacggccgg cagctcgtcc
     1741 ccggccagtt cgagcctcct cccggcggtc ttctggccgc ccccggggcc ggttcccgat
     1801 ccgtccccgc cctgcgcccc tcccccgccg cccgagccgg aagggaggcc cgtctcggct
     1861 gccagggcct cgaggatccc gtcctcggag ccgaggccgg ccgcctccat ccgggcggcg
     1921 agtctgccga gctcggagcc gctcgcgctt tcctccccct cgccgccagc acgggcgaag
     1981 gccctccgca cccgggaaag ggccggggcc tggggccgca gcccctcgcc gaggagggcg
     2041 gtcagggcga ggcgggatgc tctgtcgtca gggaggcccg agcgggcgag gatctcggcc
     2101 atcccggccc cggcctgacg gggcggggcc atggcgcgca ggatgaggcc gcccggggtc
     2161 gactcgacct tggcgcggaa cagggagccc ggctggaggc cggccgggcc cgaggccgag
     2221 agccgcctgc cgcccacctg gaccaggtat gagccgggcg agcggctttc cagtacggtc
     2281 acggatacga gagcgccggc ccggagcttc tgcccctggc cggcgcccgc gacgacaaag
     2341 gtcgcctcga ccggacccgc ggtccgcatc gccctctcct gccggtctgg ccctaggcct
     2401 tggcggcggc gtccttcttg acggggatgt attccttgac cttggacttc ttgccgatct
     2461 tggtgcggat gtagtagagc ttcgcgcggc ggaccttgcc gagccgcacg acctcgacct
     2521 tctcgatccg gggcgagttc atggggaaga cgcgctcgac gccgacgccg taggagttct
     2581 tcctcaccat gaaggtcttg ccgaggccgg cgttcttcat cgcgatgacc agaccctcgt
     2641 agatctggac gcgctcgttc ttgccctcga cgatcttgaa gtggaccttg accgtgtcgc
     2701 caacgctaaa cgccgcgagg tctgacctca gctggggcgc ctggatcttc tgtatcaggt
     2761 tcatggtttc tcccttcggc tgctatttca ataagaagtt tccggacctc atcgggaagc
     2821 ccgcccaccg cgagaagctc gggtcggaag gccagggtct tctcaagact cgccctgagc
     2881 cgccacttcg ctattttcgc gtggtgaccc gagatcagga cctcggggac tcgcatacta
     2941 gcatactcgg cggggcgtgt atactgggga tactccagga gggcgtgccg ctcgcccgag
     3001 ccaaagctct cctcctcgag ggattccccc gagatcaccc cgggcacgag gcggtagacc
     3061 gcgtcgacga ggacgagggc agccacctcc ccggaggaca ggacatagtc cccgatcgat
     3121 atctcgtcgg tgacgtagct gtcgatcacc cgttggtcga tgccctcgta gcgtccgcag
     3181 acgatgacga ggtccttctc ggccgcgagc tcgcgggcgt aggcctggtt gaggagccgg
     3241 cccgaggggg tcacgtagac cgtcctcacc cgcggagagc ccgccgcctc gagggcgcgg
     3301 ctcagggggc ccggcagcat gagcatgccc gcccctccgc cgaagacctg gtcgtcggcc
     3361 ttgtggtgct tgtcggtcgc gtagtccctg atgtcggtga gctcgtagga catgaggccc
     3421 ctggccacgg ccttggccat gatcgagctc tcgaagaagc cccggaggat gccggggaag
     3481 aggctcagga ccttgatgtt cattcgagga tccagggcgc gatgagctcg agccgcccgg
     3541 ccgccacgtc gacctcgccc acgaactcct tcctgaaggg caccagcgaa acgcctgcgg
     3601 cgtttcgctt cgcgcttcct tcggaagcgc ttggcatccg gtcttcggcg tttcgctgga
     3661 cctcgagcca gggctcgggc cctccctcga ggacggacag cacccgcccg acttccctgc
     3721 cctcggcgac gacggcgagg cccacgaggt cggagatgta ccactcgttc ttgcggaggg
     3781 gggctatgcc ctcggggccc accatgatgt ccatgccggt gaggacccgc gccgcctcgg
     3841 ggctgggata gccctcgaaa gccatggtga gggaggcccc ctccccttcg accctgagga
     3901 ccttcagcct gagcctgcgt tcgacgaggc cggtcgaggc gagctcgacc tccttgagtg
     3961 cgaggaagtg ctccgactcg cccgagtagg agtggacctt gaggtcgccc ctgacgccct
     4021 tgggcgcgcc gaggcggccg atggctatgc ggtcggcggg gctctctttc ttctccatgg
     4081 cggtgtgggt tcggggggcc gccccggatg gggcgccagc cctagtcgag gatctcgagc
     4141 atcacccgct tctcggaggc gcccgaggag gcggagagga tggtcctgat ggccttggcg
     4201 atgcggccgt gtttgccgat caccttgccg atgtcgtccg cggcgacgcg gagctcgaga
     4261 atggtggact tgtcgccctc gacgaccgcg acgttgacgg acgcgggatc gtcgacgagg
     4321 gacttcgcga tgtattccac gagatcgcgt tccacggtgg tctccttaag gattccccgg
     4381 tacggcgccg gccgcccggg gcgtgggccg ccgcccggct ggtcgccggc ggcggggcct
     4441 ctggcctatt ttacgtggat tcccttgctg ttgaggaggc tcttcacggt gtccgtgggc
     4501 tgggcgccct tggcgagcca ctccttagcc ctcgcctcgt cgatgacgag ctgcttctcg
     4561 ggcacctcga tcgggtggta aagcccgatc tcctcgaggc tgcggccgtc gcggggcttg
     4621 cggga
//
LOCUS       DASSQV010000287         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_564984, whole genome shotgun sequence.
ACCESSION   DASSQV010000287 DASSQV010000000
VERSION     DASSQV010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564984"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1302)
                     /locus_tag="VFL04_04980"
     CDS             complement(49..1302)
                     /locus_tag="VFL04_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HET7839090.1"
                     /translation="MAKSLASAYRSIVSDPFPPRVELAFYDEAGTRTALVYEKVEWDV
                     EGKRRGLRYGENPDQAAALYRPVGGNLALAGAVLVEPGEGLVSTAELIQFGKHPGKTN
                     LGDVDAALGILRYLPGRPACAVMKHNNPSGLAQGPSLSAAYRRAWLADRVAAFGGAVV
                     LNRSLDRDTATAISSSYCEVVAAPDFEEGSIGILASRKNLRIMRIPGISRLERWALAP
                     QLDFSSMTDGAFIVQTAFLSRIRSRGDFLPAAAEREGRTYAVAREPTEAEWEDMLFGW
                     AVETGVTSNSVIYVKDGATVGIGTGEQDRVGVAEIARDKAYRKLEDRLLWERTGLGWN
                     ESSDEGAKAAVREEAASLRGGLAGSVMVSDAFFPFRDGIEVGLREGVSAVVQPGGSMR
                     DFEAIEACNEYGASMKFTGQRCFRH"
     gene            complement(1307..1978)
                     /locus_tag="VFL04_04985"
     CDS             complement(1307..1978)
                     /locus_tag="VFL04_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839091.1"
                     /translation="MPFNKVARALDLVPLRKALVSVYDKKGLPEFARGLMEACPEMRF
                     YATGGSYTALRDSVDPQSVVSIGDYTGQSEMAGGLVKTLDWRIYLGLLAETGDERHEA
                     DLARHKAVAFDLVVVNLYPFAASAADSTSTPEELRQRIDIGGSAMLRAAAKNYLRVAA
                     LSGPGQYRGFLSELDAQGGWTGLEYRRRLAVEAFALTARFDEEVSRRLAGIEAAAIEA
                     AYGLG"
BASE COUNT          293 a          696 c          724 g          328 t
ORIGIN      
        1 cgcgcgcgaa agctcctggg atctgctttc gcgaaggtca gcggtgcgtc aatgcctgaa
       61 gcaccgctga ccggtgaatt tcatgctagc cccgtattcg ttgcaggcct cgatcgcctc
      121 gaagtcgcgc atggagcccc caggctggac gacggcgctc acgccttccc tcaggcccac
      181 ctcgatgccg tcccggaagg ggaagaaggc gtcggagacc atgaccgatc ccgcgagacc
      241 gccgcggagg gaggccgcct cttcgcgcac ggcggccttg gccccctcgt cgctggactc
      301 gttccagccc aaacccgttc gctcccagag gagcctgtcc tcgagcttgc ggtaggcctt
      361 gtccctggct atctcggcga ccccgaccct gtcctgctcg cccgtgccga tgccgaccgt
      421 cgccccgtcc ttcacataga tgacggagtt gctcgtgacc cctgtctcca ccgcccatcc
      481 gaatagcatg tcctcccact cggcctcggt cggttcgcgg gcgacggcgt aggtccttcc
      541 ctccctctcg gcggcggcag ggaggaaatc ccccctcgac ctgatgcgcg agaggaaggc
      601 ggtctggacg atgaaggcgc cgtcggtcat ggacgagaag tcgagctggg gggcgagggc
      661 ccagcgctcg agccgggata tccccggtat cctcatgatg cggaggttct tcctcgaggc
      721 gagtatcccg atcgacccct cctcgaagtc gggggccgcg acgacctcgc agtacgagga
      781 ggagatcgcc gtggcggtgt cgcggtcgag gctgcggttg aggaccacgg ccccgccgaa
      841 ggccgccacc ctgtcggcga gccaggccct ccggtaggcc gcgctgaggc tggggccctg
      901 ggcgaggccc gaggggttgt tgtgcttcat gacggcacag gcggggcggc ccggcagata
      961 gcggaggatg ccgagggcag cgtcgacgtc gccgaggttg gtcttgcctg ggtgtttgcc
     1021 gaactggatg agctcggccg tcgagaccag gccctccccc ggctcgacga ggactgcgcc
     1081 ggcgagggcg aggttgcccc cgacggggcg gtagagcgcc gccgcctggt cggggttctc
     1141 gccgtagcgg aggccgcggc gctttccctc gacatcccac tccaccttct catagacgag
     1201 ggccgtcctc gtcccggcct cgtcgtagaa ggccagttcc acccggggcg ggaaggggtc
     1261 cgagacgatg cttctgtagg ccgaggccag gctcttcgcc atgggcctag cccagaccgt
     1321 aggccgcttc gatcgcggca gcctcgatcc cggcgaggcg gcgcgagacc tcctcgtcga
     1381 agcgggcggt gagggcgaag gcctcgaccg ccagcctccg gcggtactcg agcccggtcc
     1441 agccgccctg ggcgtcgagc tcggagagga agccgcggta ctggccgggg cccgacaggg
     1501 ccgccacgcg gaggtagttc ttcgccgcgg cgcgcagcat cgcgctgcct ccgatgtcga
     1561 tccgctgacg gagctcctcg ggcgtcgacg tggagtcggc ggccgaggcc gcgaagggat
     1621 agaggttgac gacgacgagg tcgaaggcga cggccttgtg cctggccagg tcggcctcgt
     1681 ggcgctcgtc ccccgtctcg gcgaggaggc cgaggtagat ccgccagtcg agggtcttca
     1741 cgaggccccc cgccatctcg ctctggcccg tgtagtcgcc gatcgacacg actgactgcg
     1801 gatctacgga gtcgcggagg gccgtgtagc taccccccgt cgcgtagaag cgcatctcgg
     1861 ggcaggcttc catgaggccg cgcgcgaact cgggcaggcc cttcttgtcg tagaccgaca
     1921 cgagggcctt cctgagcggg acgaggtcga gcgcccgggc taccttgttg aagggcatga
     1981 atggctcctt gggggacgtg gccttactgc gggccgctct ggcccgaggc atcggtcgag
     2041 c
//
LOCUS       DASSQV010000288         7697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1559838, whole genome shotgun sequence.
ACCESSION   DASSQV010000288 DASSQV010000000
VERSION     DASSQV010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559838"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..927)
                     /locus_tag="VFL04_04990"
     CDS             complement(157..927)
                     /locus_tag="VFL04_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET7839092.1"
                     /translation="MLIDWHTHLSSYTDIDGALRTIRAERILSVACSVDAESYIETRA
                     LARGQGFVIPTFGVHPAKSGSVRDLASLDPYLDESRIIGEIGLDDFWVKDVSQAVQEP
                     VFEYMLDHCDRRGKYCVIHTKGAEARIADMLGHFPGCRPIIHWYSGPKDLLIALLDLG
                     CLFTFGLELHYSDHIAELLDLVPPDRLLSETDNPGSEPWLGGNTDSPRLIERVVREIA
                     SRKGMRPLDMENLIWRNSMRILRESGVEADLEPSSPRP"
     gene            complement(972..1529)
                     /locus_tag="VFL04_04995"
     CDS             complement(972..1529)
                     /locus_tag="VFL04_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7839093.1"
                     /translation="MIIKAMRRESASDWFEFFDNRAFADHEDWKGCYCTGFFMPRLEA
                     YPGRAIKRKDYAAWLIEHELMKGYLGYENGRVVAWCNVNRKSAFPKLKEINEGGEEVL
                     SIACFLVEKPYRRRGIAQKLLSRIIKDAKQEGIAIIEAYPRPRAQTEYGKYRGPFSMY
                     EKNGFKVERVMGMDVMRRHLAAGRG"
     gene            1621..3888
                     /gene="ptsP"
                     /locus_tag="VFL04_05000"
     CDS             1621..3888
                     /gene="ptsP"
                     /locus_tag="VFL04_05000"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET7839094.1"
                     /translation="MITGAIEEVAARIALFDDEGAQKCLLRDLVDLVVKRSGADMGAV
                     FVHDEAQAQLVLRAGFERGELSSDERLKGGRTPMRFGLEGNEVGRAFTERRIIWLSYA
                     EAEPGHPYRSKVLVPIIRGPIQLGVLILARADARGFDREDEAALMAAASRFGELLSEA
                     SALLAGREADAGGASARSIQGIKASDGRAYGIALPFWADMDALADQALARAEPATELA
                     GFEAALSASVRQLGDLRSGAASADFEMVAMIFSAHELMLKDAGFTDRMRRRIEGGQSA
                     VQSVQAVVAEYAERFAAVSEIRIAEKAQDVRDLGYRLINNLLGGSPEGFSYRGKIAIA
                     RHIYPSDLYRLAVEGVEGLVLRGSGVTAHIAILARSLALPVMITDDKGILGIPEGTPL
                     LLDATDGWLHVNPGKAKLDHYRSTRGMVTELRAYHLKGRTADGTGVQVLANVNILKDA
                     LEATAQGAEGIGLYRSEFPFILKNDFLSEEQQYKIYRSIVASQPGKPVVLRTADIGGD
                     KLLQGRGSAETNPFLGVRGIRFSLANREMFRDQLRAMLRAGEGADLGIMLPMVSGVEE
                     VLEAKQEIALAAAALAERRVAHNSAPRIGAMIELPSAAMAVGDIAGETDFLSIGSNDL
                     TMYLLAVDRTNEKLHHLYRTHHPAVLRVFSAIARYSGSKRAELSVCGDVASDPVLAPF
                     FVGIGIRKLSVSPAKVEAVKRRLASFTLGEMQAISRELLAIRRLADMERYLADFEGRH
                     PGIPAEASGLSVAIG"
     gene            complement(3979..4476)
                     /locus_tag="VFL04_05005"
     CDS             complement(3979..4476)
                     /locus_tag="VFL04_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7839095.1"
                     /translation="MYNRPTSILLIEDDPILAMGLAVELQDHGFDVNNAYSGEGAIEA
                     LRMGMAPSDLILMDLDLGRGLDGAQTAKQILADFDIPIVFLSSHSEDEFTALTERIPS
                     YGYLLKDNGTPIIVAAVRTALKEYKSKRRARENPVGEPSANIRPELRVPAPGRRPPSR
                     GKTSF"
     gene            complement(4568..5635)
                     /locus_tag="VFL04_05010"
     CDS             complement(4568..5635)
                     /locus_tag="VFL04_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET7839096.1"
                     /translation="MGKEPHSILLVEDDPIVAMGLSVELQDEGFRVNSAYSGEMAIAA
                     LRQGIAASDLILMDLDLGIGIDGVQAASEILRDHDIPIVFLSSHSEREFAARTEKVPS
                     YGYILKTNGAPVIAAAVKMALAVHERCRGAPRLSPFRNMKRRAEQSLRAALEEKDVLL
                     KELQHRTKNNLGLISSLLTLSLNVSGDESSHRALLEARSRVHAMAAVYETLTYSDSVE
                     SVDLEAYFRRFIASLCEGYVIDRDMIAIRTRLEAGELDPKRAVTIGLILNELLTNSLK
                     HAYKPGSRGEILVEVLRRGEELELRVSDQGRGLPPGLDPSLSEGMGFTLVRLLTHQLE
                     GRVQFESEAGNGTRVSVLIGN"
     gene            complement(5743..6696)
                     /locus_tag="VFL04_05015"
     CDS             complement(5743..6696)
                     /locus_tag="VFL04_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HET7839097.1"
                     /translation="MNDLRIAVAQFEHRNGDKGYNLSVIERLSAEAAKAGADAIAFHE
                     CSITAYSFARRLSREQMLELAEPIPGGRSVAELQRIASLHRIAILAGLFEKDHEGRLY
                     KAYVCVDRSGLRAKFRKLHPFINEHLSPGNEYVVFELKGWKCGILICYDNNVIENVRA
                     TALLGAELIFMPHVTMCTPSPRPGAGFVDRALWDNRRKDPTSLRAEFDGLKGRSWLMK
                     WLPARAYDNGVFVAFSNPIGMDDDQLKNGSSMILDPFGDILAECRSFDDQFVVADLVH
                     QKLADSGGSRYIRARRPELYRDIIGRDHLPEQKVAWLDSEE"
     gene            6793..7122
                     /locus_tag="VFL04_05020"
     CDS             6793..7122
                     /locus_tag="VFL04_05020"
                     /inference="COORDINATES: protein motif:HMM:NF038084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HET7839098.1"
                     /translation="MDIKDIPFQVFDLANAAPEEHAGETGRAVWRTVKRGELRIRIVE
                     YSAGYLADHWCEKGHAVYVLEGEFESELKDGRRHTLGPGMCYLVADHAEPHRSYTRNG
                     VKLLIVD"
     gene            complement(7109..7333)
                     /locus_tag="VFL04_05025"
     CDS             complement(7109..7333)
                     /locus_tag="VFL04_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839099.1"
                     /translation="MNGTIDMRGSATISKLMEVLTEARWSGALSPGEHQWILKNVVRV
                     AIEQLHAHPTGHFMRVQNPARGLRSLASPR"
     gene            complement(7507..>7697)
                     /locus_tag="VFL04_05030"
     CDS             complement(7507..>7697)
                     /locus_tag="VFL04_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7839100.1"
                     /translation="DGIEAAQRIRESADIPIIFMTGYQDKGTRERARALEPLAFLEKP
                     VRLGDLQSILRSVQTGSP"
BASE COUNT         1264 a         2547 c         2519 g         1367 t
ORIGIN      
        1 cgcgaaggtc gccgagtacc ttcggtcctt caggctcaat cctgcccggg ccgagggagt
       61 cgatcttgcc tcgagctata aggccaagtt caggaaggtc ttcgggatcg agttcgccga
      121 ggcgctgaag ctgctcgacc ccacccccgc gaaaaactag ggtctgggcg agctcggttc
      181 gagatcggcc tcgaccccgc tctccctgag gatcctcatg ctgttcctcc agatgaggtt
      241 ctccatgtcc agagggcgca ttcccttgcg ggacgcgatc tcccggacca cccgctctat
      301 cagcctcggg ctgtccgtgt tcccacccag ccacggctcg ctgcccgggt tgtccgtctc
      361 tgacagaagt cgatcgggcg ggacgaggtc caggagttcg gcgatgtggt cagagtagtg
      421 cagctcgagt ccgaacgtga acagacagcc cagatccaag agggcgatga ggaggtcctt
      481 tgggccgctg taccaatgga tgattggcct gcagcccggg aaatgcccga gcatgtcagc
      541 gatcctcgcc tccgctccct ttgtgtggat gacacagtat ttcccccgtc tgtcgcagtg
      601 gtccagcatg tactcgaaga cgggctcctg cacggcctgc gatacatcct tcacccaaaa
      661 atcatcgagc ccgatttcac cgatgatcct ggattcgtcc aggtaggggt ccaggctggc
      721 caggtccctc acgctgccgc tcttcgctgg gtgcacgccg aaggtcggga tcacaaagcc
      781 ctggccgcgg gcgagggccc gcgtctcgat ataggattca gcgtcgacgc tgcatgccac
      841 gctgaggatc ctctcggccc tgatggtacg gagagcgccg tcgatgtccg tgtacgaact
      901 caggtgggta tgccaatcga tgagcaaagg ggcgccctca aaccaccggc ttcgccgcgc
      961 gcgagccgag cctagccccg ccccgcggcg agatgccttc gcatcacatc catacccatc
     1021 accctttcga ccttaaagcc gttcttctcg tacatcgaaa agggcccgcg gtatttgccg
     1081 tactcggtct gggcccgcgg ccgggggtat gcctcgatga tggcgatgcc ctcctgtttc
     1141 gcgtccttga tgatgcggct aagcagcttc tgggcgatcc cccttcgccg gtagggtttc
     1201 tcaacgagga aacaggcgat ggacaggacc tcctcgcccc cctcattgat ctctttgagc
     1261 ttggggaagg ccgacttccg gttgacgttg caccaggcga ccaccctgcc gttctcgtag
     1321 ccgaggtagc ccttcatcag ctcgtgctcg atgagccagg cggcgtagtc cttgcgcttg
     1381 atcgccctgc cggggtaggc ctcgagcctg ggcatgaaga agccggtgca atagcagccc
     1441 ttccagtcct cgtgatccgc gaaagcccga ttgtcgaaga actcgaacca gtcggaggcg
     1501 ctttcgcgcc gcatcgcctt gatgatcatg ccctgcagta tatccctgcc cagggggggc
     1561 gaccataccg cctttccccc cgcctgggga ttgcgtacct tctaggccat ggatccaggc
     1621 atgatcaccg gcgccatcga ggaggtcgcc gcaaggatag cccttttcga cgacgaaggg
     1681 gcgcagaagt gcctcctcag ggacctcgtc gacctcgtcg tgaagcgctc gggcgccgac
     1741 atgggggcgg tcttcgtgca cgacgaggcc caggcccagc tcgtgctccg cgcgggcttc
     1801 gagcgggggg agctctcgtc cgacgagcgg ctcaagggcg gcaggacccc gatgcgcttt
     1861 gggctcgagg ggaacgaggt cggaagggcc ttcaccgaga ggcggatcat ctggctcagc
     1921 tatgccgagg ccgaacccgg ccatccctac cgctccaagg tccttgtgcc catcatcagg
     1981 ggacctatcc agctcggggt cctgatcctc gcgagggccg acgcgagggg cttcgaccgc
     2041 gaggacgagg ctgccctcat ggcggccgcc tcgcgtttcg gcgagctcct gagcgaggcc
     2101 tcggccctcc tcgcgggccg ggaggccgac gcgggcggag cctcggcgcg ctcgatccag
     2161 ggcatcaagg cttctgatgg ccgggcctac gggatcgccc tgcccttctg ggccgacatg
     2221 gacgccctcg ccgaccaggc cctcgccagg gccgagcccg cgaccgagct cgcgggcttc
     2281 gaggccgcgc tctcggcctc ggtgcggcag ttgggcgatc tcaggagcgg ggcagcctcg
     2341 gccgacttcg agatggtcgc catgatcttc tcggcccacg agctcatgct caaggatgcg
     2401 ggtttcacgg acaggatgcg ccgcaggatc gagggcggcc agtcggccgt gcagtcggtc
     2461 caggccgtcg tcgccgaata cgccgagcgc ttcgccgcgg tttcggagat caggatcgcc
     2521 gagaaggccc aggacgtccg cgacctgggc taccgcctga tcaacaacct cctgggcggc
     2581 tcgcccgagg gcttctcgta caggggcaag atcgcgatag cccgccacat ctacccatcc
     2641 gacctctacc gcctcgccgt cgagggggtg gagggcctcg tcctccgcgg ctcaggcgtc
     2701 accgcccaca tcgccatcct tgccaggtcc ctcgccctgc cggtcatgat caccgacgac
     2761 aagggcatac tcggcatacc ggaggggacg ccgcttctcc tcgacgccac ggatggctgg
     2821 ctccacgtca atcccggcaa ggccaagctc gaccactacc gctccacccg cggcatggtc
     2881 accgagctgc gggcctatca cctcaagggc aggaccgcag acgggacagg ggtccaggtc
     2941 ctcgccaacg tcaacatcct caaggatgcc ctcgaggcca ctgcccaggg cgccgagggc
     3001 attggcctgt accgcagcga gttccccttc atactcaaga acgacttcct ctccgaggag
     3061 cagcagtaca agatctaccg ctccatcgtg gcgagccagc cgggcaagcc cgtggtcctc
     3121 cgcaccgccg acatcggagg ggacaagctc ctccagggcc gcggaagcgc cgagaccaac
     3181 cccttcctcg gggtcagggg catccgcttc tccctcgcca accgggagat gttccgcgac
     3241 cagctgaggg ccatgctccg ggcgggggag ggcgccgacc ttgggatcat gcttcccatg
     3301 gtgtctgggg tcgaggaggt cctcgaggcg aaacaggaga tcgccctcgc cgccgccgcc
     3361 ctcgccgagc ggcgggtggc ccacaattcg gcgccgcgca tcggggccat gatcgagctc
     3421 ccctcggccg ccatggccgt gggcgacatc gccggcgaga ccgacttcct ttccatcggc
     3481 tccaacgacc tcaccatgta tctcctggcg gtggaccgca ccaacgagaa gctccaccac
     3541 ctctaccgga cccaccaccc ggcggtgctg cgcgtcttct cggcgatcgc ccgctactcg
     3601 gggagcaagc gagcagagct ctcggtctgc ggggacgtcg cctcggaccc ggtgctcgct
     3661 cccttcttcg tcgggatcgg catcaggaag ctgagcgtca gccccgcgaa ggtggaggcg
     3721 gtgaagcggc gcctcgcctc attcaccctc ggcgagatgc aggccatatc gcgcgagctc
     3781 ctcgccatac ggcgtctcgc cgacatggag cgctatctcg ccgacttcga gggcaggcat
     3841 ccggggatcc cagcggaggc ttcaggcctg tcagtggcga ttggctgagg ccgtgagtac
     3901 aggcaccgag ggggaagccg ccggcaacat ggcgtcgatc tgggggaggg ggtggccgac
     3961 cggccggcgc cggctggctc agaacgaggt tttgcccctc gagggaggcc tgcggccggg
     4021 cgccgggacg cgcagctcgg gccttatgtt ggcgctgggc tctccgaccg ggttctcccg
     4081 cgcgcgccgc ttggacttgt actccttgag agccgtcctg acggcggcca cgatgatggg
     4141 ggtgccgttg tccttgagga ggtagccgta ggaggggatc ctctcggtga gggcggtgaa
     4201 ctcgtcctcg gagtgcgagg agaggaagac tatggggatg tcgaagtcgg cgaggatctg
     4261 cttcgccgtc tgggccccgt ccagacccct gcccaggtcc aggtccatga ggatgaggtc
     4321 gctcggggcc atccccatcc ggagcgcctc gatcgccccc tcgcccgagt aggcgttgtt
     4381 cacgtcgaag ccgtggtcct ggagctccac ggcaagaccc atcgcgagta tcgggtcgtc
     4441 ttcgatgagg aggatgctgg tcggccggtt atacattcct tgcctccgtt gcctcaagcc
     4501 aaaggttact ccaatgtcat gcaaatgcaa ggggcaaatg cgggaaagcc gcgggccccg
     4561 gggctgccta gttcccgatc agaacgctca cccttgtccc gttcccggcc tcgctttcga
     4621 actgtaccct gccctcaagc tgatgcgtga ggagccgaac cagggtgaag cccatgcctt
     4681 cggacaagga ggggtcaagg cccggcggaa ggcccctgcc ctggtccgag acgcggagct
     4741 cgagctcctc gcctcgcctg agtacctcga ccaggatctc gccacgggag ccgggtttgt
     4801 aggcgtgctt cagggagttg gtcagaagct cgttcaggat gagtccgatc gtgaccgccc
     4861 gtttcgggtc cagctccccc gcctcgagcc tggtcctgat cgcgatcatg tcccggtcga
     4921 tgacgtaacc ctcgcagagt gaggcgatga aacgccggaa gtaggcctcg agatcgacgc
     4981 tctcgacgct gtccgaatag gtgagggtct catagaccgc ggccatcgca tggaccctgg
     5041 aacgggcctc gagcagggcg cgatgcgagc tctcgtcgcc cgagacgttg aggctcaggg
     5101 tcagcaggct cgagatgagg ccgaggttgt tcttggtccg gtgctggagt tccttgagga
     5161 ggacatcctt ctcctcgagg gcggcccgta ggctctgctc ggcgcgcctc ttcatgtttc
     5221 tgaaggggga gaggcggggc gctccgcggc agcgctcgtg gacggcgagg gccatcttca
     5281 ccgcggccgc gatcacggga gcgccgttgg tcttgaggat gtagccgtag gacgggacct
     5341 tttcggtgcg ggccgcgaac tcccgctcag agtgggagga gaggaagacg atggggatgt
     5401 cgtggtccct gaggatctcg ctcgcggcct ggacaccgtc gatgccgatc cccagatcga
     5461 gatccatgag gatcagatcg ctcgccgcga tcccctgcct cagggccgcg atggccatct
     5521 ctcccgagta ggcgctgttg accctgaagc cctcgtcctg cagctcgacc gaaagaccca
     5581 tcgcgactat cggatcatcc tccacgagga ggatgctgtg tggctccttg cccattcctc
     5641 cgcctccgac aactcaaggt caaaggtcct ccatatccat gatcatggca aggaaaattt
     5701 acgggaggct cgggcgtggg tggggggtcc ccgactctgc catcactcct ccgagtcgag
     5761 ccaggctacc ttctgctccg gaagatggtc gcgaccgatg atgtcccgat agagctcggg
     5821 cctccgcgcc ctaatgtagc gggagccgcc cgagtcggcg agcttctggt ggacgaggtc
     5881 ggcgacgacg aactggtcat caaaggagcg gcactcggcg aggatgtccc cgaatgggtc
     5941 caggatcatg gacgagccgt tcttgagctg gtcgtcgtcc atgccgatcg ggttcgagaa
     6001 ggccacgaag accccgttgt cgtaggctcg ggccgggagc cacttcatga gccatgagcg
     6061 gcccttgagg ccgtcgaact cggcgcggag gctcgtggga tccttcctcc tgttgtccca
     6121 gagggcccgg tccacgaagc cggcgcccgg cctcggtgag ggggtgcaca tcgtgacgtg
     6181 gggcatgaag atgagctcgg cgccgagcag ggccgtggcg cggacgttct cgatgacgtt
     6241 gttgtcgtag catatgagga tcccgcactt ccagcccttg agctcgaaga ccacatactc
     6301 gttgcccggg ctgaggtgct cgttgatgaa ggggtggagc ttcctgaact tcgccctgag
     6361 cccgctccgg tcgacgcaga cataggcctt gtagagcctg ccctcgtggt ccttctcgaa
     6421 gagcccggcc agtatggcta tcctgtgcag ggacgcgatg cgctggagct cggccacgct
     6481 ccgcccgcct gggatgggct cggccagctc gagcatctgt tcccgcgaga gcctgcgggc
     6541 gaaggaatag gccgtgatcg agcactcgtg gaaggcgatg gcgtcggccc ccgccttcgc
     6601 tgcctcggcc gagagcctct cgatcaccga gaggttgtac cccttgtccc cgttcctgtg
     6661 ctcgaactgg gcgacggcga tcctcaagtc gttcatttcc gccctcctcc tggtccgctg
     6721 ccggccacgc gagtatataa tgcgccagcc ggcggccgcc acgcgccccg ccgtaccgca
     6781 ggaggagcag ccatggacat caaggacatc cccttccagg tcttcgacct ggccaacgcc
     6841 gccccggagg agcacgccgg cgagaccggc agggccgtct ggcggaccgt caagcgcggc
     6901 gagctccgca tccgcatcgt cgaatactcg gccggctacc tcgccgacca ctggtgcgag
     6961 aaggggcacg cggtctatgt ccttgagggg gagttcgaat cggagctcaa ggacgggagg
     7021 aggcataccc tcggcccggg catgtgctac cttgtagccg accacgccga gccccaccgc
     7081 tcctatacta ggaacggggt gaagctcctc atcgtggact agccaggctc cggaggcccc
     7141 gggccgggtt ctggacccgc atgaaatgcc cggtgggatg ggcgtgcagc tgctcgatgg
     7201 cgacgcggac cacgttcttc aggatccact ggtgctcgcc gggactcaag gccccgctcc
     7261 atcgtgcctc ggtgaggacc tccatcagtt tggagatcgt ggcgctgccc ctcatgtcga
     7321 tcgtcccgtt catggcaagc tcctcatggt ggttcgatct tgggatggat gcgagccgtc
     7381 gctgcaatcg gcgttcatgc gaagattccg ccgatatccg tacacagtca agaaaaaacc
     7441 gaggtccggc ccgcccgggc ccaagccggg gtcccgcaac gcgccccggc gggccggtcc
     7501 cgaggctcag ggggagccgg tctggaccga gcggagtatg ctctgcagat cgccgaggcg
     7561 gaccggcttc tcgaggaagg cgaggggctc gagggccctc gccctttcgc gggtgccctt
     7621 gtcctggtat ccggtcatga agatgatcgg gatgtcggcc gactcccgta tcctctgcgc
     7681 ggcctcgatg ccgtcga
//
LOCUS       DASSQV010000289         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_442356, whole genome shotgun sequence.
ACCESSION   DASSQV010000289 DASSQV010000000
VERSION     DASSQV010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442356"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1287)
                     /locus_tag="VFL04_05035"
     CDS             complement(55..1287)
                     /locus_tag="VFL04_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7839101.1"
                     /translation="MKKTDRELGRSAAIAFIVLFGVVSLFADMNYEGGRSVVGQYIKL
                     LGTSALALGFAAGLGEFVGYALRFISGTIADRTRKYWTLIILGYVIQLCSLPLMAFVR
                     GWELAIAFLFLERVGKAIRKPASDAVVSFAAAHTGTGFGFGLQEALDQVGAFLGPSLF
                     ALLLFTAPDGASIAEYRKGLLLLLIPAALAVGITVAARFIFPHPDRFEMKHSAPDIAS
                     RGYSRTYWIIAISAGLLAMGITDFPLIGLHLKKQGIFSEAAIPLLYSGAMAVDAAAAL
                     VFGRLYDRFGLRTLLALFVAQLFTAPLVFLGPTWAIVLGRALWGISVGTQESILKSAI
                     SDRVAKEKRAQAFGLFNTVFGLFWFVGSAAIGFLYDRGGPLPMVIFAVAVQALALGLF
                     STLIFARGKADGGAAKGA"
     gene            1446..>2124
                     /locus_tag="VFL04_05040"
     CDS             1446..>2124
                     /locus_tag="VFL04_05040"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HET7839102.1"
                     /translation="MDPRSDAEAIFRAAVARVDPAPMVAAAVSLARGPVGPVIRATAG
                     GETHEFPLAEGGRVFAVGMGKASARMALGLEAALGDVIFGGIIAVKRGHGEKLPRLRV
                     VEAGHPTPDADSERAARLLLNLGSELGTRLTERDLVVVLVSGGGSAILCAPAEGLSLG
                     DKVEATRLLLGSGASIGEMNRVRKHLSAVKGGRLAEALAPARILSLVLSDVMGDELED
                     IASGPTVP"
BASE COUNT          350 a          680 c          808 g          286 t
ORIGIN      
        1 ctcatggctc gtgaaggggg tcttctaggg tggtcaggct cggcggcgac tgagctaggc
       61 gcccttcgcg gcccccccgt ccgccttccc ccgggcgaag atgagggtcg agaaaaggcc
      121 gagggcgagg gcctggacgg ccacggcgaa gatcaccatg ggcagggggc cgccgcggtc
      181 gtagaggaag ccgatggcgg cgctgccgac gaaccagaag aggccgaaga cggtgttgaa
      241 gaggccgaag gcctgggccc gtttctcctt ggccacgcgg tcgctgatcg ccgacttgag
      301 gatggactcc tgggtgccca cgctgatgcc ccagagcgcc ctcccgagga cgatggccca
      361 ggtcggcccg aggaagacca ggggcgcggt gaagagctgg gccacgaaga gcgcgagcag
      421 ggtccgaagg ccaaagcggt cgtagagccg cccgaagacc agggcggctg cagcgtcgac
      481 ggccatggcc ccagaataca ggagcggtat cgccgcctcg gagaagatcc cctgtttctt
      541 gaggtgcagg ccgatgaggg ggaagtcggt gatgcccatg gccaggagcc ccgctgagat
      601 ggcgatgatc cagtaggtcc gcgagtagcc gcgcgaggcg atgtcggggg cgctgtgctt
      661 catctcgaag cggtcggggt gggggaagat gaaacgcgcg gcgaccgtga tgcccacggc
      721 gagggcggca gggatgagga ggaggaggag gcccttgcgg tactcagcga tgctcgcccc
      781 gtcgggcgct gtgaagagga ggagggcgaa gagcgagggg ccgaggaagg cgccgacctg
      841 gtccagggcc tcctggaggc cgaagccgaa acccgtcccc gtgtgcgccg cggcgaagga
      901 gacgacggcg tcggaggcgg gcttgcggat cgccttgccc acccgctcaa ggaagaggaa
      961 ggctatggcg agctcccagc cgcgcacgaa ggccatgagg ggcagggagc agagctggat
     1021 gacgtaaccg aggatgatga gggtccagta cttcctggtt cggtcggcta tcgtccccga
     1081 gatgaacctg agggcgtagc cgacgaactc gccgagccca gccgcgaagc cgagggcgag
     1141 ggcgctcgtc cccaggagct tgatgtactg gccgacgacg ctccgtccgc cctcgtagtt
     1201 catgtcggcg aaaaggctca cgacgccgaa gaggacgatg aaggcaatgg ccgcgctcct
     1261 gccgagttcc ctgtcggttt tcttcaagcc ccacctcccc gtcgagtatc ccaagatact
     1321 cccggaaggg gggcggcgca aaccgcgcgc gggagtggac gccgaggccc cgcatgctgt
     1381 actacgggag ggcggcgcgg cctccgatgg ccgaggccgc tgagccttgg ccagggagga
     1441 tgggcgtgga tccacgaagc gatgctgagg cgatcttccg agcggccgtg gcccgcgtcg
     1501 accccgcccc catggtggcc gccgcggtct cgctcgcgcg cggccccgtc gggcccgtca
     1561 tcagggccac agccgggggc gagacgcatg agttccccct tgccgagggg ggcagggtct
     1621 tcgcggtcgg catgggcaag gcctcggcca ggatggccct cgggctcgag gccgcgttgg
     1681 gcgatgtcat ctttggaggc atcatcgcgg tgaagcgcgg ccacggcgag aagctccccc
     1741 ggctccgggt cgtcgaggcc gggcacccca cgcccgacgc cgattccgag cgggcggcgc
     1801 ggcttctgct gaacctcggg agcgagctcg gcacccggct caccgagcgc gacctcgtcg
     1861 tggtcctcgt ctccggcgga ggctcggcca tactctgcgc cccggccgag ggcctaagcc
     1921 tcggcgacaa ggtcgaggcg acccgcctcc tcctcggctc gggcgcgtcc atcggcgaga
     1981 tgaaccgcgt gcggaagcac ctctcggcgg tgaagggcgg ccgcctcgcc gaggccctgg
     2041 ccccggcccg gatcctcagc ctcgtgctgt cggacgttat gggcgacgag ctcgaggaca
     2101 tcgcctcggg gccgacggtg cccg
//
LOCUS       DASSQV010000290         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2182779, whole genome shotgun sequence.
ACCESSION   DASSQV010000290 DASSQV010000000
VERSION     DASSQV010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2182779"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2930
                     /locus_tag="VFL04_05045"
     CDS             <1..>2930
                     /locus_tag="VFL04_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HET7839103.1"
                     /translation="SNDLVIAIARDGRGSLWVGTLNGLNRLDEATGSFTRFFNDPKDP
                     HSIPNNTIRALFKDSRGGFWVGTTGGGLASFDYERNRFDNHTAAGAKAGSGVPPSLSI
                     QSITEDGKGALWLGAWGTGIVRFDPRDGSSTTYPLPDNRIYAVNTQEAGMVRVGTWGG
                     GLHVLDVATGAVSSYRNSKAAGTLPSDVVYALMQDASGELWVGTNGGGLARLDRTRRS
                     FQAYVSDSGDPDSLPNGKVIASRVDSRGRLWASIYSNGVHVLDEATGRWRHFRHSAKD
                     PNSLPDDTCNYIYEDSRGELWFATNDGLARYDGASGRFVSYRHREGDPDSLGSSIVYC
                     LVEDRAMPGNFWVGTYTTGLDYWDRGTGKFSHHAFDPADPSSISDNLVNDLAYDGRGR
                     LWVGTNNGLDRLEGGRFVRYHYSADRKDGISSNAVQRLLVDSHQVLWITTRGGGLNRY
                     HPATDGFSHYMRRDGLPNNIAYSVLEDRAGNLWIVTQTGIALFDRESETIKRVSLFKE
                     LANASFNTGSCVGPHGELYFGSVGMVAKFDPLRYETNRHIPPVFVTELRAANRVRYAV
                     PTAAAPAEPIGLATWENSVELFFAALDYRDPLANQFAYKLEGFDRDWTYSTRNFATYT
                     NLPGGSYTFRVKAANNDGLWNVEGAAFPFTVATSPFLSVPAIILYLLAIALLGYGVAK
                     IRSSHALALKVKELTRVQVALEAASQESSRLAAEAGLANRAKSEFIATMSHEIRTPMS
                     GVIGMAELLSRTPIDPVQREYVETIRSSGRLLLGIINNILDFSKLEAKRVVLEDIDFD
                     LDEVLESVLASFAYAASSKSVALEARIGEDVPRRLRGDPLRLGQVLSNLVGNAIKFTD
                     SGSVRVLIELAAMTEEGASLGFRVVDTGIGIKAEHLPKLFNPFTQEDQSTTRRYGGTG
                     LGLSISKQYVELMGGALEVQSAPGSGSTFGFTVTLALAAPKTVPGGAGAGLPAPLIPR
                     VSVL"
BASE COUNT          503 a         1084 c          902 g          441 t
ORIGIN      
        1 tctcgaacga cctggtcatc gccatcgccc gcgacggccg gggctcgctc tgggtgggga
       61 ccctcaacgg cctcaaccgc ctcgacgagg ccacgggcag cttcacccgc ttcttcaacg
      121 acccgaagga tccccactcc atcccgaaca acaccatccg cgccctcttc aaggattcaa
      181 gggggggctt ctgggtgggg acgacgggcg gaggcctcgc ctccttcgac tatgagcgga
      241 accgtttcga caaccacacc gcggccggag ccaaggccgg ctccggggtc cccccctcgc
      301 tctccatcca gagcataacc gaggacggga agggggcact ctggctcggc gcctggggca
      361 ccggtatcgt gcggtttgac ccccgggacg ggagctccac cacctacccc ctgcctgaca
      421 acaggatcta tgccgtgaac acccaggagg ccgggatggt ccgcgtcggg acctggggag
      481 gcgggctcca cgtcctcgac gtggccacgg gagccgtcag ctcctaccgc aactcgaaag
      541 ccgccggcac ccttccgagc gatgtcgtct acgccctcat gcaggacgcc tcgggggagc
      601 tgtgggtggg gaccaacggg ggtggcctcg ccaggctcga caggacgagg cggagcttcc
      661 aggcctacgt atccgattcg ggggatcccg attccctgcc caacggcaag gtgatcgcgt
      721 ccagggtcga ctcccggggc aggctttggg cctcgatcta ctcgaacggg gtccacgtcc
      781 tggacgaggc cacggggaga tggaggcact tccgccactc cgccaaagac ccgaacagcc
      841 tccccgacga cacctgcaac tacatctatg aggactcgag gggagagctg tggttcgcga
      901 cgaacgacgg cctcgcccgg tacgacggcg ccagcggccg cttcgtcagc taccgccacc
      961 gcgaggggga tccggactcg ctgggctcga gcatcgtcta ctgcctcgtc gaggacaggg
     1021 ccatgccggg caacttctgg gtcgggacct acaccacggg cctcgactac tgggaccgcg
     1081 gcacgggcaa gttcagccat cacgccttcg atcccgccga cccctcctcg atctccgaca
     1141 acttggtcaa cgacctggcc tacgacggcc ggggaaggct ctgggtgggc acgaacaacg
     1201 gcctggaccg tctcgagggc ggccgcttcg tccgctacca ctacagcgcc gacaggaagg
     1261 acgggatctc gagcaacgcc gtccagcgcc tcctcgtgga ctcgcatcag gtgctctgga
     1321 tcacgacccg gggcggcggc ctcaaccgct accatccagc gaccgacggc ttcagccact
     1381 acatgcgcag ggacggcctg cccaacaaca tcgcctacag cgtcctcgag gaccgcgcgg
     1441 ggaatctctg gatcgtcacc cagaccggca tagccctctt cgaccgcgag tccgagacga
     1501 tcaagagggt cagcctcttc aaggagctcg ccaacgcctc cttcaacacg ggctcctgcg
     1561 tcggccccca cggcgagctc tacttcggct cggtgggcat ggtggcgaag ttcgaccccc
     1621 tgaggtacga gaccaaccgc cacatccccc ccgtcttcgt caccgagctc agggcggcca
     1681 accgggtgcg ctatgccgtc ccgaccgccg cggcccccgc cgagcccatc ggcctcgcga
     1741 cctgggagaa ctccgtggag ctcttcttcg cggccctcga ctaccgcgac cccctcgcca
     1801 accagttcgc ctacaagctc gagggcttcg acagggactg gacctactcc acgcgcaact
     1861 tcgcgaccta cacgaacctg ccggggggct cctacacctt ccgcgtcaag gccgccaaca
     1921 acgacgggct ctggaacgtg gagggagccg ccttcccctt cacggtggcg acctcgccct
     1981 tcctgagcgt ccccgccatc atcctctacc tcctcgcgat cgccctcctg ggctacggcg
     2041 tcgcgaagat caggtcgagc cacgccctgg ccctcaaggt gaaggagctc accagggtcc
     2101 aggtggccct cgaggccgcc agccaggagt cgagcaggct cgcggccgag gcgggcctcg
     2161 ccaaccgcgc caagagcgag ttcatcgcga cgatgagcca cgagatcagg acgccgatga
     2221 gcggggtgat cggcatggcg gagctcctgt cgcgcacgcc gatcgacccc gtccagaggg
     2281 aatacgtcga gaccatccgc tccagcggcc gcctcctcct cggcatcatc aacaacatcc
     2341 tcgacttctc gaagctcgag gccaaacgcg tcgtcctcga ggacatcgac ttcgacctgg
     2401 acgaggtcct ggaatcggtg ctcgcctcct tcgcctacgc ggcctcctcc aagtcggtcg
     2461 ccctcgaggc gaggatcggc gaggatgtgc cccggaggct ccgcggcgac cccctccgcc
     2521 tcggccaggt cctctcgaac ctcgtcggca acgccatcaa gttcaccgac agcggatcgg
     2581 tgcgggtcct catcgagctg gcggcgatga cggaggaggg ggcttccctc ggcttcaggg
     2641 tcgtcgacac cgggataggc atcaaggccg agcacctccc caagctcttc aaccccttca
     2701 cccaagagga ccagtcgacg acccggcgct acggcgggac tgggctcggg ctctcgatct
     2761 ccaagcagta cgtcgagctc atgggcgggg ccctcgaggt ccagagcgcg ccgggctcgg
     2821 gctcgacctt cggcttcacg gtcaccctgg cccttgccgc gccgaaaacc gtcccgggcg
     2881 gagccggagc gggccttccc gcgcccctga tccctcgagt ctcggtcctt
//
LOCUS       DASSQV010000291         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_317209, whole genome shotgun sequence.
ACCESSION   DASSQV010000291 DASSQV010000000
VERSION     DASSQV010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317209"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFL04_05050"
     CDS             complement(<1..756)
                     /locus_tag="VFL04_05050"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET7839104.1"
                     /translation="MKKLIFVTGGVCSSLGKGVAASSIGALMEARGLSVRMVKIDPYL
                     NVDAGTMSPYQHGEVYVTDDGAETDLDLGNYGRYTNSPLSKANSITTGQVYQAVINKE
                     REGRFLGRTVQVIPHITDEIKNRVLAPGKDPEVDITIIEIGGTVGDMESIPFLEAARQ
                     LINEMGRQNAISVHVTLVPAVSGGELKTKPTQHAVKELQEVGIQPDVLICRGTLPLEE
                     GLRRKIAGFTNVDYEAVVSAHDVKTTIYEIPLVF"
     gene            845..1291
                     /locus_tag="VFL04_05055"
     CDS             845..1291
                     /locus_tag="VFL04_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839105.1"
                     /translation="MEYGVARQVVKDEVFYTLRWSPFVKADKYDIVTRVPAIGGIAEL
                     YYMDEKGKLNLFCLQRSWYGGLRSMLRERCDPSLEKDPFRLSILVKWEGRIYHRWTNC
                     ESAADMSDIMFFFSETYAPGSGSVRSSGRYHRIFLNELDTGKLVTI"
     gene            1347..>2083
                     /locus_tag="VFL04_05060"
     CDS             1347..>2083
                     /locus_tag="VFL04_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HET7839106.1"
                     /translation="MIRGLYTGASGMSAQQVRLDAIANNLANVDTDGYKRDVAVHKAF
                     AQLLLRRMNDDGVQTGPFGSSDVSPVIGKVGTGVENNELFTELEQGALKETQSDFDVA
                     LDGPGFIAIQTPYGERYTRNGSFVLGKEGFLETKDGYPVLGENGPIKVKENNFTIDAK
                     GGIFINKTFQDDPFRLVSKDENTWDKLQKLDNLKVVDFQKPRFLAKQGGSLWASTEES
                     GDAKLLAQESRPKVIQGFAEASNVNPVL"
BASE COUNT          381 a          688 c          655 g          359 t
ORIGIN      
        1 gaacacgagg gggatctcgt agatggtcgt cttcacgtcg tgggccgaga ccacggcctc
       61 gtagtcgacg ttggtgaagc cggcgatctt gcgtctgagg ccctcctcga gggggagggt
      121 gccgcggcag atgaggacgt cgggctggat cccgacctcc tggagctcct tgaccgcgtg
      181 ctgggtgggc ttggtcttga gttccccgcc cgagaccgcc gggaccaggg tcacgtggac
      241 cgagatcgcg ttctgccggc ccatctcgtt gatgagctgg cgggcggcct cgaggaaggg
      301 gatggactcc atgtcgccga cggtcccgcc gatctcgatg atcgtgatgt cgacctcggg
      361 gtccttgccc ggggccagca cgcggttctt gatctcgtcg gtgatgtggg ggatgacctg
      421 gaccgtccgg cccaggaagc gcccttccct ctccttgttg atcaccgcct ggtagacctg
      481 gcccgtggtg atcgagttgg ccttggagag gggggaattg gtgtagcgtc cgtagttgcc
      541 gagatcgagg tcggtttcgg caccgtcgtc ggtgacgtag acctcgccgt gctggtaggg
      601 ggacatagtc cccgcatcca cgttcaggta ggggtcgatc ttgaccatgc gcacggacag
      661 gccgcgcgcc tccatgagag cgccgatcga ggaggccgcc acgcccttgc caagggagga
      721 gcagacgccg cccgtcacga agataagctt cttcatgggg ttttagtatc cccatcgcgc
      781 ccgacactgt caatcgcgcc ggaacggcct ttcatgaagg ccccgcccgt gctatcgtcg
      841 gggcgtggaa tacggggtcg cccgccaggt cgtcaaggac gaggtcttct ataccctccg
      901 ctggtctccc ttcgtcaagg ccgacaagta cgacatcgtg accagggtcc ccgccatcgg
      961 cggcatcgcc gagctctact acatggacga gaagggcaaa ctcaacctct tctgcctcca
     1021 gcgctcatgg tacgggggcc tccgctcgat gctcagggaa cgctgcgacc cctccctcga
     1081 aaaggacccc ttcaggctct ccatcctcgt gaagtgggag ggccggatct accaccgctg
     1141 gaccaactgc gagtcggccg ccgacatgag cgacatcatg ttcttcttca gcgagaccta
     1201 cgcccccggc tcgggttcgg tccgctcctc gggccgctac cacaggatct tcctcaacga
     1261 gctcgacacc ggaaaactgg tgaccatcta gcggtaaact ccggcccccc gcgtaccgat
     1321 catcgataca tgaggggata ccaggcgtga tccgcggtct gtatacgggt gcgagcggca
     1381 tgtccgccca gcaggtgagg ctcgacgcca tcgccaacaa cctcgcgaac gtcgacaccg
     1441 acggctacaa aagggacgtg gcggtccaca aggccttcgc ccagctcctg ctcaggcgga
     1501 tgaacgacga cggggtccag acgggcccct tcggctcgag cgacgtctcg ccggtgatcg
     1561 gcaaggtcgg gaccggggtc gagaacaacg agctcttcac cgagctcgag cagggagccc
     1621 tcaaggagac ccagagcgac ttcgacgtgg ccctcgacgg cccgggcttc atcgccatcc
     1681 agacccccta tggcgagcgc tacacccgca acggctcctt cgtcctcggc aaggagggct
     1741 tcctcgagac caaggacggc taccccgtcc tcggcgagaa cggccccatc aaggtgaagg
     1801 agaacaactt caccatcgac gccaagggcg gcatcttcat caacaagacc ttccaggacg
     1861 accccttccg cctcgtctct aaggacgaga acacctggga caagctccag aagctcgaca
     1921 acctcaaggt ggtcgacttc cagaagccgc gcttcctcgc caagcagggg gggagcctct
     1981 gggcctcgac cgaggagtcc ggggacgcga agctcctcgc ccaggaatcg cggcccaagg
     2041 tgatccaggg cttcgccgaa gcctccaacg tgaaccccgt cct
//
LOCUS       DASSQV010000292         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_690291, whole genome shotgun sequence.
ACCESSION   DASSQV010000292 DASSQV010000000
VERSION     DASSQV010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690291"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /locus_tag="VFL04_05065"
     CDS             complement(<1..1620)
                     /locus_tag="VFL04_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839107.1"
                     /translation="MRLDTAERIAHLIAETGRIEHESEGLYLTLGRLFPKLSDEMAKS
                     AKNAEASLRNFDTLSSGDGTGSLRAMCSFAEGSGEFFESVHSRDSAFLTQINESIDRL
                     GSLEGIIARVRSDSEEMEIISLNAMTVALKSGAAGKAFSVITDELKRLSGRTIALTED
                     VTERGRSLLEQFHRLQASVAELDAFQADFFKGIQKEMTEGFSEIEGSVNNAASFFRGL
                     LEKARGVREPVTRVMQEVQLQDIVRQSLQHVVLSLQEAKSELAPDAPESIGGLEVEDE
                     TPERADAANLAFVGAVAELSAGLIEDIMGKLDASIQSFAADMDVVRAVVGDCERQRSD
                     FLVGSGASCEPMDTGAYTTGSLRYLSLKRAVIAMARRLSDQVKGLDASFKGLAQILSR
                     FQTIVVASRIEVAKTTALGGVATTVGGMISLTDRIGVDVGEAMGTTKDFIRRASDAIG
                     SYAAREGGEGDELTATLSKVESDMACLDQARVSVCAAIDGFSLYTKEFIGLVMEVRKS
                     LDGLRALSARLVEARTELLSLLEYIRGLPGGI"
     gene            complement(1763..>2732)
                     /locus_tag="VFL04_05070"
     CDS             complement(1763..>2732)
                     /locus_tag="VFL04_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7839108.1"
                     /translation="GLEDITEGELLIDSKLVNDVPPKDRDIAMVFQNYALYPHMTVFE
                     NMAFGLRIRKLPKDEIEKRVKEAARILDIEKLLDRRPKQLSGGQRQRVAVGRAIVRNP
                     KVFLFDEPLSNLDATLRVQMRAELIDLHYRLDATMIYVTHDQVEAMTMGDKIVVMRDG
                     KIQQIGSPLYLYNQPINKFVAGFLGTPPMNFITVKVIEEGGRVLVDEGSFKLAVAPEH
                     QALLKSHVGKEVSFGIRPEDLECVPTLTPAAALKAKVTVVEPLGADINLYATTSKSAM
                     TARVPPHHLFKMGDEVILAPVMAKARYFDKDTELSILPVKWDEQAS"
BASE COUNT          423 a          841 c          981 g          487 t
ORIGIN      
        1 gatccccccg ggcaggcccc tgatgtactc gaggagggag aggagctcgg tgcgcgcctc
       61 gacgagccgg gccgacaggg cccgcagccc gtccagggac ttgcggacct ccatgacgag
      121 gccgatgaac tccttcgtgt agagcgagaa gccgtctatg gccgcgcaga ccgagaccct
      181 ggcctggtcc agacaggcca tgtccgactc gaccttggat agggtggcgg tgagctcgtc
      241 gccctcccct ccttcgcggg ctgcgtagct gccgatggcg tcgctcgccc tcctgatgaa
      301 gtccttggtc gtccccatgg cctcgcccac gtcgaccccg atccggtcgg tgagtgagat
      361 catgccgccc acggtggtag ccaccccgcc gagggccgtg gtcttggcca cctcgatcct
      421 cgaggccacc acgatggtct ggaaacgcga gaggatctgg gccaggccct tgaagctcgc
      481 gtcgaggccc ttgacctggt cggagagccg gcgcgccatg gctatgaccg cccgtttgag
      541 gctgaggtag cggagcgagc ccgtggtgta ggctcccgtg tccatcggct cgcaggaggc
      601 gcccgagccg acgaggaagt ccgacctctg ccgctcgcag tcgccgacga cggcgcggac
      661 cacgtccatg tcggcggcga agctctggat gctcgcgtcg agcttgccca tgatgtcctc
      721 gatgaggccg gccgaaagct cggcgacggc cccaacgaag gcgaggttgg cggcgtcggc
      781 cctttcgggc gtctcgtcct cgacctcgag ccccccgatc gactcgggcg catcgggggc
      841 gagctcgctc ttcgcctcct gcagggagag gacgacgtgc tggagggact ggcggacgat
      901 gtcctggagc tggacctcct gcatgaccct ggtgaccggc tcgcggaccc cgcgggcctt
      961 ttcgaggagg ccgcggaaga aggaggcagc gttgttcacg ctgccctcga tctccgagaa
     1021 gccctcggtc atctccttct ggatgccctt gaagaaatcg gcctggaagg cgtcgagctc
     1081 ggccaccgag gcctggagcc tgtggaactg ctcgagcagc gagcgcccgc gctcggtgac
     1141 gtcctcggtg agggcaatgg tcctccctga cagacgcttg agctcgtcgg tgatgaccga
     1201 gaaggccttg cccgccgcgc cggacttgag ggcgaccgtc atcgcgttga gcgagatgat
     1261 ctccatctcc tcggagtccg agcgcacccg ggcgatgatg ccctcgagcg agccgaggcg
     1321 gtcgatgctc tcgttgatct gggtgaggaa ggccgagtcc ctggagtgca ccgactcgaa
     1381 gaactcgccc gagccctcgg caaaggagca catcgccctg aggctgcccg tgccgtcgcc
     1441 cgagctgagg gtgtcgaagt tgcgcaggga ggcctcggcg ttcttcgcgc tcttcgccat
     1501 ctcgtcggag agcttgggga agaggcgtcc gagcgtaagg tagaggccct cgctttcgtg
     1561 ttcgatgcgg cccgtctctg ctatcaggtg ggcaatccgc tcggctgtgt caaggcgcat
     1621 tgcatatcac tatacgatat gcaggttccg gtcgcaagga cagcggcgcc ctgcgccgcc
     1681 tcatcgtggc cgcgagggca gggtgcgggc gaaaaaaagg cgcccggaag ccgggcgcct
     1741 gcgtctggcc gggattccgg ccctagctcg cctgctcgtc ccacttcacg gggaggatgg
     1801 agagctcggt gtccttgtcg aagtaccggg ccttggccat gacgggggcg aggatgacct
     1861 cgtcgcccat cttgaagagg tggtggggcg gcacacgggc ggtcatggcg ctcttggagg
     1921 tggtcgcgta gaggttgatg tccgcgccga ggggctccac gaccgtgacc ttggccttga
     1981 gggccgcggc cggggtgagg gtcgggacgc actcgaggtc ctcgggcctg atgccgaagg
     2041 agacttcctt gccgacgtgg gacttgagga gggcctggtg ctcgggggcg acggcgagct
     2101 tgaaggagcc ctcgtccacg aggacgcggc cgccttcctc gatcaccttc accgtgatga
     2161 agttcatggg cggcgtgccg aggaagccgg cgacgaactt gttgatcggc tggttgtaca
     2221 ggtacagcgg ggagccgatc tgctggatct tgccgtcgcg catgacgacg atcttgtcgc
     2281 ccatggtcat ggcctcgacc tggtcgtggg tgacgtagat catcgtggcg tcaaggcgat
     2341 agtggaggtc gatgagctcg gcgcgcatct ggacgcggag cgtggcgtcg aggttggaga
     2401 ggggctcgtc gaagaggaag accttggggt tgcggacgat ggcgcggccg accgcgacgc
     2461 gctggcgctg accgcccgaa agctgcttgg gccggcggtc gaggagcttc tcgatgtcga
     2521 ggatgcgggc ggcttccttg acgcgcttct cgatctcgtc cttggggagc ttgcggatgc
     2581 ggagaccgaa ggccatgttc tcgaacacgg tcatgtgggg atacagagcg tagttctgga
     2641 acaccatcgc gatgtcgcga tccttgggcg gcacgtcgtt gacgagcttg gaatcgatga
     2701 gcagctcgcc ctcggtgatg tcctcgagac cc
//
LOCUS       DASSQV010000293         5597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_194144, whole genome shotgun sequence.
ACCESSION   DASSQV010000293 DASSQV010000000
VERSION     DASSQV010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194144"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1238
                     /locus_tag="VFL04_05075"
     CDS             126..1238
                     /locus_tag="VFL04_05075"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7839109.1"
                     /translation="MSIELPLAQTTNLWHSGPSTGEMGALWGSLAVIGVIVIAALIYR
                     GLKTGSSPLSGPRPARSASGFSRAAFRRAARATGLPEEEVRFLEDYAKALGLGNPEFV
                     FRNQAKLDAFFKDVFRYIEKNSESEAGADELKARLFGARERLTHQHALGGPISSSRQL
                     GRNTPLTFIAPGEESYPSVIVAVEPGGLAVEPARDAYGEAIRFKRSAKLAVFFYAQGH
                     QGYQFPSRVVGWERIGTKETMVIAHSDEVAPLPARRHARKEMRAPCTVYRVTVTVKKA
                     KGRDQSTARVENLPYPGTIVDISAGGIGIQSANPLPAGEFIKIEFDSASGTQAAFCKV
                     LRMSRARIGGIMHAQFVRISRRALNAILSYVYGYAE"
     gene            1277..1573
                     /locus_tag="VFL04_05080"
     CDS             1277..1573
                     /locus_tag="VFL04_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839110.1"
                     /translation="MTLTYRIAYNALMKVVELRELNRRNVAIHYIREFTAIAVLQSKD
                     SQTETEIAFVLESKAVGPPELSVRVLDPVDWPLLPVVRALREHIMELETSGRLN"
     gene            1570..2010
                     /gene="tsaE"
                     /locus_tag="VFL04_05085"
     CDS             1570..2010
                     /gene="tsaE"
                     /locus_tag="VFL04_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET7839111.1"
                     /translation="MREFRLHDTQDTLDLGERIGRAATGGSVIALRGGLGAGKTTLAK
                     GVALGLGIDEEVTSPTYTIVSEYEGRLRLHHIDAYRLSGAEEFLALGGDELLSDSGGI
                     CLVEWSERIESALPPESSVLELRIEADESRTAVLAGAGLEGALA"
     gene            2007..2690
                     /gene="tsaB"
                     /locus_tag="VFL04_05090"
     CDS             2007..2690
                     /gene="tsaB"
                     /locus_tag="VFL04_05090"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET7839112.1"
                     /translation="MKVLAFDCSGEVLSVAVRDGGSWAEASLDFGLRHAERLMDLVDR
                     CLAWAGIRPAELGLIACTIGPGSFTGLRIGISTAKGMALGLGVPWVGVPSLDCLAWGH
                     EANEGPVAPIMDARRGRVYSAIYLRGRRISDWLDLPLAELAALLDTYPQALVTGPDAE
                     LFEPYAADRSGFRIDPRARQSAARALAVLGEARFAAEGGAKEDAGPLYLRPVEADRAG
                     PPPGAGTTE"
     gene            2715..3827
                     /locus_tag="VFL04_05095"
     CDS             2715..3827
                     /locus_tag="VFL04_05095"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET7839113.1"
                     /translation="MDELRLSEEPEELEELEELEAPEDFELLERGVNALPSESPLLTE
                     SGPRSAVRSYARLAEPLALLDDSFGFVYRNPPFSRLLKAFGYPDSSSFLGTLSRAIDK
                     DKAKEIHESLSSEERSHSWKGTVSHKAKNSAAVITKVHIFPLFAEAPRDRSPEAYSVY
                     LDDVTEENRKILRDMFSSLLEASKLKDNDTGRHIERVNLYSSRLATELFNDPRWPEVD
                     QDFIDNLGFLAAMHDVGKIGTPDDILNKVGPLNEFEWKLMKEHTLNGAFILSSYPNPM
                     AKEIALSHHEWWNGSGYPFNLEGRMIPLSARIVTVADVYDALRMKRSYKPAYPHDVAI
                     EKIRQEKGVHFDPGLIDMLARVEADFAEIFAANADS"
     gene            complement(3879..5261)
                     /locus_tag="VFL04_05100"
     CDS             complement(3879..5261)
                     /locus_tag="VFL04_05100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7839114.1"
                     /translation="MLRGLERRRELDPAFLISDAESTLTHLYIQEGNDQEGRGEPAAI
                     ALAATVAAYEHFIAEWKRASPPPSGEGRGSEAKTPERGETMSLEIRRVENLRQLKAFI
                     RFPYRIYGGDPCWVPPLDFDDLGTLRKDKNPAFEHCEAEYWIAYRDGSPVGRIAGIIN
                     HPANEKWGTKLARFGWIDFVEDFEVAEALLRTVEDWARSKGLEGVHGPLGFTDFDREG
                     MLVEGFAELATLATNYNRPYYPQYLERLGYAKDIDWVEFQIATPSVIPEKVLRVQELI
                     AKRSGIHLHEWKDKKELIGKWADDLFEILEEAYAHLYGTAPLTKRQVQAYIKTYLGFV
                     DPRFTKIVVDKDGKLIGFGITMPSLSKALQKSRGRLLPFGWLHLMLALRKPTVIDMYL
                     IAVRPEYQSRGVVAFLMTSLNQAAIDAGVKVAESNPELETNVEVQSLWKGYEKRQHKR
                     RRIYLKRLAP"
BASE COUNT          927 a         1895 c         1870 g          905 t
ORIGIN      
        1 ctccgcgagc tcaccggggc cttcgagaac aaggacgccg tcctcatcgg cgacctcgcc
       61 gaatacgagg tcctgccccg gatgaagagc ttcttcgggg ccatggagga ggcgctgcca
      121 tcggcatgag catcgagctg cccctcgccc agaccaccaa cctctggcac tcgggcccct
      181 cgacgggcga gatgggcgcc ctctggggca gcctcgccgt catcggcgtc atcgtcatcg
      241 ccgccctgat ctaccgcggg ctcaagaccg gctcctcccc cttgagcggc ccgaggccgg
      301 cgcggagcgc ctcgggcttc agccgggcgg ccttccgccg ggcggcccgt gccaccggcc
      361 tgcccgagga ggaggtgcgc ttcctcgagg actacgccaa ggccctcggc ctcggcaacc
      421 ccgagttcgt cttccgcaac caggcgaagc tcgacgcctt cttcaaggac gtgttcaggt
      481 acatcgagaa gaactccgag tcggaggccg gggccgatga gctcaaggcg aggctcttcg
      541 gggccaggga gaggctcacc caccagcacg ccctcggcgg gcccatctcc tcgtcgcgcc
      601 agctcgggag gaacacgccc ctgaccttca tcgcccccgg cgaggagtcc tacccctcgg
      661 tgatcgtcgc cgtcgagccc ggcggcctcg ccgtcgagcc ggcgcgcgac gcctacggcg
      721 aggcgatccg cttcaagcgg agcgccaagc tggcggtctt cttctacgcc cagggccacc
      781 agggctacca gttcccctcc cgcgtcgtcg gctgggagcg gatcgggacg aaggagacca
      841 tggtgatcgc ccacagcgac gaggtcgccc cgcttcccgc gcgcagacac gcgcggaagg
      901 agatgcgggc gccctgcacc gtctatcgcg tcaccgtgac cgtgaagaag gcgaagggca
      961 gggaccagtc gacggcgagg gtggagaacc tcccctaccc cgggaccatc gtggacatct
     1021 cggccggagg catcggcatc cagagcgcga acccccttcc tgcgggagag ttcatcaaga
     1081 tcgagttcga ctcggcgagc ggaacccagg cggccttctg caaagtgctc aggatgagcc
     1141 gcgccaggat cggcgggatc atgcacgccc agttcgtcag gatctcgcgc cgcgcgctca
     1201 acgccatcct ctcttacgtc tacggctacg cggagtgagg agcttccccg ctgtctctgt
     1261 gcgcgggggg cgcaacttga cgctcacgta ccgcattgcc tacaatgcac ttatgaaggt
     1321 cgtcgagctc agggagctca atcgaagaaa cgttgcgatc cattacatca gggagttcac
     1381 ggccatcgcg gtccttcagt cgaaggacag ccagacggag acggagatag ccttcgtcct
     1441 ggagtccaag gcggtcggcc cacccgagct cagcgtcagg gtcctcgacc ccgtcgactg
     1501 gccccttctg cccgtggtgc gcgccctgcg cgaacacatc atggagctcg agacctcggg
     1561 gagactcaac tgagggagtt ccgcctccac gacacccaag acaccctcga cctcggcgag
     1621 cgcatcggca gggccgccac cggcggctcg gtgatcgccc tcaggggggg gctcggcgca
     1681 ggcaagacca ccctggccaa gggagtggcc ctcggcctcg ggatcgacga ggaggtcacc
     1741 agccccacct acaccatcgt ctcggagtat gaggggcggc tcaggctcca ccacatagac
     1801 gcataccggc tttccggagc cgaggagttc ctcgccctcg gcggagacga gctcctctcg
     1861 gactcaggcg ggatctgcct cgtcgagtgg agcgagagga tcgagtcggc ccttcccccg
     1921 gagagctcgg tcctcgagct caggatcgag gctgacgaga gccggaccgc agtcctggct
     1981 ggggctgggc tcgagggggc cctcgcgtga aggtcctcgc cttcgactgc tcgggcgagg
     2041 tcctctcggt ggccgtccgc gacggcggat cctgggccga ggcctccctg gacttcgggc
     2101 tcagacacgc cgagcgcctc atggacctcg tcgaccgctg cctcgcctgg gcgggcatca
     2161 gaccggccga gctcgggctc atagcctgca cgatcgggcc gggctccttc acggggctca
     2221 ggatcgggat ctccacggcc aagggcatgg cccttggcct cggcgtcccc tgggtgggcg
     2281 tgcccagcct cgactgcctg gcctggggcc atgaggcaaa cgaggggccc gtggccccca
     2341 tcatggacgc gaggcggggc agggtctact cggccatcta tcttcggggc aggaggatca
     2401 gcgactggct cgacctgccc cttgcggagc tcgcggccct gctcgatact tacccgcagg
     2461 ccctcgtcac ggggcccgac gccgagctct tcgagcccta cgccgccgat cggagcggtt
     2521 tccgcatcga cccgagggcc cgccagagcg ccgcccgggc tcttgccgtc ctcggcgaag
     2581 cgcgcttcgc agcagagggg ggagcgaagg aagacgcggg gcccctgtac ctgcggcccg
     2641 tcgaggcgga ccgggcggga cctcccccag gggcaggcac aaccgaatag gcgtcgccgg
     2701 aaagcgcagg tgtcatggac gagcttcggc tttctgaaga gccggaggag cttgaggagc
     2761 tcgaggagct cgaggctccc gaggatttcg agctgctcga gcgcggcgtc aacgcgctgc
     2821 cctcagagtc tcccctcctc accgaaagcg gccccaggag cgcggtccgc agctatgccc
     2881 ggctggccga gcccctcgcc ctcctcgacg actccttcgg cttcgtctac cgcaacccgc
     2941 ccttctcgag gctcctcaag gccttcggct accccgactc ctcgtccttc ctcgggaccc
     3001 tgtcgcgagc gatagacaag gacaaggcga aggagatcca cgaatccctc tcgagcgagg
     3061 agaggagcca cagctggaag gggacggtct cccacaaggc caagaactcg gccgccgtaa
     3121 tcaccaaggt ccacatcttc ccgcttttcg ccgaagcgcc ccgggacagg agccccgagg
     3181 cctactccgt gtacctcgac gacgtgaccg aggagaaccg caagatcctc agggacatgt
     3241 tctcctcgct gctcgaggcc tccaagctca aggacaacga cacgggaagg cacatcgagc
     3301 gggtcaacct ctactcgagc cggctcgcca cggagctctt caacgacccg cgctggcccg
     3361 aggtggacca ggacttcatc gacaacctcg gcttcctcgc ggccatgcac gacgtgggca
     3421 agatcggcac ccccgacgac atcctcaaca aggtggggcc gctcaacgag ttcgaatgga
     3481 agctgatgaa ggagcacacc ctcaacgggg ccttcatcct ctcgagctac cccaacccga
     3541 tggccaagga gatcgccctg tcccaccacg agtggtggaa cggctcgggc tatcccttca
     3601 acctcgaggg gcggatgata cccctgtccg cccggatcgt gaccgtggcc gacgtctacg
     3661 acgccctcag gatgaagcgg agctacaagc ccgcctatcc ccacgacgtc gcgatcgaga
     3721 agatccggca ggagaagggc gtgcatttcg accccggcct catcgacatg ctggccaggg
     3781 tcgaggccga cttcgcggag atcttcgccg ccaacgcgga ttcatgatcg gcggcgcgcc
     3841 acgcgcccac ctgaggaccg tcggcgcgcc gaggcagact agggcgcgag cctcttgagg
     3901 tagatcctgc gccgcttgtg ctgccgcttc tcgtagccct tccagaggct ctggacctcg
     3961 acgttggtct cgagctcggg gttggactcc gccaccttca cccccgcgtc gatcgcggcc
     4021 tggttgaggc tcgtcatgag gaaggccacc acgccgcgcg actggtactc gggcctgacg
     4081 gcgatcaggt acatgtcgat caccgtgggc ttgcggaggg ccagcatgag gtggagccag
     4141 ccgaagggca ggagcctgcc cctggacttc tgcagggcct tggagaggct cggcatggtg
     4201 atgccaaagc cgatcagctt gccgtccttg tccaccacga tcttcgtgaa ccggggatcg
     4261 acgaagccca gataggtctt gatgtaggcc tgcacctgcc gcttggtgag gggggcggtg
     4321 ccgtagaggt gggcgtaggc ctcctcgagg atctcgaaga ggtcgtcggc ccacttcccg
     4381 atgagctcct tcttgtcctt ccactcgtga aggtggatgc ccgagcgctt cgcgatgagc
     4441 tcctgcacgc ggaggacctt ctcggggatg acgctgggcg tcgcgatctg gaactcgacc
     4501 cagtcgatgt ccttggcgta gccgaggcgc tcgaggtact ggggatagta gggcctgttg
     4561 tagttcgtcg ctagggtggc gagctcggcg aagccctcga cgagcatgcc ctcgcggtcg
     4621 aagtcggtga agcccagggg gccgtgcacg ccctcgaggc ccttggagcg ggcccagtcc
     4681 tcgacggtcc ggaggagggc ctcggcgacc tcgaagtcct cgacgaaatc gatccatccg
     4741 aagcgggcga gcttcgtgcc ccacttctcg ttcgcggggt ggttgatgat gcccgcgatg
     4801 cgtccgacgg gggagccgtc gcggtaggcg atccagtatt cggcctcgca gtgctcgaag
     4861 gcggggttct tgtccttgcg gagggtgccc aggtcgtcga agtcgagggg agggacccag
     4921 caggggtcgc cgccgtagat gcggtagggg aagcggatga aggccttgag ctggcgcagg
     4981 ttctcgacgc gtcggatctc gagggacatg gtttctcctc tttcgggagt tttcgcctcg
     5041 gagccgcgcc cctcgcccga gggaggggga gaggcgcgct tccattcggc gatgaagtgc
     5101 tcataggcgg ccacggtggc ggcgagggcg atggcggcgg gctcccccct gccctcctgg
     5161 tcgttgcctt cctggatgta gagatgagta agggtgctct cagcgtcgga gatcaggaag
     5221 gcggggtcga gctcgcgtct gcgctccagc ccacggagca cgctttcgaa gacttcctga
     5281 tgtttctgtt cacgccagcc ggttgtcgac acgctttgag gctcactttc gaggcggaag
     5341 tatacccgcg gatcggggca actgtaaacc gaaatgcccg ggtccctgag ctcttttctt
     5401 gttccggcct gggttgaggg ccctagtggc ggagctcgaa gctcctccgc tcgatgaggc
     5461 tcggcgccag gaaggcgtcg ggcgccgccc cgagctcgag ccctgatagg ggcggactcc
     5521 ggtcgatgag ggtcctgcgc tcggcccagg gcatggcccc cgccggggga tagcggagga
     5581 caagacgcct cgcctcc
//
LOCUS       DASSQV010000294         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_814955, whole genome shotgun sequence.
ACCESSION   DASSQV010000294 DASSQV010000000
VERSION     DASSQV010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814955"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..902)
                     /locus_tag="VFL04_05105"
     CDS             complement(63..902)
                     /locus_tag="VFL04_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET7839115.1"
                     /translation="MKIAVLVKQVPETDSLTLDPETGTVIRSEDSSIVNPLDLYALEA
                     ALRIKDADPATTILALSMGPPQAETALREAMAMGCDEAVLVTGKEFGGSDTWATARAL
                     SAALRSLGPFDLVFCGEKATDGDTGQVGPELAAFLCLPVLSYVGGIRTVTVPTGIELT
                     VERITEEGIQVVRCGAPAVLTFCKAVGEPRLPLLSGKRRAREAGVRRLGIAELGLSAE
                     ETGVKGSPTKVVRIDRPRLSRETEWIDARSPEGIEAACDRIIAILEAKGLIARLPGAG
                     RGA"
     gene            complement(1051..>2087)
                     /gene="nagA"
                     /locus_tag="VFL04_05110"
     CDS             complement(1051..>2087)
                     /gene="nagA"
                     /locus_tag="VFL04_05110"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET7839116.1"
                     /translation="SDALILDVGGAYIAPGLIDTHIHGFAGFGTEDMSVDAIMAMSEH
                     LGGFGVTAFCPTIYPMTEEDMVASIEACVGAMGRETGAKVMGVHLEGPFVSQARLGVQ
                     RPEFVRAVDLELMERLYQAGRGHITNMTVAPELKGMRELALYCIKRGIVLQAGHTDAT
                     YENMLEGMQAGILHSTHFFNAMSRLHHRDPGAVGAIMIHSELSCEIIADGHHVHPDLV
                     RLLMRDKPVSKVVLVTDSLKPTEQHNCPLFANREEVELRDRLFYRKKDDIIAGSSLTM
                     IEGLRNLVDFGVPLENAVEMAASNPSRILGLGKRGLLVPGYEADILVFDRDFRVLATL
                     VGGRFVKDKL"
BASE COUNT          322 a          674 c          741 g          350 t
ORIGIN      
        1 gagctcgaat gaaacagggg ccacctggcc cgccttggtc tcggcgagga tccagacctc
       61 gttcatgccc ccctcccggc gcccggaagc ctggctatga ggcccttagc ctcgaggatg
      121 gcgatgatcc ggtcgcatgc cgcctcgatc ccctcggggc tccgcgcgtc gatccattcg
      181 gtctcgcggg aaagccgggg cctgtcgatc ctgaccacct tggtcggcga gcccttgacc
      241 cccgtctcct cggccgagag cccgagctcg gcgatgccga ggcggcggac gccggcctcc
      301 ctggccctcc gcttgcccga gagcagggga agacggggct cgccgacggc cttgcagaag
      361 gtgaggacgg cgggggctcc gcagcggacg acctggatgc cctcctccgt gatccgctcc
      421 acggtcagct cgatgccggt ggggacagtc accgtcctga tcccgcccac atagctcagg
      481 accgggaggc agaggaaggc cgcgagctcg ggccccacct gtcccgtgtc gccgtcggtg
      541 gccttctccc cgcagaagac gaggtcgaat gggccgaggc tccgcagggc ggcgctcagg
      601 gccctggccg tggcccaggt gtccgagccg ccgaactcct tgccggtgac gaggacagcc
      661 tcgtcgcagc ccatggccat ggcctccctc agggcggtct cggcctgggg cgggcccatg
      721 gagagggcca ggatcgtggt ggccgggtct gcgtccttga tccggagcgc cgcctcgagg
      781 gcatagaggt cgagggggtt cacgatcgag ctgtcctcgg acctgatgac tgtccccgtc
      841 tcggggtcga gggtcaggga gtcggtctcg ggcacctgct tcacgaggac ggctatcttc
      901 atggcgtttc gggaacctcc gcctggcccc attccccggg accgcgtcat ggcagtgtgc
      961 cccaggaaag ggaaaacggc aagcggaatt gaaagccaat ttctcgaatt ggcggttttt
     1021 ccccgccaaa gtggaacgat ctttcagcgc ctagagcttg tccttgacga aacggccgcc
     1081 caccagggtg gcgaggacgc ggaaatcccg gtcgaagacc aggatgtcgg cctcgtagcc
     1141 ggggacaagg aggcctcgtt tgcccaggcc gaggatccgc gaggggttcg aggcggccat
     1201 ctccacggcg ttctcgaggg gcacgccgaa gtccaccagg ttgcgcaggc cctcgatcat
     1261 ggtgagcgag gagcccgcga tgatgtcgtc cttctttcgg tagaagagcc ggtcgcggag
     1321 ctcgacctcc tcgcggttgg cgaatagggg gcagttgtgc tgctcggtgg gcttgagcga
     1381 atcggtgacg aggacgacct tgctcaccgg cttgtcgcgc atgaggaggc ggacgaggtc
     1441 ggggtggacg tggtgtccgt cggcgatgat ctcgcaggac agctcggagt ggatcatgat
     1501 ggcgcccacc gcgccggggt cccggtggtg gagccggctc atcgcgttga agaaatgggt
     1561 cgagtggagg atgcccgcct gcatgccctc gagcatgttc tcgtaggtgg cgtcggtgtg
     1621 gccggcctgg aggacgatgc cgcgcttgat gcagtagagg gcgagctcgc gcatgccctt
     1681 gagctcgggg gccaccgtca tgttggtgat gtggccccga cccgcctggt agagccgctc
     1741 catcagctcg aggtcgacag ccctcacgaa ctcgggcctc tgcaccccga gcctggcctg
     1801 ggagacgaag gggccctcga ggtggacgcc catgaccttg gcccccgtct ccctccccat
     1861 cgcccccacg caggcctcga tcgaggcgac catgtcctcc tcggtcatgg ggtagatagt
     1921 cgggcagaag gccgtgaccc cgaagccgcc gaggtgctcg gacatcgcca tgatggcgtc
     1981 caccgacatg tcctcggtgc cgaagccggc gaagccgtgg atgtgggtgt cgatgaggcc
     2041 gggggcgatg taggcccccc cgacgtcgag gatgagggca tcgctct
//
LOCUS       DASSQV010000295         5371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_317408, whole genome shotgun sequence.
ACCESSION   DASSQV010000295 DASSQV010000000
VERSION     DASSQV010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317408"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFL04_05115"
     CDS             <1..214
                     /locus_tag="VFL04_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839117.1"
                     /translation="TFGVDIALVPLGQTYTMGSVEEAAGAVIDSGAKLAIPIHYGMYE
                     GSAADAAKFASLLEGKARVVLLDLKK"
     gene            308..1378
                     /gene="msrB"
                     /locus_tag="VFL04_05120"
     CDS             308..1378
                     /gene="msrB"
                     /locus_tag="VFL04_05120"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7839118.1"
                     /translation="MKRLAFILGVSMLLGAGSSFAAGPANQAANPETAVFGGGFRSLQ
                     GVFDRTYGVLSTEAVYAGGKARKPTAATPAENGHALAVLVSYDPSRVGYPELLDRYLR
                     SIDPTDGEGQFADRGPLYRPVVFYTSQAQRSAAAASLDALSRNGPFAGKHLAVAVLPA
                     PSLVPAEQAEQDFALHNPEAYAAAEAKSGRLAFLERAWGRSSDAGLPPAVSAYKKPPL
                     DALRKSLNPMQFQVTQEEGTEPPFANEFWNNERAGIYVDIVSGEPLFTSTDKFDSGTG
                     WPSFDRPLVPSNIVLKMDTSFGMTRIEVKSRYAGSHLGHLFDDGPAPTGLRYCMDSAS
                     LRFIPKEDMQRLGYGQFLKYVK"
     gene            1648..5208
                     /gene="nifJ"
                     /locus_tag="VFL04_05125"
     CDS             1648..5208
                     /gene="nifJ"
                     /locus_tag="VFL04_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HET7839119.1"
                     /translation="MSDTKNMVMIDGNTAAAHVAHAVSEVIAIYPITPSSPMGELSDE
                     YSAMGRKNIWGTVPQVVELQSEAGAAGAVHGALTTGSLTTTFTASQGLLLMIPNMYKI
                     AGECTSAVFHIAARAVSAAALNIFGDHQDVMAARQTGWAMLASNNVQEVMDMALISHA
                     ATLESRIPFVHFFDGFRTSHEVQKVEEISYAVMQQMISDELVRAHRARGLTPERPAIR
                     GTSQNPDVYFTAREANNKYYDKVPAIVEAQMAKLAKLTGRAYKLFEYSGAPDAERLII
                     VIGSAADTTEETVATLVKKGEKVGVLKVRLYRPFSVKHFIDAIPATVKSIAVLDRTKE
                     PGSIGEPLYEDVRTAIGEAMGAGKGPFKAWPTVIGGRYGLGSCEFVPAMAKAIFDELK
                     KPAPKNHFTMGIFDDVTNSSIAWDDGFQLDAEGVVECLFYGLGSDGTVGANKNTIKII
                     GDDTPNAAQGYFFYDSKKAGTYTISHLRFGPKAIKKPYLVGKADFIACHKFSFLEKLD
                     MLQNAKPGAVFLLNSPFGADKVWDNIPVEVQKQIVDKKLKFYVIDGLEIAERAGMGNR
                     INTIMQTAFFKISGVLPEGEAVKLIKKYTEKTYARKGADVVARNIAAIDLALTELHEV
                     KYGAANGKQHMLSPVPANSPKFVKEVIGEIIAARGEKLKVSQLPDDGTFPTGTTKYEK
                     RNIAEKIPVWNEEICIQCGQCTVVCPHAVVRLKAYDPKHAAGAPATFKSVDAKGKELA
                     GLKATLQIAPEDCTGCGACVNICPVNDKVNAGRKAINLEAQPPLRESEKLNWDFFLKI
                     PNTDNKVLNLSTVKGIAMRQPLFEFSGACAGCGETPYIKMMTQLFGDRAVIANATGCS
                     SIYGGNLPTTPYTTRGDGRGPAWCNSLFEDAAEFGFGMRLTVEKQAEYARELLVAKKG
                     AGVDAALADKLLGNAQASDDDIDQQRAWVAELRSGLAKSKETWAAELSSMAEALIKKS
                     VWIIGGDGWAYDIGFGGLDHVIASGRNVNILVLDTEVYSNTGGQMSKATPYGAVAKFA
                     AAGKNIGKKDLGSIAMSYGYVYVAQISLGANMAQAIKAFREAESYDGPSIIIAYSTCI
                     NQGIDMMRGQNQAKAVVESGVWPTYRYDPRLKLEGKNPFQLDSKEPAMDKIEPYMYSE
                     VRFKTLRDADPARAADLLGKLKAQVERRYKEFRYLSERNF"
BASE COUNT         1004 a         2027 c         1557 g          783 t
ORIGIN      
        1 gaccttcggg gtggacatcg ccctcgtgcc cctcggccag acctacacca tgggctcggt
       61 cgaggaggca gccggggccg tcatcgactc gggggccaag ctcgccatcc cgatccacta
      121 cggcatgtac gagggctcgg cggccgacgc cgccaagttc gcgagcctcc tcgaaggcaa
      181 ggccagggtg gtcctcctcg acctgaagaa gtgaggctcc ccgccccggg cggatacatt
      241 ctgcttatct tatatatcga tccatttggg atcgatgccg cccggcttgg gcggcggagg
      301 taggcagatg aagaggctcg cgttcatcct cggcgtttcg atgctcctcg gagcgggttc
      361 ctccttcgcc gctggtcccg ccaaccaggc cgcgaacccg gagacggccg tgttcggcgg
      421 gggcttccgc agcctccagg gcgtcttcga ccggacctac ggcgtcctct cgaccgaggc
      481 ggtctacgcc gggggcaagg cccggaagcc gacggcggcc acccccgccg agaacggcca
      541 cgccctcgcc gtcctcgtga gctacgaccc ctcaagggtc ggctaccccg agctcctcga
      601 ccgctacctc cgctcgatcg accccaccga cggcgagggc cagttcgccg accggggccc
      661 cctctaccgc cccgtcgtct tctacacgag ccaggcccag cgcagcgccg ctgcggcctc
      721 gctggacgcc ctgtccagga acgggccctt cgccggcaag cacctggccg tcgccgtcct
      781 gccagccccc tccctggtcc ccgccgagca ggccgagcag gacttcgccc tccacaaccc
      841 cgaggcctac gccgccgccg aggcgaaatc cggccgcctc gccttcctcg agcgggcatg
      901 gggcaggtcg agcgacgccg gcctcccccc ggccgtgagc gcctacaaga agccccccct
      961 cgatgcgctg cgcaagagcc tgaaccccat gcagttccag gtgacccagg aggaggggac
     1021 cgagccgccc ttcgccaacg agttctggaa caacgagcgg gcgggcatct acgtcgacat
     1081 cgtctcgggc gagcccctgt tcacctcgac cgacaagttc gactcgggca cgggctggcc
     1141 gagcttcgac cgccccctcg tgccctcgaa catcgtcctc aagatggaca cgagcttcgg
     1201 catgacgcgg atcgaggtga agagccgcta cgccggctcc cacctgggcc acctcttcga
     1261 cgacggccca gccccgaccg ggctgcgcta ctgcatggac tcggcttcgc tccgcttcat
     1321 cccgaaggag gacatgcagc ggctcggcta cggccagttc ctgaagtacg tgaagtgagg
     1381 ccctgccgtt tcgggcggaa tttcgccagc cgaaacgcac atttcagcaa ttaacacaat
     1441 tctgagcgaa tagctccgcc tgcgataaaa ccgccctgta tacaatggaa cgcaggcttt
     1501 tctaggcgat tgccctcccc catattgaat aaaaatgcga atggccgtac tatcctctcg
     1561 agctcggact gagccaactg attccgcgtc cggaatcgtc cgccgggtca gtgatcccca
     1621 accccctctt ctttcaagga atcctctatg tccgatacga agaacatggt catgatcgac
     1681 gggaacacgg ccgccgccca cgtggcccac gccgtgagcg aggtcatcgc gatctacccg
     1741 atcaccccct cctcccccat gggcgagctc tccgacgagt actccgccat gggccggaag
     1801 aacatctggg gcacggtccc ccaggtggtc gagctccagt ccgaggccgg cgccgcgggc
     1861 gcggtccacg gggccctcac cacgggctcc ctcaccacga ccttcaccgc ctcccagggc
     1921 ctcctcctca tgatcccgaa catgtacaag atcgccggcg agtgcacctc ggcggtcttc
     1981 cacatcgccg cccgcgccgt ctcggccgcg gccctcaaca tcttcggcga ccaccaggac
     2041 gtcatggccg cccgccagac cggctgggcc atgctcgcct cgaacaacgt ccaggaagtc
     2101 atggacatgg ccctcatctc ccacgcggcc accctcgagt cccggatccc cttcgtgcac
     2161 ttcttcgacg gcttccgcac ctcccacgag gtccagaagg tcgaggagat ctcctacgcc
     2221 gtcatgcagc agatgatctc cgacgagctc gtgcgcgccc accgcgcccg cggcctcacg
     2281 ccggagcgcc ccgccatccg cggcacgagc cagaaccccg acgtctactt caccgcccgc
     2341 gaggcgaaca acaagtacta cgacaaggtg cccgcgatcg tcgaggccca gatggccaag
     2401 ctcgccaagc tcaccggccg ggcctacaag ctcttcgagt actcgggcgc ccccgacgcc
     2461 gagcgcctca tcatcgtgat cggctcggcc gccgacacca ccgaggagac cgtcgccacc
     2521 ctcgtcaaga agggcgagaa ggtcggcgtg ctcaaggtga ggctctaccg ccccttcagc
     2581 gtcaagcact tcatcgacgc gatccccgcg accgtgaagt ccatcgccgt cctcgaccgc
     2641 accaaggagc ccggctccat cggcgagccc ctgtacgagg acgtccgcac cgccatcggc
     2701 gaggccatgg gcgccggcaa gggccccttc aaggcctggc ccaccgtcat cggcggccgc
     2761 tacggcctcg gctcctgcga gttcgtcccc gccatggcca aggccatctt cgacgagctc
     2821 aagaagcccg cccccaagaa ccacttcacg atgggcatct tcgacgacgt caccaactcc
     2881 tcgatcgcct gggacgacgg cttccagctc gacgccgagg gcgtggtcga gtgcctcttc
     2941 tacggcctcg gctccgacgg caccgtcggc gcgaacaaga acaccatcaa gatcatcggc
     3001 gacgacacgc ccaacgccgc ccagggctac ttcttctacg actccaagaa ggccggcacc
     3061 tacacgatca gccacctccg cttcgggccc aaggccatca agaagcccta cctcgtgggc
     3121 aaggccgact tcatcgcctg ccacaagttc agcttcctcg agaagctcga catgctgcag
     3181 aacgccaagc cgggcgccgt cttcctcctc aactccccct tcggcgccga caaggtctgg
     3241 gacaacatcc ccgtcgaggt gcagaagcag atcgtcgaca agaagctcaa gttctacgtg
     3301 atcgacggcc tcgagatcgc cgagcgcgcg ggcatgggca accgcatcaa cacgatcatg
     3361 cagacggcct tcttcaagat ctccggcgtc ctgcctgaag gcgaggccgt caagctcatc
     3421 aagaagtaca ccgagaagac ctacgccagg aagggcgccg acgtcgtggc ccgcaacatc
     3481 gcggccatcg acctcgccct caccgagctc cacgaggtca agtacggcgc tgccaacggc
     3541 aagcagcaca tgctgagccc cgtgcctgcc aactcgccca agttcgtcaa ggaagtcatc
     3601 ggcgagatca tcgcggcccg cggcgagaag ctcaaggtga gccagctccc cgacgacggc
     3661 accttcccca ccggcaccac caagtacgag aagcgcaaca tcgccgagaa gatccccgtc
     3721 tggaacgagg agatctgcat ccagtgcggc cagtgcaccg tggtctgccc ccacgccgtc
     3781 gtccgcctca aggcctacga tcccaagcac gccgccgggg cccccgcgac cttcaagtcg
     3841 gtggacgcga agggcaagga gctcgccggc ctcaaggcga ccctccagat cgcccccgag
     3901 gactgcaccg gctgcggcgc ctgcgtgaac atctgccccg tgaacgacaa ggtcaacgcc
     3961 gggcgcaagg ccatcaacct cgaggcccag cccccgctcc gcgagagcga gaagctcaac
     4021 tgggacttct tcctcaagat ccccaacacc gacaacaagg tcctgaacct ctcgacggtg
     4081 aagggcatcg cgatgcgcca gcccctcttc gagttctccg gcgcctgcgc gggctgcggc
     4141 gagaccccct acatcaagat gatgacccag ctcttcggcg accgcgccgt gatcgcgaac
     4201 gccacgggct gctcctcgat ctacggcggc aacctcccca cgacccccta caccacccgc
     4261 ggcgacggac gcggccctgc ctggtgcaac tccctcttcg aggacgccgc cgagttcggc
     4321 ttcggcatgc ggctcaccgt ggagaagcag gccgagtacg cccgcgagct cctggtcgcc
     4381 aagaagggcg ccggggtgga cgcggccctc gccgacaagc tcctcggcaa cgcccaggcc
     4441 agcgacgatg acatcgacca gcagcgcgcc tgggtggccg agctccgctc cggcctcgcc
     4501 aagagcaagg agacctgggc cgccgagctc tcgagcatgg ccgaggccct catcaagaag
     4561 tcggtctgga tcatcggcgg agacggctgg gcctacgaca tcggcttcgg cggcctcgac
     4621 cacgtgatcg cctcgggccg caacgtcaac atcctcgtcc tcgacaccga ggtctactcg
     4681 aacaccggcg gccagatgtc caaggccacg ccctacggcg ccgtggcgaa gttcgccgcc
     4741 gccggcaaga acatcggcaa gaaggacctg ggctcgatcg cgatgagcta cggctacgtc
     4801 tacgtggccc agatcagctt gggcgccaac atggcccagg cgatcaaggc cttccgcgag
     4861 gccgagagct acgacggccc ctcgatcatc atcgcctact cgacctgcat caaccagggc
     4921 atcgacatga tgcgcgggca gaaccaggcc aaggccgtgg tggagtcggg cgtctggccg
     4981 acctaccgct acgacccccg gctcaagctc gagggcaaga accccttcca gctcgactcc
     5041 aaggagccgg cgatggacaa gatcgagccc tacatgtaca gcgaggtccg cttcaagacc
     5101 ctccgcgacg ccgaccccgc ccgcgcggcc gacctcctgg gcaagctcaa ggcccaggtc
     5161 gagcgccggt acaaggagtt caggtacctc agcgagcgca acttctaata gctagaacat
     5221 tctgacgaaa aggccgtccc gcaaggggcg gctttttcat tgtttggtgg gggagcacgg
     5281 cccgcacgag caccgcccgc gccccagagt gcgatctgat ggcgcctcca cggagtcccg
     5341 cacggggccc ccgccttcga caccccgtcc c
//
LOCUS       DASSQV010000296         3249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1933119, whole genome shotgun sequence.
ACCESSION   DASSQV010000296 DASSQV010000000
VERSION     DASSQV010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933119"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFL04_05130"
     CDS             complement(<1..535)
                     /locus_tag="VFL04_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET7839120.1"
                     /translation="MSIQLPIDVSYFVVAVLFIMGLKAMSSPVTARKGILWAGVGMVI
                     ATLITFATPGMHNFVLMIAALVVGAVIAAWLGRTVKMTDMPQMVAIYNGMGGGAAAAI
                     AALEFTRAAAQGESLGLASTILALLGSLIGSVAFSGSCVAFAKLQGIMKKAHRLPAQN
                     VVNVVLGLAAIGLGAFIV"
     gene            complement(532..831)
                     /locus_tag="VFL04_05135"
     CDS             complement(532..831)
                     /locus_tag="VFL04_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET7839121.1"
                     /translation="MEGFIYLYVFMLAAFTGYEVISRVPVILHTPLMSGSNFVHGVVL
                     VGAMVALGAADPSDPLQLAIGVFAVVLGAANAAGGYVVTERMLAMFKTGKKKESK"
     gene            complement(831..1961)
                     /locus_tag="VFL04_05140"
     CDS             complement(831..1961)
                     /locus_tag="VFL04_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET7839122.1"
                     /translation="MSLLIGIARETAAGENRVAVVPEIAKKYRSLGAELFIESEAGAA
                     SSCRDGDFGDASIAKSAAEVFSRAGLILKVLPPSLAEIAQMKAGSVLLGYLQPYQDAA
                     RIKALNERRITSFAVELIPRISRAQSMDALSSQAAVSGYLCSLIAANNCPKFFPMLTY
                     AAGTIRPARVLVIGAGVAGLQAIATAKRLGAIVEAYDVRPETKEQIESLGARFVDTGV
                     SAAGSGGYARELTEEEKALQAEKLGKAVAMADVLITTAAIPGKRSPLIISAAMVAGMK
                     HGAVVVDMAAEGGGNVAGTVADQTVEVGGVRIMGPTNLPSRMPVHASEMYAKNLYNFI
                     SPFIKQGELAIDWNDEIISGSCLTRDGELVHVGVKMALKGCV"
     gene            complement(2208..>3249)
                     /locus_tag="VFL04_05145"
     CDS             complement(2208..>3249)
                     /locus_tag="VFL04_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7839123.1"
                     /translation="RLTGLIGRVSELAGAVQKGSVALADSSEHARTAVGGLETSVSSV
                     RQAVERQSETVGGAEGEIARMLSSIEQVAARVADQALFVDQSSAAVSEMAANIASVSR
                     IAVKADELAQRLREASDEGGQALRDSLAAIAEISAASAAVREIISVISKIAAQTNLLA
                     MNAAIEAAHAGEAGSGFAVVANEVRGLAENAARSAKEIESHIRGMAQKIDRGSGLADR
                     AGKSFDRIRDGVAETGELVRTIAASMTEQQEGAEEILRSVESLTEATRTIKTLTAEQR
                     AESKAMEGSMLRIVSASNEIFEAVQEETGATQALGRVVAVVGEEADHNRELVRGLEAA
                     VSYFKTGGGAGS"
BASE COUNT          491 a         1100 c         1139 g          519 t
ORIGIN      
        1 cgacgatgaa ggccccgagg ccgatggccg cgaggccgag caccacgttc acgacgttct
       61 gggcgggcag gcggtgggcc ttcttcatga tgccctggag cttggcgaag gcgacgcagg
      121 agcccgagaa ggccacggag ccgatgaggg agccgaggag ggcgaggatg gtcgaggcca
      181 gccccaggct ctcgccctgg gccgctgccc gggtgaactc gagggccgcg atcgcggcgg
      241 cggcgccgcc gcccatgccg ttgtagatgg cgaccatctg gggcatgtcg gtcatcttga
      301 ccgtccggcc gagccaggcg gcgattacgg cgcccacgac cagggcggcg atcatgagga
      361 cgaagttgtg catcccgggc gtcgcgaagg tgatgagggt ggcgatgacc atgccgaccc
      421 ccgcccagag gatgcccttg cgggcggtca ccggactgga catggccttg aggcccatga
      481 tgaagaggac ggcgacgacg aagtaggaga cgtcgatcgg cagctgtatg ctcatttgga
      541 ttccttcttc ttgccggtct tgaacatcgc gagcatccgc tcggtgacga cgtagccgcc
      601 ggcggcgttg gccgccccga gcacgacggc gaagacgccg atggcgagct ggaggggatc
      661 gctcgggtcg gccgcgccga gggcgaccat cgcgcccacg aggacgacgc cgtgcacgaa
      721 gttggagccc gacatcaggg gggtgtggag gatcaccggc acccggctga tgacctcgta
      781 gccggtgaag gccgcgagca tgaagacgta gaggtagatg aagccttcca tcacacgcat
      841 cctttcagcg ccatcttgac gccgacgtgg acgagctcgc cgtcgcgggt gaggcaggag
      901 cccgagatga tctcgtcgtt ccagtctatg gcgagctccc cctgcttgat gaagggggag
      961 atgaagttgt agaggttctt cgcgtacatc tcggaggcgt gcaccggcat cctcgagggc
     1021 aggttggtgg gccccatgat cctgaccccg ccgacctcga ccgtctggtc agcgacggtg
     1081 cccgcgacgt tgccgccgcc ctcggccgcc atgtccacga ccacggcccc gtgcttcatg
     1141 ccggccacca tggccgcgct gatgatgagg ggggagcgct tgccggggat ggccgcggtg
     1201 gtgatgagga cgtcggccat cgcgaccgcc ttgccgagct tctcggcctg gagggccttc
     1261 tcctcctcgg tgagctcgcg ggcgtagccg cccgagcccg cggccgagac cccggtgtcc
     1321 acgaagcgcg cgccgaggga ctcgatctgc tccttggtct cgggccggac gtcataggcc
     1381 tcgacgatgg cgccgagccg cttggcggtc gcgatggcct ggaggccggc gacgcccgcg
     1441 ccgatgacga ggacccgggc cggcctgatc gtgccggcgg cgtaggtgag catggggaag
     1501 aacttggggc agttgttcgc cgcgatgagg gagcagaggt agccggagac ggccgcctgc
     1561 gaggacaggg cgtccatgct ctgagccctg gagatccgcg ggatcagctc gacggcgaag
     1621 ctcgtgatcc tgcgctcgtt gagcgccttg atcctggcag cgtcctggta gggctggagg
     1681 tagcccagga ggaccgagcc ggctttcatc tgggcgatct ccgccaggct gggcgggagg
     1741 accttgagga tgaggccggc acgggaaaac acctcggcgg cgctcttcgc gatgctcgca
     1801 tcgccgaagt ccccgtcgcg gcaggagctc gcggcccccg cctctgattc gatgaagagt
     1861 tccgcgccga ggctgcggta cttcttcgcg atctcgggaa ccacagcgac gcggttctcc
     1921 cccgcggcgg tttcccgcgc aatgccgatg agcagagaca tgtcacgctc ctcgatcgta
     1981 gtgttgccgc cgcggcagcc ccgcgaacgg ggccccggtc ttgaggcggc gattcgataa
     2041 actaatactg attttacctg ttagtactaa aatagcgacg gcgcgcccat tctggcgcgc
     2101 cgcactcgta ttcccacctc cggaaagccc gctcccgcgg accgaacgaa ggctagcccg
     2161 aactctccgg gctgtcaagc aaaaataatc cgcttctgcc ggcagcatca cgagccggcc
     2221 cctccgccgg tcttgaagta ggagacagcc gcctcgagcc cgcgcacgag ctcgcggttg
     2281 tggtcggcct cctcgcccac gacggccacg acccggccca gggcctgggt cgcccccgtc
     2341 tcctcctgga cggcctcgaa gatctcgttc gaggccgaga cgatcctcag catcgagccc
     2401 tccatggcct tggactcggc gcgctgctcg gcggtgaggg tcttgatggt cctggtggcc
     2461 tcggtgaggg actcgacgct cctgaggatc tcctcggcgc cctcctgctg ctcggtcatg
     2521 gaggcggcta tcgtgcgcac gagctccccg gtctcggcca ccccgtccct gatccggtcg
     2581 aaggacttgc cggcgcggtc ggcgaggccc gagccccggt cgatcttctg ggccatgccc
     2641 ctgatgtggc tctcgatctc cttggcgctc cgggccgcgt tctccgcgag gccgcggacc
     2701 tcgttggcga ccacggcgaa gcccgagccc gcctcgccgg cgtgggcggc ctcgatggcg
     2761 gcgttcatcg cgaggaggtt ggtctgggcg gcgatcttgg agatgaccga gatgatctcg
     2821 cggaccgccg ccgaggcggc cgagatctcg gcgatggccg cgagcgagtc gcgcagggcc
     2881 tggcccccct cgtcgctcgc ctcgcggagc ctctgggcca gctcgtcggc cttgaccgcg
     2941 atgcgcgaca cggaggcgat gttcgccgcc atctcgctca ccgccgccga gctctggtcc
     3001 acgaaaaggg cctggtcggc cacgcgggcg gcgacctgct cgatcgatga gagcatcctg
     3061 gcgatctccc cctcggcccc gcccaccgtc tccgactgcc tctcgaccgc ctgcctcacc
     3121 gaggacaccg aggtctcgag cccgcccacg gccgtgcggg cgtgctccga ggagtcggcc
     3181 agggccaccg agcccttctg gaccgcgccg gcgagctcag agaccctgcc gatcaggccc
     3241 gtgagccgc
//
LOCUS       DASSQV010000297         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1810928, whole genome shotgun sequence.
ACCESSION   DASSQV010000297 DASSQV010000000
VERSION     DASSQV010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810928"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1111
                     /gene="add"
                     /locus_tag="VFL04_05150"
     CDS             62..1111
                     /gene="add"
                     /locus_tag="VFL04_05150"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET7839124.1"
                     /translation="MAARSISDFIASLPKTEIHIHAEATVSFDSYFSLNQKYNADSSL
                     KRPDDFRKLLEMDSLGVMIKNFFYLQSLFRSPEDFRLVVSDVQAYARRNNIRYMELFV
                     APSMVLKGGNIDFEGIMDPLVEGFERAAATPDGVDIRILVDVSRSFGKDNASRNFHHL
                     LSYLSRRRTDRILGIGLGGQEMGNPCIAYKDVFREAREAGLHAVAHAGEEVGPESIRD
                     AVLELGAERIGHGTSAIQDPSLMDLLKDRGIPLEICPTSNVVTGKYVRSYADHPMKAF
                     LARGLLVTLNTDDPVLFDVELNGEFGNAARHLGWTASQIVTVLRNGIRATFMDESGKA
                     AALARLDAQLPKPKA"
     gene            1206..>2275
                     /locus_tag="VFL04_05155"
     CDS             1206..>2275
                     /locus_tag="VFL04_05155"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HET7839125.1"
                     /translation="MSIFLLDYGPILIGCSVTLLVLATLVAFSLPGRSNPYSSIILVG
                     TALWSLFHGLEIGAADQGLKELWSDLQYLGLGLLPFSWVAFTARVAGDRKPRLVARLW
                     PYLLFTAFEVLAAATNRWTGALFGYSGIQGMGQQADKHRGWAFWLCTALLLFSMGRGS
                     LTLFRASRNEGLDGSGRRRARLFLLVVILPLLLGILDVLNIRPIPGYNFAPLSLSVSI
                     IILGYGLFFLRLLSPIPLAHRALIDGMKDPVIVVDERRWAVYRNAAAAAIARSGEWSR
                     HGAPLDRFLPEISTPEDIADGSVLRIGEREYEVRSSPVTKKDGAVTVRLFVLRDVTAS
                     RAERERLEEIVRGRAVQLRDANL"
BASE COUNT          377 a          845 c          656 g          397 t
ORIGIN      
        1 cgcactatca tctatgccga taatgagatc ggaagagtac acacagccgg gggcagccag
       61 gatggcagcg cgttccatct cggacttcat agcgtcattg ccgaagaccg agatccacat
      121 acacgccgag gcgaccgtca gcttcgacag ctacttctcg ctcaaccaga agtacaacgc
      181 cgattcctcg ctcaagcgcc ccgacgattt ccgcaagctc ctcgagatgg actcgctcgg
      241 ggtcatgatc aagaacttct tctatctcca gtccctcttc cgctcgcccg aggacttcag
      301 gctcgtcgtg agcgacgtcc aggcctacgc ccggcgcaac aacatccgct acatggagct
      361 cttcgtcgcc ccctcgatgg tgctgaaggg ggggaacatc gacttcgagg ggatcatgga
      421 ccccctggtc gagggcttcg agcgcgccgc cgcgacgccg gacggggtcg acatacggat
      481 cctcgtcgac gtctcccggt ccttcggcaa ggacaacgcc tcccgcaact tccaccacct
      541 cctctcctac ctctcgcggc gccggacgga caggatcctg ggcatcggcc tgggcggcca
      601 ggagatgggc aatccctgca tagcctacaa ggacgtgttc cgcgaggcca gggaggccgg
      661 cctccacgcc gtggcccacg cgggcgagga ggtggggccc gagtccatca gggatgccgt
      721 gctcgagctg ggggccgaga ggatcggcca cggcacctcg gcgatccagg acccctcgct
      781 catggacctg ctcaaggacc gcggcatccc gctcgagatc tgccccacga gcaacgtggt
      841 caccggcaag tacgtcagaa gctacgccga ccatcccatg aaggccttcc tcgcccgggg
      901 cctcctcgtg accctcaaca ccgacgaccc cgtgcttttc gacgtggagc tcaacggcga
      961 gttcggaaac gcggcccgcc acctcggctg gacggcctcg cagatcgtca cggtcctccg
     1021 caacggcatc cgcgccacct tcatggacga gtccggcaag gccgcggccc tcgccaggct
     1081 cgacgcccag ctgcccaagc ccaaggcctg acacccaagt ttctccaagc ccttcataca
     1141 cccactagag ctttttcggg cgtttggatg taatatgtaa ttagcccatt tcgcaggagc
     1201 cgggcgtgtc catctttttg ctggattacg gtccaatcct catcgggtgc agcgtcaccc
     1261 tcctcgtcct cgcgacgctc gtcgcctttt ccctgcccgg ccgatccaac ccctacagca
     1321 gcatcatcct cgtcgggacc gccctctgga gcctcttcca tggcctcgag atcggcgcgg
     1381 ccgaccaggg cctgaaggag ctctggtccg acctgcagta cctcggactg ggcctccttc
     1441 ccttctcctg ggtggccttc accgcccggg tcgcgggcga ccgcaaaccc cgtctggtcg
     1501 cgaggctctg gccctacctc ctcttcaccg ccttcgaggt cctggcggcg gccaccaacc
     1561 gctggaccgg ggccctcttt ggctattcgg gcatccaagg tatgggacaa caggcggaca
     1621 agcaccgcgg ctgggccttc tggctctgca ctgccctcct cctcttctcg atggggcgcg
     1681 gaagcctcac cctcttccgg gcgagccgga acgagggcct cgacggctcg gggcgcaggc
     1741 gggcccgcct cttcctcctc gtggtcatcc ttcccctgct cctcggcatc ctcgatgtcc
     1801 tgaacatcag gcccatcccg ggctacaatt tcgcccccct ctccctctcg gtctcgatca
     1861 tcatcctggg ctacggcctg ttcttcctcc gcctcctctc gcccatcccg ctggcccacc
     1921 gcgccctcat agacggcatg aaggaccccg tcatcgtggt cgacgagcgc cgctgggccg
     1981 tctaccggaa cgccgcggcg gccgccatag cccgctcggg cgaatggagc aggcacggcg
     2041 cccccctcga ccgcttcctc cccgagatct ccaccccaga ggacatagcc gacggcagcg
     2101 tcctgcgcat aggggagcgg gaatacgagg tccgctcctc ccccgtgacc aagaaggacg
     2161 gggcggtcac cgtgaggctc ttcgtcctca gggacgtcac ggcctccagg gcagagcggg
     2221 agaggctcga ggagatcgtc cgcggaaggg cggtccagct gcgcgacgcc aacct
//
LOCUS       DASSQV010000298         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2308067, whole genome shotgun sequence.
ACCESSION   DASSQV010000298 DASSQV010000000
VERSION     DASSQV010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2308067"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFL04_05160"
     CDS             complement(<1..521)
                     /locus_tag="VFL04_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839126.1"
                     /translation="MRIETYFARPGLDGRSRGPQQALQRALARPVSLEIADVILTDIA
                     IPPTLAREVFCDPVVQGIAEGPIPELGLFSGWDWLVEVAYRPGVTDTLALTAREALEI
                     AEPGLGEGGTVQTAKLYLVSAAGIDREALAKAFSSMWNPLIQSAIFVSRKEWAAGKRL
                     PELYPKVELSEPP"
     gene            complement(570..2405)
                     /locus_tag="VFL04_05165"
     CDS             complement(570..2405)
                     /locus_tag="VFL04_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839127.1"
                     /translation="MNSGAIKRAFGLTFLYIGIFVLIVIVQFSRGPGFSEKFDQLSVR
                     ATYPRSGRGDIGLAPDALKLNYAGFQIAVDAASPVELLGPDGTAKAVGLKSVDRIKGG
                     VRVRLEGGTEFRVTVDASNGESYALAANAPDGSTGIRLRYSLVGGARLRDMDGHPVLD
                     SGRTSFDLSLPSTALARDKGLLVLDPRDPSSSSIAFVRRLPAQGKPTPPKAPDTFIAQ
                     APKDPASFKAEIAAWRDKVWAGLSSTRFDPDRVTWSGPDGSARFSERALVAYYAEALA
                     RDAPSEVFARVRGARERWPDRLSYLSAPYVGGLLPKMQALEAADLVEVKRLSQLIQDK
                     SPALFEKENLIHFLLDRSPFASAQDALRYAAGIDPSKLSVRQAVGLLGCMVESRSYLK
                     DEENPFKAFGATADRIVASVRKTGSGFFLVTDDDGSTDMRISLLAGSSLLRYGANEGK
                     DMLVGVGQSLVEGVLGLGDTQGFAPAKAVVKAGDLEQKSGSLAPEDIYPLIAGNPYYA
                     KEVSFYRDIAPGAWSWTCSPSLTVDAKPNRYVFTAEFPQGRAHYMAFYGMKAFTNIQL
                     YDIDYNPDNDFESYDASGYLYRKPTSALYVKMKHKKESETIKLFF"
BASE COUNT          452 a          819 c          910 g          445 t
ORIGIN      
        1 atggggggct cgctcagctc caccttcgga tagagctccg gcaggcgctt ccccgccgcc
       61 cactccttcc tcgagacgaa gatggccgac tggatcaggg ggttccacat cgacgagaag
      121 gccttcgcga gcgcctctct atcgatcccc gccgcggaga caaggtagag cttcgccgtc
      181 tggacggtgc ccccctcgcc cagacctggc tccgcgatct caagggcctc gcgggccgtc
      241 agggcgagtg tgtcggtgac cccgggacga taggccacct cgaccagcca gtcccagccg
      301 gagaagagcc cgagctcggg aatcgggccc tcggcaatgc cctggaccac ggggtcgcag
      361 aagacctcgc gcgcgagcgt gggggggatg gcgatgtcgg tgaggatgac gtcggcgatc
      421 tcaaggctga ctggccgggc gagggcgcgc tggagggcct gctgcggccc ccggctcctg
      481 ccgtcgaggc cgggacgagc gaaataggtc tcgatccgca tggggcactc tacagaatgg
      541 ccccatcagc agtcaagctc gctcccggcc tagaagaaga gcttgatcgt ctcgctctcc
      601 tttttgtgct tcatcttcac gtagagggcg ctcgtcggct tgcggtagag gtagcccgag
      661 gcgtcgtagc tctcgaaatc gttgtcgggg ttgtagtcga tgtcgtagag ctggatgttg
      721 gtgaaggcct tcatgccgta gaaggccatg tagtgggccc tcccctgggg gaactccgcg
      781 gtgaagacat agcggtttgg cttggcgtct accgtaaggg agggggaaca ggtccatgac
      841 caggcccccg gcgcgatgtc gcggtagaag gagacttcct tggcgtaata gggattgccg
      901 gcaatgaggg gatagatgtc ctcgggcgcc agggagccgc tcttctgttc caggtctccc
      961 gccttcacca cagccttggc gggggcgaag ccctgggtgt ccccaaggcc gaggacgccc
     1021 tcgacgaggc tctgacccac gcccacgagc atgtccttgc cctcgttggc gccgtagcgg
     1081 aggaggctcg agccggccaa gagggaaatg cgcatgtccg tcgaaccgtc gtcatcggtg
     1141 accaggaaga agccgctccc ggtcttcctg accgaggcca cgatccggtc cgctgtggcg
     1201 ccaaaggcct tgaaggggtt ctcctcatcc ttgaggtagg atcgcgattc gaccatgcac
     1261 ccgagaagac cgacggcctg ccgcacgctg agcttcgagg gatcgatccc cgcggcatag
     1321 cggagggcgt cctgggccga ggcgaagggc gagcggtcga ggaggaagtg gatgaggttc
     1381 tccttctcga acagggcggg ggacttatcc tggatgagct gggagaggcg cttcacctcg
     1441 acgaggtcgg cggcttcgag ggcctgcatc ttcggcagga ggccgcccac ataaggggcg
     1501 ctgaggtagc tcagcctgtc gggccagcgc tcacgggcgc cccgcacccg ggcgaagacc
     1561 tcgctcgggg cgtcccgcgc gagcgcctcg gcgtaatacg cgacgagggc gcgctccgag
     1621 aagcgggccg agccgtccgg gccgctccag gtcacccgat cggggtcgaa gcgggtcgaa
     1681 ctgagcccgg cccagacctt gtcgcgccag gcagctatct cggctttgaa ggatgcgggg
     1741 tccttgggcg cttgggcgat gaaggtgtcg ggagcctttg ggggcgtcgg cttgccctgg
     1801 gcgggaaggc ggcgcacgaa ggcgatggag ctcgaggaag gatcccgagg atcgaggact
     1861 aggaggccct tgtccctggc gagggcggtg ctcggaaggg agaggtcaaa gcttgtcctg
     1921 cccgaatcga ggacgggatg gccgtccatg tccctcagcc tcgctccacc gacgagggaa
     1981 tatcggaggc gtatgccggt gctgccgtcg ggggcgttcg ccgctagggc gtagctctcc
     2041 ccattggatg cgtcgacggt cacccggaac tcggtgccgc cctccagcct cacccttact
     2101 ccgcccttga tcctgtccac cgactttagg ccaacggcct tggccgtgcc atcggggccg
     2161 aggagctcga cgggggaggc ggcgtcgacg gctatctgga agcccgcata gttgagcttc
     2221 agcgcatcgg gggcaagccc aatatcccca cgccccgatc tcggataggt cgccctcacc
     2281 gagagctggt cgaatttctc ggaaaaaccc ggcccccgcg aaaactggac gatgacgatg
     2341 aggacgaata ttccgatata gaggaacgtg agcccgaagg ctcgctttat ggctccgctg
     2401 ttcattgcgg ctaaagacta tagcccctaa cggtgccttg cgcaagcttg tgcaaggcga
     2461 tcgtatcggg gtacgatcgg gcacccgggc gtccaacgcg ccaagctcgc ccgcagggga
     2521 gatcgaacat gaccaagacg ccaagcttcc gcacacatcg caggcccaat gcgagccttg
     2581 ccatcctcct ctgcgcggct gcggccgcgt cggtcctcgg ggcttg
//
LOCUS       DASSQV010000299         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1313504, whole genome shotgun sequence.
ACCESSION   DASSQV010000299 DASSQV010000000
VERSION     DASSQV010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313504"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VFL04_05170"
     CDS             complement(<1..589)
                     /locus_tag="VFL04_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7839128.1"
                     /translation="MKVLCIAQQKGGVCKTTTALSLSAAWALEGRRVLMVDVDPQANL
                     TRNIIEYEKIKNDVSMLYSGKKRLGQTLVPTSSANLFLAPARTSLSRVENLERTLSTY
                     NTLKRELPKVEKDFDLAVIDTPPSLGIFTVSALIASTHVLVPVQPTFFCQEGLGDLNQ
                     TIVEARESNPGLASVRYFMTMFDKRTVISQEIAQLL"
     gene            complement(751..1257)
                     /locus_tag="VFL04_05175"
     CDS             complement(751..1257)
                     /locus_tag="VFL04_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET7839129.1"
                     /translation="MANEHILAAIKAGMKGEIDSTTVYETAAITATRENAPEVRDFFA
                     ERAEEEKKHYNWLLAYYKEIAAGGQPGADFALGSAPVTSPIISQDFLRRVGESRQLSA
                     ALSTAILLEATAVKHYLNCAEEAVQAPLRSFYETLSAWEDRHYHDLISIQEESERYFW
                     DANNWQPF"
     gene            1395..2459
                     /locus_tag="VFL04_05180"
     CDS             1395..2459
                     /locus_tag="VFL04_05180"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET7839130.1"
                     /translation="MHPEELADGLEYILARSPALPGRIASSILIANPKAGGFTRRSFA
                     RRRHAELQALVGRAASLPLRPDPIPFRLRLTDSQGHAAALAHEYFAETESQGPKDSYH
                     LLMMAGGDGTSLETLVALMQLPPERRSRYLVLRLPFGTGNDGSDGRELDSCLGRFLGP
                     CRVASSPAMSIRRAAAGKETLWTFNIASLGADAFIAHMTNRLKTSFPGDSYKIWVDIA
                     SVLYDSIWPSRPIRVRAFSADGKVAKDFVRDLLLVAVGVTGRRQYGSNKQIFPDDDNV
                     IVLSRMSLFRKLAVKGPIYAGRCRSLPEAELFSARRIVVEHDSALLFQADGEVWRFEA
                     EDFPAEFEILPDSYQVLAGA"
BASE COUNT          364 a          898 c          876 g          450 t
ORIGIN      
        1 gcaagagttg ggcgatctcc tggctgatga cggtgcgctt gtcgaacatg gtcatgaagt
       61 agcggaccga ggcgagtccg gggttgctct ccctggcctc gacgatggtc tggttgaggt
      121 ccccgaggcc ctcctggcag aagaaggtgg gctggacggg cacgaggacg tgggtcgagg
      181 cgatgagggc cgacactgtg aagatgccca gggagggcgg ggtgtcgatg acggcgaggt
      241 cgaagtcctt ctcgaccttg ggcagctcgc gcttcagggt gttgtaggtc gagagggtcc
      301 gctcgaggtt ctcgacccgg cttaaactgg tgcgggccgg ggccaggaag aggttcgcgg
      361 atgaggtcgg aacgagggtc tggccgagcc gcttcttgcc cgagtagagc atcgagacgt
      421 cgttcttgat cttctcgtac tcgatgatgt tccgggtgag gttggcctgg gggtcgacgt
      481 cgaccatgag gacccgtcgg ccctcgaggg cccaggccgc cgagagcgag agtgcggtcg
      541 tggtcttgca gactccgccc ttctgttggg cgatgcaaag gactttcatg cgcctacctc
      601 ccacgagtat cggcgcgccg aaagccggcc ttgatgcaag tctcggtcgg gttgaggttt
      661 acccatgggc cccgaggcgg caggcgcccc gggcggcggc ccgcgctccg gcggcccgcc
      721 cgccctcggc ctgcccccct caggccccgg ctagaagggc tgccagttgt tggcgtccca
      781 gaagtagcgc tcggactcct cctggatcga gatgaggtcg tggtagtgcc tgtcctccca
      841 ggcgctcagg gtctcgtaga aggagcgcag gggggcctgg acggcctcct cggcgcagtt
      901 gaggtagtgc ttgaccgcgg tggcctcgag gaggatggcg gtggacaggg cggccgagag
      961 ctggcggctc tcgcccacgc ggcgcaggaa gtcctggctg atgatggggc tcgtgacggg
     1021 ggccgagccg agggcgaagt cggcgccggg ctggccgccc gccgctatct ccttgtagta
     1081 ggcgaggagc cagttgtagt gcttcttctc ctcctcggcg cgctcggcga agaagtcgcg
     1141 cacctcgggg gcgttctccc gggtggcggt gatggcggcc gtctcataga cggtcgtgct
     1201 gtcgatctcg cccttcatgc cggcctttat cgccgcgagg atgtgctcgt tcgccatgcc
     1261 agggtcctcc ttggggcccc gcttcccggg gcacgcctac tactataggc cttgggcgcc
     1321 ccgatgcaac cggtccgtac attgcgtttc cgtaatggac attggcgggg aggcggttta
     1381 cactccccac cacaatgcat ccagaagaat tagcagacgg cctcgaatac atccttgccc
     1441 ggagccccgc cctcccgggc cggatcgcgt catccatcct gatcgcgaac ccgaaggcgg
     1501 gcggcttcac ccggcgcagc ttcgccaggc gccgccacgc cgagctccag gccctcgtcg
     1561 gccgcgccgc ctcgctcccc ctccggcccg accccatccc cttccggctc aggctcaccg
     1621 acagccaggg ccacgccgcc gccctcgccc acgagtactt cgccgagacg gaatcccagg
     1681 gccccaagga ctcctaccac ctcctcatga tggccggggg cgacgggacc tcgctcgaga
     1741 ccctcgtggc cctcatgcag ctgccccccg agcgccgctc gcgctacctc gtcctccgcc
     1801 tgcccttcgg gacgggcaac gacggctccg acggccggga gctcgactcc tgcctcggcc
     1861 gcttcctggg cccctgccgg gtggcgtcct cgccggccat gtccatccgc cgggccgcag
     1921 cggggaaaga gaccctgtgg accttcaaca tcgcgagcct cggcgccgac gccttcatcg
     1981 cccacatgac gaatcggctc aagacctcct tccccggcga ctcctacaag atctgggtcg
     2041 acatcgcctc ggtgctctac gactcgatct ggccctcccg ccccatcagg gtcagggcct
     2101 tctccgccga cggcaaggtc gcgaaggact tcgtccgcga cctcctcctc gtcgcggtgg
     2161 gggtcacggg caggcgccag tacggctcca acaagcagat cttccccgac gacgacaacg
     2221 tgatcgtcct gtcgcggatg tcgctcttcc gcaagctcgc ggtgaagggc cccatctacg
     2281 cgggcaggtg ccggagcctc cccgaggccg agctcttctc ggcccggcgg atcgtcgtcg
     2341 agcacgactc ggccctcctc ttccaggccg acggcgaggt ctggcgcttc gaggccgagg
     2401 acttcccggc cgagttcgag atcctccccg actcctacca ggtgctcgcg ggggcatgat
     2461 gggcgcggcg ggacggggca gggcaggggc cggcaggggc cggaggcggg accgcatcgg
     2521 tcccctgttc ctcttcgccc tcctcacctc ggccttcgtc ccctcggtcc tctccgaggt
     2581 cgacagct
//
LOCUS       DASSQV010000300         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_443795, whole genome shotgun sequence.
ACCESSION   DASSQV010000300 DASSQV010000000
VERSION     DASSQV010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443795"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /gene="ssnA"
                     /locus_tag="VFL04_05185"
     CDS             <1..1209
                     /gene="ssnA"
                     /locus_tag="VFL04_05185"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HET7839131.1"
                     /translation="ISGGYLSPGLVCSHTHLYSALARGMLVDIAPSKDFAQQLRNLWW
                     RLDRAIDLPILEASALAGCADAAASGVTSLVDHHAGPEAIDGSLSVIAKAYEEVGIRG
                     LLCYETTDRNGMEGAKAGVRENVRFAKEIDAARAAAKAKGSRPPLTDGAIGGHAGFTV
                     GEETLAALADAVKTTGRGIHIHAGEDKYDAVDSRHRFGADLATRLDRAGCLGPKSIIG
                     HGVWLTASEAELLNERDSFLAHNARSNMNNAVGYNGLLTSFRNVVLGTDGMSADMLEE
                     FRFACFRHRESLGPWWPGDFLACLDRGNRLMERYMGAAAGGPAAFGKVEAGAAADLVL
                     WDYEPPTPLVGENLAGHLAFGLGARSVRSVMVDGAFVIEDRKTAFDLARISENAREQA
                     KRLWKRMEER"
     gene            1212..>2309
                     /locus_tag="VFL04_05190"
     CDS             1212..>2309
                     /locus_tag="VFL04_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HET7839132.1"
                     /translation="MNLNAKAVTEKAKSYRDATAENLSKIIKVPAYSGTEKERIALLE
                     RLCREAGFAEVRVDGLGNLLARVGNGPKKLVFDAHIDTVETGDRSQWKLDPFSGLVKD
                     GLVHGRGASDQLGGAASMITAGRILSELGYNGQFSVWFAFTVMEEDCDGLCWKYLIEK
                     EGFRPDFFVSTEPTSCRLYRGHRGRMEIEARFKGVSCHGSAPERGKSAAYMAAKAALA
                     IEKLNTELKPDDDGFLGKGTITVSKIDVRGPSLCAVPDQGMIYMDRRLTWGETMELAV
                     EQVRKAITAAGVENVEVSVPNYDKPSWKGTVHAQELYFPTWKIPADHPLVRAGEEAHG
                     ALFGSKPVVDKWTFSTNAVAVCGRNRIPSIGF"
BASE COUNT          395 a          839 c          756 g          319 t
ORIGIN      
        1 atttccggcg gctacctcag ccccggcctc gtctgctccc acacgcatct gtattccgcg
       61 ctcgcccggg ggatgctcgt cgacatcgcg ccctcgaagg atttcgcgca gcagctgcgc
      121 aacctctggt ggcggctcga ccgcgcgatc gacctgccca tcctcgaggc gagcgccctc
      181 gccggctgcg ccgacgcggc ggcctcgggg gtcacgagcc tcgtcgacca ccacgcgggg
      241 cccgaggcca tcgacgggtc cttgagcgtc atcgccaagg cctacgagga ggtcggcatc
      301 aggggcctcc tctgctacga gacgaccgac cgcaacggca tggagggcgc gaaggccggg
      361 gtcagggaga acgtccgttt cgccaaggag atcgacgccg cgcgcgccgc ggccaaggcc
      421 aagggctcgc ggccccccct caccgacggg gccatcggcg gccacgccgg cttcacggta
      481 ggggaggaga ccctcgccgc cctcgccgac gcggtcaaga ccacgggccg cggcatccac
      541 atccacgccg gcgaggacaa gtacgacgcc gtcgactccc gccaccgctt cggcgccgac
      601 ctcgcgaccc ggctggacag ggccggctgt ctcggcccga agtcgatcat cggccacggc
      661 gtctggctca ccgcctccga ggccgagctc ctcaatgagc gcgactcatt cctggcgcac
      721 aacgcgcgct cgaacatgaa caacgccgtg ggctacaacg gcctcctcac gagcttcagg
      781 aacgtcgtcc tcggcaccga cggcatgagc gccgacatgc tcgaggagtt ccgcttcgcc
      841 tgcttcaggc accgcgagag cctgggtccc tggtggccgg gcgacttcct cgcctgcctc
      901 gaccggggca accgcctcat ggagcgctac atgggcgccg ccgccggcgg gccggcggcc
      961 ttcggaaagg tcgaggccgg ggctgccgcc gacctcgtgc tctgggacta cgagcccccc
     1021 acccccctgg tcggcgagaa cctcgccggc cacctcgcct tcggcctcgg cgcccggtcg
     1081 gtgcgctcgg tgatggtcga cggcgccttc gtgatcgagg acaggaagac ggccttcgac
     1141 ctcgcgcgga tctcagagaa cgcgcgggag caggcgaagc ggctctggaa aagaatggag
     1201 gagagataag catgaacctg aacgcgaaag cagtcaccga gaaggccaag tcctacaggg
     1261 acgccaccgc cgagaacctg tcgaagatca tcaaggtccc cgcctactcc ggcaccgaga
     1321 aggagcgcat cgccctcctc gagaggctct gcagggaggc cggcttcgcc gaggtgcggg
     1381 tcgacggcct gggcaacctc ctcgcccgcg tgggcaacgg gccaaagaag ctcgtcttcg
     1441 acgcccacat cgacaccgtc gagacgggcg acaggagcca gtggaagctc gaccccttct
     1501 cgggcctcgt gaaggacggc ctcgtccacg gccgcggcgc ctcggaccag ctcggcggcg
     1561 cagccagcat gatcacggcc gggcgcatcc tctccgagct cggctacaac gggcagttct
     1621 ccgtgtggtt cgccttcacc gtcatggagg aggactgcga cggcctgtgc tggaagtacc
     1681 tcatcgagaa ggagggcttc aggcccgact tcttcgtctc caccgagccc acgagctgca
     1741 ggctctaccg cggccaccgc ggccgcatgg agatcgaggc ccgcttcaag ggcgtgtcct
     1801 gccacggctc ggcccccgag cgcgggaaga gcgcggccta catggctgcc aaggccgccc
     1861 tcgcgatcga gaagctcaac accgagctca agcccgatga cgacggcttc ctcggcaagg
     1921 gcacgatcac cgtctccaag atcgacgtgc gcggacccag cctctgcgcc gtgcccgacc
     1981 agggcatgat ctacatggac aggcgcctta cctggggcga gaccatggag ctggcggtgg
     2041 agcaggtccg caaggccatc accgccgccg gggtcgagaa tgtcgaggtg agcgttccca
     2101 attatgacaa gccgagctgg aagggcaccg tccacgccca ggagctctac ttccccacct
     2161 ggaagatccc cgccgaccac cccctggtgc gggccggaga ggaggcccac ggggcccttt
     2221 tcggctccaa gcccgtggtg gacaagtgga ccttctccac gaacgccgtg gctgtctgcg
     2281 gccgcaacag gatcccctcg atcggcttt
//
LOCUS       DASSQV010000301         4770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_69920, whole genome shotgun sequence.
ACCESSION   DASSQV010000301 DASSQV010000000
VERSION     DASSQV010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69920"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="VFL04_05195"
     CDS             <1..1335
                     /locus_tag="VFL04_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7839133.1"
                     /translation="GLLLPAFHGGPAMGYLPQTLGSVVVASLLGAGPGILALAAAAGA
                     TALLPLAGELLETARLPAAAALLGVLAAGTVRDAAEARERRLMERIRDSVKRGERFSR
                     MSDTLIRLNEELEARVSGQRESVSTLYARIRKLDSLELKTVLGALLEGVSAFSQAEKA
                     AVYEYDAAAGTLVLQAWIGAKPEPSLELAGRVEGWVFRNDRPFSLRMVGSELGFDRLD
                     SRRSVLAYPLKSGAQAWGVLNIEEMPFYRYNPTTEKNLEIIVSLSAAYIGRAVDFRER
                     VLKRPRNEVTGLPGYGELVRLLGEELARRDGGHASVSVVLVEFLDFEALVFAHSGVKA
                     LGLVKATAVESAKAGRAIAFHYKEGSQLAFILPGKDRDGASLFCLEFAQLVGRMALEI
                     DGEPVRLEPVFGLAAALRDSGAEALLAEAERLLCLSRASFAERVGPGSGSGP"
     gene            1332..2075
                     /locus_tag="VFL04_05200"
     CDS             1332..2075
                     /locus_tag="VFL04_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839134.1"
                     /translation="MSGFDYIRHLADYYLPKTPHAALLAVQSDYLPLEAFVYKSSSVK
                     SLFEEPYDLAELDRILARPRLELGEAMLLGEIFWTMTHGDDKELALFAAESLGALENR
                     WARRIEELAALVALPGCGDGDCFAYARALYEYALIAGGHGPIRNYYLREAFYALSRHG
                     VARLEAEGFALAMRCLLRLGLLDQAEALLAEALREGPSTELLLLALETAYLRKDVPRL
                     REILSGLDLERLDMGEELRSLLASWKAQA"
     gene            2072..3478
                     /gene="pelF"
                     /locus_tag="VFL04_05205"
     CDS             2072..3478
                     /gene="pelF"
                     /locus_tag="VFL04_05205"
                     /inference="COORDINATES: protein motif:HMM:NF038011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HET7839135.1"
                     /translation="MRVCLVIEGSYPYITGGVSAWVHELIAGLSRIEFALYTLSPVAG
                     QELRYALPPNVVEHRDLVLTASAASRRGRFRRAKVGAILQAHALMVGGGEVDLKAFMA
                     AIPEGRGIHRDAILDTRAWRYIGERNRIHNPLYPFADYFWAWKSAHDLIFNVLASEAP
                     AADVYHSLSTGFAGLAALAAKVRRGAPFLLTEHGLYHKEREIEIRKASFVKGYQRDMW
                     TRLYNRIAELCYRNAELCTSLFEDNRGHQLGLGATRERTVVIPNGIDVERYSVQRQRR
                     DEFFNVGFVGRIVPIKDVKTFLVAAKLVLEAEPRARFHLIGPEDEDKGYAAECRRLAA
                     DLRIDDRVDFVGRHDVRDYYRFLDVVVLTSVREAQPLVILEAYAAGIPVVSTDVGNVA
                     ELLEGDPRFIAPVKDSAAIAEGIVYVARHAEEMRELAKRNRARVLASYDKKELLLRYG
                     ELYARLAPLSGRSPPGRA"
     gene            3482..>4770
                     /gene="pelG"
                     /locus_tag="VFL04_05210"
     CDS             3482..>4770
                     /gene="pelG"
                     /locus_tag="VFL04_05210"
                     /inference="COORDINATES: protein motif:HMM:NF028243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HET7839136.1"
                     /translation="MAGIGFELRRLVETRTISGFAGAAFSGVVIVAGPWILTILSLSA
                     AQSLPFFAEGEVALAFTGAMVWTLAASLCLSAGFLHIFVRLSADLIYEGKRAEAACLL
                     VKVAAATAAVSLPIGWALASLLIANGAHAMVFRLGFALLLAAANVLWAAMMTVTAIKR
                     YGLVLAAYAAGMGLMYWLAALLGPALGAAGALLGLAAGYAATAIILIAAALGGLGLAP
                     LPGAAARLAAYGRRYRHLALTGAAYALGTWIDKFLLWGVRGSRAEGTWFSVYPPYDNA
                     FFLANLALIPGLIYFTISTETGFSLDLRRFLGFLAHRRRPEVEAARRRLGRSARSALA
                     AQSGFQAVVSVALSLLGPYITACLGAPAGAFLLLLWGGFFQLILLTALNMLFYLELYA
                     AAAITGLCFLGVNALISGLIAFGPAPIPLGLPYLAAG"
BASE COUNT          639 a         1866 c         1499 g          766 t
ORIGIN      
        1 ggcctcctcc tcccggcctt ccatggcggc ccagccatgg gctacctccc ccagaccctg
       61 ggctccgtcg tcgtggcctc cctcctcggg gcggggcccg gcatcctcgc cctcgccgcc
      121 gccgccggcg ccaccgccct cctgcccctc gcgggcgagc tcctcgagac ggcccgtctg
      181 ccagcggccg ccgccctcct cggcgtcctc gccgcgggca cggtgcgcga cgccgctgag
      241 gctagggagc gcaggctcat ggagaggatc agggacagcg tgaagcgggg cgagcgcttc
      301 tcgaggatga gcgacaccct catccgtctc aacgaggagc tcgaggcgag ggtctcgggc
      361 cagagggagt cggtgtcgac cctctacgcc cgcatcagga agctggacag cctcgagctc
      421 aagactgtcc tcggggccct gctcgaaggg gtgagcgcct tctcccaggc cgagaaggcc
      481 gcggtgtacg agtacgacgc cgcagcgggg accctcgtcc tccaggcctg gatcggggcc
      541 aagcccgagc ccagcctcga gctcgccggc agggtcgagg gctgggtctt ccgcaacgac
      601 cgccccttca gcctcaggat ggtcggctcc gagctcggct tcgaccgcct cgactcgagg
      661 cggagcgtcc tcgcctaccc ccttaagtca ggcgcgcagg cctggggcgt gctcaacatc
      721 gaggagatgc ccttctaccg ctacaacccc acgacagaga agaacctcga aatcatcgtc
      781 agcctctcgg cggcctatat cgggagggcc gtcgacttcc gcgagcgggt cctcaagcgg
      841 ccgcgaaacg aggtcaccgg cctgcccggc tacggggagc tcgtccgcct cctcggcgag
      901 gagctcgcga ggcgggacgg gggccacgcc tcggtctccg tcgtcctcgt cgagttcctc
      961 gacttcgagg ccctcgtgtt cgcccactcg ggggtcaagg ccctcggcct cgtcaaggcc
     1021 accgccgtcg agagcgccaa ggcgggccgg gccatcgcct tccactacaa ggaggggagc
     1081 cagctggcct tcatcctgcc cggcaaggac agggacgggg cctcgctctt ctgcctcgag
     1141 ttcgcccaac tcgtcggcag gatggccctc gagatcgacg gggagccggt caggctcgag
     1201 cccgtcttcg gcctcgcggc ggcgctccgg gactccggcg ccgaggccct cctcgccgag
     1261 gccgagcgcc tcctctgcct ctcgcgcgcg tccttcgcgg agcgggtcgg gcccggatcc
     1321 ggcagcgggc catgagcggc ttcgactaca tccgccacct ggccgactac tacctcccca
     1381 agacccccca cgcggccctc ctcgccgtac aatccgacta cctccccctc gaggccttcg
     1441 tctacaagtc ctcgtcggtg aagtccctct tcgaggagcc ctatgacctc gccgagctcg
     1501 accggatcct ggcgaggccg aggctcgagc tcggcgaggc gatgctcctc ggcgagatct
     1561 tctggaccat gacccacggc gatgacaagg agctggccct cttcgctgcc gagagcctgg
     1621 gcgccctcga gaaccgctgg gcgcggagga tcgaggagct cgccgccctg gtggccctcc
     1681 ccggctgcgg ggacggggac tgcttcgcct acgccagggc cctctacgag tacgccctca
     1741 tcgccggcgg gcatggcccg atccggaact actacctccg cgaggccttc tacgccctct
     1801 caaggcacgg cgtggccagg ctcgaggccg agggcttcgc cctcgccatg cgctgccttc
     1861 tgaggctcgg cctcctcgac caggccgagg ccctcctcgc cgaggccctg cgcgaggggc
     1921 ccagcaccga gctcctcctc ctcgccctgg agaccgccta tctccgcaag gacgtccccc
     1981 gcctccggga gatcctctcg ggcctcgacc tcgagcgcct cgacatgggc gaggagctgc
     2041 gcagcctcct cgcctcgtgg aaggcccagg catgagggtc tgcctcgtca tcgagggctc
     2101 ctacccctac atcaccggcg gggtctcggc ctgggtgcac gagctcatcg ccggcctgtc
     2161 ccggatcgag ttcgccctct acaccctctc gcccgtggcc ggacaggagc tccgctacgc
     2221 ccttccgccc aacgtcgtcg agcaccgcga cctcgtcctc acggcgagcg ccgcctcgag
     2281 gcgcgggcgc ttccgccgcg ccaaggtggg cgcgatactc caggcccacg ccctcatggt
     2341 cggaggcggc gaggtcgacc tcaaggcctt catggccgcc atacccgagg gcagggggat
     2401 ccaccgcgac gccatcctcg acacgagggc ctggcgctac atcggggagc gcaaccgcat
     2461 ccataacccc ctctacccct tcgccgacta cttctgggcc tggaagagcg cccacgacct
     2521 tatcttcaac gtcctcgcct ccgaggcccc ggcggccgac gtctaccact cgctctccac
     2581 gggtttcgcc ggcctggccg ccctcgccgc caaggtgcgg cgcggggcgc ccttcctcct
     2641 caccgagcac ggcctctacc acaaggagag ggagatcgag atccgcaagg ccagcttcgt
     2701 gaagggctac cagcgcgaca tgtggacgag gctctacaac cgcatcgccg agctctgcta
     2761 ccgcaacgcc gagctctgca cctcgctctt tgaggacaac cgcggccacc agctcggcct
     2821 cggcgccacg agggagagga ccgtggtcat cccgaacggg atcgacgtcg agcgctactc
     2881 ggtccagcgg cagaggcggg acgagttctt caacgtcggc ttcgtgggcc gcatcgtgcc
     2941 catcaaggac gtgaagacct tcctcgtggc cgccaagctc gtgctcgagg ccgagccgag
     3001 ggcccgcttc cacctcatcg ggcccgagga cgaggacaag ggctacgccg ccgagtgcag
     3061 gcgcctcgcc gccgacctca ggatcgacga ccgggtggac ttcgtcggcc ggcacgatgt
     3121 gcgcgactac taccgcttcc tcgatgtcgt cgtgcttacg agcgtgcgcg aggcccagcc
     3181 cctggtcata ctggaggcct acgccgccgg catacccgtg gtgtcgaccg acgtcgggaa
     3241 cgtggccgag ctcctcgagg gcgatccccg cttcattgcc ccggtcaagg actccgcggc
     3301 catagccgag ggcatcgtct acgtcgccag gcacgccgag gagatgcgcg aactcgcgaa
     3361 gaggaaccgc gccagggtcc tcgcctccta cgacaagaag gagctcctcc tccgctacgg
     3421 cgagctctat gcccgcctcg ccccgctctc gggccgctcg cccccaggga gggcctaggc
     3481 catggcgggc ataggcttcg agctgcgccg cctcgtcgag acgaggacga taagcggctt
     3541 cgcgggcgcc gccttctcgg gcgtcgtcat cgtggccggg ccatggatcc tcaccatcct
     3601 gagcctctcg gccgcccaga gcctcccctt cttcgccgag ggcgaggtgg ccctcgcctt
     3661 cacgggcgcc atggtctgga ccctcgcggc ctcgctctgc ctgagcgcgg gcttcctcca
     3721 catcttcgtc aggctttcgg ccgacctcat ctacgagggg aagagggccg aggccgcctg
     3781 tctcctcgtc aaggtcgcgg cggccacggc ggcagtctcg cttcccatcg gctgggctct
     3841 cgcctccctc ctcatcgcca atggcgccca cgccatggtc ttccgcctcg gctttgccct
     3901 cctcctcgcg gcggccaacg tgctttgggc cgcgatgatg acggtgacgg ccataaagcg
     3961 ctacggcctc gtgctcgccg cctacgcggc ggggatgggc ctcatgtact ggctcgcggc
     4021 cctcctgggc ccggccttgg gcgccgcggg cgccctcctc ggccttgcgg ccggctatgc
     4081 cgccacggcc atcatcctca tcgccgcggc cctgggcggc ctcggcctgg cgcccttacc
     4141 cggggccgcc gcgcgcctgg ccgcctatgg ccggcgctac cgccacctgg ccctcacggg
     4201 ggctgcctac gcccttggca cctggatcga caagttcctg ctctgggggg tccgcggcag
     4261 ccgggccgag gggacctggt tctcggtcta ccccccctac gacaacgcct tcttcctcgc
     4321 gaacctcgcc ctgatcccgg gcctcatcta cttcaccatc tccacggaga cgggcttcag
     4381 cctcgacctg cgccgcttcc tcggcttcct cgcccaccgg cgcagacccg aggtcgaggc
     4441 tgcacgccgc cggcttggcc gctcggcgcg ttccgccctg gcggctcagt cgggcttcca
     4501 ggccgtggtc tcggtggcgc tcagcctcct cgggccctac atcaccgcct gcctcggggc
     4561 cccggccggg gcatttctcc tcctcctctg gggaggcttt ttccagctca tcctcctcac
     4621 ggccctcaac atgctcttct acctcgagct ctacgccgcg gccgccatca cggggctctg
     4681 cttcctcggc gtgaatgccc tcatctcggg cctcatcgcc ttcggccccg cgcccattcc
     4741 cctcggcctc ccctacctgg ccgcgggctt
//
LOCUS       DASSQV010000302         6075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2060243, whole genome shotgun sequence.
ACCESSION   DASSQV010000302 DASSQV010000000
VERSION     DASSQV010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060243"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /gene="htpG"
                     /locus_tag="VFL04_05215"
     CDS             <1..1447
                     /gene="htpG"
                     /locus_tag="VFL04_05215"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET7839137.1"
                     /translation="SRRAGKDGAFRWSSDGKGEYAIEPAERVGQGTEVRVHLNDEGLE
                     YLKRWKTEPVIKKYSNHIAYPITLVSEEPDYDDKGAKKGTKHVEEVVNAASALWRRNK
                     AELKDEDYAEFYKSFTGEEEAPLHWIHTHAEGTIEYTTLFYFPSKAPFDLYRADYKSG
                     VKLYVKRVFITDDEKELLPVYLRFVRGIIDSEDLPLNVSREILQQNRVMTNIRTGSVK
                     RILGELADMAKLNPDKYAKFIAEFNRPLKEGLYSDYANRDALLGLVRFKSSSVEGWTG
                     FADYKARMKEGQKAIYYIAGGPEDRLRKSPLLEMYRKEGIEILICDDEIDELVLSGLG
                     PYESIEIKSVNRSGSESELGERKDESPAGPDAAGAVDKAKKALGDSVKDVRLSRRLDE
                     SPACIVVDSDDPSLQFRELMKQLGGGELPDVKPILELNPSHPLVKRLAAAAEGEAEDL
                     SKVLLGQALLVEGAPLADPADFAARLTRLLA"
     gene            complement(1506..2435)
                     /locus_tag="VFL04_05220"
     CDS             complement(1506..2435)
                     /locus_tag="VFL04_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET7839138.1"
                     /translation="MGKTALLVLAAGMGSRFGGPKQAAPVGPSGETLLEYSIYDALRA
                     GFDELVFLIGREAEPAFRERVLACLPARVPWRLAFQELDSLMDGRAAARARASGRTKP
                     WGTGQALLCAASGLELPFASVNADDFYGSESFRVVHDFLEGGSPGGASWCMAGFALAN
                     TLSPNGPVSRGICSIDEAGLLMGVTEQRQIGAGGPGEGIVAARAEGGSLGLTGRETVS
                     MNLWGLTPSVLGPGLELFGEFLEQNAGSATAEFGLPDMVDALMARGVAKVRVLPTAET
                     WFGLTYRADLAESRSRIEELTRSGSYPSPLWGT"
     gene            2500..3189
                     /locus_tag="VFL04_05225"
     CDS             2500..3189
                     /locus_tag="VFL04_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839139.1"
                     /translation="MDDRGLSPRDFLDLDRSLAAGIFAGIAYPWEAIPRIAGLVSALI
                     AAPPPGYALIAPGLLVAEGCSVSKRSEIIGPAIIGPGCEIRAGALIRENVILGAGCLV
                     GNSSELKNCILFDSALAPHFNYIGDSILGTSAHLGAGVILSNFRSDHGEVKVHLGDGS
                     TIGTGLQKLGAIVGDRAEIGCNSVCFPGSLVGRDSIVYPLCPVRGHVPARVLLRAEGL
                     HEPRRGLPGQG"
     gene            3214..3819
                     /locus_tag="VFL04_05230"
     CDS             3214..3819
                     /locus_tag="VFL04_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7839140.1"
                     /translation="MIKAVVFDFGNVLCKLERGLANAAMAAHTSLSPEEVGLRVWNGS
                     IERDSETGLIDSREHFRRIRVALEAGSAWSYDDFVEEIGRAIVPYPEGEASLQGVAGL
                     GLRCFILSNTNWIHSLKIFGREVLATVPELHALSFKIGAMKPDPRIWLWLLERAGLEA
                     RDCLYVDDVEAYCVAARSLGFSALRFHHSGGNLLQAVERLL"
     gene            3887..4861
                     /locus_tag="VFL04_05235"
     CDS             3887..4861
                     /locus_tag="VFL04_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7839141.1"
                     /translation="MKRLLIVAMCALIALSAYAAPNKVVVYTAHEDAILNLLAPLFQK
                     ETGITLEYVKMASGDIIKRAKAESANPQADVIWSIGGEQLEANSDILAAYTPKEWSKI
                     NPLFKVGTNWLPYTGIMNVFIINTKLVSPDKAPKKWTDLSDPRFAKMISSARADKSGS
                     SYMQLCNVITVYKDKGWDIYKSILKNMAISGSSGAVPKFVNDGEAMVGITLEDNAYRY
                     VKGGGPVQIVYPEDGTVAAPDGLALVKGAPNPDAGKAFIDWCLSKPTQEILVDQMFRR
                     SVRVDCKDPPGLPPIGKLKTVPYDFGWAAKSQGEFTKRFQDMAMDLGL"
     gene            4955..6061
                     /locus_tag="VFL04_05240"
     CDS             4955..6061
                     /locus_tag="VFL04_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7839142.1"
                     /translation="MTSIRIVEIEKRFGTLRALKGVSVAIEPGELFTLLGPSGCGKTT
                     LLRTVAGFNRQDSGDIFLGEELVNDQPAHRRETGMVFQNYAVFPHMTVFDNVAYGLRV
                     RRQGRKEIASKVEAALRRVRLEGLDQRTPDKLSGGQQQRVGLARAMAIEPRVLLLDEP
                     LSNLDAKLRVEMRGEIRDIQKQLGITSIYVTHDQEEALVISDRIAVMESGVIQQVGPP
                     WAIYKDPVNLFVASFVGSMNLIDAEILGEGPGGTVRAAAAGCELLIPAPADSYTSKAI
                     RIAFRPEDAAEPVAGGPANELRATVRTASFLGSAVSLELELGGRIILVERSRPSALDI
                     LKAGSQTSFSVPLDSILLFHPATGDRVPAKRSRG"
BASE COUNT         1070 a         2167 c         1930 g          908 t
ORIGIN      
        1 gtccagacgc gccggcaagg atggggcctt ccgctggtcc agcgacggca agggcgaata
       61 cgccatcgag ccggccgagc gcgtgggcca gggcaccgag gtgagggtcc acctgaacga
      121 cgagggcctc gagtacctca agcgctggaa gaccgagccg gtgatcaaga aatactcgaa
      181 ccacatcgcc taccccatca ccctcgtctc cgaggagccc gactacgacg acaagggcgc
      241 gaagaagggc acgaagcacg tcgaggaggt cgtcaacgcg gcctcggccc tctggcgccg
      301 caacaaggcc gagctcaagg acgaggacta cgccgagttc tacaagtcct tcaccggcga
      361 ggaggaggct cccctccact ggatccacac ccacgccgag ggcacgatcg agtacacgac
      421 cctcttctac ttcccctcca aggccccctt cgacctctac cgggccgact acaagagcgg
      481 ggtgaagctc tacgtcaagc gcgtcttcat caccgacgac gagaaggagc tcctgccggt
      541 ctacctccgc ttcgtgcggg gcatcatcga ctccgaggac ctgccgctca acgtgtcgag
      601 ggagatcctg cagcagaaca gggtcatgac caacatccgc acgggcagcg tcaagcgcat
      661 cctcggcgag ctggccgaca tggccaagct caaccccgac aagtacgcca agttcatcgc
      721 cgagttcaac cgccccctga aggaggggct ctactccgac tacgccaacc gggacgccct
      781 cctcggactc gtccgcttca agagcagctc ggtcgagggc tggaccggct tcgccgacta
      841 caaggcgagg atgaaggagg gccagaaggc gatctactac atcgccggag gccccgagga
      901 caggctccgc aagtcccccc tcctcgagat gtaccgcaag gagggcatcg agatcctcat
      961 ctgcgacgac gagatcgacg agctcgtcct ctcgggcctc ggcccctacg agagcatcga
     1021 gatcaagagc gtgaaccgct cgggcagcga atccgagctc ggcgagagga aggacgagag
     1081 ccccgccggc cccgacgcgg ccggcgcggt cgacaaggcc aaaaaggccc tcggggattc
     1141 ggtcaaggac gtcaggctat cccggcggct cgacgagagc ccggcctgca tcgtcgtcga
     1201 ttcggacgac ccctcgctcc agttccgcga gctcatgaag cagctcggcg ggggggagct
     1261 ccccgacgtg aagcccatcc tcgagctgaa cccctcccat cccctggtca agcgcctcgc
     1321 ggccgcggcc gagggcgagg ccgaggacct ctccaaggtc ctcctcggcc aggccctcct
     1381 cgtggagggc gcccccctgg ccgaccccgc cgacttcgcg gctcggctca cgaggcttct
     1441 ggcctagacc gcggcttttg gcgaggccgg ggcgcccgag gccggatgcg gccttgggcg
     1501 ccctttcatg tcccccacag agggcttgga taggagccgg atcgggtgag ctcttcgatg
     1561 cgggacctcg attcggcgag gtcggccctg taggtgaggc cgaaccaggt ctctgccgtc
     1621 ggaagcaccc tcacctttgc caccccgcgg gccatgaggg cgtcgaccat gtcgggcagg
     1681 ccgaactcgg cggtggccga gcccgcgttt tgttcgagga actcgccgaa gagctcgagc
     1741 cccggaccga ggacgctcgg cgtaaggccc cagaggttca tggagaccgt ctccctgccc
     1801 gtgaggccca ggcttccgcc ctcggcccgg gcggcgacga tgccctctcc cgggccgccc
     1861 gcaccgatct gcctctgctc ggtcaccccc atgaggaggc cggcttcgtc gatggagcat
     1921 atcccgcggg agacggggcc gttggggctc agggtgtttg cgagggcgaa acccgccatg
     1981 caccaggagg ccccaccggg cgagcccccc tcgaggaagt cgtggacgac gcggaaggac
     2041 tcggatccat agaagtcgtc ggcgttcacc gaggcgaagg ggagctcgag cccggaggcg
     2101 gcgcagagga gggcctgtcc cgtgccccag ggcttggtgc ggccgctcgc cctggccctg
     2161 gccgccgccc gcccgtccat gaggctgtcc agctcctgga aggcgaggcg ccacgggacc
     2221 ctggcgggga ggcaggcgag gacgcgttcg cggaaggcgg gctcggcctc tcggcctatg
     2281 aggaacacaa gctcgtcgaa gcccgcgcga agggcgtcgt agatggagta ctcgaggagg
     2341 gtctcgccgc tgggccccac cggggcggcc tgtttgggcc cgccgaacct ggagcccatg
     2401 cccgccgcga gcacgaggag ggctgtcttt cccatgatca gcccatgcta tccgaaatcc
     2461 cgatttgacg cataggtgcg acgcggctat gctggggcga tggacgacag agggctatcc
     2521 ccgcgcgatt tcctcgatct cgaccgaagc ctggccgccg ggatcttcgc gggcatcgcc
     2581 tacccctggg aggcgattcc gcgcatcgcg ggcctggtct ccgccctcat cgccgccccg
     2641 cccccaggct acgccttgat cgctcccggg ctcctcgtgg ccgagggttg cagcgtctcc
     2701 aagcgcagcg agatcatcgg gccggccatc atcggtccgg gctgcgagat cagggcgggg
     2761 gccctcatcc gcgagaacgt catcctcgga gccggctgcc tcgtcggcaa cagctccgaa
     2821 ctcaagaact gcatcctctt cgactcggcc ctggcgcccc acttcaacta catcggcgac
     2881 tcgatcctcg ggacctccgc acacctgggg gcgggggtga tcctctcgaa cttccgctcc
     2941 gaccatggcg aggtcaaggt gcatctcggg gacggctcaa cgatagggac cggccttcag
     3001 aagctcggcg cgatcgtcgg ggacagggcg gagattggct gcaactccgt gtgcttcccc
     3061 ggcagcctcg tgggcaggga ctcgatagtc taccccctct gccccgtccg cggccacgtg
     3121 cccgcccggg tcctgcttag ggccgagggc ctgcacgagc cgagacgcgg cctgcctggc
     3181 cagggctgac ccaatgctac acaaggagag gcgatgataa aggccgtggt cttcgatttc
     3241 gggaacgtgc tctgcaagct cgagcggggc ctggccaacg ccgccatggc cgcccacacc
     3301 tccctcagtc ccgaggaggt gggcctcagg gtgtggaacg gctcgataga gagggactcc
     3361 gagaccggcc tcatcgattc ccgcgagcat ttcaggcgga tccgcgtggc cctcgaggcg
     3421 ggctcggcct ggagctacga cgacttcgtc gaggagatcg gacgcgccat cgtgccctac
     3481 cccgagggcg aggcctcgct ccagggcgtg gccggcctcg gcctcaggtg cttcatcctc
     3541 tccaacacta actggatcca ctcgctgaag atcttcgggc gggaggtgct cgccacggtc
     3601 cccgagctac acgccctctc cttcaagatc ggggcgatga agcccgaccc gcgcatctgg
     3661 ctctggctcc tcgagcgcgc ggggctggag gcccgggatt gcctctatgt cgacgatgtc
     3721 gaggcctact gcgtggcggc gcgttccctg ggcttctccg ccctccgctt ccaccattcc
     3781 ggcggaaatc ttttacaggc ggtcgagagg ctgctataat cggtcgggaa cgggccaggt
     3841 tccggtccgg acacaagtcc ctacaatacg caaacggagg tatccgatga agagactgct
     3901 catcgtggcg atgtgcgcac tcatcgcgct ctctgcctac gcggccccca acaaggtcgt
     3961 cgtctacacg gcccacgagg acgccatcct caacctgctc gcccccctct tccagaagga
     4021 gaccggcatc accctcgagt acgtgaagat ggcctcgggc gacatcatca agcgcgccaa
     4081 ggccgagtcc gcgaatcccc aggccgacgt gatctggagc atcgggggcg agcagctcga
     4141 ggcgaacagc gacatcctcg ccgcctacac gcccaaggag tggtccaaga tcaacccgct
     4201 cttcaaggtg ggcacgaact ggctccccta caccggcatc atgaacgtct tcatcatcaa
     4261 cacgaagctc gtcagccccg acaaggcgcc caagaagtgg accgacctct ccgacccccg
     4321 cttcgccaag atgatctcat cggccagggc cgataagtcg ggctcctcgt acatgcagct
     4381 gtgcaacgtc atcacggtgt acaaggacaa gggctgggac atctacaagt ccatccttaa
     4441 gaacatggcc atctcgggca gctcgggcgc cgtgcccaag ttcgtcaacg acggcgaggc
     4501 gatggtcggc atcaccctcg aggacaacgc ctaccgctac gtcaagggcg gcggccccgt
     4561 ccaaatcgtc taccccgagg acggcaccgt ggccgccccc gacggcctgg ccctcgtgaa
     4621 gggcgcgccc aaccccgacg ccggcaaggc cttcatcgac tggtgtctgt ccaagccgac
     4681 ccaggagatc ctcgtcgacc agatgttccg ccgctcggtc cgcgtcgact gcaaggaccc
     4741 gcccgggctt ccgccgatcg gcaagctcaa gaccgtcccc tacgacttcg gctgggccgc
     4801 caagagccag ggcgagttca ccaagcgctt ccaggacatg gcgatggacc tcggcctcta
     4861 ggccgccagc tcacaagaca gcacaagcgc ggaggctggg atcgggcccc tcggggctcc
     4921 ctcgcctccg cgcttcggag actcgaggaa caccatgaca tcgatcagga tcgtcgagat
     4981 cgagaagcgc ttcggcaccc ttcgcgcgct caagggtgtg agcgtcgcca ttgaacccgg
     5041 cgagctcttc accctgctcg gcccctcggg ctgcggaaag acgaccctgc tccgcaccgt
     5101 cgcgggcttc aaccgccagg actcgggcga catcttcctc ggcgaggagc tcgtcaacga
     5161 ccagccggcc catcgccgcg agaccggcat ggtgttccag aactacgcgg tgttccccca
     5221 catgacggtc ttcgacaacg tggcctacgg cctccgcgtc cggaggcagg gcaggaagga
     5281 gatcgcctcc aaggtcgagg ccgccctcag acgggtcagg ctcgagggcc tcgaccagag
     5341 gacccctgac aagctctcgg gcggccagca gcagcgggtg ggcctcgcgc gggccatggc
     5401 catcgagccg cgcgtcctcc tcctcgacga gcccctctcg aacctcgacg ccaagctcag
     5461 ggtcgagatg cggggcgaga tacgcgacat ccagaagcag ctgggcataa cctcgatcta
     5521 cgtcacccac gaccaggagg aggccctcgt catctccgac aggatcgcgg tcatggagag
     5581 cggcgtgatc cagcaggtgg gcccgccctg ggccatctac aaggaccccg tgaacctctt
     5641 cgtcgcctcc ttcgtcggct cgatgaacct catcgacgcc gagatcctgg gcgagggtcc
     5701 cggaggcacg gtcagggccg cggccgccgg ctgcgagctc ctcatccccg ctccggccga
     5761 ctcctacacc tcgaaggcca tccgcatcgc cttcagaccc gaggacgccg ccgagcccgt
     5821 cgccgggggc ccggccaacg agctcagggc cacggtcagg acagcctcct tcctcggctc
     5881 ggccgtctcc ctcgagctcg agctcggcgg ccggatcatc ctcgtcgagc gctcccgccc
     5941 gagcgctctc gacatcctga aggccggaag ccagacctcc ttctcggtcc ccctcgactc
     6001 gatcctcctc ttccaccccg cgacggggga tcgcgtgccc gcgaaaagga gccgcgggtg
     6061 agccggcgca ggccc
//
LOCUS       DASSQV010000303         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1686325, whole genome shotgun sequence.
ACCESSION   DASSQV010000303 DASSQV010000000
VERSION     DASSQV010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686325"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VFL04_05245"
     CDS             complement(<1..1083)
                     /locus_tag="VFL04_05245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET7839143.1"
                     /translation="MMPASSRPTKLHLAVLLVLAAVAMPAAARSARSPDPLPSIEKSF
                     GVEAPPVTSARSVVILDQATGTVLYAKNPDLVIPPASLTKLITLHLVYEEIKAGRLSK
                     DELVVIDKRDCSPYIPYGSSLMYLRPGMKVSVLDLMKGAAVVSGNDAAFTLARRIAGS
                     NEAFAARMNQAVRALGFHDLHFVEPSGLSELNRATALEYAEFCRIYLSLHPESIAELH
                     SLHYIEFPRAEHATADFKPEGRIIQYNRNNLVLSYDGCDGLKTGYIIESGYNLAATAR
                     RGDTRFIVVTLGGSGEGSAGGGAEQRSTDGAALFDWAFASFITLKPEVGPLNPPRVWF
                     GKDLRAAIVPSTPLAITAPLSAVAGLS"
     gene            complement(1087..>2133)
                     /gene="asnS"
                     /locus_tag="VFL04_05250"
     CDS             complement(1087..>2133)
                     /gene="asnS"
                     /locus_tag="VFL04_05250"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET7839144.1"
                     /translation="LEFLREIAHLRPRTNTFGAVARVRNRMAFAVHQFFQERGFQYVH
                     TPLITASDAEGAGAMFQVTTLDLERIAKSGKPVDYAADFFGKPAFLTVSGQLNVETYC
                     QALGRVYTFGPTFRAENSNTTRHLSEFWMIEPEVAFNELEDNMALAEDFIRSLAKVAL
                     NDCAEDLAFFDQRIQNGLVESLSKVVASSFARMTYTQAITELEKTPESFEFKPYWGCD
                     LQSEHEKFLTEKIVGGPVIVTDYPKDIKAFYMKLNDDGKTVRAMDVLVPRFGEIIGGS
                     EREERLDFLEKRIEGMGLKKETYSWYLDLRRFGTTPHSGFGLGFERLLMYVTGMTNIR
                     DVIPFPRAPKQADF"
BASE COUNT          379 a          614 c          765 g          375 t
ORIGIN      
        1 gctgagcccg gctaccgccg agagaggggc cgtgatagcc agaggagtag agggcacgat
       61 ggccgctctc aggtccttgc cgaaccacac gcggggggga ttgaggggcc cgacctcggg
      121 tttgagcgtg atgaaagaag cgaaggccca atcaaagagg gcggccccgt cggtggagcg
      181 ctgctcggcg ccgccgcccg cgctcccctc gcccgagccg ccgagggtga ccacgatgaa
      241 gcgcgtgtcc ccccgtctcg cggtcgcggc gaggttgtag cccgactcga tgatgtaccc
      301 cgtcttaagc ccgtcgcagc cgtcgtagga gagcacgagg ttgttgcggt tgtactggat
      361 gatccgcccc tccggcttga aatccgcggt cgcgtgctcg gcccggggga actcgatgta
      421 gtggagcgag tgaagctcgg cgatcgactc gggatggagg ctcaggtaga tccggcagaa
      481 ctcggcgtac tcgagggccg tggcgcgatt gagctcggag agcccggagg gctcgacgaa
      541 gtggaggtcg tggaaaccca gggctcggac cgcctgattc atccgcgcgg cgaaagcctc
      601 gttcgagccg gcgatacgcc gcgcgagggt gaaggccgca tcgttgccgg acacgacggc
      661 cgcccccttc atgagatcga ggacgctcac cttcatgccc ggccgcaggt acatgagcga
      721 ggagccgtag gggatgtagg gcgagcagtc gcgtttgtcg atcacgacga gctcgtcctt
      781 ggagagccgc cccgccttga tctcctcgta aacgaggtgg agggtgatga gcttggtcag
      841 cgaggcgggg gggatcacga ggtcggggtt cttcgcgtag agcaccgtgc ccgtcgcctg
      901 gtcgaggatg acgaccgagc gggcgctcgt gaccggtggg gcctcgacgc cgaaggattt
      961 ctcgatggag gggagcgggt ccggagacct cgcagacctc gccgctgccg gcatcgcgac
     1021 cgcggcgagg acgaggagga cggcgaggtg aagcttggta ggtcgagacg aagctggcat
     1081 catgtcctag aaatccgcct gtttgggggc tcgggggaag gggatcacgt cgcggatatt
     1141 ggtcatcccc gtaacgtaca tcaaaagccg ctcgaaaccg aggccaaagc cggaatgcgg
     1201 cgtggtgccg aagcgccgaa gatcgaggta ccaggagtag gtctccttct tgaggcccat
     1261 gccctcgatc cgcttctcga ggaaatccag cctctcttcg cgctcggaac cgccgatgat
     1321 ctcgccgaaa cggggaacga ggacgtccat cgcccgcacc gtcttgccgt cgtcgttgag
     1381 cttcatgtag aaggccttga tgtccttggg gtagtccgtg acgatgacgg ggccgccgac
     1441 gatcttttcg gtgaggaact tctcgtgttc actctgcagg tcacagcccc aatagggctt
     1501 gaactcgaag ctttcggggg tcttctccag ctccgtgatg gcctgggtgt aggtcatccg
     1561 agcgaaggac gaggccacga ccttggacag ggactcgacg agcccgtttt ggatccgctg
     1621 atcgaagaag gcgaggtctt cggcgcaatc gttcaacgcg accttggcga ggctccgaat
     1681 gaagtcctcg gccagggcca tgttgtcctc gagctcgttg aaggcgacct cgggctcgat
     1741 catccagaac tcggagaggt ggcgggtggt gttcgaattt tccgccctga aggtgggccc
     1801 gaaggtgtac acccggccga gggcctggca gtaggtctcg acgttgagct ggccggatac
     1861 ggtgaggaag gccggcttgc cgaagaaatc ggcggcgtag tccaccggct tgccgctctt
     1921 cgcgatgcgc tcgagatcga gggtggtgac ctggaacatc gcgcccgcgc cttctgcgtc
     1981 cgaggcggtg atgagggggg tgtggacgta ttggaagccg cgctcctgga agaattgatg
     2041 caccgcgaag gccatgcggt tgcgcacgcg ggccaccgcc ccgaaggtgt tggtcctcgg
     2101 cctcaagtgg gctatctcgc ggaggaactc gag
//
LOCUS       DASSQV010000304         8355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_444720, whole genome shotgun sequence.
ACCESSION   DASSQV010000304 DASSQV010000000
VERSION     DASSQV010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444720"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFL04_05255"
     CDS             <1..394
                     /locus_tag="VFL04_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839145.1"
                     /translation="WTSAQGRVAATTGDLDALRALLARTTADSALSEDPVFAAFAKGL
                     PGKVVSVGSFSTRRLASMVAGLAASAQAKAGPKIDPSRFSAWYGYLSTGAGDGAAGSW
                     LEGGVFIPSGDAAALIDLAGPILGRQGQ"
     gene            404..1231
                     /gene="mazG"
                     /locus_tag="VFL04_05260"
     CDS             404..1231
                     /gene="mazG"
                     /locus_tag="VFL04_05260"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET7839146.1"
                     /translation="MADAGRAAEAFGRLYSIVARLRAPDGCPWDREQTPKTMRGSLIE
                     EAYELVEAIDEDDGAHVREEVGDVFLLAAMVAYMNEESGAFSVADSLDGISDKLVRRH
                     PHVFGEAEAADSEAVLAQWQEIKEKVEGRRRKDSILDEVSRALPPLERAYRIQKKAAK
                     VGFDWAGIGEVWEKAREELDEAEAACIRAAEAGDREALEEELGDFLFSAVNLARFLKV
                     DPAVALHGPVEKFSRRFRHVEKRMAESGLALGAPHMEKMDGYWNEAKRIEAGSGEGS"
     gene            complement(1228..2052)
                     /locus_tag="VFL04_05265"
     CDS             complement(1228..2052)
                     /locus_tag="VFL04_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839147.1"
                     /translation="MIRIGLSSQALLMRSPAEILSAARSAGAEAREWADGVHVPHDDH
                     KAAEQVMIETLRAGLTTASYSAIHLPEADGGSEARTESVFMAADILQAPNIRLFLGGH
                     PTPRPDPSRRSRLVADARRLGDRAAKSGRTICLSMGSHTFLDRYAEALALTAEIGHPY
                     VRLAWDALPGVNAEEASGALEEAGSSVCLVMARHVERDGIARGLAGEEAQWRRRLAAF
                     KRTELDPKMGLFVFVGAMRDGASGAEGAQACLAEDISLLRRIADELEGGAHRKPGA"
     gene            2227..4632
                     /locus_tag="VFL04_05270"
     CDS             2227..4632
                     /locus_tag="VFL04_05270"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET7839148.1"
                     /translation="MSQLSPAQLRRARKSFFVFNGLNSFSFILLSGSFITLYALRLGA
                     SNALVGALNAFGYITFFFLPLGKRLVKGMPIVKVFGWAWAIRYFAMLPALAAPILVAA
                     GWPGPAFGLILAGVAGFNVARGVGLIGNNPVIAHLADGGKAGRRSDRGTFLVNLSIVS
                     SLTGMAANLGVALILGKTASPELYAVTMGFGIAVGLSGSALLFKAPEPEDYRDDSSTS
                     LWKNAREAMRDAPFRSFIGVFLVLSFASGMARSFLPVYAKEVFGQGDDAVMAYSLVAS
                     VGALAMGLLMRLLVDRQGAKPLYIIFTAISALSLAPLAIAGDATMAQGGILSLPLGAA
                     LLLGFVNFFSSFGFQGEETAGQSYFFALVKRERMLDLGVVYFLVYGIGGSLGAEAGGV
                     ILDLLGLAGFDHVSAYRIFYGFLFLVLVWVLWRMGRLVRLGSASVRESLEIMFSIRDL
                     RTINLLSRLDRSGDPVEQVRLIQEVGSSASGRAQHELLPYLSSPRFDVRMEGLLALEK
                     LRELGDEVEAALVAEVERQPFTTAYVAARILGKTGRTGAIPCLRRAALAQDYMLQGSA
                     MIALARLGDRDSIGSFEELLERTENPRVRISAAYALELLGSRESVPILIACLRRREVA
                     AFVSDELVLSTAAILGLMPRFYAAYTAFLAEEGAGVALLLDAAAEPTAPGLAAETWTL
                     EDYRRALDAILADPADGAPMSRILLADCREGGPPEPGAQTQGQAGARPPTQGGAWQGP
                     ERRQRPRISPEPSGIDFVLAETALDPDIGYRGFRFFIAAYAALRARNPQSSAGGTARP
                     ELH"
     gene            4638..5279
                     /locus_tag="VFL04_05275"
     CDS             4638..5279
                     /locus_tag="VFL04_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7839149.1"
                     /translation="MATENIEVFAAGKAVFFEGEPADCMYILKEGAVELKKQTEKGEV
                     ILKTIATPNEFFGEMALIDGKPRSTSAITVMETRLILVDKTVFDSLLRTNGDFAVKIV
                     KVLSARIRRTNVQLADTVDTRPMERIARGIADYSIRFGDRASGDARYVSKDELKTWLN
                     GHIGAAREEIDAVIYRLKDAKRLADAETKQSKGEYLVISGDFIREHDRRREEN"
     gene            complement(5410..5706)
                     /locus_tag="VFL04_05280"
     CDS             complement(5410..5706)
                     /locus_tag="VFL04_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HET7839150.1"
                     /translation="MLTSKQRSRLAGIAQTRDPLVSLGKAGPSEGLTARLDALLSIHE
                     LVKLRFGVMKESKQELAAQLAQDTSSELVRLVGNVAVFWRRNPDPEQRKIDLDV"
     gene            complement(5720..6313)
                     /locus_tag="VFL04_05285"
     CDS             complement(5720..6313)
                     /locus_tag="VFL04_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET7839151.1"
                     /translation="MVLSIVARILSAAVSIYMLMCTVRILMTWVPGIDTGRGGELLRS
                     TTDPYLRFFSRFRLMRSGKFDFSPIVALAVLAVANQLFATLAYTGRVTLGIALGLVVG
                     AAWSAVAFVLSFVAVCALVRVIAYAARWNSLHPLWMVVDSMLNPILYRINRTIYRGRI
                     VNYLQGLVTGFIVLIALRVAGGGLVNLIIRLLDQLPI"
     gene            complement(6321..7520)
                     /locus_tag="VFL04_05290"
     CDS             complement(6321..7520)
                     /locus_tag="VFL04_05290"
                     /inference="COORDINATES: protein motif:HMM:NF015634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HET7839152.1"
                     /translation="MAGAQLIPDAYQAASSFLNPDPHAGLTTFRSLLIPMGGLSEGMG
                     TAYTAVAKDSSYFEANPAASSSLDYTELAVYHNNWIADTRIESAVYTIRWHDFGFGVG
                     GKWLYLPFTAYDDFGGRTGTGYYSEAMTGFNVSYNFLPGYYFNGLAFGVTGKLAYRSV
                     PVLKNIDTSGQNAVALMLDGGFLTRFNFLKLESWRGKNFSVGMAIKNVGPPVLGDPLP
                     TVASFGLGYSPYRAMVFSLDVSKPINLQNPAVSEGFAFAGGALVDVTKFFRLQGGLLL
                     KGGNPRISIGSSFDVELVRLSVNYTLDLTTQLTPLNRISVQASFLLGDLGRAELAKKV
                     DSLYLSGLEAYAQGNTASAIALWTQALEVDPTFDPARESLRAAQGSADLQATMESLQQ
                     LEPQNGK"
     gene            7775..>8355
                     /locus_tag="VFL04_05295"
     CDS             7775..>8355
                     /locus_tag="VFL04_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839153.1"
                     /translation="MRLKTPCLLLAIVLLAGSAPLGFALGTREDPIAQADALIKAQQY
                     DDAIAYLLGFIKKYPDRFDAAQKRIQSIILRREEYTNAAKSLLAYIRENPDDDAEILR
                     RLQELERLDKYANKTTVVTLKQTRLTIKNQKGLLDIMARGRSLLDRGSWLEASRLYLS
                     GFASLDDSSLFWPEFRDAGYDRMTVSAVSGLVE"
BASE COUNT         1322 a         2881 c         2876 g         1276 t
ORIGIN      
        1 ctggaccagc gcccagggcc gggtcgcggc gacgacgggc gacctcgacg ccctgcgggc
       61 cctcctcgcg cggaccacgg ccgacagtgc cctgagcgag gacccggtct tcgccgcctt
      121 cgcgaagggc ctcccgggca aggtggtctc ggtgggatcc ttctccacgc gacgcctcgc
      181 ctccatggtg gccggcctcg cggcctcggc ccaggccaag gccggcccca agatcgaccc
      241 atcgcgcttc tcggcctggt acggctatct ctcgaccggg gcgggggacg gggccgccgg
      301 ctcctggctc gaggggggcg tcttcatccc ctcgggggac gccgcggccc tcattgacct
      361 ggccggcccc atcctcggcc ggcaagggca gtaggaggac gcgatggctg acgcaggaag
      421 ggcggccgag gccttcggcc ggctctactc gatcgtggcg aggctccgcg cccccgacgg
      481 ctgcccatgg gacagggagc agacgcccaa gacgatgcgc ggcagcctca tcgaggaggc
      541 ctacgagctc gtcgaggcca tcgacgagga cgacggcgcc cacgtcaggg aggaggtggg
      601 cgatgtcttc ctcctggcgg ccatggtcgc ctacatgaac gaggagtcgg gcgctttctc
      661 ggtcgccgac tcgcttgatg gcatctcgga caagcttgtc aggcgccacc cccacgtctt
      721 cggcgaggcc gaggccgcgg actcggaggc cgtcctcgcg cagtggcagg agatcaagga
      781 gaaggtcgag ggtcggagga ggaaggactc catcctcgac gaggtctcgc gcgccctgcc
      841 cccccttgag cgggcctaca ggatccagaa aaaggcggcg aaggtcggct tcgactgggc
      901 ggggatcggc gaggtctggg agaaggcgcg cgaggagctc gacgaggccg aggccgcctg
      961 catcagggcc gccgaggccg gggaccgcga ggccctcgag gaggagctgg gcgacttcct
     1021 cttcagcgcc gtcaacctcg cccgcttcct caaggtcgac cccgccgtgg ccctccacgg
     1081 gccggtcgag aagttctcga ggcgcttccg ccacgtcgag aagcgcatgg ccgagtcggg
     1141 cctggccctc ggcgccccgc acatggagaa gatggacggc tactggaacg aggcgaaacg
     1201 gatcgaggcg ggctcgggcg aggggagcta ggcgcccggc ttccggtggg ccccgccctc
     1261 cagctcgtcg gcgatgcgcc tgagcaggga gatgtcctcg gccagacagg cctgggctcc
     1321 ctcggcgccc gaagctccgt cgcgcatcgc gccgacgaat acgaagagcc ccatcttcgg
     1381 gtcgagctcg gtccgcttga aggcggcgag ccggcgccgc cactgggcct cctcgccggc
     1441 caggccccgc gcgatcccgt ccctctcgac atggcgggcc atgacgaggc agacgctgga
     1501 gccggcctcc tcgagggccc cgctcgcctc ctcggcgttt accccgggca gggcgtccca
     1561 ggccagcctg acgtagggat ggccgatctc ggccgtgagc gccagggcct cggcatagcg
     1621 gtcgaggaag gtgtggcttc ccatggagag gcagatggtg cggccgctct tggcggccct
     1681 gtcgccgagg cggcgggcgt cggcgacgag ccgggagcgc cgcgaggggt cgggccgggg
     1741 cgtggggtgg ccgccgagga aaagcctgat gttgggcgcc tggaggatgt cggccgccat
     1801 gaagaccgac tcggtcctgg cctctgagcc gccatcggcc tcggggagat ggatcgccga
     1861 gtaggaggca gtggtgaggc cggcccggag ggtctcgatc atcacctgct cggcggcctt
     1921 gtggtcgtcg tgcggcacgt ggaccccgtc cgcccattcc cgggcctcgg cccccgcgct
     1981 cctggcggcg gagagtatct ccgccgggct ccgcatgaga agggcctggg atgagagtcc
     2041 gatgcggatc atgggcgctc cagaggcgaa cccctcccgg ggatcgcggg atgttctgaa
     2101 ctttcgcgcc agtataccga cgcccggacc cgacctcaag ccggtccgcc cccgcggggc
     2161 gcggcggggg gcgcgcgaag cccgacacgt agcgcgagag ggcccaggcc gcatatactg
     2221 gaccatatgt cccagctctc tccggcacag ctcaggcggg cccgcaaatc cttcttcgtc
     2281 ttcaacgggc tgaactcctt ctccttcatc ctcctgtccg gaagcttcat cacgctctac
     2341 gccctcaggc tcggcgcctc gaacgccctc gtgggtgcgc tcaacgcctt cggctacatc
     2401 accttcttct tcctccccct gggcaaacga ctcgtcaagg gcatgcccat cgtgaaggtc
     2461 ttcggctggg cctgggccat ccgctacttc gcgatgctgc cggccctggc cgcccccatc
     2521 ctcgtcgccg ccggctggcc cggacctgcc ttcggtctca tcctggccgg ggtggccggg
     2581 ttcaacgtgg ccagaggcgt cggcctcatc ggcaacaacc ccgtcatcgc ccatctcgcc
     2641 gacgggggca aggcgggaag acgcagcgac cgcgggacct tcctcgtgaa cctctcgatc
     2701 gtcagctcgc tcaccggcat ggcggcgaac ctcggcgtgg cgctgatcct gggcaagacg
     2761 gcctcgcccg agctctacgc cgtcaccatg ggcttcggca tcgccgtggg cctctcgggc
     2821 tcggccctcc tgttcaaggc ccccgagccc gaggactacc gcgacgactc ctcgaccagc
     2881 ctctggaaga acgcgcgcga ggcgatgagg gacgcgccct tccgctcctt catcggcgtg
     2941 ttcctcgtcc tctccttcgc gagcggcatg gccaggtcct tcctccccgt ctacgccaag
     3001 gaggtcttcg gccagggcga cgacgccgtc atggcctact ccctcgtcgc gagcgtcggg
     3061 gccctcgcca tgggcctcct catgcgcctc ctcgtcgacc gccagggcgc gaagcccctc
     3121 tacatcatct tcaccgccat ctcggcgctc agcctcgccc ccctggccat cgcgggcgac
     3181 gcgaccatgg cccagggcgg catcctctcc ctgccccttg gcgccgccct cctcctgggc
     3241 ttcgtgaact tcttctcctc attcggcttc cagggcgagg agacggcggg ccagtcctac
     3301 ttcttcgccc tcgtcaagcg cgagcgcatg ctcgacctcg gcgtcgtcta cttcctcgtc
     3361 tacggcatcg gcggatccct gggggccgag gcgggaggag tcatcctcga cctcctcggc
     3421 ctcgcgggct tcgaccacgt gtcggcctac cggatcttct acggcttcct cttcctggtc
     3481 ctggtctggg tgctctggcg gatggggagg ctcgtgcggc tcggctcggc ctcggtccgc
     3541 gaatcgctgg agatcatgtt ctccatcagg gacctgcgca ccatcaacct cttgagccgt
     3601 ctggaccgga gcggggaccc cgtggaacag gtgcggctca tccaggaggt cggcagctcg
     3661 gcctcgggca gggcccagca cgagctcctt ccctacctct cctcgccgcg cttcgacgtg
     3721 aggatggagg gcctcctcgc cctcgagaag ctgcgcgagc tcggggacga ggtcgaggcc
     3781 gccctcgtgg ccgaggtcga gcgccagccc ttcaccaccg cctacgtcgc cgcccgcatc
     3841 ctcggcaaga ccggcaggac gggggccata ccctgcctcc gccgcgcggc cctggcccaa
     3901 gactacatgc tccagggctc ggccatgata gccctcgccc ggctcggcga ccgcgacagc
     3961 atcggctcct tcgaggagct cctcgagcgc accgagaacc cgcgcgtgag gatctcggcg
     4021 gcctacgccc ttgagctcct cggcagcagg gaatccgtcc ccatcctcat cgcctgcctc
     4081 cgcaggcgcg aggtcgctgc cttcgtctcg gacgagctcg tgctctcgac ggcggccatc
     4141 ctcgggctga tgcccaggtt ctacgcggcc tacaccgcct tcctcgccga ggagggggcc
     4201 ggggtggcgc tgctcctcga cgccgccgcc gagcccacgg cgcccggcct cgcggccgag
     4261 acctggaccc tcgaggacta tcggcgcgcc ctcgacgcca tcctcgccga ccctgccgac
     4321 ggggcgccca tgagccgcat cctcctcgcc gactgcaggg agggcggccc gcccgagccc
     4381 ggggctcaga ctcagggcca ggccggggcc cggcccccca ctcagggcgg ggcctggcag
     4441 gggccggagc gccgccagag gccgaggatc tcccccgagc ccagcggcat cgacttcgtc
     4501 ctcgccgaga cggccctcga ccccgacatc ggctacaggg gcttcaggtt cttcatcgcc
     4561 gcctacgcag cgctcagggc ccggaatccc cagagctcgg ctggcgggac ggcccggccc
     4621 gagctacact agtggccatg gcgacagaga acatcgaggt cttcgcggcg ggcaaggccg
     4681 tcttcttcga gggcgagccc gccgactgca tgtacatcct caaggagggg gcggtcgagc
     4741 tcaagaaaca gaccgagaag ggcgaggtca tcctcaagac catagccacc cccaacgaat
     4801 tcttcggcga gatggccctg atagacggga agccgcgctc cacctcggcc atcacggtga
     4861 tggagacgag gctcatcctc gtggacaaga ccgtcttcga cagcctcctg cggaccaacg
     4921 gcgacttcgc cgtgaagatc gtcaaggtcc tctcggcccg gatcaggcgc acgaacgtcc
     4981 agctcgccga cactgtcgac acgaggccca tggagcgcat agcccggggg atcgccgact
     5041 actcgatccg cttcggcgac agggcgagcg gcgacgcccg ctacgtctcg aaggacgagc
     5101 tcaagacctg gctcaacggc cacatcggcg cggccaggga ggagatcgac gccgtgatct
     5161 accggctcaa agatgccaag cgtctcgccg acgccgagac caaacagtcc aagggcgagt
     5221 acctcgtcat ctcgggcgac ttcatcaggg agcacgaccg gaggcgggag gagaattagg
     5281 cccgccccgg gccgggcgct gagcgccctg tgccacagcg gcgcggacgc gggcgcccgg
     5341 agcaccagga ctcgggcgcc ggcgcccgga gcaccgggac gcgggcgccg cgcgaggcgg
     5401 gcttaagcct cagacgtcca ggtcgatctt cctctgctcg gggtcgggat tcctccgcca
     5461 gaagaccgcc acgttgccca cgagccgcac gagctcgctc gaggtgtcct gggcgagctg
     5521 ggctgccagc tcctgtttgg actccttcat gacgccgaag cggagcttca cgagctcgtg
     5581 gatggagagg agggcgtcga gcctggccgt gaggccctcg ctcgggccgg ccttgccgag
     5641 gctgaccagg gggtcgcggg tctgggctat gccggcgagc cggcttctct gcttggatgt
     5701 gagcatcggc gtaccaatcc tatatgggga gctggtcgag gagcctgatg atgaggttca
     5761 cgaggccccc tccggcgacc ctgagcgcta tgagcacgat gaagcctgtc acgaggccct
     5821 ggaggtagtt gacgatcctg ccgcggtaga tcgtccggtt gatgcggtag aggatggggt
     5881 tgagcatgga atcgaccacc atccacaggg gatggaggct gttccagcgg gcggcgtagg
     5941 cgatgacgcg gaccagggcg cagaccgcga cgaaggagag cacgaaggcc acggccgacc
     6001 aggccgcgcc caccacgagg cccagggcta tgccgagggt cacgcggccc gtgtaggcca
     6061 gggtggcgaa gagctggttg gcgacggcga gcacggccag ggccacgatc ggcgagaagt
     6121 cgaacttgcc cgagcgcatg agacggaagc gcgagaagaa gcggaggtag gggtcggtcg
     6181 tcgagcgcag gagctcgccg ccccggcccg tgtcgatccc cggcacccag gtcatgagga
     6241 tgcggacggt gcacatgagc atgtagatcg agaccgcggc actcaggatc cgcgcgacga
     6301 tcgaaaggac catgggcgcc tcactttccg ttctggggtt ccagctgctg gagcgactcc
     6361 atcgtcgcct ggaggtcggc cgatccctgg gccgcgcgca ggctctcccg cgcggggtcg
     6421 aaggtcgggt ctacctccag ggcctgggtc cagagcgcga tggcgctcgc cgtgttgccc
     6481 tgggcgtagg cctccaggcc gctaagatac aggctatcga ccttcttcgc aagctcggcc
     6541 ctgccgaggt cgccgaggag gaaggaggcc tggaccgaga tgcggttcag gggcgtgagc
     6601 tgggtggtga ggtcgagggt gtagttgacc gagagccgca cgagctcgac gtcgaatgac
     6661 gagccgatgc tgatcctcgg gttccccccc ttgagcagga ggccgccctg gagcctgaag
     6721 aacttcgtca cgtcgacgag ggccccgccg gcgaaggcga agccctccga gaccgcgggg
     6781 ttctggaggt tgatcggctt ggagacgtcg agggagaaga ccatggccct gtagggcgag
     6841 tagccgagtc cgaaggaggc gaccgtcggc agggggtcgc cgagcacggg gggcccgacg
     6901 ttcttgatcg ccatgcccac cgagaagttc ttgccccgcc aggactcgag cttgaggaag
     6961 ttgaagcggg tgaggaagcc gccgtccagc atgagggcca cggcgttctg cccgctcgtg
     7021 tcgatgttct tgaggacggg caccgagcgg taggccagct ttccggtcac gccgaaggcc
     7081 aggccgttga agtagtagcc gggcaggaaa ttgtaggaga cgttgaagcc cgtcatcgcc
     7141 tcggaatagt agccggtgcc cgtccgcccg ccgaagtcgt cgtaggcggt gaagggcagg
     7201 tagagccatt tgcccccgac gccgaagccg aagtcgtgcc agcggatcgt gtacaccgcg
     7261 ctctcgatcc tcgtgtcggc gatccagttg ttgtggtaga cggccagctc ggtgtagtcg
     7321 aggctgcttg aggcggccgg gttggcctcg aagtaggacg agtccttcgc gacggcggtg
     7381 taggccgtgc ccatgccctc ggacaggccc cccatcggga tgaggaggga gcggaaggtc
     7441 gtgaggccgg cgtggggatc ggggttcagg aaggatgatg ctgcctgata ggcgtcgggt
     7501 atgagctgtg ctcccgccat gaaagcgggt aggacaccca agaccagagc gaggaaatgg
     7561 ttcggccgcc tcatgtcgta aacgaattat actgtgctcg gagggggatt tctatcgggg
     7621 ggcgatcttc ctacaatatg ttgccccggg ggccgaaaat agaggtgaga gccccgtggc
     7681 cccatctgtc cgaagccggg caggagcctg gcgtccgggt cccgccgccc gaagcggggt
     7741 atcatgggag tggaaaggta ttgacctgac acagatgcgc ctgaagacgc cttgcctcct
     7801 cctcgcgatc gtcctcctcg ccggctcggc ccccctcggc ttcgccctcg ggacccgcga
     7861 ggaccccatt gcccaggccg acgccctcat caaggcccag cagtacgacg atgccatagc
     7921 ctacctcctc ggcttcatca agaagtaccc cgacaggttc gacgccgccc agaagcgcat
     7981 ccagtccatc atcctgcgcc gcgaggagta cacgaacgcg gcgaagagcc tcctggccta
     8041 catccgtgag aaccccgacg acgacgccga gatcctgcgg cggctccagg agctcgagcg
     8101 cctcgacaag tacgccaaca agacgacggt cgtcaccctc aagcagacga ggctcaccat
     8161 caagaaccag aagggcctcc tcgacatcat ggcgaggggc aggagcctcc tcgacagggg
     8221 cagctggctc gaggcgagcc ggctctacct cagcggcttc gcctccctcg acgacagctc
     8281 cctcttctgg ccggagttcc gcgacgccgg ctacgacagg atgacggtct cggcggtctc
     8341 gggcctcgtg gagag
//
LOCUS       DASSQV010000305         4051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1314416, whole genome shotgun sequence.
ACCESSION   DASSQV010000305 DASSQV010000000
VERSION     DASSQV010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314416"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFL04_05300"
     CDS             complement(<1..436)
                     /locus_tag="VFL04_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HET7839154.1"
                     /translation="MNYDIAMLGHVSKDVIVDHLGGETRLLGGAVVQSSAAASRSGAR
                     VLVVTKAAAEDIAAVEAMRREGIDIVVGRSAATTSILNVFRTADHELRETSLLSRAEP
                     FAPSDLPADAEVGIVDLAGLFVGELPDSLIEELRPRGRIAVDA"
     gene            complement(441..1112)
                     /locus_tag="VFL04_05305"
     CDS             complement(441..1112)
                     /locus_tag="VFL04_05305"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET7839155.1"
                     /translation="MRRAFPLVTAHSGCMGSPPNGAEHIALAIASGADLVELDIRMAP
                     DGRVVLAHDEDAALEEGILDLDRAFDLLEGEGSPVVNLDAKEPEAALAAALIARARGL
                     EDSVLFSGLGPAETALMRERIPRFRYLLNADSILPASGYGGYEIRAACRMAAEHGCCG
                     LNLEWSSASRELMDYARLRCLPVLLWTVDAEDEMLEALELGPYSLTTNRPDLLMRLLG
                     KDQGR"
     gene            complement(1109..1942)
                     /locus_tag="VFL04_05310"
     CDS             complement(1109..1942)
                     /locus_tag="VFL04_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7839156.1"
                     /translation="MAAYVSRKRGLLFPVLLGLILFISLFPIYWIVVTALKPSIENIS
                     PIPFLLPHRPSLENFASLLAGGYARNLLNSVFVTGASTLLSLALALLAAYALCRHRFP
                     LRLNYAFLVWIILAKMLPPVVLAVPLYETFMRMKLLNSLWGLVLVYQVYTLPYCIWML
                     FGFVKSLPLEFEESAEIDGASRLQVLSRIVVPLVRTGIVATAIFTVIVAWDEFLFAVL
                     FIRSPELLTLPLVIVNFIGEFETLWGQLMAVGLLAALPVLVFSRLVYRHMSSAYALGL
                     K"
     gene            complement(1945..2823)
                     /locus_tag="VFL04_05315"
     CDS             complement(1945..2823)
                     /locus_tag="VFL04_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7839157.1"
                     /translation="MIRETRTRGKALFYWAILAPALALMLWLTVYPMLSVALTSFQDY
                     NYLTDRRSWAGLGNYADLLGDRLFLQSFQNTIVYCLGTTAVEVVLGIVLALVFYGEFR
                     GKRSFMILAIFPMMLSTMVVCAVWKTLYHYDIGLLNYALSSLGLERFGWLIDKGKALA
                     SLALVDIWQWTPFSFIVVQAGLASVPTEIFEAALIDGANGRQRILRIILPILRDQILL
                     LALLRTIDTFKVFAKVYALTGGGPGNATETTSLFIYREAFSYFKFGRSSAASILTLAA
                     VVLMSAFYLRRILRED"
     gene            complement(2893..>4051)
                     /locus_tag="VFL04_05320"
     CDS             complement(2893..>4051)
                     /locus_tag="VFL04_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7839158.1"
                     /translation="DGDYGNAMQVAVDAYTALHPEVSFELLKLSGDDLYQKSVIDLRS
                     GTGTYDLILIDDPKALQFQMAGWLADLGDMYRKAGRAVDPDFIPSLANLCRLPNNPGG
                     KLYGLPFVGNVSLFAYRADLYKKYALPDPKTWSDVLVAAKKISESEEGTNGVTFRGVK
                     GNPIVTVFLPVFWAFGGELLDAKGQPTVNSPEGVKALSYFLELAKYAPKGVAMNNTAQ
                     VRDALLAGTAGIAPEVWPAWLGNMDDPKLSKVAGKVRVMKHPGEVKQSAPMIGIWHIA
                     MPAATRNKDAAFAFLDFVTSRETQKLMAIKAGLPPSRSSVYDDAEVLKLRPWYPAVRD
                     ALVNGKARPRTQVWGEIENVLGDYLQMALIGDLKPEAALNEANAKLVQVLK"
BASE COUNT          694 a         1189 c         1548 g          620 t
ORIGIN      
        1 gggcgtcgac ggcgatgcgg ccgcgggggc ggagctcctc gatcaggctg tcgggcagct
       61 cgcccacgaa aagccccgcg aggtccacga tgccgacctc ggcgtcggcg ggcaggtcgg
      121 agggggcgaa gggctcggcc cgggaaagga ggctggtttc gcggagctca tggtcggccg
      181 tcctgaagac gttgaggatc gaggtggtcg cggcgctcct gcccaccacg atgtcgatcc
      241 cctccctccg catggcctcg accgcggcga tgtcctcggc ggcggccttc gtgacgacga
      301 ggaccctggc ccccgagcgg gaggccgcgg ccgaggactg gaccacagcc ccgccgagga
      361 gcctcgtctc cccgccgagg tggtcgacta tcacgtcctt cgagacgtgg ccgagcatcg
      421 cgatgtcgta gttcactgct tcaccttcct tgatccttcc ctaagagccg catgaggagg
      481 tcggggcggt tcgtcgtcag agaatagggc ccgagctcca aggcctcgag catctcatcc
      541 tcggcgtcga cagtccagag caggaccggc agacagcgca gcctcgcgta gtccatgagc
      601 tcccggctcg ccgagctcca ctcgaggttc aggccgcagc agccgtgctc ggccgccatg
      661 cggcaggcgg ccctgatctc atacccgccg tagcccgagg cgggcaggat ggagtcggcg
      721 ttgaggaggt agcggaagcg ggggatccgc tcgcgcatga gggcggtctc ggcggggccc
      781 aggcccgaga agagcaccga gtcctcgagg cccctggccc gggcgatgag ggccgcggcg
      841 agggcggcct cgggctcctt cgcgtcaagg ttgacgacag ggctcccctc cccctccaag
      901 aggtcgaagg cccggtcgag gtcgaggatg ccctcctcca gggccgcgtc ctcgtcgtgg
      961 gcaaggacga ccctgccgtc cggggccatg cggatgtcga gctcgacgag gtcggcgccc
     1021 gaggcgatgg ccagagcgat gtgctcagcc ccgttgggcg gcgagcccat gcacccggag
     1081 tgggcggtga cgaggggaaa ggccctcctc acttgagccc cagggcgtag gccgagctca
     1141 tgtggcggta cacgagccgg gagaagacca ggacgggcag ggcggccagg aggccgacgg
     1201 ccatgagctg gccccagagg gtctcgaact cgccgatgaa gttcacgatc accaggggca
     1261 gggtcaggag ctcggggctg cggatgaaga ggaccgcgaa gaggaactcg tcccaggcca
     1321 cgatgacggt gaagatggcg gtcgcgacga tgcccgtgcg cacgaggggg accacgatgc
     1381 gcgacaggac ctggagccgc gatgccccgt cgatctcggc cgactcctcg aactcgaggg
     1441 gcaggctctt cacgaagccg aagagcatcc agatgcagta cggcagggta tagacctggt
     1501 agaccaggac gaggccccag aggctgttga ggagcttcat cctcatgaag gtctcgtaga
     1561 gggggaccgc caggacgacg ggcggaagca tcttggccag gatgatccag acgaggaagg
     1621 cgtagttgag ccgcaggggg aagcggtgcc ggcagagggc gtaggccgcg aggagggcga
     1681 gggcgaggga gaggagggtc gaggcccccg tcacgaagac cgagttgagg aggttccggg
     1741 cgtagccccc ggccagaagg ctcgcgaagt tctcgaggct gggccggtgc gggaggagga
     1801 agggtatggg cgagatgttc tcgatcgagg gcttgagggc tgtcacgacg atccagtaga
     1861 tggggaagag gcttatgaag aggatcaggc cgaggaggac ggggaagagg agaccccttt
     1921 ttctcgagac ataggctgcc atggtcagtc ctccctcagg atcctgcgga ggtagaaggc
     1981 cgacatgagg accaccgctg ccagggtcag gatcgaggcc gccgaggagc ggccgaactt
     2041 gaagtaggag aaggcctcgc ggtagatgaa gagcgaggtg gtctcggtgg cgttgccggg
     2101 gcctccgccc gtcagcgcat agaccttcgc gaagaccttg aaggtgtcga tggtccgcag
     2161 gagggccagg aggaggatct ggtcgcggag gatgggcagg atgatgcgga ggatccgctg
     2221 ccggccgttg gccccgtcga tgagggcggc ctcgaagatc tcggtgggga ccgaggcgag
     2281 gccagcctgg accacgatga aggagaaggg ggtccattgc cagatgtcga cgagggccag
     2341 ggaagcgagg gccttgccct tgtcgatgag ccagccgaag cgctcaaggc cgagcgagct
     2401 cagggcgtag ttgaggaggc cgatgtcgta gtggtagagg gtcttccaga cggcgcacac
     2461 gaccatcgtc gagagcatca tcgggaagat ggcgaggatc atgaagctcc gcttgccgcg
     2521 gaactccccg tagaagacga gggcgaggac gatgccaagg acgacctcga cggccgtggt
     2581 gccgaggcag tagacgatgg tgttctggaa ggactggagg aagagccggt ccccgagcag
     2641 gtcggcgtag ttgccgagcc cggcccagct ccttctgtcg gtcaggtagt tgtagtcctg
     2701 gaacgaggtc agggccacgc tgagcatggg gtagaccgtg agccagagca tcagggcgag
     2761 ggccggggca aggatggccc agtagaagag ggccttgccc ctcgttcggg tctcgcggat
     2821 catacgtgcc tctcctgggg atggcggtct gggggagccg ccggcctccg ggccggcggc
     2881 tccggcgctc cgctacttca gcacctggac gagcttggcg ttggcctcgt tcagggccgc
     2941 ctcgggcttg aggtcgccga tgagggccat ctggaggtag tcgccgagga cgttctcgat
     3001 ctcgccccag acctgggtcc ggggtcgggc cttgccgttc acgagggcgt cgcgcacggc
     3061 ggggtaccag ggcctgagct tgaggacctc ggcgtcgtcg tacaccgagg agcggctcgg
     3121 cggaagcccg gccttgatgg ccatgagctt ctgggtctcc cggctcgtga cgaagtcgag
     3181 gaaggcgaag gcggcgtcct tgttcctcgt ggcggcgggc atggcgatgt gccagatccc
     3241 gatcatgggc gccgactgct tgacctcgcc ggggtgtttc atgacgcgca ccttgcccgc
     3301 gaccttggag agcttggggt cgtccatgtt gccgagccag gccggccaga cctcgggggc
     3361 gatgcccgcc gtccccgcga ggagggcgtc gcggacctgg gccgtgttgt tcatcgccac
     3421 gcccttcggg gcgtacttgg ccagctcgag gaagtaggag agggccttga cgccctcggg
     3481 ggagttgacg gtcggctggc ccttcgcgtc caggagctcg ccgccgaagg cccagaatac
     3541 tggcaggaag acggtgacga tggggttgcc cttgaccccc cggaaggtca cgccgttggt
     3601 gccctcctcg ctctcggaga tcttcttcgc cgccacgagg acgtcggacc aggtcttggg
     3661 atcgggaagc gcgtacttct tgtagaggtc ggcgcggtag gcgaacagcg agacgttgcc
     3721 cacgaagggc aggccgtaga gcttgccgcc cgggttgttg ggcaggcggc agagattggc
     3781 gaggctcggg atgaagtcgg ggtcgacggc cctgccagcc ttcctgtaca tgtccccgag
     3841 gtcggcgagc cagccggcca tctggaactg gagggccttg gggtcgtcga tgaggatgag
     3901 gtcataggtc cccgtcccgc tgcggaggtc gatgacggac ttctggtaga ggtcgtcccc
     3961 cgagagcttg aggagctcga agctcacctc ggggtggagg gccgtgtagg cgtcgacggc
     4021 gacctgcatg gcgttgccgt agtcgccgtc g
//
LOCUS       DASSQV010000306         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2309949, whole genome shotgun sequence.
ACCESSION   DASSQV010000306 DASSQV010000000
VERSION     DASSQV010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309949"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..795
                     /locus_tag="VFL04_05325"
     CDS             229..795
                     /locus_tag="VFL04_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839159.1"
                     /translation="MGLGPLGLGEDGRYLRAELGSIEAELQGTKLGELSLGSLKPYRD
                     RLALAAAKDAYVAGLARESFIAPGSGQFRLGEGGSGAAWLTAHLAAVTGGALAFYYLL
                     PSDLRFNHLDYLDASLTDITRAWNSHSVSDYLPAAGAALGGMVLDMALRYLSAKSALE
                     GGREAVDAGKVKLEPLVFPGLLGLGLSY"
     gene            851..1147
                     /locus_tag="VFL04_05330"
     CDS             851..1147
                     /locus_tag="VFL04_05330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HET7839160.1"
                     /translation="MQVTVVSIKVKPERIEAFRAATLENVAASLKEPGVARFELFQDR
                     DDPTHFVLFEAYRDAEGPARHKESSHYLKWKDLAEPMMAEPRTRTFLELLDAGR"
     gene            1149..2045
                     /locus_tag="VFL04_05335"
     CDS             1149..2045
                     /locus_tag="VFL04_05335"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7839161.1"
                     /translation="MNFEFATAARILFGPGSVREVGAAARAFGSRAFVLHGRDPGRAR
                     VLLDLLSAAGLVWEARAVAGEPSFEELAAALAQARAFGAELVLGFGGGSCIDAAKAVA
                     ALLANPGEILDYAEVIGAGKPLTAPSLPCIALPTTAGTGSEVTRNAVLSSAAHRVKVS
                     LRSPTMLPRLAIVDPELARDLPPAATASTGMDALTQLIEPFVSRRANPLTDSLCRDGI
                     GRAFACLPRAFRDGHDAAAREGMALASLESTRTSEPDRASSRGRGGEGLRYEGSRKNP
                     ATAARASDPSLPGRPTIGSPEA"
BASE COUNT          309 a          836 c          790 g          325 t
ORIGIN      
        1 ggatcctaaa gggccccgcc gatccacgct ttcggatcga ggaggcagga catgaaacgt
       61 ttcgcggcac ttctcatcgc gcttttggcc cttctggctt tcggcgccgc cggcttggcc
      121 gcccaaacgg ggccggggtc ctgggacggg tccggggcct gggtacccgg ggcctgggga
      181 tccggggctt ggggatccgg gacttgggcg gggcccggcc accgcggcat gggcctgggc
      241 ccattgggcc tgggcgagga cgggcgctat ctccgggccg agctcgggtc catcgaggcc
      301 gagctccagg ggacgaagct cggcgagctc agcctgggtt cgctgaagcc ctatcgggac
      361 aggctcgcgc ttgccgccgc caaggacgcc tatgtcgcgg gtctggcgag ggagtccttc
      421 atcgcccccg gctcgggcca gttcaggctg ggcgagggag ggtccggggc tgcctggctc
      481 actgcccatc tcgcggcggt cacgggcgga gccttggcct tctactatct cctgccctcc
      541 gacctccgct tcaaccacct cgactacctc gacgcctcgc tcaccgacat cacgagggcc
      601 tggaactcgc acagcgtcag cgactacctg ccggccgcgg gggcagccct cgggggcatg
      661 gtcctcgaca tggccctgcg ctacctctcg gccaagtcgg ccctggaggg cggtcgcgag
      721 gccgtcgacg ccggcaaggt gaagctcgag cccctcgtgt tccccggcct tctgggcctt
      781 ggcctgtcct actagtggcg ctcgccgggg cgaaggcgct atcctcgagg ggcaggaagg
      841 aggccagagc atgcaggtga ccgtcgtgag catcaaggtg aagccggagc ggatcgaggc
      901 cttcagggcc gccaccctcg agaacgtcgc cgccagcctc aaggagccgg gcgtagcgcg
      961 tttcgagctt ttccaggacc gggacgatcc cacccacttc gtgctcttcg aggcctacag
     1021 ggacgccgag ggcccggccc ggcacaagga gagctcccac tacctgaagt ggaaggacct
     1081 ggccgagccc atgatggccg agccgcgcac gcggaccttc ctcgagctgc tggacgccgg
     1141 tcgctagggt gaacttcgag ttcgccacgg ccgcgaggat cctcttcggc cccggctcgg
     1201 tccgcgaggt cggggccgcg gccagggcct tcggcagccg ggccttcgtc ctccacggcc
     1261 gcgacccggg gcgggccagg gtcctcctcg atctgctctc agccgccggc ctcgtctggg
     1321 aggccagggc cgtcgccggc gagccgagct tcgaggagct ggccgccgcc ctggcccagg
     1381 cccgtgcctt cggcgccgag ctcgtcctgg gcttcggcgg gggatcgtgc atcgacgccg
     1441 ccaaggccgt ggccgccctc ctcgccaatc ccggcgagat cctcgactac gccgaggtca
     1501 tcggcgcagg gaagcctctc acggccccct cattgccctg catcgccctg cccaccaccg
     1561 cggggacggg ctccgaggtc acgcgcaacg ccgtcctctc ctcggccgcc caccgcgtca
     1621 aggtgagcct gcgctcgccc acgatgctgc cccgcctcgc catcgtcgac cccgagctgg
     1681 cccgcgacct gccccccgcg gccaccgcca gcacgggcat ggacgccctc acccagctca
     1741 tcgagccctt cgtgtcccgc cgcgcgaacc cacttaccga ctcgctctgc cgcgacggga
     1801 tcggcagggc cttcgcctgt ctgcctcggg ccttccgcga cggccacgac gccgcggccc
     1861 gagagggcat ggccctcgcc agcctcgaga gcacgaggac atctgagccc gacagggcct
     1921 cgagccgggg caggggcggg gaggggctca gatatgaagg ctcgaggaag aatcctgcca
     1981 cggccgcgag ggcatcggat ccgtccctgc cgggcaggcc cacgatcggg agccccgagg
     2041 cgtaggcagc gttgagcctc cggcggtact cctcgttgcg gaccagggga tggcccgcca
     2101 tgagggccgg gaaccctccc gaggccgctg gccccgaggg caaggcgccc ggagccgggc
     2161 aggccagaag ggcgcccgcc gtggccagga gggcgaggcc ataggacatc ggccttccaa
     2221 gccattccgg cagcttgatg cgcgcccgct ccgcggcctt
//
LOCUS       DASSQV010000307         3024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1065755, whole genome shotgun sequence.
ACCESSION   DASSQV010000307 DASSQV010000000
VERSION     DASSQV010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065755"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(462..1904)
                     /locus_tag="VFL04_05340"
     CDS             complement(462..1904)
                     /locus_tag="VFL04_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7839162.1"
                     /translation="MTDATRKPILVVDDDPDVLKRMERLLKFEGFPEVISCSDPRLVP
                     DIMREREISVMVLDLLMPAIPGQRVLQEVRERHPEVPVIVATAVSDLDSAIECMRLGA
                     FDYVPKTAESGRLSASIRHALSIRELQRDYSALKDTLLAARPPQSPAFSKIITRNERM
                     LSVLRYVETVSESPKPILVVGESGTGKELVAEAVHLLSGRAGSLVRVNIAGLDDTMFS
                     DSLFGHRRGAFTGAETDRGGLIERAEGGTLFLDEIGELSPGSQVKLLRLIEDKRYYPL
                     GADLPKATTASLVTATNRDLRAASASGSFRLDLFYRLQTHLIELPPLRERLDDLPLLL
                     SHFAKAAAERLGRSMPIVPDEVSVLLESYEFPGNIRELESMVYDAVSRSKGNTLALDS
                     FREKTRAGGKGEGTRMVAEDGRTRFSGWAKLPTIKDATDELMREALRRAKGNQGIAAD
                     MVGLSRTALNKRLRAGRSAEPGAEDADSGV"
     gene            complement(1901..>3024)
                     /locus_tag="VFL04_05345"
     CDS             complement(1901..>3024)
                     /locus_tag="VFL04_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7839163.1"
                     /translation="EDRGYAVLSQLSSERAACLSVEDPRIDLVLLDASSPRRDGLGVC
                     ERIRKRKSLSELPVIVMTDRDSSESVAEAFRAGASDYLPKLAPTELLFARVDTHVALK
                     RALSEAIETRRRVAEFEKLKTLGVLSAGVAHEINTPNNAVLRNLPIISEVWKEISPIV
                     HRLMVDSGGFSVRGWSAQELLDELPELLNDTYAAGKQIKKIVEDLKDYARGSSGMPLD
                     AVDLSAVAAYAARLLGPLVERSTRRFQLDAPPGLPPTRADFQKLTQVAVNVLENALQS
                     LSGPDRGVRMTARSNPGQGTVLLECRDEGIGIDSSIRARIFEPFFTTKRDKGGTGLGL
                     SVSLGIVREYGGDIEIDSVAGEGTVVRVVLPVAGQERSA"
BASE COUNT          485 a          940 c         1027 g          572 t
ORIGIN      
        1 agcctgccca tcatcgagct cctggtgctc gagaagccgg tggacttctc gctccgctgg
       61 aggctctagt cggaaataaa gaaggccgcg atgaacgcgg cctcttcatg catcattaaa
      121 ttgtctttaa atcattttga attacgcacg catcttccga atggaacatc cgatttttac
      181 atgctctttt gcaatgattt tgcgcgagcg ccaatatgaa agcatagggc gtgccaaggc
      241 atgccacttt gcgcgattta tcgaagatga atattattcc ttccagagaa agagtttatt
      301 atattaatcg gtctggcgcc ggcccggatg gcggtatcgt gcttttttgc ccctgtgctc
      361 attttctcag gaatacccga gcgcaacgct ttgttttcaa tatgtcattc tatgatagag
      421 acttaaattc ctgtgccaat gcggtcttgc tgggcgatat ttcacacccc cgaatccgcg
      481 tcctcggcgc ccggttccgc gctccgcccc gccctcaggc gcttgttcag ggccgtccgg
      541 gacaggccta ccatgtcggc ggctatgccc tggttgccct tggcccgtcg gagtgcctcc
      601 ctcatgagct catcggtcgc gtccttgatg gtgggcagtt tcgcccatcc cgagaatctg
      661 gtcctgccgt cctcggcgac catcctggtc ccctcgccct tgccgccggc cctggtcttc
      721 tccctgaagc tgtcgagggc gagggtattg cccttgctgc ggctcaccgc gtcgtagacc
      781 atcgactcga gctcacggat gttcccggga aactcatagg actcgaggag gaccgagacc
      841 tcgtccggca cgatgggcat gctccttccg agccgctcgg ccgctgcctt cgcgaaatgg
      901 gagaggagga ggggcaggtc gtccagacgc tcgcgcaggg gcggcagctc gatcaggtgg
      961 gtctggaggc ggtagaagag gtcgagccgg aatgagcccg aggccgaggc cgcgcgcagg
     1021 tcgcggttgg tcgccgtcac gagggaggcc gtggtggcct tcggaaggtc ggccccgagg
     1081 gggtagtagc gcttgtcctc gatgagccgc agaagcttga cctgggagcc gggtgagagc
     1141 tcgccgatct cgtcgaggaa gagtgtcccc ccctcggccc tctctatgag gccgcccctg
     1201 tcggtctcgg ccccggtgaa ggccccgcgc ctgtggccaa agaggctgtc cgagaacatg
     1261 gtgtcgtcga ggccagcgat gttcaccctg acgagggagc cagcccggcc cgagaggagg
     1321 tggacggcct cggccacgag ctccttgccc gtccccgact cgcccaccac gaggatgggc
     1381 ttcggggact cggagaccgt ctccacgtag cgcaggacgc tgagcatgcg ttcgttgcgg
     1441 gtgatgatct tcgagaaggc cggggattgc gggggcctcg cggcgaggag ggtgtccttc
     1501 agggccgagt agtcgcgctg gagctcccgg atcgagagcg cgtgtctgat gctcgccgag
     1561 aggcggccgg attcggcggt cttggggaca tagtcgaagg cgccgaggcg catgcactcg
     1621 atggccgaat ccaggtcgct gacggccgtg gccacgatga cgggcacctc gggatggcgc
     1681 tcgcggacct cttgcaggac cctctgcccg gggatggcgg gcatgaggag gtccagcacc
     1741 atcacggaga tctcgcgctc acgcatgatg tcggggacca ggcgcggatc ggagcaggag
     1801 atgacctcgg ggaagccctc gaacttcagg agccgctcca tccgcttgag cacatcgggg
     1861 tcgtcgtcga cgacgaggat gggtttccgg gtcgcgtctg tcatgcgctg cgctcctgtc
     1921 cagcgacggg caggacgacg cgtaccaccg tgccttcccc cgccaccgag tcgatttcga
     1981 tgtctccgcc gtattcgcgc acgatgccca gggagaccga gagcccgagc cccgtccccc
     2041 ccttgtcgcg cttggtcgtg aagaagggct cgaagatcct cgccctgatc gaggagtcta
     2101 tcccgatgcc ctcatccctg cactcgagca ggacggtccc ctggccgggg ttggagcggg
     2161 ctgtcatgcg cacgccgcga tccggccctg acagggactg cagggcgttc tcgagcacgt
     2221 tgaccgcgac ctgggtgagc ttctggaagt cggcccgggt cgggggcagg ccgggcgggg
     2281 cgtcgagctg gaagcgcctg gtcgacctct ccacgagggg gccgagcagg cgggcggcgt
     2341 aggcagcgac cgccgagagg tcgacagcgt cgaggggcat gcccgaggag cccctcgcgt
     2401 agtccttcag gtcctccacg atcttcttga tctgctttcc ggcggcgtag gtgtcgttga
     2461 ggagctcggg cagctcatcc aggagctcct gggcgctcca gcccctgacg ctgaagcccc
     2521 cgctgtccac catgagcctg tgcacgatgg gcgagatctc cttccagacc tcggagatta
     2581 tcggcaggtt gcgcagcacc gcgttgttgg gcgtgttgat ctcgtgggcg accccggcgg
     2641 acaggacgcc gagggtcttc agcttctcga attccgctac gcggcgacgg gtctcgatcg
     2701 cctccgacag ggcccgcttc agggcgacgt gggtgtcgac gcgggcgaag aggagctcgg
     2761 tcggggccag tttgggcagg tagtcgctcg ccccggcccg gaaggcctcg gcgacggact
     2821 ccgagctgtc gcgatcggtc atcacgatga cggggagctc gctgagggac ttccttttgc
     2881 ggatccgttc gcagacgccg aggccgtcgc gcctcgggct cgaggcgtcg aggaggacga
     2941 ggtcgatgcg gggatcctcg acggacagac aggccgcccg ctccgaggag agctgggaca
     3001 gcacggcgta gccgcggtcc tcga
//
LOCUS       DASSQV010000308         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2311065, whole genome shotgun sequence.
ACCESSION   DASSQV010000308 DASSQV010000000
VERSION     DASSQV010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2311065"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /gene="pstC"
                     /locus_tag="VFL04_05350"
     CDS             <1..840
                     /gene="pstC"
                     /locus_tag="VFL04_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7839164.1"
                     /translation="ACAALSVLAVILITVFLFQRGLPLFRKVGILEFLGSTRWEPTAK
                     VPGYGILSFIVGSFYVTALALAFAVPIGLAVGIFLAELAQERSSRAIHSTVELLAGIP
                     SVIYGLFGIALVSTITRSLFGGSGYSVLSASIVLAIMTLPTIISVTEVSLRAVPRELK
                     EGSLALGATHWQTIVRVLVPAARSGIIAGVILGMGRAIGETMAVLMVGGNAPLMPKGL
                     TGMVRTLTMNIITDMSYATGDHLTSLFATGIVLFVFILVLNLTVQVALKRSIAATEGG
                     GGA"
     gene            833..2449
                     /gene="pstA"
                     /locus_tag="VFL04_05355"
     CDS             833..2449
                     /gene="pstA"
                     /locus_tag="VFL04_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET7839165.1"
                     /translation="MRNPRGGHAARLRSQGLAFGIIRFLSFLTIGVLALILGFIVFRG
                     LRYGNVVRSTVLSSSETAPDGLAIIVNDQSPLDRIEFPVLYGMYTDEYLNWSKITADK
                     EDLVPVALDPASPEGARVQAFLFGDAEPQWGGRVEKVADPDRAVERVRSTLGAVAVLP
                     ADSVAGQKGVKVVSLRRLVIAANSEVTALVGNSAIEDLSDSSLKRLMTGKVSNWKELG
                     GPDLAVRLLEPPAGSGLQKVIAEGAQAVSGGAGEKDMASYLAALKEQPGAVGYLFAGE
                     ALAAGIPALVWSGTESGWNLKLDYLLEAPGLSGKVGGISTIIANTFLMLVLTGLMAAP
                     IGVAAAVYLVEYAKQGIMIRILRLGTETLAGIPSIIFGLFGFLFFVNILGFGFGLLSG
                     CLTLTLMILPTIVRTSEEALKAVPAGLREGSLALGATKLQTIVRVAIPAAASGILTGL
                     ILGIGRAVGETAALLFTMGSDYKLAKGLFSSARVLSSHIYLLFAEGISFDRAFSTATV
                     LILVVVAFNFTAKRLIGSMGRGRGQGAKAR"
     gene            2446..>2939
                     /locus_tag="VFL04_05360"
     CDS             2446..>2939
                     /locus_tag="VFL04_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7839166.1"
                     /translation="MNGTAKFDIAGLDLWYGQTQALKQVSMQVAANSITALIGPSGCG
                     KSTFLRVLDRMNDLIGGVRTEGQVLYDGQDIFNGMDVIELRKRIGMVFQKPNPFPMSV
                     FDNVAYGPRVHGTRDRRELEEIVERSLVNAALFDEVKDRLHRSALGLSGGQQQRLCIA
                     RVLAV"
BASE COUNT          434 a         1115 c          895 g          495 t
ORIGIN      
        1 gcctgcgccg ccctctcggt cctggccgtc atcctgataa cggtcttcct cttccagcgc
       61 ggcctgcccc tcttccggaa ggtgggcatc ctcgagttcc tcggctcgac ccgctgggag
      121 cccactgcca aggtgccggg ctacgggatc ctctccttca tcgtgggctc cttctacgtc
      181 accgccctcg ccctcgcctt cgccgtcccc atcggcctgg ccgtgggcat cttcctcgcc
      241 gagctcgccc aggagcgctc ctcgcgggcc atccactcca cggtggagct cctcgcgggc
      301 atcccctcgg tcatctacgg cctcttcggc atagccctgg tctcgacgat cacgaggagc
      361 ctcttcggcg gctcgggcta ctcggtcctc tccgcctcga tcgtcctcgc catcatgaca
      421 ttgcccacga tcatcagcgt caccgaggtt tcgctgcgcg ccgtgccgcg cgagctcaag
      481 gaaggctccc tcgccctcgg ggcgacccac tggcagacca tcgtgcgcgt cctcgtcccc
      541 gcggcccgct cgggcatcat agcgggcgtc atcctgggca tgggccgcgc gataggcgag
      601 accatggccg tcctcatggt gggcgggaac gcccccctca tgcccaaggg cctcacgggc
      661 atggtcagga ccctcacgat gaacatcatc accgacatga gctacgcgac cggcgaccac
      721 ctcaccagcc tcttcgccac gggcatcgtc ctcttcgtct tcatcctggt gctcaacctc
      781 accgtgcagg tcgccctgaa gcggtcgatc gccgcgaccg aagggggcgg cggtgcgtaa
      841 tcccaggggc ggccacgctg ccaggctgcg aagccagggc ctggccttcg gcatcatccg
      901 cttcctctcc ttcctcacca tcggcgtcct cgccctgatc ctgggcttca tagtcttccg
      961 cggcctgcgc tacgggaacg tcgtgcgctc caccgtcctc tccagctccg agacggcccc
     1021 cgacggcctg gccatcatcg tgaacgacca gagccccctc gaccggatcg agttccccgt
     1081 cctctacggc atgtacaccg acgagtacct caactggtcc aagatcaccg ccgacaagga
     1141 ggacctcgtc ccggtcgccc tcgaccccgc aagccccgag ggcgcgaggg tccaggcctt
     1201 cctcttcggc gacgccgagc cccagtgggg cggccgcgtg gagaaggtgg ccgacccgga
     1261 ccgcgcggtc gagcgggtcc gcagcaccct cggcgccgtg gctgtcctcc cggcggactc
     1321 ggtggccggg cagaagggcg tgaaggtcgt ctccctgagg cggctcgtca tcgctgccaa
     1381 cagcgaggtc acggccctcg tcgggaacag cgcgatcgag gacctgagcg actcttcctt
     1441 gaagcggctc atgacgggca aggtgtccaa ctggaaggag ctcggcgggc ccgacctcgc
     1501 cgtccgcctc ctcgagcccc ccgcgggcag cgggctccag aaggtcatcg ccgagggcgc
     1561 tcaggcggtc tcgggaggcg cgggggagaa ggacatggcc tcctacctcg ccgcgctcaa
     1621 ggagcagccg ggcgccgtgg gctacctctt cgccggggag gccctcgcgg ccggcatccc
     1681 cgccctcgtg tggagcggca ccgagtccgg ctggaacctg aagcttgact acctcctcga
     1741 ggcccccggg ctctcgggca aggtgggcgg gatctcgacg atcatcgcca acaccttcct
     1801 catgctggtc ctcaccggcc tcatggccgc gcccatcggg gtggcggcgg cggtctacct
     1861 cgtcgagtac gcgaagcagg gcatcatgat caggatcctc cgcctcggca ccgagaccct
     1921 cgcgggcata ccctcgatca tcttcggcct cttcggcttc ctcttcttcg tcaacatcct
     1981 gggcttcggc ttcggcctcc tctcgggctg cctcaccctc accctcatga tcctgcccac
     2041 gatcgtgcgc acctcggagg aggccctcaa ggccgtccct gccggcctgc gcgagggctc
     2101 gctcgccctg ggcgccacca agctccagac catcgtgagg gtggccatac cggcggcggc
     2161 gagcggcatc ctcacgggcc tcatcctcgg catcggcagg gcggtgggcg agaccgccgc
     2221 actcctcttc acgatgggct cggactacaa gctcgcgaag ggactcttct cctcggcgcg
     2281 ggtcctctcc tcccacatct acctcctctt cgccgagggc atctcattcg accgggcctt
     2341 ctccacggcg accgtcctca tcctcgtggt ggtcgccttc aacttcaccg cgaagcggct
     2401 catcggcagc atgggccgcg gccgcggaca gggagcgaaa gcacgatgaa cggaacagcc
     2461 aagttcgaca ttgccgggct cgacctctgg tacggccaga cccaggccct gaagcaggtg
     2521 tcgatgcagg tcgccgccaa cagcatcacg gccctgatcg gcccctcggg ctgcggcaag
     2581 tccaccttcc tcagggtcct cgacaggatg aacgacctca tcgggggcgt gaggaccgag
     2641 ggccaggtcc tctacgacgg ccaggacatc ttcaatggca tggacgtcat cgagctgcgc
     2701 aagcggatcg gcatggtctt ccagaagccc aaccccttcc ccatgagcgt cttcgacaac
     2761 gtcgcctacg gcccgcgcgt ccacgggacc agggaccgcc gcgagctcga ggagatcgtg
     2821 gagcgcagcc tcgtaaacgc cgccctcttc gacgaggtca aggacaggct ccacaggtcg
     2881 gccctcggac tctccggagg ccagcagcag cggctctgca tcgcccgggt cctcgccgt
//
LOCUS       DASSQV010000309         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_694934, whole genome shotgun sequence.
ACCESSION   DASSQV010000309 DASSQV010000000
VERSION     DASSQV010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694934"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..802
                     /locus_tag="VFL04_05365"
     CDS             146..802
                     /locus_tag="VFL04_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HET7839167.1"
                     /translation="MIEEGDRILVAVSGGKDSTALAYDLSQKRKWWPVAFEVKAIHIA
                     TDFCSCCKKSALFDLLDSWGMPHVSLDVPVIGRLKPGKAMNCWWCSTQRRTELIRFAM
                     AEGYNKVALGHHLDDMVETLLMNMLYKSQISGMLPVVKYNKYPLSIIRPLALCEERQI
                     VAFADEKGFRSAVCTCPYGLESKRREVRKRIAALTGGSSELKRNLYDSMSNVNMDYLA
                     "
     gene            complement(799..1470)
                     /locus_tag="VFL04_05370"
     CDS             complement(799..1470)
                     /locus_tag="VFL04_05370"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET7839168.1"
                     /translation="MSAELWPQDRRFGSAALLAGGRSQRMGGVDKQEILVRGEGLGPR
                     AARLLSEAFGEVIVVTARTELYPGRGIRCVGDLIAGRGPLGGIHAALSASSSEWLYVM
                     ACDMPRFSAEYAAFLRAKLESILDGPGDPPLAAVTRFGAHIEPFHAYYSRALIPRLEG
                     LLGTMRPGGAEPSVRDLLAGLACVWVPEAEARRLSPDWSLFANINSPADLESLESVLA
                     GSGPD"
     gene            complement(1467..>2563)
                     /locus_tag="VFL04_05375"
     CDS             complement(1467..>2563)
                     /locus_tag="VFL04_05375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7839169.1"
                     /translation="RNLFSSSTSRILAEASISHYETVADELELRPTGYLWTWSERAWR
                     ASEPALRRLAKEAGRVEILDPLELRRVLAIGPEDPRFEAPAMGVWGRRCGSLSAMGLA
                     EHYARALTAAGGEIRTASLAGPPLADGSGSGGAPWASSRFTGLSDSRGETYSSGVLVA
                     AAGCWLQDLLGPAGLATGVYPKKRQLFGLRVGDWGSIFTRGSTGEAPNLILPAGQVYL
                     TPILSRGILVAGRADDLGRAFELPYEPASRAFAEEGFFRSAIEPVLRSYFPGLAKAYP
                     SGLEIASSWAGHYDYYWPDRNPVVERSADLVWVGGSSGSGIMKADSIGRVAAAKAVGD
                     EGVELADGTDFAVADLSLRKRRVAEEDLVI"
BASE COUNT          445 a          895 c          847 g          376 t
ORIGIN      
        1 cgagcggagg ctgtcccgtc ttcgcggccc gtggctcccc gctccccttg aaagcctgcc
       61 cctcaagccg ctaccatgtt ccaccgtgaa aaacgccgag acgacgctcg cgaagctcac
      121 cgaggtgggc atccgaagat attccatgat agaggaggga gaccgcatcc tggtcgcggt
      181 ctccggaggc aaggactcca cggcccttgc ctacgacctc tcccagaagc ggaaatggtg
      241 gccggtcgcg ttcgaggtaa aggccatcca catagccacc gatttctgct cctgctgcaa
      301 gaagtcggcc ctcttcgatc tcctcgactc ctggggcatg ccccacgtgt cgcttgacgt
      361 ccccgtcatc ggcaggctca aacccggaaa ggcgatgaac tgctggtggt gctccaccca
      421 gaggcggacc gagctcatcc gcttcgccat ggcggagggc tacaacaagg tggcgctcgg
      481 ccaccacctc gacgacatgg tggagaccct cctcatgaac atgctctaca agtcccagat
      541 ctcggggatg ctgcccgtcg tcaaatacaa taagtatccg ctctcgatca tacggcccct
      601 cgccctctgc gaggagaggc agatcgtcgc cttcgccgac gagaagggtt tccgctccgc
      661 ggtctgcacc tgcccctacg ggctcgagtc caagcgccgc gaggtccgta aacggatagc
      721 cgccctcacg gggggctcga gcgagctcaa gcggaacctc tacgactcca tgtcgaacgt
      781 gaacatggac tacctcgcct agtccgggcc tgagccagcc aggaccgact ccagggattc
      841 caggtccgcc ggactgttga tgttcgcgaa aaggctccag tccgggctga gacggcgggc
      901 ctcggcctcg gggacccaga cgcaggccag gccggcgagg aggtccctta ccgaaggctc
      961 agctcccccg gggcgcattg tgccgaggag gccctccagc ctcgggatca gggctcgcga
     1021 atagtaggcg tgaaagggct cgatgtgcgc gccgaaacgg gtcaccgcag caaggggcgg
     1081 atcccctggc ccatcgagga tcgactccag cttggccctc aggaaggccg cgtattcggc
     1141 tgagaagcga ggcatgtcgc atgccatgac gtagagccat tcgctggacg aggcggacag
     1201 ggcagcgtgg atccccccca ggggcccgcg gccggcgatg aggtccccga cgcagcggat
     1261 gccccttccc gggtagagct cggtcctggc cgtgacgacg atgacctcgc cgaaggcctc
     1321 cgacaaaagg cgggcggcgc gcggccccag gccttcgccc ctgaccagga tctcctgctt
     1381 gtccacgccc cccatcctct gactcctgcc tccggcgagg agggcggcgc tgccgaagcg
     1441 gcgatcctgg ggccacagtt ccgcgctcag atgaccaggt cctcttcggc gacccgtcgc
     1501 ttgcgcagcg agaggtcggc caccgcgaag tccgtcccgt cggcgagctc gacgccctcg
     1561 tcgccgacgg ccttggcggc tgccacccgg ccgatggaat cggccttcat gatgccggag
     1621 cccgaggagc cccctaccca gacgaggtcg gccgaccgct ctacgacggg gttcctgtcg
     1681 ggccaatagt agtcgtagtg tccggcccaa gagctggcga tctccaggcc cgaagggtag
     1741 gccttcgcca agcccgggaa atatgagcgg agcacgggct cgatcgccga gcggaagaaa
     1801 ccctcctccg cgaaggccct cgacgcgggc tcatacggga gctcgaaggc acggccgagg
     1861 tcatcggcgc ggccggccac gaggatgccc cgggagagaa tgggcgtcag gtagacctga
     1921 ccagccggca ggatgaggtt gggggcctct ccggtggagc cgcgggtgaa gatggacccc
     1981 cagtctccca ccctgaggcc aaagagctgt cgcttcttgg ggtagacccc cgtagcgagg
     2041 cccgcaggac cgaggaggtc ctggagccaa cagcccgcgg ccgcgaccag gacgccgctc
     2101 gagtaggtct cgccccgcga atcggagaga cccgtgaacc gggagctcgc ccatggggcc
     2161 ccgccggagc cggagccatc ggccagcggc ggcccggcga ggctggccgt cctgatctcg
     2221 ccgcccgccg ccgtcagggc tcgggcgtag tgctcggcaa ggcccatcgc ggacagggag
     2281 ccgcatcggc gcccccagac ccccatggcc ggagcctcga aacgcgggtc ctcgggcccg
     2341 atggccagga ccctcctgag ctccaggggg tcgaggatct cgaccctccc cgcctccttg
     2401 gccaggcgcc tcagcgccgg ctcggaggcc ctccatgccc gctccgacca ggtccagagg
     2461 tagcccgtcg gccgcagctc cagctcgtcg gccacggtct cgtaatggga gatcgaggcc
     2521 tcggccagga ttctgctggt cgaggaggag aagaggttgc ggt
//
LOCUS       DASSQV010000310         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1937513, whole genome shotgun sequence.
ACCESSION   DASSQV010000310 DASSQV010000000
VERSION     DASSQV010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937513"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VFL04_05380"
     CDS             <1..446
                     /locus_tag="VFL04_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839170.1"
                     /translation="LDGSLGGALRSRIAAAFLEAWRSPLRRALESSARFEAPARLAEL
                     ARGVDGIVQLGNCTGEGWFLTAEMVELIRAGADGIVCLQPFACLPNHVTGKGMLKELR
                     RRHPEVPIAPIDFDPGASEVNQLNRLKLLLESARRRRGAAGQERP"
     gene            complement(448..1515)
                     /locus_tag="VFL04_05385"
     CDS             complement(448..1515)
                     /locus_tag="VFL04_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HET7839171.1"
                     /translation="MRASTDPAPLHGHSLSPGPAAIALGLALIMGLCSCATDATLRAI
                     PVVGPGYRTPSDFRIVGYFPSWSGDPDLVQYRALTHINYAFLAPTMEGGYHPVDRPEK
                     LELLVTYAHAYGVRVLGSLGGWNDGGPNAYDTIAADPGLTAAFCDATMALVERYDLDG
                     IDMDWEFPKPATADNFARLMTALAERLHSCHKLLTIAVSATGYHGRDYSDELLSEVDF
                     LNIMAYDDGYKQPGVHHSSYAYAEAAMRYWIVERGLPITKAVLGLPFYGRSLVNGHAV
                     TYRSILSEDDDAAAKDVSGEYGYNGFDTIRSKTLDLAYANGGGVMIWQIAQDARGDRS
                     LLNAIFDAVKESWMPPGSRLQ"
     gene            1738..2301
                     /locus_tag="VFL04_05390"
     CDS             1738..2301
                     /locus_tag="VFL04_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7839172.1"
                     /translation="MADARTTTAAAAGREGKAPGARPIRIRVNGAWHEFATPSDLAPS
                     TTLAYLLRERLGLTGLKVSCDEGACGACTVLRDGKAVLSCMILAVEADGHEILTIEGL
                     PDDDPVIEAFAEQCEPGHGTALQCGYCSPGFVMTARALLNANPSPSLAEVKEALSGNL
                     CRCGCYQAIAQAVLRASAKIAERRRQS"
     gene            2298..>3486
                     /locus_tag="VFL04_05395"
     CDS             2298..>3486
                     /locus_tag="VFL04_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7839173.1"
                     /translation="MSRAFSPRVERRFIGKYRPKIDGKEKASGRAVYADDLSLKSRIP
                     GLLYAKVMRSPYAHARIKSLDIGAAKALAGVAQVMTYADHDLASLAPTNAGWTDGVDT
                     VSYRKMMWGTFRDRRVLGDYACWVGDEVGVAVAAETELIAEEALALVKVEWEVLPFVL
                     DPLEAMRPGAPLVHPEISADNVFPADPFGGEEVFVDKGRVEEGFAEAEVQVEGRSVYH
                     NATQSSLDNWCCVVDWKPDQLTVWSNSYAVDQTRMHVSQMLGLPLHRVRAISPYVGGQ
                     FGRGDTGDQPFFLFTALLSRRTGRPVKFRHTRRESFHDSRQPAIYFGKVGAMRDGRIT
                     AMHFRSIGNAGAYADHTMFALKYAPKEITEVALAHVPNVRFESRGVYTNKLPACMMRG
                     VGNS"
BASE COUNT          550 a         1207 c         1229 g          500 t
ORIGIN      
        1 ggctcgacgg gagcctgggc ggggccctcc gctcccggat cgccgccgcc ttcctggagg
       61 cctggcggag tccgctgcgg cgggccctcg agtcgagcgc ccgcttcgag gccccggccc
      121 ggctcgccga gctggcgcgg ggggtcgacg gcatcgttca gctgggcaac tgcacggggg
      181 agggctggtt cctgacggcg gagatggtcg agctgatcag ggcgggggcc gacgggatag
      241 tctgcctcca gcccttcgcc tgtctgccga accatgtgac gggcaagggc atgctcaagg
      301 agctcagacg gcgccacccc gaggtcccca tcgccccgat cgacttcgac cccggtgcct
      361 cggaggtcaa ccagctcaac aggctcaagc tcctcctgga gagcgcgcgc cgacggcgcg
      421 gcgcggccgg gcaggagagg ccctagctca ctggagcctg gagcccggcg gcatccagga
      481 ttccttcacg gcgtcgaaga tcgcgttcag gagcgaacgg tcgccccggg cgtcctgggc
      541 tatctgccat atcatgaccc cgccgccgtt ggcgtaggcg aggtcgaggg tcttggacct
      601 gatcgtgtca aagccgttgt agccgtactc ccccgagacg tccttggccg cggcatcatc
      661 gtcctccgag aggatgctcc ggtaggtgac ggcgtggccg ttcacgaggc tgcggccgta
      721 gaagggcagg cccagcaccg ccttggtgat cggcagcccc cgctcgacga tccagtagcg
      781 catggccgcc tcggcgtagg cgtaggagga gtggtggacg cccggctgtt tgtagccgtc
      841 gtcgtaggcc atgatgttga ggaagtccac ctcggagagg agctcgtcgc tgtagtcgcg
      901 gccgtggtag cccgtggcgc tcacggcgat ggtgaggagc ttgtggcagg agtgcagacg
      961 ctcggccagg gccgtcatga ggcgcgcgaa gttatcggcg gtggcgggct tggggaactc
     1021 ccagtccatg tcgatgccgt cgagatcgta gcgctccacg agggccatcg tggcgtcaca
     1081 gaaggccgcg gtgagcccgg ggtcggcggc gatggtgtca taggcgttgg gtccgccgtc
     1141 gttccagccg ccgagggagc cgaggaccct gaccccgtag gcgtgggcgt aggtgacgag
     1201 gagctcgagc ttctcgggcc tgtccacggg atgatagccc ccctccatcg tcggggcgag
     1261 gaaggcgtag ttgatgtggg taagcgcccg gtactggacg aggtcggggt cgcccgacca
     1321 cgaggggaag tagccgacga tgcggaaatc gctcggggtc ctgtagccgg gccccacgac
     1381 ggggatggcc ctgagcgtgg cgtcggtcgc gcaggagcaa aggcccatga tgagggcaag
     1441 gcccaaggcg atggccgccg ggcccgggct gaggctatgg ccatggagtg gtgctggatc
     1501 cgttgaagct cgcatgctgc aatcctgggt gccgacagaa tgtagcccgc cgcgcctcgg
     1561 ttttcaagcg cggccccggg gccgcgcgcc ggccctgccg cccgacctcg cctggcgccg
     1621 cccggcgccg ccgcctgacc gccctccccg ggggccaccc ccgaccaggg cccgttgagc
     1681 ttcttgcccg cggggcgggc cgagtccata ctgggccgaa agacgagggg ggaagccatg
     1741 gccgatgccc ggacgacgac cgcagccgca gcaggaaggg agggcaaggc gcccggggcg
     1801 aggccgatca ggatcagggt gaacggggcc tggcacgagt tcgccacgcc ctcggacctc
     1861 gccccctcca cgaccctggc ctacctcctc cgcgagcggc tcggcctcac cgggctcaag
     1921 gtctcctgcg acgagggcgc ctgcggagcc tgcaccgtcc tccgcgacgg gaaggccgtc
     1981 ctctcctgca tgatcctcgc cgtcgaggcc gacgggcacg agatcctcac catcgagggc
     2041 ctccccgacg acgaccccgt catcgaggcc ttcgccgagc agtgcgagcc gggccacggc
     2101 acggccctcc agtgcggcta ctgcagcccc ggcttcgtga tgacggcgag ggccctcctc
     2161 aacgccaacc cgagccccag cctggcagag gtcaaggagg ccctctcggg caacctctgc
     2221 cgctgcggtt gctaccaggc gatagcccag gccgtgctcc gcgcctcggc caagatcgcg
     2281 gagcggagga ggcagtcatg agcagggcct tcagccccag ggtcgagcgc aggttcatcg
     2341 gcaagtacag gcccaagatc gacggaaagg agaaggcgag cggcagggcc gtctacgccg
     2401 acgacctctc gctcaagagc aggatccccg gcctcctcta cgcgaaggtc atgcggagcc
     2461 cctatgccca cgcgcgcatc aagagcctcg acatcggcgc cgcgaaggcc ctggcgggcg
     2521 tggcccaggt catgacctac gccgaccacg acctcgcctc gctcgcgccc acgaacgccg
     2581 gctggaccga cggggtggac acggtctcct accgcaagat gatgtggggg accttccgcg
     2641 accgccgcgt cctcggcgac tacgcctgct gggtgggcga cgaggtcggc gtggccgtcg
     2701 cggccgagac cgagctcatc gccgaggagg ccctcgccct ggtcaaggtc gagtgggagg
     2761 tcctgccctt cgtcctcgat cccctcgagg cgatgaggcc cggggccccc ctcgtccacc
     2821 ccgagatctc agcggacaac gtcttcccgg ccgacccctt cggcggcgag gaggtcttcg
     2881 tggacaaggg tcgggtcgag gagggcttcg ccgaggccga ggtccaggtc gagggccgct
     2941 cggtctacca caacgcgacc cagagctcgc tcgacaactg gtgctgcgtc gtcgactgga
     3001 aacccgacca gctcaccgtc tggtccaaca gctacgccgt cgaccagacg cggatgcacg
     3061 tgagccagat gctcggcctg cccctccacc gggtccgcgc gatcagcccc tacgtcggcg
     3121 gccagttcgg ccgaggcgac acgggggacc agcccttctt cctcttcacc gccctcctgt
     3181 ccaggcgcac ggggaggccg gtcaagttca ggcacacgag acgggagtcc ttccacgact
     3241 cccgccagcc cgcgatctac ttcggcaagg tgggcgcgat gcgcgacggc aggatcaccg
     3301 cgatgcactt caggtccatc gggaacgcgg gggcctacgc ggaccacacg atgttcgcac
     3361 tcaaatacgc ccccaaggag atcaccgagg tggccctcgc ccacgtgccc aacgtgaggt
     3421 tcgagtcgag gggagtctac accaacaagc tccccgcctg catgatgcgc ggagtgggca
     3481 actccc
//
LOCUS       DASSQV010000311         5427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2187966, whole genome shotgun sequence.
ACCESSION   DASSQV010000311 DASSQV010000000
VERSION     DASSQV010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187966"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFL04_05400"
     CDS             <1..894
                     /locus_tag="VFL04_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET7839174.1"
                     /translation="RPMRAFSLVFYPLSKFLSATTELIVGAIIAKGKQEPAVTEEEVR
                     VLIAQGTESGVFEDREREMVEGVLSLGDRRVTSLMTPRTELVAIDLSQGAEQARRIVL
                     TNARYGCLPAVEESLDHVVGMISVKESLAAIAEGRFDEPRRFVRTPVLIPETMTALKA
                     FSALTGGEVKSALILDEYGGVSGLVSLSDLMEAIVGDIPLTGYEEEPEIVRREDGSYL
                     VDGSLPIERFIDELSLDESLVEGDYDTVAGLVLDRMGTIPKAGEKCRWDGCVLEIVDM
                     DGNRIDKILVTLDRAKEDPEE"
     gene            complement(931..1494)
                     /gene="pdxT"
                     /locus_tag="VFL04_05405"
     CDS             complement(931..1494)
                     /gene="pdxT"
                     /locus_tag="VFL04_05405"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET7839175.1"
                     /translation="MKKVGVLALQGDFDAHRVAIERAGASAFDVRTLADIESADALVL
                     PGGESTVMGSLLVRFGLMDALVKRIKAGLPVFGTCAGLILLAKTVEDRDQPGIRLLDV
                     AVKRNAYGRQVDSFRAPITTRIPGAESLEAVFIRAPRISALGKGVEVLAEYKADPILV
                     RQGNILGGSFHPELVDGAAIHAYFLAM"
     gene            complement(1497..2402)
                     /gene="pdxS"
                     /locus_tag="VFL04_05410"
     CDS             complement(1497..2402)
                     /gene="pdxS"
                     /locus_tag="VFL04_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET7839176.1"
                     /translation="MDKGLWSERQSSEWKTKVGLAVMLKGGVIMDVVNAEQAKIAEKA
                     GAAAVMALERVPADIRAHGGVARMSDPKMIKEIQDAVSIPVMAKCRIGPFVEARILEA
                     LEVDFIDESEVLTPADDRYHVMKHDFVVPFVCGCRDLGEALRRIGEGAAMIRTKGEPG
                     TGDIVEAVRHIRCVNDEIARLSRLRDEELMTAAKELGAPFELVVEVAKTGKLPVPNFS
                     AGGVATPADAALMMQLGAEAVFVGSGIFKSGDPTRRATAIVGAVTYYKDPKMLAKLSE
                     DLGEPMVGIGLSELPEGERMAKRGW"
     gene            2850..3041
                     /locus_tag="VFL04_05415"
     CDS             2850..3041
                     /locus_tag="VFL04_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839177.1"
                     /translation="MTTQVSTIELPSTGIVYGFPTAERARTIGARSLPDCELTLRQSR
                     WSSPHPNGEDRQVKMIRVG"
     gene            3142..4746
                     /locus_tag="VFL04_05420"
     CDS             3142..4746
                     /locus_tag="VFL04_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839178.1"
                     /translation="MKTLICGIALLSLVACFPLAAQDLGGPWTIRKVDFQVTGRSLAF
                     VMMKKIDPLGTMVGSSFPDRAALESFIADRRQVLMNERVLASVETGYDSKPSSSGGYD
                     VDLRFTTVDTWNLIALPYAKYDSNTGLLLSLRGRDYDFAGSMQELALDLNYQKDPAGR
                     VSYTGALSFSLPFQALGRDWAFGLQEAGGLWTDGTASTVTQASLSLDLPGLGFPADIT
                     LTQGFSYNALAGAPLPANDPDSWYLSEGLALRASIPLTGSMGSLDSVELGPLNYSPGI
                     AVSLNWNPATRLSYYGSGTVTEAGNGPYGVGDTVPAIGNGISYGRGGLVTTASNSLSL
                     GRVDWRGNQRAGLSLMIANNTSYNAQYRDLASDLNLTAAWYGESRGRIGVAAMLTILN
                     RFSNHPKADALANLGWYLRGVLDDRISGTKAGFVNLELPIKLFDFPTHSVIGTRTLDF
                     ELQIAPFADAAIVPSGYGSQPNADQFWYTGGREFLAFPAGLRSFIVRASAGWDLKSVI
                     ATRSLSAKTTDGSSPYEIFAGLGLKY"
     gene            4908..>5427
                     /locus_tag="VFL04_05425"
     CDS             4908..>5427
                     /locus_tag="VFL04_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7839179.1"
                     /translation="MKKAASPPEDRFALLAANSPDTIYLLDLPSHRLLFLNRDEFLGY
                     SRDELESGDSILSNLHPDDRERVRSQWIDLVDGRAGQTGDLDYRLRRKDGRWSWLRCR
                     QSIIARDGKGKPVQLLVTLTDIDAQARRERRSAHQNRVLKAMASGLSLPAVLELIGRG
                     VEEEEASGHCVIL"
BASE COUNT          941 a         1833 c         1717 g          936 t
ORIGIN      
        1 cggccgatgc gcgccttcag ccttgtcttc tatccactgt ccaagttcct ctcggccacg
       61 accgagctca tagtgggcgc cataatcgcc aagggcaagc aggagcccgc cgtcaccgag
      121 gaggaggtcc gcgtcctcat cgcccagggc acggagtcgg gggtgttcga ggacagggag
      181 cgcgaaatgg tcgagggcgt cctctccctc ggtgaccgcc gcgtgacctc cctcatgacg
      241 ccgaggacgg agctggtggc gatcgacctg tcgcagggcg ccgaacaggc ccgccgaatc
      301 gttctcacga acgcccgcta cggctgcctc ccggccgtcg aggaaagcct cgaccacgtc
      361 gtgggcatga tctccgtgaa ggagtccctg gccgcgatcg ccgagggtcg tttcgatgag
      421 cccaggcggt tcgtccgaac gcccgtcctc atcccggaga cgatgacagc cctcaaggcc
      481 ttctcggcgc tcaccggcgg cgaggtgaag agcgccctca tcctcgacga gtacggcggc
      541 gtgtccggcc tcgtgtccct ctccgacctc atggaggcca tcgtggggga cataccgctc
      601 acgggctacg aggaagagcc cgagatagtg cgccgcgaag acggcagcta tctcgtggac
      661 ggctccctcc ccatcgagcg cttcatcgat gagctgtccc tcgacgagag cctggtggag
      721 ggggactacg acaccgtcgc tggcctggtc ctcgaccgca tggggacgat ccccaaggcg
      781 ggcgagaaat gccgctggga cggctgcgtg ctggagatcg tggacatgga cggcaacagg
      841 atcgacaaga tcctcgtgac cctcgacagg gccaaggagg atcctgagga ataggatggc
      901 ggtccggggg gaaggtcccc ccggacccgt ctacattgcc agaaaatagg cgtggatggc
      961 ggcgccgtcc acgagctcgg gatggaagct tcccccgagg atgttgccct ggcggaccag
     1021 gatggggtca gccttgtact cggcgagcac ttcgacgccc tttcccaggg cgctgatcct
     1081 cggggccctg atgaagacgg cctcgaggct ctcggccccg ggtatcctcg tcgtgatcgg
     1141 cgcccggaag ctgtccacct ggcggccata ggcgttccgt ttcaccgcca cgtcgagaag
     1201 cctgatgccc ggctggtccc gatcctcgac cgtcttcgcg aggaggatca ggccggcgca
     1261 ggtgccgaac acggggagcc cagccttgat ccgcttcacg agggcatcca tgagcccgaa
     1321 acggacgagg aggctcccca tgacggtgct ctcgccgccc ggcaggacga gggcgtcggc
     1381 cgactcgatg tcggcgagcg tccggacatc gaatgccgag gcgcccgccc gctcgatcgc
     1441 gacgcggtgg gcgtcgaagt cgccctggag ggcgagaacg ccgaccttct tcatcctcac
     1501 cagccccgct tcgccatgcg ctcgccctcg gggagctcgc tgaggcctat gccgaccatg
     1561 ggctcgccca agtcctcgga gagcttcgcg agcatcttcg ggtccttgta gtaggtgacg
     1621 gcgccgacga tcgcggtcgc ccgccgcgtc ggatcccccg acttgaagat gccggaacct
     1681 acgaagacgg cctccgcgcc gagctgcatc atgagggcgg cgtcggccgg cgtcgccacc
     1741 ccgcctgcgg agaagttcgg gacgggcagc ttgcccgtct tcgcgacctc cacgacgagc
     1801 tcgaaaggag cgccgagctc cttggcagcg gtcatgagct cctcgtcgcg cagacggctc
     1861 agccgagcga tctcgtcgtt gacgcacctg atgtggcgga ccgcctcgac gatgtcgccg
     1921 gtgccgggct cgcccttggt ccggatcatc gccgcgccct cgccgatgcg gcggagggcc
     1981 tcgccgaggt cgcggcagcc gcagacgaag ggcacgacga agtcgtgctt catgacgtgg
     2041 tagcggtcgt cggccggggt gaggacctcg gattcgtcga tgaagtcgac ctcgagggcc
     2101 tcgaggatgc gggcctcgac aaaagggccg atccggcact tcgccatcac agggatgctc
     2161 acggcatcct ggatctcctt gatcatcttc gggtcggaca tgcgcgccac gccgccgtgg
     2221 gccctgatgt cggcggggac gcgctccagg gccatgaccg ccgccgctcc cgccttctcg
     2281 gcgatcttcg cctgttcggc gttgacgacg tccatgatga cgccgccctt gagcatcact
     2341 gcaagtccga ccttggtctt ccactcggac gactggcgtt cggaccacaa gcccttatcc
     2401 atgcgcttcc ctcctgcctt taccgggagc cgtctgcggc ttccaaggcc aaaatacaga
     2461 atatggcgaa tctgttcaag aatgcgggga gcctagcacg aagggcccaa attgaggaag
     2521 ccgacgctgg ccgagcgccg cctgcagtcg tcgtccagag gccgggaggg gggacggggc
     2581 gcgggggccg gcctggggcg aggcggccct ggacccctgg gtcgaaaaac cccgggcatg
     2641 caaggaggcg ctcatgcccg ggggaggaaa cctaagcaat ttaatattaa cataaccgga
     2701 ttcctgacgc aaggcccgga cccgaggaag cggcgttccc gggaggcacc agctgccgcc
     2761 gggggcccca agccccaatt tggcgatatt tccccccttg ctctttacag accctccctc
     2821 atagttatat tttattaccg gaggcaagca tgacgacaca ggtttccacg atagagctac
     2881 cgtcgaccgg gatcgtctac gggttcccca ccgccgagag ggcacgcacc atcggcgcgc
     2941 gctccttgcc cgactgcgag ctgacgctgc ggcaatcccg gtggtcatca ccccacccca
     3001 atggcgagga caggcaggtc aagatgatca gggtcggctg aggcgattcg ctgcggcggg
     3061 gctcccgcct cggcgatcgc ccgtatcggg catccttgat gccttgcgca gtatctacca
     3121 tccatggcaa gaacactcat cgtgaaaacc ctcatatgcg gcatcgcgct tctctccctt
     3181 gtggcctgct ttcccttggc cgcccaggac ctcggcggcc cctggaccat caggaaggtc
     3241 gatttccagg tgaccggacg cagcctcgcc ttcgtgatga tgaagaagat cgaccccctc
     3301 ggcaccatgg tcgggtccag cttccccgac agggccgcgc tcgaatcctt catcgccgac
     3361 cgccgacagg tactcatgaa cgagagggtc cttgcctcgg tcgagacggg ctatgattcg
     3421 aagccctcct cgtcgggcgg atacgatgtc gatctcaggt tcaccaccgt cgacacgtgg
     3481 aacctcatag ccctgcccta cgcgaagtac gactcgaaca ccggccttct cttgagcctc
     3541 cgcggccggg actacgactt cgccggctcg atgcaggagc ttgccctcga cctgaattat
     3601 cagaaggatc cggccgggcg ggtctcctat acgggtgccc tgagtttctc actgcccttc
     3661 caggccctgg gccgggattg ggccttcggc ctccaggagg ccggagggct gtggaccgac
     3721 ggcacggcga gcaccgtaac ccaggcgagc ctctccctcg accttcccgg cctcggcttc
     3781 ccggcagaca taaccctgac ccaaggcttc tcctacaacg cccttgcggg agcccccctc
     3841 ccggcgaacg acccggacag ttggtacctc tcagagggcc ttgctctccg ggcgagcatt
     3901 cctttgaccg gctccatggg ctcattggac tcggtcgagc ttgggccgct caattacagc
     3961 ccgggcatcg cggtctcgct caactggaac ccggccacaa ggctctcata ctacgggagc
     4021 ggcaccgtca ccgaggcagg caacggtcca tacggggtcg gcgacaccgt gcctgcgatc
     4081 gggaacggga tctcctacgg gcgtggcgga ctcgtcacca cggcctcgaa ctccctcagc
     4141 ctcggccgag tcgactggag ggggaaccag cgtgcggggc tgagcctcat gatcgcaaac
     4201 aatacctcct acaatgccca atacagggac ctcgcctcgg acctgaacct cacggctgcc
     4261 tggtatggcg aatcgcgggg ccggatcggg gtcgccgcca tgctcacgat cctgaaccgt
     4321 ttttcgaacc accccaaggc cgacgccctc gccaatcttg gctggtatct gcgtggagtc
     4381 ctcgacgaca ggatctcggg gaccaaggcc ggcttcgtga accttgagct gcccatcaag
     4441 ctcttcgatt tcccgaccca ttccgtgatc gggacgagaa ccctggattt cgagctccag
     4501 atcgccccct tcgccgatgc agccatcgtg ccctccggtt acgggtcaca gcccaacgct
     4561 gaccagttct ggtacacggg cggccgagag ttcctggcct tccccgccgg tcttcgcagt
     4621 ttcatcgtca gggccagcgc gggctgggac ctcaagagcg tcatcgcgac tcgatccctg
     4681 tccgcaaaga ccacggacgg cagcagcccc tacgagatct tcgcgggact cggcctcaag
     4741 tactaggcgg gtatgggtcg agttgcgcgc cggaggttac atttctattc cagcgggggg
     4801 ttttcgcttt ccgctcccga tgggatttca gtatctatga ggtgttgagg gtcaggggtc
     4861 tccggttcgg gcagggcccc attcgggatg gccaggggag gcgaggattg aagaaagctg
     4921 cgagtcctcc cgaagacagg ttcgccctcc tcgccgccaa ctcccccgac acgatctacc
     4981 tactcgatct cccctcccac aggctgctct tcctcaacag ggacgagttc ctcggctaca
     5041 gccgcgatga gctcgaatcc ggcgactcga tactctccaa cctccatccc gacgacaggg
     5101 aaagggtcag gtcgcaatgg atcgacctgg tcgacggccg ggcgggacag accggggacc
     5161 tggattaccg gctgaggcgc aaggatggcc gctggtcctg gctccgctgc cgacaatcga
     5221 tcatcgcgcg agacgggaag ggcaagcctg tccagctcct cgtcaccctg acggacatcg
     5281 atgcgcaggc gaggagggag cgcaggtccg cgcatcagaa ccgggtgctc aaggccatgg
     5341 cctccggtct ttccctcccc gccgtgctcg aactcatcgg tcggggagtc gaggaggagg
     5401 aggcgagcgg ccactgcgtg atccttc
//
LOCUS       DASSQV010000312         3616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1689507, whole genome shotgun sequence.
ACCESSION   DASSQV010000312 DASSQV010000000
VERSION     DASSQV010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689507"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFL04_05430"
     CDS             <1..324
                     /locus_tag="VFL04_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dehydrogenase"
                     /protein_id="HET7839180.1"
                     /translation="ELLELIVVPADTAGARGSYRKLKRINGHDLGIVGVAVMKKAGLM
                     RIAVSSAAPTPVLVDGLAEDTSPDAAVAAVRKAISPISDLRCSKEYREHMVEVFVRRL
                     LEELR"
     gene            321..809
                     /locus_tag="VFL04_05435"
     CDS             321..809
                     /locus_tag="VFL04_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7839181.1"
                     /translation="MIRVNVNVNGRQYQREVEPTKTLLRFLREDLGLTGTKEGCGAGE
                     CGACTVFLDGHTVNSCMVLAAELDGRSVETIEGEAKEGILSDIQAAFDRHHAIQCGYC
                     SAGMVLSVRELLKRNPKPTVDQIKEGIEGNFCRCTGYQQIIEAVLDVSGQLERKGELH
                     YV"
     gene            802..3255
                     /locus_tag="VFL04_05440"
     CDS             802..3255
                     /locus_tag="VFL04_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7839182.1"
                     /translation="MFEQEKPRSASHVGKAERRIEAVAKVTGDAVYTSDMAMPGMLHA
                     QVKKSPHARARIVSIDTSKAEALAGVRAIITGRELNYRLGLYVVDKDILAKDEVRHYG
                     EAVAAVAADTLEIAAEAVGLIEVKYEVLSPVLNHMDALKPGAPLVHPDLGKYDYVPVF
                     SPQAGTNIPNLSKLRKGDVEKGFEDSEWIVEREYTNPSVQHVPMETHVAIVEWKNGDE
                     VTIWTSAQSPFTVRNLFCYTFKLPLNKVRVVVPHVGGGFGGKAGIHLEPMLACLSRKA
                     GGKPVKLMASREEEFSLLPCRSALTYRIKTGIAADGKILAQKMTLYWDAGAYADYAVN
                     VTRASGYSAAGPYEIPNAWIDAYTIYTNKPYGTAYRGFGHVEFFWGLERHMDLIASVI
                     GMDRLEFRKLNLLKPGSITITGERISEHTGDPTKCLEAVAKAIGFGKLTPAEEARQKR
                     TGRRIGKGVATLHKAPAMPSFTATAAVVKMNSDGSVIVNVALTEIGQGSATAIAQIAA
                     ERLRFPLSRVKVTIEKDTDKEPYDWQTVASKGLILSGNATGLACDDMLRQAYEVAAQV
                     LRANVADLDHDADRVFVRHSPERGVGFAALSVGYAVPDGNALGGPLIGVGRDTAQGRT
                     NLDKATGQGLPALDWTFGAHGVIAEVDPDTGEYKIIKIASAFDVGRVINPDILRGQCI
                     GGMIQGLGTAICEGYIYDEAGHLLNPSFTDNKIPTAKDLPEEIESIVVETAQVDGPYG
                     ARGVGEHPMISVAPALGNAINEAVGAELLHMPIRFEDVWRALNTKEAIDNWITKTPSG
                     SCRSDPATRVNSPDSAAAP"
BASE COUNT          676 a         1267 c         1175 g          498 t
ORIGIN      
        1 gagctcctcg agctcatcgt cgtgccggcc gacacggcgg gggccagggg ctcctaccgc
       61 aagctgaaac ggatcaacgg ccacgacctc ggcatcgtcg gggtggcggt gatgaagaag
      121 gccggcctca tgcggatcgc cgtctcctcg gccgcgccga cccccgtcct cgtcgacggc
      181 ctcgccgagg acacgagccc tgatgccgcg gtggccgccg tgcgcaaggc gatcagcccg
      241 atcagcgacc ttcgctgcag caaggaatac agggagcaca tggtcgaggt cttcgtgcga
      301 aggcttctgg aggagctgcg atgataaggg tgaacgtgaa cgtcaatggc aggcagtacc
      361 agagggaggt cgagcccacg aagaccctcc tgcgcttcct ccgcgaggac ctcggcctca
      421 ccgggaccaa ggagggctgc ggggccggcg agtgcggagc ctgcaccgtc ttcctggacg
      481 gccacaccgt caactcctgc atggtgctcg cggccgagct cgacggccga agcgtcgaga
      541 cgatcgaggg cgaggcgaag gagggcatcc tgagcgacat ccaggctgcc ttcgaccgcc
      601 accacgcgat ccagtgcggc tactgcagcg cgggcatggt cctctcggtc cgcgagctgc
      661 tcaagcggaa cccgaagccc acggtagacc agatcaagga ggggatcgag ggcaacttct
      721 gccgctgcac aggctaccag cagatcatcg aggccgtgct cgacgtctcg ggccagctcg
      781 agcgcaaggg ggagctgcac tatgtttgag caagagaaac cgcggagcgc ctcccacgtc
      841 gggaaggccg agcgccggat cgaggccgtg gccaaggtca ccggcgacgc cgtctacacg
      901 agcgacatgg ccatgccggg catgctccac gcccaggtca agaagagccc ccacgcgagg
      961 gcccgcatcg tgagcatcga cacctcgaag gccgaggccc tggccggggt ccgcgcgatc
     1021 atcaccggca gggagctcaa ctaccgcctc ggcctctacg tggtggacaa ggacatcctc
     1081 gcgaaggacg aggtgaggca ctacggcgag gccgtcgccg ccgtcgccgc cgataccctc
     1141 gagatagccg ccgaggcggt gggcctcatc gaggtcaagt acgaggtcct gagccccgtg
     1201 ctcaaccaca tggacgccct gaagcccggg gcccccctcg tccaccccga cctcggcaag
     1261 tatgactacg tgccggtctt cagcccccag gcggggacca acatcccgaa cctgtccaag
     1321 ctcaggaagg gcgacgtcga gaagggcttc gaggactccg agtggatcgt cgagcgcgag
     1381 tacaccaacc cctcggtcca gcacgtgccc atggagaccc atgtcgccat cgtcgagtgg
     1441 aagaacggcg acgaggtcac catctggacg agcgcccagt cgcccttcac ggtgcgcaac
     1501 ctcttctgct acaccttcaa gctgcccctc aacaaggtgc gcgtcgtggt gccccacgtg
     1561 ggcggaggct tcggcggcaa ggccggcatc cacctcgagc ccatgctcgc ctgcctctcc
     1621 cgcaaggccg gcggcaagcc cgtgaagctc atggcgagcc gcgaggagga gttcagcctc
     1681 ctgccctgca ggagcgccct cacctacagg atcaagacgg gcatcgccgc cgacgggaag
     1741 atcctcgccc agaagatgac cctgtactgg gacgcgggcg cctacgccga ctacgccgtc
     1801 aacgtgacca gggcctcggg ctactcggcg gcggggccct acgagatccc caacgcctgg
     1861 atcgacgcct acacgatcta cacgaacaag ccctacggca cggcctaccg cggctttggc
     1921 catgtcgagt tcttctgggg cctcgagcgc cacatggacc tcatcgcctc ggtgatcggc
     1981 atggaccgcc tcgagttccg gaagctgaac ctcctcaagc ccggctcgat cacgatcacc
     2041 ggcgagagga tcagcgagca caccggcgat cccaccaaat gcctcgaggc agtcgccaag
     2101 gccatcggtt tcgggaagct cacgcctgca gaggaagcca ggcagaagcg gacggggcgg
     2161 aggatcggca agggggtcgc gaccctgcac aaggcccccg ccatgccctc cttcaccgcc
     2221 acggccgccg tcgtgaagat gaactccgac ggctcggtga tcgtcaacgt cgccctcacc
     2281 gagatcggcc agggctcggc cacggccatc gcccagatcg ccgccgagcg gctccgcttc
     2341 cccctctcaa gggtcaaggt caccatcgag aaggacaccg acaaggagcc ctacgactgg
     2401 cagaccgtgg cctccaaggg cctgatcctt tcgggcaacg cgacgggcct ggcctgcgac
     2461 gacatgctgc ggcaggccta cgaggtcgcg gcccaggtcc tgcgcgccaa cgtcgccgac
     2521 ctcgaccacg acgccgacag ggtcttcgtg aggcactccc ccgagcgcgg cgtcggcttc
     2581 gccgccctct cggtgggcta cgccgtcccc gacggcaacg ccctcggcgg gcccctcatc
     2641 ggggtgggcc gcgacacggc ccagggccgc accaacctcg acaaggccac gggccagggc
     2701 ctgcccgccc tcgactggac cttcggcgcc catggcgtca tcgccgaggt ggaccccgac
     2761 accggcgagt acaagatcat caagatcgcc tcggccttcg acgtcggcag ggtcatcaac
     2821 cccgacatcc tccgcggcca gtgcatcggc ggcatgatcc agggcctcgg cacggccatc
     2881 tgcgagggct acatctacga cgaggcgggc cacctcctca acccgtcgtt cacggacaac
     2941 aagatcccga ccgcaaagga cctgcccgag gagatcgaga gcatcgtcgt ggagacggcc
     3001 caggtcgacg ggccctatgg ggcccggggc gtgggcgagc acccgatgat ctcggtggct
     3061 cccgccctcg gcaacgccat caatgaggct gtcggggccg agctcctcca catgcccatc
     3121 cgcttcgagg acgtctggag ggccctgaac accaaggagg ccatcgacaa ctggatcacc
     3181 aaaaccccct cgggctcctg cagatcggac ccggccaccc gggtcaacag ccccgactcg
     3241 gccgccgcgc cgtaaggcga agaaaaggga attcagaggc aaccacggag gcacggagaa
     3301 agcggagaag aagaaaaggg ggcaggggcc agcgcgtggc tcttgcccct attcattggc
     3361 tggggtcccg ttctcgccgg ctccgtgagg gccgatccta gaggtcttgc tcttcttacc
     3421 ctctttccct ccgcgcactc cgcgctctcc gcggtgagaa tgtcgctagc ccttcagcgc
     3481 agcccggtac tcctcctcgg tgtcgaggtc gcgcaggatg gactcgtcgg gaagctcgag
     3541 gtaggagggc gagctcgcca tgaggagggg ccggaggggc tcggtgggcg ggatctcgag
     3601 gatcgagggg atcagc
//
LOCUS       DASSQV010000313         3345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1816378, whole genome shotgun sequence.
ACCESSION   DASSQV010000313 DASSQV010000000
VERSION     DASSQV010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816378"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="VFL04_05445"
     CDS             complement(<1..1473)
                     /locus_tag="VFL04_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7839183.1"
                     /translation="MSDTVRREFDDGGPYFSAKAKYPGILGWLTATNHKRIGILYLCL
                     IGTFFTAAVILGVLMRIALFNPGSTFVSPATYNALFTLHGIIMVFLVVIPGVPTIFGN
                     FCLPMLIGANDMAFPRLNLFTWYLFLAGALLAFTSVFVNGGAPDTGWTFYVPFSLKTT
                     VDVPLATFAVFVLGMSSILTGINIVTTVHQLRAPGMRFFNMPLSVWGLYSTAWVQILA
                     TPIIGITMLLIVVERLFGIGVFDPTKGGDPLLYQHLFWIYSHPAVYIMILPAMGVMSD
                     LFPVFSHKNVFGYKMIAFSSLAIASVGSLVWGHHMYVSGQSDFAGIVFSLLTFLVAVP
                     SAIKVFNWISTMYKGSIRLEPPFLFALSFVFLFSIGGLTGLFIGALSVDVQLHDTYFI
                     VGHFHYVIFGGMGYGTMAALHYWFPKITGRMVAKRPAIIGWLVSFVGFNLFYFPMLIM
                     GWEGMPRRYADYLPQFTDIQRFSSMGAWIMVAGLVIIFGNL"
     gene            complement(1470..2291)
                     /locus_tag="VFL04_05450"
     CDS             complement(1470..2291)
                     /locus_tag="VFL04_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET7839184.1"
                     /translation="MSRGPRALPLALLLGFMAGAAAVAHTVPSQAAAAALASPLGFDE
                     RLGSRIALDASFTGEDGKAVSMAELVKTPVVLNFVYYRCKDQCNVLLTGIGLALRGLQ
                     GVPGVDYSVVTVSVNDREGPKDALEKKAIALASIERPFPPSAWRFLVGDDASIDALAD
                     SVGLSYVRHGDDFDHPLGLVIIAPGGKIVRYMNGSDFLPLDLSMSVLEASKGIVKPTV
                     AKMLRFCFNYNPGSRQMRFDLMRVSGIVIGLLVASFAAYLVVSGRKQRSYRKGVR"
     gene            complement(2298..3074)
                     /locus_tag="VFL04_05455"
     CDS             complement(2298..3074)
                     /locus_tag="VFL04_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7839185.1"
                     /translation="MLDAMYALLARMGFTDPLHAPITHMPIGLITGSLVFFVTAIIFK
                     RRQLITSARHASILALLFVFPTILFGVFDWIHFYHAVLFPAIKIKMVLAGTVLLLLGA
                     GIILGSEVKIRAFWMTAIYALAFVAVVGLGWFGAGIIYGRGIAVTAETKAVVAAAPGA
                     PAEATAPGAPSSGGDLNAGQATFNANCQGCHPSGGNIIVPLLPIKGSKQLKTLEAFTA
                     FIRNPTMPNGGAGEMPPFGTDQVSDAKAKDLYAYVTTTWK"
BASE COUNT          633 a          939 c         1256 g          517 t
ORIGIN      
        1 gaggtttccg aagatgatca cgagaccggc caccatgatc caggccccca tgctcgagaa
       61 gcgctggatg tcggtgaact ggggcaggta gtcggcgtag cgccggggca tgccctccca
      121 gcccatgatg agcattggga agtagaagag gttgaagccg acgaagctga cgagccagcc
      181 gatgatggcc gggcgtttgg ccaccatgcg gccggtgatc ttggggaacc agtagtggag
      241 agcggccatc gtgccgtagc ccatgccgcc gaagatgaca tagtggaagt ggcccacgat
      301 gaagtaggtg tcgtggagct ggacgtcgac cgagagggcc ccgatgaaga ggccggtgag
      361 gccgccgatc gagaagagga acacgaagga cagggcgaag aggaaggggg gctcgagccg
      421 gatcgagccc ttgtacatcg tcgagatcca gttgaagacc ttgatggcgc tcggcaccgc
      481 cacgaggaag gtgaggaggg agaagacgat gcccgcgaag tcgctctggc ccgagacgta
      541 catgtggtgg ccccagacga gggagccgac gctcgcgatc gcgaggctcg agaaggcgat
      601 catcttgtag ccgaagacgt tcttgtggga gaagacgggg aagaggtcgg acatgacgcc
      661 catcgcgggc aggatcatga tgtagaccgc cgggtgggag tagatccaga agaggtgctg
      721 gtagagcagg gggtcgccgc ccttggtggg gtcgaagacc ccgatgccga agagccgctc
      781 cacgacgatg aggagcatcg tgatgccgat gatgggggtc gcgaggatct ggacccaggc
      841 cgtcgagtac aggccccaga ccgagagcgg catgttgaag aagcgcatgc cgggggcccg
      901 cagctggtgg actgtggtga cgatgttgat gccggtgagg atcgaggaca tgccgaggac
      961 aaagaccgcg aaggtggcga ggggcacgtc gaccgtggtc ttgaggctga agggcacgta
     1021 gaaggtccag cccgtgtcgg gggccccgcc gttcacgaag accgaggtga aggcgaggag
     1081 ggccccggcg aggaagaggt accaggtgaa gaggttcagc ctcgggaagg ccatgtcgtt
     1141 ggccccgatg agcatgggga ggcagaagtt gccgaagatc gtgggcaccc cggggatgac
     1201 cacgaggaag accatgatga tcccgtggag ggtgaacagg gcgttgtagg tcgccgggct
     1261 cacgaaggtc gagcccgggt tgaagagggc tatcctcatg aggacgccga ggatcaccgc
     1321 cgccgtgaag aaggtcccga tgaggcagag gtagaggatt ccgatcctct tgtggttggt
     1381 ggcggtgagc cagcccagga tgcccgggta cttggccttc gcgctgaaat agggcccgcc
     1441 gtcgtcgaac tcgcgccgta ccgtgtcgct catcgcactc ccttcctgta gctgcgctgc
     1501 ttccttcccg agaccacgag gtaggccgcg aaggaggcga cgagcagccc gatgacgatc
     1561 ccgctcaccc tcatgaggtc gaaacgcatc tggcggctcc cgggattgta gttgaagcag
     1621 aacctgagca tctttgcaac tgtcggcttc acgatgccct tcgaggcctc gagcaccgac
     1681 atgctgaggt cgaggggcag gaagtctgag ccgttcatgt agcgcacgat cttcccgccc
     1741 ggagcgatga tcacgagccc gagggggtgg tcgaagtcat cgccgtggcg gacgtaggag
     1801 agcccgaccg agtcggccag ggcgtcgatg ctcgcgtcgt cgcccacgag gaagcgccag
     1861 gccgagggcg ggaagggccg ctcgatcgag gcgagggcga tggccttctt ctccaaggcg
     1921 tccttgggcc cctccctgtc gttgacgctc acggtgacga ccgagtagtc gacgccgggg
     1981 accccctgga ggccgcgcag ggccaggccg atccccgtga ggaggacgtt gcactggtcc
     2041 ttgcagcggt agtagacgaa gttgaggacc acgggggtct tcacgagctc ggccatactg
     2101 accgccttgc cgtcctcgcc cgtgaagctg gcgtcgaggg ctatgcggga gccgagcctc
     2161 tcgtcgaagc ccaggggcga ggcgagggcg gccgctgcgg cctggctcgg gaccgtgtgc
     2221 gcgactgcgg cggcgccggc catgaagccg agcagcaagg cgaggggcag ggctctggga
     2281 cctcgactca tcgatgccta cttccaggtc gtcgtcacgt aggcgtagag gtccttggcc
     2341 ttggcgtcgc tcacctggtc ggtaccgaag gggggcatct cgccggcacc gccgttgggc
     2401 atcgtggggt tgcggatgaa ggcggtgaag gcctcgaggg tcttgagctg tttggagccc
     2461 ttgatcggaa ggagggggac gatgatgttc ccgccactcg ggtggcagcc ctggcagttc
     2521 gcgttgaagg tggcctggcc ggcgttgagg tcgccgcccg aggagggggc gcccggcgcc
     2581 gtggcctcgg ccggggcgcc aggggcggcc gcgacgaccg ccttggtctc cgccgtgacc
     2641 gctatgcccc ggccgtagat gatgcccgca ccgaaccagc cgaggcccac gacagctacg
     2701 aaggccaggg cgtagatggc cgtcatccag aaggcgcgga tcttgacctc gctgccgagg
     2761 atgatgcccg ccccgaggag gaggaggacg gtgcccgcga gcaccatctt gatcttgatg
     2821 gcggggaaga ggacggcgtg gtagaagtgg atccagtcga agacgccgaa gaggatggtc
     2881 gggaagacga agaggagggc gaggatcgag gcgtgcctgg ccgaggtgat cagctgccgc
     2941 ctcttgaaga tgatcgcggt gacgaagaag accagggaac cggtgatgag gccgatcggc
     3001 atgtgggtta tcggagcgtg caggggatcg gtgaagccca tcctcgcgag cagggcatac
     3061 atggcatcga gcatcgtgcg cctcctggcg gttatttgca tagggatctt gtgctgtgca
     3121 ggtgcggtcc gtctcctggt gggactggaa ccgtcggttc tatgatacca tgcgccccga
     3181 tgaaacggaa gaagaagctc ggagaaaatg cccggtcggg gctcgcccgg gccctcgccg
     3241 cggtcaagcc cgggagcgtg gccagcaaca gcctgaccgc gatggccggc ttcgccctcg
     3301 ccgcggcgag gtcgaccctc tcccccgccc gggccctcga ggccg
//
LOCUS       DASSQV010000314         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_945735, whole genome shotgun sequence.
ACCESSION   DASSQV010000314 DASSQV010000000
VERSION     DASSQV010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945735"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFL04_05460"
     CDS             <1..578
                     /locus_tag="VFL04_05460"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HET7839186.1"
                     /translation="AAAEAVRAAGKELILHQPMEALGGQDPGPGAIRLSMDRLSVEKT
                     IEANLETVPGAVGMNNHMGSAVTTDPGLMDAVAAVAKERGIYYLDSLTISGTASASAA
                     RREGIRYWERSVFLDNTPDRVSIFRAVDEGKKRSSKGGSAIMIGHVWSAELAQTLMEL
                     YPQLVEEGFSLSTISRFMIQEAEEDDDSPRN"
     gene            559..1614
                     /gene="tsaD"
                     /locus_tag="VFL04_05465"
     CDS             559..1614
                     /gene="tsaD"
                     /locus_tag="VFL04_05465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET7839187.1"
                     /translation="MTVLGIESSCDECAAAVVVDGKTILSDVVASQIPFHTEYQGVVP
                     ELASRMHAEWIYGVARRALEEAGTRPQDLDGIAVTAMPGLLGSLLVGLSFAKGLAWSL
                     GKPYIGVNHILAHLYAPRLEVASPPDYPFLGLIFSGGHSIICRVEDFDRIEVLGTTID
                     DAAGEAFDKVSKHYGFGYPGGAAIDALAAKGDENAFAFPRPTLNKGQRRYDLSYSGLK
                     TAAIRQLEQYRRPGAALSAENIAASFRKAAIDMLADRLLRAARDTGLRTIVAAGGVAA
                     NSYLRSLLGSQPELDVRFPPLRLCGDNGAMVAGIGCKYLERGESSPIDLNVSARVPLY
                     RSPGGREAPAPAPAQDA"
     gene            1670..2239
                     /locus_tag="VFL04_05470"
     CDS             1670..2239
                     /locus_tag="VFL04_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839188.1"
                     /translation="MRRILAFIFTALNLAALCPTAAAGPLGFELRAGPWLGLDRPLRG
                     GSLAAGASLGLGGVVPGLRARMDYDSSLGSLIAGAELSLGFGNEIALVLGGELPLGAL
                     SLETDGARIALTPRSWPNRFGIAAVLAEIAPRRAGRPGLCLETELSWSAYGYADDAQP
                     GLGPEAASGVAGFSAGFRAFLGLVLSWKP"
     gene            2277..>2566
                     /locus_tag="VFL04_05475"
     CDS             2277..>2566
                     /locus_tag="VFL04_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M17"
                     /protein_id="HET7839189.1"
                     /translation="MNTLSGAAAFPQDAALAASASVAVRDVLKVQEGEKALIITNPET
                     EVLRIATALYDALAAAKAKPTLIVQGVKGQMDNAEEAVIAAFESQPDIVMSIS"
BASE COUNT          403 a          959 c          824 g          380 t
ORIGIN      
        1 gggccgcagc cgaggccgtg agggccgcgg gcaaggagct catcctccac cagcccatgg
       61 aggccttagg cggccaggac ccgggcccgg gggcgatcag gctttccatg gaccgactga
      121 gcgtcgagaa gaccatcgag gccaatctcg agacggtccc gggggctgtc ggcatgaaca
      181 accacatggg ctcggcggtc accaccgacc ccggtctcat ggacgcggtc gcggcggttg
      241 caaaagagcg gggaatctac tatcttgact cactcaccat ctccggcacg gcgtccgcct
      301 cggccgcgcg ccgggaggga atccgctatt gggagcgctc cgtgttcctc gacaatacgc
      361 cagacagggt atcgatcttc cgcgccgtgg acgagggcaa gaaacgctcg tccaagggcg
      421 gcagcgcgat catgatcggc cacgtctggt cggccgagct ggcccagacc ctcatggagc
      481 tctatcccca gctcgtcgag gaaggattct ccctctccac catctcgcgc ttcatgatcc
      541 aggaagccga ggaggatgat gacagtcctc ggaattgagt cttcctgcga cgagtgcgca
      601 gccgcggtgg tggtggacgg gaagacgatc ctctccgacg tcgtggcgag ccagatcccc
      661 ttccataccg aataccaggg cgtcgtgccc gagctcgcga gccgcatgca tgccgagtgg
      721 atctacgggg tcgccagacg ggccctcgaa gaggccggca cgaggcccca ggacctcgac
      781 gggatcgccg tcaccgccat gcccggcctc ctgggctccc ttctcgtggg cctctccttc
      841 gccaagggcc tcgcatggtc cctcggcaag ccctacatcg gcgtgaacca catcctcgcc
      901 cacctctacg cgccccggct cgaggtggcc tcaccccccg actacccctt cctcggcctc
      961 atcttctcgg gcggccacag catcatctgc agggtcgagg acttcgaccg gatcgaggtg
     1021 ctcggcacca cgatcgacga cgcggctggc gaggccttcg acaaggtgtc caaacactac
     1081 ggcttcggct atccgggcgg ggcggcgatc gacgccctcg ccgccaaggg tgacgagaac
     1141 gccttcgcct tccccagacc cacactcaac aaggggcaga ggcgctacga cctctcctat
     1201 tcggggctca agacggcggc gatccgccag ctcgagcagt accgcagacc gggggccgcg
     1261 ctgagcgccg agaacatcgc cgcctccttc cgcaaggcgg cgatcgacat gctcgccgac
     1321 cgcctcctca gggcggccag ggacaccggc ctcaggacga tcgtggcggc cgggggcgtg
     1381 gccgccaaca gctatctgcg cagcctcctc ggctcccagc ccgagctcga cgtccgcttc
     1441 ccccccctga ggctctgcgg ggacaacggg gccatggtgg ccggcatcgg ctgcaaatac
     1501 ctggagcggg gcgagagctc gcccatcgac ctgaacgtct cggcgcgggt cccgctctac
     1561 cgcagccccg gcggccgcga agccccggcc cccgcccccg cccaggacgc ctaggccgca
     1621 gctcaagcgc cggtcgcccc cagccgcgtg ctcgggtggg aggcccggca tgcgacgaat
     1681 cctcgccttc atcttcacag ccctcaacct cgcagccctg tgccccaccg ccgcggccgg
     1741 gcccttgggc ttcgagctgc gcgccggccc atggctgggc ctcgacaggc ccttgcgggg
     1801 cgggagcctc gccgcgggcg cgagcctggg gctcggcggc gtcgtgcccg gcctcagggc
     1861 gaggatggac tacgactcct cgctcggaag cctcatcgcg ggggccgagc tctcgctcgg
     1921 cttcggcaac gagatagccc tggtcctcgg cggcgagctc ccgctcggag ccctcagcct
     1981 cgagaccgat ggagctcgga tcgccctcac gccccggtca tggcccaacc gcttcggcat
     2041 cgccgccgtc cttgccgaga tcgctccgcg cagagccggc cgccccgggc tttgcctcga
     2101 gaccgagctc tcgtggagcg cctatggcta tgccgacgat gcccagcccg gcctcggccc
     2161 cgaggcggcg agcggggtcg cgggcttctc ggcgggcttc cgcgccttcc tgggcctcgt
     2221 gctctcgtgg aagccctgac ttgacagaag ggtaaggtcg tagcttagtt gatgcaatga
     2281 acaccctgag cggcgctgcc gcattcccgc aggacgcggc cctcgccgcc tccgcatccg
     2341 tcgccgtccg cgacgtgctc aaggtccagg agggcgagaa ggccctgatc atcaccaacc
     2401 ccgagaccga ggtcctgcgg atcgccaccg ccctctacga cgccctcgcc gcggccaagg
     2461 ccaagccgac cctcatcgtc cagggcgtga agggccagat ggacaacgcc gaggaggccg
     2521 tcatcgccgc cttcgagtcc cagcccgaca tcgtcatgtc gatctc
//
LOCUS       DASSQV010000315         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1939048, whole genome shotgun sequence.
ACCESSION   DASSQV010000315 DASSQV010000000
VERSION     DASSQV010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939048"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..565)
                     /locus_tag="VFL04_05480"
     CDS             complement(86..565)
                     /locus_tag="VFL04_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839190.1"
                     /translation="MGRNADERGRTGMGPLRTLVIALQVLVGANAIAGGWAALSNPRS
                     PFGIPVEALRRGPFQDFLIPGLALVLVLGLGHLASAAMTLFRARLFPYAIAVMGGVTV
                     VWIFVQCYVMETVNPLQAAIFAVGVLEGLYACHLLISGRMFPFSLVWRILGKDGRGR"
     gene            complement(569..949)
                     /locus_tag="VFL04_05485"
     CDS             complement(569..949)
                     /locus_tag="VFL04_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839191.1"
                     /translation="MRRKERDVGISWLELGGKRILYVDYRGINGAEESVRQLREAIEI
                     EKNSGGNLLMLQNYEGTFANEEFVAEIKRLGPQVSDKLAKNAVVGLTGIKKVFLSAYA
                     RISGEKALRAFDTEEQAKAWLVAD"
     gene            1107..2042
                     /locus_tag="VFL04_05490"
     CDS             1107..2042
                     /locus_tag="VFL04_05490"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7839192.1"
                     /translation="MKRIAITMLFLLGLGLIAFADPPAVSSAPALGQNEAFVILNGLP
                     LYQEQGGSLVWKENLVIGDRVLLLNKAQKFKTDGKDRDYLKIRAPSNLEGWVRVQYVV
                     PKSSLAVIKSDKAIVYSEPRDVKITSHYVSNMTIVAVLQDAAAGSFAKLVCYDATQDA
                     YYTDPIYVSRDDLSYADADINSVILYVTAVASKNKDIKSNLLKVIEKRYSASIFFDKI
                     QAVLSSFPAGAASPVKAVQAANGFYTVNDNNVNLRSSPDEVNGPVVGQLRSGTKVEVV
                     EATVQSYTVAGQTGQWYRLKDPAGWVFGSFLSPAP"
BASE COUNT          379 a          695 c          645 g          326 t
ORIGIN      
        1 cgttgggtcc agaccctgta gctgccccgc ggggtgttga cggggatgag cctgacgccg
       61 ggggccggcg tcccgccgct catcatcacc gtccccggcc atccttcccc aggatgcgcc
      121 ataccaggga gaaggggaac atcctccccg agatcagcag gtggcaggcg tagagcccct
      181 cgaggacccc tacggcgaag atcgcggcct ggaggggatt cacggtctcc atgacgtagc
      241 actggacgaa gatccagacg acggtgaccc cgcccatgac ggctatcgca taggggaaga
      301 gcctggcgcg gaagagggtc atcgccgccg aggcgaggtg gcccagcccg agcacgagga
      361 caagggcaag gccggggatg aggaaatcct ggaagggtcc cctgcggagg gcctcgacgg
      421 ggatgccgaa gggggagcgg gggttcgaaa gggcggccca gccccccgcg atggcgttcg
      481 cgcccacgag gacctggagg gcgatgacga gggtccggag cggccccatt ccggtccggc
      541 cgcgctcgtc ggcgtttctg cccatgcgct agtccgccac gagccaggcc ttggcctgct
      601 cctcggtgtc gaaggcgcgc agggccttct cgcccgagat ccgcgcgtag gccgagagga
      661 agaccttctt gatcccggtc aggcccacga cggcgttctt ggctagcttg tcggacacct
      721 gggggccgag cctcttgatc tcggcgacga actcctcgtt cgcgaaggtc ccctcgtagt
      781 tctggagcat gaggaggttc ccgcccgagt tcttctcgat ctcgatggcc tctctgagct
      841 ggcggaccga ctcctcggcc ccgttgatgc cccggtagtc cacgtacagg atccttttgc
      901 cgcccagctc aagccacgat atgcccacgt ccctctcctt ccgcctcatc gggcgccgcg
      961 ccttgcgcgt gagccgaaag tatagatccg gggggctggg ctggcaaacc ctggaaggtc
     1021 cggccggggg aggggctttc gtttgacaag tccggggcgg cgagataccc tcatcgcaaa
     1081 atcattcatc agcaaggagc agccccatga aacggatcgc catcacgatg ctcttcctcc
     1141 tcggcctggg cctcatcgcc ttcgccgatc cgccggcagt cagcagcgcg cccgcgctcg
     1201 gccagaacga agccttcgtc atcctcaacg gcttgcccct ctaccaggag cagggcggca
     1261 gcctcgtctg gaaggagaac ctcgtcatcg gcgacagggt cctgctcctc aacaaggcgc
     1321 agaagttcaa gaccgacggc aaggatcggg actatctcaa gatccgggcc ccgagcaacc
     1381 tcgagggatg ggtgagggtc cagtacgtcg tcccgaagtc gagcctcgcc gtcatcaaga
     1441 gcgacaaggc catcgtctac agcgagcctc gcgacgtcaa gatcacgagc cactatgtct
     1501 cgaacatgac catcgtcgcg gtgctccagg acgccgcggc cgggtccttc gccaagctcg
     1561 tctgctacga cgccacgcag gacgcctact acaccgaccc catctacgtg agcagggacg
     1621 acctgagcta cgccgacgcc gacatcaatt cggtgatcct ctacgtcacg gcggtggcct
     1681 cgaagaacaa ggacatcaag agcaacctcc ttaaggtcat cgagaagcgc tactccgcct
     1741 cgatcttctt cgacaagatc caggctgtcc tctcatcgtt tcccgcgggg gccgcgtccc
     1801 cggtcaaggc cgtgcaggcc gcgaacggct tctacaccgt caacgacaac aacgtgaacc
     1861 tcaggtccag ccccgacgag gtcaacggcc ccgtggtcgg ccagctcaga agcggaacaa
     1921 aggtcgaggt ggtcgaggcc accgtccaga gctacaccgt cgccggccag accggccagt
     1981 ggtacaggct caaggatccc gccggctggg tcttcggcag cttcctcagc ccggcgccct
     2041 gagcc
//
LOCUS       DASSQV010000316         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2189261, whole genome shotgun sequence.
ACCESSION   DASSQV010000316 DASSQV010000000
VERSION     DASSQV010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2189261"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1765
                     /locus_tag="VFL04_05495"
     CDS             182..1765
                     /locus_tag="VFL04_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7839193.1"
                     /translation="METEMLMGAEAIALGALHAGLSAAFAYPGTPSTEIMECLQDIAA
                     REGGFVAQWCSNEKTAFESSLGASYAGRRTMVSMKHVGLNVAADPFVNSALLKVNGGL
                     VVVVADDPGMHSSQDEQDSRWYADFARVPCLEPSDSQEAYDMVREAFELSERKACPVL
                     LRIVTRLAHGRSSVAFSPRIAQKPVSKARDRWGWNLMPAVARKLWSELLGKYEGIRSE
                     IEAGSRIEAGSAELGVITTGLALQYYRENEAELGQRPSHLHIARYPIPHAALRELASR
                     VKRILVLEEGYPFVERHLRGVLDPALPIAGKESGEVPLTGELSPDSIRPALGLPAREG
                     QAGRGLVPPRRPPQLCAGCPHGDSFSFLTEALKGHQVFTVNADIGCYTLGALPPYNAV
                     ETVVEMGASFGMARGASESGLRPAVGVIGDSTFYHSGIPNLVDAVSHGTNVKFMILDN
                     ATTGMTGAQPTIMPSERLKAIALAVGVDPAHVHIVKAHRTEHAANVALLKRELEYEGV
                     SVVIAFRECIEALKKARKA"
     gene            1762..>2206
                     /locus_tag="VFL04_05500"
     CDS             1762..>2206
                     /locus_tag="VFL04_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HET7839194.1"
                     /translation="MKYDIILCGVGGQGGISVSVVIARAALAEGLQVKQSEVHGMSQR
                     GGEVLAHLRISDTAIASPTIPLGTARLVLAFEPLEALRYLPWLSREGSVISATEPIRN
                     IQDYPELEAVHAELRALPKALLVDADRIAKEAGNARAANMVLVGAA"
BASE COUNT          361 a          837 c          698 g          310 t
ORIGIN      
        1 ctccgcggcc tcgggaagga caacgcctac ctccacccgg tcttccccaa ggagctgatc
       61 gaggactgga tcgccctcaa ggagaaggaa gccgaatacg tgtacaacgc gcccgtgccc
      121 caggagtacg agctcaattt ctgacagcct gcgcggcaaa cgcgcagcca agggaagcag
      181 catggaaact gaaatgctca tgggggccga ggccatcgct ctgggcgccc tgcacgcagg
      241 gctctcggcg gccttcgcct atcccgggac cccctcgacc gagatcatgg agtgcctcca
      301 ggacatcgcc gcccgcgagg gcggcttcgt ggcccagtgg tgctcgaacg agaagacggc
      361 cttcgagtcc tcgctcgggg cctcctacgc gggcaggcgg acaatggtgt cgatgaagca
      421 cgtcggcctc aacgtcgccg ccgacccctt cgtgaacagc gccctgctca aggtcaacgg
      481 cggcctcgtc gtggtcgtcg ccgacgaccc cgggatgcac tcctcccagg acgagcagga
      541 ctcgagatgg tacgccgact tcgcgcgcgt gccctgtctc gagccctccg attcgcagga
      601 ggcctacgac atggtccgcg aggccttcga gctctcggag cggaaggcct gccccgtact
      661 cctgcggatc gtcacgaggc tcgcccacgg gcgctcctcc gtggccttct cgccgcgcat
      721 cgcgcagaag cccgtgtcca aggcccggga ccgctggggc tggaacctca tgcctgcggt
      781 ggcgaggaag ctctggtcag agctcctcgg gaaatacgag ggcatccgca gcgagatcga
      841 ggccggctcc agaatcgagg ccggatcggc cgagctcggg gtcatcacga cgggcctcgc
      901 cctccagtac taccgcgaga acgaggccga gctcggccag aggccgagcc acctccacat
      961 cgcccgctac cccatccccc acgcggccct gcgcgagctc gcctcccggg tcaaacgcat
     1021 cctcgtgctc gaggagggct atcccttcgt ggagcgccac ctgcgaggcg tcctcgaccc
     1081 cgccctgccc atcgccggca aggagagcgg cgaggtcccc ctcaccgggg agctcagccc
     1141 cgacagcatc cgccccgccc tcggcctccc cgccagggaa gggcaggccg gccgcggcct
     1201 cgtgccgccc aggcgcccgc cccagctctg cgccggctgc ccccacggcg actccttctc
     1261 cttcctcacc gaggccctca agggccacca ggtcttcacg gtgaacgccg acatcggctg
     1321 ctacaccctc ggcgccctcc cgccctacaa cgccgtggag acggtcgtgg agatgggcgc
     1381 ctccttcggc atggcccgcg gcgcctccga gtcgggcctc aggccggccg tcggggtgat
     1441 cggtgactcg accttctacc actcgggcat accgaacctg gtcgacgccg tctcgcacgg
     1501 gaccaacgtg aagttcatga tcctggacaa cgccacgacg ggcatgacgg gcgcccagcc
     1561 gaccatcatg ccctcggagc ggctcaaggc catcgccctc gcagtcggcg tcgatcccgc
     1621 ccacgtccac atcgtgaagg cgcaccggac cgagcacgcc gccaacgtcg ccctcctgaa
     1681 gcgagagctc gagtacgagg gcgtgtcggt ggtcatcgcc ttccgcgagt gcatcgaggc
     1741 cctgaagaaa gcgaggaagg catgaagtac gacatcatcc tctgcggagt cggcggccag
     1801 ggcggcatct ccgtgtcggt cgtgatcgcc cgcgcggccc tggccgaggg cctccaggta
     1861 aagcagtccg aggtccatgg catgtcccag cgggggggcg aggtcctcgc ccacctccgc
     1921 atctcggaca cggcgatagc gagccccacg atccccctgg gcacggccag gctcgtcctc
     1981 gccttcgagc ccctcgaggc cctgcgctac ctgccctggc tgtcgcgcga gggctccgtg
     2041 atctcggcca cagagccgat ccggaacatc caggattacc ccgagctcga ggcggtccac
     2101 gccgagctcc gcgccctgcc caaagccctc ctcgtcgacg ccgaccgcat cgccaaggag
     2161 gcgggcaacg cgcgcgccgc caacatggtc ctcgtcggcg cggccg
//
LOCUS       DASSQV010000317         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_572921, whole genome shotgun sequence.
ACCESSION   DASSQV010000317 DASSQV010000000
VERSION     DASSQV010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572921"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..374)
                     /locus_tag="VFL04_05505"
     CDS             complement(159..374)
                     /locus_tag="VFL04_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7839195.1"
                     /translation="MEQRTVGNNFRSFRQKANLTQEEVAKAIGVTRQTVLAIEKGNYV
                     PSVLLALVSARLFGCAVEELFYVDEPR"
     gene            591..791
                     /locus_tag="VFL04_05510"
     CDS             591..791
                     /locus_tag="VFL04_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839196.1"
                     /translation="MKLVLNILGILCLPVGAIWTLQGFNIMRGSVMSGHRRWIAIGLV
                     LLVAGVIILILNNRRRGAARLP"
     gene            867..2387
                     /locus_tag="VFL04_05515"
     CDS             867..2387
                     /locus_tag="VFL04_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7839197.1"
                     /translation="MNIAQERLVEDQVREDYLADFAARLRHILHARGDLDFLSLQRGI
                     PPYVFHEVQALRPLAGSIPIEHGGRGAEPKEILSVLEASSYESLPLGLVLAINGALFL
                     EPLAKYGSEGLKASVFPRFVEEGAMGGLMITEPDYGTDALSMQTSFEEEGGGYAIRGT
                     KHWAGLSGWADFWIITARRRRETGALQRDIDFFVCDSRQPGQVVSVEEWYPNLGLYMI
                     PYGRNRVDVRVPLGARLAPVSNGLGLLRDLLHRSRMRFAGMAVGFIRRILDEAIEHCR
                     ERKVGGRILSSFDQVRRRLAEIQAGHTISAAFCRHSSEVSGIGNDLADEGLAANVHKT
                     VLSDLMQQASQSFLQLMGAKGYRLDHLAGRAVVDSRPFQIFEGSNDVMYDQIAAAFLK
                     RMRQIGESSLLGLLRLHELTSLAAERFTRILDFQVAGELIQRKMVDLGRIIARVVSVE
                     FLLRLGSGGYDAGLIENAIEVLREKVGMIAAGFREGAAVPLVEDYAGARAWQACTP"
     gene            complement(2434..2937)
                     /locus_tag="VFL04_05520"
     CDS             complement(2434..2937)
                     /locus_tag="VFL04_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7839198.1"
                     /translation="MANTRVRFETSLGDILIELFDEQVPITVKNFLSYVGSGFYDGTI
                     FHRVIPGFVVQGGGMLPGMDRKETEDPIVNEAAKGPRNSRGTLSMARTSDPHSATSQF
                     FVSLVDNRSLDYSGPSAQGAGYCVFGRVVEGMEVVDAMATKPTTRRPPHADVPAEDIV
                     LKKASLA"
     gene            complement(2988..3602)
                     /locus_tag="VFL04_05525"
     CDS             complement(2988..3602)
                     /locus_tag="VFL04_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET7839199.1"
                     /translation="MRKSAVVTAVLIAAAASLAGAQGFGLSTTALVGAGETGFDSGIK
                     ASGSLDGAYAAKPGAPGNPRSFPFSWSNLPTGTKALALVLDDPDARLVLAAYGMKGDA
                     FLHWIAADIDPGLGGLGDNASATAAFPQGKNGAGTIGYTGPQPPTDLPKEVQGRRIHV
                     YRLKVFALSSPTGLKNGFSLDELLAAVKGRTLGTAELDLSYSNQ"
BASE COUNT          611 a         1192 c         1320 g          653 t
ORIGIN      
        1 cgtcgcgcgc cctctgccgg aagaggaccc ccgcgtagag cccggcggcg gccactagca
       61 aggcgaggat gctcatctgg acctggttcg gcatcagcat gccgaagggg tcgagcttca
      121 ggacgacaag ggcgatgaac acgagcgaca cggccagttc agcgaggttc gtcaacatag
      181 aagagctcct ctacggcgca gccgaacagc ctggcgctca cgagcgccag caggacggag
      241 ggcacgtagt ttcccttttc gatggcgagc accgtctggc gggtcacccc gatcgccttc
      301 gccacctcct cctgcgtgag gttcgccttc tggcgaaagc tcctgaagtt gttgccgacc
      361 gtcctctgtt ccacaagcga aatgtatggt tggctttaca ataagtcaag ttgatttgac
      421 atgcgatgga aaggcctggg cccgataccc gccttaggag acgagcagcg gccaccacca
      481 ccgacctgct tggcccttac ccaatccgct tgccgtcccg ggatcccctt ggcatcttca
      541 tctggacagc ggcattgccg cgatggccga tgccaaaaag gaggtggggc atgaagcttg
      601 tcctgaatat actcgggatc ctgtgcctgc ccgtcggggc catctggacc ctccagggct
      661 tcaacatcat gcgcgggagc gtgatgtcgg gtcatcggcg ctggatcgcc atcggcctgg
      721 tcctgctggt cgctggggtc atcatcctga tcctgaacaa ccggagaagg ggcgcggccc
      781 gcctgccctg atcagagcac aggctgtccg cgggcgccgc ttcggaccgc ttgcgcggcc
      841 attaaagatg tgcaatatag taatctatga atattgcaca agaacggctt gtcgaggacc
      901 aggtccgaga ggactacctc gccgacttcg ccgcaaggct gcggcatata ctccatgcgc
      961 gcggagacct ggatttcctg agccttcagc gcgggatacc cccctatgtc ttccacgagg
     1021 tgcaggccct gaggcccctg gcgggctcaa tccccatcga gcacggaggg aggggcgccg
     1081 aaccgaagga gatcctctcg gtcctcgagg cgagctctta cgagtccctc cccctcggcc
     1141 tcgtgctcgc gatcaacggg gcccttttcc tcgagcccct cgccaaatac ggctcggagg
     1201 ggctcaaggc cagcgtattc ccgcgctttg tcgaggaggg ggccatgggg gggctcatga
     1261 tcaccgagcc ggactatggc accgacgccc tctcgatgca gaccagcttc gaggaggagg
     1321 gcgggggcta cgccatcagg gggacgaagc actgggcggg gctctcgggc tgggccgatt
     1381 tctggatcat cacggcgagg cggaggaggg agacgggggc tctccagcgg gacatcgact
     1441 tcttcgtctg cgattcgcgg cagcccggtc aggtggtctc ggtcgaggag tggtacccga
     1501 acctcggcct gtacatgatc ccctatggcc ggaaccgggt cgacgtcagg gtgcccctcg
     1561 gcgcgaggct cgcccccgtc tcgaacggcc tgggcctgct ccgcgacctc ctgcaccgca
     1621 gccgcatgcg cttcgccggc atggctgtgg gctttatccg caggatccta gacgaggcga
     1681 tcgagcactg ccgcgagcgg aaggtcggcg gcaggatcct gtcgtccttc gaccaggtca
     1741 ggaggaggct ggcggagatc caggccgggc atacgataag cgcggccttc tgccggcatt
     1801 ccagcgaggt ctcgggcatc gggaacgacc tggccgatga gggcctcgcc gccaacgtgc
     1861 acaagacggt gttgagcgac ctgatgcagc aggcctcgca gtccttcctc cagctcatgg
     1921 gggccaaggg ctaccgcctc gaccacctgg ccggcagggc cgtcgtcgac tcccgcccct
     1981 tccagatctt cgagggctcg aacgacgtga tgtacgacca gatcgccgcg gccttcctga
     2041 agcgcatgag gcagatcggg gagtccagcc tcctcggcct cctgaggctg cacgagctca
     2101 cgagcctggc cgccgagcgc ttcacccgga tcctcgactt ccaggtcgcg ggggaactca
     2161 tccagcgcaa gatggtcgac ctcgggagga tcatcgcccg ggtggtctcg gtggagttcc
     2221 tcctcaggct gggctcgggg ggctacgacg cgggcctcat cgagaacgcg atcgaggtgc
     2281 ttagggagaa ggtagggatg atcgccgcgg gtttccgcga gggcgccgcc gtgcccctcg
     2341 tcgaggacta cgcgggcgcc cgcgcctggc aggcctgcac gccctaggac acccaggccc
     2401 cgccgcccca aggggggcgg gggcccgtgg ggttcaggcg aggctcgcct ttttgagcac
     2461 gatgtcctcg gccggcacgt cggcgtgggg gggccttcgc gtggtcggct tcgtcgccat
     2521 cgcgtcgacg acttccatgc cctcgacgac cctgccgaag acgcagtagc ccgcgccctg
     2581 ggcgctgggg cccgagtagt cgaggctccg gttgtccacg aggctcacga agaactggct
     2641 cgtcgcgctg tggggatcag aggtcctcgc catggagagg gtgcccctgc tgttgcgcgg
     2701 gcccttggcg gcctcgttca ctatcggatc ctcggtctcc ttgcggtcca tgccggggag
     2761 catgcccccg ccctggacca cgaagccggg gatcacgcga tggaagatgg tgccgtcata
     2821 gaagcccgag ccgacgtagg aaaggaaatt cttcaccgtg atggggacct gctcgtcgaa
     2881 aagctcgatc aggatgtctc cgaggctcgt ctcgaagcgt acccgggtgt ttgccatgtg
     2941 ttcctccgcg cgtgagagta taccgggcag gtcctcttgc gacagcccta ctggttcgag
     3001 tagctcaggt ccagctccgc ggttccgagg gtccggcctt tgacggcggc caggagctcg
     3061 tcgaggctga agccgttctt gagccccgtg ggggaggaga gggcgaagac cttgaggcgg
     3121 tagacgtgga tccttctgcc ctggacctcc ttgggcaggt cggtcggggg ctggggacct
     3181 gtgtagccta tcgtgccggc cccgttcttg ccttggggga aggccgccgt ggccgaggcg
     3241 ttgtcgccga ggccgcccag gccagggtcg atgtcggcgg cgatccagtg gaggaaggcg
     3301 tcgcccttca tcccgtaggc cgccaggacg aggcgggcgt cggggtcgtc gagcacgagg
     3361 gcgagggcct tggtgcccgt ggggagattg gaccaggaga aggggaagga gcggggattg
     3421 cctggagccc ccggcttggc ggcgtaggct ccatcgaggc tccccgaggc cttgatgccg
     3481 ctgtcgaagc ccgtctcgcc cgctccgacg agggccgtcg tgctgaggcc gaagccctgc
     3541 gcgcccgcga gcgaggccgc ggcggctatc agcacggccg tgaccactgc actttttctc
     3601 atgtctgcct ccttgggaca tggcgactat ccagaaaaga tactgccggc ggcggcaggg
     3661 ggcgaggcgg gcaggcggcg ggttggctgg ctgaaggctg gctgaaggct ggcggaaggt
     3721 tggcggaagg ttggcggaag gttggcgggg ctggcgggcg gcgcgagcca gaagcc
//
LOCUS       DASSQV010000318         7189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_449581, whole genome shotgun sequence.
ACCESSION   DASSQV010000318 DASSQV010000000
VERSION     DASSQV010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449581"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="VFL04_05530"
     CDS             <1..1462
                     /locus_tag="VFL04_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7839200.1"
                     /translation="TPGYDPRLRPWYDTAMKAPADRSRTDARLTSDGRLAITLLERVR
                     DPAGKVSGVATLSISLENLSALISSLRIGERGYVILVQADGTILADPRHPENNFKKVG
                     ELKEPGYARAFAAGDAGTRIGLGGTEYGTVARRFASLGFTGIGLLDRGELSSRLISVL
                     SLVAIIAACSALAAGLAGGLIAARLARPMRAAAVMAETIAGGALELHQDSADLARRDE
                     IGALSRSFRTMAGRLAQIVGGIRSAATNASAGATQISGTAQALAEGTTEQAASAQEVS
                     ASIAQMAATIAHNAELSNDTERMARQAAQGMTEGGQAIIEAVAAMRQIASSISIIEEI
                     ARQTNLLALNAAIEAARAGEAGRGFAVVATEVRKLAERSQTAAGEIAVLSRDSVSVAA
                     RAMELLERISPEVARTAERMLEIAAASREQSTGADQVAKAVSQLDSVIQRNAAASEEL
                     ASSAVELSGQAHSLEDAVSYFRLSGEADPTRLLDAP"
     gene            complement(1485..2345)
                     /locus_tag="VFL04_05535"
     CDS             complement(1485..2345)
                     /locus_tag="VFL04_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET7839201.1"
                     /translation="MRGKAERGPQETVPRIDEARLAEFRDLVMEHYRRAGRSFPWRDT
                     RDPWSILVSEVMLQQTQTIRVVPKYEEWTRRWKGPAELAAAPLAEVLGLWSGLGYNRR
                     ALALVRAAGAVVRDHGGILPQDEASLLELPGVGSYTARAVLAFAFDRPVVFVETNIRA
                     AFIHHFFGAMERVPDSLILPLVEGSLDRDDPRIWYYALMDYGAELKRTAGNASRRSAH
                     YAKQSPFQDSHRRVRGAVLKALASGGEIGREELAASLPFARERVERAAAELVAEGFAE
                     YRGEALRIRR"
     gene            complement(2342..4528)
                     /locus_tag="VFL04_05540"
     CDS             complement(2342..4528)
                     /locus_tag="VFL04_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7839202.1"
                     /translation="MMKVFLAALLALALGHAFAEGFGLPLADWRWTSGDDPAYARPDF
                     DDSSWQSLSLPGQIRPESLGGVFWLRAKVEIPATAPERLWLLTGKSGVALEAFVGGQY
                     AGARGSLPPAFELRATHASAILLPANAAKPGGQAVLALRCAYLGTTPRIPLYRLGDAA
                     AAAFELGPVNFWNGRLYAILSSLCFFLGFYFLLLFAFRASGRENLYYALTLIFIALYF
                     LEIGAENAPFRGPWFRGLARSGLVLSMSFLVPFLGEFFALRSSRLLRTACLAAGAGFT
                     VFFLAYSGDDSALNLIFTLSLLPVTAEILYGLYATIKALRAGAAEAWPVLAGIGIGVV
                     LSAYDVSYQVSGAEPFAWLQGIAFFALNISIFVALSMRSGRLKLDLERYAREVEGKQA
                     ELSEYVKRLVDAGEAAARISEELDIAAAKAARGAESAAKGSALIESQASGQADSARQA
                     NELVSGLVASIGKVSASLGGQTENVQRTASAVVELSAGAEAVAANIERTGSFAESLAA
                     LTGSGERAAAALVAAMEGVSSASEGIGEVVDAVNEFAERTNLLAMNAAIEASHSGASG
                     RGFTVIAGEVKKLAAAQSERAARIKDRVAEIGMRVRDGSVQAERVRSALRDIAAGAVE
                     AAARIGEVRAGTGEQKRAISEIGGSMEALSRSGSAIREEAERQAAYSAKVRDTVAAMA
                     ADATEVRASAVAIAEEGAVLVDAVRRLGELAARGRELNSSLGSTGS"
     gene            complement(4628..5230)
                     /locus_tag="VFL04_05545"
     CDS             complement(4628..5230)
                     /locus_tag="VFL04_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET7839203.1"
                     /translation="MSDAFQWLYSDIVKEHFQNPKYVFDPDEKFEADAEGVVGNIKCG
                     DQMMFMLKIKDDVISDVRWRTYGCASAIASTSMLSTVIVGMPIREAYGIKPADIARRL
                     GGLPENKIHCSVLGDKALRAAIDSYLDKRGRSGEFKGEDAVVVCTCLNITDKDIEQAV
                     KDGARSWDALQAATKIGTVCGGCKGKAEELLHEFAHIYGD"
     gene            complement(5268..6476)
                     /locus_tag="VFL04_05550"
     CDS             complement(5268..6476)
                     /locus_tag="VFL04_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET7839204.1"
                     /translation="MDKRFVYLDYNATTPLRGEVKARLVEGLEVFANASSMHEAGRRA
                     RDRVERARASVAALIGARDPLNVYFTSGGSESNNTVFQTMAELVRDPARSSRRKIITS
                     AIEHPCVLNSAAHLRDLGFPVVFLGVDAEGRIDLEALKAELDPSGPGGPTLLVSIMAA
                     NNEIGTLQDMKEITRLAKAAGALVHSDAVQAAGKVAVDVEAWGLDYLTLSCHKLYGPK
                     GIGALYVRKGAPIEPLIRGGHQELGVRAGTYNNLGIIGFGLAAELAQKELPEYQTRVG
                     GLRERLRAGIVAAVPQVHVNGHHDQVLPNTLNLSFPGAEGEAILLSLDLEGIEVSTGS
                     ACASGSLEPSHVLMATGVGPELAHGSIRFSLGRDTSEADIDYVIQKLPPIITRLRRMS
                     TLNPVAAACV"
     gene            complement(6489..6890)
                     /locus_tag="VFL04_05555"
     CDS             complement(6489..6890)
                     /locus_tag="VFL04_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET7839205.1"
                     /translation="MFSVPTRTQYGIRALVHLATSEARSSTSAEIARAQTISPKYLEG
                     ILGQLKASGLVLSERGAHGGYRLARGPEQILMREIVEALEGEVRPVGCVDDSDCCEQG
                     VDCLPRRFWIGLKQAIDAYLDSKTLRDVIEA"
     gene            complement(6960..>7189)
                     /locus_tag="VFL04_05560"
     CDS             complement(6960..>7189)
                     /locus_tag="VFL04_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839206.1"
                     /translation="DENHRVLHDYLESDLERSRSGDIHQKHYLWYQWRQGLLTISGAD
                     SEAKDDFEAFQQWCEQPEHRLLILGRMRKLH"
BASE COUNT         1092 a         2448 c         2567 g         1082 t
ORIGIN      
        1 gaccccgggc tatgaccccc ggctccggcc ctggtacgac acggcgatga aggccccggc
       61 cgaccgatcc cgtaccgacg caaggctcac ctccgacggc aggctcgcca tcaccctcct
      121 tgagcgggtc cgcgatcccg cgggcaaggt cagcggggtc gcgaccctct cgatatccct
      181 ggaaaacctc tcggccctca tctcctcgct tcggatcgga gagcggggct acgtcatcct
      241 cgtccaggcc gacgggacga tcctcgccga cccgaggcat ccggagaaca acttcaagaa
      301 ggtgggcgaa ctcaaggagc cgggctacgc gagggcattc gcggcagggg atgcgggaac
      361 gaggatcggc ctgggcggca ccgagtacgg caccgtcgcg aggcgcttcg ccagcctcgg
      421 gttcaccgga atcgggcttc tcgacagggg cgaactcagc tcccgcctga tctcggtcct
      481 gtccctcgtc gcgatcatcg ccgcatgctc ggccctcgcg gcaggcctcg ccggcgggct
      541 catcgccgcc aggctcgcgc ggcccatgcg ggccgcggca gtaatggccg agaccatagc
      601 cggaggtgcc ctcgagctgc accaggactc ggcagacctc gcgcggaggg acgagatcgg
      661 cgccctgtcc cgctccttca ggacgatggc cggacgcctc gcccagatcg tcggcggaat
      721 caggagcgcg gccaccaacg cctcggccgg ggccacgcag atcagcggca cggcccaggc
      781 cctcgcggag gggacgaccg agcaggcggc cagcgcccag gaggtctcgg cctcgatcgc
      841 ccagatggcg gccacgatag cgcacaacgc ggagttgtcc aacgacactg agaggatggc
      901 gcggcaggcc gcccagggga tgaccgaggg cgggcaggcc atcatcgagg ccgtcgcggc
      961 catgaggcag atcgcctcga gcatctccat catcgaggag atcgcgaggc agaccaacct
     1021 cctcgccctg aacgccgcga tcgaggcggc gagggcgggc gaggcgggca ggggcttcgc
     1081 cgtggtcgcc accgaggtcc gcaagctcgc cgagcgctcc cagaccgccg cgggggagat
     1141 cgcagtgctg tcgcgcgact ccgtctcggt ggccgcgagg gcgatggagc tcctcgagcg
     1201 gatcagcccc gaggtggcga ggacggccga gcggatgctc gagatcgcgg ccgccagccg
     1261 cgaacagagc accggcgccg accaggtggc caaggccgtg agccagctcg actcggtcat
     1321 ccagaggaac gctgcggcct ccgaggagct cgcctcctcg gccgtggagc tctcgggcca
     1381 ggcccacagc ctcgaggacg cggtgagcta tttcaggctg tccggagagg ccgatcctac
     1441 ccggctcctg gacgcgccct aggccttccg cgcggggagc ggcctcatcg cctgatgcgc
     1501 agcgcctcgc cccggtactc ggcgaagccc tcggccacca gctcagcggc cgccctttcc
     1561 acacgctcgc gggcgaaggg cagggaggcc gcgagctcct ccctgccgat ctctccgcct
     1621 gaggcgaggg ccttgagcac ggcccccctc accctgcggt gggaatcctg gaaggggctc
     1681 tgtttcgcgt agtgcgcgct ccgcctcgag gcgttgcccg ccgtcctctt gagctcggcc
     1741 ccgtagtcca tgagggcata gtaccagatc ctcggatcat ccctgtccag gctcccctcg
     1801 acgaggggga ggatgagcga gtcgggcacc ctttccatgg ccccgaagaa gtggtggatg
     1861 aaggccgcgc ggatgttggt ctcgacgaag actacgggcc ggtcgaaggc gaaggccagg
     1921 acagccctcg ccgtgtagct cccgactccc ggcagctcga ggagggaggc ctcgtcttgg
     1981 ggcaggatcc ccccatggtc cctgacgacc gccccggccg cgcggacaag ggccaaggcg
     2041 cggcggttgt agccaaggcc gctccagagg cccaggacct cggccagggg ggccgccgca
     2101 agctcggccg ggcccttcca gcggcgagtc cactcctcgt acttcgggac gacgcgaatc
     2161 gtctgggtct gctggagcat cacctcggac acgagtatcg accagggatc ccgggtatcc
     2221 ctccagggga agctccggcc cgcgcggcgg tagtgctcca tgacgaggtc cctgaactcg
     2281 gcgagccggg cctcatcgat ccgggggaca gtctcctgcg gcccccgctc ggccttcccc
     2341 ctcacgatcc cgtggagccc aggctggagt tgagctccct gcccctcgcg gccagctcgc
     2401 cgagccgcct cacggcgtcg accaggacgg cgccctcctc ggcgatcgcg acggccgagg
     2461 ccctcacctc ggtcgcgtcg gcggccatgg cggcgaccgt gtcgcgcacc ttggcggaat
     2521 aggccgcctg gcgctcggcc tcctccctga tcgccgagcc cgagcgcgag agggcctcca
     2581 tagagccgcc tatctccgag atcgcgcgct tctgctcccc ggtgccggcc ctgacctcgc
     2641 cgatcctggc cgcggcctcg accgccccgg cggctatgtc gcgcaacgcg gagcgcacgc
     2701 gctcggcctg caccgagccg tcgcggaccc tcatcccgat ctcggccacc ctgtccttga
     2761 tccgcgccgc gcgctccgac tgcgcggccg cgagcttctt cacctcgccc gcgatgacgg
     2821 tgaagccccg gcccgaggcc cccgagtgcg aggcctcaat ggcggcgttc atcgcgagga
     2881 ggttggtgcg ctcggcgaac tcgttcacgg cgtccaccac ctcgccgatg ccctcggagg
     2941 cgctcgacac gccctccatc gcggccacga gggccgcggc ggcgcgctcc cccgagccgg
     3001 tcagcgcggc gaggctctcg gcgaaggagc ccgtcctctc gatgttggcg gccacggcct
     3061 cggccccggc cgagagctcg acgacagccg aggcagtgcg ctggacgttc tccgtctggc
     3121 cgccgagcga ggcgctcacc ttgccgatcg aggcgacgag gccggacacg agctcgttgg
     3181 cctgtctcgc ggaatcggcc tggcccgagg cctgggactc gataagggcc gagcccttcg
     3241 ccgccgactc ggccccgcgc gccgccttgg ccgccgcgat gtcgagctcc tcggagatcc
     3301 gcgccgcggc ctcccccgcg tcgacgaggc gcttgacgta ctccgagagc tcggcctgct
     3361 tgccctcgac ctcgcgggcg tagcgctcga ggtccagctt gagcctgccc gagcgcatcg
     3421 agagggcaac gaagatggag atgttgaggg cgaagaatgc gatgccctgg agccaggcga
     3481 agggctcggc cccggagacc tggtagctca cgtcgtaggc cgagagcacg acgccgatgc
     3541 ctatcccggc gaggaccggc caggcctcag ccgcgccggc cctgagggcc tttatcgtcg
     3601 cgtagaggcc gtagaggatc tcggcggtca cgggcaggag ggagagggtg aagatgaggt
     3661 tgagcgcgct gtcgtcgccc gagtaggcga ggaagaagac ggtgaagccg gcccccgcgg
     3721 cgaggcaggc cgtccgcagc agcctggagc tccggagcgc gaagaactcg ccgaggaagg
     3781 gcacgaggaa ggacatggag agcacgaggc ccgagcgcgc gaggccgcgg aaccagggcc
     3841 cgcggaaggg ggcgttctcg gccccgatct cgaggaagta cagggctatg aagatgaggg
     3901 tcagggcgta gtagaggttc tctcggcccg aggccctgaa ggcaaagagg aggaggaagt
     3961 agaagccgag gaagaagcag aggctcgaga ggatggcgta gagcctcccg ttccagaagt
     4021 tgacggggcc gagctcgaag gccgccgccg cggcgtcgcc caggcggtaa agcgggatgc
     4081 gcggggtcgt gccgagatag gcgcagcgca gggccaggac ggcctggccg ccgggcttgg
     4141 cggcattcgc cgggaggagg atggccgagg cgtgggtggc ccggagctcg aaggccggcg
     4201 gcagcgagcc cctggcgccc gcgtactggc cgccgacgaa ggcctcgagg gcgacgcccg
     4261 acttgccggt gaggagccag agcctctcgg gcgcggtggc ggggatctcg accttggccc
     4321 tgagccagaa gaccccaccc aggctctccg gcctgatctg gcccgggagg ctcagactct
     4381 gccacgagga gtcgtcgaag tcgggtcggg cgtaggcggg gtcatcgccc gaagtccagc
     4441 gccagtcggc cagggggagg ccgaagcctt cggcgaaggc atggccgagg gccagggcga
     4501 gcagggcggc gaggaatact ttcatcattg ggcgggccat agactcccat cgtaacgcct
     4561 cggcccgccc gcttcaaggg aaaggcccta aagcgggcga tccccgcgat gcccggaccc
     4621 gcgcctttca gtcgccgtag atgtgggcga actcgtggag gagctcctcg gccttgccct
     4681 tgcagccgcc gcagacggtg ccgatcttgg tggcggcctg gagggcatcc cagctgcggg
     4741 ccccgtcctt caccgcctgc tcgatgtcct tgtcggtgat gttgaggcag gtgcagacca
     4801 cgacggcgtc ctcgcccttg aactcgccgg agcggcccct cttgtcgagg taggagtcga
     4861 tcgccgcgcg gagggccttg tcgccgagga ccgagcagtg gatcttgttc tcgggcaggc
     4921 cgccgagccg cctcgcgatg tcggcgggct ttatgccgta ggcctcgcgg atgggcatgc
     4981 cgacgatcac cgtggacagc atcgaggtgc tcgcgatggc gctcgcgcag ccgtaggtcc
     5041 tccacctcac gtcgctgatc acatcgtcct tgatcttgag catgaacatc atctggtcgc
     5101 cgcacttgat gttgccgacg acgccctcgg cgtccgcctc aaacttctcg tcggggtcga
     5161 agacgtactt ggggttctgg aagtgctcct tcacgatatc ggaatagagc cactggaagg
     5221 catcgctcat gtgctgctcc tgtcttcggg tctgttcttc gtcctcgtca gacgcaggcc
     5281 gccgccaccg ggttgagggt ggacatgcgg cgcaggcggg tgatgatggg cggcagtttc
     5341 tgtatcacgt agtcgatgtc ggcctcgctc gtgtcccgtc cgaggctgaa gcggatcgag
     5401 ccgtgggcga gctcgggccc gactccggtg gccatgagga catggctggg ctcgaggctc
     5461 cccgaggcgc aggccgagcc cgtcgagacc tcgatgccct cgaggtcgag ggagaggagg
     5521 atggcctccc cctcggcccc ggggaaggac aggttgagcg tattcgggag gacctggtca
     5581 tggtggccgt tcacgtggac ctggggcacg gccgcgacga tcccggcgcg gagcctctcg
     5641 cggaggcctc ccacgcgggt ctggtattcg gggagctcct tctgggcgag ctcggccgca
     5701 aggccgaagc cgatgatgcc gaggttgttg taggtgccgg cgcgcacgcc gagctcctgg
     5761 tggccgcccc tgatgagggg ctcgatcggc gcccccttgc ggacgtagag ggctccgatg
     5821 cccttggggc cgtagagctt gtggcaggag agggtgaggt agtcgaggcc ccaggcctcg
     5881 acgtccacgg cgaccttgcc cgcggcctgg accgcgtcgc tgtggacgag ggcgccggcg
     5941 gccttggcga gccgggtgat ctccttcatg tcctggaggg tgccgatctc gttgttggcc
     6001 gccatgatgg agacgaggag ggtcgggccg ccgggtccgg aggggtcgag ctcggccttc
     6061 agggcctcga ggtcgatcct gccctcggcg tcgaccccga ggaagacgac gggaaaaccg
     6121 aggtcgcgca gatgggcggc cgaattgagg acacaggggt gctcgatggc gctcgtgatg
     6181 atcttgcgcc tcgaggacct cgcggggtcg cggacgagct cggccatcgt ctggaagacc
     6241 gtgttgttcg actccgagcc gcccgaggtg aagtagacgt tgaggggatc cctcgccccg
     6301 atgagggcgg cgacgctggc cctcgccctc tcgacgcggt ctcgggccct gcgcccggcc
     6361 tcgtgcatgc tcgatgcgtt cgcgaagacc tcgagcccct ccacaagcct ggccttgacc
     6421 tcgccgcgca ggggcgtcgt cgcgttgtag tcgagataca cgaatcgctt gtccatatcc
     6481 tctctccttc aggcctcgat gacgtcgcgg agggtcttcg aatccaggta ggcgtcgatc
     6541 gcctgtttca ggccgatcca gaagcgccgc ggaaggcagt cgaccccctg ctcgcagcag
     6601 tccgagtcgt cgacgcagcc gacgggcctg acctcgccct cgagggcctc gacgatctcc
     6661 cgcatgagga tctgctcggg cccgcgggcg aggcggtagc ccccgtgggc cccccgctcg
     6721 ctcaggacga ggcccgaggc cttcagctgc ccgaggatgc cctcgaggta cttcggcgag
     6781 atcgtctggg ccctggcgat ctcggcgctc gtcgaggagc gcgcttcgct ggtcgccagg
     6841 tggacgaggg ctctgatgcc gtactgggtc cttgtcggta ccgagaacat ctatactccg
     6901 gtattcctat cgggatagta ggaatatacc cgcccggcgg gtacatggtc aagccgggcc
     6961 tagtggagct tcctcatccg ccccaggatg aggagccggt gctcgggctg ctcgcaccac
     7021 tgctggaagg cctcgaagtc gtccttggcc tcggagtcgg cgccggagat cgtcagaagg
     7081 ccctggcgcc actggtacca gaggtagtgc ttctggtgga tgtcgcccga gcggctgcgc
     7141 tcgaggtcgg actcgaggta gtcgtggagg accctgtggt tctcgtccc
//
LOCUS       DASSQV010000319         3970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1817848, whole genome shotgun sequence.
ACCESSION   DASSQV010000319 DASSQV010000000
VERSION     DASSQV010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817848"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2427
                     /locus_tag="VFL04_05565"
     CDS             <1..2427
                     /locus_tag="VFL04_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7839207.1"
                     /translation="SLPRGSETILLVEDEELIATVESHELRAAGYAVIRASTGEAAVA
                     LVAGRPGGIDLILMDINLGEGMDGTEAAREILKDNDIPVLFLSSHTERAIVEKTEEIS
                     SYGYVVKSSSFTVLEASIKMAFKLHGARRELAEQARRLRESEERYRLLHEQAGIGIAY
                     YSPEGRVISYNEEALRNMGASLDEVVGKGVEEMFPPEETEKYMGRLRRALSSPTKAEY
                     TDEVDLPTGRNWFESTYARICDAEGQPIGVQIMSKNITDRRRSELEEARRDEQLRLAL
                     HLARIGVWDWEIGSGRVSWSEEMHRIFGISPEDFAGPSGGTIDRTGEGFRAILAANMA
                     EAERRGITEEGLLVGGNIDPEPKELAIARPDGSRVSILGDAITIVSGGGEPLRMLGIT
                     QDISELRRAEDALRDSERRHAAVIENAPVLICEIASDGRYLSANPAYLQVLGYDPAEL
                     IGRAGLPAIHPDDAPRAFARLATLIETRGPSIETWRFRHRNGDYLWFECRASVYEDPK
                     GELVILVISADISERRRAEEEMRSLILQKETLMKELQHRVKNSLNVVSSLVELELGQV
                     RDEDSRAAFAKTMSRLSSIAGIYERLYLSEDLSSIDLEPYITELAGSIFETFNPGRIG
                     LRISVEHVSLDSRRAVPLGLILNELIINSLKYAYPGEASGELRIELAQSGGRLRLSVA
                     DDGVGWHGRAGAGSTPSLGMELVRMLAQQLRGDFSIEGERGTCARLSFDLSAGPEARG
                     PAPVGEGSAGSRPFFRRKSPRGPVQGLEFVLCSSGTQCLDQKVSARKRPKIPTVPDTA
                     QPARNRGYTY"
     gene            2467..2736
                     /locus_tag="VFL04_05570"
     CDS             2467..2736
                     /locus_tag="VFL04_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET7839208.1"
                     /translation="MGKKLYVGNLSYNTYEDSLRALFQTFGTVVSAKIITDRDSGSSK
                     GFGFVEMGTDDEARAAIQGANGRDLDGRQIKVNEAMDKPRRDDRY"
     gene            2875..3864
                     /locus_tag="VFL04_05575"
     CDS             2875..3864
                     /locus_tag="VFL04_05575"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7839209.1"
                     /translation="MLKRRLGRAGPEVSAIGLGCMGMSEFYGPSDDRASIGLIRRAVD
                     LGLDFLDTADMYGVGHNEMLLAQALRDGYRDRVVLATKFGNMRRIDGASLGVNGRPEY
                     VKAACDMSLRRLGTDHIDLYYQHRVDAEVPIEETVGAMAELVKAGKVRFLGLSEAGPA
                     TIRRANAVHPIAALQSEYSLWTRDPESGALATCRELGIGFVAYSPLGRGFLTGQIREP
                     SKLDARDFRKQQPRMVQANLEHNLGLVARIEAIAARKKVTAAQLALAWVLSRGQDVVP
                     IPGTRHEAYLRDNIAALELRLDREELEELDRALPPGAASGSRYNEEGMKRLGL"
BASE COUNT          739 a         1347 c         1313 g          571 t
ORIGIN      
        1 tctctgccac ggggaagtga aaccatactt cttgtcgagg acgaagagct gatcgcgacg
       61 gtcgaaagcc acgagctcag ggccgcgggc tacgccgtga tcagggcgtc aacgggcgag
      121 gctgccgtcg ccttggtcgc aggccgccct ggcggcatcg acctcatcct catggacatc
      181 aacctcggcg aggggatgga cggcaccgag gcggcccgcg agatcctcaa ggacaacgac
      241 attcccgtcc ttttcctctc ctcccacacc gagagggcca tcgtggagaa gaccgaggag
      301 atctcctcct acggctatgt ggtcaagagc tcctcattca ccgtactcga ggcctcgatc
      361 aagatggcct tcaagctcca cggcgcccgc cgagagctcg ccgagcaggc caggcggctc
      421 cgcgagagcg aggagcgcta ccgcctcctc catgaacagg ccgggatcgg catcgcctat
      481 tacagccccg aggggagggt catctcctac aacgaggagg cgctcaggaa catgggggcc
      541 agcctcgatg aggtcgtcgg caagggcgtc gaggagatgt tcccgcccga ggagaccgag
      601 aagtacatgg ggaggctcag acgggccctc tcctccccga ccaaggccga gtacaccgac
      661 gaggtcgatc tgccgacggg gaggaactgg ttcgagagca cctacgcgag gatctgcgac
      721 gccgagggcc agcccatcgg ggtccagatc atgtccaaga acatcaccga caggcggagg
      781 tccgagcttg aggaggcgag gagggacgag cagctgaggc tggccctcca cctggcgcgg
      841 ataggggtgt gggactggga gatcgggagc ggcagggtca gctggtccga ggagatgcac
      901 cggatcttcg gcatcagccc cgaggatttc gccggccctt ctggagggac gatcgacagg
      961 acgggcgagg gcttccgggc catcctggcc gccaacatgg ccgaggcgga gaggcggggc
     1021 atcaccgagg aggggctcct cgtcggcggc aacatcgacc ccgagcccaa ggagctggcc
     1081 atcgcgaggc ctgacggctc cagggtgagc atcctcggcg acgccatcac catcgtcagc
     1141 ggcgggggcg agcccctccg catgctcggc atcacccagg acatcagcga gctcaggcgg
     1201 gccgaggacg ccctccgcga cagcgagcgt cgccacgcgg ccgtcatcga gaacgccccc
     1261 gtcctgatct gcgagatcgc ctccgatggc cgctacctct cggccaatcc cgcctacctc
     1321 caggtcctgg gctacgatcc ggccgagctc atcggccggg ccggactgcc ggcgatccac
     1381 cccgacgacg ctccgagggc cttcgccagg ctcgccaccc tcatcgagac ccgtggcccc
     1441 tcgatcgaga cctggcgttt ccgccacagg aacggcgact acctctggtt cgagtgcagg
     1501 gcgagcgtct acgaggatcc gaagggcgag ctcgtgatcc tcgtgatctc ggccgacatc
     1561 agcgagcgca gacgcgccga ggaggagatg aggagcctta tcctccagaa ggagaccctc
     1621 atgaaggagc tccagcaccg cgtcaagaac agcctcaacg tcgtctcgag cctcgtcgag
     1681 ctggagctcg gccaggtcag ggacgaggat tcccgggcgg ccttcgccaa gaccatgtcg
     1741 aggctcagct cgatcgcggg gatctacgaa aggctctacc tctccgagga cctctcaagc
     1801 atcgacctcg agccctacat caccgagctg gcaggctcca tcttcgagac cttcaacccg
     1861 ggcaggatcg ggctcaggat cagcgttgag cacgtcagcc tcgactcgag gcgggccgtg
     1921 cccctcgggc tcatcctcaa tgagctcatc atcaacagcc tcaagtacgc ctatcccggg
     1981 gaggcgtcgg gggagctgcg catcgagctc gcccaatcgg ggggcaggct caggctctcg
     2041 gtggccgatg acggcgtggg ctggcatggg agggccggag cggggagcac gccgagcctg
     2101 ggcatggagc tcgtccgcat gctcgcccag cagctccggg gggatttctc gatcgagggc
     2161 gagcggggaa cctgcgcgag gctgagcttc gacctctccg cgggccccga ggcccgcggc
     2221 ccggccccgg tcggggaggg ctccgcaggc tcgcggcctt tttttcgccg aaagtcccct
     2281 cggggccctg tacaaggctt ggagttcgtg ctatgttcct cgggaacaca atgcctggac
     2341 cagaaagtat ctgcgcgaaa gcgacccaaa ataccaacag tcccagacac tgctcaaccc
     2401 gcgcgtaacc gcgggtacac gtattaagag acccgaagca cggacttcgg gcgaaggaac
     2461 tgatcaatgg gcaagaaact ctacgtcggc aacctcagct acaacaccta cgaggacagc
     2521 ctccgcgctc tcttccagac gttcggcacc gtggtctccg cgaagatcat caccgaccgc
     2581 gactctggca gctcgaaggg cttcggcttc gtggagatgg gcaccgacga tgaggcccgc
     2641 gccgccatcc agggcgccaa cggcagggac ctcgatggcc gccagatcaa ggtcaacgag
     2701 gcgatggaca agccccgccg cgacgaccgc tactaagctt tagcagccga aagccgaagg
     2761 agccgcccgc atgggcggct ctttttttgc ctcctggacc cccgccgggg cccggaccag
     2821 cagtttgcct tgggcgaaag cctcggtaga ttacaggaat aggcggaggt gcatatgctc
     2881 aagagaagac tcggccgcgc cggccccgag gtgtcggcca tcgggctcgg ctgcatgggc
     2941 atgtcggagt tctacggccc ttccgacgac agggcctcga tcggcctcat aaggagggcc
     3001 gtggacctgg gcctggactt cctcgacact gccgacatgt acggggtcgg gcacaacgag
     3061 atgctgctgg cccaggccct ccgcgacggc taccgcgaca gggtggtcct cgccacgaag
     3121 ttcggcaaca tgcgcaggat cgacggggcg tcgctcgggg tcaacggcag gcccgaatac
     3181 gtgaaggcgg cctgcgacat gagcctcagg cgcctcggca ccgaccacat cgacctgtac
     3241 tatcagcacc gcgtcgacgc cgaggtcccc atcgaggaga ccgtcggcgc gatggccgag
     3301 ctcgtcaagg cgggcaaggt gcgcttcctc ggcctttcgg aggccgggcc ggccacgatc
     3361 cgccgggcca acgccgtcca tccgatcgcc gccctccagt cggagtactc gctgtggacc
     3421 agggaccccg aatccggggc cctggcgacc tgccgcgagc tcgggatcgg cttcgtggcc
     3481 tacagccccc tcggccgggg tttcctgacc ggccagatcc gcgaaccctc caagctcgac
     3541 gcgcgggact tccgaaaaca gcagccgcgc atggtccagg ccaacctcga gcacaacctc
     3601 ggcctcgtcg cgcggatcga ggccatcgcc gcgcggaaga aggtgacggc cgcccagctc
     3661 gccctggcct gggtcctctc gcgcggccag gacgtggtcc ccatacccgg cacccgccac
     3721 gaggcctacc tgagggacaa catcgccgcc ctcgagctca ggctcgacag ggaggagctg
     3781 gaggagctcg accgggccct gccccccggc gcggcctcgg gctcccgcta caacgaggag
     3841 ggcatgaaga ggctgggcct ctagccggca cgggcccgga accggggatc gtcgatggtg
     3901 cgcaccagca ttaccaaccc gctctacatc gacgccgtgg agatagcctg gacggggggc
     3961 ctcgtcggca
//
LOCUS       DASSQV010000320         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1319566, whole genome shotgun sequence.
ACCESSION   DASSQV010000320 DASSQV010000000
VERSION     DASSQV010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319566"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1131)
                     /locus_tag="VFL04_05580"
     CDS             complement(256..1131)
                     /locus_tag="VFL04_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET7839210.1"
                     /translation="MRRVGIFYAFWTHEWDVDFLPFVAKVAGLGFEQLELNGGTIALM
                     EERERRAIANAARERGLALSYGIGLPAARDPSSLDETVRKEGVRFMRSMIDAVSSMGG
                     GMIGGTIHSTWPAKAPRDLAEKAAIRERSLLSMRELAPYAEDSGVVLNIEVLNRFEHF
                     LFNVADEAIQFVREVGSPALGILLDTFHMNIEEDSFGDAIRKTGPHLKALHLGETNRR
                     PPGQGRMPWKEIRAALDDIGFDGPLVMEPFLMPGGQVGRDIAVWHELSPGADDAALDA
                     MAAESAAFVKRTLRD"
     gene            complement(1128..>2155)
                     /locus_tag="VFL04_05585"
     CDS             complement(1128..>2155)
                     /locus_tag="VFL04_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET7839211.1"
                     /translation="AGYALATHYAHRMAGSVPASARRIGGICEFVAEGLVGAELGLSD
                     PSCLASYGAWEVSAGAFDAELLDELLGPGSPAYFAPAPPVSVAGLWMGRVPVAFPVGD
                     NQAGFHGLVADPLSACLVNLGTSGQLSFFSPEPKPAPGMELRPFLNRGYLQVGATLCG
                     GKAWEILEEFLRSAAELVTSHRVESEAVYAAMEALASSEAEGGEALRVLPSFGGTRAD
                     PSRRGAILGIGLDNLRPGNMVRALVAGVVEELRGFSGGLGGDFGRMERIVATGGFARR
                     VAAVPRALAEGFGLSVGLSGAEDGSAIGAALIGAEAAGLIDAAAVSRIVSSLPLSLVG
                     PRQRRGA"
BASE COUNT          322 a          760 c          766 g          307 t
ORIGIN      
        1 atcgagggac cggtcgggcc agtgcatgta cctgcctatg tccttgtcgg ccttgaaccc
       61 atacaacgtg tccaggtcct cttcgcggta gtcgcgcaat gaaaggcgct ccgtctcgag
      121 ctgcatacat cgcctcctgg cggccggttc ggataggctc ggcgtcagtc tcgagtgttc
      181 ggaagtcact cccgagcggc ggcgccacag tctggaccgt ctcgggaccc atggcaaggg
      241 tccgcgggcc cagccctagt cccgcaacgt ccgcttcaca aaggccgcgg actcggcggc
      301 catcgcgtcg agggcggcat cgtcggcgcc cggcgagagc tcgtgccaga cggcgatgtc
      361 ccggcccacc tggccgcccg gcatgaggaa gggttccatg acgagggggc catcgaagcc
      421 gatgtcgtcg agggcagccc tgatctcctt ccagggcatc ctgccctggc ctgggggcct
      481 gcggttagtc tcgccgaggt ggagggcctt gaggtggggg cctgtcttgc ggatcgcgtc
      541 gccgaaagag tcctcctcga tgttcatgtg gaaggtgtcg aggaggatgc cgagggccgg
      601 gctgcccacc tcgcgcacga actggatggc ctcgtcggcc acgttgaaga ggaagtgctc
      661 gaagcggttc aggacctcga tgttgaggac gaccccgctg tcctcggcgt agggggcgag
      721 ctcgcgcatg ctcaggaggc tgcgctcgcg gatcgccgcc ttctcggcga ggtccctcgg
      781 ggctttggcg ggccaggtcg agtggatcgt cccgccgatc atcccacccc ccatcgagga
      841 caccgcgtcg atcatcgacc tcatgaagcg gacgccctcc ttcctcaccg tctcgtccag
      901 ggacgatggg tcgcgcgccg cgggcaggcc gatcccgtag gagagggcga ggccccgctc
      961 ccgcgccgca ttggcgatgg cgcggcgctc ccgctcctcc atgagggcga tcgtgccccc
     1021 gttgagctcg agctgctcga agccgaggcc ggccaccttg gccacgaagg gcaggaagtc
     1081 gacgtcccac tcgtgggtcc agaaggcgta gaagatcccg actcgtctca tgctcccctc
     1141 ctttgccgcg gccccacgag gctcagcggc aggctcgaca cgatgcgcga gacggccgcg
     1201 gcgtcgatga gccccgcggc ctcggccccg atgagggcgg ccccgatggc cgagccgtcc
     1261 tcggccccgc tcaatcccac gctcaggccg aagccctcgg ccagggcgcg gggcacggca
     1321 gccacgcggc gggcgaagcc cccggtggcg acgatcctct ccatgcggcc gaagtccccc
     1381 ccgaggccgc ccgagaagcc gcgcagctcc tccaccacgc ccgcgacgag ggcccgcacc
     1441 atgttccccg gcctcaggtt gtcgaggccg atgccgagga tagcgccgcg ccgtgacggg
     1501 tcggccctcg tgccgccgaa gctgggcagg acccgcaggg cctcgcctcc ctcggcctcg
     1561 gacgaggcga gggcctccat ggcagcgtag acggcctcgc tctcgaccct gtggctcgtc
     1621 acgagctcgg cggccgagcg caggaactcc tcgaggatct cccaggcctt gcccccgcag
     1681 agcgtggccc ccacctggag atagccccgg tttaggaagg gccgcagctc catgcccggc
     1741 gcgggtttgg gctcggggct gaagaagctg agctggcccg aggtgccgag gttgacgaga
     1801 caggcggaca ggggatcggc gacgaggccg tggaagcccg cctggttgtc ccccaccggg
     1861 aaggccacgg gcaccctgcc catccagagc ccggcgaccg atacgggagg ggccggagcg
     1921 aagtaggctg ggcttcccgg gccgaggagc tcatcgagga gctcggcgtc gaaggccccg
     1981 gccgagacct cccaggcccc gtaggaggcg agacagcttg ggtccgaaag cccgagctct
     2041 gcgccgacga ggccctcggc cacgaactcg cagatcccgc cgatgcgccg ggccgaggcg
     2101 gggaccgagc ccgccatgcg gtgggcgtag tgggtggcga gggcgtagcc cgcgg
//
LOCUS       DASSQV010000321         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_325125, whole genome shotgun sequence.
ACCESSION   DASSQV010000321 DASSQV010000000
VERSION     DASSQV010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325125"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFL04_05590"
     CDS             <1..685
                     /locus_tag="VFL04_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839212.1"
                     /translation="RNRDYTAALLIAEESGSELHESQIDATLGKPFSYHRGDLAVFDL
                     DRCLIIDPAADYEDILLIVEHANYQLLELRVLDKLLDVWLDTAEKDIRRAYSGSGRTK
                     RILGSARLKFATIQALRFDSLFILENLENSSKIIGDYYLGQIYDRLCSIFNVEGWKWS
                     IERRLDALQGVYEMLKGESGERRMLTLEIIFIIVCIIPIVQFMLAENRAASEAQASPP
                     PAVSAPAKP"
     gene            736..>2809
                     /locus_tag="VFL04_05595"
     CDS             736..>2809
                     /locus_tag="VFL04_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7839213.1"
                     /translation="MTDRDELKAGERRVATILFSDMKGFTSLSEHADPEEMDGLMNRV
                     FGLFEEIIRGYGGQVEKYIGDALVAVFGVPDLHEDDPSRAVHSALDFQTRLKTESARV
                     QGRFAGRSGAAPNLSFRTGIHTGLVTTGRRGEFDVVTGHAMSVAQRIEAAAAPGTVFV
                     SEAVREKCEGEFEFDLPRRLEAKGKTEPVTIYQVKGEVSGAVRDSGPLVGRRELLDEM
                     LKAYIRNRYDEVSGFYLTGEAGSGKTRLIAAFLEKLRQFPDFATPILSARAQKFRPGG
                     FSVILDILLGYLGLEASVDRERAAKAAATLPGLSEAVVGRFTEIVCCADPGSPDSSSI
                     ATLYDVFESILERHAGDLYPIVVCIDNAASMDRLSREFFQYFFKHGRIKPFFLLAGRE
                     QPGELRKAFLGLKAMRIGPLAAEDSEALVRYHWPEAEGEGLERILSLSGGNPLFLREY
                     AAYARKHRDLSSLPATVQNIFLAGLERYPPDWRDLAKRLSSFLHSFTAADARYVQGAD
                     EAAAPWVEEALAQFEADGLLVREGDSYSFRLDVFKKALYSSLLNHNKRVLHGLIADIL
                     LKRERPHRIRLIHHLVRAERYAEAAKVLLDDPDRTYNYEALPYIDVLIRRLKDDGKAI
                     IRLLITKAAQLFNGGKIEESEAVLKRIMRTAIIRRDATLMGYAYHQICAYNHMTYSFQ
                     KAIFAGSKA"
BASE COUNT          467 a         1090 c          822 g          430 t
ORIGIN      
        1 gcgcaaccgc gactacaccg ccgccctcct catcgccgag gagtcgggct ccgagctcca
       61 cgagtcccag atcgacgcga cccttggcaa gcccttctcc taccaccgcg gcgacctggc
      121 cgtcttcgac ctcgaccgct gcctcatcat cgacccagcc gccgactatg aggacatcct
      181 cctcatcgtc gagcacgcca actaccagct cctcgagctc agggtcctgg acaagctcct
      241 cgacgtctgg ctggacacgg ccgagaagga catcaggcgg gcctattccg gcagcggccg
      301 cacgaagcgc atcctcggct cggcgaggct gaagttcgcg acgattcagg ccctccgctt
      361 cgactcgctc ttcatcctcg agaacctcga gaactcctcc aagatcatcg gagactacta
      421 cctcggccag atctacgaca ggctctgctc gatcttcaac gtcgagggct ggaaatggtc
      481 catcgagcgc cgcctcgacg ccctgcaggg ggtctacgag atgctcaagg gcgagtccgg
      541 cgagcgccgc atgctcaccc tcgagatcat cttcatcatc gtctgcatca tccccatcgt
      601 gcagttcatg ctcgcggaga accgcgcggc ctcggaggcg caggccagcc ccccgccagc
      661 ggtctcggcc ccagcgaaac cttgaggccg ccgcttgccc gggcggggac tgccccgtta
      721 cactctgcgt gaaccatgac cgaccgcgat gaactgaagg cgggcgagcg ccgcgtcgcc
      781 accatcctct tctcggacat gaagggcttc acgagcctct ccgagcacgc cgaccccgag
      841 gagatggacg gcctcatgaa ccgcgtcttc ggcctcttcg aggagatcat ccggggctac
      901 ggcggccagg tggaaaagta cataggcgac gccctggtgg ccgtcttcgg cgtgcccgac
      961 ctccacgagg acgacccctc ccgcgccgtc cactcggccc tcgacttcca gaccaggctc
     1021 aagaccgaga gcgcgcgggt ccagggccgc ttcgccggcc gctcgggcgc ggcgccgaac
     1081 ctctccttcc gcaccggcat ccacacgggg ctcgtgacca cggggcgccg gggcgagttc
     1141 gacgtggtca ccggccacgc gatgagcgtc gcccaacgca tcgaggccgc ggccgccccc
     1201 ggcacggtct tcgtctcgga ggcggtcagg gagaagtgcg agggcgagtt cgagttcgac
     1261 cttccccgcc ggctcgaggc caagggcaag accgagccgg tcaccatcta ccaggtcaag
     1321 ggcgaggtct ccggggcggt gcgggactcg ggccccctcg tgggccgccg ggagctcctc
     1381 gacgagatgc tcaaggccta catccgcaac cgctacgacg aggtctcggg cttctacctc
     1441 accggcgagg ccggctcggg caagacccgg ctcatcgccg ccttcctcga gaagctccgt
     1501 cagttccccg acttcgccac ccccatcctc tcggccaggg cccagaagtt caggccgggc
     1561 ggcttctcgg tcatcctcga catcctcctc ggctacctcg gcctcgaggc ctcggtggac
     1621 cgcgagcgcg cggccaaggc ggccgcgacc ctgcccggcc tgagcgaggc cgtcgtcggc
     1681 aggttcaccg agatcgtctg ctgcgccgac cccggcagcc ccgactcgag ctcgatcgcc
     1741 accctctacg acgtcttcga gtccatcctc gagcggcacg cgggcgacct ctatcccatc
     1801 gtcgtctgca tcgacaacgc ggcctcgatg gaccgcctgt cccgcgagtt cttccagtac
     1861 ttcttcaagc acgggcggat caagcccttc ttcctcctcg cgggccgcga gcagcccggg
     1921 gagctgcgca aggccttcct gggcctcaag gccatgcgga tcgggcccct cgcggccgag
     1981 gactccgagg ccctcgtgcg ctaccactgg cccgaggccg agggcgaggg cctcgagcgc
     2041 atcctgtccc tgagcggcgg caacccgctc ttcctcaggg agtacgccgc ctacgcgagg
     2101 aagcaccgcg acctctcctc cctccccgcg accgtgcaga acatcttcct cgcggggctc
     2161 gagcgctatc cccccgactg gcgcgacctc gcgaagcggc tctcgtcctt cctccactcc
     2221 ttcaccgccg ccgacgcccg ctacgtccag ggggcagatg aggccgcggc gccctgggtc
     2281 gaggaggccc tcgcccagtt cgaggccgac ggcctcctgg tcagggaagg cgacagctac
     2341 tccttccgcc tcgacgtctt caagaaggcc ctctactcga gcctcctcaa ccacaacaag
     2401 cgggtcctcc acggcctcat cgccgacatc ctcctcaagc gcgagcggcc ccaccgcatc
     2461 cgcctcatcc accacctcgt gagggccgag cgctacgccg aggcggccaa ggtcctcctc
     2521 gacgaccccg accgcaccta caactacgag gccctgccct acatcgacgt cctcatccgc
     2581 cggctcaagg acgacggcaa ggccatcatc cgcctcctca tcaccaaggc ggcccagctc
     2641 ttcaacggcg gcaagatcga ggagtccgag gccgtgctca agcgcatcat gaggacggcc
     2701 atcatccgga gggacgcgac cctcatgggc tacgcctacc accagatctg cgcctacaac
     2761 cacatgacct attccttcca gaaggcgatc ttcgccggct cgaaggccc
//
LOCUS       DASSQV010000322         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1818475, whole genome shotgun sequence.
ACCESSION   DASSQV010000322 DASSQV010000000
VERSION     DASSQV010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818475"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFL04_05600"
     CDS             <1..357
                     /locus_tag="VFL04_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET7839214.1"
                     /translation="WMPVVFAYPALFRIRAPLGQKYDFRYFTDREAESYELVNGRDYS
                     VVIPSKPFSLVDRVPFLRKEGIGKFILDFSWVELTKSLYKQVMKAAEEGRVLPDTGRF
                     NWKDGFWQAEETGPKD"
     gene            572..1393
                     /locus_tag="VFL04_05605"
     CDS             572..1393
                     /locus_tag="VFL04_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7839215.1"
                     /translation="MSESAGKALDLLFYLARTGGEITLARLSRDTGMNKATALRYLSV
                     LESRGVAERRGSGWSLGLALFELGARVPVRSIVVERVRPFLERLARETGETANLACLS
                     GGSAVYLDRAEADRSLRMRSVPGDRLPLYCTGVGKAILSLLPDERARAILGVGPLPKI
                     NEKTITDPEEVLKEAIKAREIGYGLDREEYETGLTCLAKPLRLPGSDFAGAISVSGPT
                     ARMRDPAIRDRFLDSLTRAVTDSQASLEALAAEADPQAILQAPKPAAAHHAVGGD"
     gene            complement(1390..1779)
                     /locus_tag="VFL04_05610"
     CDS             complement(1390..1779)
                     /locus_tag="VFL04_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET7839216.1"
                     /translation="MNSLNSILLEGNLVRDPESKTLPTGTEVCTFAIAADRFYKQAEG
                     ELENEVSYFDVEAWSKLGSACAQNLKKGRGVRVVGRLKQDRWTDQTGKTKSRVKIVAE
                     HVEFRPVKKEAAGAEGEKAGAEPAPVF"
     gene            1947..2624
                     /locus_tag="VFL04_05615"
     CDS             1947..2624
                     /locus_tag="VFL04_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839217.1"
                     /translation="MGSLIIAILVLGSLSVGAVVLAGYLSRSSQADRARLSPPEEEED
                     GLEQRLLSIEGKIRDLAMLRKVLGEVSSEVDVHSIVEKQDGILSYLSAYHDKYSGLVL
                     MRQVGERLKGLLSGELGVEAKVQGIVELKKEAQQRYLYLRERSVASAHDDLDRRKLEA
                     DRHLDAVLRDLVVVETNRIIASESPLTRIEGIERDEGAIFEAEGTESEAELNREYDRF
                     ISEIELA"
     gene            complement(2621..3298)
                     /locus_tag="VFL04_05620"
     CDS             complement(2621..3298)
                     /locus_tag="VFL04_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7839218.1"
                     /translation="MRKTLLCFGDSNTWGFDCERWVKDSPAFRFAPEVRWPGVLAAEL
                     GPDYAVIEEGHNGRTTVFDDEVEGEHRNALRYLQACLESHAPLDAVVIMLGTNDLKER
                     FAATASDIAAGAARLAQLVLASRVGPSAKAPAVLLVAPFPVGREIRSSPFGEMFGYEA
                     AIAKSRLFAERYAERAKALGLGFLDAGAVVTAHPKDSIHLGAAEHRKLGLAVAAALRP
                     MLQGARV"
BASE COUNT          515 a         1162 c         1124 g          526 t
ORIGIN      
        1 tggatgccgg tggtcttcgc ctacccggcc ctcttcagga tcagggcccc gctcggccag
       61 aagtacgatt tccgctactt caccgaccgc gaggccgaga gctacgagct cgtgaacggc
      121 agggactact cggtggtcat cccctccaaa cccttctctc tcgtcgatcg cgtccccttc
      181 ctgcgcaagg agggcatcgg caagttcatc ctggacttct cctgggtcga gctcaccaag
      241 tccctgtaca agcaggtgat gaaggccgcc gaggagggca gggtcctccc cgacacgggc
      301 aggttcaact ggaaggacgg cttctggcag gccgaggaga cgggccccaa ggactgaagc
      361 cggggccggc caggccggcc ccgcccctga gcggccccgc ccggggcggt tgcccggggt
      421 gggcccccat ttcacatggc ggaagtcacg attccctgat atgctgtttt gatgtaatcg
      481 tatacttgac gcggcctgaa cgggaatcta ctctatagtt cgataagcaa aacccaattt
      541 tgggaaatga accttctgca cggggagcag catgagcgaa tccgcgggaa aagccctcga
      601 cctccttttc tatctggcca ggacgggcgg cgaaatcacc ctcgctcggc tatcccgtga
      661 cacggggatg aacaaggcca cggccctccg ctacctctcg gtcctcgagt cgaggggggt
      721 cgccgagagg cggggctcgg gctggtccct cggcctggcc ctcttcgagc tcggcgcccg
      781 ggtgccggtg cgctccatcg tcgtggagcg ggtacggccc ttcctcgagc gcctggcgcg
      841 cgagaccggc gagaccgcca acctggcctg cctctcgggc gggagcgcgg tctacctgga
      901 ccgagccgag gccgacagga gcctccgcat gcgctcggtg cccggggaca ggctccccct
      961 ctactgcacc ggggtgggca aggccatcct ctcgcttctg ccggacgagc gggcgagggc
     1021 catcctcggc gtgggccccc tgcccaagat caacgagaag accatcaccg accccgagga
     1081 agtgctcaag gaggccatca aggcccgcga gatcggctac ggcctggacc gggaggagta
     1141 cgagaccggc ctcacctgtc tcgccaagcc cctgcgcctg cccggctccg atttcgccgg
     1201 ggcgatcagc gtctcgggcc cgacggcccg catgcgcgac ccggcgatcc gcgaccgctt
     1261 cctggactcg ctcacgcgcg cggtcaccga ctcccaggcc tcgctcgagg ccctggcggc
     1321 cgaggccgac ccccaggcca tcctccaggc ccccaagccg gccgcggccc accacgccgt
     1381 cgggggcgac tagaacaccg gcgccggctc ggcgcccgct ttctcgccct cggcccccgc
     1441 cgcctccttc ttcaccggcc tgaactccac gtgctcggcc acgatcttga ccctggactt
     1501 ggtcttgccc gtctggtcgg tccagcggtc ctgcttgagc ctgcccacga ccctgacgcc
     1561 ccggcccttc ttgaggttct gggcgcaggc tgacccgagc ttggaccagg cctcgacgtc
     1621 gaaataggag acctcattct cgagctcccc ctcggcctgt ttgtagaagc ggtcagccgc
     1681 gatcgcgaag gtgcagacct cggtccccgt cgggagggtc ttgctctcgg gatccctgac
     1741 caggttgccc tccagcagga tggaattgag cgagttcatc cccacgtcct cctcgcgcgg
     1801 tcttccgccg cgcactaggg atacgcggga acccgggcgc cccgcttgag ctgggcgcag
     1861 gctttgtcga ggccgcggca ttgtcgcagc cgcggcattg tctcggccgc ggcccggggt
     1921 tagagtaggg gtgtggggtt gacgccatgg gatccctgat catcgccatc ctcgtcctcg
     1981 gcagcctttc cgtgggcgcc gtcgtgctcg ccggctacct gagcaggtcc agccaggccg
     2041 accgggctcg gctgagcccc ccggaggagg aggaggacgg gctcgagcag cggctcctgt
     2101 ccatcgaggg caagatccgc gacctggcca tgctgaggaa ggtcctcggc gaggtctcct
     2161 ccgaggtcga cgtccactcg atcgtcgaga agcaggacgg gatcctctcc tacctcagcg
     2221 cctaccacga caagtactcg ggcctcgtcc tcatgcggca ggtgggtgag cggctgaagg
     2281 gcctgctctc gggcgagctg ggggtcgagg ccaaggtcca gggcatcgtc gagctcaaga
     2341 aggaggccca gcagcgctac ctctacctca gggagcgctc cgtcgcctcg gcccacgacg
     2401 acctcgaccg gcggaagctc gaggccgaca ggcacctcga cgccgtcctc cgcgacctcg
     2461 tcgtcgtgga gaccaaccgc atcatcgcga gcgagtcccc cctgacgagg atcgagggca
     2521 tcgagcgcga cgagggggcc atcttcgagg ccgagggcac ggagagcgag gccgagctca
     2581 accgcgagta cgaccgcttc atctccgaga tcgagctggc ctagacccgg gcgccctgga
     2641 gcatgggcct cagggcagcg gccaccgcga ggccgagctt ccggtgctcg gccgcgccga
     2701 ggtggatcga gtccttgggg tgggcggtga cgacggctcc cgcgtcgagg aagcccagac
     2761 cgagtgcctt cgcccgctcg gcgtagcgct cggcgaagag cctggacttc gcgatggccg
     2821 cctcgtagcc gaacatctcg ccgaagggcg aggagcggat ctcccgcccc acggggaagg
     2881 gggccacgag gaggacggcc ggggccttgg ccgagggccc gaccctgctc gcgaggacca
     2941 gctgggcgag cctcgcggcc cccgccgcga tgtccgaggc cgtggcggcg aaacgctcct
     3001 tgaggtcgtt ggtgccgagc atgatgacca cggcgtcgag gggggcatgg gactcgaggc
     3061 atgcctggag gtagcgcagg gcgttgcggt gctcgccctc gacctcgtcg tcgaagacgg
     3121 tcgtgcgtcc gttgtggccc tcctcgatca cggcatagtc cgggccgagc tcggcggcga
     3181 ggactccggg ccagcggact tccggggcga aacggaaagc cgggctgtcc ttgacccagc
     3241 gttcgcagtc gaagccccag gtgttcgagt cgccgaagca gagcagggtc tttctcatgg
     3301 tgcggcctcc gtgcccatca tggtcga
//
LOCUS       DASSQV010000323         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2067108, whole genome shotgun sequence.
ACCESSION   DASSQV010000323 DASSQV010000000
VERSION     DASSQV010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067108"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1258
                     /gene="rsmH"
                     /locus_tag="VFL04_05625"
     CDS             215..1258
                     /gene="rsmH"
                     /locus_tag="VFL04_05625"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET7839219.1"
                     /translation="MAEEATEPHRRRKRYKGSHPRAFAEKYKELDPERYPEDIEKVMA
                     RGDTPAGSHRSICVREVLEVLAPRPGELAADATLGHGGHALELLKAIQPGGRLYGLDR
                     DPLEIAKTEARLRAAGFDEDSFVARRMNFAQLPELLAAEGLAGLDMVLADLGVSSMQI
                     DNPERGFTFKRQGPLDMRMDPGSGQSAAELLASIPEARLRRIIEDYSDESEAPVIARV
                     LTQRRGTITTTRALAQAIRDALASRMNGEELNRKIIRRTFQALRIEVNGELDSLDRLL
                     AALPSCLRSGGRAAILCFHSGEEGRVELAFRQGREKGLYSSAAESGIRPGPEERYQNP
                     RSSSARLWWAVRA"
     gene            complement(1255..1785)
                     /locus_tag="VFL04_05630"
     CDS             complement(1255..1785)
                     /locus_tag="VFL04_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7839220.1"
                     /translation="MQGLESERLLLEDLVPGHAAELFPVLRDPEMYTYIPGDPPSSET
                     ELRERYTRQTNHAAEDELWLNWILRLKEGRECIGYVQATLPKASSALIAYQLSPRFWG
                     KGYAFEACGAVLARLATVHSVREIRAEIDSRNTASIRLVERLGFRREAFRERADFFKG
                     SWSDEIVFVLQLESRT"
     gene            2097..>2452
                     /locus_tag="VFL04_05635"
     CDS             2097..>2452
                     /locus_tag="VFL04_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839221.1"
                     /translation="MVYILVGLLGFLMVLASDGADLGRAHLLGLGLRIVGFLLVALAF
                     LGLGLTGSRLSLGPWLRIPGAALSLLFFGLFCSSFWTEVPQKAPTAAAGGHGLVTTGS
                     YALVRHPGLLWLLALHL"
BASE COUNT          395 a          830 c          839 g          388 t
ORIGIN      
        1 ggatgctcgg ccgcatttca cgggtgagat gggacattct gcgggttcgg ggcaggggag
       61 gcgggggcac gattttcctt gcgcgtccgg ggaaaccgtg ctagcgacta ggccgcgggg
      121 atgggccctt gccccttcgg ctccatcggg tcgaggagct gtcggaaggg gcctgaacca
      181 gcgtacaatg gccgcccgaa ggaaaggaca ggccatggca gaggaagcga cagaacccca
      241 ccgcagacga aaacgctaca agggcagcca cccccgggcc ttcgccgaga aatacaagga
      301 gctcgacccc gagcgctatc ccgaggacat cgagaaggtg atggcccgcg gggacacgcc
      361 ggcggggagc caccggagca tctgcgtgcg cgaggtcctc gaggtcctcg cccccaggcc
      421 gggcgagctc gccgccgacg cgaccctcgg ccacggcggc cacgccctcg agctcctaaa
      481 ggccatccag ccggggggca ggctctacgg cctcgaccgc gaccccctcg agatcgccaa
      541 gaccgaggcg aggctcaggg ccgcgggctt cgacgaggac agcttcgtgg ccagacggat
      601 gaacttcgcc cagctgcccg agctcctcgc ggccgagggc ctggccggcc tcgacatggt
      661 cctcgccgac ctcggcgtgt cctcgatgca gatcgacaac cccgagcgcg gcttcacctt
      721 caagcgccag ggccccctcg acatgaggat ggaccccggc tcggggcaga gcgcggccga
      781 gctcctcgcg tccatccccg aggcccggct cagacggatc atcgaggact actccgacga
      841 gagcgaggct cccgtgatcg cgcgtgtgct gacccagcgg cgggggacga tcacgacgac
      901 gagggccctc gcccaggcca tcagagacgc cctggccagc aggatgaacg gggaggagct
      961 caaccggaag atcatccgcc gcaccttcca ggccctgcgc atcgaggtga acggcgagct
     1021 tgactccctc gaccgcctcc tcgcggccct cccgtcctgt ctccgatccg ggggcagggc
     1081 cgccattctc tgtttccact cgggggagga gggcagggtg gagctggcct tcagacaggg
     1141 ccgcgaaaag ggcctctatt cctcggccgc cgagagcggg atcaggccag gtcccgagga
     1201 gaggtaccag aacccccgct cctcctcggc caggctctgg tgggcggtca gggcctaggt
     1261 ccggctctcg agctgcagga cgaagacgat ctcgtcgctc caggagccct tgaagaagtc
     1321 ggcccgctcg cggaaggcct ccctcctgaa gcccagccgc tccacgagac ggatcgaggc
     1381 agtgttcctt gagtcgatct cggccctgat ctcccgcact gagtggaccg tggcgagccg
     1441 ggcaaggacg gcgccgcagg cctcgaaggc atagcccttc ccccagaacc gcggggagag
     1501 ctggtaggcg atgagggccg aggaggcctt cgggagggtc gcctggacgt atcctatgca
     1561 ttcccggcct tccttgaggc gcaggatcca gttgagccag agctcatcct cggcggcgtg
     1621 gttcgtctgg cgggtgtagc gctcccgcag ctccgtctcg gaggaggggg gatccccggg
     1681 tatataggtg tacatctcgg ggtcccggag caccgggaag agctcggcgg cgtgtccggg
     1741 gaccagatcc tcaagaagga gcctctcgct ctccagaccc tgcatcacgc ctcccacggc
     1801 gccccgaggc ggcgcccaga tgggcgagcc gcggggcctc cgaaggggcg gggaatgccc
     1861 aaaggttatt atattgtaat gaccttgatt cctgcccccg cccgatatat gcttgagacg
     1921 ataacaggtc gcgccacaat ggtcagcccc ggctgtgcat ccggcatgtg cttctgtcta
     1981 tgaaaaatgt ctccggttgg gcacttcagg ggctacactt gaaccagggg ctgggggccg
     2041 gccggcggtt cgggactggc ccgtggcgtc gagggacatt cgggcaggag tggggaatgg
     2101 tgtacatcct cgtgggactt ctgggattcc tcatggtgct cgcctcggat ggagccgacc
     2161 tgggccgggc ccacctcctt ggcttgggcc tcaggatcgt gggcttcctc ctcgtggccc
     2221 ttgccttcct cggccttggc ctcaccggaa gccgcctctc cctgggcccg tggctccgga
     2281 tcccgggggc cgccctttcc ctcctgttct tcggcctctt ctgctcatcg ttctggaccg
     2341 aggtcccgca gaaggcgccg accgcggcgg ctgggggcca tgggctcgtc acgacgggga
     2401 gctacgccct cgtgaggcac cccgggctgt tgtggctcct cgccctccac ct
//
LOCUS       DASSQV010000324         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1070729, whole genome shotgun sequence.
ACCESSION   DASSQV010000324 DASSQV010000000
VERSION     DASSQV010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070729"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2453
                     /locus_tag="VFL04_05640"
     CDS             <1..>2453
                     /locus_tag="VFL04_05640"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET7839222.1"
                     /translation="LRSLVAERGAAFPDIRVATRTGDTSQAERRRMVSRPPSILVTTP
                     ESLCLILDSPKARQVLAELRLLILDEVHALSDSKRGSLLACAVGRLALLAGEFQRLAL
                     SATLRPPDAVAAFVGGRALSYRGEEAVYTPRRVSIVSPPAEKRIELSVEFPAAPVPDS
                     PGAPAGGFIAEAEDSRYAAVIPFMLARIRANRSTIVFCDSRRRAERIAFLLNEAGGEG
                     TAWAHHGSLSREARRVVEERMRAGELACVVATASLELGIDIGGVDEVILAGAPPAVSS
                     ALQRVGRSGHGVGETSRGLILPFYGMDLLLSAAASRAVDERAVEESRPLSCPLDILAQ
                     VLLSLSAEGPRRSDALYDIVRSFPPFETLPRGLFDSTLEMLAGRYSSARLRELESRVA
                     LDRASGVVTAGDGVRTLLYSSGGAIPDRGMYSLRVRGSRTRIGELDEEFVWERKVGDA
                     FSFGAQAWRIVEIGSEAVEVLPLGREADFMPFWKAGSRFRSPELAERSLALLDSLAPL
                     GPEASASLLEAEYHFAPEAARALVIFIGLQKAAGGSSPSLPGSRRIAIEANSDPARRG
                     DATRLVIHTLRGAAINETLALALAGAWEEEAGLPIQVIADDDSILALIPMLGGEGEAA
                     ATAARLLAALAAPGRLEALVRSRLEGTGLFGAQFRENAGRAILLPRGLPGKRTPLWIT
                     RLRAKKLFEAVRGFEDFPVTVETWRSCLGELLDLEGTRALVSAIALGDIEIGSFSTRS
                     PSPFSREAIWKETNEFLYRGDALEARAASSVSDRVVAEALRSSRLRPRLDPELVADFV
                     RRLKRLVPGWGPEDGFELA"
BASE COUNT          292 a          971 c          836 g          354 t
ORIGIN      
        1 ctgcgctccc tcgtcgccga gcgcggcgcc gccttccccg acatccgcgt cgcgacgagg
       61 acgggggaca cgagccaggc cgagcgccgc cgcatggtct cgaggccgcc ctcgatcctc
      121 gtgacgaccc ccgagagcct ctgcctcatc ctggactcgc ccaaggcccg ccaggtcctg
      181 gccgagctca ggctcctcat cctcgacgag gtccacgccc tctccgactc caagcggggc
      241 tccctcctcg cctgcgccgt gggccgtctg gccctcctcg cgggggagtt ccagcgtctg
      301 gccctctcgg ccaccctcag gccccccgac gccgtggccg ccttcgtcgg cggccgcgcc
      361 ctgtcctacc gcggcgagga ggccgtctac accccgcgca gggtctcgat cgtctcgccc
      421 cccgccgaga agcggatcga gctttcggtc gagttccccg ccgcgcccgt ccccgacagc
      481 cccggggccc cggctggcgg cttcatcgcc gaggccgagg actcccgcta cgccgccgtc
      541 atacccttca tgctcgcgcg gatccgcgcc aaccggagca ccatcgtctt ctgcgactcc
      601 cggcgccggg ccgagcgcat cgccttcctc ctcaacgagg cgggagggga gggcacggcc
      661 tgggcccacc acggctcgct ctcgcgcgag gcgaggcggg tggtcgagga gaggatgcgg
      721 gccggggagc tcgcctgcgt ggtcgcgacg gcctcgctcg agctcggcat cgacataggc
      781 ggggtcgacg aggtcatcct ggccggagcc ccacccgcgg tctcctcggc cctccagcgc
      841 gtcggccgct cgggccacgg ggtcggcgag acctcgcggg gcctcatcct gcccttctac
      901 ggcatggacc tcctcctgtc cgccgccgcg tcgagggccg tggacgagag ggcggtggag
      961 gagtcgcggc ccctctcctg ccccctggac atcctggccc aggtcctcct ctcgctctcg
     1021 gccgagggcc cgcgcaggtc ggacgcgttg tacgacatcg tcaggtcctt cccgcccttc
     1081 gagaccctgc cgcggggcct cttcgactcc accctcgaga tgctggccgg gcgctactcc
     1141 tcggcccggc tccgcgagct cgagagccgg gtggccctcg accgcgcgag cggcgtggtc
     1201 accgccgggg acggggtgcg gaccctgctc tactcctcgg gcggggccat ccccgaccgg
     1261 ggcatgtaca gcctccgcgt caggggctcg cggacccgca tcggcgagct cgacgaggag
     1321 ttcgtctggg agcgtaaggt cggggacgcc ttcagcttcg gggcccaggc ctggaggatc
     1381 gtcgagatcg ggagcgaggc cgtcgaggtc ctgcccctcg gccgcgaggc cgacttcatg
     1441 cccttctgga aggccggctc gcgtttccgg agccccgagc tcgccgagcg cagcctcgcc
     1501 ctcctggaca gcctggcccc cctcggcccc gaggcttccg cctccctcct cgaggccgaa
     1561 taccacttcg cgcccgaggc cgcccgagcc ctcgtcatct tcatcgggct ccagaaggcg
     1621 gccggcggga gcagcccctc ccttcccggc tcgaggagga tcgccatcga ggcgaactcc
     1681 gacccggcga ggcgggggga cgcgacccgg ctcgtgatcc acaccctccg gggcgccgcc
     1741 atcaacgaga ccctcgccct cgccctcgcc ggggcctggg aggaggaggc cgggctgccg
     1801 atccaggtca tcgccgacga cgattccatc ctcgccctga tcccgatgct gggcggggag
     1861 ggggaggccg cggcgaccgc ggcgaggctc ctcgccgccc tcgccgcccc ggggaggctc
     1921 gaggccctcg tgcgctcgag gctcgagggg acggggctct tcggcgccca gttccgcgag
     1981 aacgccggga gggccatcct cctgccccgc ggcctccccg gaaaacggac ccccctgtgg
     2041 atcacgaggc tcagggccaa gaagctcttc gaggcggtgc ggggcttcga ggacttcccc
     2101 gtcaccgtcg agacctggcg ctcctgcctc ggcgagctcc tcgacctcga ggggacaagg
     2161 gccctcgtgt cggccatcgc gttgggcgac atcgagatcg gctccttctc gacgaggagc
     2221 ccctcgccct tctcccgcga ggccatctgg aaggagacca acgagttcct ctaccgcggc
     2281 gacgccctcg aggcgagggc cgcctcctcg gtctcggacc gcgtggtggc cgaggccttg
     2341 cgctcctcga ggctcaggcc ccgcctcgat cccgagctcg tcgccgactt cgtgcggagg
     2401 ctcaagcgcc tcgtcccggg ctggggcccc gaggacggct tcgagctggc gga
//
LOCUS       DASSQV010000325         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_824759, whole genome shotgun sequence.
ACCESSION   DASSQV010000325 DASSQV010000000
VERSION     DASSQV010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824759"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1614
                     /locus_tag="VFL04_05645"
     CDS             142..1614
                     /locus_tag="VFL04_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET7839223.1"
                     /translation="MALFDMETRDIFENLSPLDHRYWNANRDIFEALSSCLSEKAQVR
                     YCARVEAALLATHLELRGRSRPELLASIERAAELIDPAAVYAEEETTRHNIRALVNVL
                     QRALPAELAPLVHLGATSADILDTAKALALGDAARTVVVPLLARVVLELCRLAVDEAD
                     TTQVGRTHGQHAVPLTFGFAMAEYASRLGKSVLRASSLARELRGKLSGAVGSYNALSM
                     SYREPEEVEASFLARLGLESSEHSTQIVEPEPVLRLLLELNVAFGVIANLADDLRNLQ
                     RTEIGEVRESFGSGQVGSSTMPQKRNPWNSEHVKSLWKAFSPRVATFFADQISEHQRD
                     LSNSASERFVPEYLAGLAAASNRMLGVLKGLVVDRERMKANLLMTGGSILAEPAYILL
                     AESGRPDAHEILRRITLAAEKGGLGFGAALAADKEAFEAIGRSLSAIGVDDARAFFAE
                     PGSYRGRAPEKARRIGEKYRALMTGLLEGRAAPAGGLAKA"
     gene            1733..>2840
                     /locus_tag="VFL04_05650"
     CDS             1733..>2840
                     /locus_tag="VFL04_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7839224.1"
                     /translation="MFEETYSYDDVLLVPGYSEVLPRETDISTRLAGEIRLNLPIVSA
                     AMDTVTEERMAVAIALQGGAGVIHRSLSPQAQADQAASVKRYLNWIIENPVTVEADRT
                     LRDVRAIMDRHRVTGLPVVDAEGRLVGIITARDLRFRRDEGALVREAMTAELVVEGLD
                     ASPASAREKFDEHRIEKLPVVDARGRLAGLITVKDMEKHERFPNAATDKAGRLIVGAA
                     VSPQDWELRMPLLKAAKVDFVVLDTAHGDSRNVIDAVRGIKRAYPDIPVVGGNVVTKE
                     GARRLIDAGSDAVKVGVGPGSICTTRVVAGVGVPQFSAVLWCAEECDRAGIPVIADGG
                     IKYSGDIVKAIAGGASAVMVGNLLAGLKEAPGREV"
BASE COUNT          457 a         1054 c          952 g          377 t
ORIGIN      
        1 aggaagaagc gctagccata cgactgcgcc gggccttgca ggtctgcgaa gcggacctgc
       61 cgcccacggc cttaaggcgt gggcgaggcc gtgggccgcg ccccccctcg gcgcccgcct
      121 cttgaaaagg gcggagccgc catggcatta ttcgacatgg agacccgcga catcttcgag
      181 aacctctctc ccctcgacca ccgatactgg aacgccaacc gggacatctt cgaagccctc
      241 tcctcatgcc tctccgagaa ggcccaggtc aggtattgcg caagggtcga ggctgccctc
      301 ctcgcgaccc acctcgagct ccgcggccgc tccagacccg agctcctcgc ctccatagag
      361 agggccgccg agctgatcga tccggccgcg gtctacgccg aggaggagac gacccgccac
      421 aacatcaggg ccctggtcaa cgtcctccag cgcgccctgc ccgccgagct cgcccccctc
      481 gtccacctcg gcgcgacgag cgccgacatc ctggacacgg cgaaggccct cgccctcggg
      541 gacgccgcca ggaccgtcgt cgtgcccctg ctcgcccgag tggtccttga gctctgcagg
      601 ctcgccgtgg acgaggccga caccacccag gtggggagga cccacggcca gcacgccgtc
      661 cccctgacct tcggcttcgc catggccgag tacgcgagcc ggctcggcaa gtcagtcctc
      721 agggcctcct ccctcgcccg cgagctccgg ggcaagctct ccggggcagt gggttcgtac
      781 aacgcgctgt ccatgagcta ccgcgagccc gaggaggtcg aggccagctt cctggcccgc
      841 ctcggcctcg agagctcgga gcactcgacc cagatcgtcg agcccgagcc cgtgctcagg
      901 ctcctcctgg agctgaacgt cgccttcggc gtcatcgcca acctcgccga cgacctccgc
      961 aacctccaga ggacggagat cggcgaggtg cgcgagtcct tcggctcggg ccaggtcggc
     1021 tcctcgacca tgccccagaa gcgcaacccc tggaacagcg agcacgtgaa gagcctctgg
     1081 aaggccttct cgccccgcgt cgccaccttc ttcgccgacc agatctcgga gcaccagagg
     1141 gacctctcga acagcgcgag cgagcgcttc gtccccgaat acctcgccgg gctcgcggcc
     1201 gcctccaacc gcatgctcgg ggtcctgaag ggcctggtcg tcgacaggga gcggatgaag
     1261 gccaacctcc tcatgaccgg aggctccatc ctcgccgagc ccgcctacat cctcctcgcc
     1321 gagtcgggca ggcccgacgc ccacgagatc ctgcgccgga tcaccctcgc ggcggagaag
     1381 ggcgggctcg gcttcggcgc agccctcgcc gccgacaagg aggccttcga ggccatcggc
     1441 cgctcgctct cggccatcgg ggtcgacgac gcgagggcct tcttcgccga gccgggcagc
     1501 taccgcggaa gggcgcccga gaaggcgcgc cgcatcggcg agaagtaccg cgccctcatg
     1561 acgggcctcc tcgaggggag ggccgcgccg gccgggggtc tggccaaggc ttagggaatc
     1621 aagggtccgc gcggactggc aagccggcgc gggccggctc cgcggggact tcggcataca
     1681 aggacggcca gcggctcccg cgagccgctc aaggcccaaa gagcggagca tcatgttcga
     1741 agagacctat tcctacgacg acgtcctcct ggtccccggc tactccgagg tccttccccg
     1801 agagaccgac atctcgaccc ggctcgccgg ggagatcagg ctcaacctgc ccatcgtctc
     1861 ggcggcgatg gacacggtca ccgaggagag gatggcggtg gccatcgccc tccagggcgg
     1921 ggcgggggtc atccaccgca gcctctcccc ccaggcccag gccgaccagg cggcctcggt
     1981 caagcgctac ctgaactgga tcatcgagaa ccccgtcacg gtcgaggccg acaggaccct
     2041 gcgcgatgtg cgcgcgatca tggacaggca ccgcgtcacc ggcctgcccg tcgtggacgc
     2101 cgagggacgc ctggtcggga tcatcaccgc ccgcgacctg cggttccgcc gggacgaggg
     2161 cgccctggtg cgcgaggcca tgacggccga gctggtggtc gagggcctgg acgcgagccc
     2221 ggcgagcgcc cgcgagaagt tcgacgagca ccgcatcgag aagctgccgg tcgtggacgc
     2281 caggggccgc ctcgctggcc tcatcacggt caaggacatg gagaagcacg agcgcttccc
     2341 gaacgcggcc accgacaagg cgggcaggct catcgtgggc gccgccgtat ccccccagga
     2401 ctgggagctc cgcatgcccc tgctcaaggc ggccaaggtc gacttcgtcg tcctggacac
     2461 ggcccacggc gattcgcgca acgtgatcga cgcggtgcgc ggcatcaagc gcgcctaccc
     2521 cgacatcccc gtggtcgggg gcaacgtggt gaccaaggag ggcgcgcgcc gcctcatcga
     2581 cgccggctcg gacgcggtca aggtcggggt ggggcccggc tcgatctgca ccacgagggt
     2641 ggtggcaggg gtcggcgtgc cccagttcag cgccgtgctc tggtgcgccg aggagtgcga
     2701 cagggccggg atccccgtca tcgccgacgg gggcatcaag tactcgggcg acatcgtgaa
     2761 ggccatcgcg ggcggggcct cggccgtcat ggtcggcaac ctcctcgccg gcctcaagga
     2821 ggcccccggg cgcgaggtac
//
LOCUS       DASSQV010000326         4316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2068387, whole genome shotgun sequence.
ACCESSION   DASSQV010000326 DASSQV010000000
VERSION     DASSQV010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068387"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VFL04_05655"
     CDS             <1..1039
                     /locus_tag="VFL04_05655"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HET7839225.1"
                     /translation="SSRAAKPAAAPGRAGGPAPAGLGSLLALAFPDRVAKRGEAKEDR
                     PRPGSARFRLPSGRTLGAEGSLAAWPWIVALDADAGMAEGRVYAGAGLTEADALAALA
                     PLITEELRIEWRGLGYRARRVRAALSIVLSETALGALPREEVARAFLERLRAEGLAIL
                     PLEGEAGAFLARLRWWRANSRSLGGAGLPDLTEEALAANAAEWLAPFIDPNPEAVLDA
                     RGLRRALETLLGHGLRSRFEAEAPETIELPSRARRRLEYAPDRDPAVEARIQELYGLS
                     VHPRILGRPVVFRLLSPAGRPVQVTADLPGFWRGAWTEVRKELRGRYPKHAWPEDPLH
                     AAPATGAPRRR"
     gene            1110..1337
                     /locus_tag="VFL04_05660"
     CDS             1110..1337
                     /locus_tag="VFL04_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET7839226.1"
                     /translation="MANKADRLKANVPGPWYVDSTCIACAVCTGDAPANFAMLDDGSS
                     AHVFKQPSGQELGQAETAMGDCPVQAIGNDG"
     gene            1398..2594
                     /locus_tag="VFL04_05665"
     CDS             1398..2594
                     /locus_tag="VFL04_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7839227.1"
                     /translation="MDRLLASLEAAGIGAYEASFTALDTYRGLRPAPLRFILAECGLV
                     DLAKNLEALRYPGLPFADAAIDSDEGDEESGGHSVSYIRCAESLHGAGRAALPPLDLL
                     YDPRRDVFHDPQGIYPSLRSATAELRPASPEDQLFQASILLSRQPYALAPGEPIPLPK
                     AVTVESQRSLLYLILTGTNPAAGLELLRTTGFLDAYWPELARLHGVDQSKEYHPEGDA
                     WSHTIETFAHRKLPSLRLSLALLLHDAGKPQSESAEGRRFDRHAEIGTRIAERFMARL
                     EFPRALVDDVSFLVRYHMLPAALPRLPMSRLEGIVDDPRFPILLELYKCDELSTFRGP
                     DGYYEACAAYRAYLKHRRNPWRADDGRKLARRYLEEAPRSGAPTRAGAAPRPAQASRR
                     RAPGPR"
     gene            2629..4008
                     /locus_tag="VFL04_05670"
     CDS             2629..4008
                     /locus_tag="VFL04_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HET7839228.1"
                     /translation="MFEASSYASRRSALIKALAARGVTSGLVLLVGNGESPMNYLDNT
                     YPFRQDSSFLYFIGISQAGLAATIDVSTGEACLFGDDLTIDDIVWTGPQPDMRQLASR
                     SGISRVRGRAELQAAVAGAAVHYFPPYRAETRAELASLLAKSPAAVDTGASLPLIRAT
                     IELRERKTAEELAELEIAVAVTVEMHRAAIATARPGMKESDVMARVAQVALSAGRLAF
                     PPIATTKGATLHIHSYDRRLESGGLFLLDAGAESPEGYSGDLTSTFPIDPKFDERQLA
                     IYEILLQTHALACGMIRPGLAYREVHFAAARSIVTGLKGLGIMKGDVDEAVASGAHAL
                     FFPHGLGHQMGLDVHDMENYGEVWVGYNGEPKSSQFGLRSLRLAKPLLTGMVLTVEPG
                     IYFIPELISLWKAEGRFTDFVNYDKLGPWMAVGGIRNEEDWLVTESGGRRLGPEFPKG
                     PKAIEEMRG"
BASE COUNT          635 a         1656 c         1404 g          621 t
ORIGIN      
        1 ctccagccgg gccgccaagc cagccgcggc gccgggaagg gcgggagggc cggcccccgc
       61 gggtctcggg agcctcctcg ccctagcctt ccccgaccgg gtggccaagc gcggcgaggc
      121 caaggaggac cgccccagac cgggttcggc ccggttccgc ctcccctcgg gcaggaccct
      181 cggcgccgag ggaagcctcg ccgcctggcc atggatcgtc gccctcgacg cagacgcagg
      241 catggccgag ggcagggtct acgcgggagc cggcctcacg gaggccgacg ccctggccgc
      301 cctcgcgccc ctcatcaccg aggagctgag gatcgagtgg cgcggcctgg gctaccgggc
      361 ccggagggtc cgcgccgccc tctcgatagt cctctcggag acggccctcg gtgccctccc
      421 gcgtgaggag gtggccaggg cctttctgga gcggctccgg gccgagggtc tggcgatcct
      481 ccccctggag ggggaggccg gggccttcct cgcccgtctg cgctggtgga gggcgaactc
      541 gcgctccctc ggcggagccg ggcttcccga cctcaccgag gaggccctcg cggcgaacgc
      601 ggcggagtgg ctcgcaccct ttatcgaccc gaaccccgag gctgtcctcg acgcccgggg
      661 cctccgtcgg gccctcgaaa ccctcctcgg ccatggcctg cgctcccggt tcgaggccga
      721 ggcccccgag accatcgagc tgcccagccg tgcccggcgg aggctcgagt acgcccccga
      781 ccgggaccca gcggtggagg cgaggatcca ggagctctac ggcctctcgg tccatcccag
      841 gatcctcggg cggccggtcg tgttcaggct cctctccccg gcgggcagac ccgtccaggt
      901 caccgccgac ctcccgggct tctggagggg ggcctggacc gaggtgcgca aggagctccg
      961 cgggagatac ccgaaacacg cctggcccga ggatcccctc cacgccgccc ccgccaccgg
     1021 agccccccgg cggcgctgag ctccgggagc cacgttgcgc gctggggaat gccgggtata
     1081 ctcagggtat cccctcgaag gaggccccta tggcgaacaa ggctgaccgc ctcaaggcaa
     1141 acgtccccgg tccgtggtat gtcgactcga cctgcatcgc ctgcgcggtg tgcacgggcg
     1201 acgctccggc gaacttcgcc atgctcgacg acggctcctc tgcccacgtg ttcaaacagc
     1261 cctcggggca ggagctcggc caggccgaga cggcgatggg ggactgtccc gtccaggcca
     1321 tcggcaacga cggctaggtc ccgcgggagg tccctcgggc gatttacctt ggggcccggc
     1381 ttcggtatca tcttcggatg gacaggctgc tggctagcct cgaggcggcg ggcatcgggg
     1441 cctacgaggc ctccttcacc gccctcgaca cctaccgcgg cctcaggccg gcgcccctaa
     1501 ggttcatcct cgccgagtgc ggcctcgtcg acctggccaa aaacctcgaa gcgctccgct
     1561 atcccggcct ccccttcgcc gacgccgcca tcgacagcga tgagggcgac gaggagtcgg
     1621 gcgggcactc ggtctcctac atcagatgcg ccgagtccct ccacggggcc ggcagggccg
     1681 cccttccccc cctcgacctc ctctatgacc cgcgcaggga cgtcttccac gacccccagg
     1741 ggatctatcc gagcctccgc tccgccacgg ccgagctcag gccggccagt cccgaggacc
     1801 agctcttcca ggcctccatc ctcctctcac gccagcccta cgccctggcc ccgggggagc
     1861 cgatccccct gcccaaggcc gtcaccgtcg agtcccagcg gagcctcctc tacctcatcc
     1921 tcaccggcac gaaccccgct gccggtctcg agctcctgcg cacgacgggc ttcctcgacg
     1981 cctactggcc cgagctggcc aggctccacg gggtcgacca gtccaaggaa taccaccccg
     2041 agggcgacgc ctggagccac acgatcgaga ccttcgccca ccgcaagctg ccctccctcc
     2101 gtctttccct ggccctcctc ctccacgacg ccggcaagcc ccagtccgag tcggccgagg
     2161 ggaggaggtt cgaccgccac gccgagatcg gcacgaggat cgccgagcgc ttcatggcga
     2221 ggctcgagtt cccccgagcc ctggtcgatg acgtgtcctt cctcgtccgc taccacatgc
     2281 ttcccgccgc cctgccccgg ctcccgatga gccgcctcga gggcatcgtc gacgacccgc
     2341 gcttccccat cctcctcgag ctgtataagt gcgacgagct ctccaccttc cggggccccg
     2401 acggctacta cgaggcctgc gccgcctatc gcgcctacct caagcacagg cggaacccct
     2461 ggagggccga cgacgggagg aagctcgcca ggcgctatct cgaggaggcg ccgcggtcgg
     2521 gcgctccgac ccgggcgggc gccgcgcccc gtcccgcaca ggccagccga cggagggccc
     2581 cgggcccacg ttgacgcggc cccgaggcga ggctagcatg ctgccaccat gtttgaagcc
     2641 agcagctacg cctccagacg ctccgccctc atcaaggccc tcgcggcccg tggtgtgacc
     2701 tcaggtcttg tcctcctcgt gggcaacggc gagagcccca tgaactacct cgacaacacc
     2761 taccccttcc gccaggactc gagtttcctc tattttatag ggatctccca ggccggtctt
     2821 gcggcgacga tcgacgtgtc gacgggggag gcctgcctct tcggcgacga cctcacgatc
     2881 gacgacatcg tgtggaccgg cccccagccc gacatgcggc agctcgcctc gcgctcgggg
     2941 atctccaggg tccgcggccg cgccgagctc caggccgccg tcgccggcgc cgccgtccac
     3001 tacttccccc cctaccgcgc cgagacgagg gccgagctgg cctccctcct ggccaagagc
     3061 cccgcggccg tggacacggg cgcctcgttg cccctcatca gggcgacgat cgagctccgg
     3121 gagcgcaaga cggccgagga gctggccgag ctcgagatag cggtcgcggt gacggtggag
     3181 atgcaccgag cggccatcgc cacggcgagg ccgggcatga aggagtcgga cgtgatggcc
     3241 cgcgtggccc aggtcgccct ctccgccggc cgcctcgcct tcccgcccat cgcgaccacc
     3301 aagggcgcca ccctccacat ccactcctac gacaggcggc tcgagtcggg tgggctcttc
     3361 ctcctcgacg ccggggccga atcgcccgag ggctattccg gcgacctcac ctcgaccttc
     3421 ccgatagacc cgaagttcga cgagcgccag ctcgccatct acgagatcct cctccagacc
     3481 cacgccctcg cctgcggcat gatcaggccg ggtctggcct accgggaggt ccattttgcc
     3541 gccgcccgga gcatcgtcac cgggctcaag ggcctcggga tcatgaaggg cgacgtcgac
     3601 gaggcggtcg cctcaggcgc ccacgccctg ttcttccccc acggcctcgg ccaccagatg
     3661 gggctcgacg tccacgacat ggagaactac ggcgaggtct gggtgggcta caacggcgag
     3721 ccgaagagca gccagttcgg tctgcgctcc ctcaggctcg cgaagcccct gttgaccggg
     3781 atggtcctca ccgtcgagcc cggcatctac ttcatccccg agctcatctc gctgtggaag
     3841 gccgaggggc ggttcacgga cttcgtgaac tacgacaagc ttggcccctg gatggccgtc
     3901 ggcgggatca ggaacgagga ggactggctg gtcaccgagt cgggcggcag gcggctcggg
     3961 cccgagttcc cgaagggccc caaggccatc gaggagatgc gcggctagcc ttcgatcgcc
     4021 cggccgcgcg gagtccccgt tcccagacca ccgggctccc ttgggcgaaa tgcattacat
     4081 tggtggtagg cgcctcgccg tttcgccacc agtggcgggc ggcgccggaa gggagaccag
     4141 catggccgaa gtcttcgcga gcatgttcca ggacgaggcc cttgtcgtga agagcctcat
     4201 cgagtcggcg ggcatcgagg ccgagatcgc gggcgagcac gtcgttgacg tctaccccgc
     4261 cttcttcccc gaaagcgggg gcatggtcat cagcgtgccc gacgaggacg gcgagg
//
LOCUS       DASSQV010000327         3581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1694208, whole genome shotgun sequence.
ACCESSION   DASSQV010000327 DASSQV010000000
VERSION     DASSQV010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694208"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..90)
                     /locus_tag="VFL04_05675"
     tRNA            complement(19..90)
                     /locus_tag="VFL04_05675"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Glu,seq:ctc)
     gene            complement(171..241)
                     /locus_tag="VFL04_05680"
     tRNA            complement(171..241)
                     /locus_tag="VFL04_05680"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(207..209),aa:Gln,seq:ctg)
     gene            complement(286..675)
                     /locus_tag="VFL04_05685"
     CDS             complement(286..675)
                     /locus_tag="VFL04_05685"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HET7839229.1"
                     /translation="MRKARLFGAALACLLASACVSSEVYAVRAMRIGHPVLSSLPDGR
                     RPGTFSYGGFTYEVAVTISGGRMAAIDILKNRGTKPARMAEGVIPRILEAQSPDVDAV
                     SGATTTSKALMKAVENALASPSDGKTP"
     gene            complement(672..2807)
                     /gene="recJ"
                     /locus_tag="VFL04_05690"
     CDS             complement(672..2807)
                     /gene="recJ"
                     /locus_tag="VFL04_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET7839230.1"
                     /translation="MKWTKKELPPSLVKELAARFDTDALTASILARRSVTAPEDVLYY
                     LEDDPRYLHNPFLFSQMEDAVDRILLAADEGEKVLVFGDRDADGVTSTVLMAEALAEL
                     GIEASWRVPTGSEPYGLSVQAVEAFAAEGGSLIVTVDCGISNHAEVERAAELHVDVVI
                     IDHHLLQAGEAPAAIAIIDPKLPDSGYPFRDLAGCGVVYKLICALKLARSGVYKQQVA
                     LLNVRPANEAFIVEAVRLSNLVETGRIRETIVPGVVDLERTRLVPFLRDRQIFVWDGE
                     SQRRLLARALGKGAEVNFYDVAPEVGEHIPQARGMSLLRLKELSRIGRYRSEPLEEID
                     VLENLFVSFALKKADWFGEDDMARLQLVALGTIADLMPLRNENRILVRRGLAAMNERA
                     RSGLAELLGKLGHAGKRLGAVDIAWQVTPVINAAGRMGEPQTAVSLFIAKERAEREAS
                     AEKVIQHNLERRRLGGEDWELVYPLARESLAEHGDKLALVGSASVHRGITGLIASRLT
                     STLKTPAIVASFQDDGTVVGSIRSARGFPVASFLDSCSDLFIDYGGHDEAAGFSLKAE
                     DWPRFQERARLFTVPLELEDGEELIEIDAELPHQFLKPEIAELTERFEPYGEESGPLV
                     FLARDVAILDAQIMGKKEQNHLKLTLDFGKHKWPALFWDGAQRLERDFSFKNHDRIDL
                     VFKVTLNRYNGMEQPQLEAFDARRTEKTT"
     gene            complement(2804..>3581)
                     /locus_tag="VFL04_05695"
     CDS             complement(2804..>3581)
                     /locus_tag="VFL04_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET7839231.1"
                     /translation="LITASILSKKVAEGADALIFDVKSGPGAFMKTLAEAEALAESLV
                     RTGKAMGKRIVGVITDMSEPLGFKVGNFLEVEESLDCLEGHGPADIMELSYRLGAWML
                     IAAGLEPDIKAGTQACERAIASGKAKELFLANVRSQGGDVDAMLALRGRHRAPAVLEL
                     KAPAVGFVAGIDAYKIGLAGVNLGVGRNKTTDPVFPDVGFVFERKTGDRVAAGDRICS
                     VYARDQAALEAARPLVESALSYSPSKPERRPLVIKEISAL"
BASE COUNT          531 a         1126 c         1314 g          610 t
ORIGIN      
        1 caggatctgc ggacctttta gcccctaggg gagtcgaacc cctctttaaa gattgagaat
       61 cttttgtcct aaccgataga cgaaggggcc atcatgatcg agccggcctc gcgaaaagcc
      121 ggctcgatca tggcgcggaa ggggccttcg cctcttccgc gcacgtcttg ttccccaggg
      181 aggatttgaa cccccacaag cagatccaga gtctgccgtg ctaccattac acaaccgggg
      241 atcgtcaatc agcacgcgta ccttatcgaa aaccgccccg tttcgtcaag gggttttccc
      301 gtccgaaggg gaggccaggg cgttctccac cgccttcatg agggccttgc tcgtggtggt
      361 cgcgccggac acggcgtcga cgtcggggct ctgggcctcg aggatgcggg ggatgacgcc
      421 ctcggccatc ctcgccggct tggtgccccg gttcttgagg atgtcgatgg ccgccatccg
      481 gccccccgag atcgtcacgg ccacctcgta ggtgaagccg ccgtaggaga aggtcccggg
      541 ccgtctgcca tcgggaaggg aggacagcac ggggtggccg atcctcatgg ccctcacggc
      601 gtagacctcg ctcgacacgc aggccgatgc gaggaggcag gccagggcgg cgccgaacag
      661 ccgggctttt ctcatgtcgt cttctccgtt ctgcgggcgt cgaaggcctc gagctggggc
      721 tgctccatgc cgttgtagcg gttgagggtg accttgaaga cgaggtcgat gcggtcgtgg
      781 ttcttgaacg agaagtcgcg ctcgaggcgc tgggccccgt cccagaagag ggccggccac
      841 ttgtgcttgc cgaagtccag ggtgagcttg aggtggttct gctccttctt ccccatgatc
      901 tgggcgtcga ggatggcgac gtcgcgggcg aggaagacga gggggcccga ctcctcgccg
      961 tagggctcga agcgctcggt cagctcggcg atttcgggct tgaggaactg gtgggggagc
     1021 tcggcgtcta tctcgatgag ctcctcgccg tcctcgagct cgagggggac ggtgaagagc
     1081 cgggcccgct cctggaagcg gggccagtcc tcggccttga ggctgaagcc cgccgcctcg
     1141 tcgtggccgc cgtagtcgat gaagaggtcg gagcaggaat cgaggaagga ggcgacgggg
     1201 aagccccggg cggagcggat cgagccgacc acggtgccgt cgtcctggaa cgaggcgacg
     1261 atggcggggg tcttgagggt cgaggtgagc ctcgaggcga tgaggccggt gatgccgcgg
     1321 tggacgctcg cgctccccac cagggccagc ttgtccccgt gctcggcgag gctctcgcgg
     1381 gccaggggat agaccagctc ccagtcctcg cccccgaggc ggcggcgctc gaggttgtgc
     1441 tggatcacct tctcggcgct ggcctcgcgt tcggccctct ccttcgcgat gaagagcgag
     1501 acggcggtct ggggctcgcc catgcggccc gcggcgttga tgacgggggt gacctgccag
     1561 gctatgtcga ctgccccgag cctcttgccc gcgtggccga gcttgccgag gagctcggcg
     1621 aggccggacc tggctcgttc gttcatcgcg gcgaggcccc gcctcacgag gatgcggttc
     1681 tcgttccgca ggggcatgag gtcggctatc gtgccgaggg ccacgagctg gagccgggcc
     1741 atgtcgtcct cgccgaacca gtcggccttc ttgagggcga aggagacgaa gaggttctcg
     1801 aggacgtcga tctcctccag gggctcggag cggtagcggc cgatgcgcga gagctccttg
     1861 agcctgagca ggctcatgcc cctggcctgg gggatgtgct cgccgacctc gggggccacg
     1921 tcgtagaagt tgacctcggc ccccttgccg agggccctcg cgaggaggcg cctctgggac
     1981 tcgccgtccc agacgaagat ctggcggtcg cggaggaagg ggaccagccg cgtgcgctcg
     2041 aggtcgacca cgcccgggac gatggtctcc ctgatcctcc cggtctccac gaggttcgag
     2101 agcctcacgg cctcgacgat gaaggcctcg ttggccggcc tgacgttgag gagggcgacc
     2161 tgctgtttgt agaccccgga gcgggcgagc ttgagggcgc agatgagctt gtagacgacc
     2221 ccgcagccgg cgaggtcgcg gaaggggtag cccgagtcgg ggagcttggg gtcgatgatg
     2281 gcgatggccg cgggcgcctc gcccgcctgg aggaggtggt ggtcgatgat gacgacgtcg
     2341 acgtggagct cggcggcccg ctcgacctcg gcgtgattcg agatgccgca gtccacggtg
     2401 acgatgaggc tcccgccctc ggcggcgaag gcctcgaccg cctggacgct gagcccgtat
     2461 ggctccgagc cagtgggcac cctccagctc gcctcgatcc cgagctcggc cagggcctcg
     2521 gccatgagga cggtgctcgt gaccccgtcg gcgtcgcggt ccccgaagac gaggaccttc
     2581 tcgccctcgt cggcggcgag gaggatgcgg tccacggcgt cctccatctg gctgaagagg
     2641 aaggggttgt ggaggtagcg cgggtcgtcc tcgagatagt agaggacgtc ctcgggggcg
     2701 gtgaccgagc ggcgggcgag gatcgacgct gtcagcgcgt cggtgtcgaa acgcgccgcg
     2761 agctccttga cgagggaggg ggggagctcc ttcttggtcc acttcatagg gcgctgattt
     2821 cctttatgac gagggggcgg cgttcgggct tggaggggga gtagctgagg gccgactcga
     2881 cgaggggcct ggccgcctcg agcgcggcct ggtcgcgcgc gtacacggag cagatcctgt
     2941 cccctgccgc cacccggtcc cccgtcttcc tctcgaagac gaagccgacg tcggggaaga
     3001 cggggtcagt ggtcttgttc ctgcccacgc caaggttgac ccccgcgagg ccgatcttgt
     3061 aggcgtcgat gccggcgacg aagcccaccg cgggggcctt gagctcgagg acggcggggg
     3121 cccggtgtct gcccctgagg gcgagcatcg cgtcgacgtc cccgccctgg ctcctcacgt
     3181 tggcgaggaa gagctccttc gccttgccgg aggcgatggc ccgctcgcag gcctgagtcc
     3241 ccgccttgat gtcgggctcg agcccggccg cgatgagcat ccaggcgccg aggcggtagc
     3301 tgagctccat gatgtcggcg gggccgtggc cctcgagaca gtcgagcgac tcctcgacct
     3361 cgaggaagtt gcccaccttg aagccgaggg gctcgctcat gtcggtgatg acgccgacga
     3421 tgcgtttgcc catggccttg ccggtgcgga cgaggctctc ggcgagggcc tcggcctcgg
     3481 cgagggtctt catgaaggcc cccgggcccg acttcacgtc gaagatgagg gcatcggccc
     3541 cctcggcgac cttcttggag aggatcgaag cggtgatcag c
//
LOCUS       DASSQV010000328         3690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1322253, whole genome shotgun sequence.
ACCESSION   DASSQV010000328 DASSQV010000000
VERSION     DASSQV010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322253"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFL04_05700"
     CDS             <1..578
                     /locus_tag="VFL04_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7839232.1"
                     /translation="KGMEWLAKQMGYKGNLLLITGIPSPIDTIRTDTIKTVVAKYPNI
                     KLLDVQPGDWNRQKSTTVMENYLQKYKQVDAVYTADDDMMLGAMQAYKESGRSDIKFW
                     LGGGCLKDVIKGIMDGKPMMAGYPVVMADVTYSPSVIATSISYAVIGVKGDKLNPYTY
                     QVRALPRRVILPSELVTKENAKDFYVPAAPF"
     gene            797..1879
                     /locus_tag="VFL04_05705"
     CDS             797..1879
                     /locus_tag="VFL04_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7839233.1"
                     /translation="MALKIGIVGAGGMARYHIAGFRSAGAEVLALADPSKAARERSAA
                     AFAVAETYDGLDAMLAARKDLDAVSIITPNKFHAPVAIQALKAGKHVYCEKPPALNAA
                     EVEAMAAAAKAAGRHLQFGFNNRARPETRALMDYVRAGEVGRINSAQAVWARRSGIPG
                     FGGWFTTKALSGGGPVIDLLHMLDLALYFMDFPEPAYVLAQTFRDFMEDRRFKGPWGI
                     PDVAGGATDVESACHGFVSFKGGQVLYLRSSWAEMTERELVSMSFQGAKAGGLVERSF
                     GRDGLDETSADRCELYTVEHGAQVNRRILVPFDETMGRHGAAANFVAAVEGREAPLNT
                     PEQAVRLMRIVDAIYASAAKAEPVKP"
     gene            1916..2908
                     /locus_tag="VFL04_05710"
     CDS             1916..2908
                     /locus_tag="VFL04_05710"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7839234.1"
                     /translation="MKALLLLIPMLLLGCQSLRFRDPVKPASEIARDPAGLDARVLGG
                     MEKYGVEKTSIALIARNKIVYEQGYNVDPGELLQAASISKVLTAYAALQLVEQGKLEL
                     DRPLDAYLKESYFPDGSAGRRITLRMVLTHTCGMGNDIRGKDRTIYHEPGKSFHYSGA
                     GFIFLMTVMEELVGMPFDQYMESRVMKPLGMEHSRFSIDYPSGFRSVSAASSLRSSAG
                     ELALFFEELLSPRFLSSGLAKAMLSDAVRIDGHYSWGLGIGIQHAEGSTAIWHWGNNA
                     DVYHSLALCFLEEGTGIVVMTKGKQGPLLYQDLAHYALGGSYFGLGSTVIRKTR"
     gene            complement(2949..3314)
                     /locus_tag="VFL04_05715"
     CDS             complement(2949..3314)
                     /locus_tag="VFL04_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7839235.1"
                     /translation="MYAIDSFVLYVEDIERSLAFYALITGSQPARLSPTFVAVEFGEG
                     PRLELKQRAESVPPATVTGGGTELSIRVPDEAALRALYGEWKGLGVRILQEPTSMVFG
                     TTFVGLDPDGHRLRVFAGG"
     gene            complement(3399..>3690)
                     /locus_tag="VFL04_05720"
     CDS             complement(3399..>3690)
                     /locus_tag="VFL04_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET7839236.1"
                     /translation="VIAIRRAIEAELVVHLAYTDAESRRTERDIWPFSLAYYDQSLVV
                     MAWCEQRSDFRNFRVDRIAALELDSRRYPEGRAELLRRWQEKERIPLDRYEL"
BASE COUNT          656 a         1259 c         1181 g          594 t
ORIGIN      
        1 gcaagggcat ggagtggctg gcgaagcaga tgggctacaa gggcaacctg ctcctgatca
       61 ccggcatccc ctcgcccatc gacaccatcc gcaccgacac catcaagacc gtcgtggcga
      121 agtatcccaa catcaagctc ctcgacgtcc agcccggcga ctggaaccgc cagaagtcga
      181 cgaccgtcat ggagaactac ctgcagaagt acaagcaggt cgacgccgtc tacaccgccg
      241 acgacgacat gatgctcggc gccatgcagg cctacaagga gtcgggccgc tccgacatca
      301 agttctggct cggcggcggc tgcctcaagg acgtgatcaa gggcatcatg gacggcaagc
      361 ccatgatggc tggctacccg gtcgtcatgg ccgacgtgac ctactccccc tcggtcatcg
      421 ccacctcgat ctcctacgcc gtgatcggcg tcaagggcga caagctcaac ccctacacct
      481 accaggtcag ggcccttccc cgccgcgtca tcctcccctc ggagctcgtg acgaaggaga
      541 acgccaagga cttctacgtc cccgccgcgc ccttctgacg gtggcctgct gaagagtagt
      601 tgaagaggcc gcagaggcgc agaggcgcag agggacgata ggagggaagt ttgggaaggg
      661 ggatcggaga gcctcagtca aactggggca tcctgtgcgc tctcctactt ccatcttctc
      721 ttctccctct tggctcagcg tctctgcgcc tctgcggttg atttcttctg gtagtctatt
      781 tccaaggagc gcgcgtatgg ccctcaagat aggcatcgtc ggggcgggcg ggatggcccg
      841 ctaccacatc gccggcttcc gctcggcggg ggccgaggtc ctggccctgg ccgacccgag
      901 caaggcggcg cgggagcgca gcgccgccgc ctttgcggtg gccgagacct acgatggcct
      961 cgacgcgatg ctcgcggcga ggaaggacct cgacgccgtc tccatcatca cccccaacaa
     1021 gttccacgcc cccgtggcca tccaggccct caaggctggc aagcacgtgt actgcgagaa
     1081 gccccccgcc ctcaacgcgg ccgaggtgga ggcgatggcc gccgccgcca aggccgcggg
     1141 caggcacctg cagttcggct tcaacaaccg agcccggccc gagacccgcg ccctcatgga
     1201 ctacgtccgt gccggcgagg tgggcaggat caactcggcc caggccgtct gggcccggcg
     1261 ctcgggcatc cccggcttcg ggggctggtt caccactaag gccctctcgg gcggtggccc
     1321 ggtgatcgac ctcctccaca tgctcgacct cgccctctac ttcatggact tccccgagcc
     1381 agcctacgtc ctcgcccaga ccttccgcga cttcatggag gacaggcgct tcaagggccc
     1441 ctggggcatc cccgacgtcg cgggcggggc caccgacgtc gagtcggcct gccacggctt
     1501 cgtgagcttc aagggcggcc aggtcctcta cctccgctca tcctgggccg agatgaccga
     1561 gcgcgagctc gtgtccatga gcttccaggg cgcgaaggcc ggaggcctcg tggagcggag
     1621 cttcggccgg gacggcctcg acgagacgag cgccgaccgc tgcgagctct acaccgtcga
     1681 gcacggcgcc caggtgaacc gcaggatcct cgtccccttc gacgagacca tgggcaggca
     1741 cggcgccgcc gccaacttcg tcgccgccgt cgagggcagg gaggcccccc tgaacacgcc
     1801 cgaacaggcg gtcaggctca tgaggatcgt cgacgccatc tacgcctcgg cggccaaagc
     1861 ggagccggta aagccctagg gccaagacgg cttctttgag cccgaaggag cgaggatgaa
     1921 agccctgctg cttctgatcc ccatgctgct tctggggtgc cagagcctga ggttcaggga
     1981 tcctgtgaag ccggcctcgg agatagcccg ggaccccgca ggtctggacg cgcgggtcct
     2041 gggagggatg gagaagtacg gggtcgagaa gacatcgatc gccctgatcg ctcggaacaa
     2101 aatcgtctac gagcagggct acaacgtgga cccgggcgag ctcctccagg ccgcctcaat
     2161 aagcaaggtc ctgaccgcct atgccgccct gcagctggtg gagcagggca agctcgagct
     2221 ggacaggccg ctcgacgcct atctcaagga gagctatttc cccgatggct cggccggacg
     2281 aaggatcaca ctgcggatgg tgctgaccca cacctgcggc atgggcaatg acatcagggg
     2341 caaggaccgg acgatctacc atgagccggg gaagagcttc cactattccg gagccggctt
     2401 catcttcctc atgacggtga tggaggagct ggtcggcatg cccttcgacc agtatatgga
     2461 gagccgggtg atgaagcccc tcggcatgga gcacagcagg ttcagcatcg actacccgag
     2521 cggcttcagg agcgtctcgg ctgcctcgag cctcaggagc agcgcgggcg agctggccct
     2581 gttcttcgag gagctcctct cgcccaggtt cctgagcagc gggctcgcga aggcgatgct
     2641 gtccgacgcg gtgaggatag acgggcacta ctcatggggc ctgggcatcg gcatccagca
     2701 cgccgagggc agcacggcca tctggcactg gggcaacaac gccgacgtct accattccct
     2761 ggccctctgc ttcctcgagg aggggacggg gatcgtggtc atgaccaagg gaaagcaggg
     2821 cccgctcctg taccaggacc tggcgcacta cgctctcggc gggtcctact tcggcctggg
     2881 gagcacggtg attcggaaga cccgctgatg cgcggcacgc ccctcctccc gcaccggctc
     2941 gccccggttc agcctccggc gaagacccgc aggcggtgcc cgtcgggatc gaggcccacg
     3001 aaggtcgtgc cgaagaccat gctcgtcggc tcctgcagta tcctcacccc gaggcccttc
     3061 cattcgccgt agagggcgcg cagcgcggcc tcgtcgggca ccctgattga gagctcggtc
     3121 ccgccccccg tcaccgtggc gggcggcacg gattccgcgc gctgcttcag ctccagcctc
     3181 ggcccctcgc cgaactcgac ggcaacgaag gtcggggaca gcctggcggg ctggctgccc
     3241 gtgatcagcg cgtaaaaggc caggctccgc tctatgtctt cgacatagag gacaaaggaa
     3301 tcgatagcgt acatgggctc tcctcgccgc acctttcttg gcgcggtcat cgccccgaat
     3361 ctaccagagc cccgtgtcag aaactgtcag cagagttttt agagctcgta gcggtcgagg
     3421 gggatcctct ccttctcctg ccagcgccgc aggagctcgg ccctgccctc cggatagcgc
     3481 ctcgaatcca gctccagggc ggcgatcctg tccacgcgga aattcctgaa gtcgctgcgc
     3541 tgttcgcacc aggccatcac gacaagggac tggtcgtagt aggccaggga gaagggccag
     3601 atgtcccgct ccgtccttcg gctttcggca tcggtgtagg cgaggtggac gacgagctcg
     3661 gcctcgatcg cccggcggat cgcgatgacg
//
LOCUS       DASSQV010000329         5793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2442181, whole genome shotgun sequence.
ACCESSION   DASSQV010000329 DASSQV010000000
VERSION     DASSQV010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2442181"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFL04_05725"
     CDS             complement(<1..519)
                     /locus_tag="VFL04_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HET7839237.1"
                     /translation="MQYQAFHVDAFTDRIFAGNPACVVPVEAWPEDALCLSIAAENAV
                     AETAFFRREGDGFRLRWFTPDIEMDLCGHATLAAAHVIMEELGLTRNLVRFSSASGIL
                     EVSREAGLYWLDFPARPPAPARLPAEIAESLNIAPIGVYKARDYLLEYASEEEVLGLA
                     PDRALMDRINLDP"
     gene            complement(526..1188)
                     /locus_tag="VFL04_05730"
     CDS             complement(526..1188)
                     /locus_tag="VFL04_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839238.1"
                     /translation="MKKTMLIAPFGGALAAAIAAEARAAGWAVALAMPGPPRAGQEKT
                     EDDPGGDTATLSYNPSSYVSVAALVLSARNALGEIDAAVLVADRGSGGLDLVASKPGD
                     IAALVEEKCAGPLYLARELVRRFEARRSGSILMLSSEPPRDASSGPSTSLVDSAFEGL
                     GRGLFGLSSGASWRAYGVQDASSQADRAARFALGLLGEAKGAKAGRWIRFTGRTGIFG
                     GA"
     gene            1242..2990
                     /locus_tag="VFL04_05735"
     CDS             1242..2990
                     /locus_tag="VFL04_05735"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET7839239.1"
                     /translation="MLVALAQMNAVIGDFDGNRERIVAFAEEAVGRGGGRIARRSEAG
                     IGAQGRPELLVFPELALCGYPPMDLLDQDSFVEANLGSLRRLQRELPRGIAVAVGLVE
                     RNRSGQGRPLVNEVAVILDGEIIFTQAKTLLPTYDVFDEARYFEPARERRVLSLGGRR
                     IGFAICEDIWWEEPPVPGLRYPVDPVKDLLDGGADVIIVPSSSPFHAGKLELRMRLSA
                     KAAREGGVPFLYCNLLGANDSLVFDGRSFVLDAEGRSRIVSGWEEELVFAELDELGPG
                     SAATLAPAASQEAKTERWDEVERALVLGIRDYMRKCGFKKACVGLSGGIDSALVALLG
                     AEAVGPENVTCISMPSRFSSKGSIDDSVELCRNLGMRLESLPIEGPFTAYLDLLEPKF
                     EGRPHDLTEENLQARIRGSLLMAWSNKFGSLLLTTGNKSELATGYCTLYGDMSGALAP
                     IGDLFKTEVFALCRAINARASGGGSRKAVIPEAILDKPPSAELRANQTDQDSLPPYSL
                     LDEVLELYIIRDLGAAEIVARGYESALVERVLAMVARSEYKRRQSAPVIKVSPRAFGT
                     GRRIPIARVVHESSPR"
     gene            3132..5249
                     /locus_tag="VFL04_05740"
     CDS             3132..5249
                     /locus_tag="VFL04_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839240.1"
                     /translation="MRPQAMGLVALACLALAATMSCQASSGLLSDSERKSLYTISITA
                     NGSAVADGSVLDAASALQVAVTKASEAEEPQSLELSLENPEPSSRITARMDVAPLAAG
                     PPPQGAVPAEGGGQAGTAEPAASAAPADGSEPTAAAGTAQSGEPPSAPASSEAAAPAK
                     AQGSAPEPLLHVGRITGTMPPVSIGTKLASGAYRLSASIFGADRALLQRSSFVVFVGT
                     ANPAIGSVSVYPPVAEPGEALLLQIELELPTPAGGARAPDPWIRWTREGRAFAEGPLS
                     KGFDRVVWTAPWVDGAYALLVEAFPSAPPDGEGFGFRSRVRQEFKAMVKASGGGEDEF
                     ADATLFSSLLRFEGNLDDAGRRARSAPPSALGTPRLDVYPGGFGYRFGATAGVNLPGL
                     MPPSSEGLMSDFSILFRLSADGGDGRLVRFASEDGGFALTLGLRQHRPFVEILADGKT
                     SRSVADSAIPQDPMTLVASFARDGGKLRIVWHAEGVRVKSPSLNVPQVPQRGGASIGG
                     PDSLEGVYDAFGVMQSGAPPAFRMASRRAWKTGLVLAEGFEDGRLPPGAQTTGGAAAR
                     AGRVELPAGSSIRFPQALDSSAQLHLEAEFSGEPRRAALLLEPAGTNQAFVVLGTGEV
                     LDDSGKRLGALSSSGGSLAFEIGPSPDRTEVLLSQAGSTVRLPLRPGASLGLSVGRDE
                     GSGPLALERIIVRGSSAPVPARP"
     gene            5385..>5793
                     /locus_tag="VFL04_05745"
     CDS             5385..>5793
                     /locus_tag="VFL04_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839241.1"
                     /translation="MRNVLRSAVSLAVMVLLASCASSPAPAPAAPAAAAPNAAQSRAQ
                     AIVQGTATPTPAPAQAQAPAAAPAAAPVSADQPPPAPGQLSPDEQAYLQNYLGRLNYM
                     VYYNADAKIDPKLAKIAVSQANRYLIEKMGLSVI"
BASE COUNT          871 a         2092 c         1950 g          880 t
ORIGIN      
        1 ggggtcgagg tttatgcggt ccatcagggc gcggtccggg gcgaggccga ggacctcctc
       61 ctcggaggcg tactccagca ggtaatccct cgccttgtac accccgatcg gagcgatgtt
      121 gagcgactcc gcgatctcgg cgggcagccg ggccggcgca ggaggcctcg ccgggaagtc
      181 cagccagtag agcccagcct cgcggctcac ctcgaggatc ccgctcgccg agctgaagcg
      241 cacaaggttc ctcgtcagcc cgagctcctc catgatcacg tgggcggcgg ccagggtcgc
      301 gtggccgcag aggtccatct cgatgtcggg cgtgaaccag cggagccgga agccatcgcc
      361 ctcgcggcgg aagaaggcgg tctcggccac ggcgttctcg gcggcgatgc tgaggcagag
      421 ggcatcctcg ggccaggcct ccacggggac gacacaggct ggattgccgg cgaagatgcg
      481 gtcggtgaag gcgtcgacat ggaaggcctg gtactgcatg gggcttcagg ctcctccgaa
      541 gatgcccgtc ctgccggtga aacggatcca gcggccggcc ttggcgccct tcgcctcgcc
      601 caagaggccg agggcgaagc gggcggccct gtcggcctgg gagctcgcgt cctggacccc
      661 gtaggcccgc catgaggccc ccgaggaaag accgaagagg ccgcggccca gaccctcgaa
      721 ggccgaatcg acaagcgagg tcgaggggcc gctcgaggcg tcccgcggcg gctccgagga
      781 gagcatgagg atgctgcccg acctgcgcgc ctcgaagcgc ctgaccagct cgcgcgccag
      841 atagaggggg cctgcgcact tctcctcgac gagagcggcg atgtcccccg gcttcgacgc
      901 gacgaggtcg aggccgcccg agccgcggtc ggccacgagg acggcggcgt cgatctcgcc
      961 cagggcattg cgggcgctga gcacgagggc tgctaccgag acgtaggatg aggggttata
     1021 ggagagggtg gcagtgtcgc cgcccgggtc gtcctcggtc ttctcctggc ccgctcgggg
     1081 cgggcctggc atcgcgagag cgaccgccca accggcagcc cgcgcctccg cggcgatggc
     1141 cgcggcgagc gcgcctccga agggggcgat cagcattgtc ttcttcacgc ggccgagtgt
     1201 actatgagca tacacacact gcaaggctcc ggaggttccg catgctcgtc gctctcgccc
     1261 agatgaacgc cgtcataggt gacttcgacg gcaaccgcga gaggatagtc gcctttgccg
     1321 aggaggccgt cggaagaggc gggggcagga tcgcgcggag gagcgaggcc gggatcggcg
     1381 cccaaggcag gcccgagctc ctcgtcttcc ccgagctcgc cctctgcggc tatccgccga
     1441 tggacctgct cgaccaggac tccttcgtcg aggcgaacct cggctccctc cggcgtctgc
     1501 agcgcgagct tccccgcggc atcgccgtcg ctgtcggcct cgtcgagcgc aaccgctccg
     1561 gccagggccg gccgctcgtg aacgaggtcg ccgtcatcct cgacggcgag atcatcttca
     1621 cccaggccaa gaccctgctc ccgacctatg acgtcttcga cgaggcgagg tacttcgagc
     1681 cggccaggga gcgccgcgtc ctctccctcg gcgggcggag gatcggcttc gcgatctgcg
     1741 aggacatctg gtgggaggag ccgcccgtcc ccggcctccg ctatcccgtg gacccggtca
     1801 aggacctcct cgacgggggg gccgacgtga tcatcgtccc ctcgtcctcc cccttccacg
     1861 cgggcaagct cgagctcagg atgaggctgt cggccaaggc cgcgcgcgag gggggcgtgc
     1921 ccttcctcta ctgcaacctc ctcggggcca acgactcgct cgtgttcgac ggccgctcct
     1981 tcgtgctcga cgccgagggc cggagtcgca tcgtctccgg ctgggaggag gagcttgtct
     2041 tcgccgagct cgatgagctg ggtccggggt cggcagcgac gctcgcaccg gccgcttcgc
     2101 aggaggccaa gaccgagcga tgggacgagg tcgagcgcgc cctcgtgctg gggatcaggg
     2161 actacatgcg caagtgcggc ttcaagaagg cctgcgtggg cctctcgggc gggatcgatt
     2221 cggcccttgt ggccctgctg ggcgccgagg cggtcggacc cgagaacgtc acctgcatct
     2281 ccatgcccag ccgcttctcg agcaagggct cgatcgacga ctcggtggag ctctgccgga
     2341 acctcggcat gaggctcgaa agcctcccca tcgagggccc cttcaccgcc tacctcgacc
     2401 tcctcgagcc caagttcgag ggccggcccc atgacctgac cgaggagaac ctccaggcca
     2461 ggatccgcgg ctccctcctc atggcctggt cgaacaagtt cggctcactt ctcctcacga
     2521 ccggcaacaa gagcgagctc gccacgggct actgcactct gtacggcgac atgtcgggag
     2581 ccctggcgcc gatcggcgac cttttcaaga ccgaggtctt cgccctctgc agggccatca
     2641 acgcccgcgc gagcggcggc ggaagccgca aggccgtgat ccccgaggcc atcctcgaca
     2701 agccgccgag cgccgagctt cgggcgaacc agaccgacca ggactccctg ccgccctact
     2761 ccctcctgga cgaggtcctc gagctctaca tcatacggga cctcggcgcg gccgagatcg
     2821 tcgcccgggg ctacgagagc gccctggtcg agagggtcct cgccatggtg gcgcgctccg
     2881 agtacaagag gcggcagtcg gcgcccgtga tcaaggtgtc gccccgggcc ttcgggacgg
     2941 ggcggcgcat accgatagcc cgggtggtcc acgagtcctc gccccgctag cgtctcggcg
     3001 gcccgccaga gcccaggacg agggcgggct tccattcctc tttcgggact tcctcgcctt
     3061 catggtttgg cggggccccg aaaccgatgt atactgtgcc caatgagggt gccgtgcccc
     3121 gagggtttgt tgtgagacct caagctatgg gcctcgtggc cttggcctgc ctcgccttgg
     3181 cggccacgat gtcctgccag gcctcgtcgg gcctcctctc cgactccgag cgcaaatccc
     3241 tatatacgat atccatcact gcgaacggca gcgccgtcgc cgacggcagc gtcctcgacg
     3301 cggcctctgc cctccaggtc gccgtcacca aggcgagcga ggccgaggag ccccagtccc
     3361 tcgagctgag cctggagaat cccgagccca gctcgcggat cacggctcgg atggacgtgg
     3421 cccccctcgc ggccggccct ccccctcagg gcgcagtccc ggccgaggga ggcggacagg
     3481 ctggtaccgc cgagcccgcg gcctcggcgg caccggcaga tggctcggag ccgacggcag
     3541 cagcggggac ggctcaatcc ggcgagccgc cttcggcccc ggcatcatcc gaggcggcag
     3601 ccccggccaa ggcccagggc tcggccccag agcccttgct ccacgtgggg aggatcaccg
     3661 gcaccatgcc gcccgtctcc atcggcacga agctcgcgag cggcgcatac cggctctccg
     3721 cgagcatctt cggcgccgac agggccctcc tccagcgctc gagcttcgtc gtcttcgtgg
     3781 gtacggcaaa ccccgccatc ggctcggtgt cggtctaccc gcccgtggcc gagcccggcg
     3841 aggccctcct gctacagatc gagctcgagc ttccaacacc tgcgggcggc gctcgggccc
     3901 ccgatccatg gatccgctgg acgagggagg gccgcgcctt cgccgagggg cccctgtcga
     3961 agggcttcga ccgcgtcgtc tggacggctc cctgggtcga cggagcctat gccctcctcg
     4021 ttgaggcctt cccctcagct ccccccgacg gggagggatt cggcttccga tcgcgggtca
     4081 ggcaggagtt caaggccatg gtcaaggcct cgggcggcgg ggaggatgag ttcgcggacg
     4141 ccaccctgtt ctcctccctg ctcaggttcg agggcaacct cgacgacgcc ggaaggaggg
     4201 cgcgcagcgc gcccccatcg gccctcggca cgccgcgcct cgatgtgtac cccggcggtt
     4261 tcggctatcg cttcggcgcg accgcggggg tgaacctgcc gggcctcatg cccccgagct
     4321 ccgagggcct catgtccgac ttctcgatcc tgttccgcct ttccgccgac gggggcgacg
     4381 gcaggctcgt tcgcttcgcg tccgaggacg gaggctttgc cctcaccctc gggctccgcc
     4441 agcaccgtcc cttcgtcgag atcctcgccg atggcaagac ctcgcgctcg gtcgcggact
     4501 ccgccatccc ccaggatccc atgaccctcg tggcgtcctt cgcgagggat ggcggcaagc
     4561 tccggatcgt atggcacgcc gagggcgtcc gcgtgaaatc tccgtcgctc aacgtccccc
     4621 aggtcccgca gaggggaggg gcctcgatag gaggacctga ttcccttgag ggcgtctacg
     4681 acgcctttgg ggtaatgcag agcggcgcgc cgccggcctt caggatggcc tccaggcggg
     4741 cctggaagac gggcctcgtc ctggccgagg gcttcgagga cggaaggctc cccccgggag
     4801 cccagaccac gggaggggcg gccgcgaggg cgggcagggt tgagcttcct gcaggatcga
     4861 gcatccgctt cccgcaggcc ctggacagct ctgcgcagct ccatctcgag gccgagttct
     4921 cgggagagcc gcgccgcgcc gccctcctcc tcgagcctgc gggcacgaac caggccttcg
     4981 tggtcctggg cacgggcgag gtccttgacg attccggcaa gaggctcggc gcgctcagct
     5041 ccagcggggg aagcctggcc ttcgagatcg gaccctcgcc cgacaggacc gaggtcctcc
     5101 tctcccaggc cggctcgacc gtgaggctcc ccctccgccc gggcgccagc ctgggcctct
     5161 cggtcgggag ggacgagggc tccgggcccc tggccctcga acggatcatc gtgcggggga
     5221 gcagcgcccc tgtccccgcg cggccctgat cttgcatggg cccagcccag ccactagact
     5281 gagtctgaca tcaacagccg cgatccacag gaaatgcgcc cggagtgcat tggtttttcc
     5341 ggggccctgc gttagattat ttgcagtaca acaaggagag atgcatgaga aatgtgctca
     5401 ggtccgcggt ctcgctcgcg gtcatggtcc ttctggcgtc ctgcgcttcg tcgcctgccc
     5461 cggctccggc cgcccccgca gcagcagcac ccaacgccgc ccagtcccgc gcccaggcga
     5521 tagtccaggg cacggcgaca ccgactcccg ccccggcaca ggcacaggct cccgcggccg
     5581 cccctgccgc agccccggtc tcggcagacc agcccccgcc cgctcccgga cagctgagcc
     5641 ccgacgaaca ggcctacctc cagaactacc tcggccgcct caattatatg gtgtactata
     5701 acgccgacgc gaagatcgat cccaagctcg cgaagatcgc agtctcgcag gcgaaccgct
     5761 atctgataga gaagatgggt ctctccgtga tcg
//
LOCUS       DASSQV010000330         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1571041, whole genome shotgun sequence.
ACCESSION   DASSQV010000330 DASSQV010000000
VERSION     DASSQV010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571041"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFL04_05750"
     CDS             complement(<1..257)
                     /locus_tag="VFL04_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839242.1"
                     /translation="MPEASGEAARAPGPGSVRAFGAPASAGSEGLSRPMASAAAGAGT
                     ALMSISASAVAGVSGGGATILVRVSSGRTSGPRLGSRSSGAA"
     gene            420..710
                     /locus_tag="VFL04_05755"
     CDS             420..710
                     /locus_tag="VFL04_05755"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7839243.1"
                     /translation="MPETKPGLAPKDVVLVPALSKGSFYIQVGVFGSSEAIQKAAGGF
                     ASGYPLAMERVEMKTGSAYRLYVGPLSRDESGVVLYRIKSLGFKDAFLRVGT"
     gene            complement(791..2263)
                     /locus_tag="VFL04_05760"
     CDS             complement(791..2263)
                     /locus_tag="VFL04_05760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET7839244.1"
                     /translation="MPPVLGLLALCLNAGCAATGGRIELSAGPGLRARLAEILEASPL
                     PKAWVPTEADGEATLSLVATPADPAQQPGAHETGGPLSADAGLLWLAPALPISSPLYR
                     LDSAQARAAGLEALDEILPPRRAASVDGRWPGQPGYPFVERLELRLSSRRRIPPVLAS
                     WLAGAAAAAKAEPAPIVLGAAGDLQVGPRSGLSLIGGEAGLERVFAGGLLPLMRSRDL
                     LVANLEGPITARGSPNPRKRFHFRFPPGSAKALGDAGFSLLLFANNHGFDFGEEGFLD
                     TLEDLEGAAMPFVGAGRNEEEAGQPRVLAALPGGQRLSFVGFASYPTERLGFTTAEAL
                     AGAAKPGIAGDPRSTLDAIRIAAAAGDTVIVLAHGGREYVESPTAETRELYRGFADAG
                     ARLVIGSHPHVLQGLEARGSSLIAYSLGNFLFTEELEPPQAVGSALLSFLLYRGIPRG
                     FLPEPVSAGIDGSRLEHGEAARLGVERLLSRLSSGLEAGP"
     gene            complement(2326..>2539)
                     /locus_tag="VFL04_05765"
     CDS             complement(2326..>2539)
                     /locus_tag="VFL04_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839245.1"
                     /translation="SLFYGASGAPDRDRSVTIHEALLTAGQILGSIAGGVVYQLVSWS
                     WVFIFLALLMAAGLALQLGMVRTRRG"
BASE COUNT          383 a          877 c          945 g          334 t
ORIGIN      
        1 gctgcccctg agcttcttga gcccagcctg gggccggacg tcctgcccga ggataccctg
       61 acgaggatgg tcgctcctcc cccggagacc ccggccacgg cgctggccga gatcgacatg
      121 agcgccgtgc ctgcgcccgc cgcggccgag gccataggcc tggacaggcc ttccgagccc
      181 gcgctcgccg gagcgccgaa cgccctgacc gagccgggcc ccggggccct cgcggcctcc
      241 cccgaagcct cgggcatcga gaagcctgcc cagaccggga ctcctcccgc gatagccctc
      301 gccgagccct cgacgagcca gcccgagcct ccccagtccg cgccagctcc ggcggctccc
      361 ggcgagctgt cggtcgccct tgagcctgcg gccccgcggc ggcggccaag cccgcggctt
      421 tgcccgagac gaagcccggc ctcgccccga aggacgtggt cctcgtgccg gccctctcca
      481 agggctcctt ctacatccag gtcggggtct tcggctcgag cgaggccatc cagaaggccg
      541 ccgggggatt cgcctcgggc tatcccctcg cgatggagag ggtcgagatg aagaccggtt
      601 cggcctatcg cctctacgtc ggtccgctct cgcgcgacga gagcggggtg gtcctctaca
      661 ggatcaagtc cctcggcttc aaggacgctt tcctcagggt cggaacctga gcatcaggca
      721 gcatgatgag caaggccgcc ccgccccggg gcggcctttt tttgacgccg gccttgtcgg
      781 tgacctcgcc tcatggcccc gcttcaagcc cggaggaaag ccgcgagagg aggcgctcca
      841 cgccgaggcg cgccgcctcg ccatgctcga gtcggctgcc atcgatcccc gctgacacgg
      901 gctcgggcag gaagccgcgg gggatccccc ggtagagcag gaatgacagg agggcgctcc
      961 cgaccgcctg agggggttcc agctcctcgg tgaagaggaa gttgccgagt gaataggcga
     1021 tgagtgagga gcccctggct tcgagaccct gaaggacatg ggggtggctg ccgatcacga
     1081 gcctggcgcc cgcgtcggca aacccgcggt agagctcgcg ggtctcggcc gtggggctct
     1141 ccacgtattc ccttccccca tgggccaaga cgatcacggt gtcccctgcg gccgcggcta
     1201 tcctgatcgc gtcgagggtg gagcggggat cccccgcgat gccgggtttg gcggctccag
     1261 ccagagcctc ggcggtcgta aagcccagac gctcggtcgg atagctggcg aagcccacga
     1321 agctgagcct ctgcccccca ggcaaggcgg ccagcacgcg gggctgaccg gcctcctcct
     1381 cgttccgccc cgcgcccacg aagggcatgg ccgcgccctc aaggtcctca agggtatcga
     1441 ggaagccctc ctcgccgaaa tcgaagccgt ggttgttggc gaaaaggagg agggagaagc
     1501 cggcatcgcc gagggccttg gcgcttcccg gtgggaaccg gaaatggaag cgcttgcgcg
     1561 ggttggggct gccccgggcg gtgatcgggc cctcgaggtt ggccacgagg agatccctcg
     1621 acctcatgag gggaaggagg cccccggcga agacccgctc caggccagcc tcgccgccga
     1681 tgagggaaag cccggagcgc ggaccgacct gaagatcgcc ggcggcgccg agtacgatgg
     1741 gggccggctc ggccttcgcg gccgcggcgg ccccggccag ccaggaggcg aggacagggg
     1801 gaatccgcct ccggctcgag agccgaagtt cgagccgctc tacgaagggg tagccgggct
     1861 ggcccggcca tctgccgtcg acggaggcgg cccgccgcgg gggcaggatc tcgtcgaggg
     1921 cctcgagtcc ggcggcgcgg gcctgggccg aatcgaggcg gtagaggggg ctcgagatgg
     1981 gcagggccgg ggcgagccag aggaggccgg catccgcgct gagggggccg cccgtttcgt
     2041 gcgcccctgg ctgttgggca gggtcagcgg gcgtggccac aaggctcagg gtcgcctcgc
     2101 cgtccgcctc cgtcgggacc caggccttgg gaagggggct ggcttcgagg atctcggcga
     2161 gccgggccct gagccctggg cccgccgaaa gctcgatcct gccccccgtg gccgcgcagc
     2221 cggcgttgag gcagagggcg aggaggccga ggacgggggg caaaggccgg cgccgaggat
     2281 ggcgggggcc gggcttcggg ctgggcgggg tagggaaccc caccctcagc cccgccttgt
     2341 gcggaccata cccagctgga gggcgaggcc ggcggccatg aggagggcga ggaagatgaa
     2401 gacccatgac cacgagacaa gctgatagac gacgcctccg gctatcgagc cgaggatctg
     2461 gccggcggtg aggagggcct cgtggatggt gacactcctg tcgcggtcgg gggccccgct
     2521 tgcgccgtag aacagggac
//
LOCUS       DASSQV010000331         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2069288, whole genome shotgun sequence.
ACCESSION   DASSQV010000331 DASSQV010000000
VERSION     DASSQV010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069288"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFL04_05770"
     CDS             complement(<1..469)
                     /locus_tag="VFL04_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839246.1"
                     /translation="MPTLKKYKKMENAFYAKASARLTASGGRFRAFLARVGVAGRQKL
                     TIMLIPHTEKKIINLQLSFFAMAGLIAAAAIVLFAFVFSAARFSDTARRLQNRSDDLK
                     STQADLDSIRDETSRLVTAAKRFETSLSGTLARIGAQSSAPDSQVQQGDLASFF"
     gene            638..1504
                     /locus_tag="VFL04_05775"
     CDS             638..1504
                     /locus_tag="VFL04_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7839247.1"
                     /translation="MSVAQAETGTKGALSLSNEGELALTFIGAGSAFSRKFYQNNILL
                     VKGDSHLLVDCGTRTPEALSMLGLSVGKIHNYLVTHSHADHIGGLEEVMLVNRYGLRQ
                     KTTMVVTEKLRKILWTMSLRGGASWNEVREGRPLEFEDFWNVISPAKVPKAGRELCAA
                     EVGSLSLKLFRTKHIPDSAAGWSDSFPSYGLIIDDRILYTADTRYDPEMVIEMDREYR
                     FETIFHDCQFFTGGVHASLEELAGLPADIRERTLLMHYGDNAEAFRSRITELGFRGLV
                     EQWKTYAFPTRA"
     gene            1597..>2059
                     /locus_tag="VFL04_05780"
     CDS             1597..>2059
                     /locus_tag="VFL04_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7839248.1"
                     /translation="MAQHHFKTEVSRLLDLIIHSLYSHKEIFIRELISNSSDALDKLR
                     YLTISDEAFRGFAFEPAIRVVLDEGAKTLVVEDNGLGMDEKELEDNLGTIARSGTKRF
                     MEKLPEDAKKDSNLIGQFGVGFYSAFMVAKKVDVLTRRAGSAEAWLWSSTGE"
BASE COUNT          406 a          670 c          647 g          336 t
ORIGIN      
        1 cgaagaacga ggcgaggtcg ccctgctgca cctgggagtc cggcgccgag ctctgggccc
       61 cgatccgggc cagggtgccc gagagcgacg tctcgaaacg cttggccgcg gtgaccagcc
      121 tcgaggtctc gtcgcggatc gagtcgaggt ctgcctgggt cgacttgagg tcgtcgctgc
      181 ggttctggag gcggcgcgcc gtgtccgaga acctcgccgc cgagaacacg aaggcgaaaa
      241 ggactatcgc ggcggcggcg atcaggcccg ccatcgcgaa gaagctgagc tggaggttga
      301 ttatcttttt ttcagtatgg gggatgagca tgatggtcag cttctggcgg cccgcgacgc
      361 ccacgcgcgc gaggaaggcc ctgaagcgac cgccggaggc ggtgaggcgg gcggaggctt
      421 tcgcgtagaa agcgttctcc atctttttgt acttcttcag agtgggcacc accatctcct
      481 cgccctagat ataaagaaaa gactccgcct tcgatgatac tctagcattc caagtatcgg
      541 actgtcaaag ggcaaaagcc agcggacggg ggccgaaagg gcattcagga gcggcgcgga
      601 atttgattcg ccgccgcgga acgctataat ggtgagaatg agcgtcgcgc aggccgaaac
      661 cgggacgaag ggggcattgt cgctctcgaa cgagggggag ctcgccctga ccttcatcgg
      721 ggccgggagc gctttctcca ggaaattcta ccagaacaac atcctcctcg tgaagggcga
      781 ctcccatctc ctcgtcgact gcgggacgcg gaccccagag gccctctcca tgctcgggct
      841 ctccgtcggc aagatccaca actacctggt cacccacagc cacgccgacc acatcggcgg
      901 cctcgaggag gtcatgctcg tcaaccgcta tggcctccgc cagaagacca ccatggtcgt
      961 caccgagaag ctgcggaaga tcctgtggac catgagcctc aggggcgggg cctcctggaa
     1021 cgaggtgcgc gagggcaggc ccctggagtt cgaggacttc tggaacgtca taagcccggc
     1081 caaggtgccc aaggcgggaa gggagctctg cgcggccgaa gtcggctcgc tctcgctgaa
     1141 gctcttccgg accaagcaca tccccgactc ggcggcgggc tggagcgact ccttcccgag
     1201 ctatggcctc atcatagacg acaggatcct ctacacggcc gacacgaggt acgacccgga
     1261 gatggtcatc gagatggaca gggagtaccg cttcgagacc atattccacg actgccagtt
     1321 cttcaccgga ggggtccacg cctcgctcga ggagctggcc gggctcccgg ccgacatacg
     1381 ggagcgcacc ctcctcatgc actacggcga caacgccgag gccttccgct cgcggatcac
     1441 cgagctcgga ttccgaggcc tcgtcgagca gtggaagacc tacgccttcc cgaccagggc
     1501 ctgaaaccgg ccggggcgaa gccttgcccg ccccgcggct aatcggtatc ttaagcagcg
     1561 cacccacacc accatacact tcacgggaga gcccgcatgg cacagcacca tttcaagacc
     1621 gaggtctcgc gcctcctcga ccttatcatc cactccctgt actcgcacaa ggagatcttc
     1681 atcagggagc tcatctcgaa ctcctccgac gccctggaca agctccgcta tctcacgatc
     1741 tcggacgagg ccttccgggg cttcgccttc gagccggcga tccgcgtcgt cctggacgag
     1801 ggcgccaaga ccctggtcgt cgaggacaac gggctcggca tggacgagaa ggagctcgag
     1861 gacaacctcg gcaccatcgc ccgctcgggc accaagcgct tcatggagaa gctgcccgag
     1921 gacgcgaaga aggactcgaa cctgatcggc cagttcggcg tcggcttcta ctcggccttc
     1981 atggtggcaa aaaaagtgga cgtgctgacc cgccgcgccg gctccgccga agcctggctg
     2041 tggtcgtcca ccggcgagg
//
LOCUS       DASSQV010000332         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_701334, whole genome shotgun sequence.
ACCESSION   DASSQV010000332 DASSQV010000000
VERSION     DASSQV010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701334"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1846)
                     /locus_tag="VFL04_05785"
     CDS             complement(<1..1846)
                     /locus_tag="VFL04_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET7839249.1"
                     /translation="MYFEELVAAYDKARAERNVFHDLMRYRVREVLLVASLYDSFIVE
                     SDGVLTEQIYGEYYKLNLSAVPRITCAYSPEDAIAYFATGRFDLVILMAGLDFDRPLA
                     LARAMKERQAEVTVLLMVTNNSALSALDMGRPELGSVDRVFVWNGYSKLFIGMIRHVE
                     DLRNVDADTRTGMAKVILLIEDSVRYYSRYLPLLYQVVLRQTQSLIEEERGGETWKLL
                     RSRARPKVLLASSYEEAARLFELYEPYILTVITDIRFPRGGVLDPEAGFDFIRMAKSR
                     IVDLPVMIQSSEAEVRERAWALDASFADKSSESLEHQLAAYLQTSLGFGSFKFRSPTG
                     ELIGEAQDLEEFLARLHDVPPESVVFHASRNHFSAWLMARGEIQFARLLRPYQVEDFG
                     SAFHLKDFILRFIDRAVRERSRGAVPYFDGGSCRDENCAVRLGDGSVGGKGRGITFLR
                     SLLDNFDFARHIEGLELKVPRSAFIGIDEFERFLEQNGLWAYAFYQASSEEVRSRFLE
                     APLSATLVDRLRRFAEYSAKPLAVRSSGLFEDMLMVPFSGIYATYILPNSDPDPERRL
                     AQLCDAVRLIYASLFSERARAYFDTAGYKLEEERMAVVVQELAGSRHGR"
     gene            2505..>2818
                     /locus_tag="VFL04_05790"
     CDS             2505..>2818
                     /locus_tag="VFL04_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839250.1"
                     /translation="MSREVLELDVGHVAFPYQIQALAEICLEADFEVFRFRRVFGNSA
                     HIVYLRAQYSSTRHRAQQKMTIILDVWRNVTYCDFMYSTLVSRETVTDIRYGDKEAGF
                     GL"
BASE COUNT          509 a          842 c          970 g          497 t
ORIGIN      
        1 atcggccgtg ccgggagccg gcgagctcct ggacgacgac ggccatcctc tcctcctcga
       61 gcttgtagcc tgccgtgtcg aaataggccc tggcccgttc ggagaagagg ctcgcgtaga
      121 tcagacgtac ggcgtcgcag agctgggcga ggcggcgttc ggggtcgggg tcggagttgg
      181 gcagaatgta ggtcgcatag atgccggaga agggcaccat cagcatgtcc tcgaagaggc
      241 ccgaggagcg gaccgcgagg ggcttggcgc tgtattcggc gaaacgccgg agccggtcca
      301 cgagggtcgc ggagaggggg gcctcgagga aacgggagcg cacctcctcg ctcgaggcct
      361 ggtagaaggc gtaggcccag agcccgttct gctcgaggaa gcgctcaaac tcgtcgatcc
      421 cgatgaaggc cgaccgcggg accttgagct cgagcccctc gatgtggcgg gcgaaatcga
      481 agttgtcgag gaggctgcgg aggaaggtga tgcccctccc cttccccccg accgagccgt
      541 cgccaaggcg gacggcgcag ttctcgtccc tgcagctgcc cccgtcgaaa tagggcacgg
      601 ccccgcggga gcgctcccgg acggcgcggt cgatgaagcg gaggatgaag tccttgaggt
      661 ggaaggccga gccgaagtcc tcgacctggt agggccgcag gaggcgggcg aactggatct
      721 cgccgcgggc catgagccag gccgagaagt ggttgcggct cgcgtggaag acgacgctct
      781 cgggcgggac gtcgtggagc cgggcgagga actcctcgag gtcctgggcc tcgccgatga
      841 gctcccccgt gggcgagcgg aacttgaagg agccgaagcc caggctcgtc tggaggtagg
      901 ccgcgagctg gtgctcgagg gactccgagc tcttgtcggc gaaggaggcg tcgagggccc
      961 aggccctctc cctcacctcg gcctcgctcg actggatcat cacgggcagg tcgacgatgc
     1021 gggacttggc catgcggatg aagtcgaagc cagcctcggg gtcgaggacc ccgccgcggg
     1081 ggaagcggat gtcggtgatg acggtgagga tgtagggctc gtagagctcg aagagcctgg
     1141 cggcctcctc gtagctcgag gcgaggagga ccttgggcct ggcccggctg cgcaggagct
     1201 tccaggtctc ccctcccctc tcctcctcga tgaggctctg ggtctggcgc aggaccacct
     1261 ggtagagcag gggcaggtag cgcgagtagt agcgcaccga gtcctcgatg aggaggatga
     1321 ccttggccat gccggtgcgg gtgtcggcgt cgacgttgcg gaggtcctcg acgtgcctga
     1381 tcatcccgat gaagagcttc gagtagccgt tccacacgaa gaccctgtcc acggagccga
     1441 gctcggggcg gcccatgtcg agggccgaga gggcgctgtt gttggtgacc atgaggagga
     1501 cggtcacctc ggcctggcgc tccttcatgg ccctggcgag ggccaggggc ctgtcgaagt
     1561 cgaggccggc catgaggatg acgaggtcga agcggccggt cgcgaaatag gcgatggcgt
     1621 cctcgggcga gtaggcgcag gtgatccggg gcacggcgct caggttgagc ttgtagtact
     1681 cgccgtagat ctgctcggtg aggaccccgt cggactccac gatgaaggag tcatagaggc
     1741 tcgcgaccag gaggacctcg cgcaccctat agcgcatgag gtcgtggaag acgttccgtt
     1801 cggccctcgc cttgtcgtag gcggcgacca gttcctcgaa gtacatgcca agggattatc
     1861 cgcgaagcgc ccggccttat caatgcggcg gaaacagagg ggccgccccc tccaccggga
     1921 gtccgggcgg ccccatcccc agctcggcgg cgccgcggcg cctcgcacgg ggattctcgt
     1981 tctcaaacgc gaatatgtcg aagaatgaca agctcatcct gtgatcgact aagctaacga
     2041 ttcgaagcgt cgaaaggttt tacctggttt tgggcgctcg gttcgcgcca cgagactcgc
     2101 cctagggcga ccgaatgggg gagacagaag ccttctctct cgcgctgccg cagttccaat
     2161 caagtccgat cgagttttcc gaaaccctca aagccgcgca cactacccgc gtcctgacgc
     2221 gcacaagcaa cctccattcg aagttcattc tcccacctcg ctccacggat cgtcgttgtc
     2281 cgttccccaa acgcgcggag gcggcttcct gaattgcatg attcttcctt cgtcgcccgg
     2341 gaaggtctca cgagccagag acctcatttc ccgcagtggt atggggatca ctctcccacg
     2401 gccgggcgtt gtggttccaa tagctgcctc tgggcgatgt cgcgcatcgt cttctcgagg
     2461 tcggccgagg accggatcag ggcctcaagt cccagcctca gcagatgagc cgcgaagtcc
     2521 tggagctgga tgtcgggcat gtggctttcc cgtaccagat ccaggcgctc gcggagatct
     2581 gccttgaggc ggattttgag gtattccgat ttaggcgcgt ctttggaaat tctgcccata
     2641 tcgtgtacct ccgggcacag tatagcagca ctaggcacag agcgcaacaa aaaatgacga
     2701 ttatccttga cgtgtggcgt aatgtgactt attgtgactt tatgtactca acactcgtat
     2761 cacgggagac cgtcacggat atcaggtatg gcgacaagga ggccggcttc gggctctg
//
LOCUS       DASSQV010000333         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2069480, whole genome shotgun sequence.
ACCESSION   DASSQV010000333 DASSQV010000000
VERSION     DASSQV010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069480"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            468..1493
                     /locus_tag="VFL04_05795"
     CDS             468..1493
                     /locus_tag="VFL04_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7839251.1"
                     /translation="MAIRIKDIAEMAGVSPATVSLVLNSKPGAGEGVRERVLRIAKEQ
                     GYVPLRSSSSAPAGETACFLHIARHGHTINRDHDVFIADYIEGLGQGAKQHGLGLEVL
                     TYKSTPVELIVEAAQASQAEGIVILGTELSASDVEAFSAVRKPLVFLDTYHDFLPFDF
                     VDMNNGDAAFTVVSYLKELGHRSIGLVKGEIETRNFRLREEGFVASLDRLGLDFDPGL
                     VFSVDQTFHGAYEGMLALLKGGTSLPSALFCENDIIACGVLRALKEKGVRVPEDISLV
                     GFDDLPLSSVVDPPLTTIQVSKAQIGRTAIQLLQTRIRSEADEPPSKVLIGGRLVVRQ
                     STRAVPA"
     gene            1517..2491
                     /locus_tag="VFL04_05800"
     CDS             1517..2491
                     /locus_tag="VFL04_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET7839252.1"
                     /translation="MVKYSVILGNLGNTCDRFLSTGYKDQLDKAEMVRQAAAIPGVKG
                     IELVGTWDVTPQNVEEVGGLLAKHGLACVSIIPDHFSQKRWGRGAFTAKDPAIRAQAL
                     EETMAAAEMARALKCPLINLWPGQDGYDYVLQGDYRQERRWIIEAVAKAAKAYPDIGF
                     SLEYKPKEPRTHSYMARAADTLLVAKETGLPNVGVTIDTGHSFVAGENVAEAAVLLSD
                     YGKKLFHMHFNDNYRGWDDDMIVGSVHCAEFVELLFWLKETGYSGWYSMDQYPYREDG
                     QGALRGSVEFLMRIDEVLDAKAMAELRALIAKGDAVESVAWLRSKLFR"
     gene            complement(2504..2911)
                     /locus_tag="VFL04_05805"
     CDS             complement(2504..2911)
                     /locus_tag="VFL04_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HET7839253.1"
                     /translation="MSPELLKILHEMGHGDELVIGDSNYPAASTAQRLVRADGHGLPE
                     LLDAILRLFPLDSYVEKPVLLMQVVPGDPTVPTVHEAIKEVVRRHEPRGDRAVGGIER
                     FEFYERAKASYAVLATTEKGAYGCVIIKKGVVR"
BASE COUNT          510 a         1001 c          922 g          480 t
ORIGIN      
        1 ctgtccttgg gcacgagggc gattttccac tggctttggg gcttggacga cttgcccacc
       61 tgggcgaaga gcggcatggc gatgctcatg acgaacacga gaccgagaat cgtgacgagc
      121 gatttcttca tgaggtccct ccttgggaag atggcgatgg gccaattgta gcatcgcccc
      181 ggcccctgtc aagttttagt gatgattgac agggggtttt agtcggaaat gctgaggctc
      241 cgccgcgggc cggctcggcc cgggacatgg ctcgaccgcc cttccagcgt aattgcttac
      301 ctgttatgat gcaaaaggat atgactgtct cggccttgag cctcggcccg aatgagggcc
      361 actaaactcg ccactgtgct taactaaatc cgccaagtct tccttgacaa gacgccgggc
      421 cgggtctact ttggggcgaa gatctgcggc ctaaggaggg cgggctcgtg gccattcgaa
      481 tcaaggacat cgccgagatg gccggagtct cgcccgccac cgtctccctc gtcctcaaca
      541 gcaagccggg ggccggcgag ggcgtgcgcg agcgcgtgct ccgcatcgcc aaggagcagg
      601 gctacgtccc cctccgcagc agctcctcgg cccccgccgg cgagaccgcc tgcttcctcc
      661 acatagcccg ccacggccac acgatcaacc gcgaccacga cgtcttcatc gccgactaca
      721 tcgagggcct cggccagggc gccaagcagc acgggctcgg gctcgaggtc ctcacctaca
      781 agtcgacccc cgtcgagctg atcgtcgagg ccgcccaggc ctcgcaggcc gagggcatcg
      841 tcatcctcgg caccgagctc tcagcctccg acgtcgaggc cttcagcgcc gtgcgcaagc
      901 ccctcgtctt cctcgacacc taccacgact tcctgccctt cgatttcgtc gacatgaaca
      961 acggggacgc ggccttcacc gtggtctcct acctcaagga gctcgggcac cggagcatcg
     1021 gcctcgtgaa gggcgagatc gagacgcgca acttccggct cagggaggag ggcttcgtcg
     1081 cctcgctgga ccggctcggc ctcgacttcg acccgggcct cgtcttctcg gtggaccaga
     1141 ccttccacgg ggcctacgag ggcatgctcg ccctcctcaa ggggggcacg agcctgccca
     1201 gcgcgctctt ctgcgagaac gacatcatag cctgcggggt cctcagggcc ctcaaggaga
     1261 agggcgtcag ggtccccgag gacatctccc tcgtcggctt cgacgacctg cccctgtcct
     1321 cggtcgtcga cccgcccctc acgaccatcc aggtctcgaa ggcccagatc ggccggacgg
     1381 ccatccagct gctccagacg agaatacgct cggaggcgga cgagccgccg agcaaggtac
     1441 tcataggggg gcgcctcgtc gtgcggcaga gcacccgcgc cgtcccagcc tgaacgggat
     1501 cctaaggagg gaatcgatgg tcaagtattc tgtgatcctc ggcaatctcg gcaacacctg
     1561 cgaccgcttc ctgtccacgg gctacaagga ccagctcgac aaggccgaga tggtgcgcca
     1621 ggccgcggcc atccccgggg tcaagggcat cgagctcgtc ggcacctggg acgtcacgcc
     1681 ccagaacgtc gaggaggtgg gcggcctcct cgcgaagcac ggcctcgcct gcgtctccat
     1741 catccccgac cacttctcgc agaagcgctg gggccgcggg gccttcaccg cgaaggaccc
     1801 cgccatcagg gcccaggccc tcgaggagac catggcggcc gccgagatgg cgagggccct
     1861 caagtgcccc ctcatcaacc tctggcccgg ccaggacggc tacgactacg tcctccaggg
     1921 cgactaccgc caggagaggc gctggatcat cgaggccgtg gccaaggccg ccaaggccta
     1981 ccccgacatc ggcttctcgc tcgagtacaa gcccaaggag ccccggaccc actcctacat
     2041 ggccagggcc gccgacaccc tcctcgtcgc gaaggagacc ggactgccga atgtcggcgt
     2101 caccatcgac acggggcatt ccttcgtcgc cggggagaac gtggccgagg ccgccgtcct
     2161 cctctcggac tacggcaaga agctcttcca catgcacttc aacgacaact accgcggctg
     2221 ggacgacgac atgatcgtgg gctcggtcca ctgcgccgag ttcgtcgagc tcctgttctg
     2281 gctcaaggag accggctatt cgggctggta ctcgatggac cagtacccct accgcgagga
     2341 cggccagggc gccctccgcg gctcggtcga gttcctcatg aggatcgacg aggtcctgga
     2401 cgcgaaggcg atggccgagc tcagggccct catcgccaag ggcgatgccg tcgagtcggt
     2461 ggcctggctg aggtccaagc tgttcaggta gtcagaagac gcctcagcgc acgacgccct
     2521 tcttgatgat gacgcagccg taggctccct tctcggtggt ggccaggacg gcgtaggagg
     2581 ccttggcgcg ctcgtagaac tcgaagcgct cgatgccgcc caccgccctg tccccgcgcg
     2641 gctcatggcg gcggacgact tccttgatcg cctcgtgcac ggtcggcacc gtgggatcgc
     2701 cgggcacaac ctgcatcagg aggacgggct tctcaacgta gctgtccagg gggaagagcc
     2761 tgaggatggc gtcgaggagc tcgggcagac cgtggccgtc ggccctcaca agacgctggg
     2821 ctgtgctcgc cgcggggtag ttcgagtcgc cgatgacgag ctcgtcgccg tggcccatct
     2881 cgtggaggat cttgaggagc tcgggagata tga
//
LOCUS       DASSQV010000334         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1446018, whole genome shotgun sequence.
ACCESSION   DASSQV010000334 DASSQV010000000
VERSION     DASSQV010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446018"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFL04_05810"
     CDS             <1..414
                     /locus_tag="VFL04_05810"
                     /inference="COORDINATES: protein motif:HMM:NF019031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HET7839254.1"
                     /translation="EKVMVVEEGQETPMHFHWSKMEDIIVRGGGSLAVELYRSTPEGG
                     LSDEPVLLRVDGVLRELPPGGRVVLGPGESVCLESGVYHRFWGEGARVLVGEVSMVND
                     DLSDNRFLEDCGRFPEIEEDEEALHLLVCDYPRKA"
     gene            complement(449..1387)
                     /locus_tag="VFL04_05815"
     CDS             complement(449..1387)
                     /locus_tag="VFL04_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7839255.1"
                     /translation="MNAPEPTRGGYLAVCLNPVIQKTLVYPRLVPGEVNRTAEHRVDV
                     AGKGICVTRVLGQLGRRVVHLTQLGGPTRDWFLDMCEADGLELSWVESGSEIRFCTTL
                     IDQGSGEATELVEEARPVQPGTAERVLAQFSALLPACATVMLSGTVAAGFPADIMPRM
                     AALAAAAGKRLFLDLKGRALLDSLSARPTLVKPNLEELYQTYEPGRSRPGVDADEASI
                     RELVSRAGREYRERYGSWLVVTRGTEPTLFWDGQGLRSAPVQAAKALNPIGSGDSFNV
                     GVATALEDGATIAEAVAEGNRLGALNAERLKPGSIA"
     gene            1509..2150
                     /locus_tag="VFL04_05820"
     CDS             1509..2150
                     /locus_tag="VFL04_05820"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET7839256.1"
                     /translation="MQEIFAALRARGIVPVIKLEKAETALPLAAALIEGGLPVAEITF
                     RTAAAAEAISLIRRNHPSILLGAGTLTTPEQVEAAIEAGASYAVSPGFNPRIVELCQK
                     RGLPIIPGVNNPSQVEQGLERGLDLLKFFPAEASGGVKMLKALHGPYAEVSFVPTGGV
                     ELSNLESYLGLPYVAAIGGTWMVKEDLIAGGRFKEIAELCRAAVDLAAAIRKN"
     gene            2178..>2852
                     /locus_tag="VFL04_05825"
     CDS             2178..>2852
                     /locus_tag="VFL04_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET7839257.1"
                     /translation="MKRYILAHDLGTSGDKATIFDLEGGLVASFTSAYPTRFFNGTWA
                     EQEPEEWWRAVVVSTRRVAAAVDPAEIGAIGFSGQMMGCVCVDEAGRPLRPSLIYCDQ
                     RATAQEAELLGAVDAHEFYRITGHRASASYPAAKLMWLRDHEAGTYAATAAMLQAKDW
                     LNFRLTGVMRSEYTDASGTGLLDLGALAWSERLVAAAGLDGEKLPALVGSTEVVGELT
                     GAAADEL"
BASE COUNT          416 a          991 c         1039 g          406 t
ORIGIN      
        1 gagaaggtca tggtggtcga ggagggccag gagaccccga tgcacttcca ctggtccaag
       61 atggaggaca tcatcgtgcg cggggggggc tccctcgcgg tcgagctcta ccgctcgacg
      121 cccgagggcg ggctttcgga cgagccggtg cttcttcggg tggacggggt cctccgcgag
      181 ctgcccccgg ggggtcgggt ggtcctgggg cccggcgaga gtgtctgtct cgagagcggg
      241 gtctaccacc gcttctgggg cgagggggcc agggtcctcg tgggcgaggt gagcatggtc
      301 aacgacgacc tcagcgacaa ccgcttcctc gaggactgcg gccgcttccc cgagatcgag
      361 gaggacgagg aggccctcca cctcctcgtc tgcgactacc cgcggaaggc ctgagccccg
      421 gcccggcctg agccccggcc ccggcgcctt aggcgatcga gccgggcttg agccgctcgg
      481 cgttcagggc gccgagcctg ttgccctcgg cgacggcctc ggcgatcgtg gccccgtcct
      541 cgagtgcggt ggcgacccca acgttgaagg agtcgcccga gcctatgggg ttgagggcct
      601 tggccgcctg gaccggggcc gagcggaggc cctggccgtc ccagaagagg gtcggctcgg
      661 tgccccgggt gacgacgagc caggagccgt agcgctcgcg gtactccctg cccgcccggc
      721 tcacgagctc gcggatcgag gcctcgtcgg cgtcgacccc gggtctgctg cggccgggct
      781 cgtaggtctg gtagagctcc tcgaggttgg gcttcacgag ggtcggcctc gcgctgaggc
      841 tgtcgaggag ggccctgccc ttcaggtcga ggaagaggcg tttgccggcg gccgcggcga
      901 gggcagccat ccggggcatg atgtcggccg ggaagccggc cgcgacggtg cccgagagca
      961 tcaccgtggc gcaggcgggg aggagggcag agaactgggc gagcacccgc tcggccgtgc
     1021 cgggctggac cggcctggcc tcctcgacga gctcggtggc ctcgcccgag ccctggtcta
     1081 tcagggtggt gcagaagcgg atctccgagc ccgactcgac ccaggagagc tcgagcccgt
     1141 cggcctcgca catgtcgagg aaccagtccc tcgtggggcc gccgagctgg gtgaggtgga
     1201 ctaccctgcg gccgagctgg ccgaggaccc tggtcacgca gatgcccttg cctgccacgt
     1261 cgacccggtg ctctgccgtc ctgttgacct cgcccggcac gaggcgggga tagacaaggg
     1321 tcttctggat cacggggttg aggcagacgg cgaggtagcc gccgcgtgtc ggctcggggg
     1381 cgttcatggc gcgactatag cccctttcaa gctgggacga aaagcggcta ggatgggcgg
     1441 cggaaaaggg cgcagccatc gccgcgcgat ggccgggagc gcccccgcaa ggaaaaggag
     1501 cacaaagcat gcaggaaatc ttcgcggccc tccgcgcacg ggggatcgtc ccggtgatca
     1561 agctcgagaa ggccgagacc gccctgcccc tggccgcggc cctcatcgag ggcgggctgc
     1621 cggtggccga gatcaccttc cgcaccgccg ccgccgccga ggccatcagc ctcatccgca
     1681 ggaaccaccc ctcgatcctc ctcggcgccg gcaccctgac gacgcccgag caggtcgagg
     1741 ccgcgatcga ggccggggcc tcctatgccg tgagccccgg cttcaatccg cgcatcgtcg
     1801 agctctgcca gaagcgcggc ctgcccatca tccccggggt gaacaacccc agccaggtcg
     1861 agcagggcct cgagcgcggc ctcgacctcc tgaagttctt ccccgccgag gcctcgggcg
     1921 gggtcaagat gctcaaggcc ctccacggcc cctacgccga ggtctccttt gtcccgacgg
     1981 gcggggtcga gctctcgaac ctcgagtcct acctcggcct cccctacgtg gccgcgatcg
     2041 gcggcacctg gatggtcaag gaggacctca tcgccggcgg gaggttcaag gagatagccg
     2101 agctctgcag ggccgccgtc gatctggcgg cggcgatcag gaagaactag gcccgggcgg
     2161 ggctacgggg aggctccatg aagcgctaca tcctggccca cgacctcggc acctcgggcg
     2221 acaaggccac catcttcgac ctcgagggcg gcctcgtcgc gagcttcacc tcggcctatc
     2281 ccacccgctt tttcaacggc acctgggccg agcaggagcc cgaggaatgg tggagggccg
     2341 tcgtcgtatc cacccgccgg gtggccgcgg cggtcgaccc cgccgagatc ggggccatcg
     2401 gcttctcggg gcagatgatg ggctgcgtct gcgtggacga ggcggggagg ccgctccggc
     2461 cctccctcat ctactgcgac cagagggcga cggcccagga ggccgagctc ctcggggcgg
     2521 tggacgcgca cgagttctac cgcataacgg gccaccgagc ctcggcctcc taccccgcgg
     2581 ccaagctcat gtggctcagg gaccacgagg ccgggaccta cgcggcgacg gccgccatgc
     2641 tccaggccaa ggactggctc aacttccggc tcacgggagt catgcgttcg gagtacacgg
     2701 acgcctcggg cacgggcctc ctcgacctcg gcgccctcgc ctggtcggag cggctcgtgg
     2761 ccgccgcggg cctcgacggg gagaagctgc ccgccctcgt gggctcgacc gaggtcgtcg
     2821 gcgagctcac gggcgcggcc gccgacgagc tt
//
LOCUS       DASSQV010000335         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1323199, whole genome shotgun sequence.
ACCESSION   DASSQV010000335 DASSQV010000000
VERSION     DASSQV010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323199"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VFL04_05830"
     CDS             complement(<1..1112)
                     /locus_tag="VFL04_05830"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HET7839258.1"
                     /translation="MAVAYFPARLPARAGPGPCRPSHLRIQAYAAKEFLFGFSVCFLF
                     FFAVFFVNQILLMAEDILSKKAPLRDVILLLVYAMPSVIAMSFPFASLVGALMAAGRL
                     SSDNEFLALMASGVPARETFLPFVILGLAFSIASFTMNDYFLPRGAIEFGKLYRKLIS
                     STPALELKPWSVKRYADIAVVTGEQVGNDLHNLLVFDHGAEGKARVISASVARLVSAG
                     ATGEVLLQLEGVWFQTVKHDETDRFEWAQAKSMEYRFSTKDSGEDAISVGPREMASVD
                     LARVIAEKRSLLEEKRLRREYDLASARAGLEDSYEADLVAGLAWANAAERAGPRLSAI
                     RALGPAPPEDRSLQVYTLELYKKFSIPFGALCFVVLA"
     gene            complement(1117..1542)
                     /gene="dut"
                     /locus_tag="VFL04_05835"
     CDS             complement(1117..1542)
                     /gene="dut"
                     /locus_tag="VFL04_05835"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET7839259.1"
                     /translation="MRLHVEEGASVPSYQSPGSAGADLCAFLPSPVALEPGARMAVPT
                     GLRMELPEGYEAQVRPRSGLALKHGISCLNSPGTIDADYRGELKVILANLGSEPFIIS
                     PGDRIAQLIVAPVSRASFVVTDRLGSTSRGEGGFGSTGR"
     gene            complement(1550..>2353)
                     /locus_tag="VFL04_05840"
     CDS             complement(1550..>2353)
                     /locus_tag="VFL04_05840"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET7839260.1"
                     /translation="PEDIRRITRDALYEFYRRSLRPESLLATTAGPVPLDEVERELGA
                     LLGGVLISAEAAATRAEMTPAFRSVSSYLAADMEQVHYYEALQLEPPFAMKDYLALAA
                     LNGALGEASSSRLFQSLRERLGLCYSVYSAFSLDASECLWMASANVSTKQLPRLAAEL
                     ARELDAVASNGLEPQECRDSVSRLAGSFEISLDDPDFRMRRIARQILFAGRALDIEET
                     RARIQAVSLDEVNAMCARLLSGRDRARFAYGRRSGPAARALGLEEVGGE"
BASE COUNT          391 a          774 c          844 g          344 t
ORIGIN      
        1 gcgagcacca cgaaacagag ggcgccgaag ggtatggaga atttcttgta aagctcgagg
       61 gtatagacct ggaggctgcg gtcctcgggc ggggctggcc cgagggctcg gatggccgag
      121 aggcgggggc cggcccgctc ggccgcgttg gcccaggcga ggcctgcgac gaggtcggcc
      181 tcgtaggagt cctcgagccc ggcccgggcc gaggcgaggt cgtactcgcg ccggagccgc
      241 ttctcctcga gaagggagcg cttctcggct atcaccctgg ccagatcgac cgaggccatc
      301 tccctcggcc ccaccgagat cgcgtcctcg cccgagtcct tcgtggagaa cctgtactcc
      361 atcgatttcg cctgggccca ctcgaagcgg tcggtctcgt catgcttcac ggtctggaac
      421 cagaccccct cgagctggag cagcacctct cccgtggcgc ccgccgacac gaggcgggcg
      481 accgaggccg agatgacacg ggccttgccc tcggcgccgt ggtcgaagac gaggaggttg
      541 tgcaggtcat tgcccacctg ctcgcccgtg acgacggcga tgtcggcgta gcgcttgacc
      601 gaccagggct tgagctcgag ggccggggtc gagctgatga gcttgcggta gagcttgccg
      661 aactcgatgg cgcccctggg caggaaatag tcgttcatcg tgaaggaggc gatcgagaag
      721 gccaggccga ggatcacgaa ggggaggaag gtctcgcgcg ccggcacgcc cgaggccatg
      781 agggcgagga actcgttgtc ggaggagagg cggccggccg ccatgagggc tcccacgagg
      841 gaggcgaagg gaaaggacat ggcgatcacc gatggcatcg cgtacacgag gaggaggatg
      901 acgtcccgga gcggggcctt cttcgagagg atgtcctcgg ccatgaggag gatctggttc
      961 acgaagaaga cggcgaagaa gaacaggaag cagaccgaga agccgaagag gaattccttc
     1021 gcggcgtagg cctgtatgcg gaggtggctc gggcggcacg gccccggccc cgccctggct
     1081 ggaagcctcg ccggaaaata ggccaccgcc atgcagctag cgccccgtcg atccgaatcc
     1141 gccctcgccg cgcgaggtcg atccgagcct gtccgtcacg acgaagctcg ccctcgagac
     1201 cggggcgacg atgagctggg cgatcctgtc gcccggggag atgatgaagg gctcagagcc
     1261 aagattggcg aggatcacct tgagctcgcc gcggtagtcg gcgtcgatgg tgccgggaga
     1321 gttgaggcag ctgatcccgt gcttgagggc caggcccgac cggggcctca cctgggcctc
     1381 gtatccctcg ggcagctcca tcctgagccc cgtgggcacc gccatcctcg ccccgggctc
     1441 gagggcgacc ggggacggga ggaaggcgca gaggtcggcc cccgcgctcc ccgggctctg
     1501 gtagctgggg accgaggccc cctcctcgac atgcagcctc accacgggct cattcaccgc
     1561 cgacctcctc caggccgagg gcccgcgcgg ccggccccga ccttcgcccg taggcgaacc
     1621 tggcccgatc cctccccgac aggagccttg cgcacatcgc gttcacctcg tccaggctca
     1681 cggcctggat gcgggccctc gtctcctcga tgtccagggc cctgcccgcg aagaggatct
     1741 gcctggctat cctgcgcatc cggaagtccg ggtcgtcgag gctgatctcg aagctgcccg
     1801 cgagcctgga caccgagtcc cggcactcct ggggctcaag cccgttcgag gcgacggcgt
     1861 cgagctcacg agcgagctcg gcggcgagcc ggggcagctg cttcgtcgac acgttggccg
     1921 aagccatcca caggcactcg ctcgcgtcga ggctgaaggc gctgtagacc gagtagcaga
     1981 gcccgagcct ctcgcgcagg gactggaaca gccgcgagct cgaggcctcg ccgagggcgc
     2041 cgttgagcgc cgcgagggcg aggtagtctt tcatcgcgaa gggcggctcg agctgcagcg
     2101 cttcgtagta atgcacctgt tccatgtcgg ctgccagata ggagctgacg ctcctgaagg
     2161 cgggggtcat ctcggcccgc gttgccgcgg cctcggcgga gatcaagacg cccccgagaa
     2221 gggcgccgag ctcgcgctcg acctcgtcca gggggaccgg ccccgccgtc gtcgcgagga
     2281 ggctctcggg gcgcaggctc cggcggtaga actcgtagag cgcgtctcgc gttatgcgac
     2341 gaatgtcttc ggg
//
LOCUS       DASSQV010000336         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_951258, whole genome shotgun sequence.
ACCESSION   DASSQV010000336 DASSQV010000000
VERSION     DASSQV010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951258"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFL04_05845"
     CDS             <1..327
                     /locus_tag="VFL04_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839261.1"
                     /translation="DHIGITFAEPPYSLDLVLQSAVELRIYDCNFPFAVALDGRSATI
                     HAGVNPGRSIDIALTVPADFSARMVAKAGYLEPTAAYRLADGSSPRPGRFLVSASTQI
                     PSGAGQ"
     gene            324..1526
                     /gene="dinB"
                     /locus_tag="VFL04_05850"
     CDS             324..1526
                     /gene="dinB"
                     /locus_tag="VFL04_05850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET7839262.1"
                     /translation="MSRVIFHIDIDAFFASVEQLDDPSLVGRPVIVGASPGHRGVVST
                     CSYEARAFGVHSAMPISEAFRRCPEGVFLPVRMERYAELSARVMEAFGEFTPDLRRVS
                     VDEAFLDMTGTQRLWGPSEAAARLIKARVRAATGLSISIGVAPNPYVAKIASGLRKPD
                     GLVIVEEGEEEAFMLGLPLTKLWGAGEKTQERFRELGIVSVAQLASLGQAQISSLFGR
                     AGGQFLYGAARGRDPGIMGGEPESRSMSAETTFERDIADRESIEAVLMGLADELAYRL
                     WSEGMRSRTLALKLRLHDFTTLSRRLTRKGYYLAAEDAFRDALALLGKAWDGRSEIRL
                     IGLGFAELERDEGQVQGELFADGNERRRRAETAVFEIERKGLGKVTRARLMGRERRGP
                     SGAPGEKP"
     gene            complement(1537..2700)
                     /locus_tag="VFL04_05855"
     CDS             complement(1537..2700)
                     /locus_tag="VFL04_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839263.1"
                     /translation="MDGDMVRRRGPAGAARGLLVSLGLALSFCVAAAQDAPPQALPEM
                     DALLAKAREAIDSNHLAEGIRSYVGVIALFEASAPGQTAGTDEAAAARAKAEAAASEL
                     ARIGARLSIEPASEWLDATGSQVAAATGRVGKADGLSPGLYLYENFGSGKSPVPDAPI
                     HFEFTKNGGSIVPLVSTDAYGKANTTIASLDEPGKEAVVRAYPVFSSRGKTYAFRSVF
                     RDFSFLPAAKTARVLAFASSELGQGGDALTVDSLVSALEPSGLRLSPYNGAMEMEGFR
                     KALAGDGPTLASISSDAASPYVALALVEVGEIRQVELNGRKYNIFSATARAGFRIVRS
                     DGHAVWALPIEGIRAQGGSRESAVADGYRKAREALMAELRKRADEIRDALAKD"
     gene            complement(2682..>2874)
                     /locus_tag="VFL04_05860"
     CDS             complement(2682..>2874)
                     /locus_tag="VFL04_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839264.1"
                     /translation="PEVKTLVDLAFEGYRVALSLRLPSPALSAGIGTAGAGGLQVDYS
                     SPVTALIESPTRIVWTVTW"
BASE COUNT          434 a         1008 c         1005 g          427 t
ORIGIN      
        1 gaccacatcg gcatcacctt cgccgaaccg ccctacagcc ttgacctcgt cctgcagtcg
       61 gcggtcgagc tccgcatcta cgactgcaat ttccccttcg ccgtggccct cgacgggcgg
      121 tcggcgacca tccacgccgg cgtcaacccg ggcaggtcca tcgacatcgc tttgacggtg
      181 cccgccgact tcagcgcgag gatggtggcg aaggccggct acctcgagcc tacggccgcc
      241 taccgcctcg ccgacggctc ctcgccaagg ccggggcgct tcctggtctc ggcctcgacc
      301 cagataccga gcggggccgg acaatgagcc gcgtcatctt ccacatcgac atcgacgcct
      361 tcttcgcctc ggtcgagcag ctggacgacc ccagcctcgt cggaaggccc gtcatcgtgg
      421 gggcgagccc cggccaccgc ggcgtggtct cgacctgctc ctacgaggcg cgggcctttg
      481 gggtccactc cgccatgcca atctcggagg ccttcaggcg ctgcccggag ggcgtcttcc
      541 ttcccgtcag gatggagcgc tacgccgagc tgtccgccag ggtcatggag gccttcgggg
      601 agttcacgcc cgacctgcgc agggtctcgg tcgacgaggc cttcctcgac atgacgggaa
      661 cccagaggct ctgggggccg agcgaggcgg ccgcgcggct catcaaggcc agggtgaggg
      721 cggccacggg gctcagcatc tccatcgggg tcgcgccgaa cccctacgtc gcgaagatcg
      781 cctcgggcct tcgcaagccg gatggcctcg tcatcgtcga ggagggggag gaggaggcct
      841 tcatgctcgg cctgcccctc accaagctct ggggggccgg cgagaagacc caggagaggt
      901 tccgcgagct gggcatagtc agcgtcgcgc aactcgcctc cctcggtcag gcccagatct
      961 cctcgctctt cggcagggcc gggggccagt tcctctacgg cgcggcaagg ggccgcgatc
     1021 ccggcatcat ggggggcgag cccgagagcc gctcgatgag cgccgagacc accttcgagc
     1081 gggacatagc cgaccgggag agcatcgagg cggtgctcat gggcctcgcc gacgagctcg
     1141 cctacaggct ctggtcagag ggcatgcgct cccgcaccct cgcgctcaag ctccgcctcc
     1201 acgacttcac cacgctctcg aggcggctga cccggaaggg ctactacctc gccgccgagg
     1261 acgccttcag ggacgccctc gccctcctcg gcaaggcctg ggacggccgc tcagagatcc
     1321 gcctgatcgg cctcggtttc gccgagctcg agcgcgacga gggccaggtc cagggcgagc
     1381 tcttcgccga cgggaacgag aggaggcggc gggccgagac cgccgtcttc gagatcgagc
     1441 gcaaaggcct gggcaaggtc acgcgggcga ggctcatggg gagggaacgg cggggcccgt
     1501 caggagcgcc cggagagaag ccctaggcgc cctgactcag tccttggcca gggcatccct
     1561 gatctcgtcc gcgcgtttcc gcagctcggc catgagggcc tcgcgggcct tccggtagcc
     1621 gtccgcgacg gctgactccc tgctgccacc ctgggccctg atgccctcga ttggcagggc
     1681 ccagaccgcg tgcccgtcgg agcggacgat gcggaatccc gcccgggccg tggcggagaa
     1741 gatgttgtat ttcctgccgt tgagctcgac ctgcctgatc tccccgacct cgaccagggc
     1801 gagggccacg tagggagagg ccgcatcgga ggatatcgac gcgagggtgg ggccgtcgcc
     1861 agcgagcgcc ttcctgaagc cctccatctc cattgcgccg ttgtaggggc tgagcctgag
     1921 gccgctcggc tccagggccg agacgaggga gtcgaccgtc agggcatcgc ccccctggcc
     1981 gagctcggag ctggcgaagg cgaggacccg cgccgttttc gccgccggca ggaaggagaa
     2041 atcgcggaac acgctccgga aggcgtaggt cttgcccctc gagctgaaga cgggataggc
     2101 gcggacgaca gcctccttcc cgggctcgtc caggctcgcg atggtggtgt tggccttgcc
     2161 gtaggcgtca gtgctcacga ggggaacgat gcttccgccg ttcttcgtga actcgaaatg
     2221 gatgggcgcg tcggggaccg gggacttgcc cgagccgaag ttctcgtaga ggtagagccc
     2281 gggggagagg ccgtcagctt tgccgaccct gcccgtcgcg gccgcgacct ggctgcccgt
     2341 ggcgtcgagc cactcgctcg caggctcgat ggaaagcctg gcgccaatcc tcgcgagctc
     2401 cgatgccgcc gcctcggcct tcgccctggc agcggccgcc tcgtcagtcc cggcagtctg
     2461 gcccggggcc gaggcctcga agagggcgat caccccgacg taggagcgga tgccctcggc
     2521 gagatggttc gagtcgatgg cctcacgggc cttcgcgagg agggcgtcca tctcgggcag
     2581 ggcctgaggc ggcgcatcct gcgcggcggc gacgcagaag gacagggcga ggccaagcga
     2641 gaccaggagg ccccgcgcgg ccccggccgg tccacgccgc ctcaccatgt caccgtccac
     2701 acgatcctcg ttgggctctc gatgagggcc gtcaccggac tcgagtagtc gacctgcaga
     2761 ccgcccgctc ctgccgtccc gatccccgcc gaaagggccg gggaggggag cctgaggctc
     2821 agggcgacgc ggtatccctc gaaggcgagg tcgacgagtg tcttgacctc gggg
//
LOCUS       DASSQV010000337         3678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_702066, whole genome shotgun sequence.
ACCESSION   DASSQV010000337 DASSQV010000000
VERSION     DASSQV010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702066"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1906
                     /locus_tag="VFL04_05865"
     CDS             <1..1906
                     /locus_tag="VFL04_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839265.1"
                     /translation="IPSAFDDSQTASRIMLTLTNVAAGELWLDELLLEDSRGRAALMF
                     QGDLAYSEPELSLGGEALPIVHGLELRADAAGAVQDGSFASGGLFVGTALGPLGVEAQ
                     ARGSAASGSSPTLHGGHALIFPSGGSPLRGEDRFDYNPQTGAFGREDSVSLNAGGLLS
                     LTARQRTAWSPSASSLESGLLDQVWEGRLGAGSELLALSASAANRVRPASWTDLPASY
                     GPAWIAAFRYALPAYQAQSERREEKAGLSLRAGDAGEFASLSMGTVAEPTAAGGADRQ
                     LSAAGKLSLPLDIGGVRVAPYYQRSWRDRRPGSAASLVGDLNTALDDLSSARLAYAGR
                     PLSELSESRTRQEFEDQTAVGSDGLPFAFYAPELGLTLSRDFGSNWYDLLVPSSLSLA
                     RRRELERDASTVSDSAVWDSDLRFGSLNLFGAQGAYPLGLPFDSDEYFASIQASLTKV
                     AGEAAERIKIQAQHLATFYAGEADRLDAENRISVSTQPGSSAWDERIRLSVSRRQTRH
                     WLLDLYRMAVPGATAAAPSTAASSAAAPGAAADQGVSLVSLYLADLQGRSPNLRSIVE
                     LDASLSRTVTDAAKPGLTWGLAESYEAKLTVPERLTVSVKGALSQGEDGTTSVVEVDL
                     TLSLAVTISF"
     gene            1973..>3678
                     /gene="metG"
                     /locus_tag="VFL04_05870"
     CDS             1973..>3678
                     /gene="metG"
                     /locus_tag="VFL04_05870"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7839266.1"
                     /translation="MKRRLITSALPYVNNVPHLGNLIQVLSADVFARFCRLRGYETLY
                     VCGTDEYGTATETKALEEGVSPRELCDRFHALHRDIYSWFGIAFDKFGRTSTERQTEI
                     TQSIFLDLDRNGLIKQQTIEQLFCENDQRFLADRYVKGICPACGYDGARGDQCENCGK
                     LLDPTELKNPVCSTCGATPKPRSTTHLYIDLPAIRPKLEAWMKEASVRGFWANNAVQM
                     TQAWIRDGLKERAITRDLKWGIPVPKEGFENKVFYVWFDAPIGYISITACAGDEQGFD
                     WKSWWMSPEDTELFQFIGKDNIPFHTVIFPSSLLGSGRKWTMLHHMSSTEYLNYEAGK
                     FSKSKGVGVFGTDAVESGIPADVWRFYIFFNRPEKSDTTFTWSDFQEKVNGELIGNLG
                     NLANRTLTFVSRYYGGAIPEGKSDEAFWKTAREIEARIAGHLDRAELRDAFRAAFELS
                     DIANKRFQDGEPWKTRTSDPEKAASLIRDLCFLLRDLAVIIHPYMPHAADRLASFFGR
                     SFGQGGLSWAEVGQLSGIDRVLKTEVLFAKLEDELIAGLRERFSGSQAERQVRDEKAA
                     AAG"
BASE COUNT          600 a         1340 c         1178 g          560 t
ORIGIN      
        1 catcccatcg gccttcgacg actcccagac ggcctcgagg atcatgctta ccctgacgaa
       61 cgtcgccgcg ggcgagctct ggctcgacga gctcctcctc gaggactcga gggggagggc
      121 agccctcatg ttccagggcg acctcgccta ctcggagccc gagctctcgc tcggaggcga
      181 ggccctcccc atcgtccacg gcctcgagct ccgggccgac gccgcggggg cggtgcagga
      241 cggttccttc gcctcgggcg ggctcttcgt cggcaccgcc ctgggaccct tgggagtcga
      301 ggcccaggct cggggctcgg cggcgagcgg ttcgagtccg accctccacg gcgggcacgc
      361 ccttatcttc ccctccggcg gctcgcccct gcgcggcgag gacaggttcg actacaatcc
      421 ccagacgggc gccttcggca gggaggattc ggtttcgctc aacgcgggag gcctcctgtc
      481 cctgacggcg aggcagcgca cggcctggag cccctcggcc tcgagcctgg agtccggcct
      541 cctcgaccag gtctgggagg gccgccttgg ggcgggctct gagctactgg ccctgagcgc
      601 ctcggcggcc aatcgggtga ggccggcctc ctggaccgat ctccccgcct cctacggccc
      661 ggcctggatc gccgcattca ggtacgcgct cccggcctac caggcccagt ccgaaaggcg
      721 cgaggagaag gccgggctca gcctccgcgc cggagacgcc ggggagttcg cctcgctctc
      781 catggggacg gtcgccgagc ccacggccgc gggcggtgcc gaccgccagc tgtccgcggc
      841 cgggaagctc agccttcccc tggacatcgg gggcgtcagg gtcgcgccct actaccagcg
      901 ctcgtggagg gacaggaggc cgggcagcgc ggcctccctt gtcggcgacc tgaacacggc
      961 cctggacgac ctctcctcgg ccagactggc ctacgcgggc aggccactgt cggaactctc
     1021 ggaatcgagg acgaggcagg agttcgagga ccagacggcc gtggggagcg atggcctgcc
     1081 tttcgccttc tacgcgcccg agctgggcct cacgctctcg cgcgacttcg gctccaattg
     1141 gtacgacctc ctcgtgccct cgtccctgag cctggcgcgg aggcgggagc tcgagcgcga
     1201 cgcctcgacc gtcagcgact cggccgtatg ggactcggac ctgaggttcg gctccctgaa
     1261 cctgttcgga gcccaagggg cctatcctct gggccttccc ttcgacagcg acgagtattt
     1321 cgcctcgatc caggccagcc tcacgaaggt ggcgggggag gcggccgagc ggatcaagat
     1381 ccaggcccag cacctggcga ccttctacgc cggcgaggcc gaccgcctcg acgctgagaa
     1441 caggatatcg gtctcgaccc agcccggatc atcagcctgg gacgagagga tccgcctctc
     1501 ggtctcgaga cggcagaccc ggcattggct cctcgacctc taccggatgg ccgtcccggg
     1561 cgccacggcc gctgcgccgt ccacggcggc gtcgtccgcg gcggcgccgg gcgcggcggc
     1621 ggaccaggga gtctccctcg tctcgctcta cctcgccgac ctccagggcc gcagccccaa
     1681 tctgcggtcc atcgtcgagc tggacgcctc cctctccagg acggtcacgg acgccgcgaa
     1741 gccggggctg acttggggcc tggcggagag ctacgaggcc aagctcacgg tgccggaacg
     1801 gctcacggtg agcgtgaagg gggccctgag ccagggagag gacgggacga cgagtgtcgt
     1861 ggaggtcgac ctcaccctga gcctggccgt caccatctcc ttctgagcgt cggagggccc
     1921 cgagggacga atccttgaca ccccgccggg gccgcgttta ttgttcgagg ctatgaaacg
     1981 acgactcatc acctcggcgc ttccttatgt gaacaacgtg ccgcaccttg ggaacctgat
     2041 ccaggtcctc tcggccgacg tcttcgcccg cttctgccgc ctccgtggct atgagaccct
     2101 ctacgtctgc ggcaccgacg agtacggcac ggcgaccgag accaaggccc tcgaggaggg
     2161 cgtgagcccc cgtgagctct gcgaccgctt ccacgccctc caccgcgaca tctactcctg
     2221 gttcgggatc gccttcgaca agttcgggcg cacctccacc gagcgccaga ccgagataac
     2281 ccagtccatc ttcctcgacc tcgaccgcaa cggcctcatc aagcagcaga ccatcgagca
     2341 gctcttctgc gagaacgacc agcgcttcct cgccgaccgc tacgtgaagg gcatctgccc
     2401 cgcctgcggc tacgacgggg cgcgaggcga ccagtgcgag aactgcggca agctcctgga
     2461 cccgaccgag ctcaagaacc cggtgtgctc gacctgcggg gccaccccca agccgcgcag
     2521 caccacccac ctgtacatag acctgcccgc catccgccca aagctcgagg cctggatgaa
     2581 ggaggcgagc gtacgcggct tctgggccaa caacgcggtc cagatgaccc aggcctggat
     2641 ccgcgacggc ctcaaggagc gcgccatcac gcgcgacctg aagtggggca tccccgtgcc
     2701 caaggaaggc ttcgagaaca aggtcttcta cgtctggttc gacgcgccca tcggctacat
     2761 ctcgatcaca gcctgcgccg gggacgagca gggcttcgac tggaagtcct ggtggatgtc
     2821 gcccgaggac accgagctct tccagttcat cggcaaggac aacatcccct tccacacggt
     2881 catcttcccc tcgagcctcc tcggttcggg caggaagtgg accatgctcc accacatgtc
     2941 gagcaccgag tacctcaact acgaggccgg gaaattctcg aagtcgaagg gtgtgggggt
     3001 cttcggcacc gacgccgtgg agtctggcat ccccgccgac gtctggcgct tctacatctt
     3061 cttcaaccgc ccggagaaga gcgacacgac cttcacctgg tccgacttcc aggagaaggt
     3121 gaacggcgag ctcatcggca acctcggcaa cctcgccaac cgcaccctca ccttcgtgag
     3181 ccgctactac ggcggcgcca tccccgaggg caaaagcgac gaggccttct ggaagacggc
     3241 gagggagatc gaggcccgca tcgccgggca cctggacagg gccgagctcc gcgacgcctt
     3301 ccgcgccgcc ttcgagctct ccgacatcgc caacaagcgc ttccaggacg gcgagccctg
     3361 gaagacgagg acgagcgacc cagaaaaggc cgcctcgctc atacgcgacc tctgcttcct
     3421 cctccgcgac ctcgccgtca tcatccatcc ctacatgccc catgcggccg acaggctcgc
     3481 ctccttcttc ggcaggagct tcggccaagg cggcctgtcc tgggccgagg tcgggcagct
     3541 ctcgggcatc gacagggtgc tgaagaccga ggtcctcttc gccaagctcg aggacgagct
     3601 catcgccggc ctgcgcgagc gcttctcggg ctcccaggcc gaacgccagg tcagggatga
     3661 gaaggccgcc gcggctgg
//
LOCUS       DASSQV010000338         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2070581, whole genome shotgun sequence.
ACCESSION   DASSQV010000338 DASSQV010000000
VERSION     DASSQV010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070581"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFL04_05875"
     CDS             complement(<1..430)
                     /locus_tag="VFL04_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7839267.1"
                     /translation="MHRRSNLLQRASLIALFGNALLAVSKLVVGFLSGSLAVVGDGID
                     TSTDVVIAFVALLTSRIIDKPGDSEHPYGHTRAETVATTILSFLVFFAGAQLFARSAG
                     ALLSGEAAVLPGRVAIIVTVVSIAGKLLLAWSQFHYGKKTG"
     gene            597..1256
                     /locus_tag="VFL04_05880"
     CDS             597..1256
                     /locus_tag="VFL04_05880"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7839268.1"
                     /translation="MIAALYEDEEILVVDKPSGLASQPGAEVRVSVVEAVERDYGCRP
                     FLVHRLDKETSGCMIVAKSAAAASRWAGLVEARSLRKVYRAVAAGRPEGEKGQLSDPV
                     RERGVEKSAHTDWRLLGAFAGLEGGPLLSYLELELGTGRMHQIRIHLAGAGTPILGDE
                     RHGDFPLNRRLRKEAGLRRLLLHARSLVLPGARVVTAPAPEHLRDFLARFPGAPDPEC
                     P"
     gene            1253..1636
                     /locus_tag="VFL04_05885"
     CDS             1253..1636
                     /locus_tag="VFL04_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839269.1"
                     /translation="MKATALPPYALVHGASWLTPEGEVIPIPSFHEEWIREHEELAEG
                     SRNVCELILRKRWISVALFSEGYLELMVPSKRDEATKAMVTELLTRNRGAWTKALVMS
                     MDEEGYAMLSPGDLDRLSERLSQGI"
     gene            complement(1633..2424)
                     /locus_tag="VFL04_05890"
     CDS             complement(1633..2424)
                     /locus_tag="VFL04_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7839270.1"
                     /translation="MREDGVGQTLVGTCGYSYDEWRGVLYPPELPKAEFLPFYAGTFP
                     FVELDFSWYGMPKASTLARMVGLTPGAFQFAIKAHRSMSHDRGPDWRERAREFATAIE
                     PLATAGRLATILIQLPQSFRRGEEERRYLASLCDELAAFPLAVEFRNGEWHRESVFAE
                     LDRRGAALVQVDRPDLPGLPPESPVVTGGRGYIRFHGRNKDAWWAGGAGGRYDYLYSE
                     EELGSRLPLIREVQRKTEVLIVAFNNHPGGSAVVNARSMALLLES"
     gene            complement(2421..>2662)
                     /locus_tag="VFL04_05895"
     CDS             complement(2421..>2662)
                     /locus_tag="VFL04_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7839271.1"
                     /translation="VEKVFRMYPDPQATDQDVVLVDEKIDSFLEKHFDQYRLYCSKKR
                     PHLDPQHENPGYLLYAGIKEDDQYDDLTILGIMRK"
BASE COUNT          406 a          889 c          945 g          422 t
ORIGIN      
        1 tgcccgtctt cttgccgtag tggaactggc tccaggcgag gaggagcttg cccgcgatgg
       61 agaccaccgt gacgatgatg gcgacgcggc ccggcaggac ggccgcctcc ccgctcagga
      121 gggcccccgc cgaacgggcg aagagctggg ctccggcgaa gaagacgagg aaggagagga
      181 tcgtggtggc cacggtctcg gcccgcgtgt ggccgtaggg gtgctcgctg tccccgggct
      241 tgtcgatgat gcgcgaggtc aggagggcga cgaaggcgat cacgacgtcg gttgaggtgt
      301 cgatgccgtc gcccacgacg gcgagggagc cggagaggaa gcccacgacc agcttcgaca
      361 ccgcgagcag ggcgttgccg aagagtgcga tgagggatgc tcgctggagc aggttgctgc
      421 gtcggtgcat ggcttgtcct ccgggaggat gaggctagca cgggccgaaa gcccatctca
      481 agcggagctg caataatgtg aggcttgtct aactgcgggc tttggtcgcg gggggcggca
      541 aaagcccggg catgcggcct tgcgcagagg gatgggctcc actatgctct ggcgcgatga
      601 tcgccgccct ctacgaggac gaggaaatcc tcgtcgtcga caagccctct ggcctcgcct
      661 cccagccggg ggccgaggtc cgcgtctcgg tggtggaggc agtcgagcgc gactacggct
      721 gcaggccctt cctggtccac cgcctggaca aggagacctc gggctgcatg atcgtcgcaa
      781 aaagcgcggc cgccgcctcg cgctgggccg gcctcgtcga ggctcgctcg ctcaggaagg
      841 tctaccgcgc cgtggcggcc gggcgccccg agggcgagaa gggccagctc agcgaccctg
      901 tccgggagcg gggggtggag aaaagcgccc acaccgactg gcgcctcctc ggggccttcg
      961 ccggcctcga gggcgggccc ctgctctcct acctcgagct cgagctgggc acggggagga
     1021 tgcaccagat ccgcatccac ctcgcggggg ccgggacccc gatcctcggg gacgagcgcc
     1081 acggcgactt tccgctgaac cggcggctcc gcaaggaggc cgggctcagg cgcctcctcc
     1141 tccacgcgag gagccttgtc ctgcccgggg ctagggtcgt gacggctcca gcccccgagc
     1201 acctgcggga ctttctcgcc cgcttccctg gggcgcccga cccggagtgc ccatgaaggc
     1261 cacggccctg cctccctacg ccctcgtcca tggcgcgagc tggctgaccc ccgagggcga
     1321 ggtcataccc atcccctcct tccacgagga gtggatccgc gagcacgagg agctggccga
     1381 aggctcacgg aacgtgtgcg agctcatcct ccgcaagcgc tggatctcgg tcgccctctt
     1441 cagcgagggc tatctcgagc tcatggtccc ctccaagcgc gacgaggcca cgaaggcgat
     1501 ggtgaccgag ctcctgaccc gcaaccgcgg cgcctggacc aaggccctcg tgatgtcgat
     1561 ggacgaggaa ggttatgcga tgctgagccc cggggacctc gaccgcctgt ccgagcgcct
     1621 ctctcagggc atctaggact cgagcagcag ggccatgctc cgggcgttca ccacggcgct
     1681 gccccccggg tggttgttga aggccacgat gaggacctcg gtcttcctct gcacttccct
     1741 gatgaggggc aggcgcgagc cgagttcctc ctcgctgtac aggtagtcgt agcgcccccc
     1801 ggctccccct gcccaccacg cgtccttgtt ccgtccgtgg aagcggatgt atcccctccc
     1861 gcccgtcacc accgggctct ccggcggcag accgggcagg tcggggcggt cgacctggac
     1921 aagggccgcg ccccgacggt cgagctcggc gaagacgctc tcgcggtgcc actcgccgtt
     1981 gcggaattcc acggccaggg ggaaggccgc cagctcgtcg cagagcgagg cgaggtatcg
     2041 cctctcctcc tcgccccgcc tgaagctctg gggaagctgg atgaggatcg tggcgaggcg
     2101 gccggccgtg gccaggggct cgatggccgt ggcgaactcg cgggccctct ccctccagtc
     2161 agggccgcgg tcgtggctca tgctgcggtg ggccttgatc gcgaactgga aagccccggg
     2221 cgtgagcccc accatgcgcg cgagggtcga ggccttgggc atgccgtacc agctgaagtc
     2281 gagctcgacg aaggggaagg tcccggcgta gaaggggagg aactcggcct tcggcagctc
     2341 gggcggatag aggaccccgc gccactcgtc gtaggagtag ccgcaggttc cgaccagggt
     2401 ctggcccact ccatcctccc tcatttcctc atgatgccca ggatcgtcag gtcgtcgtac
     2461 tggtcgtcct ccttgattcc cgcgtagagg aggtagccgg ggttctcgtg ctgggggtcg
     2521 aggtggggcc gcttcttcga gcagtagagc cggtactggt cgaagtgctt ctccaggaag
     2581 ctgtcgatct tctcgtccac gaggacgacg tcctggtcgg tggcctgggg gtcggggtac
     2641 atgcggaaga ccttctccac cg
//
LOCUS       DASSQV010000339         5338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_702566, whole genome shotgun sequence.
ACCESSION   DASSQV010000339 DASSQV010000000
VERSION     DASSQV010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702566"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VFL04_05900"
     CDS             <1..566
                     /locus_tag="VFL04_05900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519705.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET7839272.1"
                     /translation="GYPGYLYSDLASLYERAGRIEGSSGSITQIPILSMPNDDVTNPI
                     PDLTGYITEGQIVVDRELSQRGLYPPIAVLPSLSRLMKDGIGAGMTRDDHKEVSSQLF
                     AAYARVKSVRNLAAIIGEEELSDLDKTYLRFGERFEAEFVGQGEDSDRSIAETLDIGW
                     GLLSILPRGELLRVSKAMIEKYLPKAE"
     gene            568..1200
                     /locus_tag="VFL04_05905"
     CDS             568..1200
                     /locus_tag="VFL04_05905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HET7839273.1"
                     /translation="MAGPNVAPTKSNLIREKERLALAIEGYELLERKREILMMELMKR
                     VEELRVLERELDASAGRAYPAMRLMLLRTGREGARDLAAGVEPEIAVRERTVQVAGLH
                     VPSVEAEALPRRLQTSFMDSSALSDETVLEFTELLWLAARMAGLRSVVWRLAKEVRKT
                     QRRVNALEKMVIPESRARKAFIEASLEEREREAIFAVKLLKARRAEKGEG"
     gene            1197..1679
                     /locus_tag="VFL04_05910"
     CDS             1197..1679
                     /locus_tag="VFL04_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7839274.1"
                     /translation="MKGLLSNIVVVVNGAESSIAAVKYAISLQKAYGCRVTAVYVVDT
                     HTIRQLAHARIFIAEESAEYERELESTGRRYLAFVEELARTRGIAVETRLVSGSIASE
                     VVRCADELEADCILLGSFERDTVFRDVIVEASREILRFAHCSVLIVRARDAERAFRPE
                     "
     gene            1676..2107
                     /locus_tag="VFL04_05915"
     CDS             1676..2107
                     /locus_tag="VFL04_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839275.1"
                     /translation="MSAHQARVAVVYLPAGDPGPLNAMARAMVGALEAAGHRAECFEA
                     GGHGSPRLTGYDYVILGTEGRGFGGRIPPRLRGFLAQAGTVSGKRSMAFVRKTGLSPA
                     KALKRLMAAMESEGMLVNCAEIVAGGEDAAAAVRAAPVERK"
     gene            2111..2938
                     /locus_tag="VFL04_05920"
     CDS             2111..2938
                     /locus_tag="VFL04_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET7839276.1"
                     /translation="MENYYEVLGVHPEAPSQAVKSAFRKQAKKHHPDLSAGRPSETGA
                     SARAADAAMRLLLEAYRILSDPEKRRAYDRSLRRRAAEEGGFDYRKFLKARPDDPESQ
                     AKLVVYDLLHELEEEAIEVYERAKTLGGEFRLERWLERGEAMDAEYCIAEEYESRERY
                     LKAYALYKRLIQMELEKPWFRYFFDVVALRFRTLLLFKLPKILDEEDFIDRLEEAAAL
                     GISRRDSAQFLRKKAEVHLRRHETEAAVAALRRAGELEPRLAGLSALRRRAGIQTGA"
     gene            3056..4099
                     /locus_tag="VFL04_05925"
     CDS             3056..4099
                     /locus_tag="VFL04_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET7839277.1"
                     /translation="MKRNKSLVSIAATIALLLPLSAAGAQTIDKPAATVKLIKLEVYS
                     VRQFKADVDKVEAAIGRKLDPATRRQMLDSQLNALLFKQYCDREKILVTDADVNSGIA
                     EMKQQVGPNADDAQLEAAMRSQGIFNDPKTYVRQQLLLSRFLQAKKADELKALKSPTP
                     DEILKAYDLQKSALVRPDTDRVSILYIDLRGKSDAEKKKSSDTFRAVVAQLKSNSAKF
                     DELMLKAADPQAGYKATVSLMVEKTPQAQNLYGNEFFDAVFKLKVGEISPLIENAAGL
                     QVVRLNEALPQKQLTLSDPVPGQANATVQDYIAYNLTMKTRTEFLQKVQDDLLAQLRK
                     EATIKIFDENLNF"
     gene            4123..4545
                     /gene="nusB"
                     /locus_tag="VFL04_05930"
     CDS             4123..4545
                     /gene="nusB"
                     /locus_tag="VFL04_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET7839278.1"
                     /translation="MASRRKARILAFQALYAWDAGGGAEWDLLSFPWLEEDKLSSLDD
                     EVASFARLLIAGTLEKIGSVDEAIRSHLQNWAFERLKRVDLAVLRIGAYSLLFQADIP
                     AQITIDEAIEIVKEYGSEDSYRFVNGVLDGIWKSIAAR"
     gene            4514..>5338
                     /locus_tag="VFL04_05935"
     CDS             4514..>5338
                     /locus_tag="VFL04_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839279.1"
                     /translation="MASGSRSPHASRASLAAACLLALATALSCSLLRPPDFPKALAGL
                     DAALSASVRGRALDSAFEAAYRSAGSSAEWLSVLKRAREVELAGDLRRYARAAERALA
                     AGGRSEALAAAAAHGLLRGGEPGKAYALFKTRLSPDSRPGLWAEAVLAIAREGKLAEG
                     SLGAADFDRLAETAGESRFYLNAAVLHLAEGDVQASGNSVARAMAAGLDPGAELLWDA
                     GRYQALAARRGPLPGSGAKAGSSELALIGDASWVLGDAGSARRAWQRSVALDPRSSW"
BASE COUNT          946 a         1845 c         1765 g          782 t
ORIGIN      
        1 agggctaccc gggctacctc tattccgacc tcgcctcgct ctacgagcgg gcggggcgca
       61 tcgagggctc gagcggctcg atcacccaga tccccatcct ctcgatgccg aacgacgatg
      121 tcacgaaccc catccccgac ctcaccggct acatcaccga gggccagatc gtcgtcgaca
      181 gggagctgtc ccagaggggc ctgtatccgc ccatcgccgt cctgccctcc ctgtccaggc
      241 tcatgaagga cggcatcggg gccgggatga cccgggacga ccacaaggag gtctcgagcc
      301 agctcttcgc ggcctatgcc agggtcaaga gcgtgcgcaa cctcgccgcc atcatcggcg
      361 aggaggagct ttccgacctc gacaagacct atctccgctt cggcgagcgt ttcgaggccg
      421 agttcgtcgg ccagggcgag gacagcgacc ggagcatcgc tgagaccctc gacatcggct
      481 ggggccttct gtcgatcctc ccccgcgggg agctcctccg ggtctcgaag gccatgatcg
      541 agaaatacct cccgaaggcg gaataggatg gcggggccga acgtcgcgcc gaccaagtcg
      601 aacctcatcc gcgagaagga gaggctcgcc ctcgccatcg agggctacga gctgcttgag
      661 cgcaaacgcg agatcctcat gatggaactc atgaagcgcg tggaggagct gagggtcctg
      721 gagcgggagc tggacgcgag cgcgggcagg gcctaccccg cgatgcggct catgctcctg
      781 cggacgggca gggagggggc gagggacctg gccgccggcg tggagcccga aatcgcggtg
      841 agggagagga cggtccaggt ggccggcctc catgtgcctt cggtcgaggc cgaggccctg
      901 ccccgccgcc tccagacctc cttcatggat tcctcggcac tttccgacga aacggtgcta
      961 gagttcaccg agctcctctg gctcgccgcc aggatggccg gtctccggtc cgtcgtctgg
     1021 cggctcgcga aggaagtgag gaagacccag aggagggtca acgcgctgga gaagatggtc
     1081 ataccggagt cgcgggcgcg caaagccttc atcgaggcct ccctcgagga gcgcgagcgc
     1141 gaggccatct tcgctgtgaa gctcctgaag gctcggagag ccgagaaggg tgaaggatga
     1201 aaggtctgct ttcgaacatc gtggtcgtcg tcaacggggc cgagtcctcg atcgcggcgg
     1261 tcaagtacgc gatctcgctc cagaaggcct acgggtgcag ggtcacggca gtctatgtcg
     1321 tcgacaccca caccatcaga cagctggcgc acgcccgcat cttcatcgcc gaggagagcg
     1381 ccgagtacga gcgcgagctc gagtcgacgg gcaggcgcta cctggccttc gtcgaggagc
     1441 tcgctcggac caggggcatc gcggtcgaga cccggctcgt aagcggctcc atcgcctccg
     1501 aggtggtcag atgcgcggac gagctcgagg ccgactgcat cctcctcggc agcttcgagc
     1561 gggacacggt gttccgggac gtcatcgtgg aggcctcgcg ggagatcctc cgtttcgccc
     1621 actgctcggt cctcatcgtg agggccaggg acgccgagcg ggcgttcaga cccgaatgag
     1681 cgcccatcag gcgagggtag ccgtggtcta ccttccggcc ggggatcccg gcccccttaa
     1741 cgcgatggcg agggccatgg tcggcgccct cgaggcggcc ggccacaggg cggaatgctt
     1801 cgaggcgggg ggccatgggt cccctcggct aactggatac gactatgtta tcttagggac
     1861 cgagggaagg ggtttcggcg ggaggatccc gcccaggctc cgcggcttcc tcgcccaggc
     1921 cggaacggtc tcggggaagc ggagcatggc cttcgtgagg aaaacgggcc tctcccccgc
     1981 aaaggccctc aagcgcctga tggcggccat ggagtcggaa ggaatgctgg tgaactgtgc
     2041 agagatcgtg gccggcggcg aggacgccgc ggccgccgtg agggccgccc cggtcgaacg
     2101 gaaatagcga gtggagaatt actacgaagt cctcggtgtc catccagagg cgccttccca
     2161 ggcggtaaag agcgcattca ggaagcaggc gaagaagcac caccccgacc tgtcggctgg
     2221 gaggcccagc gagaccgggg cctcggcccg ggcggcggac gcggccatgc gcctcctcct
     2281 cgaggcctac cgtatcctct ccgatccgga gaagcgccgg gcctacgacc ggagcctcag
     2341 gaggcgggcc gccgaggaag gcggcttcga ctaccgcaag ttcctgaagg ccaggcccga
     2401 cgaccccgag agccaggcca agctcgtcgt gtacgacctc ctccacgagc tcgaggagga
     2461 ggcgatcgag gtctacgagc gcgccaagac cctgggcggg gagttcaggc tggagcgctg
     2521 gctcgagcgg ggcgaggcga tggacgccga atactgcatc gccgaggagt acgagagccg
     2581 cgagcgctac ctcaaggcct atgccctgta caagcggctg atccagatgg agctcgagaa
     2641 gccctggttc cgctacttct tcgacgtcgt cgccctccgg ttcaggaccc tcctgctctt
     2701 caagctgccg aagatcctcg acgaggagga cttcatcgac cgcctggagg aggccgccgc
     2761 cctcggcatc tcccgccggg actcggccca gttcctcagg aagaaggccg aggtccatct
     2821 gcggcggcac gagaccgagg cggcagtcgc ggccctccgg agggcggggg agctcgagcc
     2881 gaggctcgcg gggctgagcg ccctcaggcg gcgggctggc atccagaccg gggcctagaa
     2941 ggactccatt gcgcaaagca gggatttcgt ttacctttcc ccgaagatta cgcagcataa
     3001 gcggggcgct ggcgatgctt catcgcccgg aagccacagc ccgggaagga ttcgtatgaa
     3061 acggaacaag agcctggtat cgatcgcagc gacaattgcc ctcctcctgc cgctttcggc
     3121 ggccggggcc cagaccatcg acaagcccgc ggcgaccgtg aagctcatca agctcgaggt
     3181 ctattcggtc aggcagttca aggccgacgt cgacaaggtc gaggcggcca tcggccgcaa
     3241 gctcgatccc gccacccgcc gccagatgct cgacagccag ctcaacgccc tccttttcaa
     3301 gcagtactgc gaccgcgaga agatcctggt gaccgacgcc gacgtcaact ccggcatcgc
     3361 cgagatgaag cagcaggtcg gtccgaacgc cgacgacgcc cagctcgagg ccgcgatgcg
     3421 cagccagggc atcttcaacg atcccaagac ctatgtgcgc cagcagctcc tcctgagccg
     3481 cttcctccag gccaagaagg ccgacgagct caaggccctg aagagcccca cgcccgacga
     3541 gatcctcaag gcttatgacc tccagaagtc ggccctcgtc aggccggaca ccgacagggt
     3601 gagcatcctc tacatcgacc tgcgcggcaa aagcgacgcc gagaagaaga agagctccga
     3661 caccttccgc gccgtcgtgg cccagctcaa gagcaacagc gccaagttcg acgagctcat
     3721 gctcaaggcc gccgaccccc aggccggcta caaggccacc gtctccctca tggtcgagaa
     3781 gaccccccag gcccagaacc tctacggcaa cgagttcttc gacgccgtct tcaagctcaa
     3841 ggtgggcgag atcagccccc tgatcgagaa cgccgccggc ctccaggtcg tccgcctgaa
     3901 cgaggccctg ccccagaagc agctgaccct ctccgatccc gtgccgggcc aggccaacgc
     3961 cacggtccag gactacatcg cctacaacct caccatgaag acgaggacgg agttcctcca
     4021 gaaggtccag gacgatctcc tcgcccagct ccgcaaggaa gccacgatca agatcttcga
     4081 cgagaacctc aacttctgag cctgcgatag ggagaaagcc cggtggcatc gcgaagaaaa
     4141 gcccggatcc tcgccttcca ggccctctac gcctgggacg cggggggcgg ggcggaatgg
     4201 gacctcctct ccttcccctg gctcgaggag gacaagctca gctcgctcga cgatgaggtc
     4261 gcctccttcg cgaggctcct catcgccggc accctcgaga agatcgggag cgtcgacgag
     4321 gccatccgct cccatctgca gaactgggcc ttcgagcgcc tcaagcgcgt cgacctcgcc
     4381 gtgctccgca tcggggccta ctccctcctc ttccaggccg acatcccggc ccagatcacc
     4441 atcgacgaag cgatcgaaat cgtgaaggaa tacggttccg aggattcata ccgtttcgtg
     4501 aacggggtgc tcgatggcat ctggaagtcg atcgccgcac gctagccgcg cctccctggc
     4561 cgccgcgtgc ctcctcgccc tcgccacagc cctctcctgc tccctcctca ggccgcccga
     4621 cttccccaag gccctcgccg gccttgacgc ggcgctctcg gcctcggtcc gggggcgggc
     4681 cctcgactcg gccttcgagg cggcctaccg ctcagccggg agcagcgccg aatggctctc
     4741 ggtcctgaag cgggcgcgcg aggtcgagct ggcgggagac ctcaggcgct acgcccgcgc
     4801 ggccgagcgg gccctcgccg ccgggggccg ctccgaggcc cttgccgcgg ccgccgccca
     4861 cggcctcctc cgcgggggcg agcctggcaa ggcctacgcc ctcttcaaga cgaggctctc
     4921 gcccgactcg aggccggggc tctgggccga ggcggtcctg gccatagcga gggaagggaa
     4981 gctggccgag ggtagcctcg gcgccgccga tttcgaccgc ctcgccgaga ccgcgggcga
     5041 gagccgcttc tacctcaacg ccgccgtgct ccatctcgcc gagggcgatg tccaggcctc
     5101 gggcaattcg gtggcgcggg cgatggccgc tggcctcgac ccgggggccg agctgctctg
     5161 ggatgctggc aggtaccagg ccctcgccgc ccgccgcggg cccctgccag gctcgggagc
     5221 caaggcgggc tccagcgagc tggccctgat cggcgacgcc tcctgggtcc tcggggacgc
     5281 tggctcggcg cgccgggcct ggcagcgctc ggtggccctc gatccgcgct cctcgtgg
//
LOCUS       DASSQV010000340         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1573001, whole genome shotgun sequence.
ACCESSION   DASSQV010000340 DASSQV010000000
VERSION     DASSQV010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573001"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1053)
                     /locus_tag="VFL04_05940"
     CDS             complement(40..1053)
                     /locus_tag="VFL04_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET7839280.1"
                     /translation="MNETDPRYSGIGLVADMRQVPAIVRGLDTARIIAFAERIRGEAV
                     LLSGEGSSRIFPAGKVLADSRRHSYRDRMYTEACTQALEYELASTTVFVASNSGRTKE
                     GVRLIRWLREKGCAGIIGVVAQAGTPIALEADASYILSCGPERAIAASKSVVEQALFY
                     DLLFRSRNGLALPDLGLLAERMDAALRLTVPRETTKALAAASMLYWAGRKDGVAEELT
                     LKTNEMTRKKSDYLEGTYAAHGIEEVMDASDAAIIVDPFPAEEQKFEEVLAGTVGLKT
                     FAIAHRASRFPSLQVEGAGDMTPYVQLCAGWNLLVEVGIALGVNLDKPVRARKVGNEF
                     LGA"
     gene            complement(1058..2260)
                     /locus_tag="VFL04_05945"
     CDS             complement(1058..2260)
                     /locus_tag="VFL04_05945"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HET7839281.1"
                     /translation="MKGNAIVGQSGGPTAVINASLAGVVRGCRADPRIGRVFGMRFGI
                     EGFMEDLLVDLGALGGEALDLLARTPSSALGSCRHKLAEADFPRILEALKRHDIRYFF
                     LIGGNDTMDTIHRVEAWSRAHGWELRGIGVPKTVDNDLFGTDHTPGYPSAARYVALSV
                     RQAGRLAKDMRRVDSFVVHQTVGRDSGWLAAASALARSRPGEAPHLIYLPERPITREA
                     LLSEVRACISQWGWCSIVCGEGLLWEDGSPVSATSSRDRFSNVEFGAMGGGSAALAIH
                     GLLREATGLRGEFQVTESLPMCADDRVSGLDLEEAMACGLRAAELALGGASGLMVTIQ
                     REAGPAYRASYGTAPLSEVAVRAKPMPEAFLAPGRPDVSPAFLDYLRPLVGDLPQYFD
                     IESLASKG"
     gene            complement(2434..>2804)
                     /locus_tag="VFL04_05950"
     CDS             complement(2434..>2804)
                     /locus_tag="VFL04_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839282.1"
                     /translation="AEAGCLWAADAAEFLVGKAVPFRTAYAAGRALVEAWARAAGPGG
                     GFGRNPSVAGLIASLGEPGLAALHTAWSGVQPGELAPYLSLEACVARRDLVGGPAPAR
                     CAAEIGRLLSFVERERLGLG"
BASE COUNT          402 a          962 c         1047 g          393 t
ORIGIN      
        1 cctgcccccg atggaactgc gggccacgcg gtacacgctc taggccccca ggaactcgtt
       61 gcccaccttg cgcgccctga cgggcttgtc cagattgacg ccaagggcta tgccgacctc
      121 gacgaggagg ttccagccgg cgcagagctg gacgtagggc gtcatgtctc cggcgccctc
      181 gacctggagg gaggggaacc tggacgcgcg atgcgcgatc gcgaatgtct tgaggcccac
      241 ggtgccggca aggacctcct cgaacttctg ctcctcggcg gggaaggggt cgacgatgat
      301 ggccgcgtcg gaggcatcca tgacctcctc gatgccgtgg gccgcgtagg tgccctcgag
      361 gtagtcggac ttcttgcgcg tcatctcgtt ggtcttgagg gtgagctcct cggccacccc
      421 gtccttcctc cccgcccagt agagcatgga agcggcggcc agggccttgg tggtctcgcg
      481 tgggacggta agccggaggg cagcgtccat gcgctcggcc agaaggccga ggtcgggaag
      541 ggcgaggcca ttgcgcgagc ggaagaggag gtcgtagaag agggcctgct ccaccacgct
      601 cttcgaggcc gcgatcgcgc gctcggggcc gcaggagagg atgtaggagg cgtcggcctc
      661 cagggctatg ggcgtgcccg cctgggccac gacgccgatg atgccggcgc agcccttctc
      721 ccgcagccag cggatgaggc ggacgccctc cttggtcctg cccgagttcg aggcgacgaa
      781 gaccgtcgtc gaagcgagct cgtactcaag ggcctgggtg caggcctcgg tgtacatcct
      841 gtcccggtag gagtgcctgc gcgagtcggc gaggaccttg cccgccggga agatccggga
      901 ggagccctct cccgagagga ggacggcctc gcccctgatc cgctcggcga aggcgatgat
      961 ccgggccgtg tcgagccccc gtacgatggc ggggacctgg cgcatgtcgg cgacgaggcc
     1021 gatgcctgag tatctgggat ccgtttcgtt catcgcgtca cccctttgat gccaagcttt
     1081 cgatgtcgaa gtactggggc aggtccccga cgaggggcct gaggtagtcg aggaaagccg
     1141 ggctcacgtc gggcctgccg ggggcgagga aggcctcggg catgggcttg gcgcgcaccg
     1201 ccacctcgga cagtggggct gtcccgtagg aggcgcggta ggcggggccg gcctccctct
     1261 ggatggtcac catgagcccg ctcgccccgc ccagggccag ctcggcggcc ctgagcccgc
     1321 aggccatggc ctcctcgagg tcgaggccgc tcaccctgtc gtcggcgcac atgggcaggc
     1381 tctcggtgac ctggaactcg ccgcggaggc ccgtagcctc ccgcaggagg ccgtggatgg
     1441 ccagggccgc gctgccgccg cccatcgcgc cgaactcgac gttggagaag cggtccctcg
     1501 agctggtggc cgagacgggg ctcccgtcct cccagagcag tccctcgccg cagacgatcg
     1561 agcaccagcc ccattgggag atgcacgccc gcacctcgga cagcagggcc tcgcgcgtga
     1621 tggggcgctc gggcaggtag atgaggtggg gggcctcgcc cggccgcgag cgcgcgaggg
     1681 ccgaggcggc cgcgagccag cccgagtcgc ggcccacggt ctggtggacg acgaaggagt
     1741 ccaccctcct catgtccttg gcgagccgtc cggcctgcct gaccgagagg gccacgtagc
     1801 gggccgccga ggggtagccc ggcgtgtggt cggtgccgaa gaggtcgttg tccacggtct
     1861 tgggcacgcc gatcccgcgc agctcccagc catgggcccg ggaccaggcc tctacgcggt
     1921 ggatggtgtc catcgtgtcg ttcccgccga tgaggaagaa gtacctgatg tcgtgccgct
     1981 tcaaggcctc gaggatgcgc gggaagtcgg cctcggcgag cttgtgtctg caggagccca
     2041 gggcagagga gggtgtcctc gcgaggaggt cgagggcctc gcctccgagg gcgcccagat
     2101 cgacgaggag gtcctccatg aagccctcga tcccgaagcg catgccgaag accctgccga
     2161 tgcgggggtc ggccctgcag cccctgacca cgcccgcaag gctggcgttg atgacggccg
     2221 tgggccctcc cgactgtccg acgatcgcgt tgcccttcat ccttcgctcc tgtccctctg
     2281 tcgtgcgggg acaggatacg tctggccgag ttggcctgtc tagcccgagg ggctcggcgc
     2341 ccctgacggg gcgcggccgc gcgcaagggg ccgccgcgcc caaggggaca gccgcgggcc
     2401 gagggccgcc gcgggcgcct cctggagccc gccctagccc aggcccagac gctcccgctc
     2461 gacgaaggac aggagcctcc ctatctcggc cgcgcaacgc gccggagccg ggccgccgac
     2521 gaggtcgcgg cgggccacgc aggcctcgag cgagaggtag ggcgcgagct ccccgggctg
     2581 gaccccgctc caggccgtgt ggagggcggc gaggccaggc tcgccgaggg aggcgatgag
     2641 gccggccacc gaggggtttc ggccaaagcc cccgccaggc cccgcggccc gcgcccaggc
     2701 ctcgacgagg gcccggccgg cggcgtaggc ggtgcggaag ggcacggcct tgcccacgag
     2761 gaactcggcg gcgtcggccg cccagaggca gcccgcctcg gcgg
//
LOCUS       DASSQV010000341         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_206401, whole genome shotgun sequence.
ACCESSION   DASSQV010000341 DASSQV010000000
VERSION     DASSQV010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206401"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFL04_05955"
     CDS             complement(<1..234)
                     /locus_tag="VFL04_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7839283.1"
                     /translation="MTIGAIEAGGTKFVCGLLQADSADRAKPQLIARASIPTTTPAET
                     LEAALRFFAEPGSGRPRPDCLGIGCFGPVDLKAS"
     gene            complement(239..835)
                     /locus_tag="VFL04_05960"
     CDS             complement(239..835)
                     /locus_tag="VFL04_05960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7839284.1"
                     /translation="MIEIREYEQLAKDTGAFKDIELDMLKETLQAWKAKPGEPFTLLD
                     LRDGRILAGFAVLARAPDTDFTYDVRAICIERAYIGTGVGKRLIEMVEEEAMRVSNSA
                     IVRFETSRRKEDAIGRGVFTDAGYLSIGHIADFYEAGDDYFIYAKHLHRAIKAQEPGA
                     AETGPAAASAGGAGAGAASAGDAAEAKPRADAARRGGE"
     gene            complement(980..2170)
                     /locus_tag="VFL04_05965"
     CDS             complement(980..2170)
                     /locus_tag="VFL04_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET7839285.1"
                     /translation="MKRLLALFALVLVLGSAAFAQNKAALQIVCNQSGAQVFINGRLM
                     GTTSPNIALLLPVGQYAVKVSKSGFLAYDTSVNLGAGGAVINVNLVQPGAPQAQVPQA
                     QRMNVLPPPTVFNYSITVQCNVQGAQVIINGNPAGTTPFSSQVPGGSYSVLVRAPGYL
                     DFNQNVVVNGAMTVLANLVPISFSLSVNANVAGADVIINGNPAGKTPFSAQVPGGSYT
                     VTVRAAGYIDYNQNVVVSGAAQVNASLQPLSYQLNVVATNVNGAQVFLNGNPMGQTPF
                     VSMLPPGSYGLIVRAPGFLDYSAQVLLSAAQTVSATLVPAMATWQLNFPDAFINRELK
                     GAHLTLVLIFIDGVLQKGSGGQIPAGKHVVRLVTGGMATETQVELQAGRTYTFEPSLG
                     LSVR"
     gene            2362..>2912
                     /locus_tag="VFL04_05970"
     CDS             2362..>2912
                     /locus_tag="VFL04_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839286.1"
                     /translation="MKKKLIIVLLLVLVLGSSTYAQLVLGVSGALRADSTMSASSIAS
                     QYKSGQNIFYGPFVEIIFGKLGLGVSGNFSFFEDLGTKFMDYDVDGYVSYHLFGGRAF
                     LDPFGELGLGLMAYNYANSSDNPSTNDSPISGNYYWFGGFGLGLNLGPIGIFGKMDYN
                     FRINGHLQGKDDFGNSYDIPYYG"
BASE COUNT          467 a          892 c         1038 g          515 t
ORIGIN      
        1 cgaggccttg aggtcgacgg ggccaaaaca gccgatgccg aggcagtcgg gtctcgggcg
       61 gccggagccg ggctcggcga agaagcggag ggccgcctcc agggtctcgg cgggcgtggt
      121 ggtggggatg ctcgccctcg cgatgagctg gggcttggcc ctgtcggcgc tgtcggcctg
      181 gaggaggccg cagacgaact tggtcccgcc cgcctcgatg gcgccgatgg tcatctggct
      241 actccccgcc cctgcgggcc gcgtcggccc ggggctttgc ctcggctgcg tcgccggcgg
      301 aggcggcacc agccccggcg cctccggcgg aggccgccgc gggccccgtc tcggcagcgc
      361 ccggctcctg ggccttgatg gcgcggtgca ggtgcttggc gtagatgaag tagtcgtcgc
      421 ccgcctcgta gaagtcggcg atgtggccga tggagaggta tccggcgtcg gtgaagacgc
      481 cccggccaat cgcgtcctcc ttgcgcctcg aggtctcgaa cctcacgatg gcggagttcg
      541 agacgcgcat cgcctcctcc tcgaccatct cgatgaggcg cttgccgacc ccggtgccga
      601 tgtaggcgcg ctcgatgcag atcgcccgca cgtcgtaggt gaagtcggtg tcgggggccc
      661 tggccaggac ggcgaagccc gcgaggatcc ggccgtcgcg gaggtcgagg agggtgaagg
      721 gctcgcccgg cttggccttc caggcctgga gggtctcctt gagcatgtcc agttcgatgt
      781 ccttaaaggc gccggtgtcc ttggcgagct gctcatattc gcgtatttcg atcatgccac
      841 tttcttcctg attaatcctt atacccgcaa agtatagtcg atggacgggc gaaagtacac
      901 ccgaaagggg cctagggaac gggaaggccc gcgggggacg ccgcgggcct tcgtggatcg
      961 gggatctccg ggagagggcc tagcgcaccg agaggccgag ggagggctcg aaggtgtagg
     1021 tcctgcccgc ctggagctcg acctgggtct ccgtggccat gccgccggtc acgaggcgca
     1081 cgacgtgctt gcccgcgggg atctggccgc ccgaaccctt ctggaggacc ccgtcgatga
     1141 agatgagcac cagggtcagg tgggccccct tgagctcccg gttgatgaag gcatcgggga
     1201 agttgagctg ccaggtcgcc atggccggca cgagggtggc gctgaccgtc tgggccgccg
     1261 agaggaggac ctgggccgag tagtccagga agcccggggc gcggacgatg aggccatagc
     1321 tccccggggg cagcatgctc acgaaggggg tctggcccat ggggttgccg ttgaggaaga
     1381 cctgggcccc gttgacgttg gtggccacga cgttgagctg gtagctcagg ggctggaggc
     1441 tcgcgttgac ctgggccgcc cccgagacca ccacgttctg gttgtagtcg atgtagccgg
     1501 ccgcccgcac ggtgaccgtg taggagcccc cggggacctg ggcgctgaag ggggtcttgc
     1561 cggcggggtt gccgttgatg atgacgtcgg cgccggcgac gttggcgttc accgagagcg
     1621 agaagctgat cgggacgagg ttggccagga cggtcatcgc cccgttcacg acgacgttct
     1681 ggttgaagtc gagatagccg ggggcgcgca cgaggaccga gtaggagcct ccggggacct
     1741 gggagctgaa gggggtggtc cccgcggggt tgccgttgat gatcacctgg gcgccctgga
     1801 cgttgcactg gacggtgatg ctgtagttga agacggtggg cgggggtagg acgttcatcc
     1861 tctgggcctg gggcacctgg gcctgggggg cgcccggctg gaccaggttc acgttgatga
     1921 cggcccctcc cgcgccgagg ttcacgctcg tgtcgtaggc caggaagccg gacttggaga
     1981 ccttgacggc gtactggccc accggcagga ggagggcgat gttgggcgag gtcgtcccca
     2041 tgagccggcc gttgatgaac acctgggcgc cgctctggtt gcagacgatc tggagggcgg
     2101 ccttgttctg ggcgaaggcc gcggagccga gcacgagcac gagggcaaaa agggccaaca
     2161 gacgcttcat cttctacctc cgggagtacg ggagtgcagg tgttcgggta atccagccgc
     2221 tctcgacgtg cctcaactct attacattcg gccaggtctt tccactcccg ccggttcccg
     2281 ggcccgcagc cttgcgattt ctttcgggag cgctatagta atgaatgaga tttgagttgt
     2341 gagataccag gaggcaaagc cgtgaagaag aaactgataa tcgtcctctt gctcgtgctg
     2401 gtgctcggct ccagcaccta cgcccagctc gtgctcggcg tctcgggcgc cctcagggcc
     2461 gacagcacca tgtcggcgag ctccatcgcc agccagtaca agagcggcca gaacatcttc
     2521 tacgggccct tcgtggagat catcttcggg aagctcggcc tcggcgtctc gggcaacttc
     2581 tccttcttcg aggacctcgg caccaagttc atggactacg acgtcgacgg ctacgtttcc
     2641 taccacctct tcgggggcag ggccttcctc gaccccttcg gcgagctggg tctcggactc
     2701 atggcctaca attacgcgaa ctcgagcgac aacccgagca ccaacgacag cccgatctcg
     2761 ggcaactact actggttcgg cggcttcggc ctcggcctga acctcgggcc catcgggatc
     2821 ttcggcaaga tggactacaa cttcaggatc aacggccacc tccagggcaa ggatgacttc
     2881 ggcaatagct acgacatccc ctactacggg at
//
LOCUS       DASSQV010000342         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_207195, whole genome shotgun sequence.
ACCESSION   DASSQV010000342 DASSQV010000000
VERSION     DASSQV010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207195"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..581
                     /locus_tag="VFL04_05975"
     CDS             90..581
                     /locus_tag="VFL04_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HET7839287.1"
                     /translation="MPTDIESLIATIEAQEAELQFASFSNDEALELGLLLVRMARERG
                     AAVAIDISRGSQRLFHYAFAGTSADNDAWIERKKAVVARFGRSSFLIGRRLERDGLGI
                     AEKFFVDPREYSPHGGAVPIILRGTGPVGVVAVSGLPQAEDHELVVAALRAYLQAATP
                     LRG"
     gene            606..1307
                     /locus_tag="VFL04_05980"
     CDS             606..1307
                     /locus_tag="VFL04_05980"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7839288.1"
                     /translation="METQAADGLDLRSLSDLVYEDLKRRLNGGSLRPGQFIDLSALGR
                     ELGMSRTPLRDALIRLELEGFVRVYPRRGVMVRSLDLADIRDIYQILGALEASAALKS
                     EGRFRTEDADRMDCLVATMGSTLEADDFPAFYAANLAFHDVYLDLSDNRDLVRTVRTL
                     KERLYDFPRRTAFVKEWELASASEHVEIAARFRAGDFAGAAAVIRDVHWSYEAQELFI
                     RKYYFAAGGKVGAER"
     gene            1304..2251
                     /locus_tag="VFL04_05985"
     CDS             1304..2251
                     /locus_tag="VFL04_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET7839289.1"
                     /translation="MNSGTVFDIRKYSIHDGPGIRTAVFLKGCPLRCSWCHNPEGLEA
                     EPALLRRADRCIHCASCREACPRGLDPSGDTTGLAFIRGSAADRNCIHCGLCATACPS
                     EALQLVGRIMGVDEVMRAIREDLPFYEESGGGVTFSGGEPLLQSRFLAELLDACRAEG
                     IRTAVDTSGFADFEIFMDIARRADLILFDLKLMDAQRHLDATGAPLEPILVNLRELAR
                     IARGPRPPRIASVALRLPVVPGINDSDADLEAMAGFVAGLETPWPVHLLPYHTSALAK
                     YRMWGMQYRLGQCPAPSALRMGEVLGLFTRAGIPAKIGG"
     gene            2258..>2598
                     /locus_tag="VFL04_05990"
     CDS             2258..>2598
                     /locus_tag="VFL04_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HET7839290.1"
                     /translation="MNERIRALREESFAAHPSFSAERAVLTTEFYQEWSGKVSVPVLR
                     AMNFRNLCARKAIYIGELELIVGERGPRPKAVSSFPELTCHSEEDLRILDTRPMTSYS
                     VSEEDLRLYRER"
BASE COUNT          413 a          960 c          827 g          398 t
ORIGIN      
        1 gagccactat agcggcggaa ggggggggag ggccatgccc gcttcaatga tcctggcccc
       61 cgcgctatca tcgccccagg aggacagcca tgccgaccga catcgaatcg ctcatcgcga
      121 ccatagaggc ccaggaggcc gagctccagt tcgcctcctt ctccaacgac gaggccctcg
      181 agctcggcct cctcctcgtg aggatggccc gcgagcgcgg ggccgcggtc gctatcgaca
      241 tatcgagggg gagccagagg ctcttccact acgccttcgc cggtacctcg gcggacaacg
      301 acgcctggat agaaaggaag aaggccgtgg tcgcccgctt tggccgcagc tccttcctca
      361 tcggcaggag gctggagcgc gacggcctcg gcatcgccga gaagtttttc gtcgacccga
      421 gggaatactc cccccacggc ggggccgtcc cgatcatcct caggggcacg ggacccgtgg
      481 gcgtggtggc ggtctcgggc ctgccccagg ccgaggacca cgagctcgtg gtggccgccc
      541 tgagggcgta cctccaggca gcaacaccct tgcgcggctg atatatcagg gctatagtgg
      601 gcttgatgga gacccaagcc gccgacggac tcgacctccg ctccctctcg gacctcgtct
      661 atgaggacct gaagcgccgc ctcaacgggg gcagcctgag gccgggccag ttcatcgacc
      721 tctcggccct gggcagggag ctcggcatga gccgcacccc cctccgcgac gccctcatac
      781 gcctcgagct cgagggcttc gtccgcgtct atccgcgccg cggggtcatg gtccgcagcc
      841 tcgacctggc cgacatcagg gacatctacc agatcctcgg cgccctcgag gcctcggcgg
      901 ccctgaagtc cgagggccgg ttcaggaccg aggacgccga ccgcatggac tgcctcgtgg
      961 cgaccatggg ctcgaccctc gaggccgacg acttcccggc cttctacgcc gccaacctcg
     1021 cattccacga cgtctacctc gacctctccg acaaccgcga cctggtcagg acggtcagga
     1081 ccctcaagga gaggctctac gacttcccga ggcggacggc cttcgtgaag gaatgggagc
     1141 tcgcctcggc ctcggagcac gtggagatag ccgcgaggtt cagggccggg gacttcgccg
     1201 gggcggctgc cgtcatccgc gacgtccact ggtcctacga ggcccaggag ctcttcatcc
     1261 gcaagtacta cttcgccgcc ggcggcaagg tgggagccga gcgatgaact cgggcaccgt
     1321 cttcgacatc cgcaaatact cgatccacga cgggccgggg atcaggacgg ccgtcttcct
     1381 caagggctgc cccctccgct gctcctggtg ccacaacccc gagggcctcg aggccgagcc
     1441 cgccctcctc agacgggccg accgctgcat ccactgcgcc tcctgccgcg aggcctgccc
     1501 ccgcggcctc gacccgagcg gggacaccac gggtctggcc ttcatccgcg gctcggccgc
     1561 cgaccgcaac tgcatccact gcgggctctg cgcgacagcc tgcccctccg aggccctcca
     1621 gctcgtcggc cgcatcatgg gcgtcgacga ggtcatgcgg gccatcaggg aggacctgcc
     1681 cttctatgag gagtcggggg gaggggtgac cttctcgggg ggcgagccgc ttctgcagag
     1741 ccgcttcctc gccgagctcc tcgacgcctg cagggccgag gggatcagga cggccgtcga
     1801 cacctcgggc ttcgccgact tcgagatctt catggacata gcgcggcggg ccgacctcat
     1861 cctcttcgac ctcaagctca tggacgccca gaggcacctc gacgcgacgg gggcccccct
     1921 cgagcccatc ctcgtcaacc tgcgcgagct cgcccggatc gcccgcggac ccaggcctcc
     1981 ccggatcgcc tcggtggccc tgcgcctccc cgtcgtgccg ggcatcaacg actcggacgc
     2041 cgacctcgag gccatggccg gtttcgtggc cggcctcgag acgccctggc ccgtccacct
     2101 ccttccctat cacacctcgg ccctcgccaa ataccggatg tggggcatgc aataccgcct
     2161 tggccagtgt cccgctccct cggcccttcg gatgggcgag gtcctcgggc tgttcaccag
     2221 ggcggggatc cccgcgaaga tcggaggcta ggaccacatg aacgagagga tacgagcgct
     2281 ccgcgaggag agcttcgccg cccacccgag tttctcggcc gagcgggcag tgctgacgac
     2341 cgagttctac caggagtggt cgggcaaggt ctcggtgccc gtgctccgcg ccatgaactt
     2401 ccgcaacctc tgcgcgagga aggccatcta catcggcgag ctcgagctca tcgtgggcga
     2461 gcggggaccc aggcccaagg ccgtctcctc cttccccgag ctcacctgcc actccgagga
     2521 ggacctcagg atcctcgaca cgaggcccat gacgagctac tccgtctccg aggaggacct
     2581 gcggctctac cgcgagcg
//
LOCUS       DASSQV010000343         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2071934, whole genome shotgun sequence.
ACCESSION   DASSQV010000343 DASSQV010000000
VERSION     DASSQV010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071934"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFL04_05995"
     CDS             <1..388
                     /locus_tag="VFL04_05995"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET7839291.1"
                     /translation="AVRLKLHAVPEVVSGKRVVMIDDSLVRGTTSRIIVNLLREAGAR
                     EVHLRLAAPELKWPCYYGIDIPDRDQLISNRLGPEEIAEELGADSARFLPVERLIAAV
                     GGGGYCSACFDGRHPYAPKSGEKGEA"
     gene            385..1347
                     /gene="purM"
                     /locus_tag="VFL04_06000"
     CDS             385..1347
                     /gene="purM"
                     /locus_tag="VFL04_06000"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET7839292.1"
                     /translation="MSGLDYQKAGVSIRKGDEFAEFIKAFPSKAVSKGLGGFAGGSPL
                     DLRGFREPLLLSTTDGVGTKLLVAKKLGRYDTIGIDLVAMSVNDLVVCGAKPLQFLDY
                     IACGRIHEGVLQEIIGGIIAGCEQAGCELTGGETAEMPDVYGEDDIDLAGFAVGIVEK
                     AKQLPRLERMEAGDLLLGLPSTGVHSNGYSLARKAIDPDDAGLWSELLVPTKIYVREL
                     MELMSTGRVLGAAHITGSGLAGNVERVIPRGLKASFAWNWPVPPIFAAIQAGGRIGDE
                     EMRSVFNMGIGMVLIAKKEEAAGLIEIGRAKSIPLLEIGTLVSG"
     gene            1340..1927
                     /gene="purN"
                     /locus_tag="VFL04_06005"
     CDS             1340..1927
                     /gene="purN"
                     /locus_tag="VFL04_06005"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET7839293.1"
                     /translation="MAKLAVLASGKGSNFEALVLALRASSRAHECVLLLADRKDALAL
                     ARAERLGIPARLVAYGGRPRREAEAELADAIRGSGADLVALAGFMRILGPDFVRLHRG
                     RMVNVHPSLLPKWPGRDAIRRAYEAGETVFGVSVHYVDEGMDTGPVISCSSFTPERGS
                     SLESIEARVHEIEHRLFPETLIGLLDRIERDGKAK"
     gene            1924..>2823
                     /gene="purD"
                     /locus_tag="VFL04_06010"
     CDS             1924..>2823
                     /gene="purD"
                     /locus_tag="VFL04_06010"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET7839294.1"
                     /translation="MNTLVVGSGAREHAIAWTLARDPDCSSGKGPRERIFVAPGNAGT
                     AEFATNLALDPADPLAVIGACRELGIGLVIIGPENPLALGLVDALAVAGIPAFGPPAT
                     AARLESSKSFARSFSERAGVPSAKTARFTSVPELRRFLDELGGRRVVLKKSGLAAGKG
                     VFESDDPDELLAFGRDVLQGDELLAEEFLTGTELSVFALCDGGDRLVLPACADHKKAK
                     AGDSGANTGGMGAVCPVPAANASLMARIEREIVDPSFRAMEREGLLDRGVLFFGIMNT
                     SDGPKLLEYNVRFGDPEAQSLLPL"
BASE COUNT          504 a          906 c          926 g          487 t
ORIGIN      
        1 ggccgtgagg ctgaagcttc atgccgtgcc ggaggttgtc tcgggcaagc gcgtcgtcat
       61 gatcgacgat tcgctggtca ggggcacgac gagccgtatt atcgtcaatc tgctccgcga
      121 agccggggcg cgggaagtcc atcttcgact cgccgctccc gagctcaaat ggccctgcta
      181 ttacggcatc gacattccgg accgggacca gctcatctca aatcgcctcg gaccggagga
      241 aatagcggag gagctgggcg cggattccgc gcgtttcctc cctgtggagc ggctcattgc
      301 cgcggtcgga ggcggaggct attgcagcgc ctgtttcgac ggcagacatc catacgcccc
      361 aaaaagcgga gaaaagggag aagcatgagc ggtctcgact atcagaaggc tggagtgtcc
      421 atacgcaagg gcgacgagtt cgccgagttc atcaaggcat tcccctcaaa ggcagtctca
      481 aagggcctgg gcggtttcgc tggaggcagc cccctcgacc tgcgcgggtt cagggagccc
      541 ctcctgctct cgaccacaga cggggtcggc acgaagctcc tcgtggccaa gaagcttggc
      601 cgctatgaca cgatcggcat cgacctagta gcgatgagcg tcaacgacct ggtcgtctgc
      661 ggggccaagc ccctccagtt cctcgactac atagcctgcg gaaggatcca cgagggagtc
      721 ctccaggaga tcatcggcgg catcatcgca ggctgcgagc aggcgggctg cgagctgaca
      781 ggtggcgaga ccgccgagat gcccgacgtc tatggcgagg acgacatcga cctagcgggt
      841 tttgcggtcg ggatagtcga gaaggcaaag cagctgccca ggctcgagcg catggaggcg
      901 ggagacctcc tcctcggcct ccccagcacg ggggtgcact ccaatggcta tagcctggcg
      961 cgcaaggcga tagatccgga tgacgccggt ctctggagcg aacttctcgt gcccacgaag
     1021 atctatgtga gggaacttat ggagctcatg agcacgggca gggtcctcgg cgcggctcac
     1081 atcacgggct cgggtctcgc aggcaacgtg gagcgagtga tcccccgcgg gctcaaggcg
     1141 agtttcgcct ggaactggcc cgtccccccc attttcgccg ccatccaggc cggaggacgg
     1201 atcggggacg aggagatgcg ctcggtgttc aacatgggca tcggcatggt cctcatagcg
     1261 aaaaaggagg aggctgccgg cctcatcgag atcggaaggg ccaagagcat ccccctgctc
     1321 gagatcggga ccctggtcag tggctaaact cgccgtcctc gcgagcggca agggcagcaa
     1381 cttcgaggcc cttgtcctgg ccctccgcgc ctcgagccgg gcgcacgaat gcgtccttct
     1441 cctggccgac aggaaggatg ccttggccct ggcgagggcc gaacgtctcg gcatacccgc
     1501 gcgcctcgtc gcctatgggg gccgaccgcg gcgggaggct gaggccgaac ttgcggatgc
     1561 gatccgcgga tcaggcgccg atctcgtggc cctcgccggc ttcatgcgca tccttggtcc
     1621 cgatttcgtg aggctccatc ggggcagaat ggtgaacgtc catccctccc tcctgcccaa
     1681 gtggccgggc agagacgcca tcaggagggc ctacgaagca ggcgagactg tgttcggcgt
     1741 cagcgtccat tacgtggatg agggcatgga cacgggcccc gtcatctcat gctcctcctt
     1801 cacccccgaa cgcggctcaa gcctcgagtc catcgaggcg cgggtgcatg agatcgagca
     1861 tcgcctcttt ccggagaccc tcattgggct cctcgaccgc atcgaaaggg atgggaaggc
     1921 aaaatgaaca ccctcgtcgt cggatcgggc gcacgggagc acgccatagc atggaccctc
     1981 gcgcgcgatc ccgactgcag ttcggggaaa gggccgaggg agcgcatttt cgtcgcgccc
     2041 gggaacgcgg gcacggccga gttcgcgacg aatctcgccc tcgatcccgc cgaccctctc
     2101 gccgtcatcg gggcctgccg ggaattgggc atcggcctcg tcatcattgg acccgagaac
     2161 cctctcgccc tgggcctggt cgacgccctt gcggtggcgg ggatcccggc ctttggcccg
     2221 cccgccacgg ccgcgaggct tgaatcgagc aaatccttcg cacgatcctt ttcggagcgg
     2281 gcaggcgtgc catcggcaaa gactgcgcgc ttcacgtctg tccccgaact gcgccgtttc
     2341 ctcgacgagc ttggtggtcg gcgcgtcgtc ctcaagaaga gcggtctggc cgccggcaag
     2401 ggggtcttcg aatccgacga tcccgatgag ctccttgcct tcgggcgcga tgtgctgcag
     2461 ggcgacgagc tccttgccga ggaattcctc accggaaccg agctctcagt attcgcgctc
     2521 tgcgatggcg gcgaccgcct tgtcctgccg gcttgtgccg accacaagaa ggcgaaagcc
     2581 ggagacagtg gggccaatac cgggggaatg ggcgccgtct gccccgtccc cgcggccaac
     2641 gcctcgctga tggcgaggat cgagcgcgag atcgtcgacc cgagcttcag ggccatggag
     2701 agagagggcc tgctcgaccg tggtgtcctt ttctttggca tcatgaacac ctcggatggt
     2761 ccgaagctcc tcgagtacaa tgtgcgtttc ggagaccccg aggcccagag cctccttccc
     2821 ctc
//
LOCUS       DASSQV010000344         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2072004, whole genome shotgun sequence.
ACCESSION   DASSQV010000344 DASSQV010000000
VERSION     DASSQV010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072004"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VFL04_06015"
     CDS             <1..943
                     /locus_tag="VFL04_06015"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET7839295.1"
                     /translation="IGLYLLHGRATGVYSCYYRLLLGTALSPGAGMLLMLGFFAAFAV
                     KRPAFPLHAWLPDAHPEAPTAGSVVLAGLLLKTGGYGLIRFVLPLFPEASRSFGPVAV
                     WIGVAGILYGGFMAFSQTDMKRLVAYTSVSHLGFVLLGIFAFNVQAMNGAVIQMLSHG
                     LSTGALFILVGSLQDRLHTRELAKMGGLWTAAPRFGAFGLVLTMALLGLPGFGNFIGE
                     FLVLLGAYHASVAASVAAAIGLVFAMVYSLRMFQLAFHGERSDQREVRDLGPIEIAAL
                     SLMILALFWLGLFPQASMDAASRALGAILGLVQGAAA"
     gene            940..2373
                     /locus_tag="VFL04_06020"
     CDS             940..2373
                     /locus_tag="VFL04_06020"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET7839296.1"
                     /translation="MNHDDLFSLLPLLVLAGSAVLVLFGLAFSKRRYETAFALSMAGL
                     IAAALSLGLAWPLAPRRFTALLVLDRFAYLSMALTIAATIVVALMSFAYFRGRREESG
                     EFYFLLQLACLGCAILGSSSSFASFFLGLELLSTPLFTLIGYRRDREAGVRSAFMYLI
                     LAGVSSAFLLFGMALVYLETGSLDISAAARAASAGQGFAVLAGLALMTVGIGFKLAVA
                     PFHVWTPDIYDGAPAPATAFIATASKGAMAVLLVRFFAPAGIASVGPFAWIFALIAAV
                     SMFTGNILALREGNLKRLLAYSSIAHLGYILVAFVASGSAAGAAVSFYLVAYFVSNLG
                     AFAAVSGLSTDASEADSIESYRGLAARHPWTAAALTASLLSLAGLPLTAGFMGKFVIF
                     AAGAGALLWVLIVILAVNSTISIFYYLRVVSALYRPAPEAGPGSARPGLPALAALALG
                     AMAVAIIVIGVLPDSLIAFVREVAATL"
     gene            2400..>2784
                     /locus_tag="VFL04_06025"
     CDS             2400..>2784
                     /locus_tag="VFL04_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7839297.1"
                     /translation="MRERLLGKTGFRVSEIGLGTWQLGGVWGADFDDGLADRTIGAAL
                     DSGVNFIDTADVYSDGRSETAVGRALRARPGAFRVATKCGRRISPHVSEGYRVEVLRG
                     FVEDSLRRLGTDCIDLIQLHCPPTEV"
BASE COUNT          322 a         1107 c          837 g          518 t
ORIGIN      
        1 catcggcctc tacctcctcc acggcagggc cacgggggtc tacagctgct actaccgcct
       61 cctcctcggc acggccctga gcccgggcgc cggcatgctc ctcatgctcg gcttcttcgc
      121 ggccttcgcg gtcaagcggc cggccttccc cctccacgcc tggctccccg acgcccatcc
      181 cgaggcgccc acggccggca gcgtggtcct cgcgggcctc cttctcaaga ccggtggcta
      241 tggcctcatc cgcttcgtcc tgccgctttt ccccgaggcc tcgcggagct tcggccccgt
      301 cgcggtctgg atcggcgtcg ccggcatcct ctacggcggc ttcatggcct tcagccagac
      361 cgacatgaag cgcctcgtgg cctacacctc ggtgagccac ctcggcttcg tcctactggg
      421 catcttcgcc ttcaacgtcc aggcgatgaa cggggcggtg atccagatgc tgagccacgg
      481 cctctccacg ggcgccctct tcatcctcgt gggctcgctc caggacaggc tccacacgcg
      541 ggagctggcg aaaatgggcg ggctctggac ggcggccccg aggttcgggg ccttcggcct
      601 cgtcctcacg atggccctcc tgggcctgcc gggcttcggc aacttcatcg gcgagttcct
      661 cgtcctcctc ggcgcctacc acgcgagcgt ggcagcctcg gtggccgctg cgatcggcct
      721 cgtcttcgcg atggtatact cgctgcggat gttccagctc gccttccacg gcgagcgcag
      781 cgaccagcgc gaggtcaggg acctcgggcc catcgagatc gcggccctca gcctcatgat
      841 cctggccctc ttctggctgg gcctattccc ccaggcatcg atggacgcgg cctcgcgcgc
      901 cctcggcgcc atcctgggcc tcgtgcaggg agccgccgca tgaaccatga cgacctgttc
      961 tccctcctcc ccctcctggt cctggcgggc agcgccgtcc tcgtcctctt cggcctcgcc
     1021 ttctccaagc gccgctacga gaccgccttc gccctctcca tggcaggcct catcgccgcc
     1081 gccttgagcc ttggcctcgc ctggcccctg gccccgcggc gcttcaccgc cctcctcgtg
     1141 ctggaccgct tcgcctatct ctcgatggcc ctcacgatcg ccgctaccat cgtcgtggcc
     1201 ctcatgtcct tcgcctactt ccgcggcagg agggaggagt cgggcgagtt ctacttcctc
     1261 ctccagctgg cctgcctcgg ctgcgccatc ctcggctcga gctcgagctt cgcctcattc
     1321 ttcctcgggc tcgagctcct cagcactccc ctgttcaccc tgataggcta ccgccgcgac
     1381 cgcgaggccg gggtgaggtc ggccttcatg tacctcatcc tcgccggcgt ctcctcggcc
     1441 ttcctcctct tcggcatggc cctggtctac ctcgagacgg ggagtctgga catctcggcg
     1501 gcggccaggg ccgcctcggc cgggcagggc ttcgccgtcc tcgctggctt ggccctcatg
     1561 accgtgggca tcggcttcaa gctggccgtc gcccccttcc acgtctggac ccccgacatc
     1621 tacgacggag ccccggcccc agccacggcc ttcatcgcga ccgcctccaa gggggcgatg
     1681 gccgtcctcc tcgtgcgttt cttcgccccg gccgggatcg cctcggtggg gcccttcgcc
     1741 tggatcttcg ccctgatcgc cgccgtctcg atgttcacgg gcaacatcct cgcgctcagg
     1801 gagggcaacc tcaagcgcct cctcgcctac agctcgatcg cccatctcgg ctacatcctc
     1861 gtcgccttcg tggcgagcgg gagcgcggcc ggggccgccg tgtccttcta cctcgtcgcc
     1921 tacttcgtct ccaacctcgg ggccttcgcg gcggtgtccg ggctctcgac cgacgcctcc
     1981 gaggccgact cgatagagtc ctacaggggc ctggccgccc gacatccctg gacggcggcg
     2041 gccctcaccg cctccctcct gtccctcgcc ggcctccccc tcaccgcggg cttcatgggc
     2101 aagttcgtga tcttcgcggc gggcgcggga gccctccttt gggtcctcat cgtgatcctc
     2161 gccgtgaaca gcaccatctc gatcttctac tacctcaggg tcgtgtcggc cctctaccgc
     2221 ccagcgcccg aggccggacc cggctcggca cggcctggcc tgcctgccct cgctgccctg
     2281 gccctgggcg ccatggcggt cgccatcatc gtcatcggcg tcctgccgga ttcgctcatc
     2341 gccttcgtca gggaggtggc ggcgaccctc tagcgggccc gaaggaggca agacagacca
     2401 tgcgcgaacg tctgctcggc aagacaggct tcagggtttc ggagatcggg ctcggcacct
     2461 ggcagctcgg cggagtctgg ggcgccgact tcgacgacgg cctcgccgac cgcaccatcg
     2521 gggcggccct cgactccggc gtgaacttca tcgacacggc cgacgtctat tccgacggcc
     2581 ggagcgagac ggcggtcggc agggccctcc gcgcgaggcc gggcgccttc agggtcgcca
     2641 ccaagtgcgg ccgccgcatc agcccccacg tgagcgaggg ctatcgggtc gaggtcctcc
     2701 gcggcttcgt cgaggacagc ctcaggcggc tcggcaccga ctgcatcgac ctcatccagc
     2761 tccactgccc gccgaccgag gtct
//
LOCUS       DASSQV010000345         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_81852, whole genome shotgun sequence.
ACCESSION   DASSQV010000345 DASSQV010000000
VERSION     DASSQV010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81852"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFL04_06030"
     CDS             complement(<1..195)
                     /locus_tag="VFL04_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET7839298.1"
                     /translation="MKDTLPEPAGEAATELGTDLPDRRVRYENLPVVVIAGRPNVGKS
                     TLFNRLLHKRRAITDPTPGVT"
     gene            complement(203..1927)
                     /gene="ptsP"
                     /locus_tag="VFL04_06035"
     CDS             complement(203..1927)
                     /gene="ptsP"
                     /locus_tag="VFL04_06035"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET7839299.1"
                     /translation="MIEYRGIPASPGVAVAPAFVIIDEGEMPVPAYSLEAADVPSEWA
                     RFLVAVEKARAEVTALRDRARNEAGDEQAAIFEAHLLMMDDPDLIERIERSLGATLRN
                     VERVIAEIERELVEKLSKASDPALRERATDIRDVSRRILGHLMLRERFTLADLEADIV
                     LVAKDLLPSDMISMNRARVKAIAMEAGGKTSHVAILARAFEIPAVLGIGASIGSLRSG
                     SLLAVDGDLGTVISEPDAAALARSAAAQEAAALRDRELEGLRDLPAVTTDGERVLVKA
                     NIEMPEEAMAALAHGAEGVGLFRSEFLFLGPHHVPGEEEQYRAYSRVISAMNGRPVTI
                     RTLDIGGDKVLPELGAQDEKNPLLGWRAIRFCLSNVELFKSQLRAILRAANHGDVRIM
                     FPMISTIDELERALAILAEARAECRARGHEVPDRIPTGIMVEIPSAAVASDILATKAD
                     FFSIGTNDLIQYTMAVDRGNGRVAYLHEPYHPAVLRLVKMTIESGRRARIPVAMCGEM
                     AADPLASVLLLGLGLDEFSMSSASVPAVKRALRSADMKEARAVAEAVLSLSSSAEVAA
                     YLSSRVRS"
     gene            complement(2090..3028)
                     /locus_tag="VFL04_06040"
     CDS             complement(2090..3028)
                     /locus_tag="VFL04_06040"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7839300.1"
                     /translation="MDEARRTATLSRIGQVLSRRPFRLALGALACALVLGGSAFRTGE
                     PNLTSLGSMDIARPNLAEVTSADPSADRGLYYTAYQVAKGDTASEIADRFDVSLDTVI
                     SFNDIQAARALKPGQILKIPSMAGILYTAKEGDSPASLALSNKISADRIIEANGLMSE
                     TLEPGRTYFLPDAHLASFRLREIAGDLFRWPVRGWITSWYGYRSDPFSGTRGFHNGLD
                     IGVDSGTPVLAAMEGVVAETGYSAMSGNYILIAHHSGWSSFYGHLYSIGVKAGQRVAA
                     GARIAFSGNTGYTTGPHLHFSVFKNGRSVNPSNVLH"
     gene            complement(3135..>3753)
                     /locus_tag="VFL04_06045"
     CDS             complement(3135..>3753)
                     /locus_tag="VFL04_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET7839301.1"
                     /translation="SPPKRGASPARRPDLSRYMGATVTAFEAIAAGASGEARFAENEN
                     DIVTQLSAILDRAAGQTTELVICMDTTESMLNDIEAMRKKAPAMLRSRLKDFPSLRLG
                     IVLYRDYFEEYLTKRLDFTSELGVFEEELKAIRVTGGRDIPEAVYEALNSALVEFPWS
                     AQRRLVVLIGDAPPHPIPRGSVDAATVQEAASRLGVEMDVIILPE"
BASE COUNT          558 a         1166 c         1403 g          626 t
ORIGIN      
        1 ggtcacgccg ggggtcgggt cggtgatggc ccgccttttg tggaggagac ggttgaagag
       61 ggtcgacttg ccgacgttgg gcctgcccgc gatgacaacg acgggcaggt tctcgtagcg
      121 gacgcggcgg tccgggagat cggtgccgag ctcggtggcg gcttcgccgg caggctcggg
      181 gagggtgtct ttcatggcgt gttcagctcc ggacgcgcga ggagagatag gcggccactt
      241 cggccgacga tgagagggag agcacagcct cggctaccgc ccgcgcttcc ttcatgtccg
      301 cggagcgcag ggcgcgcttg acggcgggca ccgaggcgct cgacatggag aactcgtcga
      361 gcccgaggcc gaggaggagg acagaggcga gggggtcggc ggccatctcg ccgcacatgg
      421 cgacggggat cctggccctc ctcccgctct cgatggtcat cttcacgagc ctgaggacgg
      481 cgggatggta gggctcgtgg aggtaggcga cccggccgtt gccgcggtcg acggccatcg
      541 tgtactggat gaggtcgttg gtgccgatcg agaagaagtc ggccttcgtc gcgaggatgt
      601 cgctggcgac cgcggccgag gggatctcca ccatgatgcc cgtggggatc cggtcgggca
      661 cctcgtggcc gcgggcgcgg cactcggccc tggcctcggc gaggatggcg agggcccgct
      721 cgagctcgtc gatcgtcgag atcatgggga acatgatgcg gacgtcgccg tgattggcgg
      781 ccctcaggat ggcgcgcagc tggctcttga aaagctcgac gttcgagagg cagaagcgga
      841 tggcgcgcca gccgaggagg gggttcttct cgtcctgggc cccgagctcg gggaggacct
      901 tgtcgccccc gatgtccagg gtcctgatgg tgacgggccg gccgttcatg gccgagatca
      961 cgcgggaata ggcccggtac tgctcctcct ccccggggac gtggtggggg ccgaggaaga
     1021 ggaactcgga gcggaaaagg ccgacgccct cggccccgtg ggccagggcg gccatggctt
     1081 cctcgggcat ctcgatgttg gccttgacga ggacccgctc cccgtcggtg gtgacggcgg
     1141 gcaggtcgcg caggccctcg agctcgcggt cgcggagggc cgcggcctcc tgggcggcgg
     1201 cgctcctggc gagggcggcg gcgtcgggct cggagatcac cgtgcccagg tcgccgtcca
     1261 cggcgaggag gctgccgctc cgcagcgagc cgatggaggc cccgatgccg aggacggccg
     1321 gtatctcgaa ggccctggcg aggatggcga cgtggctcgt ctttcccccg gcctccatgg
     1381 cgatcgcctt gacccgtgcg cggttcatcg agatcatgtc cgagggcagg aggtccttcg
     1441 ccaccaggac gatgtcggcc tcgaggtcgg cgagggtgaa gcgctcgcgg agcatgaggt
     1501 ggccgaggat ccggcgggag acgtccctga tgtcggtggc gcgctcgcgc agggcggggt
     1561 cgctggcctt ggacagcttc tccaccagct cccgctcgat ctccgcgatg acgcgctcca
     1621 cgttgcgcaa ggtggcgccg aggctcctct cgatcctctc gatgaggtcg gggtcgtcca
     1681 tcatgaggag gtgggcctcg aagatcgcgg cctgctcgtc ccccgcctcg ttgcgcgccc
     1741 gatccctcag ggccgtgacc tcggccctgg ccttctcgac cgcgacgagg aaacgcgccc
     1801 actccgaggg cacgtcggcg gcctcgagcg agtaggcggg cacgggcatc tccccctcgt
     1861 cgatgatgac gaaggcaggg gcgacggcga cgccggggga cgcggggatt ccgcggtatt
     1921 caatcatggg acttgaatgt atcagaaata cgatccagcg tcaaaaggcg ggtgcgcgcg
     1981 gctccagccc cggccaacaa gcttcgaggc aggggccgcc aacaggcttc gaggaagggg
     2041 ccgctggctg tgagaggctg gccgggggcc atgtccggcc ctggcggccc tagtgcagga
     2101 cgttcgaggg gttgaccgac cgaccgttct tgaagaccga gaaatggagg tggggacccg
     2161 tcgtgtagcc ggtgttgccc gagaaggcga tccgcgcccc ggcggcgacc ctctgccccg
     2221 ccttgacccc gatcgagtac aggtggccat agaagctcga ccagcccgag tggtgggcga
     2281 tgaggatgta gttgcccgac atggccgagt agccggtctc ggccacgacg ccctccatgg
     2341 cggcgaggac gggggtgccc gagtcgaccc cgatgtcgag gccgttgtgg aagcccctgg
     2401 tgccgctgaa gggatcgctg cggtagccgt accagctcgt gatccagccc ctgaccggcc
     2461 agcggaagag gtcgccggcg atctccctga gccggaacga ggcgaggtgg gcgtcgggca
     2521 ggaaataggt cctccccggc tcgagggtct cgctcatgag gccgttggcc tcgatgatgc
     2581 ggtcggccga gatcttgttc gagagggcga gggaggcggg gctgtccccc tccttcgccg
     2641 tgtacaggat gcccgccatg ctggggatct tgaggatctg gcccggcttg agggcgcgcg
     2701 cggcctggat gtcgttgaag ctgatgacgg tgtcgaggct gacatcgaag cggtcggcta
     2761 tctcgctggc cgtgtcgccc ttggcgacct ggtaggccgt gtagtagagg ccccggtcgg
     2821 cgctcgggtc ggcgctcgtg acctcggcga ggttggggcg cgcgatgtcc atggagccga
     2881 ggctggtgag gttcggctct ccggtcctga aggcagagcc gccgaggacc agggcgcagg
     2941 cgagcgcgcc cagggcgaga cggaagggcc gtcgcgacag gacctgtccg atccggctca
     3001 atgttgctgt ccttcgtgcc tcgtccatgc tcgctccact tcttgaaacg gttttccgcg
     3061 tggtctgggc cggtcgtttc gaacggcctt ccctccggaa tctacccgaa aatccatcgg
     3121 ggcgcaagcc tcgatcattc aggcaatata attacgtcca tctccacccc cagcctcgac
     3181 gccgcctcct ggacggtggc cgcatcgacc gagccccttg gtatggggtg gggaggggcg
     3241 tcgccgatca gcacgacgag acgccgctgg gccgaccagg ggaactcgac aagggcggag
     3301 ttcagggcct cgtagacggc ctcggggatg tcgcggccgc ctgtgacgcg tatcgccttg
     3361 agttcctcct cgaacacgcc gagctcggag gtgaaatcca ggcgtttggt caggtactcc
     3421 tcgaaatagt cccgatagag gacgatcccg agcctgagcg aggggaaatc cttgagcctc
     3481 gagcggagca tcgccggggc cttcttcctc atggcctcga tgtcgttgag catcgactcc
     3541 gtggtgtcca tgcagatcac aagctctgtc gtctggccgg cggctcggtc gaggatggcc
     3601 gaaagctggg tgacgatatc gttctcgttc tcggcgaagc gggcctcgcc cgaggctccg
     3661 gcggctatcg cctcgaaggc cgtcaccgtg gctcccatgt agcgggagag gtcgggcctc
     3721 ctggcgggac tcgctccgcg cttgggcggg ctc
//
LOCUS       DASSQV010000346         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_456324, whole genome shotgun sequence.
ACCESSION   DASSQV010000346 DASSQV010000000
VERSION     DASSQV010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456324"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFL04_06050"
     CDS             <1..315
                     /locus_tag="VFL04_06050"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7839302.1"
                     /translation="AVVIPAWMKRVYRHDPVRFVQFAVRVWGVDQSFRDAETTALEGI
                     ARLEAFWASIGLELRLPGLGIGPERIEEMASKCTSGDRAKVGHFVPLGATEVAEVLRL
                     AL"
     gene            552..998
                     /gene="fucU"
                     /locus_tag="VFL04_06055"
     CDS             552..998
                     /gene="fucU"
                     /locus_tag="VFL04_06055"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HET7839303.1"
                     /translation="MLIGIAPCISPELLALLHRMGHGDELVLADAFFPGDAMNPRVLR
                     ADGIRIPELLDGILALINPDSYVDAPFVMMAPVPGDELDPKVEAAYRAEIDRHWPDSP
                     PIARLERFAFYERAKKAFAVVMTGETVKYGNIIVKKGVIPLGPRTR"
     gene            complement(1004..1447)
                     /locus_tag="VFL04_06060"
     CDS             complement(1004..1447)
                     /locus_tag="VFL04_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839304.1"
                     /translation="MDAEAERFWSEFERETGEKVVARAVGSWYEDGSDERGVWGLVVL
                     TDASFRFKYLPSENWFASLFRAARREPARAVADITVPRESLVAMKEPHRTLASRLFGS
                     AFPRFTLSWREGELLRSEAFAVDPSTELLPRLREHFSPHAGKERG"
     gene            1513..2562
                     /locus_tag="VFL04_06065"
     CDS             1513..2562
                     /locus_tag="VFL04_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7839305.1"
                     /translation="MTGGEGQGPGVTIRDVARAAGVSTATVSRVLNADARVSPETALR
                     VRASVDELGYKMNQVARSLKTRATRTIGVVAPRLASDFFMLLAESMDRELSAHGYGLF
                     VCSSRESVREEEERLHLMAERLVDAVVMIPATDRGAHYRPFMGRGTPFLLVDRLVSDL
                     SADAVLVDNAGGSRDATRALISEGHRRIGFLGGSLDVSTARERYEGYRAAMAEAGLPV
                     ERDFVRFGSLHVESGYRSMAELLARPGAPEAYFIVNADTHVGATNYLMTEGRAFSRRI
                     VFASFDEMPYSPLLQFCRWSVSQPIDELGAQAARMALARIGGSRPAEAEVLRLQTRLI
                     RHEGRAHSTETDEIE"
     gene            2580..>3429
                     /gene="fucI"
                     /locus_tag="VFL04_06070"
     CDS             2580..>3429
                     /gene="fucI"
                     /locus_tag="VFL04_06070"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969638.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET7839306.1"
                     /translation="MANVSGMKVLPPAMRLKGGLPKIGIRPTIDGRLGGVRESLELPT
                     MGMARAAARLISETLRHPSGHPVECVVADTTIGGVAEAAACAEKFAREGVVATLTVTP
                     CWCYGSETMDMDPLTLKAVWGFNGTDRPGAVYLAAVLAAHNQKGLPAFGIYGRDVMDL
                     ADTDRIPADVAEKILRFARAALAAGWMRGKSYLSVGSVSMGIAGSIVDPDFFQAYLGM
                     RSEYVDMSELVRRFEEKIYSEAEFRRALDWVKENCKEGPDVNDPKAQHSRERKDKAWA
                     TSVKMAM"
BASE COUNT          536 a         1225 c         1183 g          485 t
ORIGIN      
        1 gccgtcgtca tccccgcctg gatgaaacgc gtctaccgcc acgacccggt ccgcttcgtc
       61 cagttcgccg tcagggtctg gggggtcgac cagagcttcc gcgacgccga gacgacggcc
      121 ctcgagggca tcgcgaggct cgaggccttc tgggcctcca tcggcctcga gctcaggctg
      181 cccggcctcg gcatcgggcc cgagcggatc gaggagatgg cctcgaaatg cacctcgggg
      241 gacagggcca aggtcgggca tttcgtcccc ctcggggcca cggaagtggc cgaggtcctc
      301 aggctggccc tctagggtga cgaagattgc ggctgccccg ccccggcctt catgggccgg
      361 ggcgggcgag gcccgggccg ggcggagccc tggaccgggc ggctgcgccc ccaaaggccg
      421 cgccctcggc cgctcccctc tccgtcatgt aatcgtttac agatttgatc ttgacaggac
      481 ttgcattccg gtaaacgatt gccccgcggg atcgggctgc gccaagggcc gccgtcccga
      541 ggaggtcaga catgctcatc ggcatcgcgc cctgcatatc gcccgagctg ctcgccctgc
      601 tccacaggat gggccacggc gacgagctcg tcctcgccga cgccttcttc ccgggcgacg
      661 cgatgaaccc gagggtgctc agggccgacg ggatccggat ccccgagctc ctcgacggga
      721 tcctcgccct catcaacccg gactcctacg tcgacgcgcc cttcgtcatg atggccccgg
      781 tcccggggga cgagctcgat ccgaaggtcg aggccgccta ccgcgccgag atcgaccggc
      841 actggcccga ctcgccgccg atcgccaggc tcgagcgctt cgccttctac gagcgcgcca
      901 aaaaggcctt cgccgtggtc atgacgggcg agaccgtcaa gtacggcaac atcatcgtca
      961 agaagggggt catccccctg ggcccccgga ccagatgagg ggcctagccc ctctcctttc
     1021 ccgcgtgcgg ggagaagtgc tcgcggagcc tcggcaggag ctcggtcgag gggtcgacgg
     1081 cgaaggcctc gctccgcagg agctcgccct cgcgccagga gagggtgaag cgcgggaagg
     1141 cggagccgaa gaggcggctg gccagggtcc ggtgcggctc cttcatggcc acgaggcttt
     1201 cgcgcggcac cgtgatgtcg gcgaccgccc gggccggctc cctccgcgcc gccctgaaga
     1261 ggctcgcgaa ccagttctcc gagggaaggt acttgaacct gaacgaggcg tcggtgagga
     1321 ccacgaggcc ccagacgccc cgctcgtcgc tgccgtcctc gtaccaggag ccgaccgccc
     1381 gggccacgac cttctccccc gtctcgcgct cgaactcgct ccagaatctc tcggcttcgg
     1441 cgtccatgct ggctctcctc cggtgggctg cagtatacgg ccgcccgttt cccgggtgaa
     1501 ggggctcgcg gcatgacagg gggcgagggg cagggccccg gggtcaccat ccgcgacgtg
     1561 gcgagggcag ccggagtctc gaccgcgacc gtgtcccggg tgctcaacgc cgacgcccgg
     1621 gtctcgcccg agaccgccct gcgggtccgc gcgagcgtgg acgagctggg ctacaagatg
     1681 aaccaggtgg cgaggagcct caagacgagg gccacgagga ccatcggcgt cgtggccccg
     1741 aggctcgcca gcgacttctt catgctcctg gccgagagca tggaccggga gctctcggcc
     1801 cacggctacg gcctcttcgt ctgctcctcg cgggagtcgg tgcgcgagga ggaggagcgg
     1861 ctccacctca tggccgagcg cctcgtcgac gcggtggtga tgatccccgc gaccgacagg
     1921 ggcgcccact accgcccctt catgggcagg gggaccccct tcctcctcgt ggacaggctc
     1981 gtctccgacc tcagcgccga cgccgtcctc gtggacaacg cgggcggctc gcgcgacgcg
     2041 acgagggccc tcatctccga gggccaccgg cgcatcggct tcctcggggg gagcctcgac
     2101 gtgtccacgg cgcgggagcg ctacgagggc tacagggccg cgatggccga ggcggggctc
     2161 ccggtggagc gggacttcgt gcgcttcggg agcctccacg tggagtcggg ctaccgctcc
     2221 atggccgagc tgctcgcgag gcccggggcc cccgaggcct acttcatcgt caacgccgac
     2281 acccacgtcg gggcgacgaa ctacctcatg accgagggca gggccttcag caggcggatc
     2341 gtgttcgcct cattcgacga gatgccctac tcgcccctcc tgcagttctg ccgctggtcg
     2401 gtgtcccagc ccatcgacga gctcggggcc caggcggcgc ggatggcact cgcaaggatc
     2461 ggcggctccc ggccggccga ggccgaggtc ctccgcctgc agacgaggct catcaggcac
     2521 gaggggcgag cgcacagcac agagaccgac gagatcgaat aggataagag gaggatgaca
     2581 tggcgaacgt gagcggaatg aaggtactgc cgccggcgat gcggctcaag ggcggcctgc
     2641 ccaagatcgg catcaggccg acgatcgacg gaaggctcgg gggcgtgcgg gagtcgctcg
     2701 agctgcccac catgggcatg gccagggccg ccgcgaggct catctccgag accctgaggc
     2761 acccgagcgg ccaccccgtc gagtgcgtcg tcgccgacac caccatcggg ggcgtggccg
     2821 aggccgcagc ctgcgcagag aagttcgccc gcgagggcgt ggtggccacc ctgaccgtga
     2881 ccccctgctg gtgctacggc tccgagacca tggacatgga ccccctcacc ctcaaggccg
     2941 tctggggctt caacggcacc gaccggcccg gcgccgtcta cctcgcggcc gtcctcgccg
     3001 cccacaacca gaagggcctg ccggccttcg ggatctacgg ccgcgacgtc atggacctcg
     3061 ccgacaccga caggatcccc gccgacgtcg ccgagaagat cctgcgcttc gccagggcag
     3121 ccctcgccgc cggctggatg cggggcaagt cctacctctc cgtgggctcg gtgtccatgg
     3181 gcatcgcggg atccatcgtc gacccggact tcttccaggc ctacctcggc atgcggagcg
     3241 agtacgtcga catgagcgag ctcgtgcgcc gcttcgagga gaagatctac tccgaggccg
     3301 agttccgccg cgccctggac tgggtcaagg agaactgcaa ggaggggccc gacgtcaacg
     3361 accccaaggc ccagcacagc cgcgagcgca aggacaaggc ctgggccacg agcgtgaaga
     3421 tggcgatga
//
LOCUS       DASSQV010000347         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1075427, whole genome shotgun sequence.
ACCESSION   DASSQV010000347 DASSQV010000000
VERSION     DASSQV010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075427"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFL04_06075"
     CDS             <1..264
                     /locus_tag="VFL04_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HET7839307.1"
                     /translation="DKECTLRIEMEEPTETVEIKGKIVRYENVEGRKDLTAVAIEFDE
                     SAVPMGYKLHINAFLSQVRQNRGEIDALPEQASSSEPGRRGGA"
     gene            279..1439
                     /locus_tag="VFL04_06080"
     CDS             279..1439
                     /locus_tag="VFL04_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7839308.1"
                     /translation="MELRDPEEVGATALRRLVFIELPPALARDIGSFRPDPAIPLPVE
                     TAGAATDEEGTGGRFDPRTLTGEALVAGMLRLLAWRPSHQHADYYRSFVLAVKPEILA
                     ELSQAGIAKARNKDWEIAEEIFRALAGLFPKAPEPLLDLAILSEDRAESYVETGREAL
                     AEEMAERAFEAYKRLLALEPPFPPAFFNAGFFFLRRRNFERASSLFETYIQIGDDEEK
                     KARAREIRDRLGQQGYLDNLFKEAYDFIRLGEEEKGLEKAREFIARHPEVWNGWFLVG
                     WACRRLSRWEEGREAFEKAVALGAEGVDSLNELSICLLELGRVPEARKALEKALRAEP
                     ENVKVITNLGAVALRQGRRSEAAGFFRTALELEPEDPSAKVWLERLETESQD"
     gene            1444..2538
                     /locus_tag="VFL04_06085"
     CDS             1444..2538
                     /locus_tag="VFL04_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839309.1"
                     /translation="MSPEPGSERQGPAAAVQRAAELCSFAQDLAARPRLPARSELSPQ
                     VEFVLGLLKAEDPGLRPRDSGGLPGGLVRLPAHPVVLVPDLHARPLLLAKVLAWRPDI
                     GGDARAPTGGPGQIRERASVAELLAMGEASLVCLGDAFHSEAGGAARRWRLAYREYLS
                     SWASHGAMDEEMALTLSTIRIILAAKAAFPRNFHYIKGNHDNLANEDRHGDRSFYKFA
                     AEGEMVASWFTGTYGRALLEAYRSLELELPLAVLGELLVASHGEPAFAMGPGDIIEYR
                     RREDVVYGLIWTGNDEAEAGSVQRSLAALLGPSSGGARWFGGHRPVRGRYALRAGGSY
                     VQFHDPSAHQVALILPGRPPDPERDIRILG"
     gene            2597..>2960
                     /locus_tag="VFL04_06090"
     CDS             2597..>2960
                     /locus_tag="VFL04_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839310.1"
                     /translation="MADRRRSSGASGGNPGCILWLAAFVVVLLLFVLNWGRIKGTLER
                     TNFNEIVRTQRGLDAGKPEGPAPVAPAPKVQEDPAKKGPPPTAKPAPDTEVPVGPQSP
                     QAPQPKPVDVQGARPSSPQ"
BASE COUNT          474 a         1103 c          963 g          420 t
ORIGIN      
        1 gacaaggaat gcaccctcag gatcgagatg gaggagccga ccgagaccgt ggagatcaag
       61 ggcaagatcg tccgctacga gaacgtcgaa ggcaggaagg acctcaccgc cgtggccatc
      121 gagttcgacg agagcgccgt gcccatgggc tacaagctcc acataaacgc cttcctgagc
      181 caggtgaggc agaaccgcgg cgagatcgac gccctgcccg aacaggcttc ctcctccgag
      241 cccggccgaa ggggcggggc ctgatccaag gagaaagagt ggaattgcga gaccccgagg
      301 aagtcggagc cacggcgctc cggcgtcttg ttttcatcga gcttccgccc gccctcgcgc
      361 gggacatcgg ctccttcagg cccgacccgg ccatccccct cccggtcgag acggccggcg
      421 ccgccaccga cgaggagggg acgggcggtc gcttcgaccc gaggaccctc accggcgagg
      481 ccctcgtggc cggcatgctc aggctcctgg cctggcggcc gagccatcag cacgctgact
      541 actaccgctc cttcgtcctc gcggtcaagc ccgagatcct cgccgagctc tcccaggccg
      601 ggatcgccaa ggcccgcaac aaggactggg agatagccga ggagatcttc cgcgccctcg
      661 ccgggctttt ccccaaggcc cccgagcccc tcctggacct cgccatcctc tccgaggacc
      721 gcgccgagtc ctacgtcgag acgggccgcg aggccctcgc agaggagatg gccgagcggg
      781 ccttcgaggc ctacaagcgc ctcctcgccc tcgagcctcc attccctccg gccttcttca
      841 acgcgggatt cttcttcctc cggaggcgca acttcgagcg cgcatcctcc ctcttcgaga
      901 cctacatcca gatcggcgac gacgaggaga agaaggccag ggcaagggag atccgcgacc
      961 gtctgggcca gcagggctat ctcgacaacc tcttcaagga ggcctacgac ttcatccgcc
     1021 tcggcgagga ggagaagggc ctcgagaagg ccagggagtt catcgcccgc catcccgagg
     1081 tctggaacgg ctggtttctc gtgggctggg cctgccgcag gctctcgcgc tgggaggagg
     1141 gccgcgaggc cttcgagaag gccgtggccc tcggcgccga gggcgtcgac tcgctcaacg
     1201 agctctccat ctgcctcctt gagctcggcc gggtgcccga ggccaggaag gccctcgaga
     1261 aggccctcag ggccgagccc gagaacgtga aggtcatcac caacctcggg gccgtggccc
     1321 tccgccaggg cagacgcagc gaggcggcgg gcttcttccg caccgccctc gagctcgagc
     1381 ccgaggaccc ctcggcgaag gtctggctcg agcggctcga gacggagagc caggactagg
     1441 gacgtgagcc ccgagccggg gtcggagcgg cagggcccag ccgccgccgt ccagagggcc
     1501 gccgagctct gctcattcgc ccaggacctc gccgcccgtc cccggcttcc agcgcgctcg
     1561 gaactctccc cccaggtcga gttcgtcctc ggtctgctta aggccgagga ccccgggctc
     1621 cgtccccggg attcaggcgg cctccccggc ggcctggtga ggcttccagc ccatcccgtc
     1681 gtcctcgtcc ccgacctcca cgccaggccc ctgctgctcg ccaaggtcct cgcctggcgc
     1741 cccgacatcg gtggcgatgc gcgggccccg acggggggtc cggggcagat ccgggagcgg
     1801 gcgagcgtgg ccgagctcct cgcgatgggg gaggctagcc tggtctgtct gggcgacgcc
     1861 ttccattccg aggctggcgg agccgcgagg cgttggcggc tggcctaccg cgaatacctc
     1921 tcgagctggg cgagtcatgg ggcgatggac gaggagatgg ccctcactct cagcacgatc
     1981 cgcatcatcc tcgcggccaa ggccgccttc ccccggaact tccattacat aaaggggaac
     2041 cacgacaacc tcgccaacga ggaccgccac ggcgaccgct ccttctataa attcgccgcc
     2101 gagggcgaga tggtggcctc ctggttcacc gggacctatg gccgggcctt gctcgaggcc
     2161 taccggagcc tcgagctcga gctccccctc gccgtcctcg gggagctctt agtggccagc
     2221 cacggcgagc cggccttcgc catggggccc ggggacatca tcgagtaccg ccgccgcgag
     2281 gacgtcgtct acggcctcat ctggacgggg aacgacgagg ccgaggccgg ctcggtccag
     2341 aggagcctgg ccgccctcct gggcccgtcc tcgggagggg cgaggtggtt cgggggccac
     2401 agaccggtgc ggggccgcta cgccctccgc gcgggcggca gctatgtcca gttccatgac
     2461 ccctcggccc atcaggttgc gctcatcctg cccggccgcc cccccgatcc cgagcgcgac
     2521 atcaggatcc tgggctgaag gccagatccc ccttcatatt gaccacaccc ggaccgatcc
     2581 ctatcatgtg gcccgtatgg cggatcgacg acgttcttcc ggagcctcgg gcggcaaccc
     2641 cggctgcatc ctctggctgg ccgccttcgt ggtggtgctc ctccttttcg tcctcaactg
     2701 ggggcggatc aagggcaccc tcgagcgcac caacttcaac gagatcgtcc gcacccagcg
     2761 cggcctcgac gcgggcaagc ccgagggccc ggcccccgtc gcccccgcgc ccaaggtcca
     2821 ggaggacccg gccaagaagg gcccgccccc tacggcaaaa cccgcccccg acaccgaagt
     2881 cccggtcggt ccccagagcc cccaggcccc ccagcccaag ccggtcgacg tccagggtgc
     2941 ccggccatcg agcccccagg
//
LOCUS       DASSQV010000348         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_331377, whole genome shotgun sequence.
ACCESSION   DASSQV010000348 DASSQV010000000
VERSION     DASSQV010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331377"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1970)
                     /locus_tag="VFL04_06095"
     CDS             complement(<1..1970)
                     /locus_tag="VFL04_06095"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HET7839311.1"
                     /translation="MKVRFRPELAIPAAIVLAFVLLGLFGFATSLDNRVYDLLLSLKR
                     APKEDKSLLLVDFDDKSVSKIGTWPISREIMADALMLLRECGASYAVFDIEYLNKSPR
                     GVNSEVLKVEIPENFSTEFGAIGENMKQLVDALAQGNIPLREAGSYVSDFQSQVLGGA
                     QGRLLTQVERVARDNDEYLAESIRFFGKAFLTVNLQDARDPNVPEADRRLAVETFALK
                     GLTFQGRLPPAKIEILPPIQPIAPAAKGLGFTNVVIDSDGVRRKIDLLGRYGEAWFPQ
                     LAFGPLLDWMGHPTIVATPRSITLKGARFPDGTVRDLRIPLDPEGRLIINWPHKRYGD
                     SFRHLSFLDLVLHKENLDNLVYALKNREGWGYFSLYTGEKGLLDQYREAELLREAIMT
                     GKESPDRAADFRAMRERFLSELGSFLDSRPEDKIGAAVAAVLKDPKSSKAVKDQYRTI
                     GADAPEYFKGLRTLYGELERIRKGIIAQAEGSFAVLGWTSTGSTDIGVNPFDANYANV
                     GTHAAVTNTILNRDFIDVVPAWGSALAAMILAAALVLALQNTKPIVTITAGAGATASI
                     LVIGGGFFLLTGIYMPLMTPLGATFFTFLALSATQFLKTEREKGFIRNAFSHYLSEEV
                     IKTIVGDPNKLKLGGELREMTAVFTDIKGFST"
     gene            2061..2513
                     /locus_tag="VFL04_06100"
     CDS             2061..2513
                     /locus_tag="VFL04_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839312.1"
                     /translation="MRIPLALIFAMAAAAFPLQAQSNTAVDELLGQEKAQVGSAAYMT
                     LNAGGLIGEDATPGDALAALVSKGWIAAERPVDSPILLDEYCVLVMRSLGLKGGLMYT
                     LFPGRRYGYRELVARGIVNASGGPRRTLAGDEAIRILRLALDLKGGTQ"
     gene            2510..>3327
                     /locus_tag="VFL04_06105"
     CDS             2510..>3327
                     /locus_tag="VFL04_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839313.1"
                     /translation="MRRPAATFLTILLALSFPGLLLAQEIGADLREAASFGNTENSPN
                     YAISGFSESTSLVPSFVLPMGSQAELRGAGYLQLNIPHSGSTKLLGYDLQLLKFTLAL
                     KQPKPEMDSFVLQAGRLEFADPSSYVVAAPVDALTMQFGYPGATFTFGAAYTGLLFLD
                     TSPFTISLADQARSNADTELFGSRRLLGSARLDLPRLFGQSVALSAIVQDDLNNRSDF
                     VATGSTSFDANKGGALNTQYFGLSVGGSISSLTYGTFFTYGTGRILSWLPDSLAP"
BASE COUNT          537 a         1092 c         1111 g          587 t
ORIGIN      
        1 atggtcgaga agcccttgat gtcggtgaag accgccgtca tctcccgaag ctccccgccg
       61 agcttgagct tgttcgggtc gccgacgatg gtcttgatga cctcctcgga gaggtagtgg
      121 gagaaggcgt tccggatgaa gcccttctcg cgctcggtct tgaggaactg ggtggcgctc
      181 agggcgagga aggtgaagaa ggtggcgccg agcggggtca tgaggggcat gtagatgccg
      241 gtgaggagga agaagccccc gccgatgacg aggatcgagg ccgtggcccc ggcccccgcc
      301 gtgatcgtga cgatgggctt ggtgttctgg agggccagga cgagggccgc tgcgaggatc
      361 atcgcggcga gggccgagcc ccatgccggc acgacgtcga tgaagtcgcg gttcaggatg
      421 gtgttcgtga ccgcggcatg ggtgccgacg ttggcgtagt tcgcgtcgaa ggggttcacg
      481 ccgatatcgg tggagccggt gctcgtccag ccgaggacgg cgaaggagcc ctcggcctgg
      541 gcgatgatgc cctttcggat ccgctcgagc tcgccgtaca gggtcctgag gcccttgaag
      601 tactccggcg cgtcggcgcc gatggtgcgg tactggtcct tgacggcctt cgaggatttg
      661 gggtccttga ggacggcggc caccgccgcc ccgatcttgt cctctggcct cgagtccagg
      721 aaagagccga gctccgagag gaagcgctcg cgcatcgcgc ggaaatcggc ggcccggtcg
      781 gggctctcct tgccggtcat gatcgcctcg cgcaggagct cggcttccct gtactggtcg
      841 aggaggccct tctcgcccgt gtagaggctg aaatagcccc agccttcgcg gttcttcagg
      901 gcatacacga ggttgtccag gttctccttg tggaggacca ggtcgaggaa ggagaggtgg
      961 cggaagctgt cgccgtaccg cttgtgcggc cagttgatga tgagcctgcc ctcgggatcg
     1021 agggggatgc ggaggtccct gacggtcccg tcggggaagc gcgccccctt gagggtgatc
     1081 gagcgcggcg tggcgacgat cgtcggatgg cccatccagt cgaggagggg gccgaaggcg
     1141 agctggggga accaggcctc gccgtagcgt ccgaggaggt cgatcttgcg cctcaccccg
     1201 tccgagtcga tgacgacgtt cgtgaagccg aggcccttgg ccgcgggggc gatgggctgg
     1261 atggggggca ggatctcgat cttggcgggg ggaagcctgc cctggaaggt gagacccttg
     1321 agggcgaagg tctccacggc gagcctccgg tcggcctcgg ggacgttggg gtcccgggca
     1381 tcctggaggt tgaccgtgag gaaggccttg ccgaagaagc ggatggactc ggcgaggtac
     1441 tcgtcgttgt cgcgcgcgac cctctcgacc tgggtgagga gcctgccctg ggcccccccg
     1501 agcacctggc tctggaagtc cgagacgtag ctcccggcct ctcggagggg gatgttgccc
     1561 tgggcgaggg cgtcgacgag ctgcttcatg ttttcgccta tcgcgccgaa ctcggtcgag
     1621 aagttctcgg ggatctcgac cttcagcacc tcgctgttga ccccccgggg gctcttattg
     1681 aggtactcga tatcgaacac cgcgtagctg gccccgcact cccggaggag catgagggca
     1741 tcggccatga tctccctgct gatcggccag gtgccgatct tggagacgga cttgtcgtcg
     1801 aaatccacga ggagaaggga cttgtcctcc ttgggggccc gtttgaggga caacaggagg
     1861 tcgtagacgc ggttgtcgag gctcgtggcg aagccgaaga ggccgaggag cacgaaggcc
     1921 aggacgatcg cggccgggat cgcaagttcg gggcggaatc gcactttcat gcggggctcc
     1981 ttgcgaaaac agttgaatga tgcaccggac aactatagac ttcaggagac gtcatttcca
     2041 tccaataagg agtgctcatc ttgaggattc cactagcgct gatattcgcg atggccgcgg
     2101 ccgccttccc cctgcaggcc cagtccaaca cggccgtgga cgagctgctc ggacaggaga
     2161 aggcccaggt cggctcggcc gcgtacatga ccctcaacgc cggcggcctc atcggcgagg
     2221 atgccactcc cggggacgcc cttgccgctc tggtatccaa gggctggatc gccgccgagc
     2281 gccccgtcga ttcccccatc ctgctcgacg agtactgcgt cctcgtgatg cgctcccttg
     2341 gcctcaaggg cggcctcatg tacacgctct tccccggccg ccgctacggc taccgcgagc
     2401 tcgtggcccg cggcatcgtg aacgcatcgg gcggcccgag acggaccctg gccggagacg
     2461 aggcgatacg catcctgcgc ctcgccctcg acctgaaggg aggcacccaa tgagaagacc
     2521 ggccgccacc ttcctcacga tcctcctcgc gctctccttc cctggcctgc tcctagccca
     2581 ggaaatcggg gccgacctca gggaggccgc ctccttcggc aacactgaga actcgcccaa
     2641 ctacgccatc tcgggcttct ccgagtcaac gagcctcgta cccagcttcg tgctccccat
     2701 gggcagccag gccgaactgc ggggagccgg ctacctccag ctcaacatcc cccacagcgg
     2761 cagcaccaag ctcctgggct acgacctcca gctcctcaag ttcaccctgg ccctcaagca
     2821 gcccaagccg gagatggact ccttcgtgct gcaggccggc cggctcgagt tcgccgaccc
     2881 gagctcctat gtcgtggccg ccccggtaga cgccctcacc atgcagttcg gctaccccgg
     2941 cgccaccttc accttcggcg ccgcctacac cgggctcctc ttcctcgaca cctcgccctt
     3001 cacgatctcg ctcgccgacc aggcccgctc gaacgccgac accgagctct tcggctcgcg
     3061 gcgcctccta ggctcggccc ggctcgacct cccgcggctt ttcggccaga gcgtggcact
     3121 ttcggctatc gtccaggacg acctcaacaa ccgctcggac ttcgtggcga cggggagcac
     3181 atccttcgat gccaacaagg gcggggccct gaacacccag tacttcggcc tctctgtggg
     3241 cgggtcgatc tcgtcgctga cctatggcac cttcttcacc tacggcacgg gcaggatcct
     3301 ctcatggctc cccgacagcc tggcccc
//
LOCUS       DASSQV010000349         4480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1946850, whole genome shotgun sequence.
ACCESSION   DASSQV010000349 DASSQV010000000
VERSION     DASSQV010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946850"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VFL04_06110"
     CDS             <1..1104
                     /locus_tag="VFL04_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET7839314.1"
                     /translation="IWIGQYIPLSLDVMLGMTPTNAAKIWDVFILGYCLIAAVLPMWM
                     LLQPRGHLGGYFLYAALLTAAIGIIFGGFNVQFPAFIKGFDNMGAFFFPMMPILFITV
                     ACGACSGFHSLVGSGTTSKQLKKESDAKPIGYGMMLLEGLVAVVSLACVMIVLPSDPL
                     AKKAPNFIYASGIGSFLALLGISPALGISFGLMAFTTFVYDTLDVCTRLGRYIIEELT
                     GLRNIVGKAIGTILTAGVPLIFVMQTMLDAKGNPVPAWRTFWNTFGASNQLLAALALV
                     GVTVWLINSRPLSKVWLVPFLPAVWMFSISNWALVRSVFDGWVWHTAGVHPAVPVVSL
                     VLIILSVLMAVETIVAIGTKKAKPQLSPKPAKA"
     gene            complement(1270..1584)
                     /locus_tag="VFL04_06115"
     CDS             complement(1270..1584)
                     /locus_tag="VFL04_06115"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET7839315.1"
                     /translation="MPSILLAVLVMGLVTLACRAAPFLLFMKRRPPAALDFLQRYIPP
                     MIMTILVLNGLKGIRFDRPPYGLAELSASVLVAALQLWRRNVLVSIGAGTALYMVLIR
                     VL"
     gene            complement(1577..2275)
                     /locus_tag="VFL04_06120"
     CDS             complement(1577..2275)
                     /locus_tag="VFL04_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET7839316.1"
                     /translation="MKRNGKPSSVLAAAFAASIPVLLGYVTIGIAFGLLLVRSGLPWW
                     LAPLMSVVIYAGAAQFMAVGLFAAGTGLLEIAALTLFLNARHAVYGLSLLDRFAPFRR
                     HKPYLVYALTDETYGILTTVEPPEGGHRGAFYTAVSALDQSYWVLGTAVGAIVGGLIP
                     FDTTGLDFALTALFIVLLVEQTRTVRQSLPYLVAAFSTAVSLLAIGPKNLLIASICLA
                     ILLIFPFRKRLKNA"
     gene            2467..3012
                     /locus_tag="VFL04_06125"
     CDS             2467..3012
                     /locus_tag="VFL04_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839317.1"
                     /translation="MKKVMVLVLVALACLPLVAQQYTSTTVGKVSTINNVDVTVSVDT
                     AGTIILATPTHGMYIDHGSAFSFSDTIDAIYAGMKDLDGKDLTVQDYRTLGKLTADGT
                     RERNADGIVFRLEINSIRGNKVLAVMYSTRDPEDMRFTTAAFGQLSQLVKQSLKSGAD
                     YGDQFKYIQTVVDRIGTMAFR"
     gene            3151..4254
                     /locus_tag="VFL04_06130"
     CDS             3151..4254
                     /locus_tag="VFL04_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET7839318.1"
                     /translation="MPAEDQDRDRAASGGPEPGRDGSEEALRESEWRLSRAETIARIG
                     NWKLMLGTGQMVFSRGMREIFGIDRETMSVEEVRGLDLPEYHELLDKALLDLTTKGKP
                     YDLEFRIRRARDGAIADIRTTATYDREKGTVLGVVQDITERKEAEAELLRRVKRHRLA
                     AERIEALLAEKDLVLREVHHRLKNNMLSMKSLLHLQAAKFQDAEVLAAFEDAESHLDS
                     MMLLYEQLFQAPDFSEVSVKALLPPLVAQIMANHSHGKPVQVSDSLEDIVVSAKKANA
                     LTIMVNELVTNSLKHAFADGRKGRISLTATAQDGRVTIGIGDNGVGLPEGLEPGSSPG
                     FGLVLVGELAKQLGGELHIERGGGTRVLIEFEN"
     gene            complement(4295..>4480)
                     /locus_tag="VFL04_06135"
     CDS             complement(4295..>4480)
                     /locus_tag="VFL04_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7839319.1"
                     /translation="HYERRQFSLRDIGRREYPVPADRYHQDQAKAVFRNGILRVSIPA
                     RGEGAEGGGIRIDIETE"
BASE COUNT          775 a         1502 c         1501 g          702 t
ORIGIN      
        1 atctggattg gccagtacat ccccttgagc ctcgacgtca tgctcggcat gaccccgacc
       61 aacgcggcga agatctggga cgtcttcatc ctgggctact gcctcatcgc ggccgtcctc
      121 ccgatgtgga tgctcctcca gccccgcggc cacctgggcg gctacttcct ctacgcggcc
      181 ctcctcaccg cggccatcgg catcatcttc ggcggcttca acgtgcagtt ccccgccttc
      241 atcaagggct ttgacaacat gggcgccttc ttcttcccca tgatgcccat cctcttcatc
      301 accgtcgcct gcggcgcctg ctcgggcttc cactccctcg tcggctcggg caccacctcg
      361 aagcagctca agaaggagag cgacgccaag ccgatcggct acggcatgat gctcctcgag
      421 ggcctggtgg ccgtcgtctc cctcgcctgc gtcatgatcg tccttcccag cgacccgctc
      481 gcgaagaagg ccccgaactt catctacgcc tcgggcatcg gctccttcct ggcgctcctg
      541 ggcatcagcc cagccctcgg catctccttc ggcctcatgg ccttcaccac cttcgtgtac
      601 gacaccctcg acgtctgcac ccgcctgggc cgctacatca tcgaggagct caccggcctg
      661 cggaacatcg tgggcaaggc catcggcacc atcctcacgg cgggcgtgcc cctcatcttc
      721 gtcatgcaga cgatgctcga cgccaagggc aaccccgtcc ccgcctggag gaccttctgg
      781 aacaccttcg gcgcctcgaa ccagctcctc gctgccctcg ccctcgtcgg cgtgacggtc
      841 tggctcatca acagccggcc cctcagcaag gtctggctcg tgcccttcct gccggcggtc
      901 tggatgttct cgatctccaa ctgggccctt gtccgctcgg tcttcgacgg ctgggtctgg
      961 cacacggccg gcgtccatcc cgccgtgccc gtggtgtccc tggtcctcat catcctctcc
     1021 gtcctcatgg ccgtcgagac tatcgtggcc attggcacga agaaggcgaa gccccagctg
     1081 agccccaagc cggcgaaggc ctaggcccag gccccggccc gggcgccccg agcgggctgc
     1141 ccaggggcct cacccgaaag tcgggcgcgc cctccagcca tggggggcgc gccctttttc
     1201 gtcgccgggc ggcgcgaggg cggctgcccg gcggggggcc gctcgaggat ctgtccctgg
     1261 aggctgcccc tagaggaccc tgatcagcac catgtagagg gccgtgcccg ccccgatgct
     1321 caccaggacg ttgcgcctcc agagctggag ggcggcgacg aggaccgagg ccgagagctc
     1381 ggccaggccg tagggcggcc tgtcgaaccg gatgcccttg aggccgttga gcacgaggat
     1441 ggtcatgatc atgggcggga tgtagcgctg caggaaatcc agggcggctg ggggcctgcg
     1501 cttcatgaag aggaggaatg gggccgccct gcaggcgagg gtcacgaggc ccatgacgag
     1561 gacggcgagg aggatgctag gcattcttga gcctcttcct gaaggggaag atgaggagga
     1621 tcgccaggca gatggaggcg atgaggaggt tcttggggcc gatggccagg agggagaccg
     1681 ccgtggagaa agcggcgacc agataaggca ggctctgccg caccgtcctc gtctgctcga
     1741 cgaggaggac gatgaagagg gccgtgaggg cgaagtccag gcctgtcgtg tcgaagggga
     1801 tgaggccccc cacgatggcg ccgacggccg tcccgaggac ccagtagctc tggtcgaggg
     1861 ccgagaccgc ggtgtagaag gcccccctgt gcccgccttc gggcggctcg accgtcgtca
     1921 ggatgccgta agtctcgtcg gtgagggcgt agacgaggta gggcttgtgc cgcctgaacg
     1981 gagcgaagcg gtcgaggagg gagaggccgt agaccgcgtg gcgggcgttc aggaagaggg
     2041 taagtgcggc tatctcgagg aggcccgtgc cggcggcgaa gagcccgacg gccatgaact
     2101 gggccgcccc cgcgtagatg acgacgctca tgaggggggc cagccaccag ggcaggcccg
     2161 agcggacaag gaggaggccg aaggcgatcc cgatcgtcac ataaccgagg aggacgggta
     2221 tgctggcggc gaaggccgcg gcgaggactg agcttggttt gccgtttctc ttcatcgaag
     2281 ccgaatgtag ccccaagggg ccagcgccgc aaggtgggtg cgggcggaag gccgaggcgc
     2341 cgcaggagcg cggggccgct ggcagagggg cggccttggt tttcggtgtc ccccgggcgg
     2401 ccatcgtggg tatctttcgg ggaagcacag cgtttctctt tggagaaatg cggcaaggag
     2461 agggaaatga agaaagttat ggttctcgtc ctcgtggcgc tcgcctgcct gcccctggtc
     2521 gcccagcaat acacctcgac cacagtcggg aaggtgagca ccatcaacaa cgtcgatgtg
     2581 accgtgagcg tggacacggc gggcacgatc atcctcgcca cgcccaccca cggcatgtac
     2641 atcgaccatg gttcggcctt ctccttttcg gacacgatcg acgcgatcta cgccgggatg
     2701 aaggacctcg acggcaagga cctgaccgtc caggactacc ggaccctcgg caagctcacc
     2761 gccgacggga cccgtgagag gaacgccgac gggatcgtgt tccggctcga gatcaactcg
     2821 atcaggggca acaaggtgct cgccgtgatg tactcgaccc gggatcccga ggacatgcgc
     2881 ttcacgaccg cggccttcgg ccagctgtcc cagctcgtca agcaatcgct gaagtcgggc
     2941 gcggactacg gcgaccagtt caagtacatc cagaccgtcg tcgaccggat cgggaccatg
     3001 gccttcaggt gacccgccgc ccgggcgccc cggagcccag gggcgccgcg tcggagcggc
     3061 ctgtggttgc tttccgcgga ttcgggtata caatcccctt tgaaccaata agccgtcgcc
     3121 gggcgcccaa tgtaaaaaca aggagtggcc atgcctgccg aggaccagga cagagacaga
     3181 gcagcctccg gagggcccga gcccggccgg gacggctcgg aggaggccct gcgcgagagc
     3241 gagtggcgcc tctcgcgggc agagacgatc gcgaggatcg gcaactggaa gctcatgctc
     3301 ggcacgggcc agatggtctt ctcgaggggc atgagggaga tcttcggcat cgacagggag
     3361 acgatgagcg tcgaggaggt ccgcggcctc gacctgcccg agtaccacga gctcctggac
     3421 aaggccctcc tcgacctgac caccaagggc aagccctacg acctcgagtt caggatccgc
     3481 agggcgaggg acggcgcgat agccgacatc cgcacgacgg ccacctacga ccgggagaag
     3541 ggcacggtcc tcggcgtggt ccaggacatc accgagcgca aggaggccga ggccgagctc
     3601 ctgcgcaggg tcaagcggca caggctcgcg gccgagcgca tcgaggccct cctggccgag
     3661 aaggacctcg tgctccggga ggtccaccac aggctcaaga acaacatgct ctcgatgaag
     3721 agcctcctcc accttcaggc ggcgaagttc caggacgccg aggtcctcgc cgccttcgag
     3781 gacgccgaga gccacctcga cagcatgatg ctcctctacg agcagctctt ccaggccccc
     3841 gacttctccg aggtctcggt caaggccctc ctcccgccgc tggtagccca gatcatggcg
     3901 aaccactccc acgggaagcc cgtccaggtc agcgactcgc tcgaggacat cgtggtcagc
     3961 gcgaaaaagg cgaacgccct caccatcatg gtcaacgagc tcgtgactaa cagcctcaag
     4021 cacgccttcg ccgacggccg gaagggcagg atttccctga ccgccacggc ccaagacggc
     4081 cgggtcacga tcggcatcgg cgacaacggc gtgggcctgc ccgagggcct cgagccgggg
     4141 agctcgccgg gcttcggcct ggtccttgtg ggggagcttg cgaaacagct cgggggcgag
     4201 ctgcacatcg agcggggcgg ggggaccagg gtcctcatcg agttcgagaa ctagtccccc
     4261 cgtgggcgcg aggctgcgcg ccggccgccc tgtcctactc ggtctcgatg tcgatcctga
     4321 tgcccccgcc ctcggcgccc tcgccccggg cagggatgct caccctgagg atcccgttcc
     4381 tgaagacggc cttggcctgg tcctggtggt agcggtcggc gggcaccggg tactcgcggc
     4441 ggcctatgtc gcggaggctg aactgtctgc gctcgtagtg
//
LOCUS       DASSQV010000350         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1946883, whole genome shotgun sequence.
ACCESSION   DASSQV010000350 DASSQV010000000
VERSION     DASSQV010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946883"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VFL04_06140"
     CDS             complement(<1..986)
                     /locus_tag="VFL04_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7839320.1"
                     /translation="MNFGIVGTGMIADFHARAISEMEGSRIVACLDTAAGRAGAFGAR
                     HGCAAYADMASFLAHPGLEVVNVCTPSGAHLEPALAAARAGKHLIVEKPLEIDEDRCD
                     RMIEACDRAGVALCGIFPSRFHEAPRAMKAAVDAGRFGRLTMGNAYVKWWRDQAYYDK
                     GGWKGSRALDGGGALMNQSIHAVDLLRWLMGPVDEVRAITASIGHERIEVEDCAAAVL
                     RFANGALGVVQGTTAAWPGFLKRIEVSGLDGSAVMEEENLAFWSFRRGELGDEELRSR
                     LAAGTVTGGGASDPAAIGHHGHRLQFEDMVEAIRGGRKPQVDGVEARRAVEL"
     gene            complement(983..1879)
                     /locus_tag="VFL04_06145"
     CDS             complement(983..1879)
                     /locus_tag="VFL04_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET7839321.1"
                     /translation="MRLGLFSVSYAGLWGQHALSAPAFIRKAGALGYEGVLLMAKRPH
                     LSVLDAGADELREIEAALDGAGVQLIGLAAYNDFLLKGAAELPVLEMQLSYVRECIGL
                     TRRLGGGLVRVFTGYERADEPYQRQWAGLVAALRECGRMAEDEGIVIAVQNHHDLAVD
                     SEAMARLLDEVGMPNVRAGYDAWSPALRGEELEAGALDMAPRTAMTIAADYCRLPRWR
                     YVPSLVNYERLEPDLVQAVAMGEGFIDYDGFFSALERGGFDGWAVYEMCSPLAGGPSE
                     ANLDAKAAAFVAWMRARERGRP"
BASE COUNT          303 a          730 c          672 g          296 t
ORIGIN      
        1 atcagctcga cggcccggcg ggcctcgacg ccgtcgacct ggggcttgcg ccccccccgg
       61 atcgcctcga ccatgtcctc gaactggagc ctgtggccat ggtggccgat cgccgcgggg
      121 tcggaggccc ccccacccgt caccgtcccg gccgcgagcc tcgacctcag ctcctcgtcc
      181 ccaagctccc cgcggcggaa gctccagaag gcaaggttct cctcctccat gacggcactg
      241 ccgtccaagc ccgagacctc gatgcgctta aggaagcccg gccacgccgc cgtcgtgccc
      301 tggaccacgc cgagggcccc attcgcgaaa cgcagcacgg cagccgcgca gtcctcgacc
      361 tctatgcgct cgtggccgat gctcgcggtg atcgccctga cctcgtccac cggccccatg
      421 agccagcgca ggaggtcgac ggcgtggatg gactggttca tgagggcgcc gccgccgtcc
      481 agggccctgc tccccttcca tccccccttg tcgtagtagg cctggtcgcg ccaccacttc
      541 acgtaggcgt tgcccatggt gaggcgaccg aaccggcccg cgtcgacggc ggccttcatc
      601 gcgcgcgggg cctcgtggaa gcgggagggg aagatgccgc agagcgccac gccggccctg
      661 tcgcaggcct cgatcatgcg gtcgcagcgg tcctcgtcga tctcgagggg cttctcgacg
      721 atgaggtgtt tgcccgccct cgccgcggcc agggcgggct cgagatgggc cccggacggg
      781 gtgcagacgt tgaccacctc caggccgggg tgagcgagga aggaggccat gtcggcgtag
      841 gccgcgcagc catggcgtgc gccgaaggcc cctgccctcc ccgctgccgt gtcgagacag
      901 gcgacgatcc gagagccctc catctcggag atggcgcggg cgtggaagtc cgcaatcatc
      961 cccgtgccga cgattccgaa gttcatggcc tccccctttc ccttgccctc atccatgcga
     1021 cgaaggccgc ggccttggcg tccaggttcg cctccgaggg cccgcccgcc aggggcgagc
     1081 acatctcgta gactgcccag ccatcgaagc cgccccgctc gagcgcggag aagaagccgt
     1141 catagtcgat gaagccctcg cccatggcga cggcctgcac gaggtcgggc tcgagccttt
     1201 cgtagttgac gagggagggg acatagcgcc agcggggaag ccggcagtag tcggccgcaa
     1261 tcgtcatggc tgtcctcggg gccatgtcca gggctccggc ctcgagctcc tctccgcgga
     1321 gggcgggcga ccatgcgtcg tagcccgcgc gcacgttcgg catgccgacc tcgtcgagga
     1381 ggcgcgccat ggcctcgctg tccaccgcga ggtcgtggtg gttctggacc gcgatgacga
     1441 tgccctcgtc ctcggccatc cttccgcact ccctgagcgc ggcgaccagg ccggcccatt
     1501 gccgctggta tggctcgtcg gcccgctcgt agccggtgaa gacacgcacg agccccccgc
     1561 cgagccttcg ggtgagcccg atgcactcgc ggacatagga gagctgcatc tcgaggacgg
     1621 gcagctcggc ggctcccttg aggaggaagt cgttgtaggc ggcgaggccg atgagctgta
     1681 cccccgcccc gtcgagggcg gcttcgattt cgcgcaactc gtcagcaccg gcgtcgagaa
     1741 ccgaaagatg ggggcgcttg gccatgagga gtacgccctc gtatcccagc gcacccgcct
     1801 tccggatgaa ggccggggcg ctcagggcat gctggcccca gagccccgca tagctcacgg
     1861 agaaaagccc gagcctcatc gcgacctgcc gccgagcgag gctatgccgg ccctgattcc
     1921 cgccgcgatg ccctcggtcg cgaggcgcac cggcgccgac ctgccgaaaa gagtgagggc
     1981 cacgaaggcg acgaggatca t
//
LOCUS       DASSQV010000351         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_580416, whole genome shotgun sequence.
ACCESSION   DASSQV010000351 DASSQV010000000
VERSION     DASSQV010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580416"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VFL04_06150"
     CDS             complement(<1..1145)
                     /locus_tag="VFL04_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7839322.1"
                     /translation="MINRSRATFVAFVLVLAFALAPCGAWGQMATAWSKAAALDAPDT
                     PEQLYEAAKKEGKVVVYSMSSRIKDVKAAFEKKYPGVVVEAYDMRNTEIFEKIERENA
                     AGIKNADVIFVKDSDGTMANDFYARGVVHSYVPRDLGAKVEDVFKQPGFAIYLEMKLV
                     FYNTEVYDKDPIDSWWDLTRPEWKGKIMMTNPQAAIETLGLFCSFVQNSAEMAADYKR
                     EFGQDLKLSPGVANAGYEFIKRFAANDLVITNSDQEVVDAIGAAGQKNPPIGIATSSK
                     IRDKSKGLKLGMVTTLKPRSSTGNPAYLNLVEGGQHVNAAKLLVRFMAGEVDGQAAGY
                     KPFDVEGAWPTRSDMVTKSKTPLTDMRLWPEDAKFNYPNTVKVTNYWL"
     gene            complement(1205..>2105)
                     /locus_tag="VFL04_06155"
     CDS             complement(1205..>2105)
                     /locus_tag="VFL04_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7839323.1"
                     /translation="GVNLVFQSYALWPHMTVFDNVAFGLTIRKAPRAQIREKVAGALG
                     RLRIEEYADRYPSELSGGQQQRVAIARSIVSEPKVLLLDEPLSNLDAKLRIEMRSELK
                     RLHQELKTTVVYVTHDQVEALTMSTQIAVFFEGRLVQSDSPKGIYGNPVDIRVADFIG
                     NPKINFVDCACSRDGDSMVVDSALGRIVLTEVAARNARFGGLGVEPAACGAASVLALR
                     PEHVHIGREMRPGAVKCRIYSAMPAGSETLIQAAVNGETVMAKVIGQEEFGLDEEAWL
                     SLQGDKLNVYDKRSGRIALGALV"
BASE COUNT          315 a          677 c          710 g          403 t
ORIGIN      
        1 agccagtagt tggtgacctt cacggtgttg gggtagttga acttcgcgtc ctcgggccag
       61 agcctcatgt cggtgagcgg ggtcttgctc ttcgtgacca tgtcgctccg cgtgggccag
      121 gcgccctcga cgtcgaaggg cttgtagccc gccgcctgcc cgtcgacctc gccggccatg
      181 aagcgcacga ggagcttcgc cgcgttcacg tgctggccgc cctcgaccag gttgaggtag
      241 gccgggttgc ccgtgctcga gcggggtttc agggtcgtga ccatgccgag cttgaggccc
      301 ttgctcttgt ccctgatctt cgacgaggtc gcgatgccga tgggcgggtt cttctgcccg
      361 gcggcgccga tggcgtcgac gacctcctgg tccgagttcg tgatgaccag gtcgttcgcg
      421 gcgaagcgct ttatgaactc gtagcccgcg ttggcgacgc ccgggctgag cttgaggtcc
      481 tggccgaact cccgcttgta gtcggcggcc atctcggcgg agttctgcac gaagctgcag
      541 aacaggccga gggtctcgat ggcggcctgg gggttggtca tcatgatctt gcccttccac
      601 tcgggccgtg tgaggtccca ccagctgtcg atggggtcct tgtcgtagac ctcggtgttg
      661 tagaagacga gcttcatctc gaggtagatg gcgaagccgg gctgcttgaa cacatcctcg
      721 accttggccc cgaggtcgcg cgggacatag ctgtgcacca cgccgcgggc gtagaagtcg
      781 ttggccatgg tgccgtcgga gtccttcacg aagatgacat cggcgttctt gatcccggcg
      841 gcgttctcgc gctcgatctt ctcgaagatc tcggtgttgc gcatgtcgta ggcctcgacg
      901 acgacgcccg gatacttctt ctcgaaagcc gccttcacgt ccttgatccg gctggacatg
      961 gagtagacga cgaccttgcc ctccttcttc gccgcctcgt agagctgctc cggcgtgtcg
     1021 ggggcgtcga gggctgccgc cttggaccac gctgtggcca tctggcccca ggctccacag
     1081 ggagcgaggg cgaaggcgag gaccagaacg aacgcgacga aggtcgcgcg actgcggtta
     1141 atcatgccgt acctccattt tcgggactcc aggtgattgg tataccaaac gcctcccggc
     1201 cgccctagac gagggccccg agggcaatac gcccgctccg cttgtcatag acgttgagct
     1261 tatccccctg gagggacagc caggcctcct cgtccaggcc gaactcctcc tggccgatca
     1321 ccttcgccat gaccgtctcc ccgttcacgg cggcctggat cagggtctcg gagcccgcag
     1381 gcatggccga gtagatgcgg cacttcacgg cgcccggcct catctcgcgg ccgatgtgga
     1441 cgtgctcggg cctcagggcg aggacggagg cggccccaca ggccgccggc tcgacgccca
     1501 ggcccccgaa gcgcgcgttg cgcgccgcca cttccgtcag gacgatccgg cccagggccg
     1561 agtcgaccac catcgagtcg ccgtcgcgcg agcacgcgca gtccacgaag ttgatcttgg
     1621 ggttgccgat gaagtcggcc acgcggatgt ccacagggtt cccgtagatg cccttggggc
     1681 tgtccgactg cacgagccgg ccctcgaaga acacggcgat ctgggtggac atcgtcaggg
     1741 cctcgacctg gtcgtgggtg acgtagacaa ccgtcgtctt gagctcctgg tggagccgtt
     1801 tcagctcgga gcgcatctcg atcctgagct tggcgtcgag gttcgagagg ggctcgtcga
     1861 ggaggaggac cttcggctcc gagacgatcg agcgggctat ggccacgcgc tgctgctggc
     1921 cgcccgagag ctccgagggg tagcggtcgg cgtactcctc gatgcgaagc cggccgaggg
     1981 cgccggccac cttctcccgg atctgggccc ttggcgcctt cctgatggtg aggccgaagg
     2041 ccacgttgtc gaacaccgtc atgtggggcc agagggcgta gctctggaag acgaggttca
     2101 ccccg
//
LOCUS       DASSQV010000352         3520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_208424, whole genome shotgun sequence.
ACCESSION   DASSQV010000352 DASSQV010000000
VERSION     DASSQV010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208424"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /gene="rarD"
                     /locus_tag="VFL04_06160"
     CDS             complement(<1..774)
                     /gene="rarD"
                     /locus_tag="VFL04_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HET7839324.1"
                     /translation="MRKDPSKSESRTRAPSSPERDSPKARESRTSGDHPSSERDSPKA
                     SESRESGGARAARLGTLAAFAAYGMWGLFPLYWKRLATVDPLQILAHRIVWAAVFTVA
                     LLAFSRRLRGLALVLRNPRRLAALAASAGLVTMNWGIYIWAVNSNHVTESSLGYYINP
                     LLSVALGALFLGERLDLWTRLAVGIASLGVAAASIMLGGPPWISLALAASFALYGYVK
                     KRVGLDPMTGLAAETLIAAPFALAFLIFAHASGRGSFGGS"
     gene            complement(946..1134)
                     /locus_tag="VFL04_06165"
     CDS             complement(946..1134)
                     /locus_tag="VFL04_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839325.1"
                     /translation="MSKAHRGKGLQEAVAHGRGLCPICKREGVKVVYEQEVEGQKLKI
                     CKTCKATLANKKSAAVAK"
     gene            complement(1152..1595)
                     /gene="rplQ"
                     /locus_tag="VFL04_06170"
     CDS             complement(1152..1595)
                     /gene="rplQ"
                     /locus_tag="VFL04_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET7839326.1"
                     /translation="MKHKIGFNKLNRVPAHRKALIRNMMTVLFKHERITTTRAKAIEV
                     RRFAEKEITRAKVDSVHNRRMVFRKITDEAVLAKLFTEIAPRFAQRAGGYTRILRIGE
                     RKHDAADMVILELVERKEDDKKAEREKRKAEKKAKAKAQAAAQPK"
     gene            complement(1585..2640)
                     /locus_tag="VFL04_06175"
     CDS             complement(1585..2640)
                     /locus_tag="VFL04_06175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET7839327.1"
                     /translation="MARKNLLKGFKRPKGITYEQSESGPDYGKFLAYPFEPGYATTVG
                     NTLRRILLSSIQGYAITAVRIVRYDAEGAQHIVTSEFETIPGIVEDTIEVLNELKQLR
                     IRLPEDEEQATFLYELKGPMEITGAYFAKDKPLEVLNPQIRIATLMEDARLDIEVQVD
                     LGRGYVPSEINEKYVEVVGTIPMDAIFSPVKKVKFSVEPTRVGQRSDYDKLILEIYTD
                     GTVKPEDALAEAAKIAKDHFAIFVNFDEGEAGADEESDEVEERVRQILNTPVEELELS
                     VRSSNCLKNANIKTIGDLTRKTEDDIAKTRNFGKKSLQEIKDKLKEWNLGLGMTDYAA
                     LRNYLKLSMKKEETDEA"
     gene            complement(2650..3285)
                     /gene="rpsD"
                     /locus_tag="VFL04_06180"
     CDS             complement(2650..3285)
                     /gene="rpsD"
                     /locus_tag="VFL04_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET7839328.1"
                     /translation="MARYTGPVCRLCRTEQKKMFLKGARCKSDKCPINRKRLAPGKAP
                     KSRQAKQSDYAVQLREKQKLKRSYGLMETQFRNTFERALRMPGKTGENLFGLLERRLD
                     TVVYRLRFTTSRAQGRQLVLHGHISVNGKRVNVPSYIVKQGDKIAVHPGSKKLALIKD
                     ALQEYGRSGVMPWLEVDPDNMIGTFNAIPHRQDITDMAEIREQLVVELYSK"
     gene            complement(3305..>3520)
                     /gene="rpsK"
                     /locus_tag="VFL04_06185"
     CDS             complement(3305..>3520)
                     /gene="rpsK"
                     /locus_tag="VFL04_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET7839329.1"
                     /translation="TPFAAQTVAETAVQKALTVGLREVHVYVKGPGVGRESAIRQLGA
                     LGLKVKSINDVTPIPHNGCRPRKARRI"
BASE COUNT          518 a         1089 c         1186 g          727 t
ORIGIN      
        1 gctcccgccg aagctgcccc tgcccgaggc atgggcgaag atcaggaaag cgagggcgaa
       61 gggagccgcg atgagggtct cagcggcgag cccggtcatc gggtcgagcc caacccgctt
      121 cttgacgtag ccgtacaggg cgaaggacgc cgcgagggcc agggagatcc agggtgggcc
      181 gccgagcatg atggaggccg cggccacgcc caggctggcg atcccgaccg cgagacgggt
      241 ccagaggtcc agccgctccc cgaggaaaag ggcgccaagg gcgacggaca agaggggatt
      301 gatgtagtag ccgagggagg actcggtgac gtggttgctg ttcaccgccc agatgtagat
      361 cccccagttc atggtcacca ggcccgcgct ggcggcgagg gccgcgagcc tcctcgggtt
      421 ccgcagcacg agggcgagcc cgcgcaacct tcggctgaat gcgaggaggg cgaccgtgaa
      481 gaccgccgcc cagacgatgc ggtgcgcgag gatctgcagg ggatcgactg tggcgaggcg
      541 cttccagtat agggggaaga ggccccacat gccgtaggcg gcgaaggctg cgagggtccc
      601 cagtctggcg gcgcgggcgc cgccagactc acgcgattcg cttgccttgg gcgaatcgcg
      661 ctccgaggaa ggatggtcgc cagacgtacg ggactcgcgg gcctttggcg aatcgcgctc
      721 cggggaggag ggggcccgcg tacgggactc tgattttgac gggtctttgc gcattgagcg
      781 atactaccgc cctcgcccgg actccgccta gctcgggaag ttgggcgtca aataaaaagg
      841 ccgtcccggc tttcgccagg acggcccttg gtatgcgggt aagaggggac tacttcgcga
      901 ccgccgcgct cttcttgttc gcgagggtcg ccagcgacta tcggcttatt ttgcgactgc
      961 tgccgacttc ttattggcga gagtcgcttt gcaggtcttg cagatcttga gcttctggcc
     1021 ctcgacctcc tgctcgtaga cgaccttcac gccctcgcgc ttgcagatcg ggcaaaggcc
     1081 gcggccatgc gcgaccgctt cctggagacc cttcccccgg tgtgctttgg acatgctgtg
     1141 ctcctgtagc cttacttggg ctgagccgcg gcctgggcct tggccttggc cttcttctcg
     1201 gccttgcgct tctcccgctc ggccttcttg tcgtcttcct tgcgctccac gagctcgagg
     1261 atgaccatgt cggcggcgtc gtgcttgcgc tcgccgatcc tgaggatgcg ggtgtagccg
     1321 ccggcgcgct gggcgaacct cggggcgatc tcggtgaaga gcttcgcgag gacggcctcg
     1381 tcggtgatct tgcggaagac catgcggcgg ttgtggaccg agtccacctt cgcccgggtg
     1441 atctccttct cggcgaagcg gcggacctca atggccttgg cgcgcgtcgt cgtgatgcgc
     1501 tcgtgcttga agagcacggt catcatgttc ctgatgagcg ccttgcggtg ggccgggacc
     1561 cggttgagct tgttgaatcc gatcttatgc ttcatcggtc tcttccttct tcatggacag
     1621 tttcaggtag ttccgcagcg cggcgtagtc cgtcatgccg agaccgaggt tccactcctt
     1681 gagcttgtcc ttgatctcct ggagcgactt cttcccgaag ttcctggtct tggcgatgtc
     1741 gtcctcggtc ttccgggtca ggtcgccgat cgtcttgatg ttcgcgttct tgaggcagtt
     1801 ggacgagcgc acggacagct cgagctcctc gaccggggtg ttgaggatct ggcggaccct
     1861 ctcctcgacc tcgtcgctct cctcgtcggc cccggcctcg ccctcgtcga agttcacgaa
     1921 gatggcgaag tggtccttcg cgatcttggc ggcctcggcg agcgcgtcct cgggcttcac
     1981 ggtcccgtcg gtgtagatct cgaggatcag cttgtcgtag tccgaccgct ggccgacgcg
     2041 ggtcggctcc accgagaact tcaccttctt gaccggggag aagatcgcgt ccatggggat
     2101 ggtccccacg acctcgacgt acttctcgtt gatctcggag gggacatagc cccgcccgag
     2161 gtcgacctgc acttctatgt cgagcctcgc gtcctccatg agcgtcgcga tcctgatctg
     2221 cgggttcagc acctcgaggg gcttgtcctt cgcgaagtac gcgcccgtga tctccatcgg
     2281 acccttgagc tcgtacagga aggtcgcctg ctcctcgtcc tcgggcagcc tgatccgcag
     2341 ctgcttgagc tcgttgagga cctcgatcgt gtcctcgacg atgccaggga tcgtctcaaa
     2401 ctcgctcgtg acgatgtgct gtgccccctc ggcgtcgtac ctgacgatac ggacggccgt
     2461 gatggcgtag ccctggatcg aagagaggag gatacggcgc agcgtgttgc cgacggtcgt
     2521 agcgtagcca ggttcgaaag gataggcgag gaacttgccg tagtccgggc cggactcgct
     2581 ctgctcgtac gtgatgccct tcggcctctt gaaaccttta agtaggtttt tccgcgccat
     2641 ttcggctcct tacttcgagt agagctcgac gacgagctgc tcgcggatct ctgccatgtc
     2701 ggtgatgtcc tggcgatgcg ggatcgcgtt gaacgtgccg atcatgttgt cgggatcgac
     2761 ctcgagccag ggcatgacgc cggagcgtcc gtactcctgg agcgcgtcct tgatcagggc
     2821 aagcttcttg gaaccggggt gcacggcgat cttgtcgccc tgcttcacga tgtagctggg
     2881 gacgttcacc cgcttgccgt tcacggagat gtggccgtgg aggaccagct ggcggccctg
     2941 ggcgcgcgag gtggtgaagc ggagcctgta gacgaccgtg tcgagcctgc gctcgaggag
     3001 gccgaagagg ttctcgcccg tcttgccggg catccggagc gccctctcga aggtgttccg
     3061 gaactgggtc tccatgaggc cgtagctcct cttgagcttc tgcttctcgc ggagctggac
     3121 ggcgtagtcg gactgcttgg cctgcctcga cttgggcgcc tttccgggcg cgagccgctt
     3181 gcggttgatc gggcacttgt cgctcttgca gcgagcgccc ttgaggaaca tcttcttctg
     3241 ctcggtccgg caaagccggc acacgggacc ggtgtaacgc gccattcttt gagtctcccc
     3301 ttgcctagat gcgccgggcc ttgcggggcc ggcagccgtt gtgggggatc ggcgtcacat
     3361 cgttgatgct cttgaccttg aggccgagag ccccgagctg gcggatggcc gactcgcgcc
     3421 cgacgccggg ccccttcacg tatacgtgga cctcacgaag accgaccgtg agggctttct
     3481 ggacggccgt ctcggcgaca gtctgggcgg cgaagggggt
//
LOCUS       DASSQV010000353         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_332354, whole genome shotgun sequence.
ACCESSION   DASSQV010000353 DASSQV010000000
VERSION     DASSQV010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332354"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFL04_06190"
     CDS             complement(<1..595)
                     /locus_tag="VFL04_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839330.1"
                     /translation="MLGTRAKPGSRRAWVLILGAGATAMLAIAALSSALFLSGPALGR
                     EPAPVRPGIPPEPGPEQGPIPALEGPVTIAIQPGHWQVAELPDELWRLRESTGAVWGG
                     TREVDINRGVAVALSGLIQARGWRALVLPATIPPGLRADAFIAIHADWGPSPGIRGWK
                     VAPPWRASAASERLADELGASFAAEPGSLEDRGGITVS"
     gene            665..1240
                     /locus_tag="VFL04_06195"
     CDS             665..1240
                     /locus_tag="VFL04_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7839331.1"
                     /translation="MTQKDEREDLALLRRIADSESGALRALYQKYGSLVYGIALRVTG
                     DGAVAEEVAQDVFMNAWRSASTYRPELGAVATWLGRIARNRAVDALRSQRARGSLARD
                     DWTEAAEQEDPRSRGPEEETARSIRAGEVREAVAALPEPQRQALSLAFFRGLSHSEIA
                     AALSLPLGTVKSRIRDAMRSLRGRLGEGGGK"
     gene            1311..1985
                     /locus_tag="VFL04_06200"
     CDS             1311..1985
                     /locus_tag="VFL04_06200"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET7839332.1"
                     /translation="MTDHGEIADLLPGLALGCLDPEEESLAREHLAGCAQCRAELASM
                     RDVVGELAVTVPCQEPPPGLEARIMAGLPRSRSPRRATFLLAAAAVLIVVLAAGNLVQ
                     WQRSQTGLSRRDAGGLLTIALAGSGASAAAFGTVVLDPEDNEGVLAVRGLGALDASRA
                     YQLWLLKGERRASGGVFVVDEHGYGSLLLEVPSEFRGFDAFLVTSEPKGGSPSPTGSP
                     VMKGRR"
     gene            2091..>2376
                     /locus_tag="VFL04_06205"
     CDS             2091..>2376
                     /locus_tag="VFL04_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7839333.1"
                     /translation="MSKRFHLALGVLILAASTSFALGTGGGGSGGTSSGASMSAPPKT
                     ALQFYNAGYSASQAGRYDEAVSDFRQAISLKADYAEAYNMLGFSLRKSGNV"
BASE COUNT          356 a          869 c          840 g          311 t
ORIGIN      
        1 tcgacacggt gatgcccccc ctgtcctcaa ggctcccggg ctcggcggcg aagctcgccc
       61 ccagctcgtc agcgagccgc tcgctggccg ccgaggccct ccatggcggg gcgaccttcc
      121 agcccctgat gcccgggctg gggccccagt ccgcgtggat ggcgatgaag gcgtcggcgc
      181 ggagcccggg cgggatcgtg gcgggcagga ccagggccct ccagcccctg gcctggatga
      241 ggccggagag ggccaccgcc acgccccggt tgatgtcgac ctcccttgtg cctccccaga
      301 ccgcgcccgt gctctccctg agcctccaga gctcgtcggg cagctcggcc acctgccagt
      361 gtccgggctg gatcgcgatg gtgaccggac cctcgagggc cgggatgggc ccctgctcgg
      421 gaccgggctc gggcgggatg ccgggccgga ccggggccgg ctcgcggcca agggcggggc
      481 cggacaggaa cagggctgag gagagggcgg cgatggccag catggcggtg gccccggccc
      541 cgaggatgag gacccatgcg cggcgggagc ccggcttcgc ccttgttccc aacatggacc
      601 aaagatatga cacggcggcc gatatggaca gcggccgcgg gggaacctag actgcaagcc
      661 acgcatgacg caaaaagacg aacgcgagga cctggccctc ctgcgccgca tcgccgactc
      721 tgagagcggg gccctcaggg ccctgtacca gaagtacggc agcctcgtct acggcatcgc
      781 cctccgcgtg acgggggacg gggccgtggc cgaggaggtg gcccaggacg tcttcatgaa
      841 cgcctggcgc agcgcctcca cctacaggcc cgagctcggg gccgtggcga cctggctcgg
      901 ccggatagcg cgcaaccgcg ccgtggatgc cctgcggagc cagagggcca ggggctccct
      961 cgcccgggac gactggaccg aggccgccga gcaggaggac ccgaggagcc ggggacccga
     1021 ggaggagacc gcgcgctcca tcagggccgg agaagtccgc gaggcggtgg ctgccctccc
     1081 cgagccccag cgtcaggccc tctcgctggc ctttttccgg ggcctgagcc actccgagat
     1141 cgctgcggcc ctgtccctgc ccctcggcac cgtcaagtcg aggatcaggg acgccatgcg
     1201 gagcctcaga gggcggcttg gcgaaggtgg gggcaaatga gcatccgtgc gccctcgcgc
     1261 cgcgtatata cgctggatgg aggtccgcgc cgcggaccgg aggatggaca gtgacagacc
     1321 atggagagat agccgacctc cttccaggcc ttgccctcgg ctgcctcgat cccgaggagg
     1381 agagcctggc gcgcgagcac ctcgccggct gcgcccagtg ccgcgccgag ctcgcctcga
     1441 tgcgcgatgt ggtcggggaa cttgcggtga ctgtcccctg ccaggagccg ccccccggcc
     1501 tcgaggcccg gatcatggcc ggcctgcccc ggagccgaag cccgcgcagg gccaccttcc
     1561 tcctcgccgc cgcggccgtc ctcatcgtgg tcctcgcagc gggcaacctc gtccaatggc
     1621 agcgctccca gacgggcctt tcccgccgcg acgcgggcgg cctcctcacc atcgccctcg
     1681 cgggctcggg agcgagcgca gccgccttcg gcaccgtcgt cctcgacccc gaggacaacg
     1741 agggcgtcct ggccgtcagg ggcctcggcg ccctggacgc gagccgggcc taccagctct
     1801 ggctcctcaa gggcgagcga agggcctcgg gcggggtctt cgtcgtcgac gagcacggct
     1861 acggctcgct tctcctcgag gtcccctccg agttcagggg cttcgacgcc ttcctcgtga
     1921 ccagcgagcc caagggaggg agcccctccc ccaccggaag cccggtcatg aaggggaggc
     1981 gctagactcc catccgggcg gctccacccc gcgtatattc ggggcaaggg cgaggcgatc
     2041 ggcatcacgc gtcgcgcaag cccgcccccg accgggggaa aggagccata atgtccaagc
     2101 gtttccatct cgccctcggc gtcctcatac tcgccgcgtc aacgagcttc gccctcggca
     2161 cgggcggcgg aggctcgggc gggacctcgt cgggcgcctc gatgagcgcg ccaccgaaga
     2221 cggccctcca gttctacaat gccggctaca gcgcctccca ggcggggcgc tacgacgaag
     2281 ccgtcagcga cttcaggcag gcgatctcgc tcaaggccga ctacgccgag gcctacaaca
     2341 tgctgggctt cagccttagg aagtcgggca acgtga
//
LOCUS       DASSQV010000354         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_332938, whole genome shotgun sequence.
ACCESSION   DASSQV010000354 DASSQV010000000
VERSION     DASSQV010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332938"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFL04_06210"
     CDS             complement(<1..221)
                     /locus_tag="VFL04_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839334.1"
                     /translation="MSMADQESVYVLLSNAYESLRAVDLAGASELLERALAMDFEHPE
                     VLWSLKCVNFWVERLARLEATPNPYERGE"
     gene            353..1033
                     /gene="rpe"
                     /locus_tag="VFL04_06215"
     CDS             353..1033
                     /gene="rpe"
                     /locus_tag="VFL04_06215"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET7839335.1"
                     /translation="MGKPIIAPSLLSADFSDMRSGLARIADSGADWVHLDVMDGRFVP
                     SITFGHKMAADLRPHTSLPLDVHLMTVEPERLVQGFVEAGADWITFHIEACVHAHRLA
                     QTLRSAGVHPGISIVPSTPLALIEELLPHVDLVLVMTVNPGFGGQSLIPSCLDKVRRL
                     VELRRREGLGYLISVDGGVNRGTMAEVAAAGPDVLVMGSAFFSSDNSSAEVEAARRAF
                     AGSAQGPV"
     gene            1070..>2007
                     /locus_tag="VFL04_06220"
     CDS             1070..>2007
                     /locus_tag="VFL04_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7839336.1"
                     /translation="MPMHRSPIILLVLLVAASVAGAQQTPAPRPTASPPAAQPTAPQA
                     EDSKATLARGIDLFGQGRFREALDLFGRILADPRAAADRPEAAYWSALAYISSGDSVN
                     ASSSIDAFLSAYPPSGRVPDLLYQKGRLLYLKQDYEGALRSFAAFADVAPSSDLMPSA
                     LYWSGECLYALGRLEEAERAFAAVVEKYPSSVKVEAASYRRSLIGLEYRERELIRLLT
                     WSHEESLRAADDFRRRERAYEQSVSIYQKQIADLKRGAPSDQEKTIADLRSQVADLGS
                     KLASSDAQLSSTKAELDALRAAQAAEAAAKAQGGAAP"
BASE COUNT          306 a          746 c          627 g          328 t
ORIGIN      
        1 tactctccgc gctcataggg gttgggcgtg gcctcgaggc gagcgaggcg ctcgacccag
       61 aaattgacgc acttcagtga ccagagcacc tcggggtgct cgaagtccat ggcgagcgcc
      121 cgctccagca gctccgacgc accggcgagg tcgactgccc tgaggctctc atacgcgttc
      181 gacaaaagga cgtaaacgct ttcctggtcg gccatgctca tccgggtatt cagggtaata
      241 taggactgtt cgatactacc cgatagcgcc ccggtagtca atcagacgcg ggaaacggcc
      301 gcgcgccgcc gatggcgaca ccgcggcggc cttgtgctag aatccatcca ccatgggcaa
      361 gccgataatc gcaccctccc tgctctcggc ggacttctcc gacatgcgtt cgggccttgc
      421 gcgcatcgcg gattcgggcg ccgactgggt ccaccttgac gtgatggacg gccgtttcgt
      481 ccccagcatc accttcgggc acaagatggc cgccgacctc aggccccata cctcccttcc
      541 cctcgacgtc cacctcatga cggtcgagcc cgagcgcctc gtccagggct tcgtggaggc
      601 gggcgctgac tggatcacct tccacatcga ggcctgcgtc cacgcgcatc gccttgcgca
      661 gaccctccgg tcggcggggg tccatcccgg catctccatc gtgccctcga cccccctggc
      721 cctcatcgag gagctcctcc cccatgtcga cctcgtcctc gtgatgaccg tcaatcccgg
      781 cttcggcggg cagagcctga taccctcctg tctggacaag gtgcgccgcc tcgtcgagct
      841 caggcggagg gagggcctcg gctatctcat ctcggtggac gggggcgtga accgggggac
      901 catggccgag gtggcggcgg ccggccccga cgtcctcgtg atgggaagcg cattcttctc
      961 ctcggacaat agctcggccg aggtcgaggc ggcgcggagg gccttcgccg ggtccgccca
     1021 gggcccggtt tgagttttcc ggccgaggcg ccgataatca atcggaggca tgcccatgca
     1081 taggtccccc atcatcctcc tcgtcctcct tgtcgcggcc tcggtagcgg gcgcgcagca
     1141 gacgcccgcc cccaggccga cggcttcgcc tccggcggct cagccgacgg cgccccaggc
     1201 ggaagactcc aaggcgaccc tcgctcgggg catcgacctc ttcggacagg ggcgcttccg
     1261 cgaggccctc gaccttttcg ggcggatcct ggccgatccg cgggcggccg cggacaggcc
     1321 cgaggcggcc tactggtcgg ccctcgccta catctcctcg ggcgactcgg tcaacgcctc
     1381 ctcctcgatc gacgccttcc tctccgccta tcccccgagc ggcagggtgc ccgacctcct
     1441 ttatcagaag ggcaggctcc tctacctgaa gcaggactac gagggggcgc tccgctcctt
     1501 cgcggccttc gccgacgtcg ctccctcgag cgacctcatg ccttcggccc tctattggag
     1561 cggggaatgc ctctacgccc tcggccgcct cgaggaggcc gagcgggcct tcgccgccgt
     1621 cgtcgagaag tatccctcga gcgtcaaggt cgaggccgcc tcctaccgcc gctccctcat
     1681 cggcctcgaa taccgggaga gggagctcat caggctcctg acctggagcc acgaggaatc
     1741 cctcagggcg gccgacgact tcaggaggcg ggagcgggcc tacgagcagt cggtctccat
     1801 ctaccagaag cagatcgcgg acctcaagcg gggcgccccc tccgaccagg agaagaccat
     1861 cgccgatctc cgctcccagg tcgccgacct gggctcgaag ctcgcgagct cggacgccca
     1921 gctctcgtcc accaaggccg agctcgatgc cctgcgcgcc gcccaggccg ccgaggccgc
     1981 ggcgaaggcc cagggcggag ccgcgcc
//
LOCUS       DASSQV010000355         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1576599, whole genome shotgun sequence.
ACCESSION   DASSQV010000355 DASSQV010000000
VERSION     DASSQV010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576599"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFL04_06225"
     CDS             <1..327
                     /locus_tag="VFL04_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7839337.1"
                     /translation="CGACTVLLDGRPVKSCMVLAVEAIGSAVTTIEGIGDSVMVRAFL
                     EENGFQCGFCTPGMILNAHALLLAHPEPTEEQKREWMESNLCRCTGYEGIGRAIQRAL
                     ALRRSP"
     gene            complement(294..578)
                     /locus_tag="VFL04_06230"
     CDS             complement(294..578)
                     /locus_tag="VFL04_06230"
                     /inference="COORDINATES: protein motif:HMM:NF040785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HET7839338.1"
                     /translation="MSYIKADDVLPSELLREVQKYVQGSLVYVPNPESGRRAWGERNG
                     AREAFDRRNQEIREAKARGRRLEDLAEDYGLSVDAIRKVLYGDRRRARAR"
     gene            1110..>2240
                     /locus_tag="VFL04_06235"
     CDS             1110..>2240
                     /locus_tag="VFL04_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7839339.1"
                     /translation="MPADDRELPSGTVTLLFTDIEGSTRILQSLGAGYALLLEEHRRI
                     LSGIVEARGGRIVDSQGDSCFASFPGAVAAVAAAAEAQLAFAAKTWPGGVELRVRMGM
                     HTGEPSLSGGAYVGIDVHKAARIGAAAHGGQVLLSETTKTLVEPALPPGLSLSDLGEH
                     ELKDIPRRTRLYELVVEGLRGGFPPPRTYRPGSSSLPAMLSSFVGRERERRELGRSVM
                     DHRLVTLMGPGGTGKTRLSLVVAAELLEAGAFPDGAFFVELGMITDPELVPRATAMAL
                     GLTLNSGLPALERLADFLRRKELLIILDNCEHLVEACASLASGLLGATRELRILATSR
                     ESLGIEGETVWRLPSLSVPAPGLSGVAAVSAFESVRLFVDRAA"
BASE COUNT          336 a          796 c          738 g          370 t
ORIGIN      
        1 tgcggagcct gcaccgtcct cctggacggg aggccggtca agtcctgcat ggtcctcgcc
       61 gtcgaggcga taggctcggc cgtgacgacg atcgagggca tcggcgactc ggtcatggtc
      121 agggccttcc tcgaggagaa cggcttccag tgcggcttct gcacgcccgg catgatcctc
      181 aacgcccacg ccctcctcct ggcccatccc gagccgaccg aggagcagaa gcgcgaatgg
      241 atggagtcca acctctgccg ctgcacgggc tacgagggga tcggccgggc gattcagcgc
      301 gccctcgccc ttcggcggtc gccgtagagg accttcctga tcgcgtcgac gctgaggccg
      361 tagtcctcgg ccaggtcctc gagacggcgg cccctggcct tggcctccct gatctcctgg
      421 ttgcggcggt caaaggcctc tcgggccccg ttcctctctc cccaggcccg ccttcccgac
      481 tcggggttgg ggacatagac gagggaaccc tggacgtatt tctggacttc tctcaaaagc
      541 tcggacggca ggacatcgtc cgccttgatg tatgacatga accttcctca tggaatgcct
      601 cgggcgcttc cgccgccttg agggcctgtt cgtgaggagg gccgaaaccg gtggtcggct
      661 gggggacagg cttcttcagg cggcgccgaa agacggggaa acgggctctt tgtgcacaat
      721 catcattgcg tgcctcctgt cgatgaagct gtgaagccat catgccacag agccgggccc
      781 tgtgcaaatg gtagagaacg agtgacacgc aatctctcgc tttacagccc agccgctccg
      841 atgcgatgct cgtctcctgc cggcatgaac gcgcgcggtc cgtccctggc ccggacggga
      901 aggaggcagc agttgactga acctacgcag agggcgaagg ccctctaaaa gccaccccgt
      961 ggagaacacg gaccggggcg ggggccggac cgcggagacg cggcgaggcc cccgcctctt
     1021 gttacccctg cctcgtacct tccccgccca cgttgacccc gccagccctt gcccctacac
     1081 tgatcgggaa gcaaggagga cgctgcggca tgccagctga cgatcgagag ctgccctcgg
     1141 gaacggtgac cctcctcttc accgacatcg agggctccac gcggatcctc cagagcctcg
     1201 gcgcgggcta cgccctcctg ctcgaggagc accgccgcat cctttcgggc atcgtcgaag
     1261 cccggggcgg gaggatcgtg gacagccagg gcgactcctg tttcgcctcc ttccccgggg
     1321 ccgtcgcggc cgtggcggcc gcggccgagg cccagctcgc cttcgccgcg aagacctggc
     1381 cggggggggt ggagctcagg gtcaggatgg gcatgcatac gggggagccc tctttgtcgg
     1441 ggggagccta cgtcggcatc gatgtccaca aggccgcgag gatcggggct gccgcccacg
     1501 ggggccaggt cctcctctcg gagaccacga agaccctcgt cgagccggcc ctgccgcccg
     1561 gcctctccct gagcgacctc ggggagcacg agctcaagga catcccgcgg cgcaccaggc
     1621 tctacgagct tgtcgtggag gggctccggg ggggcttccc ccctccccgc acgtaccggc
     1681 ccggctcctc gagcctcccc gccatgctca gctccttcgt cgggagggag cgggagcgcc
     1741 gcgagctcgg ccgtagcgtc atggaccacc gcctcgtcac cctcatgggt cctgggggga
     1801 cgggcaagac caggctctcc ctcgtcgtgg ccgccgagct gctcgaggcc ggagcctttc
     1861 ccgacggggc cttcttcgtc gagctcggca tgatcaccga tcccgagctc gtgccgcggg
     1921 ccacggcaat ggccctcggg ctcaccctca actcgggcct ccccgccctg gagcggctcg
     1981 cggatttcct gaggcgcaag gagctcctca tcatccttga caactgcgaa catctggtcg
     2041 aggcctgcgc ctccctggcc tcgggcctgc tcggggccac gagggagctc cgcatcctcg
     2101 ccacctcaag ggaatccctc gggatcgagg gcgagacagt ctggcgcctg ccctcattga
     2161 gcgtgccggc tccaggcctc agcggggtcg cggcagtctc ggccttcgag tcggtcaggc
     2221 tcttcgtcga cagggcagcc
//
LOCUS       DASSQV010000356         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2323116, whole genome shotgun sequence.
ACCESSION   DASSQV010000356 DASSQV010000000
VERSION     DASSQV010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323116"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFL04_06240"
     CDS             complement(<1..372)
                     /locus_tag="VFL04_06240"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HET7839340.1"
                     /translation="MLSFIDAYTAALIAVAILSLFLASRAFSWPANGAREFGCIQIGI
                     AVYAIGYVAELSSTSLLGAMRAVRFEFLGIAALLIAWVLFALRFSGRSRIRPALVALI
                     SFLPACTVVMVWTDPLHHLFYA"
     gene            complement(392..1729)
                     /locus_tag="VFL04_06245"
     CDS             complement(392..1729)
                     /locus_tag="VFL04_06245"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET7839341.1"
                     /translation="MVILTLNCGSSSVKYQVYDWEKKDVLATGIIERVTLGGSSITHK
                     AKGKADYVVTHECPTHTVAIELILNTLVHPEYGVIKDLSVIKAVGHRMVHGGMKFARS
                     VLIDDEFLATFRELNDLAPLHNPANLMGVEAAKAVLPGVQQCAIMDTAWHQTMPAWAY
                     MYPVPREWYEKFMVRRYGFHGTSFLYNAKRAAAILGQDPFKTNIITAHIGNGASINAV
                     KDGCSYDTSMGLTPLEGLVMGTRSGDIDPGIIFHMMRKAGLHASDVEKKLNKESGVLG
                     ITGRWADRRDIEQAAEKGDEIALLAQQLEGYRIKKYIGAYYAALGRVDALVFTAGVGE
                     MGPVTRQLATAGLENLGIVIDEKKNSLAKCRNAELEITGAGSKVRVFVIPTDEELVMT
                     EDSFALISGTYDVHTKYHYTFQDCDYINKAREEGLKRDLEKKPWLKDIIVRPC"
     gene            complement(1917..>2215)
                     /locus_tag="VFL04_06250"
     CDS             complement(1917..>2215)
                     /locus_tag="VFL04_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839342.1"
                     /translation="LLADASAILGGMLKDEAGLAVTMKSTGAPSADKGFTVQDFSGEA
                     TRGEGEHAFLRARLFISAGSVHAMVALVKDQADLGADSEAASILDSVELSSATK"
BASE COUNT          343 a          651 c          828 g          393 t
ORIGIN      
        1 ggcgtagaat aggtggtgga gggggtcggt ccacaccatc acgacggtgc aggcgggaag
       61 gaaggagatc agggccacga gggccggcct gatccggctg cggcccgaga agcggagggc
      121 gaagaggacc caggcgatca gaagggcggc gatgccgagg aactcgaagc gcaccgcgcg
      181 catcgcgccg aggaggctcg tcgaggagag ctcggccacg tagccgatgg cgtagaccgc
      241 gatcccgatc tggatgcagc cgaactcgcg ggcgccgttg gcgggccagg agaaggcccg
      301 ggaggcgagg aagagggaga ggatcgcgac ggcgatcagc gccgccgtgt aggcgtcgat
      361 gaagctcagg atcccctccc cccgacgggc gtcagcaggg cctgacgatg atgtctttca
      421 gccagggctt cttctcgagg tcccgcttga gaccctcctc cctggccttg ttgatgtagt
      481 cgcagtcctg gaaggtgtag tggtacttcg tgtgcacgtc ataggtgccc gagatcaggg
      541 cgaagctgtc ctcggtcatg acgagctcct cgtcggtggg gatcacgaag acccgcacct
      601 tggagccggc gcccgtgatc tcgagctcgg cgttgcggca cttggcgagg gagttcttct
      661 tctcgtcgat gacgatgccg aggttctcca gcccggccgt cgccaactgg cgggtcacgg
      721 ggcccatctc gccgacgccg gcggtgaaga cgagggcgtc gacccggccc agggcggcgt
      781 agtaggcgcc gatgtacttc ttgatccggt agccctcgag ctgctgggcc aggagggcga
      841 tctcgtcgcc cttctcggcg gcctgctcga tgtcgcggcg gtcggcccag cggccggtga
      901 tcccgaggac ccccgactcc ttgttgagct tcttctcgac gtcggaggcg tggaggccgg
      961 ccttgcgcat catgtggaag atgatgccgg ggtcgatgtc gcccgagcgg gtgcccatga
     1021 cgaggccctc gaggggggtg aggcccatcg aggtgtcgta ggagcagccg tccttgacgg
     1081 cgttgatgga ggcgccgttg ccgatgtggg cggtgatgat gttggtcttg aaggggtcct
     1141 ggcccaggat ggccgcggcg cgctttgcat tgtagaggaa gctcgtgccg tggaagccgt
     1201 agcggcggac catgaacttc tcgtaccact cgcggggcac ggggtacatg taggcccagg
     1261 cgggcatggt ctggtgccag gccgtgtcca tgatggcgca ctgctggacc ccgggcagga
     1321 cggccttggc cgcctcgacg cccatgagat tggcggggtt gtggaggggg gccaggtcgt
     1381 tgagctcgcg gaaggtggcg aggaactcgt cgtcgatgag gaccgagcgg gcgaacttca
     1441 tgccgccgtg gaccatgcgg tggccgacgg ccttgatgac cgagaggtcc ttgatgaccc
     1501 cgtactcggg gtggaccaag gtgttgagga tcagctcgat ggcgaccgtg tgggtggggc
     1561 actcgtgggt gaccacgtag tcggccttgc ccttggcctt gtgggtgatg gaggagccgc
     1621 cgagggtgac ccgctcgatg atgcccgtcg cgaggacgtc cttcttctcc cagtcgtaga
     1681 cctggtattt gaccgatgat gagccgcagt tgagggtgag gatgaccatg gattctcctt
     1741 aggctgtttc gtcggggtca tcgaaggatg gtcggccccg gcctcgcgcc gcgcgccccg
     1801 ccttgggacg gacgcacggc gaggcattct agcacctgtt ccggcgattg ggcaattcat
     1861 tgtgtgctaa atgacaaaag gcccgatcct gtcggactcg ggcccctcag ccggccctat
     1921 ttcgtggccg aggatagctc gacggagtcg aggatcgagg ctgcctccga gtccgcgccg
     1981 aggtcggcct ggtccttcac gagggccacc atcgcgtgga ccgagcccgc gctgatgaag
     2041 agcctggccc tgaggaaggc gtgctcgccc tcgccgcggg tggcctcgcc cgagaagtcc
     2101 tggaccgtga agcccttgtc ggcgctcggc gcccctgtgc tcttcatggt gacggcgagc
     2161 ccggcctcgt ccttgagcat gccgcccagg atggcggagg cgtcggcgag gaggc
//
LOCUS       DASSQV010000357         3918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1077609, whole genome shotgun sequence.
ACCESSION   DASSQV010000357 DASSQV010000000
VERSION     DASSQV010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077609"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFL04_06255"
     CDS             complement(<1..459)
                     /locus_tag="VFL04_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7839343.1"
                     /translation="MRASETRRPRGPGLLALALVLSASLLGCAKPPAKAPAPPGGDAA
                     FKVAILLPRSIEADGWTRQGYKGLKLIEAQLGAQVAYAENLSETDFEKTFRKYAEEGY
                     DFIIGHGAQFVAAAEKAAEAYPRTSFAVAGVYGGNNRNLGAISLREGEMAY"
     gene            complement(446..3175)
                     /locus_tag="VFL04_06260"
     CDS             complement(446..3175)
                     /locus_tag="VFL04_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7839344.1"
                     /translation="MYGKRVLSRVRLQVLSLALSLLILALSPPASIAEAPGGRHALVI
                     GNASYKGLPALRNPVNDATDLAAALKGLGFSVQLLTDATRKQMNQALVAFRETLAKDP
                     FSEGLFFFAGHGIQHEGINYLLPLGSEIRTAADLEDEALSAQKVLASLAEAHNRVNLV
                     ILDACRDNPLPSALRGAMRGLAVVAAAPPETVVLYSTGAGQSAQDGDGRNSPFAAALL
                     KFIAEPGDITRTVKLVTAEVKAATKGAQTPYQYSSLDLDFELNPGGRALAAKPAPGFS
                     EAEAEVFRSGELAAVRRLLASGIKADARDEEGVSPLLWASLANPHTEVIKALLEAGAR
                     PDYGAELGATALVAAAIGGQNPATIRLLLSSGSRIEEKVADGGTALMAAASSGKAETV
                     KLLLSLGARLDERDKDGKTALARAALGLCDPLCIKALVAAGARFSAQDSERLAPLVGA
                     ARTAGPEALKALMAAGAKPGERDAEGTSPLLAAAGSNEDPAVIRLLLASGSSLAEKNG
                     AGETALFAALSGNQNPEVLKAILAAGAKATERGAEGQSPIMAALRSEKAAEKVRILAA
                     AGARVDQRDEGEEPPLFYAARYCDGAAVKALLDAGAKPGTRIEDGLSPLIAAASSTEH
                     PEVIGVLLKAGAKIGERKEDGTTALLEAAAFNESLEVLQTLLAAGAKIGDTNDSGWTA
                     LMHAAAFNPNEAMIPALIHAGAKVGEATEDGWTALFSSAYHNDKPAVTLALLDAGERI
                     DARSSSLSTPLIEAAGGNPNPEVIKVLLGAGAKIDERNDEGWTPLISAACYSSYPEII
                     ACLLEAGSKIDERDKAGWSPLLVAAKWQGPEIIKALLAAGAKPSERTREGLTPLMVAA
                     ANNEDPEVIRLLLAAGSNPKARTAKGETALDFIKQNETLKASDAYWELNDASQ"
     gene            3351..>3918
                     /locus_tag="VFL04_06265"
     CDS             3351..>3918
                     /locus_tag="VFL04_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HET7839345.1"
                     /translation="MTRSRSLRVLLLPLGLGLGLLSCTNPSGWMAAIWPPRMASTLAT
                     PVIGLNSGIYKAAISVPIGGPAGATLLYTLDGTDPTPTSPVYAGPVPIAPPASQAGQG
                     LFVLKVRASQSGWTDSAVASATFIVDPWMKKYIGSGPDSLSDGIAAVLVALNGPDGIC
                     LNAAGDLYVADTSNDVVRVVSHGSTLTYF"
BASE COUNT          488 a         1310 c         1479 g          641 t
ORIGIN      
        1 gtaggccatc tcgccctcgc ggagcgagat cgccccgagg ttcctgttgt tgccgccgta
       61 gaccccggcc acggcgaagg aggtcctcgg gtaggcctcg gcggccttct ccgccgccgc
      121 cacgaactgg gccccgtggc cgatgatgaa gtcgtagccc tcctcggcgt atttcctgaa
      181 ggtcttctcg aagtcggtct cgctcaggtt ctcggcgtag gcgacctggg cgccgagctg
      241 ggcctcgatg agcttgaggc ccttgtagcc ctggcgggtc catccgtcgg cctcgatgct
      301 gcggggcagg aggatggcca ccttgaaggc ggcgtcgccg cccggcgggg ccggggcctt
      361 ggcgggcggc ttcgcgcagc caaggaggga ggcggagagg accagggcca aggccaggag
      421 gccagggccg cgaggccgcc tcgtctcact ggctcgcatc gttgagctcc caatatgcgt
      481 cgctggcctt gagcgtctcg ttctgcttta tgaagtcgag ggcggtctcg cccttcgcgg
      541 tcctggcctt cgggttggat ccggcggcga ggaggagcct gatcacctcg gggtcctcgt
      601 tgttggcagc ggccaccatg aggggggtca ggccttccct cgtgcgctcg ctgggcttgg
      661 cgccggccgc gaggagggcc ttgatgatct cggggccctg ccacttggcg gccacgagga
      721 gggggctcca gccggccttg tcccgctcgt cgatcttgga gcccgcctcg aggaggcagg
      781 cgatgatctc ggggtaggag ctgtagcagg cggcgctgat gaggggggtc cagccctcat
      841 cgttgcgctc gtcgatcttc gcccccgcgc ccaggaggac cttgatgacc tcggggttag
      901 ggttgccgcc cgcggcctcg atgaggggag tggagaggct cgagctgcgc gcgtcgatcc
      961 tctcgccagc gtcgaggagg gcgagggtga ccgcgggctt gtcgttgtgg taggcggagc
     1021 tgaagagggc ggtccagccg tcctcggtcg cctcgcccac cttggccccc gcgtgtatga
     1081 gggcggggat catggcctcg ttggggttga aggcggccgc gtgcatgagg gcggtccagc
     1141 ccgagtcgtt ggtgtcgcct atcttcgccc ccgcggccag gagggtctgg aggacctcga
     1201 ggctctcgtt gaaggcggcg gcctcgagga gggccgtcgt gccgtcctcc ttgcgctcgc
     1261 cgatcttcgc gcccgccttg aggaggaccc cgatcacctc ggggtgttcg gtcgacgagg
     1321 ccgcggcgat gaggggcgag agcccgtcct cgatcctcgt gccgggcttg gccccggcat
     1381 caaggagggc cttgacggcg gcgccgtcgc agtagcgcgc ggcgtagaag aggggcggct
     1441 cctcgccctc gtccctctgg tcgaccctgg ccccggcggc ggcgaggatc ctcaccttct
     1501 cggcggcctt ctccgagcgg agggcggcca tgatggggct ctggccctcg gcgccgcgct
     1561 ccgtggcctt ggcccctgcg gcgaggatgg ccttgagcac ctcggggttc tggttgcccg
     1621 agagggcggc gaagagggcg gtctcgcccg cgccgttctt ctcggccagg gaggagcccg
     1681 aggcgaggag gagcctgatc accgcggggt cctcgttgga accggccgcg gccaggaggg
     1741 ggctcgtgcc ctcggcgtcg cgctcgcccg gcttcgcgcc ggccgccatg agggccttga
     1801 gggcctcggg gccggccgtc ctggccgcgc ccacaagggg ggcgagacgc tcgctgtcct
     1861 gggcgctgaa gcgggccccc gcggccacga gggccttgat gcagagcggg tcgcagaggc
     1921 cgagggcggc tcgggcgagg gcagtcttgc cgtccttgtc gcgctcgtcg agcctggcgc
     1981 cgagggacag gaggagtttg accgtctcgg ccttgcccga ggaggccgcg gccatgaggg
     2041 ccgtgccgcc gtcggccacc ttctcctcga tcctcgagcc cgaggacaga aggagcctga
     2101 tcgtcgcggg gttctggccc ccgatggcgg cggccacgag ggcggtcgcg cccagctcgg
     2161 cgccgtagtc gggcctggcc ccggcctcga ggagggcctt gatgacctcg gtgtgggggt
     2221 tggccaggga ggcccagagc agggggctca ccccctcctc gtcgcgggcg tcggccttga
     2281 tccccgaggc caggagcctc cgcacggccg cgagctcgcc ggagcggaag acctcggcct
     2341 cggcctcgga gaagccgggg gccggtttgg ccgcaagggc cctgcccccc gggttgagct
     2401 cgaagtcgag gtcgaggctc gagtactggt agggggtctg cgcccccttg gtcgcggcct
     2461 tcacctcggc agtgacgagc ttcaccgtcc gcgttatgtc cccgggctcg gcgatgaact
     2521 tgaggagggc cgcggcgaag gggctgttgc gcccatcgcc gtcctgggcg ctctggccgg
     2581 cccccgtcga gtagaggacc acggtctcgg gcggggcggc ggccacgacg gccaggcccc
     2641 gcatcgcccc tctgagggcc gagggcaggg gattgtccct gcaggcgtcg aggatgacga
     2701 ggttgacccg gttgtgcgcc tcggcgaggg aggcgaggac cttctgcgcg ctgagggcct
     2761 cgtcctcgag gtcggcggcc gtcctgatct ccgagccgag gggcaggagg tagttgatcc
     2821 cctcgtgctg gatcccgtgg ccggcgaaga agaagaggcc ctcgctaaag gggtccttgg
     2881 cgagggtctc gcggaaggcc acgagggcct ggttcatctg cttgcgcgtg gcgtcggtga
     2941 ggagctgcac ggagaagccg aggcccttga gggcggcggc gaggtcggtg gcgtcgttga
     3001 cggggttcct gagcgcgggc aggcccttgt aggaggcgtt cccgatgacc agggcgtgac
     3061 ggccgccggg ggcctcggcg atggaggctg gagggctgag ggcgaggatg aggagggaca
     3121 gggcaaggga caggacctgg aggcgcaccc tggacaagac tcgcttccca tacatgcggt
     3181 tctcctttgc tgcgccgcga gcctgcggcc gccttcgcgg gattatagca cggggccttg
     3241 gcccggagcc gcgtttctcc ccttttcgga tcggcgatct gccactagac ttggatgctc
     3301 tggcccgccg gcctcgccgc cggggcaagt ccatcgcgaa ccggagcccc atgacccgaa
     3361 gccgatcact ccgcgtcctc ctgctcccgc tcggcctcgg cctgggcctc ctctcctgca
     3421 ccaacccctc gggctggatg gcggcgatct ggccgccgcg catggccagc actctcgcca
     3481 cccccgtcat cggcctgaac agcgggatct acaaggccgc catcagcgtc ccgatcggcg
     3541 gacccgccgg ggccaccctg ctgtataccc tcgacgggac cgacccgacg ccgacctcgc
     3601 ccgtgtatgc ggggccggtg ccgattgccc cgcccgcctc ccaagccggc cagggcctct
     3661 tcgtgctcaa ggtcagggcg agccagagcg gctggaccga ctcggccgtc gcgagcgcga
     3721 ccttcatcgt cgacccctgg atgaagaagt acatcggctc cgggcccgac tcgctctcgg
     3781 acggcatcgc ggccgtcctt gtggccctga acgggccgga tgggatctgc ctcaacgcag
     3841 cgggcgatct ctatgtcgcc gacacgagca acgatgtggt gcgcgtggtg agccacggtt
     3901 ccacactgac ctactttg
//
LOCUS       DASSQV010000358         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2323652, whole genome shotgun sequence.
ACCESSION   DASSQV010000358 DASSQV010000000
VERSION     DASSQV010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323652"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1737
                     /locus_tag="VFL04_06270"
     CDS             271..1737
                     /locus_tag="VFL04_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7839346.1"
                     /translation="MSLESCFAPYRRGIVGIDAEMRLVDGRSRPIVYADWTASGRLYR
                     PIEDFMLAEVGPLVANTHTETTYTGVAMTRAYHRARELIKEAVGAGPEDSLIFSGFGM
                     TSAINKLQRLIGLGRRLRGPRPEGARQRPLVLVTHMEHHSNQITWEECDVDVRIIPRD
                     EATGLPDLAWQERILKENADRPLLIGAFTACSNVTGILTPYRRMAALMHRHGGLCFVD
                     FAASAPYVEIDMHPADPDERLDAITFSPHKFLGGPGSSGVLVLSNALYRNPVPDQPGG
                     GTVKWTTPFGTHRYVDEIEAREDGGTPGFLQAIRAALAMELKLSMGIARIAEREEELK
                     AIVLGELRAESAVMLLDGGNMDRLGIFSLYAPGEHHNLIVRLLNDRFGVQTRGGCSCA
                     GTYGHFLFDIKKSKSLHITDMIDSGDLSEKPGWVRVSLHPTMTDEEARYVGRAVVEVI
                     RNYRVWAADYQFCKESAEFEPKAAGNQAPDLLSRFRPL"
     gene            1937..2185
                     /locus_tag="VFL04_06275"
     CDS             1937..2185
                     /locus_tag="VFL04_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839347.1"
                     /translation="MGAAIIGTGSGVMSKLFDVIAEARWRGELNPGEYRWILDHVCKC
                     WAESRTEEGPPGFAHVEVLLQPKPKADKPHLRRRAGSS"
BASE COUNT          368 a          817 c          694 g          331 t
ORIGIN      
        1 tggccaggcc ccccgcggag gaggactgcg ccgaggcccg ccagggccgc gaggacgagg
       61 acggcggcgg tcaccgcata gggtcttctg ctgccatgct tcatgggctc ggaggatagc
      121 ccgaagcggg gcccggcgcc agggtccagg gcggacggac cgccgtcttc gcgacaccgc
      181 cttggggacg gccttgtgct gggggcccgc gatcaattat aagaagactg cgcccgccca
      241 tgccttgggg gccgctatga ggggaacacc atgagccttg agtcctgttt cgctccctac
      301 cgccgtggca tcgtcggcat cgacgctgag atgcgcctcg tcgacggcag gagccgtccg
      361 atcgtctacg ccgactggac ggcctcgggc cggctctacc gccccatcga ggacttcatg
      421 ctcgccgagg tcggccccct cgtggcgaac acccacaccg agacgaccta caccggtgtg
      481 gcgatgacca gggcctacca ccgcgcccgc gagctcatca aggaggccgt cggcgcaggg
      541 cccgaggact cgctcatctt ctcgggcttc ggcatgacct cggcgatcaa caagctccag
      601 cgcctcatcg gcctcggccg acgcctccgc ggcccgaggc ccgagggcgc gagacagagg
      661 ccacttgtcc tggtgaccca catggagcac cactcgaacc agatcacctg ggaggaatgc
      721 gacgtcgacg tgcggatcat cccgcgcgac gaggcgacgg ggcttcccga cctggcctgg
      781 caggagcgca tcctcaagga gaacgccgac cgccccctcc tgatcggcgc cttcaccgcc
      841 tgctccaacg tcaccggcat cctcacgccc taccgcagga tggccgccct catgcaccgc
      901 cacggagggc tctgcttcgt ggacttcgcg gcctcggcgc cctatgtcga gatcgacatg
      961 caccccgccg atcccgacga gaggctcgac gcgatcacct tctcgcccca caagttcctc
     1021 ggcggccccg gctcctccgg cgtcctcgtg ctctcgaacg ccctctacag gaaccccgtc
     1081 cccgaccagc cgggcggggg caccgtgaag tggacgaccc ccttcggcac ccaccgctac
     1141 gtggacgaga tcgaggcgag ggaggacggg ggcaccccgg gcttcctcca ggccatccgc
     1201 gccgccctcg cgatggagct caagctctcc atgggcatcg cgcggatagc cgagcgggag
     1261 gaggagctca aggccatcgt cctgggcgag ctcagggccg agagcgccgt catgctcctc
     1321 gacgggggca acatggacag gctcggcatc ttctcgctct acgccccggg cgagcaccac
     1381 aacctcatcg tgcgtctgct caacgaccgc ttcggggtcc agaccagggg cggctgcagc
     1441 tgcgcgggca cctacggcca tttcctcttc gacatcaaga agtccaagtc cctccacatc
     1501 accgacatga tcgactcggg cgacctctcc gagaagccgg gctgggtccg cgtctccctc
     1561 cacccgacga tgaccgacga ggaggcccgc tatgtcggca gggccgtcgt cgaggtcatc
     1621 aggaactacc gcgtctgggc cgccgactac cagttctgca aggagtcggc cgagttcgag
     1681 cccaaggcgg cgggcaacca ggcccccgac ctcctctccc gcttccgccc cctgtaggcc
     1741 ctccggcccg gccctcgctc cctgcccgca tccgggcagg gagccactcc cccgagcgcc
     1801 tcagcgcctg atagggatat cacgcggagt gaatttatca ttattttgtg gacggcccga
     1861 gaacccgata attatattgc tatttgcgtc cgcgacacgg cggctcgcgt gggaagatca
     1921 aaacaaggag gctctcatgg gcgcagcgat catcggcacc ggcagcggcg tcatgtccaa
     1981 gctcttcgac gtcatcgcgg aggcccgctg gaggggcgag ctcaatcccg gagagtaccg
     2041 ctggatcctc gaccacgtgt gcaagtgctg ggccgagagc cggaccgagg agggccctcc
     2101 cggtttcgcc cacgtcgagg tcctcctgca gccgaagccc aaggccgaca agccccacct
     2161 gcgccggcgg gccggctcgt cctaagcccc ccagcgctcc cttgttgccc
//
LOCUS       DASSQV010000359         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1078365, whole genome shotgun sequence.
ACCESSION   DASSQV010000359 DASSQV010000000
VERSION     DASSQV010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078365"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFL04_06280"
     CDS             complement(<1..570)
                     /locus_tag="VFL04_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET7839348.1"
                     /translation="MRPPKQLSAAALSPWLRVLLALLLWVAALGAPAAQTVYHVLAKG
                     ETLYSVARSYGVSVEAIAAANGIADPAKARVGQRLLIPAVHKVAKGETLFGIAKDYGV
                     GVDELRSANRLKPETVIKVGDALFIPGAAAASSSSPGPAAASDPVGGTKPNPGSGTSA
                     STQTGPGQTGAGQTGPGLAGTVPAAMKVSP"
     gene            complement(644..>2047)
                     /locus_tag="VFL04_06285"
     CDS             complement(644..>2047)
                     /locus_tag="VFL04_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839349.1"
                     /translation="LAAATSLLDQAEEAQAEAEESSGRASAAEADARLGDALGAYGMA
                     RDSSSSGLSLIEPLGDSTIFSTKGKRTAAELRRSLASSRDGAKAGIDRIGKAEAALAA
                     DDASKAAAAVIDQSLAEADRAQALLDENSAVVGDVASYPPERLVPVRDALAIGRRSLA
                     DAKAALLRADPSLPKDKGYLRDKLEYARRRLDTQDASLLAAWRTVDQEIRDPAVRRAA
                     RAQAWRWAFLHVPKEYLGLRVYTPLGLSTEAEAKRVPFDLQGRAEGAFGSEGGVWARS
                     VIGSEHLEIGSEGAEAQEFRVSQSFDFGFWSRSLFFVGYSWDWYRSVEGQSLSQRPGA
                     VRIGIGGVSRQASGMPRADWTMGLSWEIPYRSEEFLLWNALNAGVDAQFRLGDVAVLE
                     AKIAHRAHLDPGLPAPGLVAVLQWSAALAIRVPPPFLWGVEYFGSLSRPMNASGEAED
                     SSRIGEGSFRFFLGYSL"
BASE COUNT          292 a          694 c          776 g          285 t
ORIGIN      
        1 cggggagacc ttcatggcag cggggacggt ccccgccagg cccggccccg tctggcccgc
       61 gccggtctgg ccaggccctg tctgggtgga cgcggacgtg cccgatcccg ggttcggctt
      121 ggtcccgccg acgggatcgg aagccgccgc cggacccggc gaggaggagg aggccgcggc
      181 cgcgccgggg atgaaaaggg cgtcccctac cttgatgacg gtctcgggct tgagcctgtt
      241 cgccgagcgc agctcatcga ctcccacccc gtagtccttc gcgatcccga acagggtttc
      301 gcccttggcg accttgtgca cggcggggat gaggagcctc tggccgaccc tggccttggc
      361 cggatcggcg atcccgttgg cggcggctat ggcctcgacg ctcaccccgt agctccgcgc
      421 cacggagtag agggtctcgc ccttggccag cacgtggtac acggtctgcg ccgcgggggc
      481 ccccagggcc gcgacccaga ggaggagggc gaggaggacg cggagccagg gggacagcgc
      541 cgccgcggaa agctgcttgg gaggcctcat cagttccgag tatcggcggg aggccgcgaa
      601 ttattgagac gggcctgtgg ccgaaaggcg gaaaagagcg ggtctagagc gaatagccca
      661 ggaagaagcg gaagctgccc tctccgatcc tgctcgagtc ctcggcctcc ccgctggcgt
      721 tcatcggccg ggagaggctc ccgaagtact ccacgcccca gaggaagggc gggggcactc
      781 tgatggcgag ggcagccgac cactggagga cggcgacgag accgggcgcg ggcaggccgg
      841 ggtcgagatg ggcgcgatgg gcgatcttgg cctcgaggac ggcgacgtcg ccgaggcgga
      901 actgggcgtc aactccggcg ttcagggcgt tccagagcag gaactcctcg ctccggtagg
      961 ggatctccca ggagaggccc atggtccagt cggccctggg catgcccgag gcctggcggc
     1021 ttaccccgcc gatcccgatg cgcacggccc ccggccgctg cgagagggac tggccctcga
     1081 ccgacctgta ccagtcccag gaatagccca cgaagaaaag ggacctggac cagaagccga
     1141 agtcgaagct ctgcgacacg cggaactcct gggcctcggc cccttcggag ccgatctcga
     1201 ggtgctcgct tcctatgacc gacctggccc agaccccgcc ctcggagccg aaggccccct
     1261 cggccctgcc ctgaaggtcg aaggggaccc gcttggcctc ggcctcggtg gagagcccga
     1321 ggggggtgta gacgcggagg cccaggtatt ctttgggcac gtggaggaag gcccagcgcc
     1381 aggcctgggc ccttgccgcg cggcggaccg cggggtccct gatctcctgg tccaccgtcc
     1441 tccatgctgc caggagggag gcgtcctggg tgtccaggcg gcggcgggcg tattcgagct
     1501 tgtccctgag gtagcccttg tccttcggca gcgaggggtc ggcgcgcagc agcgcggcct
     1561 tcgcgtcggc gagggagcgc cggccaatgg ccagggcgtc cctgaccggg accaggcgct
     1621 cggggggata ggacgccacg tcccccacca cggccgagtt ctcgtcgagg agggcctggg
     1681 ccctgtcggc ctcggccagg ctctggtcga tcacggcggc cgcggccttc gaggcgtcat
     1741 cggcggcgag ggccgcctct gccttcccta tccggtcgat gccagccttg gcgccgtccc
     1801 gcgaggaggc gagggagcgg cggagctcgg cggccgtgcg tttgcccttc gtggagaaga
     1861 tggtcgagtc gccgaggggc tcgatgaggg agagccccga gctcgaggag tcgcgcgcca
     1921 tcccgtaggc gccgagggcg tcgcccagcc tggcgtcggc ctcggcggcc gaggcccggc
     1981 ccgagctctc ctcggcctcg gcctgggcct cctcggcctg gtcgaggagg gaggtcgcgg
     2041 ccgcgag
//
LOCUS       DASSQV010000360         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1578221, whole genome shotgun sequence.
ACCESSION   DASSQV010000360 DASSQV010000000
VERSION     DASSQV010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578221"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFL04_06290"
     CDS             complement(<1..531)
                     /locus_tag="VFL04_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET7839350.1"
                     /translation="MKVLDEYAAREYLHGRGPRMARARLARDPAEAVAAAESMGWPVV
                     AKASVEGVGHKSDFKGVELDLGGPDELVAAFRRLSASAANAGYGGAFRGVLVEERLRG
                     SEFIIGALRDEAFGPVVMVGSGGVLAELFRDAVFRLAPICAAEAERAIRETRASRFLE
                     GFRGSPPLPIKSLAEAV"
     gene            complement(528..1961)
                     /locus_tag="VFL04_06295"
     CDS             complement(528..1961)
                     /locus_tag="VFL04_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET7839351.1"
                     /translation="MRRALEELFSARGCAVVGASQQPKKAGHQIVANMLAAGYAHGLY
                     PITPSGGEILGCPSYPSVSSIPAEVQLVILSAPSRATASIVEDLKRRMEERGDLIGVV
                     CAAAGFAEVGTPEGKANQELLASFCRDRNLRLVGPNCVGVIDTASRVDTTFIAGIVHA
                     PGGISLASQSGALGAWLLMDWSSTPAGRVGFRRFVTVGNMADVDIIEGIENLGADPGT
                     KVLGLYIEGSPKARDLAFAIGLAASRKPVLALKVGRSSEGARAAKSHTGSMAGADAVY
                     DGVFRQLGILRVRSPRELSDGLRAFDSLPLPRGGRTFILTQAGGPGIICVDECSEAGN
                     IVPAMVSGATKAAIRAVVPEIASVCEPEGHADITAAADADNHVRALEAVLRDDGVDAV
                     ILITVATLVLDLEGMARKMVQTLAALRLEGIDKPVFPVILSGEWVMPCRRVLEEGGLP
                     TWDGPERAVRALSLMIERVERSKRSKE"
     gene            complement(1945..>2464)
                     /locus_tag="VFL04_06300"
     CDS             complement(1945..>2464)
                     /locus_tag="VFL04_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839352.1"
                     /translation="YQRSSSTSKGSWTSTTPVSKEGAGGKSQHRKDFPLIMAMHDLPY
                     VATASPAYIPDLVRKVEKAMEVKAGLAYIHVYNPCLTGWGIKSDDSIAVSRLAVQSRF
                     FPLWEAEGRRLRFTVRVPKPLPVADYVSRIGKFRHLGAERIAELQAVTDEHYRYLEAM
                     DRMGGGDDATGA"
BASE COUNT          349 a          826 c          936 g          353 t
ORIGIN      
        1 gacggcctcg gcgaggctct ttatgggcag gggaggggag ccgcggaagc cctcgaggaa
       61 gcgggaggcc ctggtttcgc gtatcgcgcg ctcggcctcg gcggcgcaga tcggggccag
      121 gcggaacacg gcgtcgcgga agagctcggc gaggacgccc cccgagccca ccatgacgac
      181 ggggccgaag gcctcgtcgc gcagggcgcc gatgatgaac tccgagccgc gcagcctctc
      241 ctcgacgagg acgccgcgga aggccccgcc gtagcccgcg ttcgcggccg aggccgagag
      301 cctcctgaag gcggccacga gctcgtcggg cccgccgagg tcgagctcga cccccttgaa
      361 gtcgctcttg tggccgaccc cctccaccga ggccttggcc acgaccggcc agcccatcga
      421 ctctgcggcg gccacggcct cggccgggtc gcgcgcgagc ctggcgcggg ccatcctggg
      481 ccccctgccg tggaggtatt cgcgggcggc gtattcgtcg aggaccttca ttccttgctc
      541 cgtttcgacc gctcaacgcg ctcgatcatg agtgagaggg cgcggaccgc gcgttcgggc
      601 ccgtcccagg tgggcaggcc gccctcctcg aggacccgcc tgcagggcat gacccattcc
      661 ccggagagga tgacggggaa gaccggcttg tcgatgccct cgagcctgag ggccgcaagg
      721 gtctggacca tcttccgggc catgccctcg aggtcgagga ccaatgtcgc gaccgtgatg
      781 aggatcacgg cgtcgacccc gtcgtccctg agcacggcct cgagggccct gacgtggttg
      841 tcggcgtcgg cggcggccgt gatgtcggcg tggccctcgg gctcgcagac cgaggcgatc
      901 tcggggacga ccgccctgat ggccgccttg gtcgcgccgc tcaccatggc gggcacgatg
      961 ttgccagcct cggagcactc gtcgacgcag atgatgccgg ggcctccggc ctgggtgagg
     1021 atgaaggtgc ggcccccgcg cgggaggggc agggagtcga aggcccgcag cccgtcggag
     1081 agctccctcg ggctgcgcac gcggaggatg ccgagctggc ggaagacgcc gtcgtacacc
     1141 gcgtcggccc cggccatgga gcccgtgtgg gacttggcgg cccgggcgcc ctcgctcgag
     1201 cggcccacct tgagggccag gaccggcttg cgcgaggcgg cgaggccgat ggcgaaggcg
     1261 aggtccctgg ccttgggcga gccctcgatg tagagcccga gcaccttcgt gccgggatcg
     1321 gcgccgaggt tctcgatgcc ctcgatgatg tccacgtcgg ccatgttgcc gacggtcacg
     1381 aagcggcgga agcccacccg gccggccggg gtcgagctcc agtccatgag gagccaggcc
     1441 ccgagggcgc ccgactggga ggcgagggag atgcccccgg gcgcgtgcac gatgccggcg
     1501 atgaaggtgg tgtcgacccg cgaggccgtg tcgatgacgc ccacgcagtt ggggcccacg
     1561 agccgcaggt tccgatcgcg gcagaaggac gcgaggagct cctggttggc cttgccctcg
     1621 ggcgtcccga cctcggcgaa gccggcggcc gcgcagacca ctccgatgag gtcgcccctc
     1681 tcctccatcc ttcgcttgag gtcctcgacg atcgaggcgg tcgcgcgcga gggcgccgag
     1741 aggatcacga gctggacctc ggcggggatc gaggagaccg aggggtagga ggggcagccg
     1801 aggatctcgc cgcccgaggg ggttatcgga tagaggccgt gggcgtagcc cgcggccagc
     1861 atgttggcga ctatctggtg gccggccttc ttaggctgct gggatgcccc gacgacggcg
     1921 cagccccggg ccgagaagag ctcctcaagc gcccgtcgca tcgtcccccc cgcccatcct
     1981 gtccatggcc tcgaggtagc ggtaatgctc gtcggtgacg gcctggagct cggcgatccg
     2041 ctcggcgccg aggtggcgga acttgccgat gcggctcacg tagtcggcca ccggcagggg
     2101 cttggggacg cggaccgtga agcggagcct cctgccctcg gcctcccaga gggggaagaa
     2161 gcggctctgg acggcgaggc gggagacggc gatcgagtcg tcggacttga tgccccagcc
     2221 cgtgaggcag gggttgtaga cgtggatgta ggcgaggccg gccttgacct ccatcgcctt
     2281 ctccaccttc ctcacgaggt cggggatgta ggcgggcgag gccgtggcga cgtagggcag
     2341 gtcgtgcatc gccatgatga gggggaagtc cttgcggtgc tggctcttgc cgccggcccc
     2401 ctccttcgag accggggtgg tgctggtcca ggagcccttc gaggtcgagg aggaccgctg
     2461 gtag
//
LOCUS       DASSQV010000361         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2324656, whole genome shotgun sequence.
ACCESSION   DASSQV010000361 DASSQV010000000
VERSION     DASSQV010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2324656"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFL04_06305"
     CDS             <1..225
                     /locus_tag="VFL04_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839353.1"
                     /translation="ACQAVDLRGGPEGLSPSTAAAYRLVRSAVPSLGADRVMYPDIEA
                     VLALIAEGSLLRAAADHLEGASAAELLDTP"
     gene            236..2257
                     /locus_tag="VFL04_06310"
     CDS             236..2257
                     /locus_tag="VFL04_06310"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET7839354.1"
                     /translation="MATKQDPAHAMTVSLDLDELPPPASFEPGIRRAPKREASLTAEQ
                     EVLALQNALRYVPRKWHEVLAPEFLAELRSRGRIYGYRFRPAGPIVGKPIDSYKGGCV
                     EGRAMQVMIDNNLDFKVALYPYELVTYGETGQVCQNWMQYRLIRKYLELVDEGCTLVV
                     ESGHPLGLFKSHPYAPRAIVTNGLMIGMFDNQKDFNVAAALGVANYGQMTAGGWMYIG
                     PQGIVHGTFNTLLNAGRLKLGIPASGDLRGRLFVSSGLGGMSGAQGKAAVIANAASII
                     AEVDPSRIETRHSQGWVDSVARSAEEAFGLARAAQAEQRPVAVAFQGNIVELLEYADS
                     HNLHIDLLSDQTSCHAAYEGGYCPAGLSFEERTRLLVQDRAAFAKLVDASLRRHFEVI
                     RRLTAKGTYFFDYGNSFLKAVWDAGVREVSKNGTDEKDGFIFPSYVEDILGPELFDYG
                     YGPFRWVCLSGKAEDLRRTDKAAMDCIDPERRGQDRDNWAWIRDAEKNRLVVGTQARI
                     LYQDALGRMRIALRFNEMVRSGEVGPIMLGRDHHDCGGTDSPFRETSNIRDGSNVMAE
                     MATQIFAGNAARGMSLIALHNGGGVGIGKSINGGFGLVLDGSAKVDEVIRMAMPWDVM
                     GGVARRAWARNEHAIEVCEEYNLEHADLGRITLPWPADTESLRRLVAKA"
     gene            complement(2427..3212)
                     /locus_tag="VFL04_06315"
     CDS             complement(2427..3212)
                     /locus_tag="VFL04_06315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7839355.1"
                     /translation="MAKELEGRWALVSGASSGIGAELARELASRGSKLVLVSRRTEKM
                     EALAREIQALGMIEIHVVSADLTERGAPERLLKVLESRGIEVSILANNAGFGAYGPFD
                     SIPAEVEAAMLDLDIKAIVGLTRLFAGPMRKAGWGRILLTASTAAYQPTPLYASYAAA
                     KAFVLSYGLAVRSELEGTGVSVTVLSPGVTRTAFHSVAGHEDNAFKRATMMEARPVAR
                     AAVTALLRCRPEVVPGAVNKLSAFGVRFLSRPAQASMAKRLMS"
BASE COUNT          534 a         1218 c         1036 g          484 t
ORIGIN      
        1 gcctgccagg ccgtcgacct ccgcggcggg cccgagggcc tctcgccctc cacggccgcg
       61 gcctaccgcc tcgtgcgctc ggccgtcccg agcctcgggg ccgaccgcgt catgtacccc
      121 gacatcgagg ctgtcctcgc cctcatcgcc gagggatccc tcctccgcgc cgccgccgac
      181 cacctcgagg gcgcctccgc ggccgagctc ctcgacacgc cctaaggaga aagccatggc
      241 caccaagcag gatcccgcac acgcgatgac cgtaagcctc gacctggacg agctcccgcc
      301 cccggcgagc ttcgagcccg gaatacggcg ggcccccaaa cgcgaggcct cgctgacggc
      361 cgagcaggag gtcctcgccc tccagaacgc cctgcgctac gtgccccgga agtggcacga
      421 ggtcctcgcg cccgagttcc tcgccgagct gcgcagccgg ggccggatct acggctaccg
      481 cttcaggccg gcggggccga tcgtcggcaa gccgatcgac tcctacaagg gcggctgcgt
      541 cgagggccgc gccatgcagg tgatgatcga caacaacctc gacttcaagg tcgcgctcta
      601 cccctacgag ctcgtcacct acggcgagac cggccaggtc tgccagaact ggatgcagta
      661 ccgcctcatc aggaagtacc tcgagcttgt tgacgagggc tgcaccctcg tcgtggagtc
      721 gggccacccc ctcggcctct tcaagagcca cccctacgcg ccgcgcgcca tcgtgaccaa
      781 cgggctcatg atcggcatgt tcgacaacca gaaggacttc aacgtcgccg ccgccctcgg
      841 cgtggccaac tacggccaga tgacggcggg cggctggatg tacatcgggc cccagggcat
      901 cgtccacggc accttcaaca ccctgctcaa cgccgggcgg ctcaagctcg ggatccccgc
      961 ctcgggcgac ctccgcggcc ggctcttcgt gtcctcgggc ctcggcggga tgtcgggggc
     1021 ccagggcaag gccgcggtca tcgccaacgc ggcctcgatc atcgccgagg tcgacccgag
     1081 ccggatcgag acccgccaca gccagggctg ggtggactcg gtagcgcgca gcgccgagga
     1141 ggccttcggc ctcgcccgcg ccgcccaggc cgagcagcgc cccgtcgccg tggccttcca
     1201 gggcaacatc gtcgagctcc tcgagtacgc ggattcgcat aacctgcata tcgacctcct
     1261 ctccgaccag accagctgcc acgccgccta cgagggcggc tactgccccg ccggcctctc
     1321 cttcgaggag aggacccgcc tcctcgtgca ggaccgagcc gccttcgcga agctcgtcga
     1381 cgcctccctg cgccgccact tcgaggtgat ccgcaggctc acggccaagg ggacctactt
     1441 cttcgactac ggcaactcct tcctaaaggc ggtctgggac gctggagtcc gcgaggtctc
     1501 gaagaacggg acggacgaga aggacggctt catcttcccg agctacgtcg aggacatcct
     1561 cggccccgag ctcttcgact acggctacgg gcccttccgc tgggtctgcc tctcgggcaa
     1621 ggccgaggat cttcgtagga ccgacaaagc cgccatggac tgcatcgacc ccgagcgccg
     1681 gggccaggac agggacaact gggcctggat ccgcgacgcc gagaagaaca ggctcgtcgt
     1741 gggcacccag gcccgcatcc tctaccagga cgccctcggc cgcatgagga tagccctgcg
     1801 cttcaacgag atggtccgct caggcgaggt cgggcccatc atgctcggcc gcgaccacca
     1861 cgactgcggc ggcaccgaca gccccttccg cgagacgagc aacatccgcg acggctccaa
     1921 cgtcatggcc gagatggcca cccagatctt cgcgggcaac gccgcccgcg gcatgagcct
     1981 catcgccctg cacaacggcg gtggggtcgg gatcggcaag tcgatcaacg gcggcttcgg
     2041 cctcgtcctc gacgggagcg ccaaggtcga cgaggtcatc cgcatggcga tgccctggga
     2101 cgtgatgggc ggggtggccc gcagggcctg ggcccgcaac gagcacgcca tcgaggtctg
     2161 cgaggagtac aacctcgagc acgcggatct cggccgcata accctgccct ggcccgccga
     2221 caccgaaagc ctgcgccgcc tcgtggcgaa ggcctagggg aactcaccgc ggagagcgct
     2281 gggcatagcc gcggagagcg aggagaggat gtgtgagagg gggactcaat tcgaggggat
     2341 ttgcccttct cccctcctca ccactcctta tcctcgcttc tccctccgcg ccctccgtgt
     2401 cctccgcggt gaaatcctcc ccacccctag ctcatgagcc gtttggccat ggaggcctgg
     2461 gccggccttg acaggaagcg gacgccgaag gccgagagct tattgacggc gccggggacg
     2521 acctcgggcc tgcagcggag cagggcggtg accgccgccc tcgctaccgg cctggcctcc
     2581 atcatcgtcg cgcgcttgaa ggcgttgtcc tcgtggcccg ccacgctgtg gaaggccgtc
     2641 cgggtcaccc cgggcgacag cacggtcacc gatacccccg tgccctcgag ctcgctcctc
     2701 accgcgaggc cgtagctcag gacgaaggcc ttggccgcgg cgtagctcgc gtacaggggc
     2761 gtgggctgat aggccgccgt cgaggcggtg aggagtatcc gcccccagcc cgccttccgc
     2821 atgggcccgg cgaagagccg ggtcaggccc acgatggcct tgatgtcgag gtcgagcatg
     2881 gccgcctcga cctcggcggg gatcgagtcg aaggggccgt aggcgccgaa gcccgcgttg
     2941 ttcgcgagga tcgagacctc gatgccgcgg gactcgagga ccttgagcag ccgctccggc
     3001 gcccccctct cggtgaggtc tgcgctcacc acgtggatct cgatcatccc gagggcctgg
     3061 atttccctgg ccagggcctc catcttctcg gtcctcctcg acacaaggac gagcttcgaa
     3121 cccctcgacg ccaattcgcg ggctagctcg gccccgatcc ccgacgaggc cccgctcacc
     3181 agggcccacc ttccctccag ctcctttgcc atgattcgac ctccaatatg acattgttgc
     3241 ccgatatgtc acaacaaggc ttggcagagt ta
//
LOCUS       DASSQV010000362         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1950418, whole genome shotgun sequence.
ACCESSION   DASSQV010000362 DASSQV010000000
VERSION     DASSQV010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950418"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1832
                     /locus_tag="VFL04_06320"
     CDS             105..1832
                     /locus_tag="VFL04_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HET7839356.1"
                     /translation="MSDAPLPSSSPANTDFIREQVAEDLRSGRYAYVRTRLPPEPNGW
                     MHIGHAKAFIIDYSVAMDFGGTCNLRFDDTNPEKEDMSYVEAITRDSRWLGLDCGDRT
                     FFASDYYEKLYGLAVFLIRKGVAYVDDLSDQEIREYRGTNVPDKNNITATPPGKDSPW
                     RNRSVAENLDLFARMRAGEFPDGSKTLRAKIDMAHPNLVMRDPVMYRIRREPHYRTGN
                     AWCIYPMYDFQHPLSDAIEGVTHSLCSLEYEIHRPLYEWFIREAEVFPSRQIEFSRLN
                     ITHTVLSKRWLLQIVNEGRVSGWDDPRMPTIAGMRRRGYTPEAIRDFIGRIGIAKTDS
                     MVDIQLLEHCVREDLNRRAKRVMAVLKPLRLVIENWPEGRVEELEAVNNPEDPSAGTR
                     KIPFSKVLYIERDDFMETPPPKYFRLFPGNSVRLRYGYIVTCTGFDKDPATGEVTEVR
                     CSYDPATKGGDAPDNRRVKGTIHWVSAEHALPIEARLYGYLFAPERAMEVPAGGSFLD
                     NLSPDSIELADGAFGEPSLREAKGGDRFQFERLGYFCRDLDQSGGRPVFNRTVSLRDS
                     WAKIEKKGG"
     gene            1839..2318
                     /locus_tag="VFL04_06325"
     CDS             1839..2318
                     /locus_tag="VFL04_06325"
                     /inference="COORDINATES: protein motif:HMM:NF018958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HET7839357.1"
                     /translation="MTNNDVLRRLRYALNLPDPKLIELFALAGRSISSQELGALLMRE
                     DEAGYAECDDATMGQFLDGLVSSRRGKREAGPEGGPGLPGPPRARLSNNDILKRLRIA
                     LELVDEDIIAIMLLAGVKLSKSELSALFRKPGHPNFRPCGDQFLRNFLSGLTSKYRV"
BASE COUNT          498 a          937 c          808 g          405 t
ORIGIN      
        1 ggccgagggc cctgggcctc aggccgcgcg gagattgacc taggtccgcg cttggggcca
       61 tagtgtctcc gccgcgcccc tgaagcgcgg tcaaggagca caacatgagc gacgcgcctc
      121 tcccgtcttc cagcccggca aacacagact tcatccgcga acaggtggcc gaggacctcc
      181 gctcgggcag gtatgcctac gtgaggacga ggctgccccc cgagccgaac ggctggatgc
      241 acatcgggca cgccaaggcc ttcatcatcg actactcggt cgccatggat ttcggcggca
      301 cctgcaacct ccgcttcgac gacaccaacc cggagaagga ggacatgtcc tacgtcgagg
      361 ccatcaccag ggactcccgc tggttgggcc tggactgcgg ggaccgcaca ttcttcgcct
      421 cggactacta tgagaagctc tacggcctgg ccgtcttcct gattaggaag ggcgtcgcct
      481 atgtcgacga cctctccgac caggagatcc gcgaataccg cggcacgaac gtcccggaca
      541 agaacaacat cacggcgacc ccgccgggca aggacagccc ctggcggaac cggagcgtcg
      601 ccgagaacct cgacctcttc gcgcgcatga gggcggggga gttccccgac ggatccaaaa
      661 ccctccgagc caagatcgac atggcccacc cgaacctcgt gatgcgcgac cccgtcatgt
      721 accgcatcag gcgggagccc cactaccgca cgggcaacgc ctggtgcatc tatcccatgt
      781 acgacttcca gcacccgctc tccgacgcga tcgagggcgt cacccattcg ctctgctccc
      841 tcgagtacga gatccaccgt ccgctatacg agtggttcat ccgcgaggcc gaggtcttcc
      901 cctcgaggca gatcgagttc tcccgcctca acatcaccca taccgtcctg tcaaagcgct
      961 ggctcctcca gatcgtgaac gagggccggg tctcgggttg ggacgacccg cgcatgccga
     1021 ccatcgcggg catgcgccgc cgcggctaca cgcccgaggc catcagggac ttcatcggcc
     1081 gcatcggcat cgccaagacg gattcgatgg tcgacatcca gctgctcgag cactgcgtcc
     1141 gcgaggacct gaacaggagg gccaagcggg tgatggccgt cctcaagccc ctccgcctcg
     1201 tcatcgagaa ctggcccgag ggaagggtcg aggagctcga ggcggtcaac aaccccgagg
     1261 acccctcggc cgggacgcgg aagatcccct tctccaaggt cctgtacatc gagcgcgacg
     1321 acttcatgga gaccccgccc cccaagtact tccgcctctt ccccgggaac tcggtcaggc
     1381 tgcgctacgg ctacatcgtc acctgcacgg gcttcgacaa ggacccggcc acgggcgagg
     1441 tcaccgaggt gcgctgcagc tatgaccccg ccaccaaggg cggggacgcc ccggacaacc
     1501 gccgcgtcaa gggcaccatc cactgggtct cggccgagca cgccctgccc atcgaggccc
     1561 ggctctacgg ctatctcttc gcgcccgaac gggcgatgga ggtgcccgcc ggcgggagct
     1621 tcctcgacaa cctgagtccc gattcgatcg agctcgctga cggggccttc ggcgagccct
     1681 cgctcaggga ggccaagggc ggcgaccgct tccagttcga gcgcctcggc tacttctgcc
     1741 gcgacctcga ccagagcggg ggcaggcccg tcttcaaccg aacggtgtcc ctcagggaca
     1801 gctgggcgaa gatcgaaaag aagggcggct gaggcgggat gacgaacaac gacgtcctca
     1861 ggcgcctccg ctacgccctc aacctaccgg acccgaagct gatcgagctc ttcgccctcg
     1921 cggggcgctc gatcagctcc caggagctgg gcgccctcct catgagggag gacgaggccg
     1981 gctacgccga gtgcgacgac gccacgatgg gccagttcct cgacggcctc gtgagctcgc
     2041 gcaggggaaa gagggaggcg gggcccgagg gcgggcccgg cctccctggc ccgcccaggg
     2101 cccggctgag caacaacgac atcctcaagc ggctcaggat cgccctcgag ctcgtggacg
     2161 aggacatcat cgccatcatg ctcctcgcgg gcgtaaagct ctccaagtcc gagctgagcg
     2221 ccctattcag gaaacccggc catcccaact tccggccctg cggcgaccag ttcctccgga
     2281 atttcctctc cggcctgacc tcgaagtaca gggtctagag accgacccgg agccgactcc
     2341 agcccctgac cccggccgcc aggccccctc cttccggaaa aaatccgcga ctgtggccct
     2401 tgcttcctcc ttcatgtgac ataatattta ctaacatgga aggcggggga tcccgctgcc
     2461 ctggagagga ggaagccatg gagcagtgcc ctggggtcgt gacatgtccg ttctacctgg
     2521 atcggatggc ggccatgccg actatggccg gcatgatgaa gcggcatttc tgcaacgacg
     2581 attacgtcaa ttgcgcgagg tacatggtca aggacaggct cgggaaggag cgtgtcccca
     2641 tcgacctc
//
LOCUS       DASSQV010000363         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1079171, whole genome shotgun sequence.
ACCESSION   DASSQV010000363 DASSQV010000000
VERSION     DASSQV010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079171"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VFL04_06330"
     CDS             <1..1013
                     /locus_tag="VFL04_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7839358.1"
                     /translation="AGLERDRAMEAFTTGHRRLGKKVLELRGIRKSWDGKEVVPEYSR
                     TFKAGERIGLIGPNGSGKTSFLDLVAGRTAPDSGTVDVGENTHFAYFDQTGSAIDGSM
                     ELLQYVKEHAERIRMKDGTWLEADQLLERFLFPRPMFELKLSQLSGGELRRLQLVRLL
                     AESPNFLLLDEPTNDLDIDTIELLEDFLELFPGCVLAVSHDRAFLDGNVDFLLSFEPG
                     RAISEHVGGYADYRSHREAEEAKRRRAAREEPEARAARDSKQEGAKRRLSYAERREFE
                     AILDLISGLEAERKGLEELFASASPDPAALAAAGARYAGLGREIEDKTARWEELAERA
                     DE"
     gene            1056..1406
                     /locus_tag="VFL04_06335"
     CDS             1056..1406
                     /locus_tag="VFL04_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839359.1"
                     /translation="MKITQKHLTWILMGLFVILVGYALLRGLTGFRLPPKIDSELPNF
                     VMVGALAVFLYGRKLRADETKAAAAAAAAAAAAREAREAGEPGPEDGGKGEEGGQGRG
                     AEPRGPGEDRGRDR"
     gene            1390..2559
                     /locus_tag="VFL04_06340"
     CDS             1390..2559
                     /locus_tag="VFL04_06340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HET7839360.1"
                     /translation="MGETARKTGSFGPLSPEAVSLSVEAVLGEGTDGTLVSYPSYINR
                     VYGLRLEPGRDEVQGRELIAKFYRPGRWTRAAILDEHRFLMDCAEAEIPVASPMRLPD
                     GGTLGEALVEDDEGAEHPFSFALFPKKGGRNFDAEGDEDWLRLGSLIGRFHRVGSSRR
                     ARHRSVCTPVDLSLPFVEELLEAGLVHPEFSAEFSELCRRTLARIAPLFEGVSLGRIH
                     GDCHRGNILDRRDEGLLVIDFDDMMVGPAVQDLWLLLPDHAESCRRQLGLIVEGYEDF
                     MEFDRRELALIEPLRFMRMVYFLAWRSRQREDLWFRESFPDWGGKDFWRLEIGDLRDQ
                     AELVEASLASGGWRTRLGDNEKAEGPAGGRLRRALAAGRDEAMAPQVGAFLFPGR"
     gene            2510..>3092
                     /locus_tag="VFL04_06345"
     CDS             2510..>3092
                     /locus_tag="VFL04_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7839361.1"
                     /translation="MKPWLRRSELFSSLDDDELGAVAADCSASRFTEGTRVFEHGGPG
                     DSLYIVVEGRVSIGGEAAAGPGEGKGGPAVQDGGPVIAELVPGDSFGELELLASRPRN
                     ARATAAADSLLIRFPREGSGFEAIQMKNPALAARILEECLASVAKRIRHANSLLKDNS
                     PWVQELRRQAYADKLTGLNNKAFLEERLEELLSS"
BASE COUNT          500 a         1076 c         1074 g          442 t
ORIGIN      
        1 ccgccggcct cgagcgggat cgggcgatgg aggccttcac gaccgggcac aggaggctcg
       61 gcaagaaggt cctcgagctg cgggggatac ggaagagctg ggacgggaaa gaggtggtgc
      121 ccgagtactc gcggaccttc aaggccgggg agaggatcgg cctcatcggc cccaacggct
      181 cgggcaagac gagcttcctc gacctcgtcg cggggaggac ggcccccgac tcggggacgg
      241 tcgacgtcgg cgagaacacc cacttcgcct acttcgacca gacgggctcg gccatcgacg
      301 gctcgatgga gctcctccag tacgtgaagg agcatgccga gcggatcagg atgaaggacg
      361 ggacctggct cgaggccgac cagctcctcg agcgcttcct cttcccccgg cccatgttcg
      421 agctgaagct ctcccagctc tcgggcgggg agctgcggag gctccagctc gtccgcctcc
      481 tcgccgagtc gcccaacttc ctcctcctcg acgagccgac caacgacctc gacatcgaca
      541 ccatcgagct gctcgaggat ttcctcgagc tcttccccgg ctgcgtcctc gcggtctcgc
      601 acgaccgcgc cttcctcgac gggaacgtcg acttcctcct ctccttcgag ccgggcaggg
      661 caatctccga gcacgtgggc ggctacgccg attaccggag ccaccgcgag gccgaggagg
      721 cgaagcggag gcgggcggcc agggaggagc ccgaggcccg tgcggccagg gactccaagc
      781 aggaaggcgc caagcgcagg ctcagctacg ccgagcgcag ggagttcgag gccatcctcg
      841 acctgatctc gggcctcgaa gccgagcgca agggactcga ggagctcttc gcctccgcct
      901 cccccgaccc cgcggccttg gccgcggccg gcgcgcgcta cgcagggctc ggccgggaga
      961 tagaggacaa gaccgccagg tgggaggagc tcgccgagcg ggccgacgag tgagggggcc
     1021 cctggcccga cagagccacc gaaaaggaga tcgacgtgaa aatcacccag aagcacctta
     1081 cctggatcct catgggcctc ttcgtcatcc tggtcggcta tgccctcctg cgcggcctga
     1141 cgggcttcag gctgccgccc aagatcgact ccgagctgcc caacttcgtc atggtcgggg
     1201 ccctggccgt cttcctctat gggcgcaagc tcagggccga cgagaccaag gccgccgccg
     1261 cagccgcggc cgcggcggcg gcggcgcggg aggcccgcga ggctggggag cctggccccg
     1321 aggatggcgg gaagggggag gagggagggc agggccgggg cgccgagccc agggggcctg
     1381 gagaggaccg tgggcgagac cgctaggaag accggctcct tcggccccct gagccccgag
     1441 gcggtctccc tctccgtcga ggccgtcctc ggcgagggga ccgacgggac cctcgtctcc
     1501 tacccgagct acatcaaccg ggtctatggc ctcaggctcg agccggggcg ggacgaggtc
     1561 cagggcaggg agctcatcgc caagttctac aggcccggcc gctggacccg cgcggccatc
     1621 ctcgatgagc accgcttcct catggactgc gccgaggccg agatacccgt ggcctcaccc
     1681 atgcgtctgc ccgacggagg caccctcggc gaggccctcg tcgaggacga cgagggcgcc
     1741 gagcacccct tcagcttcgc cctcttcccc aagaagggcg gccgcaactt cgacgccgag
     1801 ggcgacgagg actggctcag gctgggctca cttatcgggc gtttccaccg ggtgggcagc
     1861 tcgaggaggg ccaggcaccg ctcggtctgc acccccgtcg acctgagcct ccccttcgtc
     1921 gaggagctgc tcgaggcggg cctcgtccac cccgagttca gcgccgagtt ctcggagctc
     1981 tgcagacgga ccctggcgag gatcgcgccc ctcttcgagg gcgtctcgct cggccggatc
     2041 cacggggact gccacagggg caacatcctc gacaggaggg acgagggcct cctcgtgatc
     2101 gacttcgacg acatgatggt cgggccggcc gtccaggacc tctggctcct cctgcccgac
     2161 cacgccgaat cctgccgccg ccagctcggc ctcatcgtcg agggctacga ggacttcatg
     2221 gagttcgacc gcagggagct cgccctcatc gagcccctgc gcttcatgcg gatggtctat
     2281 ttcctggcct ggcgctcccg gcagagggag gacctctggt tcagggagag cttccccgac
     2341 tggggcggca aggacttctg gaggctcgag atcggcgacc tccgcgacca ggccgagctc
     2401 gtcgaggctt cccttgcttc cgggggatgg cgaactagac tgggggacaa tgaaaaagcc
     2461 gaaggcccgg ccggagggcg gctccggcgc gcccttgccg ccggccgaga tgaagccatg
     2521 gctccgcagg tcggagcttt tctcttccct ggacgatgac gagctcgggg ccgtggccgc
     2581 cgactgctcg gcctcgcgtt tcaccgaggg gacgagggtc ttcgagcacg ggggccccgg
     2641 ggattccctc tacatcgtgg tggagggcag ggtcagcata ggcggggagg ccgcggccgg
     2701 ccccggcgag gggaagggtg gccccgccgt ccaggacggg gggccggtga tagccgagct
     2761 cgtccccggg gattccttcg gcgagctcga gctcctggcc tcaaggccac gcaatgcgcg
     2821 ggcgacggct gccgccgatt ccctcctcat ccgcttcccg agggagggct cgggcttcga
     2881 ggccatacaa atgaagaatc cggccctcgc cgcgaggatc ctcgaggaat gcctggcctc
     2941 ggttgccaag cggatacgcc acgccaactc cctcctcaag gacaactcgc cctgggtcca
     3001 ggagcttagg cgccaggcct acgccgacaa gctcaccggg ctcaacaaca aggccttcct
     3061 cgaggagagg ctcgaggagc tgctttcctc ga
//
LOCUS       DASSQV010000364         3127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_707928, whole genome shotgun sequence.
ACCESSION   DASSQV010000364 DASSQV010000000
VERSION     DASSQV010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707928"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VFL04_06350"
     CDS             complement(<1..901)
                     /locus_tag="VFL04_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7839362.1"
                     /translation="MLASLHTGASLGVWPSVADEAERLGADLFCFPGGRIGLREGHEA
                     SRNAIYDLAARVPLDGLLVWTSSLSGVEQGGAVGAFLDRFRGRPLVSLSSGAPGVPVV
                     TIDYYGGMREAMRHAIKGHGFRRVAFIRGPALHPGAEERYQAYLDSLEAAGIGLDPRL
                     VSPPCPWDSGREALLELIDGRGLVPGRDFLALVASSDLMALWAARTLLARGYRIPEDI
                     AVIGMNNSLESRLSTPPLTTVEGPFAELGAKGFRTLMALVSGEQVEERSTLPATLVVR
                     QSCGCPSSSFVLAATSGGPPPEAS"
     gene            complement(954..1250)
                     /locus_tag="VFL04_06355"
     CDS             complement(954..1250)
                     /locus_tag="VFL04_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839363.1"
                     /translation="MEQESGVFGPMQFGGATLEFPVTFDLRIIYTLAEGSTITDDLER
                     IFASLGVPCALIQGMAKPGAKYGRMGSRITLSSRQQMYSLYSAVGELPYVKTVI"
     gene            1351..2136
                     /locus_tag="VFL04_06360"
     CDS             1351..2136
                     /locus_tag="VFL04_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HET7839364.1"
                     /translation="MPKKRRAPAPVALVSAVVRVFTVLERMSRERSVGLEELSREVGL
                     AKPTVYRFLQTLQELGYIRRDESDKWAMTLKLFNVGSRALDHLDLYSAARPMAEALSE
                     DLGETVHMGVLEGDSAVYVLKIESKYTIRMFSRVGRRIPLYCTAIGKVLLAYMCDDER
                     ERLLADTKLVAFTPKTLTERSALDAELERIRAEGLAHDDEEHEEGIRCIGSPIFDYTG
                     SVVAAMSASWPRFRWDRGDEAQMAAKVKGAAARVSEILGRPAD"
     gene            complement(2172..2426)
                     /locus_tag="VFL04_06365"
     CDS             complement(2172..2426)
                     /locus_tag="VFL04_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7839365.1"
                     /translation="METKICVACGMPMTKPEEYAGGLTTRDYCVHCARPDGSMQSYDE
                     KLRSLAGFIVSTQGLDQGAATAAAKAMMARLPAWSSRAKE"
     gene            complement(2416..3042)
                     /locus_tag="VFL04_06370"
     CDS             complement(2416..3042)
                     /locus_tag="VFL04_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7839366.1"
                     /translation="MSRQEQRGQTRARLIEAASSIMGRDGLLAARTADIAEEAGVAHG
                     TVFTHFPTREDLLCAVIGELGSRLASRLHVLAEGGRELRGILEAHLAALSENEALYAR
                     LVAEGRSLPESARMSVVAIQSAISFHLGIVVERGTREGELRPMPLHLLFNTWIGLIHH
                     YLANGDLFAPDGDSVLLKFGDELLDHFCSLVGRSGDVEPRGKGAVHGD"
BASE COUNT          509 a         1057 c         1076 g          485 t
ORIGIN      
        1 tgctcgcctc cggcggcggc cctcccgacg tggcagcgag gacgaaggag gaggagggac
       61 agccgcagga ctggcgcacg acgagggtgg cgggcagggt gctccgctcc tcgacctgtt
      121 cgcccgagac gagggccatg agggtcctga agcccttggc gccgagctcg gcgaaggggc
      181 cctcgacggt ggtcaggggc ggggtggaca gtctgctctc gaggctgttg ttcatgccga
      241 tcacggcgat gtcctcgggg atcctgtagc cgcgggcgag gagggtcctc gccgcccaaa
      301 gggccatgag gtcactcgag gccacgaggg cgaggaagtc cctgccgggg acgaggcctc
      361 ggccatcgat gagctcgagg agggcctcgc gcccggagtc ccaggggcag ggggggctca
      421 cgagcctggg gtcgaggccg atcccggccg cctcgaggga gtcgaggtag gcctggtatc
      481 gctcctcggc cccggggtgg agggcggggc cgcggatgaa ggcgaccctg cggaagccgt
      541 ggcccttgat ggcatggcgc atcgcctccc tcatgccccc gtaatagtcg atcgtgacca
      601 cggggacccc gggcgccccc gaggagaggc tgaccagggg ccttccccgg aagcggtcca
      661 ggaaggcgcc caccgctccc ccctgctcga ccccgctgag cgatgaggtc cagacgagga
      721 ggccgtccag ggggaccctg gcggcgaggt catagatggc gttgcgggag gcctcgtggc
      781 cctcgcggag gcctatgcgc ccgccgggga agcagaacag gtcggcgccg aggcgctcgg
      841 cttcgtcggc gacgctcggc cacaccccga ggctcgcccc cgtgtgcagg ctcgccagca
      901 cgagcccgat cctgggccgc ctcccgcccg cgtcggacgc gggggggcca ccgtcagatg
      961 acggtcttga cgtaggggag ctcccccacc gccgagtaga ggctgtacat ctgctgccgg
     1021 ctcgagaggg tgatcctcga gcccatgcgg ccgtacttgg ccccgggttt ggccatgccc
     1081 tggatgaggg cgcagggcac accgagtgag gcaaagatcc gctcgaggtc gtcggttatc
     1141 gtcgagccct cggcgagggt gtagatgatg cgcaggtcga aggtgacggg gaactccaag
     1201 gtcgctcccc cgaactgcat gggtccgaaa acgcctgact cctgttccat ggggccaagg
     1261 atggcatgga gcagcggccc ttgtcgagcg gaagcccgcc gtccttgact ctggacctga
     1321 gggggggaca gaatggccta ggagtccgag atgccaaaga agcgccgtgc gcccgcgccc
     1381 gtagccctcg tcagcgccgt ggtgcgcgtc ttcaccgtcc tcgagcggat gtcgcgtgag
     1441 cgctcagtgg ggctcgagga gctctcgcgc gaggtcggcc tggccaagcc gacggtctac
     1501 cgcttcctcc agacccttca ggagctcggc tacatccgcc gcgacgagag cgacaagtgg
     1561 gccatgaccc tcaagctctt caacgtgggc tcccgcgccc tcgaccacct cgacctctac
     1621 tcggcggcca ggcccatggc cgaggccctc tccgaggacc tcggcgagac cgtccacatg
     1681 ggcgtgctcg aaggggactc ggccgtgtac gtcctcaaga tagagtccaa gtacacgatc
     1741 aggatgttct cgcgcgttgg ccgccgcata cccctgtact gcacggccat cggcaaggtc
     1801 ctcctcgcct acatgtgcga tgacgagagg gagaggctgc tcgcggacac caagctcgtc
     1861 gccttcaccc ccaagaccct caccgagcgc tcggccctcg acgccgagct cgagcggatc
     1921 agggccgagg gcctggccca cgacgacgag gagcacgagg agggcatccg ctgcatcggt
     1981 tcgcccatct tcgactacac gggcagcgtc gtcgcggcca tgtcggcctc atggccccgt
     2041 ttcaggtggg accgcggcga cgaggcccag atggccgcca aggtcaaggg cgccgccgcc
     2101 agggtgtccg agatcctcgg cagaccggcc gactaaagcc cccggagggt ctaagggccc
     2161 tcgtccggcc ctcactcttt ggcccgagag ctccacgccg gcagccgagc catcatcgcc
     2221 ttcgcggcgg cagtggccgc cccctggtcg aggccctggg tgctcacgat gaagccggcg
     2281 agactccgca gcttctcatc gtaggactgc atcgagccgt cgggccgcgc gcagtgcacg
     2341 cagtagtccc tggtcgtcag accccccgcg tactcctcgg gctttgtcat cggcattccg
     2401 caggcgacgc agatcttagt ctccatgaac tgctcctttt ccccttggct cgacatcccc
     2461 tgagcggccg accaaggagc agaagtgatc cagaagttca tccccgaact tcagcaatac
     2521 cgaatcgcca tcgggggcaa agaggtcccc gttggcgagg tagtgatgga tgagcccgat
     2581 ccaggtgttg aagaggaggt gcaggggcat cggtctcagc tctccttccc gcgtcccccg
     2641 ctcgacgacg atgcccaggt ggaaggagat ggccgattgg atcgccacga cgctcatccg
     2701 cgccgattcc ggcagggacc tgccctcggc gacgagcctc gcgtacaggg cttcgttctc
     2761 ggagagggca gcgaggtggg cttcaaggat tccccgaagc tcgcggcctc cctcggccag
     2821 gacgtggaga cgcgacgcaa gacgcgaccc aagctccccg atcacggcgc agagcaggtc
     2881 ctccctggtc gggaaatgcg tgaagactgt gccgtgggcc accccggcct cctcggcgat
     2941 gtcggctgtc ctcgccgcga ggaggccatc ccttcccatg atcgaggagg cggcttcgat
     3001 gaggcgggct cgcgtctgcc cccgctgttc ttgccggctc actcgaaggc cgccggaggc
     3061 gctggtttca aggcaggctc cttcacgttg agtctgtact cattgagtcc aaactcaata
     3121 tattcca
//
LOCUS       DASSQV010000365         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1828179, whole genome shotgun sequence.
ACCESSION   DASSQV010000365 DASSQV010000000
VERSION     DASSQV010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828179"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1377
                     /locus_tag="VFL04_06375"
     CDS             151..1377
                     /locus_tag="VFL04_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET7839367.1"
                     /translation="MPSLPLHADTAELFNIAGMLAGIEASGGISRALLAKKLGLSRTT
                     ASTIVSRLLAAGLVAERSSDEGPRLEARADGRGRPGILLDLGTQRWFALGAEYHSGRW
                     AFAITNLKGGIVASATRPVPEDSPEAFLSTLLEGLREAMLLCPGELLPAIGIGAPGLV
                     DCGSGRIIRADDLGWISVPVGETVSAALGLETYVINRNRGSGLAEARFGVGKGVHSLV
                     YIGIGTGISAAFVSDGSLLHGSSYSAGEIGHVVMDAEGPVCRCGKRGCLQVLSSGLAM
                     ARRASALIASGRASSLEPVPPAQLRGEEVCAAAADGDPVATQSLREAASSLGLAVANI
                     ITTFNPDKVILGGPVGQVEGPFLDLVKAETARWAMDYPYQAVRIERGRLGEFTGALGA
                     ACLVLDRKLALALGAR"
     gene            complement(1388..2200)
                     /locus_tag="VFL04_06380"
     CDS             complement(1388..2200)
                     /locus_tag="VFL04_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7839368.1"
                     /translation="MKVGLALGSGGAKGYAHIAYLEVLDQLYIKTSAIAGASMGALIG
                     AFYASGMGAREIRRLAESVSFRSIPHIVDLAGPLDGGIIKGRKIEDWIAENLKAKRFE
                     DLKVPLRIVATDFWRKEQVVFDSGELVPAIRASISIPGVFEPYSLGGRLLVDGGVVNP
                     VPIDLLGDADFVVGIDVSGERAEEAGKDPGRLEVLLGAYGIMGQIIMERRAVDEKVSV
                     YLKPTLRGYNSMEFLKAAEIIESVRDDAEGFRRALIAAKVDRKARDTDRAAG"
     gene            complement(2262..>2943)
                     /locus_tag="VFL04_06385"
     CDS             complement(2262..>2943)
                     /locus_tag="VFL04_06385"
                     /inference="COORDINATES: protein motif:HMM:NF015586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HET7839369.1"
                     /translation="FLSIAGKKMGDIDRLAVTGVGAAFLGRDVLGIEVVSVDEFRAIG
                     LGGLRLSGEKKALVVSVGTGTAFVRAEGRDIRHVGGSGVGGGTIIGLCSRLAGISDFD
                     AIVEATAGGRLENVDLALGDISKVAVGNLPADATASNFGKVSERASGEDLAAGVVNMV
                     FQTVGMIAVMAARAEGVSSAVMVGKSTRLPMARAVLAWISSLYDLRFVVPELAEYATA
                     IGAALALH"
BASE COUNT          444 a         1057 c          995 g          447 t
ORIGIN      
        1 ggggggcctc gagccgggat gggaggaaat agagacgcag ttccccgacg cggacagccc
       61 tgccagcgat catataggat cgacagtagc atggcttggt attttgtcaa gttaatagac
      121 aaaatagtgg agccatgcta agctccgccc atgccaagcc tccccctgca cgcagacacg
      181 gccgagcttt tcaacatcgc gggcatgctg gccggaatcg aggcaagcgg gggcatatcg
      241 agggccctcc tggccaagaa gctgggcctc agcaggacca ctgcctcgac catcgtctcc
      301 cgcctccttg cggcaggcct cgtggccgag cggagctccg acgaggggcc gaggctcgag
      361 gcccgggccg acggccgcgg caggcccggc atcctcctcg acctcggcac ccagagatgg
      421 ttcgccctcg gggccgagta ccattcgggg cgctgggcct tcgcgatcac gaacctgaag
      481 ggggggatcg tcgccagcgc gacaaggccc gtccccgagg acagccccga ggcctttctc
      541 tccaccctgc tcgaggggct cagggaggcc atgctcctct gcccgggcga actccttccc
      601 gccatcggca tcggcgcccc cggcctcgtc gactgcggct cggggaggat catcagggcc
      661 gacgacctcg gctggatctc ggtccccgtc ggcgagaccg tcagcgcagc cctcgggctt
      721 gagacctacg tcatcaaccg caaccgcggc tccggactcg ccgaggcccg cttcggcgtc
      781 ggcaaagggg tgcacagcct cgtctacatc ggcatcggga cgggcatcag cgccgccttc
      841 gtctccgacg gcagcctcct ccacggctcc agctacagcg ccggcgagat cggccacgtc
      901 gtcatggacg ccgagggccc ggtctgccgc tgcgggaagc ggggctgtct ccaggtcctc
      961 tcctctggcc tcgccatggc gaggcgggcc tccgccctca tcgcctcggg gcgggcctcg
     1021 agcctcgagc cggtccctcc agcccagctg cggggcgaag aggtctgcgc agccgccgcc
     1081 gatggcgatc ccgtcgcgac ccaaagcctc agggaggcgg cctcctccct cgggctcgcc
     1141 gtcgccaaca tcatcacgac cttcaacccc gacaaggtga tcctgggcgg ccccgtgggc
     1201 caggtggagg gccccttcct cgacctggtg aaggccgaga cggcgcgttg ggcgatggac
     1261 tatccctacc aggccgtgcg gatcgagcgg ggccgtctgg gggagttcac gggcgcgctc
     1321 ggggctgcct gcctcgtcct cgacaggaag ctggccctcg cgctcggcgc aaggtagggc
     1381 tgcgccttta gcctgcggcc ctgtcggtgt ccctcgcctt gcggtccacc ttcgccgcga
     1441 tgagggcccg cctgaagccc tccgcgtcat cgcggaccga ctcgatgatc tcggccgcct
     1501 tcaggaactc catgctgttg tagcccctga gggtgggctt gaggtatacg gataccttct
     1561 cgtcgacggc gcggcgctcc atgatgatct ggcccatgat gccgtaggcg ccgaggagga
     1621 cctcgagccg gccggggtcc ttgcccgcct cctcggcccg ctccccgctc acgtcgatgc
     1681 ccacgacaaa gtcggcgtcg ccgaggaggt cgatcggcac ggggttgacg accccgccgt
     1741 cgacgaggag cctgcccccg agggaatagg gctcgaatac cccggggatc gagatgctcg
     1801 ccctgatcgc cggcacgagc tcgcccgagt cgaagaccac ctgctccttg cgccagaagt
     1861 cggtggccac gatccgcagc gggaccttga ggtcctcgaa gcgcttggcc ttcaggttct
     1921 cggctatcca gtcctcgatc ttcctgccct tgatgatgcc gccgtccagg gggccggcga
     1981 ggtcgacgat gtgggggatg ctgcggaagg agacggactc ggcgagcctg cggatctcgc
     2041 gggcgcccat gcccgaggcg tagaaggcgc cgatgagggc gcccatgctc gcccccgcga
     2101 tcgccgaggt cttgatgtag agctggtcga gtacctcgag gtaggcgatg tgggcgtagc
     2161 ccttggcccc gccggaaccg agcgccagac cgaccttcac ggcttcctcc tccatggcgg
     2221 ggcccggccc gccgtccccc cgctgggggc cagcctacat tctagtgcaa ggcgagggcg
     2281 gcgccgatcg ctgtggcgta ttcggcgagc tcgggcacca cgaaacgcag gtcgtagagc
     2341 gaggagatcc aggcgaggac ggcgcgggcc atcggcagcc tcgtcgactt gccgaccatg
     2401 acggccgagg agaccccctc ggccctggcg gccatgaccg cgatcatgcc cacggtctgg
     2461 aagaccatgt tgacgacgcc cgccgcgagg tcctcgcccg aggcccgctc cgagaccttg
     2521 ccgaagttcg aggccgtcgc gtcggcgggc aggttgccca cggcgacctt ggatatgtcg
     2581 cccagggcca ggtcgacgtt ctcgagcctg ccccccgccg tcgcctcgac gatcgcgtcg
     2641 aagtccgaga tgcccgccag gcgggagcag agcccgatga tggtcccgcc gccgacgccg
     2701 gagccgccga cgtggcggat gtcccggccc tcggcgcgca cgaaggcggt gcccgtgccc
     2761 acgctcacga cgagggcctt cttctcgccc gagagccgca ggcccccgag gccgatggcg
     2821 cggaactcgt cgacgctcac gacctcgatg ccgaggacgt ccctgccgag gaaggcggcc
     2881 ccgactcccg tcacggcgag gcggtcgatg tcgcccatct tcttgcccgc tatcgagagg
     2941 aac
//
LOCUS       DASSQV010000366         4330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_958209, whole genome shotgun sequence.
ACCESSION   DASSQV010000366 DASSQV010000000
VERSION     DASSQV010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958209"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFL04_06390"
     CDS             complement(<1..703)
                     /locus_tag="VFL04_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET7839370.1"
                     /translation="MSSTSGGRRRGGGGARARGLLAKIGGVLLVASFGLMIPLSLLQY
                     AGIQKATLASFDHELDVNTELIGLALAKPVYEFNTAMLGSLLDSFMVNESIASIEVFD
                     DAGKSLAQKIVMARSFADNMDRQRDLEYQGEKIGKVAISFSSRAKDAIRAEAWSRGRT
                     MLVQNGAIAVLLLLVLTLSLYLTVVRRITQVSSALAEIAEGSGDLTRRLDARSDDELG
                     DLARHFNAFADHLMQD"
     gene            complement(713..1456)
                     /locus_tag="VFL04_06395"
     CDS             complement(713..1456)
                     /locus_tag="VFL04_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7839371.1"
                     /translation="MKRFAFAAAFVLSAFLVVAQDLKTIDVASDEWADCTLKDGSGLY
                     FDVMRLVYGPQGSKLAIKIVPFARSVQMLESGKTDICVGIYQGDVTNGIYPKYPIDFD
                     DLTVMMLKSKVADYKGEASLKDKKVAWIVDYAYDKYLSVPVKLTEVSDTASGIKMLQS
                     GRVDYYIETKSTITPALGTLGISEGAFHLDTVKWVRLYLGFAKNDKGKALQAIWDKRM
                     PVLFKSGELQKAFDKWGFQESYEKLKKDM"
     gene            1738..2667
                     /locus_tag="VFL04_06400"
     CDS             1738..2667
                     /locus_tag="VFL04_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HET7839372.1"
                     /translation="MREIEEVLIVGAGAVGSAVAGMIDRGLPGSVRLLAAPPRSEPLR
                     EQGLILNGERRDFGLVAPGEGGAADLVIVAVKQHQLGRAIEDMSSHVGPETLILSLMN
                     GIESEDLLAAAFGRERVPWAMILGIDAVRLGRETRYSSAGKIHFGDARNPRGAWSARI
                     SRIAAFFDRCGLGYAVPEDMIRSLWFKFMINAGVNQASAVLRAPYAVFQASEPARRLM
                     HAAMREVIALSAALGTGLVEADLGLWETTLSGLRPDGKTSMLQDIEAGRKTEVEAFAG
                     KVVELGSSLGVPVPVNEALLLIIRGLEGRPGTL"
     gene            2839..>4330
                     /locus_tag="VFL04_06405"
     CDS             2839..>4330
                     /locus_tag="VFL04_06405"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET7839373.1"
                     /translation="MIGRILVALMMLAPTAAMADEASLVLPDLRSVSFSGFSGSGLLS
                     AGLAVCALGLLFGLAAFFRLKRLPAHASMKRISELIYATCRTYLATQGKFLSILWFFI
                     AAIMVIYFGPLRGFDALRIIVIIAFSVVGVLGSYGVAWFGIRVNTLANSRSAFASLRG
                     RPYPVYAIPIESGISIGMLLISVELAMMLVILLFVPGDYAGPCFIGFAIGESLGAAAL
                     RIAGGIFTKIADIGSDMMKIVFDLKEDDARNPGVIADCTGDNAGDSIGPTADGFETYG
                     VTGVALITFILLAVHEPTVQVQLLVWIFAMRIVMVLASGAAYLLNGVYGRSRWASSEE
                     MDFEKPLTSLVWIASGISAVMTFLVSALLIPELGDGSLWWKLSTIITCGTLAGALIPE
                     LVKAFTSTNSRHVREIVASAREGGASLVILSGIVAGNFSAYWMGLVILVLMAAAYLIS
                     AVGLAALMVAPAIFAFGLVAFGFLGMGPVIIAVDSYGPVSDNAQSVY"
BASE COUNT          654 a         1470 c         1410 g          796 t
ORIGIN      
        1 tgtcctgcat gaggtggtcg gcgaaggcgt tgaagtggcg ggcgaggtcg ccgagctcgt
       61 catcggagcg ggcgtcgagc cggcgcgtca ggtcgcccga gccttcggct atctcggcaa
      121 gggccgagct cacctgggtg atcctcctca ccacggtgag gtagagggag agggtgagga
      181 cgagaaggag gaggacggcg atggccccgt tctggacgag catggtcctg cccctgctcc
      241 aggcctcggc cctgatcgcg tccttggccc tgctcgagaa cgagatggcc accttgccga
      301 tcttctcgcc ctggtattcg aggtcccgct ggcggtccat gttgtcggcg aaggacctgg
      361 ccatgacgat cttctgggcc aggctcttcc cggcgtcgtc gaagacctcg atcgaggcga
      421 tcgactcgtt caccatgaag gagtcgagga ggctcccgag catcgcggtg ttgaactcgt
      481 agacgggctt ggcgagggcc aggccgatga gctcggtgtt gacgtcgagc tcgtggtcga
      541 atgacgcgag ggtggccttc tggatcccgg cgtactggag gaggctcagg ggtatcatga
      601 ggccgaagga ggcgaccagg aggacgccgc cgatcttggc gaggagtccg cgggccctcg
      661 cccccccgcc gccccgccgg cggcctccgc tcgtgctcga caccgaaggg ccctacatgt
      721 ccttcttgag cttctcgtag ctctcctgga agccccactt gtcgaaggcc ttctggagct
      781 cgcccgattt gaagaggacg ggcatgcgct tgtcccagat cgcctggagg gccttgccct
      841 tgtcgttctt ggcgaagccg aggtagagcc tgacccactt gacggtgtcg aggtggaagg
      901 ccccctcgga gatgccgagg gtcccgaggg cgggggtgat cgtggacttg gtctcgatgt
      961 agtagtcaac gcggccggac tggagcatct tgatcccgct cgccgtgtcc gagacctccg
     1021 taagcttcac cgggaccgag aggtacttgt cgtaggcgta gtcgacgatc caggctacct
     1081 tcttgtcctt gaggctcgcc tcgcccttgt agtcggcgac cttgctcttg agcatcatca
     1141 cggtcaggtc gtcgaagtcg atcgggtact tcggatagat cccgttcgtg acgtcgccct
     1201 ggtagatgcc cacacagatg tcggtcttgc cggactcgag catctggacc gagcgggcga
     1261 aggggacgat cttgatcgcc agcttcgagc cctgtggccc gtagaccagc cgcatcacgt
     1321 cgaagtagag gcccgagccg tccttgagcg tgcagtcggc ccactcgtcg gaggcgacgt
     1381 cgatggtctt gaggtcctgg gccacgacaa ggaaagcgct cagaacaaaa gcggcggcaa
     1441 aagcgaaacg cttcatgatc cctcccaaag gcaaaacggc tggccctagg ccgcggactc
     1501 tcctgttatt agtttatatc ctcataatca taatgcaagt tccggcatat tggaatcggt
     1561 aaaaacgctt cacccggccc cgccgcaggc ggccggggcg tctggcttcc ttgcccagac
     1621 tcgggcagtc cggctacaat cccgcaggtt ccggcccgaa gccttggacg caattgcttt
     1681 cccggcaagg cagcctcccg gtgagggggc ggctggcgga cggatcggag ggatggcatg
     1741 cgggaaatcg aggaggtcct catcgtcggc gccggcgccg tcggctcggc cgtggccggc
     1801 atgatcgaca ggggcctgcc cggctcggtc aggctcctcg ccgctccccc ccgctccgag
     1861 ccgctcaggg aacagggcct catcctcaac ggagagaggc gggacttcgg cctggtcgcc
     1921 cccggagagg ggggcgcggc cgacctcgtg atcgtcgccg tcaagcagca ccagctcgga
     1981 cgggcgatcg aggacatgtc gagccatgtc gggcccgaga ccctcatcct ctccctcatg
     2041 aacggcatcg agagcgagga cctcctcgcc gcggccttcg ggcgggagag ggtgccatgg
     2101 gccatgatcc tcgggatcga cgcggtcagg ctgggccggg agacgaggta ctcctcggcg
     2161 ggcaagatcc acttcggcga cgcccgcaac ccccgcgggg cctggagcgc gcgcatatcg
     2221 cggatcgcgg ccttcttcga ccgctgcggc ctcggatatg ccgtgcccga ggacatgatc
     2281 aggagcctgt ggttcaaatt catgatcaac gccggggtga accaggcctc ggccgtcctc
     2341 cgcgcgccct atgccgtctt ccaggcctcg gagcccgcgc ggcggctcat gcacgccgcg
     2401 atgcgcgagg tcatcgccct ctcggccgcc ctcggcaccg gactcgtcga ggctgacctc
     2461 gggctttggg agacgaccct ctcgggcctc cgccccgacg gcaagacctc gatgctccag
     2521 gacatcgagg cgggcaggaa gaccgaggtc gaggccttcg ccggcaaggt cgtggagctc
     2581 ggctcctcgc tcggcgtccc cgtgcccgtg aacgaggccc ttctccttat catccgcggc
     2641 ctcgagggca ggccggggac tctttaggcg ggcgccgcgc cggcgcaggc agggcgaggc
     2701 cggcgggcga gcgttgtgcg ccggcgccga tttgcttcgg ctcgggaggc ctgagtatat
     2761 ttcggcgtgc atcacgggcg gcccccaagc gcgtgaaggt gcccgcccga tctcgaatcg
     2821 ggaagggtgg aggtccgcat gatcggcaga atcctggtcg ccctgatgat gctcgctccc
     2881 acggccgcga tggccgacga ggcgagcctt gtgctccccg acctgcgcag cgtgtccttt
     2941 tcgggcttct ccgggagcgg cctgctctcg gccggcctcg cggtctgcgc cctgggcctg
     3001 ctcttcgggc tcgccgcctt cttccgcctc aaacgcctgc cagcccacgc ctccatgaaa
     3061 cggatctccg agctcatcta cgcgacctgc aggacctacc tcgccaccca gggcaagttc
     3121 ctctccatcc tctggttctt catcgccgcg atcatggtga tctacttcgg ccccctgcgc
     3181 ggcttcgacg cgctgcggat catcgtcatc atcgccttct cggtggtcgg cgtgctcgga
     3241 agctacggcg tcgcctggtt cgggatcagg gtcaacaccc tcgccaactc ccgttcggcc
     3301 ttcgcaagcc tccgcggcag gccctacccc gtctacgcca tccccatcga atcgggcatc
     3361 tcgatcggca tgctcctcat ctcggtcgag ctcgccatga tgctcgtcat cctccttttc
     3421 gtcccggggg attacgcggg gccctgcttc atcggcttcg ccatcggcga gtccctcggc
     3481 gcggctgccc tcaggatcgc ggggggcatc ttcaccaaga tcgccgacat aggctcggac
     3541 atgatgaaga tcgtcttcga cctcaaggag gacgacgcgc gcaaccccgg ggtcatcgcc
     3601 gactgcaccg gcgacaacgc cggggactcc atcggcccca cggccgacgg cttcgagacc
     3661 tacggggtaa cgggggtggc cctcatcacc ttcatcctcc tcgcggtcca cgagcccacg
     3721 gtccaggtcc agctcctcgt ctggatcttc gcgatgcgca tcgtcatggt cctggcgagc
     3781 ggggcggcct atctcctgaa cggggtctac ggcaggtcgc gctgggccag ctcggaggag
     3841 atggatttcg agaagcccct gacctccctc gtatggatcg cctcgggcat ctcggcggtg
     3901 atgaccttcc tcgtctcggc cctcctcatc ccggagctcg gcgacggcag cctctggtgg
     3961 aagctctcga ccatcatcac ctgcggcacc ctggccggcg ccctcatccc cgagctcgtc
     4021 aaggccttca cctcgaccaa ctcccgccac gtgcgggaga tcgtcgcctc ggcacgcgag
     4081 ggcggggcct ccctggtcat cctctcgggc atcgtcgcgg gcaacttcag cgcctactgg
     4141 atgggcctcg tgatcctggt cctcatggca gctgcctacc tcatctcggc cgtcggcctg
     4201 gcagccctca tggtagcccc cgcgatcttt gccttcggcc tcgtggcctt cggcttcctc
     4261 ggcatggggc cggtgatcat agccgtggac tcctacggac cggtgagcga caacgcccag
     4321 tcggtctatg
//
LOCUS       DASSQV010000367         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_211971, whole genome shotgun sequence.
ACCESSION   DASSQV010000367 DASSQV010000000
VERSION     DASSQV010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211971"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFL04_06410"
     CDS             complement(<1..399)
                     /locus_tag="VFL04_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7839374.1"
                     /translation="MTISARTLKSDALLLTTAIIWGFAFVAQRSGMEFIGPFAYNGIR
                     FAFGAASLLPLLWLGRRSAAATPPATAGRPSTASKLVGSAIAGTVLFLGSSLQQMGLV
                     STTAGNAGFITSLYVVLVPMMGALLGKPTGP"
     gene            484..2568
                     /locus_tag="VFL04_06415"
     CDS             484..2568
                     /locus_tag="VFL04_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7839375.1"
                     /translation="MPSAILHSLAAIVPFRYLREAELRSLLEDSEQRSYEAGDVLIDQ
                     GDLSTSEVYVLISGSVESVDMTRTPPFRMNVVESGSFFGERACLLDNPRTFRIRALEA
                     VTCLVITGERFLRLLASSRSFAQGLGTKLREGYGIFEAFDRFNSEIAQAVADGHVEVR
                     RIVELYKQLEPALHPLASDEARIDWAALTYAVRRLPQNLTRSFVFLLTDNLPTVYASP
                     ELLFPFVPTESRHRFVYEMMSGKDMVLVRDGLSDLVDFITCLCVFAVEAKKIRYRLNH
                     PDLILALEGAKGRDGSRAEALLERLPFDEEERAELRRLWPTETATRIREIVFHRQAFS
                     LDIRKQVNNYNGRLSEHWASQVGAAAGALLGVRPSDFPEELEVHIVSSNTHSVSNCLN
                     PFFARHAEEILGWAGAEGLRGETWTEPFDEVYQLARRWLDAFPEHKAELEETERVCRI
                     VRLPETVTTGIQVQLIDAAAVCGKTADPALSFGECRSPAIIVNIDYAFGEQAEEIMRS
                     LLLLFGSNVRSINVLGKAGALVGSRGDVILPTAFIEQSNDAFQPLPGEDASAVSRLKA
                     VLPGRGIHTGPLLTVGGTLLQNRAMLQFYRRIWGCVGLEMEGIWYLRAVFEAEQLGVL
                     RPGAKRRFLYYVSDVPLAAGQRLSERMAPTEGIPPLYAITREVLGSLFRPSPSPAISG
                     PALRALSESPIL"
     gene            2600..>2880
                     /locus_tag="VFL04_06420"
     CDS             2600..>2880
                     /locus_tag="VFL04_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7839376.1"
                     /translation="MLDRIGRAVRLGLEEFLYGLGFLILVLKESALLFRRRQVGFRVL
                     VMQILFTGFEALSISAVTAVAIGAAINLIGSSLLAEFGQSRLMYTLLIVV"
BASE COUNT          463 a         1036 c          913 g          468 t
ORIGIN      
        1 cgggccggtc ggcttgccga ggagggcccc catcatgggc acgagcacca catagaggct
       61 cgtgatgaag cccgcgttgc ccgccgtggt cgagacgagg cccatctgct ggaggctgga
      121 gccgaggaag agcaccgtgc ccgcgatggc cgaaccaaca agcttcgagg ccgtgctcgg
      181 tcttcccgcg gtggccggtg gcgtggcggc ggcgctccgg cggccgagcc agagcagggg
      241 gagaagggac gccgccccga aggcgaagcg gatgccgttg taggcgaagg gtccgatgaa
      301 ctccatcccg ctgcgctggg ccacgaaggc gaagccccag atgatggccg tcgtgagcag
      361 gagagcgtcg gacttcaggg tcctggctga tatcgtcacg ggagatcccc cttggtgcag
      421 gccccgagcc tatccgaggg aggctttctt gcgctactcc caaggggagg ctaagctttg
      481 gcaatgccca gcgcgatcct gcattccctc gccgccatcg tccccttccg ctacctcagg
      541 gaggccgagc tccgttccct cctcgaagac tccgagcagc ggagctacga ggccggcgac
      601 gtcctcatcg accagggcga cctctccacg agcgaggtct atgtcctcat ctccggctcg
      661 gtcgagtcgg tcgacatgac gcggaccccg cctttcagga tgaacgtcgt cgagtcgggc
      721 tccttcttcg gcgagcgggc ctgcctcctc gacaacccgc gcaccttccg catccgcgcc
      781 ctcgaggccg tgacctgcct cgtcatcacc ggcgagcgct ttctccgcct cctcgcctcg
      841 tcccggtcct tcgcccaggg cctggggacc aagctgcgcg agggctacgg catcttcgag
      901 gccttcgacc gcttcaactc cgagatcgcc caggcggtcg ccgacggcca cgtcgaggtg
      961 cgcaggatcg tcgagctgta caagcagctg gagcccgccc tccatcccct ggcctcagac
     1021 gaggcgagga tcgactgggc cgccctcacc tacgcggtga ggaggctgcc ccagaacctc
     1081 acccgctcct tcgtcttcct cctcaccgac aacctcccaa ccgtctacgc cagccccgag
     1141 ctcctcttcc ccttcgtgcc caccgagtcg cggcaccgct ttgtctacga gatgatgagc
     1201 ggcaaggaca tggtcctcgt ccgagacggg ctctcggacc tcgtcgactt catcacctgc
     1261 ctctgcgtct tcgcggtcga ggcgaagaag atccgctacc ggctcaacca ccccgacctc
     1321 atcctcgccc tcgagggagc gaaggggagg gacggatcga gggccgaggc cctcctcgag
     1381 aggctgccct tcgacgagga ggagagggcc gagctcaggc ggctctggcc caccgagacg
     1441 gcgacgagga tcagggagat cgtcttccac cgccaggcct tcagcctcga catcaggaaa
     1501 caggtcaaca actacaacgg caggctctcg gagcactggg cgagccaggt cggcgccgcg
     1561 gcgggcgccc tcctcggggt gaggcccagc gatttccccg aggagctcga ggtccacatc
     1621 gtctcctcca acacccactc ggtctcgaac tgtctgaacc ccttcttcgc gagacacgcc
     1681 gaggagatcc tcggctgggc cggggccgag ggcctgcgcg gcgagacctg gaccgagccc
     1741 ttcgacgagg tctaccagct cgcccggcgc tggctcgacg ccttcccgga gcacaaggcc
     1801 gagctggagg agaccgagcg cgtctgcagg atcgtcaggc ttcccgagac ggtcacgacc
     1861 gggatccagg tccagctcat cgacgcggcg gccgtctgcg gcaagacggc cgaccctgcc
     1921 ctcagcttcg gcgagtgcag aagccccgcc atcatcgtca acatcgatta cgccttcggc
     1981 gagcaggccg aggagatcat gcgcagcctc ctcctcctct tcggcagcaa cgtgcggagc
     2041 atcaacgtcc tgggcaaggc aggggccctg gtcggcagcc gcggcgacgt gatccttccc
     2101 acggccttca tcgagcagtc caacgacgca ttccagcccc tgcctggcga ggacgcgagc
     2161 gctgtctcgc ggctcaaggc cgtcctgccg ggaaggggca tccacacggg ccccctgctc
     2221 acggtcgggg ggaccctcct ccagaaccgg gcgatgctcc agttctaccg gcggatctgg
     2281 ggctgcgtcg gactcgagat ggagggcatc tggtacctcc gcgccgtctt cgaggccgag
     2341 cagctcggcg tcctcaggcc cggggccaag cgccgcttcc tctactacgt gagcgacgtc
     2401 cccctggccg ccggccagag gctttcggag cgcatggcac cgaccgaagg cataccgccc
     2461 ctgtacgcga taacccggga ggtcctcgga tcgctttttc ggccctcgcc ctccccagcc
     2521 atctcgggac cggccttgag ggccctgagc gagagtccga tactgtaggg tagcgcgcag
     2581 accgcgcggc gggagatcca tgctcgatcg aatcggccgc gccgtgaggc tcggcctgga
     2641 agagttcctc tacggcctcg gcttcctcat cctcgtgctc aaggagtctg ccctcctctt
     2701 ccggaggagg caggtgggct tccgcgtcct tgtcatgcag atcctcttca ccggcttcga
     2761 ggccctgagc atctcggccg tgacggcggt cgcgataggc gcggcgatca acctcatcgg
     2821 ctcatccctg ctcgccgaat tcgggcagag ccgcctcatg tacaccctcc tcatcgtggt
//
LOCUS       DASSQV010000368         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1080251, whole genome shotgun sequence.
ACCESSION   DASSQV010000368 DASSQV010000000
VERSION     DASSQV010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080251"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFL04_06425"
     CDS             complement(<1..812)
                     /locus_tag="VFL04_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839377.1"
                     /translation="MDRRQYDGERRGAYDRRWPRAARLERSARENLLVEAVFFYLYDV
                     GRAVDLSEVSELIPAHPDIGVAKSRDTPTSLTLPKPLILRLGDADCPDLQCEETVSSH
                     AKIYEEGAVTVIIRIKARMPLGELPHLKERVVLSQGKRYTIESFAEAGFRRLFEAVKP
                     AVADPHEPGEMDRESYLAYCVLECEGDPARFLSRNREFCAALLMAESQDSALHESQIE
                     ATLGKPFAYREGELAVFDLDRCLIIDPTGDYEDVLLIAEHANYQLLELRVLDG"
     gene            complement(911..1987)
                     /locus_tag="VFL04_06430"
     CDS             complement(911..1987)
                     /locus_tag="VFL04_06430"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET7839378.1"
                     /translation="MKLNFRLPKLPKLPKVSDLRLPTLPLDLRKIETFDKDHYKVIAY
                     GLGAIIVLMVVAGLTAFLLSLRGAEQTMVPDIRGMELSQALVKLQEKELYPRVALRFT
                     DNPADKGNIVDQSPAPGAIVKAGRRIQITVSRGAVADKVENFVGQDINEVKTHLLTVF
                     AGGKAIITVKDPPVYIFDKSSAGTILEQKPLPDTQISGATSLEFVVSRGPEKAQILVP
                     DLMGLSISDVCSQVQKTDLVVKFTMRAPGKSEKPGSVVAETPTPGSLIAANARVAVTI
                     AAPAPSKGLASGIYSRDLPEYPYPLKVSLEALSANGDRTPVITVNHPGGSFTAPYLLP
                     EDSVLVLTVLDREVPPRVDVKASQ"
     gene            complement(1984..>2695)
                     /locus_tag="VFL04_06435"
     CDS             complement(1984..>2695)
                     /locus_tag="VFL04_06435"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET7839379.1"
                     /translation="FLDLFPKGGVNVHPSLLPRYRGPAPIPSAILARDTETGITVQRL
                     ALEMDSGDILGVLRIPLNGMETAEDLAEVAAIMGANLLSQVLEEIEQGTSTSVPQEGE
                     VSYCTMLKKEDGLVDWRQSVLDIDAMVRAFYPWPGAFTYLKGQRLNILESFPFPHVTF
                     AAGPTSLDFEDAVPGTVLGLDKSRGLMVQTVDGLLALRRLQIQHKKALPYREFANGVR
                     DLAGAVLGRPSPEESDRP"
BASE COUNT          411 a          793 c         1020 g          471 t
ORIGIN      
        1 ccgtcgagga cccgcagctc gaggagctgg taattcgcat gctcggcgat gaggaggacg
       61 tcctcgtagt cgccggtcgg gtcgatgatg aggcatcgat ccaggtcgaa gaccgcgagc
      121 tcgccctcgc ggtacgcgaa aggcttgccc agggtggcct cgatctggct ctcgtggagg
      181 gcggagtcct gcgactcggc catgaggagg gcggcgcaga actcgcggtt gcgggagagg
      241 aagcgggcgg ggtcgccctc gcactcgagg acgcaatacg ccagatagga ctcccggtcc
      301 atctcccccg gctcgtgggg atcggccacc gcgggcttca cggcctcgaa gaggcggcgg
      361 aagccggcct cggcgaagct ctcgatcgta taacgttttc cctgggagag gacgacccgt
      421 tccttcaggt gcggcagctc gccgaggggc atccgcgcct tgatccgtat gatgacggtg
      481 acggcccctt cctcgtagat cttggcgtgg gaggacacgg tctcctcgca ctggaggtcg
      541 gggcagtcgg cgtcgcccag gcgcaggatg aggggcttgg gcagggtgag cgaggtcggg
      601 gtatcgcggc tcttcgcgac gccgatgtcg gggtgggcgg ggatgagctc ggagacctcg
      661 gagaggtcga cggcgcggcc cacgtcgtag aggtagaaaa agaccgcctc gacgaggagg
      721 ttttcgcggg ccgagcgctc gagcctcgcc gcccgcggcc aacggcgatc gtaggccccg
      781 cggcgttcgc cgtcgtactg acgccgatcc acgtaggggc ggctgggttg cccgccgaga
      841 gtggggctcc ggtccgcgcc gcgagggccg cccaaccggg acggcatgcg ttcgctcgag
      901 ccccgcctcg tcattggctc gccttcacgt cgacccgggg cgggacctcg cggtcgagca
      961 ccgtgagcac gaggacggag tcctcgggga ggaggtaggg cgcggtgaag ctgccgccgg
     1021 ggtggttgac cgtgatgacg ggcgtgcgat ctccgttggc gctgagcgcc tcgaggctga
     1081 ccttgagggg ataggggtac tcgggcaggt cacggctgta gataccggag gcgaggcctt
     1141 tggacggcgc cggggccgcg atggtcacgg cgacccgcgc gttggccgcg atgagcgaac
     1201 ccggggtcgg cgtctcggcg acgacggagc cgggcttctc gctcttgccg ggggcgcgca
     1261 tcgtgaactt cacgacgaga tccgtcttct gtacctggct gcagacgtcg gagatggaaa
     1321 gccccatgag gtcgggcacg aggatctggg ccttttcggg gccgcggctc acgacgaact
     1381 cgaggctcgt cgcaccggag atctgggtgt cgggcagggg tttctgctcg aggatcgtgc
     1441 ccgcggagga tttgtcgaag atgtagaccg gcggatcctt gaccgtgatg atggccttgc
     1501 cgccggcgaa gacggtgagg aggtgggtct tcacctcgtt gatgtcctgg ccgacgaagt
     1561 tctccacctt gtccgcgacc gcgccgcggc tgaccgtgat ctggatgcgg cggcccgcct
     1621 tgacgatcgc gccgggggcg ggactctggt ccacgatgtt gcccttgtcc gcggggttgt
     1681 cggtgaaacg cagggccacg cgcggataga gctctttctc ctgcagcttt acgagggcct
     1741 gcgaaagctc catgccgcgg atgtcgggca ccatggtctg ctcggccccg cggagcgaga
     1801 ggaggaaggc ggtcagaccg gccacgacca tgaggacgat gatggccccg agcccgtagg
     1861 cgatgacctt gtagtggtcc ttgtcgaagg tttcgatctt gcggaggtcg agggggagcg
     1921 tgggcagtct caggtccgag accttgggca gtttgggaag cttggggagt ctgaagttga
     1981 gcttcatggg cggtccgatt cctccgggga cggtcgcccc aggactgccc ccgcgaggtc
     2041 ccgtaccccg ttggcgaatt ccctgtacgg gagagccttt ttatgctgga tctgcaggcg
     2101 cctcagggcc aagaggccgt cgaccgtttg taccatcagg ccgcggctct tgtctaggcc
     2161 gagcacggtt ccgggcaccg cgtcctcgaa gtcgaggctc gtcgggcccg ccgcgaaggt
     2221 gacgtggggg aaggggaagg actcgaggat gttgagcctc tgtcctttca ggtaggtgaa
     2281 ggcgccgggc cacgggtaga aggcgcgcac catggcgtcg atgtcgagga cgctctggcg
     2341 ccagtccacg agcccgtctt ccttcttgag catcgtgcaa taggagacct cgccttcctg
     2401 gggcacggag gtggaggtgc cctgctcgat ctcctcgagc acttgggaga ggaggttggc
     2461 acccatgatt gcggcgacct cggcgaggtc ttccgcggtc tccatcccgt tcaaggggat
     2521 gcggaggacg cccaggatgt cgcccgagtc catctcgagg gcgagccgct gtacggtgat
     2581 gcccgtctcc gtgtcgcggg cgaggatggc cgaggggatg ggcgcgggcc ctcgatatct
     2641 gggcaggagg ctcggatgga cgttgacgcc gcccttggga aaaagatcga ggaat
//
LOCUS       DASSQV010000369         3473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2077216, whole genome shotgun sequence.
ACCESSION   DASSQV010000369 DASSQV010000000
VERSION     DASSQV010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077216"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="VFL04_06440"
     CDS             <1..1690
                     /locus_tag="VFL04_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7839380.1"
                     /translation="DLAILGLGAADVPRGCDATEQEIRYILDFSGCRIAILENEKQLR
                     KVIAQKPGIPGLKTLILLDQPEAAALEAAKAAGLSLLGFSEVLARGAPRLARSPREYD
                     EEVAKGSREELATIIFTSGTTGEPKGVMLSHGNFLHQTDQIPKILMIKPGDTWLSVLP
                     VWHSFERILQYVVLASSSSLAYSKPIGAVMLADMQAVRPQWMASVPRIWESVKDGVYR
                     NIKAQGGAKQALFSFFVASGELYAYFRNHLLGRMPEFKPRSRLLELAASIVPFLLLSP
                     IQALGELLVFGKIKAKLGGRFVAAVSGGGALPPAVDRFFDAIGILVLEGYGLTETAPD
                     LAVRHQRRPVVGTVGPALYGTELKVVDEQGKTLPPGHKGLILARGPQVMSGYYNRPEL
                     TAKVLRPDGWLDTGDLGMMTRKGELRITGRAKDTIVLLGGENVEPTPIEHKLAESEYI
                     TQAVVLGQDKKYLAALIVPDQAAVTAWAQENNVPIVDYETLLQQPEVVELIDYEVNEL
                     VSAKNGFKSFERIFRFALLPAVFETGRELSAKQEIKRHAILEIYARQVKKLFEA"
     gene            complement(1691..2569)
                     /locus_tag="VFL04_06445"
     CDS             complement(1691..2569)
                     /locus_tag="VFL04_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET7839381.1"
                     /translation="MSSSEGSGEPGYYNEALEVGQAERLVLRFPRGELLVLPGEGDKI
                     VLELQLRGPGERLGLWRPSVRRREGIIVVADESLDGVMVFEARVSVPASFRDVEAHTA
                     SGSLEFRGIAADLLAATDSGDIRVVGGAAVELSSAAGRVDVEGAAGVNARAGSGEVRC
                     RDITGPVVAETESGDILVEAATGNVVVLSASGDISIQRPGGRLRVATSSGDVELEIVG
                     RFAGGEVSTSTGDVSLALAGSELELRAETLSGELDAPGGEVSVTSGPRRCALRLGAGG
                     RRLHVRSVSGDIEIEV"
     gene            2796..3461
                     /locus_tag="VFL04_06450"
     CDS             2796..3461
                     /locus_tag="VFL04_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839382.1"
                     /translation="MDQNELETLLHDIEELRRAVRRNNPFLRQVLSSRLYYLLTLVIG
                     LVVLAGSLAAQVLVGTWGSFGAMPEAWRAAFYSAAAIVFLVGGGMKWVLFNRRARLIR
                     REANYLTVIKAFYAGSWFHINVPSYLCALAGIGFAIALGHPWYALPVSFVFIALLFNN
                     LGSGIQRPEYLVSGWYALVTALASFLFVERAPFVWLAVVLGGFCLVFAVTGLLGRDGG
                     GER"
BASE COUNT          550 a         1252 c         1124 g          547 t
ORIGIN      
        1 cgacctcgcg atcctcgggc tcggagccgc cgacgtgccc cggggctgcg acgcgaccga
       61 gcaggagatc cgctacatcc tcgacttctc cggctgcagg atcgccatcc tcgagaacga
      121 gaaacagctg cgcaaggtga tcgcccagaa gccgggcatc cccgggctca agaccctcat
      181 cctcctcgac cagcccgagg ccgcggccct cgaggcggcg aaggcggcgg ggctcagcct
      241 cctcggcttc tccgaggtgc tggccagggg ggctccccgg ctcgcgcgca gcccgaggga
      301 gtacgacgag gaggtcgcga agggctcgag ggaggagctc gcgacgatca tcttcacctc
      361 gggcaccacg ggcgagccca agggcgtcat gctgagccac ggcaacttcc tccaccagac
      421 cgaccagatc cccaagatcc tcatgatcaa gcccggggac acctggctct cggtcctccc
      481 cgtctggcac agcttcgagc gcatcctcca gtacgtggtc ctggcctcct cctcgtccct
      541 ggcctactcc aagccgatcg gcgccgtgat gctggccgac atgcaggcag tgaggcccca
      601 gtggatggcc tcggtgccga ggatctggga gtcggtgaag gacggggtat ataggaacat
      661 caaggcccag ggcggggcga aacaggccct cttctccttc ttcgtggcct cgggcgagct
      721 ctacgcctat ttccgcaacc acttgctcgg gcggatgccc gagttcaagc cccgctcgag
      781 gctcctcgag ctggcggcct cgatcgtccc cttcctcctg ctctcgccga tccaggccct
      841 cggggagctc ctggtcttcg ggaagatcaa ggccaaactc ggcgggcgct tcgtcgccgc
      901 agtttcggga gggggggccc tgcccccggc ggtcgaccgc ttcttcgacg caataggcat
      961 cctcgtgctc gagggctacg gcctcaccga gaccgctccc gacctcgcgg tgcggcacca
     1021 gcgcaggccc gtcgtgggca ccgtgggtcc ggccctttac ggcaccgagc tcaaggtcgt
     1081 cgacgagcag ggcaagaccc tgccgccagg gcacaagggc ctcatcctgg cccgggggcc
     1141 ccaggtcatg tcgggctact acaaccgccc cgagctcacg gccaaggtcc tcagacccga
     1201 cggctggctc gatacgggtg acctcgggat gatgacgagg aagggcgagc tgcggatcac
     1261 gggcagggcc aaggacacca tcgtcctcct cggcggcgag aacgtcgagc cgacccccat
     1321 cgagcacaag ctcgccgaga gcgagtacat cacccaggcc gtggtcctcg gccaggacaa
     1381 gaagtacctc gcggccctca tcgtgcccga ccaggccgcg gtgaccgcct gggcccagga
     1441 gaacaacgtc cccatcgtcg actacgagac cctcctccag cagcccgagg tggtcgagct
     1501 catcgactac gaggtcaacg agctcgtgag cgcgaagaac ggcttcaaga gcttcgagag
     1561 gatcttccgc ttcgccctcc tgccggccgt cttcgagacg ggcagggagc tctcggccaa
     1621 acaggagatc aagcgccacg cgatcctgga gatctacgcg aggcaggtga agaagctctt
     1681 cgaggcctga ctagacctcg atctcgatgt cgccggagac cgagcggacg tgaagccggc
     1741 gcccccccgc gccgagcctc agggcgcagc ggcgggggcc cgaggtgacg gacacctcgc
     1801 cccccggggc gtccagctcg ccggagaggg tctcggccct gagctcgagc tcggagcccg
     1861 caagggcgag gctgacgtcg ccggtcgagg tgctcacctc gcccccggcg aaacggccca
     1921 cgatctcgag ctcgacgtcg cccgagctgg tggccacgcg gagcctgccg ccggggcgct
     1981 ggatggagat gtcgcccgag gcgctgagca ccaccacgtt ccccgtggcc gcctcgacga
     2041 ggatgtcgcc cgactcggtc tcggccacga cggggccggt gatgtccctg cagcgcacct
     2101 cgcccgatcc ggccctggcg tttaccccgg cggccccctc gacatcgacc cttcccgccg
     2161 ccgatgagag ctcgacggcg gccccgccga cgacgcggat gtcgcccgag tcggtcgccg
     2221 cgaggaggtc ggcggcgatg ccgcggaact cgagggagcc cgaggcggtg tgggcctcga
     2281 cgtcacggaa cgaggccggc accgagaccc tggcctcgaa gaccatcacc ccgtccaggc
     2341 tctcgtcggc gacgacgatg atgccctcgc gccgcctgac cgatggcctc cagagcccaa
     2401 gccgctcgcc cggcccccgc agctggagct caaggacgat cttgtcgccc tcccccggga
     2461 ggacgaggag ctcacccctc ggaaagcgga ggacgagcct ctcggcctgg ccgacttcga
     2521 gggcttcatt atagtaaccg ggctcgcccg agccttcact gctgctcatc tctgcctccc
     2581 acaacgctgc ctgctcgacg gagcatggaa ttggcggagt atactatcac attttctgct
     2641 catgggcaac gcgatagccg cggcgcccgc cggtccgtgg ccgggcaccg ggggcctgat
     2701 cccgcccggg cacgggacgg gggcttgacc atctcccaga aagccgtata cttcaaacta
     2761 gtttgtgtgg taaacttgtt tgtggaggtg acggcatgga ccagaacgag ctcgagaccc
     2821 ttctccacga catcgaggag ctgaggcggg cggtcagacg caacaacccc ttcctgcggc
     2881 aggtcctctc ctcgcgcctg tactatctgc tgaccctggt catcggcctc gtcgtcctcg
     2941 ccggcagcct ggccgcccag gtcctcgtcg ggacctgggg ctccttcggg gccatgccgg
     3001 aggcctggag ggcggccttt tattccgccg ccgccatcgt cttcctggtc gggggcggga
     3061 tgaagtgggt cctgttcaac cggagggcca ggctcataag gcgggaggcc aactacctaa
     3121 ccgtcatcaa ggccttctac gcgggatcct ggttccacat caacgtgccc agctacctct
     3181 gcgccctggc cgggatcggc ttcgcgatag ccctgggcca cccctggtac gccctgccgg
     3241 tctccttcgt gttcatagcc ctgctgttca acaacctcgg ctcggggatc cagcgccccg
     3301 agtatctggt ctcgggctgg tacgccctgg tgacggccct cgcctccttc ctcttcgtcg
     3361 agcgggcccc cttcgtctgg ctggccgtcg tgctcggggg cttctgcctc gtcttcgccg
     3421 tcaccggcct cctcggccgc gacgggggag gagagcgatg agcccggcct ccg
//
LOCUS       DASSQV010000370         4022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1951706, whole genome shotgun sequence.
ACCESSION   DASSQV010000370 DASSQV010000000
VERSION     DASSQV010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951706"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFL04_06455"
     CDS             <1..460
                     /locus_tag="VFL04_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7839383.1"
                     /translation="GDVVEEIRTLSAQTNLLALNATIEAARAGEAGKGFAVVAGEVRK
                     LAEHSQSAAGEIAELSARSVAVAERTGELLARIVPDIQRTASLMQEIAAASREQSNGA
                     DHVTKAIGQLDAVIQANSAASEELAASSEELSVQAIALQETLSFFKTSQV"
     gene            610..2703
                     /locus_tag="VFL04_06460"
     CDS             610..2703
                     /locus_tag="VFL04_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HET7839384.1"
                     /translation="MTKPLAPVIRVIDDNCVNCHNCIAVCPVKYCIDGSGEKVRIRHE
                     LCIGCGSCIEGCTHKAREGIDDTEAFFQALGRGEPMVAIVAPAIAGRLGPAFLRLNGW
                     LRASGVQAVYDVAFGAELTVKSYEEHLKAADPAMVIAQPCPAIVNYIEIYQPELAKYL
                     APADSPMLHTIKMLREWRPELKACRVAVISPCVAKRREFDETGLGDYNVTIERLLPEL
                     AKRKVELSSFPELDFDNPPAETAVLFSSPGGLKATVERDFPELANRIRRVEGPRNVYP
                     YFQDLGKSVAEGMQPKIVDCLNCDKGCNGGTGTGSAHLPVDILEGRIAARRLVQQRRI
                     AGASPKPEKSARLLRRSLEAFWKPGMYKRDYADRRRALDLRIPDEADFRRIYAAMLKL
                     EEEDFLNCAACGYNSCEMMAVAIHNGLNKAENCHHYQREVIVRSHESLLATSRRLDGE
                     IARTVVMIEQLLGMLPELETRSQEESAGLEESTRTISAMLATLSRSAALSETGKGDLG
                     LLAAGVRDSERSLDSSIAAIKGASAQVGGINEMVDEIDKIAEQTNLLSMNAAIEAAHA
                     GEAGKGFAVVAEEIRRLSEQASDSSHNIALTLGELVKTITEASGLSDGAGAAVREMLN
                     RVTGTAAGLEEIFQSLTSMSVGTAQVNAALSTLGTSVGSVRELYREMQGIISKVGEEV
                     RAISAISKANVGEGA"
     gene            complement(2700..3074)
                     /locus_tag="VFL04_06465"
     CDS             complement(2700..3074)
                     /locus_tag="VFL04_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7839385.1"
                     /translation="MRARIMIVEDEAIPALFLRRLLEGEGFVVCAVLAEGELVLASFA
                     ETRPDLILMDIRLSGRMDGIQVAEELRRESSVPIVFVSGYEGGPLHERALATFPVAFL
                     GKPVRREALLEAIGAALGRLGA"
     gene            complement(3080..>4022)
                     /locus_tag="VFL04_06470"
     CDS             complement(3080..>4022)
                     /locus_tag="VFL04_06470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7839386.1"
                     /translation="AEANRSIGSALDELVARLEAQAGGAPDGPGGRVGIRIAYYEIQE
                     RAERLDLELRGLMARRLATLDLEYLAALWLTALLLVLAATVIWLRTRREAAILGELRA
                     ANAEQEVLLRELNHRTKNNMNVISSWLGLESMRSADPAVRAVLHEADARIQAMALVHS
                     KLYQGGKLSSLDLGEYLRELLGMLREAYASGREGIRVEGELESVTTSIDAALPCGLIV
                     NELFSNSLKHAFPDGRPGRIAVALRRGGGGEIELSYSDDGVGCPEGFSARSQSGLGLR
                     TVVAIGEDQLRGRLAFATRGGFACSLSFGDADFRPRV"
BASE COUNT          655 a         1463 c         1383 g          521 t
ORIGIN      
        1 cggcgacgtc gtggaggaga tccgcaccct ctcggcccag accaacctcc tcgccctgaa
       61 cgccaccatc gaggccgcgc gggcgggcga ggccggcaag ggcttcgccg tcgtggccgg
      121 cgaggtgcgc aagctcgccg agcactcgca gagcgccgcc ggagagatcg ccgagctgtc
      181 ggcccgctcg gtggccgtgg ccgagcgcac gggcgagctc ctcgcgagga tcgtgcccga
      241 catccagagg acggcctccc tcatgcagga gatcgccgcg gcgagcaggg agcagagcaa
      301 cggggccgac cacgtgacca aggccatcgg ccagctcgac gccgtcatcc aggccaactc
      361 cgccgcctcc gaagagctcg ctgcctcctc cgaggagctc tcggtccagg ccatcgccct
      421 ccaggagacc ctgtccttct tcaagacctc acaggtgtga cgaagacggc gggcgcctgc
      481 ggcccgcatg cgggcagggg gctgccgcgc gctcggcttg acgccgggtg gccccattgg
      541 cgatagtgga gcgaagaaat aatcgttgcg gccgctgcac gaacaccgcg gcccgccaag
      601 gaatgcgcca tgaccaagcc cctcgctccc gtcatcaggg tgatcgacga caactgtgtc
      661 aattgccaca actgcatagc cgtatgcccc gtcaagtact gcatcgacgg ctcgggcgag
      721 aaggtgagga tccgccacga gctgtgcatc ggctgcggct cctgcatcga gggctgcacc
      781 cacaaggcgc gcgaggggat cgacgacacc gaggccttct tccaggccct cgggcgcggg
      841 gagcccatgg tcgccatcgt cgcccccgcc atcgcgggca ggctcgggcc ggccttcctc
      901 cggctcaacg gctggctcag ggccagcggg gtccaggcgg tctacgacgt ggccttcggc
      961 gccgagctca ccgtcaagtc ctacgaggag cacctgaagg cggccgaccc cgccatggtg
     1021 atcgcccagc cctgccccgc catcgtcaac tacatcgaga tctaccagcc cgagctcgcg
     1081 aagtacctgg cccccgccga cagccccatg ctccacacca tcaagatgct ccgcgagtgg
     1141 aggcccgagc tcaaggcctg cagggtcgcc gtgatctcgc cctgcgtggc caagcgccgc
     1201 gagttcgacg agactggcct gggcgactac aacgtcacca tcgagcgcct cctgcccgag
     1261 ctggccaagc gcaaggtcga gctctcctcc ttccccgagc tcgacttcga caaccccccg
     1321 gccgagaccg ccgtcctctt ctcgagcccc ggcggcctca aggccaccgt ggagcgcgac
     1381 ttccccgagc tcgcgaaccg gatccgccgg gtcgaggggc cccgcaacgt ctacccctac
     1441 ttccaggacc tcggcaagtc ggtcgccgag ggcatgcagc cgaagatcgt cgactgcctc
     1501 aactgcgaca agggctgcaa cggcgggacc ggcacgggct cggcccacct ccccgtcgac
     1561 atcctcgagg gccgcatcgc cgcgcgcagg ctcgtccagc agcgccgcat cgccggggcg
     1621 agccccaagc ccgagaagtc ggccaggctc ctgcgccgga gcctcgaggc cttctggaag
     1681 cccggcatgt acaagcgcga ctacgcggac cgccgccggg ccctcgacct ccgcatcccc
     1741 gacgaggccg acttcaggcg catctacgcc gccatgctca agctcgagga ggaggacttc
     1801 ctcaactgcg cggcctgcgg ctacaacagc tgcgagatga tggcggtcgc catccacaac
     1861 ggcctcaaca aggccgagaa ctgccaccac taccagcgcg aggtgatcgt gcggagccac
     1921 gagagcctcc tcgccacctc gcgccggctc gacggcgaga tcgccaggac cgtcgtgatg
     1981 atcgagcagc tcctcggcat gctgcccgag ctcgagacga ggagccagga ggagtcggcc
     2041 gggctcgagg agtccacccg cacgatcagc gcgatgctcg cgaccctgtc gcgcagcgcg
     2101 gccctctcgg agacgggcaa gggggacctc ggcctcctcg ccgccggggt gcgggacagc
     2161 gagcgcagcc tcgactcctc catcgcggcc atcaagggcg cgagcgccca ggtgggcggc
     2221 atcaacgaga tggtcgacga gatcgacaag atcgccgagc agacgaacct cctgtccatg
     2281 aacgccgcga tcgaggcggc ccacgcgggc gaggccggca agggcttcgc cgtcgtggcc
     2341 gaggagatcc gccgcctctc cgagcaggcg agcgacagct cgcacaacat cgccctcacc
     2401 ctgggcgagc tcgtgaagac catcaccgag gcctcgggcc tctccgacgg ggcgggcgcc
     2461 gccgtccgcg agatgctcaa ccgggtcacc gggaccgcgg ccgggctcga ggagatcttc
     2521 cagtcgctca cgagcatgtc ggtgggcacc gcacaggtga acgccgccct ctcgaccctc
     2581 gggacctcgg tcgggagcgt gcgcgagctc tacagggaga tgcaggggat catctcgaag
     2641 gtgggcgagg aggtccgcgc gatctccgcg atctccaagg ccaacgtggg ggagggggcc
     2701 taggctccga gtctccccag cgccgccccg atcgcctcga ggagggcctc gcgccgcacc
     2761 ggcttgccga ggaaggccac ggggaaggtg gccagggcgc gctcgtggag ggggccgccc
     2821 tcgtagcccg agacgaagac gatcggcacc gaggactcgc ggcggagctc ctcggccacc
     2881 tggatcccgt ccatccgccc cgagagcctg atgtccatga ggatgaggtc gggcctggtc
     2941 tcggcgaagg aggcgaggac cagctcgccc tcggccagga cggcgcagac cacgaagccc
     3001 tcgccctcga gcaacctgcg caggaagagc gcgggtatgg cctcgtcctc gacgatcatg
     3061 atcctagcgc gcatggccgc tagaccctcg gcctgaagtc ggcgtcgccg aagctgaggc
     3121 tgcaggcgaa gccgccccgg gtggcgaagg cgaggcggcc gcgcagctgg tcctcgccga
     3181 tggccaccac ggtgcgcagg cccaggccgc tctgggagcg ggccgagaag ccctcggggc
     3241 agcccacccc gtcgtcgctg taggagagct caatctcccc gcctccgccc cgccggagcg
     3301 ccacggcgat cctgccgggc ctgccgtcgg ggaaggcgtg cttgagcgag ttcgagaaga
     3361 gctcgttcac gatgaggccg cagggcaggg ccgcgtcgat cgaggtggtg acgctctcga
     3421 gctccccctc gaccctgatg ccctcccgcc ccgaggcgta ggcctcgcgc agcatgccca
     3481 ggagctcgcg caggtactcg ccgaggtcga ggctcgagag cttgcccccc tggtagagct
     3541 tgctgtggac gagggccatg gcctggatgc gggcgtcggc ctcgtggagg acggcgcgga
     3601 ccgccggatc ggcgctccgc atggactcga gcccgagcca ggagctgatc acgttcatgt
     3661 tgttcttggt gcggtggttg agctcgcgga ggaggacctc ctgctccgcg ttggccgcgc
     3721 gcagctcgcc caggatggcc gcctcccgcc tcgtgcggag ccagatcacc gtggccgcga
     3781 gcacgaggag gagggcggtg agccagagcg cggccagata ctcgaggtcg agggtggcga
     3841 ggcgccgggc catgaggccc cgcagctcga ggtcgaggcg ctcggcccgc tcctggatct
     3901 cgtagtaggc gatgcggatc cccaccctgc ccccggggcc gtcgggggcg cccccggcct
     3961 gggcctcgag ccgggccacg agctcgtcca gggccgagcc gatcgaccgg ttggcctcgg
     4021 cg
//
LOCUS       DASSQV010000371         4678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_461559, whole genome shotgun sequence.
ACCESSION   DASSQV010000371 DASSQV010000000
VERSION     DASSQV010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461559"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFL04_06475"
     CDS             <1..706
                     /locus_tag="VFL04_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7839387.1"
                     /translation="HGGPAGVHGEAIFMWESYGTHFIDYTGGKYKVVLDEKKATEGME
                     FYKKLMAIAPKGAETMAHVERQAAFAADPKGVFSMFIWPAQISAYEDPDKSLVAGKIL
                     YSAPPAGPAKTVAVRGTWALAIPAASKQQAAAAEFVYWWASKDIASRLVEANTIPARS
                     DALADPKFANKPWLAAIAESMKYATARPRLKEIPEIQDIVKKYWIQGITGQIPCADAV
                     KAIVKETNAVLAKAGY"
     gene            730..1707
                     /locus_tag="VFL04_06480"
     CDS             730..1707
                     /locus_tag="VFL04_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7839388.1"
                     /translation="MEIQSALPAASPGRRAPGPGAALLDRAGIRRAEAPPLLFLLPAL
                     VIVFAVLIFPILFSLYVSFFDWPLSSGAGEPRFLGLGNYAALSLDPEFWKSLRLQLGF
                     IFIAIPVELFLGFCAALLLNREFFGARIVRTLLLLPVFFLPIVSGMTWRFMLQPRYGP
                     LNALLIALGIPEMTWLNDPIFAYVAIVAQDIWRMWPFMFMILYAGLTNVPSELLEAAE
                     IDGASYWRRLRSVVVPLLVPTILTALLLRIIDALRVFSEVYVMTEGGPGSATLLFSLY
                     TYRQGFSYMRVGMASAMAIFLLILSVVFAMTLVRRNMSLDELERKAGGR"
     gene            1704..2546
                     /locus_tag="VFL04_06485"
     CDS             1704..2546
                     /locus_tag="VFL04_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7839389.1"
                     /translation="MTAIARRRRRAAMTALIAIGAFLVLAVELYPIAITLLNGFRKDI
                     VILSGRPFAFSQLSLRSYELVLRNPGFLLGMKNSVLVGLLATFVSVLAGAMASYGISR
                     YRFRGRDGLAYGFLVFRMLPQISLVIALYLMFRMAHIRDTVFGLCLAYASFNVPYVIW
                     MLLPFFAAIDKSYEEAARVDGCSPFGVFARVFLPLAAPGLVVSAVFAFINCWNEFLYA
                     LILTGVNAKTAPIAINGLIGGETLTWGQACAAGTLMLVPVFVFTVTMQRFLVRGISAG
                     GVKG"
     gene            2553..4616
                     /locus_tag="VFL04_06490"
     CDS             2553..4616
                     /locus_tag="VFL04_06490"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET7839390.1"
                     /translation="MESFILRERPEGFEIEAEGRTIIRHTRGEPFIRAGRGEGRFEMY
                     RGNFEISERLDELVALRDFEIGNRAVGREGLVSLELRFSRSGDYALSSRCAIEEGRLV
                     VGFSPLGEAVAAANRWRLSLPAEPAERVYGCGEQFSYLDLRGREFPIWTSEQGVGRNK
                     ASLVTWQADVEGRAGGDYWWTFFPQPSFVSSRRLWFHLNTSAYARFDFRSPERHELVS
                     WALPDSIVIGAADTMGELVSDLSAWFGRQGGLPDWCYDGVILGMQGGTEACMSRLAAA
                     REAGVPVAGIWAQDWEGVNHTSFGQRLRWNWVWDRERYPGLDAAIRRLSDEGVRFLGY
                     INPYVGAGMSLFNEASARGFLAKNRGGEDYLVDFGEFEAGIVDFTNPEAFEWYKTVIG
                     RELLDLGLSGWMADFGEYLPTDAVLSDGTSAEIAHNAWPAIWARCNREAVDASDRASE
                     VLYFMRAGGPGSQLCCPMMWAGDQNVDWSDDDGLPSVITAALSAGLSGHGLNHSDIGG
                     YTSLYGMRRTKELFMRWAEQAALSPLMRSHEGNRPKENWQFDSDPGTLAHLARMGRLH
                     VALAPYLRAAVAENSARGLPVMRPLFLGHEDDEAAWTIKDQYLLGGELLVAPVIREGA
                     TSRRVHLPKGTWVHAWSGGRLGGGEALDLEVGAGLGEPPFFWREASSWAGLFADAVKR
                     ARAEA"
BASE COUNT          676 a         1698 c         1499 g          805 t
ORIGIN      
        1 ccacggcggg ccagccggcg tccacggcga ggccatcttc atgtgggagt cctacggcac
       61 ccacttcatc gactacacgg gcgggaagta caaggtcgtc ctcgacgaga agaaggccac
      121 cgaaggcatg gagttctaca agaagctcat ggccatcgcg cccaaggggg ccgagaccat
      181 ggcccacgtc gagcgccagg ccgccttcgc cgccgacccc aagggcgtct tctcgatgtt
      241 catctggcca gcccagatct cggcctacga ggatcccgac aagtccctcg tcgccggcaa
      301 gatcctctac tccgccccgc ccgcgggccc ggccaagacg gtcgccgtgc gcgggacctg
      361 ggccctcgcc atccccgccg cctccaagca gcaggcggca gcggccgagt tcgtctactg
      421 gtgggcctcc aaggacatcg cctcgaggct cgtcgaggcc aacaccatcc ccgcccgaag
      481 cgacgccctc gccgacccga agttcgcgaa caagccctgg ctggctgcca tcgccgagtc
      541 catgaagtac gcgaccgcga ggccccggct caaggagatc cccgagatcc aggacatcgt
      601 gaagaagtac tggatccagg gcattaccgg ccagatcccc tgcgccgacg cggtcaaggc
      661 catcgtcaag gagaccaacg ccgtcctggc caaggcggga tactgacgcg gccctggatg
      721 agcgggggaa tggagatcca gagcgccctg cccgccgcct ccccggggcg gcgggctccg
      781 gggcccggcg cggccctgct ggaccgggcc gggatccgcc gcgccgaggc tccacccctg
      841 ctcttcctcc tgccggcctt ggtcatcgtc tttgcagtcc tgatcttccc catactcttc
      901 tccctctacg tctccttctt cgactggccc ctgtcctcgg gggctggcga gcccaggttc
      961 ctcggcctcg gcaattacgc ggccctctcg ctggaccccg agttctggaa gagcctgagg
     1021 ctccagctcg gcttcatctt catcgcgatc cccgtcgagc tcttcctcgg cttctgcgcc
     1081 gccctcctcc tgaaccgcga gttcttcggg gccaggatcg tgcggaccct cctcctcctg
     1141 ccggtcttct tcctgcccat cgtctcgggc atgacctggc gcttcatgct ccagccgcgc
     1201 tacggccccc ttaacgccct cctcatcgcc ctcgggatcc ccgagatgac ctggctcaac
     1261 gaccccatct tcgcctacgt ggcgatcgtg gcccaggaca tctggaggat gtggcccttc
     1321 atgttcatga tcctctacgc cgggctcacc aacgtgccga gcgagcttct cgaggccgcc
     1381 gagatcgacg gggcctcgta ctggaggcgg ctgcgcagcg tggtcgtgcc cctcctcgtc
     1441 ccgaccatcc ttaccgcact tctcctgcgc atcatcgacg ccctccgcgt cttcagcgag
     1501 gtctatgtga tgaccgaggg agggcccggt tcggccaccc tgctcttctc gctctacacc
     1561 tacaggcagg gcttctccta catgcgcgtg ggcatggcca gcgcgatggc catcttcctc
     1621 ctcatcctct ccgtggtctt cgcgatgacc ctggtgcgca ggaacatgag cctcgacgag
     1681 ctcgagcgca aggcgggcgg acgatgacgg ccatcgccag gaggcgaagg cgggcggcca
     1741 tgaccgccct gatcgccatc ggcgccttcc tcgtcctcgc ggtcgagctc tatcccatcg
     1801 ccatcaccct gctgaacggc ttccggaagg acatcgtcat cctctccgga cggcccttcg
     1861 cattctccca gctctcgctg cgcagctacg agctcgtcct ccggaacccg ggcttcctcc
     1921 tcggcatgaa gaactcggtg ctcgtgggcc tcctcgccac cttcgtctca gtcctcgcgg
     1981 gggccatggc ctcctacggc atctcgcgtt accgcttcag gggcagggac ggcctggcct
     2041 acggcttcct cgtgttcagg atgctgcccc agatctccct ggtcatcgcc ctctacctca
     2101 tgttcaggat ggcccacatc cgcgacacag tctttggcct ctgcctcgcc tacgcgagct
     2161 tcaacgtgcc ctacgtgatc tggatgctcc tgcccttctt cgccgcgatc gacaagagct
     2221 acgaggaggc tgcgagggtc gacggctgct cgcccttcgg ggtcttcgcc cgggtcttcc
     2281 ttcccctggc ggctcccggc ctcgtggtca gcgccgtctt cgccttcatc aactgctgga
     2341 acgagttcct ctacgccctc atcctcaccg gggtgaacgc caagaccgcc cccatcgcca
     2401 tcaacggcct catcgggggc gagaccctca cctggggcca ggcctgcgcc gccgggaccc
     2461 tcatgctcgt gccggtcttc gtcttcaccg tgacgatgca gcgcttcctc gtccgcggca
     2521 tctcggccgg cggggtgaag ggctagggcg ggatggagag cttcatcctc cgcgaaaggc
     2581 ccgagggctt cgagatcgag gccgagggca ggaccatcat ccgccacacg cgcggcgagc
     2641 ccttcatcag ggccgggcgg ggcgagggcc gcttcgaaat gtaccgcggt aacttcgaga
     2701 tctcggagcg tctggacgag ctcgtggccc tgagggactt cgagatcggc aacagggctg
     2761 tgggccggga gggcctggtt tccctcgagc tccgcttctc gcgttccgga gactacgcgc
     2821 tttcgtcgcg ttgcgcgatc gaggaggggc ggctcgtcgt cggcttctcg cccctgggcg
     2881 aggcggtggc cgcggcgaac cgctggcgcc tctccctccc ggccgagccc gccgagaggg
     2941 tgtacggctg cggggagcag ttctcctacc tcgacctcag gggaagggag ttcccgatct
     3001 ggacgagcga gcagggcgtg ggcaggaaca aggcgagcct ggtgacctgg caggccgacg
     3061 tcgagggcag ggcgggcggc gactactggt ggaccttctt cccccagccc agcttcgtct
     3121 cctcgcggag gctctggttc cacctcaaca cgagcgccta cgcccgcttc gacttccgct
     3181 cgccggagcg tcacgagctc gtctcatggg ccctgccgga cagcatcgtc ataggcgcgg
     3241 ccgacacgat gggcgaactc gtctccgacc tctcggcctg gttcggccgc cagggcgggc
     3301 tcccggattg gtgctacgac ggcgtgatcc tcggcatgca gggcgggacc gaggcatgca
     3361 tgtcccggct cgccgctgcc cgcgaggccg gcgtccccgt ggcggggatc tgggcccagg
     3421 actgggaggg cgtgaaccac acgagcttcg gccagcgcct ccgctggaac tgggtctggg
     3481 acagggagcg ctaccccggc ctcgacgccg cgatccgccg cctctccgac gagggcgtca
     3541 ggttcctcgg ctacataaac ccctacgtcg gcgccggcat gagcctcttc aatgaggcct
     3601 cggcccgcgg cttcctcgcg aagaaccgcg gcggcgagga ctaccttgtc gacttcggcg
     3661 agttcgaggc cggcatcgtc gacttcacca atccggaggc cttcgagtgg tacaagaccg
     3721 tcataggccg ggagctcctg gacctcgggc tctcgggctg gatggccgac tttggcgagt
     3781 acctccccac ggacgccgtc ctctccgacg ggacaagcgc cgagatcgcg cacaacgcgt
     3841 ggccggccat ctgggcccgc tgcaaccggg aggcggtcga cgcgtcggac cgggcctcgg
     3901 aggtcctgta cttcatgagg gcgggtggac cggggagcca gctctgctgt cccatgatgt
     3961 gggcgggcga ccagaacgtc gactggtcgg atgacgacgg cctcccctcg gtcatcaccg
     4021 ccgccctctc cgccggcctt tcgggccatg gcctcaacca cagcgacatc ggcggataca
     4081 cgagccttta tggcatgagg cgcacgaagg agctcttcat gcgctgggcc gagcaggccg
     4141 ccctgagccc cctcatgcgc agccacgagg gcaacaggcc gaaggagaac tggcagttcg
     4201 actccgaccc cgggaccctc gcccacctcg cgcggatggg ccggctccat gtggccctgg
     4261 ctccctatct gcgggccgcg gtggccgaga actccgcgcg ggggctgccg gtcatgcgcc
     4321 cgcttttcct cggccacgag gacgacgagg ccgcatggac gatcaaggac cagtacctcc
     4381 ttggcggcga gctcctcgtg gcccccgtca ttcgtgaggg ggccacgtcg cgcagggtgc
     4441 acctgcccaa ggggacctgg gtgcacgcct ggtcgggtgg gcggctcggc ggcggcgagg
     4501 ccctggacct cgaggtcggg gccgggctcg gggagcctcc cttcttctgg agggaggcct
     4561 cctcctgggc cggccttttc gccgatgcgg tgaaaagggc gcgggcggaa gcctaggaag
     4621 gccgccgccc gcagatcgtc gttatggccg ggctccggag ctcggccggg gccaggcc
//
LOCUS       DASSQV010000372         3897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2202164, whole genome shotgun sequence.
ACCESSION   DASSQV010000372 DASSQV010000000
VERSION     DASSQV010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202164"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VFL04_06495"
     CDS             <1..957
                     /locus_tag="VFL04_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7839391.1"
                     /translation="SFRETLRYKTKAMVGPLVAGRCLVLLPLREASGASESRELLLGI
                     LGSAFRRELAHGYLRLGFGPGKPLEEAASSWAAALAELLGVGEATGQAGGVQADAKPF
                     DDDEAFLEALIGGAPERAGLALDRILEELGPLPVLPAAERYRMISLFGSALRALTRRG
                     FIDRGEAAAMMDLEDLRQAGEGQAFTLAVRSRFHRLSGVMASSPRLTPPVSKAVAFVK
                     ENYGRQLSLELAADAVGLSPNRLSRLFVEETGHGFSDFLIEYRIERAKELLALPGASI
                     KQVSISCGYPDPNYFSRLFKKVTGLTPTAFSSGSTEVSDEKS"
     gene            944..1981
                     /locus_tag="VFL04_06500"
     CDS             944..1981
                     /locus_tag="VFL04_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7839392.1"
                     /translation="MKKAEAPVLLLVLLALLLGLTGCAPRRHRLEPGRVPLIGFSLDS
                     LVVERWQRDIEVFSRSIKDLGAELILRVADQDAGVQEEQVRELVEKGIDVLVIVPNDA
                     DRLSSVVAEVKAKGIQVLSYDRLVRRAGVDLYVSFDNEKVGSLMAEAVARAVPSGGYI
                     IINGARSDYNAIMLNNGFHRVLDPLTKTGRVRIVAEIWPKYWDSDEVKSEMEAVVARD
                     RSIDAVIAGNDMLAEAAIGVLSESRLMGKAKVAGQDAELSACQRIAEGSQYATIYKPI
                     DRLALKAAGFAVMLAKGERIETDAVIDDGAGKVPYVRLEPILVTRPLLASTVIKDGFH
                     EAKDVYRNVNN"
     gene            2182..3264
                     /locus_tag="VFL04_06505"
     CDS             2182..3264
                     /locus_tag="VFL04_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HET7839393.1"
                     /translation="MKRLLVVLSVFLFVAVLAGGVFAQTQVGIVLPTKDEPRWIQDQT
                     RFLDALKTAGISAEVLFSQGDSAKEKANVESLITKGIKVLVICPQDGTAAAAAANEAR
                     QAKVKVISYDRLIRDTAAVDYYVTFDSVQVGAAWGKYLVSKATGKGNNLYLYAGAASD
                     NNAFLFFEGAWSVLQPKIADGTFVVQNSDKAVALSKKAKLSRDEQASIIGQVTTNWNF
                     NDAKSKAEANLTAAPAKAKGTVFIAAPNDGTARAIADAFAADKQVTKYYITGQDAEIA
                     SIQYIIDGKQSMTVLKDVRTLVSDAIAAAVAFIKGQTPAKTTTYDNGKVQVPAKPTAI
                     ITVTKENVKKEIVDSGYWPASNFTGL"
     gene            3370..>3897
                     /locus_tag="VFL04_06510"
     CDS             3370..>3897
                     /locus_tag="VFL04_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7839394.1"
                     /translation="MSTPILQMKSITKTFPGVKALSDVSFSVAKGEIHCLVGENGAGK
                     STLMKVLSGLYPHGDYTGEILFEGRLQKFRGIADSERSGIAIIYQELALVPEMTVYEN
                     ILLGHEIRKGPRVDWNETIKKASEMLGKVRLDINPATKVKDLGVGRQQLVEIAKALSK
                     DVKLLILDEPTAALNE"
BASE COUNT          696 a         1428 c         1198 g          575 t
ORIGIN      
        1 tccttccgcg agaccctccg ctacaagacc aaggccatgg tgggccccct cgtggccggg
       61 cgctgtcttg tcctcctgcc cctgagggag gcctcggggg ccagcgaatc gagggagctc
      121 ctcctcggga tcctgggctc ggccttccgg agggagctgg cccacggcta cctccgcctc
      181 ggcttcggcc cggggaagcc cctcgaggag gcggcctcgt cctgggccgc cgccctcgcc
      241 gagctcctcg gcgtgggtga ggccaccggc caggccggag gcgtccaggc cgacgccaaa
      301 cccttcgacg acgacgaggc cttcctcgag gccctcatcg gcggcgcgcc cgagcgggcg
      361 gggctggccc tcgacaggat cctcgaggag ctcggcccac tgccggtgct ccccgccgcc
      421 gagcgctacc gcatgatctc cctgttcggt tcggccctgc gggcccttac ccgccgcggc
      481 ttcattgacc ggggcgaggc ggccgcgatg atggacctcg aggacctccg ccaggccggg
      541 gagggccaag ccttcacact cgcggtccgc tcgcgtttcc acaggctctc cggggtcatg
      601 gcgagctccc cccgcctgac gcccccggtc tcgaaggccg tggccttcgt gaaggagaac
      661 tacggcagac agctcagcct cgagctcgcg gccgacgccg tcggcctgtc ccccaaccgg
      721 ctctcccggc tcttcgtcga ggagacgggg cacggcttct ccgatttcct catcgagtac
      781 cgcatcgagc gggcgaagga gctcctcgcc ctgcccggcg ccagcatcaa gcaggtgagc
      841 atctcctgcg gctaccccga cccgaactac ttctcgcggc tgttcaagaa ggtcaccggc
      901 ctgaccccga cggccttctc ttccggatcg acggaggtgt ccgatgaaaa aagctgaagc
      961 gcccgtcctg ctcctggtcc tccttgccct cctcctcggc ctcacgggct gcgcgccgcg
     1021 gcgccaccgc ctcgagcccg gccgcgtccc cctcatcggc ttctccctcg actccctggt
     1081 ggtcgagcgc tggcagaggg acatcgaggt cttctcccgc tcgatcaagg acctcggggc
     1141 cgagctcatc ctgcgcgtcg ccgaccagga cgcgggggtc caggaggagc aggtcaggga
     1201 gctcgtcgag aagggcatcg acgtcctcgt catcgtgccg aacgacgccg accggctctc
     1261 ctcggtggtg gccgaggtga aggcgaaggg catccaggtt ctgagctacg acaggctcgt
     1321 gaggagggcc ggggtcgacc tctacgtctc cttcgacaac gagaaggtgg gcagcctcat
     1381 ggccgaggcg gtggcgcgcg ccgtgccctc gggcggctac atcatcatca acggggcccg
     1441 cagcgactac aacgccatca tgctcaacaa cggcttccac cgcgtcctcg accccctgac
     1501 caagacgggc agggtgagga tcgtcgcgga gatctggccc aagtactggg actcggacga
     1561 ggtgaagagc gagatggagg ccgtcgtggc ccgggacagg agcatcgatg ccgtgatcgc
     1621 cggcaacgac atgctcgccg aggcggccat aggcgtcctc tcggagagcc ggctcatggg
     1681 caaggccaag gtggccggcc aggacgccga gctctcggcc tgccagagga tcgccgaggg
     1741 ctcgcagtac gcgacgatct acaagcccat cgaccgcctc gccctcaagg ccgccggctt
     1801 cgccgtcatg ctcgccaagg gcgagaggat cgagaccgac gccgtcatcg acgacggggc
     1861 cggcaaggtg ccctacgtcc gcctcgagcc catcctcgtg accaggcccc tcctcgcctc
     1921 cacggtcatc aaggacggct tccacgaggc caaggacgtc tacaggaacg tgaacaactg
     1981 aagggccctc tggactaatc cccggcctcc gccggggaat agtgctaggc tcccgcccct
     2041 gggcggggcg cagggcgaag tgaagccttt ggccgcaact ttcctgatga ccgtttcctc
     2101 atacgcccga ataatctagg ctatgcggcc gcccgcccca gcgcgggaag ccaggccgcg
     2161 agtctctcaa gggggtatag aatgaaacgt cttctcgtcg tgctttccgt cttccttttc
     2221 gtagcggtcc tcgcgggcgg ggtcttcgcc cagacccagg tcggcatcgt cctccccacg
     2281 aaggatgagc cccgctggat ccaggaccag acccgcttcc tcgacgcgct caagaccgcc
     2341 ggcatcagcg ccgaggtcct cttcagccag ggcgactccg ccaaggagaa ggccaacgtc
     2401 gagtccctga tcaccaaggg catcaaggtc ctcgtgatct gcccgcagga cggcacggcc
     2461 gccgccgccg ccgccaacga ggcccgccag gccaaggtca aggtcatctc ctacgaccgc
     2521 ctcatccgcg acaccgccgc cgtggactac tacgtcacct tcgactcggt ccaggtcggc
     2581 gccgcctggg gcaagtacct cgtcagcaag gccaccggca agggcaacaa cctctacctc
     2641 tatgcgggag ccgcctccga caacaacgcc ttcctcttct tcgagggcgc ctggtcggtc
     2701 ctccagccca agatcgccga cggcaccttc gtcgtccaga actccgacaa ggccgtcgcc
     2761 ctctccaaga aggccaagct cagccgcgac gagcaggcga gcatcatcgg ccaggtcacc
     2821 acgaactgga acttcaacga cgccaagagc aaggccgagg ccaacctgac cgcagccccc
     2881 gccaaggcca agggcaccgt gttcatcgcc gccccgaacg acggcaccgc gcgcgccatc
     2941 gcagacgcct tcgccgccga caagcaggtc acgaagtact acatcaccgg ccaggacgcc
     3001 gagatcgcct cgatccagta catcatcgac ggcaagcagt ccatgaccgt gctcaaggac
     3061 gtccgcaccc tcgtcagcga cgccatcgcc gccgccgtcg ccttcatcaa gggccagacc
     3121 cccgcgaaga ccacgaccta cgacaacggc aaggtccagg tcccggccaa gcccaccgcc
     3181 atcatcacgg tcaccaagga gaacgtgaag aaggagatcg tggactccgg ttactggccc
     3241 gccagcaact tcaccggcct gtaggccggc tctccccaga ctccctatcc ggcgattctg
     3301 cggcgcgcgc gccgcggaat cgcctatact atgccttgac ggcatccttc tcccggaggc
     3361 gagccagcca tgagcactcc cattctccag atgaagagca tcaccaagac cttcccgggc
     3421 gtgaaggccc tgagcgacgt cagcttcagc gtcgccaagg gcgagatcca ctgcctggtg
     3481 ggggagaacg gggccggcaa gtcgaccctg atgaaggtgc tgagcggcct ctatccccac
     3541 ggcgactaca cgggcgagat cctgttcgag ggccgtctcc agaagttccg cggcatcgcc
     3601 gacagcgagc gctcgggcat cgccatcatc taccaggagc tcgccctcgt gcccgagatg
     3661 acggtctacg agaacatcct cctcggccac gagatcagga agggcccgcg ggtcgactgg
     3721 aacgagacga tcaagaaggc gagcgagatg ctcgggaagg tgcggctcga cataaacccc
     3781 gccaccaagg tgaaggacct cggcgtcggc aggcagcagc tcgtcgagat cgccaaggcc
     3841 ctctccaagg acgtcaagct cctcatcctc gacgagccca cagccgccct caacgag
//
LOCUS       DASSQV010000373         3566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1829718, whole genome shotgun sequence.
ACCESSION   DASSQV010000373 DASSQV010000000
VERSION     DASSQV010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829718"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VFL04_06515"
     CDS             <1..1225
                     /locus_tag="VFL04_06515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7839395.1"
                     /translation="LSASFIWSVNTLFLLKAGLDLFGVFLANAVFTAAMCLFEIPTGV
                     FADTRGRKFSFLMCTVFLFVGTLGYASASWFGGGLLAFCLMSVVLGLGFTFYSGAVEA
                     WVVDELKVEGYQGSLDKLFSRGGMIPAASMVLGSVLGGVLGQVNLSLPYVCRSAALFI
                     LFFFALGFMRERGFTPVRGGAQGFWSRAKGIATASAQYGWKVRGVRYLLLEGLSRSSF
                     GIWGFYASQPYLLGKLGDMKAVWLAGAMTAALALSTMAGNSLVGLARRLGGRRTSLLM
                     AAAAISALACLGLSFAGSFVAAFAAILLMTLFQGLFTPVRQAFLNELIPSSQRATVLS
                     FDSLVGDGGAIVGQTGLGWVSKGYGLGAGYLGGSILLAGALPFLLLLRRLGSPADRRA
                     DQEAAAAPAPASSGE"
     gene            1279..1953
                     /locus_tag="VFL04_06520"
     CDS             1279..1953
                     /locus_tag="VFL04_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7839396.1"
                     /translation="MNPVKWIITYGCRFGLRLVLRIDRSELSKVPREGPLILYSNHTG
                     LIEAPILYTELAPRPKVTALSKIENFQKFFLGFVMRVWKVIPIQRGEADMEAMRACLQ
                     SLKEGAILGIAPEGKRSPDGQLQRAQSGVAVLALHSGAPLVPVAHWGGTRMGEHLRRL
                     RREAFVMRVGPVFRLDPKGQRLTREIRQEMADEMMYQLAVLLPESLRGAYADLSKAST
                     KWLRFV"
     gene            complement(1940..2518)
                     /locus_tag="VFL04_06525"
     CDS             complement(1940..2518)
                     /locus_tag="VFL04_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET7839397.1"
                     /translation="MRRLVMWNLVTLDGFFEGAKSWDLGFHEYVWGEELERFAIEQLG
                     EAEALLFGRVTYEGMAAYWSGATGEVAELMNAIPKYGFSRRSGPAPWSNSRLLVGDAA
                     DAVAALKREEGKDLFVFGSALLSHSLMERGLFDEYRLCLVPVVLGAGTPLFKSSAASS
                     RLELMKARPLGSGAVLLYYRPLAEPEAATTRT"
     gene            complement(2557..>3566)
                     /locus_tag="VFL04_06530"
     CDS             complement(2557..>3566)
                     /locus_tag="VFL04_06530"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET7839398.1"
                     /translation="PPLLPPAGIRIFVLGRGRAVGKIAIIGAGAVGATLAYTLTLKGI
                     VSEFALVDTNREKAEAEVLDITHAMPLGHSVKAYASGYEACADAAIVVVTAGAKQRPG
                     ETRVELLGRNAAIARGIVESVYGSGFSGVLLMISNPVDALTYVAHRVAVEGFGAPPQK
                     VIGSGTVLDSARFREFLAGHCDVNPGNIHGYVLGEHGDSSFPAWSLTTIGGVGIDRFC
                     AECGGACETGSLLEEAQASVRQAAEAIIAAKGATYYGIAQAASVIVQAILRNERRILP
                     VSTVRLGYRGIGRTAFSFPTIVGAGGAEKVLDFPLSEEDERKLLASARYVAGVIEGAG
                     L"
BASE COUNT          504 a         1281 c         1188 g          593 t
ORIGIN      
        1 cctctcggcc tccttcatct ggagcgtcaa caccctcttc ctcctcaagg ccggcctcga
       61 cctcttcggc gtcttcctcg cgaacgccgt cttcacggcg gccatgtgcc tcttcgagat
      121 acccacgggg gtcttcgctg acacaagggg gaggaagttc tccttcctca tgtgcaccgt
      181 cttcctcttc gtcggcaccc tcggctacgc ctcggcctcg tggttcggcg ggggcctcct
      241 cgccttctgc ctcatgtcag tggtgctggg cctgggcttc accttctatt cgggcgcggt
      301 ggaggcctgg gtggtcgatg agctcaaggt cgagggatac cagggcagcc tcgacaagct
      361 gttctcccgg ggaggtatga tcccggctgc ctcgatggtc ctgggctctg tcctcggcgg
      421 cgtcctcggc caggtgaacc tcagcctgcc ctacgtctgc cgctcggcgg ccctcttcat
      481 cctcttcttc ttcgccctcg gcttcatgag ggagaggggc ttcaccccgg tccggggcgg
      541 ggcccagggc ttctggtcga gggccaaggg catagccacg gccagcgccc agtacggctg
      601 gaaggtgagg ggggtgcgct acctcctcct cgagggcctc tcgcgctcga gcttcggcat
      661 ctggggcttc tacgcctccc agccctacct cctcggcaag ctcggggaca tgaaggccgt
      721 ctggctcgcg ggggcgatga cggccgccct ggccctctcg accatggcgg gcaacagcct
      781 cgtgggcctc gcccgccgcc tcggcggcag gcggaccagc ctcctcatgg ccgccgcggc
      841 catctcggcc ctcgcctgcc ttggcctctc cttcgcgggc agcttcgtcg cggccttcgc
      901 cgccatcctc ctcatgaccc tctttcaggg cctcttcacg ccggtgaggc aggccttcct
      961 caacgagctc atacccagct ctcagcgggc gacggtcctc tccttcgact ccctcgtggg
     1021 cgacgggggg gccatcgtgg gccagaccgg gctcggctgg gtgtccaagg gctacggcct
     1081 gggcgcgggc tatcttggcg gctccatcct cctcgcgggc gccctgccct tcctcctcct
     1141 tcttaggagg ctcgggtcgc ccgccgacag gcgggccgac caggaggccg cggcggcccc
     1201 tgcccccgcg tcctcggggg agtgagacag cccgaaaccc gtgccctttc gcctgaatcc
     1261 gtgctagcat gtcgtggcat gaatcccgtc aaatggatca tcacctacgg ctgcaggttc
     1321 gggttgcggc tcgtgctcag gatcgaccga agtgagctgt ccaaggtccc ccgcgagggc
     1381 ccactcatcc tctactcgaa ccacacgggc ctcatcgagg ccccgatcct ctacaccgag
     1441 ctggcgcccc ggcccaaggt caccgccctc tccaagatcg agaacttcca gaagttcttc
     1501 ctcggcttcg tcatgagggt ctggaaggtg atcccgatcc agcgcggcga ggccgacatg
     1561 gaggcgatgc gcgcctgtct gcagagcctc aaggagggcg ccatcctcgg catcgccccc
     1621 gaggggaaga ggagccccga cgggcagctc cagcgggccc agagcggagt cgccgtcctc
     1681 gccctccatt ccggcgcccc cctcgtgccg gtggcccact ggggtgggac caggatgggc
     1741 gagcacctca gacgcctcag gcgggaggcc ttcgtcatgc gggtcgggcc cgtgttccgc
     1801 ctcgacccca aggggcagag gctgacccgg gagatcagac aggagatggc cgacgagatg
     1861 atgtaccagc tcgccgtcct cctgcccgag tccctccgcg gggcctatgc cgacctctcg
     1921 aaggcgagca ccaagtggct caggttcgtg tagtggcggc ctcgggctcc gccagggggc
     1981 ggtagtagag aaggacggcc cccgatccca gggggcgggc cttcatgagc tcaagcctcg
     2041 acgatgcggc cgagctcttg aacaggggag tgccggcccc caggacgacg gggaccagac
     2101 agagccggta ctcgtcgaag agcccgcgct ccatgaggga atgcgagagc agggcgctcc
     2161 cgaagacgaa gaggtccttg ccctcctccc gcttgagggc ggcgacggca tcggctgcgt
     2221 caccgacgag gaggcggctg ttgctccacg gggctggccc gctccgcctc gagaagccgt
     2281 atttcgggat cgcgttcatg agctcggcca cctcgcccgt ggccccgctc caataggccg
     2341 ccatgccctc gtaggtcacc cggccgaaga ggagggcctc cgcctcgccc agctgctcga
     2401 tcgcgaagcg ctcgagctcc tcgccccaga cgtactcgtg gaagccgagg tcccagctct
     2461 tcgccccctc gaagaatccg tcgagagtga cgaggttcca catcacaagc cttctcaccg
     2521 catgcctccg cgcgctcatg gcgcgccccg cgctggctag agcccggcgc cctcgatcac
     2581 cccggcgaca tagcgcgcag aggccaggag cttccgctcg tcctcctccg agagggggaa
     2641 gtcgaggacc ttctcggccc cgccggctcc cacgatggtc gggaagctga aggccgtcct
     2701 cccgatgccc cggtagccga gccgcaccgt cgagaccggg aggatgcggc gctcgttgcg
     2761 caggatggcc tggacgatca ccgaggcggc ctgggcgatg ccgtagtagg tggcgccctt
     2821 ggccgcgatg atggcctcgg cggcctggcg cacgctggcc tgggcctcct cgaggaggga
     2881 accggtctcg caggctccgc cgcactcggc gcagaagcgg tcgatgccca ccccgccgat
     2941 cgtggtcagg gaccaggcgg ggaaggagga gtcgccgtgc tcgccgagga cgtagccgtg
     3001 gatgttcccc gggttgacgt cgcagtgccc ggccaggaat tccctgaacc gcgccgagtc
     3061 gaggaccgtg cccgagccga tgaccttctg gggcggggcc ccgaagccct ccacggcgac
     3121 gcggtgggcg acataggtga gcgcatctac cgggttcgag atcatgagga ggacgcccga
     3181 gaagcccgag ccgtagaccg actccacgat gccgcgggcg atggccgcgt tgcggccgag
     3241 gagctcgacc ctggtctcgc cggggcgctg tttggccccg gccgtcacca ccacgatggc
     3301 cgcgtcggcg caggcctcgt agcccgaggc gtaggccttc acgctgtggc cgaggggcat
     3361 cgcgtgggtg atgtcgagga cctcggcctc ggccttctcc cggttcgtgt cgacgagggc
     3421 gaactcgctc acgatgccct tcagggtcag ggtgtaggcg agggtggccc ccaccgcgcc
     3481 cgccccgatg atcgctatct tgcccacggc ccgccccctt cccagaacga atatcctgat
     3541 tccggcgggc gggagcaagg gcggcc
//
LOCUS       DASSQV010000374         4312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_959290, whole genome shotgun sequence.
ACCESSION   DASSQV010000374 DASSQV010000000
VERSION     DASSQV010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959290"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="VFL04_06535"
     CDS             <1..1349
                     /locus_tag="VFL04_06535"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7839399.1"
                     /translation="AGKNGYALLAADVSGRGVAVALLTGLLSAIAARSRPKPGPRAEV
                     DAATAPGQAGPASAEAVKEAEARLAAAEAKANEQTGSLNAALSELNEIKDGKRLELRM
                     SGRVQRRVVPRTDELPSRPEISFNALYAPSENTGGDIFDVVRAGKNGYALLAADVSGR
                     GVAVALLSALVKNAFRSRAAWGIDPATALAEVNAELVPVIGGSEHYVSAFFGFLNLET
                     GELAYSVAGHPACMLLHRRNSEIEDLSGPDPALGVIEEAEFHAEEASLEEGDRILLFT
                     DGVTGARNYQGEAFGRDRLAQVFTGCKDSSLAALPPAILEGINEFTIGSPHSDDIVAL
                     ACEFRSFARDESANRRLLPDSDDYLSLSRRGAELAAAGKIEEAIRVYQRVLMLEPEDA
                     KALNNIGTLFWRMGRRDKAAEKFKAAARLDPNDPRITRNLALVQRGSGKDEGTGEV"
     gene            1351..2154
                     /locus_tag="VFL04_06540"
     CDS             1351..2154
                     /locus_tag="VFL04_06540"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7839400.1"
                     /translation="MEIDSLAYRTDLTLAKAEGEVADRGDYLVVTTPSNPGFHWGNYL
                     LFKRGPQDGDLARWEAIFDREIGCRLKVEHYAFGWDQTGPEPGCVGQVLAKKYNLNRS
                     VVLTAREAHLPPKYNDEAVVRALRTDSEWGQALENQIACNDEDHEPRSFRAFKTRQME
                     AYRRMAACGQGWWFGAFIGDRLVADLGIFVTGRTARFQNVETCREHRREGICGTMVYE
                     SSRRAAELAGIDEFVMVADEDYHAARIYESVGFLPREHQYGLDWWKRGA"
     gene            2151..2831
                     /locus_tag="VFL04_06545"
     CDS             2151..2831
                     /locus_tag="VFL04_06545"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7839401.1"
                     /translation="MSVYLVRHADKEEGEHYGPELRLNDQNLSEAGRTQALRLLDCFD
                     GIGIDTIRASRYRRTSQTASFLAHERGIGIRVDPRLDEIDIGELDRLKDDEIRVRYPD
                     FWRAYTERTADFRFPGGESGEEAGARIHEAFLELDLEGNHLLVAHDGIIRALLCRVLG
                     LPAHRRHLFRIGLCSVTRLEYQSDFGSWAIAEMNGTAHLRERCGPGAASTRREWETWL
                     KTGGMLDD"
     gene            2942..3313
                     /locus_tag="VFL04_06550"
     CDS             2942..3313
                     /locus_tag="VFL04_06550"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7839402.1"
                     /translation="MADKPDYKLHMDIKYDYLEKISVPDIVRDCRDKWFNQTLCKVNG
                     SVLRIGIFEGEFHMHKHDHDDEVFFVLEGELTLETEHGNFDLRRFEGVCVPKGVMHRP
                     LSPTKSVVLMIENEGIRPTGD"
     gene            3313..3849
                     /locus_tag="VFL04_06555"
     CDS             3313..3849
                     /locus_tag="VFL04_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7839403.1"
                     /translation="MPEGSTEGLERFAIPGVGGIIERIVDGEKSILVQERHKAGAKAE
                     EGMIEIPAGKIREGESIFDCLRREIGEETGLIVSRIEGEPESVSTTINGYRVLSYTPF
                     SSAQNLAAGYPIMVQVFFCRVGAEARLLKQSDESRNLRWVGIDELRALLEAPSRLYPM
                     HIETLRRYCELELVRRAR"
     gene            3874..>4312
                     /locus_tag="VFL04_06560"
     CDS             3874..>4312
                     /locus_tag="VFL04_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HET7839404.1"
                     /translation="MELRVYQVDSFTTELFKGNPAGVVPNADGLSEALMQQIARELNN
                     SETAFSINSGGEGYDVEVRFFTPTREVPICGHATVATHYVYARERGIRSGIIRQKTKA
                     GILPVEILPEDDDLRDVMTQAGIEYGAILGGEEREALLQGLGIG"
BASE COUNT          778 a         1506 c         1430 g          598 t
ORIGIN      
        1 gggctggcaa gaacggctac gccctcctcg ccgccgacgt ctcgggcagg ggcgtggcgg
       61 tggccctcct cacgggcctg ctctcggcca tcgcggcccg ctcccgcccc aagccggggc
      121 cgcgcgccga ggtggacgcc gctacagccc ccgggcaggc cggcccggcc agcgccgagg
      181 ccgtcaagga ggccgaggcc aggctcgccg cggccgaggc caaggcgaac gagcagacgg
      241 gctcgctcaa cgccgcgttg agcgagctca acgagatcaa ggacgggaag cggctcgagc
      301 tccgcatgtc gggcagggtc cagaggcggg ttgtgccaag gaccgacgag ctgccctcaa
      361 ggcccgagat atcgttcaac gccctctacg ccccatcgga gaacaccggg ggcgacatct
      421 tcgacgtggt cagggctggc aagaacggct acgccctcct cgccgccgac gtctcgggca
      481 ggggcgtggc ggtggccctc ctctcggccc tcgtgaagaa cgccttccgc tcccgcgccg
      541 cctggggcat cgaccccgcc accgccctcg ccgaggtgaa cgccgagctc gtccccgtca
      601 tcggggggag cgagcactac gtctcggcct tcttcggctt cctcaacctc gagaccggcg
      661 agctcgccta ctcggtcgcc ggccacccgg cctgcatgct cctgcaccgc cgcaacagcg
      721 agatcgagga cctctcggga cccgaccccg ccctgggcgt gatcgaggag gccgagttcc
      781 acgccgagga ggccagcctc gaggagggcg accggatcct cctcttcacc gacggcgtga
      841 ccggggccag gaactaccag ggcgaggcct tcggccgcga ccgcctggcc caggtcttca
      901 ccggctgcaa ggactcgagc ctcgccgccc tgcccccagc gatcctcgag ggcatcaacg
      961 agttcacgat cggctccccc cactccgacg acatcgtcgc cctcgcctgc gagttccgct
     1021 ccttcgcccg cgacgagagc gccaaccgca ggctcctgcc cgactccgac gactacctga
     1081 gcctctcgcg ccggggggcc gagctcgccg cggccggaaa gatcgaggag gccatcaggg
     1141 tctaccagcg ggtcctcatg ctcgagcccg aggacgcgaa ggccctgaac aacatcggca
     1201 ccctcttctg gaggatgggc cgcagggaca aggccgccga gaagttcaag gccgcggcgc
     1261 ggctcgatcc gaacgacccg cgaatcacca ggaacctcgc cctcgtccag cggggatcgg
     1321 gcaaggacga gggcacaggg gaggtctgag atggagatcg actcgctcgc ctaccgcacc
     1381 gacctcaccc tcgccaaggc cgagggcgag gtggccgacc gcggcgacta cctcgtcgtg
     1441 acgaccccga gcaaccccgg cttccactgg ggcaactacc tccttttcaa gcgggggccc
     1501 caggacggag acctcgcgcg ctgggaggcc atcttcgacc gcgagatcgg ctgcaggctc
     1561 aaggtcgagc actacgcctt cggctgggac cagacgggtc ccgagcccgg ctgcgtgggc
     1621 caggtcctcg cgaagaagta caacctgaac cgctcagtgg tcctcaccgc ccgcgaggcg
     1681 catctgcctc cgaagtacaa cgatgaggcc gtggtgcgcg cgcttaggac cgactccgag
     1741 tggggccagg ccctcgagaa ccagatagcc tgtaatgacg aggaccacga gccccgctcc
     1801 ttccgggcct tcaaaaccag acagatggag gcctaccgcc gcatggccgc gtgcggccaa
     1861 ggatggtggt tcggcgcctt catcggggac cgcctcgtgg ccgacctcgg gatcttcgtg
     1921 accgggagga cggcccgttt ccagaacgtc gagacctgca gggagcaccg gcgcgagggc
     1981 atctgcggga ccatggtcta tgagtcgagc cggagggccg ccgagctcgc ggggatcgac
     2041 gagttcgtca tggtcgccga cgaggactac cacgcggcca ggatctacga gtccgtgggc
     2101 ttcctccccc gcgagcacca gtacggcctc gactggtgga agcggggagc atgagcgtct
     2161 acctcgtgcg ccacgccgac aaggaggagg gcgagcacta cggccccgag ctcaggctca
     2221 acgaccagaa cctcagcgag gccgggagga cccaggccct gaggctcctc gactgtttcg
     2281 acggcatcgg catcgacacg atccgcgcga gccgctaccg aagaacctcg cagacggcct
     2341 ccttccttgc gcatgagcgg ggcatcggca tcagggtcga cccgaggctc gacgagatcg
     2401 acatcggcga gctcgacagg ctgaaggacg acgagatcag ggtccgctac cccgatttct
     2461 ggcgggccta caccgagagg actgccgact tccgcttccc cggcggcgag agcggggagg
     2521 aggccggcgc gaggatccac gaggccttcc tcgagctgga tctcgagggg aaccacctcc
     2581 tcgtggccca cgacggcatc atcagggccc tcctctgccg ggtcctcgga ttgcccgccc
     2641 atcgacgcca cctcttccgg atcgggctct gctcggtcac caggctggaa taccagagcg
     2701 acttcggctc ctgggccatc gccgagatga acggcactgc ccacctcaga gaacgctgtg
     2761 gccctggcgc ggcgtccacg cgccgcgaat gggagacctg gctcaagacc ggcggcatgc
     2821 tcgacgacta aagagggcgc gagcttgacc ccgcggcgcc tccgtggcta ccatcgcgca
     2881 aggtgagccg cgacacagcc aacgtctgct ggtataagcg ggatcacccg gggaggcgcg
     2941 catggccgat aagccagact acaagctcca catggacatc aaatacgact acctcgagaa
     3001 gatcagcgtc cccgacatcg tgcgggactg cagggacaag tggttcaacc agaccctctg
     3061 caaggtcaac ggctccgtcc tcaggatcgg gatcttcgag ggcgagttcc acatgcacaa
     3121 acacgaccac gacgacgagg tcttcttcgt cctcgagggc gagctcacac tcgagaccga
     3181 gcacggcaac ttcgatctca ggcgcttcga gggggtctgc gtccccaagg gcgtcatgca
     3241 caggcccctc tcgcccacga agtcggtggt cctcatgatc gagaacgagg gcatcaggcc
     3301 cacgggggac tgatgcctga ggggtcgacg gaagggctcg agcgcttcgc gatccccggc
     3361 gtgggcggga tcatcgagag gatcgtcgat ggcgagaagt ccatcctcgt ccaggagcgg
     3421 cacaaggccg gggcgaaggc cgaggagggg atgatcgaga tccccgcggg caagatcagg
     3481 gagggcgaga gcatcttcga ctgcctgcgg cgggagatcg gggaggagac cggcctcatt
     3541 gtctcccgca tcgaggggga gcccgagtcg gtcagcacga ccatcaacgg ctacagggtc
     3601 ctcagctaca cgcccttctc gtcggcgcag aacctggccg cgggctaccc gatcatggtg
     3661 caggtcttct tctgtagggt cggggccgag gcccggctcc ttaagcagag cgacgagtcc
     3721 aggaacctgc gctgggtcgg gatcgacgag ctcagggccc tgctcgaggc cccgagccgg
     3781 ctctacccga tgcacatcga gaccctgcgc aggtattgcg agctcgagct ggttcggcgc
     3841 gcgcgttagc tcttcggcta ggagggaagc atcatggaat tgcgggtcta ccaggtcgac
     3901 tccttcacga ccgagctctt caagggcaac cccgccgggg tcgtccccaa cgccgatggc
     3961 ctctccgagg ccctcatgca gcagatcgcg cgcgagctca acaactccga gaccgccttc
     4021 agcatcaaca gcggcggcga gggctacgac gtcgaggtcc gcttcttcac cccgacgcgc
     4081 gaggtcccga tctgcggcca cgccaccgtc gccacccact acgtctacgc gagggagcgc
     4141 ggcatccgga gcggcatcat caggcagaag accaaggcgg gcatccttcc ggtggagatc
     4201 cttcccgagg acgacgacct ccgcgacgtc atgacccagg ccgggatcga gtacggcgcc
     4261 atcctcgggg gcgaggagcg cgaggcactc ctccagggcc tgggcatcgg ag
//
LOCUS       DASSQV010000375         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_959469, whole genome shotgun sequence.
ACCESSION   DASSQV010000375 DASSQV010000000
VERSION     DASSQV010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959469"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFL04_06565"
     CDS             complement(<1..574)
                     /locus_tag="VFL04_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7839405.1"
                     /translation="MRKIVGLALVGLIAAAALGAQTINVLLWDDPYPKALQAMLPEFE
                     KATGIKVNIEMLQPPQVLTKTSVAVGKTSSDYDLVAMDEGNVPLFANLMVPYEAWPAG
                     KKYPKVGKETVTPAMLDVGMWDGKLIGLPINGNLYVWMTRKDLVENPQYRKDFKAKYN
                     YELGVPKTFKQMLEMGSFFNERGIAAGFGPF"
     gene            814..1596
                     /locus_tag="VFL04_06570"
     CDS             814..1596
                     /locus_tag="VFL04_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET7839406.1"
                     /translation="MRGSGRIIAQRRQKLIQLLEREGGSKVLELRERLGVSEITIRRD
                     LEELADEGLVERYHGGARLKDRPVMELLYEDKSLLHAATKNAIGAAAAALVSERSTIL
                     LNAGSTALAVLHHLRDRSLRVVTNNAAAPAEIGGSNVELILLGGEYRAKSRSLFGDFA
                     ALTLSQVHASMCILGVNGVSAKTGLTFSVYTEAAINRLMAERCNGEVVVVADGTKVGT
                     TSNFACVPISRVHVLVTDASADPQELAAIEASGVRVIVVQTA"
     gene            complement(1683..>2350)
                     /locus_tag="VFL04_06575"
     CDS             complement(1683..>2350)
                     /locus_tag="VFL04_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7839407.1"
                     /translation="EGGKAVEDTVKAMKEIASSIGIIEEIARQTNLLALNAAIEAARA
                     GDAGKGFAVVASEVRKLAERSQKAAGEISVLSRDSVEVAERAGDLLRRIVPDIQKTSD
                     LMQEIASSSREQSSGVEQVTGAIGQLDSVIQQNSASSEELASSAEELSGQAVALQDTM
                     AFFKLGGEGEAPVQRAAPKAVPARAQVPSPAKPAPRVTARPSSGTGIVPLPDDKSSEF
                     EAF"
BASE COUNT          378 a          803 c          757 g          412 t
ORIGIN      
        1 tgaagggtcc gaagcccgcg gctatgcccc gctcgttgaa gaagctaccc atctcgagca
       61 tctgcttgaa ggtcttgggc acgccaagct cgtagttgta cttggccttg aagtccttcc
      121 ggtactgcgg attctcgacg aggtccttgc gggtcatcca gacgtagagg ttcccgttga
      181 tggggaggcc gatgagcttg ccgtcccaca tgccgacgtc gagcatggcc ggggtcacgg
      241 tctccttgcc gaccttgggg tacttcttgc cggccggcca ggcctcgtag gggaccatga
      301 ggttcgcgaa caggggcacg ttgccctcat ccatcgcgac gaggtcgtag tccgacgagg
      361 tcttgcccac agcgaccgag gtcttggtga ggacctgggg aggctggagc atctcgatgt
      421 tgaccttgat gccggtggcc ttctcgaact cggggagcat ggcctggagg gccttcggat
      481 aggggtcatc ccagagcagg acattgatcg tctgcgctcc gagcgcggcg gcggcgatca
      541 gcccgacgag ggccaggccc acgatcttcc tcatgatagc ctcctttggc ttttctttct
      601 gcgtgaaagt aaaccacgcc ctcatacggc tgtcaaccta tttttcgtcc cggaagacgg
      661 cccgtccagt gagaatcgag gatatagcct tgcgcatgcc gttgggaggc gataagatac
      721 gatcaataaa caggctatct gatcggaagc ttccggctac atcgacgcgc tgcgcgcatc
      781 ttcatcttta cccgatcggc ggaggacgca ggattgaggg gctccggacg gatcatcgct
      841 caaagaagac agaaactcat ccagctcctc gagcgcgagg gcggctcgaa ggtgctcgag
      901 ctccgcgaga ggctgggcgt ctccgagatc accatcagac gcgacctcga ggagctcgcc
      961 gacgagggcc tggtcgagcg ctaccacggg ggggccaggc tcaaggaccg ccccgtcatg
     1021 gagctcctgt acgaggacaa gagcctcctc cacgcggcca ccaagaacgc catcggcgcg
     1081 gcggccgcgg ccctggtgag cgagcgctcc acgatcctgc tcaacgccgg ctcgaccgcc
     1141 ctcgccgtcc tccaccacct gcgcgaccgc tccctgcgcg tggtgactaa caacgcggcc
     1201 gcccccgccg agatcggcgg ctccaacgtc gagctcatcc tcctcggcgg ggagtacagg
     1261 gccaaatccc gctccctctt tggggacttc gccgccctga ccctctccca ggtccacgcc
     1321 tcgatgtgca tcctcggcgt caacggggtg agcgccaaga ccggcctcac cttctcggtc
     1381 tacaccgagg cggcgatcaa ccgactcatg gccgagcgct gcaacggcga ggtcgtcgtc
     1441 gtggccgacg gcaccaaggt cgggacgacc tcgaacttcg cctgcgtgcc catctcgcgg
     1501 gtccacgtcc tagtcaccga cgcctcggcc gacccccagg agctcgccgc catcgaggcc
     1561 tcgggggtga gggtcatcgt cgtgcagacg gcctgagggc gcacgcaaaa actgaggctc
     1621 ctcgggctcc gccatgatcg gcgggccggg gagcctcggg tccttggccg agcccagtcc
     1681 ccctagaagg cctcgaactc cgagctcttg tcgtcgggga gggggacgat gcccgtccct
     1741 gaggagggcc tcgccgtgac cctcggcgcc ggcttcgcgg ggctcggcac ctgggcccga
     1801 gccgggacgg ccttgggagc ggccctctgg acgggggcct cgccctcgcc gccgagcttg
     1861 aagaaggcca tcgtgtcctg gagggccacc gcctgccccg acagctcctc ggccgatgag
     1921 gccagctcct cggatgaggc cgagttctgc tggatcaccg aatcgagctg gccgatcgcc
     1981 cccgtgacct gctccacccc cgagctctgc tcccggctgg atgaggcgat ctcctgcatg
     2041 aggtcggaag tcttctggat gtcggggacg atcctgcgaa gcaggtcccc ggcgcgctcg
     2101 gccacctcaa cggagtcccg cgagagcacg gagatctcgc cggcggcctt ctgggaccgc
     2161 tcggcgagct tgcgcacctc gctcgccacc acggcgaagc ccttgccggc gtcgccggcc
     2221 ctggcggcct cgatggcagc gttgagcgcc agaaggttgg tctgccgggc tatctcctcg
     2281 atgatgccga tgctcgaggc gatctccttc atggccttca cggtgtcctc gacggccttg
     2341 cctccctcga
//
LOCUS       DASSQV010000376         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1704168, whole genome shotgun sequence.
ACCESSION   DASSQV010000376 DASSQV010000000
VERSION     DASSQV010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704168"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFL04_06580"
     CDS             complement(<1..342)
                     /locus_tag="VFL04_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839408.1"
                     /translation="MLHKLIDVLMNSFRALGSPIRPAEIERIAIMVHRVMSYQSRQFH
                     TLEHVFGFMEGADAEMVLAAVFHDLIYFQVDEGLPKGVAELVLPYMDVEDTSVRLKAD
                     IPQSDVCYHTLR"
     gene            527..832
                     /locus_tag="VFL04_06585"
     CDS             527..832
                     /locus_tag="VFL04_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839409.1"
                     /translation="MRWAWLVLALVLVLVSLSGCAPGPNELQGPTDSRGRLPGFWQGL
                     WNGAIAPVTFIVSLFTDRVQPYEVHNSGRWYNFGYILGLSIAFGGGAGGAAARRRRA"
     gene            922..>2817
                     /locus_tag="VFL04_06590"
     CDS             922..>2817
                     /locus_tag="VFL04_06590"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET7839410.1"
                     /translation="MKKTGRRVAGAFGLSLAAAALLAGLALALLPKSPPEILEYRDVS
                     GTRKPLLVAKDYAVVAGTPWAAQAARETLERGGNACDAAVAALLALNVTHGEAASFPG
                     VAPTMYYNAARGEAKSYIGAGVAPAAATIEAFRKRGFKTVPEMDIWSQLVPASPDVVF
                     ALLKEGGTMSFGELAAPAIALARGGFPAHEVILENFSLSLLKRIGLSILLPYNSKVWL
                     HGQWWRPMALHERLSFPELADSLASIAQAERKVLEAGGTREEGLKAARDYFYKGPIAE
                     RIAAFHRAKGGLMSLADLASYSGAWESPVSGSYGGYTIESNGAWSQGMVAPLALQILE
                     GIDLKALGHNSPAYIHVVTQALELAMVDRDAYMGDPARVKVPVQALLSKAYAAQRRAA
                     MTTRAFKPLPSPGRVEAPGARLLPAPSGEPEGDRFAGVGAIASLMYGPRARLGQDTSQ
                     IVVVDRAGNAVVMTPSDFPKTPMVPGTGLTLGNRMNQFRLDPASPNALEPGKRPRITP
                     HAALVFRGGKLVLAFSTPGGDMQAQALVQVFLDMFVFGMDVESAVSAPRFYSVGVPSS
                     FAPHESFPGTVRLEAQLHEKAAGGLAALGYTVLRDPDWDKDYGAVGAIMVGADGRLYA
                     GSDPREET"
BASE COUNT          450 a          945 c          966 g          456 t
ORIGIN      
        1 cctgagggtg tggtagcaga catcggattg cggtatgtcg gccttgaggc ggacgctcgt
       61 gtcctcgacg tccatgtagg gcaggaccag ttcggccact cccttcggca ggccctcgtc
      121 gacctggaaa tagatcaggt cgtggaagac ggcggccagg accatctcgg cgtcagcccc
      181 ctccatgaag ccgaagacgt gctcgagggt gtggaactgg cgcgactggt aggacattac
      241 gcggtggacc atgatcgcga tccgctcgat ctccgcgggg cggattggag acccgagggc
      301 gcggaaggag ttcatgagga catcgatcaa cttgtgaagc atgccttggc tcatggcctt
      361 agtctcctat ctaagaaaat atcgcgcatg gagggcagga cagcaaatgg gccgaaagtc
      421 aggggcgcgc cccggcgctc gattgcgggc tccgggcccc gatgctacca tgcggaaacc
      481 gccgccggcc ttgaggcttg acttggtgcg gtctggaggt gatgacatga ggtgggcgtg
      541 gctggtcctg gcgcttgtcc tggtcttggt ttccctttcc ggttgcgctc cgggcccgaa
      601 cgagctccag gggccgaccg actcgcgggg caggctgccg ggcttctggc aggggctctg
      661 gaacggggcg atagccccgg tcaccttcat cgtctccctg ttcaccgacc gcgtgcagcc
      721 ctacgaggtc cacaacagcg gccgctggta caatttcggc tatatccttg ggctcagcat
      781 tgccttcggc ggcggggcgg gaggggccgc ggcgcgacgc aggagggcct aggatcgggg
      841 cccgcctctc cttcggcaac ccgcgcttgt ggcccgaggg gacaggggct agactggccc
      901 gcgcacagcg aagggggcgt catgaagaag acaggaagaa gagtcgcagg ggccttcggc
      961 ctctcgctcg cggccgccgc gctcttggcg ggtctcgctt tggccctcct gcccaagagc
     1021 cctccggaga tcctcgagta ccgcgacgtc agcggcacga ggaagcccct ccttgtggcc
     1081 aaggactatg ccgtggtcgc ggggacgccc tgggcggccc aagcggcgcg cgagaccctc
     1141 gagcggggcg gcaatgcctg cgacgccgcg gtcgccgccc tcctcgccct caacgtcacg
     1201 cacggagagg ccgcatcctt cccgggtgtc gcaccgacga tgtactacaa cgcagccaga
     1261 ggcgaggcga aaagctacat tggggccggc gtcgccccgg cggcggccac catcgaggcc
     1321 ttcaggaaac gcggtttcaa gacggtcccc gagatggaca tctggtccca gctcgtcccc
     1381 gcatcccccg acgtggtctt cgccctcctc aaggaaggcg gcacgatgtc cttcggcgag
     1441 cttgcggccc cggccatagc cctcgcccga ggaggtttcc ccgcacatga ggtgatcctc
     1501 gagaacttct cgctgtcact gctcaaaagg atcgggctgt ccatcctcct gccctataac
     1561 tcgaaggtct ggctgcacgg gcagtggtgg cggcccatgg ccctgcacga gcgcctcagc
     1621 ttccccgagc tcgccgacag cctggcctcg atcgcccagg cggagaggaa ggtccttgag
     1681 gccgggggca ccagggagga gggcctgaag gcggcgaggg actatttcta caaggggccc
     1741 atcgccgaga ggatagccgc cttccacagg gccaagggcg ggctcatgag cctcgccgac
     1801 ctcgcctcgt actcgggggc atgggagagc cccgtctcgg gaagctatgg cggctacacc
     1861 atcgagtcca acggggcctg gtcccagggc atggtggcgc ccctcgccct ccagatcctc
     1921 gaggggatcg acctcaaggc actggggcac aactctccgg cttacatcca cgtggtgacc
     1981 caggccctgg agctggcgat ggtcgacagg gacgcctata tgggggaccc tgccagggtg
     2041 aaggtgccgg tgcaggccct gctctcgaag gcctacgctg cccagcggcg ggccgccatg
     2101 accacccggg ccttcaagcc cctgccctcg ccggggaggg tcgaggcccc gggagccagg
     2161 ctcctgcctg cgccgagcgg ggagcccgag ggggacaggt tcgccggcgt cggcgccatc
     2221 gcctctctga tgtatgggcc gagggccagg ctcggccagg acacctccca gatcgtcgtg
     2281 gtcgaccgcg ccgggaatgc cgtggtgatg accccctcgg atttcccgaa gacccccatg
     2341 gtgcccggaa ccggacttac cctcggcaac aggatgaacc agttccgcct cgaccccgcc
     2401 agtcccaacg ccctcgagcc cggcaagcgg ccgcggataa ccccgcatgc ggcgcttgtc
     2461 ttccgcggag gaaagctggt gctggccttc agcacccccg gtggggatat gcaggcccag
     2521 gccctcgtgc aggtcttcct ggacatgttc gtgttcggta tggacgtgga gtcggcggtg
     2581 agcgcccccc gtttctattc ggtgggcgtc ccctcatcct tcgcgcccca cgagtccttc
     2641 cccggcacgg tccgcctgga ggcccagctc cacgagaagg cggctggggg cctcgccgcc
     2701 ctcggataca ccgtgctgcg cgaccccgac tgggacaagg actatggcgc ggtgggcgcg
     2761 atcatggtgg gcgccgacgg caggctctac gccggctccg atccgcggga ggagacc
//
LOCUS       DASSQV010000377         4862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2452300, whole genome shotgun sequence.
ACCESSION   DASSQV010000377 DASSQV010000000
VERSION     DASSQV010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2452300"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..704)
                     /locus_tag="VFL04_06595"
     CDS             complement(33..704)
                     /locus_tag="VFL04_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839411.1"
                     /translation="MRKFSLALVIAVLTASSLLSAQTPTAVPAERMKWPSAYVHPDMP
                     VYGAGEFKGWNRWAANDDYGIFMLIDKTGQADLDSYAAKLKAAGFEQKGSKEFRKGIF
                     RLAFQFNSKTILQISSYRDKVEAWPSALISGVPELKRGYLTGIILPSEDMPGYLQLYF
                     IGLSQGELDAWFAQLAAAGFKVQDGSATKPGMALQGKKWKLLSIQAGENGPGEWMLDF
                     SYADE"
     gene            829..1692
                     /locus_tag="VFL04_06600"
     CDS             829..1692
                     /locus_tag="VFL04_06600"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7839412.1"
                     /translation="MFGKIFLEITSHCNLSCSFCPPTRRPRSQLSPELFDRLLARLEG
                     QGKKLYFHLKGEPLIHPSLGRFLETAGQHGFSVSLTTNGTLVQERADELLAATNLEKL
                     SISLHSHVGEAGLEAYWARVSAFLDRHRAAPRFPVSLRLWSRGSGGLPPETERLWTLV
                     RERYPAAGGWEDSSTSYKSREMDRLVYLNQADRFEWPDPELPQDGPRGFCRGLGNQVG
                     VLADGTVVPCCMDGEGRIALGNLATSSLEEILASPRARAIRAGFARRELVEPLCRTCG
                     YRRRFDRRGLA"
     gene            1805..2683
                     /locus_tag="VFL04_06605"
     CDS             1805..2683
                     /locus_tag="VFL04_06605"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7839413.1"
                     /translation="MKKISPFLWFDKEAGEAAELYVSTFGASRIVSRSTMGDTPSGAV
                     EIIVAELAGQTFKLMSAGPYFKFTPAISFTVACSSKAEVDAAWARLSEGGSTFLELGS
                     YTFSERYGWTSDRYGLSWQLMYRPQSQARQKITPTLMFVGANCGKAEEAISHYFSFFG
                     GSGLGDVMRYGEGEEPNKAGTLRHVSFSLAGQGFAAMDSAQDHRFTFSEAISFEVECA
                     DQAEVDHYWRMSADPSAEQCGWLKDRYGVSWQIVPAALQRMMGDSDPARVQRVSKAFL
                     AMKKFDIAALEAAYAG"
     gene            2857..3750
                     /locus_tag="VFL04_06610"
     CDS             2857..3750
                     /locus_tag="VFL04_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839414.1"
                     /translation="MEPGERKRPKVVLAKKDGAVLKYEAYLKARPEARCRKDGPGQGL
                     RKIMELQAGLPPPPGVEADTGAIQLYGWPFDGHGVLGLLTEEVGYEMGQTRVFESYEP
                     FPLENSPSEPEVWHGTVEDGLAIQLEVQRDFRPVAGTHGQPTGKFRVSPALYYGGIAD
                     FHDLGIVTIRAETSVQLLDERFVDEQGGVTDPAGVLGLWSSWGPEPFSSTMTYINSGV
                     MPIPGREVQCQASSENGAVPGTVVRVTQTFSIQTSGAVACFDYLGAIVTRTPTLSLWH
                     MRPLRRVFPILPIPHGHFFHI"
     gene            complement(3848..>4862)
                     /locus_tag="VFL04_06615"
     CDS             complement(3848..>4862)
                     /locus_tag="VFL04_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839415.1"
                     /translation="SWRARADVLFYDFVLQTEAGETVLERELTEPELRLNLGPGEYRY
                     RIVPHNLLGKPEGDQVWRRLRVLKAEAPRVTGLSGKTWFLEDLEPRLRLSGDELMPGA
                     RVELIPPSSARPIEGRETGRQANSELSVDFPAEGLSVGEYALMVTNPGGLSFRLPGAI
                     LVRYQRPVELSLSFGLAPWLALYDSAYKEAWPGTFFPLGSASRLDFYFFRRPYGQFGI
                     EVALGGRLMEGGSPTATIVSQIGLVGGNCLYKYSFSRLLSMAARAGVGLSLSHHAFDY
                     SGSKGQELSSIDPYLSAGLSLQCSLSKRYFLEAGADWMQISAAGITAGGLMPFLSAGM
                     LLF"
BASE COUNT          819 a         1621 c         1615 g          807 t
ORIGIN      
        1 gagctcgtcg gtcatgcgcg gcctcgcctt tcctattcgt cggcgtagct gaagtcgagc
       61 atccattccc ctggtccgtt ctccccggcc tggatgctca ggagcttcca ctttttgccc
      121 tgcagcgcca tcccaggctt cgtggccgat ccgtcctgga ccttgaagcc cgcggcggcg
      181 agctgggcga accaggcgtc gagctcccct tgggagaggc cgatgaagta gagctggaga
      241 tagccgggca tgtcctccga gggcaggatg atgcccgtga ggtagcccct cttcagctcg
      301 ggcacaccgg aaatcagggc cgagggccag gcctcgacct tgtcccggta ggaggagatc
      361 tgcaggatgg tcttcgagtt gaactggaag gcgagtctga agatgccctt gcggaactcc
      421 ttgctgccct tctgctcaaa gccggcggcc ttcagcttgg ccgcatagga gtccaggtcg
      481 gcctggcccg tcttgtcgat cagcatgaag atgccgtaat cgtcgttcgc ggcccagcgg
      541 ttccagccct tgaactcccc ggcgccgtag accggcatgt cgggatggac gtaggccgag
      601 ggccacttca tgcgctcggc gggcacggct gtcggggtct gggcactgag gagggaggag
      661 gcggtcaaga cggcgatgac aagggctagg ctgaactttc tcaattgggt ctccatgggg
      721 gaaatttcgg cgagtatagc ctgggtcctt ggccaggacc agtgagggtt tcccagagga
      781 cggaccgccg cctaggagac ccctgcccgc ccatgctaca agtgctcgat gttcggcaag
      841 atattcctcg agatcacgag ccactgcaac ctgtcctgct ccttctgccc gcccacgcgc
      901 cggccccgaa gccagctctc ccccgagctc ttcgaccgtc tcctggccag actcgagggg
      961 caggggaaaa agctctattt ccacctcaag ggcgagcccc tcatccaccc ctccctcggg
     1021 cgcttcctcg agacggcggg gcaacacggc ttctcggtct cgctcaccac caacggcacc
     1081 ctggtccagg agagggccga cgagctccta gccgccacca acctcgaaaa gctgagcata
     1141 tcgctccaca gccatgtggg tgaggccggg cttgaggcct actgggctcg ggtttcagcc
     1201 ttcctggaca ggcaccgcgc agcgccccgt ttcccggtga gcctccggct ctggagccgc
     1261 ggctcagggg gccttccgcc cgagaccgag cggctctgga ccctcgtgcg cgagcgctat
     1321 cccgccgcag gcggctggga ggactcctcg acaagctaca agtcgcgcga gatggacagg
     1381 ctggtctacc tgaaccaggc ggatcgtttc gagtggcccg accccgagct tccccaggac
     1441 gggccgaggg gcttctgccg cgggctcggc aaccaggtgg gggtgctcgc cgacggcacc
     1501 gtggtgccct gctgcatgga cggagagggg cggatcgccc tgggcaacct ggccacctcc
     1561 tcactcgagg agatcctcgc ctcaccgcgg gccagggcga tccgcgcggg tttcgcgcgg
     1621 cgcgagctcg tggagccgct ctgcaggacc tgcggatacc gcaggaggtt cgaccggagg
     1681 ggcctggcct aggcctgcct gcccagcccg cccagcggcg aggctggccg ggcatcggcc
     1741 ccctccctgc cgattctcag tacattacac accatgtctg tgttcgactc ggaaggggca
     1801 agcgatgaag aagatatcgc cgtttctctg gttcgataag gaggcgggcg aggccgccga
     1861 gctctacgtc tccaccttcg gcgcttcgag gatcgtaagc cggtcgacga tgggcgacac
     1921 gccctcgggg gccgtagaga tcatcgtggc cgagctcgcg ggccagacct tcaagctcat
     1981 gtcggcagga ccctacttca agttcacccc agccatatcc ttcaccgtgg cctgctcgtc
     2041 gaaggccgag gtcgacgcgg cctgggcgag gctgtcggag ggcggatcca ccttcttgga
     2101 gctcggctcc tacaccttca gcgaacgcta cggctggacc tcggaccgct acgggctctc
     2161 gtggcagctc atgtacaggc cgcagagcca ggcgcggcag aagatcacgc cgaccctcat
     2221 gttcgtcggc gccaactgcg gcaaggccga ggaggccatc tcccattact tctcattctt
     2281 cgggggatcg gggctcgggg acgtcatgcg ctatggggag ggagaggaac cgaacaaggc
     2341 cggcaccctg aggcacgtgt ccttcagcct ggcgggccag ggcttcgccg ccatggacag
     2401 cgcccaggac caccgcttca ccttcagcga ggcgatctcc ttcgaggtcg agtgcgccga
     2461 tcaggccgag gtggaccact actggaggat gtcggccgac ccgagcgccg agcagtgcgg
     2521 ctggctcaag gacagatacg gggtctcgtg gcagatcgtc cccgctgccc tgcagaggat
     2581 gatgggagac tccgaccccg ccagggtcca gcgcgtctcc aaggccttcc tggccatgaa
     2641 gaagttcgac atagccgcgc tcgaggcggc atacgcgggc taggcccccg tctcttcccc
     2701 ctccgcgccg attgacagcc catcggcgcg cgcccggttg acatccttcc gcaccggatg
     2761 taaaagtttc ttgacatctg tgttccgcct gccatagtcg cgcaatccga aactgctggt
     2821 cgatctgtac gaaatatctc ggggggaggt gtctgtatgg aaccaggaga gaggaagagg
     2881 ccgaaggtgg tgctggcgaa gaaggatggg gcggtgctca agtacgaggc ctacctaaag
     2941 gcccgtcccg aggcccgctg cagaaaggac ggacccggcc agggtctgcg gaagatcatg
     3001 gagcttcagg cgggcctgcc gcctccgccc ggagtggagg ccgacaccgg agccatccag
     3061 ctctatggct ggcccttcga cggccatgga gtcctgggcc tcctgaccga ggaggtcggc
     3121 tacgaaatgg gccagacccg ggtcttcgag agctatgagc cctttccgct ggagaacagc
     3181 ccctccgagc ccgaggtctg gcacgggacc gtcgaggatg gcctcgccat ccagctcgag
     3241 gtgcagaggg atttcaggcc cgtggccggc acccatggcc agcccacggg gaagttccgg
     3301 gtgagcccgg ccctctacta tggcggcatc gcggatttcc atgatctcgg aattgtgacg
     3361 atcagggccg agacctcggt gcagctcctg gacgagcgct tcgtcgacga gcagggcggg
     3421 gttaccgacc cggcgggcgt gctgggcctc tggtcttcgt ggggccccga gcccttttcg
     3481 tcgaccatga cctacatcaa cagcggcgtg atgcccattc ccggacgcga ggtccaatgc
     3541 caggcttcga gcgagaacgg ggcggtcccc ggcacggtgg tccgcgtcac gcagaccttc
     3601 tcgatccaga ccagcggggc cgtggcctgc ttcgactacc tcggagccat cgtcacgcgc
     3661 acgcccaccc tgagcctctg gcacatgagg ccgctccggc gggtcttccc gatcctcccc
     3721 atcccgcacg ggcacttctt ccacatctga gcctggcagg acgccggcgc ccccccccgc
     3781 agaggggcgc cacggcctcc gcccggccgg cgccgccatg gatgacgggc gcctcggccc
     3841 gctgggtcta aaacaggagc atgcccgccg agaggaaggg catgaggccg cccgcggtga
     3901 tgcccgcggc cgagatctgc atccagtcgg ccccggcctc caggaagtag cgcttcgaga
     3961 gcgagcactg cagggacagg ccggccgaca gatagggatc gatgctcgag agctcctgcc
     4021 ctttgctgcc cgaatagtcg aaggcgtggt ggctcaggga gagcccgacc ccggccctgg
     4081 cggccatcga gagcagcctg gagaaggaat atttgtagag gcagttccct cccacgagcc
     4141 ctatctgcga cacgatggtg gccgtcgggc tgcccccctc catgagccgg ccccccagcg
     4201 cgacctcgat cccgaactgg ccatagggtc gtctgaagaa gtagaagtcc agcctgctcg
     4261 ccgagccgag ggggaagaag gtcccgggcc aggcttcctt gtaggccgaa tcgtagaggg
     4321 ccagccaggg ggcgaggccg aaggaaaggg agagctccac cggcctctgg tagcgcacga
     4381 ggatggcgcc gggaagcctg aaggacaggc ccccggggtt ggtgaccatg agggcgtatt
     4441 cgcccacgga cagaccctcg gcggggaaat cgacgctcag ctccgagttg gcctgtctcc
     4501 ccgtctccct tccctcgatg ggcctcgcgg acgagggagg gatcagctcg acccgggccc
     4561 cgggcatgag ctcgtcccct gacagcctga gcctgggctc aaggtcctcg aggaaccagg
     4621 tcttgccgga gaggcccgtg acccggggcg cctcggcctt gaggacccgg agcctccgcc
     4681 agacctggtc gccctcgggc ttgcccagga ggttgtgcgg cacgatcctg tagcggtatt
     4741 ccccagggcc aaggttgagc ctcagttcgg gctccgtgag ctccctctcg aggacggttt
     4801 cgcccgcctc ggtctggagc acaaaatcgt agaacaggac gtcggctctg gcccgccagc
     4861 tg
//
LOCUS       DASSQV010000378         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1081769, whole genome shotgun sequence.
ACCESSION   DASSQV010000378 DASSQV010000000
VERSION     DASSQV010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081769"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFL04_06620"
     CDS             <1..801
                     /locus_tag="VFL04_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HET7839416.1"
                     /translation="LARCEPERIEDDQRFAGRILFPWNDRIPGGSYSFAGERHSLRIN
                     CAEDGSAIHGFLYRRTMELVGDYADADSARLVLRARLLPGEEPGYPFAPSLRVEYRLE
                     EGRFSLGFTMRNDGQAPAPVALGWHPYFRLEGPSENWLFEHKGESFVPVGPDLMPLGA
                     PEPVAGSPWDFRRARPLGKGPIDIALAASADGLCRLRGPGGLELRIEADPRLFRYTQL
                     YLPPERDALAIESVSAATDAFNRDGLGLIVLAKGEERSGSVSLRLGRA"
     gene            complement(798..>2208)
                     /locus_tag="VFL04_06625"
     CDS             complement(798..>2208)
                     /locus_tag="VFL04_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839417.1"
                     /translation="LGPDSSLREALGKAMSDSASARTALRGSASEFAYLFQASTLLLN
                     RRVERAWHSFEEEAAIQRASLGEYYDALGLDAKAARQYRRALAVDPSNVQVMYSLARA
                     EERGGNWPYAISLLGRVAAADPSHPEAASRHNALARAHAARMDGEASFSSDTNSLAYR
                     ATSLLSLPVGARLSLEPSLQVSSVRDWNQGVPAYLSAEFGLALPFSISSGALELRPRA
                     SFYGTSSNYAAQGSSTLSPQDFAASLRLMTGAGASLSWRSRLVSARGDYAWAPLPDTV
                     DPIFTPLYAHRLELSASAYLPMTGFMRYLAPRAYGLGYYIPADGNLLGTGLLELVPAA
                     RLSDSPWASLGLPVCVQLEDSSRPLSSPYYAADAALTAKTGLRLQTVFGLRRGDRVSI
                     MVQALGGLYETGAFSAAPDRSGYAQLVARLELGIGDASYFVSIEATQTAKGLMSRPGY
                     WSLSVLGGLSAKQSRLVAP"
BASE COUNT          323 a          743 c          818 g          324 t
ORIGIN      
        1 ctcgcgcgct gcgagcccga gcgcatcgag gacgatcagc gtttcgcggg caggatcctc
       61 tttccctgga acgaccgcat ccccggcggc tcctacagct tcgccggcga gaggcactcc
      121 ctcaggatca actgcgccga ggacggctcg gccatccacg gtttcctcta ccggcgcacg
      181 atggagctgg tcggggacta cgccgacgcg gactcggcca ggctcgtcct gcgcgcgagg
      241 ctcctgcccg gcgaggagcc cggctatccc ttcgccccct cgctcagggt cgagtacagg
      301 ctcgaggagg ggcgcttctc cttgggcttc accatgagga acgacggcca ggccccagcc
      361 cccgtggccc ttggctggca tccctatttc aggctcgagg ggccgagcga gaactggctg
      421 ttcgagcaca agggagagtc cttcgtcccc gtcgggcccg acctcatgcc ccttggggcc
      481 cccgagcccg tggccgggag cccctgggat ttccgccggg ccaggcccct cgggaagggc
      541 cccatcgaca tcgcgttggc cgcctccgcc gatgggctct gccgcctcag gggaccgggt
      601 ggcctcgagc tcaggatcga ggccgaccct aggctcttcc gctacaccca gctctacctg
      661 ccgcccgagc gcgacgccct cgccatcgag agcgtgagcg cggccaccga cgccttcaac
      721 cgcgatggcc tcggcctcat cgtcctcgcg aagggcgagg agcgctcggg ctcggtctcg
      781 ctccgcctgg gcagggccta gggcgccacc agacggctct gcttcgccga caggcccccg
      841 aggaccgaga gcgaccagta gccgggcctc gacatgagtc ccttggcggt ctgggtggcc
      901 tcgatgctga cgaagtagct tgcgtccccg atgccgagct cgagcctcgc gacgagttgg
      961 gcgtagccgc tgcggtcggg ggcggcggag aaggcccccg tctcgtagag gccgcccagg
     1021 gcctgcacca tgatcgagac cctgtccccg cgtctgagcc caaagaccgt ctggagcctc
     1081 agccccgtct tcgccgtgag cgctgcgtcg gccgcgtagt aggggctcga gagcggcctc
     1141 gaggagtcct cgagctggac gcagacggga aggccgaggc tagcccaggg cgagtccgag
     1201 agccgggcgg cggggacgag ctcgaggagg cccgtgccga ggaggttccc gtcggcgggg
     1261 atgtagtagc cgaggccata ggccctcggg gccaggtagc gcatgaagcc cgtcatcggc
     1321 aggtaagccg aggccgagag ctcgagcctg tgggcgtaaa ggggcgtgaa gatcggatcg
     1381 acggtgtcgg gcaggggggc ccatgcgtag tcgccgcgcg ccgagacgag acgcgagcgc
     1441 cacgagagcg atgccccggc tccggtcatg agcctgaggc tcgcggcgaa gtcctggggc
     1501 gagagggtcg agctcccctg ggcggcgtag ttgctgctcg tgccgtagaa gctcgccctg
     1561 ggcctgagct caagggcccc cgaggagatc gagaagggca gggccaggcc gaactcggcc
     1621 gagagatagg ccggcacgcc ctggttccag tcgcggaccg atgagacctg gagggagggc
     1681 tcgaggctga gccgggcgcc caccgggagg gagaggaggc tcgtcgccct gtaggcgagg
     1741 ctgttcgtgt ctgagctgaa tgaggcctcg ccgtccatgc gcgcggcgtg ggcccgggcg
     1801 agggcgttgt gccggcttgc cgcttcggga tggctcgggt cggccgcggc caccctgccg
     1861 aggagggaga tggcataggg ccagttgcct ccgcgttcct cggccctggc caggctgtac
     1921 atcacctgga cgttgctcgg gtcgacggcg agggctcggc ggtattgcct tgccgccttc
     1981 gcgtcgaggc cgagcgcgtc gtagtattcg ccgaggctgg ccctctggat ggccgcctcc
     2041 tcctcgaagg aatgccaggc ccgctcgact ctccggttca ggaggagggt ggaagcctgg
     2101 aagagatagg cgaactcgga ggccgatccg cgcagggcag tgcgggccga ggcgctgtcc
     2161 gacatggcct tcccaagggc ctcgcggagg gaggagtcgg gccccagc
//
LOCUS       DASSQV010000379         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1581531, whole genome shotgun sequence.
ACCESSION   DASSQV010000379 DASSQV010000000
VERSION     DASSQV010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581531"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFL04_06630"
     CDS             complement(<1..418)
                     /locus_tag="VFL04_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HET7839418.1"
                     /translation="MDYGYFDDQRREYVITRPDTPLPWLNYLGQDDFFGLCTNTAGGY
                     SFWRDSRLRRLTRYRYNSMPYDSGGRYLYVNDSGSVWNPGWKPVKAALDAYECRQGLG
                     YTRILGAKDGLEVELLFFVPPKCDHEIWRVRVRNRSR"
     gene            547..>2285
                     /locus_tag="VFL04_06635"
     CDS             547..>2285
                     /locus_tag="VFL04_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7839419.1"
                     /translation="MDDAAISVEELLAREPNPTARAVGAVEWLSANFRKNPHWAKSLA
                     ERELAHAIAGGATSSVAWIRFYIGWIHIDEDDYEAALKVLDEARADFEQCAEPAGTAR
                     TFNAIGAAYLGLGIYDLALDYFERCTECAEALDRPDMAGAALINTADCLYELERYGEA
                     LEVIERSFEYKVFAHNESVRHQVAGMIYRALGRREEAEEQLLSGLAKASGVDFQSVES
                     IRALAELYLDQARFQEAQDLIERGLEVADRVNDRLHGAQLRLVRAKTDRALGRKDRAL
                     EDIRAAIATTTEVASRKGEADAYKAYSEALRDEGDFEASLEALLHHYELKDSIRTEQL
                     DRRIVGIKKEQARREEVLFRERLEQLSILGEIGRRITSSLDLVGIAEAVRSGAARLAE
                     APILLLGTGTNRKMGQFVVVEEGKPAGSVHGGDSALEAIAARCLSSGLEELEERAEPE
                     GSVLGLPLAVGDRVVGALCVRCSRIGAYDKARLEGLRAVAAYAAIALENHRLFRQVEE
                     LASIDPLTGLLNRRSLLACLEEEYRQRRRYGRDVAVVIIDLDRFKSVNDSYGHEAGDL
                     VLVSFADILKRVSR"
BASE COUNT          395 a          775 c          763 g          352 t
ORIGIN      
        1 cccgcgatcg gttcctgacc ctgaccctcc agatctcgtg gtcgcatttc ggggggacga
       61 agaagaggag ctcgacctcg agcccgtcct tggcgccgag gatcctcgtg tagccgaggc
      121 cctgtctgca ctcgtaggcg tcgagggccg ccttcaccgg cttccacccg gggttccaca
      181 cggagcctga gtcgttgacg tagagatagc gaccgcctga gtcatagggc atgctgttgt
      241 agcggtagcg ggtcaggcgc cggagccgcg aatccctcca gaagctgtag ccccccgccg
      301 tgttcgtgca gaggccaaag aaatcgtcct ggccgaggta gttgagccag ggcagcggcg
      361 tgtccggcct ggttatgaca tattcccgcc tctggtcgtc gaagtagccg tagtccatcc
      421 ggtcccccgg aatcagactc tatcggggtt ggttggatgg gtcaagcaac cgacgaaggg
      481 ggagctgggg catcgaagcc agggcccttc atttgtgttt ctccatgcgc ggcgtataat
      541 ggccggatgg acgatgccgc aatttcggtc gaagagctcc tcgcgaggga gccgaacccg
      601 acggcgcgcg ctgtgggggc ggtcgagtgg ctctcggcga acttccgcaa aaacccccat
      661 tgggcgaagt ccctggccga gcgcgagctg gcccatgcca tcgcaggcgg ggccacgagc
      721 tcggtcgcat ggatcaggtt ctacatcggc tggatccaca tcgacgagga cgactatgag
      781 gccgcgctca aggtcctcga cgaggccagg gccgatttcg agcagtgcgc cgagccggcc
      841 gggaccgcac ggaccttcaa cgccatcggc gcggcctacc tcggcctcgg gatctacgac
      901 ctcgccctcg actacttcga gcgctgcacc gagtgcgccg aggccctcga ccggccagac
      961 atggcagggg ccgccctgat aaacaccgcc gactgcctgt acgaactcga gcgctacggg
     1021 gaggccctgg aggtcatcga gcgctcattc gagtacaagg tcttcgccca caacgagtcg
     1081 gtccggcacc aggtggccgg catgatatac cgcgccctgg gcagaaggga ggaggccgag
     1141 gagcagctcc tctccggcct cgcgaaggcc agcggagtcg attttcagag cgtcgagagc
     1201 attcgggccc tcgccgagct ctatctcgac caggccagat tccaggaggc ccaggacctc
     1261 atcgagcggg gcctcgaggt cgccgaccgc gtgaacgacc gcctccacgg agcccagctc
     1321 cgcctcgtgc gggccaagac cgaccgcgcc ctcggcagga aggaccgtgc cctcgaggac
     1381 atcagggccg ccatcgccac caccacagag gtggcctcgc gcaagggcga ggccgacgcc
     1441 tacaaggcct attccgaggc cctcagggac gagggcgact tcgaggcttc gctcgaggcc
     1501 ctcctccacc actatgagct caaggactcg atcaggaccg agcagctcga caggcggatc
     1561 gtcggcatca agaaggagca ggcgcggcgc gaggaggtcc tcttccgcga aaggctcgag
     1621 cagctgtcga tcctcgggga gatcggccgc aggataacct cgagcctcga ccttgtcggc
     1681 atagccgagg ccgtgcggtc gggagcggcc cggctcgccg aagccccgat cctccttctc
     1741 ggcacgggga ccaaccgcaa gatgggccag ttcgtcgtcg tcgaggaggg aaagccagcc
     1801 ggatcggtcc acggcgggga ctcggccctc gaggccatcg cggccaggtg cctcagctcg
     1861 ggcctcgagg agctcgagga gcgcgccgag cccgagggct ccgtccttgg cctccccctc
     1921 gcggtggggg acagggtggt cggcgcgctc tgcgtgcgct gctcccgcat cggggcctac
     1981 gacaaggcca ggctcgaggg cctccgcgcc gtcgccgcct acgccgccat cgccctcgag
     2041 aaccacaggc tcttccgcca ggtcgaggag ctggcgtcca tcgatccact caccggcctc
     2101 ctcaaccggc gcagcctcct ggcctgcctc gaggaggagt acagacagcg ccggcgctac
     2161 ggccgcgacg tggccgtggt gatcattgat ctggaccgct tcaagtccgt caacgacagc
     2221 tacggccacg aggccggtga cctggtcctc gtgagcttcg ccgacatcct caagcgggtc
     2281 tcgcg
//
LOCUS       DASSQV010000380         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1457306, whole genome shotgun sequence.
ACCESSION   DASSQV010000380 DASSQV010000000
VERSION     DASSQV010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457306"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFL04_06640"
     CDS             complement(<1..418)
                     /locus_tag="VFL04_06640"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969751.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET7839420.1"
                     /translation="MNLAPEALIYELSSPGRMGVALPACDVPATPVPAALARTELALP
                     EVDELAVVRHFTRLSQKNYSIDTHFYPLGSCTMKYNPKVNEVAAACPGWRDTHPLVGE
                     QFSQGAIELIYRLQESLREIGGFAAASLQPAAGAHGE"
     gene            complement(415..1734)
                     /gene="gcvPA"
                     /locus_tag="VFL04_06645"
     CDS             complement(415..1734)
                     /gene="gcvPA"
                     /locus_tag="VFL04_06645"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET7839421.1"
                     /translation="MPFIANSDADRRAMLERIGVGSIEELFSDIPAKRRFPSLNIGEP
                     LSELEVLREIEAMAAKNVTASTCAWFLGAGAYNHFIPAVVGAIASRGEFLTAYTPYQP
                     EVSQGTLQAIFEYQSMAAALLGMEVVNASHYDGATALAEAVLMAYNNGGGRHRVLLPA
                     ALHPEYKEVIATYFEAFEVELVEYSGEPSAAGADEKTCCVVASYPDFDGEIRDLKAGA
                     EAAHAAGALFVVHADPVMCALLKSPGELGADIVAAEGQSLGNAMNFGGPFLGMLGVTS
                     KLVRKMPGRIVGQAKDAQGRRGFVLTLSAREQHIRREKAVSNICSNQGLVMLQTCVYL
                     ALMGKKGMRTVAELCWNKAHYAAARIATVPGFAVATKTFFKEFTVKTPVPAEEICTKL
                     TKRCVVPGLPMSRYEAKRSHELLVCVTETCTKAQIDLLVESLKEASK"
     gene            complement(1738..2121)
                     /gene="gcvH"
                     /locus_tag="VFL04_06650"
     CDS             complement(1738..2121)
                     /gene="gcvH"
                     /locus_tag="VFL04_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET7839422.1"
                     /translation="MNIDKNARYRASHEYARKEGAEIVVGISDHAQESLGDIVFVELP
                     EPGKTFKQGEAFGVVESVKAASDLYMPMSGKIVAANGDLQGDPSLVNKDCYGKGWMIR
                     FAPTNPSEWDGLMDAAAYEKEAGKE"
     gene            complement(2135..3373)
                     /gene="gcvT"
                     /locus_tag="VFL04_06655"
     CDS             complement(2135..3373)
                     /gene="gcvT"
                     /locus_tag="VFL04_06655"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET7839423.1"
                     /translation="MPGGPSAEPCPVSLAEPPVSHPLRPLIYHVRLGRTRTSRRRTTL
                     NATRLHDWHVANGAKMAPFAGYDMPISYPTGAVEEHLITRRSAGLFDIDHMGQIEVSG
                     PGADEFVSRTVSSKVIDMKDGDARYSLLLDDKGLVLDDLFVYKLPGRWWIVVNASNRE
                     ADYAWFKALAAKTGNAGLKVIDHSEATYMIAVQGPRAIELMDLVSGGAVSVLPRFTSG
                     QLNVAGIPVLFGRTGYTGEDGGELFFPAEKAVELWELLLATGAKHGIETKPIGLAARD
                     SLRFEAGMPLHGHEISPTINPLEAGFKWACDFEKDFVGKPALEKVQAEGLKRKLVGIE
                     VTGGVPREGYEIVAEGTSTVIGACVAGMFCPTVKKYAANAFLSPESAKVGTKVQVLIH
                     GKPKDAVVIKRPLYIPAYRR"
BASE COUNT          511 a         1036 c         1258 g          579 t
ORIGIN      
        1 gctcgccgtg ggcgcccgcc gcgggctgga ggctagcggc cgcgaagccg ccgatctcgc
       61 ggagggactc ctggaggcgg tagatcagct cgatggcgcc ctggctgaac tgctcgccca
      121 caagggggtg ggtgtcgcgc cagcccgggc aggcggcggc cacctcgttg accttggggt
      181 tgtacttcat cgtgcaggag cccaggggat agaagtgcgt gtcgatcgag tagttcttct
      241 gggagagccg ggtgaagtgg cggaccaccg cgagctcgtc gacctcgggg agggcgagct
      301 cggtccgcgc gagggcggcg ggcaccggcg tggccgggac gtcgcaggcg ggcagggcca
      361 cgcccatcct gccgggcgag gacagctcgt agatgagcgc ttcgggagcg aggttcactt
      421 cgacgcctcc ttcagggatt cgacgaggag gtcgatctgg gcctttgtgc aggtctcggt
      481 gacgcagacc aggagctcgt ggcttcgctt ggcctcgtag cgcgacatgg gcagacccgg
      541 cacgacgcag cgcttggtga gcttcgtgca gatctcctcg gcggggacgg gcgtcttgac
      601 cgtgaactcc ttgaagaagg tcttggtcgc gacggcgaag cccggcaccg tggcgatcct
      661 cgccgcggcg tagtgggcct tgttccagca gagctcggcc acggtccgca tgcccttctt
      721 gcccatgagg gcgaggtaga cgcaggtctg gagcatgacg aggccctggt tcgagcagat
      781 attggagacc gccttctcgc ggcggatgtg ctgctcgcgg gcggacaggg tgaggacgaa
      841 gcccctccgg ccctgggcat ccttcgcctg gccgacgatg cggccgggca tcttgcgcac
      901 cagcttggag gtcacgccga gcatgccgag gaagggcccg ccgaagttca tcgcgttgcc
      961 gaggctctgg ccctcggcgg ccacgatgtc ggctccgagc tcgccggggc tcttgagcag
     1021 ggcgcacatg accggatcgg cgtggaccac gaagagggcg ccggcggcgt gggccgcctc
     1081 ggcgccggcc ttgaggtcac ggatctcgcc gtcgaagtcg gggtaggagg ccacgacgca
     1141 gcaggtcttc tcgtcggccc cggcggccga gggctcgccg gaatactcga cgagctcgac
     1201 ctcgaaggcc tcgaagtagg tcgcgatgac ttccttgtat tcggggtgga gggcggcggg
     1261 caagaggacg cggtgcctgc cgccgccgtt gttgtaggcc atgaggacgg cctcggccag
     1321 ggcggtcgcc ccgtcgtagt gcgaggcgtt gacgacctcc atgccgagca gggcggcggc
     1381 catgctctgg tactcgaaga tcgcctgcag ggtgccctgc gacacctcgg gctggtaggg
     1441 ggtataggcg gtgaggaact ccccgcggga ggcgatggcg cccacgacgg cggggatgaa
     1501 gtggttgtag gccccggccc cgaggaacca ggcgcaggtc gaggccgtga cgttcttcgc
     1561 cgccatcgcc tcgatctcgc gcaggacctc gagctcagag agcggctccc cgatgttcag
     1621 ggaggggaag cgccgcttcg ccggtatgtc ggagaagagc tcctcgatgc tcccgacgcc
     1681 gatgcgctcg agcatggccc ggcggtcggc gtcggagttc gctatgaatg gcacgtatta
     1741 ctccttcccg gcttccttct cgtacgcggc ggcgtccatg aggccgtccc attcggaggg
     1801 gttcgtcggg gcgaaacgga tcatccagcc cttgccgtag cagtccttgt tgacgaggga
     1861 ggggtcgccc tggagatcgc cgttcgcggc gacgatcttg ccggacatgg gcatgtacag
     1921 gtcgctcgcg gccttgaccg actcgacgac gccgaaggcc tcgccctgct tgaaggtctt
     1981 cccgggctcg gggagctcga cgaagacgat gtcgccgagc gactcctggg cgtggtcgga
     2041 gatgccgacc acgatctcgg cgccctcctt gcgggcatat tcgtgcgatg cgcggtaccg
     2101 ggcgttcttg tcgatgttca tggtcgattc ctcctcatct gcgataggcc gggatgtaca
     2161 gcggccgctt tatgacgacg gcgtccttgg gcttgccgtg tatgagaacc tggaccttgg
     2221 tcccgacctt ggcggactcg ggggagagga aggcgttggc ggcgtacttc ttgacagtgg
     2281 ggcagaacat gccggcgacg caggcgccga tgacggtgct cgtgccctcg gcgacgatct
     2341 cgtagccctc gcgcgggacc ccgcccgtga cctcgatgcc gacgagcttg cgcttgaggc
     2401 cctcggcctg gaccttctcg agggccggct tgccgacgaa gtccttctcg aagtcgcagg
     2461 cccacttgaa gcccgcctcg agggggttga tcgtggggct gatctcgtgg ccgtgcagcg
     2521 gcatgcccgc ctcgaaacgg agcgagtcgc gcgcggcgag gccgatgggc ttggtctcga
     2581 tcccgtgctt cgcccccgtg gcgaggagga gctcccagag ctcgacagcc ttctcggcgg
     2641 ggaagaagag ctcgccgccg tcctcgcccg tgtagcccgt ccgtccgaag aggacgggga
     2701 tgccggccac attcagctgg cccgaggtga agcggggcag gaccgagacg gccccgccgg
     2761 agacgaggtc catgagctcg atggccctgg ggccctggac ggcgatcatg taggtggcct
     2821 cggagtggtc gatcaccttg aggccggcgt tcccggtctt ggcggcgagg gccttgaacc
     2881 aggcgtagtc ggcctcgcgg ttcgaggcgt tgacgacgat ccaccagcgg ccggggagct
     2941 tgtagacgaa gaggtcgtcg aggacgaggc ccttgtcgtc gaggaggagg gagtagcggg
     3001 cgtccccgtc cttcatgtcg atgaccttgg agctcacggt gcgcgagacg aactcgtcgg
     3061 ctccggggcc cgagacctcg atctgtccca tgtggtcgat atcgaagaga cccgctgaac
     3121 ggcgcgtgat gaggtgctcc tcgaccgcgc cggtgggata ggaaatgggc atgtcatagc
     3181 cggcgaaggg cgccatcttg gcgccgttcg cgacgtgcca gtcgtggaga cgggtcgcgt
     3241 tcagagtagt cctccttctg cttgtgcgag tgcggcctaa tctaacatga tatatcaggg
     3301 ggcgcaaggg atgcgaaacg ggaggctcag caagtgaaac ggggcagggc tcggccgagg
     3361 gcccgcctgg caccgggcct cgca
//
LOCUS       DASSQV010000381         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1457404, whole genome shotgun sequence.
ACCESSION   DASSQV010000381 DASSQV010000000
VERSION     DASSQV010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457404"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..846)
                     /gene="folE2"
                     /locus_tag="VFL04_06660"
     CDS             complement(70..846)
                     /gene="folE2"
                     /locus_tag="VFL04_06660"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HET7839424.1"
                     /translation="MIDVQAMQDDRRIPIQKVGIKGVRYPITLRDRKEGVQHTTATVN
                     LYADLPHDFKGTHMSRFIEVFEEHRRDLSMPRFLDMLEEIRSSLEAETAYADLHFPYF
                     IEKAAPVTGQTSVMSYDCAYMGKVWEGGKEFDVSVSVPVQTVCPCSKAISSHGAHNQR
                     GVVTLTVALGPFFWIEDLVSLVEAAASCDVFTLLKREDEKFVTEKAFDNPRFVEDLVR
                     EVYSEVRALGKFPKFSVEAENFESIHNHSAYAYVTSKPER"
     gene            complement(872..1753)
                     /gene="folD"
                     /locus_tag="VFL04_06665"
     CDS             complement(872..1753)
                     /gene="folD"
                     /locus_tag="VFL04_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET7839425.1"
                     /translation="MAARIIDGKKVAEDIRAEVRAGTSALASRGIVPGLAVIIVGEDP
                     ASISYVTGKERACAENGLRSFEHRLPATTSQAELLATIRACNVDPDVHGVLVQLPLPD
                     HIDEHAVMAAIDPDKDVDGFTPVNVGRLVLDHPCYVPCTPAGVVELIKRSGVPTSGRR
                     AVIVGRSNIVGKPLMNLLVRRDLNATVTVCHTGTSDLAAHTREADILIACAGRAGLLH
                     GDMIKKGACVIDVGVNRIEDHKSTKGFRLVGDVDFASAVEVAGWITPVPGGVGPMTIT
                     MLLANTLRSARSACGEV"
     gene            complement(1804..>2544)
                     /locus_tag="VFL04_06670"
     CDS             complement(1804..>2544)
                     /locus_tag="VFL04_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7839426.1"
                     /translation="WSIDMESLAAGLADPRVRVLVLINPNNPTGSYVGIEERERLVEL
                     CARRGIAIVADEVFYPFEVEAGPRSSFVGEERVLCFVLDGLSKLLGLPQMKLGWIAAS
                     GPRRELDEAISRLEIIADTYLSAGSPVMNALPRLLSGSESFCRDLRSRLRANLARLRS
                     TLEGPDSPHRVLRCEGGWSAIIESPRLETEEELAAGLLREEGLWSQPGYYFDMEREAF
                     FTASLILPPEVCAEGAGRYAEFFSRRLR"
BASE COUNT          412 a          801 c          907 g          424 t
ORIGIN      
        1 ccaatagtag agattatctc accgcggagt acgcggagtt cgcggagaat gtcgccatcg
       61 ttatatccct caccgctcgg gcttcgacgt cacgtaggcg taggccgaat ggttgtggat
      121 gctctcgaag ttctcggcct cgacggagaa tttggggaac ttgccgaggg ccctgacctc
      181 gctgtagacc tcgcgcacga ggtcctcgac gaagcggggg ttgtcgaagg ccttctcggt
      241 gacgaatttc tcgtcctccc gcttcaggag ggtgaagacg tcgcaggagg ccgcggcctc
      301 cacgagggag accaggtcct cgatccagaa gaagggcccg agggctacgg tgagggtcac
      361 gacgcccctc tggttgtggg ccccgtggga gctgatggct ttggagcagg ggcagaccgt
      421 ctggacgggg acggagaccg atacgtcgaa ctccttgccg ccctcccaga ccttgcccat
      481 gtacgcgcag tcgtagctca tgaccgaggt ctggccggtg acgggcgccg ccttctcgat
      541 gaagtagggg aagtggaggt cggcgtaggc cgtctcggcc tcaaggctcg agcggatctc
      601 ctcgagcatg tcgaggaacc ggggcattga caggtcccgg cggtgctcct cgaagacctc
      661 gatgaaacgg ctcatgtggg tgcccttgaa gtcgtggggc aggtcggcgt agaggttcac
      721 cgtggccgtg gtgtgctgga ccccctcctt ccggtcgcgg agggtgatgg gataccggac
      781 ccccttgatc cccaccttct ggatggggat gcggcggtcg tcctgcatcg cctggacgtc
      841 gatcatgggc ttcgtggctc ccccttcccc gtcagacttc gccgcaggcg gagcgcgccg
      901 acctgagggt gttcgcgagg agcatggtga tggtcatggg ccccaccccg cccggaaccg
      961 gggtgatcca gcccgccacc tcgacggccg aggcgaagtc aacgtccccg acgagacgga
     1021 agcccttcgt gctcttgtgg tcctcgatcc ggttgacgcc gacgtcgatg acgcaggctc
     1081 ccttcttgat catgtcgccg tggaggaggc cagccctccc ggcgcaggcg atcaggatgt
     1141 cggcctcgcg cgtgtgggcg gcgaggtcgc tcgtgcccgt atggcagacg gtgaccgtgg
     1201 cgttgaggtc gcggcgcacg aggaggttca tgaggggctt gccgacgatg ttcgatcggc
     1261 cgacgatgac cgcgcggcgg ccagaggtcg ggaccccgct gcgcttgatg agctcgacga
     1321 ccccggcggg agtgcagggg acgtagcagg gatggtcgag cacaaggcgt ccgacgttga
     1381 cgggggtgaa gccgtcgacg tccttgtcgg ggtcgatggc cgccatcacc gcgtgctcgt
     1441 cgatgtggtc gggcaggggg agctggacaa ggaccccgtg cacatcgggg tcgacgttgc
     1501 aggccctgat ggtcgcgagg agctcggcct ggctggtcgt ggcgggcagc ctgtgctcga
     1561 agctccgcag gccgttctcc gcgcaggcgc gctccttgcc ggtgacgtag gagatcgacg
     1621 cggggtcctc tccgacgatg atgacagcca gaccgggaac gatgccgcgc gaggcgaggg
     1681 ccgaggtgcc ggccctgacc tcggcccgta tgtcctcagc caccttcttg ccgtcgatga
     1741 tgcgagccgc catggccacc cccttttcgg tgccgccact ataccctcgg ccggctccct
     1801 gtttcaacgg aggcggcgcg agaagaactc cgcgtagcgg cccgctccct cggcgcagac
     1861 ctcgggcggg aggatgaggg aggcagtgaa gaaggcctcg cgctccatgt cgaagtagta
     1921 gccaggctgg gaccagagcc cttcctcacg gagcagcccg gcggccagct cctcctccgt
     1981 ctcgaggcgg ggcgattcga tgatggcgct ccagcctccc tcgcatcgga gcaccctgtg
     2041 gggcgagtcg gggccctcga gcgtggagcg cagccgggcc aggttcgccc ggagccggga
     2101 gcgaagatcc cggcagaagg actcggaccc cgagaggagc cggggcaggg cgttcatcac
     2161 ggggctgccg gcggaaagat aggtgtcggc gattatctcc agcctggaga tggcctcgtc
     2221 gagctcccgc cggggtcccg atgccgcgat ccagcccagc ttcatctggg gcaggccaag
     2281 gagcttggac aggccatcca gcacgaaaca gaggaccctc tcctcgccca cgaaggagga
     2341 gcggggccca gcctcgacct cgaaaggata gaagacctcg tcggcgacga tggcgatccc
     2401 cctccgggca cagagctcca cgagccgctc cctctcctcg atacccacat acgagcccgt
     2461 cgggttgttg gggttgatga ggacgaggac cctcacgcgg ggatcggcga ggccggccgc
     2521 gagggactcc atgtcgatgg acca
//
LOCUS       DASSQV010000382         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_465063, whole genome shotgun sequence.
ACCESSION   DASSQV010000382 DASSQV010000000
VERSION     DASSQV010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465063"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..565
                     /locus_tag="VFL04_06675"
     CDS             107..565
                     /locus_tag="VFL04_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7839427.1"
                     /translation="MQTVKDILDAKGSAIISIGPEASLYEALEVMADRNVGAILVVDS
                     GGGILGIFTERDFARKFIIKGRTGEGTKVKEIMTTRVLYVQPETSISDCMNLMTEKRI
                     RHLPVIVAGKVVGLVSIGDVLKATLKAQESLISQQAFEIGQLERYISGGP"
     gene            695..1852
                     /gene="earP"
                     /locus_tag="VFL04_06680"
     CDS             695..1852
                     /gene="earP"
                     /locus_tag="VFL04_06680"
                     /inference="COORDINATES: protein motif:HMM:NF021598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HET7839428.1"
                     /translation="MTIDILCKVVDNFGDIGVVYRLARALSELDGHYGLRLIVDDLGA
                     FRALDPSVDPSKPVQALHGWTVVRWDEAWEGFRAERPRIVLECFACGRPDWYEDILFD
                     PTDRERRTILSVEHLTAEAWAPDFHRLASATRSGLVRKWMFMPGFQAGTGGLVAEAGL
                     AARLEAWRRGPDRPELRRALARASGLNLPRGAEGHSWLTVFSYERDYGRIVAELSAFA
                     RDRPLLVLVAAGRSAPCFAEAWRRAGEPFPVLMLPFLRQEAWDELLMASDFAIVRGED
                     SLARAALYGLPFLWHAYLQEGAHQVVKVRALMDRMRPHFASAVFAAYEAMSIAFNDRL
                     VDGPDTRGEESILPLLGSGASMGPGFAAFSAEILGLGNLAANLVTFLGEIV"
     gene            1945..>2242
                     /locus_tag="VFL04_06685"
     CDS             1945..>2242
                     /locus_tag="VFL04_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET7839429.1"
                     /translation="MTSDIRGGTVFKAGDDVVVVQRMLGIKAGRAGQVKKLRVKNILT
                     GQTSELGLDMGEKFEEVHLDFHKMKLSYIDGDTYIFMDQESFEQYELSKDDLGDN"
BASE COUNT          396 a          778 c          715 g          353 t
ORIGIN      
        1 atcaataaaa aagcaccctc actcgccgga gaaaagaaac gttgtgcgaa tggatgcccc
       61 gcactagaat tggagacgag accattccca gcgaaggagg gagcccatgc agacggtgaa
      121 ggacatactc gacgccaagg gcagcgcgat catcagcatc ggtcccgagg cctccttgta
      181 cgaggcgctc gaggtcatgg cggaccgcaa cgtcggcgcc atcctcgtgg tcgactcggg
      241 cggcggcatc ctcggcatct tcaccgagcg agacttcgcc cgcaagttca tcatcaaggg
      301 gaggacgggg gaaggcacca aggtcaagga gatcatgacc acgcgggtcc tctacgtcca
      361 gcccgagacc tcgatctccg actgcatgaa cctcatgacg gaaaagcgga tccgccacct
      421 gcccgtcatc gtcgccggca aggtcgtggg cctcgtctcc atcggggatg tcctcaaggc
      481 cacgctcaag gcccaggaga gcctcatctc ccagcaggcc ttcgagatcg gccagctcga
      541 gcgctacata tcgggcggcc cgtaggagcc gaaggctcaa aggggcgatg gcccgtagga
      601 gccgaaggct caaaggggcg gcggcccacg ggcgccgggg ccggggggcc gcaaggcccc
      661 ccacgctgcc ccggctcgtg ctagcatgcc agacatgacc atcgacatcc tctgcaaggt
      721 agtcgacaac ttcggggaca tcggcgtcgt ctaccgcctc gcccgggccc tctcggagct
      781 ggacgggcat tacgggcttc gcctcatcgt cgacgacctg ggcgccttcc gcgccctcga
      841 tccctcggtc gacccatcca agccggtcca ggccctccac ggctggactg tcgtccgctg
      901 ggacgaggcc tgggagggct tcagggccga gaggccccgg atcgtcctcg aatgcttcgc
      961 ctgcggaagg ccggactggt acgaggacat cctcttcgac cccacggacc gggagcggcg
     1021 cacgatcctg agcgtcgagc acctgacggc cgaggcctgg gcccccgatt tccaccgcct
     1081 cgcctcggcc acgcgctcgg gcctggtcag gaagtggatg ttcatgccgg gtttccaggc
     1141 cgggaccggg ggcctcgtcg ccgaggccgg actcgcagca aggctcgagg cctggcgccg
     1201 gggtcccgac cgcccagagc tgaggcgggc cctcgcccgc gcctcgggcc ttaacctgcc
     1261 ccgtggcgcc gagggccact cctggctcac ggtcttcagc tatgagcggg actacggccg
     1321 catcgtggcc gagctctcag ccttcgcccg ggaccgcccc ctcctcgtcc tggtggccgc
     1381 gggccggagc gccccctgct tcgccgaggc ctggcgccgg gccggggagc cctttccggt
     1441 cctcatgctc cccttcctga ggcaggaggc ctgggacgag ctcctcatgg cctcggattt
     1501 cgcgatagtc cgcggcgagg acagcctcgc cagggcggcc ctctacggcc tgcccttcct
     1561 ctggcacgcc tatctccagg agggggccca tcaggtggtc aaggtgcggg ccctcatgga
     1621 ccgcatgagg ccccacttcg catcagccgt cttcgccgcc tacgaggcca tgtccatcgc
     1681 cttcaacgac aggctcgtcg acgggccgga cacccggggc gaggagtcca tcctccccct
     1741 cctcggcagc ggggcgtcaa tggggccggg cttcgccgcc ttctccgccg agatcctcgg
     1801 cctggggaac ctcgccgcga acctcgtgac attcctgggc gagatcgtgt agaatgcctg
     1861 ccgtaagctc gtcaagcatg ccgattccgc cgaaaacgcg gcgtacggct gaaaatccac
     1921 gatttgtgag gattacctat gttgatgacc tccgatatac gcggcggcac cgttttcaag
     1981 gcaggggatg acgtcgtcgt cgtccagcgc atgctcggca taaaggccgg ccgggccggc
     2041 caggtgaaga agctccgcgt caagaacatc ctgacgggcc agacctcaga gctcggcctc
     2101 gacatgggcg agaaattcga ggaggtccac ctcgatttcc acaagatgaa gctctcctac
     2161 atcgacggcg acacctacat cttcatggac caggagagct tcgagcagta cgagctctcc
     2221 aaggacgacc tcggggacaa cg
//
LOCUS       DASSQV010000383         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2205799, whole genome shotgun sequence.
ACCESSION   DASSQV010000383 DASSQV010000000
VERSION     DASSQV010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205799"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1197)
                     /locus_tag="VFL04_06690"
     CDS             complement(37..1197)
                     /locus_tag="VFL04_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839430.1"
                     /translation="MQERKSVIDLPVKLVLTEEGTTFFIRKGKNLKKFKLADNVEEYG
                     IILDSFTPASLQRMILVDYISKVEISKSELMSARQDIMDLSKLVVYSMLYRQYDSYIF
                     ARILASDVIKKWNRLNPANIIDDKTRINDSFLQTVLKDKARDLLDIKQQILAPMYAYI
                     NKNSALLPEEKNIQLLLSEKFLNNLRPFTWFIVTKFKGADGYEQLVKDLRTALAEYME
                     KSKIAEYVSLMIMELAMNAENSNLKREAKLIFKGAVDMNAVLFDPNIRRQVIESLENK
                     GELVYLSWRLGSRGVSVGTQGRLQITLYNKESEYQAVKEAFDDRKNVDLKKKSLQDFY
                     KQLPESEANTELGLYYLSYLSEACEKVNVKFESLVNQMPGSDLTVITLGLYL"
     gene            1355..>2384
                     /locus_tag="VFL04_06695"
     CDS             1355..>2384
                     /locus_tag="VFL04_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839431.1"
                     /translation="MNRLSPLIVLLACVASAGAGQAAPADLAPVSAAAESPAAQGFKA
                     RLTRLESFGRRLENSPAEAAAFDYIETVCAQAGIPAAASGFAESAEGYSFSRIVEARI
                     GGARPEELAIVVGVDSWIDSPDGSDGAAGIAFALTELEALAGARGSGRVPPVSLRFVF
                     LGAEKRGRMADGELASLGSRTWISREEGGRAMAVIYLDFDRYPGTIRLRSAGSGILSP
                     YWLYDRARRALETAGLPLSLEANRLMLNRLGLIDRYGPIRPYLDAGLPAIELSGEGGG
                     GPPAKGGAADAGAWFSGFMDSFLAASASGFPDEWDRHYIAFQIGALTAVVRETSYVAF
                     LVLFSAAAA"
BASE COUNT          350 a          753 c          801 g          480 t
ORIGIN      
        1 gaagcctcgg gtccggaccc gggccggggc ttcggcctag aggtagaggc cgagggtgat
       61 gaccgtcagg tcggagcccg gcatctggtt gacgagggac tcgaacttga cgttgacctt
      121 ctcgcaggcc tccgagaggt aggagaggta gtacaggccg agctcggtgt tggcctcgct
      181 ctcggggagc tgcttgtaga agtcctggag gctcttcttc ttgaggtcga cgttcttgcg
      241 gtcgtcgaag gcctccttga cggcctggta ctcggattcc ttgttgtaca gcgtgatctg
      301 gagccggccc tgggtcccca ccgagacgcc acgggagccg agcctccacg agaggtagac
      361 gagctcgccc ttgttctcga gggactcgat gacctgccgg cggatgttgg ggtcgaacag
      421 gacggcgttc atgtcgacgg cgcctttgaa gatgagcttg gcctcgcgct tgaggttgga
      481 gttctcggcg ttcatcgcga gctccatgat catcagggac acgtactcgg cgatcttgga
      541 cttctccatg tactcggcga gggccgtgcg gaggtccttg accagctgct cgtagccgtc
      601 ggcccccttg aacttggtga cgatgaacca ggtgaagggc cgcaggttgt tgaggaactt
      661 ctccgaaagc aggagctgga tgttcttctc ctcgggcagg agggccgagt tcttgttgat
      721 gtaggcgtac atcggcgcga ggatctgctg cttgatgtcg aggaggtccc tggccttgtc
      781 cttgagcacg gtctggagga agctgtcgtt gatcctcgtc ttgtcgtcga tgatgttcgc
      841 cgggttgagg cggttccatt tcttgatgac gtcggaggcg aggatgcgcg cgaatatgta
      901 ggagtcgtac tgccggtaga gcatcgagta cacgacaagc ttcgagaggt ccatgatgtc
      961 ctggcgggcg gacatgagct cgctcttcga gatctccacc ttggagatgt agtcgacgag
     1021 gatcatccgc tgcagggagg ctggagtgaa gctgtcgagg atgatgccgt actcctcgac
     1081 gttgtcagcg agcttgaatt tcttgaggtt cttgcccttc cggatgaaga acgtcgtccc
     1141 ttcctcggtg agcacgagct tgaccggcag atcgatgacg cttttgcgct cctgcatccc
     1201 tcgcccttca ttggccgggc gagcgtccct cgcccccatg cgccattgcc ctgatcttca
     1261 gccgccgaac cgcggctggc ggtgagtata tgagattatc ggcacgaatg taatcagtat
     1321 tgacgagggt cgcacgcctt cctatgctcg tggcgtgaac cggctctccc cgctcatcgt
     1381 ccttctcgcc tgcgtcgcct cggcgggagc cggccaggcg gcccctgcgg acctggcccc
     1441 ggtgtccgcg gcggccgagt cccctgccgc ccagggcttc aaggcgaggc tgacccggct
     1501 cgagtccttc ggaaggaggc tcgagaactc ccccgccgag gcggccgcct tcgactacat
     1561 cgagacggtc tgcgcccagg caggaatacc ggcggcggcg agcggcttcg ccgagtcggc
     1621 cgagggctat tccttttcgc gcatcgtcga ggcccgaatc ggcggggcga ggcccgaaga
     1681 gctcgccatc gtcgtcggcg ttgattcctg gatcgactcc ccggacggct ccgacggcgc
     1741 cgccggaata gccttcgccc tcaccgagct cgaggccctc gcgggggcgc gcggctcggg
     1801 cagggtcccg cccgtgtccc tccgcttcgt cttcctcggt gccgagaaac gaggcaggat
     1861 ggccgacggc gagctcgcct cgcttggctc gcgcacctgg atctcgaggg aggagggcgg
     1921 gcgggcgatg gccgtcatct acctggattt cgaccgctac cccggcacga tccggctccg
     1981 gagcgccggt tcgggcatcc tgtcccccta ctggctctat gaccgcgccc gccgcgccct
     2041 cgagaccgcc ggcctgcccc tctccctcga ggcgaaccgg ctcatgctca accgcctcgg
     2101 cctcatcgac cgctatgggc ccatcaggcc ctacctcgat gccggcctcc ccgcgatcga
     2161 gctcagcggc gagggtgggg gagggccgcc ggccaagggt ggggcggcag atgcgggggc
     2221 ctggttctcg ggcttcatgg actccttcct cgccgcgagc gcctcgggct tcccggacga
     2281 atgggacagg cactacatag ccttccagat cggggccttg accgcggtcg tgcgcgagac
     2341 gagctatgtc gccttcctcg tcctcttctc cgctgcggcg gccg
//
LOCUS       DASSQV010000384         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_837781, whole genome shotgun sequence.
ACCESSION   DASSQV010000384 DASSQV010000000
VERSION     DASSQV010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837781"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..914
                     /locus_tag="VFL04_06700"
     CDS             45..914
                     /locus_tag="VFL04_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839432.1"
                     /translation="MFSFLSGLFWLVKGVRVYALVGESGTGKSFRAKLVAQKYGIDMI
                     IDDGLLIRDETLLAGRSAKKEQFYMAAVKTALFHDKSHRDDVARALQRERFRKILVIG
                     TSDRMVRKITERLQIPHPGKTIRIEDIASRDEIEKAVRSRKVEGKHVIPVPALEIRRN
                     YPSIFYDTIRVFLKRNFGTAPARPRVYEKSVVRPEYSKRGRISISEAALSQMVVHCID
                     EFDDSVRVRRLAVRSDSQGYRLTVVLEVPFGTRLTSNAHALQEYIVDNIERFTGILIE
                     EVNIVIDRFSPRR"
     gene            994..2199
                     /locus_tag="VFL04_06705"
     CDS             994..2199
                     /locus_tag="VFL04_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839433.1"
                     /translation="MNKALIAVFALLCALSLAAAPASPTAPAAGSSFGQASSDHYIVL
                     SELGKDRATALSRQLEAYYQLYNAVFRFDPAALADKLNVREFKAKAGFDAYLGQLVGQ
                     TKDDFVYLHYPNPERSELLVFSKADPDYSASLAHQAFVQFLKHFIPNPPLWIREGLAV
                     TFESARWDDKAGKLDFPENLAWLETAKSLKERSLFMPLDRLLSIGQEEARSELDVFYP
                     QAWAFVSFLANTEDRAYNRLLWDAIAGLRKDASLDENDATVAKIVQDWYGIPESEKAF
                     ADYLASRKTFPELVADGVRLYGDKDWDGAAKSFTAAQAMNKDSYVPEYYLGLIAYAKN
                     DFATAETQYKGALGLGCDPAITNYALGVNAYAQNRIEDAKAYLVLAKQASPDRYGAKV
                     DELMAKFPK"
BASE COUNT          439 a          820 c          665 g          364 t
ORIGIN      
        1 attggcgttg ccgagccggg agagcccggt tatagtggag ggacatgttc agtttcttga
       61 gcggcctgtt ctggctcgtc aaaggcgtcc gggtctacgc cctcgtgggc gaaagcggca
      121 ccggcaaaag cttcagggcc aaactggtcg cccagaagta cggcatcgac atgatcatcg
      181 acgacggcct cctcatcagg gacgagaccc tcctcgcggg gcgctcggcc aagaaggagc
      241 agttctacat ggccgcggtg aagaccgcgc ttttccacga caagtcccac cgcgacgacg
      301 tggccagggc cctccagcgc gagcgcttcc ggaagatcct cgtgatcggg acctcggatc
      361 gcatggtcag gaagatcacc gagcggctcc agatccccca ccccggcaag accatcagga
      421 tcgaggacat agccagccga gacgagatcg agaaggcggt gcgctcgcgc aaggtcgagg
      481 gcaaacacgt catacccgtc ccggccctcg agatcaggcg gaactacccc tcgatcttct
      541 acgacaccat cagggtcttc ctcaagcgga acttcggcac ggccccggcg cggcccaggg
      601 tctacgagaa gtcggtggtg aggcccgaat actcgaagcg gggccgcatc tccatctccg
      661 aggcggccct ctcccagatg gtcgtccact gcatcgacga gttcgacgat tcggtgaggg
      721 tcaggaggct cgcggtccgc tccgactccc agggctaccg cctcaccgtg gtcctcgagg
      781 tccccttcgg gacccgcctc acctcgaacg cccacgccct ccaggagtac atagtggaca
      841 acatcgagcg cttcaccggc atcctgatcg aagaggtcaa catcgtcata gaccgcttct
      901 ccccccggag atagggggac ggtggaccgc cgccgaattt gacaggccct ttccccgaga
      961 ttacagttaa tggtgctttt ggaggtagga accatgaaca aagctctcat cgctgtgttc
     1021 gccctgctct gcgccctctc cctggccgcc gcgcccgcga gccccacggc tcccgccgcc
     1081 ggctcatcct tcggccaggc cagcagcgac cattacatcg tgctctcgga gctcggcaag
     1141 gacagggcca cggccctctc gcgccagctc gaggcctact accagctcta caacgccgtc
     1201 ttccgcttcg acccggccgc cctcgccgac aagctgaacg tccgcgagtt caaggccaag
     1261 gccggtttcg acgcctatct gggccagctc gtcggccaga ccaaggacga cttcgtctac
     1321 ctgcattacc ccaaccccga gcgctccgag ctcctcgtgt tcagcaaggc cgatcccgac
     1381 tattctgcca gcctcgccca ccaggccttc gtccagttcc tcaagcactt catcccgaat
     1441 cccccgctct ggatccgcga gggcctcgcc gtgaccttcg agtcggcccg ctgggacgac
     1501 aaggcaggca agctcgactt ccccgagaac ctcgcctggc tggagaccgc caagtccctc
     1561 aaggagcgca gcctcttcat gcccctggac cgcctgctct cgatcggcca ggaggaggcc
     1621 cgctccgagc tcgacgtctt ctacccccag gcctgggcct tcgtctcctt cctcgccaac
     1681 accgaggaca gggcctacaa ccgcctgctc tgggacgcca tagccgggct caggaaagac
     1741 gccagcctcg acgagaacga cgccacggtc gccaagatcg tccaggactg gtacgggatc
     1801 cccgagtcgg agaaggcctt cgccgactac ctggcctccc gcaagacctt ccccgagctc
     1861 gtcgccgacg gggtgaggct ctacggggac aaggactggg acggggcggc gaagagcttc
     1921 acggccgccc aggctatgaa caaggacagc tacgtccccg agtactacct gggtctcatc
     1981 gcctacgcga agaacgactt cgccaccgcc gagacccagt acaagggcgc cctcggcctc
     2041 ggctgcgatc cggcgatcac gaactacgcc ctcggggtca acgcctacgc ccagaaccgg
     2101 atcgaggacg ccaaggccta cctcgtcctc gccaaacagg cgagccccga ccgctacggg
     2161 gccaaggtgg acgagctcat ggcgaagttc ccgaaatagg cctcagggcc ccgaaaaacc
     2221 gacggccgcc ccgattaggg cggccgttgt gttgaaggac taaatcagac ttcttcggcc
     2281 gcggcggc
//
LOCUS       DASSQV010000385         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1707074, whole genome shotgun sequence.
ACCESSION   DASSQV010000385 DASSQV010000000
VERSION     DASSQV010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707074"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFL04_06710"
     CDS             <1..256
                     /locus_tag="VFL04_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839434.1"
                     /translation="LRPKLEEAARGIAAKMRAESEAIEAEAISALLKDGLRTADYPAD
                     ADARWNAVYNERGAGLVASMFSPDILELINTALAKVRKAK"
     gene            253..2205
                     /locus_tag="VFL04_06715"
     CDS             253..2205
                     /locus_tag="VFL04_06715"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET7839435.1"
                     /translation="MRGQPPSVHPEAAGSQPAAPAGILRRAAGSGLAAIAVLGLGLVV
                     FLPLIDLVLSRFFWSGLAESGPLVDHALVVLAFMAAALASLEGRHLSLTGQDQACVGL
                     RAWLYAGAQSVAAAVQASLFWASLALTWTGFDPDMRVWIIPTRLLAASIPLGFLVMGL
                     ATAFARGASRARMASACVGLLVGSLLAVSSIRNLAVALLGGSLPGLEAASELVRAGVG
                     TAILPLAGIVVLAAVLGAPLFTALGGLAVLLYVASGSYIELAPSEAYSLLRSGSIAAL
                     PLFGLAGFILAESGAGQRLVAVFRELFGWIRGGEAIAAVLACGFFSTFTGINGVTIIA
                     LGGLLVTVLSESGGFRRERARGLVTASGDIGLLLPPSAAVIVYGINAQFIYGEASAFD
                     VATLFKGAVAPGLLLVLAMCAAGVVAAPRLTKGEARRPFSPGSTLAAIGPAFLELLVP
                     LFAIVLYFTGSAGLTEIGAFCVLYIAVVEVLVKRELSPRGLLAILRRYLPIIGGTLVI
                     IAAARGLSFYIIDANVPEAFSTWISARVASPFVFLLALNIFLLMVGCLMDIYSAILVV
                     APLVIPLGTAFGVDPVHFGVIFIMNLSIGFLTPSVGINIFLASYAFRKPVARIVRDVW
                     PFLLVMLAVLLVITYVPWLSTFATHR"
     gene            2240..2692
                     /gene="rnhA"
                     /locus_tag="VFL04_06720"
     CDS             2240..2692
                     /gene="rnhA"
                     /locus_tag="VFL04_06720"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HET7839436.1"
                     /translation="MDDIVIYTDGGCSGNPGPGGWAFILEDSAGEAIGSGGEAMTTNN
                     RMELRAVIEALKAAEGRQARKEGAPVTLFTDSQYVKNGISSWIKSWKRNGWRTSDKKP
                     VKNKELWEELDAVAARLKPEFLWVEGHAGVVNNERCDSLVQDEIRLFR"
BASE COUNT          362 a         1150 c          903 g          499 t
ORIGIN      
        1 gctgaggccc aagctcgagg aggcggcccg cggcatcgca gccaagatgc gcgccgagag
       61 cgaggcgatc gaggccgagg ccatcagcgc cctcctcaag gacggcctca ggactgcgga
      121 ctacccggcg gacgccgatg cgcgctggaa cgcggtctac aacgagcgcg gggcgggcct
      181 cgtcgcctcc atgttctccc ccgacatact cgagctcatc aacacggcgc tcgccaaggt
      241 gcggaaggcc aagtgagggg ccagccgccc tcggtacatc cagaagccgc cggaagtcag
      301 ccggccgcgc cggcgggcat cctgcggagg gccgcgggct cgggcctggc cgccatcgcc
      361 gtcctgggcc tcggcctcgt ggtcttcctc cccctcatcg accttgtcct ctcccgcttc
      421 ttctggagcg gcctcgccga atccggcccc ctggtcgacc acgccctcgt cgtcctggcc
      481 ttcatggccg cggccctggc atccctcgag ggcaggcacc tctcgctcac gggccaggac
      541 caggcctgcg tggggctcag ggcctggctt tacgcgggcg cccagtccgt cgcggcagcc
      601 gtgcaggcga gcctcttctg ggcctcgctc gccctcacct ggacgggctt cgaccccgac
      661 atgagggtct ggatcatccc caccaggctc ctcgccgcct cgatccccct cggcttcctc
      721 gtcatgggcc tcgccaccgc cttcgcgcgc ggagcctcgc gggcccgtat ggcctcggcc
      781 tgcgtcggcc tcctggtggg cagcctcctc gccgtctcct cgatccgcaa cctcgcggtc
      841 gccctccttg gcggctccct gcccggcctc gaggcagcga gcgagctcgt ccgcgccggg
      901 gtcggcacgg cgatccttcc cctcgccggc atcgtcgtcc tcgctgcggt gctgggcgcc
      961 cccctcttca ccgccctcgg cggcctcgcc gtcctcctct acgtggcctc ggggtcctac
     1021 atcgagctcg cgccgagcga ggcctactcc ctcctgcgct caggctcgat cgccgccctt
     1081 cccctcttcg gcctcgccgg cttcatcctc gccgagtcgg gggccggcca gcgtctcgtg
     1141 gccgtcttcc gcgagctctt cggctggatc aggggcggtg aggcgatcgc cgcggtcctc
     1201 gcctgcggct tcttcagcac cttcacgggg atcaacgggg tcacgatcat cgccctcggc
     1261 ggcctcctcg tgaccgtgct ctcggagtcc ggcggcttca ggagggagcg ggccaggggc
     1321 ctcgtcaccg cgagcggcga catcggcctt ctgctgccgc cgagcgcggc cgtcatcgtc
     1381 tacggcatca acgcccagtt catctacggc gaggcctcgg cctttgacgt ggccaccctc
     1441 ttcaagggcg cggtcgcccc cggcctcctc ctcgtgctcg cgatgtgcgc agcaggggtg
     1501 gtggcggcgc cacggctcac caagggagag gcgcgccggc ccttctcccc gggctcgacc
     1561 ctggccgcca tcggcccggc cttcctcgag ctcctcgtgc ccctcttcgc catcgtcctg
     1621 tatttcacgg gctcggccgg cctcaccgag atcggggcct tctgcgtgct ctacatcgcc
     1681 gtcgtcgagg tcctcgtgaa gcgcgagctc tcgccccgtg gactcctggc catcctcagg
     1741 cgctacctcc cgatcatcgg cggcaccctc gtcatcatcg ccgcggcgcg cggcctctcc
     1801 ttctacatca tcgacgccaa cgtgcccgag gccttctcca cctggatctc ggcgagggtg
     1861 gcctccccat tcgtgttcct cctcgccctc aacatcttcc tcctcatggt cggctgtctc
     1921 atggacatct actcggcgat cctcgtcgtg gcccccctcg tcatccccct cggcacggcc
     1981 ttcggcgtcg accccgtgca cttcggcgtg atcttcatca tgaacctctc gatcggtttc
     2041 ctcaccccga gcgtcggcat caacatcttc ctcgcgagct acgccttccg gaagcccgtg
     2101 gcccggatcg tcagggatgt ctggcccttc ctcctcgtca tgctcgccgt gctcctcgtc
     2161 atcacctacg tcccctggct ctcgaccttc gcgacccatc gttgactccc cggctcccga
     2221 aaaggcatac tcgcttccca tggacgacat cgtgatctac accgacgggg gctgctcggg
     2281 caacccgggc cccggaggct gggctttcat cctcgaggac tcggccgggg aggcgatcgg
     2341 ctcgggcggc gaggccatga cgaccaacaa ccgcatggag ctccgcgccg tgatcgaggc
     2401 gctcaaggcc gccgagggcc gccaggcccg caaggagggc gctccggtga ccctcttcac
     2461 cgactcgcag tacgtgaaga acgggatcag ctcctggatc aagtcctgga agcgcaacgg
     2521 ctggcggacc tcggacaaga agccggtcaa gaacaaggag ctctgggagg agctcgacgc
     2581 cgtcgcggcg aggctcaagc ccgagttcct ctgggtcgag ggccacgccg gcgtcgtgaa
     2641 caacgagcgc tgcgattccc tcgtgcagga cgagatccgc ctcttccgct gacccccggc
     2701 gccccgggga tcgggcggcg ccgccacgcg cggcgttccg gcggcctccc tagccgaggg
     2761 ctatcgccgt ctccagctcg tggaggaaga gcccggccgc gagcatgtcg gccgagccgc
     2821 ctgggctcag gccctctgag cagagcagct cgtcgaagcg ggcgagggcc tcggctcccg
     2881 cctcgctccc cgccccaccc gccagaagca cccc
//
LOCUS       DASSQV010000386         2803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2081621, whole genome shotgun sequence.
ACCESSION   DASSQV010000386 DASSQV010000000
VERSION     DASSQV010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081621"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFL04_06725"
     CDS             <1..345
                     /locus_tag="VFL04_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839437.1"
                     /translation="GLDRVSLFPDGRGLAVLSSGATMKVKASIVASTLVVVQDQPSIP
                     EFYRSPGMDLKSARQVAAAARPWRWIFSLALDGNSLSGVKESVFVKVDAQGSVSVDNN
                     YVRDAAWKRLYR"
     gene            complement(348..833)
                     /locus_tag="VFL04_06730"
     CDS             complement(348..833)
                     /locus_tag="VFL04_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839438.1"
                     /translation="MSLRYSPRARLGRARAAGLAPAIALSVFAGLAVYCALSILAGQA
                     GLFAYRGLEERKQLMVGNLARLGSLNERLREELEALRSDSGRSSREARALGYLGRDER
                     EVVIMGRQEERRGYETGSVVPFDQAPALPDLAAKELAFGAALAVLALGLAPRSRRRGG
                     A"
     gene            1197..>2803
                     /locus_tag="VFL04_06735"
     CDS             1197..>2803
                     /locus_tag="VFL04_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839439.1"
                     /translation="MPEESFLSEDEERLLGSFDSEFEAGFEESPRTPVLDEGTLPDIE
                     DMPPLEEALLENPTGAIPDIGDLASGAIDIRLEDIDRPFGEPHISPSAELDMSTVRGL
                     DAEPGAKGPSPAPGAMEDVSSEFLDVLSQPASAEAGADAKAGDSDDVTSQFLDFEEEA
                     SPHAEETPSPDFEPIDIDLQFDDTIPMTGSESDNSLAAGFEPVTEFDDFLSAEHEAEA
                     AEAKPASSPAPEAEAETFDDLAAVERDLAEKPETRHAEAKAGSDLSTEILLKIADELS
                     SIRGELVSLKTQLGSLKSKAPVLEESPLPEAPVKEEASGGFFDDEEDETIALTGDELD
                     NILNTADFTEEVAEAEQPEEIGAGMDLEAAGAPAELDILADGLLPESGDYAAREESGI
                     EEVRIGAQKTGEEAFLDEELSEEEIPSIEDISTIAEQGISPVTEAPEDTSYLETELGE
                     LGLSEAPLSEAPLVEPDLSDFDIEEEELQAGAIAEIEEELPVVEPSAEDLVLEAEAKL
                     DFGEAESIGELEPLEPIPEIDDSAFGE"
BASE COUNT          509 a          967 c          929 g          398 t
ORIGIN      
        1 ggcctcgaca gggtctcgct tttccccgac ggccgcggcc tcgcagtcct ctcgtcgggc
       61 gcgaccatga aggtgaaggc gagcatcgtg gcctcgaccc tggtggtcgt ccaggaccag
      121 ccgagcatcc cggagttcta ccgcagcccc gggatggacc tcaaatcggc gcgccaggtg
      181 gcggcggctg ccaggccctg gaggtggatc ttctcgctcg cgctcgacgg caacagcctg
      241 tcgggggtca aggagtcggt cttcgtgaag gtcgatgccc agggctcggt gagcgtcgac
      301 aacaattacg tgcgcgacgc cgcctggaaa aggctgtaca ggtaagccta ggctccgccg
      361 cgccggcgcg agcggggggc gaggccaagg gcaaggactg ccagggcggc cccgaaggcg
      421 agctccttcg ccgccaggtc gggcagggcg ggcgcctggt cgaaggggac caccgagccc
      481 gtctcgtagc cccggcgctc ctcctgccgc cccatgatga cgacctcgcg ctcgtcgcgg
      541 ccgagatagc caagggccct cgcctctcgg gatgagcggc ccgagtcaga cctgagggcc
      601 tcgagctcct ccctgagcct ctcattcagg gatcccagcc tggcgaggtt gcccaccatc
      661 agctgcttcc gctcctcgag gccgcgatag gcgaagagcc ccgcctgacc ggcgaggatg
      721 gagagggcgc agtagacggc cagcccagcg aagacggaaa gggcgatggc cggggcgagg
      781 ccggcggccc ttgcgcgccc aagccgcgcg cggggggagt atcgaaggct catctggcat
      841 attaacggcc gtcttggccc ttcccttgaa tccctcgaac ccgattcggg gctcccgggc
      901 cgcaggagga ccctattatt gacaatggcg ccggtggcgc atagagttga tcgcaggtcc
      961 gttaatgcgc agagtcaacc tgccgatact ctacaaagat cgcccgtaac ggagttgtca
     1021 tggccaaacg tcccgaaacc gaacccaccc gtgcagcgcc taagcctgga agctcgaagg
     1081 gcgaactgga gcaatacgga gtctgggtga aggcggagcc gcaagatatt gtccaggatg
     1141 cgccggcttc cctcgccgcg gatttcgatg cgacgttgcc gggaggtgcg gagctcgtgc
     1201 ccgaggagtc attcctctca gaggacgagg agcgcctctt gggttccttc gactcggagt
     1261 tcgaggccgg tttcgaggag agtccccgca cccccgtcct tgacgagggc acccttcccg
     1321 acatcgagga catgcctccc ctcgaggaag ccctcctcga gaaccccacg ggcgcgattc
     1381 ccgacatcgg ggacctggcc tccggggcca tcgacatacg gctcgaggac atcgaccgcc
     1441 ccttcggaga gccgcacatc agcccaagcg ccgagctcga catgagcacc gtgcgcggcc
     1501 tcgacgccga gcctggggcc aagggcccct cccccgctcc cggcgccatg gaggacgtct
     1561 cctcagagtt cctcgatgtc ctctcccagc ccgcctcggc cgaggctggg gccgacgcca
     1621 aggccgggga ctccgacgac gtgacctcgc agttcctcga cttcgaggag gaggcgagcc
     1681 cccacgccga ggagaccccg tctccggatt tcgagcccat cgacatcgac ctccagttcg
     1741 acgacaccat ccccatgacg ggttcagagt ccgataactc cctcgccgcc ggctttgagc
     1801 cggtgacgga gttcgacgac ttcctgagcg ccgagcacga ggccgaggcc gccgaggcca
     1861 aaccggcctc aagcccggcg ccggaggcgg aggccgagac cttcgacgac ctcgcggcgg
     1921 tggagcgcga tctagccgag aagcctgaaa ccaggcatgc ggaggccaag gcggggagcg
     1981 acctctcgac cgagatcctc ctcaagatcg ccgacgagct ctcctcgatc cgaggcgaac
     2041 tggtgtccct caagacccag ctcggctccc tcaagtccaa ggcccccgtc ctcgaggaaa
     2101 gcccccttcc cgaggccccc gtcaaggagg aggcctccgg aggcttcttc gacgacgagg
     2161 aggacgagac gatcgccctc accggggatg agctcgacaa catcctgaac accgccgatt
     2221 tcaccgagga ggtggccgag gccgaacagc ccgaggagat cggggcgggc atggatctcg
     2281 aggcggcagg ggcacccgct gagctcgaca tcctcgccga tggcctcctt cccgagagcg
     2341 gagactatgc ggcgcgcgag gagtccggga tagaagaggt caggatcggc gcccagaaga
     2401 caggggagga ggccttcctc gacgaggagc tttccgaaga ggagatcccc tcgatcgagg
     2461 atatcagcac catcgccgag cagggcatca gccccgtgac cgaagccccc gaggacacca
     2521 gctatctcga gaccgagctc ggcgagctcg gcctctccga ggccccgctc agcgaggccc
     2581 ccctcgtcga gcccgacctc tccgatttcg acatcgagga ggaggagctc caggccggcg
     2641 cgatcgccga gatcgaggag gagctgcccg tggtcgagcc ctcggccgag gacctcgtcc
     2701 tcgaggccga ggcgaagctg gacttcggcg aggccgagtc gatcggcgag ctcgagcccc
     2761 tcgagcccat ccccgagatc gatgacagcg ccttcggcga gat
//
LOCUS       DASSQV010000387         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1956597, whole genome shotgun sequence.
ACCESSION   DASSQV010000387 DASSQV010000000
VERSION     DASSQV010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956597"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VFL04_06740"
     CDS             <1..1260
                     /locus_tag="VFL04_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7839440.1"
                     /translation="EALAMLRREIAGQEGYLRELENEVFLEEKRLGEAQEELGDIDGE
                     IADIEKKGRELTAELERLDREIALKNSDLSKRQEDLKKKMDRLAGLQGELERLHLGLA
                     QTETEIRNVKENFAEQYSRDLMEFEARMFEIRTPVAELRETQTGLKQRLKDLGSVNLM
                     APEEYAEVKERYDFLSGQIADLEKARADLKRITDEIRDESAALFLETYNKIKKNFHNM
                     FRRLFGGGRGELRLVDPNHILESGIEIYAQPPGKKLEAISLLSGGEKTLTAIALLFAT
                     YMVKPSPFCFLDEIDAALDEQNVIRFVNLLREFGRTSQFVVITHNKKTVTGADTLLGV
                     TMEESGITKVITVRLERSDGKPISRLEPVVAMVEEEVEYEEGTELPTEPESPKASKPE
                     ARPQAEARTEARAPSPEPLPSASGGEA"
     gene            1264..1833
                     /locus_tag="VFL04_06745"
     CDS             1264..1833
                     /locus_tag="VFL04_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839441.1"
                     /translation="MAASPLAAATAALARVWRGILDALPFVKRGGGAKSSEEPFSTIE
                     DPTSTSDMLLEANAAMTGAAAPREARIDLRASLAALAKNTVLVVSLLAILGFLLVLAV
                     TAIIVGAPPKPMPAAPALSREGTDLVKTWIYPPGLSLEPRMPMEREGRPKYSYGDSAR
                     IGIDPAKVDLAPLKATNDEAIDELYRTVR"
     gene            1830..>2152
                     /locus_tag="VFL04_06750"
     CDS             1830..>2152
                     /locus_tag="VFL04_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839442.1"
                     /translation="MTKLGRCSILILIAAVCGLGSCVSTPPASPAKPEAAAGPPGAPA
                     APAATPAAAPAAMAEASFPRRVAMSGRLIVASLPEPGLPGQVLGEMPMVAGRRSPPKQ
                     ALPVAA"
BASE COUNT          387 a          793 c          689 g          283 t
ORIGIN      
        1 gaggccctgg ccatgctccg gcgcgagatc gccgggcagg agggctacct ccgcgagctc
       61 gagaacgagg tcttcctcga ggagaagcgg ctcggcgagg cccaggagga gctcggcgac
      121 atcgacggcg agatagccga catcgagaag aagggccgcg agctcacggc cgagctcgag
      181 cgcctggacc gcgagatcgc tttgaagaac tccgacctgt ccaagcggca ggaggacctc
      241 aagaagaaaa tggaccggct cgcaggcctc cagggcgagc tcgaacgcct ccacctgggc
      301 ctcgcccaga cggaaaccga gatccgcaac gtcaaggaga acttcgcgga gcagtactcg
      361 cgcgacctca tggagttcga ggcgaggatg ttcgagatcc gcacgcccgt cgcggagctt
      421 cgcgagaccc agaccggcct caagcagcgg ctcaaggacc tcggctcggt caacctcatg
      481 gcccccgagg aatacgcgga ggtgaaggag cggtacgact tcctctccgg ccagatcgcc
      541 gacctcgaga aggcgagagc cgacctcaag cgcataacgg acgagatccg cgacgagagc
      601 gccgcgcttt tcctcgagac ctacaacaag atcaagaaga acttccacaa catgttccgc
      661 cgcctcttcg gcgggggcag gggggagctc cgcctcgtcg acccgaacca catcctcgag
      721 tcgggcatcg agatctacgc ccagcccccg gggaagaagc tcgaggccat ctcactgctc
      781 tccggcggcg agaagacgct taccgcgatc gccctgctct tcgccaccta catggtcaag
      841 ccgagcccct tctgcttcct cgacgagatc gacgcggccc tcgacgagca gaacgtcatc
      901 cgcttcgtga acctcctgcg cgagttcggc cgcacgagcc agttcgtcgt gatcacgcac
      961 aacaagaaga ccgtgaccgg ggccgacacc ctcctcggcg tgaccatgga ggagtcgggc
     1021 atcaccaagg tcatcacggt gaggctcgag cgctccgacg gcaaaccgat cagccggctc
     1081 gagcccgtcg tcgccatggt cgaggaggag gtcgagtacg aggagggcac cgagctcccg
     1141 accgagcccg agtcccccaa ggcctccaag cctgaagcca ggccccaggc cgaggcccgg
     1201 accgaggcgc gcgccccgag ccccgagccc ctgccatcgg cgtcgggagg ggaggcctag
     1261 gccgtggcgg cttcgcctct cgcagcggcc actgccgcgc tcgcgcgggt ctggcggggc
     1321 atccttgatg ccctgccctt cgtcaagcgg ggagggggcg ccaagagctc cgaggagcca
     1381 ttcagcacga tagaggaccc cacctcgacc tcggacatgc tcctcgaggc gaacgccgcg
     1441 atgacgggag ccgccgcgcc gagggaggcg cgcatcgacc tccgggccag cctggcggcc
     1501 ctggccaaga acaccgtcct tgtggtctcg ctcctcgcca tcctcggctt cctcctcgtc
     1561 ctcgcggtga ctgccatcat agtcggcgcc ccgccgaaac cgatgcccgc ggcccccgcc
     1621 ctcagccgcg aggggaccga cctcgtcaaa acctggatct atcctcccgg cctgagcctc
     1681 gagccgagga tgccgatgga gagggaaggc cggccgaagt actcgtacgg ggactcggcc
     1741 cggatcggga tcgacccggc caaggtcgac cttgccccgc tgaaggccac gaacgacgag
     1801 gcgatcgacg aactctacag gacggtgcga tgacgaaact cggccgatgc tccatcctca
     1861 tcctcatcgc ggccgtctgt ggcctcggct cctgcgtctc aaccccgccg gcctcgcccg
     1921 cgaagccgga ggcggccgcc ggcccgcccg gggcccccgc cgctccggcg gccaccccgg
     1981 cagccgctcc ggcggccatg gccgaggctt cattcccgcg gcgcgtcgcc atgtcaggcc
     2041 gcctcatcgt cgcctccctc cccgagcccg gcctgcccgg ccaggtcctc ggcgagatgc
     2101 ccatggtggc aggacgccgc tccccgccca agcaggccct tcccgttgcg gc
//
LOCUS       DASSQV010000388         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2331279, whole genome shotgun sequence.
ACCESSION   DASSQV010000388 DASSQV010000000
VERSION     DASSQV010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331279"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1751)
                     /locus_tag="VFL04_06755"
     CDS             complement(<1..1751)
                     /locus_tag="VFL04_06755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HET7839443.1"
                     /translation="MRASPREILKQVFGYDEFRPLQSEIIEAVLARRDVLAVMPTGGG
                     KSLCYQVPALVDNGSGGGMVLVVSPLIALMRDQVAFLRECGVEARVLNSSLSPEEWRA
                     NAEAARRGELRLLYLAPETLNSPRFRELLDALVAQTLIRLFAVDEAHCISEWGHDFRP
                     DYRSLGALREGLCGVPCLALTATATERVRADIRKELRLDSPLELVAGFDRPNITLEVR
                     RRGRVVEQLAALASEYPGRSGIVYCFSRARAEELAQGLSRRGVSALPYHAGLPPETRS
                     KNQDLFIADEVRVIAATTAFGMGIDKPDVRFVAHADLPKSLEQYYQELGRAGRDGLPA
                     RAVLYFGFGDVMKIRALLANGSGGAWDEYGADEADPEAEAARAARLASAEASLRSMLR
                     YAEAASCRRVSLLAHFGERYPSAGCGSCDVCLGLGEEADEVDATVQAQKLLSCVKRTG
                     ERFGAGYVVDVLLGSRNERVLGLGHTELSTWGIGKEWTRGQWLDLARQLLRKGYLAKD
                     EEYGVLSLTAKAYEAFRDKTPILASIPPRGKKEKAGAAAARGGGAGGPSLPLGEGSDA
                     EGERLATALRGLRKRLA"
     gene            1920..>2574
                     /locus_tag="VFL04_06760"
     CDS             1920..>2574
                     /locus_tag="VFL04_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HET7839444.1"
                     /translation="MLLMSKPIVFRFPLLVFVLAAGLGFLAACDLIPGSGGTDPVVPV
                     TPTIEAPATRFEASSHAPSVIVSLTSSLGASIYYTLNGTTPSSASIHYNAPFSLKQTT
                     RVKAIAVNSSGASSIATQLFLFKRTTQELFSTDGTVTERMDYHYDPAGYFCDCIEDYQ
                     GSPLAYFQKFVYTYDPATGKRARRDTYNASNVLTGYRTFSFDSTTGRNNRRDFYNASG
                     "
BASE COUNT          424 a          885 c          855 g          410 t
ORIGIN      
        1 tcggcgaggc gtttgcggag gccgcgcagg gccgtggcga gccgctcgcc ctcggcgtcg
       61 gagccctctc cgaggggcag gctcggcccg ccggctcccc cgccccgcgc cgccgctgcc
      121 cctgccttct ccttcttccc gcggggcggg atcgaggcga ggataggcgt cttgtcccgg
      181 aaggcctcgt aggccttggc ggtgagcgag aggaccccgt actcctcgtc cttcgcgaga
      241 tagcccttgc gcaggagttg gcgggcgagg tcgagccact ggccccgcgt ccactccttc
      301 ccgatgcccc aggtcgagag ctcggtgtgg ccgagaccga ggacgcgctc gttcctcgag
      361 ccgaggagaa cgtcgacgac atagccggcg ccgaagcgct cgcccgtccg cttcacgcag
      421 gagaggagct tctgggcctg gaccgtggcg tcgacctcgt cggcctcctc gccgaggccg
      481 aggcagacgt cgcaggagcc gcagccggcc gagggatagc gctcgccgaa atgggcgagg
      541 aggctcaccc ttctgcagct cgcagcctcg gcgtaacgga gcatggatcg cagcgaggcc
      601 tcggccgagg ccagccgggc ggcgcgcgcc gcctcggcct cgggatcggc ctcgtcggcc
      661 ccgtactcgt cccaggcccc gcccgagccg ttggccagga gggccctgat cttcatgacg
      721 tcgccgaagc cgaagtagag gacggcgcgg gcgggcagac cgtcccggcc ggcgcgcccg
      781 agctcctggt agtactgctc gaggctcttg ggcaggtcgg catgggccac gaagcggacg
      841 tcgggcttgt cgatgcccat gccgaaggcc gtggtcgcgg cgatcaccct gacctcgtcg
      901 gcgatgaaga ggtcctggtt cttggacctg gtctcggggg gaaggcccgc gtggtagggg
      961 agggcgctca cgccccgccg gctgaggccc tgcgccagct cctcggccct ggcccgcgag
     1021 aagcagtaca cgatgcctga gcgacccggg tactcggagg cgagggcggc cagctgctcg
     1081 acgaccctgc cgcgccgcct cacctcgagg gtgatgttgg gcctgtcgaa acccgcgacc
     1141 agctcgaggg gggagtcgag gcggagctcc tttctgatgt cggcgcggac ccgctcggtc
     1201 gccgtcgccg tgagggccag acagggcacg ccgcacaggc cctcgcggag ggcaccaagg
     1261 ctccggtagt cgggcctgaa gtcgtggccc cactccgaga tgcagtgggc ctcgtccacg
     1321 gcgaagaggc gtatgagggt ctgggccacg agagcgtcga ggagctcgcg gaagcggggc
     1381 gagttgaggg tctcgggcgc gagatagagg agcctgagct cgcccctccg cgcggcctcg
     1441 gcgttcgccc tccattcctc ggggctcagg gaggagttga gcaccctggc ctcgaccccg
     1501 cactcgcgga ggaaggccac ctggtcgcgc atgagggcga tgagcggcga gaccacgagg
     1561 accatgcccc cgccagatcc gttgtcgacg agggcgggca cctggtagca tagggatttg
     1621 ccgcccccgg tgggcatgac ggcgaggacg tcccgcctgg cgagcacggc ctcgattatc
     1681 tcgctctgga ggggccggaa ctcgtcatag ccgaagacct gcttgagtat ctcacggggg
     1741 ctcgcgcgca tcacggccga gtatagcccg ggccgacagc tcgcgtcagc acgctggggg
     1801 accttcagcg cggacgcgaa aaaaaacgct tgccaaaccg cgaaacggat atacagtgac
     1861 cctaaccatg ccgaatgaga gtgagtaaat attacttaat aaataaggca tggttggagg
     1921 tgctcctcat gagcaagccc attgtcttcc gtttccctct cctggtgttc gtcctcgccg
     1981 ccggccttgg ctttctcgcg gcctgcgacc tcatccctgg ctcgggcggc accgaccccg
     2041 tcgttcccgt taccccgacc atcgaggctc cggcgacccg cttcgaggct tctagccacg
     2101 caccgagcgt gatcgtgagc ctgacgagct ccctcggcgc gagcatctac tacaccctga
     2161 acggtacgac gcccagcagc gccagcatcc actacaatgc ccccttcagc ctcaagcaga
     2221 cgaccagggt caaggcgatt gcggtcaaca gttccggcgc ttcctccatc gccacccagc
     2281 tcttcctctt caagcgcacc acgcaggagc ttttctcgac agatggcacg gtcaccgagc
     2341 gtatggacta tcactacgat cccgccggtt acttctgcga ctgcatcgag gactatcagg
     2401 gatcgcccct cgcctacttc cagaaattcg tctataccta cgatccagcg acgggaaagc
     2461 gtgcgcgaag ggatacctat aatgcgtcca acgtcctaac gggctaccgc actttcagct
     2521 tcgattccac caccggccgc aataacagaa gggacttcta caacgcctcc ggcg
//
LOCUS       DASSQV010000389         4273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_466649, whole genome shotgun sequence.
ACCESSION   DASSQV010000389 DASSQV010000000
VERSION     DASSQV010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466649"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..2186
                     /locus_tag="VFL04_06765"
     CDS             246..2186
                     /locus_tag="VFL04_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HET7839445.1"
                     /translation="MDYAKDGALARMFPKASVELKFGIERRSFPLVAEDSSDGGILYR
                     NEWIELRGEMQSLPDGSRYRLHLRSLSRKSIRITRLRFPAEGGIGPFLEGFHPTRVAF
                     LRNGYQSWSTARSYRISEKPLRPRLGLVSLATSNMANLPSNTAGELSSERYSVITDLT
                     DGSSMLVGQEPPFDQFFYIALNVDKRGGSFFELSYDFGRQMMNPGERLDLDGIVFLYG
                     TRPKVEQSYFHMIREETGYKAPTSNLRGWCSWYQYYDRITPDILYRNLEALKRRGLGF
                     DFFQIDDGWQAAVGDWLEQSPPFKGRMKELADAIRAAGMRPGLWFAPFAAASDSELFR
                     LHPEYVLKTEYGRFLKAGYNPIWKGYYYGLDATHPRYAEYLREVVHTIVDEWGFDYLK
                     CDFLFSACLRGGAHHELAISRSRILKEGMALIREEAGREAVIIGCGMPISAGIGMVDA
                     MRVGPDTGDFWIKLAGKLLRTGAMVGARNSIRNFMVRSPMHKRLWLNDPDCVMIRDTD
                     TRLKDPERYAQMDAIALSGGILMYSDDFSRLSERAFRDMALIDEVSDACFQGQAIAVD
                     VMEKELPEIYYNTSGHVGFFNFRGRARRRFDLGRLRDYEPGLSALLDLRTGERLRPSG
                     GVLDLGLMPRHGSRLFRIERGV"
     gene            2273..2443
                     /locus_tag="VFL04_06770"
     CDS             2273..2443
                     /locus_tag="VFL04_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7839446.1"
                     /translation="MAYKVTDACVNCGACEPECPVSAISEKNGARVIDAATCIDCGSC
                     ASVCPTEAILAP"
     gene            2604..3521
                     /locus_tag="VFL04_06775"
     CDS             2604..3521
                     /locus_tag="VFL04_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839447.1"
                     /translation="MSGIRDLGTGNVARAEGRAAFDARGGLESLLTHLVSESYLLLLR
                     SVKSSHDSMRTIYSEWNRGELADPLIAATADALGLRDLDGEPLLEKVLDVASRAEPCV
                     NAAALAMDLGVPAPAFAQAAASSHISALKDERVDASAVLSGPKHATTGDRTALVEEIR
                     RALLAATILAQAQAFLLLSRAAAAKGALADLCGTAQLWSEGRASFLHQRLGEALCRNP
                     ELELVILDAQIKALLDPCLPSLRRVVARSAESGLAVPVFAACLQFYDQCRSTWLPANL
                     VRALRDARGGEGFERVDRPRGEIFHSDWK"
     gene            3560..>4273
                     /locus_tag="VFL04_06780"
     CDS             3560..>4273
                     /locus_tag="VFL04_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839448.1"
                     /translation="MPKRTSLSMAGLCLAVALLAGMAAVFGVFFRGDGSSATAISVRG
                     EQFDYATNGVYRYNAQRVVAEGVGWDVFTLFCAVPAMLACLPALARGSFRGRLFALGL
                     LGYFFYQYLEYSLTWAYGPLFLPFIAIYALSLAGIAWIAPSVGLAGLSRRFGPGFPRR
                     GMAVLCFAMAAILLVMWLSLIIPTMGGSAVGLLKGQTTLVVQALDLGLIVPLCLFTGI
                     AAWRARPIGYLLCSVFVVKA"
BASE COUNT          669 a         1533 c         1342 g          729 t
ORIGIN      
        1 gggacgaagg gggtcaccac gtaggtcggg ccgagggtcc tgttgagcca gtaggcgtag
       61 ttcctcacgg tgatcgtcac gccgtccatg atgggcatgt agctgtcgtt gaacaggcct
      121 attgtgacgt ccttcatttc cgacaaggat accccagcgg ggctcccgcg tcaacgaagg
      181 ggccgggcgg ccctcgcttg cgcgggagcg gcggccgcat atagttgaag cggaaggcgg
      241 cccacatgga ctacgcgaag gatggcgcgc tcgcgcggat gtttcccaag gcctcggtcg
      301 aactcaagtt cggcatcgag cggcggtcct tccccctcgt ggccgaggac agcagcgacg
      361 gcggcatcct ctacaggaac gaatggatcg agctcagggg cgagatgcag agcctccccg
      421 acggctcgcg ctaccgcctc cacctccgca gcctgagccg gaaatccata aggatcaccc
      481 gcctccgctt ccccgccgag ggcggcatcg ggcccttcct cgagggcttc caccccaccc
      541 gcgtcgcctt cctgcgcaac ggctaccagt cctggtcaac ggcccggtcc taccggatct
      601 ccgagaagcc cctgcgcccc cggctgggcc tggtctccct cgcgacctcc aacatggcta
      661 acctgccctc gaacactgcc ggggagctct cctctgagag gtactccgtg atcaccgacc
      721 tcaccgacgg gagctcgatg ctcgtgggcc aggagccgcc cttcgaccag ttcttctaca
      781 tcgcgctcaa cgtcgacaag aggggaggca gcttcttcga gctgagctac gacttcggcc
      841 ggcagatgat gaaccccggc gagcggctcg acctcgacgg gatcgtcttt ctctatggca
      901 ccaggcccaa ggtcgagcag tcctatttcc acatgatccg cgaggagacg ggctacaagg
      961 caccgacatc caacctcagg ggctggtgct cgtggtacca gtactacgac aggatcactc
     1021 ccgacatcct ctacaggaac ctcgaggccc tgaagcgcag gggcctgggc ttcgacttct
     1081 tccagataga cgacggctgg caggccgcgg tcggggactg gctcgagcag agtcccccct
     1141 ttaagggcag gatgaaggag ctcgccgatg cgatcagggc cgcggggatg cggcccgggc
     1201 tctggttcgc ccccttcgcc gccgcgagcg attcggagct attccgcctc caccccgagt
     1261 atgtgctcaa gaccgagtat ggccgcttcc ttaaagccgg ctacaacccg atctggaagg
     1321 gctactacta tggcctcgac gccacccacc cgcgctacgc cgaatacctc agggaggtcg
     1381 tccacacgat cgtggacgag tggggcttcg actacctcaa gtgcgacttc ctcttctcgg
     1441 cctgcctcag gggcggggcc caccacgagc tcgccatctc ccggtcgagg atactcaagg
     1501 aggggatggc gctcatccgc gaggaggccg gcagggaggc ggtgatcatc ggctgcggga
     1561 tgccgatctc ggcggggatc ggcatggtgg acgccatgcg ggtggggccc gacacggggg
     1621 acttctggat caagctcgcg ggcaagctcc tgcgcaccgg ggccatggtg ggggcgagga
     1681 actccatccg caacttcatg gtccgcagcc ccatgcacaa gaggctctgg ctcaacgacc
     1741 cggactgcgt gatgatccgg gacacggaca cgaggctcaa ggatcccgag cgctacgccc
     1801 agatggacgc aatcgccctg tctggaggga tcctcatgta ctccgacgac ttctcccggc
     1861 tctcggagcg cgccttccgc gacatggccc tcatcgacga ggtgtccgac gcctgcttcc
     1921 agggccaggc catcgccgtc gacgtcatgg agaaggagct ccccgagatc tactacaaca
     1981 cctcgggcca tgtcggtttc ttcaatttcc ggggacgcgc gcggcgccgc ttcgaccttg
     2041 ggcggctcag ggactacgag cccgggctct cggccctgct cgacctcagg accggggaga
     2101 ggctcaggcc ctcgggcggg gtcctggacc tcggcctcat gccccgccac ggctcgcggc
     2161 tcttccgcat tgagcgcggg gtctaggaga ccagggccgc cggtttgagt gcttgccaag
     2221 gaagggaagg ggggatatac tccaaatact ccattcccaa gagaggtatt ccatggccta
     2281 caaggtcacc gacgcctgcg tgaactgcgg cgcgtgcgag cccgagtgcc cggtttccgc
     2341 catcagcgag aagaacggtg cgcgcgtcat cgacgccgcc acctgcatcg actgcggctc
     2401 ctgcgcttcc gtctgcccca ccgaggcgat cctcgcgcca taagccttcc ctatccagcc
     2461 gagccttccc cgctagggcc ctccgggacc cgcagggcgg aggctcacgg ggcccttcct
     2521 cgccgcgcgg cgatggaatg gccccttttt tatccggtgg ctttcgggta tagttggaaa
     2581 aatcgacgcc cgggagtccg agaatgagcg ggatacgaga cctcggtact ggcaatgtgg
     2641 cgcgtgccga aggcagggcc gccttcgacg cccgcggggg ccttgagagc ctcctcaccc
     2701 acctcgtctc cgaatcctac ctcctcctcc tcaggtcggt gaagtcgagc catgactcga
     2761 tgcgcaccat ctattcggag tggaacaggg gggagctggc cgaccccctg atcgccgcga
     2821 cggcggacgc cctcggcctg cgcgacctgg acggggagcc cctgctggag aaggtcctcg
     2881 acgtcgcctc gcgggccgag ccctgcgtca acgcggcggc cctggccatg gacctgggcg
     2941 tgcccgcccc cgccttcgcc caggcggccg cctcgagcca catctccgcc ctgaaggacg
     3001 agcgcgtcga cgccagcgcc gtcctctccg gccccaagca cgcgaccacg ggggacagga
     3061 cggccctggt cgaggagatc aggagggcgc ttctcgctgc caccatcctg gcccaggccc
     3121 aggccttcct cctcctctca cgcgccgcgg ccgcgaaggg cgcgttggcc gacctctgcg
     3181 gcaccgccca gctctggtcc gaaggccggg cttccttcct ccaccagcgc ctcggtgagg
     3241 ccctctgccg gaatcccgag ctcgagctcg tcatcctcga cgcccagatc aaggccctcc
     3301 tcgacccctg tctcccctcg ctgcgccgag tggtggcgcg cagcgccgag tcgggcctgg
     3361 cagtccccgt cttcgcagcc tgcctccagt tctacgacca gtgcagaagc acctggctgc
     3421 cggcgaacct ggtccgagcc ctccgcgacg cccgcggagg cgagggcttc gagcgcgtgg
     3481 acaggccacg gggggagatc ttccactcgg actggaagta gggcagcctc cctgcccaga
     3541 catcctcaag gagcgcagca tgccaaagcg aaccagcctc tccatggcgg ggctctgcct
     3601 cgcggtcgcc ctgctcgccg gcatggcggc ggtcttcggg gtcttctttc gcggcgacgg
     3661 ctcctcggcc acggcgataa gcgtccgcgg cgagcagttc gactacgcca caaacggggt
     3721 ctaccgatac aacgcccagc gcgtcgtcgc cgagggggtg ggctgggacg tcttcaccct
     3781 cttctgcgcc gtgcccgcca tgctcgcctg cctccccgcc ctggcccgcg gctccttccg
     3841 cgggaggctc ttcgccctgg gcctcctcgg ctatttcttc taccagtacc tcgagtactc
     3901 cctcacctgg gcctatgggc ccctcttcct tcccttcatc gccatctatg ccctgagcct
     3961 cgcgggcata gcctggatcg cgccaagcgt gggcctcgcc ggcctgtcca ggcgcttcgg
     4021 gccgggcttc ccccggaggg gcatggccgt gctctgcttt gcgatggctg cgatcctcct
     4081 cgtgatgtgg ctctccctca tcatcccgac gatggggggc agcgctgtcg gcctgctcaa
     4141 gggccagacc accctggtcg tccaggccct cgacctcggc ctcatcgtgc ccctctgcct
     4201 cttcaccggg atcgccgcct ggagggcaag accgattggc tacctcctct gctccgtctt
     4261 cgtcgtcaag gcc
//
LOCUS       DASSQV010000390         3516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1834502, whole genome shotgun sequence.
ACCESSION   DASSQV010000390 DASSQV010000000
VERSION     DASSQV010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834502"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="VFL04_06785"
     CDS             <1..1581
                     /locus_tag="VFL04_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7839449.1"
                     /translation="VLVLGEDVWNTAPILALGLRQAARVAPNRAASAKMRISPWDDAA
                     LREAVQNQKGPFFVATPEGGELDTFAKESYRGAPEDIARLGYAVAHAIDPAAPAPAGL
                     GAEAAALAQAIAAALVAAETPLVVSGSALGSPTLVEAAASVCRALKAAGRSPLISLVF
                     PEANTLGASLLGPRGLEAAAREMGEGRPLVVAEADLFRAGPELAAALLGKAKGLIVLD
                     HSQTATARAADLVLPVAALGEGSGSFVSSEGRAQRFFAVFKPEAPIAEAWRWLGLIAH
                     AAGRAAKAWDKLDEIHASIAAELPALARLSDAAPGGDWRFEGQRIGRGPHRESGRTAL
                     AAHIDVHEAEPPKDPDSGLAYTMEGEPRSQPSSLVPRFWAPGWNSDQSVNKFQVEVNG
                     ALYGGDPGARLFEPGEDRLGYAGLSAPPAFAPRKDALLALPRFHAFGSDELSSLAPAV
                     RQRIEEAHVAVSAKDAERLGLAPGARVRLGFVDGGETQVLRLVVRDMGEGLASLPVGF
                     EGVPALGGPAWVRIEAAE"
     gene            1578..2522
                     /gene="nuoH"
                     /locus_tag="VFL04_06790"
     CDS             1578..2522
                     /gene="nuoH"
                     /locus_tag="VFL04_06790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET7839450.1"
                     /translation="MSPTLFAIALVFGILLSAALVIWVERRLLAVFQERYGPNRVGPL
                     GLVQVVADIVKILTKENWVPPFADKPVFIIAPMIIMVTVLASFGVIPLGPGVVASDLN
                     VGLLFFLGMSSLAVYSVALAGWASNSKFALLGGMRAVGQMVSYEVFMGISLLGVVLQT
                     GSFSLVDIIEAQRGLWFCVPQFVGFVVFFIAGLAETRRIPFDIPEAESELTAGYHTEY
                     SGFKFAMFYLGEYVGVTLISAMVVVLFFGGWLGPWLPPFVWFALKTLFFVAVFILIRG
                     SLPRPRYDQLIGFGWKLMFPLALANLVATGGIVLALGR"
     gene            2533..3048
                     /gene="nuoI"
                     /locus_tag="VFL04_06795"
     CDS             2533..3048
                     /gene="nuoI"
                     /locus_tag="VFL04_06795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET7839451.1"
                     /translation="MVSILRAIWVVLKHLVSPKVTILYPEKKRRLPARWRGRIILSRD
                     PDGQERCVGCYLCAVACPPSCISLQAATAEDGRRYPSFFRINFSRCIFCGYCEEACPT
                     YAIQLTPDYEMGEYARKNLVYEKEDLLISGQGKYPGYNFYRAAGLSIKGKDKGEGENE
                     RPPVDVKDLLP"
     gene            3065..>3516
                     /locus_tag="VFL04_06800"
     CDS             3065..>3516
                     /locus_tag="VFL04_06800"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET7839452.1"
                     /translation="MNTAFVVAGLVAIVSTLMVIVQRNAVHALLYLIVSLFALAVTMF
                     LLGAPFVAALEVIVYAGAIMVLFVFVVMMIGAESSRVESRAWIGPSLLGLVLLAELAW
                     VLLLAPTAAAKVGAPVVGVSPKELGIALLGPYLVGVEIAAFLLLAGLVG"
BASE COUNT          478 a         1313 c         1139 g          586 t
ORIGIN      
        1 gtcctcgtcc tcggcgagga tgtctggaac acggccccca tcctcgccct cggcctcaga
       61 caggcggcca gggtcgcgcc gaaccgcgcg gcctcggcca agatgcggat ctccccctgg
      121 gacgatgccg ccctccgcga ggctgtccag aaccagaagg gccccttctt cgtggccacg
      181 cccgagggcg gcgagctgga cactttcgcc aaggaaagct acaggggggc gcccgaggac
      241 atcgcgaggc tcggctacgc cgtggcccac gccatcgacc ctgcggcccc cgccccagcg
      301 gggctcggcg ccgaggcggc agccctggcc caggccatag ccgccgccct cgtggccgcc
      361 gagacgcccc ttgtggtctc gggctcggcc ctgggcagcc cgaccctcgt cgaggccgcg
      421 gcctccgtgt gtcgggccct caaggccgcc ggccgctcgc ccctcataag cctggtcttc
      481 cccgaggcca acaccctcgg agccagcctc ctcggaccac gcggcctcga ggccgctgcg
      541 cgtgaaatgg gggaggggag gcccctcgtc gtcgccgagg ccgatctctt ccgcgccggg
      601 cccgagctcg ccgcagccct cctgggcaag gcgaagggcc taatcgtcct cgaccacagc
      661 cagacagcga cggccagggc cgccgacctc gtcctgcccg tggccgccct gggcgagggc
      721 tcgggcagct tcgtctcgag cgagggcagg gcccagcgct tcttcgcggt cttcaaaccc
      781 gaggcgccca tcgccgaggc ctggcgctgg ctcggcctta tcgcccatgc cgccggccgc
      841 gccgccaagg cctgggacaa gctcgacgaa atccacgcca gcatcgccgc cgagctgccg
      901 gccctggcga ggctcagcga cgcagccccc ggcggggact ggcgcttcga gggccagagg
      961 atcgggcgcg gcccccaccg cgagagcgga cgcacggccc tggccgccca catagacgtg
     1021 cacgaggccg agccgcccaa ggaccccgac tcgggcctcg cctacaccat ggagggcgag
     1081 ccgaggtccc agccctcctc cctcgtgccg aggttctggg ccccgggctg gaactccgac
     1141 cagtcggtca acaagttcca ggtcgaggtc aacggcgccc tctacggcgg cgaccccgga
     1201 gccaggctct tcgagcctgg cgaggacagg ctcggctacg ctggcctctc ggccccgccg
     1261 gccttcgccc cgcgcaagga cgccctcctt gccctgcccc gtttccacgc ctttggctcg
     1321 gacgagctct ccagtctcgc cccggcggtg cggcagagga tcgaggaggc ccatgtcgcg
     1381 gtctcggcga aggacgcaga gcgcctcggc ctcgccccag gagccagggt gaggctcggc
     1441 ttcgtcgacg gcggggagac ccaggtcctg aggctcgttg tgcgcgacat gggcgagggc
     1501 ctcgcctcgc tgccagtggg cttcgagggc gtccccgccc tcggcggccc cgcctgggtc
     1561 aggatcgagg cggccgaatg agcccgaccc tcttcgccat agccctggtc ttcggcatcc
     1621 tcctctcggc ggccctcgtg atctgggtcg agaggcggct cctcgccgtc ttccaggagc
     1681 gctacggccc caaccgcgtc gggcccctcg gcctcgtcca ggtcgtggcc gacatcgtca
     1741 agatcctcac gaaggagaac tgggtaccgc ccttcgccga caagcccgtc ttcatcatag
     1801 ctccgatgat catcatggtg acggtgctcg cctccttcgg cgtgatcccc ctggggcccg
     1861 gcgtggtcgc ctcggacctc aacgtgggcc tcctcttctt cctcggcatg tcctccctcg
     1921 cggtctacag cgtcgccctc gccggctggg cctcgaactc caagttcgcc ctcctcggcg
     1981 gcatgagggc cgtgggccag atggtgagct acgaggtctt catggggatc tccctcctcg
     2041 gcgtcgtcct gcagacgggc tccttcagcc tggtggacat catcgaggcc cagaggggcc
     2101 tctggttctg cgtgccccag ttcgtgggct tcgtcgtttt cttcatcgcc ggcctcgcgg
     2161 agacgaggcg gatccccttc gacatccccg aggccgagag cgagctcacc gccggctacc
     2221 acacggagta ctcgggcttc aagttcgcca tgttctacct tggcgagtac gtcggcgtga
     2281 ccctgatctc ggccatggtc gtcgtcctct tcttcggcgg atggctgggg ccttggctcc
     2341 cgcccttcgt ctggttcgcg ctcaagaccc tgttcttcgt cgccgtgttc atcctcataa
     2401 ggggctcgct gccgcggccc cgctacgacc aactcatcgg tttcggctgg aagctcatgt
     2461 tccccctcgc gctcgcgaac ctcgtcgcga cggggggcat cgtgctcgcg ctggggcggt
     2521 aaggagagac ccatggtgag catcctgagg gcaatatggg tcgtgctgaa gcacctcgtc
     2581 agccccaagg tgacgatcct ctatcccgag aagaagcggc gcctgccggc gaggtggcgc
     2641 gggaggatca tcctctcccg cgaccccgac ggccaggagc gctgcgtcgg ctgctatctc
     2701 tgcgccgtgg cctgcccgcc ctcctgcatc tcgctgcagg cggcgaccgc agaggacggg
     2761 aggcgctacc cctccttctt ccgcatcaac ttctcgcgct gcatcttctg cggctactgc
     2821 gaggaggcct gtccgaccta cgcgatccag ctcacccccg actacgagat gggcgagtac
     2881 gccaggaaga acctcgtgta cgagaaggag gacctcctca tctcgggcca gggcaagtac
     2941 cctggctaca acttctaccg ggcggcgggg ctctcgatca agggcaagga caagggcgag
     3001 ggcgagaacg agagaccgcc ggtcgacgtg aaggacctcc tgccatgacc gaaaggaagc
     3061 acgcatgaac accgccttcg tcgtcgccgg cctggtcgcg atcgtctcga ccctcatggt
     3121 catagtgcag aggaacgccg tccacgccct gctctacctc atcgtgtccc tcttcgccct
     3181 cgcggtgacg atgttcctcc ttggcgcccc cttcgtcgcc gccctcgagg tgatcgtcta
     3241 cgcgggagcg atcatggtgc tcttcgtctt cgtggtcatg atgatcgggg ccgagagctc
     3301 gagggtggag agccgggcct ggatcggtcc gtccctcctc ggcctcgtcc tcctcgccga
     3361 gctcgcctgg gtcctcctcc tcgcgccgac ggcggccgcg aaggtgggag cgcccgtcgt
     3421 cggggtgtcg cccaaggagc tcggcatcgc cctgctcggc ccctacctcg tgggcgtcga
     3481 gatcgccgcc ttcctcctcc tcgcgggtct ggtcgg
//
LOCUS       DASSQV010000391         3916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1459350, whole genome shotgun sequence.
ACCESSION   DASSQV010000391 DASSQV010000000
VERSION     DASSQV010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459350"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFL04_06805"
     CDS             <1..449
                     /locus_tag="VFL04_06805"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET7839453.1"
                     /translation="AARRAGAGAPRLVLAADTLVCVSDRGSETALGKPRDLAEARSMI
                     ALLSGRSHQVHTGLALLDRWSGRLETVRSDSTVHFAKMEAEEIEAYLDTGEWRGVAGA
                     YRVQGAAALHIARIEGSWSGIVGLPIHELYVILRAADYRIRPRSPG"
     gene            633..1514
                     /gene="rpsB"
                     /locus_tag="VFL04_06810"
     CDS             633..1514
                     /gene="rpsB"
                     /locus_tag="VFL04_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET7839454.1"
                     /translation="MAVVTMKNLLESGVHFGHQVKRWDPRMKKYIFAERNGIHIIDLQ
                     KTIQAIKEAYDAVQKTVASGKTVLFVGTKKQAQTAIQKEAERCEQFWVNNRWLGGMLT
                     NFSTIKKSIQRLKKIEKMEVDGTFESLTKKEIASLQKEKSKLEKNLGGIKEMKEHPGV
                     LFVIDTRKEAIAVAEAQRLGIPIIAVVDTNCNPEGITYPIPGNDDAIRSISLFTQIIA
                     NAVVEAGAAEGLKIIENLQGDETEEIKTDAAPPSDEDEVEIDPATYSAADKPKAEEAT
                     TEEPAPIVDEDRLYEAK"
     gene            1539..2390
                     /gene="tsf"
                     /locus_tag="VFL04_06815"
     CDS             1539..2390
                     /gene="tsf"
                     /locus_tag="VFL04_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET7839455.1"
                     /translation="MDIKASDVKNLREKTGAGMMDCKKALVEANGDIAAAEKILREWG
                     MAGVEKRAGRATNEGRIFVKEAEGKVTAVEIACETDFVSRNKDFIEAGEKIVAHAYGK
                     SANGASPELEGMVKDIAVLIKENITLKRVRTVAAAAGEYVHSYSHGEGKIVVVVKAKS
                     DKAEAFKNEKVQAFVHDIALHIAAFNPMFLDQTKPDAAWVKEQQEIFQKQIELDEKMK
                     GKPANVIEGILKGKLKKLMSEICLLDQGFVKDEKVPVAAAMAAVAKEAGCKLELADYV
                     YLKVGEA"
     gene            2534..3088
                     /gene="frr"
                     /locus_tag="VFL04_06820"
     CDS             2534..3088
                     /gene="frr"
                     /locus_tag="VFL04_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET7839456.1"
                     /translation="MDSVKSACEERMKKAVVALKDEFNGLRTGRASPSLLDKIRVDYY
                     GQKTPLSQVATVSVPEARLIVIQPWDRSLIGEIEKGIQKSELSLNPSNDGKVIRIAIP
                     PLTEERRKDLAKMARSMAEQARVSVRNVRRDGLEDLKKQLAAGHVPEDAIKKEEDELQ
                     KLTDSYIAQIGKTLEEKEKEIMEV"
     gene            3104..3805
                     /gene="uppS"
                     /locus_tag="VFL04_06825"
     CDS             3104..3805
                     /gene="uppS"
                     /locus_tag="VFL04_06825"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET7839457.1"
                     /translation="MPHNASSSQGPVHVGIIMDGNGRWASARGLPRTAGHREGLDVAK
                     RIVRAAEDLGIRVLSLYTFSTENWKRTTEEVGFLMGLIRRHLVAELDFYRANGIRVVH
                     SGNLAGLPPEIVLELDRVQAETRDFDRMTVNLAINYGGRDEIVRALGRLAASGRAVDE
                     ASLSSAMDRPELPDPDLIIRTAGEMRLSNFLLWESAYAELWFSQKLWPDFGPEDIALA
                     VEDYRRRERKFGGIR"
BASE COUNT          837 a         1244 c         1269 g          566 t
ORIGIN      
        1 cggcggctcg gcgcgccggg gcaggcgctc cacggctggt gctggccgcc gataccctgg
       61 tatgcgtgtc cgatcgggga tccgagaccg ccctcggcaa accgcgcgac ctcgcggagg
      121 cgcggtccat gatagccctc ctctccggac gcagccatca ggtgcacacg ggactcgccc
      181 tgctcgaccg gtggagcggg cgcctcgaga cggtgaggtc ggactcgact gtccacttcg
      241 cgaagatgga ggcggaggag atcgaggcct acctcgacac cggagaatgg cggggggtgg
      301 ccggcgcata ccgggtccag ggggccgccg ccctccacat cgcccgcatc gagggctcct
      361 ggtcgggcat cgtgggcttg ccaatacacg agctttatgt catcttacgc gctgcggatt
      421 accgcatccg gcctcgctcg ccaggctgac cccaaggggt ctggcggcgt ccggatcggg
      481 cggaccgggc catcggctcc ggatcatgcc caccgacgcc ttcggcgtcg gcaccttcca
      541 tcatccacga aggccagaag cgattctgga ccgggcggac gatgagatac agagacggga
      601 ggccggcttt ggaccggcca cgggaggacc tcttggcagt cgttacgatg aagaacctgc
      661 tcgagtctgg tgtgcatttc ggacaccagg tgaaacgatg ggacccgcgc atgaagaagt
      721 acatcttcgc tgagcgcaac gggatccaca tcatcgatct ccagaagacc atccaggcga
      781 tcaaggaagc ctatgacgcg gtccagaaga ccgtggcctc gggcaagacg gtcctcttcg
      841 tcgggacgaa gaagcaggcc cagaccgcca tccagaagga agccgagcgc tgcgagcagt
      901 tctgggtcaa caaccgttgg ctcggcggca tgctcaccaa cttctccacc atcaagaagt
      961 cgatccagcg cctcaagaag atagagaaga tggaagtcga cggcacattc gagagcctga
     1021 ccaagaagga gatcgcgagt ctccagaagg agaagtccaa gctcgagaag aacctcggcg
     1081 gcatcaagga gatgaaggag caccccggcg tgctcttcgt catcgacacc cgcaaggaag
     1141 ccatcgccgt cgccgaggcc cagcgcctcg ggatcccgat catcgccgtc gtcgacacca
     1201 actgcaaccc cgagggcatc acctacccca tccccggcaa tgacgacgcc atccgctcga
     1261 tcagcctctt cacccagatc atcgcgaacg cggtggtcga ggccggagcc gccgagggcc
     1321 tgaagatcat cgagaacctc cagggcgacg agaccgagga gatcaagacc gacgccgccc
     1381 cgccctccga cgaggacgag gtggagatcg accccgccac ctacagcgcc gccgacaagc
     1441 cgaaggccga ggaggccacg accgaggagc cggctcccat cgtcgacgag gaccgcctct
     1501 acgaggccaa gtaagacaac caggtcagga gaaagaacgt ggacatcaag gcaagcgacg
     1561 taaagaacct ccgcgagaag accggcgcgg ggatgatgga ctgcaagaag gcccttgtcg
     1621 aggcgaacgg cgacatcgcc gccgccgaga agatcctccg cgagtggggc atggccggcg
     1681 tcgagaagcg cgccggacgg gccaccaacg agggccgcat cttcgtcaag gaggccgagg
     1741 gcaaggtcac cgcggtcgag attgcctgcg agacggactt cgtgtcgcgc aacaaggatt
     1801 tcatcgaggc cggcgagaag atcgtggccc atgcctatgg caagagcgcc aacggcgcca
     1861 gccccgaact cgaggggatg gtcaaggaca tcgccgtcct catcaaggag aacatcacgc
     1921 tcaagagggt gcgcaccgta gcggctgcgg ccggcgagta cgtgcactcc tattcccacg
     1981 gcgagggcaa gatcgtcgtc gtcgtgaagg cgaagtccga caaggccgag gccttcaaga
     2041 acgagaaggt ccaggccttc gtccatgaca tcgccctcca tatcgcggcc ttcaatccca
     2101 tgttccttga tcagacaaag cccgacgccg cctgggtcaa ggagcagcag gagatcttcc
     2161 agaagcagat cgagctcgac gagaagatga agggcaagcc cgcgaacgtc atcgagggca
     2221 tcctcaaggg caagctcaag aagctcatgt ccgagatctg cctcctcgac cagggcttcg
     2281 tgaaggacga gaaggtgccg gtcgcagccg ccatggctgc ggtggccaag gaggccggct
     2341 gcaagctcga gctggctgac tatgtctatc tgaaggtcgg agaggcctga tgagccaaac
     2401 gggccagggc ccgttggcgt gagtgcgcgg attccgccgc agacgcggcg gaatccgcgt
     2461 ctttcgtttg ggcgcagctg cacggacccg cgagagaccg ggcacaacgc gttcccatcc
     2521 aggaggggag acaatggatt cggtgaagag cgcctgcgaa gagcggatga agaaagcggt
     2581 cgtcgcactc aaggacgagt tcaacggcct caggacgggc cgggcctccc cgagccttct
     2641 cgacaagatc cgcgtcgact actacggcca gaagacgccc ttgtcccagg tcgcgaccgt
     2701 ctccgtcccc gaagcccgcc tcatcgtcat ccagccctgg gaccggtcac tcatcggcga
     2761 gatcgagaag ggcatccaga aatccgagct ctccctcaat ccgtccaacg acggcaaggt
     2821 gatccgcatc gcgatccccc cgctaaccga ggagcgccgg aaggacctcg cgaagatggc
     2881 gaggtcgatg gccgagcagg cgcgggtctc ggtgcgcaac gtgcgccgcg atgggctcga
     2941 ggacctcaag aagcagctcg cggccggcca tgtcccggag gacgcgatca agaaggagga
     3001 ggacgagctg cagaagctca ccgactccta cattgcgcag atcgggaaga cgctcgagga
     3061 aaaagagaaa gagataatgg aagtctgata aagggagcca atcgtgccgc ataacgcgtc
     3121 ctcgagccag ggtcctgtcc atgtcggcat aatcatggac gggaacgggc gatgggcgag
     3181 tgcccgtggg ctgccacgca ccgccggaca ccgtgagggg ctggacgtcg ccaagcggat
     3241 cgtccgggcg gccgaggacc tggggatccg ggtcctgtcc ctctacacat tctccaccga
     3301 gaactggaag cgcaccaccg aagaggtggg tttcctgatg ggcctcatac gacggcacct
     3361 cgtcgccgaa ctcgatttct acagggccaa cggtatcagg gtggtccatt ccggcaacct
     3421 cgccggcctg ccgcccgaga tcgtcctgga gctggacagg gtccaggccg agaccaggga
     3481 tttcgacaga atgacggtga acctggccat caattacggg ggccgggatg agatcgtgag
     3541 ggccctaggc cggctggcgg cctcggggag ggcagtcgac gaagcgagcc tttcctcggc
     3601 gatggacaga cccgagctgc ccgatccaga cctcataata aggacggccg gcgagatgcg
     3661 gctgtcgaac ttcctgctct gggagtcggc ctatgccgag ctctggttca gccagaagct
     3721 ctggcccgat ttcggccccg aggacatcgc ccttgccgtc gaggactacc gccggcgcga
     3781 gcggaaattc ggcggcatca ggtgagcaac ctcatccaga gactcctgct cttcaccctc
     3841 ggcatcccgg cgatgcttgc gatcgtcatc ctcctcccag ccgcccatca cgccgccggt
     3901 gtcgcgatca tcctgg
//
LOCUS       DASSQV010000392         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2456687, whole genome shotgun sequence.
ACCESSION   DASSQV010000392 DASSQV010000000
VERSION     DASSQV010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2456687"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VFL04_06830"
     CDS             <1..822
                     /locus_tag="VFL04_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET7839458.1"
                     /translation="RIVGTVVKDPVVLRVLVRGDFPSLLRGQSFGALERHGPFLRFAL
                     EAEPPQVPLDLVMHLMLAGRLRLAEPGEKALPATAFSLELDDGLTLHYGDEKSMGKVY
                     LGERGRFEAIPGYTSQGVDITTEAFTWEAFSALIAKKRCQARVFIMDQGCLSAIGNAY
                     ADEILFEAGIHPKAACSSLSPEERRRLYDAVLKVIAWGIEEVEKAGRPIEDKVRGHMR
                     VRNRAGEACPRCGSTIRKAGVLGFDSFFCPRCQVDRAGKGIVDWTKLPSGTERKP"
     gene            819..1220
                     /locus_tag="VFL04_06835"
     CDS             819..1220
                     /locus_tag="VFL04_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HET7839459.1"
                     /translation="MSDLWFVMLGGAFGSGMRWLVSRASARFLGSSFPWGTHIVNLVG
                     CLLIGFTLGLVEHGIGGRRLRPLAVVGFLGGLTTFSSFAFETVDLARRGEWLKAGANF
                     ALDNLGGMALAAFGLALGIAVAASVRGGRPL"
     gene            complement(1286..1561)
                     /locus_tag="VFL04_06840"
     CDS             complement(1286..1561)
                     /locus_tag="VFL04_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839460.1"
                     /translation="MSPALALGIGALAAAPLAAILALRYPGLRRCLGPPGRRAYALRS
                     VCLVLASGSASGLLASGAGGLAWPIVGELALSLAAALLILGLLRRGE"
     gene            complement(1558..2844)
                     /locus_tag="VFL04_06845"
     CDS             complement(1558..2844)
                     /locus_tag="VFL04_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7839461.1"
                     /translation="MSRLRAWATAASLLLVAAGILFVEPLSFVLALPLAVWLLLGVVV
                     GSPEPRLEATRTASSSFPEPGTRGEMRLAVRHLGPALACLRLSDPILPGMRIVEGEPT
                     WAGPLEAGAQAEIAYAAVFERGAYEFEAIEARAEHPLTAMETRIALACPATLVAPPPP
                     LAPPRSAFRSMHPRPFAGASRLRRQGQGTEFSGTREYAPGDPLRSLNWRAEALWDQGI
                     VNVFEEERAIDVELILDARSEAYEDLALFEAAAAAASAMAERFLDDGDRVAFLCYGSA
                     IEWIAPGSGREQRLRIRRSAGKARLGAHPVFERFDSLPVGLFPPGSLVLLVSPLLGAD
                     LLALRSLAALGYRVAVLRPDPLGKGGQGQGQGGLVPEPGARTQGQGQAQSLARRFCAL
                     EAEILMSRLLRAGIEILDWNPSRPLASAVLHGGSRP"
     gene            complement(2848..>3100)
                     /locus_tag="VFL04_06850"
     CDS             complement(2848..>3100)
                     /locus_tag="VFL04_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7839462.1"
                     /translation="TRTAARTAVGASPRASLAMLKLSRARALMEGRSFVLPDDLKWCA
                     FEVLIHRVLLKPEVWAAETRVADVVQQAIDAVPVPKTEA"
BASE COUNT          470 a         1065 c         1137 g          428 t
ORIGIN      
        1 aggatcgtgg ggactgtcgt caaggacccg gtcgtccttc gggtcctcgt gcgcggggat
       61 ttcccctccc tcctccgggg ccagagcttc ggcgccctcg agcgccacgg ccccttcctg
      121 cgcttcgccc tcgaggcaga gcctccccag gttcccctcg accttgtcat gcacctcatg
      181 ctggccggga ggctcaggct ggccgagccg ggggagaagg ccctccccgc gacggccttc
      241 agcctcgagc tcgacgacgg tctgaccctc cactacggcg acgaaaagag catgggcaag
      301 gtctatctgg gcgagcgggg ccgcttcgag gcgatcccgg gctacacgag ccagggcgtc
      361 gacatcacca cggaggcctt cacctgggag gccttctcgg ccctcatcgc caagaaacgc
      421 tgccaggcga gggtcttcat catggaccag ggctgtcttt cggcgatcgg gaacgcctat
      481 gccgacgaga tcctcttcga ggcagggatc caccccaagg ccgcttgctc cagtctctcg
      541 cccgaggaga ggcgccgcct ctacgatgcc gtcctcaagg tcatagcctg ggggatcgag
      601 gaggtcgaga aggccggccg ccccatcgag gacaaggtga gggggcacat gagggtccgc
      661 aaccgggccg gcgaggcctg tccccgctgc ggctcgacca taaggaaggc cggggtcctc
      721 ggcttcgact cctttttctg ccccagatgc caggtcgacc gcgccggcaa gggcatcgtc
      781 gactggacca agctgccatc ggggacggaa aggaagccat gagcgacctc tggtttgtga
      841 tgctgggcgg ggccttcggc tcggggatgc gctggctcgt gtcgagggca tcggcccgtt
      901 tcctcggcag ctccttcccc tgggggaccc acatcgtcaa cctcgtgggc tgtctcctca
      961 taggctttac cctgggcctc gtcgagcacg ggatcggcgg ccgaaggctc cgccccctcg
     1021 ccgtcgtcgg cttcctcggc gggctcacca ccttctcgtc cttcgccttc gagaccgtcg
     1081 acctcgcccg gaggggggaa tggctcaagg caggggcgaa cttcgccctg gacaacctcg
     1141 gcggcatggc cctggcggcc ttcggcctcg ccctcggcat agccgtcgcg gcctcggtca
     1201 gggggggccg gcccttgtag cggcccgatc cctgcggcga ctttacgaag accgcggccg
     1261 agcggagccc agacagcccc aggcctcact cgccgcggcg cagaagtcca aggatgagga
     1321 gggccgcggc caggctcagg gccagctccc cgactatggg ccaggcgagg ccaccggccc
     1381 ccgaggcgag cagaccgctc gcggagccac tggccaggac gaggcagacc gagcggaggg
     1441 cgtaggccct gcgccccggc ggtccgaggc agcggcgcag acccgggtat ctgagcgcga
     1501 ggatggcggc gaggggggct gcggccaggg ccccgatgcc gagggcgagg gctgggctca
     1561 tgggcgagag cctccgtgga ggacggccga ggccaagggc cgcgaagggt tccagtccag
     1621 gatctcgatc cccgcgcgca ggagccggct catgaggatc tcggcctcga gggcgcagaa
     1681 cctgcgggcg agggactggg cctggccttg cccctgggtc cgggccccgg gctcaggaac
     1741 cagcccgccc tgaccctgac cctggccccc cttcccgagg gggtcgggcc tcaggacggc
     1801 caccctgtag ccgagggcgg ccagactccg cagggccaaa aggtcggcgc caagaagggg
     1861 gctcacgagg aggacgaggg agcccggcgg gaaaaggccg acggggaggc tgtcgaagcg
     1921 ctcgaagacg gggtgggccc cgagcctggc cttgcccgcc gacctcctga tcctgagcct
     1981 ctgctccctc cccgagccgg gggcgatcca ctcgatggcc gagccatagc agaggaaggc
     2041 caccctgtcc ccgtcgtcga ggaagcgttc ggccatcgcc gaggcagcgg cggccgcggc
     2101 ctcgaaaagc gcgaggtcct cgtaggcctc ggagcgcgcg tcgaggatga gctcgacatc
     2161 gatggcccgc tcctcctcga agacgttgac gatgccctgg tcccagaggg cctcggccct
     2221 ccagttgagg ctccgcaggg ggtcgcccgg ggcatactcc ctcgtgcccg agaactcagt
     2281 gccttggccc tgtctccgca gccggctggc gcccgcgaag ggcctcggat gcattgagcg
     2341 gaaggccgaa cggggcgggg cgaggggagg gggtggtgcg accagggtcg ccgggcaggc
     2401 cagggcgatc ctggtctcca tcgccgtgag cggatgctcg gcccgggcct caatggcctc
     2461 gaactcgtag gctccgcgct cgaagacggc ggcgtaggcg atctcggcct gagcgcctgc
     2521 ctccaggggc cctgcccagg taggctcccc ctcgacgatc ctcatcccgg ggaggatggg
     2581 gtcgctgagc ctgaggcagg ccagggccgg gccgaggtgc cgcacggcga ggcgcatctc
     2641 gcccctcgtg ccaggctcgg ggaagctcga ggaggccgtc cgcgtcgcct cgagcctggg
     2701 ctcggggctc ccgaccacga ccccgaggag gagccagacc gcgagcggca gggcgaggac
     2761 aaacgagagg ggctcgacga acaggatccc agcggcgacg aggaggaggc tcgctgcagt
     2821 cgcccaagcc cgcaggcggc tcaaggccta ggcctcggtc ttggggacgg ggacggcgtc
     2881 gatggcctgc tggaccacgt cggcgaccct ggtctcggcc gcccagacct cgggtttgag
     2941 gaggacgcgg tggatcagga cctcgaaggc gcaccatttg aggtcgtcgg gcaggacgaa
     3001 ggagcggcct tccatgaggg ctcgggcccg ggagagcttg agcatggcga gggaggcccg
     3061 ggggctcgct ccgacggcag tcctggccgc cgtcctcgtg
//
LOCUS       DASSQV010000393         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_218128, whole genome shotgun sequence.
ACCESSION   DASSQV010000393 DASSQV010000000
VERSION     DASSQV010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218128"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFL04_06855"
     CDS             <1..348
                     /locus_tag="VFL04_06855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET7839463.1"
                     /translation="LKAEGWRLIQILAVSSAEANELSYSFGLGMQFKVLRFKVASDQT
                     VPSITPVYLAAYLYENEIRDLFGVKIERIAVDWLGKVYDVKGDKPFSRVTVAGSCVEC
                     PPSSITAHQGTKS"
     gene            345..1448
                     /locus_tag="VFL04_06860"
     CDS             345..1448
                     /locus_tag="VFL04_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HET7839464.1"
                     /translation="MSAHEKAAPGPTVIPFGPQHPVLPEPLHFDLVLQDEKVVGAIPQ
                     IGYIHRGLELLVDKRDYLDYVHVAERICGICSFMHGMGYCEAVETVLGITVPDRARWL
                     RTFWCELARVHSHLLWLGCGADALGFENLFMASWRARETVVDVAEETAGGRVIFGSAL
                     VGGVSKDPSDETLKRCVAAMKGVRAETLRLANIVMNESTVRHRFEGVGVLSKEMAYAL
                     GAVGPMLRASGVDYDVRRRGYAAYGEVDFETVVETGGDCMARALVRVREIEQSFHIID
                     QIVATMPAGPVAVPVKGMPPVGEAWVRLEQPRGEVLYYVRSNGTRNLERFRVRTPTFA
                     NVPAMVEAVKGASLADVPNIILTIDPCISCTER"
     gene            1469..1864
                     /locus_tag="VFL04_06865"
     CDS             1469..1864
                     /locus_tag="VFL04_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7839465.1"
                     /translation="MARTALRNFFTRPATRRYPFVVRPVFEATRGRIVNDFPKCILCS
                     MCAKHCPANAIVVDKATGIWKIDRFACVICGACVRVCPVKCLAMSNERAKAIDFSDIP
                     GRSHEHNRATEPAAAKPAAPKPAGEAPNA"
     gene            1857..>2246
                     /locus_tag="VFL04_06870"
     CDS             1857..>2246
                     /locus_tag="VFL04_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HET7839466.1"
                     /translation="MHEASAVEAVVRIVDQEVRRMDAASAAGAPLGAPSAKPRVAKVH
                     LVVGEGTGHMEASLVFYFGVLAKGTRAEGAELVVSYVKPRLRCQSCGESFERKRFSFD
                     CPTCGGPGEMTRIGSEFYVDTIELENGG"
BASE COUNT          365 a          830 c          704 g          347 t
ORIGIN      
        1 ctcaaggccg agggctggag gctcatccag atcctcgccg tctcctcggc cgaggccaac
       61 gagctctcct actccttcgg gctcggcatg cagttcaagg tgctcaggtt caaggtcgcg
      121 agcgaccaga ccgtcccgag catcacgccg gtgtacctcg ccgcctacct ctacgagaac
      181 gagatccgcg acctcttcgg cgtcaagatc gagcgcatcg cggtcgactg gctcggcaag
      241 gtctacgacg tcaagggcga caagcccttc agcagggtca ccgtcgcggg cagctgcgtc
      301 gagtgccccc cctcctccat cacggcccat caaggaacga agtcatgagc gcgcacgaga
      361 aagccgcacc cggccccacc gtcatcccct tcgggcccca gcacccggtc ctgcccgaac
      421 ccctccactt cgacctcgtc ctccaggacg agaaggtcgt cggcgcgatc ccccagatcg
      481 gctacatcca ccgcggcctt gagctcctcg tggacaaacg cgactacctc gactacgtcc
      541 atgtggccga gcgcatctgc ggcatctgct ccttcatgca cggcatgggc tactgcgagg
      601 cggtcgagac cgtcctcggc atcaccgtgc ccgacagggc ccgctggctc cgcaccttct
      661 ggtgcgagct cgcgcgcgtc cacagccacc tcctctggct cggctgcggt gccgacgccc
      721 tcggcttcga gaacctgttc atggcctcat ggcgcgctcg cgagaccgtg gtcgacgtcg
      781 ccgaggagac cgcgggcggc cgcgtcatct tcggctcggc cctcgttggc ggcgtcagca
      841 aggaccccag cgacgagacc ctgaagcgct gcgtcgcggc gatgaagggc gtgcgcgccg
      901 agaccctccg cctggccaac atcgtgatga acgagagcac cgtgcggcac cgcttcgagg
      961 gcgtcggcgt cctttccaag gagatggcct acgccctcgg cgccgtcggc cccatgctcc
     1021 gcgcgagcgg ggttgactac gacgtgcgcc gccgcggcta cgccgcctac ggcgaggtcg
     1081 acttcgagac ggtcgtcgag accggcgggg actgcatggc ccgcgccctg gtccgcgtcc
     1141 gcgagatcga gcagagcttc cacatcatcg accagatcgt cgcgacgatg cccgccggcc
     1201 ccgtcgccgt gcccgtcaag ggcatgccgc ccgtcgggga ggcctgggtc aggctcgagc
     1261 agccgcgcgg tgaggtcctc tactacgtcc gctccaacgg cacccgcaac ctcgagcgct
     1321 tccgcgtccg caccccgacc ttcgccaacg tcccggcgat ggtggaggcc gtcaagggcg
     1381 cctcgctcgc cgacgtcccg aacatcatcc tcaccatcga tccgtgcatc tcctgcaccg
     1441 agaggtaatt cctgtggctt tactaccaat ggcgcggaca gcgctccgca acttcttcac
     1501 gaggccggct actcggcgct atcccttcgt cgtcaggccc gtgttcgagg cgacgagggg
     1561 acgcatcgtc aacgacttcc ccaaatgcat actctgctcg atgtgcgcga agcactgccc
     1621 cgccaacgcg attgtcgttg acaaggccac cggcatctgg aagatcgacc gcttcgcctg
     1681 cgtcatctgc ggcgcctgcg tgagggtctg ccccgtgaag tgcctcgcga tgagcaacga
     1741 gagggccaag gccattgact tctccgacat ccccggccgg agccacgagc acaaccgcgc
     1801 gaccgagccg gccgcggcca agcccgctgc ccccaagccc gcgggagagg ccccgaatgc
     1861 atgaggccag cgccgtcgag gccgtcgtca ggatcgtgga ccaggaggtc cgcaggatgg
     1921 acgcggcctc ggccgcgggg gccccgctcg gggcccccag cgccaagccc cgtgtggcca
     1981 aggtccacct cgtcgtaggc gaggggaccg gccacatgga ggcctccctc gtcttctact
     2041 tcggcgtcct cgccaagggg acgagggccg agggcgccga gctcgttgtg agctacgtga
     2101 agccccggct ccgctgccaa agctgcggcg agagcttcga gcgcaaacgc ttttccttcg
     2161 actgcccaac atgcggcggc cccggggaga tgacccggat cggctccgag ttctacgtcg
     2221 acacgatcga gctggagaac gggggc
//
LOCUS       DASSQV010000394         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2332087, whole genome shotgun sequence.
ACCESSION   DASSQV010000394 DASSQV010000000
VERSION     DASSQV010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2332087"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFL04_06875"
     CDS             complement(<1..668)
                     /locus_tag="VFL04_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7839467.1"
                     /translation="MNTLGIAFRNLGRNRRRSFLAILSVFMSMMIVIFADGFISGVLD
                     SIVRNATKNQTGHVNIATEDYRRRERFMPTNAAIGDADAVVAAIEARPELKGLVAQVA
                     PRVRFGVVLSSGTATKAAIGIAGESERERSLLMLDRSVLPGGSYLGAPGTAIVGSRLA
                     ADLGLKVGDSLKVVAQKADYGLGFKKFRIAGLFRTGMELFDDSAFQVGIDDARELLGL
                     GRGAS"
     gene            complement(680..1936)
                     /locus_tag="VFL04_06880"
     CDS             complement(680..1936)
                     /locus_tag="VFL04_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7839468.1"
                     /translation="MGTLWKIALRNTVRHRRRTIITAIVMTAGISVFIAFDSMLSGMD
                     RMAVDNLAQYTVSSLKVRNPAYVEDIQASPLDKPLSDPEAALRAIRAEGLSATARIRF
                     IAKLSNYTDEIPLLADAVDPRTDASVFALARSISAGSWLDGAASKSVVLGAALADELK
                     IKVGDSVLVSAQTVNDTTNADEYSVVGLVDTPAPEVNRSGLFMPMAAARELLAAPETR
                     GGFATEVDAALPREPTLNTTLAEGEKAAAALRAQLPGARVDPISFLARDYLSVRTAKA
                     KYSFVLVFVVLIIAAVGIVNTILMSVYSRVREIGVLRAYGMTPKDIARLFTLEGLALG
                     LVGSALGVGLGALLDWLLISQGINLDPYADAMGSLPLSGVLRGEWNLTTMAFGFVFGV
                     VVSLVAARIPARRAAKLEPTDALRFV"
     gene            complement(1938..2624)
                     /locus_tag="VFL04_06885"
     CDS             complement(1938..2624)
                     /locus_tag="VFL04_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7839469.1"
                     /translation="MAALHLEKVTKVYRKGESEVRALDGVDLDIEAGEFLTLVGPSGS
                     GKTTFLNILGCLDSPTEGTIVYDGTELHALGEKGLSAYRRERISFVFQSYNLIPVLTV
                     RENVELSLVIEKRLTPKEIGERAREMIAAVGLAGKEERFPRELSGGQEQRVAIARALV
                     KRPLVVLADEPTANLDSHTAEEIVELMRRINQERQTTFVFSTHDRLVMEHARRIVTIR
                     DGRVTGDERK"
     gene            complement(2626..>3015)
                     /locus_tag="VFL04_06890"
     CDS             complement(2626..>3015)
                     /locus_tag="VFL04_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7839470.1"
                     /translation="PASEPGGPRAGIRKLDLDRVIHERARLMVLTYLASSEKAEAGFT
                     ELKDELGFSSGNLSVQLKTLEEAGYVKIEKRFVKNKPYTGVSLTVDGQNALSAYLAEL
                     EVIVASLKGPRQTEGDAGAQARAGEED"
BASE COUNT          453 a          994 c         1058 g          510 t
ORIGIN      
        1 gaggcccccc tgccaaggcc cagaagctcg cgggcgtcgt cgatgccgac ctggaaggcc
       61 gagtcgtcga agagctccat ccccgtccgg aagaggccgg cgatgcggaa cttcttgaac
      121 ccgaggccgt agtcggcctt ctgggccacg accttgaggc tgtcgcccac cttgagcccc
      181 aggtccgccg cgagccggga gcccacgatc gccgtgccgg gggcgccgag atagctcccc
      241 cccggcagga cgctccggtc cagcatgagg aggctccgct cccgctcgct ctcgccggcg
      301 atccctatcg cggccttggt ggccgtgccc gaggagagga cgaccccgaa gcggacccgc
      361 ggggccacct gggccacgag gcccttgagc tcgggcctgg cctcgatggc cgcgaccacg
      421 gcgtcggcgt cgccgatggc cgcgttggtc ggcatgaagc gctcccgcct gcggtagtcc
      481 tcggtggcga tgttgacgtg gcccgtctgg ttcttggtgg cgttccgcac gatggagtcg
      541 aggacccccg agatgaagcc gtcggcgaag atgacgatca tcatcgacat gaagaccgaa
      601 aggattgcca ggaagctccg cctcctgttg cgtccgaggt tgcggaacgc gatgcccagt
      661 gtgttcacgg cgatctcctt cacacgaagc gcagggcgtc ggtcggctcg agtttggccg
      721 ccctgcgggc cggtatcctc gcggccacca gggagacgac gacgccgaag acgaagccga
      781 aggccatggt cgtcaggttc cactccccgc gcagcacgcc cgagaggggc aggctgccca
      841 tcgcgtcggc gtaggggtcg aggttgatgc cctgcgagat gagaagccag tccaggaggg
      901 cgccgaggcc gacgcccagg gccgagccga cgaggccgag ggccaggccc tcgagggtga
      961 agagccgggc gatgtccttc ggggtcatgc cgtaggcccg caggaccccg atctcgcgca
     1021 ccctcgagta gaccgacatg aggatggtgt tgacgatgcc cactgccgcg atgatgagga
     1081 ccacgaagac gaggacgaag gagtacttgg ccttggccgt cctgaccgac aggtagtccc
     1141 tggcgaggaa gctgatcggg tccaccctgg cgccgggcag ctgggccctc agcgcggcgg
     1201 ccgccttctc cccctcggcg agggtggtgt tgagggtcgg ctccctcggc agggctgcgt
     1261 cgacctcggt cgcgaagccg cccctcgtct cgggcgccgc gaggagctcg cgcgccgccg
     1321 ccatcggcat gaagagccct gagcggttga cctcgggtgc cggggtgtcc acgaggccga
     1381 cgaccgagta ctcgtcggcg ttcgtggtgt cgttgacagt ctgggccgag acgaggacgg
     1441 agtccccgac cttgatcttg agctcgtcgg cgagggccgc cccgaggacc accgacttgg
     1501 aggccgcccc gtcgagccat gaccccgccg agatggagcg cgccagggca aagaccgacg
     1561 cgtctgtccg ggggtcgacg gcgtctgcca agaggggaat ctcgtcagtg tagttcgaga
     1621 gcttggctat gaagcggatc ctggccgtgg cgctgaggcc ctcggccctg atggcgcgca
     1681 gggctgcctc ggggtcggag aggggcttgt cgagggggct cgcctggatg tcctccacgt
     1741 aggccggatt gcggaccttg agcgaggaga cggtgtactg ggcgaggttg tcgaccgcca
     1801 tcctgtccat gcccgagagc atcgagtcga aggctatgaa gaccgagatc cccgcggtca
     1861 tgacgatcgc ggtgatgatg gtccttcgcc tgtggcgcac cgtgttgcgg agcgcgatct
     1921 tccagagggt gcccatgtca tttcctctcg tcgccggtga ccctgccgtc gcggatcgtc
     1981 acgatgcggc gggcgtgctc catgaccagg cggtcgtggg tggagaacac gaaggtggtc
     2041 tgcctctcct ggttgatcct cctcatgagc tcgacgatct cctcggcggt gtgcgagtcg
     2101 aggttggccg tgggctcgtc ggcgaggacc acgaggggcc gtttcacgag ggcccgggct
     2161 atcgccacgc gctgctcctg gccgcccgag agctcgcggg ggaagcgctc ctccttgccc
     2221 gcgaggccca cggcggcgat catctccctg gccctctcgc cgatctcctt gggcgtgagc
     2281 cgcttctcga tgacgaggga gagctccacg ttctccctca ccgtgaggac ggggatgagg
     2341 ttgtaggact ggaagacgaa gctgatccgc tcgcggcggt aggccgacag gcccttctcc
     2401 ccgagggcgt ggagctcggt gccgtcgtag acgatcgtcc cctcggtggg cgagtcgagg
     2461 cagccaagga tgttgaggaa ggtggtcttg cccgagcccg agggcccgac gagggtgagg
     2521 aactccccgg cctcgatgtc gaggtcgacc ccgtcgaggg ccctgacctc gctctccccc
     2581 ttcctgtata ccttggtcac cttctctaga tgaagcgcag ccatttcaat cctcctctcc
     2641 ggcgcgcgcc tgcgcgccgg cgtcgccctc ggtctgccgt ggccccttga gcgaggcgac
     2701 tatcacctcg agctcggcca gataggccga gagggcgttc tgcccatcca cggtcaggct
     2761 cacgcccgta tagggcttgt tcttcacgaa gcgtttttcg atcttcacgt agcccgcctc
     2821 ctcgagggtc ttcagctgga ccgagaggtt ccccgagctg aagccgagct cgtccttgag
     2881 ctcggtgaac ccagcctcag ccttctcgga ggaggccaga taggtcagga ccatgagcct
     2941 cgccctctcg tggatcaccc tgtcgaggtc gagcttcctg atgccggctc ggggcccccc
     3001 cggttcggag gccgg
//
LOCUS       DASSQV010000395         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_467466, whole genome shotgun sequence.
ACCESSION   DASSQV010000395 DASSQV010000000
VERSION     DASSQV010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467466"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VFL04_06895"
     CDS             complement(<1..860)
                     /locus_tag="VFL04_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET7839471.1"
                     /translation="MGVSTMRIAGTGCCLLDVLYPNIDFSSPAFASCRSRRDGDGGLS
                     PGRLVFDTDLERFTGRPREDALLGIIGGAGPGAENVGGPSIVALIHAAQMLEGRDARV
                     SYYGPRAGDEAGMRLASILARTPVSLEAYSPVDGPCPATIVLSDPHWDSGRGERSFVN
                     ILGAAARYGSDALGGDFFDSDIVAFGGTALTPLLHEALPDLLTRARSRGALTVINTVF
                     DFQAERRDPMGAWPLGGASPGAQPEASAGPGPRESYEGCDLLVMDLEECRRLSGNRDL
                     KTSMDLLRGSG"
     gene            969..>2386
                     /locus_tag="VFL04_06900"
     CDS             969..>2386
                     /locus_tag="VFL04_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET7839472.1"
                     /translation="MIDYRATGLPIVPFLPEVASLLAEGGALCLAAEPGAGKTSLVPP
                     WLAESGRVPGRIIVLEPRRIAAVGAAARMAELMGEGLGAGVGYRVRQDSRVGPSTRII
                     AMTPGVLVRIMQDDPGLAGIGAIVFDEFHERSVQADLCLALALEARALRADLLLLLMS
                     ATLDAERAAEFLGARILAVPGRSHPIAVRHAPLAEGRGFEEGFAALALGLAAEAQGDV
                     LAFLPGGAEIERARRALEAAGMPAFALHGSLPLEAQRAVIAPRPSSPRRVILATSIAE
                     TSLTVPRVAAVLDSGLARLSRFHVRTGLNRLVTERESADRAEQRKGRAGRLGPGLCLR
                     AWAESEILSPRTEPEILRSELSALVLESALWGARGRADLPWLDPPPEPAWEAGRELLA
                     ELGAIEAGGRITAFGKRMAGLGTEPRLAALVLRGQAAGLAEEACLAAALLSEGETGGE
                     RDLAARIGGLMARGARATGGDAR"
BASE COUNT          317 a          875 c          862 g          332 t
ORIGIN      
        1 ccggagcctc tgaggagatc catgctcgtc ttcagatcgc ggttgcctga tagcctgcgg
       61 cattcctcaa ggtccatgac gaggagatcg cagccctcgt aggactccct ggggccgggc
      121 cccgccgagg cttcgggctg ggccccgggc gatgctccgc cgaggggcca tgcccccatg
      181 gggtcccgcc tctcggcctg gaagtcgaat accgtgttga tgacagtgag ggctccgcgc
      241 gagcgcgccc gcgtcaggag gtcaggcagg gcctcgtgga ggaggggggt gagggcggtg
      301 ccgccgaagg ccacgatgtc ggaatcgaag aaatcgccgc cgagcgcgtc gcttccatac
      361 cttgccgccg ctcccaggat gttcacgaag ctccgctcgc cccggcccga gtcccagtgc
      421 gggtccgaga ggacgatggt cgccgggcag gggccgtcga cggggctgta ggcctcgagg
      481 gagacggggg tcctcgcgag gatggaggca agccgcatcc ccgcctcgtc gccagcgcgg
      541 gggccgtagt aggacaccct ggcgtcccga ccctccagca tctgggccgc gtggatcagg
      601 gcgacgatcg agggcccgcc gacgttctcg gcgccggggc ctgccccccc gatgatgccg
      661 agcaacgcgt cctcacgggg ccttccggtg aagcgctcga ggtcggtgtc gaacacgagg
      721 cgccccgggg agaggccgcc gtcgccgtcc ctgcgcgagc ggcaggaggc gaaggccggc
      781 gaggagaagt ctatgttcgg atacaggacg tcgaggaggc agcagccggt tcccgcgatg
      841 cgcatcgtgc tcacgcccac actgtagcgc cttgggcggg cccgagagaa gcccggcgca
      901 ggcgcggggc caggcaggcc ctgacccctc ctcgaccttg gcgggccagc cgttgcacac
      961 tcctgccgat gatcgactac agagccaccg gtctgcccat cgtccccttc ctccccgagg
     1021 tggcgagcct actcgccgag ggcggggccc tctgtctcgc cgccgagccc ggcgcgggca
     1081 agacgagcct cgtgccgccc tggctcgccg aatccggcag ggttccaggc aggatcatcg
     1141 tccttgagcc gaggcggata gcggccgtcg gggctgctgc ccgaatggcc gagctcatgg
     1201 gcgaggggct cggcgccggc gtcggctacc gggtgcgcca ggactcgagg gtcggaccat
     1261 cgacccggat catcgccatg acccccggcg tccttgtgcg gatcatgcag gacgacccgg
     1321 gcctcgcggg gatcggggcc atcgtcttcg acgagttcca cgagcgctca gtccaggccg
     1381 acctctgcct cgccctcgcc ctcgaggcga gggccctcag agccgatctc ctcctcctcc
     1441 tcatgtcggc caccctcgac gccgagcggg cagcggagtt cctgggcgcc cggatcctcg
     1501 ccgtccccgg ccgcagccac cccatcgcgg tgaggcacgc cccccttgcc gagggacgcg
     1561 gcttcgagga aggcttcgcc gccctcgcgc ttggcctggc cgccgaggcc cagggcgacg
     1621 tcctggcctt cctccctgga ggggccgaga tcgagcgggc gaggagggcc ctcgaggcgg
     1681 cgggcatgcc cgccttcgcc ctccacggtt cccttcctct cgaggcccag agggcagtca
     1741 tcgccccccg gccctcctcg ccccgccgcg tcatcctcgc taccagcatc gccgagacga
     1801 gcctcaccgt gccgagggtc gccgctgtcc tcgattcggg cctggccagg ctctcgcgtt
     1861 tccatgtccg caccggtctc aaccgtctgg tcaccgagcg cgagtcggcc gacagggccg
     1921 agcagcgcaa gggccgcgcc ggacgtctgg gcccggggct ctgcctcagg gcctgggccg
     1981 aatctgaaat cctgagtccc cgcaccgagc ccgagatcct gcgctccgag ctctcggccc
     2041 tcgtgctcga gtccgcgctc tggggggcga ggggccgcgc cgacctgccc tggctcgatc
     2101 ccccgcccga gccggcctgg gaggccgggc gggagctcct ggccgagctc ggggccatcg
     2161 aggccggggg tcggataacc gcgttcggaa agcggatggc cggcctgggc acggagccgc
     2221 gtctggcggc cctcgtcctc cgaggccaag cggcaggcct cgccgaggag gcctgcctcg
     2281 ccgcggccct cctctctgag ggggaaaccg gcggggagcg cgacctggcc gccaggattg
     2341 ggggcctcat ggcgaggggc gcgagggcca cgggcggaga cgcccg
//
LOCUS       DASSQV010000396         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1585943, whole genome shotgun sequence.
ACCESSION   DASSQV010000396 DASSQV010000000
VERSION     DASSQV010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585943"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFL04_06905"
     CDS             <1..571
                     /locus_tag="VFL04_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HET7839473.1"
                     /translation="ADEKKLEREAIENKRMAEDWEKKAMLAVRSNRDDLAKEALLRKQ
                     EYEGYSQQLTTQWQAQKESVDKLKESLRQLQNKIDEASRKKNILIARAKRAEAQDKIN
                     KTMSSLSGNRSAFDTFDRMSKKVDELEARTEAVKELDDATSGATLEKQFAALEQTGGA
                     DSLLEDLKRKMLTQDAGSQGAAGGETPKP"
     gene            594..1208
                     /locus_tag="VFL04_06910"
     CDS             594..1208
                     /locus_tag="VFL04_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7839474.1"
                     /translation="MKVRLLACAEPYISYATARDPEGSLVFCEEGGAADRDPPEVLVI
                     PAESFLALGAEARRRQASIAYGPAGLMGACFEAGCVDYLRDPWPLEELAARLGRIERP
                     RFRLGTALVELRGSLLSTDAAEVSLSESERRLLRVLYLNRGRPVPREALRSALGGCFG
                     ASSRLPDVHIASIRKKLEALVPGASSCLRACRGLGYRLDVDACG"
     gene            1520..>2024
                     /locus_tag="VFL04_06915"
     CDS             1520..>2024
                     /locus_tag="VFL04_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839475.1"
                     /translation="MKLWLDTVGRALIEFILIIALVALMGGLDSSVGPGQRDGRSVLL
                     GSARSAIAYMPLAVAATIFLSMFAFERRVSSRAAGWLGLLTIGCLLFAAYFGLLGLSA
                     GGSLARAAGDQAKRTQVQAPQAGLAAERGLSLLWAQDFGPGQAQAVVAADFARPYPRL
                     RYAGTAPY"
BASE COUNT          350 a          685 c          659 g          330 t
ORIGIN      
        1 cgccgacgag aagaagctcg agcgcgaggc catcgagaac aagaggatgg ccgaagactg
       61 ggagaagaag gccatgctgg cggtgcggtc caaccgcgac gacctcgcga aggaagccct
      121 gctcaggaag caggagtacg agggctattc ccagcagctg accacccagt ggcaggccca
      181 gaaagagtcg gtcgacaagc tcaaggagtc cctccgccag ctccagaaca agatcgacga
      241 ggcctcgcgg aagaagaaca tcctcatcgc cagggccaag cgcgccgagg cccaggacaa
      301 gatcaacaag accatgtcga gcctctccgg caacaggagc gccttcgaca ccttcgaccg
      361 catgtccaag aaggtcgacg agctcgaggc caggaccgag gccgtcaagg agctcgacga
      421 cgccacctcg ggggcgaccc tcgagaagca gttcgccgcc ctcgagcaga cgggcggggc
      481 cgacagcctc ctcgaggacc tcaaacgcaa gatgctcacc caggacgcgg ggagccaggg
      541 agccgccggc ggagagactc ccaagccctg agggccccag gcgaggccgg gcggtgaagg
      601 tcagactgct tgcctgcgcc gagccctaca tatcctacgc gacggcgagg gaccccgagg
      661 ggtccctcgt tttttgtgag gagggcgggg ccgcggatcg ggatcccccc gaggtcctgg
      721 tcatcccggc cgaatccttc cttgccctcg gggccgaggc acgacgccgt caggcctcca
      781 tcgcctatgg gcccgccggt ctgatggggg cctgtttcga ggctggctgc gtcgactacc
      841 tcagggaccc ttggcccctg gaggagctcg cggccaggct cgggcggatc gagaggccga
      901 ggttcagact cgggaccgcc ctggtcgagc ttcggggctc cctcctctcc accgacgccg
      961 ccgaggtctc cctgagcgag tccgagcggc gcctgcttcg ggtcctctac ctgaaccgcg
     1021 gaaggccggt gcctcgagaa gcccttcgct cggccctcgg cggctgcttc ggcgcctctt
     1081 cgaggctccc tgacgtccac atcgcgtcaa taaggaagaa gctggaggcc ctcgtccccg
     1141 gggcctcatc ctgcctgcgc gcctgccgcg gcctcggcta ccggctcgac gtcgacgcct
     1201 gtggataagt tgtggggaag tatcgggaaa gaccgggaaa tcgcaggctt ggtatcattt
     1261 tgtatacgac tggaacgatt cggtagacgg atggcggcta acaagtcggc catccgcgtg
     1321 tatgtacttt gctcaactgc aattatctcg atccttggac ctcggagaca ttctggcgcc
     1381 gagcggggtc ccgaggaaac tctttatgcg gtaacccttt ccctcgtacc cgctccggga
     1441 tagcctgtgg ataacttgtg gggcattcgg gaacctggct cggcttgcgg gccccgagag
     1501 cccatgctag attctgggga tgaagctttg gctcgacacc gtcggtcgcg ccttgatcga
     1561 gttcatcctc atcatcgccc tcgtcgccct gatgggcggc ctcgattcct ccgtcggacc
     1621 gggtcagagg gatggaaggt cagtgctcct cggctcggcc cgctcggcca tcgcctacat
     1681 gccgctcgcc gtggcggcga ccatcttcct ttcgatgttc gccttcgaaa gaagggtttc
     1741 gagcagggcg gcgggctggc tcggcctcct caccatcggc tgtctcctct tcgccgccta
     1801 tttcggcctc ctcggcctca gcgcgggcgg ctccctggcc agggccgccg gcgaccaggc
     1861 caaacggacc caagtccagg ccccccaagc cggcctggcc gccgagcggg gtctgtccct
     1921 tctctgggcc caggacttcg ggcccggcca ggcccaagcc gtggtcgccg cagacttcgc
     1981 gaggccctac ccgaggctaa ggtacgcggg gacggccccc tacg
//
LOCUS       DASSQV010000397         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_93383, whole genome shotgun sequence.
ACCESSION   DASSQV010000397 DASSQV010000000
VERSION     DASSQV010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93383"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1090
                     /locus_tag="VFL04_06920"
     CDS             263..1090
                     /locus_tag="VFL04_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7839476.1"
                     /translation="MTSTTIAELEKRAREIRVLTIDAIGFLGVGHIGGAMSIVEALTI
                     LYYRHMDVDPAKPRRPDRDQLVLSKGHAGPALYSTLATKGFFPREWLHSLNVGGTNLP
                     SHCDRNRTPGIDMTTGSLGQGLSSACGIALGNRLNGVCSKVYCIIGDGESDEGQNWEA
                     AMFAAHYRLDNLIAFTDCNKLQIDGATNEVMGLGSLRSKWEAFGWRAEEVDGHDFAAM
                     DGAIERAKAATGQPSMIILHTVKAKGFAPSEGQAASHNQTFSHEEALKAIAALEGCK"
     gene            1090..2028
                     /locus_tag="VFL04_06925"
     CDS             1090..2028
                     /locus_tag="VFL04_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET7839477.1"
                     /translation="MATEKKEMRTVYVETLIDLARANEDIVVLEADLMKATGTGAFAK
                     AFPARAINVGVAEANMVGVASGLSSVGKIPFAATFGSFAGRRDYDQFFLSANYARLNV
                     KLVGTDPGVMAKYNGGTHMPFEDLALMRAIPKLVIVEPSDPTSLERILRLAAAYYGCV
                     YIRLQRTPANVLYGPDETFEFGKAKLLREGKDVLLVALGSIMVNAALAAADELAASGI
                     RATVLDAISLKPLDFAAILEHATRTGRVVTCENAQASGGLGSAVAEALLEAGVSAKFA
                     RIGVKDEFGEVGTVEYLVERYGLGSSHIVAAAKGLM"
BASE COUNT          399 a          847 c          763 g          320 t
ORIGIN      
        1 caaggtggcc atccttctcg acgggacggt gactgtcccc ctgatcgagg cctagggcca
       61 aggggaggcc agctgccgcg gaggccacgg gacggcccga ggcccggcaa tgaccgggct
      121 cgggtccggc ctgggccctg cttgatcttt attttcaaat agaaaataat accgaatgac
      181 cggccgcccc tgccccgggg ccccgccagg gcggggctcg ggcaggcggc ggggacggga
      241 aggcaggaga acacagatgc caatgaccag caccacgatc gccgagctcg agaagcgggc
      301 cagggagatc cgcgtcctca cgatcgacgc catcggcttc ctcggcgtcg ggcacatcgg
      361 cggggccatg tcgatcgtcg aggccctcac catcctctac taccgccaca tggacgtcga
      421 ccccgcgaag ccgcgcaggc ccgaccggga ccagctcgtc ctctccaagg gccacgccgg
      481 ccccgccctc tactcgaccc tagccaccaa gggcttcttc ccccgcgagt ggcttcacag
      541 cctcaacgtc ggcggcacca atctgccgag ccactgcgac aggaaccgga cccccgggat
      601 cgacatgacc acgggctccc tgggccaggg cctgtcctcg gcctgcggga tcgccctggg
      661 caacaggctc aacggggtct gctccaaggt ctactgcatc atcggcgacg gcgagagcga
      721 cgagggccag aactgggagg ccgcgatgtt cgccgcccac taccggctgg acaacctcat
      781 cgccttcacc gactgcaaca agctccagat agacggggcc accaacgagg tcatgggcct
      841 cggctcgctc aggtccaagt gggaggcctt cggctggcgg gccgaggagg tggacggcca
      901 cgacttcgcc gcgatggacg gggccatcga gagggccaag gccgcgacgg gccagccctc
      961 catgatcatc ctccacaccg tgaaggccaa gggcttcgcc ccgagcgagg gccaggccgc
     1021 gagccacaac cagaccttct cccacgagga agccctgaag gcgatcgccg ccctggaagg
     1081 atgcaagtga tggccaccga gaagaaagag atgcgcacgg tctacgtcga gaccctcatc
     1141 gacctcgcca gggcgaacga ggacatcgtc gtcctcgagg ccgacctcat gaaggccacg
     1201 ggcacggggg ccttcgccaa ggccttcccc gcgcgggcga tcaacgtggg cgtggccgag
     1261 gccaacatgg tgggcgtggc ctcgggcctc tcttcggtcg gcaagatccc cttcgccgcc
     1321 accttcggca gcttcgcggg gcgccgcgac tacgaccagt tcttcctctc ggccaactac
     1381 gcccggctga acgtcaagct cgtgggcacc gaccccgggg tcatggcgaa gtacaacggc
     1441 gggacccaca tgcccttcga ggacctcgcc ctcatgcgcg cgatccccaa actcgtcatc
     1501 gtcgagccct cggacccgac gagcctcgag cgcatcctcc gcctcgccgc ggcctactac
     1561 ggctgcgtct acatccgcct gcagagaacg ccggccaacg tgctctacgg ccccgacgag
     1621 accttcgagt tcggcaaggc caagctcctc cgcgagggca aggacgtcct cctcgtggcc
     1681 ctgggctcca tcatggtcaa cgccgccctc gcagccgccg atgagctcgc cgcctcgggc
     1741 ataagggcca ccgtgctcga cgccatcagc ctcaagcccc tcgacttcgc cgccatcctc
     1801 gagcacgcga cgaggacggg ccgcgtggtt acctgcgaga acgcccaggc ctcgggcggc
     1861 ctcggcagcg ccgtggccga ggccctcctc gaggcgggag tcagcgcgaa gttcgcgcgc
     1921 atcggggtca aggacgagtt cggcgaggtg ggcaccgtgg agtacctcgt ggagcgctac
     1981 gggctcggct cttcgcacat cgtggccgcg gcgaaggggc tgatgtaggg ggataagggg
     2041 ggagcgcggc cagtcctgag cgcccgcgct ctccagcgac aggcagatgg cgccgagggc
     2101 cctgcgcatg gggccccgcc ttcgacactg cgtccccctc cggtcggcgc tcatcgaatc
     2161 gcgccgacct cggcctgcgc ttcgtcagaa cagtggcgca ggcctgcgga tggtcggggg
     2221 ccgcagtgtc ggtcgcccca ttcgccacgc ccaatgcgcc atcagcggcc ttgccgagta
     2281 gcgcgggcgt gaggcgcgcg agggcagcca ccatctaccc gctgctccc
//
LOCUS       DASSQV010000398         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1586404, whole genome shotgun sequence.
ACCESSION   DASSQV010000398 DASSQV010000000
VERSION     DASSQV010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586404"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="VFL04_06930"
     CDS             <1..1256
                     /locus_tag="VFL04_06930"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HET7839478.1"
                     /translation="RVLRRDYDKETKLPAALVEEIARETSLSQATWAEARGKSDFAAF
                     APHLEKVVALMKEKAACLGPDRKPYDVLLDLYEPGSTEASVAEVFGSLRKDLVSILGR
                     IRSRPQVDDRALHAHCPKAAQIQISEWLMDVLAYDRGRGRLDQTAHPFTTTLGVDDIR
                     ITTRYLEDYLPSSVFSTMHETGHALYELGIDPAPGFRGTRLAEASSMAIHESQSRLWE
                     NIVGRSQAFWKPNYAKLRSLSGGSLGGLELEGFVRAVNKVEPSFIRTEADEVTYGLHV
                     ILRFELEAELISGRLAARDLPAAWNAKMKELLGIVPPDDARGCLQDVHWSAGLFGYFP
                     SYSLGNLYAAQFWRTMRDTMPGLEAEIEAGRLGGLLSWLRGNIHRPGSTWLPGELVQR
                     VTGGPLDSRHFVEYLGEKYARIYGF"
     gene            1246..2145
                     /locus_tag="VFL04_06935"
     CDS             1246..2145
                     /locus_tag="VFL04_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839479.1"
                     /translation="MASDGRGARLLAAATNPWLWSGLALFLFGLALGQILRALVLRRR
                     GGPASPKAAGIRASLLASLGLLALAALLILPAPEALRSSGLAAWAGAAFALGGFAGFL
                     PMPVGLPLLILAAGSFGLMRSGLAAWLPWDGPGEIARLLPIEVRVEPASRVRAELDLG
                     SGRAEREARMMDLGFASAGLCVEGLELRGPLAALAVLARCDRPPADSGRAARFFRVAG
                     LIGPGEARALLPVSAGPLTRLVGLDALGGFEPGGKAESRALGSLILGRRATSGLQSLE
                     ALSPVSFSIDASLVPSASSADQG"
BASE COUNT          291 a          876 c          743 g          371 t
ORIGIN      
        1 tcagggtcct ccgcagggac tacgacaagg agaccaaact gccggccgcc ctggtggagg
       61 agatcgcgcg cgagacgagc ctctcccagg cgacctgggc cgaggcgcgc gggaagagcg
      121 acttcgccgc cttcgcgccc cacctcgaga aggtggtggc cctcatgaag gagaaggccg
      181 cctgtctcgg gccggacaga aagccctacg acgtcctcct tgacctctac gagccgggca
      241 gcaccgaggc ctcagtggcc gaggtcttcg gctcgctccg caaggacctc gtctccatcc
      301 tgggcaggat ccgatcccgg ccccaggtcg acgacagggc cctccacgcc cactgcccca
      361 aggccgccca gatccagatc agcgagtggc tcatggacgt cctggcctac gaccgcggcc
      421 gcggccgtct ggaccagacc gcccatccct tcaccacgac cctcggcgtg gacgacatca
      481 ggatcacgac gcgctatctc gaggactacc tgccctcgag cgtcttctcc acgatgcacg
      541 agaccggaca cgcgttgtac gagctcggga tcgaccccgc gcccggcttc cgcgggacca
      601 ggctcgccga ggcgagctcg atggccatcc acgagtccca gtcgcggctg tgggagaaca
      661 tcgtgggccg gtcccaggcc ttctggaagc ccaactacgc gaagctccgc tccctctccg
      721 gcgggagcct cggcggcctc gagctcgagg gcttcgtgag ggcggtcaac aaggtcgagc
      781 cctccttcat ccgcaccgag gccgacgagg tgacctatgg cctccacgtc atcctgcgct
      841 tcgagctcga ggccgagctc atctcgggcc gcctggccgc ccgcgacctg ccggcagcct
      901 ggaacgccaa gatgaaggag ctgctcggga tcgtcccgcc ggacgacgcc aggggctgtc
      961 tccaggacgt gcactggtcg gccgggctct tcggctactt ccctagctac tcgctcggca
     1021 acctctacgc ggcccagttc tggaggacga tgcgcgacac catgcccggg ctcgaggccg
     1081 agatcgaggc cgggaggctc ggcggcctcc tctcctggct gcgcggcaac atccacaggc
     1141 cgggctcgac ctggctaccc ggcgagctcg tccagcgcgt gaccggcggc cccctcgact
     1201 cgaggcactt cgtcgagtac ctgggggaga agtacgcccg catctatggc ttctgatggt
     1261 cgcggcgcga ggctcctcgc cgccgcaacc aacccctggc tctggtctgg cctggccctc
     1321 ttcctcttcg gcctcgccct cggccagatc ctgcgcgccc ttgtcctgcg gcggcgggga
     1381 ggtccggcct cgcccaaggc cgcgggcatc agggcctcgc tcctcgcgag cctgggcctc
     1441 ctcgccctcg ccgccctcct gatcctgccg gcccccgagg ccctgcgctc gtccggcctt
     1501 gcggcctggg cgggggcggc cttcgccctc gggggctttg cgggctttct gccgatgcct
     1561 gtcggccttc ccctcctcat cctcgcggcg ggctcattcg ggctgatgcg ctcggggctc
     1621 gcggcctggc tcccctggga cggacccggc gagatcgccc gtctcctccc catcgaggtc
     1681 cgggtcgagc cggcctcccg tgtccgcgcc gagctcgacc ttggctcggg ccgcgccgag
     1741 agggaggcgc gcatgatgga cctcggtttc gcctcggctg gtctctgcgt cgagggcctc
     1801 gagctgcgcg gccccctcgc cgccctggcc gtcctcgccc gctgcgaccg tccgcccgcc
     1861 gactcgggga gggccgcgcg tttcttccgc gtcgccggcc tcatcgggcc gggcgaggcc
     1921 agggccctcc tccccgtgtc ggccggtccc ctcacccgcc tcgtgggcct cgatgccctt
     1981 gggggcttcg agcccggggg caaggccgag agccgggccc tcggttcgct catcctgggc
     2041 cgccgcgcca cgagcgggct gcagagcctg gaggccctga gccccgtcag cttctcgatc
     2101 gacgcctccc tcgtcccctc ggcctcgtcc gcggatcagg gctgaaagcc ccagccgggc
     2161 ctgcttcata cccctggggt atcggcgcgg gcgaattggg aattggacga ggcctttcgt
     2221 tttcgagtaa gctgtggcac acatgccgca gctctccatc caattcgccg agatttttct
     2281 g
//
LOCUS       DASSQV010000399         3237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_219039, whole genome shotgun sequence.
ACCESSION   DASSQV010000399 DASSQV010000000
VERSION     DASSQV010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219039"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..901)
                     /locus_tag="VFL04_06940"
     CDS             complement(155..901)
                     /locus_tag="VFL04_06940"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7839480.1"
                     /translation="MAELPPVIRSRNLADQVTDILRERILARVYPPDQRMPSEAQLAE
                     EFHVSRSSLRTALASLAAEGHIRRRHGDGTYPCPPNFRVTLRSGSDWDIERQIEHSGR
                     AAAMRILSQGFVTPDAEEARRLGLAGEALVYSIRRLFLADDLPVALIDTSLLAEGLSA
                     DIPASEAALPPQRFLELYQEHRARDGEMRFRAIAADAELAALLEVIPGAPLLSIDAVY
                     FDASRQPLMVAHELYRGDQGFEMETGLFGA"
     gene            complement(1197..2915)
                     /gene="ade"
                     /locus_tag="VFL04_06945"
     CDS             complement(1197..2915)
                     /gene="ade"
                     /locus_tag="VFL04_06945"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HET7839481.1"
                     /translation="MIEKDLIDAAQGAAPCDLNLANCVLVDLYRGEMRRGSTVSIRKG
                     RIAGVNDGLRAERTVDLKGLYLSPGLIDAHVHIESSLLSPPEYARVVAPHGTTAVVAD
                     PHEIANVMGYEGMRYMLASSEGLPIDVFLMVPSCVPATEFDTAGAALYASDMHPFLRE
                     ARVLGLGEVMNYPGVLYKDQKLLDKIALFRDAGKPVDGHAPGLTGGALSAYIAAGIGS
                     DHECTSPEEAAEKLAKGMYVMLREGSTAKDLRTLLPAVNPRTASRVLICSDDRHANDL
                     VDEGHMDHALRLLLAGGVSPIDAFTIACLNPARWFNIPGAGAIAPGFKADLVAFESLE
                     DFRAKLVFKDGVLVAEDGRLVADTPTARPPTRDSVNIKWLTEEDFRIPAQGRRIRAIE
                     ASPGSIITGERVLEARIEEGLCVSDLGRDLVKIFVIERHTGSGNIGKGFITGLGLKRG
                     AVGATVSHDSHNMIVAGVDDASIFKAARHLNKMKGGFVFAVGDEVILDLPLPVAGLMS
                     DRDASFVVDRLRAFEALFEREGLALASPLMTLSFMALPVIPSLKITDRGLIDVVRFSP
                     VGLFVD"
     gene            complement(2965..>3237)
                     /locus_tag="VFL04_06950"
     CDS             complement(2965..>3237)
                     /locus_tag="VFL04_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7839482.1"
                     /translation="GGRGWIVIALVIFSTWNPARAIWGALLFGGINAVQFRLQAAGTT
                     IPAGILNMLPYLATIVVLVLITWWEALSKRVGAPASLGNAYMREEK"
BASE COUNT          519 a         1031 c         1145 g          542 t
ORIGIN      
        1 agcagtcgtc ctcacacagt ccttccctgc cgccgcttgg agcgggatcc tgtcgagatg
       61 ccatgcccct atgaatggtg ataagacagt cgtcatacca ctttaggggc agtatccgcc
      121 tacgtcaatc gaaattggga ccatcctccc cttttcaggc tccgaagagg cccgtctcca
      181 tctcgaaacc ctggtccccc cggtacagct catgggcgac catgaggggc tgccgcgatg
      241 cgtcgaaata gacggcgtct atggaaagga ggggagcacc cgggatcact tcgaggaggg
      301 cggccagctc ggcgtcggcc gcgatggcgc ggaagcgcat ctcgccgtcc ctggccctgt
      361 gctcctggta gagctcgagg aaacgctggg gggggagggc ggcttccgag gccgggatgt
      421 cggcggaaag gccctcggcg aggagggagg tgtcgatgag ggccacgggc aggtcgtcgg
      481 cgaggaaaag gcgtcggatc gaatagacca gagcctcccc ggcgaggccg agcctccggg
      541 cctcctctgc gtccggcgtc acgaagccct ggctcaggat cctcatggca gccgccctgc
      601 ccgaatgttc gatctggcgc tcgatgtccc agtccgagcc ggagcggagg gtcacgcgga
      661 agttgggggg gcagggatag gtgccgtcgc cgtggcgcct ccggatgtgg ccctcggcgg
      721 ccaggctcgc cagggccgtc ctgagcgagc tcctgcttac gtggaactcc tcggccagct
      781 gggcctcgga gggcatgcgc tggtcgggag gatagaccct ggccaggatg cgctccctga
      841 ggatgtcggt cacctgatcg gcgaggttcc gggatcgtat tacgggcggc agctcagcca
      901 tgggcggttt tctgttcccc ctaactcatg aagacaaccg cagaggcgca gaggcgcaga
      961 ggcgcggagt tgacacagag aagaaagacg caggcgaaaa agcgaaggaa gctatccaca
     1021 ggaggctctc tcttgcatcg accccttcca cgccatcctg tctcttctcc ctctttgctc
     1081 tgtgtctctt cgtctctgtg gttcctattc tcccaaactc tctcgcctcg cggcgataat
     1141 cggcagtcgt catacctcta taccactttc ggccttttcc tgccagtggg gccctcctag
     1201 tcgacgaaaa gccccaccgg gctgaaacgc accacgtcga tgaggcccct gtcggtgatc
     1261 ttgaggctcg ggatcacggg cagggccatg aaggacaggg tcatgagggg ggaggcgagg
     1321 gccaggccct ccctttcgaa gagggcctcg aaggccctga gcctgtccac cacaaaggag
     1381 gcgtcgcggt ccgacatgag gccggcgacc ggaaggggga ggtcgaggat gacctcgtcg
     1441 cccacggcga agacgaagcc ccccttcatc ttgttgagat ggcgggcggc cttgaagatc
     1501 gaggcgtcgt cgacccccgc gacgatcatg ttgtgcgagt catggctcac ggtggccccg
     1561 accgccccgc gtttgaggcc caggccggtt atgaagccct tgccgatgtt gcccgagccg
     1621 gtgtggcgct cgatgacgaa gatcttcacg aggtcgcggc cgaggtcgct gacgcagagc
     1681 ccctcctcga tcctggcctc gaggacccgc tccccggtga tgatcgagcc gggcgaggcc
     1741 tcgatggccc tgatcctgcg gccctgggcg gggatgcgga agtcctcctc ggtgagccac
     1801 ttgatgttca ccgagtcgcg ggtgggcggc ctggccgtgg gcgtgtcggc cacgaggcgg
     1861 ccgtcctcgg ccacgaggac cccgtccttg aagaccagct tcgcgcggaa gtcctcgagg
     1921 ctctcgaagg ccacgaggtc ggccttgaag ccgggggcga tcgccccggc gcccgggatg
     1981 ttgaaccagc gcgccgggtt gaggcaggcg atggtgaagg cgtcgatcgg ggagaccccg
     2041 ccggcgagga ggagccgcag ggcgtggtcc atgtggccct cgtcgacgag gtcgttggcg
     2101 tggcggtcgt ccgagcagat gaggacgcgc gaggccgtcc tcgggttcac cgcgggcagg
     2161 agggtccgca ggtccttggc cgtcgagccc tcgcgcagca tgacatacat gcccttggcg
     2221 agcttctcgg cagcctcctc ggggctcgtg cactcgtggt cggagccgat ccccgcggcg
     2281 atgtaggccg agagggcccc gcccgtaagc cccggggcgt ggccgtccac aggcttaccc
     2341 gcgtcgcgga agagggcgat cttgtccagg agcttctggt ccttgtagag gacccccggg
     2401 tagttcatca cctctccgag gcccaggacc ctcgcctcgc ggaggaaggg atgcatgtcc
     2461 gaggcgtaga gggcggcccc cgccgtgtcg aactcggtcg cggggacgca ggaggggacc
     2521 atgaggaaga cgtcgatggg caggccctcg ctcgaggcca gcatgtagcg catgccctcg
     2581 tagcccatga cgttggcgat ctcgtggggg tcggccacca cggccgtcgt cccgtggggg
     2641 gcgacgaccc gggcgtactc gggcggggag aggagggagg actcgatgtg gacgtgggcg
     2701 tcgatgaggc cgggggacag atagaggccc ttgaggtcga ccgtcctctc ggcccgcagc
     2761 ccgtcgttca ccccggcgat cctgcccttc ctgatcgaga ccgtcgagcc ccgtcgcatc
     2821 tcgccgcggt agaggtcaac gaggacgcag ttggcgaggt tgaggtcgca gggcgcggcg
     2881 ccctgggccg cgtcgatcag gtccttctcg atcatcgtct gtctccccgc tgacgccgcc
     2941 ggatcgctgc cgcggcgtcg gctctcattt ttcctcgcgc atgtaggcgt tgccgaggga
     3001 ggccggggcc cccacccgct tggacagggc ctcccaccag gtgatgagga cgaggaccac
     3061 gatcgtggcc aggtagggca gcatgttgag gatgcccgcc ggtatcgtgg tgcccgccgc
     3121 ctggaggcgg aactggacgg cgttgatccc gccgaagagc agggcccccc agatggccct
     3181 ggccgggttc caggtggaga agatcaccag ggctatgacg atccagcccc ggcctcc
//
LOCUS       DASSQV010000400         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1586472, whole genome shotgun sequence.
ACCESSION   DASSQV010000400 DASSQV010000000
VERSION     DASSQV010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586472"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..922)
                     /locus_tag="VFL04_06955"
     CDS             complement(206..922)
                     /locus_tag="VFL04_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7839483.1"
                     /translation="MEEGEHRPRVLVVEDDREIAELVAAYLGRDGALVELVGSAEAGL
                     ESAAVTEPELVLLDLGLPGADGLEFLRAFRARSTAPVIIVSARESDEDKIAGLGLGAD
                     DFVSKPFSPRVLAARARAQLRRASYPKDAAGHGPGKRLGFGPYSLDLEARLLERGGQR
                     VALSRREFELLAYLALNAGQTFAPAELYRAVWGLEHGDLSTVAVHVQRIRRKIEAEPS
                     EPRWLVTVPGQGYRFVPGPS"
     gene            1112..1897
                     /locus_tag="VFL04_06960"
     CDS             1112..1897
                     /locus_tag="VFL04_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7839484.1"
                     /translation="MVIVELQDVRKVYPLGKVQVEAVKGVSFCIEKGDFISIAGPSGS
                     GKTTILNMIGCVDRPTAGTVSIAGRQTKDLDDRELTTLRHETLGFIFQSFNLIPVLSV
                     YENIEFPLLLGKQRMQPKEKREWIDYLVEEVGLADWRKHKPNELSGGQRQRVAIARAL
                     AGKPGIVLADEPTANLDSATGEQILELMKKINREMQTTFIFSTHDAKIVGLADHVIRL
                     RDGLVTENLRNGKDASLLCAETAAVESGSPTAAVGSGSPTAGA"
     gene            1903..>2737
                     /locus_tag="VFL04_06965"
     CDS             1903..>2737
                     /locus_tag="VFL04_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839485.1"
                     /translation="MPVLVRIAFRNLLEHKAKSLIIGILLALGVVILVVGNSFMDTAA
                     KGVKDTFIGNYTGDLFIAPKVKATVSLFGVASVGGREDTPSLPFHDRIVAHLEADPAV
                     AGVTSQVTGFALVSPKDSADQGFAMLFGIDPATYYRLFQNARVVEGRLLKPGEEGLVI
                     SRSQVDNYKKNFGFTPKVGEDLILTGMNKGGFKIRSVPLVGIIAFNTESEATDFISYA
                     DVNTVRILSGLTLGGDEDNPVSPDQKALLAANEDSLFGGESVVAAGKLNAAAAAPAPA
                     RA"
BASE COUNT          477 a          951 c          917 g          392 t
ORIGIN      
        1 tgggcgtcct cgacgagggc gccgcgcagg gccctggcgg tgcgcacgaa acgcgagaag
       61 gggtcgccgg ggtcggaggc gcccacgagg agccgtatga ggatgaagag ggccaggggc
      121 aggatgagcc cgatgagggc caggaagccg aggaggggcc ggagcggcac gcccccgcgg
      181 ccgaatggcg gccgagctcg ccccatcatg acggccccgg gacgaagcgg tagccctggc
      241 cgggcaccgt gacgagccag cggggctccg agggctcggc ctcgatcttg cggcgtatcc
      301 gctggacgtg gaccgccacc gtcgagaggt cgccgtgctc gaggccccac actgccctgt
      361 agagctcggc cggggcgaag gtctggcccg cgttgagcgc aagataggcg aggagctcga
      421 attcgcgccg gctcagggcc accctctgcc caccgcgctc gaggagcctc gcctcgaggt
      481 cgaggctgta ggggccgaag ccgagccttt tcccaggacc gtggccggcc gcgtccttcg
      541 ggtacgaggc ccgtctgagc tgggcccggg cccttgccgc gaggaccctc ggcgagaagg
      601 gcttggacac gaaatcgtcg gccccgaggc cgaggccggc gatcttgtcc tcgtccgact
      661 cgcgggccga gacgatgatg acgggtgccg tgctgcgggc acggaaggcg cggaggaact
      721 cgaggccgtc ggctccggga agcccgaggt cgaggaggac gagctcgggc tcggtgacag
      781 cggcgctctc cagcccagcc tcggccgagc cgacgagctc gaccagggcc ccgtcgcggc
      841 cgaggtaggc ggcgacgagc tcggcgatct ctcggtcgtc ctcaaccacg aggaccctcg
      901 gcctgtgctc cccttcttcc atggtcgaag tatatgtccc cgcccctccc cggctccagc
      961 tccccccgat gaacgatcga tgaattcagg cttccggatg cccgtgttcg gggcccctcg
     1021 ccttgaaaac cgcgtgggcc cgtcgctatc ttggtccctg acggcggggc gccgccctga
     1081 tgaacggggg cgcacgcgaa aaggagtttg tatggtgatc gtagagctcc aggacgtccg
     1141 caaggtctat cccctcggca aggtccaggt ggaggcggtg aagggcgtgt cgttctgcat
     1201 cgagaaggga gacttcatct cgatagcggg accatcgggg tcgggcaaga ccacgatcct
     1261 caacatgatc ggctgcgtgg acaggcccac ggcgggcacc gtgagcatcg cggggaggca
     1321 gaccaaggac ctcgacgaca gggagcttac caccctcagg cacgagaccc tcggcttcat
     1381 cttccagtcc ttcaacctca tacccgtcct ctcggtctac gagaacatcg agttccccct
     1441 cctcctcggc aagcagagga tgcagccgaa ggagaagcgg gagtggatcg actacctcgt
     1501 cgaggaggtg gggctcgccg actggagaaa acacaagccc aacgagctct ccggcggcca
     1561 acgccagagg gtggccatcg cgcgagccct ggccggcaag cccggcatcg tcctcgccga
     1621 tgagcccacg gccaacctgg actcggccac gggggagcag atcctcgaac tcatgaagaa
     1681 gatcaaccgc gagatgcaga ccaccttcat cttctcgacc cacgacgcga agatcgtggg
     1741 cctggccgac cacgtgatcc gcctccgcga cggcctcgtg accgagaacc tccgcaacgg
     1801 caaggacgcc tccctgctct gcgccgagac ggcggccgtc gagtcgggct ctccgacggc
     1861 ggccgtcgga tcgggctctc cgacggcggg ggcctgagcg ccatgcccgt cctcgtgcgc
     1921 atagccttca ggaacctcct ggagcacaag gcaaaaagcc tcatcatcgg gatcctcctg
     1981 gccctcggcg tggtcatcct cgtcgtcggc aacagcttca tggacacggc cgcgaagggg
     2041 gtcaaggaca ccttcatcgg caactacacg ggcgacctct tcatcgcccc caaggtcaag
     2101 gccacggtga gcctcttcgg cgtggcctcg gtgggcggca gggaggacac gccctccctc
     2161 cccttccacg acaggatcgt ggcccatctc gaggccgacc ccgcggtggc cggcgtcacc
     2221 agccaggtga cgggcttcgc cctcgtgtcg ccgaaggact cggccgacca gggcttcgcg
     2281 atgctcttcg ggatagaccc agccacctac tacaggctct tccagaacgc ccgcgtggtc
     2341 gagggtaggc tcctcaagcc cggcgaggag ggcctcgtca tctcgcgcag ccaggtggac
     2401 aactacaaga agaacttcgg cttcaccccc aaggtcggcg aggacctcat cctcaccggc
     2461 atgaacaagg gcggcttcaa gatccgctcc gtgcccctcg tgggcatcat cgccttcaac
     2521 accgagagcg aggccactga cttcatctcc tacgccgacg tgaacaccgt ccgcatcctc
     2581 tcgggcctca ccctcggcgg ggacgaggac aacccggtga gccctgacca gaaggccctt
     2641 ctcgcggcca acgaggactc gctcttcggc ggcgagagcg tcgtcgccgc gggcaagctc
     2701 aacgcggccg cggcggcccc cgcgcctgcc cgggcag
//
LOCUS       DASSQV010000401         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2333021, whole genome shotgun sequence.
ACCESSION   DASSQV010000401 DASSQV010000000
VERSION     DASSQV010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2333021"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFL04_06970"
     CDS             complement(<1..729)
                     /locus_tag="VFL04_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7839486.1"
                     /translation="MRSKSRFALVLLAAILCAFPLGAAFAAPASRGPLFIDLNAQSLY
                     ARVGFDAGALTALPAPGARGWKTVPPAGAGGRTVRPADLGFEGYPQRRPFSLERFRLM
                     EFTFLIPFQVTPAMAALLAPDSPARAAMVPGISFAGLGDNWEVYLNGSLVKSEMHLSD
                     KGGIALHRSMRGVHFPLDPRLLREGQNILALRIVADPSFLPSGFNQASPYYIDDYERI
                     EAANADIWPMILIGLYLFIGLYHIF"
     gene            926..1714
                     /locus_tag="VFL04_06975"
     CDS             926..1714
                     /locus_tag="VFL04_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7839487.1"
                     /translation="MAEITQARTALRMVDIEKYFGSVCALRRVNFEVGRNEIVGLIGD
                     NGAGKSTLVKILTGVFPPTSGELYIADRRVDTRSYSVGKAHELGIETVYQDKSLGEKQ
                     AMWRNFFIGRAITLPFGFLNVKEEKRIAAEIMLKTIGFRSRGIGVDSRANKLSGGERQ
                     GVAIGRAMHFEAELIILDEPTVALSLKEVRKVLDFVHRIKEGGKASIYISHSITDVYE
                     VCDRFIVIDRGEIVASIAKAEISLKALDEYLLEYSHGLRDEEGQ"
     gene            1711..2757
                     /locus_tag="VFL04_06980"
     CDS             1711..2757
                     /locus_tag="VFL04_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7839488.1"
                     /translation="MNRGSQRLAFLGRIEGLPIAAVLAVLYAVFILTAPQVFTGYRIY
                     MSFFQTVPPMLVLALGLTFVITAGEIDLSFPAVVALSGFIFTWVFKNLGAPWLGFALA
                     LASGALVGYLNGLLVARLRVPSIMATLATQFFWYGVTLLIASGLQINIESVTATAIHA
                     IFVGRLFGVFPVQALWAFALTVFLWFILNRHRFGEAVMFIGDNAEVARVMGINVEAMR
                     MRLFVVHGVIAAFAGLLLTLEINVFFPTQGQGMLLPVMAAVFIGGTSIAGGQPVLVGT
                     FFGSYIIGSLEAGVVATGIGGYWVQLVEGIVMAASVILNVVLGEEGAALLSRTARRWA
                     GGEGKERTGGGSRA"
BASE COUNT          460 a          970 c         1038 g          483 t
ORIGIN      
        1 gaagatgtgg tagaggccga tgaagaggta gagcccgatg aggatcatgg gccagatgtc
       61 ggcgttggcc gcctctatcc tctcgtagtc gtcgatgtaa tagggcgagg cctggttgaa
      121 gcccgagggg aggaaggagg ggtcggcgac tatcctgagg gccaggatgt tctggccctc
      181 gcggaggagc ctcgggtcga gggggaagtg gactccgcgc atgctgcggt gcagggcgat
      241 cccgcccttg tccgagaggt gcatctccga cttgacgagg ctgccgttga ggtagacctc
      301 ccagttgtcc ccgaggccgg cgaagctgat gccggggacc atggcggcgc gggccgggga
      361 atcgggggcc aggagggcgg ccatcgcggg cgtgacctgg aaggggatga ggaaggtgaa
      421 ctccatgagc cggaagcgct cgagggagaa gggcctgcgc tgggggtagc cctcgaagcc
      481 gaggtcggcc ggcctcaccg tgcgtcctcc cgcgcccgcg ggggggacgg tcttccagcc
      541 cctggcccct ggggcgggca gggcggtgag ggctccggca tcgaagccga ccctcgcgta
      601 gaggctctgg gcgttgaggt cgatgaagag ggggccgcgg gaggcggggg ccgcgaaggc
      661 ggcaccgagc gggaaggcgc acaggatggc ggcaaggagg acgagggcga agcggctttt
      721 tgatctcatg ggccggaagt ataggacctg cgcggggccc acgcaatacg aggccgcggc
      781 ccggaggggg cggcctcgtg cccggaggcg gcggccgcgg cccggagggg gcggcctcgt
      841 gcccggagcc gccctgggcc cgaccctccc ctgttgcatt gccccggcga cgatgctagg
      901 atttgttcgc ttaaggaggc gacccgtggc tgagatcacc caggcccgca ccgcgctccg
      961 catggtcgac atcgaaaaat acttcggctc ggtctgcgcc ctcaggaggg tcaacttcga
     1021 ggtggggcgc aacgagatcg tcggcctcat cggcgacaac ggggccggca agtccaccct
     1081 ggtcaagatc ctcacagggg tcttcccgcc caccagcggc gagctctaca tcgccgaccg
     1141 cagggtggac acccggagct actcggtcgg gaaggcccac gagctgggca tcgagaccgt
     1201 ctaccaggac aagtccctcg gcgagaagca ggccatgtgg aggaacttct tcatcggccg
     1261 ggccatcacc ctccccttcg gcttcctcaa cgtgaaggag gagaagcgga tcgccgccga
     1321 gatcatgctg aagacgatcg gcttccgcag caggggcatc ggagtcgact ccagggcgaa
     1381 caagctctcg ggcggggagc gccagggcgt ggcgatcggc agggccatgc acttcgaggc
     1441 cgagctcatc atcctcgacg agccgacggt cgcgctctcg ctcaaggagg tgcgcaaggt
     1501 cctggacttc gtgcaccgga tcaaggaggg cggcaaggcg agcatctaca tctctcacag
     1561 catcaccgac gtctacgagg tctgcgaccg cttcatcgtc atcgaccggg gcgagatcgt
     1621 ggcgagcatc gcgaaggcgg agatatcgct caaggccctg gacgaatacc tcctggagta
     1681 ctcgcacggc cttagggacg aggaggggca atgaaccggg gaagccagcg cctcgccttc
     1741 ctgggtcgga tcgagggcct cccgatcgcc gccgtcctcg cggtgctcta cgccgtcttc
     1801 atcctcaccg cgccccaggt cttcaccggc taccgcatct acatgtcctt cttccagacc
     1861 gtgcccccga tgctcgtcct cgccctgggc ctcaccttcg tcatcacggc gggcgagatc
     1921 gacctgagct tccccgccgt ggtggccctc tcgggcttca tcttcacctg ggtcttcaag
     1981 aacctcggcg cgccctggct cggcttcgcc ctggccctcg cctcgggggc cctcgtcggc
     2041 tacctgaacg gcctcctcgt ggcccggctg cgcgtgccct cgatcatggc caccctcgcc
     2101 acccagttct tctggtacgg ggtgaccctc ctcatcgcct cgggcctcca gatcaacatc
     2161 gagagcgtca cggccacggc catccacgcc atcttcgtgg gccggctctt cggggtcttc
     2221 cccgtccagg ccctctgggc cttcgccctc acggtcttcc tctggttcat cctcaaccgc
     2281 caccgcttcg gcgaggccgt catgttcatc ggcgacaacg ccgaggtggc cagggtgatg
     2341 ggcatcaacg tcgaggccat gcggatgcgg ctcttcgtcg tgcacggggt catcgccgcc
     2401 ttcgccggcc tcctcctcac cctcgagatc aacgtgttct tcccgaccca gggccagggc
     2461 atgctcctgc ccgtcatggc ggccgtcttc atcggcggaa cctcgatcgc cggcggccag
     2521 cccgtcctcg tggggacctt cttcggctcc tacatcatcg gctccctcga ggcgggggtc
     2581 gtcgccacgg gcatcggcgg ctactgggtc cagctcgtgg agggcatcgt catggccgcc
     2641 tcggtgatcc tcaacgtcgt cctcggggag gagggcgcgg ccctcctctc gcgcaccgcg
     2701 cgcaggtggg cgggcggcga ggggaaggag aggacaggcg ggggcagccg ggcctgaagc
     2761 ccatcaagcc cgagggcgga cgacccgagc caggggaggc cgtcccgggg aaggaggtgc
     2821 ggcatagtct catgcttttg gcggtccggc gccgtcgagg cgcgcggggc cgcaatccac
     2881 aaaagttggg ggataccatg ggcaggatca agtgcgtcat tctcgcggtg gtggtccttg
     2941 gcctcgtttt c
//
LOCUS       DASSQV010000402         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1586562, whole genome shotgun sequence.
ACCESSION   DASSQV010000402 DASSQV010000000
VERSION     DASSQV010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586562"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFL04_06985"
     CDS             complement(<1..361)
                     /locus_tag="VFL04_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839489.1"
                     /translation="MRRTIASLILAASALPLICQAQPAPPAEGFRQDGSLYYATASGT
                     GPSQGEAQENARGAALRSLFTGLGKDRLYAEVFMGSPPVGLSFTVESATKASSGYVAV
                     VSLRIDDESIRIIERGPY"
     gene            complement(372..1238)
                     /locus_tag="VFL04_06990"
     CDS             complement(372..1238)
                     /locus_tag="VFL04_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839490.1"
                     /translation="MKFRTIFILFNIVIIVSFLFVFLLPFFLLGAEYSLGFWKGNWYL
                     ALFFLALIAALNAFFLFNRKVFTLVESEDWGALSAHLVDLIFVQHKYRPTHIRLLVNA
                     YLLQSNVEGIERLEAELAAKKPALLRSNALLFGVTRLLRNRPAEAEAFFAKYLEAREV
                     QTRDWLRFDYAFSLVLQRRIADAVPILREGALSRDAVLSLLSAYLLGSLGAASLSEQE
                     GRAELVAFAEAARARLTKRFGGPRWSREVDRAKNEVHIVILSKLIDDAGAWLFAGADK
                     TASTTSSGSPGK"
     gene            1369..>2380
                     /locus_tag="VFL04_06995"
     CDS             1369..>2380
                     /locus_tag="VFL04_06995"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET7839491.1"
                     /translation="MNTISLKDLKSKVFFSSPLFLDSGYILLAPETPVDEALIKRLTR
                     WEFREVHSGGQMLDAAAHEAETAEPEAELKPVVAEGSGDRERLEALVNFYLEFTAYVD
                     SLYTRFVTKTELDFPELSNRIKALYGLIVENRRFLLRAQSQAPAHKNYLVSHAVRSAV
                     FSIILGNALKLPPFRLIELGAAAVLHEIGMIRLPSHLYMAGRQLSPVERKSITTHPVL
                     GYNLLKERQVPLAVCLAALEHHERLNGGGYPRGITGDKISLYSRIIMVACSYDAVTAD
                     RPFKPARDGYSGMVDILRNEGKQYDENVIRALVFSLSIFPIGTYVMLSNGKGGLVVDV
                     NPE"
BASE COUNT          433 a          764 c          801 g          382 t
ORIGIN      
        1 ggtaggggcc ccgctcgatg atgcggatcg actcgtcgtc gatcctgagg ctgacgacgg
       61 cgacgtagcc cgaggaggcc ttggtcgcgg actccaccgt gaaggagagg cccacgggag
      121 gggagcccat gaacacctcg gcgtagagcc tgtccttgcc cagaccggtg aacagggagc
      181 gcagggccgc cccgcgggcg ttctcctggg cctcgccctg gctcggcccc gtgcccgagg
      241 cggtcgcgta atagagggag ccgtcctgcc ggaagccctc ggccgggggg gcaggctgag
      301 cctggcagat gaggggcagg gccgaggccg cgaggatgag ggaggctatc gtgcgccgca
      361 tgccgccccc ctcacttccc gggcgagccg ctcgacgtgg tcgaggccgt cttgtcggct
      421 ccggcgaaca gccaggctcc ggcgtcgtcg atgagcttgg agaggatgac gatgtggacc
      481 tcgttcttgg ccctgtccac ctcgcgcgac cagcgcggac ccccaaagcg cttcgtgagc
      541 ctcgccctgg cggcctcggc gaaggcgacc agctcggccc tgccctcctg ctcgctgagg
      601 gaggccgccc caaggctgcc gaggaggtag gcgctgagga gggagaggac ggcgtcccgg
      661 gagagggccc cctcgcggag gatcgggacg gcgtcggcta tgcggcgctg gaggacgagg
      721 ctgaaggcgt agtcgaagcg gagccagtcc ctggtctgga cctccctggc ctcgaggtac
      781 ttggcgaaga aggcctcggc ctcggcgggc cggttgcgca agagtcgggt gacgccgaag
      841 aggagggcgt tggagcgcag gagggccggc ttcttggcgg ccagctcggc ctcgagcctc
      901 tcgatcccct cgacgttgga ctggaggagg taggcgttga cgaggagcct gatgtgggtc
      961 gggcggtact tgtgctgcac gaagatgagg tcgacgaggt gggcggagag ggcgccccag
     1021 tcctcgctct ccaccagggt gaagaccttg cggttgaaga ggaagaaggc gttgagggcg
     1081 gcgatgaggg ccaggaagaa gagggcgaga taccagttgc ccttccagaa gccgagggag
     1141 tattctgctc caagcaggaa aaacggcaag aggaatacaa aaaggaacga aacgatgatg
     1201 acgatgttaa aaagaatgaa gattgtccta aatttcaatt gcagtcgctc ctataattaa
     1261 tgatagggcc gtgcatcgtg aagaaggatc gctgaggcgg atcgtgccgc accgatgcca
     1321 aggtcgaaag tgtagacccg gctctccggc ggggtcaatg ggtggtacat gaacaccatt
     1381 tcgctgaagg acctgaaatc caaggtcttc ttctcatcgc cgctcttcct cgactcaggc
     1441 tacatactcc tggcgcccga gacccctgtg gacgaggccc tcataaagcg gctcacccgc
     1501 tgggagttcc gcgaggtcca ctcgggcggg cagatgctgg acgcggcggc ccacgaggcc
     1561 gagaccgccg agcccgaggc cgagctcaag ccggtggtgg ccgagggctc gggcgaccgc
     1621 gagcggctcg aggcccttgt caacttctac ctggagttca ccgcctacgt cgattcgctg
     1681 tacacgaggt tcgtcaccaa gaccgagctc gacttccccg agctctccaa ccgcatcaag
     1741 gccctctacg gcctgatcgt ggagaaccgc cgtttcctcc ttcgggccca gtcccaggcc
     1801 cccgcccaca agaactatct ggtctcacac gccgtcaggt cggcggtctt ctccatcatc
     1861 ctcgggaacg ccctcaagct cccgcccttc aggctcatcg agctcggggc cgccgcggtc
     1921 ctccacgaga tcggcatgat caggctgccc tcccacctct acatggccgg gcgccagctc
     1981 tcgccggtcg agcgcaagtc catcaccacc caccccgtcc tcggctacaa cctcctcaag
     2041 gagaggcagg tccccctcgc ggtctgcctg gcggccctcg aacaccacga gcgtctgaac
     2101 ggggggggct acccccgcgg catcacgggg gacaagatat ccctgtactc gcggataatc
     2161 atggtggcct gctcctatga cgccgtcacc gccgaccgtc ccttcaagcc ggcgagggac
     2221 gggtattcgg gcatggtcga catcctccgg aacgagggca aacagtacga cgagaacgtg
     2281 atccgcgccc tcgtcttcag cctctcgatc ttcccgatcg gcacctacgt catgctctcg
     2341 aacggcaagg gcggcctcgt cgtcgacgtg aaccccgagg
//
LOCUS       DASSQV010000403         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_716421, whole genome shotgun sequence.
ACCESSION   DASSQV010000403 DASSQV010000000
VERSION     DASSQV010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716421"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFL04_07000"
     CDS             <1..424
                     /locus_tag="VFL04_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7839492.1"
                     /translation="IPTAPSMVGNFTDGAKAHDLAASLIQAGADPILVLAGAAGTEVH
                     AQAQKAGARTLGWIEDMSPLAPKAVITSNVQDVPAMLLRAARLAREGRWEGKQYRFGM
                     AEGVQHLAPFHGGLSADQESRIKAVENDLLTGMLDASR"
     gene            411..>2249
                     /locus_tag="VFL04_07005"
     CDS             411..>2249
                     /locus_tag="VFL04_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HET7839493.1"
                     /translation="MRAAEPEATTTVAGRLRLGLIGAVLISLLVTGGVLVWIAFSQEL
                     DQVRRDQAAVARLSAEKISAYVDELQRKLGFLARIRGLTEFDRDSRASLLEGLAYQNK
                     AYEALGITDGGGRLSQGLYPGGEALAADWSGSAAYRRAVLLQEDFVGPVELEAGERLP
                     SLLLAVPVRNGENRVGGMLVARVGLKYLWAVLDENRVGRSGYSYVVDQRGFIVAQTGD
                     LPRQFSFGLLPGPRPEARPDRRGLAGPAPYQGLRGEAVIGEHASVQATNWTVVSELPL
                     REAYAPLGRLAALMAAGLALGLVCSILIGGRLALIITRPREDLTLAAIRLRHGELGTR
                     VAAGHDRELRVLAEAFNHMAQERMEVEGALRASEARYRSLFDSSMDAILLTITDGRVL
                     EANPAACSLFGRSLEEIQREGRSGMVDSSDQRLALALEERARTGRFFGELSFIRINGE
                     RFPVELSSTTFDGADGGRWTLIIFRDITERKRAEEAMWRALKENQELLAELQHRVKNS
                     MNMIASMISLSAQTREEAETKAVLDELEARVRAVAELYSLLYSSASFSELRLDEYCGR
                     ICSGLAGLSDRVEIMLDMERIQIPAKHAASIGLIVTELITNALKYAF"
BASE COUNT          356 a          832 c          758 g          303 t
ORIGIN      
        1 catcccaacc gcgccttcca tggtgggcaa cttcaccgac ggggcgaagg cgcatgacct
       61 ggccgcctcc ctcatccagg ccggcgccga ccccatcctc gtcctcgccg gggcggccgg
      121 cacggaagtc cacgcccagg cccagaaggc cggcgcccgc accctcggct ggatcgagga
      181 catgagcccg ctggccccca aggccgtcat cacgagcaac gtccaggacg tccccgccat
      241 gctcctgcgc gccgcgaggc tcgcgaggga gggcaggtgg gagggcaagc agtaccgctt
      301 cggcatggcc gagggggtcc agcacctcgc gcccttccac ggcggactca gcgccgacca
      361 ggagagccgg atcaaggccg tggagaacga cctcctgacc gggatgctcg atgcgagccg
      421 ctgagcccga ggccaccacc accgtcgccg gccggctcag actcggcctg atcggagccg
      481 tcctcatcag cctcctcgtc accggcgggg tcctcgtgtg gatagccttc agccaggagc
      541 tggaccaggt cagacgcgac caggccgccg tggcccggct gtccgccgag aagatatcgg
      601 cctacgtcga cgagctccag cgcaagctcg gcttcctcgc gcggatccgg ggcctcaccg
      661 agttcgaccg ggacagcagg gcgagcctcc tcgagggcct cgcctaccag aacaaggcct
      721 acgaggccct cggcatcacc gacggaggcg ggcggctcag ccagggcctc taccccgggg
      781 gcgaggccct ggcggccgac tggtcgggca gcgccgccta ccgccgcgcc gtcctcctgc
      841 aggaggactt cgtggggccg gtcgagcttg aggcggggga gcgcctgccg agcctcctcc
      901 tcgcggtgcc cgtgcggaac ggggagaacc gggtgggcgg catgctcgtc gcgcgcgtcg
      961 gcctcaagta cctgtgggcc gtcctcgacg agaaccgcgt gggcaggagc ggctactcct
     1021 acgtcgtcga ccagcggggc ttcatcgtcg cccagaccgg cgacctgccc cggcagttca
     1081 gcttcggcct cctccccggg ccgagacccg aggcgaggcc cgaccgacgg ggcctcgccg
     1141 gacccgcgcc ttatcagggc ctgcggggcg aggccgtgat cggcgagcac gcgagcgtcc
     1201 aggcgacgaa ctggaccgtc gtgtccgagc tgcccctgag ggaggcctac gcccccctgg
     1261 gcaggctggc ggccctcatg gcggccggcc tggccctcgg cctggtctgc tcgatcctca
     1321 tcggaggccg actcgccctc atcatcaccc ggccccgaga ggacctgacc ctggccgcca
     1381 tcaggctcag acatggcgag ctggggaccc gcgtggcggc tggccatgac agggagctcc
     1441 gggtcctggc cgaggccttc aaccacatgg cccaggagcg catggaggtc gagggcgccc
     1501 tgcgggcgag cgaagccagg taccgatcgc tattcgactc gtcgatggac gccatcctcc
     1561 tcaccatcac cgacggacgg gtcctcgagg ccaaccccgc ggcctgcagc ctcttcggcc
     1621 ggagcctgga ggagatccag cgcgagggcc gctccggcat ggtcgacagc tcggatcagc
     1681 ggctcgccct ggccctcgag gagcgggcgc ggaccggccg cttcttcggc gagctcagct
     1741 tcatccgcat caatggcgag cgcttccccg tcgagctctc gtcgaccacc ttcgacggcg
     1801 ccgacggcgg cagatggacc ttgatcatct tccgcgacat caccgagcgc aaacgggccg
     1861 aggaggcgat gtggcgggcc ctcaaggaga accaggagct cctcgccgag ctccagcacc
     1921 gggtcaagaa cagcatgaac atgattgcga gcatgatcag cctgagcgcc cagacccgcg
     1981 aggaggccga gaccaaggcg gtcctcgacg agctcgaggc gagggtcagg gccgtggccg
     2041 agctctactc cctcctctac tccagcgcct ccttctccga gctcaggctc gacgagtact
     2101 gcggccggat ctgctcgggc ctcgcggggc tctccgaccg ggtcgagatc atgctcgaca
     2161 tggagcggat ccagataccc gcgaagcacg ccgcctcgat cggcctcatc gtgaccgagc
     2221 tcatcaccaa cgcgctcaag tacgccttc
//
LOCUS       DASSQV010000404         4820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1088622, whole genome shotgun sequence.
ACCESSION   DASSQV010000404 DASSQV010000000
VERSION     DASSQV010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088622"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VFL04_07010"
     CDS             complement(<1..1017)
                     /locus_tag="VFL04_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7839494.1"
                     /translation="MRRIGVGIIGVGKAGRAQLEAILRLGFAELRAIAVRDGERARGL
                     CEEFGIARSFGDYREMLDDPGIEVLHNCTPNHQHFAINRAILESGKHLVSEKPLGLDS
                     AESLILWRLAGEGKAVNAVNFVYRHHALVQHLRGMIEAGELGTIRAVSGAYLQDWLLY
                     DSDYDWRVEAALGGASRAMADIGSHWVDLAQFLLGRNVVEVCADFATFVPRRAKPGAG
                     GPTMVEVDTEDYAAVLCRFEGGAHGSFTVSQASAGSRLGLWIQVDGSLASARWSQERA
                     HELWIGHRDRANEVIVSHPSLLNARALACCRVDGEGIERWPDAQKSMIAAVYRSILEG
                     AEPSY"
     gene            complement(1074..1472)
                     /locus_tag="VFL04_07015"
     CDS             complement(1074..1472)
                     /locus_tag="VFL04_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839495.1"
                     /translation="MRIEEYEHGGEGMSRVYQNANWMVGIKNWKSANDIANIDCLERH
                     NGTDELFVLLEGTCTLLFANEVDGNLVIEALRLKPLRVYKVPRSLWHNTVTTRATKLI
                     LVEDSATSGENSDVLALSEPLKAEARRLAG"
     gene            complement(1469..2416)
                     /locus_tag="VFL04_07020"
     CDS             complement(1469..2416)
                     /locus_tag="VFL04_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET7839496.1"
                     /translation="MSEPKSLRVAYGEALVELGATNDRMVVLDADLAHATMTSLFEAK
                     YPERFFNFGLAEANMMCAAAGFAHSGLLPFASTFALFGAGRAYEQIRNSISYVGANVK
                     LGLSHSGLSVGEDGGSHQSIEDIALMRGLPGMTVLVPCDPIQTRKAVFAAAAIEGPVY
                     IRVARPVVENLSAEDEAFVPGQAVILRDGRDVCIAATGLMVREALRAADALAAEGIQA
                     SVLNFHTIKPLDSVRVLEWARRCGAIVTVEEHSVIGGLGSAVAEVLAGQSDASFERIG
                     IQDLFGSSGKPSELFAAYGLCAENIASRCRALLRRKARP"
     gene            complement(2409..3227)
                     /locus_tag="VFL04_07025"
     CDS             complement(2409..3227)
                     /locus_tag="VFL04_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7839497.1"
                     /translation="MDKASLDEKAARLRRDILKMLYLCQSGHPGGSLSSLEILMALYY
                     GVARTDPRRPRMEDRDRIVLSKGHACPALYAVLADRGFFPREELWSLRQIGSRLQGHP
                     DMNKTPGVDASTGSLGQGISIALGMAMAAKAKKAGYRVFCVIGDGEAQEGMVWEAAMA
                     AAHYRLDNLAVLMDHNGLQIDGPNEKVMGLGDIEEKFRAFGFETSKVDGHHIGAIAGA
                     LRARAAGRPLFVLCETLKGKGVSFMEGNAGWHGKPMSADEYAAAMKELGGCADV"
     gene            complement(3247..4080)
                     /locus_tag="VFL04_07030"
     CDS             complement(3247..4080)
                     /locus_tag="VFL04_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7839498.1"
                     /translation="MKRGPGFLASLPGYLVLALLLAFLLFPFYWTFVTSIKPEGELYG
                     KAVSYWPVRPTLDSYRKLFTDFSFLRPMANSLIVASSTTLVSLAVSLLAAYAFSRFRF
                     RGRKLLMTLFLTNNMFPTVLLLIPLYAIMRKLGMLYTPGSLILSYTTFTIPFSIWLLN
                     GYLDDLPTSLEEAAMVDGANRVQAFLRIILPILMPCVVATGAYVFMTSWNEYTFAVMF
                     TNEASRTIPVALRNLVGLLGVQWGMMTAGGILTIIPVCIMFFFAQKRLIAGLTAGAEK
                     G"
     gene            complement(4077..>4820)
                     /locus_tag="VFL04_07035"
     CDS             complement(4077..>4820)
                     /locus_tag="VFL04_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7839499.1"
                     /translation="IRFVALRNYLAVLADRLFLEAVWKTLVWVVVGVGLQLVFGLILA
                     LILNRSFFGRGLVRSLCLIPWVTPGVLIGLMWRWIYDGNYGVLNDLLLRLGLIHDKIP
                     FLAIEQTAFPLVILTIVWQGIPFFAIMLLAGLQGIPEELYDAADVDGANGLQKLVRIT
                     LPSLRNTIYVTTLLRVIWVANSVDVIFNLTEGGPAYSTQTLSVYVFNKGNSLNLSYAS
                     TLAIMLALGLSLVAAMYLRSIFRAQGGRA"
BASE COUNT          807 a         1537 c         1679 g          797 t
ORIGIN      
        1 gtaggacggt tcggcgccct cgaggatgct ccggtagacg gccgctatca tggacttctg
       61 ggcgtcgggc cagcgctcga tcccttcgcc gtcgacgcgg cagcaggcca gggccctcgc
      121 gttcagcaat gagggatggg agacgatgac ctcgttggcc ctgtccctgt ggccgatcca
      181 gagttcgtgg gcccgctcct ggctccagcg ggccgaggcc aggctgccat ccacctggat
      241 ccagagcccg agcctgctcc cggcgctcgc ctgggagacg gtgaagctcc cgtgggcccc
      301 gccctcgaag cggcagagga cggcggcgta gtcctcggtg tccacctcga ccatggtcgg
      361 cccccccgcc cccggcttcg cccgccttgg gacgaaggtg gcgaaatcgg cgcagacctc
      421 gaccacattc cgcccgagga ggaactgggc gaggtcgacc caatgcgatc ctatgtcggc
      481 catcgcgcgc gaggccccgc ccagggcggc ctcgaccctc cagtcgtagt cgctgtcgta
      541 gaggagccag tcctggaggt aggcgccgct caccgcgcgt atcgtgccga gctcccccgc
      601 ctctatcatc ccccggagat gctgcacgag ggcgtggtgc cggtagacga agttgacggc
      661 gttcaccgcc ttgccctccc ccgcgagcct ccagaggatg agcgactccg cggagtcgag
      721 gcccaggggc ttctcggaaa ccaggtgctt gcccgactcg aggatcgcgc ggttgatcgc
      781 gaaatgctgg tggttgggcg tgcagttgtg gaggacctcg atgcccggat cgtcgagcat
      841 ttcccggtag tcgccgaagc tgcgggctat gccgaactcc tcgcaaagcc ctctggccct
      901 ttccccgtcc ctcaccgcga tggccctgag ctcggcgaag ccgaggcgga gtatagcctc
      961 gagctgggcc cgtccggcct tgccgacgcc tatgatcccg actccgatcc gcctcatgtc
     1021 ctgaccatcc cccttaccta gccccggcgc cgccgctgtg aagctcgcgg gccctagccc
     1081 gcgagccgcc tcgcctcggc cttcagcggc tcggagagcg cgaggacgtc gctgttctcg
     1141 cccgaggtgg ccgagtcctc gacgaggatg agcttggtgg ccctcgtggt taccgtgttg
     1201 tgccagaggc tccgggggac tttgtagacc ctgagcggct tcagacgcag ggcctcgatg
     1261 accaggttcc catcgacctc gttcgcgaaa aggagggtgc aggtcccttc caggaggacg
     1321 aagagctcgt cggtgccgtt gtggcgctcg agacagtcga tgttggcgat gtcgttggcg
     1381 ctcttccagt tcttgatgcc gaccatccag ttcgcgttct ggtagaccct gctcatgccc
     1441 tcgccgccgt gctcgtattc ctcgatcctc atggcctagc cttcctcctc aggagcgccc
     1501 tgcagcgcga ggctatgttc tcggcgcaga ggccgtaggc ggcgaagagc tcggacggct
     1561 tgcccgagct cccgaagagg tcctggatgc cgatccgctc gaagctcgcg tcggactgcc
     1621 cggccaagac ctcggcgacg gccgagccga gcccgccgat caccgagtgc tcctccacgg
     1681 tgacgatggc cccgcagcgg cgggcccatt ccagcacccg caccgagtcc aggggcttga
     1741 tcgtgtggaa gttcaggacc gaggcctgga tgccctcggc ggcgagggca tcggcggccc
     1801 tgagggcctc cctcaccatg aggcccgtcg ccgcgatgca gacatcccgg ccatcgcgga
     1861 gtatgacggc ctggcccggc acgaaggcct cgtcctcggc actgaggttc tctacgacgg
     1921 gtctcgcgac gcggatgtac acgggcccct cgatggctgc ggcggcgaag acggccttcc
     1981 tcgtctggat ggggtcgcag ggcacgagga ccgtcatgcc ggggaggccg cgcatgaggg
     2041 cgatgtcctc gatgctctgg tggctgcccc cgtcctcccc gaccgagagg cccgagtggg
     2101 agaggccgag cttcacgttg gcgccgacgt agcttatcga gttgcggatc tgctcgtagg
     2161 ccctgccggc gccgaaaagg gcgaaggtgc tcgcgaaggg caggaggccc gaatgcgcaa
     2221 agccggcggc ggcgcacatc atgttcgcct cggcgaggcc gaagttgaag aagcgctcgg
     2281 ggtacttcgc ctcgaacagc gaggtcatcg tggcgtgggc gaggtcggcg tcgaggacga
     2341 ccatcctgtc gttggtggcg ccgagctcga ccagggcctc gccgtaggcg actcggagtg
     2401 acttgggctc agacatcggc gcaacctccc agctccttca tcgcggcggc gtactcgtcg
     2461 gcgctcatcg gcttgccgtg ccagccggca ttgccctcca tgaaggacac gcccttgccc
     2521 ttcagggtct cgcagaggac gaaaagcggc ctcccggccg cccttgccct cagggcgccg
     2581 gctatggcgc cgatgtggtg gccgtcgacc ttcgatgtct cgaagccgaa tgcccggaac
     2641 ttctcctcga tgtcgccgag gcccatgacc ttctcgttgg gcccgtcgat ctggaggccg
     2701 ttgtggtcca tgagcacggc gagattgtcg agcctgtagt gggcggcggc catcgccgcc
     2761 tcccagacca tgccctcctg ggcctctccg tcgccgatca cgcagaagac cctgtagccg
     2821 gccttcttgg ccttagccgc catcgccatg ccgagggcga tcgagatgcc ctggccgagc
     2881 gagcccgtgc tcgcgtcgac cccgggggtc ttgttcatgt cgggatggcc ctggagacgg
     2941 ctgccgatct ggcggaggct ccagagctcc tcgcgcggga agaagccccg gtcggccagg
     3001 acggcgtaca gggccgggca ggcgtggccc ttgctcagga ctatgcggtc cctgtcctcc
     3061 atccgaggcc ttcgcgggtc ggtcctcgcg accccgtagt acagggccat cagaatctcg
     3121 aggctcgaga gcgagccccc cggatgcccc gactggcaga ggtagagcat cttcaggatg
     3181 tcccggcgca gacgcgcggc cttttcgtcg agtgaagcct tgtccatggg cacagctcct
     3241 tatggatcag cccttctcgg cccccgcggt gaggcccgcg atcagccgct tctgggcgaa
     3301 gaagaacatg atgcagacgg gaatgatggt gaggatgccc ccggcggtca tcatccccca
     3361 ctggaccccg aggaggccga cgaggttccg gagcgccacg gggatcgtcc tcgaggcctc
     3421 attcgtgaac atcaccgcga aggtgtactc gttccatgag gtcatgaaga cgtaggcccc
     3481 cgtcgccacc acgcagggca tgaggatcgg gaggatgatc ctgaggaagg cctggaccct
     3541 gttggccccg tccaccatcg ccgcctcctc gagcgaggtc ggcaggtcgt cgaggtagcc
     3601 gttcaggagc cagatcgaga aggggatggt gaaggtcgta taggagagga tgagggagcc
     3661 cggggtgtag agcatcccca gcttcctcat gatggcgtac aggggaatga ggaggaggac
     3721 ggtggggaac atgttgttgg tgaggaagag ggtcatgagc agcttcctgc ccctgaagcg
     3781 gaagcgcgag aaggcgtagg ccgcgaggag ggacaccgcg agggacacga gggtggtgga
     3841 ggaggccacg atgaggctgt tggccatggg cctgaggaag ctgaaatcgg tgaagagctt
     3901 gcggtaggag tccagggtcg gccgcacggg ccagtagctg accgccttgc cgtagagctc
     3961 cccttcgggc tttatcgagg tgacgaaggt ccagtagaag ggaaagagga ggaaggccag
     4021 gagcagggcg agcacgaggt agcccggcag ggaggcgagg aaacccggcc ccctcttcat
     4081 gccctgcccc cctgggccct gaagatgctg cggagataca tcgccgcgac gagcgaaagc
     4141 ccgagggcga gcatgatcgc gagggtcgag gcgtagctca ggttgagcga gttgcccttg
     4201 ttgaagacgt agacgctcag ggtctgggtg gaataggcgg ggccgccctc ggtgaggttg
     4261 aagatcacgt cgacggagtt ggcgacccag atcacgcgca ggagggtggt gacgtagatg
     4321 gtgttccgca gcgatggaag cgttatgcgc acgagcttct gcagcccgtt ggccccgtcg
     4381 acgtcggcgg cgtcgtagag ctcctcgggg atgccctgga ggccggccag gagcatgatc
     4441 gcgaagaagg ggatgccctg ccagacgatg gtgaggatca ccagggggaa ggcggtctgc
     4501 tcgatggcta agaagggtat cttgtcgtgg atgaggccga gacgcagcag gaggtcgttg
     4561 agcaccccgt agttcccgtc gtagatccac ctccacatga gcccgatgag cacccccggc
     4621 gttacccagg gtatcagaca gagcgagcgg accaggcccc ggccgaagaa gctgcggttg
     4681 aggatgagcg ccaggatgag gccgaagacg agctgaaggc cgaccccgac gacgacccag
     4741 accagggtct tccagaccgc ctcaaggaag agccggtcgg cgagcacggc gaggtagttc
     4801 cggagggcga cgaacctgat
//
LOCUS       DASSQV010000405         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1088807, whole genome shotgun sequence.
ACCESSION   DASSQV010000405 DASSQV010000000
VERSION     DASSQV010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088807"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1946)
                     /locus_tag="VFL04_07040"
     CDS             complement(<1..1946)
                     /locus_tag="VFL04_07040"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HET7839500.1"
                     /translation="MSGEQFFPEWKTFLGEAGEAGAPVRQVAKRSGEVQRYDRKKIAA
                     AVSRAFSAALGPQAGAGPVVAPRVERVVALVEERLGALMAGRHPNSIPAIEEIQDLVE
                     TALIEAGEAGTAKAYILYRARHEAMRDAGRLMLDINSTMDGYLSQADWRVKENANVNY
                     SLGGLILHNSGTITANYWLRNIYPEEIARAHREADFHIHDLSMFSGYCAGWSLRQLIA
                     EGLGGVPDKISSKPAKRLSTLVQQMVNFLGVLQNEWAGAQALSSFDTYLAPFIKKDAL
                     DEAEVRQCIQSFLFGVNTPSRWGSQAPFTNITLDWTCPSDLRDRQAVAGGAELAFTYG
                     DCQAEMDLLNRIFIELMLEGDSEGRGFQYPIPTYNITRDFEWDSPNARLLFEMTAKYG
                     TPYFQNFVNSDLDPGDVRSMCCRLQLDKRELRKRGGGLFGSDELTGSVGVVTLNLPRI
                     GYLARGRGGRAARDDFFSRLDALCDLAAKSLGIKRKVVTRLLEGGLFPYTRRYLGSFD
                     NHFSTIGLVGMHECCLNFLGCGIETEEGKAFALEVLSHMRKRLQDYQVSTGVLFNLEA
                     TPAESTSYRLAKHDKEIWPDIICSGSPGGAAEAARPFYTNSSQLPVDLTEDLFDALDQ
                     QEELQASYTGGTVFHVFLGEAPED"
     gene            complement(1946..>2384)
                     /gene="nrdR"
                     /locus_tag="VFL04_07045"
     CDS             complement(1946..>2384)
                     /gene="nrdR"
                     /locus_tag="VFL04_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET7839501.1"
                     /translation="RSLANGESIRRRRECLSCGLRFTSYERIEEKPLMVVKRDGRREP
                     FERLKLERGVIRALEKRPVSQLSIENLVNEIEDEAALMGKAADEIPSVELGEMVLRRL
                     YALDKVAYVRFASVYRKFETMDEFIREIERLERRSSAPAEGGS"
BASE COUNT          350 a          774 c          851 g          409 t
ORIGIN      
        1 cagtcctcgg gcgcctcgcc caggaagacg tggaagaccg tgcccccggt gtaggaggcc
       61 tggagctcct cctgctggtc cagggcgtcg aagaggtcct cggtgaggtc gacggggagc
      121 tggctcgagt tcgtgtagaa gggccgcgcg gcctcagccg ccccacccgg gctgcccgag
      181 cagatgatgt cgggccagat ctccttgtcg tgcttcgcga ggcggtagct cgtggactcg
      241 gccggggtgg cctcgaggtt gaagaggacc ccggtgctga cctggtagtc ctggagccgc
      301 tttcgcatgt gggagaggac ctcgagggcg aaggccttcc cctcctcggt ctcgatgccg
      361 cagccgagga aattgaggca gcactcgtgc atgcccacga ggccgatcgt gctgaagtgg
      421 ttgtcgaagc tcccgaggta gcgcctcgtg taggggaaga gcccgccctc gaggagcctg
      481 gtcacgacct tgcgcttgat gccgagggac ttcgcggcga ggtcgcagag ggcgtcgagg
      541 cgggagaaga agtcgtcgcg cgcggcccgt ccgccccgac ccctcgccag gtagccgatc
      601 ctcggcaggt tgagcgtgac gacccccacc gagccggtga gctcgtcgga gccgaagagc
      661 ccgcccccgc gtttgcgcag ctcgcgcttg tcgagctgga ggcggcagca catcgagcgc
      721 acatcgccgg ggtcgaggtc ggagttcacg aagttctgga aatagggcgt gccgtacttg
      781 gccgtcatct cgaagaggag ccgcgcgttg ggcgaatccc actcgaagtc gcgggtgatg
      841 ttgtaggtgg ggatggggta ctggaagccc ctgccctcgg agtcgccctc gagcatgagc
      901 tcgatgaaga tccggttcag cagatccatc tcggcctggc agtccccgta ggtgaaggcc
      961 agctcggccc cgccggcgac ggcctgcctg tcccggaggt cgctcgggca ggtccagtcc
     1021 agggtgatgt tggtgaaggg ggcctggctc ccccagcggc tcggcgtgtt caccccgaag
     1081 aggaagctct ggatgcactg gcggacctcg gcctcgtcca gggcgtcctt cttgatgaag
     1141 ggcgcgaggt aggtgtcgaa ggaggacagg gcctgggccc ccgcccattc gttctggagg
     1201 acgccgagga agttgaccat ctgctggacc agggtggaga gccgcttggc cggcttcgag
     1261 ctgatcttgt ccgggacccc gcccaggccc tccgcgatga gctggcgcag cgaccagccc
     1321 gcgcagtagc ccgagaacat cgagaggtcg tggatgtgga agtcggcctc cctgtgggcc
     1381 ctggctatct cctcggggta gatgttccgc agccagtagt tcgcggtgat cgtgcccgag
     1441 ttgtggagga tgaggccgcc gagcgagtag ttgacgttgg cgttctcctt caccctccag
     1501 tcggcctggg agaggtagcc gtccatcgtc gagttgatgt ccagcatgag cctgccggcg
     1561 tcgcgcatcg cctcgtggcg ggcgcggtag aggatgtagg ccttggccgt ccccgcctcc
     1621 cccgcctcga tgagggccgt ctcgacgagg tcctggatct cctcgatcgc ggggatggag
     1681 ttggggtggc ggccagccat gagggcgccg agcctctcct caacgagggc cacgacccgc
     1741 tccacccgcg gggcgaccac ggggccggct ccggcctgcg gccccagggc ggccgagaag
     1801 gcccgggaca cggcggccgc gatcttcttc cggtcgtagc gctgcacctc gcccgagcgc
     1861 ttggccacct ggcgcacggg ggcgcccgcc tcgccggcct cgccgaggaa ggtcttccac
     1921 tcggggaaga actgctctcc agacatcacg agcctccctc ggccggggcc gagctcctcc
     1981 gctcgagcct ctcgatctcg cgtatgaact cgtccatggt ctcgaacttg cggtacaccg
     2041 aggcgaagcg gacataggcg accttgtcca gggcgtagag gcgtctgagc accatctcgc
     2101 cgagctcgac gctcggtatc tcgtcggcgg ccttgcccat gagggcggcc tcgtcctcga
     2161 tctcgttgac gaggttctcg atgctgagct gggagaccgg ccgcttctcg agggcgcgga
     2221 tgacgccccg ttcgagcttg agcctctcga agggctcgcg gcgcccgtcg cgcttgacga
     2281 ccatgagggg cttctcctcg atccgctcgt agctcgtgaa gcgcaggccg caggacaggc
     2341 actcccgcct gcgcctgatg ctctccccgt tcgcgaggga gcgg
//
LOCUS       DASSQV010000406         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_593595, whole genome shotgun sequence.
ACCESSION   DASSQV010000406 DASSQV010000000
VERSION     DASSQV010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593595"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2051)
                     /gene="ftsH"
                     /locus_tag="VFL04_07050"
     CDS             complement(<1..2051)
                     /gene="ftsH"
                     /locus_tag="VFL04_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7839502.1"
                     /translation="MARKKGGWSKKPTSTPPKGGEKPPLVPPNFGGFQFRFSMGYIIV
                     MIVAVSLFNYFLFQADDSVVPYSVFKQKIADGEIKRVEMDTTYFTGYPDAAKAPQPTL
                     SPGTRNPAVVKTVPVADPSFTTLLDQKGVVYSASPRESNAVLSFVLNWILPLAFMFFL
                     WRVVFKRIGNVNNNVLAFGQNKATVVAEGDVRTRFADVAGVDEAKEELVEVVDFLRNP
                     KKYTDIGGKIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFRMSGAEFVEMFVGVGAAR
                     VRDLFKQAREKAPCIVFIDELDAIGKSRLTGAVGGNDEREQTLNQLLVEMDGFDATSG
                     LIILAATNRPDVLDPALLRPGRFDRQVLVDRPDLIGREAILGIHAKAVKLDPGVDLSK
                     VARGTPGFVGADLANLVNEAALLAVRSGRKKVIQHDFDAAIEKIVAGLEKKSRVMNPR
                     ERRIIAVHETGHALTAAFTKGADPVRKISIVPRGFGALGYTLQLPTEDRYVVAEEELL
                     GQIDVLLGGRAAEETVFGRISTGASNDLTRATDIARKMITDYGMSERFRHVALTKRGG
                     MFGGPMAEPAMGREYSEDTQKYIDEEIARIVAARYDQVIALLASKRNILDQVAERLLE
                     KEVIEDAEFGELAGLEPPPVQAGIAQGKAGDPRAKALPDSEPVAVPSPPLHPLPGAAA
                     SG"
     gene            2304..2570
                     /locus_tag="VFL04_07055"
     CDS             2304..2570
                     /locus_tag="VFL04_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET7839503.1"
                     /translation="MKVKPLVDRVLIKIQEGESKSAGGIIIPQTAQEKTQTGVVIAVG
                     TDKDVIKVKEGDKVMYDKYAGTQIKIEGTEHLIVKMQDILAIIE"
     gene            complement(2646..3092)
                     /locus_tag="VFL04_07060"
     CDS             complement(2646..3092)
                     /locus_tag="VFL04_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7839504.1"
                     /translation="MDLKSVLGRDSVALHLKGKTKEEIIDELLAVAGRSGKVTDLAGA
                     RKAVLERERRMSTGMKNGIAIPHGKTDTVKDLVACIGVSDTAVDFEALDRQPCRIFIM
                     TLSPADKTGPHLQFLAEVSLLFRSDEKRAAILAAESPEAVLAVLTQ"
BASE COUNT          504 a         1036 c         1120 g          588 t
ORIGIN      
        1 ccgcttgcgg cggcaccggg aaggggatgg aggggggggc ttggcaccgc cacgggttcg
       61 gaatcgggca gggccttggc cctcgggtcc ccggccttgc cttgtgcgat cccggcttgg
      121 acggggggcg gctcgagccc cgcgagctcc ccgaactcgg cgtcctctat cacttccttc
      181 tcaaggaggc gctcggccac ctggtcgagg atgttccgct tggaggcgag gagggcgatg
      241 acctggtcgt agcgcgccgc cacgatccgg gctatctcct cgtctatgta cttctgcgtg
      301 tcctcgctgt attccctgcc catcgccggc tcggccatgg gcccgccgaa catcccgccg
      361 cgcttggtca gggcgacatg gcggaaacgc tccgacatgc cgtagtcggt gatcatcttg
      421 cgggcgatgt cggtggcgcg ggtgaggtcg ttggaggcgc ccgtggagat ccggccgaag
      481 accgtctcct cggccgccct gccgccgagg aggacgtcga tctggccaag gagctcctcc
      541 tcggccacga catagcggtc ctcggtgggg agctggaggg tgtagcccag ggcgccgaag
      601 ccccggggca cgatggagat cttgcgcacg ggatcggcgc ccttggtgaa ggccgccgtg
      661 agggcgtggc ccgtctcgtg caccgcgatg atcctgcgct cgcgggggtt catgaccctg
      721 ctcttcttct cgagcccggc gactatcttc tcgatcgcgg cgtcgaagtc gtgctggatg
      781 accttcttcc tgcccgagcg cacggcgagg agggcagcct cgttcacgag gttggcgagg
      841 tcggcgccga cgaagcccgg ggtgcccctc gccaccttcg agaggtcgac gcccgggtcg
      901 agcttgacgg ccttcgcgtg gatgccgagg atggcctccc gcccgatgag gtcgggcctg
      961 tccaccagga cctgcctgtc gaagcgaccg ggcctgagga gggcggggtc gaggacgtcg
     1021 gggcggttgg tggcggcgag gatgatgagg ccgctcgtcg cgtcgaagcc gtccatctcg
     1081 acgaggagct ggttgagggt ctgctcgcgc tcatcgttcc cgcccacggc cccggtgagc
     1141 cgggacttgc cgatggcgtc cagctcgtcg atgaatacga tgcagggcgc cttctccctc
     1201 gcctgtttga agaggtccct gaccctcgcg gcgccgacgc ccacgaacat ctcgacgaac
     1261 tcggccccgc tcatgcggaa gaagggcacg ccggcctcgc cggccactgc cctcgcgagg
     1321 agggtcttgc cggttccggg cggccccaca aggaggacgc ccttggggat cttgccgccg
     1381 atgtcggtgt acttcttcgg gttcctgagg aagtccacga cctcgaccag ctcttccttc
     1441 gcttcgtcca ccccggccac atcggcgaag cgggtccgca cgtcgccctc ggcgacgacg
     1501 gtggccttgt tctggccgaa tgcgaggacg ttgttgttga cgttgccgat ccgcttgaac
     1561 accaccctcc aaaggaagaa catgaaggcg agggggagga tccagttcag cacgaaggaa
     1621 aggacggcgt tgctctcccg ggggctcgcg gagtacacga cgcccttctg gtcgaggagg
     1681 gtggtgaagc tcgggtcggc gacggggacg gtcttgacca cggcggggtt ccgggtgccg
     1741 gggctcaggg tgggctgggg ggccttggcc gcgtccgggt agcccgtgaa gtaggtcgtg
     1801 tccatctcga cccgctttat ctcgccatcg gctatcttct gcttgaacac gctgtagggg
     1861 accaccgaat cgtcggcctg gaagaggaag taattgaaaa ggctgaccgc gacgatcatc
     1921 acgatgatgt agcccatgga gaagcggaac tggaagccac cgaagttcgg gggcacaagc
     1981 gggggtttct cgccgccctt cggtggtgtc gaggtgggtt tcttgctcca gccgcccttt
     2041 ttgcgcgcca ctatggtctc ccgttccgcg tcgtggaggt ccgacgcccg ccctgatggg
     2101 cccttgcggt cttgattggc cgccttccga tctaaaggta atcattccgg ctggccaagg
     2161 ctacggaggc ctccccggct cggacgcgcc ccgggacccg gcggagcggt ggggacccac
     2221 tgccttgacc cctcgcgcgg ctttccgtat atttctcctg cataaaccat taccccccac
     2281 atgacactca ggaggattag cgtatgaaag tgaaacccct cgtcgaccgc gtgctcatca
     2341 agatccagga aggcgagagc aagtccgctg gcgggatcat catcccccag acggcccagg
     2401 agaagaccca gaccggcgtc gtgatcgcgg tcggcaccga caaggacgtg atcaaggtca
     2461 aggaaggcga caaggtcatg tacgacaagt acgccggcac ccagatcaag atcgagggca
     2521 ccgagcacct catcgtcaag atgcaggaca tcctcgcgat catcgagtag ccgcgctctc
     2581 gagcgcctgc aagcgaaggg ccgcccttgt ggggcggccc tcgtctttgt gctgggcttg
     2641 cggatctact gggtgaggac ggcgagcacg gcctcggggc tctcggcggc gaggatggcg
     2701 gcgcgcttct cgtcgctgcg gaagagcagg ctgacctcgg cgaggaactg gaggtggggg
     2761 ccagtcttgt cggcgggcga cagggtcatg atgaagatcc ggcagggctg gcggtcaagg
     2821 gcctcgaaat cgacggccgt gtccgagacg ccgatgcagg ccacgaggtc cttcaccgtg
     2881 tccgtctttc cgtgcgggat ggcgatgccg ttcttcatgc ccgtgctcat gcggcgctcg
     2941 cgctcgagga cggccttcct cgccccggcg aggtcggtga ccttgcccga gcgtcccgcc
     3001 acggcgagca actcgtctat gatctcttcc ttggtcttgc ccttcaggtg cagggcgacg
     3061 ctatcgcggc ccaatacgct cttgaggtcc ataccacatt ctacggcccc cgcgagcgag
     3121 gcgtcaagca tggccgcaca gacgcgcttg tgggggcccg ggaacccgtc cagagcatcg
     3181 gcagggcgtc tagatgtgga acctcggctc cttgtcctgg acgtggcccg atttcctcga
     3241 gagcacga
//
LOCUS       DASSQV010000407         4006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_96033, whole genome shotgun sequence.
ACCESSION   DASSQV010000407 DASSQV010000000
VERSION     DASSQV010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96033"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1044
                     /locus_tag="VFL04_07065"
     CDS             127..1044
                     /locus_tag="VFL04_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET7839505.1"
                     /translation="MELRHFRYFLAVADHLSFIKAAERLHISQPPLSRQIRELEREIG
                     TPLFYRGKHIRLTKAGLHLQDQARRILDLTGAACRDARLLGEERASKVNIGCVSFVLS
                     TVLLPLFELIRDRIPGLTLELRVMSTEAQVQALKSGTIDIGFVRSWVRVEDFAFEPLV
                     LERLYLIYPSGFESAGGTALPLSALAEQPFVGISQAAAPGLAERIEGICLQTGFTPSV
                     AYECNDAHAIERLVAAGLGWSIVPDLALERDAPAGVRSIQLPETITLGICYRNESHPE
                     TIDEILKLARSHFAGLEDPAVISAQAAAP"
     gene            1194..3671
                     /locus_tag="VFL04_07070"
     CDS             1194..3671
                     /locus_tag="VFL04_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7839506.1"
                     /translation="MRKAFRFLSISWIVLIIILASCQTKPQTPPQGESSQISSIQAEA
                     AGLAPAGDPRFQTIKFAVVIGAKESAKAWTFAIVNAKGEVERSSKGDATALPDSLSWD
                     GKNNGGALSPEGNYTASLTIDYGSNLKPVTVSSKPFLLDINPPTGSFKLNPLAFTPTA
                     SGVKGPITATVEVKPALAKVESWTIEVFDPSGATIRTLSAQWPSNQASWDGKTDAGDT
                     VKTATSYTALLKVRDEFGNTGTFKGEVAVTGVPNPEASTIIAGRNRAGFSPTSTSVKN
                     SLDILLTFGSKASISSWKVEVMSASKGTVKTFSGDGTNLPEFVQWNGKDDAAQLAPEG
                     SYFATLTVDYGGNFARAVVKSRNFSLVTTAPTGSVTVDPPSVYVADVGPKKPINFTVQ
                     AKSPFAQIAKWNLSIQAPDGTTVAVFNANWPNNKAAWDGAMLVGSSLAPATLYKAVAT
                     VEDEYGNIGTLEGSLNTETLLAATEPTTITARQRGFAPKGDGSFPAIDLDLTMGNAAA
                     VKTWKVDLIGPDGTIRDTLTGTGAKLPVSVVWDGTMPSRALAPEGYYTAQLSVDYGNA
                     YAPATAKSAAFLLDITPPSGSIELSSKIFSPDGTPEGEKVTITMNAGSSFAKIVGWDL
                     SILDPELGLFASMKGVWPKNTVVWDGTGANGELVESASDYSVVAKIRDEFGNIGSLST
                     KLVTDILAIKTARGYRIRVPSIVFKPYTSDYRDVPPDRAARNITTLDLLAGTLKVFPY
                     YKIRLEGHAVMINWDDRIKGQAEQSEVLIPLSVDRAEAIKAALVERGVVGSRFAAQGL
                     GANDPVVPDSDLLNRWKNRRVEFFLEK"
     gene            complement(3734..>4006)
                     /locus_tag="VFL04_07075"
     CDS             complement(3734..>4006)
                     /locus_tag="VFL04_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HET7839507.1"
                     /translation="KPDFVFFGEGIPPDAYAAAFGAAEAAGLCLIVGSTGLVYPAAEI
                     PRAVKRGGGMIIEVDPGQTEFTRTLTDIHVRLGAVEALEAIDAALG"
BASE COUNT          732 a         1453 c         1210 g          611 t
ORIGIN      
        1 ctgaggccgc ggcttccgga caagtttggc gccatccttc ggccccgcgc ccgcgcggac
       61 cggccgcgga gcccgtgtct gggcccgcaa cttgcgcccc gctgggtttg gcgataccct
      121 ttgggcatgg aactacggca ctttcggtat ttccttgctg tcgctgacca tcttagcttc
      181 atcaaggccg ccgaaaggct ccacatatcc caacccccct tgagcaggca gatccgcgaa
      241 ctcgagcgcg agatcgggac tccactcttc taccgcggaa agcacatccg cctcaccaag
      301 gctgggctcc acctgcagga ccaggcccgc cggatcctcg acctgacggg ggccgcgtgc
      361 agggacgccc ggctcctcgg cgaggagagg gcatcgaagg ttaacatcgg ctgcgtgagc
      421 ttcgtcctct cgaccgtcct cctgcccctc ttcgagctca tacgggaccg catcccgggc
      481 ctcaccctcg agctccgcgt catgtccacc gaggcccagg tccaggcgct caagagcggg
      541 accatcgaca tcggctttgt gcgttcctgg gtcagggtcg aggatttcgc ctttgagccc
      601 ctcgtcctcg agaggctcta cctcatctac ccgagcggct tcgagtcggc cggcgggacg
      661 gctctccccc tgtctgccct ggccgagcag cccttcgtcg gcatctccca ggcggcggcc
      721 cccggactgg ccgagcgcat cgaggggatc tgtctgcaga cgggcttcac gccttcggtc
      781 gcctatgagt gcaacgacgc ccacgcgatc gaacgcctcg tggcggccgg cctcggctgg
      841 tccatagtcc ccgacctcgc cctcgagcgc gacgcccccg caggggtgcg ctcgatccaa
      901 ttgcccgaaa ccatcaccct cggcatttgt tataggaatg agagccatcc ggaaaccatc
      961 gacgagatcc tcaagctcgc gagaagccat tttgccggcc ttgaggatcc ggctgtcatt
     1021 tccgcccagg ccgccgcgcc ctgagccccg gattcccggt cacgacgccg ttctgccctg
     1081 cccctcgcga agcccagcac cgcggcgtat aggacagtgc ccaaaaggta ttggacacac
     1141 gatgcccggg acaggaacat ttggttcatg atgagcttga ggaggatcac acaatgcgaa
     1201 aagccttcag gttcctatcg atctcctgga tcgttctgat aatcatcctc gcatcctgcc
     1261 agacgaagcc ccagactccg ccgcagggcg agtcgagcca gatctcctcg atccaggccg
     1321 aggccgccgg gctcgcgccc gccggcgatc cccgcttcca gacgataaag ttcgccgtgg
     1381 tcatcggggc caaggaatcg gcgaaggcct ggaccttcgc gatcgtcaac gccaagggcg
     1441 aggtcgagcg ctcgagcaag ggcgacgcga ccgccctccc cgacagcctc tcctgggacg
     1501 gcaagaacaa cggcggggcc ctctcgcccg aaggcaacta caccgccagc ctcacgatcg
     1561 actacggcag caatctcaag ccggtcaccg tctcctccaa gccctttctc ctcgacataa
     1621 atccgcccac cggcagcttc aagctcaacc ccctcgcctt cacgccgacg gcgtccgggg
     1681 tcaagggccc gatcacggcg acggtcgagg tcaagccggc cctcgcgaag gtcgagagct
     1741 ggaccatcga ggtcttcgac ccgagcgggg ccacgatccg caccctatcg gcccagtggc
     1801 cctccaacca ggcgagctgg gacggcaaga ccgacgcggg ggacacggtc aagacggcga
     1861 cgagctacac ggccctgctc aaggtgaggg acgagttcgg caacacgggg accttcaagg
     1921 gcgaggtcgc ggtgacgggc gtcccgaacc ccgaggccag caccataata gcggggcgca
     1981 accgcgccgg attctccccg acgagcacga gcgtgaagaa cagcctggac atcctcctga
     2041 ccttcgggtc caaggcctcg atcagctcct ggaaggtgga ggtgatgagc gcgtccaagg
     2101 gcacggtcaa gaccttctcg ggggacggga ccaatctgcc cgagttcgtc cagtggaacg
     2161 gcaaggacga cgccgcgcaa ctcgcccccg aaggctcata cttcgccacc ctcacggtgg
     2221 actacggcgg caacttcgct agggccgtgg taaagagcag gaacttctcc cttgtcacga
     2281 cggcgcccac aggatcggtc accgtcgatc ccccctcggt atacgtcgcc gacgtcgggc
     2341 ccaagaagcc catcaacttc accgtccagg ccaagtcgcc cttcgcccag atcgccaagt
     2401 ggaacctcag catacaggcc cccgacggca cgaccgtcgc cgtcttcaac gcgaactggc
     2461 ccaacaacaa ggcagcctgg gacggcgcga tgctcgtcgg gagctccctc gcccccgcga
     2521 ccctctacaa ggccgtcgcg acggtggagg acgagtacgg caacatcggg accctcgaag
     2581 gaagcctgaa caccgagacc ctgctcgcgg ccaccgagcc gacgacgatc acggcgcgac
     2641 agcgcggctt cgcccccaag ggcgacggct ccttccccgc catcgacctc gacctcacca
     2701 tgggcaatgc cgccgcggtc aagacctgga aggtcgacct cattggcccc gatgggacca
     2761 tccgcgacac cctgaccggc accggcgcca agctccccgt cagcgtggta tgggacggca
     2821 cgatgccgag ccgggccctc gcccccgagg gctactacac cgcccagctt tcggtcgact
     2881 acggcaacgc ctacgccccc gcgaccgcga agtcggccgc cttcctcctc gacatcacgc
     2941 ccccctcagg ctcgatcgag ctctcgagca agatcttctc gcccgacggc acgcccgagg
     3001 gagagaaggt cacgataacg atgaacgcgg gctcctcctt cgcgaagatc gtgggctggg
     3061 acctgagcat cctggatccc gagctgggcc tcttcgccag catgaagggg gtctggccca
     3121 agaacaccgt cgtctgggac gggacggggg ccaacggcga gctcgtcgag tccgcctccg
     3181 actattcggt ggtcgccaag atccgcgacg agttcgggaa catcggcagc ctgtcgacca
     3241 agctcgtgac cgacatcctg gccatcaaga cggcgcgggg ctaccgcatc cgggtgccga
     3301 gcatcgtctt caagccctac acctccgact accgcgacgt cccccccgac agagccgccc
     3361 gcaacatcac cacactcgac ctccttgccg ggacgctcaa ggtcttcccg tactacaaga
     3421 tcaggctcga ggggcacgcc gtcatgatca actgggacga ccgcatcaag ggccaggctg
     3481 agcagtctga ggtcctgata ccgctctcgg tcgaccgggc cgaggcgatc aaggcggccc
     3541 tcgtggagcg gggcgtcgtg ggctcacgct tcgcggccca gggcctcggg gcgaacgatc
     3601 ccgtcgtgcc ggacagcgac cttctgaacc gctggaagaa ccggcgcgtg gagttcttcc
     3661 tggagaagta gaccagccat ggcggggccg cggaagcggc cccgtttctt tcctgcccgc
     3721 ggggccccgg gccctagccc agggctgcgt cgatggcctc cagggcctcg accgcgccaa
     3781 gccgaacgtg gatgtccgtc agggtcctcg tgaactccgt ctgcccggga tcaacctcaa
     3841 tgatcatccc gcctccccgc ttgaccgcgc gggggatctc ggccgctgga tagaccagac
     3901 cggtggagcc cacgatgagg cagagcccgg cggcttcggc cgccccgaag gcggctgcat
     3961 acgcatcggg cggaatcccc tcgccgaaga agacgaagtc gggctt
//
LOCUS       DASSQV010000408         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1464496, whole genome shotgun sequence.
ACCESSION   DASSQV010000408 DASSQV010000000
VERSION     DASSQV010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464496"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFL04_07080"
     CDS             complement(<1..336)
                     /locus_tag="VFL04_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7839508.1"
                     /translation="MARGRGFGPYLGPTALISLGFFTMGLMDPLYDNFVPIFLRHYIP
                     SMSLVGAIMTIDNVFALFLIPLVSIWSDRTRTGIGRRMPFIVVALPASALLFAGLPSA
                     AAASAAALLA"
     gene            complement(387..1682)
                     /locus_tag="VFL04_07085"
     CDS             complement(387..1682)
                     /locus_tag="VFL04_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HET7839509.1"
                     /translation="MAGKAKDSSSIGGKAAKKALAGLYGKGVAAQAERYSRLASLLRG
                     DLEAASLAGEAGQPRFYSAPGRTELGGNHTDHNHGQVLCAAVSLDAVACAVRLPGTRV
                     SLVSDGYADPVVLDLATLEPLPAERGKTAALVRGVARGLADRGIAPVGFAARINSTVL
                     PGSGLSSSAAIEVLLGSLMADLAGSPLSALEIAKIGQFAENEFFGKPCGLMDQTASAV
                     GGVVSIDFADPRSPKVKRLEFDFEAEGYRLVVVGTGGSHEDLTAEYAAIPGEMRAAAS
                     FLGAETLGDIDPSLLAQKGPELRAALGDRALLRAFHFMGEDARVPAMVKALKAGDLKA
                     YLKLVKKSGDSSWRLLQNISPSGAVAEQGIALALALSAEMLGAKGAWRVHGGGFAGTI
                     QAYVPAKLLAAYTELMEGYFGAGCVIPVSIRAAGAGRVL"
     gene            1816..2325
                     /locus_tag="VFL04_07090"
     CDS             1816..2325
                     /locus_tag="VFL04_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839510.1"
                     /translation="MLLKSLEEILSGTAHDVGERWLAKVRAVGNTRALNEIGDGELLS
                     VHEEVMRKLARWFELEADKNEIGAFFVMVGKEYCALGVPISELTFALNLDRKAVTEYL
                     TEGEELEGAHRIYALMEAVDKVAEFYMLSAYYMTKGYLEETFVRLRKCEGLPDEALTK
                     YFKDDFFFK"
     gene            2336..>2532
                     /locus_tag="VFL04_07095"
     CDS             2336..>2532
                     /locus_tag="VFL04_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839511.1"
                     /translation="MNHRYTSVPLGTIFGFLFAMYAGLCLSAFLSAGGQGDRAARGAA
                     AVFLVATLSLARYLVIAFFARS"
BASE COUNT          408 a          823 c          904 g          397 t
ORIGIN      
        1 ggcgaggagg gcggcggccg aggccgcggc ggcggagggg aggcccgcga agagcagggc
       61 gctcgcgggc agggccacca cgatgaaggg catgcgccgc ccgatcccgg tcctcgtgcg
      121 gtccgaccag atcgacacga gggggatgag gaagagggcg aagacgttgt cgatcgtcat
      181 gatcgcgccc acgaggctca tcgaggggat gtagtgtctg aggaagatgg gcacgaagtt
      241 gtcgtacagg gggtccatga ggcccatcgt aaagaagccg aggctgatga gggccgtggg
      301 gccgaggtag ggcccaaagc ccctgccccg cgccaccgcc actccggccg cgtccgccgg
      361 gctcgccgcc cttgcggagg ccggcttcac agcactctcc cggccccggc cgccctgatg
      421 gagacgggga tcacgcagcc ggctccgaag tagccctcca tgagctcggt gtaggcggcg
      481 aggagcttgg cggggacata ggcctggatg gttccggcga agcccccgcc gtggaccctc
      541 cacgccccct tggccccgag catctcggcg ctcagggcca gggcaagggc gatgccctgc
      601 tcggcgacgg ccccgctcgg gctgatgttc tggaggaggc gccacgagga gtcgcccgac
      661 ttcttcacga gcttgagata ggccttgagg tcgccggcct tgagggcctt gaccatggcg
      721 gggacacggg cgtcctcgcc catgaagtgg aaggcgcgca ggagggcccg gtcgccgagg
      781 gccgccctaa gctcgggccc cttctgcgca aggagggacg ggtcgatgtc cccgagggtc
      841 tcggccccga ggaaggaggc ggcggccctc atctccccgg ggatcgccgc gtactcggcg
      901 gtgaggtcct catggctccc ccccgtgccg accacgacga ggcggtagcc ctcggcctcg
      961 aagtcgaact cgaggcgctt gaccttgggc gagcgggggt cggcgaagtc gatggacact
     1021 accccgccca ccgcgctcgc cgtctggtcc atgaggccgc agggcttgcc gaagaactcg
     1081 ttctcggcga actggccgat cttggcgatc tcgagggcgc tcaaggggga gcccgcgagg
     1141 tcggccatca ggctgccgag gaggacctcg atggcggccg agctcgaaag ccctgagccg
     1201 ggcaggaccg tcgagttgat cctggcggcg aaacccaccg gggctatgcc cctgtcggcg
     1261 aggcccctgg ccacccccct gacgagggcg gcggtcttcc ccctctcggc cgggaggggc
     1321 tcgagcgtgg ccaggtcgag gacgacggga tcggcgtagc cgtcggagac gaggctcacc
     1381 ctcgtgcccg gaagcctgac ggcgcaggcc acggcgtcga gggacacggc ggcgcagagg
     1441 acctggccgt ggttatggtc ggtgtggttg ccgccgagct cggtccggcc gggggccgaa
     1501 tagaagcggg gctggccggc ctccccggcg agcgaggcgg cctcgaggtc gcccctgagc
     1561 agggaggcga ggcgggaata gcgctcggcc tgggcggcca cgcccttgcc gtagagcccg
     1621 gccagggcct ttttggccgc ctttccgcct atcgagctgg aatccttcgc cttccctgcc
     1681 attctgagct ccttgggcct gtcgttcgcc ccattgtagc gaaaccaggg ggcgggcaga
     1741 agggccatct gctgtttgac acaaatgggc caatggaata ctctaatgcc gagcgctgtt
     1801 catccaggag ccgccatgct gctcaagagc ctcgaagaga tcctctctgg cacggcccac
     1861 gacgtgggcg agcgctggct cgccaaggtc agggccgtcg ggaacaccag ggccctcaac
     1921 gagatcgggg acggggagct gctctcggtg cacgaagagg tcatgcgcaa gctcgcccgc
     1981 tggttcgagc tcgaggcaga caagaacgag atcggcgcct ttttcgtcat ggtgggcaag
     2041 gagtactgcg ccctcggcgt cccgatctcg gagctgacct tcgccctgaa cctcgaccgc
     2101 aaggccgtga ccgagtacct cacggaaggg gaggagctcg agggcgcgca tcggatctat
     2161 gccctcatgg aggcggttga caaggtcgcg gagttctata tgctgagcgc ctattatatg
     2221 accaagggct atcttgagga gaccttcgtg cgcctgagga aatgcgaggg ccttcccgac
     2281 gaggccctga ccaagtactt caaggacgac ttcttcttta aatgacgcga ggtatatgaa
     2341 ccataggtac accagcgtcc ccctcgggac gatattcggc ttcctgtttg ccatgtacgc
     2401 cggcctctgc ctctccgcct tcctctccgc tggcggtcag ggggacaggg ccgcgcgcgg
     2461 cgccgcggcg gtcttcctcg tcgctaccct cagcctagcg aggtacctcg tgatcgcctt
     2521 tttcgcccgc tc
//
LOCUS       DASSQV010000409         5283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_969230, whole genome shotgun sequence.
ACCESSION   DASSQV010000409 DASSQV010000000
VERSION     DASSQV010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969230"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VFL04_07100"
     CDS             complement(<1..996)
                     /locus_tag="VFL04_07100"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7839512.1"
                     /translation="MEQKDYRLAAIMYTDIAGFSRMMEKDEASTLELLKYHNDLIAEI
                     VERHHGKIIKTIGDALLVDFKNTVEALQSAMEVQDKLYEHNKQTPGLPLLVRIGVHLG
                     DIYFFENDALGEGINIAARLQSLARPGCICFSQDVYNLVLNKIEFRAEKLGKVSLKNI
                     TKEIHAYEITTPNVEFDPEKDKPRSGYKPGSYLDEEGEVPVGGVASSTGPLSTRSAPP
                     AAPVPPVPPIQTVAAGPDSAEGGTPTPLGERSYTEEGSRNLLNEIRRAILQDIKGMGR
                     RMTVDEALSRYSFYGVEAREVIATMAEQGLLTKSERGFGAPGGPGQGFERPGFGPQ"
     gene            complement(1048..2967)
                     /locus_tag="VFL04_07105"
     CDS             complement(1048..2967)
                     /locus_tag="VFL04_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HET7839513.1"
                     /translation="MFSPQSIALYKNRPVLVVEIRDRIEIRLDDGSSLRVRDKDLVPL
                     HPGPVREIPEAAAGGDFDTARRMLAPDSAGSGAATTSWAELSDLVFGSSGAAEAIACW
                     REAVEGSRFRLVDGHPSALDDAEAEREAGRRARKEGEAGERAAFLERAKKARAARRQK
                     PADSGASAADLPGFEPGDERFLSELEALALGKTTKSKLCAEIGVSESPEGAQTFLLAV
                     GRWDEMVNPHPSRAGCPLSAPKMAIDQGAAKGRGELGRVDLTAMESWAIDNAWSHDPD
                     DAIAWDGSSVWVHVADPASLILPGSPADIEALARGSTLYLPELTSPMLPDQALERFGL
                     GLSPTSPALSVRIELAEDASVRAVEVLASTVRVRRASYGEADALIAAGKASELAALDR
                     IADLRRERRVADGAIEIDIPEVRIKVSGRDIGIEAVPHCHSSGIVREMMLLAGEAVAR
                     WAFERRLAFPYYSQESPGEPGALANGEGLSAQFARRRLMRSGMSGPSPSAHRGLGLPF
                     YAQATSPLRRYQDLLGHMQLRAVLEGRTPLDADEVGRRCALAQAASGQTRQAERASDL
                     HWTLAWLSRNPHWQGEAVLVGTGGPGSWQAYVPALGLETRLRLGDDHGLDELVAVELS
                     RVDLVKLECSFDAQR"
     gene            3172..3429
                     /locus_tag="VFL04_07110"
     CDS             3172..3429
                     /locus_tag="VFL04_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839514.1"
                     /translation="MIALIVGIVFIAFAVFASVPGPLDWWTDVLAFLRGSLPVLAVLI
                     GLIAVFIGIADIKDRMEAKKEEAEEAKQAAAKEAAGEGKKA"
     gene            3603..4031
                     /gene="rplM"
                     /locus_tag="VFL04_07115"
     CDS             3603..4031
                     /gene="rplM"
                     /locus_tag="VFL04_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET7839515.1"
                     /translation="MKTVFVTINTAERSWYVIDAEGKSLGRVAAKVAYMLRGKNKPTY
                     TPSQETGDYIVVVNAGKVAVTGRKRQNKMYHHHTGFPGGMKNQTFNQLIERDPIQPLE
                     IAIRGMLPKGPLGRKLYKNAKIYAGPEHPHAAQAPVAIDL"
     gene            4044..4436
                     /gene="rpsI"
                     /locus_tag="VFL04_07120"
     CDS             4044..4436
                     /gene="rpsI"
                     /locus_tag="VFL04_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET7839516.1"
                     /translation="MITNLGIGTGRRKCAVARVFLREGTGKIVVNGKEVDQYFKQGAH
                     VYAVRQPFIVTAAENKYDVLIDVSGGGPNGQAGACRHGVARALAQVDPASHTALRSNG
                     FLTRDPRMVERKKYGQRGARRRFQFSKR"
     gene            4462..5094
                     /locus_tag="VFL04_07125"
     CDS             4462..5094
                     /locus_tag="VFL04_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HET7839517.1"
                     /translation="MKIESVKLGASGTATVAAGGSSFLVRLSLLGELGLEPSTLVPGA
                     ELGELEGGLLALAAEVHEAEKRGLALLARAEQSAWMLRAKLETRGLSPRAVKIALGRL
                     SASGILDDARFAAAYAAARLARRAEGPASLAAALRAKGLDGETVKAAVTAAFGQVAAP
                     EERRLALEKAAARELKRSKGDEGLARSRLRALGYKSAEIAELFGEGDGAP"
BASE COUNT          809 a         1802 c         1858 g          814 t
ORIGIN      
        1 ctgcgggccg aagcccggcc gctcgaagcc ctgacccggt cctcccgggg cgccgaagcc
       61 gcgctcgctc ttcgtgagca ggccctgctc ggccatcgtc gcgatgacct cgcgggcctc
      121 gaccccgtag aacgaatagc gggacagcgc ctcgtccacg gtcatccgcc tgcccatgcc
      181 tttgatgtcc tggaggatcg cccgcctgat ctcgttgagc aggttgcgcg aaccctcctc
      241 cgtgtagctc cgctcgccca ggggcgtggg cgtgccgccc tccgccgagt cgggcccggc
      301 ggcgacggtt tgaatgggcg gcacgggcgg cacgggagcc gccggcgggg ccgacctcgt
      361 ggagagcggc cccgtggagg aggccacgcc cccgaccggg acctcgcctt cttcgtcgag
      421 gtaggagccg ggcttgtagc ccgagcgcgg cttgtccttt tccggatcga actccacgtt
      481 aggcgtcgtt atctcgtagg catggatctc tttcgtgatg ttcttgagcg aaaccttgcc
      541 gagcttctcc gccctgaact cgatcttgtt gaggacgagg ttgtacacgt cctgcgagaa
      601 gcagatgcag cccggcctcg cgagggactg gagcctggcc gcgatgttga tgccctcgcc
      661 gagggcgtcg ttctcgaaga agtagatgtc gcccaggtgc acgccgatcc gcacgaggag
      721 ggggaggccg ggcgtctgct tgttgtgctc gtagagcttg tcctgcactt ccatggcgct
      781 ctgcagcgcc tcgaccgtgt tcttgaaatc cacgagcagg gcgtcgccta tcgtcttgat
      841 gatcttgccg tgatggcgtt cgacgatctc ggcgatgagg tcgttgtggt acttgaggag
      901 ctcgagggtg ctcgcctcgt ccttctccat catccgcgag aagcccgcga tgtccgtgta
      961 catgatggct gcgaggcggt agtccttctg ctccatggtg ggaaaataat agatgagacg
     1021 gggagccggg tcaatgacgg gtcgatttca cctttgcgcg tcgaaggagc actcgagctt
     1081 cacgagatcg acccgggaca gctccaccgc cacgagctcg tccaggccat ggtcgtcgcc
     1141 cagccggagc ctcgtctcga gcccgagggc ggggacatag gcctgccagg agccgggccc
     1201 gccggtgccg acgaggacgg cctcgccctg ccagtgggga ttccgcgaga gccaggccag
     1261 ggtccagtgg aggtcgctgg cgcgctcggc ctggcgggtc tggcccgagg cggcctgggc
     1321 gagggcgcag cgcctgccca cctcgtcggc gtcgaggggc gtcctgccct cgaggacggc
     1381 gcggagctgc atgtggccga ggaggtcctg gtagcggcgc agggggctcg tggcctgggc
     1441 gtagaagggc agcccgaggc cgcgatgcgc gctggggctc ggccccgaca tgccggagcg
     1501 catgaggcgg cggcgggcga actgggccga caggccctcg ccgttcgcga gggccccggg
     1561 ctcgccgggg gactcctggc tgtagtaggg gaaggcgagg cggcgctcga aggcccagcg
     1621 cgcgacggcc tcgccggcga ggagcatcat ctcgcgcacg atgccgctgg aatggcagtg
     1681 cggcacggcc tcgatcccga tgtcgcggcc cgagaccttg atcctcacct cggggatgtc
     1741 gatctcgatg gccccgtcgg cgacgcgccg ctcccggcgc aggtcggcga tgcggtcgag
     1801 ggccgcgagc tcgcttgcct tgcccgcggc gatgagggcg tcggcctcgc cgtaggaggc
     1861 tcggcgcacc ctcaccgtcg aggcgaggac ctcgacggcc ctgaccgagg cgtcctcggc
     1921 cagctcgatc cggacggaga gggcggggga ggtcggcgag aggccgaggc cgaagcgctc
     1981 cagggcctgg tccgggagca tgggcgaggt aagctcgggc aggtaaaggg tggagccgcg
     2041 ggcgagggcc tcgatgtcgg cgggagaacc ggggaggatc agcgaggcgg ggtcggcgac
     2101 gtggacccag accgaggacc cgtcccaggc tatcgcgtcg tcggggtcgt gggaccaggc
     2161 gttgtcgatg gcccaggatt ccatggcggt caggtcgacg cgcccgagct cgccgcgtcc
     2221 cttggcggcc ccctggtcga tggccatctt cggcgccgaa agcggacagc cggcccggct
     2281 cgggtggggg ttcaccattt cgtcccaccg gcccacggcg aggaggaagg tctgggctcc
     2341 ctcggggctt tcgctcaccc cgatctcggc gcagagcttg cttttggtgg tcttgccaag
     2401 ggccagggcc tcgagctcgg agaggaagcg ctcgtccccg ggctcgaagc cggggagatc
     2461 ggcggccgag gctccggagt ccgcgggctt ctgccgcctc gccgcccgag ccttcttcgc
     2521 ccgctcgagg aaggccgccc tctcgccggc ctcgccctcc ttgcgcgccc ggcgcccggc
     2581 ctcgcgttcg gcctcggcgt cgtcaagggc cgagggatgg ccgtccacga ggcggaaccg
     2641 cgagccctcc acggcctcgc gccagcaggc tatcgcctcg gccgcccccg agcttccgaa
     2701 cacgaggtcg gacagctcgg cccagctggt cgtggcggct ccgctcccgg ccgaatcggg
     2761 cgcgagcatc ctccgggccg tgtcgaagtc cccgccggcg gcagcctcgg ggatttcccg
     2821 cacggggccg gggtggaggg gcacaaggtc cttgtccctg accctgaggc tcgagccgtc
     2881 gtcgagcctg atctcgatcc ggtcgcggat ctcgacgacg aggacgggtc tgttcttgta
     2941 gagggcgatc gattgggggc tgaacatcgg ctctggagta tagcggggcc tcctccgctg
     3001 gacaatcctg cggcctgggc ggcgggcccg cctgcccggg ccctccccgc gttgcttgcc
     3061 ctcgaggccc gcaggtggta ttatggcctc ccgggatgcc cgaaaacggc aatgtgtccc
     3121 gttgcataag caaacatccg ttcaaggctg gagcttcagg aggaattaag catgatagca
     3181 ctcattgtag gcatcgtatt catcgccttc gccgtgttcg ccagcgtccc cggccccctc
     3241 gactggtgga ccgacgtcct cgccttcctc aggggctccc tccccgtcct ggccgtcctc
     3301 atcggcctca tcgccgtctt catcgggatc gccgacatca aggacaggat ggaggcgaag
     3361 aaggaggagg ccgaggaggc caagcaggcg gccgccaagg aagccgcggg cgaagggaag
     3421 aaggcctgaa gcccagacgg ggcgacctgg gccgcgggtg cccgagtcgg acctcggtgt
     3481 tcgccggtgc gggcaggggc gccgccgggg cggcacctgc tattgacact gcggctgcta
     3541 tgtgggaata taccgcccgc acgtttctcg gacattccac gcgaaggtga aggtatacat
     3601 caatgaagac cgtcttcgtt acgatcaaca cggctgaacg ctcctggtac gtcatcgacg
     3661 ccgaggggaa gagcctcggc agggtggccg ccaaggtcgc atacatgctc cgcggcaaga
     3721 acaagccgac ctacactccc tcacaggaga ccggcgacta catcgtcgtc gtcaatgccg
     3781 gcaaggtggc ggtgactggg cgcaagcgcc agaacaagat gtatcaccac cacaccggtt
     3841 tcccgggcgg gatgaagaac cagaccttca accagctcat cgagcgcgac ccgatccagc
     3901 cgctcgagat cgccatccgc ggcatgctcc ccaagggccc cctgggacgc aagctctaca
     3961 agaacgccaa gatctacgcc ggccccgagc atccccacgc tgctcaggct cccgtcgcca
     4021 tcgacctctg ataaggaagc aggatgatca ccaacctagg aatcggaacc ggccgccgca
     4081 agtgcgccgt cgcgcgcgtc ttcctccgcg agggcacggg caagatcgtc gtcaacggca
     4141 aggaagtcga ccagtacttc aagcagggcg cccacgtcta cgccgtcaga cagcccttca
     4201 tcgtcaccgc ggccgagaac aagtatgacg tcctcatcga cgtctccggc ggtggcccca
     4261 acggccaggc cggcgcctgc cgccacggcg tcgccagggc cctggcccag gtcgaccccg
     4321 cgagccacac cgccctccgc tccaacggct tcctcacccg cgacccgcga atggtcgagc
     4381 gcaagaagta cggccagcgc ggagcccgca ggcgcttcca gttcagcaag cgctaaacca
     4441 aggaccagca gcccaccgac catgaagatc gagtcggtga aactgggagc ctccggcacc
     4501 gcgaccgtcg cggccggggg ctcctccttt ttggtgaggc tctccctcct tggcgagctc
     4561 ggcctcgagc cttccaccct ggtcccggga gccgagctcg gggagctgga gggcgggctc
     4621 cttgccctcg cggccgaggt ccatgaggct gagaaacggg gcctcgccct gttggccagg
     4681 gccgagcagt cggcctggat gctccgcgcc aagctggaga cccgggggct gtccccgcgg
     4741 gctgtgaaga tcgccctcgg ccggctctcg gcctcgggca tcctcgatga cgcccgcttc
     4801 gccgcggcct atgcggctgc ccggctcgcc cgcagggccg aggggccggc gagcctcgcc
     4861 gctgccctca gggccaaggg cctggacggg gagaccgtga aggccgcagt gacggccgcc
     4921 ttcggccagg tcgcggcccc cgaggagcgc cgcttggccc tggagaaggc ggccgcgcgc
     4981 gagctcaaac ggagcaaggg cgacgagggc cttgcccgct cccggctcag ggccctcggc
     5041 tacaaaagcg ccgagatcgc cgagctcttc ggcgaggggg atggggcacc ctaggcagcc
     5101 gaggcggccc gggcggccgt ggctcccagg caggcccggg cccccgagca ggccggcggc
     5161 cccccagccg gcaggcggcc tggggcaggg cttcccccgc gccgggggcg gacgatagta
     5221 ttacttgaca ttccttcgca tctgatccag aatcatgtct atatctgtga atccagagga
     5281 gtt
//
LOCUS       DASSQV010000410         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_346353, whole genome shotgun sequence.
ACCESSION   DASSQV010000410 DASSQV010000000
VERSION     DASSQV010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346353"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="VFL04_07130"
     CDS             <1..1342
                     /locus_tag="VFL04_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839518.1"
                     /translation="TDRRVKVVIPSMGDLFAAGTAAVLRAMGWNAEALEVCDREALDL
                     ARAVTTSKECLPIINILGELLKYLKHRRDPDERLVVVMVGAGGCCRVGQYKILIDALV
                     EKRWLENVAQLILTNDDGYAGLGLGFRLAMLKSLYLFDVLDDVRSAIKALAVDRQAGM
                     ELFGREVERLLAAIDGSSRVPLFTQMRRSAAALASIGLRTPIEQAKYVAVVGEIFVRR
                     DHFSLMGIPEMLAKRGFVMLDAPVSEWVRYTDFLRDIGMYTAKLGFLGRVEMLLSRLV
                     QDHYERRIKRLFVRSRLYEYEKIDIRAYMAHSTHFFPLELTGEPGLSSGSALKHLVDK
                     YCGVISVGPFGCMNSRMTEAVAAQEMTVAGKELAAANAGESLDLSELRAEVDVLPFLS
                     VECDGNPFSQIIQARLETFMLQADRLAGRLGRGTELEAARPARRGTVLGPARRG"
     gene            complement(1730..2116)
                     /locus_tag="VFL04_07135"
     CDS             complement(1730..2116)
                     /locus_tag="VFL04_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7839519.1"
                     /translation="MGRIIHFEIHAADFKRATVFYGELFGWKFESWGDGSYSVITTGP
                     DTSKGINGGMTKRTGPAPTAGQAVDGFVNIIEVPSVDEYTKKILAAGGSLSHGKETVP
                     SMGYSAYFRDPEGNLFGIFQIDAAAK"
     gene            complement(2200..>2770)
                     /locus_tag="VFL04_07140"
     CDS             complement(2200..>2770)
                     /locus_tag="VFL04_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839520.1"
                     /translation="IFERRLYELADAELEPDRILDLARGCEREILGLEVSPRPLLAIP
                     HLLGAESACSFQGYLLARMAVSQTRSQFLAAYGHLTDNPRIGRDLARAYWGPGNSLSH
                     DETVRGLTGKPIDPRPLAEDCNRDSEELWTAARASMEEALGRPRASTPEALSLEAEIH
                     IVHGAEEICDSRASLRELCDRFARWIAAR"
BASE COUNT          436 a          946 c          944 g          444 t
ORIGIN      
        1 caccgacagg cgcgtgaagg tcgtgatccc ctcgatgggc gacctcttcg ccgccggcac
       61 ggcggcggtc ctccgcgcga tgggctggaa cgccgaagcc ctcgaggtct gcgaccgcga
      121 ggccctcgac ctcgcccgcg cggtgacgac gagcaaggag tgcctgccga tcatcaacat
      181 cctcggggag ctgctcaagt acctcaagca ccggagggac ccggacgagc gcctcgtcgt
      241 cgtcatggtc ggggcggggg gctgctgccg cgtgggccag tacaagatcc tcatcgacgc
      301 cctcgtcgag aagcgatggc tcgagaacgt ggcccagctg atcctgacga acgacgacgg
      361 ctacgccggc ctcggcctcg gcttccgcct cgcaatgctc aagtccctct acctcttcga
      421 cgtgctcgac gacgtgcgct cggccatcaa ggccctggcg gtggacaggc aggcggggat
      481 ggagctcttc ggccgcgagg tggagcgcct cctcgccgcg atcgacggca gctcccgggt
      541 gcccctcttc acccagatgc gccgcagcgc cgccgccctc gcctccatcg ggctccggac
      601 gccgatcgaa caggcgaagt acgtcgccgt cgtcggcgag atcttcgtgc gcagggacca
      661 cttctccctc atgggcatcc ccgagatgct cgcgaagcgg ggcttcgtca tgctcgacgc
      721 tcccgtctcc gagtgggtgc gctacaccga tttcctgagg gacatcggca tgtacacggc
      781 caagctcggc ttcctcggca gggtggagat gctgctctcg cgcctcgtcc aggaccacta
      841 cgagcgcagg atcaagcgcc tcttcgtccg ctcccggctc tacgagtacg agaagatcga
      901 catcagggcc tacatggccc actcgaccca cttcttcccc ctcgagctca ccggggagcc
      961 ggggctctcc tcgggctcgg ccctgaagca ccttgtggac aagtactgcg gggtcatctc
     1021 ggtggggccc ttcggctgca tgaactcccg catgaccgaa gccgtggccg cccaggagat
     1081 gaccgtcgcc ggcaaggagc tggccgcggc caacgcggga gagagcctcg acctctcgga
     1141 gctccgggcc gaggtcgacg tcctcccctt cctctcggtc gagtgcgacg ggaacccctt
     1201 ctcccagatc atccaggcgc ggctcgagac cttcatgctc caggccgaca ggctcgcggg
     1261 gaggctcggg agggggacag agctcgaagc ggcgcggccg gcgcggcgag gtacagtgct
     1321 cggaccggcg cggcgagggt agggctcgat ggggtggcgg gaagaagcgg tgcacggctg
     1381 acgccggcct ggaccccgcg ccgtcgccgg aggcctaaga cgagaggttt ggcggcgccc
     1441 cgggagggac agtgctcgag tgcgccgtgc tcgagttccc cccttcgcgg tggggggccc
     1501 atcaggctcg ccgccgagtc ttgaggggat cagcggcgct ttctacatgg accgcatgga
     1561 gacaccctgc gaattccggg aggccctcga ggatcggctc tgctcccggc tgcgggaggc
     1621 cacgggctcc tgatccgcaa ggctgcagct ctgtccccgg ccgagccgga ccccggcagc
     1681 gccgtcagtc cggggaggga cagagctcgc agcctcgaca aactcgccct tacttcgcgg
     1741 ccgcgtcgat ctggaagatc ccgaagaggt tcccctcggg atccctgaaa taggcggaat
     1801 agcccatgga gggaacggtc tccttgccat gggagaggct ccctcccgcg gcgaggatct
     1861 tcttcgtgta ctcgtcgacg ctgggcacct cgatgatgtt gacgaagccg tccacggcct
     1921 ggccagccgt cggggccggc cccgtgcgct ttgtcatccc tccgttgatc cccttgctcg
     1981 tatcaggccc cgtggtgatc accgagtagg agccatcccc ccaggactcg aacttccagc
     2041 caaaaagctc cccgtaaaag accgtcgcgc gcttgaaatc cgcggcgtgg atctcgaaat
     2101 ggatgatgcg gcccatgtga acccccttaa gcaaggacga tatggcaaaa atagccacgc
     2161 ccctcctgtt cacccaagga cttctcgtcc ttcccgcggt tatcttgccg cgatccaccg
     2221 ggcgaagcgg tcgcacagtt cccgcagcga ggcgcggctg tcgcagatct cctcagcgcc
     2281 gtggacgatg tggatctccg cctcgaggga cagagcttcg ggggtcgagg cccgcggccg
     2341 ccccagcgcc tcctccatcg aggcacgggc ggctgtccag agctcctctg aatcccggtt
     2401 gcagtcctcg gcgagggggc gggggtcgat gggctttccg gtcaggcctc ggaccgtctc
     2461 gtcgtggctc aggctgttcc ccgggcccca ataggcgcgg gcgaggtccc ggccgatccg
     2521 ggggttgtcc gtcagatggc cgtacgcggc gaggaactgg gacctggtct gcgagacggc
     2581 catgcgggcg aggaggtagc cttggaagga acaggctgat tcggcgccga ggaggtgggg
     2641 gatcgccagg aggggccgcg gcgagacctc gaggccgagg atctcgcgct cgcagccgcg
     2701 ggcgagatcg aggatccggt ccggctcgag ctcggcgtcg gccagctcgt acaggcggcg
     2761 ctcgaagatc
//
LOCUS       DASSQV010000411         4820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_346591, whole genome shotgun sequence.
ACCESSION   DASSQV010000411 DASSQV010000000
VERSION     DASSQV010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346591"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..2267
                     /locus_tag="VFL04_07145"
     CDS             327..2267
                     /locus_tag="VFL04_07145"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7839521.1"
                     /translation="MGEKKKILILGGGYGGVWAGKILEKQFRKREDVSITLVDKRPFH
                     TLMTELHEVAGWRTEPESVQVSFKKIFGAKNIETVVDTIQRIDFEAKRAFSERSEYPF
                     DYIVIGAGAEPEYFGIPGVKENSFSLWSFDDAMRIRNHLEHIYAMAAREPDPARRRRM
                     LTFTVAGAGFTGSEMVGELLEYRDTMCRKHFLDPKEVRIIMIEALPAILPILETPLRE
                     KAEKYLVKHGCEIMLGKAIVGAEPGKVLLKDGGFVETDTFIWTCGVKGSSFSGSLPLP
                     MGKRGRIETDAEMKSPKYPFVYVVGDNAWYIHEEMPLAQIVESCHFSAEAAAKNIIAD
                     INGGERHKFKATYHGFMISLGGRYAVSNAGGMKTSGFMAIAMKHMINVYYLFCIAGAN
                     QAWEYLKHEILDMKENRSLIGGFASYKIRGYWPLLLRVWLGFMWVVEATNKITEGWLD
                     FSSGLSKTGWMFSQGVVQAGQKLAEATSAATAAGDAAGAVASTAAEATSAASATATAP
                     AAAVLGPWFDTTKNILDPSSGLVTWFRHTFMDGIFAHLPYSGFQIMIVLTELFIGLAL
                     LGGFFTWFAAVASIAMCLIFTLSGMFAWNQLWFLFAAILCMGGVGRAFGLDYWSVPFF
                     KKWWNGTKIARRTHLYGDDPTK"
     gene            2393..3379
                     /locus_tag="VFL04_07150"
     CDS             2393..3379
                     /locus_tag="VFL04_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET7839522.1"
                     /translation="MTDFWNDSPSLGSRIESVVSLMDETIASTDFPLAEALRGLVGSN
                     GKMLRPALLLIGARFGKGADDRRITALAASIELLHVATLIHDDILDEAELRRGVPTIH
                     TQFGTKEAVLAGDWLLSRSFRLASESAAPENAQALGRLIGAICSAEIGQDLGKYRYDC
                     SVRRYLRTIAGKTAALFSLALHAGATEAKVAPRLVVQRLRRAGYGIGMAFQVIDDILD
                     FESSEGVMGKPVGKDLREGLCTLPLIYALQADRGSLEGLLARSRKEKGQLDQASIDEI
                     VDLTARLGGLDRARETAHRFTARTLAEINGLPNVPARAELAALASKLLERTY"
     gene            complement(3425..4558)
                     /gene="dnaJ"
                     /locus_tag="VFL04_07155"
     CDS             complement(3425..4558)
                     /gene="dnaJ"
                     /locus_tag="VFL04_07155"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET7839523.1"
                     /translation="MAKRDYYEVLGVAKDATKDDIKKAYRRLAIQNHPDKNPGDKAAE
                     ERFKEATEAYEVLGDEQRRQAYDQFGFAGVEGMGGAGSAQDFSSVFKDFEDIFGGFGD
                     FSNIFDSFFGAQGGRGRGARERSRGANLRYDLELPFEQAVFGAAVEIAYSKNDSCKVC
                     AGSGSSDGTGKRVCPTCQGSGQVRRSSGFFSIAQTCPSCRGEGSIIEKPCRECGGSGL
                     AKKKQKIKVTIPPGVEDGKRVVIPAQGDTGPNGGVPGDLYVFIHVRPHDYFERDGADL
                     YCALPVSLTMAAIGGEITFSTIEGKKIKVAVPAGSQHGKMLRIREEGIPSSGGRRGDL
                     YLKLLVQVPAKLSRKGKELLEQLREVEGEDSEPKPVKLADLKN"
BASE COUNT          863 a         1623 c         1492 g          842 t
ORIGIN      
        1 tgtcggcggg gcggcttcgg cgccccggct cgtattggca aggccggctc cggccacagg
       61 tcgactcggg tctcggcaga aaagcccgct gaacagacat gatacatcgg agcgggcaaa
      121 aagtgaagtg aggctcgaat ccgagccggg cgcacgcaga caaggcccgg agctgcgcca
      181 agccgcgctt tctcttgata atgcgtatgg cccattcttg actttcaggg tacaattcga
      241 gcattatcgg tcggtttcac tgccagcatc aaatcgactg cacccaaaag tccaactcct
      301 acatgcacta caaggagcga tccgcgatgg gcgagaagaa gaaaatcttg atcctcggag
      361 gcggctacgg cggcgtctgg gctggaaaga ttctggaaaa acagttcaga aagcgtgagg
      421 atgtctcgat caccctcgtc gacaagaggc ccttccacac cctcatgact gagctgcacg
      481 aggtggccgg ctggagaacg gagccggaat ccgtccaggt cagcttcaag aagatcttcg
      541 gcgccaagaa catcgagacc gtggtcgaca cgatccagag gatcgacttc gaggccaagc
      601 gcgcgttctc cgagcggagc gaatatccct tcgactacat agtcatcggc gccggcgccg
      661 agcccgagta tttcggcatc cccggcgtca aggagaattc cttctccctc tggtccttcg
      721 atgatgcgat gaggatccgc aaccaccttg agcacatcta cgccatggcg gcccgcgagc
      781 ccgatcccgc gcgccgcagg cgcatgctca ccttcacggt ggcaggggcc ggcttcaccg
      841 gcagcgagat ggtcggcgag ctgctcgagt accgcgacac catgtgccgc aagcacttcc
      901 ttgatccgaa ggaagtgagg atcatcatga tcgaggccct gcccgcgatc ctcccgatcc
      961 tcgagacccc cttgcgcgag aaggccgaga aataccttgt gaagcacggc tgcgagatca
     1021 tgctcggcaa ggcgatcgtc ggcgccgagc cggggaaggt cctcctcaag gacggcggct
     1081 tcgtcgagac tgacaccttc atctggacct gcggcgtcaa gggctcctcc ttctcgggat
     1141 ccctgcctct ccccatgggc aaacgcggcc ggatcgagac cgacgccgag atgaagtcgc
     1201 ccaagtaccc cttcgtctac gtcgtcggcg acaatgcctg gtacatccac gaggaaatgc
     1261 ccctcgccca gatcgtcgag agctgccact tctccgccga ggccgccgcg aagaacatca
     1321 tcgcggacat caacggcggg gagcgccaca agttcaaggc cacctaccac ggcttcatga
     1381 tctcccttgg cggccgctac gccgtctcga acgccggagg gatgaagaca agcggcttca
     1441 tggcaattgc gatgaagcac atgatcaatg tctactacct cttctgcata gcgggcgcga
     1501 accaggcctg ggaatacctg aagcacgaga tcctcgacat gaaggagaac cgctccctca
     1561 tcggcggttt cgcgtcttac aagatccgcg gctactggcc cctcctcctc agggtctggc
     1621 tcggcttcat gtgggtcgtc gaggccacga acaagatcac cgagggctgg ctggacttct
     1681 cgagcggtct cagcaagacc ggctggatgt tctcccaggg ggtcgtccag gccggccaga
     1741 agctcgccga ggccacgagc gcggcaaccg cggccggcga cgccgccggg gcggtcgcct
     1801 cgaccgcggc cgaggccacg agcgcggcca gcgccacggc cacggccccg gccgccgccg
     1861 tcctgggccc ctggttcgac acgacgaaga acatcctcga tccgtcctcc ggcctcgtca
     1921 cctggttcag gcacaccttc atggacggga tcttcgccca tctgccctat tcgggcttcc
     1981 agatcatgat cgtgctcacc gagctcttca tcggcctcgc cctcctcggc ggcttcttca
     2041 cctggttcgc ggcggtggcg agcatagcga tgtgcctcat attcacactc tctggcatgt
     2101 tcgcgtggaa ccagctctgg ttcctcttcg ccgcgattct ctgcatgggc ggcgtgggaa
     2161 gggccttcgg cctggactac tggtcggtgc ccttcttcaa gaaatggtgg aacggcacga
     2221 agatcgcccg gcggactcac ctctacgggg acgacccgac gaagtgaggc cccgcccgga
     2281 ggcctctgat ccgtgagacc gcagtcccgc acaccgtccc ttatggggcg gtgtgcgggg
     2341 acaagggcca attctccagc atggcaatcc ccggaattgg ccaaaggacc gcatgacgga
     2401 tttctggaac gactcgccga gcctcgggtc gcggatcgag agcgtcgtct ccctcatgga
     2461 cgagacgatc gccagcaccg acttccctct ggccgaggcc ctgcgcgggc tggtcggctc
     2521 aaacggcaag atgctgcgcc ccgcgcttct cctcatcggc gcccgcttcg gcaagggcgc
     2581 ggacgacaga cgcatcaccg ccctcgcggc atccatcgaa ctcctccacg tggcgaccct
     2641 catccacgac gacatcctcg atgaggccga gctcagacgc ggcgttccta cgatccacac
     2701 ccagttcggc acaaaggagg cggtgctcgc gggcgactgg ctcctgtccc ggagcttcag
     2761 gctcgcctca gagagcgcgg cccccgagaa tgcccaggcc cttggccgcc tgatcggcgc
     2821 catctgctcg gccgagatcg gccaggacct cgggaaatac cgatacgact gcagcgtgag
     2881 gcgctacctc aggaccatcg ccggaaagac ggccgccctc ttctcgctcg ccctccatgc
     2941 gggggcgacg gaggcgaagg tcgccccccg gctggtggtc cagcggctca ggagggcggg
     3001 ctacgggatc ggcatggcct tccaggtcat cgatgacata ctggacttcg agagcagcga
     3061 gggggtcatg ggcaagccgg tgggcaagga cctcagggag ggcctctgca cccttcccct
     3121 catctacgcc ctgcaggcgg atcgtggctc cctcgagggc ctgttggccc gttcgcggaa
     3181 ggaaaagggc cagcttgacc aggccagcat cgacgagatc gtggatctga cggcgagact
     3241 cggcggcctg gacagggccc gggagacggc gcaccgcttc accgcgagaa cccttgccga
     3301 gatcaacggc ttgcccaacg tgccggcccg cgccgaactt gcggccctcg cgtccaagct
     3361 tctcgagcgc acctactgag ccccgacgca tgaggtcgga ggcggcggcg agaccgctgg
     3421 cctgtcagtt cttgaggtcg gcgagcttga cgggcttggg ctcggaatcc tctccctcca
     3481 cctcgcgaag ctgctcgagc agttccttgc ccttgcggct cagcttggcg gggacctgga
     3541 ccaggagctt taggtaaagg tcgccgcggc gcccgcctga agagggtatg ccctcctcgc
     3601 ggatcctcag catcttgccg tgctgggagc cagccgggac ggcgaccttt atcttcttgc
     3661 cctcgatggt ggagaaggtg atctcgcccc cgatggcggc catcgtcaga ctgacgggca
     3721 gggcgcagta gaggtcggct ccgtcccgct cgaagtagtc gtgcggacgt acgtggatga
     3781 agacatagag gtcgccgggg actccgccgt tcggaccggt gtcgccctgg gccgggatga
     3841 cgacacgttt gccgtcctcc accccgggcg ggatcgtgac cttgatcttt tgcttcttct
     3901 tggcaaggcc cgagccaccg cactctctgc agggcttttc gatgatggag ccctcgccgc
     3961 ggcaggacgg gcaggtctga gcgatcgaga agaagcccga gctccggcgc acctggcccg
     4021 aaccctggca ggtcgggcac acgcgtttgc ctgtcccgtc tgaggagccc gagcccgcgc
     4081 agaccttgca ggagtcgttc ttggaatagg ctatttcgac ggccgccccg aacacggcct
     4141 gctcgaaggg gagctcgagg tcgtagcgga ggttggcgcc acggctgcgc tcgcgcgccc
     4201 cgcgtccacg ccccccctgg gcgccgaaga aggaatcgaa gatattcgag aaatccccga
     4261 agcctccgaa gatgtcctcg aaatccttga acaccgagga gaagtcctga gccgagcccg
     4321 ccccgcccat accctcgacc ccggcgaagc cgaactggtc gtaggcctga cggcgctgtt
     4381 cgtcgccgag cacctcgtag gcctccgtgg cctccttgaa ccgctcctcg gcggctttgt
     4441 cgcccgggtt cttgtcggga tggttctgga tcgcgagtcg cctgtacgcc ttcttgatgt
     4501 cgtctttggt cgcgtccttt gcgactccga gaacctcgta gtaatcgcgc ttggccacag
     4561 ggcttcctta agaaaagatg gccacggggg caccgagacg ccggagcctg agctcccgct
     4621 gtcccatgcc ccggcggcaa tatctaaagc ttctaccgat ccctatttct tgtcctcgtc
     4681 atcgacgacg cggtagtcta cgtcctccgc cgagcctgaa gccgagggac ctgcccccgg
     4741 cccggcggac tctccaccgc cctgctcggc tccaccggga ccagcggcgc caccctgggc
     4801 ccctgcggtc ttgtagatct
//
LOCUS       DASSQV010000412         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2089239, whole genome shotgun sequence.
ACCESSION   DASSQV010000412 DASSQV010000000
VERSION     DASSQV010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089239"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="VFL04_07160"
     CDS             <1..1855
                     /locus_tag="VFL04_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET7839524.1"
                     /translation="GDGDAGEEPIRLVLGAAAPPGPRHTAAEPGAEPRGPRQERPAAE
                     AEAPEPRPTRADADHGASAIRVDGIGEIEIITVKPESHGGNDAEDARKAAALARVLAR
                     RKDQPYAVPVDGILQAYPDGEYWIVDEKTKRSAEVLRDTLAEFNIEAEVTGIRKGPVI
                     TMFEILPAPGVKLSKITNLSDNIALRLAASSVRIVAPIPGKHAVGIEVPNERRSIVSL
                     REIVENTAFTGAKMEVPIILGKDIAGEVQFIDLVQTPHLLIAGATGSGKSVCVNSIIL
                     SILYRRSPREVKLLLIDPKIVELKLYNDIPHLLTPVITEPKRAFQALQYCICEMERRY
                     SMLDRVGVRDVKSYNRKVKEKNLAVERLPYIVVIIDEFADLMATTGKELESTLARLAA
                     MSRAVGIHLVLATQRPSIDVITGLIKANIPSRIAFMVASKFDSRIIIDMVGAEKLLGR
                     GDMLVASAWDPFPIRMQGAFVSEEEVEKAVDYIRTLGEPEYIDDEIFIDEDEEDEPSL
                     FGEEDDDPLFEKALEIVLQQGKASASYIQRRLKIGYNRASRLVELMEEKGIVGPAKGS
                     KPRDLVRNPEVLGKPMSPPAARQPSLMPDLETLPGQRVTGRDEPADEDPED"
     gene            1876..3060
                     /locus_tag="VFL04_07165"
     CDS             1876..3060
                     /locus_tag="VFL04_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET7839525.1"
                     /translation="MRRIGILGGGQLGRMAMEEARKYLAEIEVLTPDYPSPGAELADF
                     AHIGELDDYEGLLEFGAGVEALSWEIESVGLEALYELERRGKAVLPGAAVLEAIQDKA
                     SQKAILEAKGIPTARWLHLESAPGGGPDSRGLEAAAEALGGFPIVQKARRGGSDGRGV
                     QVLGSAGSQALKMPSIAEERIDFAKELAVVVARGPEGSTAVYPCVEMVFDERANLCDS
                     VLAPAREGEAIRSRAERLALDCVAALDGWARERTPGGSGAVGVFGVEMFLTRDGEVLV
                     NEIAPRPHNSGHFSIEACPSSQFDQYIRILLGLPLGSAELLRPAMMVNIIGEPGQTGE
                     PVYLGLDAALAIPGVAVHLYGKRELRPFRKMGHLTAIGRSTKEAMDRAQAARAMIRVV
                     AR"
BASE COUNT          554 a         1093 c         1032 g          426 t
ORIGIN      
        1 cggggacggg gacgcgggtg aagagccgat ccgcctcgtc ctcggcgcgg cggccccccc
       61 ggggccccgc catacggccg ccgagcccgg ggcggagccc cgaggtcctc gtcaagagag
      121 gccggcggcc gaggccgagg cgcccgagcc gaggcccacc agggccgatg ccgaccatgg
      181 cgcctcggcg atcagggtcg acgggatagg cgagatcgag atcatcacgg tcaaacccga
      241 gagccacggc ggcaacgatg ccgaggatgc ccggaaggcc gcggccctgg cccgcgtcct
      301 cgcgcgacgc aaggaccagc cctacgccgt cccggtcgac ggcatcctcc aggcctatcc
      361 cgacggggag tactggatcg tcgacgagaa gacgaagcgt tcggccgagg tcctcaggga
      421 caccctcgcc gagttcaaca tcgaggccga ggtcacgggg atccgcaagg ggccggtgat
      481 cacgatgttc gagatcctgc cggccccggg ggtcaagctc tccaagatca cgaacctctc
      541 cgacaacatc gccctccgcc tcgccgcctc gagcgtccgc atcgtggcgc ccatcccggg
      601 caagcacgcc gtgggcatcg aggtgccgaa tgagaggcgc tccatcgtct ccctccgcga
      661 gatcgtcgag aacacggcct tcaccggggc gaagatggag gtgccgatca tcctcggcaa
      721 ggacatcgcc ggagaggtgc agttcatcga cctcgtccaa accccccacc tcctcatcgc
      781 aggcgccacg ggctcgggca agtcggtctg cgtgaactcg atcatcctct cgatcctcta
      841 ccgacggtcg ccgagggagg tgaagctcct cctcatcgac cccaagatcg tcgagctcaa
      901 gctctacaac gacatccccc acctcctgac cccggtcatc accgagccca aacgcgcctt
      961 ccaggccctc cagtactgca tctgcgagat ggagcggcgc tacagcatgc tcgacagggt
     1021 cggggtccgc gacgtgaaga gctacaaccg caaggtcaag gagaagaacc tcgctgtgga
     1081 gcgcctcccc tacatcgtcg tcatcatcga cgaattcgcg gacctcatgg cgacgacggg
     1141 caaggagctc gagtctaccc tggcccgcct cgcggccatg tcgagggccg tcggcatcca
     1201 cctcgtcctc gcgacccagc gcccctcgat cgacgtgatc accggcctca tcaaggcgaa
     1261 catcccgagc cggatcgcct tcatggtggc gagcaagttc gactcccgca tcatcatcga
     1321 catggtcggg gccgagaagc tcctcggccg gggcgacatg ctcgtcgcga gcgcctggga
     1381 ccccttcccg atccgcatgc agggtgcctt cgtctccgag gaggaggtcg agaaggccgt
     1441 cgactacatc aggaccctgg gcgagcccga gtacatcgac gacgagatct tcatcgacga
     1501 ggacgaggag gacgagccct cgctcttcgg cgaggaggac gacgatcccc tcttcgagaa
     1561 ggccctcgag atcgtcctcc agcagggcaa ggcgagcgcc tcctacatcc agaggaggct
     1621 caagatcggc tataaccggg cctcccgcct ggtcgagctc atggaggaga agggcatagt
     1681 cgggccggcc aagggctcca agccccgcga cctcgtccgc aaccccgagg tcctcggcaa
     1741 gccgatgagc ccgccggcgg cccgccagcc gagcctcatg cccgatctgg agaccctccc
     1801 cggtcagagg gtgacggggc gtgacgagcc tgctgacgag gatccggaag actgaatcgt
     1861 ggggcggagg ccagggtgag aaggatcgga atactgggcg gcggccaact cggccgcatg
     1921 gccatggagg aggcgaggaa gtacctcgcc gagatcgagg tgctcacgcc ggactacccc
     1981 tcccccgggg ccgagctggc cgacttcgcc cacatcgggg agctggacga ctacgagggc
     2041 ctcctcgagt tcggcgcggg ggtcgaggcc ctctcatggg agatcgagag cgtcggcctc
     2101 gaggccctct acgagctcga gcgccgcggc aaggccgtcc ttcccggcgc ggccgtcctc
     2161 gaggcgatcc aggacaaggc gagccagaag gccatcctgg aagcgaaggg catccccacg
     2221 gcccgctggc tccacctgga atcggctccc ggaggggggc ccgattcccg cggcctcgag
     2281 gcggcggccg aggcactcgg gggcttcccc atcgtgcaga aggcaagacg cggcggctcc
     2341 gacgggcgcg gagtccaggt cctcggctcg gccgggagcc aggccctgaa gatgccctcg
     2401 atcgccgagg agcgcatcga cttcgccaag gagctcgccg tcgtggtcgc ccgcggcccc
     2461 gagggctcca cggcggtgta tccctgcgtc gagatggtct tcgacgagcg cgccaacctc
     2521 tgcgactccg tgctcgcccc cgcgcgcgag ggcgaggcca tccgctcccg ggccgagcgc
     2581 ctcgccctgg actgcgtcgc ggccctggat ggctgggcca gggagaggac ccccggaggc
     2641 tcgggggccg tgggagtctt cggcgtcgag atgttcctga cccgagatgg tgaagtcctc
     2701 gtcaacgaga tcgccccacg cccgcacaac tctgggcatt tctcgatcga ggcctgcccc
     2761 tcgagccagt tcgaccagta catcaggatc ctccttggcc tgcccctcgg gagcgccgag
     2821 ctcctgcggc cggccatgat ggtgaacatc atcggggagc cgggacagac aggcgagccc
     2881 gtctacctcg gtctggacgc ggcgctcgcc atacccgggg tcgccgtcca cctctatggc
     2941 aaacgggagc tgaggccctt ccgcaagatg ggccacctca ccgccatagg caggagcacc
     3001 aaagaggcga tggacagggc ccaggccgcc agggcgatga tcagggtggt cgcgagatga
     3061 gccgccgcct ataatgccgg agcatggaac cggtaccgac cctta
//
LOCUS       DASSQV010000413         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_223949, whole genome shotgun sequence.
ACCESSION   DASSQV010000413 DASSQV010000000
VERSION     DASSQV010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223949"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..858
                     /locus_tag="VFL04_07170"
     CDS             112..858
                     /locus_tag="VFL04_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET7839526.1"
                     /translation="MPEQELSRFAVILCRAEEPGNVGSVCRAMKTMGLTSLVLAGCPE
                     YDEARVRTMAVHAADVFEAAQRFPSLEAALAGFALSGGFTRRRGERRKSFSLSAADFA
                     QRVLEGPAARARGRVALVFGNERTGLTEEELGLCSLAVHIPSSSAFPSLNLAQAVQIA
                     CYELRRNAMGEIDGSAEPVTRQEVDAAVSRIAEDLRGLGFFRQTGGSRLVAFLRDTVE
                     RSAYTPSELRYYENLFHKMAGLARRGGPVA"
     gene            complement(855..1964)
                     /locus_tag="VFL04_07175"
     CDS             complement(855..1964)
                     /locus_tag="VFL04_07175"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7839527.1"
                     /translation="METLLFLADDPEEARALVAELAGHYTVIPCPDEVAAIEAARLRK
                     PAALVVDSKALGRRGDGRVGTARLGRIRRVAGEAPIIALLQPGEDRGAAQLREAKALI
                     QRPCGLGHIREAVDAAVGVARANPASPFHGSSAAILATAERLRLYAGSDYPVLILGES
                     GTGKEIAARAIHELSPRSHAPFVDRNCSALPEQLAESELFGTERGAFTDAVARPGAFE
                     LARGGVLFLDEIGDAGPSLQAKLLRVLESGQCWRLGARSPIQVDARIVSATAADLRAA
                     AAAGSFRSDLLYRIETLVLELPPLRSRREDIPELASRIALEASRGRVSIAASALARLA
                     AHDWPGNVRELRNVVHRALVLAGDSPLITERELQF"
BASE COUNT          288 a          766 c          763 g          302 t
ORIGIN      
        1 ggggccggcg ggtctccccg ccggccccga tcttgttgct ctggcatgct gcgggtacta
       61 tagcccccgg gattcgccgc ggcaaggcgg gtcttccgca aaggtgggcc catgcctgaa
      121 caggagctct cgcgctttgc cgtcatcctc tgccgcgccg aagagccggg gaacgtcggc
      181 tcggtctgcc gggccatgaa gaccatgggc ctcaccagcc tcgtgctggc cggctgtccc
      241 gagtacgacg aggcgcgcgt aaggacgatg gccgtccacg ccgccgacgt cttcgaggcc
      301 gcccagcgct ttccgagcct cgaagccgcc ctcgccggct tcgccctctc gggcggcttc
      361 acgaggcggc ggggcgaacg gcgcaagtcc ttctcgctca gcgccgccga tttcgcccag
      421 cgggtcctcg aggggccggc tgccagggcc aggggtaggg tcgccctcgt cttcggcaac
      481 gagaggacgg ggctcacgga ggaggagctc ggcctctgca gcctcgccgt ccacataccc
      541 agctcctcgg ccttcccctc gctcaacctc gcgcaggccg tccagatcgc gtgctacgag
      601 ctcaggcgga acgcgatggg cgaaatagac ggctcggccg agcccgtgac gcggcaggag
      661 gtcgacgccg cggtgtcccg gatcgccgag gacctccgcg gcctgggctt cttccgccag
      721 acaggcggct cgcggcttgt cgccttcctg cgggacacgg tcgagcgctc ggcctacacg
      781 ccctcggagc tccgctacta cgagaacctc ttccacaaga tggccggcct ggcccgcagg
      841 ggcggccccg tcgcctagaa ctgcagctcc ctctcggtga tcaggggcga gtcgcccgca
      901 agcaccaggg cgcgatgcac gacattgcgc agctcccgca cattccccgg ccagtcgtgg
      961 gcggcgagcc tggccagggc cgaggcggcg atggagaccc ggccccgcga ggcctcgagc
     1021 gcgatgcgcg acgccagctc cgggatgtcc tcccgccgcg acctcagggg cggcagctcg
     1081 aggacgaggg tctcgatccg gtacagcagg tcagagcgga agctccccgc cgccgcggcc
     1141 gcccttaagt cggccgccgt ggccgagacg atcctcgcgt cgacctggat ggggcttctg
     1201 gcgccgaggc gccagcactg ccccgactcg aggacgcgga ggagcttggc ctggagcgag
     1261 gggccggcgt ccccgatctc gtccaggaag agcacgccgc ccctcgccag ctcgaaggcc
     1321 cccggccggg cgacggcgtc ggtgaaggcg ccgcgctccg tgccgaagag ctcgctctcg
     1381 gcgagctgct cgggcagggc cgagcagttg cggtcgacga agggggcatg gctccgcggc
     1441 gagagctcat ggatggcccg cgcggctatc tcctttccgg tgccgctctc gcccaggatg
     1501 aggacgggat agtccgagcc cgcgtagagc ctgagccttt cggccgtggc gaggatcgcc
     1561 gcgctgctgc cgtggaacgg cgatgcgggg ttcgcgcggg ctaccccgac cgcggcgtcg
     1621 accgcctcgc ggatgtggcc gaggccgcag ggcctctgga tgagggcttt cgcctcgcgg
     1681 agctgggccg cccccctgtc ctccccaggc tggagcaggg cgatgatcgg agcctcgccc
     1741 gctaccctcc tgatccggcc aagcctggct gtccccaccc gtccatcgcc gcggcggccg
     1801 agggccttcg agtcgacgac cagggcggcc ggcttcctga gccgggccgc ctcgatggcc
     1861 gcgacctcat cggggcaggg gatgaccgtg tagtggcccg cgagctccgc gacgagggct
     1921 ctggcctcct cggggtcatc ggcgaggaaa aggagggttt ccatggcctt tgcatcccga
     1981 tcctaagcca accggggcgg gacagacagc agcgccgggc gcggctcggc ccctcggcgc
     2041 cgggcgccgc ctaggcatga gcctaatggg ctctacgccc gtgtaggccc tagcggtggc
     2101 tgagccgggt gaagaggag
//
LOCUS       DASSQV010000414         8347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_224026, whole genome shotgun sequence.
ACCESSION   DASSQV010000414 DASSQV010000000
VERSION     DASSQV010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224026"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFL04_07180"
     CDS             complement(<1..313)
                     /locus_tag="VFL04_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET7839528.1"
                     /translation="MNLIEAIRGGSTLELPLVYVRDAVVFPKAIVPLLAGTKFSVAAV
                     EEALRGEKRIVAALLKSIGDEKGAEIEVHAVATVARIVQQVRLDGSVRLLVEGEARVR
                     VR"
     gene            complement(310..1560)
                     /gene="clpX"
                     /locus_tag="VFL04_07185"
     CDS             complement(310..1560)
                     /gene="clpX"
                     /locus_tag="VFL04_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET7839529.1"
                     /translation="MAKQKGNSPTEDRTCSFCGKSAEFARRLIAGPGVFICDECVKVC
                     DQILSEDENEIAAGFTGEVPKPREVKEYLDQYVVGQEYVKRALSVAVYNHYKRIAHGR
                     DHASEVEIEKSNVLLIGPTGTGKTLIARTLAKKLKVPFAMADATTLTEAGYVGEDVEN
                     ILLKLIQNAGGNISAAERGIVYIDEIDKIARKGENISITRDVSGEGVQQALLKIIEGT
                     TANVPPQGGRKHPQQEYLRIDTTNILFICGGAFVGLDKIIENRVSQHALGFGADVKPR
                     GDKDIQALFQQLHPDDLIKFGMIPEFIGRLPIQVSLSDLSRDDLKRIITEPRNAVLRQ
                     YQESMRIDGVELHFDEGAVSAIADKALARKTGARGLRSIVEALMMDVMYDIPSIEGAK
                     RVVVTRETVEGSLAPAVEILQKSA"
     gene            complement(1538..2158)
                     /locus_tag="VFL04_07190"
     CDS             complement(1538..2158)
                     /locus_tag="VFL04_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET7839530.1"
                     /translation="MDERMHNLVPIVIEQTGIGERSYDIYSRLLKDRIVFIDGEINDL
                     TADLTVAQLLFLESQDPEKDISIYINSPGGSVTSGLAIYDTIQCIRPDVQTFCLGQAA
                     SMAALILACGAKGKRFALPSSRVLIHQPWGGVSGQASDISLQAREIVRLKKLTIDYFA
                     AAAGKDREVVAADMERDFFMSPEEAKAYGLVDAIFERRKHGQAEGK"
     gene            complement(2313..3671)
                     /gene="tig"
                     /locus_tag="VFL04_07195"
     CDS             complement(2313..3671)
                     /gene="tig"
                     /locus_tag="VFL04_07195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET7839531.1"
                     /translation="MIASKNVEKLEHSTVKLSVVVPKDEVQKEYSAMLADYSRNVRID
                     GFRKGHVPQAVLERKFGGELKLDAMGRVMEKAVEEAVTDIEEKPLAYSSPRLDGEPDF
                     SVDKDFSFAVIYDVFPTVEIADWKGMEIEIPEGEIAQEDEAQELADIRERNAIVVEKD
                     SSAAAAKGDVVTLNYSELGEDGQAVAGSERQDFTFEIGTGYNLYKFDDEIVGMKRDEE
                     KKVQKAFPADYEYKELAGRTVSIQVKLTKIKEKKLPELDDDFAQDVSEKYKTLADLKA
                     DIRKHLEKRLADKLRQLKEKAIVDGLLERAKADLPRSMVDAELGMRLENLKRQMQIDA
                     DDKLDRLLSFSGKTQLGLVEEWRPSAEKAITTRLVLDKLAEEGKYECSEADLEAEFKR
                     QAEENSISVDEVKAEYEKRGSMDYLRDRIKEDKLMEDILAAAKVKKGKKRAYVDLIKE
                     NE"
     gene            complement(3778..3849)
                     /locus_tag="VFL04_07200"
     tRNA            complement(3778..3849)
                     /locus_tag="VFL04_07200"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3815..3817),aa:Gly,seq:gcc)
     gene            complement(3881..3961)
                     /locus_tag="VFL04_07205"
     tRNA            complement(3881..3961)
                     /locus_tag="VFL04_07205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3926..3928),aa:Leu,seq:tag)
     gene            complement(4085..4157)
                     /locus_tag="VFL04_07210"
     tRNA            complement(4085..4157)
                     /locus_tag="VFL04_07210"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4122..4124),aa:Lys,seq:ctt)
     gene            complement(4176..4249)
                     /locus_tag="VFL04_07215"
     tRNA            complement(4176..4249)
                     /locus_tag="VFL04_07215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4213..4215),aa:Arg,seq:tcg)
     gene            complement(4301..4373)
                     /locus_tag="VFL04_07220"
     tRNA            complement(4301..4373)
                     /locus_tag="VFL04_07220"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4338..4340),aa:His,seq:gtg)
     gene            complement(4417..5532)
                     /gene="amrS"
                     /locus_tag="VFL04_07225"
     CDS             complement(4417..5532)
                     /gene="amrS"
                     /locus_tag="VFL04_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HET7839532.1"
                     /translation="MDEGPLGGARPAEFWKPLAGGDISCLLCPHRCRIGPGKKGICRV
                     RVNEGGRLVLPFYGAISGAGMDPVEKKPLYHFLPGTQVFSLGFVGCNLHCPFCQNWDI
                     SQSTESVAKRMEAEDVIKAARRSGSPSIAYTYSEPSVHYEFVLDCMRLARESGIRNIL
                     VTNGCLQAGPAAELLAMTDAANVDLKCWSAERYRKELGGDLDAVLGFIREASARCHLE
                     VTTLVVPDLSDGSEDVAAIAAFLADLDPDIPLHLSAYHPAWRHRAPATDPDALLELAL
                     VARRFLRYVYVGNLSGIESDTRCAGCGALLVRRRGYAVSVPGLGPAAIEVARGGPGRA
                     ARAAGAAAPSSSDPPKAPWGPRPVIEQNHSFFSPKAH"
     gene            complement(5573..5950)
                     /locus_tag="VFL04_07230"
     CDS             complement(5573..5950)
                     /locus_tag="VFL04_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839533.1"
                     /translation="MSITEYLSAEPFSEIIRYHQAESPQDAVAFTGTIRKHPYDEDKC
                     LLIGDAADREPAIFEFRIADVLSAVEMPAPVNELGTPRPLIKLWVRRGSFGIRYEPFE
                     VDEPLRFAAEPGHIRERFMGGAR"
     gene            complement(5922..7640)
                     /gene="lnt"
                     /locus_tag="VFL04_07235"
     CDS             complement(5922..7640)
                     /gene="lnt"
                     /locus_tag="VFL04_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET7839534.1"
                     /translation="MRTSPEDWHAPGQKPRHSSHPATLLALGLSSSFLLSLGLPNEVF
                     RLGMPLLGLVALIPLYVALRGCRSYGMAALVTGLFGACQHAMSSYWLYFFQDFAFWTI
                     GISTAAYAVVYSVLGLYLGLILRRSGAGRPLAFAAMWAGFEYLKSNGFLGYPWGLIPY
                     SLTGVLPLLQIADVTGVYGISALLAFANASLAELLAGKPSPMISRSEGSLLHHRTLSR
                     NPLTGRQMRGYLVAAALAILLTAGYGWARMGIRIPEIGSFRALLVQQNNDPWLVGEEA
                     GLSANVELAERALAEGGAKPDIVVFSETSLRRPYAEFRQYFSTEPAGHPLIPFIAKTG
                     VPLLTGAPVVTDWKTFTATNSVLLIGADPRLVASYAKIHPVPFAEAIPFWDLAWFRNF
                     VQKVVGLDSGWALGKDYTLFRLETRGGSFSFGTPICFEDAFPEVCRPFFLSGADLLIN
                     LTNDSWSKTVSAEIQHWAAARFRSIEFRRTLVRSTNGGLSCVVGPYGELLAAMPLFEA
                     SSRIVEVPVYRTAAPTFYESHGDLFAALLVLLSATWSIILMGREHDRGSVRRRRTHEH
                     NRIPLG"
     gene            7751..8230
                     /gene="coaD"
                     /locus_tag="VFL04_07240"
     CDS             7751..8230
                     /gene="coaD"
                     /locus_tag="VFL04_07240"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET7839535.1"
                     /translation="MVRAVFPGSFDPPTFGHLNIVERARSIFEELFVVVAVNRQKSGF
                     FSPEERRDLLLELLAPYPNVSVMLCDTLIVDFAKKHDCKVLVRGVRSVPDFSYEFELS
                     IMNKGLDPSIETIFMPTDPRFFVLRSSAIKELASFHGDLSAMVPPSVVKALKAKVGP"
BASE COUNT         1398 a         2631 c         2796 g         1522 t
ORIGIN      
        1 gcctaacgcg gaccctggcc tcgccctcga cgaggagccg gaccgagccg tccagcctga
       61 cctgctgcac gatcctcgcg acggtggcga ccgcatggac ctcgatctcg gcacccttct
      121 cgtcgccaat gctcttgagc agggccgcca cgatccgctt ctcgccgcgg agggcctcct
      181 ccacggcggc gaccgagaac ttggtgccgg ccaggagggg cacgatcgcc ttggggaaga
      241 ctaccgcgtc gcggacgtag acgaggggaa gctcgagggt gctgccccct cggattgcct
      301 cgatgaggtt cacgcgctct tttggaggat ctctacggcg ggcgcgaggc tgccctcgac
      361 ggtctcgcgc gtgacgacca cccgcttggc gccctcgatc gaggggatgt cgtacatcac
      421 gtccatcatg agggcctcga cgatcgaccg cagacctcgg gcgccggtct tccgagcgag
      481 ggccttgtcg gcgatcgccg agaccgctcc ctcgtcgaag tggagctcca ccccgtcgat
      541 gcgcatcgac tcctggtact ggcgcagcac cgcgttgcgc ggctccgtga tgatccgctt
      601 gaggtcgtcc ctcgagaggt cgctcagcga gacctggatc ggcagacggc caatgaactc
      661 ggggatcatg ccgaacttga tgaggtcgtc gggatggagc tgctggaaga gggcctggat
      721 gtccttgtcc cccctgggct tgacgtcggc gccgaagccg agggcgtgct gggagacgcg
      781 gttctcgatg atcttgtcga ggcccacgaa ggccccgccg cagatgaaga ggatgttggt
      841 cgtgtcgatc cgcaggtatt cctgctgggg gtgcttcctc ccgccctggg ggggcacgtt
      901 ggctgtcgtg ccctcgatga tcttgaggag ggcctgctgg accccctcgc ccgagacgtc
      961 ccgcgtgatg gagatgttct cgcccttgcg cgcgatcttg tcgatctcgt cgatgtagac
     1021 gatgccccgc tcggcggcgc tgatgttgcc gccggcgttc tggatgagct tgaggaggat
     1081 gttctcaacg tcctcgccaa cgtagcccgc ctcggtgagg gtggtcgcgt cagccatggc
     1141 gaaggggacc ttgagcttct tggcgagggt cctggcgatc agggtcttgc ccgtgcccgt
     1201 ggggccgatg aggaggacgt tcgacttctc gatctcgacc tcggaggcgt ggtcacggcc
     1261 gtgggcgatg cgcttgtagt ggttgtagac cgcgacggac agggcccgct tgacgtactc
     1321 ctggcccacg acgtactggt cgaggtactc cttgacctct cggggcttgg gcacctcgcc
     1381 cgtgaagccc gcggcgatct cgttctcgtc ctcggagagt atctggtcgc agaccttgac
     1441 gcattcgtcg cagatgaaga cgcctgggcc ggcgatgagc cgccgggcga actccgcgct
     1501 cttgccgcag aacgagcagg tgcggtcctc tgtggggcta tttcccttct gcttggccat
     1561 gcttcctccg ctcgaagatc gcgtcgacga ggccgtatgc cttggcctcc tcgggggaca
     1621 tgaagaagtc gcgctccatg tcagcggcca cgacctccct gtccttgccc gcggccgccg
     1681 cgaagtagtc gatggtgagc ttcttgagcc ggacgatctc gcgggcctgg aggctgatgt
     1741 cggaggcctg gcccgagacc ccgccccagg gctggtggat caggacccgg gaggagggca
     1801 gggcgaagcg cttgcccttg gccccgcagg cgaggatcag ggcggccatc gaggcggcct
     1861 ggccgaggca gaaggtctgg acgtcgggcc tgatgcattg gatcgtgtca tagatggcga
     1921 ggcccgaggt caccgacccg ccgggggagt tgatgtagat ggagatgtcc ttctcggggt
     1981 cctgggactc gaggaagagg agttgggcga ccgtgaggtc ggccgtgagg tcgttgatct
     2041 cgccgtcgat gaagacgatc ctgtccttga gcagcctgga atagatgtcg tacgaccgct
     2101 caccgatgcc cgtctgctcg atgacgatag gcaccaggtt atgcattctc tcgtccattg
     2161 ctgcacctct cgtgatggct atatcaagac tcggccggaa tcggcccggt ccgtagcgga
     2221 ccttgcgttt tcctcttcac ttcgcatggc cggaatgcga ggaagcgacc ccgagatgcc
     2281 gcgacatcat cgtgatcgct tccacatgaa aattactcat tctccttgat caagtccacg
     2341 taggcccgct tcttgccctt cttgaccttg gccgcggcca ggatgtcctc catgagcttg
     2401 tcttccttga tcctgtccct gaggtagtcc atcgagccgc gcttctcgta ctcggccttg
     2461 acctcgtcca ccgagatcga gttctcctcg gcctggcgct tgaactcggc ctcgaggtcg
     2521 gcctcggagc actcgtattt cccctcctcg gcgagcttgt cgaggacgag gcgggtcgtg
     2581 atggccttct cggcggaggg cctccattcc tcgaccaggc ccagctgggt cttgccggag
     2641 aatgagagca ggcggtcgag cttgtcgtcg gcgtcgatct gcatctggcg cttgaggttc
     2701 tcgaggcgca tgccgagctc ggcgtcgacc atcgagcggg ggaggtcggc cttggccctc
     2761 tcaaggaggc cgtcgacgat cgccttctcc ttgagctggc gcagcttgtc agccaggcgc
     2821 ttctcgaggt gcttccggat gtccgccttg aggtcggcga gggtcttgta cttctccgag
     2881 acgtcctggg cgaagtcgtc gtcgagctcg gggagcttct tctccttgat cttcgtgagc
     2941 ttcacctgga tgctcacggt cctgccggcg agctccttgt actcgtagtc agcggggaag
     3001 gccttctgga ccttcttctc ctcgtcgcgc ttcatgccga cgatctcgtc gtcgaacttg
     3061 tagaggttgt agcccgtccc gatctcgaag gtgaagtcct gacgctcgga cccggcgacg
     3121 gcctggccgt cctccccgag ctcggagtag ttcagggtca cgacgtcgcc cttcgcggct
     3181 gcggccgacg agtccttctc gacgacgatg gcgttgcgct cgcggatgtc ggcgagctcc
     3241 tgggcctcat cctcctgggc gatctcgccc tcggggatct ctatctccat gcccttccag
     3301 tccgcgatct cgaccgtggg gaagacatcg tagatcactg cgaaggagaa gtccttgtcc
     3361 accgagaagt cgggctcgcc gtcgagcctc gggctggagt aggcgagggg cttctcctcg
     3421 atgtcggtga cggcctcctc gacggccttc tccataacgc ggcccatcgc gtccagcttg
     3481 agctccccgc cgaacttgcg ctcgaggacg gcctgcggca cgtggccctt gcggaagccg
     3541 tcgatccgca cgttccgcga gtaatcggcg agcatggccg agtattcctt ctgcacctcg
     3601 tccttgggga cgacgacgct cagcttcacg gtggaatgct caagcttctc gacattcttg
     3661 ctggctatca cgggtgtgtt ccttcttaag acaggatggg gcgcaccacg gggcgatgga
     3721 caaagcccga taatgaaaag aggcgatccg ggtgcccgga tcgcctcatt catgaagagc
     3781 gggaaacggg agtcggaccc gcgacatcca ccttggcaag gtggcgctct acccctgagc
     3841 tattcccgca cgtaacaaaa ccctctgacg cacccgggga tgcgaggaaa gggactcgaa
     3901 ccctcacgcc ctgcggcacc agatcctaag tctggcgtgt ctgccattcc accatcctcg
     3961 cgtcgcggcg cccttccagg cagcgcgtcg catggtagcg gcggcaacgc cccgctgtca
     4021 atagcgctcc gttcttcgcg gttcaaaagc gggacggctt tcgccgtccc gcttccgttc
     4081 ctcatgagcc gcgcaggcat cgaacctgcg acccgcagat taagagtctg ctgctctgcc
     4141 agctgagcta gcggcccatt gtaccttttg caccgtgcgt ccgacaggaa tcgaacctgc
     4201 gacctacgga ttcgaagtcc gtcgctctat ccagctgagc tacgggcgct tccgaggctt
     4261 tcgcctccga ccggacccct ttcacaggat gcagccgtcg ggtgggtgat gggggttgaa
     4321 cccacggcac ccagatccac agtctggcgc tctaccacct gagctacacc caccgtgcaa
     4381 tcggtaggca gagaatgcca aaacccgaaa taaatgtcaa tgagctttgg gagaaaaaaa
     4441 agaatgattc tgctcaatga ccggccgcgg gccccagggg gccttcggcg ggtcagacga
     4501 ggatggggca gctgcgcccg cagctcgcgc agctcgcccg ggacccccgc gggccacctc
     4561 aatcgccgct ggccccaaac ccggcacgct taccgcgtat ccgcggcgcc tcacgaggag
     4621 ggcaccgcag cccgcgcagc gggtatccga ttcgatgccc gacaggttgc cgacgtatac
     4681 gtagcgcaga aagcggcggg ccacgagggc gagctcgagg agggcatcgg ggtcggtggc
     4741 gggggcccgg tgacgccagg ccgggtgata ggccgagagg tggagtggga tgtcgggatc
     4801 gagatcggcc aggaaggctg cgatggcggc gacatcctca ctcccgtcgc tgagatcggg
     4861 cacgacgagg gtagtcacct cgaggtggca tcgggctgag gcctcgcgga tgaagccgag
     4921 gacggcgtcc agatcgccgc cgagctcctt cctgtagcgc tcggccgacc agcatttcag
     4981 gtcgacgttc gcagcgtcgg tcatggcaag gagctcggcc gccgggccgg cctgcaggca
     5041 gccgttggtc accaggatgt tccgtatgcc cgactccctc gcgaggcgca tgcagtccag
     5101 gacgaactcg tagtggacgc tgggctccga gtatgtgtag gcgatcgacg gagaccccga
     5161 cctcctggcg gccttgatga catcctcggc ctccatccgt ttggccaccg actcggtgga
     5221 ctgggagatg tcccaattct ggcagaaggg gcagtggagg ttgcagccga cgaaacccag
     5281 ggagaagacc tgggtcccgg gcaggaagtg gtagaggggt ttcttctcga cgggatccat
     5341 gcccgcgccc gagatcgctc catagaaggg caggacgagc ctgcccccct cgttgaccct
     5401 gaccctgcag atgcccttct tcccggggcc gatgcggcag cggtgcgggc agaggaggca
     5461 ggagatatcc ccgccggcca gcggcttcca gaactccgcc ggcctggccc cccccagggg
     5521 cccctcatcc atctcgcctg cccccgattc cgccatcacc gcccacgggg ggctagcgcg
     5581 ctccgcccat gaagcgctcg cggatgtgcc ccggctccgc ggcgaacctc agcggctcgt
     5641 cgacctcgaa gggctcgtaa cggatgccga agctccccct ccgcacccag agctttatca
     5701 ggggcctcgg ggtgccgagc tcgttgacgg gggctggcat ttcgacagcc gagaggacgt
     5761 cggcgatcct gaactcgaag atggcgggct cgcggtcggc ggcgtcgccg atgagcaggc
     5821 acttgtcctc gtcgtagggg tgcttcctta tcgttcccgt gaaggccacg gcgtcctggg
     5881 gcgactcggc ctggtggtaa cggatgatct ccgaaaaagg ctcagccgag aggtattcgg
     5941 ttatgctcat gcgttctcct ccttcggacc gatcctcggt cgtgctccct tcccataagt
     6001 ataatcgacc atgtcgcgga aagtaacacc agcagggcgg cgaagaggtc gccatggctc
     6061 tcgtagaagg tcggcgccgc cgtccggtag acggggacct cgacgatcct cgagctcgcc
     6121 tcgaagaggg gcatggccgc gagcagctcc ccataggggc cgacgacgca ggagagcccg
     6181 ccgttggtcg agcgcacgag cgtgcgccgg aactctatgg aacggaaacg ggcggccgcc
     6241 cagtgctgga tctccgcgct taccgtcttg gaccaggaat cgttcgtgag gttgatgaga
     6301 aggtcggcgc cggagaggaa gaagggccgg cacacctcgg ggaaggcgtc ctcgaagcag
     6361 atcggcgtgc cgaagctgaa gctgccgccc cgggtctcga gcctgaacag ggtgtagtcc
     6421 ttgcccaggg cccagcccga atcgaggccg accaccttct gcacgaaatt ccggaaccag
     6481 gcgaggtccc agaaggggat cgcctcggcg aagggcacgg gatggatctt cgcgtagctc
     6541 gcgacgagcc tcgggtcggc cccgatgagg aggacggagt tggtcgcggt gaaggtcttc
     6601 cagtcggtca cgacgggagc gcccgtcagg agggggacgc cggtcttggc gatgaagggt
     6661 atcagggggt ggccggcggg ctcggtgctg aagtactggc ggaactcggc gtaggggcgc
     6721 ctgaggctcg tctcgctgaa gacgacgatg tcgggcttcg cgccgccctc ggcgagggcc
     6781 ctctcggcca gctccacatt ggccgacagc cccgcctcct cgccgacaag ccagggatcg
     6841 ttgttctgct ggaccagcag ggcacggaag ctcccgatct cgggaatccg tatgcccatg
     6901 cgggcccatc catagccggc cgtgaggagg atggccaagg ccgccgcgac caggtagccc
     6961 cgcatttggc ggccggtaag cggattccgg ctgagggtcc tgtggtggag gaggctcccc
     7021 tcagacctcg agatcatggg cgaaggcttg ccggcgagga gctcggccag ggatgcgttg
     7081 gcgaaggcga ggagggcgct gatgccgtag acccccgtga cgtcggcgat ctggaggagg
     7141 ggcaggacgc ccgtgaggga ataggggatg aggccccatg gatagccaag gaagccgttc
     7201 gacttcaggt actcgaagcc cgcccacatc gcggcgaagg cgagggggcg gccagccccg
     7261 ctccttctga ggatgaggcc cagatagagg ccgaggacgg agtagacgac agcgtacgcg
     7321 gccgtggaga tgccgatggt ccagaaggcg aaatcctgga agaaatagag ccaatagctc
     7381 gacatcgcat gctggcaggc gccgaagagg ccggtgacga gggcggccat gccgtagctc
     7441 cggcatccgc ggagggcgac gtagaggggg atcagggcca cgaggccgag gaggggcatc
     7501 ccgaggcgga agacctcgtt cggcagaccg aggctcaaaa ggaaggatga agacagccca
     7561 agggcgagga gggtggcggg atggctgctg tggcggggct tctggcccgg ggcgtgccag
     7621 tcttcggggc ttgtgcgcat gccggaagac taatcggtgg gggcataaat gggaagggca
     7681 gggtctcgaa gcgcggccgc cgccggatcc ggttcttgac cccccccgct cagggcacta
     7741 gagtcttgcc atggtcagag ccgtcttccc ggggtccttc gatccgccga ccttcggcca
     7801 cctcaacatt gtggagcgcg cccggagcat cttcgaggag ctcttcgtcg tcgtcgccgt
     7861 gaaccggcag aagagcggct tcttcagccc cgaggagcgg agggacctcc tcctcgagct
     7921 cctcgcgccc tatcccaacg tctcggtgat gctctgcgac accctgatcg tcgatttcgc
     7981 caagaaacac gactgcaagg tcctcgtcag gggggtaagg agcgtgcccg acttctccta
     8041 cgagttcgag ctctccatca tgaacaaggg cctcgatccc tccatagaga ccatcttcat
     8101 gcccaccgac ccgcggttct tcgtcctgcg ctcaagcgcc ataaaggagc tcgcctcctt
     8161 ccacggcgac ctgtcagcca tggtgccgcc aagcgtggtc aaggccctca aggccaaggt
     8221 cggcccctag ccggccgttg gcgggcttcc ggccttgaca attccgggga ctctgtgtag
     8281 ggtacgaagt cggcatacca tacgcagaaa ttctaatggg aatgaggttt tagcaaatgg
     8341 ctgtacc
//
LOCUS       DASSQV010000415         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1217456, whole genome shotgun sequence.
ACCESSION   DASSQV010000415 DASSQV010000000
VERSION     DASSQV010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217456"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFL04_07245"
     CDS             complement(<1..644)
                     /locus_tag="VFL04_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7839536.1"
                     /translation="MKREEGSVFVGPDGEEAIKREYRRVLSAWPLPWEGLEFDTAIGR
                     TFGLACGDREARPLVLLHGTLSNSSIWYADIAALAAGRRVYALDLPGEPGFSHPYRAS
                     WEDGSYAAWLAESLDRLGLERSDIAGISLGAWVALSLAAREPRRAGRLALLCPAGLAP
                     PRASLIPRAILASLGGRRGLEALTRALYGAMPAPEGAVRVGAMLSRHARPRYSSP"
     gene            complement(641..1213)
                     /locus_tag="VFL04_07250"
     CDS             complement(641..1213)
                     /locus_tag="VFL04_07250"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7839537.1"
                     /translation="MSIKYAILGFLSWRPFTGYELKKQFADSLSFHWSGNNNQIYGAL
                     IQLHKEGAVTIEVQPQEKYPTRKVYAITELGRQWLRGWLLGEAELPEERNLVHIQLAW
                     AEALDGGELAALLGRYERVLEDQIIMCRERLRRGAPEPARSPRESLIWRRIDENRVSS
                     YEAELAWARSLASEVAGLGAGHGGVREVAG"
     gene            complement(1370..>2114)
                     /locus_tag="VFL04_07255"
     CDS             complement(1370..>2114)
                     /locus_tag="VFL04_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7839538.1"
                     /translation="RVAARLVEELGGEAVEAGSGEAAIAELRRRRFDLVLMDCMMPGM
                     DGFEATARIREPGSGVLDPGLPIVALTARTQAEDRARCLASGMADYIAKPLTSLGLRQ
                     VLVRLAAATVASGAQEATGRSFDPAEFASRYEGAPELAAEIIGLFLEQSLPLCDRAAE
                     CLRVGDLEGFASALHRLKGSAGAIGAPGVADSAEALLGMARGLGEGADSGCLAPGMEA
                     LGCELKALETELRGLASALGRPEATPKGG"
BASE COUNT          313 a          747 c          771 g          283 t
ORIGIN      
        1 ggcgaggagt agcggggccg ggcatggcgg gagagcatcg cgcccaccct gaccgcgccc
       61 tcgggagcgg gcatcgcgcc gtagagggcc ctggtaaggg cctcgaggcc gcggcggccc
      121 ccgagcgagg cgaggatggc cctcgggatg agggatgccc ttggcggggc caggccggcg
      181 gggcagagga gggcgagcct gcccgccctc cggggctccc gcgccgcgag ggaaagcgcg
      241 acccaggccc cgagcgagat gcccgcgatg tcggagcgct cgagtccgag ccgatccagg
      301 gactcggcga gccaagccgc atagcttccg tcctcccagc tcgcccggta gggatggctg
      361 aagcccggct cccccggcag gtcgagggca tagacccggc gccccgcagc gagggcggcg
      421 atgtcggcgt accagatcga gctgttcgag agggtcccgt ggaggaggac gaggggcctg
      481 gcctcccggt ccccgcaggc gagcccgaag gtcctgccga tcgccgtgtc gaactcgagg
      541 ccctcccagg gcaggggcca ggccgagagg acgcgccggt actcgcgctt gatggcctcc
      601 tcgccgtccg ggcccacgaa caccgagcct tcctcgcgtt tcatcccgct acctccctga
      661 cgcccccatg gccggccccg aggccggcga cctcggaggc gagggacctc gcccaggcca
      721 gctcggcctc gtaggacgag acccggttct catcgatccg gcgccagatg aggctctccc
      781 gggggctccg ggccggctcg ggggccccgc ggcgcagcct ctccctgcac atgatgatct
      841 ggtcctcgag gacgcgctca tagcggccga ggagggccgc aagctcgccc ccatcgaggg
      901 cctcggccca ggccagctgg atgtggacga ggttcctctc ctcgggcagc tcggcctcgc
      961 cgaggagcca gccgcggagc cattgcctgc ccagctcggt tatggcgtag accttgcggg
     1021 tcgggtactt ctcctgcggc tggacctcga tcgtcaccgc cccctccttg tgcagctgga
     1081 tgagggcccc gtagatctgg ttgttgttgc ccgaccagtg gaaggagagg gaatcggcga
     1141 actgcttctt gagctcgtag cccgtgaagg ggcgccagct caggaagccg aggatcgcat
     1201 acttgatcga catggcttcc tcctatacta ggtttatcgg aaactatgtt agaaataaca
     1261 cagttccgcg tacccgtcaa ggggatcagc cttggccgca gggcaatgaa aaaggcccgc
     1321 gcggagcggg ccttcgaggg cggggggcga agccccggtg cggcttcggt cagcccccct
     1381 tcggggtcgc ttcgggcctc cccagcgccg aggccaggcc gcggagctcg gtctcgaggg
     1441 ccttgagctc gcatcccaag gcctccattc cgggcgcgag gcagcccgag tcggccccct
     1501 ctccgaggcc gcgggccatg cccaggaggg cctcggccga atcggccacc ccgggcgcgc
     1561 cgatggcccc ggccgagccc ttgagccggt gcagagccga ggcgaagccc tcgaggtcgc
     1621 cgaccctgag gcattcggcc gccctgtcgc aaaggggcag ggactgctcg aggaagagcc
     1681 cgatgatctc ggcagcgagc tcgggggcgc cctcgtagcg ggaggcgaac tccgccggat
     1741 cgaaggagcg gcccgtggcc tcctgggcgc ccgaggccac cgtggccgcg gcgagcctga
     1801 ccaggacctg cctcagaccc aggctcgtga ggggtttggc gatgtagtcg gccatgcccg
     1861 acgccagaca gcgggccctg tcctcggcct gggtcctggc ggtgagggcg acgatgggca
     1921 ggcccggatc gaggacgccc gagccgggct cgcggatgcg ggccgtcgcc tcgaagccgt
     1981 ccatgccggg catcatgcag tccatgagga cgaggtcgaa gcggcgacgc cggagctcgg
     2041 cgatggcggc ctctcccgaa ccggcctcca cagcctcgcc gcccagctcc tccacgagcc
     2101 gggccgcgac gcgc
//
LOCUS       DASSQV010000416         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_473466, whole genome shotgun sequence.
ACCESSION   DASSQV010000416 DASSQV010000000
VERSION     DASSQV010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473466"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFL04_07260"
     CDS             <1..189
                     /locus_tag="VFL04_07260"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HET7839539.1"
                     /translation="LSRCFGIDLRTAIYATAPGGLSGMALTAADAGADSAVSMLFQLF
                     RLVLILVATPILAALALR"
     gene            complement(202..1065)
                     /locus_tag="VFL04_07265"
     CDS             complement(202..1065)
                     /locus_tag="VFL04_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7839540.1"
                     /translation="MGGKDMKGKVCLVTGGSSGVGLAIASGCARLGATVAIASRDRTR
                     GEAAAKALRDETGNREVEWLGADLANLDSVRSLAEGFGRRYGGLHVLSNNAAVLSMKR
                     RTSAQGFELTFATNYLAHFLLTALLLPMLKAAAPARVITVSGHPASIVGARLDFGDLM
                     LERGYSPIRATLGAALARVLFSLELARRLEGRGVSSNTFHPGLVRSGLPASLPWALRL
                     PARVGMVFLSRECATGVRLACSEEVEGITGRFFVGAKSARFEPSYDLAAEALRLWRAS
                     EALVGQGVAGG"
     gene            complement(1077..1793)
                     /locus_tag="VFL04_07270"
     CDS             complement(1077..1793)
                     /locus_tag="VFL04_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839541.1"
                     /translation="MVPRRLFIALAAAAALAGSAWAQARIEVDVQAGPAFMHRARFGL
                     ILVKTRPQMAIWLETAGGRFLDTIFVTHRSAVADWYGGKGVRRPEALPVWSHARGIPA
                     PDGLFMPDRAHPLADAVSGPTPEAGFTRTWLPPSPLKPGSYLVRVELNQSFDWNEAWP
                     DRLPKDDRRWTEANGQPSIVWEGVLEIRDTAAPAEAVLSPVGTGSLRGEDGGLRRGLE
                     GITTARDIAASIGARYIPGD"
     gene            1864..2508
                     /locus_tag="VFL04_07275"
     CDS             1864..2508
                     /locus_tag="VFL04_07275"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET7839542.1"
                     /translation="MECELRCQRRPKAMTPALPRTVAILVFDEVEILDFAGPYEVFSV
                     ASELLRPSPFFVFAVGLTTQPALGRGRLMVTPRYSIADCPRPDILVIPGGQGTRPLLR
                     HEALLAWIAGCAEGAELCLSVCTGALLLGAAGLLKDRAATTHHGAFETLAALSPSTEL
                     RRDRRFVEATPKIITSGGISAGIDMSLHVVERLLGAQSRAMVEEEMEYGRFAAP"
BASE COUNT          356 a          948 c          887 g          394 t
ORIGIN      
        1 ctctcgcgct gcttcggcat agacctcagg acggcgatct atgccacggc tccggggggc
       61 ttgagcggca tggccctcac cgccgccgac gcgggggccg attcggcggt gtcgatgctg
      121 ttccagctct tcaggctcgt cctcatcctc gtggccacgc ccatcctggc ggccctcgcc
      181 ctgcgctgag ccgggcctgg ctcagccccc tgcgacgccc tgtccgacga gggcctcgct
      241 cgcccgccac agccgcaggg cctcggcggc gaggtcgtag ctgggctcga agcgggcgct
      301 tttagcgccc acgaagaagc ggcccgtgat cccctcgacc tcctctgagc acgcgagcct
      361 gaccccggtc gcgcattcgc ggctgaggaa gaccatgccg accctcgccg gcagcctgag
      421 ggcccagggc aggcttgcgg ggagccctga gcggaccagg ccggggtgga aggtgttgga
      481 gctcactccc ctgccctcga gcctccgagc gagctcgagg ctgaagagca cccgtgccag
      541 ggcggccccc agggtggccc tgatcgggct gtagccccgc tcgagcatga ggtcgccgaa
      601 gtcgagcctc gcgccgacga tgctggccgg gtggcccgag acggtgatga cccgcgccgg
      661 agccgccgcc ttgagcatcg gcaggaggag ggcggtgagg aggaagtggg cgaggtagtt
      721 cgttgcgaag gtcagctcga agccctgggc cgaggtcctc cgcttcatgg agaggactgc
      781 cgcgttgttc gacagcacat ggaggccccc gtagcgtctg ccgaagccct cggccagtga
      841 gcgcacggaa tcgaggttgg ccaggtcggc gccgagccat tcgacctccc tgttgccagt
      901 ctcgtcccgc agggccttgg ccgccgcctc gcccctcgtc ctgtcccggg aggcgatggc
      961 cacggtggcc cccagacggg cgcagccgga ggctatggcc agcccaacgc ccgaggagcc
     1021 gcccgtgacg aggcagacct tccccttcat gtccttgccg cccatcctcg cctcccctag
     1081 tcgccgggga tgtagcgcgc cccgatcgag gccgcgatgt cccgcgccgt ggtgatgccc
     1141 tcgaggcccc tgcggaggcc tccgtcctcg ccccgcagcg agcccgtccc gacggggctg
     1201 agcaccgctt cggctggggc ggccgtgtcg cgtatctcga ggactccctc ccagacgatg
     1261 gagggctggc cgttggcctc ggtccagcgc cggtcgtcct tcggaagccg gtcgggccac
     1321 gcctcgttcc agtcgaagct ctggttgagc tcgaccctta ccaggtagct gcccggcttg
     1381 aggggggagg gcggcagcca ggtccttgtg aagccggcct caggggttgg ccccgagacc
     1441 gcgtcagcca ggggatgggc cctgtcgggc atgaatagtc cgtcgggcgc ggggatgccc
     1501 cgggcgtggg accagacggg gagggcctcg ggcctgcgga cgcccttgcc cccgtaccag
     1561 tcggccacgg ccgatcggtg ggtcacgaag atggtgtcga ggaagcggcc tccggccgtc
     1621 tcgagccaga tcgccatctg gggcctcgtc ttcacgagga tgaggccgaa acgggcccta
     1681 tgcatgaagg ccggccccgc ctgcacgtcg acctcgatcc tcgcctgggc ccaggccgag
     1741 cccgccaggg cggccgccgc cgccagcgcg atgaagagcc tcctcgggac catgcccacc
     1801 tccgcgaaac ccggcctggg ccggcagatt tggcgcttgc gcgccttccc ggacgaatgg
     1861 tacatggaat gtgaactccg atgccagagg aggcccaagg ccatgactcc cgccctcccg
     1921 cgtaccgtgg cgatcctcgt cttcgacgag gtcgagatcc tggatttcgc cggcccctac
     1981 gaggtgttca gcgtcgcgag cgagctcctg cggccgagcc ccttcttcgt ctttgccgtc
     2041 ggcctcacga cccagccagc cctgggccgg ggcaggctga tggtcacgcc ccgctactcg
     2101 atcgccgact gtccgaggcc cgacatcctc gtcatccccg gcggccaggg tacgaggccc
     2161 ctcctcagac acgaggcgct cctcgcctgg atcgcgggct gcgccgaagg ggccgaactc
     2221 tgcctctcgg tctgcacggg agccctcctc ctcggggcgg ccggcctcct caaggacagg
     2281 gccgcgacca cccatcacgg ggccttcgag accctggccg ccctctcccc ctcgaccgag
     2341 ctgcgccggg accggcgctt cgtcgaggcc acgccgaaga tcatcacctc gggcgggatc
     2401 tcggccggaa tcgacatgtc cctccacgtc gtggagcgcc tgctcggcgc ccagtctcgg
     2461 gccatggtcg aggaggagat ggagtacggc cgcttcgccg ccccctgatc cccaacgacc
     2521 cgccccgatc ggggtatatt tggggcgagg aggccctcca tggaactcgc gggcaacaag
     2581 gtcct
//
LOCUS       DASSQV010000417         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_970858, whole genome shotgun sequence.
ACCESSION   DASSQV010000417 DASSQV010000000
VERSION     DASSQV010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970858"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFL04_07280"
     CDS             <1..227
                     /locus_tag="VFL04_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839543.1"
                     /translation="AGLWVAEPSWQAGLSGELKFDAASMSPSWPATLCLELRGMPLAV
                     PFVASAALILSAGPGSSPSWNLGLGIGLIF"
     gene            323..1642
                     /locus_tag="VFL04_07285"
     CDS             323..1642
                     /locus_tag="VFL04_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7839544.1"
                     /translation="MTRDEIIDAAFRAWGVDGFRTNSLSAVSAELGVTKPALYKHFRA
                     KEELLEAVSVRFFDRYSGILKEAFSRGRGGGAAGAPAAAGSTASIDGVLDIFLGMFAD
                     DWVGFISYLSVVLGLPKPHERIRTELEARGVSIPKSPLFGSGPTLLTHFALSSGLFSL
                     ALFHGSRADRSQRPGPEAAAAAIGAARSLVHGGIAWSQGISSDYPLIEARARIGEHER
                     GEVHRLLGAIAAAVAEAGPWKASMDLVAKKAGMSKSGIYAHFKDRDEMLASMFETEYE
                     RIASVLGPRIEACEGLPDRLYAAMATTAEYLIGRPEVLAALDWVRAQRIDLRVAFPER
                     ILAHYRFLEREAEAGRCRLIEGSLDLTLRWIHFLVVNYLVHGSWQEGGMRGGMASLPR
                     LHRLILGGIDMEPPGGIDMEPPGGIDAEALGGAETAASRGSDMEISA"
     gene            1837..>3390
                     /locus_tag="VFL04_07290"
     CDS             1837..>3390
                     /locus_tag="VFL04_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7839545.1"
                     /translation="MTSQRISAAPQSRALLGLVLLAASSALWAQVHPGAQTGPSGTDL
                     RRAAAAPYGNPGTPASSWSTAVPADLGPAALPAEAGPAPRSLGVEDAVNLALDRNLGL
                     AGARLDVAVKQRKAESSWNQFLPQVDVNTTLSRFNMAQSSTVLMPANGYIPTTTIPNI
                     GTAQGLYSDVYYMPIALPTWNLSASLSAQLVLNAAMLESMKGARIDYEAGLISYASAT
                     RKLERDVRKSFYGLLLLQENMELMQENIDNAKRRVDQAEANYKAGLVPELSLLSAQVA
                     WENMKPALDEMRVAYASALDGFALILGLPRGSELQLKGEILPSYAELDAGRLISDRLG
                     DRFDVQGLVKTLEGLKSGEKQLHAQLFTPSLILGWNYDPTFMGDPFKDDLFKGNPTLT
                     QGGGDAWQQRTGMFRATLAFRLNGLFPKSQEYQGLLAMRDGIEQARTGLALTIRGAEA
                     EIDSLVRRLDKSRKSSDALKLNVDLADRAYRLSDLAYKAGAKDLLEVQSAELELRKAQ
                     VQVITENYNY"
BASE COUNT          570 a         1253 c         1044 g          523 t
ORIGIN      
        1 gggccgggct ctgggtggcc gagccctcat ggcaggccgg cctctcgggc gagctgaagt
       61 tcgatgccgc ctcgatgtcc ccctcctggc cggcgaccct ctgtcttgag ctccgcggca
      121 tgcccctcgc cgtccccttc gtcgcgagcg cggccctgat cctcagcgcc ggcccgggct
      181 cgagccccag ctggaacctt ggtctgggca tcggcctgat cttctagccc caccctggtt
      241 ccggccaaca agcttcgagg aactcacaga ggattgacac tctgcagaga tgcacagtag
      301 gttaacgacc ggtcgctaac caatgacacg agacgagatt atcgatgccg ccttccgggc
      361 ctggggcgtc gacggattca ggacgaacag cctctccgcc gtcagcgccg agctcggcgt
      421 caccaagcca gccctctaca agcacttcag ggccaaggag gagctccttg aggccgtctc
      481 ggtccgtttc ttcgaccggt acagcgggat cctgaaggaa gccttctcgc gcggccgagg
      541 cggcggcgcc gcaggagccc cggcggccgc cgggtccacc gccagcatcg acggtgtcct
      601 cgacatcttc ctcgggatgt tcgccgacga ctgggtgggc ttcatctcct acctctcggt
      661 agtcctcggc ctgccgaagc cccacgagag gatcaggacc gagctcgagg cgaggggcgt
      721 cagcatcccc aagagccccc tcttcggctc gggccccacc ctccttaccc atttcgccct
      781 gtcatcgggc ctcttctccc tcgccctgtt ccatggctcg agggcagacc gcagccagag
      841 gcccgggccc gaggccgcag cggccgccat cggcgccgcg aggtcgctcg tccacggggg
      901 catcgcctgg agccagggca tctcgagcga ctatcccctc attgaggccc gggccaggat
      961 cggggagcat gagcggggcg aggtgcaccg cctcctcggc gcgatagccg ccgccgtcgc
     1021 cgaggctggg ccctggaagg cgtccatgga cctcgtcgca aagaaggcgg gcatgtcaaa
     1081 aagcggcatc tacgcccact tcaaggacag ggacgagatg ctcgcctcga tgttcgagac
     1141 ggagtacgag cgcatcgcct cggtcctcgg cccgaggata gaagcctgcg agggcctgcc
     1201 cgaccgtctc tatgccgcga tggccacgac ggccgagtat ctgatcggca ggcccgaggt
     1261 cctggccgcc ctcgactggg tcagggccca gaggatcgac ctcagggtgg cctttcccga
     1321 gcgcatcctg gcccactacc gcttcctcga gcgcgaggcc gaggcgggac gctgccgcct
     1381 catcgagggc tcgctcgacc tgaccctccg ctggatccac ttcctcgtcg tcaattacct
     1441 cgtccacggc tcctggcagg aggggggcat gcgtggcggc atggcctcgc tgccgaggct
     1501 ccaccgcctg atcctcggtg gtattgacat ggagccgccc ggcggcatcg acatggagcc
     1561 gcccggcggt atcgacgccg aggccctcgg cggcgccgag acggcggctt cccgaggaag
     1621 tgatatggaa atctccgcat gaaaccggtt tcagttgccg cggcccgcgg cgcgtcgtat
     1681 attcctttcc gtgcgttcgt aaacgctacg aacgaccgga tttatcatgg acgggatcag
     1741 ggcgcattgc gcggttcccg ccaccgagat ggtcctccga ttgcccggcg catcaagcgc
     1801 gcgcatcagg agctggaccg agaaggagcg ttacccatga cctcacagcg catctccgca
     1861 gcaccccaaa gccgggcctt gctcggcctc gtcctattgg cggcctcgtc cgccctctgg
     1921 gcccaggtcc acccgggcgc ccagaccggg cccagcggca ccgacctgag gagggcggcc
     1981 gcggccccct acggcaatcc cgggacccct gcctcgtcct ggtcaacggc cgtccccgcc
     2041 gacctcggcc ccgccgccct ccctgccgag gccggccccg ccccgcgcag cctcggcgtc
     2101 gaggacgccg tgaacctcgc cctcgaccgc aacctcggcc tggccggggc ccggctcgac
     2161 gtcgcggtga agcagcgcaa ggcagagagc tcctggaacc agttcctccc ccaggtcgac
     2221 gtgaacacga ccctgagccg cttcaacatg gcccagagct ccacggttct catgccggcg
     2281 aatggttaca taccgaccac gaccataccc aatattggaa cagcacaggg cctctactct
     2341 gacgtgtact acatgccgat cgccctcccc acctggaacc tcagcgcctc cttgagcgcc
     2401 cagctcgtcc tcaacgcagc gatgctcgag tccatgaagg gcgcgaggat cgactacgag
     2461 gccggcctca tctcctacgc ctcggccacg aggaagctcg aacgcgatgt gcgcaagagc
     2521 ttctacggac tcctcctcct gcaggagaac atggagctca tgcaggagaa catcgacaac
     2581 gccaagcgcc gcgtcgacca ggccgaggcc aactacaagg ccgggctcgt gcccgagctc
     2641 agcctcctct cggcccaggt ggcttgggag aacatgaagc ccgccctcga cgagatgagg
     2701 gtcgcctacg cctcggccct cgacggcttc gccctcatcc tcggcctgcc caggggctcg
     2761 gagctccagc tcaagggcga gatcctcccc tcctacgccg agctcgacgc gggccggctc
     2821 atctcggaca ggctcgggga caggttcgac gtccagggcc tcgtgaagac cctggagggc
     2881 ctcaagtcgg gcgagaagca gctccacgcc cagcttttca ccccgagcct catcctcggc
     2941 tggaactatg accccacctt catgggcgac cccttcaagg acgacctctt caagggcaac
     3001 cccaccctca cccagggcgg gggcgacgcc tggcagcagc gcacgggcat gttccgcgcg
     3061 accctggcct tccggctcaa cggcctcttc cccaagtccc aggagtacca gggcctcctc
     3121 gcgatgcgcg acggcatcga gcaggcgagg acgggtctgg ccctcacgat ccgcggggcc
     3181 gaggccgaga tcgacagcct ggtgcgcagg ctcgacaagt cccgcaagag ctcggacgcc
     3241 ctgaagctca acgtcgacct cgccgacagg gcctaccgcc tctccgacct cgcctacaag
     3301 gcgggggcaa aggacctcct cgaggtccag tcggccgagc tcgagctccg caaggcccag
     3361 gtccaggtga tcaccgagaa ctacaactat
//
LOCUS       DASSQV010000418         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_970940, whole genome shotgun sequence.
ACCESSION   DASSQV010000418 DASSQV010000000
VERSION     DASSQV010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970940"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFL04_07295"
     CDS             complement(<1..210)
                     /locus_tag="VFL04_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839546.1"
                     /translation="MPRSKTSPVATLIIIALVLVLLYALNPTSDDFAAWRAAKASSQA
                     TSGGGTGLVQELKKGAGAVAGAMTTF"
     gene            complement(325..1170)
                     /gene="ugpE"
                     /locus_tag="VFL04_07300"
     CDS             complement(325..1170)
                     /gene="ugpE"
                     /locus_tag="VFL04_07300"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HET7839547.1"
                     /translation="MVERSLLRRSAPHIFLILAIFVIVFPIWLVFVASTRTLPEILNA
                     PMPSLPGSHFFDNYRTALTEGSRNLGSPALTMLKNSLIMALGISVGKIVVSLLASFAI
                     VFFDFKLRKFCFWSIFITLMLPVEVRIMPTYKVISQLGMLNSYAGLILPMTVSATAVF
                     LFRQFFMSLPREIAEASQIDGARPMDFFARILVPMTRTPIAAMFVIQFIYGWNQYLWP
                     LLITTKEKYYTLLIGINKMLAGADTQVEWQIVMATTLLAMIPPLVVVIVMQRQFVAGM
                     TDTEK"
     gene            complement(1184..2068)
                     /gene="ugpA"
                     /locus_tag="VFL04_07305"
     CDS             complement(1184..2068)
                     /gene="ugpA"
                     /locus_tag="VFL04_07305"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HET7839548.1"
                     /translation="MAKQYEFPNRGLPYLLVLPQMIVVGLFFFWPAAQALWQSLYVQD
                     AFGITLIFVGFENYIKLFRDPNYTSSLLRTFAFSGLVAFTAMAISLFLAVQANRKIVA
                     STFYKTMLIWPYAVATLIAGVLWLFMFNPTVGVLAYFLKHSFGVDWNHIQNSAQAFLL
                     VTLASSWKQISYNFVFFLAGLQNVPVTLVEAAAIDGAGPNRRFWGIIFPMLSPTTFYL
                     LVMNLVFAFFETFPIIHQMTQGGPNQATSILVYKVYQDGYINLNLGASAAQSVVLMVI
                     VIGLTVVQFRFLERKVTY"
     gene            complement(2159..3466)
                     /gene="ugpB"
                     /locus_tag="VFL04_07310"
     CDS             complement(2159..3466)
                     /gene="ugpB"
                     /locus_tag="VFL04_07310"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HET7839549.1"
                     /translation="MLRITSFLVALVLFAGAAFAEPITIEFWHAMGGKNGENTVAVCD
                     KFNASQSDYKVVPVFKNDYTSAMTGAIAAFRAGTPPAIVQIFEVGTATFMAAKGAYKP
                     VYQLMAENGEKFDPNVYIPAIRGYYSTSDGKMLSMPFNSSTAVVYYNKDAFKKAGLDP
                     EKSPKTWEELYADAKKLMAAGYKNGFTSSWATWIQTEQFLAWHNVAYGTKANGFGGLD
                     SQLLLNNPLLIRHFTDLYKWSKEKVFVYGGREGKPDALFTSGEVPIYFQSIGSYANMK
                     SASKFEVGVGNLPYYADVKTAPQNSIIGGASLWVMGKKSAAEYKGVAKFFTFLSRPEI
                     QAFWHQNTGYLPITKAAYELTKLQGFYKDRPGLELAILQLNNKPTTDFSRGVRFGNLP
                     QIRILEDETWEAILDDKISVKDGFDKIVKEGNALIRDFERVNK"
BASE COUNT          683 a          946 c         1320 g          679 t
ORIGIN      
        1 gaaggtggtc atagctcccg cgacggcgcc cgctcccttc ttgagctcct gcacgagccc
       61 cgtgccgccg cccgaggtgg cttggctgct cgccttcgcg gcgcgccatg cggcgaaatc
      121 atcgctggtc ggattgaggg cgtacaacag cacgagcacg agggcgatga tgatgagggt
      181 ggcgacgggg ctggtcttcg atctcggcat gcagagactc cttggggtcg agatgctcgt
      241 atcgaagggg acgatgcgcc cgtcttcgac gtcggcttcg agtctagcgt gaaacaatcg
      301 gttgtgaaag ccggtccggg tgcgtcattt ctccgtgtcg gtcatgcccg cgacgaactg
      361 ccgctgcatc acgatgacga cgacgagggg cgggatcatc gcgagtaggg tggtggccat
      421 cacgatctgc cactcgacct gggtgtcggc cccggccagc attttgttga tgccgatgag
      481 gagggtgtag tatttctcct tggtcgtgat gagcagcggc cagagatact ggttccagcc
      541 gtagatgaac tggatgacga acatcgccgc gatgggcgtg cgagtcatcg gcaccaagat
      601 gcgggcgaag aaatccatgg gccgcgcccc gtcgatctgc gaggcctcgg cgatctcgcg
      661 cgggagcgac atgaagaatt gccggaagag gaagacggcc gtcgccgaga ccgtcatcgg
      721 gaggatgagg cccgcgtagc tgttcagcat gccgagctgg gagatgacct tgtaggtcgg
      781 catgatgcgc acctcgacgg ggagcatgag ggtgatgaag atgctccaga agcagaactt
      841 gcggagcttg aagtcgaaga agacgatggc gaaggaggcg aggagggaca cgacgatctt
      901 gccgacggag atgcccaggg ccatgatgag cgagttcttg agcatggtga gggccgggga
      961 gccgaggttc ctcgagccct cggtcagggc ggtgcggtag ttgtcgaaga agtggctgcc
     1021 ggggagggaa ggcatcggcg cgttgaggat ctcgggcagg gtccgcgtcg aggcgacgaa
     1081 gacgagccag atcgggaaga cgatcacgaa gatcgcgagg atgaggaaga tgtgcggagc
     1141 gctcctccgc agtagcgaac gttcgaccat gttcgaggcc cctctagtac gtgactttcc
     1201 gctcgaggaa gcggaactgg acgacggtga ggccgatgac gatgaccatc agcaccacgg
     1261 actgggcggc cgaggcgccc aggttcaggt ttatgtagcc gtcttggtag accttgtaga
     1321 caaggatgct cgtggcctgg ttgggcccgc cctgggtcat ctggtggatg atcgggaagg
     1381 tctcgaagaa ggcgaagacc aggttcatca cgaggaggta gaaggtcgtg ggcgagagca
     1441 tggggaagat gatcccccag aagcgccggt tcggacccgc gccgtcgatc gccgcggcct
     1501 ccacgagggt cacggggacg ttctgcagcc cggccaggaa gaagacgaaa ttgtaggaga
     1561 tctgcttcca cgacgaggcc agggtgacga ggaggaaggc ctgggccgag ttctggatgt
     1621 ggttccagtc cacgccgaag ctgtgcttga ggaaataggc gaggacgccc acggtggggt
     1681 tgaacatgaa gagccagagg acgccggcga tgagggtcgc cacggcgtag ggccagatga
     1741 gcatggtctt gtagaaggtg gaggccacga tcttccggtt cgcctggacg gcgaggaaga
     1801 gggagatcgc catggcggtg aaggccacga ggccggagaa ggcgaaggtc ctgaggaggg
     1861 agctcgtgta gttggggtcc ctgaagagct ttatgtagtt ttcgaaaccc acgaagatga
     1921 gggtaatccc gaaggcgtcc tgcacgtaga gggactgcca gagagcctgg gccgcgggcc
     1981 agaagaagaa caggcccacg acgatcatct ggggtaggac gaggaggtat ggcagacctc
     2041 tgttcgggaa ctcgtattgc ttggccatcg gaacctcttt cctgccgggc gcctgtccgt
     2101 caaaagggcc gcctcggcct ctagccgaag cggccccctg tggcgctggg cgccggtgtt
     2161 acttgtttac ccgctcgaaa tcgcggatga gggcgttgcc ctccttgacg atcttgtcga
     2221 agccgtcctt gacgctgatc ttgtcgtcca ggatggcttc ccaggtctcg tcctcgagga
     2281 tgcggatctg gggcaggttg ccgaagcgga cgccgcggga gaagtcggtc gtcggcttgt
     2341 tgttgagctg gaggatggcg agctcgaggc ccggcctgtc cttgtagaag ccctggagct
     2401 tggtgagctc ataggcggcc ttcgttatgg ggaggtagcc ggtgttctgg tgccagaagg
     2461 cctggatctc gggcctggag aggaaggtga agaacttcgc gacgcccttg tactcggccg
     2521 cggacttctt gcccatgacc cagaggctgg ctccgccgat gatggagttc tggggggcgg
     2581 tcttcacgtc ggcgtagtag gggaggttgc cgacgccgac ctcgaacttg gaggccgact
     2641 tcatgttggc gtaggagccg atggactgga agtagatcgg gacctcgcct gaggtgaaca
     2701 gggcgtccgg cttgccctcg cggccgccgt acacgaagac cttctccttg ctccacttgt
     2761 agaggtcggt gaagtggcgg atgaggaggg ggttgttgag caggagctgg gagtccaggc
     2821 cgccgaagcc gttggccttg gtgccgtagg ccacgttgtg ccaggcgagg aactgctcgg
     2881 tctggatcca ggtggcccag gagctggtga agccgttctt gtagccggcg gccatgagct
     2941 tcttggcgtc ggcatagagc tcttcccagg tcttcggcga cttctcgggg tcgaggccgg
     3001 ccttcttgaa ggcgtccttg ttgtagtaga cgacggccgt cgaggagttg aagggcatgg
     3061 agagcatctt gccgtcggat gtggagtagt agccgcggat cgcggggatg tagacgttcg
     3121 ggtcgaactt ctcgccgttc tcggccatca gctggtagac cggcttgtag gcgcccttgg
     3181 cggccatgaa ggtggccgtg ccgacctcga agatctggac gatggcgggc ggggtgccgg
     3241 cgcggaaggc ggcgatggcc ccggtcatgg ccgaggtgta gtcgttcttg aagacgggga
     3301 cgaccttgta gtcgctctgg ctggcgttga acttgtcgca gacggcgacg gtgttctcgc
     3361 cgttcttgcc gcccatggcg tgccagaact cgatcgtgat gggttccgcg aaagccgcgc
     3421 ccgcgaacag gacgagggcc acgaggaacg acgtgattcg caacatctgg aagcctcctt
     3481 ggttaatatg agagtgtttc aaaaaatcgc ggattccggg cccggactga aagtccagac
     3541 cgcggcgacg ccagtgtagt cccgaaaacg tatgcacgca atgagatagt tcccggaaaa
     3601 ttggcccact tgccccctca ggggctcc
//
LOCUS       DASSQV010000419         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1714968, whole genome shotgun sequence.
ACCESSION   DASSQV010000419 DASSQV010000000
VERSION     DASSQV010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714968"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="VFL04_07315"
     CDS             complement(<1..1719)
                     /locus_tag="VFL04_07315"
                     /inference="COORDINATES: protein motif:HMM:NF042647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein A"
                     /protein_id="HET7839550.1"
                     /translation="MAQSIAKGDLALTVDELELEASLGFNPHPDGAEWTADKVLRVLM
                     DARIGGFNQKRAEDLVAKFSRAKLPLTEKVAVGQAPENPVPEEPEWLELAVPEELAQI
                     VSEVLATAPPPQLFKLSYETIRTEKTVRKPAALPFLPPKIEKVVVSERREKRESVYPD
                     MAVIRHGYARKGDRLCVLSQAKPGKAGRTIFGKPLQAKAEDSSFYTGSGILRNKTELL
                     ADEDGLVRIGARWVDLVPIPLHKWEVDVSPDGASYFLSYTPGDMRLAGPSAAEVRERA
                     IELGAPDEGLVDEADLAELLRAAASGGEPLFSRSISKDRDAKIEIQVSPDRLRASLSI
                     WKGRGRGRPLELSALSAAIKASGVRGFRPDQLKKDVLEFYKGPKGELLDYTLAEGRPA
                     GRGKDRALAFALSFLAEDRAAAIRASLEAHPGLGKAVRQLDEFPLSAATRVAQVQLGQ
                     RIGELPAAAAGQSGVDVLGAVIPSLQGNDPVIRVYENIDFSKGQLTSTAAGVLLVGES
                     EGGWRLRVVRLRDASIEVFLAGDAMSASVTLLAEEGLGQGLTIERVIAALNAKGVVSG
                     IEPYSVA"
     gene            complement(1727..2878)
                     /locus_tag="VFL04_07320"
     CDS             complement(1727..2878)
                     /locus_tag="VFL04_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HET7839551.1"
                     /translation="MHIVPIASGKGGVGKSMAAANLSIALAQAGKRVVLADLDLGASN
                     LHLILGVPSPRNGIGTFLVGSDKSTSDFEGVVMETDYPGLRFIPGDAEVPGLANIKSA
                     QKNHLVKRLLSLDADYLVLDLGAGTGSNILDFFLMSSRGVVVTAPTLTATLNAYLFLK
                     NAVFRLLYGAFGTRSKAYEYLESLRKDGSSLQKAYVPAILEAVRELDPERWQTATARM
                     ASFRPRLLMNLLEDPKDADKALKIRRSCKDYLNIDIEHLGVLYRDELQDIALGSRIPI
                     IRYKPQGVLSQGIYRTADKLIQAEDEEDVSLSLAELEGGFRTAELEAEADFGAKIAYV
                     EELLSSGALTTGDLVETVRSQQFEIDKLRRENQFLKHKLTKALGQGFSG"
BASE COUNT          422 a          979 c         1107 g          510 t
ORIGIN      
        1 ggcgaccgaa tagggctcga tgccgctgac cacgcccttg gcgttgaggg cggcgatcac
       61 ccgttcgatc gtcaggccct ggccgagccc ctcctcggcg aggagggtga cggaggcgct
      121 catcgcgtcg ccggcgagga agacctcgat gctcgcgtcc ctgagcctga cgacgcggag
      181 cctccagccc ccctcggact cgcccacgag gaggacgccg gccgccgtcg aggtcagctg
      241 gcccttggag aagtcgatgt tctcgtagac cctgatgacc gggtcgttcc cctggaggga
      301 ggggatgacg gcgccgagca cgtcgacccc gctctgtccc gcggccgcgg ccggcagctc
      361 gccgatgcgc tggccgagct ggacctgggc cacccgcgtc gccgcggaaa gggggaactc
      421 gtcgagctgc ctgaccgcct tacccagtcc gggatgggcc tcgagggagg ccctgatcgc
      481 cgccgccctg tcctcggcga ggaagctcag ggcgaaggcg agggccctgt ccttgccccg
      541 gcccgccggc cggccctcgg cgagggtata gtcaaggagt tcgcccttcg gtcccttgta
      601 gaactcgagg acgtccttct tgagctggtc gggcctgaag cccctgaccc ccgaggcctt
      661 gatggcggcg ctcagggcgg agagctcgag gggccttccc ctgcccctcc ccttccagat
      721 cgagagggag gcgcgcagcc ggtcggggct cacctggatc tcgatcttcg cgtcgcggtc
      781 cttggagatg ctccggctga acaggggctc gccgcccgag gccgcggccc tgaggagctc
      841 ggcgaggtca gcctcgtcga cgaggccctc gtccggcgcc ccgagctcga tcgcccgctc
      901 cctgacctcg gcggcgctcg gtcctgccag gcgcatgtcg ccgggagtat agctgaggaa
      961 gtagctggcc ccgtcggggg agacgtcgac ctcccacttg tgcaggggga tcggcacgag
     1021 gtcgacccag cgcgcgccga tccggacgag gccgtcctcg tcggcgagga gctcggtctt
     1081 gttccggagg atccccgagc ccgtgtagaa gctcgagtcc tcggccttcg cctggagggg
     1141 cttgccgaag atcgtcctgc cggccttgcc cggcttggcc tgggagagga cgcagagcct
     1201 gtctcccttc ctcgcgtagc cgtggcggat gaccgccatg tcgggataga cgctctcgcg
     1261 cttctcgcgg cgctcggaga ccacgacctt ctcgatcttc ggcggcagga agggcagggc
     1321 cgcgggcttc ctgaccgtct tctcggtcct gatggtctcg tagctcagct tgaagagctg
     1381 gggcgggggg gcggtggcga ggacctcgga gacgatctgg gcgagctcct cgggcaccgc
     1441 gagctcgagc cactcaggct cctcgggcac ggggttctcg ggcgcctggc cgacggcgac
     1501 cttctcggtg agcgggagct tggcccgcga gaacttcgcc acgaggtcct cggcgcgctt
     1561 ctggttgaag ccgccgatcc tggcgtccat gaggacgcgg aggaccttgt cggcggtcca
     1621 ttcggcgccg tccggatggg ggttgaagcc cagggaggcc tcgagctcga gctcgtcgac
     1681 ggtgagggcg aggtctcctt tcgcgatgct ctgagccatg ccggtcctat ccgctgaaac
     1741 cctggccaag cgccttcgtc agcttgtgct tgaggaactg gttctcgcgg cgcagcttgt
     1801 cgatctcgaa ctgctggctc ctcaccgtct cgaccaggtc ccccgtcgtg agggcgcccg
     1861 aggagaggag ctcctcgaca taggctatct tggcgccgaa gtcggcctcg gcctcgagct
     1921 cggccgtgcg gaagcccccc tcgagctcgg cgagggagag cgacacgtcc tcctcgtcct
     1981 cggcctggat cagcttgtcg gcggtgcggt agatgccctg gctcaggaca ccctggggct
     2041 tgtagcggat gatggggatc cggctcccga gggcgatgtc ctggagctcg tcgcggtaga
     2101 gcaccccgag gtgctcgatg tcgatgttga ggtagtcctt gcaggagcgg cggatcttga
     2161 gggccttgtc ggcgtccttc gggtcctcga ggaggttcat gaggaggcgg ggcctgaagc
     2221 tcgccatgcg cgccgtggcc gtctgccagc gctcgggatc gagctccctg accgcctcga
     2281 ggatggcggg cacataggcc ttctggaggg aggagccgtc cttgcggagg gattcgaggt
     2341 attcgtaggc cttggacctg gtgccgaagg ccccgtagag caggcggaac acggcgttct
     2401 tgaggaagag gtaggcattg agcgtcgccg tgagggtggg ggcggtgacc acgacccccc
     2461 tcgagctcat gaggaagaag tcgaggatgt tcgagcccgt gcctgcgccc aggtcgagga
     2521 cgaggtagtc ggcgtcgagg gagagcaggc gcttcacgag gtggttcttc tgcgcgctct
     2581 tgatgttcgc gaggccgggg acctcggcgt cgccggggat gaagcgcagc cccggatagt
     2641 cggtctccat gaccacgccc tcgaagtcgc tcgtggactt gtcgctgccg acgaggaagg
     2701 tgccgatccc gttcctcggg gaggggacgc cgaggatgag gtggaggttg gacgcgccga
     2761 ggtcgaggtc ggccaggacg acccgcttcc ccgcctgggc cagggcgatg gagaggttgg
     2821 cggcggccat ggacttgccg accccgccct tgccgctcgc gatgggtacg atgtgcacgg
     2881 ggcctaggcc tcgacccgct ctatgtcgtc cggtcccgta ggcccgttgg tggcgccctt
     2941 ggacgggccg gagggaggaa gagcgccgga ccgccctcgc gcgaagacga ggactgacac
     3001 cgagaagagc tccaccag
//
LOCUS       DASSQV010000420         4571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2089933, whole genome shotgun sequence.
ACCESSION   DASSQV010000420 DASSQV010000000
VERSION     DASSQV010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089933"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VFL04_07325"
     CDS             <1..676
                     /locus_tag="VFL04_07325"
                     /inference="COORDINATES: protein motif:HMM:NF037084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HET7839552.1"
                     /translation="RLETRLGAGADPAKITAEVRAIIAESYKAHRRVVFNGNGYSEEW
                     HREAERRGLPRFRSAVDVIPEFASPAAVELFTRHSVFSREEIESRCVIYLEKYLKQVN
                     IEAGVMVDMSRRSILPAAIASAEDFARAASAMASVGAASSVPELRARKVAELVAAASE
                     DTERLEKALLAAQDIDEPLAQAKAYRESVVPRMETLRAKVDALEKQVAKERWPFPSYE
                     ELLFGL"
     gene            complement(649..1095)
                     /locus_tag="VFL04_07330"
     CDS             complement(649..1095)
                     /locus_tag="VFL04_07330"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET7839553.1"
                     /translation="MKEQGEESGTWTHEIQLEVRDYELDIQGIVNNSVYQNYLEHARH
                     RLLKAIGLDFAELHARGTDAIVTRAEIDYRASLRSGDGFVVLSRVLAHGRLRFIFNQE
                     IRRLPDRELCVEARIVAATLVNGRPAPSEEIRLALERAQRPKRSSS"
     gene            1202..2683
                     /locus_tag="VFL04_07335"
     CDS             1202..2683
                     /locus_tag="VFL04_07335"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7839554.1"
                     /translation="MKILKRIAPFAVIVAAALLLLYLPDIGPERYTDLGTFFRVERSR
                     QALSGIAVAVVAKGSVLYVDAFGHDGAGRTLGPNSQLFIGEGSETFTGLCAMALVRDG
                     RLDLDAPVRRYLPWFGFAQGAGQDVSPRHLLTHTSGVSDGSFDDVHASAQDLTGAVRF
                     LLDAVPLAPPGRQFNALATDYQALGLVMEKVSGERYADLLADLVFRPLGMRGSTARPE
                     DLRRSTPQGFGSFFGMPLQRAQTVRVFGAPSAYVVSTASDLGAYLAYLAAPERQRRPP
                     LPQAAVGRLFQPLVPDIPFGWGWYLQEAGGAGAALHDGSLDGFSSRLVLWPLERNGVA
                     LAATQNSLLQSMLSLPALASGARRIILEGQTERPFPLGRLYILLAVMAAVHLIVLSAQ
                     TGGALRWAKDVKGRADAVGAPGPIRFAVARSILGLALRVAVFSLAPAALGILIHRRLA
                     WGTAFALEPGIAAWFASACFFGALRNVARLAWLRGARSVRRQR"
     gene            2771..3610
                     /locus_tag="VFL04_07340"
     CDS             2771..3610
                     /locus_tag="VFL04_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7839555.1"
                     /translation="MTTEIISRDPEWPYSKAKTAVLTAAASVICESGPRAATLKNIAG
                     RAGITEPAIFRHFEGVDGLFEGLFHAYERVYRRFDEAYHGDERGLARLRTALLSIMDY
                     FAASREFAYILLHAQSVFRGYADLKRKTVEYSAKDRANAVACIAEGIDRGEIRGDADP
                     QSMAVSVLGAMSMTVMTWIESSFSFDLRELAERRWDDIEVLIAKPGTRRLARRKPMPA
                     PLPTPLPAKKEAKVKAKPAALAAPAKKLGAKKLPAKPVAAKKAAPAAKKAAKKPVGPS
                     KRK"
     gene            3615..>4571
                     /locus_tag="VFL04_07345"
     CDS             3615..>4571
                     /locus_tag="VFL04_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7839556.1"
                     /translation="MRRSGPGGLFILEPGLVFLNHGSFGARPRELLEEQDRFRRMAEA
                     EPVRFLARSSGALIEAALGALAAYLGASPRDLAFVENATTAVGIAARSLALEPGDEVI
                     GTDHEYGACAEAWRRVCEARGASYRSIAVPLPYAGHGDFLARLEGAIGPRTRALFVSQ
                     ITSPTALELPAAEVCALARRLGLVSVIDGAHVPGQLELELGKLGADFYAGNCHKWLCA
                     PLGSAFLYVREGLHEGVVPPVVSWGLVDEANGTDFHEAYAGSFPLARRLRWLGTRDIS
                     AFLAVPAAIDFCRRQADEARKCATLAAETARRAAALLGLESLA"
BASE COUNT          682 a         1673 c         1480 g          736 t
ORIGIN      
        1 ccggctcgag acgaggcttg gggcgggcgc agatcccgca aagatcacgg ccgaggtcag
       61 ggccatcatc gccgagtcct acaaggcaca ccgccgcgtc gtgttcaacg gcaacggcta
      121 ctccgaggag tggcaccgcg aggccgaacg ccgcggcctg ccccggttca ggtcggccgt
      181 cgacgtcatc cccgagttcg cctcgcccgc cgcggtggag ctcttcacga ggcattcggt
      241 cttcagcagg gaggagatcg agagccgctg cgtgatctac ctcgagaagt acctcaagca
      301 ggtcaacatc gaggcgggcg tcatggtcga catgtcgagg cgctcgatcc tccccgcggc
      361 gatcgcgagc gccgaggact tcgcccgcgc ggcctcggcc atggcctcgg tcggcgcggc
      421 ctcctccgtg cccgagctcc gcgcccgcaa ggtggccgag ctcgtggcgg ccgcgtcgga
      481 ggacaccgag cgcctcgaga aagctcttct ggccgcccag gacatcgacg agcccctggc
      541 ccaggccaag gcctaccgcg agagcgtggt gccgaggatg gaaaccctca gggccaaggt
      601 ggacgcgctc gagaaacagg tagccaagga gcgctggccc ttcccgagct acgaggagct
      661 cctcttcggc ctctgagcgc gctcgagcgc gaggcggatc tcctcgcttg gagctgggcg
      721 gccattcacc agggtggccg ccactatcct cgcctcgacg cagagctccc ggtcgggaag
      781 ccgccttatt tcctgattga aaatgaaccg gagcctgccg tgggccagga cccgcgagag
      841 cacgacgaaa ccgtctcccg agcgaaggct cgcgcgatag tcgatctcgg ccctcgtcac
      901 gatggcgtcg gtgccgcggg cgtggagctc ggcgaaatcg aggccgatgg ccttgagcag
      961 cctgtgcctc gcgtgctcaa gatagttctg gtatacggaa ttgttcacga tgccctggat
     1021 gtccagctcg tagtccctca cctcgagctg gatctcgtgg gtccacgttc ccgactcctc
     1081 tccctgctcc ttcaccattc ctcctcgcgc tgtcgccgac caaggccccc tccggggccc
     1141 tgcccggagg ccggggattc cggtcaacgc accgcatcct tccatgatat aatcccgccc
     1201 gatgaagatc ctcaagcgga tcgccccctt cgcggtgatc gtggccgcgg ccctcctcct
     1261 gctctacctg ccggacatcg gcccggagcg ttatacggac ctgggcacct tcttccgggt
     1321 cgagcgctcg cgccaggccc tgtcgggcat cgccgtcgcc gtggtcgcca agggctcggt
     1381 cctctatgta gacgcctttg gccatgacgg cgccggcagg actctggggc ccaacagcca
     1441 gctcttcatc ggggaaggaa gcgagacctt cacggggctc tgcgccatgg ccctcgtgcg
     1501 cgacggcagg ctcgatctcg acgcgccggt cagaagatac ctcccctggt tcggcttcgc
     1561 ccagggcgcg gggcaggatg tcagcccgcg ccaccttctc acgcacacga gcggggtctc
     1621 ggacgggagc ttcgacgacg tccacgcctc ggcccaggac ctgacgggag ccgtccgctt
     1681 cctcctcgac gccgtgcctc tcgccccgcc gggcagacag ttcaacgccc tcgccaccga
     1741 ctaccaggcc ctcggcctcg tcatggagaa ggtctcgggc gagcggtacg ccgacctgct
     1801 cgccgatctc gtgttcaggc ccttgggcat gagggggagc acagccaggc ccgaggatct
     1861 gcgccgctcg accccccagg gcttcggctc cttcttcggc atgcccctcc agagggccca
     1921 gacggtcagg gtcttcggcg ctccctcggc ctatgtggtg tccacagcct cggaccttgg
     1981 tgcctacctc gcctatctgg cggcgcccga gcggcagcgg cggcctcccc ttcctcaggc
     2041 ggcggtgggc aggctgttcc agccccttgt ccccgacatc ccctttggct ggggctggta
     2101 tctccaggag gccggcggcg ccggggccgc cctccacgac ggctccctcg atggattctc
     2161 gtccagactg gtcctctggc ccctggagag gaacggcgtg gccctcgccg ccacccagaa
     2221 ctccctcctc cagtcgatgc tctccctgcc ggccctcgcc tcaggggcga ggcggatcat
     2281 cctcgagggc cagaccgagc ggcccttccc cctgggccgg ctctacatcc tcctggccgt
     2341 catggcggcg gtccacctca tcgtcctatc ggcccagacg gggggcgcgc tcagatgggc
     2401 caaggacgtc aagggcaggg ccgacgccgt cggggctccg ggcccgatcc gcttcgccgt
     2461 ggcgcgctcg atcctcgggc tcgccctccg cgtggcggtc ttctcgctcg cccccgccgc
     2521 cctgggcatc ctcatccaca ggaggctggc ctggggcacg gccttcgcgc ttgagccggg
     2581 catcgcggcc tggttcgcct cggcctgttt cttcggggcg ctgcgcaacg tcgcccggct
     2641 cgcctggctc aggggggccc gctcggtccg cagacagagg tgaagcccag atataacatt
     2701 aggctaaata tcatgatgat taattcatta cagacttccc atgaaccgga ttggagggta
     2761 tgattattat atgactacag agataatctc cagggacccc gagtggccct attcgaaggc
     2821 gaagaccgcg gtcctgaccg ctgccgcatc agtcatctgc gaaagcgggc cgagggccgc
     2881 caccctcaag aacatcgcgg ggcgggcggg catcaccgag ccggcgatct tccgccactt
     2941 cgagggggtc gacggcctgt tcgaggggct cttccacgcc tatgagcggg tgtaccggcg
     3001 cttcgacgag gcctaccacg gtgacgagcg cggcctggcc aggctcagga cggccctgct
     3061 ctcaatcatg gactacttcg ccgcgagccg ggagttcgcc tacatcctcc tccacgccca
     3121 gagcgtcttc aggggctatg ccgacctcaa gcgcaagacc gtcgagtact cggccaagga
     3181 tagggcgaac gcggtggcct gcatcgccga gggcatcgat cgcggggaga tccgcggcga
     3241 cgccgacccc cagtccatgg ccgtctccgt cctcggggcg atgtccatga ccgtcatgac
     3301 ctggatcgag tcctcattct ccttcgacct ccgcgagctc gccgagcgca ggtgggacga
     3361 catcgaggtc ctgatcgcca agcccggcac ccgccggctg gcccgccgca agcccatgcc
     3421 cgcgcccctg ccgacccccc tgcccgcgaa gaaggaggcg aaggtcaagg ccaagcccgc
     3481 agccctcgcc gctcccgcca agaagctcgg cgcgaagaag ctcccggcca agccggtcgc
     3541 ggccaagaag gccgcccccg cggcgaagaa ggccgccaag aagccggtcg gcccctccaa
     3601 gcgcaagtag aggcgtgagg cgctccggcc ccggcggcct tttcatcctc gagcccggtc
     3661 tcgtcttcct caaccacggc tccttcgggg cgaggccgag ggagctgctc gaggagcagg
     3721 acaggttccg ccgcatggcc gaggccgagc ccgtgcgctt cctcgcccgg agctccgggg
     3781 ccctcatcga ggctgccctc ggagctctgg ccgcctacct cggcgcatcg ccgcgcgacc
     3841 tcgcctttgt ggagaacgcc accactgccg tcggcatcgc cgcccgctcc ctcgccctcg
     3901 agccggggga cgaggtgatc ggcaccgacc acgaatacgg ggcctgcgcc gaggcctggc
     3961 gccgcgtctg cgaggccagg ggagcgagct accgcagcat cgccgtcccg ctgccctatg
     4021 ccggacatgg ggatttcctc gccaggctcg agggagccat cggccccagg accagggccc
     4081 tcttcgtctc gcagatcacg agcccaacgg ccctcgagct gccagcggcc gaggtctgcg
     4141 ccctcgcgcg gagattgggc ctcgtgagcg tcatcgatgg agcccatgtc ccgggccagc
     4201 tcgagctcga gctcgggaag ttgggcgcgg atttctatgc ggggaactgc cacaaatggc
     4261 tctgcgcccc cctgggctcg gccttcctct acgtgcgcga aggcctccac gagggcgtcg
     4321 tcccgcccgt cgtgtcctgg ggtctcgtcg atgaggccaa tgggaccgat ttccacgagg
     4381 cttacgcggg ttcattcccg ctcgcgaggc ggctgcgctg gctggggacc cgcgacatct
     4441 cggctttcct cgccgtgccc gcggccatag acttctgccg gaggcaggcc gacgaggccc
     4501 ggaagtgcgc gaccctggcc gccgagacgg cccgaagggc cgccgccctc ctcgggctcg
     4561 agagccttgc g
//
LOCUS       DASSQV010000421         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_721123, whole genome shotgun sequence.
ACCESSION   DASSQV010000421 DASSQV010000000
VERSION     DASSQV010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721123"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..855
                     /gene="gpmA"
                     /locus_tag="VFL04_07350"
     CDS             103..855
                     /gene="gpmA"
                     /locus_tag="VFL04_07350"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HET7839557.1"
                     /translation="MHKVVLVRHGESVWNKENLFTGWTDVDLSDKGRTEALEAGRLLK
                     AEGYNFDLAFTSVLKRAIRTLWYLQDEMDLMWIPVFRDWRLNERHYGALQGLNKAETA
                     EKYGEAQVKIWRRSYDIPPPRLDDEDPRHPRRDPRYAGLTPKELPATECLKDTVARFL
                     PAWQNRIAPEIQAGKKVLVVAHGNSLRALVKYLDGVSDADIVELNIPTGIPLVYELDG
                     DLKPVRHYYLGDQAKVEEAMRGVANQAQARRA"
     gene            872..1999
                     /gene="tal"
                     /locus_tag="VFL04_07355"
     CDS             872..1999
                     /gene="tal"
                     /locus_tag="VFL04_07355"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET7839558.1"
                     /translation="MEQNPLKELGRLGQSVWLDYISRELISGGGLRRLIDEDGLCGMT
                     SNPAIFEKAINGSSVYEADIRALAQRGKDVQAIYEELALGDVRAAAEVFAPVHHSSGG
                     LDGYVSLEVDPRLAHDAAGTIAEGRRLWAALDRPNVFVKVPATKEGIAAIRALIAEGI
                     NINVTLLFGIPRYREVIEAYLGGLEERKAAGKPVGSVRSVASFFVSRIDTLVDQMLEG
                     MIAAGGPEKELAASLRGQVAIDSAKVAYLIWKEAFKGQRFGRLGAQTQRLLWASTSTK
                     NPAYSPVKYVEALIGPETVNTMPLETIEAYRAQGQPRSRLELDQAEAGWALRRLAELG
                     IGIDEVTQKLEDEGVDKFIKPFAALLGSIEKAAARAAHPVA"
     gene            2014..3486
                     /locus_tag="VFL04_07360"
     CDS             2014..3486
                     /locus_tag="VFL04_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET7839559.1"
                     /translation="MAQKRKTEDRLGRPPQTGTLPLRAFREESPECMMVVAGSGRFRR
                     MNASAREFLGYTKEELFHLNLDLIGAGSVRDRFGELGKSGRSRIEGSFVKKNGTPVRA
                     VMHAAGVGDGSYQLILAEIAAAREAREAADKALALDLGSTPLLLADLSALRLRLEGLR
                     AGGMRDLEARLRGAPGELAALAALVRPLAMSRSLGELLGRVVAGDRPARIQDLLAGAG
                     GGFAATLSGLWRGGASCESVYALELAEGRRFFRVQLSLEPARADPWSRALLSFVDVTG
                     ERRSLDELEAAVAEKTLLLEELEHRVKNSLNLVASMLGIEARSLHEGECAAPIMAAQA
                     RIRTVASLYERLSEGRDAKHLECAPYLEGIIRKLRETYCPAGGRVRIGTRLAPLRIDA
                     KRAASIGLILNELITNSLKHAFPGEAAGEILVETRLEGDRLGLRVADDGVGLPGCREP
                     AAGRGQGLQFVEMLTRQLCAELEVSSTAGCAYSFSIPLRP"
BASE COUNT          572 a         1262 c         1242 g          486 t
ORIGIN      
        1 cccggtcggc cgccccgcgt gggccccccg cccgccggcc ccgcccgagc cggcgcgttg
       61 gtatattcaa gttatgccgc atgccagggg aggccatgaa tgatccataa agtcgtcctt
      121 gtgcgccacg gggagagcgt ctggaacaag gagaacctct tcaccggctg gaccgacgtc
      181 gacctgtcgg acaagggcag gaccgaggcc ctcgaggcgg gccgcctgct caaggccgag
      241 ggctacaatt tcgacctcgc cttcacctcg gtgctcaagc gcgcgatccg caccctctgg
      301 tacctgcagg acgagatgga cctcatgtgg atccccgtgt tccgggactg gaggctcaac
      361 gagcggcact acggggccct ccagggcctc aacaaggccg agacggccga gaagtacggc
      421 gaggcccagg tgaagatctg gaggcggagc tacgacatcc ccccgccccg gctcgacgac
      481 gaggaccccc ggcatccccg gcgggacccg cgctacgccg gcctcacccc gaaggagctg
      541 cccgccaccg aatgcctcaa ggacacggtg gcgcgcttcc tgccggcctg gcagaaccgc
      601 atagcccccg agatccaggc gggcaagaag gtcctcgtgg tggcccacgg caacagcctc
      661 agggccctcg tcaaatacct cgacggggtg tccgacgccg acatcgtcga gctcaacatc
      721 cccaccggca tccccctcgt ctacgagctc gacggggacc tgaagcccgt caggcactat
      781 tacctgggcg accaggcgaa ggtcgaggag gcgatgcgcg gcgtcgcgaa ccaggctcag
      841 gctcgccgag cctgaaaggc aggtcaggat tatggaacag aacccgctca aggaactcgg
      901 gagactgggc cagtcggtct ggctggacta catcagcagg gagctcatct cgggtggagg
      961 cctccgccgc ctcatcgacg aggacgggct ctgcggcatg acctcgaacc ccgcgatctt
     1021 cgagaaggcc atcaacggca gctcggtgta cgaggccgac atcagggccc tcgcccagag
     1081 gggcaaggac gtgcaggcga tctacgagga gctggcgttg ggcgacgtgc gggcggcggc
     1141 cgaggtcttc gcccccgtcc accattcgag cgggggcctc gacggctacg tgagcctcga
     1201 ggtcgacccc cgcctcgccc acgacgccgc ggggaccatc gccgagggcc gcaggctctg
     1261 ggcggccctg gacaggccca acgtcttcgt caaggtgccg gcgaccaagg agggcatcgc
     1321 ggcgatcagg gccctcatcg ccgagggcat caacatcaac gtgaccctcc tcttcggcat
     1381 cccccgctac cgcgaggtca tcgaggccta cctcggcggg ctcgaggagc gcaaggcggc
     1441 cggcaagccg gtaggctcgg tgcggtcggt ggcgagcttc ttcgtcagcc ggatcgacac
     1501 cctcgtcgac cagatgctcg agggcatgat cgccgcgggc ggccccgaga aggagctcgc
     1561 agcatcgctc cgcggccagg tggccatcga cagcgcgaag gtcgcctatc tgatctggaa
     1621 ggaggccttc aagggccagc gcttcgggag gctcggggcc cagacccagc gcctcctgtg
     1681 ggcgagcacg agcacgaaga acccggccta cagcccggtc aagtacgtgg aggccctcat
     1741 cgggcccgag accgtcaaca ccatgcccct cgagacgatc gaggcctacc gggcccaggg
     1801 ccagccgcgg tcccggctcg agctcgacca ggccgaggcc ggctgggccc tccgccgcct
     1861 cgccgagctc ggcatcggga tcgacgaggt cacccagaag ctcgaggacg agggggtgga
     1921 caagttcata aagcccttcg ccgccctcct cggctcgatc gagaaggcgg cggccagggc
     1981 ggcccacccc gtggcctaaa ccgcccagcg gcgatggcgc agaagcggaa gacggaggac
     2041 cggctcggcc ggcctcccca gacggggacc ttgccgctgc gggccttcag ggaggaatcc
     2101 cccgagtgca tgatggtggt cgcgggctcg gggcggttca ggaggatgaa cgcctctgcg
     2161 cgcgagttcc tcggctacac gaaggaggag ctcttccacc tcaacctgga cctgatcggc
     2221 gccggctcgg tgcgggacag gtttggcgag ctcgggaaat cggggcgctc gcggatcgag
     2281 ggctccttcg tgaagaagaa cggcaccccg gtcagggccg tcatgcacgc cgcgggggtg
     2341 ggggacggct cctaccagct catcctcgcc gagatcgcgg ccgcccgcga ggcgagggag
     2401 gcggcggaca aggccctggc cctcgacctg ggctcgactc ccctcctcct cgccgacctc
     2461 tcggccctca ggcttcgcct cgaggggctc agggccgggg ggatgcggga tctcgaggcc
     2521 cggctgcgcg gggcgccggg ggagctcgcc gccctggccg ccctggtcag gcccctcgcc
     2581 atgagccgca gcctcgggga gctcctcggg cgtgtcgtcg cgggggacag accggcccgg
     2641 atccaggacc tcctcgccgg ggctggcggg ggtttcgcgg ccaccctctc cgggctgtgg
     2701 cgcggcgggg cctcctgcga gtcggtctac gccctcgagc tcgccgaagg ccggcgcttc
     2761 ttccgcgtcc agctgagcct cgagccggcc cgggccgatc catggtcccg ggccctcctg
     2821 tccttcgtcg acgtgaccgg cgagcggcgg agcctcgacg agctcgaggc ggcggtcgcc
     2881 gagaagaccc tcctcctcga ggagctggag caccgggtca agaacagcct caacctcgtc
     2941 gcgagcatgc tcggcatcga ggcgaggtcc ctccatgagg gggaatgcgc ggcgcccata
     3001 atggccgcac aggcccggat caggacggtg gcctcgctct acgagcggct gtccgagggc
     3061 cgcgacgcga agcacctcga gtgcgcgccc tatctcgagg gcatcatccg caagctcagg
     3121 gagacctatt gccccgccgg aggacgcgtg cgcatcggga cccggctcgc ccccctgcgg
     3181 atcgacgcca aacgcgcggc gtccatcggc ctcatcctca acgagctcat cacgaactcg
     3241 ctgaagcacg ccttccccgg ggaggcggcc ggggagatcc tcgtcgagac gaggctcgag
     3301 ggggatcgcc ttggcctgag ggtcgccgat gacggagtcg gcctgcccgg gtgccgggag
     3361 cctgccgcgg ggagggggca gggcctccag ttcgtcgaga tgctgacgcg gcagctctgt
     3421 gccgagctcg aggtctcctc gaccgcgggc tgcgcctaca gcttctcgat ccccctgcgg
     3481 ccctagggcc tggcgttagc gcgccgtctg cccgcgcctc agggacgcgg cccccgtttg
     3541 cgagctctga gcctccaatt tc
//
LOCUS       DASSQV010000422         5018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1963966, whole genome shotgun sequence.
ACCESSION   DASSQV010000422 DASSQV010000000
VERSION     DASSQV010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963966"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFL04_07365"
     CDS             <1..551
                     /locus_tag="VFL04_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HET7839560.1"
                     /translation="HRGIRYLVWGQRLTASDSSRMDLYIARMASPSKLLGKPVMIASA
                     DQAWEMVDPSKLKLEGPAVLQRNGRIFISYSANAIDASYCMGMLEMSQDADPLSASSW
                     TKLPGPVFQSDPAARQFGPGHNSFTSSRDGSVDYLVYHDRNYERIEGYPILDPNRNTR
                     VQAFTWGPDGRPLFGKPVADGM"
     gene            complement(730..1416)
                     /locus_tag="VFL04_07370"
     CDS             complement(730..1416)
                     /locus_tag="VFL04_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7839561.1"
                     /translation="MPDRSASGSVFIIDDHAAVRQGLRAILEDSGRYSVSGAVASAEE
                     AREALAELREAGQAPSILIIDLNLKGQSGIDFVREAARTDPDSRCVMISVSIRFDSII
                     EAMAAGARGYIGKDQDEGSTLRVLDAVARGDLGLDGEVLATVLGNALRLFSAKMGLER
                     SRYDSLTPREKEVFRLTILNRSVGEIASSLDLSTKTIDNVRSAILGKLGVQDRFEMYR
                     YALRIGVIEE"
     gene            complement(1409..2644)
                     /locus_tag="VFL04_07375"
     CDS             complement(1409..2644)
                     /locus_tag="VFL04_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7839562.1"
                     /translation="MQHEAAPAQSTAAQVFIALIALVLVVYVAITWSILGRIEELRSQ
                     SRDFFDRWLECKSEAVLFLATTMRWEPSALVAEFDACDRLFARISAGPHASAARLFQD
                     LGMIDLKPAWEGLRASLGLMGGGDPQGRDPLPRLQAFTALAKGFETAFRTRMAAIDAV
                     AEGQNRALLTLQLSAIAAVLGLAGLSAWISRIAHRAQEGGKRLAELVGATFAAQEAER
                     RRISLDLHDSVAQEVSAALMTARRLAENQEGTRAALIASLKTTIDALRRISWEMRPPE
                     LERFGFQGAAMRLLEAFSERSGLSVEAAPIECDVSGLSESVAMHLYRILQESLMNIQK
                     HAGAKAVRVEISRPGDSVRLVVEDDGCGFDATAQDGAGPAHLGIAGMRERARLIGGLL
                     AVSSAPGRGTRLKVEAPYA"
     gene            2699..3280
                     /locus_tag="VFL04_07380"
     CDS             2699..3280
                     /locus_tag="VFL04_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7839563.1"
                     /translation="MDRASSKRLLVVAALVVAGGLGNALMSLANMALSSPFFFDSVFT
                     AVCAALFGPLAGALCGLVSHLFMELFHGWNGLFLPFAVCNIATGLIVGLFARSGRLAT
                     LTGAMLCTILVTLGNSVLGALVAFYLFGGITGHASDYLVTGFILVGQTLLAAAFWARI
                     PANLIDKGIAVALAYFAARRALGQRAEAARLGR"
     gene            complement(3269..4888)
                     /locus_tag="VFL04_07385"
     CDS             complement(3269..4888)
                     /locus_tag="VFL04_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7839564.1"
                     /translation="MAQGSGSGTDGGKASTGLTLDGIIVFADFQKRLSGFLHDLRQVS
                     DLNSIFVGKVLDSGGAHERSLAEVKGELDRIDSSSRLVLSDLEAADEAVSGSREESMR
                     SLAAMDKTKTSVDSLDATFGSIADLFQTIRGEAKAILDQIARIVDISELTNLLALNAA
                     IQAARAGEHGKGFAVVAKEVRNLAETTKRITAELSQRLIALQKGLDESGSSMERFGAI
                     KGEIIADVRLSSERLGSSSEALSAAASRIAHVRDLSREQSESAGLILGRVARLATDTR
                     FLNSSSSHIVASMASEKEILSALALDASRTSASFTSGGRRAEGRRIRVGHDVTYPPWV
                     YLDKGNSAGVSVDLLRRISAAAGLEIEIVGDEWDRIRDAFENGDIDLVINAGWPNSAF
                     REASVIPTMPYERFEVRVFMHASKLPAEGSLELRGKRIAVQKASYVDQVIASTGCELV
                     YIENDLDGMVKLIWEEVDGAATEAKVGEYLSRKFFGGKIVPATGVLGTKDVVMLARPG
                     SEALRDALDAGIRAVAQGASRGSGPRAGSPA"
BASE COUNT          748 a         1689 c         1742 g          839 t
ORIGIN      
        1 agcaccgggg catccgctat ctggtctggg gccagaggct cacggcctcg gacagctccc
       61 gcatggacct ctacatcgcg cggatggcga gcccctcgaa gctcctcggg aagccggtca
      121 tgatcgcgag cgccgaccag gcctgggaga tggtcgaccc ctccaagctc aagctcgagg
      181 gccccgccgt gctccagcgg aacgggcgga tcttcataag ctattcggcc aacgccatcg
      241 atgcgagcta ctgcatgggc atgctggaga tgagccagga cgccgatccc ctctcggcct
      301 cgtcctggac caagcttccc ggtccggtct tccagagcga ccccgcggcg agacagttcg
      361 gcccggggca caacagcttc acgagctcgc gcgacggctc ggtcgactac ctcgtctacc
      421 acgacaggaa ctacgagagg atcgagggct accccatcct cgacccgaac cgcaacacca
      481 gggtccaggc cttcacctgg ggcccggacg gccggcccct cttcggcaag cccgtcgccg
      541 acggcatgtg agggaatgaa aacggccgcc ccttttccgg gggcggccat cgatgcgtgg
      601 cgtagggcat tttgtatcgt tttgtgattg ttccttcagt gggaccacgg tacgccctgc
      661 cgccgggcca cgcaagaggg aggggggaag acaaaaccgg gatattgacc cgggagggcc
      721 gccggctgcc tattcctcga tgacgccgat cctgagcgcg tagcggtaca tctcgaagcg
      781 gtcctggacc ccgagcttgc ccaggatggc cgagcggacg ttgtcgatgg tcttggtgga
      841 gaggtccagg gaggaggcga tctcgccgac gctccggttc aggatggtga ggcggaagac
      901 ctccttctcc cgcggggtga gggagtcgta gcgcgagcgc tcgaggccca tcttcgcgga
      961 gaaaaggcgc agggcgttgc cgagcacggt ggccaggacc tcgccgtcga ggcccaggtc
     1021 gccgcgcgcc acggcgtcga ggaccctgag ggtcgagccc tcgtcctggt ccttgccgat
     1081 gtagccgcgg gcccccgcgg ccatcgcctc gatgatggag tcgaagcgga tcgagacgct
     1141 gatcatgacg cagcgggagt cggggtctgt cctcgcggcc tcgcggacga agtcgatccc
     1201 gctctggccc ttcaggttca ggtcgatgat gaggatcgag ggggcctggc ctgcctccct
     1261 gagttcggcc aaggcttcgc gggcctcctc ggccgaggcc acggcccccg agaccgagta
     1321 gcggcccgag tcctcaagga tcgccctgag gccctgccgc acggcggcgt ggtcgtcgat
     1381 gatgaacacg gatccggatg cggacctgtc aggcataggg ggcctccacc ttgagccttg
     1441 tcccgcggcc cggggccgag gagaccgcga ggaggccacc gatcagcctc gccctctccc
     1501 gcatccccgc gatgccgagg tgggccggcc cggccccgtc ctgggccgtg gcgtcgaagc
     1561 cgcagccgtc gtcctcgacg acgagccgca ccgagtcccc cggcctggat atctcgaccc
     1621 tgaccgcctt ggctcccgcg tgcttctgga tgttcatgag ggactcctgg aggatgcgat
     1681 agaggtgcat ggccacggat tcgctcaggc ccgagacgtc gcactcgatc ggagctgcct
     1741 cgacgctgag gccgctccgc tccgagaagg cctcgagcag gcgcatggcc gcgccctgga
     1801 agccgaagcg ctcgagctcg ggcgggcgca tctcccagga gatgcggcgc agggcgtcga
     1861 tggtcgtctt gagcgaggct atcagggctg cccgcgtgcc ctcctggttc tcggcgaggc
     1921 ggcgggcagt catgagggcg gccgagacct cctgggccac ggagtcgtgg aggtcgaggg
     1981 agatcctccg cctctcggcc tcctgggcgg cgaaggtcgc cccgaccagc tcggcgaggc
     2041 gcttgccccc ctcctgggcc ctgtgggcga tgcgcgaaat ccaggcgctg aggccggcga
     2101 ggccgaggac ggccgcgatg gccgatagct gcagggtcag gagggcccgg ttctggccct
     2161 cggccacggc gtcgatggcg gccatcctcg tcctgaacgc ggtctcgaag cccttcgcga
     2221 gggcggtgaa ggcctggagc cggggcaggg ggtctcggcc ctgcggatcg ccgccgccca
     2281 tgaggccaag cgaggccctg aggccttccc aggcgggctt caggtcgatc atccccaggt
     2341 cctggaagag cctggcggcg ctcgcgtggg gcccggccga aatcctcgcg aagagccggt
     2401 cgcaggcgtc gaactccgcg acgagggccg agggctccca tcgcatggtc gtcgccagga
     2461 agaggacggc ctcggacttg cactccagcc accggtcgaa gaagtcccgc gactgggagc
     2521 gcagctcctc gatcctcccg aggatgctcc aggtaatggc gacatagacc acgaggacga
     2581 gggcgatgag ggcgatgaag acctgggcgg ctgtggattg cgcgggcgct gcctcgtgtt
     2641 gcatagtctg gcgatgaacc tcggatatgg actgctgcgg aagggtatca cggcagcttt
     2701 ggatcgcgcc agctcgaaac ggctcctcgt cgtggcggcc ctcgtcgtgg cgggaggctt
     2761 gggcaacgcg ctcatgagcc tcgcgaacat ggccctctcg agccccttct tcttcgattc
     2821 cgtcttcacc gcggtctgcg ccgcgctttt tgggcccctg gcgggggccc tctgcggcct
     2881 cgtctcgcac ctgttcatgg agcttttcca cggatggaac ggcctcttcc ttcccttcgc
     2941 cgtatgcaac atcgccaccg gcctcatcgt gggcctcttc gcgcgctcgg ggcgcctcgc
     3001 gaccctgacc ggggccatgc tctgcaccat cctcgtgacc ctcgggaact cggtgctggg
     3061 cgcccttgtg gccttctatc tcttcggcgg catcacaggg cacgcgagcg actacctcgt
     3121 gacgggcttc atcctcgtgg gccagaccct cctcgccgcc gccttctggg cgcggattcc
     3181 ggctaacctc atcgacaagg ggatcgcggt ggccctcgcc tatttcgcgg cgaggcgtgc
     3241 tctgggacag cgggccgagg cggcccggct aggccggtga cccggccctg ggccccgacc
     3301 cccgactggc cccctgggcg acggcgcgga tgccggcgtc gagggcgtcg cgcagggcct
     3361 ctgagcctgg cctcgcgagc atgaccacgt cctttgtccc gaggaccccg gtggcaggga
     3421 cgatcttccc gccgaagaac ttcctggaga ggtactcgcc caccttggcc tcggtggccg
     3481 cgccgtcgac ctcctcccag atcagcttga ccatcccgtc gaggtcgttc tcgatataga
     3541 cgagctcgca gcccgtcgag gctatcacct ggtccacata ggatgccttc tgcactgcga
     3601 tgcgcttgcc gcggagctcg aggctgccct ccgcggggag cttcgaggca tgcatgaaga
     3661 ccctcacctc gaaacgctcg tagggcatgg tggggatgac ggaggcttcg cggaaggcgc
     3721 tgttcggcca gccagcgttg atgacgaggt cgatgtcgcc attctcgaag gcgtcgcgga
     3781 tcctgtccca ttcgtcgccg acaatctcga tctcgaggcc ggccgccgcg ctgatcctcc
     3841 tgaggaggtc gacgctcacg ccagccgagt tgcccttgtc caggtagacc caaggaggat
     3901 aggtcacgtc gtgtcccacc ctgatccggc ggccctccgc gcggcggccg cccgaagtga
     3961 acgaggccga ggtcctcgag gcgtcgaggg ccagggccga gaggatctcc ttctcggagg
     4021 ccatgctcgc cacgatgtgg ctcgagctcg agttgaggaa acgcgtgtcg gtcgcgagcc
     4081 gggcgacccg tcccaggatg aggccggcgc tctccgactg ttccctcgag aggtcccgca
     4141 cgtgtgcgat gcgcgacgcc gcggctgaca gggcctccga cgagctgccg agtcgctcgg
     4201 agctgagccg cacgtcggcg atgatctcgc ccttgatggc cccgaacctc tccatggagc
     4261 tgcccgattc gtcgaggccc ttctggaggg cgatgaggcg ctgggacagc tcggccgtga
     4321 tgcgcttcgt ggtctcggcg aggttgcgga cctccttcgc caccacggcg aagcccttgc
     4381 cgtgctcgcc cgccctggcg gcctggatgg cggcgttcag ggcgaggagg ttggtgagct
     4441 cggagatgtc gacgatgcgg gcgatctgat cgaggatcgc cttggcctcg ccgcggatcg
     4501 tctggaacag gtcggcgatg ctcccgaagg tggcgtcgag cgagtccacg ctcgtcttcg
     4561 tcttgtccat ggcggccagg ctccgcatgg attcctcgcg ggagccgctg acggcctcgt
     4621 cggccgcctc gaggtcggag aggaccagcc gggagctgga gtcgatgcgg tccagctccc
     4681 ccttcacctc ggcgagggag cgctcgtgcg cgccgcccga gtcgaggacc ttcccgacga
     4741 agatggagtt gaggtcggag acctgtcgga ggtcgtggag gaagcccgag agccgcttct
     4801 ggaaatccgc gaagacgatg atcccatcga gggttagccc cgtcgaagcc ttgccaccgt
     4861 ccgtgcctga cccgctgccc tgcgccatgg gggattgtac gcgacgatct tcggaatcgt
     4921 caaaccaatc ctaacggggg gagtcgtgga tggccctagg ggtggtgcag gccggccttc
     4981 cgcctcgcct cctggaaggt gtcggcgtcc gagaggcg
//
LOCUS       DASSQV010000423         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_971205, whole genome shotgun sequence.
ACCESSION   DASSQV010000423 DASSQV010000000
VERSION     DASSQV010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971205"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFL04_07390"
     CDS             <1..467
                     /locus_tag="VFL04_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839565.1"
                     /translation="RLALYEPPFIVDSSRPPLPGDYFQRISALLAEGRRGEAVEYFLS
                     TGPAMPGELIARMRKTPMWAGLEALANTLPYDGLVMGDTMAGRSLPEGAWAGAKMPVL
                     VMAGGASPAWAANSVRALASVLPDARSLMLEGQTHGASPESLLPALKEFFLA"
     gene            complement(445..2076)
                     /locus_tag="VFL04_07395"
     CDS             complement(445..2076)
                     /locus_tag="VFL04_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET7839566.1"
                     /translation="MVPSSAEAESRAIAALIRLMGGCAELRDLAGEVVTYLRGWSGCE
                     AIGLRLHEGDDYPYFQTLGFPGPFVQRESRLCALGVNGELIRGPDGRPILECMCGNII
                     RGRTDPEQPFFTKGGSFWSNNTTELLATTGDEERQAHTRNFCNKSGYESVALVPIRHG
                     GEILGLLQFNDHRPGRFSAELIQVFERFGECVGIAISQMLATRRLREGQERFRSLFEG
                     SPDGILETAGDGRILSANPAAARILGMSQAAIVAGGYAAVFDMADPRVEAVFAKRRAE
                     GHSQGRLRMRRGGGELFEAEVESSSYHTRGGEARIWKIFRDLSEALAAEEALRRKDEA
                     LEALARKREWLVRDSYHRVKNNLTVVESLLDLETADLADKRDEVLFTEAKGRVHAMLL
                     IHESLHMSADLERLGSESYVRGLIAGLFAAYQGEEPSISLVSEVEDIGMDPDTALSCG
                     LILNELISNAFKHAFAGRAKGRIGVVLRREGGELLLSVEDDGVGMAGDLDLERAGSLG
                     LRIVQMKARELGGSISVERDGGTRFSLRFRPGRTP"
     gene            2209..2625
                     /locus_tag="VFL04_07400"
     CDS             2209..2625
                     /locus_tag="VFL04_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7839567.1"
                     /translation="MKSQLNPYLSFRDKAREAMEFYKAAFGGELKMSTFKEYGVSKDP
                     AEDGKIMHSVLEVGNGIVFMAADTPNSMEYRYGSAISMSLSGDEAEELTGYFNRLSVG
                     GKVGEPLKKAPWGDTFGMLTDKFGVVWMVNISAKKS"
     gene            complement(2706..>2999)
                     /locus_tag="VFL04_07405"
     CDS             complement(2706..>2999)
                     /locus_tag="VFL04_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7839568.1"
                     /translation="GNAGPHKIEGVGIGYTPPLWRPDVADELLAVGTAEAKAMTRRLA
                     LEEGLFAGTSSGANVVAAIELGKRLGPGARVATLLVDSGLKYLSTDLFARKEA"
BASE COUNT          457 a         1061 c          996 g          485 t
ORIGIN      
        1 cccggctcgc cctctacgag ccgcccttca tcgtggactc ctcgcgtccg cccctgcctg
       61 gggactattt ccagaggatc tcggccctcc tcgcagaggg ccgcaggggc gaggctgtgg
      121 agtacttcct gtcaacgggc ccggccatgc cgggcgagct catcgcgagg atgcgcaaga
      181 ccccgatgtg ggccggcctc gaggccctgg ccaacaccct gccctacgac ggcctggtca
      241 tgggagacac gatggcgggc aggagcctgc ccgagggggc ctgggctggc gcgaagatgc
      301 ccgtcctcgt catggccggc ggggcgagcc ccgcctgggc cgccaactcg gtgcgcgccc
      361 tcgcctcggt cctccccgat gcgcgcagcc tcatgctgga agggcagacc cacggcgcca
      421 gccccgagtc cctcctgccc gcccttaagg agttcttcct ggcctgaagc gcaggctgaa
      481 tctcgtgccc ccgtcccgct ccacgctgat gctcccgccg agctccctcg ccttcatctg
      541 gacgatcctg agcccgaggg agcccgccct ctccaggtcg aggtctccgg ccatgcccac
      601 cccgtcgtcc tcgaccgaca gcaggagctc cccgccctcc ctgcgcagga ccaccccgat
      661 ccttcccttg gccctgccag cgaaggcgtg cttgaaggcg ttcgagatca gctcgttcag
      721 gatgaggccg caggacaggg ccgtgtcggg gtccatgccg atgtcctcca cctcgctcac
      781 gagggaaatg gacggctcct cgccctggta ggcggcgaag agcccggcga tgaggcccct
      841 gacatagctc tcggagccga gcctctcgag gtcggccgac atgtggaggc tctcgtggat
      901 gagcagcatc gcgtggaccc tgcccttcgc ctcggtgaac aggacctcgt cccttttgtc
      961 ggctaggtcg gcggtctcga ggtcgaggag gctctcgacg acagtcaggt tgttcttgac
     1021 gcggtggtag gagtcgcgca cgagccattc ccgcttacgc gccagggcct cgagcgcctc
     1081 atccttcctg cgcagggcct cctctgcggc gagggcctcg ctcaggtcgc ggaagatctt
     1141 ccagatcctg gcctcgcccc cccgggtgtg gtagcttgag ctctccacct cggcctcaaa
     1201 gagctccccc ccaccccgcc gcatgcgcag cctgccctgg ctatgcccct cggcccggcg
     1261 cttggcgaag actgcctcca cccgcgggtc ggccatgtcg aagactgccg cgtagccccc
     1321 agcgacgatc gcggcctggc tcatcccgag gatccgtgcc gccgccgggt tggcggagag
     1381 gatccgcccg tcgccggccg tctcgaggat cccgtcaggg ctgccctcga agagcgatcg
     1441 gaaccgctcc tggccttccc tgagccgcct cgtcgccagc atctgggaga tcgcgatccc
     1501 gacgcactcg ccgaagcgct cgaagacctg gatgagctcg gcgctgaagc ggccgggcct
     1561 gtggtcgttg aactggagaa ggccgaggat ctccccgcca tggcggatcg gcacgagggc
     1621 caccgattcg tagcccgact tgttgcagaa gttccgcgtg tgggcctgcc tctcctcgtc
     1681 gcccgtcgtg gccagaagct cggtggtgtt gttcgaccag aagctgccgc ccttcgtgaa
     1741 gaagggctgc tcgggatcgg tgcgccccct gatgatgttg ccgcacatgc actcgaggat
     1801 gggcctgccg tcaggcccgc ggatcaactc cccgttcacc ccgagggcgc agagccggct
     1861 ctcccgctgc acgaagggcc ccgggaagcc gagggtctgg aaataggggt agtcgtcgcc
     1921 ctcgtggagc ctgaggccga tggcctcgca gcccgaccag ccgcggagat aggtgaccac
     1981 ctctccggcg aggtcacgga gctcggcgca gccccccatg agcctgatga gggcagcgat
     2041 cgcccgggat tcggcctcgg cgctggaggg gaccatccct ccagtatacc agagttttgg
     2101 cgactgacca ccgaaaccgg taaaccaacg gcgcgcccgc ccaccaaccg gggccggacg
     2161 gggttatctt tgtccagagt ccgggatgcc ggacgcaagg gaggccacat gaagagccag
     2221 ctcaatccgt atctgtcatt cagggacaag gcccgagagg ccatggagtt ctataaggcc
     2281 gccttcggcg gcgagctcaa gatgagcacc ttcaaggagt acggggtgtc caaggacccc
     2341 gccgaggacg gcaagatcat gcactccgtg cttgaggtcg ggaacggcat cgtcttcatg
     2401 gccgccgaca cccccaacag catggagtac cgctacggct cggccatcag catgtccctg
     2461 agcggggacg aggccgagga gctgacgggc tacttcaaca ggctctcggt cggcggcaag
     2521 gtgggtgagc cgctcaagaa ggcgccctgg ggggacacct tcggcatgct caccgacaag
     2581 ttcggcgtgg tctggatggt caacatctcg gccaagaagt cctaggcccg gtttggtggc
     2641 cgcgtggcgc ggggcccggc cgcggcgcgg ggcacggcct cgggcgcccg cgccgcggca
     2701 tacgctcagg cttccttgcg cgcgaagagg tcggtgctga gatacttgag gcccgagtcg
     2761 acgaggaggg tcgcgaccct cgccccgggt ccgaggcgct tgcccagctc aatcgccgcg
     2821 accacgtttg cccccgagga ggtgccggcg aagaggccct cctccagggc gaggcgcctg
     2881 gtcatggcct tggcctcggc cgtgcccacg gcgagcagct cgtcagcgac gtcgggcctc
     2941 cacaggggtg gcgtgtagcc gatgcccact ccctcgatct tgtggggtcc cgcattgcc
//
LOCUS       DASSQV010000424         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_100115, whole genome shotgun sequence.
ACCESSION   DASSQV010000424 DASSQV010000000
VERSION     DASSQV010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100115"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFL04_07410"
     CDS             <1..506
                     /locus_tag="VFL04_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7839569.1"
                     /translation="VVVTRELGPLFTAFIVIARSGTAIATELGGMVVAHEMEAYVSVG
                     VNPISYLAAPRFLGVVISMLVLTTYFNFFGLLGSFAVVQVIKPIGIEEYFRNLAEVLR
                     PGDLLFGLLKSFVFGTIISVVSIYRGFSVERASTEVPVAGIRAVGSSFMLCIFADIAL
                     TGMQYLG"
     gene            510..1448
                     /locus_tag="VFL04_07415"
     CDS             510..1448
                     /locus_tag="VFL04_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7839570.1"
                     /translation="MATPIIELRGAVAVRQDVEILRAASFALAEGRAAFFMGTAGSGK
                     SMLLKIAAGLVVPSSGEVFFRGKPLSRMSRLENQEFRRVTGYVFQDAALWANQSLYDN
                     LALPIRVHDSGASRAEVDRMVHRAAEIVGYSQELRARPAELSSGERRLIGLARALVLD
                     PELLFMDEPAANLDESAAERVTEIVGALKARGRSLLVVTSISKFAWKFADEVGVLNDG
                     ALIAYGPYADIAARTEGSLRGITGRLRPRQAAEAEAPAPGSGLLGAWAAALAEDPRAG
                     QDFERPRPRDEDGATLGDIINAIPDEESDADREDGK"
     gene            1445..2521
                     /locus_tag="VFL04_07420"
     CDS             1445..2521
                     /locus_tag="VFL04_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET7839571.1"
                     /translation="MRFRIRHADRIVGLFVLLAAAGLVAGIILLGVNQRWFAKNYAYT
                     TRFNSGAGVPPGTAIFMKGFQVGKIQRLKLTDDNMVDADFVIFDTYYRKATQNSVLEL
                     VTSPIGLGTQLLFHPGKSAAQLPEGSFVPLADSEQGRALIEEELVDIPPKDDTITRLL
                     ASVNPLLENLGKTVVTVNRTLTELNRAIAGQTSSPLGRIVSGTANTVEHVDQLVVGMK
                     GQTATLVDQAGALVESLTKVADNVEATTAAIRDPTGLVPKLLDAKGSIKTILDDKNAL
                     YDRILASVSEIQGALQNIQTVTASLSSEMPTIAVTIDETRTAIKQTQDVLEGLRNNPL
                     LRGGIPERREQQSLYQSMREGGFE"
     gene            2518..3543
                     /locus_tag="VFL04_07425"
     CDS             2518..3543
                     /locus_tag="VFL04_07425"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839572.1"
                     /translation="MRAHNLVILAAVCAVMAACSSAPKAKTEFFEVRNKAAEFAKLGD
                     GFMATAQYPEAAKYYDQALRANSSVDYLEGVSASHSSLGRVYLATGDLAAAETEFGSA
                     RAYALMATSPTAEAAAVTGQGELRYAKGEKEAALALFDQALALAAKDEPRLAIALHDA
                     AVAKNGLGRSAEAMADLERAAGINTRLKRWTELGANRYVMASILAAGGKVDQALSTAL
                     LALAADKAAENGRGIAGDLAACASLASRLGRNDEAWSYWLRSFDTAIATGDAAAARRA
                     LVSLVGLAGPVGKPEEGLRYEALLRKLDRAGAAGSAGAGAGASGPGAGAAKPGSAPVP
                     GPAPGAP"
BASE COUNT          590 a         1272 c         1186 g          535 t
ORIGIN      
        1 tcgtggtggt cacgcgagag ctcggcccgc tgttcaccgc cttcatcgtc atcgcgcgct
       61 cgggcacggc gattgccacc gagctcggcg gcatggtcgt cgcccacgaa atggaggcct
      121 atgtctcggt gggtgtgaac ccgatctcct acctcgcggc tccgcgtttc ctgggcgtgg
      181 tgatctcgat gctcgtcctc acgacctact tcaatttctt cggactcctc ggctccttcg
      241 ccgtcgtcca ggtgatcaag cccatcggga tcgaggagta cttccgcaac ctcgccgagg
      301 tcctcaggcc gggcgacctg cttttcggcc tcctaaagag cttcgtgttc ggcacgatca
      361 tctcggtggt ctcgatctac cgcggcttct cggtcgagcg ggcgagcacc gaggttccgg
      421 tcgcgggcat ccgcgccgtg ggatccagct tcatgctgtg catcttcgcc gacatcgccc
      481 tcacgggcat gcagtacttg ggctaggcca tggcgacgcc gatcatcgag ctccgcggag
      541 ccgtggccgt acgccaggac gtcgagatcc tgagggcggc ctccttcgcc ctcgccgagg
      601 gcagggccgc cttcttcatg gggacggcgg gctcgggcaa gtccatgctg ctcaagatcg
      661 ccgccggcct cgtcgtcccg agctccggcg aggtcttctt caggggcaag ccgctttcga
      721 ggatgtcgag gctggagaac caggagttcc gcagggtcac gggctacgtc ttccaggacg
      781 ccgcgctctg ggccaaccag agcctctacg acaacctcgc cctgccgatc cgggtgcacg
      841 acagcggcgc ctcgagggcc gaggtcgacc gcatggtcca ccgcgcggcc gagatagtcg
      901 gatacagcca ggagctcagg gccaggccgg ccgagctctc ctcgggggag cgccgcctca
      961 taggcttggc gcgggccctt gtcctcgacc ccgagctcct cttcatggac gagcccgctg
     1021 ccaacctcga cgagtcggcg gccgaacgcg tcacggagat agtgggggcc ctgaaggcca
     1081 ggggaaggag cctcctcgtg gtcacctcga tctcgaagtt cgcgtggaag ttcgccgacg
     1141 aggtcggcgt gctcaacgac ggcgccctca tcgcctacgg cccctacgcc gacatcgcgg
     1201 cccggaccga gggcagcctg cgcggcatca ccggcaggct caggccgagg caggctgccg
     1261 aggccgaggc gccggcgccg ggctcaggcc ttctgggcgc ctgggccgca gccctcgccg
     1321 aggatccccg cgcggggcag gacttcgagc ggccaaggcc ccgcgacgag gatggggcca
     1381 ccttgggcga catcatcaac gccattcccg atgaggaatc ggacgcggac cgggaggatg
     1441 ggaaatgagg ttcaggatca gacacgctga ccgcatcgtc ggactgttcg tgctcctggc
     1501 ggcggcgggg ctcgtggcgg ggatcatcct cctcggggtc aaccagcgct ggttcgcgaa
     1561 gaactacgcc tacacgacgc ggttcaactc cggggccggt gtcccgcctg gcacggcgat
     1621 tttcatgaag ggcttccagg tgggcaagat ccagcgcctc aagctcaccg acgacaacat
     1681 ggtggacgcc gacttcgtga tcttcgacac ctactacagg aaggcgacgc agaactccgt
     1741 cctcgagctg gtgacctccc ccatcggcct cggcacccag ctcctcttcc acccagggaa
     1801 aagcgccgcc caactccccg aggggagctt cgtccccctc gccgattcgg agcagggcag
     1861 ggccctcatc gaggaggagc tcgtcgacat cccgccgaag gacgacacga tcacgaggct
     1921 cctggccagc gtgaaccccc tgctcgagaa cctcgggaag actgtcgtga ccgtgaaccg
     1981 caccctcacc gaactcaacc gggcgatcgc cggccagacc tcgtcgcccc tggggcgcat
     2041 cgtctccggg accgccaaca cggtcgagca cgtcgaccag cttgtcgtcg gcatgaaggg
     2101 ccagaccgcg accctcgtgg accaggccgg ggccctcgtc gaatccctga ccaaggtggc
     2161 cgacaacgtc gaggcgacga cggccgccat ccgcgatccg acgggtctcg tgcccaaact
     2221 cctcgacgcg aagggctcga tcaagaccat cctcgacgac aagaacgccc tctacgacag
     2281 gatcctcgcg agcgtctctg agatacaggg cgcgctgcag aacatccaga cggtcaccgc
     2341 ctcgctgagc tccgagatgc ccaccattgc cgtgacgatc gacgagacga ggacggcgat
     2401 aaagcagacc caggacgtgc tcgaggggct caggaacaat cccctgctca gggggggcat
     2461 ccccgagcgc cgcgagcagc agtccctcta ccagagcatg agggaagggg gcttcgaatg
     2521 agggcgcaca acctggtcat tctggcggcg gtctgcgccg tcatggcggc ctgctcctcg
     2581 gcccccaagg ccaagacgga gttcttcgag gtcaggaaca aggccgccga gttcgcgaag
     2641 ctcggcgacg ggttcatggc cacggcccag tacccggagg ccgcgaagta ctacgaccag
     2701 gccctccgcg ccaactcctc ggtcgactac ctcgagggtg tgtcggcctc gcactcttcc
     2761 cttggccggg tctacctcgc gaccggagac ctcgcagccg ccgagaccga gttcggcagc
     2821 gcccgagcgt acgccctgat ggccacctcc ccgacggccg aggcggccgc ggtcacgggc
     2881 cagggcgagc ttcggtacgc gaagggggag aaggaggcgg ccctcgccct cttcgaccag
     2941 gccctggccc tggcggccaa ggacgagccc cggctcgcca ttgccctcca cgacgcggcc
     3001 gtcgcgaaga acggcctcgg ccgaagcgcc gaggcgatgg ccgacctcga gagggccgcg
     3061 gggatcaaca caaggctcaa acgctggacc gagctcgggg ccaaccgcta tgtcatggcc
     3121 tcgatcctgg ccgccggtgg caaggtcgac caagcgctct cgaccgccct gcttgccctc
     3181 gccgccgaca aggccgcgga gaacgggcga gggatagcgg gggacctggc ggcctgcgcc
     3241 agcctcgcct cccggctcgg ccgcaacgac gaggcctggt cctactggct gcgctccttc
     3301 gacaccgcca tcgccaccgg cgacgcagcg gcggcccgca gggcccttgt ctcccttgtt
     3361 ggccttgcgg gaccggtggg gaagcccgag gaaggtctga ggtacgaggc cctactgcgg
     3421 aaactcgacc gcgccggtgc ggcgggctcg gccggcgcgg gagccggcgc atccggcccg
     3481 ggagccgggg cggcgaagcc cggatcggca cctgtcccag gccccgctcc cggcgcgccg
     3541 tagcgatttc tgcctagctc ggagcctcga ccctgagggc cac
//
LOCUS       DASSQV010000425         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1218384, whole genome shotgun sequence.
ACCESSION   DASSQV010000425 DASSQV010000000
VERSION     DASSQV010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218384"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VFL04_07430"
     CDS             complement(<1..770)
                     /locus_tag="VFL04_07430"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7839573.1"
                     /translation="MRVIDSHVHFPEDRIIDPGTEPEGGAAAHGSAEAAGTGYTSGRK
                     GQGEGESHWLEAEKARWEAAWRLPAPVAVSKEEGERLWLEEFDGRDWLKAVVFVTAGS
                     NAFASELVARGEGRFQGYAHHDPLLPDAAARLERALLDGGLRGYKLLGPKIERRLDDR
                     SLDGIWEVAQEREAPVLIHFGIMGGAGGVANHMNINPLVIHDVAKRFPRMPIIVPHFG
                     CGYVFETLQLCWACPNVYIDTSGSNQWMRWVPYDLDLAT"
     gene            complement(774..1601)
                     /locus_tag="VFL04_07435"
     CDS             complement(774..1601)
                     /locus_tag="VFL04_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7839574.1"
                     /translation="MTKERRLAATTSYLALSLLAATMVFPFLWMLVSSLKDATQIFQS
                     ALFPAKPSLDNYALILLSKGTRFPAWFLNSLIVACGTTVSVLFFDSLVGYTLAKFSFP
                     GKKLIFLLVISTLMIPTEMLVIPWYALARALKWGNSYWGIMFPGMMSAFGVFLMRQFM
                     STIPDDLLDAARIDGRSELAIFLGVALPLVTPALATLAIFNFIGNWNAFLWPLIIASK
                     PAMFTLPVGLANFSGEAGSDWHLIMTGATVAMLPLIVVFLVFQKQIIKGIAMTGLKG"
     gene            complement(1612..2499)
                     /locus_tag="VFL04_07440"
     CDS             complement(1612..2499)
                     /locus_tag="VFL04_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7839575.1"
                     /translation="MSLARKRALTAYAFLALPLLFFVCVRFGPMLYMMGRSLTDWSLL
                     RKTGSFLGLANFQAVISDPSFAAAMGNTARYALIGAPAVIGLSLVIALLLNGIGRGRS
                     LFRLIYVLPYITPVVAVSWVWRLLMQTPPLGIINGMLTALGLPAHEFLNSPREALYSI
                     LAVNVWVEMGYCVTIFLAGIQTIPADCIEAARIDGASEAKVLTRIVLPLLVPITFFLA
                     IMEGIQFLRIFTPVYNMSAQAMGGPLDSTKSAALYIYQTAFTKFEMGRAAAASLILFA
                     VIMAVTLFQVRFFDRKTNY"
     gene            complement(2623..>3072)
                     /locus_tag="VFL04_07445"
     CDS             complement(2623..>3072)
                     /locus_tag="VFL04_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7839576.1"
                     /translation="GSFRIATLNAVKDLDWGVAELPTNQGIRSNFASFWTHGIVAGVT
                     GKKLEASVKFLKFITSPEVMEQWLAKVGELPANPKIGEKHKDDPYVSVFLKGLPYAHA
                     TAFVDEAGQRDLFVNMVDEINLKGAKTADVLHQVAVKEQKLIDDFWK"
BASE COUNT          544 a          894 c         1132 g          502 t
ORIGIN      
        1 gtcgcgaggt cgaggtcata gggcacccag cgcatccact ggttggagcc cgaggtgtcg
       61 atgtagacgt tggggcaggc ccagcagagc tggagggtct cgaagacgta gccgcagccg
      121 aagtggggga cgatgatggg catgcggggg aagcgtttcg cgacgtcgtg gatcacgagg
      181 gggtttatgt tcatgtggtt cgccaccccg cccgccccgc ccatgatccc gaagtggatg
      241 aggacggggg cctcgcgctc ctgggccacc tcccagatcc cgtcgaggga gcggtcgtcg
      301 agcctgcgct cgatcttggg gcccaggagc ttgtagcccc ggaggccgcc gtcgagcagg
      361 gcccgctcga gccgggcggc ggcgtcgggc aggagggggt cgtggtgggc gtagccctgg
      421 aaccttccct cgcccctggc cacgagctcc gaggcgaagg cgttgctgcc cgcggtgacg
      481 aagaccacgg ccttgagcca gtcgcggccg tcgaactcct cgagccagag ccgctcgccc
      541 tcctccttgg agaccgccac gggggccgga agccgccagg cggcctccca gcgggccttc
      601 tcggcctcga gccagtggct ctcgccttcg ccctggccct tccggccgct cgtgtagccg
      661 gtgcccgcgg cctcggctga gccgtgggct gccgcgccgc cctcgggctc ggtcccgggg
      721 tcgatgatcc tgtcctcggg gaagtggacg tgggagtcta tgacgcgcat gctctatcct
      781 ttcaagcctg tcatcgctat gcccttgatg atctgcttct ggaagacgag gaagaccacg
      841 atgagcggca gcatcgcgac ggtggccccc gtcatgatga ggtgccagtc gctccccgcc
      901 tcgcccgaga aattggccag gcccacgggc agagtgaaca tcgcgggctt ggacgcgatg
      961 atcaggggcc agaggaaggc gttccagttg ccgatgaaat tgaagatcgc gagggtcgcg
     1021 agggccgggg tcacgagggg cagggccacg ccgaggaaga tcgcgagctc gctccggccg
     1081 tcgatcctcg cggcgtcgag gaggtcgtcg gggatcgtcg acatgaactg gcgcatgagg
     1141 aagaccccga aggcgctcat catgccgggg aacatgatcc cccagtagga gttgccccac
     1201 ttgagggccc gcgcgagggc gtaccagggg atcacgagca tctccgtggg gatcatgagg
     1261 gtgctgatga cgaggaggaa gatgagcttc ttccccggga agctgaactt ggccagggtg
     1321 tagcccacga gggagtcgaa gaagagcacc gagaccgtgg tgccgcaggc cacgatcagg
     1381 ctgttgagga accaggcggg gaagcgcgtg cccttggaca ggaggatgag ggcgtagttg
     1441 tcgaggctgg gcttcgcggg gaaaagtgcg ctctggaaga tctgggtagc gtccttgagc
     1501 gaggagacga gcatccagag gaaggggaag accatcgtgg cggcgaggag gctcaaggcg
     1561 aggtaggagg tggtggcggc taacctgcgt tcctttgtca tgcccttggt cctagtagtt
     1621 ggtcttccgg tcgaagaacc tgacctggaa gagggtgacc gccatgatca ccgcgaagag
     1681 gatcaggctg gcggccgccg ccctgcccat ctcgaacttg gtgaaggcgg tctggtagat
     1741 gtaaagggcc gccgacttcg tcgagtccag gggtccgccc atcgcctggg ccgacatgtt
     1801 gtacaccggg gtgaagatgc ggaggaactg gatgccctcc atgatcgcga ggaagaaggt
     1861 gatcggcacg aggagcggca ggacgatccg cgtgaggacc ttggcctccg aggccccgtc
     1921 gatcctcgcc gcctcgatgc agtcggcggg gatggtctgg atgcccgcga ggaagatggt
     1981 cacgcagtag cccatctcca cccagacgtt gaccgcgagg atcgagtaga gggcctcgcg
     2041 cgggctgttg aggaactcgt gggcgggaag gccaagggcc gtgagcatgc cgttgatgat
     2101 gccgagcggc ggggtctgca taaggaggcg ccagacccag ctcacagcga cgacgggcgt
     2161 gatgtagggc aggacgtaga tgaggcggaa gaggctcctg ccccggccga tcccgttcag
     2221 gaggagggcg atcacgaggc tcaggccgat gacggcggga gccccgatga gggcgtagcg
     2281 cgcggtgttg cccatggccg ccgcgaagga tgggtcggag atcacggcct ggaaattggc
     2341 gaggccgagg aaggagccgg tcttgcgcag aaggctccag tccgtgaggc tcctgcccat
     2401 catgtagagc atgggcccga agcgcacgca gacgaagaag aggaggggca gggcgaggaa
     2461 ggcataggcc gtcagcgcgc gctttctggc aagggacatc aaggctcctg aaatgaggaa
     2521 gaaaccgcag agacgcagag gcgcagaggg gatatacgcg ccctgcgcca gctcgggcgc
     2581 tgctgcgcct ctgcagtgct ccctctcaac tgttcctcat tatcacttcc agaagtcgtc
     2641 gatgagcttc tgctccttga cggcgacctg gtggaggacg tccgcggtct tggcgccctt
     2701 gaggttgatc tcgtcgacca tgttgacgaa gaggtcgcgc tggccggcct cgtccacgaa
     2761 ggccgtcgcg tgggcgtagg gcaggccctt gaggaagacg ctgacgtagg ggtcgtcctt
     2821 gtgcttctcg ccgatcttcg ggttggcggg gagctccccg accttggcga gccactgctc
     2881 catgacctcg ggcgaggtga tgaacttgag gaacttcacc gaggcctcga gcttcttgcc
     2941 cgtgaccccg gccacgatgc cgtgggtcca gaaggaggcg aagttggacc tgatgccctg
     3001 gttcgtcggc agctcggcca cgccccagtc caggtccttc accgcgttga gggtggctat
     3061 gcggaaggag cc
//
LOCUS       DASSQV010000426         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2215324, whole genome shotgun sequence.
ACCESSION   DASSQV010000426 DASSQV010000000
VERSION     DASSQV010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2215324"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFL04_07450"
     CDS             complement(<1..225)
                     /locus_tag="VFL04_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HET7839577.1"
                     /translation="MTTYRLRNIDCPACAAGLEQGLRRVEGVRDVSIDFGTLSMRIDA
                     ADLAAVEAAVLRLEPLVSLTRIGAEAPGARP"
     gene            complement(222..626)
                     /locus_tag="VFL04_07455"
     CDS             complement(222..626)
                     /locus_tag="VFL04_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7839578.1"
                     /translation="MSSIAADKPDPALCSCSVLHEDRISRAKADTPSAPLVAELGSLF
                     KVFADETRLKILSALAAEELCVCDLSSVLGASQSAVSHQLAVLRAARLVRHRREGKVV
                     FYSLGDDHVGRILAIGLEHAGERFAQGAEAQR"
     gene            complement(730..1452)
                     /locus_tag="VFL04_07460"
     CDS             complement(730..1452)
                     /locus_tag="VFL04_07460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7839579.1"
                     /translation="MKAPERRSDEDPIQAEAEKILEARKLGADELWSAYERIAHGYAH
                     LSRTFMKTVRISDQYQMRLRELNAALADASRVDYLTGLLNRRAFFEGIEPERSRARRH
                     GRTLCVLMADIDDFKLVNDSHGHDAGDLALKGVARLLDAVLRKEDMKVRWGGEEFLAI
                     LPETGIGGGAATAEKLRALVEANAFPTHGAPIRLSISVGIALDDGGDIDAVISRADQA
                     LLEAKRAGKNRVMVSSAASSQP"
     gene            complement(1449..1835)
                     /locus_tag="VFL04_07465"
     CDS             complement(1449..1835)
                     /locus_tag="VFL04_07465"
                     /inference="COORDINATES: protein motif:HMM:NF020903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HET7839580.1"
                     /translation="MKDLLIAATKSSPSVRLESATGIAELAGESYPENAFEFYAPILA
                     WIRDFISAGEARFRLEIRLSYLNTSSTKCLIDLLDELEAAHRAGHRVEVAWHCDADNE
                     RCVESAEEFKEDVSLPFDILLDGAAE"
     gene            complement(1848..>2074)
                     /locus_tag="VFL04_07470"
     CDS             complement(1848..>2074)
                     /locus_tag="VFL04_07470"
                     /inference="COORDINATES: protein motif:HMM:NF039711.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6272 family protein"
                     /protein_id="HET7839581.1"
                     /translation="EDIGALTGRLEELRGMDKEALKGFYKRRLREPRTPGKGAGLGLI
                     DVARRSAAPLDFSIRELGDRFGFLSLRAIV"
BASE COUNT          321 a          687 c          716 g          350 t
ORIGIN      
        1 cggtctcgcg cccggcgcct cggcgccgat ccgggtgagt gacaccaggg gctcgaggcg
       61 gaggacggcg gcctcgacgg ccgcaaggtc ggcagcgtct atcctcatgg agagggtgcc
      121 gaaatcgatc gacacgtccc gcaccccctc gaccctgcga agaccctgct cgagccccgc
      181 ggcgcaggcg gggcagtcta tgttgcggag gcggtaggtc gtcacctctg agcctccgcc
      241 ccttgggcga agcgctcgcc ggcgtgctca agcccgatcg ccaggatcct gcccacatgg
      301 tcatcgccga gggaatagaa gacgaccttg ccttcgcgcc ggtgccggac gagcctggcg
      361 gccctgagca cggccagctg atggctcacc gctgattggc tcgcccccag gaccgacgag
      421 aggtcgcaga cgcagagctc ctcggccgcg agggccgaga ggatcttgag cctcgtctcg
      481 tcggcgaaga ccttgaagag gctcccgagc tcggcgacga ggggggccga gggcgtgtcc
      541 gccttggccc ttgagatcct gtcttcatgg aggacggagc aggagcagag ggcaggatcg
      601 ggcttgtccg cagcgatcga ggacatgcat acctccggga cgcatgaaca tatgaacaaa
      661 tagacatata attcatcgac ccgaggccgt caagcccagg aggggaggca agccgggggg
      721 gaccccggct cagggctggc tggaggctgc ggaggagacc atgacccggt tcttgcccgc
      781 ccgtttcgcc tcgaggaggg cctggtcggc ccggctgatg acggcatcga tgtctccgcc
      841 gtcgtcaagg gcgatgccga cgctgatgct gagccgtatg ggggcgccgt gggtggggaa
      901 ggcgttcgcc tcgacgaggg cccggagctt ctccgccgtc gccgctcctc ctccgatccc
      961 ggtctcgggg aggatggcga ggaactcctc gcctccccac ctcaccttca tgtcctcctt
     1021 cctgaggacg gcatcgagga gcctcgccac tcccttgagg gcgaggtcac cggcgtcgtg
     1081 gccgtggctg tcgttcacga gcttgaagtc gtcgatgtcg gccatgagca cgcagagggt
     1141 ccggccatgg cggcgggcgc gagagcgctc gggctcgatg ccctcgaaga aggcgcggcg
     1201 gttcaggagc cccgtgagat agtcgaccct ggaggcgtcg gcgagggcgg cgttgagctc
     1261 cctgagcctc atctggtact ggtcgctgat cctcaccgtc ttcatgaagg tcctgctgag
     1321 gtgggcgtag ccatgggcga tgcgctcgta ggcgctccac agctcgtcag cgccgagctt
     1381 cctggcctcg aggatcttct cggcctcggc ctggatgggg tcctcgtcgg acctgcgctc
     1441 cggggccttc attcggccgc tccgtcgagg aggatgtcga agggcaggct cacgtcctcc
     1501 ttgaactcct cggccgactc gacgcaacgc tcgttgtcag cgtcgcagtg ccaggccacc
     1561 tcgacccgat gtccggcgcg atgcgcggcc tcgagctcgt cgagcaggtc gatgaggcat
     1621 ttcgtgctgc tcgtgttgag gtagctcagc ctgatctcga gcctgaaccg ggcctccccc
     1681 gccgagatga agtcgcggat ccaggcgagg atgggggcgt agaactcgaa ggcgttctcg
     1741 ggataggact cgccggcgag ctcggcgatg ccagttgccg attcgaggcg gaccgaggga
     1801 gatgacttgg tcgcggcgat caagaggtct ttcatccctc ccccctttca gacgatggcc
     1861 ctgaggctga ggaacccgaa gcggtcgccc agctcgcgga tcgagaagtc caggggcgcg
     1921 gctgaacgcc tcgcgacgtc gatgaggccc aggccggccc ccttccccgg cgttctcggc
     1981 tcccgaagcc ggcgcttata gaagcccttc agcgcctcct tgtccatccc ccggagctcc
     2041 tcgagcctcc ccgtcagggc cccgatgtcc tccc
//
LOCUS       DASSQV010000427         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_847048, whole genome shotgun sequence.
ACCESSION   DASSQV010000427 DASSQV010000000
VERSION     DASSQV010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847048"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFL04_07475"
     CDS             <1..497
                     /locus_tag="VFL04_07475"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HET7839582.1"
                     /translation="VWLSVPFNLCLAALAAVFLFSLVSWALLDRKAEVLLFFPDAKTS
                     RIRGEVRDLPRAWGAEARAELVASEVLLGPRDSSLAPAFPAGTRVEAVLYRKGRLYVD
                     ISPEAAISEPSPLKLGLEALRRSLAVAVPQAARIALTIGGIEPYAEGIDEEPGNTAKN
                     RKIN"
     gene            576..1655
                     /locus_tag="VFL04_07480"
     CDS             576..1655
                     /locus_tag="VFL04_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HET7839583.1"
                     /translation="MRRNSIIILIALLFVGALAVSADEAVIMDFSLLKADILPDQANQ
                     GKFLENRATMMDFSGTAGASYTAEQKKQMRVSLAIRNWDVVLASSSRNTTTTQLSQTA
                     EATLTANAPQFAGKTVLGVRVRFPLEDFNSWAKVQPPYEIPAFEPKATVDDQGNITPA
                     AAQAGTDPVNASLTRFEGSYDANTKIITALGIVKNVGVIKSVAVTVKGLNFPHGLTLV
                     LKDADGNEKSMFMGYLNFDGWKELRWENPAYVSDVRNRELRIYPLYPKTVPMVKLDGF
                     VFTRDADSVGGDFVTYIKDVKILYDKAVLEPVRDIDDEAIWGIVSKKEDDRKISESKR
                     FGNQQVLRYIEKLKQESKTDFTPTK"
     gene            1778..3043
                     /locus_tag="VFL04_07485"
     CDS             1778..3043
                     /locus_tag="VFL04_07485"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7839584.1"
                     /translation="MPGDNAEAIPDRGELRSSYDSRRQGYARVLNIMERKIRSSLEAA
                     SLRPTIKGRIKSFDSWYAKRIRLLRQAKALGREPIPITDVIALKAVCPFLGDLSRAER
                     TICADFQVVEIERKGSERSFREFGYESIHMLVELPCELAKSVPGLDAPVIEVQLRTIL
                     QEAWAEVEHELVYKAEFTPFDEPMKRKLAALNANLSLSDIIFQEILDYQRRLNFELER
                     RRSSFHGKIEEAMDGPLQLEEEGLIDLPGGPEPAPSIGPEIDAGESASMDDLLLAALS
                     AHNRENFERAVRIYSEILAQSPGKEIAAVVYKHRGMAHFSQSRYEEAIADFGASLELD
                     PGCYKAAYYRGVVRCVLEDFQAALGDFDLALSIHPYHFYSRFRRGMAWWHLGDYAQAL
                     ADSESALKLEPNNEQAGRLRALALAKLKM"
BASE COUNT          604 a         1079 c          937 g          533 t
ORIGIN      
        1 gggtctggct ctccgtcccc ttcaacctct gcctggccgc gctggccgcg gtgttccttt
       61 tctccctggt ctcctgggcc ctcctggacc gcaaggccga ggtcctcctc ttcttccccg
      121 acgcgaagac gagccgcatc aggggcgagg tgcgcgatct tccccgtgcc tggggcgccg
      181 aggcgagggc cgagcttgtg gcctccgagg tcctcctcgg gccgcgggac tcgagtctgg
      241 cgccggcctt ccccgcgggc acgagggtgg aggccgtcct ctacagaaag ggccggctct
      301 atgtcgacat ctcccccgag gcagccatct ccgagccctc gcccctcaag ctcggcctgg
      361 aggccctgcg ccgcagcctg gccgtggccg tgcctcaggc ggcgcgcatc gcgttgacca
      421 tagggggcat cgagccctac gccgagggca tcgacgagga gcccggaaat accgcgaaaa
      481 atcggaaaat taattgacaa agattcctcc gggcatatac ttctctatca aatagcggaa
      541 aatgggataa cgctcacaat gaaaggagtt actgaatgag acggaactca attatcatcc
      601 tgattgcgct tctattcgtg ggcgcgctcg cggtttcggc cgacgaggca gtcatcatgg
      661 atttctccct gctcaaggca gacatccttc ccgaccaggc caaccagggc aagttcctcg
      721 agaaccgcgc cacgatgatg gatttctcgg gaacggccgg cgccagctac accgctgagc
      781 agaagaagca gatgcgcgtc tcgcttgcga tccgcaactg ggacgtcgtt ctcgcctcct
      841 cctcgcgcaa cacgaccacg acccagctct cgcagaccgc cgaggccacg ctcacggcca
      901 atgccccgca gttcgccggc aagaccgtcc tgggcgtgcg cgtgcgcttc cccctcgagg
      961 atttcaacag ctgggccaag gtccagcctc cctatgagat ccccgccttc gagcccaagg
     1021 ccaccgtgga cgaccagggc aacatcaccc cggccgcggc ccaggcgggc accgatccgg
     1081 tcaacgccag cctcacccgc ttcgagggca gctacgacgc caacaccaag atcatcaccg
     1141 ccctcggcat cgtgaagaac gtgggcgtca tcaagtccgt ggcggtcacg gtcaagggcc
     1201 tgaacttccc ccacggcctc accctcgtcc tcaaggacgc cgacggcaac gagaagtcca
     1261 tgttcatggg ctacctgaac ttcgacggct ggaaggaact ccgctgggag aaccccgcct
     1321 acgtgagcga tgtccgcaac cgcgagctca ggatctaccc cctctacccg aagaccgtcc
     1381 ccatggtcaa gcttgacggc ttcgtcttca cccgcgatgc cgacagcgtg ggcggcgact
     1441 tcgtcaccta catcaaggac gtcaagatcc tctacgacaa ggccgtcctc gagcctgtaa
     1501 gggacatcga cgacgaggcc atctggggca tcgtgtccaa gaaggaagac gaccgcaaga
     1561 tcagcgagtc caagcgcttc ggaaaccagc aggtcctccg ctacatcgag aagctgaagc
     1621 aggagagcaa gaccgacttc accccgacca agtaatccgg tctgggacgg gacacgcgga
     1681 gccgggaccc ggtctgcgcc atggcattgg cgcaaacggg gttccggttc cgcttttttg
     1741 ttggccatcc ttggcgattg gggtgtagat tatggccatg ccgggagaca atgccgaggc
     1801 aatacctgac cggggcgagc tgcgttcatc ctacgattcg cgcagacagg gctacgccag
     1861 ggtcctcaac atcatggagc gcaagatcag gtcctccctc gaggccgcct cgctccggcc
     1921 caccataaag gggcggatca agtccttcga ctcctggtac gcgaagcgga tcaggctgct
     1981 ccgccaggcc aaggccctgg gacgggagcc gatcccgatc accgatgtca tcgccctcaa
     2041 ggccgtctgc cccttcctgg gggacctgtc cagggccgaa cgcacgatct gcgccgactt
     2101 ccaggtggtg gagatcgaaa ggaagggctc ggagcgctca ttccgagagt tcggctacga
     2161 gtccatacac atgctcgtcg agctgccctg cgagctcgcg aagtcggtgc ccggccttga
     2221 cgccccggtc atcgaggtgc agctcaggac catcctgcag gaggcctggg ccgaggtgga
     2281 gcacgagctc gtctacaagg ccgagttcac ccccttcgac gagcccatga agcgcaagct
     2341 ggccgctctg aacgccaacc tgagcctttc cgacatcatt ttccaggaga tactcgatta
     2401 ccagcgcagg ctcaacttcg agctcgagcg ccggcgctcg agcttccacg gcaagataga
     2461 ggaggcgatg gacggccccc tccagctcga ggaggagggc ctgatcgacc tcccgggagg
     2521 cccggagccg gcgccctcca tcggacccga gatcgatgct ggggagtcgg caagcatgga
     2581 cgacctcctc ctcgcggccc tgagcgccca caaccgcgag aacttcgaaa gggccgtgcg
     2641 gatctacagc gagatactgg cccagagccc cggcaaggag atcgccgccg tcgtgtacaa
     2701 gcacaggggc atggcgcatt tctcccagtc gcgctacgag gaggcaatcg ccgatttcgg
     2761 cgccagcctg gagctcgatc caggctgcta caaggcagcc tactaccgcg gcgtggtcag
     2821 atgcgtgctc gaggatttcc aggccgcgtt gggcgacttc gacctcgccc tctcgatcca
     2881 cccctaccac ttctactcgc ggttccgtcg cggcatggcc tggtggcacc tcggcgacta
     2941 tgcgcaggcc ctcgccgaca gcgagtccgc cctgaagctc gagccgaaca acgagcaggc
     3001 cggacgcctc agggccctgg ccctcgcgaa gctcaagatg tgaggctcga atgctgccgt
     3061 ccttgacact gcggctttcc ctccgatata ttccgttctc gcacgtgtcc ccatcgtcta
     3121 gaggttagga cagcgggttt tcatcccgcc aac
//
LOCUS       DASSQV010000428         3545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_100674, whole genome shotgun sequence.
ACCESSION   DASSQV010000428 DASSQV010000000
VERSION     DASSQV010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100674"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1708
                     /locus_tag="VFL04_07490"
     CDS             113..1708
                     /locus_tag="VFL04_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7839585.1"
                     /translation="MTTRSVTRLALAVALSLTCTAAFGAGGGDYLRIALLREPATADA
                     QLTADSYCVPLNIYDRLVESQTTKTGFSQLVPGLAEKWDVSADGLVYTFYLRRGVRFS
                     DGEVLKADDVLFTFDRMLDPATKAINTDFLDMIAGAAERMGGRASSVSGLQVLDDHTI
                     RIRLAKPFGPFVANLASPACSIYERRATVAAGSRFGLDPARTVGTGAFTLESWTADGR
                     LRLKAKPDYFRGKPSVAGVEFRVVPDAGAQRRLFEAGELDILDLDYAQTQAPYFLGSG
                     TWKNFIASGPRGGIHYLALNEAIKPLGDPRIRKALQLGIDRKAILDGIYGGRGSLENG
                     IFPRGLIAHNPELPAIPFDPGRARALIVEAGYPNGFDLELAAVRDGGPTLRICEALQA
                     MLAELGLRVRITQMDPAAYLAARKEGRLPSYEAEWSADYNDPDNFIYTFFSEKNTAAR
                     SCNYRNKAVFARIEEARTMADQARRLKVYQALERTVVQDDAAWVPLFSLDHLFVLQPR
                     VKNFKVSWNGWSDMPYYGVSLSQ"
     gene            1785..2507
                     /locus_tag="VFL04_07495"
     CDS             1785..2507
                     /locus_tag="VFL04_07495"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET7839586.1"
                     /translation="MKALIGITTNLEQRPGCRYHTLTERYVRSVIRAGGLPIILPSWS
                     DPSSPADYLDRIDGLLLTGGGDIAPILYGEDPLAGLKSVSQRRDQAELALAKEALARG
                     MPLFGVCRGHQVLNVAMGGSLYQDIATQLPGSSKHDAGEFPYEEPWHLIRLQGRGTRL
                     FEIFGKESVGTNSFHHQAVKELAPGLRATATSADGVIEAMEAEEGSSFVLSVQFHPEG
                     MIDGDPGFLGLFKAFVRAASAG"
     gene            complement(2496..>3545)
                     /locus_tag="VFL04_07500"
     CDS             complement(2496..>3545)
                     /locus_tag="VFL04_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET7839587.1"
                     /translation="ELGIPSERRGTAVVGLLRGAKEGPTVALRADIDALPVKEEVETP
                     FRSRNEGLMHACGHDAHVAIQLGAARLLGVMREDLAGNAKFLFQPAEEGDGGADTMVK
                     DGCLESPRVDRVYGLHVMPDLRVGSVELKKGALCGSSATLSITVGGKGSHGAYPHLGT
                     DAILIASNVVMSLNVLVSRFVSPLEEAVLSIGTIRGGTRSNIVADRVEMEATLRTTSA
                     AVQEILIDRATSIVEGVAASFGGRGELRVKQGYAALVNHDEAVDEIEAVARSLVGEER
                     IRWKEKPSMGVEDFSFFIAERTGAFYHLGCGNAEEGITAPLHSPLFRIDEACLPLGAA
                     MQAGLVLSFLEKSAR"
BASE COUNT          573 a         1254 c         1173 g          545 t
ORIGIN      
        1 gggagcacgt tgacatcgag ttgggcgatg gtatagtggc tcatccctta actcagacgc
       61 gcgtttccga agccagtgcc gagaccggtc cagcgggacc gaaaggggaa gcatgacgac
      121 aaggtctgtg acgaggctcg ccctcgctgt ggccctgtcc ctcacctgca ccgcggcctt
      181 cggcgccggg ggcggtgact acctccggat cgccctgctc agggagcccg ccacggccga
      241 cgcccagctg accgcggaca gctactgcgt ccccctcaac atctacgacc gcctcgtcga
      301 gtcccagacc accaagaccg gcttctccca gctcgtgccg gggctcgccg agaagtggga
      361 tgtctccgcc gatggccttg tttacacctt ctacctccgc aggggggtga ggttctccga
      421 cggcgaggta ctcaaggccg atgacgtcct cttcaccttc gaccggatgc tggacccggc
      481 gacgaaggcc atcaacaccg atttcctcga catgatagcc ggggccgccg agcggatggg
      541 cggcagggcc tcctccgtga gcgggctcca ggtcctcgac gaccacacga tccgcatcag
      601 gctcgccaag cccttcgggc ccttcgtggc caacctcgcg tctcccgcct gctcgatcta
      661 cgagcggagg gccacggtcg cggcgggaag ccgcttcggc ctcgacccgg ccaggaccgt
      721 cggcacgggg gccttcaccc tcgagtcctg gacggccgac ggcaggctga ggctcaaggc
      781 caagcccgac tacttccggg gcaagccctc ggtcgctggg gtcgagttca gggtcgtccc
      841 cgacgcgggg gcccagcgcc gcctcttcga agccggggag ctcgacatcc tggacctcga
      901 ctacgcgcag acccaggccc cctacttcct cggcagtggc acatggaaga acttcatagc
      961 gagcgggccg agggggggga tccactacct cgccctcaac gaggccataa agcccctcgg
     1021 cgaccccagg atcaggaagg ccctccagct gggcatagac agaaaggcca tcctcgacgg
     1081 gatctacggg gggaggggct cgctcgagaa cgggatcttc ccgaggggac tcatcgcgca
     1141 caacccggag ctccccgcga tccccttcga ccccgggagg gccagggccc tcatcgttga
     1201 ggccggctac ccgaatggct tcgacctgga gctcgccgcg gtcagggacg gcggcccgac
     1261 gctcaggatc tgcgaggccc tgcaggccat gctcgccgag ctcgggctca gggtgaggat
     1321 cacccagatg gatccggcgg cctacctcgc cgcccgcaag gagggcaggc tcccctccta
     1381 cgaggcagag tggtcggccg actacaacga tcccgacaac ttcatctaca ccttcttctc
     1441 ggagaagaac accgctgccc ggtcctgcaa ctaccggaac aaggcggtct tcgcgaggat
     1501 cgaggaggcg aggacgatgg ccgaccaggc caggcggctg aaggtctacc aggccctcga
     1561 gaggacggtc gtccaggacg acgcggcctg ggtgcccctg ttctcgctcg accacctctt
     1621 cgtcctccag cccagggtca agaacttcaa ggtctcgtgg aacgggtgga gcgacatgcc
     1681 ctactacggg gtaagcctgt cccaataggc ggggcttccc ggcgccgctt caccacccgg
     1741 gcgtattcgt ttattatcgc ccgggtccac gaggaggctc ccccatgaag gccctgattg
     1801 gcatcaccac gaaccttgag cagaggcccg gctgccgcta ccataccctg accgagcgct
     1861 atgtccgctc cgtgatcagg gcggggggcc tgcccatcat cctgccctcc tggtccgacc
     1921 cctcaagtcc cgccgattac ctggatagga tcgacggact cctcctcacc gggggcggcg
     1981 acatcgcccc catcctctac ggcgaggatc ccctggccgg cctcaagtcg gtgtcccagc
     2041 gccgggacca ggccgagctc gccctcgcga aggaagccct cgcgcggggc atgcccctgt
     2101 tcggcgtctg ccggggccac caggtcctca acgtggccat ggggggcagc ctctaccagg
     2161 acatcgccac ccagctgccc ggctcatcga agcacgacgc cggcgagttc ccctacgagg
     2221 agccctggca cctcatcagg cttcagggcc gcgggacgag gctcttcgag atctttggga
     2281 aggagagcgt cgggaccaac tccttccatc accaggcggt aaaggagctc gccccggggc
     2341 tcagggccac ggccacgagc gccgacgggg tcatcgaggc gatggaggcc gaggaaggct
     2401 cgagcttcgt cctctcggtc cagttccacc ccgagggcat gatcgacggc gatccaggct
     2461 tcctcgggct cttcaaggcc ttcgtgcggg cggcctcagc gggctgattt ctccaggaag
     2521 gagagcacca ggccggcctg catggccgcg ccgaggggga ggcaggcctc gtcgatgcgg
     2581 aacagcgggc tgtggagggg ggcggtgatc ccctcctccg cgttgccgca gccgaggtgg
     2641 taaaaggccc cggtccgctc cgcgatgaag aaggagaagt cctcgacccc catgctcggc
     2701 ttctccttcc agcggatgcg ctcctcgccg acgaggctcc gggccacggc ctcgatctcg
     2761 tctacggcct cgtcgtggtt cacgagggcc gcatagccct gtttcacccg cagctccccc
     2821 ctgccgccga aggacgcggc caccccctcg acgatcgagg tggcgcggtc gatgaggatc
     2881 tcctgcacgg cagcgctcgt ggtcctgagg gtcgcctcca tctccacccg gtcggcgacg
     2941 atgttcgagc gcgtgccccc cctgatggtc ccgatggaga ggacggcctc ctcgaggggc
     3001 gagacgaagc ggctcacgag gacgttgagc gacatgacga cgttggacgc gatgaggatg
     3061 gcgtcggtcc cgagatgggg ataggccccg tgcgagccct tgcccccgac cgtgatggag
     3121 agggtcgcgc tcgagccgca gagggcgccc ttcttgagct cgacgctgcc caccctgagg
     3181 tcgggcatga cgtggaggcc gtagacccgg tccacgcggg gggactcgag acagccgtcc
     3241 ttcaccatcg tgtcggcgcc cccgtcgccc tcctcggcgg gctggaagag gaacttcgcg
     3301 ttgccggcga ggtcctcgcg catgacgccc aggagcctgg ccgccccgag ctggatcgcg
     3361 acgtgggcgt cgtggccgca ggcgtgcatg aggccctcgt tgcgcgagcg gaagggcgtc
     3421 tccacctcct ccttgaccgg cagggcgtcg atgtcggcgc ggagggccac ggtgggcccc
     3481 tccttcgcgc cgcgcaggag gccgacgacg gccgtgcccc tgcgctcgct cgggatgccg
     3541 agctc
//
LOCUS       DASSQV010000429         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_847157, whole genome shotgun sequence.
ACCESSION   DASSQV010000429 DASSQV010000000
VERSION     DASSQV010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847157"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFL04_07505"
     CDS             complement(<1..626)
                     /locus_tag="VFL04_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839588.1"
                     /translation="MIQMSCIAGLVKSIELADREFGRMDEGLRALMRNADELVAVACG
                     STALGLAAAARDLPRLPLRVIADPSGDGLEPLPGARGSIVLGLSFSGASVEVGDLVGR
                     AADAGIETVYAGPPAACPAKAHCLRLDLGGLPRRYLPLAACLLIHRVWGPGPDARLVD
                     ALRATASVGPSGLSGFLEAAFRSPRIPVFVSAGPGLRAALLAAQYLEFL"
     gene            742..2538
                     /locus_tag="VFL04_07510"
     CDS             742..2538
                     /locus_tag="VFL04_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839589.1"
                     /translation="MSDSNQNRKEALENEAQNYIRLGTLMRRARPDDRAEAVAQVLAD
                     SVPIEEKIRRIERIDSVPISRGPRKPAAPRPPSAPGAEPQRVKQTVAAAARRRAHLKA
                     EVAPSSFMQYFFREGMEIRKRAKGNGLLKAGLFSVALEEAATREFLDRAKKELVPPLI
                     SALGTTLREGWRFLRKSEYNRLAALNRLVTELNNLDLQHISPHEPGSAKRMFPLESAF
                     LYFRVDPSIPAEAVMSLDEVLAKLSHPRHESEEAYSSTRRLLQNGGPPACLQDFVLAF
                     NMVRTRRYLSVSDLMRVEPGPLVSLNEFDCSEGIQDKIDSHIASLLERLDKIGEENEK
                     VTRLRTFVTRDKDGNIDYGPLAAAYEAGGEAGPVWKKDEDNAILVATGLAERLVGLVV
                     PLLGNAGAAAEAARRALPLPGAPAVNAPAIQPATRIEDKALLYRISLMQNVATKLRKM
                     LATLPNLPQVRFLAIKARAVQATKFDADGAALIGEAADLFYMIASRLADLLLSSDLFP
                     VESPDSDPPAPAKEVPLPSLDNIALRQSLSTAATLAYLAALRFQEPTMDAALKNDRQA
                     DDQRRDILGEIERLSDADTFQEARRKAGLHHS"
BASE COUNT          464 a          963 c          868 g          336 t
ORIGIN      
        1 aggaactcaa ggtactgggc cgcgaggagg gccgcccgga gccctgggcc ggcggagacg
       61 aagacgggga tgcgcggact ccgaaaggcg gcctcgagaa agccggaaag gccggacggt
      121 ccgacgctgg ctgtggcgcg caacgcgtca acgaggcggg cgtctgggcc cggcccccag
      181 accctgtgga tgaggaggca ggccgcgagg ggcaggtagc gccggggcag gcctccaagg
      241 tcgaggcgca gacagtgggc cttggcgggg caggccgcgg gaggtccggc gtagaccgtc
      301 tcgatccccg cgtcggcggc gcggccgacc aggtccccga cctcgaccga ggcgcccgag
      361 aagctgagcc cgagcacaat ggagccccgg gccccgggca ggggctcgag cccatccccc
      421 gagggatcgg cgatgacccg aaggggaagg cgtggcaggt ccctcgccgc ggcggccagg
      481 ccgagggctg tcgagccgca ggcgacggcc acaagctcat cggcattccg catcagggcg
      541 cggagccctt cgtccatcct cccgaactcg cggtcggcga gttcgatgga cttcacgagg
      601 cccgctatgc agctcatctg gatcaaggta acaaagccga ggcccccgcg ggtacgggcc
      661 gggagtccgt ccccgccgag gccgccgttg cgcccgcgga gacaaatcaa tatgctatac
      721 aaataagggg cggggcgggc gatgtccgat tcgaaccaga acaggaagga agccctcgag
      781 aacgaggcgc agaactacat ccgtctcggc accctcatgc ggagagcccg gcccgacgac
      841 cgggccgagg ccgtggccca ggtcctggcg gactccgtgc ccatcgagga gaagatccgc
      901 aggatcgaga ggatcgactc ggtcccgatc tcgagggggc cgcgcaagcc ggccgccccc
      961 cggcctccct cggcacctgg agccgagccc cagcgggtca agcagaccgt ggccgcggcc
     1021 gcccgccgcc gggcccacct gaaggccgag gtcgccccct cgagcttcat gcagtacttc
     1081 ttccgggagg gcatggagat caggaaacgg gcgaagggca acggcctcct caaggccggg
     1141 ctcttctccg tcgcgctcga ggaggccgcg acgagggagt tcctggaccg ggcgaagaag
     1201 gagctcgtcc cgcccctcat ctcggccctc ggcacgacgc tgcgcgaggg ctggcgcttc
     1261 ctcaggaagt cggagtacaa ccgcctggcc gcgctgaacc gcctcgtgac cgaactcaac
     1321 aacctcgacc tccagcacat atcgccccac gagccgggga gcgcaaaacg catgttcccc
     1381 ctggagtcgg ccttcctgta cttccgcgtc gatccctcga tcccggccga ggccgtcatg
     1441 tccctcgacg aggtgctcgc gaagctctcc cacccacggc acgagagcga ggaggcctat
     1501 tcctccaccc gcaggcttct gcagaacggc ggccccccgg cctgcctcca ggatttcgtc
     1561 ctcgccttca acatggtgag gaccaggcgc tacctctcgg tctccgacct catgagggtg
     1621 gagcccggac ccctcgtgag cctcaacgag ttcgactgca gcgagggcat ccaggacaag
     1681 atcgactccc acatagcctc gctgctcgag cgcttggaca agatcggcga ggagaacgag
     1741 aaggtcacga ggctcaggac cttcgtgacc cgggacaagg atggcaacat cgactacggc
     1801 cccctcgccg ccgcctacga ggccgggggc gaagccggcc ccgtgtggaa gaaggacgag
     1861 gacaacgcca tcctggtcgc gacgggcctc gccgagcgcc tggtcggcct cgtggtcccc
     1921 ctcctcggca acgccggggc cgcggccgag gcggcgaggc gggccctgcc cctgccggga
     1981 gccccggcgg tgaacgcgcc cgccatccag cccgccactc ggatcgagga caaggccctg
     2041 ctctaccgga tatccctcat gcagaacgtc gccacgaagc tcaggaagat gctggcgacc
     2101 ctgcccaacc tgccccaggt ccgcttcctc gccatcaagg cccgagccgt ccaggccacc
     2161 aagttcgacg ccgacggggc ggccctcata ggcgaggcgg ccgacctctt ctacatgatc
     2221 gcgagcaggt tagccgacct cctgctttcc agcgacctct tccccgtcga gagcccggac
     2281 tcagaccccc cggcccccgc gaaggaggtc cccctgccct ccctggacaa catcgcgctc
     2341 cgccagtccc tctccacggc agcgaccctc gcctatctcg cggccctccg cttccaggag
     2401 cccacgatgg acgccgccct caagaatgac cggcaggccg acgaccagcg ccgcgacatc
     2461 ctgggcgaga tcgagcgcct ctcggacgcc gacaccttcc aggaggcgag gcggaaggcc
     2521 ggcctgcacc actcctaggg ccatccacgc gtcccaccaa ggccccgcgg ctgctcctcg
     2581 tgcagcgatg ccgcgttagg ttttatttga cgactccgag gatcgtcgca t
//
LOCUS       DASSQV010000430         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2340157, whole genome shotgun sequence.
ACCESSION   DASSQV010000430 DASSQV010000000
VERSION     DASSQV010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340157"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1406
                     /gene="gdhA"
                     /locus_tag="VFL04_07515"
     CDS             60..1406
                     /gene="gdhA"
                     /locus_tag="VFL04_07515"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET7839590.1"
                     /translation="MNSYSAQVLEELRTRYPWETEFLQAATEVLESISPLIDAEPRYR
                     EQSILERIVEPERSVIFRVPWMDDTGHYRVNRGYRVQFNSAIGPYKGGLRFHPKVSLS
                     TLKFLGFEQMFKNSLTGLPMGGGKGGSDFDPNGKSDNEIMRFCQSFMTELVKHVGPDT
                     DVPAGDIGVGGREVGFLFGQYKRIANEFSGVLTGKGINFGGSLVRPEATGFGAIYFAE
                     EMLKLKNDSLKGKTVSVSGFGNVAWGACIKATQLGARVVTISGPDGYVYDPDGIGTQE
                     KWDYLTQMLVIDRNKVETYVRKFPNAKFFAGKKPWEQKVDIAMPCAIQNELDLEDAKA
                     LLANGVKVVVETSNMGCTADAWKLFVARGIPFAPGKAANAGGVATSGLEMSQNSMRLL
                     WGAEEVDKRLHEIMVNIHKTCVETAKKYGFEGNYVVGANIAGFKKVADAMIDQGLV"
     gene            1585..2169
                     /locus_tag="VFL04_07520"
     CDS             1585..2169
                     /locus_tag="VFL04_07520"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HET7839591.1"
                     /translation="MKYGVVHQASYSRGQLLLRTIFGWLYIAIPHGFLLFFVAIWGAV
                     LGFLAFWAILFTGKYPAAWFDYQVKLQRWAARLNSRLMNLTDAKAAFGLGGTSEAVTV
                     EAERPERISRGLTLLRLILGILYVVIPHGFCLFFRLIASSVLSFLAWWAVLFSGHYPE
                     RWHAFNVGTYRWIFRVGLYMSNMTDAYPPFSGKE"
     gene            2177..>2717
                     /locus_tag="VFL04_07525"
     CDS             2177..>2717
                     /locus_tag="VFL04_07525"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET7839592.1"
                     /translation="MSAPADETPYGGGGSPAGGPSETGGAELRPSGLVLPRPDEVPQR
                     DRDDAMGAYLMMFASWAVGLPFPFLNLIASWVYYLVNRRGSRFVAFNSYQALLSHLPV
                     AFYNALIVVWLLVALFGGHTLSSNFLPAAIIAAVLNLAYVVFSVIALRRATKGIFYYI
                     PWFGRIAFGHFYGPRALARE"
BASE COUNT          452 a          990 c          791 g          484 t
ORIGIN      
        1 cgcggcgcgg gcgacgccag ccgccagcag aacagtgaag catcataagg agcaacagca
       61 tgaactcgta cagcgcgcag gtccttgagg aactcaggac ccgctatcct tgggagacgg
      121 agttcctcca ggccgcgacc gaggtcctcg agtcgatctc gcccctgatc gacgccgagc
      181 cgaggtaccg cgaacagagc atcctggagc gcatcgtcga acccgagcgc tcggtcatct
      241 tccgcgtgcc ctggatggac gacacgggcc actaccgcgt caaccgcggc taccgggtcc
      301 agttcaacag cgccatcggt ccctacaagg gtggcctgcg cttccacccc aaggtctccc
      361 tctccaccct gaagttcctc ggcttcgagc agatgttcaa gaactccctc accggcctcc
      421 ccatgggcgg cggcaagggc ggctcagact tcgaccccaa cggcaagagc gacaacgaga
      481 tcatgcgctt ctgccagagc ttcatgaccg agctcgtcaa gcacgtcggt cccgacacag
      541 acgtccccgc cggcgacatc ggcgtgggcg gccgcgaggt cggctttctc ttcggccagt
      601 acaagcgcat cgccaacgag ttctccggcg tcctcaccgg caagggcatc aacttcggcg
      661 gctccctcgt caggcccgag gccacgggct tcggcgcgat ctacttcgcc gaggagatgc
      721 tcaagctcaa gaacgacagc ctcaagggca agaccgtctc ggtctcgggc ttcggcaacg
      781 tggcctgggg cgcctgcatc aaggccaccc agctcggcgc cagggtcgtc accatctccg
      841 gccccgacgg ctacgtctat gaccccgacg ggatcgggac ccaggagaag tgggactacc
      901 tcacccagat gctcgtgatc gaccggaaca aggtcgagac ctacgtccgc aagttcccga
      961 acgccaagtt cttcgccggc aagaagccct gggagcagaa ggtcgacatc gcgatgccct
     1021 gcgccatcca gaacgagctc gacctcgagg acgcgaaggc cctcctggcc aacggggtca
     1081 aggtcgtggt cgagacctcg aacatgggct gcacggccga tgcctggaag ctcttcgtcg
     1141 cgcgcggcat ccccttcgcc cccggcaagg ccgccaacgc gggcggagtg gccacctcgg
     1201 gcctcgagat gagccagaac tccatgcgcc tgctctgggg cgccgaggag gtcgacaagc
     1261 ggctccacga gatcatggtg aacatccaca agacctgcgt cgagaccgcc aagaagtacg
     1321 gcttcgaggg caactacgtc gtcggcgcga acatcgcggg cttcaagaag gtggccgacg
     1381 cgatgatcga ccagggcctc gtgtaacgct ttcctgatcc gcggaaccgg acagacggtt
     1441 tccgcccgtc ccgatggccg ccccgatggg ggcggccatt ccttttccgc tgccctccgg
     1501 cgccggccgc tttgcgaatt tgcactgaga acgaatattc actaccatca ctgtattctc
     1561 tcatgcatct cacaggaggt cgtgatgaag tacggcgttg tccaccaggc ttcgtactct
     1621 cggggccagc ttctcctcag gaccatcttc ggatggctct acatcgccat cccgcatggc
     1681 tttctcctct tctttgtggc gatctggggc gcggtcctgg gcttccttgc cttctgggcc
     1741 atcctcttca ccggaaaata ccccgccgcc tggttcgact accaggtcaa gctgcagagg
     1801 tgggccgcca ggctcaactc ccggctcatg aacctcaccg acgccaaggc ggcctttggc
     1861 ctgggcggga cgagcgaggc cgtcaccgtc gaggccgaga ggcccgagcg gatctcgagg
     1921 ggcctcaccc tgctgcgcct catcctcggc atcctctacg tcgtgatccc gcacggcttc
     1981 tgcctcttct tccgcctcat cgccagctcg gtcctgtcct tcctcgcctg gtgggccgtg
     2041 ctcttcagcg gccactatcc cgagcgctgg cacgccttca acgtgggcac ctaccgctgg
     2101 atcttccgcg tgggcctgta catgtcgaac atgaccgacg cgtatccgcc cttcagcgga
     2161 aaggagtgac gggacgatga gcgcccccgc cgacgaaacc ccgtatggcg ggggcggatc
     2221 ccccgcgggc ggcccctccg agaccggggg ggccgagctt cggccttccg gcctcgtcct
     2281 gccccggccc gacgaggtcc cccagcgcga ccgcgacgac gcgatggggg cctacctcat
     2341 gatgttcgcg tcctgggccg tgggcctgcc ctttcccttc ctcaacctca tcgcctcctg
     2401 ggtctactac ctcgtcaacc gccgcggctc gcgtttcgtc gccttcaact cctaccaggc
     2461 cctcctctcc cacctgcccg tggccttcta caacgccctc atcgtggtct ggctcctcgt
     2521 ggccctgttc ggcggccaca ccctctcgtc caacttcctg cccgcggcca tcatcgcggc
     2581 cgtccttaac ctcgcctatg tcgtcttctc ggtgatcgcc ctgaggcggg ccaccaaggg
     2641 catcttctac tacatcccct ggttcggccg catcgccttc ggccacttct acgggccgcg
     2701 ggccctcgcc cgtgagg
//
LOCUS       DASSQV010000431         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1468015, whole genome shotgun sequence.
ACCESSION   DASSQV010000431 DASSQV010000000
VERSION     DASSQV010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468015"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFL04_07530"
     CDS             <1..911
                     /locus_tag="VFL04_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839593.1"
                     /translation="DLIPKRGLSTRTIVAIAVAGLAVLGLVLGLSLGGRRDGTVESAG
                     AHRAPVEYRLDKSPFEKRLYLGDSLLVNSGTDTYKLLISNIGDAVTIDTPAGPTRLML
                     GEEGTIDLDKNNQPELKVLVTDLSKKDSSKGALLHFEFSSAEAALKASSEAPTPDSPA
                     TAPAAASAPAAAPAATAPAPAAPSPVSVPAGSKSVVLFESGKSPYPFVVSVTFRGSCM
                     FRYEVDRKDRDERYYRKGETISVSANNSVKLWASNGQSAKLSVQASGGKNADIDLGGP
                     GEVSVKRIAWSQTDSGGWTLSALDVD"
     gene            922..2283
                     /gene="rimO"
                     /locus_tag="VFL04_07535"
     CDS             922..2283
                     /gene="rimO"
                     /locus_tag="VFL04_07535"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET7839594.1"
                     /translation="MRFWIDRHGCAKNQVDGEEMLSRLEAAGHLYVDSPEGAELIIVN
                     TCGFIEDAKKESIDAVIGLKAAHPKAKVLVAGCLAERYSEALSSELPEADGIVGNADL
                     GAIIEAAEETAAGHRPVLLPHAAPSLGTVTRKRLFDFPGTAHVKITEGCSNNCSYCAI
                     PLIRGSLRSRPVAEVVAECSELVARGIGELVLIGQDLGSYGRDSGSSRLPELLSALSA
                     LEGDFRVRVLYIHPDNFPEAILPVMAADPRLLAYFDLPFQHASAPILKAMHRRGDAAA
                     YLGLIARIRSALPEAVLRSTVLVGFPGETEEDFRRLLEFQAAARLDWLGSFAFSREEG
                     TAAFDLKGRVPKKVVAERRRRVEEAQEGITRERLERFVGQSLEVIVEEPVETDPDGDE
                     DGEPLSIGRAWLQAPEVDGLTVLRGRFEPGARVLAKVLGVRGVDLEAMPLQPADSTAP
                     RRG"
     gene            2294..>3090
                     /locus_tag="VFL04_07540"
     CDS             2294..>3090
                     /locus_tag="VFL04_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7839595.1"
                     /translation="MPEKSRGLAPYLSSLNPEQLAAVRHEGRSLLILAGAGSGKTRVI
                     TTKIAWLVRERGLAPESILAVTFTNKAAREMRDRAASLEPSCSRSVIRTFHSFGAWFL
                     RRNAQAAGLDPNFVIYDDDDATTLLHGILPQNSRSECSRMAQAIARAKDYGLEPDSPD
                     LSAAFSDPAMRKVYALYEERLRKTGNVDFGDLIRLPAKLLRDDETVRRRTRQRFSVIL
                     VDEYQDSNVAQFELLRLLAGHGHDPSDRGGAYLCVVGDDDQSIYRFRG"
BASE COUNT          487 a         1202 c          964 g          437 t
ORIGIN      
        1 acgacctgat ccccaagcgg ggcctctcca cgaggaccat cgtcgccatc gccgtggccg
       61 gcctggccgt gctgggcctg gtcctgggcc tgagcctcgg cggcaggagg gacgggaccg
      121 tcgagagcgc cggcgcgcat cgcgcccccg tggagtaccg cctcgacaag agccccttcg
      181 agaagcggct ctacctcggc gattccctcc tcgtgaactc gggaaccgac acctacaagc
      241 tcctgatctc gaacatcggc gacgccgtca ccatagacac ccccgccggc cccacccgcc
      301 tcatgctcgg cgaggaaggc accatcgacc tcgacaagaa caaccagccc gagctgaagg
      361 tcctcgtgac cgacctgtcg aaaaaggaca gctcgaaggg cgccctgctc cacttcgagt
      421 tctcgagcgc cgaggccgcc ctcaaggcct cctcggaggc tccgaccccc gacagcccgg
      481 ctaccgcccc ggccgcggct tccgccccgg cggcggcccc cgccgcaacc gcccccgccc
      541 cggccgcgcc gagccccgtg tcggtgcccg ccggcagcaa gagcgtcgtc ctctttgagt
      601 cgggcaagag cccctacccc ttcgtggtct cggtgacctt ccgcggctcc tgcatgttcc
      661 gctacgaggt cgaccgcaag gaccgcgatg agcgctacta ccgcaagggc gagaccatca
      721 gcgttagcgc caacaactcc gtgaagctct gggcctccaa cggccagtcg gccaagctca
      781 gcgtccaggc ctcgggcggc aagaacgccg acatcgacct cggcggcccg ggcgaggtct
      841 cggtcaagcg catagcctgg tcccagaccg actcgggcgg ctggaccctg agcgccctcg
      901 acgtggacta gcagagggcc gatgcgtttc tggatagaca ggcacggctg cgcgaagaac
      961 caggtcgacg gcgaggagat gctctcgcgg cttgaggccg cgggccacct ctacgtcgac
     1021 tcccccgagg gggccgagct catcatcgtc aacacctgcg gcttcatcga ggacgccaag
     1081 aaggagtcga tcgacgccgt catcggcctc aaggcggccc atcccaaggc caaggtcctc
     1141 gtcgccggct gtctcgccga gcgctactcc gaggccctgt cgtccgagct ccccgaggcc
     1201 gacgggatag tcggcaacgc cgacctcggc gccatcatcg aggccgccga ggagacggcg
     1261 gccggccaca ggcctgtcct cctgccccac gccgccccct cgctcgggac cgtcacccgc
     1321 aagaggctct tcgacttccc cgggaccgcc cacgtcaaga tcaccgaagg ctgctccaac
     1381 aactgcagct actgcgccat ccccctcatc aggggctcgc tccggtcgcg gccggtggcc
     1441 gaggtcgtgg ccgagtgctc ggagctcgtg gccaggggaa tcggcgagct ggtcctcatc
     1501 ggccaggacc tgggctccta tggccgggac tcgggctcca gccgcctccc cgagctcctt
     1561 tccgccctct cggccctcga gggggacttc cgagtccgcg tcctctacat ccaccccgac
     1621 aacttccccg aggccatcct cccggtgatg gccgcggacc cccgcctcct cgcctatttc
     1681 gacctgccct tccagcacgc atcggcgccg atcctcaagg ccatgcaccg ccgcggggac
     1741 gctgctgcct acctcggcct catagcccgg atccgctccg ccctgcccga ggctgtcctc
     1801 cgctcaaccg tcctcgtggg cttccccggc gagaccgagg aggacttccg ccgcctcctc
     1861 gagttccagg ccgccgccag gctggactgg ctcggcagct tcgccttctc gcgcgaggag
     1921 ggcacggccg ccttcgacct gaagggccgc gtgcccaaga aagtcgtggc cgagcgccgc
     1981 agacgggtcg aggaggccca ggagggcata acgcgggagc ggctggagcg cttcgtgggc
     2041 cagagcctcg aggtcatcgt cgaggagccc gtcgagaccg accccgacgg ggacgaggac
     2101 ggggagcccc tgtccatcgg ccgagcctgg ctccaggccc ccgaggtcga cggcctcacc
     2161 gtgctccgcg ggcgcttcga gcccggcgcc agggtcctgg ccaaggtcct cggggtgagg
     2221 ggggtcgatc tcgaggccat gccccttcag cccgccgact ccacagcgcc gcggcgcggc
     2281 taggccaggc agcatgcccg agaagagccg gggactcgcg ccctacctct cttccctcaa
     2341 ccccgaacag ctggccgccg tccgccacga gggcaggagc ctcctcatcc tcgcgggggc
     2401 gggctcgggc aagaccaggg tcatcaccac gaagatagcc tggctcgtgc gcgagcgggg
     2461 cctggccccc gagtcgatcc tggccgtgac cttcaccaac aaggccgcgc gcgagatgcg
     2521 cgacagggcg gcctcgctcg agccctcctg ctcccgctcc gtcatcagga ccttccactc
     2581 cttcggggcc tggttcctca ggaggaacgc ccaggccgcc ggcctcgacc ccaacttcgt
     2641 gatctacgac gacgacgacg cgacgaccct cctccacggc atcctgcccc agaacagccg
     2701 ctccgagtgc tcgaggatgg cccaggccat agccagggcc aaggactacg ggctcgagcc
     2761 cgactcgccc gacctttcgg cggccttctc cgatccggcg atgcgcaagg tctacgccct
     2821 ctacgaggag cggctccgga aaacgggcaa cgtcgacttc ggcgacctca tccgcctgcc
     2881 cgcgaagctg ctccgggacg acgagaccgt ccgacgccgc acgaggcagc gcttttccgt
     2941 catcctggtc gacgagtacc aggactcgaa cgtcgcccag ttcgagctcc tgcgcctcct
     3001 cgccggtcac gggcacgatc catcagaccg cggcggcgcc tacctctgcg tcgtcgggga
     3061 cgacgaccag tcgatctatc gtttccgcgg
//
LOCUS       DASSQV010000432         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1468153, whole genome shotgun sequence.
ACCESSION   DASSQV010000432 DASSQV010000000
VERSION     DASSQV010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468153"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="VFL04_07545"
     CDS             complement(<1..1291)
                     /locus_tag="VFL04_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HET7839596.1"
                     /translation="MELKLDLANLASAFPSDLTPDQRAKAQTLFLKRLSATAHEFFGG
                     KMQTVPKCGLWGFNWFNVWYTPGVSAVSTAIRDENDRSFALSNRGNLVAVVSDSTRVL
                     GDGDCTPPGGLGVMEGKAFLMKYLGGVDGVALCIDSRGKDGKHDPDRIIEFVKMVAPS
                     FGAVNLEDISQPNCFKVLDELREACDIPVWHDDAQGTACVTLAGLINALKLAGKKIGD
                     SRIVLLGAGAANTTIARLIIADGGDPAKIVLFDSKGSLHAGRSDIERNKSDYRKWELC
                     TTTNPKRYASEAEAIKGADVLIALSTPGPDTVKREWVASMAAKSIVFACANPVPEIYP
                     HAAKEAGAFIVATGRGDFPNQVNNSICFPGLLKGALLVRARKITDGMAIRCAHSIADF
                     SEKRGINPDDIIAKMSETEIFAIQAADVAMKAIEEGVA"
     gene            complement(1421..2452)
                     /locus_tag="VFL04_07550"
     CDS             complement(1421..2452)
                     /locus_tag="VFL04_07550"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET7839597.1"
                     /translation="MAKVKVGVAGYGVIGQRLADGVALQGDMELVGVADVAPTLSVRA
                     LKERGMPYALYCAADEKRADLEKAGVPVSGSMEDLVRKVDVMLDATSAGVGAKNKELY
                     AKHGKKGIFQGGEKNSVADVFFHGYANYEKGLDKQFLKLTSCNTTGLIRAVDCADRVA
                     GVEKVVITIIRRVADPGDYHRGLTNALQIDHAPSHQALDLMTIMPHIDATGILVHTPV
                     THGHIITIVMTTKRELSVEAALEAFRAHPRIRVVTIAEGFQGNASFFRYARDLGNPRG
                     DMYEIGLWEDSIVKSGRDLMFAINIPQESVTIPETMDGIRACMGMQRDRLEGTAMTNK
                     YLGIGKWLG"
     gene            complement(2501..>3160)
                     /gene="iolE"
                     /locus_tag="VFL04_07555"
     CDS             complement(2501..>3160)
                     /gene="iolE"
                     /locus_tag="VFL04_07555"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HET7839598.1"
                     /translation="TTKPYAEVEKAFIAHRDFLYAVGARVIGAAEQGHSIQGQDKAVF
                     AAKPSFSDEEWKRLCEGLNKLGVLAKEKGMSLTYHHHMGTGVQTAAEIDRLMEGTKAG
                     LLDLLYDTGHLVFSGEDHLAVLRKWGKRIRHVHLKDVRPAVVERVRKEGLSFLTAVKA
                     GAFTVPGDGSIDFVPVFAELKALGYTGWWVVEAEQDPAKANPLEYAIKARKYIRDKAG
                     I"
BASE COUNT          445 a         1022 c         1096 g          597 t
ORIGIN      
        1 gggccacgcc ctcctcgatg gccttcatcg cgacgtcggc cgcctggatg gcgaagatct
       61 cggtttcgct catcttggcg atgatgtcgt cgggattgat gccgcgcttc tcggagaagt
      121 cggcgatcga gtgggcgcag cggatggcca tgccgtcggt gatcttgcgc gccctcacga
      181 ggagggcccc cttgaggagg ccggggaagc agatcgagtt gttcacctgg ttggggaagt
      241 cgccgcggcc cgtggccacg atgaaggccc ccgcctcctt ggcggcgtgg gggtagatct
      301 cgggcacggg gttggcgcag gcgaagacga tggacttcgc cgccatcgag gccacccact
      361 cgcgcttgac cgtgtcgggc ccgggggtcg acagggcgat gaggacgtcg gcgcccttga
      421 tagcctcggc ctcgctcgcg taacgcttgg ggttcgtggt ggtgcagagc tcccatttgc
      481 ggtagtcgct cttgttgcgc tcgatgtcgg agcgccccgc gtggagcgag cccttggagt
      541 cgaagaggac gatcttcgcg gggtctccgc cgtcggcgat gatgagccgg gcgatggtgg
      601 tgttggccgc gccggcgccg aggaggacga tcctggagtc gcctatcttc ttgcccgcga
      661 gcttgagggc gttgatgagg ccggcgaggg tgacgcaggc cgtgccctgg gcgtcgtcgt
      721 gccagacggg gatgtcgcag gcctcgcgca gctcgtccag gaccttgaag cagttgggct
      781 gggagatgtc ctcgaggttg accgcgccga aggagggggc gaccatcttc acgaactcga
      841 tgatcctgtc ggggtcgtgt ttgccgtcct tgccccggga gtcgatgcag agggccaccc
      901 cgtcgacgcc gccgaggtac ttcatgagga aggccttgcc ctccatgacc cccaggccgc
      961 cgggaggggt gcagtcgccg tcgccgagga cccgtgtcga gtcggacacg acggccacga
     1021 ggttgccgcg gttggagagg gcgaaggagc ggtcgttttc gtcgcggatc gcggtggaga
     1081 cggcggagac gccgggggtg taccagacgt tgaaccagtt gaagccccag agcccgcact
     1141 tgggcacggt ctgcatcttg ccgccgaaga actcgtgggc agtggccgag agcctcttga
     1201 ggaaaagggt ctgggccttc gcgcgctggt caggcgtcag gtccgatggg aaggccgatg
     1261 cgaggttggc gaggtcgagc ttgagttcca tgttcccttc cttatgctgc ccatgatgtt
     1321 cgttgccgcg tgattgtaac caagccccga gggcctgtat agctggggag aaacccgggc
     1381 cgcggcggcc ggcccgggct ccccagctgg ccggcccgct ctagcccagc cacttcccga
     1441 tgccgagata cttgttggtc atggccgtcc cttcgaggcg gtccctctgc atccccatgc
     1501 aggccctgat cccgtccatc gtctccggga tcgtgaccga ctcctgcggg atgttgatcg
     1561 cgaacatgag gtccctgccg gacttcacga tcgagtcctc ccacaggccg atctcgtaca
     1621 tgtcgccgcg ggggttcccg aggtcgcggg cgtagcggaa gaagctcgcg ttgccctgga
     1681 agccctcggc gatggtcacc accctgatgc gcgggtgggc cctgaaggcc tcgagggcgg
     1741 cctcgaccga cagctcccgc ttcgtcgtca tcacgatcgt gatgatgtgg ccgtgggtca
     1801 ccggggtgtg caccaggatc cccgtcgcgt cgatgtgggg catgatcgtc atgaggtcga
     1861 gggcctggtg gctcggcgcg tggtcgatct gcagggcgtt ggtcaggccc cggtggtagt
     1921 ccccagggtc ggccacccgc ctgatgatcg tgatcaccac cttctccacc cccgccaccc
     1981 ggtcggcgca gtccacggcc ctgatcaggc ctgtcgtgtt gcaggaggtg agcttgagga
     2041 actgtttgtc caggcccttc tcgtagttcg cgtagccgtg gaagaagacg tcggcgaccg
     2101 agttcttctc gccgccctgg aagatgccct tcttgccgtg cttggcgtag agctccttgt
     2161 tcttggcccc aacacccgcg ctcgtcgcgt ccagcatgac gtcgaccttc ctgacgaggt
     2221 cctccatgct cccggacacg gggacgccgg ccttctcgag gtcggcgcgc ttctcgtcgg
     2281 ccgcgcagta gagggcgtag ggcatgcccc gctccttgag ggcgcgcacc gagagggtcg
     2341 gcgccacgtc ggccacgccc acgagctcca tgtcgccctg cagggccacc ccgtcggcga
     2401 gcctctgccc gatgacgccg tagccggcca ctccaacctt cacctttgcc atatcgatgc
     2461 ctcctgagca ctcttcaaga gaatggaacc gcagcgcgcc ctagatgccc gccttgtcgc
     2521 ggatgtactt cctggccttg atcgcgtact cgagggggtt ggccttcgcc gggtcctgct
     2581 cggcctcgac cacccaccag cccgtgtagc ccagggcctt gagctcggcg aagacgggga
     2641 cgaaatcgat ggagccgtcg ccgggcaccg tgaaggcccc ggccttgacc gcggtgagga
     2701 agctcagccc ctccttcctc acccgctcga ccacggcggg cctcacgtcc ttgaggtgga
     2761 cgtgcctgat ccgcttgccc catttcctga ggacggcgag gtggtcctcg cccgagaaga
     2821 cgaggtggcc cgtgtcgtag agcaggtcga ggaggccggc cttcgtgccc tccatgagcc
     2881 tgtcgatctc cgcggccgtc tgcacgccgg tgcccatgtg gtggtggtag gtgaggctca
     2941 tgcccttctc cttggccagc actcccagct tgttgaggcc ctcgcagagc ctcttccact
     3001 cctcgtcgct gaagctcggc ttggcggcga agacggcctt gtcctggccc tggatcgagt
     3061 ggccctgctc ggccgcgccg atcacgcggg cgcccacggc gtaaaggaag tcgcggtggg
     3121 cgatgaaggc cttctcgacc tcggcgtagg gcttggtggt
//
LOCUS       DASSQV010000433         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_101236, whole genome shotgun sequence.
ACCESSION   DASSQV010000433 DASSQV010000000
VERSION     DASSQV010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101236"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFL04_07560"
     CDS             complement(<1..412)
                     /locus_tag="VFL04_07560"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7839599.1"
                     /translation="MASPSGGVTLGGEGASFLAPRSLYIHVPICASKCAYCDFFSLPS
                     ASWSEAAMASLVDATLARAAVLAERFGASGFDTVYVGGGTPTALPPVLLDRLLAGIGS
                     LAGRPGEWTVEANPESLDARALETMRARGVSRISL"
     gene            complement(426..1166)
                     /gene="lepB"
                     /locus_tag="VFL04_07565"
     CDS             complement(426..1166)
                     /gene="lepB"
                     /locus_tag="VFL04_07565"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7839600.1"
                     /translation="MLWAGKRRSYAEAVENRRKTFARLLMLLMFFMAFEVLSGLFLTA
                     YSAGSTGMSPTVLPSDHLLATPLAFGPRTPFGKLPGFAKPERGDLVLVQPPYAEAPGF
                     FVDLANSFVRFVTFQRFSPFAGGASRPMAGPIIARVVGLPGDSISMEDFVFKAKAAGE
                     EHFLTEFELSSHRYDISKSALPELWQDGFPASGTMAARSLGPDEYFLAGDARSGTSDS
                     RLWGPVHADRFLAKVLLRYWPPSRFGIP"
     gene            complement(1179..2159)
                     /gene="lepB"
                     /locus_tag="VFL04_07570"
     CDS             complement(1179..2159)
                     /gene="lepB"
                     /locus_tag="VFL04_07570"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7839601.1"
                     /translation="MTAGKDLCWFLQTRTEALLTLGARRRLMRRMRQAAKSPVLDWVE
                     AVLWAACVVLIVNQYLLQAYRIPSASMADTLLVGDMIFVDKLSFGPELLPGVVKTPGL
                     AEPRRGQIVVFENPSYLQRGPLYSILQQTLYMLSLTLLDIDRDEGGSPRVHYLIKRAV
                     GMPGDRLRIERGELSLRFAGAPGWISEAEYKAGSGQDYGSHRSLEPGVYPAIEAFGRA
                     AAYGDSGLPVPAALAAMGGESAAQKDLFAFDSARVRTMYAMHPQDFRYASAARRYAAG
                     WYIPEGRVFPMGDNRDNSRDARYFGTVAERKVLGKALFIYWPLVRAGSVR"
     gene            2228..>2857
                     /locus_tag="VFL04_07575"
     CDS             2228..>2857
                     /locus_tag="VFL04_07575"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET7839602.1"
                     /translation="MLDDKRVTQEDIDSVHVRLRPFLGLEPGLWLAALYSLLIAALAF
                     LLLVLPGMRRNGSIISVDSRPSGAAVFLDGAYAGTSPCSLFAASGAHSLAVSRPGFST
                     WARRLEVGGRVFASLIAPTRVRLDARLVAAVPEANLEAAFAEYTTWSLAGRPSAIYQT
                     PLVLSDSLLALAETGNCAWPPRGGAPGFAASALAAASGEESARDALRAVF"
BASE COUNT          435 a          974 c         1031 g          417 t
ORIGIN      
        1 cgaggctgat gcggctgacc ccccgagccc gcatggtctc tagggccctg gcatcgaggc
       61 tttcggggtt ggcctcgacc gtccactccc ccggccttcc ggcgagggac ccgatgcccg
      121 cgaggagcct gtcgagcagg acggggggca gggccgtggg cgtcccgccg ccgacgtaga
      181 ccgtgtcgaa gcccgaggcg ccgaagcgct cggcgaggac ggcggccctg gcgagggtcg
      241 cgtccacgag ggaggccatg gctgcctcgg accaggacgc ggagggaagg gagaagaagt
      301 cgcagtaggc gcacttggag gcgcagatcg gcacgtggat gtagagggag cgcggggcca
      361 ggaaggaggc cccttccccg ccgagcgtca cgcccccgga cggactggcc atcccgccgc
      421 ccccatcagg ggatcccgaa gcgcgagggc ggccagtagc ggaggaggac cttcgcgagg
      481 aagcggtcag cgtggaccgg cccccagagc cgcgagtcgc tcgtgccgct ccgcgcgtcc
      541 ccggcgagga agtactcgtc agggccgagg cttctcgcgg ccatcgtgcc cgaggccggg
      601 aagccgtcct gccagagctc gggcagggcc gacttcgata tgtcgtagcg gtggctcgag
      661 agctcgaact cggtcaggaa atgctcctcg cccgcggcct tggccttgaa cacgaagtcc
      721 tccatcgaga tgctgtcccc ggggaggccg acgacgcgcg cgatgatggg accggccatc
      781 ggcctcgagg ccccgccggc gaagggtgag aagcgctgga aggtcacgaa cctgacgaag
      841 gagttggcca ggtccacgaa gaagccgggg gcctcggcgt aggggggctg gacgaggacg
      901 aggtcgccgc gctcgggctt ggcgaaaccc ggcagcttgc cgaagggcgt ccttgggccg
      961 aaggccaagg gcgtggcgag gaggtggtct gaggggagga cggtcgggct catgcccgtc
     1021 gagccggccg agtaggcggt caggaagaga cccgagagga cctcgaaggc catgaagaac
     1081 atgaggagca tgaggaggcg ggcgaaggtc ttccgcctgt tctcgacagc ctcagcgtac
     1141 gaacgtcttt tccctgccca cagcatatcc agctccgcct aacgcaccga gcccgctctg
     1201 acgaggggcc agtagatgaa gagtgccttg ccgaggacct tgcgctccgc cacggttccg
     1261 aagtagcgcg cgtcccggct gttgtccctg ttgtcgccca tggggaagac cctgccctcg
     1321 gggatgtacc agccggcggc gtagcgacgg gccgccgagg cgtagcggaa atcctgggga
     1381 tgcatcgcgt acatggtccg cacccgagcg gaatcgaagg cgaagaggtc cttctgggcc
     1441 gccgattcgc cgcccatggc cgcgagggcg gccggcacgg gaaggcccga gtccccgtag
     1501 gccgcggccc ttccgaaggc ctcgatcgcg ggatagacgc ccggctcgag gctccggtgg
     1561 ctcccgtagt cctggcccga tcccgccttg tactcggcct cggagatcca gccgggggcg
     1621 ccggcgaagc ggaggctcag ctcgccgcgc tcgatcctga gcctgtcgcc cggcatcccc
     1681 accgcgcgct ttatcaggta gtgcacccgg ggggagccgc cctcgtcgcg gtcgatgtcc
     1741 aggagggtca ggctcagcat gtagagcgtc tgctggagga tcgagtagag gggcccgcgc
     1801 tggaggtagc tcgggttctc gaacaccacg atctggccgc ggcggggctc ggccaggccc
     1861 ggcgtcttga cgacgccggg gaggagctcg gggccgaagg agagcttgtc aacgaatatc
     1921 atgtcgccga cgaggagggt gtcggccatc gaggccgagg ggatcctgta cgcctgcagg
     1981 aggtactggt tgacgatgag gacgacgcag gcggcccaga ggacggcctc gacccagtcc
     2041 agcacggggc tcttggcggc ctggcgcatc cggcgcatga gcctgcgcct ggcccccagg
     2101 gtgagcaggg cctcggtcct cgtctgcagg aaccagcaca gatccttccc ggctgtcatc
     2161 ggggtggaca ctagcacgat cgtctcgcgt tgacaagcgg gaggtcgatt cctatactcg
     2221 tttgaccatg ctcgatgaca agcgggtcac ccaggaggac atcgacagcg tccacgtgcg
     2281 cctccgcccc ttcctgggcc tcgagccggg cctgtggctc gccgcgctct actcgctcct
     2341 catcgcagcc ctcgcgttcc tcctcctcgt cctgcccggg atgcggagga acggcagcat
     2401 catctcggtg gactcacggc cctcgggcgc tgcggtcttc ctcgacgggg cctacgcggg
     2461 gacgagcccc tgctcgctgt tcgcggcctc gggcgcccac agcctcgccg tctcccgtcc
     2521 cggcttctca acctgggcca ggcggctcga ggtgggcggc agggtcttcg cgagcctcat
     2581 cgccccgacc cgggtccgcc tggacgcccg cctcgtggcg gccgtccccg aggccaacct
     2641 cgaggcggcc ttcgccgagt acaccacctg gtccctcgcg ggcaggccct cggccatcta
     2701 ccagacccca cttgtgctct cggattccct cctcgcgttg gccgaaacag gcaattgtgc
     2761 atggccgccc cgcggcgggg ctccaggctt cgcggcctcg gccctcgccg ccgcctcggg
     2821 cgaggagtcg gcccgcgacg ccctccgcgc ggtcttc
//
LOCUS       DASSQV010000434         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2341097, whole genome shotgun sequence.
ACCESSION   DASSQV010000434 DASSQV010000000
VERSION     DASSQV010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341097"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VFL04_07580"
     CDS             <1..1028
                     /locus_tag="VFL04_07580"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7839603.1"
                     /translation="MPPPGARSIGTRGRRLIEAEKAKTEARYSPMPHDAVIAAVSGLL
                     AALSFAYPDTASSFMFPAGPARGFLVGTTFILVAIAACLAPLVRAEAGALANFLRTFY
                     PLLLVFLFFQEAILLSVQLFGGRAHDAAFAAFDRALFGFQPSREFHKAFDAWPWANEL
                     MFGSYFLYYVLIGLTPLIAWALGKREEARRQIFVLSSMMALVFGFYVFFRVEGPKYWF
                     ADLRALGYSHFRGGFFTAFFQRVFTTTNLYGAAFPSTHVCFTVAMAMYARRIDRRLSL
                     PYAIAVLLVSLSTVYLYAHYVADVLGGLVAALVLVPLLSRTYPGARELCSRLGRPRGL
                     LAPEPSE"
     gene            1025..1885
                     /locus_tag="VFL04_07585"
     CDS             1025..1885
                     /locus_tag="VFL04_07585"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET7839604.1"
                     /translation="MSPVRRGRGQAFPYLGEEEAFPFPHPDTADENGIVCAGGNLSPG
                     LLLSAYRSGIFPWFSEGEPILWWSPDPRFVLFPPSLHASGTMLRVLRHGIRAGCRAGQ
                     GSSGRGAQGRGDQGDSAGRRRYELSLDRDFEAVIKSCSASPRPGQRGTWITAGMTEAY
                     IELHRLGLAHSAEARLDGRLVGGLYGVSLGSLFFGESMFSHEDDASKAAFIPLVWRLR
                     DEGFTLVDSQVYTEHLAGLGAREIPRSEYLRLLSAGLEAPTRRGNWGELFPGFPDSAE
                     YRRTVSKEQA"
     gene            1890..>2904
                     /locus_tag="VFL04_07590"
     CDS             1890..>2904
                     /locus_tag="VFL04_07590"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HET7839605.1"
                     /translation="MKILFVQLPVQDPNWENARANVSLAAGYLSAYAESLGLLARDEW
                     SLLDSGITDVGSDATIVEAITAAEADLVCFSLYMWNLERSLHVAGRVAAASPRARLVA
                     GGPEVVSGQPILDTKIFDTLIEGEGELPFCELLGDLLDGRPPKRRYAATEALDLARVP
                     NPYLAGTLGFSADRPVHLETMRGCPMHCGYCFYGKDSDELRRYPDEIAYRVIEEAGKA
                     GVRELYLMDPSFHAQSALSDRLVALAKANTAGMALNAELRLESVTEEAGRLYAAAGLV
                     SAEVGLQSINPKALEAVNRSWDRPSFERGAEILQKNRITVKTGCILGLPFDGYEQVIE
                     TFDF"
BASE COUNT          418 a         1136 c          857 g          493 t
ORIGIN      
        1 agatgccgcc ccctggcgcg cggagcatag gcacacgagg gaggcgtctc atcgaagccg
       61 agaaagcgaa aaccgaggcc cggtacagcc ccatgccgca cgacgcggtc atcgcggcag
      121 tctcgggcct cctcgccgcc ctctccttcg cctatcccga caccgcctcc tccttcatgt
      181 tccccgcggg cccggcgagg ggcttcctcg tcgggacgac cttcatcctc gtggccatcg
      241 ccgcctgtct ggcccccctc gtgagggccg aggccggggc cctggcgaac ttcctcagga
      301 ccttctaccc cctcctcctc gtcttcctct tcttccagga ggccatcctc ctctcggtcc
      361 agctcttcgg cggccgggcc cacgacgccg ccttcgccgc cttcgaccgc gcactcttcg
      421 gcttccagcc ctcgcgcgag ttccacaagg ccttcgacgc ctggccctgg gccaacgagc
      481 tcatgttcgg ctcctacttc ctctactacg tgctgatcgg cctcaccccc ctcatcgcct
      541 gggccctcgg caaacgcgag gaggcgaggc ggcagatctt cgtgctctcg tcgatgatgg
      601 cacttgtctt cggcttctac gtcttcttcc gggtcgaggg gcccaagtac tggttcgccg
      661 acctccgcgc cctcggctac tcgcatttcc gcggcggctt cttcaccgct tttttccaac
      721 gggtcttcac aacgaccaac ctctacgggg ctgccttccc ctcgacccac gtctgcttca
      781 ccgtggccat ggccatgtac gcccgccgca tcgaccggag gctcagcctc ccctacgcca
      841 tcgccgtcct cctcgtgagc ctctccaccg tctacctcta cgcccactac gtggccgacg
      901 tcctcggcgg cctggtcgcc gcccttgtcc tcgtgcccct gctttccagg acctaccccg
      961 gcgcccgcga gctctgctcg aggctgggga ggccgcgcgg actcctcgct ccggagccga
     1021 gcgaatgagc cccgtgaggc ggggacgggg ccaggccttc ccctaccttg gggaggagga
     1081 ggccttcccc ttcccccatc cggacacggc cgacgagaac ggcatcgtct gcgccggcgg
     1141 caacctctcg cccggcctcc tcctctcggc ctaccgctcg ggcatcttcc cctggttctc
     1201 cgagggcgag cccatcctct ggtggagccc cgacccccgc ttcgtcctct tccccccctc
     1261 cctccacgcg agcgggacaa tgctgcgggt gctcaggcac gggatcaggg ccggctgccg
     1321 cgcaggccag ggctccagtg gccgcggcgc ccagggccgc ggcgaccagg gcgactcggc
     1381 cggacgccgg cgctacgagc tctccctgga ccgtgacttc gaggcggtca tcaaaagctg
     1441 ttcggccagc ccgagaccgg gccagcgggg tacctggatc accgccggca tgaccgaggc
     1501 ctacatcgag ctccaccgcc tcggtctggc ccactcggcc gaggccaggc tggacgggcg
     1561 cctcgtcggc ggcctctacg gggtctcgct gggctcgctc ttcttcgggg agtcgatgtt
     1621 cagccacgag gacgacgcct ccaaggcggc cttcatcccc ctcgtgtggc ggctcaggga
     1681 cgagggcttc accctcgtcg attcgcaagt atatactgag cacctcgccg gcctcggcgc
     1741 ccgcgagatc ccccgctccg agtacctccg gcttctctcc gccgggctcg aggcgccgac
     1801 caggaggggc aactggggcg agctcttccc cggcttcccc gattcggccg agtaccgcag
     1861 gacagtctcg aaagagcaag cataaagcca tgaaaatact gttcgtccag ctgcccgtgc
     1921 aggatcccaa ctgggagaac gcccgggcca acgtgagcct cgccgccggc tacctcagcg
     1981 cctacgccga atcccttggc ctcctggccc gggacgagtg gtccctcctc gactccggca
     2041 tcaccgatgt gggctccgac gccaccatcg tggaggccat caccgctgcc gaggccgacc
     2101 tcgtctgctt ctcgctatac atgtggaacc tcgagcgaag cctccacgtc gcggggcgcg
     2161 tcgccgccgc ctcgccccgg gccaggctcg tggccggagg ccccgaggtc gtctcgggcc
     2221 agccgatctt agataccaaa atcttcgaca cactgataga gggcgagggc gagctcccct
     2281 tctgcgagct cctcggcgac cttctggacg gcagaccccc aaaacgacgc tacgccgcca
     2341 ccgaggccct cgacctcgcc cgggtgccca acccctacct cgcgggcacc ctcggcttct
     2401 cggccgacag gcccgtccac ctcgagacca tgcgcggctg cccgatgcat tgcggctact
     2461 gcttctatgg caaggattca gacgagctcc gccgctaccc ggacgagatc gcctatcgcg
     2521 tcattgaaga ggccggcaag gcgggcgtgc gcgagctgta cctcatggac ccctcgtttc
     2581 acgcgcagag cgcgctctcc gaccgcctcg tcgccctcgc caaggccaac accgccggca
     2641 tggccctcaa cgccgaactg aggctcgaat ccgtgaccga ggaagcgggc cgcctctacg
     2701 ccgcggccgg gctcgtctcg gcggaggtgg ggctgcagag catcaacccc aaggccctcg
     2761 aggcggtcaa tcgcagctgg gaccggccct ccttcgagcg cggggccgag atccttcaga
     2821 agaaccgcat cacggtgaag acgggctgca tcctcggcct gcccttcgac ggctacgagc
     2881 aggtgatcga gaccttcgat ttcc
//
LOCUS       DASSQV010000435         7289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1095137, whole genome shotgun sequence.
ACCESSION   DASSQV010000435 DASSQV010000000
VERSION     DASSQV010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095137"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2787)
                     /locus_tag="VFL04_07595"
     CDS             complement(<1..2787)
                     /locus_tag="VFL04_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839606.1"
                     /translation="MLFTTGNLITLGIVLVVLLIYRQLDRDNRSLEKVKKYAERLRDE
                     LAVYVDKRAEDLKRYGIELDVHQKAAKVALDRIQGVQEGLAARAGAIEGIEKRLGEYD
                     TALAKLMDMTARVDENLVRLHEESAFTDQVSRRLDAAQKTMAQIGQDLPALRANFAAD
                     SASTLEAFKGSLLAELGKRLDEVGSILERARSEAGASLNRAEAGRAELERELERAFER
                     ARAEAEKLEDAAFARLKEGTDIKAARLKDLVEEKFSQLGNLSKERMAEIQAQIRSFKA
                     EWKAESDELAAGSREELAAIAQAARAETTRAVEAVKTETFAMVAAARTEAFEMISSAK
                     DAQENMLASAREETAGTLQSLASETEALIANARTDITDATERLGARVDEIDARVLAAE
                     RLYDERFEKVELRAEELAAGLVEKTKALLSEQRRELERIAAESGEAATAIERMAQDRT
                     EALDRRMAEHEAALVARIAGLDHQIAEHKAALAAKLAGLDRQIAEHETALGGKLVGLD
                     RQIADHETAIAARIAGLDQHIDDHDAAVAARIAGIEGQMDEGERRLRARTEAAAAAQA
                     EAIESLGREGQRLIEAAGESSAERVEQLRQRSEQLVSQLLERTKAGLAEQAALLKAAG
                     AEQAAIIEAYRADSAASAKTTQAELARTEASLSETAARTSREMAALETRLATEGDALA
                     TRAFEEFGRRLAEYGSEAEARFERLEAAGAEIASLDSALRTSMDQVARRIESDFAAFG
                     TALEDRRSRFEEGFLAESAKLRGGMGALEDELAALKTRAYDNVSEKLKVFEDEFFVDL
                     KTRGEGIEARLEAWRSDLDKTLSDLAARAASDRSAAERASAEEMRGHLAETQARLQDQ
                     LATLRDRVGAVQDGIQAQSGMAVEALAALKDSVLKEAAEARATAQAYVEGELSRFSLE
                     ANARLKAAERDVEGR"
     gene            2926..4035
                     /locus_tag="VFL04_07600"
     CDS             2926..4035
                     /locus_tag="VFL04_07600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7839607.1"
                     /translation="MSARARCSLPLFIAALLVLGPQTLTALPGAVAAEPGAAAAGPGP
                     AGAPAPSAAPAPAPAGNAAAQGAVLVAPDSLRQGDPLLAWVISAEAGAYEARLESEAG
                     GTAATAPSFDASSLAPGPKASKLRLSGLLIPIPMDFKPGPYRLVVRGPRSVAGSEPPE
                     LVHRLSIAARAFPLESIPLDAKNAELLTAPDPRKEAEAKAFNAIFATVDPEAGFLDGS
                     AFSLPVDSKRRTAGFGDKRRYLYPSGGSDSSVHQGIDFAAPQGSPVLASARGKVVFAA
                     ERIVTGNTVVIEHLPGLYSIYMHFSKIEAAEGQMVERGQRIGLSGSTGLSTGPHLHWE
                     LRARGLAVDPEFWLARPPLDNETLIAKMAGLIEGR"
     gene            4041..4253
                     /gene="rpsU"
                     /locus_tag="VFL04_07605"
     CDS             4041..4253
                     /gene="rpsU"
                     /locus_tag="VFL04_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET7839608.1"
                     /translation="MKQIIVDEGEPLEKAIKRFKRMVEKEGIIREWKKREFFEKPSTV
                     KNRKMKALARKLMKKARKPRDSKGGY"
     gene            4389..4883
                     /locus_tag="VFL04_07610"
     CDS             4389..4883
                     /locus_tag="VFL04_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET7839609.1"
                     /translation="MIMVKKTRPWLGRLALACLAASLFALASCAAKGAEAEPTRPNPK
                     LKTVELKIGDASVQAELARTSLERETGLMFRTSLAEGTGMLFVFEADQQLAFWMKNTK
                     LPLSLAYISSDGTIREIFDLVPGSLDPVQSERSVRYALEVPRGWFAKAGIKSGDRVAI
                     PPLD"
     gene            4938..5426
                     /gene="def"
                     /locus_tag="VFL04_07615"
     CDS             4938..5426
                     /gene="def"
                     /locus_tag="VFL04_07615"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7839610.1"
                     /translation="MLEIVTYGNEILNRKGQTVTDFGPELSAFVSDMHEAMRIGKGIG
                     LAAPQVNSPLRVFVTHVEDDKPRVFINPEIVLTSQEEVEFEEGCLSLPGLYLSLKRPE
                     MVRIQAYNEKGKPFSIEAGGILARVMLHEYDHLEGVLFIDRLPPAKREKALAHYRRLL
                     RM"
     gene            5431..6429
                     /gene="fmt"
                     /locus_tag="VFL04_07620"
     CDS             5431..6429
                     /gene="fmt"
                     /locus_tag="VFL04_07620"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET7839611.1"
                     /translation="MLRVLFAGSPDIAIPSLVRLSFEQEIVGILTNPDTQRGRGLAVR
                     ETPVAQAALAVFHGQIPILAFEKLGAEARDAVAALKPDVLVSYAYGKIFGPRFLALFP
                     KGGINVHPSLLPKYRGPAPIPYAILNREAETGISVQRLALRMDEGELLGVERIRLLGL
                     ENAEDLAETAAIVGADLLSRVLQEMAEGREHSQPQEGEPSYCKLLRKEDGNLDWTRPV
                     LELDAKVRAFYPWPGAFTFLGGQRLNVLEAYPYPHVTFAAGPTSLDFDEVVPGRVLGL
                     DKARGIMVQTKDGLMALRRLQIQHKRALPYREFANGIRDLVGAVLGGPSPEESDRP"
     gene            6426..>7289
                     /locus_tag="VFL04_07625"
     CDS             6426..>7289
                     /locus_tag="VFL04_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET7839612.1"
                     /translation="MKLKFKFPKMPRFSRLKDLRFPDLPLDLRKLENLDRDHYRMAVY
                     ALSAIIVLMVVAGFTAFVLSLRGEEQTMVPDLTGMELSQALIKLQEKELYPRIALRFT
                     DNPNDRGKIVEQRPEAGAIVKAGRRIQLSVSRGAVVDRVENFVGQDLNEVKIHLQTLF
                     ASTRALLVIKEPPIYLFDKAPAGTILEQKPAPETELSSPVPLELVVSRGPEKAQIRVP
                     DLVGLSISDAILQVEKTDLPVKFTMRAAGRSERPGSVVAQLPAPGTTIAAVARVTVTL
                     AAPAAEKGTVAG"
BASE COUNT         1042 a         2628 c         2418 g         1201 t
ORIGIN      
        1 cctgccctcg acatcgcgct cggcggcctt gagcctggcg ttggcctcga gcgagaagcg
       61 ggagagctcg ccctcgacgt aggcctgggc cgtggccctg gcctcggcgg cctccttgag
      121 gaccgagtcc ttgagggcgg cgagggcctc gacggccatg ccgctctggg cctggatccc
      181 gtcctggacg gcgcccacgc ggtcgcggag ggtggccagc tggtcctgga gcctggcctg
      241 ggtctcggcg aggtggccgc gcatctcctc ggccgaggcc cgctcggcgg cgctgcggtc
      301 cgaggccgcc ctggccgcga ggtcggagag ggtcttgtcg aggtcggagc gccaggcctc
      361 gagccgggcc tcgatgccct cgcccctggt cttgaggtcg acgaagaact cgtcctcgaa
      421 gaccttgagc ttctcggaga cgttgtcgta ggccctcgtc ttgagggcgg cgagctcgtc
      481 ctcgagggcg cccatgcccc cgcggagctt ggcgctctcg gcgaggaagc cctcctcgaa
      541 gcgggagcgc ctgtcctcaa gggctgtgcc gaaggctgcg aagtcgctct cgatcctgcg
      601 cgcgacctgg tccatcgagg tcctgagggc cgagtcgagg ctggcgatct cggccccggc
      661 ggcctcgaga cgctcgaagc gggcctcggc ctcggagccg tactcggcga gccgtcggcc
      721 gaactcctcg aaggccctgg tggccagggc gtccccctcg gtggcgaggc gggtctcgag
      781 ggcggccatc tccctcgagg tgcgggcagc ggtctcggag agcgaggcct cggtgcgggc
      841 gagctcggcc tgggtcgtct tcgcggacgc ggcgctgtcc gccctgtagg cctcgatgat
      901 ggcggcctgc tcggcgcccg cggccttgag gagggccgcc tgctcggcga ggcctgcctt
      961 tgtgcgctcg aggagctggg agacgagctg ctcgctcctc tgcctgagct gctcgacgcg
     1021 ctcggccgag gattcgcccg cggcctcgat gaggcgctgg ccctcgcggc caaggctctc
     1081 gatggcctcg gcctgggcgg cggcggcggc ctcggtcctc gccctgagcc gtctctcccc
     1141 ctcgtccatc tggccttcga tgcccgcgat cctcgcggcg acggcggcat cgtggtcgtc
     1201 aatatgctgg tcaaggccgg cgatcctggc cgcgatggcg gtctcgtgat cggcgatctg
     1261 ccggtcgagg ccgacgagct tgccgccgag ggcggtctcg tgctcggcga tctgccggtc
     1321 gaggccggcg agcttcgcgg cgagggctgc cttgtgctcg gcgatctggt ggtcgaggcc
     1381 ggcgatcctg gcgacgaggg ctgcctcgtg ctcggccatc ctccggtcga gggcctcggt
     1441 cctgtcctgg gccatgcgct cgatggccgt ggccgcctcg ccgctctcgg cggcgatccg
     1501 ctcgagctcg cggcgctgct ccgaaaggag ggccttggtc ttctcgacga gaccggcggc
     1561 cagctcctcg gcccgcagct cgaccttctc gaagcgctca tcgtaaaggc gctcggcggc
     1621 gaggacccgc gcgtcgatct cgtccaccct ggcgccgagg cgctcggtgg cgtcggtgat
     1681 gtcggtcctc gcgttcgcga tgagggcctc ggtctcggag gcgaggctct ggagggtccc
     1741 cgcggtctcc tctcgcgcgc tcgcgagcat gttctcctgc gcgtccttgg cgctcgagat
     1801 catctcgaag gcctcggtcc tcgccgccgc gaccatggcg aaggtctcgg tcttgaccgc
     1861 ctcgacggcc ctcgtggtct cggccctggc cgcctgggcg atcgccgcca gctcctcgcg
     1921 ggagcccgcg gcgagctcgt cggactcggc cttccactcg gccttgaagc tcctgatctg
     1981 ggcctggatc tcggccatcc tctccttcga gaggttcccg agctgggaga acttctcctc
     2041 gacgaggtcc ttgagccggg cggcctttat gtcggtgccc tccttcagcc tcgcgaaggc
     2101 ggcgtcctcg agcttctcgg cctcggccct ggcccgctcg aaggcgcgct cgagttcgcg
     2161 ctcgagctcg gccctgccgg cctcggcgcg gttcaggctg gccccggctt cgctgcgggc
     2221 ccgctcgagg atggagccca cctcgtcgag ccgcttgccc agctcggcga gcaggctgcc
     2281 cttgaaggcc tcgagggtcg aggcgctgtc ggccgcgaag ttggccctga gggctggcag
     2341 gtcttggcca atctgggcca tggtcttctg cgcggcgtcg agcctgcggc tgacctggtc
     2401 ggtgaaggcc gactcctcgt ggagcctgac gaggttctcg tcgacccggg cggtcatgtc
     2461 catgagcttg gcgagggccg tgtcgtactc gccgagccgc ttctctatgc cctctatcgc
     2521 ccccgcccgc gccgccaggc cctcctgcac cccctggatc ctgtcgaggg ccaccttggc
     2581 ggccttctgg tgcacgtcga gctcgatccc gtagcgtttg aggtcctcgg cccgcttgtc
     2641 gacgtagacg gccaactcgt cgcggagccg ttccgcgtac ttcttcacct tctcgaggga
     2701 gcggttgtcc cggtcgagct ggcggtagat caggaggacc accaggacga tgccgagggt
     2761 tatcaggttt cccgtcgtga agagcatgcg gaactccatg gtttcggatg gatatacgtc
     2821 gcggattata gacgatattc gcggcgaacg ctacgccgcg gcccggtcta aaggcgcagc
     2881 ccatcaggct tctcgcgcag gggcgtttcc ggtatcttcc ctgatatgtc agcgcgagcc
     2941 cggtgcagtc ttccgctctt catcgccgcc ctcctcgtcc tgggccccca gaccctcacc
     3001 gccctgcccg gagcggtcgc cgcagagcct ggagcggccg ccgccggccc cggcccggcg
     3061 ggggcccccg cgccgtcagc agcgcccgcg cccgcccccg cgggcaacgc ggcggctcaa
     3121 ggcgccgtcc tcgtcgcgcc cgacagcctc cggcagggcg atcccctcct cgcctgggtc
     3181 atctcggccg aggccggtgc ctacgaggcg cggctggaaa gcgaggccgg aggcacggcg
     3241 gcgacggccc cctccttcga cgcctcctcg ctcgccccgg gcccgaaggc gtccaagctc
     3301 aggctctcgg gcctcctcat ccccatcccg atggacttta agcccggccc ctaccgcctc
     3361 gtcgtccgcg gcccccgttc ggtggccggc tcggagccgc ccgagctcgt gcatcgcctc
     3421 agcatcgccg cgcgcgcctt ccccctcgag agcatccccc tggacgccaa gaacgccgag
     3481 ctcctcaccg cccccgaccc gcgcaaggag gccgaggcga aggccttcaa cgccatcttc
     3541 gccacggtcg accccgaggc gggcttcctt gacggctcgg ccttctccct gccggtggac
     3601 tcgaagcgca ggacggcggg cttcggcgac aagcggcgat atctctatcc gagcggaggc
     3661 tcggactcga gcgtccatca gggcatcgat ttcgccgcgc cccagggcag ccccgtcctc
     3721 gcctcggccc ggggcaaggt ggtcttcgcg gccgagcgca tcgtgaccgg caacaccgtc
     3781 gtcatcgagc atctgcccgg gctctactcg atctacatgc atttctccaa gatcgaggcg
     3841 gccgagggcc agatggtcga gcggggccag aggatcgggc tctcgggctc gacggggctc
     3901 tcgacggggc cccacctcca ttgggagctc agggcgaggg gcctcgccgt cgatcccgag
     3961 ttctggctgg cccgccctcc ccttgacaac gagaccctaa tcgctaaaat ggctgggttg
     4021 atcgaaggga ggtgatgttt atcaagcaga tcatcgtgga cgaaggcgag cccctcgaga
     4081 aggccatcaa gcgcttcaag cgcatggtgg agaaggaggg gatcatccgc gagtggaaga
     4141 agcgcgagtt tttcgagaag ccttccacgg tcaagaaccg caagatgaag gcgctggccc
     4201 gcaagctcat gaagaaggct cgcaagcccc gcgactccaa gggcggctac taagaaccct
     4261 tcgatccgga tccattaggt ccaggaccgc gagagcctcg gtcaagcatg acggcagtcc
     4321 cgcttgaccg acgctttcgc gggttttttt tgcgcctcac ggggcgcgcg tcgttgaaag
     4381 gaagagcgat gatcatggtc aagaagaccc ggccctggct cgggcggctt gccctggcct
     4441 gtctggccgc ctccctcttc gccctcgcgt cctgcgcggc gaagggggcc gaggccgaac
     4501 ccacgaggcc gaaccccaag ctcaagaccg tggagctcaa gatcggcgat gccagcgtgc
     4561 aggccgagct cgcgaggacc agcctcgagc gcgagaccgg gctcatgttc aggaccagcc
     4621 tggccgaggg cacgggcatg ctcttcgtct tcgaggccga ccagcagctc gccttctgga
     4681 tgaagaacac gaagctgccc ctgtccctcg cctacatctc ctcggacggg acgatccgcg
     4741 agatcttcga cctcgtgccc ggctccctcg atccggtgca gtcggagcgc tcggtccgct
     4801 atgccctcga ggtgccgcgg ggctggttcg cgaaggccgg gatcaagagc ggcgacaggg
     4861 tggccattcc gccgctggac tgaggttgac cggtgcaggg gccagtggct acattcggtc
     4921 caagggggag cttccgcatg ctcgagatag tcacgtacgg gaacgaaata ctcaacagga
     4981 agggccagac ggtgaccgat ttcggccccg agctctcggc cttcgtctcg gacatgcatg
     5041 aggcgatgcg gatcggcaag ggcatcggcc tcgccgcccc gcaggtgaac agccccctcc
     5101 gcgtcttcgt cacccatgtc gaggacgaca agcccagggt cttcatcaac cccgagatcg
     5161 tcctgacctc ccaggaggag gtcgagttcg aggagggctg tctctccctg ccggggctct
     5221 acctctcgct caagcggccc gagatggtgc ggatccaggc ctacaacgag aagggcaagc
     5281 ccttctcgat cgaggccggg ggcatcctgg cccgggtcat gctgcacgag tacgaccatc
     5341 tcgagggcgt gctcttcatc gaccgcctgc ctcccgccaa gcgggagaag gccctggcgc
     5401 actaccgccg cctcctccgc atgtagggcc gtgctgcgcg tcctgttcgc gggcagtccc
     5461 gacatagcca taccctccct cgtccgcctg tccttcgagc aggagatcgt cggcatcctc
     5521 accaaccccg acacccagag gggcaggggg ctcgccgtgc gcgagacccc ggtggcccag
     5581 gccgccctgg ccgtcttcca cggccagatc ccgatcctcg ccttcgagaa gctcggcgcc
     5641 gaggcgaggg atgcggtggc cgccctgaag cccgacgtcc tcgtgtccta cgcctacggc
     5701 aagatcttcg gcccccgctt cctcgccctc ttcccgaagg gcgggatcaa cgtccatccg
     5761 agcctcctgc ccaagtaccg gggcccggcc cccatcccct acgccatcct caaccgcgag
     5821 gccgagacgg gcatcagcgt ccagcgcctc gccctgcgga tggacgaggg cgagctcctc
     5881 ggcgtcgaga ggatccgcct cctcggcctc gagaacgccg aggacctggc cgaaacggcg
     5941 gccatcgtcg gggccgacct cctgtccagg gtcctccagg agatggccga gggcagggag
     6001 cacagccagc cccaggaggg cgagccgagc tactgcaagc tcctccgcaa ggaggacggc
     6061 aacctcgact ggaccaggcc cgtcctcgag ctcgacgcga aggtcagggc cttctacccc
     6121 tggcccgggg ccttcacctt cctgggcggc cagcgcctca acgtccttga ggcctacccc
     6181 tatccccatg tcaccttcgc ggccggcccg accagcctcg acttcgacga ggtggtgccc
     6241 gggagggtcc tcggtctgga caaggctcgg ggcataatgg tacaaacgaa agacggcctc
     6301 atggccctca ggcgtctcca gatccagcat aaaagggccc ttccctacag ggagttcgcc
     6361 aatgggatcc gcgatctcgt gggcgccgtc ctgggaggcc cgtcgccgga ggaatcggac
     6421 cgcccatgaa gctcaaattc aagttcccaa aaatgcccag attctcgagg ctcaaggacc
     6481 tccgcttccc tgacctcccc ctcgacctgc gcaagctcga gaacctcgac cgcgaccatt
     6541 accgcatggc cgtctatgcc ctgtcggcca tcatcgtcct catggtcgtg gcgggcttca
     6601 ccgccttcgt cctctccctc aggggcgagg agcagaccat ggtccccgac ctcacgggca
     6661 tggagctctc ccaggccctc atcaagctcc aggagaagga gctctacccg cggatcgccc
     6721 tccgtttcac cgacaacccg aacgaccgcg gcaagatcgt cgagcagagg cccgaggccg
     6781 gggccatcgt caaggcgggc aggcggatcc agctcagcgt gagccgcggc gccgtcgtgg
     6841 acagggtgga gaacttcgtg ggccaggacc tcaacgaggt gaagatccac ctccagaccc
     6901 tcttcgcctc gacccgggcc ctcctcgtga tcaaggagcc gccgatctac ctcttcgaca
     6961 aggcgcccgc gggcaccatc ctcgagcaga agccggcccc cgagaccgag ctctcgagcc
     7021 ccgtgcccct cgagctcgtg gtgagccggg ggcccgagaa ggcccagatc cgcgtgcccg
     7081 acctcgtcgg cctttccatc tccgacgcca tcctccaggt cgagaagacc gacctgccgg
     7141 tcaagttcac aatgagggcc gccggcagga gcgagaggcc cggcagcgtg gtcgcccagc
     7201 ttcccgcccc cggcacgacg atcgcggccg tggccagggt cacggtgacc ctcgccgccc
     7261 ccgccgccga gaaggggacg gtcgctggc
//
LOCUS       DASSQV010000436         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1718004, whole genome shotgun sequence.
ACCESSION   DASSQV010000436 DASSQV010000000
VERSION     DASSQV010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718004"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFL04_07630"
     CDS             complement(<1..576)
                     /locus_tag="VFL04_07630"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET7839613.1"
                     /translation="MEQETMRGYAAVVLDAGLGIGPGSMLRIMGEFPQRELMYVIAQE
                     AYDRGAKFVRIEYDDMRLARIRADRSRDEYIDQLSALTEKDSQVYVEEGWSFLRLDGY
                     ESASAMEGVDHGRLTRIQRARGPAVRVLREAQMASRLPWCVVPAPTEAWAKTVFGEAG
                     TAERLWRSLVPILRLDAPDPAAALSAHMRALE"
     gene            complement(706..1173)
                     /locus_tag="VFL04_07635"
     CDS             complement(706..1173)
                     /locus_tag="VFL04_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET7839614.1"
                     /translation="MEFAKLPPGSIVLVDTAAFVYLLEGSGARRRVVEASFASARERG
                     LDLIASTIVWTELLDGAGPRAELAAGYRSLLADSARIRLIGVDVAVAEEAAGLLAAAR
                     AAGRPLGLADSLHIATAIVMGAKAVLGNDEAWRQVPPCPPLLLIDELAFDLEA"
     gene            complement(1176..2066)
                     /locus_tag="VFL04_07640"
     CDS             complement(1176..2066)
                     /locus_tag="VFL04_07640"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET7839615.1"
                     /translation="MDDALFINIVDIISEGVYYVDLNQRITFWNRGAERITGFSRVEM
                     LGENCGGNTICDHGGENASACPLAEVMRDGRTRENDIFFRHKDGHRIPVSLRSTPLRD
                     ARGAIVGAIEIFSDRSARGSLIAELERLKKEALTDPLTGLGNRRFVEMSLEARFEALA
                     KDSVPFGVIMFDIDRFKSVNDLHGHEAGDRVLRAVADSISTSLRRLDAAARWGGEEFL
                     VLSPNVSSEILGQIAERVRQSVLAERIELPGGDCLGVTVSGGGAIARPKEGPIELVAR
                     ADLRLYQCKESGRNRSLVGD"
     gene            2164..>2780
                     /locus_tag="VFL04_07645"
     CDS             2164..>2780
                     /locus_tag="VFL04_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839616.1"
                     /translation="MGEARGFIVAAWEERGQAGPRGGPRPRRHLFSGRLEDGRSFAAI
                     RKAPPPAILVAASQAERAMAVLGTSLGAGLSLDPEPWSDMAGTALARLSLPEGSLPQA
                     ERELGRAGIVAAGRDRMRADEALAALGVRGPVRLRGEVVAGKRVDLVFLEPELEAVEH
                     EAELGWLALDIETDREDRVVAVSLAGVGGEGEVLFWGPELGSPRIR"
BASE COUNT          388 a          969 c         1019 g          404 t
ORIGIN      
        1 ctcgagggcc cgcatgtggg ccgagagggc cgccgcgggg tcgggggcgt cgaggcggag
       61 gatggggacc aggctcctcc agagcctctc cgccgtcccg gcctcaccga agaccgtctt
      121 ggcccaggcc tcggtggggg cgggcacgac gcaccagggc agacggctgg ccatctgggc
      181 ctcgcggagg acgcggacag ccgggccgcg ggcgcgctgt atgcgcgtta ggcggccgtg
      241 gtcgacgccc tccatggccg acgcgctctc gtagccgtcg aggcggagga agctccagcc
      301 ctcctcgacg tagacctggc tgtccttctc ggtgagggcg ctcagctggt cgatgtactc
      361 gtcgcgggag cggtcggccc tgatcctggc gagccgcatg tcgtcgtact cgatcctcac
      421 gaacttcgcg ccgcggtcgt aggcctcctg ggcgatcacg tacatgagtt cgcgctgggg
      481 gaactccccc atgatcctca gcatcgagcc gggcccgatc ccgagcccgg cgtccagcac
      541 cacagcggcg tagccgcgca tcgtttcctg ttccatgtgc cgatactagc acggccgagg
      601 accgggccgc gaggcccgac ccaggcggca aggaggggct cgcgggcgag gcccgaccct
      661 ggcggcgggg agcggaccgc gcggcgaggc ccggcccttg ccgcctcagg cctcgaggtc
      721 gaaggcgagc tcgtcgatga ggaggagggg agggcagggc ggcacctgcc gccaggcctc
      781 gtcgttgccg agcacggcct tcgcccccat gacgatggcc gtggcgatgt ggagggagtc
      841 ggcgaggccc aggggccgcc ccgcggcccg cgccgccgcg aggaggccgg ccgcctcctc
      901 ggcgaccgcc acgtcgacac cgatcagcct gatcctggcc gagtcggcga ggaggctgcg
      961 gtagccggcc gcgagctcgg ccctcggccc ggccccgtcg aggagctcgg tccagacgat
     1021 ggtagaggcg atgaggtcga ggcccctttc cctggccgag gcgaaggagg cctcgacgac
     1081 gcgccggcgg gcgcccgatc cctcgagcag gtagacgaag gccgccgtgt cgacgaggac
     1141 gatcgagcct ggagggagct tcgcgaactc catgctcagt cgccgaccag gctccggttc
     1201 ctgccgctct ccttgcactg gtagaggcgg aggtctgccc tggccacgag ctcgatgggg
     1261 ccctccttcg ggcgggctat cgccccgccg ccggagacgg tgacgccgag acagtccccg
     1321 cccggcagct cgatccgctc ggcgaggacg ctctggcgca cgcgctcggc gatctggccg
     1381 aggatctcgg acgagacgtt cggggagagc acgaggaact cctcgccgcc ccagcgcgcc
     1441 gccgcgtcca gcctgcggag ggaggtcgag atggagtcgg cgacagccct gagcacccgg
     1501 tcgccagcct cgtggccgtg caggtcgttc acgctcttga acctgtcgat gtcgaacatg
     1561 atgaccccga aggggaccga gtccttcgcg agggcctcga acctcgcctc gaggctcatc
     1621 tcgacgaagc gccggttgcc gaggccggtc aggggatcgg tgagcgcctc cttcttgagg
     1681 cgctcgagct cggcgatgag gctgccccgg gcgctccggt ccgagaagat ctcgatggcc
     1741 ccgacgatcg cccccctcgc gtcgcggagc ggggtcgacc tgagcgaaac ggggatgcgg
     1801 tgcccatcct tgtggcggaa gaagatgtcg ttctcgcggg tcctgccgtc gcgcatgacc
     1861 tcggcgaggg ggcaggccga ggcgttctcc ccgccgtggt cgcagatcgt gttgccgccg
     1921 cagttctcgc cgagcatctc gaccctggag aagccggtga tccgctccgc tcccctgttc
     1981 cagaaggtaa tgcgctggtt caggtcgacg tagtagactc cctcggagat gatgtccacg
     2041 atattgatga aaagcgcgtc atccatcctg gcccacctcc tttccgtttg agtatagcca
     2101 ctccctccgg gcctcggaaa ggtcggctcg gccgctttgc ccgcttgtgg tatattcgac
     2161 cggatgggcg aagccagggg tttcatcgtc gcggcctggg aggagcgcgg ccaggccggg
     2221 ccgcgtggcg gaccgaggcc gaggcgccac ctcttctcgg gcaggctcga ggacgggcgc
     2281 agcttcgccg cgattcgcaa ggcccctccc cccgccatcc tcgtggcggc cagccaggcc
     2341 gagcgggcca tggccgtcct cggcaccagc ctcggagcag gcctgagtct cgaccccgag
     2401 ccctggtcgg acatggccgg aaccgctctc gcgcggctca gcctgcccga gggcagcctt
     2461 ccccaggccg agcgcgaact cgggagggcc ggcatcgtgg ccgccggacg ggacaggatg
     2521 agggccgacg aggccctcgc cgccctcggg gtccgcggac ccgtccgcct caggggggag
     2581 gtggtcgcgg gcaaacgcgt cgacctggtc ttcctcgagc ccgagctcga ggcggtcgag
     2641 cacgaggccg agctcggctg gctcgccctg gacatcgaga ccgaccgcga ggacagggtg
     2701 gtcgccgtct ccctcgccgg agtcggcggg gagggggagg tccttttctg ggggccagag
     2761 ctcggctcgc ccaggatccg
//
LOCUS       DASSQV010000437         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2466113, whole genome shotgun sequence.
ACCESSION   DASSQV010000437 DASSQV010000000
VERSION     DASSQV010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2466113"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFL04_07650"
     CDS             <1..229
                     /locus_tag="VFL04_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839617.1"
                     /translation="IGSLSSDPEVLKATPFLPAVMAELSNGKIDPPLKNFQALKDALV
                     VNLSALASTDITPLEAMKRAQDKLKDVDFSK"
     gene            353..1258
                     /locus_tag="VFL04_07655"
     CDS             353..1258
                     /locus_tag="VFL04_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7839618.1"
                     /translation="MRKSFCWSLERGDLLPVILMAPAVLLVFCVLVLPLCYGIFLSFF
                     DFGFGGFVPSRDFVGLKHYAAFLRDTTALRAVANTLAFSAGAIAGDFVIGTAAAVLIF
                     QLSRRVAAVVRPLVTVPLLISPIVVGLIWRYMYDPRGLVYWLLGRVGVTIDMFPGVTA
                     ASTALLSTVIAHWWQVVPFVVIVITAGLVSIPVELYEAAYADGAGAFTSFWRITFPLL
                     REVYVVILLISGVDTIKVFDIVFALTGGGPNDSTVTTSIYAYSQGFSNSNLSYAMTIS
                     VVAMLVTFLVFGLPFIRHNRARAAR"
     gene            1277..2107
                     /locus_tag="VFL04_07660"
     CDS             1277..2107
                     /locus_tag="VFL04_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7839619.1"
                     /translation="MVSKNTWWFRILRAALLAGLLFWTLAPVYIVVSNAFRRTLDIKK
                     MPPELLFKPIALHFQRLFELDDFLKYFANSIVISASVTLVTLALGTLAAYGMKLFRSR
                     IGERLSNFLLIGKLVPGITILIPLYIMLNRAHITGTYLGPILAHSAMGLPFITWLMTS
                     FVWDIPKETIESACIEGCSRMRTFWHVVFPMLTPALASAAILIMQSSWNELLFSLQLT
                     NINTYPLTVGIARYVGAISVDWGKCSAAATISMLPIIAAGFFMQRYLVAGMTAGAVKG
                     "
BASE COUNT          334 a          850 c          598 g          419 t
ORIGIN      
        1 gatcggctcc ctctcctccg atcccgaggt cctcaaggcc acgcccttcc ttcctgccgt
       61 catggccgag ctctccaacg gcaagatcga tcccccgctc aagaacttcc aggccctcaa
      121 ggacgcactt gtcgtcaacc tctcggccct cgcctccacc gacatcacgc ccctcgaggc
      181 catgaagagg gcccaggaca agctcaagga cgtcgacttc agcaaataga cacaggggcg
      241 cgccgccggt atcctgccgg cggcggcgcc catcgcccgg ggcccgaggc atcgggcccg
      301 ggcggccccg gacgccgggg accactccac cgagcctccg cgaggtatgc agatgcgcaa
      361 gtccttttgc tggtcactcg aacgggggga cctcctgccc gtcatcctga tggccccggc
      421 cgtcctcctc gtgttctgcg tcctcgtcct gcccctgtgc tacgggatct tcctcagctt
      481 cttcgacttc ggcttcggcg gcttcgtgcc ctcgcgggac ttcgtcggcc tcaagcacta
      541 cgccgccttc ctgcgcgaca ccaccgccct ccgcgccgtc gccaacaccc tcgccttctc
      601 ggcgggcgcc atcgccggcg acttcgtcat cgggacggcc gccgccgtcc tcatcttcca
      661 gctctcgcgc cgcgtggccg ccgtggtcag gcccctcgtg accgtacccc tcctcatctc
      721 gcccatcgtc gtcggcctca tctggcgcta catgtacgac ccccgcggcc tcgtctactg
      781 gctcctcggc agggtgggcg tcacgatcga catgttcccc ggcgtgacgg ccgcgtccac
      841 ggccctcctg tccaccgtca tcgcccactg gtggcaggtc gtgcccttcg tcgtcatcgt
      901 catcaccgcc ggcctcgtgt cgataccggt ggagctctac gaggccgcct acgccgacgg
      961 ggccggagcc ttcacctcgt tctggaggat caccttcccc ctgctcaggg aggtctacgt
     1021 ggtcatcctc ctcataagcg gcgttgacac catcaaggtc ttcgacatcg tcttcgccct
     1081 caccggaggg gggccgaacg actccacggt gacgacgagc atctacgcct acagccaggg
     1141 cttctccaac tcgaacctga gctacgccat gaccatctcc gtcgtcgcga tgctcgtgac
     1201 cttcctggtc ttcggcctgc ccttcatcag gcacaaccgg gccagggccg cacgctagac
     1261 ccgccggagg aacacgatgg tctccaagaa cacctggtgg ttcaggatcc tgagggcggc
     1321 actgctcgcc ggcctcctgt tctggaccct ggcccccgtc tacatcgtcg tctcgaacgc
     1381 cttcaggcgc accctcgaca tcaagaagat gccgcccgag ctcctgttca agccgatcgc
     1441 cctgcacttc cagcgtctct tcgagctcga cgacttcctt aagtacttcg cgaactcgat
     1501 cgtcatctcg gcctcggtga ccctggtgac cctggctctc ggcaccctcg cggcctacgg
     1561 gatgaagctc ttccgctcgc ggatcggcga gcggctctcg aacttcctac tcatcggcaa
     1621 gctcgtcccg gggatcacca tcctcatccc cctgtacatc atgctcaacc gggcccacat
     1681 caccggtacc tacctcgggc ccatcctcgc ccattcggcg atgggcctgc ccttcatcac
     1741 ctggctcatg acgagcttcg tctgggacat tcccaaggag accatcgagt cggcctgcat
     1801 cgagggctgc tcgcggatga ggaccttctg gcacgtcgtc ttccccatgc tcacaccggc
     1861 cctcgcctcg gccgccatcc tcatcatgca gtcctcgtgg aacgagctcc tcttctcgct
     1921 ccagctgacc aacatcaaca cctaccccct caccgtgggc atcgcgcgct acgtcggggc
     1981 gatctcggtg gactggggca agtgcagcgc cgcagccacc atatcgatgc tgcccatcat
     2041 cgcggcgggc ttcttcatgc agcgctacct cgtcgccggc atgacggccg gcgcggtcaa
     2101 gggctaggag gcgaagcttg tacttcggga gccaatacta cagaccgccc ttcccggcga
     2161 gggaggactg ggagcgcgac ctcggtcaca tgcgcgagct a
//
LOCUS       DASSQV010000438         5450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2342107, whole genome shotgun sequence.
ACCESSION   DASSQV010000438 DASSQV010000000
VERSION     DASSQV010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342107"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFL04_07665"
     CDS             complement(<1..612)
                     /locus_tag="VFL04_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HET7839620.1"
                     /translation="MSDTTTSLQEEAPVDAQEIMKKFDKEADYRDLTGVIARIVAAIA
                     ISFSLFQIYTALFGVFDAMIQRSVHLGFAFCLIYLLYPTSKKWSRKKLHPVDAVLALL
                     GAASPLYVTVLWTKVLSQRAGTATPLDIIVGVIGVLLVLEAARRVVGLPIVIVAMAFI
                     AYAFAGPYLPGRFAHRGVTLESLTEHFYYTTEGVFGTPIGVSST"
     gene            complement(619..1122)
                     /locus_tag="VFL04_07670"
     CDS             complement(619..1122)
                     /locus_tag="VFL04_07670"
                     /inference="COORDINATES: protein motif:HMM:NF020479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HET7839621.1"
                     /translation="MAAKRWAALALAVPGLAAFLVLAHPRLVPLPELLVSEPGGRLLA
                     AIELPDGRFDHVFVHSVHRSEVVERFAIEASGRGRATLRLYELRYENQGVGMPSDAEG
                     GYRLEDGRFVLSMDRRFEKIPIFVSVLPGHGLLANGAYLPFRNWARPETLIVLSAKTR
                     THILLGR"
     gene            complement(1131..2078)
                     /locus_tag="VFL04_07675"
     CDS             complement(1131..2078)
                     /locus_tag="VFL04_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET7839622.1"
                     /translation="MNKKLIAIALCLLVAASLGFGQQKFITIATGGTAGTYFPLGGAL
                     AEIWNKNIPGANASATVTGASIANVAMLGKGEVDVIFVQNDGAFYALNGIELFKDKPL
                     KDIRGLACLYNETVQLVALADSGIKSVADLRGKRVSVGAAGSGVEIHARQVLEAAGLS
                     YKDIKVQYLSFAESASNLKDGNIDAAFNTAGAPTAAIQDLGASKKIVLVPIDGAIAAT
                     LMKKWSFYTPEKIPANTYQGVTSDVMTVSVKSMLAVKAGMDPQLVYDLLKTMYANGER
                     LAASHKKGADIKLLTSLDGMSIPLHPGADKFFKDMKVKK"
     gene            2287..3186
                     /locus_tag="VFL04_07680"
     CDS             2287..3186
                     /locus_tag="VFL04_07680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7839623.1"
                     /translation="MRFPIGIITALVLILAGIILAPAASGLQPKEGPPPTEARVGLGS
                     SLAGLPSSPLPRELGDRAQILCWHSFLGQSSIVTDFSLEELAKQLDALLALGYRFVSL
                     DDLLAGRIRGSMNLVATLDDGHRTVPAAFAKVFAPRGIVPAIMVYPSIIGNVPYAMDE
                     AQLRYLRDSGCLVGAHGYHHLFVNEELYRTDRASFDQEVYRAKARVEDLTKRQIYVYA
                     YPFGAYSEITKREVARAGYDFGLAVAPGFVYADSRLNDRYALPRIVVRRDGWEELYAF
                     LQRNAALSRGLEAAIKTTPGQGG"
     gene            3416..>5450
                     /locus_tag="VFL04_07685"
     CDS             3416..>5450
                     /locus_tag="VFL04_07685"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET7839624.1"
                     /translation="MRSLLSPLPALPLLGARSWHSLMDGARSPADLAEAAAGRGWNAL
                     ALADTNNLYALPELADAAAAVGIKAIASASIETAGISLRAYCLNRKGFSRLCGILSRV
                     LAVEVPRRRLRLPRELLLEEAADYDETDNLLLAERSAEEAGREGRFPLHGRGARRRDP
                     GPRGRNEARVTYDPLADLAEGGWEGLALSASSAEVLRRLAGRGLLYAGLPLGLPQVAT
                     SRLARELSLPILAEHSARFVEAADAELWRLARAIEERRSLAAIEREGREEPQGSLLVA
                     RGRAAEFLSAFPEAQANAASLAEEAEAAASFFASPPVFPAYQGMSDEESFRLLRSLCE
                     SGVGRRYGPGPERREDLGPRLERELRIIRDKGFSAYFLVVRDIVLRCPRTCGRGSAAS
                     SLVSYLLGLTHVDPLEHDLFFERFLNEGRTDPPDIDIDFPWDERPGVLASVFRDYEGR
                     AAMVADHCGFSDAGAIRESALALGMNEKELKAARLAYRIGRADELPEELRRLARMVRG
                     MPRYIGMHPGGIVVTPGPITDYTHVQPSPAGLPVLAWEKDGTERAGLVKIDILGNRSL
                     AVLRDCIELANAVRAPEEALAWDFPGAPEDAAARVLVEAGKSMGVFYIESPATRQLLL
                     KMGTVDYRRLVVASSIIRPAANKYISEYVRRLHGGSWRRFPAAVEKTLEETLGI"
BASE COUNT          841 a         1870 c         1850 g          889 t
ORIGIN      
        1 ggtgctcgag accccgatgg gcgtgccgaa gaccccctcg gtggtgtagt agaagtgctc
       61 ggtgaggctc tcgagggtca cgccgcggtg ggcgaagcgg ccagggaggt aggggccggc
      121 gaaggcgtag gctatgaagg ccatcgcgac gatgacgatc ggaagaccga ccacgcggcg
      181 ggccgcctcg aggacgagga ggacgccgat gacgccgacg atgatgtcca ggggcgtggc
      241 agtgccggcg cgctgggaga ggaccttggt ccagaggacc gtgacgtaga gcggcgaggc
      301 ggccccgagc agggcgagga cggcgtcgac cgggtggagc ttcttcctcg accacttctt
      361 gctcgtgggg tagaggaggt agatgaggca gaaggcgaag ccgaggtgga cggagcgctg
      421 gatcatggcg tcaaagacgc cgaacagggc cgtgtagatc tggaacagcg agaaggagat
      481 cgcgatggcc gcgacgatcc tggcgatgac gccggtcagg tcgcggtagt cggcctcctt
      541 gtcgaacttt ttcattatct cctgggcatc gacgggggcc tcttcctgca ggctcgttgt
      601 tgtgtcgctc atgcgagctc acctccctag tagtatgtgc gttctcgtct ttgccgagag
      661 aacgatgagc gtctccggcc tcgcccagtt ccggaacggg aggtaggccc cgttcgcgag
      721 aaggccgtgg ccgggcagca ctgacacgaa aatcggtatc ttctcgaatc tgcggtccat
      781 cgacagcacg aacctgccgt cctccaggcg gtagcctccc tcggcgtccg agggcatgcc
      841 cacgccctgg ttctcgtagc ggagctcgta gagccggagc gtggcccggc cccggcccga
      901 ggcctcgatg gcgaagcgct cgaccacctc gctgcggtgc accgagtgca cgaagacgtg
      961 gtcgaagcgg ccgtccggaa gctcgatggc ggcgaggagc ctgccgccag gctccgagac
     1021 gaggagctct ggcagcggga ccagacgcgg atgggcgagg acgaggaagg ccgccagccc
     1081 cggcaccgcc agggcgaggg cggcccacct cttcgctgcc atttccggcg ctacttcttg
     1141 accttcatat ccttgaagaa cttgtcagcg cccgggtgca gggggatcga catgccgtcc
     1201 agggaggtga ggagcttgat gtcggcgccc ttcttgtggg aggcggcgag gcgctcgccg
     1261 ttggcgtaca tcgtcttcag gaggtcgtag acgagttggg gatccatgcc ggccttgacc
     1321 gcgagcatgg acttgacgga gaccgtcatg acgtcggagg tgacgccctg gtaggtgttg
     1381 gcggggatct tctcgggcgt gtagaaggac cacttcttca tgagggtggc ggcgatggcg
     1441 ccgtcgatgg ggacgaggac gatcttcttg gacgcgccga ggtcctggat ggcggcggtg
     1501 ggggcgcccg cggtgttgaa ggccgcgtcg atgttgccgt ccttgaggtt gctggcggac
     1561 tcggcgaagg agaggtactg gaccttgatg tccttgtagc tgaggccggc ggcctcgagg
     1621 acctggcggg cgtggatctc gacgccggag ccggcggcgc ccacggagac gcgcttgccc
     1681 ctgaggtcgg cgacggactt gatgccggag tcggcgaggg ccacgagctg gacggtctcg
     1741 ttgtagaggc aggccaggcc gcggatgtcc ttgaggggct tgtccttgaa gagctcgatc
     1801 ccgttcaggg cgtagaaggc gccgtcgttc tggacgaaga tgacgtcgac ctcgcccttg
     1861 cccagcatcg ccacgttggc gatcgaggct ccggtgaccg tggcgctcgc gttcgcgccg
     1921 gggatgttct tgttccagat ctcggcgagg gccccgccga gggggaagta ggtgccggcc
     1981 gtgccgcccg tggcgatggt gatgaacttc tgctgaccga agcctaggga cgcggcgacg
     2041 agcaggcaga gggcgatcgc gatgagcttc ttgttcattg aaagcgcctc caattggttt
     2101 tttcggggag gccgccggcc cgcgctcgct gtgcgcctcc ttggcccaag cctcccctca
     2161 gctgagcagt atacggccac ttcgcgatgc gcgccacgcc tcttttttcg ccggggcgag
     2221 gaaaatccgg aggctccccc caacttgcac agctgtggcg ctttcgtgta caccgggagc
     2281 caccctatgc ggttccccat cggcatcatt accgcccttg tcctcatcct ggcggggatc
     2341 atcctcgccc ctgcggcctc tggcctccag cccaaggagg gcccgccgcc aaccgaggcc
     2401 cgggtcggcc tcggatcgag cctggccggc ctcccctcat cgcccctccc ccgggagctc
     2461 ggcgacaggg cccagatcct ctgctggcac agcttcctgg gccagtcctc gatcgtcacg
     2521 gatttctcgc tcgaggagtt ggccaagcag ctcgacgccc ttctggccct cggctacagg
     2581 ttcgtgagcc ttgacgatct cctcgctggc aggatcaggg gctcgatgaa cctcgtggcg
     2641 accctcgacg acggccaccg cacggtgccc gccgccttcg ccaaggtctt cgcgccgcgg
     2701 ggcatcgtcc ccgccatcat ggtctacccc tccatcatcg gcaacgtgcc ctacgcgatg
     2761 gacgaggccc agctccgcta cctcagggac tcgggctgcc tcgtcggggc ccacggctac
     2821 caccacctct tcgtcaacga ggagctgtac aggaccgacc gagcctcctt cgaccaggag
     2881 gtctaccgag ccaaggcgag ggtcgaggac ctgacgaagc ggcagatcta cgtgtacgcc
     2941 tatcccttcg gcgcctactc cgagatcacc aaacgcgagg tcgcccgggc gggctacgac
     3001 ttcggccttg cggtggcccc gggcttcgtc tacgcggact cgaggctcaa cgaccgctac
     3061 gccctgccgc gcatcgtcgt gaggagggat gggtgggagg agctctacgc cttcctccag
     3121 cgcaacgccg ctctgtcgcg gggtctggag gctgcaataa aaacgacccc cgggcaagga
     3181 ggctaacccg ggggccgtag gccgctcatc ctcgcgctgg ggagtgggtg ctcatcgcgc
     3241 gggaatctag tgccgtactc gagtcgtcct ctcgagtaca ggatgaatat atcaaaaaac
     3301 tccataagcg tcaagatctg gtaaagacaa aaaatcgata atcgctatgt tcgaattccg
     3361 cgatatcgct gcgccacttg cccaagactg ccgaagtact atactgatgt atagtatgcg
     3421 gtccctgctt tcaccccttc cggcccttcc gctcctggga gcccggtcct ggcactccct
     3481 catggacggg gccaggtctc ccgccgacct cgccgaggcg gcggccggcc gtggctggaa
     3541 cgccctcgcc ctcgccgaca cgaacaacct ctacgccctc cccgagctcg ccgatgccgc
     3601 cgcggccgtc ggcatcaagg ccatagcctc ggcctcgatc gagaccgcgg gcatctcgct
     3661 gcgcgcctat tgcctgaacc ggaagggctt ctcccggctc tgcgggatcc tgagccgggt
     3721 cctggcggtc gaggtcccca ggcggaggct gaggcttccg cgcgagctcc tcctggagga
     3781 ggccgccgac tacgatgaga ccgacaacct cctcctcgcc gagcgatcgg ccgaggaggc
     3841 cgggagggaa gggcgttttc ccctccacgg gcggggagcc aggaggcgcg atccggggcc
     3901 ccgcggccgg aacgaggccc gggtcaccta cgatccactc gccgacctcg ccgagggggg
     3961 atgggagggc ctggccctct ccgccagctc ggccgaggtc ctccgccgtc tcgccggccg
     4021 gggcctcctc tacgccggcc tccccctcgg cctgccccag gtcgcgacct cccggctggc
     4081 ccgcgagctc tccctgccga tcctcgccga gcacagcgcc cgtttcgtcg aggcggccga
     4141 cgccgagctc tggcgcctcg cccgggcgat agaggagcgg cggagcctcg ccgcgatcga
     4201 gagggagggc cgggaggagc cccagggctc cctccttgtg gcccgcggca gggcggccga
     4261 gttcctctcg gccttccccg aggcccaggc gaacgccgcc tccctggccg aggaggccga
     4321 ggccgccgcc tccttcttcg cgagcccccc cgtcttcccg gcctatcagg gcatgagcga
     4381 cgaggagtcc ttccgcctgc tgcgctccct ctgcgaatcc ggggtcggga gacgctacgg
     4441 gccggggccg gagcggcgcg aagacctcgg gccaaggctc gagcgcgagc tgcgcatcat
     4501 ccgcgacaag ggcttctcgg cctacttcct cgtcgtgcgc gacatcgtcc tccgctgccc
     4561 ccgcacctgc ggccgcggca gcgcggcctc gagcctcgtc tcctacctcc tcgggctcac
     4621 ccacgtcgat cccctcgagc acgacctctt cttcgagcgc ttcctcaacg aggggaggac
     4681 agacccgccc gacatcgaca tcgacttccc ctgggacgag cgccccggcg tcctcgcctc
     4741 ggtgttccgc gactacgagg gcagggcggc catggtcgcc gaccactgcg gcttctcgga
     4801 cgccggggcg atcagggagt cggccctcgc cctgggcatg aacgagaagg agctcaaggc
     4861 agcccgcctc gcctaccgca tcgggagggc cgacgagctg cccgaggagc tgcgccgcct
     4921 cgcccgcatg gtccgcggca tgccccgcta catcggcatg caccccgggg gcatcgtcgt
     4981 caccccggga cccatcaccg actataccca cgtccagccc tcgcccgccg gcctcccggt
     5041 cctcgcctgg gagaaggacg ggactgagcg ggccggcctc gtcaagatcg acatcctggg
     5101 caaccgctcc ctcgccgtcc tccgcgactg catcgagctc gccaacgccg tccgcgcgcc
     5161 cgaggaggcc ctcgcctggg acttccccgg ggcgcccgag gacgccgcgg cccgcgtcct
     5221 cgtcgaggcg gggaagagca tgggcgtctt ctacatcgag tcgcccgcca cccgccagct
     5281 cctgctcaag atggggacgg tggactaccg caggctcgtg gtcgcgagct cgatcatcag
     5341 accggcggcg aacaagtaca tcagcgagta cgtcaggcgg ctccacggcg ggtcctggag
     5401 gcgtttcccc gccgcggtcg agaagaccct ggaggagacc ctcggcatca
//
LOCUS       DASSQV010000439         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_102857, whole genome shotgun sequence.
ACCESSION   DASSQV010000439 DASSQV010000000
VERSION     DASSQV010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102857"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFL04_07690"
     CDS             <1..497
                     /locus_tag="VFL04_07690"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET7839625.1"
                     /translation="LIIEAVENPGKEGFKLTGQMGSVMQESAGIAYTFVRHAAAELYD
                     IGADYFEGRQIHLHIPEGATPKDGPSAGITMATALLSLVTGRKIRDRLAMTGELSLTG
                     QVLPIGGLREKTVAAKRNKIRDIIIPAGNEKDLDDIPEHVKKGISFHPAERMEDVIAI
                     AFPD"
     gene            576..2384
                     /locus_tag="VFL04_07695"
     CDS             576..2384
                     /locus_tag="VFL04_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7839626.1"
                     /translation="MKRLGLVLAGLSIALFAASAAPRVIVSHALALHGTPKYAAGFAN
                     FDYANPKAPKGGTFRLHSIGTYDNFNRFADRGVSAEGVDGYLFDTLMTSSADEIEVYY
                     GLVAEKVEYADDYSWIIFDLNPKARAQDGKPITSDDVVFTFNKFMTEGVPQFKKYYEG
                     VKAEALSPLQAKFTLANPDKALMISLASLNVLPKAYWSTRKLSEPLMEVPVGTGAYTV
                     SDYKIGQYVVYKRLGDYWGDRLPVNVGMNNFDYIRYDYYRDETVAFEAFKAGEYDLYQ
                     ESISKNWATMYTGKPFEAGWIVKEEIPHEIPPGLQSFVFNTQRPMFQDRRVREAIGYA
                     LDFEWLNKNLFYSQYKRSRSYFQNTDYAATGLPSKEELRILEPIRSKIPAEVFTKEYQ
                     PPVTDGSGNIRDQLRAAMALFKDAGWEVRDEKLVNVKTGQPMVFELLLYSPSMERVAV
                     PIQKNLQRMGITMNIRLVDTGQYTKRMRDRDYDLIDRGFDPMAYPSTDLLEIWDSRYI
                     DDTYNLAGVKDPAIDYLVKGIVANQDDEKTLLYWGRALDRVLTWNYFVMPMWDISKFR
                     IAYWNKFARPGVRPKYSLGTGSWWIDSAKEAKLPKR"
     gene            2400..>2621
                     /locus_tag="VFL04_07700"
     CDS             2400..>2621
                     /locus_tag="VFL04_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611577.1"
                     /note="with YejAEF is involved in resistance to microcin
                     C; Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin ABC transporter permease"
                     /protein_id="HET7839627.1"
                     /translation="MTRYIIRRLLLMIPTLWAIITLNFFIVQIAPGGPVDQAVASLSG
                     LGAGPAMEQISGAGKKEVGSTEGAQQGKEK"
BASE COUNT          524 a          902 c          795 g          400 t
ORIGIN      
        1 ccctcatcat cgaggctgtc gagaaccccg gcaaggaggg cttcaagctt acgggccaga
       61 tgggctcggt gatgcaggag tcggcgggca tcgcctacac cttcgtcagg cacgcggccg
      121 ccgagctcta cgacatcggg gctgactact tcgaggggag gcagatccac ctccacattc
      181 ccgagggcgc gaccccgaag gacgggccct cggcggggat cacgatggcc acggccctcc
      241 tctccctcgt cacggggagg aagatcaggg acaggctcgc catgacgggc gagctctcat
      301 tgaccggcca ggtcctcccg atcgggggcc tccgcgagaa gacggtggct gcgaagcgca
      361 acaagatacg cgacatcatc atccccgcgg ggaacgagaa ggacctggac gacatccccg
      421 agcacgtgaa gaaggggatc agcttccatc cggccgagcg catggaggac gtcatagcca
      481 tcgccttccc ggactagggc ccttcattga ccgggaagcg gggtttctct attatcgatc
      541 ttgtgcggcc cgctttcccg ggcagaggag ggaacatgaa acggctcggt ctggtcctcg
      601 cggggctctc catcgccctc ttcgcggcct cggcggcgcc cagggtgatc gtgtcccacg
      661 ccctcgccct ccacgggacg cccaagtacg cggcgggctt cgccaacttc gactacgcca
      721 acccgaaggc ccccaagggc gggaccttca ggctgcactc catcggcacc tacgacaact
      781 tcaaccgctt cgccgaccgg ggggtctcgg ccgagggggt cgacggctac ctcttcgaca
      841 ccctcatgac ctcctcggcc gacgagatcg aggtctacta cggcctcgtg gccgagaagg
      901 tcgagtacgc cgacgactac tcctggatca tcttcgacct gaaccccaag gcccgcgccc
      961 aggacggcaa gccgatcacc tccgacgatg tggtcttcac cttcaacaag ttcatgaccg
     1021 agggcgtgcc ccagttcaag aagtactatg agggcgtcaa ggccgaggcc ctgagccccc
     1081 tccaggcgaa gttcacgctc gcgaaccccg acaaggccct catgatctcc ctggcgtcgc
     1141 tcaacgtcct gcccaaggcc tactggtcga cgaggaagct ctccgagccc ctcatggagg
     1201 tgccggtggg gacgggggcc tatacggtgt ccgactacaa gatcggccag tacgtcgtgt
     1261 acaagcgcct cggcgactac tggggggaca ggctcccggt caacgtgggc atgaacaact
     1321 tcgactacat ccgctacgac tactaccgcg acgagacggt ggccttcgag gccttcaagg
     1381 ccggggagta cgacctctac caggagagca tctccaagaa ctgggccacc atgtacacgg
     1441 gcaagccctt cgaggccggc tggatcgtga aggaggagat cccccacgag atcccgccgg
     1501 gcctgcagtc cttcgtgttc aacacccagc gcccgatgtt ccaggaccgg agggtccgcg
     1561 aggccatcgg ctacgccctc gacttcgagt ggctcaacaa gaacctcttc tacagccagt
     1621 acaagcgctc gcggagctac ttccagaaca ccgactacgc ggcgaccggg ctgccgagca
     1681 aggaggagct caggatcctc gagcccatac gctccaagat cccggccgag gtcttcacca
     1741 aggagtacca gccccccgtg accgacggct cgggcaacat ccgcgaccag ctgcgcgccg
     1801 cgatggccct cttcaaggac gccggctggg aggtcaggga cgagaagctc gtcaacgtga
     1861 agaccgggca gccgatggtc ttcgagctcc tcctgtacag cccctcgatg gagagggtcg
     1921 ccgtgcccat ccagaagaac ctccagcgca tgggcatcac catgaacatc aggctcgtgg
     1981 acaccggcca gtacaccaag cgcatgcgcg accgcgacta cgacctcatc gaccggggct
     2041 tcgaccctat ggcctacccc agcaccgacc tcctcgagat ctgggactcg cgctacatcg
     2101 acgacaccta caacctcgcc ggggtcaagg acccggccat cgactacctg gtcaagggca
     2161 tcgtcgcgaa ccaggacgac gagaagaccc tcctgtactg gggcagggcc ctcgacaggg
     2221 tcctcacctg gaactacttc gtgatgccga tgtgggacat cagcaagttc cgcatcgcct
     2281 actggaacaa gttcgcccgc cccggcgtca ggcccaagta cagcctcggg acgggctcct
     2341 ggtggatcga cagcgccaag gaagcgaagc tgcccaagcg atgacgggat gagccacgca
     2401 tgacccgcta catcatccgc aggctcctcc tcatgatccc cacgctctgg gcgatcatca
     2461 ccctcaactt cttcatcgtc cagatcgccc ccggcgggcc ggtggaccag gccgtggcct
     2521 ccttgagcgg gctcggcgcc gggcccgcga tggagcagat ctccggggcg ggcaagaagg
     2581 aggtgggctc caccgagggt gcccagcagg gcaaagagaa g
//
LOCUS       DASSQV010000440         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1095621, whole genome shotgun sequence.
ACCESSION   DASSQV010000440 DASSQV010000000
VERSION     DASSQV010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095621"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2129
                     /gene="mgtA"
                     /locus_tag="VFL04_07705"
     CDS             <1..2129
                     /gene="mgtA"
                     /locus_tag="VFL04_07705"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HET7839628.1"
                     /translation="VEKAAEPAGAGAEPQAAAAAAAASSIFDARNLCFQGSTVISGSA
                     RGIAVQTGMRTFLGSVAADLAEPKGATDFDRGVKSFVSLMVRFMLVMVSITFLAVGIT
                     KGDWLQALLFGLSVAVGLTPEMLPMIVTVCLSKGAVFMSRKQVIVKRLKAIQNLGAMD
                     LLCTDKTGTITQDKVVLERHVDVTNRPSEDVLRYAWMNSHYQTGLRNLLDDSILSNED
                     LDVERSCRKIDEIPFDFARKRMSVIIEYEGDHVLICKGSVDAVFEVCDRYQVDDDVLP
                     LIDVLKQDILEEYARLSSQGFRVLAIAYREYEATREVFSAADESGLILLGYLAFFDPP
                     KDSAEKAIRSLRQHGVRVKVLTGDNELVTAKVCADVGLAEAKVMTGRELASLDEAGFA
                     QAVQDHDVFARLSPADKERIVRALRSAGHVVGFMGDGINDAAAMRASDVGISVDTAVD
                     VAKESADIILLEKNLLVLEDGILEGRRVFNNIIKYIKMGASSNFGNMFSMLGSSLILP
                     FLPMTPVQILLNNLLYDASQTGIPLDNVDPESVERPQSWDISFIRRFMIALGPISSIF
                     DYATFALMWWFFGCSAWAGASAGDRAQLEALFHTGWFVESLLTQSLVVHIIRTNKVPF
                     FQSRSSLQLGVATFLVMALAVILPYAPFAAFFGLVPLPPIYWAWIAGFLVAYSCLAHL
                     VKTWFYRRASKSRNVSAVSTAKGGTA"
     gene            2126..2920
                     /locus_tag="VFL04_07710"
     CDS             2126..2920
                     /locus_tag="VFL04_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7839629.1"
                     /translation="MKSLLEALEEGRLLELPESGKEEALELLAHLIEAIPDIGTKTDL
                     MKSVMERESQANTAIGRGVACPHCRTAAEGELLCAVGWSPQGIDYGAADGRKVHLVVM
                     YYVPDSERNGYLKEISGLAKAISSGGDTIESISSLPDIHTVREKLLDWVGLALNEAIP
                     DPKARMIKLEARQAIAALAAAAPPMMVASGARFLPFRLVTWQGGCIVLCNDPGLADTL
                     EKDGELGSKIAHAREFDLPEYRIAVLSESIFAANRKEYMGVAIRME"
BASE COUNT          481 a         1085 c          897 g          484 t
ORIGIN      
        1 ccgtcgagaa ggcggccgag ccggccggcg ccggggctga gccccaggcg gccgccgctg
       61 cggccgcggc ctcgtcgatc ttcgacgcga ggaacctctg cttccagggc agcaccgtca
      121 tctcgggctc ggcccggggc atcgcggtcc agacgggcat gcggaccttc ctcggcagcg
      181 tcgccgccga cctcgccgag ccgaagggcg ccaccgactt cgaccgcggg gtcaagtcct
      241 tcgtctccct catggtccgc ttcatgctcg tcatggtctc gatcaccttc ctcgccgtgg
      301 ggatcaccaa gggggactgg ctccaggccc tcctcttcgg gctctccgtg gcggtaggcc
      361 tcaccccgga gatgctcccg atgatcgtca cggtctgcct gtccaagggc gcggtcttca
      421 tgtccaggaa gcaggtgatc gtgaagcggc tcaaggcgat ccagaacctc ggggccatgg
      481 acctcctgtg caccgacaag accggcacga tcacccagga caaggtcgtc ctcgagcgcc
      541 acgtcgacgt gaccaaccgc cccagcgagg acgtcctgcg ctacgcctgg atgaacagcc
      601 actaccagac cggactgcgc aacctcctcg acgactcgat cctctcgaac gaggacctcg
      661 acgtcgagcg cagctgccgc aagatcgacg agatcccctt cgacttcgcg cgcaagcgca
      721 tgtcggtcat catcgagtac gagggcgacc acgtcctcat ctgcaagggc tcggtcgacg
      781 cggtcttcga ggtctgcgac cgctaccagg tcgacgacga tgtcctgccc ctcatcgacg
      841 tgctcaagca ggacatcctc gaggagtacg cccggctctc ctcccagggc ttccgcgtgc
      901 tcgcgatcgc ctaccgcgag tacgaggcca caagggaggt cttctcggcc gctgacgagt
      961 cggggctcat cctcctcggc tacctcgcct tcttcgaccc gcccaaggac tcggccgaga
     1021 aggccatccg ctcgctcagg cagcacgggg tccgcgtgaa ggtcctcacc ggcgacaacg
     1081 agctcgtcac cgccaaggtc tgcgccgacg tgggcctcgc cgaggcgaag gtgatgacgg
     1141 gccgcgagct ggcctcgctg gacgaggcgg gcttcgctca ggcggtgcag gaccacgacg
     1201 tcttcgcccg gctcagcccc gccgacaagg agcgcatcgt gcgcgccctc cgctcggccg
     1261 gccacgtcgt cggcttcatg ggcgacggga tcaacgacgc cgccgcgatg cgcgcctccg
     1321 acgtgggcat ctcggtggac acggccgtcg acgtggcgaa ggagtcggcc gacatcatcc
     1381 tcctcgagaa gaacctcctc gtcctcgagg acgggatcct cgaggggcgg cgggtcttca
     1441 acaacatcat caagtacatc aagatgggcg cgagctccaa cttcggcaac atgttctcga
     1501 tgctgggctc ctcgctcatc ctgcccttcc tcccgatgac ccccgttcaa atcctcctca
     1561 acaacctcct ctacgacgct tcccagaccg gcatccccct ggacaacgtg gacccggaga
     1621 gcgtcgagcg gccccagagc tgggacatct ccttcatccg acggttcatg atcgccctcg
     1681 ggcccatctc ctcgatcttc gactacgcga ccttcgccct catgtggtgg ttcttcgggt
     1741 gttcggcctg ggccggcgcg agcgcgggcg acagggccca gctcgaggcc ctgttccata
     1801 ccgggtggtt cgtcgagtcc ctcctcacgc agagtctcgt cgtccacatc atccggacga
     1861 acaaggtgcc cttcttccag agccggtcca gcctccagct cggggtggcg accttcctcg
     1921 tgatggccct ggcggtcatc ctgccctatg cccccttcgc cgccttcttc gggctcgtcc
     1981 ccctgccgcc gatctactgg gcctggattg ccggcttcct cgtggcctac tcctgcctcg
     2041 cccacctcgt gaagacctgg ttctaccgac gggcatccaa atcgcgcaac gtgtcagccg
     2101 tctcgacggc gaaaggcggg accgcatgaa aagcctgctc gaggccctcg aagagggccg
     2161 gctcctcgag ctgcccgaat ccggcaagga ggaggccctc gagctcctcg cccacctcat
     2221 cgaggccatc cccgacatcg gcaccaagac cgacctcatg aagagcgtga tggagcggga
     2281 gtcgcaggcc aacacggcca tcggccgggg agtggcctgc ccgcactgcc gcaccgcggc
     2341 cgagggcgag ctcctgtgcg ccgtcggctg gtccccccag ggcatcgact acggagcggc
     2401 cgacgggcgc aaggtccacc tcgtcgtgat gtactacgtg cccgactccg agcgcaacgg
     2461 ctacctcaag gagatctccg gcctcgccaa ggccatctcc tcgggcggcg acacgatcga
     2521 gagcatctcg agcctgcccg acatccacac cgtccgcgaa aagctcctcg actgggtggg
     2581 cctcgcgctc aacgaggcga tacccgatcc caaggcgcgg atgatcaagc tcgaggcgcg
     2641 gcaggccatc gccgccctcg ccgccgcggc gccgcccatg atggtggcct cgggcgcgcg
     2701 cttcctcccc ttccgcctcg tcacgtggca gggaggctgc atcgtgctgt gcaacgaccc
     2761 cgggctcgcc gacacgctcg aaaaggacgg cgaactcggc tccaagatcg ctcacgcccg
     2821 cgagttcgac ctccccgagt accgcatcgc ggtcctctcg gagtcgatct tcgccgcgaa
     2881 ccgcaaggaa tacatgggcg tggcgatccg gatggaatga tgtcgcctga cgtttcatga
     2941 ggcagcc
//
LOCUS       DASSQV010000441         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_974771, whole genome shotgun sequence.
ACCESSION   DASSQV010000441 DASSQV010000000
VERSION     DASSQV010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974771"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFL04_07715"
     CDS             complement(<1..336)
                     /locus_tag="VFL04_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET7839630.1"
                     /translation="MFKGLTHRAQRVLTILAQEEAKRTRAEQVLPEHILLAIVKEGDG
                     VGFKAMRSLKIDTLELRDELDRTIEHRRSGIVLGDLPLSRRARSLLEGAAEEANMAGS
                     DYIGTEHFIV"
     gene            complement(336..1886)
                     /locus_tag="VFL04_07720"
     CDS             complement(336..1886)
                     /locus_tag="VFL04_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839631.1"
                     /translation="MPWTIVHAVTCAQCGERTAAIFIRRSVSGSGPLGGEGPAQGGGS
                     DLALCEICARSRGITAAKGRLDIGIEELMRAAAVPASESGMPAACPSCGSSLAQLRRL
                     GRLGCPSCIEAFYPAIRRAFGPLPREASPRTAPSSAVAVERPPLAPSAPPPEAGPTSF
                     PFLASGGGHDSDVVLSSSVSLSRSIEGLPFPGGPGGGAAPSRSLVSHALEGLPRLRRW
                     TMRQLDPATRRCLSERGLVPRSYAADPEALLAASIDFPLYVLADEGEHLRLRARFPGL
                     ALEAGIGAVQELHDRLAARGLRFLWDPRIGWVCASIEDCGIGMSLALSLHLPALAMTG
                     LHERFLRSILREGVAVRGSYSEGESSAGAVYEAVIGPSSRLGLDGMVDGMRAIAEQAS
                     QAERRARAELASKARGELLDALGRAYGLALHAHRLGPGEGAALASTLRLGAILGLLRK
                     EADNETEAVGSSLVDRLGRCMECMGPASIAAESGLAEQRSGAEDEELRSEFLRSALKG
                     AMLLVGGH"
     gene            1935..2903
                     /locus_tag="VFL04_07725"
     CDS             1935..2903
                     /locus_tag="VFL04_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HET7839632.1"
                     /translation="MRPRLSTALLALLAASALAAGASAQSRPLVIAALKGPSGIGLVK
                     LFEKPPVLPDAGTVSPIAVGSADLMAAKVISGEYDMAVLPVNMAAKLYSSGIGIRLVA
                     VIGNGMVSFLTSDPSINSLADLRGKEIAVAGQGATPDYLFRRLLKGAGLDPDRDLRLS
                     YALPYPEAAMALAAGKISCAVLPEPFASLALASKPDLRPALDLGRLWTQATGEASYPM
                     TALVASSTLAAQRPDAVGALLAACSSSIDWVKANPKDAGALVEKLDLGLKAGIAEKAI
                     PRSNYTYLSALQARAGIEALLATFLEMAPASIGGKLPDAAFYASFK"
BASE COUNT          466 a         1038 c          970 g          438 t
ORIGIN      
        1 cacgatgaag tgctccgtcc cgatgtagtc ggagcccgcc atgttcgcct cttccgccgc
       61 gccctcgagg agggacctgg ccctcctcga aagcggaagg tcgccgagga cgatccccga
      121 ccgccggtgc tcaatggtgc ggtcgagctc gtcgcgcagc tcgagcgtat ctatcttcag
      181 gctgcgcatc gccttgaagc cgacgccgtc gccttccttg acgatcgcaa gcaggatgtg
      241 ctcgggcagg acctgctcgg cccgcgtgcg cttcgcctct tcctgggcga gtatcgtaag
      301 gacgcgctgg gccctgtggg tcaatccctt gaacatcaat ggcctcccac taataacata
      361 gcgcctttga gggccgagcg gaggaattcc gagcgaagtt cctcgtcttc ggccccgctc
      421 ctctgttcgg caagccctga ctcggcggcg atgctggccg gtcccatgca ttccatgcat
      481 cgaccgagtc ggtcgacaag gctcgacccg acggcctccg tctcattgtc ggcctctttc
      541 ctaaggaggc caagaatcgc cccgagcctc agggtggagg ccaaggccgc cccctcgccg
      601 gggccgaggc gatgcgcgtg aagggcgagg ccgtaggccc gtcccaaggc atcgaggagc
      661 tcgcctcggg ccttcgaggc gagctcggcc ctcgccctcc tctcggcctg acttgcctgc
      721 tcggcgatgg ccctcatccc gtccaccatg ccatcaaggc ccagacgcga ggaggggccg
      781 atgacagcct catagaccgc gcccgccgag ctctccccct cggaatagct tcccctcacc
      841 gcgacgcctt ccctcaagat actgcggagg aagcgctcgt ggaggccagt catcgccagg
      901 gccggcaggt ggaggctcag cgcaagactc atgccgatcc cgcagtcctc gatgctcgcg
      961 cagacccagc ctatcctcgg atcccagaga aagcggagcc ctcgggcggc gagccggtcg
     1021 tggagctcct gcactgcccc gatgcccgct tcaagggcga ggccggggaa gcgtgccctg
     1081 agccgcagat gctcgccctc gtcggccagg acgtacagcg ggaagtcgat actcgcggcg
     1141 aggagggctt cagggtcggc ggcataggag cggggcacaa ggccccgctc ggacagacag
     1201 cggcgcgtgg cgggatcgag ctggcgcatc gtccagcgcc tcagccgggg gaggccctcg
     1261 agcgcatggg agacgaggga gcgcgagggg gcggccccgc ctccgggccc tccggggaag
     1321 gggaggccct cgatgctgcg ggacaggctg accgagctcg agaggacgac gtcggagtcg
     1381 tggccgcctc cggatgcgag gaagggaaag ctcgttgggc ccgcctcggg gggcggggcg
     1441 gagggggcca agggcggccg ctccaccgcc acagccgagc tcggcgcggt tcgcgggctg
     1501 gcctcgcgag ggaggggccc gaaggcacgg cggatggccg ggtagaaggc ctcgatgcag
     1561 gaggggcagc cgagccttcc aagccgacgc agctgggcga ggctggagcc gcaggaggga
     1621 caggcggctg gcatgcccga ctcgctcgcg gggactgccg cggcgcgcat aagctcctct
     1681 atgccgatgt ccagccggcc cttggccgcc gtgatgcctc gggaacgggc gcagatttcg
     1741 cacaacgcga ggtctgagcc cccgccctgg gccgggccct cgccgccgag cggccccgag
     1801 cccgagacgc tccgacgtat gaagatggct gcggtccgct cgccgcactg cgcgcaggtc
     1861 acggcatgga ctatagtcca gggcatggaa aaagtatagc gttgcgtata tattcgcagc
     1921 gaaggagccc tccgatgaga ccacgcctca gcaccgcact gctcgccctg ctcgccgcct
     1981 cagccctcgc cgcaggggcc tcggcccaga gccggccctt ggtcatcgcc gccctcaagg
     2041 gcccctcggg catcggcctc gtcaagctct tcgagaagcc tccggtcctc cccgatgccg
     2101 ggacggtgag ccccatcgcg gtcggcagcg ccgacctcat ggccgcaaag gtgatctcgg
     2161 gcgagtacga catggccgtc ctgccggtga acatggcggc gaagctctac agttcgggaa
     2221 tagggatcag gctcgtcgcc gtcatcggaa acggcatggt ctccttcctc acctcggatc
     2281 cgtcgatcaa cagccttgcc gacctccgcg gcaaggagat agcggtggcg ggtcaaggcg
     2341 ccacccccga ttacctcttc cgccgcctgc tcaagggcgc ggggctcgat cccgaccgcg
     2401 acctcaggct ctcctacgcc ctcccctatc ctgaggccgc gatggccctc gccgcgggca
     2461 agatctcctg cgccgtcctg cccgagccct tcgccagcct cgccctcgcc tcgaagcccg
     2521 atctcaggcc agccctcgac ctcggccggc tttggaccca ggccaccggt gaggccagct
     2581 atccgatgac agccctcgtc gcgagctcga ccctcgccgc gcagaggccg gacgccgtgg
     2641 gggcccttct tgccgcatgc agctcctcga tagactgggt caaggccaac ccgaaggacg
     2701 cgggcgccct cgtcgagaag ctggaccttg ggcttaaagc gggcattgcg gaaaaggcca
     2761 tcccgcgcag caactacacc tatctcagcg ccctccaggc gagggccggc attgaagccc
     2821 tcctcgccac cttcctcgag atggctcccg cctcgatcgg cgggaagctg cccgatgcgg
     2881 ctttctacgc ctcgttcaag taaggagccg gg
//
LOCUS       DASSQV010000442         6528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2217952, whole genome shotgun sequence.
ACCESSION   DASSQV010000442 DASSQV010000000
VERSION     DASSQV010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2217952"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFL04_07730"
     CDS             complement(<1..838)
                     /locus_tag="VFL04_07730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7839633.1"
                     /translation="MEAKEVFELSDGVVEQIVFAMEDQEAKAVLDLETGEVLPAEGHS
                     GDRYATAPTWSSREGFRLMEDFLATVRLPSARRELGLALGRGRGVFKAFKAVLAAYPD
                     LERAFRDFKHRAMRRSISAWYDDLREAKGLERLGPEPEETEDLIVSDLDIRVEGLGAC
                     GRILEPLIRAAEEEALDFLPAAIASFEVARLRGELAGSADGFCAYVDDGEGGALGAAA
                     AVRLVARDRGIGRIVFLSVREGFRRMGLGRALLRAIAAKLSAEGATFLILDSALLPGE
                     YGE"
     gene            968..1813
                     /gene="thyX"
                     /locus_tag="VFL04_07735"
     CDS             968..1813
                     /gene="thyX"
                     /locus_tag="VFL04_07735"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET7839634.1"
                     /translation="MAHCVVPAAEEILDRIHPVLDKGFVRLVDYLGGDERIAQAARVS
                     YAGGTRSYREDSALIDHLLRNEHTSPFEQVVLTFHIKLPIFVARQWIRHRTARVNEVS
                     GRYSVMKDEFYLPRREDVALQSSDNKQGRAPGAAPEEVVDSALGRLADGQKAAYEDYS
                     ALVEAGLARELARIDLPLSLYTEWYWQIDLHNLFRFIALRLDPHAQLEIRAYAAVILE
                     IARAVAPAATSSFENHRLNGVSFSERELKELRLRLAGGGGEVTRLSGKELERFEEKLR
                     SGRQL"
     gene            complement(1823..2872)
                     /locus_tag="VFL04_07740"
     CDS             complement(1823..2872)
                     /locus_tag="VFL04_07740"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HET7839635.1"
                     /translation="MRRKGFEPAPEAVVDRGGFEYGRFGTPFGRANMLDVERPYLYPL
                     PRFVKRLRLKEWRDFHFGDERWFFFTAMYDAKIASLAMFHAYDRETKRHFEVQRIVPG
                     SAFAFGESLSGSRIAYRGSRTFIELLCEFDKGFAQVAVLRNSRDRRKRFSGRFRFTYG
                     EKTSATQAVCLPLGMNRAMYSTKTCMPLEGEFSADGQTHSLGGIAAMGVFDDHKGYYP
                     WRMHFDWVSGFGLDHRGRRIGFNLTDNQVRDQVKFNENCLWINNRTWPLPPIRVTRPH
                     GHDGEWVIQDTEGMVDLIFVPEVRNDIQFRLGLLDTDYHGPFGSFRGVIKNGEGEKVQ
                     AESLYGAGEQKYLRA"
     gene            complement(2869..3768)
                     /locus_tag="VFL04_07745"
     CDS             complement(2869..3768)
                     /locus_tag="VFL04_07745"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7839636.1"
                     /translation="MDPAKDIVGFVGTGVMGASMAGHLLKAGYRVHVTTRTKAKAKAL
                     IDAGAVWEEDPAVLAPKCTAIISMVGYPADVEQVYFGPAGLIEKAKPGSLLIDTTTSR
                     PDLAKRIYDAAAKKGLHALDAPVSGGDVGAKNATLTIMVGGDEAAFTAAKPLLDIMGK
                     TVILQGPAGAGQHTKMANQIVIAGNLICAVEAIMYARGAGLEPRRVLQSIGSGSAGSW
                     QLNTMVPRMLDGDFAPGFYVKHFLKDLRIALESAKDMKIELPLLGLAERLFAVMQEEK
                     FGDRGTQALYLLYEQGKVSGALG"
     gene            complement(3824..4312)
                     /locus_tag="VFL04_07750"
     CDS             complement(3824..4312)
                     /locus_tag="VFL04_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HET7839637.1"
                     /translation="MTIDTVRLSWTEGCNIVVGQSHFIKTVEDIAEIMAGSVPGSKWG
                     LAFNEASGPCLVRTEGSDPALVADAAGCARAAGAGHSFYLVLGPGTYPINVLDRIKAC
                     PEVCRIFCATANPLELIVARTEQGAGILGVVDGSSPKGVEGEADKADRIALLRRFGYK
                     RG"
     gene            complement(4365..4991)
                     /locus_tag="VFL04_07755"
     CDS             complement(4365..4991)
                     /locus_tag="VFL04_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET7839638.1"
                     /translation="MKLIRCLIFDLGGVITLPQKMEKVDEMIEAIGLRLPRASFQSAY
                     FAQRAAYDRGDISVEEYWRRVGRDLGAEPDGAAVDRLKKADIQSWFNINEGVVELLRE
                     ARRKVGKLVLLSNLPVEGAKHLRSSYDWLGLFDELVLSCELRRIKPESEIYELCLERS
                     GARAGDSLFVDDSPANTDAARALGMSAISFEGLEELKTGLAGGYELAK"
     gene            complement(4988..5575)
                     /locus_tag="VFL04_07760"
     CDS             complement(4988..5575)
                     /locus_tag="VFL04_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839639.1"
                     /translation="MAIVDELALAQRTVARKDFLGGLLRRIGLCAILFVPLVGLFSIV
                     NPRTSLAGTATYVGYLLVLIFALSAAFDGLEYLLTRARISRPAPLGQGERICYAVSFE
                     PEGRSFRKRHGMLLQGPEGLVFWDAQARRAQILGEGIEAFESERYYLRDPYSLRRRQM
                     RPLIEMAAQGGKRVFDVLAPEPALASLRKESGDGR"
     gene            complement(5559..5792)
                     /locus_tag="VFL04_07765"
     CDS             complement(5559..5792)
                     /locus_tag="VFL04_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839640.1"
                     /translation="MRIAGLNDLIEATLIDSMLGEQGIPHLVVSHSDAAYDGVFTVTR
                     GWGHLEAAEEDRAAVLELLSALKLAKAEGGDRG"
     gene            6032..>6528
                     /locus_tag="VFL04_07770"
     CDS             6032..>6528
                     /locus_tag="VFL04_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7839641.1"
                     /translation="MDREEERRNELLDRGMVLFFMEGISAFTMEGLAARLGVSKRTLY
                     KYYPKKELFVDAAAQRMLDKVRGQIEAHLATESEFPQRLKGFFMIVQTTLQPAARLLV
                     RDIMTAAPWLWEKVSRYRHDKIFSLLEGLLDEGRAKGYIRSDIEPRLIAPLFIGMIEQ
                     VAQPA"
BASE COUNT         1075 a         2183 c         2155 g         1115 t
ORIGIN      
        1 gctccccgta ctcgccgggg agcagggccg agtcgaggat aaggaaggtg gccccctcgg
       61 cgctcagctt tgcggcgatg gcccggagga gggccctgcc aagacccatg cgccggaacc
      121 cctccctcac cgaaaggaag acgatcctgc cgatgcccct gtccctcgcc acgaggcgga
      181 ccgccgccgc cgctccgagc gcacccccct ccccgtcgtc gacataggcg cagaagccgt
      241 cggccgagcc ggccagctct ccccggagcc tggccacctc gaaggaggcg atggccgccg
      301 ggaggaagtc gagggcctcc tcctcggccg ccctgatcag gggctcgagg atgcggccgc
      361 aggcgcccag gccctcgacc ctgatgtcca ggtcgctgac gatcaggtcc tcggtctcct
      421 ccggctccgg ccccagccgc tcgaggccct tggcttcgcg caggtcgtcg taccaggccg
      481 agatggaccg ccgcatggcc ctgtgcttga agtccctgaa cgcgcgctcg aggtcggggt
      541 aggccgcgag gacggccttg aaggccttga acacccctcg cccccgcccg agggccaggc
      601 cgagctccct cctcgcggat ggcaggcgca ccgtggccag gaagtcctcc atgagccgga
      661 agccctcccg cgatgaccag gttggtgcgg tcgcgtagcg gtctccgctg tgcccttcgg
      721 ccggcaggac ttcgccggtc tcgaggtcga ggactgcctt cgcttcctgg tcctccatcg
      781 cgaagacgat ctgctcgacc actccatcgc ttagctcgaa cacctctttg gcctccacca
      841 accgaggtat tcttggtgga ccggggtcgg atcgtcaagc gaaatgggtg tcacaatgaa
      901 tgcgctcggg gtcggcggcg ctgacgccga cggggcctcc gggctatact cgcgcccgga
      961 ggcatgcatg gcccactgtg tcgtccccgc ggccgaggag atcctcgacc gcatccatcc
     1021 cgtcctcgac aagggattcg tcaggctcgt cgactacctg ggcggggatg agcgcatcgc
     1081 ccaggccgcg cgggtttcct acgcgggcgg cacgaggagc taccgtgagg actcggccct
     1141 catcgaccac ctcctccgga acgagcacac ctcgcccttc gagcaggtgg tcctcacctt
     1201 ccacatcaag ctgcccatct tcgtcgccag gcagtggatc cgccaccgca ccgcgagggt
     1261 caacgaggtc tcgggccgct attccgtcat gaaggacgag ttctacctgc cgaggcgcga
     1321 ggacgtagcg ctccagagct cggacaacaa gcagggccgg gctcccggcg ccgcgcccga
     1381 ggaggtcgtc gactcggccc tcggccggct cgcggatggc cagaaggcgg cttacgagga
     1441 ctattcggcc cttgtcgagg ccgggctcgc gcgcgagctc gcccgcatcg acctccccct
     1501 gagcctctac acggaatggt actggcagat cgacctgcac aacctcttcc gcttcatcgc
     1561 cctccgcctc gacccccacg cccagctgga gatcagggcc tatgccgcgg tcatcctcga
     1621 gatagcgcgg gctgtcgcgc ccgcggcgac ctcgagcttc gagaaccacc ggctcaatgg
     1681 ggtcagcttc tccgaacgcg agcttaagga gctgaggctg cgcctggccg gcggaggcgg
     1741 ggaggtcacg aggctttcgg ggaaggagct ggagcgcttc gaggagaagc tcaggtcggg
     1801 ccggcagctc taggaagtca atctaggcgc gcagatactt ctgttcgccc gcgccgtaga
     1861 gcgactcggc ctggaccttc tcgccctcgc cgttcttgat cacgccccgg aacgagccga
     1921 aggggccatg gtagtcggtg tcgagaaggc cgaggcggaa ctggatgtcg ttcctgacct
     1981 cggggacgaa gatgaggtcg accatgccct cggtgtcctg gatgacccac tcgccgtcgt
     2041 ggccgtgggg ccgcgtcacc cggatgggcg gaagcggcca ggtcctgttg ttgatccaga
     2101 ggcagttctc gttgaacttg acctggtccc tgacctggtt gtcggtgagg ttgaagccga
     2161 tcctcctgcc cctgtggtcg aggccgaagc ccgagaccca gtcgaagtgc atgcgccagg
     2221 gatagtagcc cttgtggtcg tcgaagaccc ccattgcggc gatgcccccg aggctgtggg
     2281 tctggccgtc ggcgctgaac tcgccctcga ggggcatgca ggtcttggtc gagtacatcg
     2341 cccggttcat gccaaggggg aggcagacgg cctgggtggc cgaggtcttc tccccgtagg
     2401 tgaagcggaa cctgcccgaa aagcgcttgc ggcggtcgcg gctgttgcgg aggaccgcca
     2461 cctgggcaaa gcccttgtcg aactcgcaga gcagctcgat gaaggtccgt gagccgcggt
     2521 aggcgatgcg cgagcccgaa agggactccc cgaaggcgaa ggcgctcccg ggcacgatcc
     2581 gctggacctc gaagtggcgc ttggtctcgc ggtcgtaggc gtggaacatc gcgaggctcg
     2641 cgatcttggc gtcgtacatc gcggtgaaga agaaccagcg ctcgtcgccg aaatggaagt
     2701 ccctccactc cttgagcctg agccgcttca cgaagcgggg gaggggatag aggtaggggc
     2761 gctcgacgtc gagcatgttc gctcggccga agggcgtgcc gaagcgcccg tactcgaacc
     2821 cgcccctgtc gacgactgcc tcgggggcgg gctcgaagcc cttgcgtctc aacccagggc
     2881 cccgctcacc ttcccctgct cgtagagcag gtagagggcc tgggtacccc tgtccccgaa
     2941 cttctcctcc tgcatgacgg cgaagagccg ctcggcgagg ccgaggaggg gaagctcgat
     3001 cttcatgtcc tttgccgact cgagggcgat cctgaggtcc ttgaggaagt gcttgacgta
     3061 gaagccggga gcgaaatcgc cgtccagcat gcgggggacc atggtgttga gctgccagct
     3121 gcccgccgag cccgagccga tgctctggag gaccctgcgg ggctcgaggc ctgcgcccct
     3181 ggcgtacatg atcgcctcga cggcgcagat gaggttgccc gcgatcacga tctggttcgc
     3241 catcttggtg tgctggccgg ctccggccgg gccctggagg atcacggtct tgcccatgat
     3301 gtcgagcagg ggcttggcgg ccgtaaaggc cgcctcgtcg cctccgacca tgatggtgag
     3361 ggtggcgttc ttggccccga cgtcgccgcc cgagacaggg gcatccaagg cgtggaggcc
     3421 cttcttcgcg gccgcgtcgt agatccgctt cgcgaggtcg ggccgggagg tcgtcgtgtc
     3481 gatgaggagg ctgcccggct tcgccttctc gatgaggccg gcggggccga aatagacctg
     3541 ttcgacgtcg gcggggtagc cgaccatgct gatgatggcc gtgcacttgg gcgcgagaac
     3601 ggcggggtcc tcctcccata cggcgcccgc gtcgatgagg gccttggctt tggccttcgt
     3661 cctcgtggtg acgtggacgc ggtagccggc cttgaggaga tggccggcca tgctggcgcc
     3721 catgacgccc gtgccgacaa agccgacgat atctttggct ggatccatta tgtgcctccg
     3781 ggttgtagaa agtgccttac tgtacccttc gccgtcggaa ggatcaaccg cgcttgtagc
     3841 cgaagcgccg aagcagcgcg atgcggtcgg ccttgtcggc ctctccctcg acccccttgg
     3901 gggaggagcc gtcgacgacg ccgaggatcc ccgcgccctg ctcggtcctc gccacgatga
     3961 gctccagggg gttcgccgtc gcgcagaaga tgcggcagac ctcggggcag gccttgatcc
     4021 tgtcgagcac gttgatgggg taggtccccg gcccgagcac gagatagaag ctgtggccgg
     4081 cgccggccgc gcgggcgcag cccgccgcgt cggcgaccag ggccgggtcg ctcccttccg
     4141 tgcgcaccag acagggtccc gaggcctcgt tgaaggcaag gccccacttt gagccgggca
     4201 cgctgccggc catgatctcg gcgatgtcct cgaccgtctt gatgaagtgg ctctggccca
     4261 cgacgatgtt gcagccctcg gtccaggaga ggcgcactgt atcaattgtc atattcatga
     4321 cctccggttt cggattcttg cgtgcccgcg ggcccacgct gcctttattt ggccagctcg
     4381 tagcccccag caaggcccgt cttgagctcc tcgagcccct cgaaactgat ggcgctcatc
     4441 cccaaggccc gggccgcgtc ggtgttcgcc ggggagtcgt cgacgaaaag gctgtccccg
     4501 gcccgggccc cgctgcgctc caggcagagc tcgtagatct cgctctcggg cttgatcctc
     4561 cggagctcgc aggagagcac aagctcgtcg aaaagcccga gccaatcgta gctcgacctc
     4621 agatgcttgg ccccctccac ggggaggttg gagaggagga cgagcttgcc gaccttgcgt
     4681 cgcgcctcgc gcaggagctc gaccacaccc tcgttgatgt tgaaccagct ctggatgtcc
     4741 gctttcttga gccgatcgac cgcggcaccg tcgggctcgg cgccgaggtc cctgccgacc
     4801 cgcctccagt actcctcgac cgagatgtcg ccgcggtcgt aggcggcgcg ttgggcgaaa
     4861 taggcggact ggaagctcgc ccgcggcagc ctgagcccta tcgcctcgat catctcgtcg
     4921 accttctcca tcttctgcgg cagggtgatc accccgccaa ggtcgaatat cagacagcgt
     4981 atcagcttca tcggccatcc ccgctttctt ttctcaggct cgccagggcg ggctccggcg
     5041 ccaggacatc gaagaccctt ttcccgcctt gggcggccat ctcgatgagg ggccgcatct
     5101 gccttctgcg cagcgaatag gggtcgcgga ggtagtatct ctcggactcg aaggcctcga
     5161 tcccctcgcc gaggatctgg gcgcggcgcg cttgggcgtc ccagaagacc aagccctcgg
     5221 gcccctgcag gagcatgcca tggcgcttgc ggaacgagcg gccctcgggc tcgaagctca
     5281 ccgcgtaaca tatccgctcg ccctggccca ggggggctgg tctggagatc cgcgcgcgcg
     5341 tgaggaggta ctcgagccca tcgaaggcgg cgctcaaggc gaatatgagg acaaggaggt
     5401 agccgacata ggtcgcggtc ccggcaagcg aggtcctcgg attgacgatc gaaaagagcc
     5461 cgaccaatgg gacgaaaagg atcgcgcaga gtccgattcg ccggaggagg cctcccagga
     5521 aatccttcct cgccacggtg cgctgggcca gggccagctc atccacgatc gccaccctcg
     5581 gccttcgcga gcttcagggc ggagaggagc tccaataccg cggccctatc ctcctcggcc
     5641 gcctcgaggt ggccccagcc cctggtgacg gtgaagaccc catcgtaggc ggcatcggaa
     5701 tgcgacacca cgaggtgggg aatgccctgc tcgccgagca tcgagtcgat cagcgtcgcc
     5761 tcgatgaggt cgttcaggcc tgcgatcctc acaaaaggtc ccaaccttcc tcccttgcgc
     5821 ggcccggctg cgctcctggt tcgtgatgcc cggaacgggc ctgggccgcc ctcgagtata
     5881 tgaccagacg acgaggagga caatcgagcc agcatggccc ggtttggcct tgacggagac
     5941 cctagggctc catattatac acttagtata cgaatggaat gcaattcgta tacttcgact
     6001 acggcccggt ttcttccggc aggggacgaa gatggacaga gaagaggaaa ggcgcaatga
     6061 gctgctcgat cgcgggatgg tgctgttctt catggaaggc atcagcgcct tcacgatgga
     6121 gggtctggcc gcccggctcg gggtaagcaa acggaccctc tacaagtatt atcccaaaaa
     6181 ggaattattc gtagacgcag cggcgcagcg gatgctcgac aaggtccgcg gccagatcga
     6241 ggcccacctc gccaccgaga gcgagttccc gcagcggctc aagggcttct tcatgatcgt
     6301 ccagaccacc ctgcagccgg cggccaggct cctcgtccgc gacataatga ccgccgcccc
     6361 ctggctctgg gagaaggtct cgcgataccg acacgacaag atattctccc tgctcgaagg
     6421 cctcctcgac gagggccggg ccaagggcta catcaggtcg gacatcgagc cgcgtctgat
     6481 cgcgccgctc ttcatcggca tgatcgagca ggtggcccag cccgcctt
//
LOCUS       DASSQV010000443         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1470352, whole genome shotgun sequence.
ACCESSION   DASSQV010000443 DASSQV010000000
VERSION     DASSQV010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470352"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VFL04_07775"
     CDS             complement(<1..1146)
                     /locus_tag="VFL04_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7839642.1"
                     /translation="MNAAEQARIPAEPWADERRQLLADKAALERRVAELERKISLQPS
                     SGLPTHFRLELELDETIERMRRAKDASFTVLIIQLGENYTVVRKTLKPSVSEWILYQT
                     GCRIESLLGPDDRVFHTRESEFVLILPGLLGKALAAFLRRLMAKLGEPHIFSGFNVVI
                     QAATGAAYWPHHGLERSVLLHNADIAAGSATEQRKHFVLFKPELLRCAVEKVELQNSI
                     IKAIERAALDSIGDQFVLYFQPKILASALEGNILKVERVEAEALIRWVHPEKGMIMPS
                     TFIPLAEETGLILPLGKWLIYQCIRRLDAWTRKAGGEDLGISINLSARQFRSEEGPEV
                     LASALASTGLAPGRVTIELTETSLFEDPIATASMLKRFSDLGIRVSVD"
     gene            complement(1312..>2215)
                     /locus_tag="VFL04_07780"
     CDS             complement(1312..>2215)
                     /locus_tag="VFL04_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7839643.1"
                     /translation="VKDISDRIASERALWNTNMELAEANASLKRTQMMMVQHEKLASI
                     GQLAAGIAHEINNPLGFLKSNHGVLAGFLRSIRAAWEEASQEEPSRLATIARRHDLAY
                     VFSEIDSLVKDSDEGYLRIMEIVKNLKNFARIDAEATIGPFDLNKGIESSLVVARNEV
                     KYVAEVRLELGEIPMIQAAGGEINQVMLNLIINAAQAIESQKRGAPGTIAIATRLEEG
                     RVVCTVADDGPGVPDELRLSIFDPFFTTKEPGKGTGLGLSISYDIIVNKHRGDLTVEP
                     SPLGGAMFRIELPVSPSADGASPN"
BASE COUNT          344 a          667 c          776 g          428 t
ORIGIN      
        1 gtccaccgac accctgatgc cgaggtccga gaagcgcttg agcatggaag ccgtggcgat
       61 cgggtcctcg aagaggctgg tctcggtcag ctctatcgtg acgcggccag gcgcgagccc
      121 cgtcgaggcc agggccgaag ccaagacctc tggcccctcc tcgctcctga actgccgggc
      181 cgagaggttg atggaaatgc ccagatcctc tcccccagcc ttgcgcgtcc aggcatcgag
      241 cctgcggatg cactggtaga tgagccattt gccgaggggg aggatgaggc cggtctcctc
      301 ggcgaggggg atgaaggtcg agggcatgat catgcccttc tcgggatgga cccagcggat
      361 cagggcctcg gcctcgacgc gctcgacctt gaggatgttg ccctcgaggg ccgaggcgag
      421 gatcttgggc tggaagtaga ggacgaactg gtccccgatg ctgtcgaggg ctgccctctc
      481 gatcgccttg atgatggagt tctggagctc gactttctcg actgcgcagc gcaggagctc
      541 gggcttgaag aggacgaagt gtttccgctg ttcggtggcg gagccagcgg ctatgtcggc
      601 gttgtgcagg aggactgagc gctcaaggcc gtggtgcggc cagtaggcgg ctcccgtggc
      661 cgcctggatc acgacgttga agccggagaa gatgtggggc tccccgagct tcgccatgag
      721 cctccggagg aaggccgcca gggccttgcc caggaggccc ggcaggatga ggacgaactc
      781 gctctcgcgg gtgtggaaga ccctgtcgtc ggggccgagg aggctctcga tgcggcagcc
      841 ggtctggtag aggatccact cgctcacgct cggcttgagg gtcttgcgca cgaccgtgta
      901 gttctcgccg agctggatga tgaggacggt gaagctggcg tccttcgcgc ggcgcatcct
      961 ctcgatggtc tcgtcgagct cgagctcgag cctgaagtgg gtggggagtc ccgagctggg
     1021 ctggagggat atcttgcgct cgagctcggc gacccggcgc tcgagggcgg ccttgtctgc
     1081 gaggagctgc ctgcgttcat cggcccatgg ctcggccggg atccttgctt gctctgcggc
     1141 gttcacgttc ggggcgcacc tgtcttcgtt gtctgccggc agaaacgctt cacaagcgga
     1201 tcggccacgc gcagtctagc tagaactatc ggtatcaggc cgcccgaagc ctagcgtttt
     1261 ctttgacgat tgtacatatt ccccagacag ggctcgccgc ttcccgcggc ctcagttcgg
     1321 cgatgccccg tctgcgctcg gggagaccgg cagctcgatc ctgaacatgg ccccgccgag
     1381 gggggagggc tcgacggtca gatcgccccg gtgtttgttg acgatgatgt cgtaggagat
     1441 cgaaagcccg agacccgtac ccttgcccgg ctccttggta gtgaagaagg ggtcgaagat
     1501 gctgaggcgg agctcatcgg gcacccctgg cccatcgtcg gccacggtgc agaccacgcg
     1561 gccctcctcg agccgggtag cgatcgcgat tgtccccggc gcgccgcgct tctgcgattc
     1621 gatcgcctgg gcggcgttaa tgatgaggtt cagcatcacc tggttgatct ctccgcccgc
     1681 ggcctggatc atcgggatct cgccgagctc gagcctgacc tcggccacgt acttgacctc
     1741 gttgcgcgcg accacgaggc tgctctcgat gcccttgttg aggtcgaagg ggccgatggt
     1801 ggcctcggcg tcgatccggg cgaagttctt gaggttcttg acgatctcca tgatccggag
     1861 atagccctca tcggaatcct tcaccaggga atcgatctcc gagaagacat aggccaggtc
     1921 gtggcgccgc gcgatcgtgg ccaggcggga gggctcctcc tgcgaggcct cctcccatgc
     1981 ggccctgatg gagcgcagga agcccgcgag gaccccatgg ttgctcttga ggaagccgag
     2041 ggggttgttg atctcgtggg ctatgccggc ggcgagctgg ccgatcgagg cgagcttctc
     2101 gtgctggacc atcatcatct gggtgcgctt gaggctggcg ttggcctcgg cgagctccat
     2161 gttggtgttc cagagggcgc gctcggatgc gatccgatcc gagatgtcct tgacg
//
LOCUS       DASSQV010000444         4301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_600636, whole genome shotgun sequence.
ACCESSION   DASSQV010000444 DASSQV010000000
VERSION     DASSQV010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600636"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VFL04_07785"
     CDS             complement(<1..1162)
                     /locus_tag="VFL04_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HET7839644.1"
                     /translation="MKAHAITDRIYAIHADVRTEDLFEGIWPIPDGVSLNSYLLKGDK
                     IALVDLVRDWGGAPAALETGLSQAGVKLADVDYLVLNHLEPDHTGWLAEYRRINPKVQ
                     ILATPKGVELLKSFYKVSEGVRAVKTGDELDLGAGLKLSFIEAPNVHWPETMLSYEPG
                     SGTLFSCDAFGSFGMLGDRVFDDQFNEGEHERYEEEGLRYYANIVASFGLFVKRAVEK
                     LKGLDIRTVAPSHGMIWRAKPRTIIDRYLRYAGYLDGKREKEVCVVWGSMYGNTEKGL
                     KAVLAGLEDEGVPYSMHRVPNEDASYILADAVKSEGILIAMPTYEYKMFPPMAYVLDL
                     FDRKHVWGRTALRIGSWGWVGGARKEYEEAIAPLKWNSLESVEWPGVPDEAAL"
     gene            1323..3590
                     /locus_tag="VFL04_07790"
     CDS             1323..3590
                     /locus_tag="VFL04_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7839645.1"
                     /translation="MNGSTAVRADSPGADISPWWRYGVLVVFLVGFGVLVWISAGAYK
                     YGPPIPDRVLGPDGAVVFTGEDVKAGQEVFLKYGLMDNGTVWGHGAYLGPDFSASYLH
                     NLSEDVAASLPPEGPAGVPAGTRVASVLSENRYESSGKVLRFTAPEVASYQRQIGLWT
                     EHFASSSSSRGLPPASISDPKEMRQLVAFFAWTAWASAAHVPGRDHSYTNNFPYDPQA
                     GNFPSRDSLIWSALSFIALLAGIGAVLFAFGRFDYLGWKGDTEHPVAFRMPTGVSPTQ
                     RSTLKFFVVVLLLMLAQTLLGGAVAHFRADPKSFYGLDLASILPSNLLRTWHLQTAVF
                     WIATAYIGGGLFIAALIGKKDPKGQAALSHILFAALFVVVAGSLLGELLGLRQLLGKL
                     WFWIGSQGWEYLEIGRAWQFALAVGLLLWVFLVYRGLGSAMRDPEQRELNRLFLAAAI
                     AIPLFYLPAFFYDGRTTYTVVDTWRFWIIHLWVEGFFELFATTMVAVMFYNLGMVTAK
                     TAKRVIYLDALLFLGAGMIGTGHHWYWIGQTTFSMAMSSVFSAMEVVPLILLTLDASA
                     FMRLTRGAQGTGGKDYLPHRWTFYFLISVGVWNFVGAGVFGFLINTPIVSYYEVGTTL
                     TPNHGHAAFMGVFGMLAMAFVVFVLRQVSDEAHWKRIERWVKTSFWGMNLGLGGMVVL
                     SLFPVGLLQLSDVLRNGYWHARSAAFMDRPVVSVLEYLRLPADLAFIILGVLPLLYAV
                     VSTYLRARKAPAEGA"
     gene            3639..4253
                     /locus_tag="VFL04_07795"
     CDS             3639..4253
                     /locus_tag="VFL04_07795"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7839646.1"
                     /translation="MDSDTGLRIVSAALYLSVFVIRIFYAARHRRLDYRKVDRRRSIA
                     AEGPLIYWGTSLAGAVLPLALAASFYDTGLELGFLPLGLRLGFLPLAILGILLFARSH
                     ADLGSRWSPDLELAPDHELVTGGVYARIRHPMYAGGALFYLGLALANADALVVACCLA
                     MVILQDARIGREEEMMAGRFGDAYQAYRARTGRYFPRLGRPRPL"
BASE COUNT          602 a         1530 c         1380 g          789 t
ORIGIN      
        1 ccagggcggc ctcgtcgggg acgccgggcc actcgaccga ctcgaggctg ttccacttga
       61 ggggggcgat ggcctcctcg tactccttcc tcgccccgcc gacccagccc catgagccga
      121 tgcggagggc cgtccggccc cagacgtgct tccggtcgaa gaggtcgagg acgtaggcca
      181 tgggcgggaa catcttgtac tcgtaggtgg gcatcgctat gaggatgccc tcgctcttca
      241 cagcgtcggc gaggatgtag gaggcgtcct cgttggggac gcggtgcatg gaatagggca
      301 cgccctcgtc ctcgaggccc gcgaggacgg ccttgaggcc cttctcggtg ttgccgtaca
      361 tcgagcccca gacgacgcag acctccttct cgcgcttgcc gtcgaggtag cccgcgtacc
      421 tgaggtagcg gtcgatgatg gtccggggct tcgcgcgcca gatcatgccg tgggaggggg
      481 ccacggtcct gatgtccagg cccttgagct tctcgacagc ccgcttcacg aagaggccga
      541 agctcgcgac gatgttcgcg tagtagcgca ggccctcctc ctcgtagcgc tcgtgctcgc
      601 cctcgttgaa ctggtcgtcg aagacccggt cgccgagcat gccgaagctc ccgaaggcgt
      661 cgcaggagaa gagggtgccc gagcccggct cgtagctgag catggtctcg ggccagtgca
      721 cgttgggggc ctcgatgaag ctgagcttga ggccggcgcc caggtcgagc tcgtccccgg
      781 tcttgaccgc cctgacgccc tcgctgacct tgtagaagct cttgaggagc tcgaccccct
      841 tgggggtcgc gaggatctgg accttcgggt tgatcctgcg gtactcggcc agccaacccg
      901 tgtggtcggg ctcgaggtgg ttgaggacga ggtagtcgac gtcggcgagc ttgaccccgg
      961 cctgggagag gccggtctcg agggcggcgg gggccccgcc ccagtcccgc acgaggtcga
     1021 cgagggctat cttgtcgccc ttgaggaggt atgagttgag agagaccccg tcggggatgg
     1081 gccagatgcc ctcgaagagg tcctcggtcc tgacatcggc gtggatggcg tagatcctgt
     1141 cggtgatggc gtgggctttc atggctccac tatacgcgag cgggggaggg ggaatcaacc
     1201 gagccctccc aaagcccgga tcatgggcgg cggcttgtcc ccgctcgcgg tgggcatata
     1261 atgttaacga cggccccggc cgcgtcgtcc tttccgtgcg tcgggaccaa aaggaggtcg
     1321 gcatgaacgg atcgacggct gtccgcgcgg attcccccgg agcggacatc tccccctggt
     1381 ggcgctacgg ggtcctcgtc gtgttcctcg tgggcttcgg cgtccttgtc tggatctcgg
     1441 cgggggccta caagtacggc ccgcccatac ccgatcgggt cctcgggccc gacggcgctg
     1501 tagtcttcac cggcgaggac gtaaaggcgg gccaggaggt cttcctcaaa tacgggctca
     1561 tggacaacgg cacggtctgg ggccatggcg cctatctcgg ccccgacttc tcggccagct
     1621 acctccacaa cctctccgag gacgtcgccg cctccctccc ccccgaaggc cccgccgggg
     1681 tccccgccgg gaccagggtc gcctcggtcc tctccgagaa ccgctacgag tcctcgggca
     1741 aggtcctccg cttcaccgct cccgaggtgg cgtcctacca gcgccagatc gggctctgga
     1801 ccgagcactt cgcgagctcg agcagctcga ggggcctgcc ccccgcctcg atctccgatc
     1861 ccaaggagat gcgccagctc gtggccttct tcgcctggac ggcctgggcc tcggccgccc
     1921 acgtgccagg ccgcgaccat tcctacacga acaacttccc ctacgacccc caggccggga
     1981 acttcccctc ccgggattcc ctcatctgga gcgccctcag cttcatagcc ctcctggcgg
     2041 gcatcggggc cgtgctcttc gccttcggcc gcttcgacta tctgggctgg aagggcgaca
     2101 ctgagcatcc cgtcgccttc cggatgccca ccggggtgag cccgacgcag aggtcgaccc
     2161 tcaagttctt cgtggtggtc ctcctcctca tgctggccca gaccctcctc ggcggggcgg
     2221 tggcccactt ccgcgccgac ccgaagagct tctacggcct cgacctggcc tcgatcctcc
     2281 cctccaacct gctgcggacc tggcacctcc agaccgccgt gttctggata gccacggcct
     2341 acatcggcgg agggctcttc atcgccgcac tgatcggcaa gaaggacccg aagggccagg
     2401 cggcgctgag ccacatcctc ttcgccgccc ttttcgtcgt ggtggctggg agcctcttgg
     2461 gcgagctcct cggcctccgc cagctccttg gcaagctctg gttctggatc gggtcccagg
     2521 gctgggagta cctcgagata ggcagggcct ggcagttcgc cctggccgtc ggactcctgc
     2581 tttgggtctt cctcgtgtac cggggcttgg gctcggccat gagggatccc gagcagaggg
     2641 agctcaaccg gctcttcctc gccgccgcga tcgcgatccc cctgttctac ctgccggcct
     2701 tcttctacga cggccgcacg acctacaccg tcgtcgacac ctggcgcttc tggatcatcc
     2761 acctctgggt cgagggcttc ttcgagctct tcgcgaccac gatggtcgcg gtgatgttct
     2821 acaacctcgg gatggtcacg gccaagaccg ccaagcgagt catctacctc gacgccctgc
     2881 tcttcctcgg cgcggggatg atcggcacgg gccaccactg gtactggata ggccagacca
     2941 ccttctccat ggccatgagc tcggtgttct cggccatgga ggtggtgccc ctcatcctcc
     3001 tcaccctcga cgcctcggcc ttcatgaggc tcacccgggg ggcccaaggc acggggggca
     3061 aggactacct cccccaccgc tggaccttct acttcctcat ctccgtggga gtctggaact
     3121 tcgtcggcgc gggcgtcttc ggcttcctca tcaacacgcc gatcgtgagc tactacgagg
     3181 tgggcaccac cctgacaccc aaccacggcc acgcggcctt catgggggtc ttcggcatgc
     3241 tcgcgatggc cttcgtggtc ttcgtgctga ggcaggtctc cgacgaggcg cactggaagc
     3301 ggatcgagag gtgggtgaag acctccttct gggggatgaa cctcggcctc ggcggcatgg
     3361 tcgtcctgag cctcttcccc gtgggcctgc tccagctcag cgacgtgctc cgcaacggct
     3421 actggcacgc gcgctcggcg gccttcatgg acaggcccgt ggtcagcgtc ctcgagtacc
     3481 tgcgtctgcc ggccgacctc gccttcatca tcctcggcgt cctgcccctg ctgtacgccg
     3541 tcgtctcgac ctatctcagg gcccgcaagg cccctgccga gggggcatag gagctcaagt
     3601 ctggaccgaa gggggccagc cgggacatac tcgcgctcat ggacagcgac accggcctca
     3661 ggatcgtctc ggcggccctc tacctctcgg tcttcgtcat caggatcttc tacgcagcgc
     3721 gccaccggcg gctcgactac aggaaggtcg acaggaggcg ctcgatcgcc gccgagggcc
     3781 ccctcatcta ctggggcacg agcctcgccg gcgccgtcct gccgcttgcc ctggccgcct
     3841 cgttctacga tacggggctc gagcttggct ttctgcccct cggcctccga ctcggcttcc
     3901 tccccctggc catcctcggg atcctcctct tcgcgcgctc ccacgccgac ctcggctcgc
     3961 gttggtcgcc cgatctcgag ctggcgcccg accacgagct cgtgaccggg ggggtctacg
     4021 cgaggatccg ccaccccatg tacgcaggcg gagccctctt ctacctcggc ctggccctgg
     4081 ccaacgccga cgccctcgtc gtggcctgct gtctcgcgat ggtcatcctc caggacgcga
     4141 ggataggccg ggaggaggag atgatggcgg gccgctttgg cgatgcctac caggcctacc
     4201 gggcgaggac gggccgctat ttcccgaggc tcgggcggcc gagacccctc taggcctcgg
     4261 ccgcgtccgc ggatcgcccg gccgggcccc gggcacgctc c
//
LOCUS       DASSQV010000445         5723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_600998, whole genome shotgun sequence.
ACCESSION   DASSQV010000445 DASSQV010000000
VERSION     DASSQV010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600998"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFL04_07800"
     CDS             <1..540
                     /locus_tag="VFL04_07800"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7839647.1"
                     /translation="AEMNERFLREKLAPGPLLTRIGINTGEMTVGNMGTARRMDYTMM
                     GNAVNLAARLEGVNKQYGTWILVSEGTRGRLDDSVVLRKLDRVRVVGIATPVRLFEVC
                     AFRSEIGDRGLEKIALFEQGIDAYEGRDWDAAAKRFEAVLALEPTDGPARTFLERTAK
                     SRAAGYGPDWDGVFSLTST"
     gene            complement(550..1548)
                     /locus_tag="VFL04_07805"
     CDS             complement(550..1548)
                     /locus_tag="VFL04_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7839648.1"
                     /translation="MSGEVLLVGLAGVLTAAAPIIFAVIGETITERAGVINLSMNGMI
                     LLTAMGGFAVSLATGSLALGFLAGAAIGAIVALIVAFASITIRASQVAVGFVLALLCR
                     DLSYFLGNPIMGEAGLRLPSQSIPLLKDIPVLGVLLFRQDLMTYLSFLLVFAAWYFIF
                     RTRPGLVLRGIGEKPAAAYVRGANVNRLRYVYTVLGGAIAGLAGPIYSLSVKAGWKGT
                     ISGLDGIGWIVLAITIFGGWNPFRAAFGAYLFAFLQWLGLVLQSALPGIPTQVLQVAP
                     FPLMILSLLLVNIGNAEWVEMSLARLPPPARRFFSRLLRAMNATPPAALGTPFEKD"
     gene            complement(1545..2600)
                     /locus_tag="VFL04_07810"
     CDS             complement(1545..2600)
                     /locus_tag="VFL04_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7839649.1"
                     /translation="MRDTRTNRALLQTGAVALALALTTVVLLAAKAPPLAAFANIALG
                     ALGSWEVVSNVLVSWVPLLLVTSGLLLTFTVGLWNIGIEGQITVGAIFSTWAFRLLLG
                     SGLAPGLVILAAILAGMLGGALWAMLAGALKTFGGVNEIFGGLGLNFVATALNLWLIF
                     GPWKRPGVASMSGTAPFDPSLWLPTLNPQSRLSPIAVGIALAAILVVFVMLRGTYFGL
                     RLKAVGKNPKAAYLLGIPTWQYMMASFALCGLFAGLAGAVQVSSVYHRLIPSISSGYG
                     YLGLMVAMLVNYRAALAAPVALFFAALNVGSIQLPIVLQLDSSLAGVLQGSLVLVVIL
                     AQGARSRLLKKEGGMKP"
     gene            complement(2593..4086)
                     /locus_tag="VFL04_07815"
     CDS             complement(2593..4086)
                     /locus_tag="VFL04_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7839650.1"
                     /translation="MRVELRDIRKIYGPVRANDGISLAVPAASIQGILGENGAGKSTL
                     MKILSGFILPDAGGILLDGEPVSIRSPADAIRLGIGMLHQDPLDFPPMKLIDDFILGR
                     PGGLGLDKRAAAKEFRALAAQCDFALEPEAWVDSLTVGERQQLEILRLLWLGARVLIL
                     DEPTTGISLPQKEKLFATLKRLAGEGMTVIFVSHKLEDVEFLCDRAAVLRQGRLIGEA
                     LPPFDTRRLVEMMFGKEVSLAERTTTARASGILQVKGVSLESLRIQMRNLSFEVRAGE
                     VIGLAGMEGSGQSTFLGACAGLTRPVEGSFCIEDEDMTGRSHHDYKREGVAYLPAARL
                     EEGLVPGLSLTEHFVLAEEMKGLFIDRRRAQRLTEERIALYNIKGRPESSVESLSGGN
                     QQRMLLALLRSPLSLILVEHPTRGLDIESTIYIWSKLKERCAEGTGIVFISADLDEVL
                     QYSDRVIVFFAGKVSPPLPAASLSVDRLGALIGGKGWEELEKEVAHA"
     gene            complement(4233..5438)
                     /locus_tag="VFL04_07820"
     CDS             complement(4233..5438)
                     /locus_tag="VFL04_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7839651.1"
                     /translation="MRNFARIVTLLLAVAVCGTAFAQGAAPAAAGNSLTIGLLMVGPY
                     NDHGYSQAHFDGAQYAVKNVKGANLIYLDKVNPADRPGTTASQLAESLVAKGAKLVIF
                     SSDDMSDEAVKFAKAHPDLFVIMASGDQVWQEGKIALKLPNMVNIMGRMEYGKMIAGV
                     AAALTTQTGKIGYLGPLINDETRRLASSVYLVAKYAWNNYLKKDPAKLEFKVTWIGFW
                     FNIPGVTSDPTQVADDFFNSGYDVVVSGIDTTEALTEATKLTGQGKKVYAIPYDYADA
                     IKEGAGAALGVPYFNWGPAYANVFKSAMTGSWKSHFEWNGPDWKNINNPDTSAVGFNK
                     GKALSKDASAGVDRFVAELAKGLNLWKGPLNLQDGSAYLKAGVAATDQQVWYLPQLLQ
                     GMQGQSVSK"
     gene            complement(5501..>5723)
                     /locus_tag="VFL04_07825"
     CDS             complement(5501..>5723)
                     /locus_tag="VFL04_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839652.1"
                     /translation="VARARARDGMGTRAILTRIWKQRGLWARRRAGGRPVVVLWNDGS
                     GPALRRAVERALTRARGAIELVALRDSSL"
BASE COUNT          942 a         1786 c         2136 g          859 t
ORIGIN      
        1 gccgagatga acgagcgctt cctgcgcgag aagctggccc ccggccccct cctcacgcgg
       61 atcggcatca acaccgggga gatgaccgtc ggcaacatgg gcacggctcg gcgcatggac
      121 tacacgatga tgggcaacgc cgtgaacctc gcggccaggc tcgagggggt caacaagcag
      181 tacggcacct ggatcctcgt cagcgagggc acgcgcgggc gcctcgacga ctccgtggtc
      241 ctccgcaagc tcgaccgcgt cagggtcgtg ggcatcgcca ccccggtgcg gctcttcgag
      301 gtctgcgcct tccgctcgga gatcggggac cgcggccttg agaagatcgc cctgttcgag
      361 caggggatcg acgcctacga ggggcgggac tgggacgcgg cggcgaagcg cttcgaggcc
      421 gtcctcgccc tcgagcccac cgacggaccg gccaggacct tcctcgagcg cactgccaag
      481 agccgggccg ccggctacgg ccccgactgg gacggggtgt tcagcctgac gagcacgtga
      541 gagacccccc tagtccttct cgaagggcgt ccccaaggcc gccggcggcg tcgcgttcat
      601 ggcgcggagg agccgcgaga agaagcgcct agcgggcggc ggcagcctgg ccagactcat
      661 ctccacccac tcggcgttgc cgatgttcac gaggaggagg ctcaggatca tgagggggaa
      721 gggcgcgacc tggaggacct gggtggggat cccgggcagg gcgctctgga ggacgaggcc
      781 gagccactgg aggaaggcga agagataggc gccgaaggcg gccctgaagg ggttccagcc
      841 gccgaagatc gtgatggcaa ggacgatcca gccgatgccg tcgaggcccg agatcgtgcc
      901 cttccagcct gccttcaccg agagcgagta gatggggccg gcgaggccgg ctatcgcgcc
      961 gccgaggacg gtgtagacat agcgcagccg gttcacgttg gccccgcgca cgtaggcggc
     1021 ggcgggcttc tccccgatgc cgcgcaggac caggcccggc ctcgtgcgga agatgaagta
     1081 ccaggcggcg aacacgagga ggaagctcag ataggtcatc aggtcctggc ggaacaaaag
     1141 gacgccgagg acggggatgt ccttgagcag ggggatggac tgggagggaa ggcggagccc
     1201 ggcctcgccc atgatggggt tgccgaggaa gtaggagagg tctcggcaca ggagggcgag
     1261 gacgaagccc acggcgacct gggaggccct gatggtgatg ctcgcgaagg ccacgatgag
     1321 ggccacgatg gcgcctatcg cggctcccgc gaggaagccg agggcgaggc tccccgtcgc
     1381 cagggagacg gcgaagcccc ccatcgcggt gaggaggatc atgccgttca tcgagaggtt
     1441 gatgacccca gcgcgctcag tgatggtctc gccgatgacg gcgaagatga tgggcgcggc
     1501 cgcggtgagg accccggcca ggccgacgag caggacctcg ccgctcatgg cttcatcccc
     1561 ccttccttct tgaggagacg cgaacgcgcc ccctgggcca ggatgacgac gaggaccagg
     1621 ctgccctgga ggacccccgc gaggctcgag tcgagctgga gcacgatggg cagctggatc
     1681 gagcccacgt tgagcgccgc gaagaagagg gccacggggg cggcgagggc cgcgcggtag
     1741 ttcacgagca tcgccaccat gaggcccaga tagccgtagc ccgaggagat cgaggggatc
     1801 aggcggtggt agaccgagga gacctggaca gcccccgcga ggccggcgaa gaggccgcag
     1861 agggcgaagg aggccatcat gtactgccag gtcgggatcc cgaggaggta ggccgccttg
     1921 gggttcttgc cgaccgcctt gagcctcagc ccgaagtagg tgcccctgag catcacgaag
     1981 acgacgagga tcgcggccag ggctatgccc acggcgatgg ggctcaagcg ggactggggg
     2041 ttcagggtgg gcagccagag cgaggggtcg aagggcgcgg tgcccgacat cgaggccacc
     2101 cccgggcgct tccatggccc gaagatcagc cagaggttca gggcggtggc cacgaagttg
     2161 aggcccagac cgccgaagat ctcgttcacc ccgccgaagg tcttgagggc ccccgcgagc
     2221 atggcccaga gggccccgcc cagcatgccg gcgaggatgg cggcgaggat gacgaggccg
     2281 ggggcgaggc ccgagccgag gaggaggcgg aaggcccagg tcgagaagat cgcgcccacg
     2341 gtgatctggc cctcgatgcc gatgttccag agccccacgg tgaaggtgag gaggaggccc
     2401 gaggtcacga ggaggagggg cacccaggac acgaggacgt tggacaccac ctcccaggac
     2461 ccgagggcgc cgagggcgat gttcgcgaag gcggcgaggg gaggggcctt ggcggccagg
     2521 aggacgaccg tggtcagggc gagggcgagg gcgacggccc ccgtctggag gagggcgcgg
     2581 ttggtcctcg tgtcacgcat gggccacctc cttctccagc tcctcccagc ccttgccgcc
     2641 gatgagggcg ccgagcctgt ccacgctgag cgaggccgcg ggcaggggag gcgagacctt
     2701 gccggcgaag aagacgatga cccggtccga gtactgcagt acctcgtcga ggtcggccga
     2761 gatgaacacg atgcccgtcc cttcggcgca gcgctccttg agcttgctcc agatgtagat
     2821 cgtcgactcg atgtcgaggc cccgggtcgg gtgctcgacg aggatgaggg agagggggga
     2881 gcgcaggagg gccaggagca tgcgctgctg gttgccgccc gagagggact cgacgctgct
     2941 ctcgggcctg cccttgatgt tgtagagggc gatgcgctcc tcggtgagcc tctgggccct
     3001 gcggcggtcg atgaagaggc ccttcatctc ctcggcgagg acgaagtgct cggtgaggga
     3061 gaggcccggc accaggccct cctcgagccg ggcggccggg aggtaggcca cgccctcgcg
     3121 cttgtagtcg tggtgggagc ggccggtcat gtcctcgtcc tcgatgcaga aggagccctc
     3181 gacggggcgg gtgaggccgg cgcaggcgcc gaggaaggtg gactggcccg agccctccat
     3241 gccggcgagg ccgatgacct cgcccgcgcg gacctcgaag ctcaggttgc gcatctggat
     3301 cctcaggctc tcgagcgaca ctcccttcac ctggaggatg cccgaggccc tcgccgtcgt
     3361 cgtgcgctcc gcgaggctca cctccttgcc gaacatcatc tcgacgaggc ggcgggtgtc
     3421 gaaggggggc agggcctcgc cgatgagcct gccctggcgc aggaccgccg cgcggtcgca
     3481 gaggaactcg acgtcctcga gcttgtgcga gacgaagatg acggtcatgc cctccccggc
     3541 cagacgcttc agggtggcga agagcttctc cttctggggc aggctgatgc cggtcgtggg
     3601 ctcgtccagg atgaggacgc gggcgccgag ccagaggagt cggaggatct cgagctgctg
     3661 gcgctcgccg acggtgagcg agtcgaccca ggcctcgggc tcgagggcaa agtcgcactg
     3721 tgcggctagg gcgcggaact ccttggcggc ggcccgcttg tcgagcccga gcccgccagg
     3781 gcggccgagg atgaagtcgt cgatcagctt catcgggggg aagtcgaggg ggtcctggtg
     3841 gagcatcccg atgcccaggc ggatggcgtc ggcgggggag cgtatggaca ccggctcgcc
     3901 gtcgaggagg atcccgcccg cgtcgggcag gatgaaaccc gagaggatct tcattagggt
     3961 ggacttgcca gccccgttct cgccgaggat gccctggatc gaggcggccg gcaccgcgag
     4021 ggaaatcccg tcgttggccc tcaccggacc gtagatctta cgaatgtcgc gcagctcgac
     4081 ccgcatcgaa tgacccctta ggaaaaatgc aaccacggag acggggagtc tgggagagag
     4141 cacggagaag agtctgatct ctcctccgtg atctccccct tcctccagcg ccctgcgcta
     4201 gctcgggcgc tgctccgtgg ttcctcgtgc tcttacttcg agacgctctg gccctgcatg
     4261 ccctggagga gctggggcag gtaccagacc tgctggtcgg tggcggccac tccggccttc
     4321 aggtaggcgc tgccgtcctg gaggttcagc gggcccttcc agaggttgag gcccttggcg
     4381 agctcggcga cgaacctgtc aacgccagcg ctcgcgtcct tggagagggc cttgcccttg
     4441 ttgaagccga ccgccgaggt gtcggggttg ttgatgttct tccagtccgg gccgttccac
     4501 tcgaagtggg acttccagga gccggtcatg gccgacttga acacgttcgc gtaggcgggg
     4561 ccccagttga agtagggcac gccgagggcg gcgccggcgc cctccttgat cgcgtccgcg
     4621 tagtcgtagg ggatggcgta gaccttcttg ccctggccgg tgagcttggt ggcctcggtg
     4681 agggcctcgg tggtgtcgat gccggagacc acgacgtcgt agcccgagtt gaagaagtcg
     4741 tcggcgacct gggtcgggtc ggaggtgacg ccggggatgt tgaaccagaa gccgatccag
     4801 gtgaccttga actcgagctt ggcagggtcc ttcttgaggt agttgttcca ggcgtacttg
     4861 gcgacgaggt agaccgagga ggcgaggcgg cgggtctcgt cgttgatgag gggcccgagg
     4921 tagccgatct tgccggtctg ggtggtgagg gcggcggcca cgccggcgat catcttgccg
     4981 tactccatgc ggcccatgat gttgaccatg ttggggagct tgagggcgat cttgccctcc
     5041 tgccacacct ggtcgcccga ggccatgatc acgaagagat cggggtgggc cttggcgaac
     5101 ttcaccgcct cgtcgctcat gtcgtcggag ctgaagatga cgagcttggc gcccttggcg
     5161 acgaggctct cggcgagctg ggaggccgtg gtgccggggc ggtctgccgg gttcaccttg
     5221 tcgaggtaga tcaggttggc gcccttgacg ttcttgacgg cgtactgggc cccgtcgaag
     5281 tgggcctggc tgtagccgtg gtcgttgtag gggccgacca tcagcaggcc gatcgtgagc
     5341 gagttgcccg cggcggcggg ggccgctccc tgggcgaagg ccgttccgca taccgcgacg
     5401 gcgaggagca gcgtgacgat cctggcgaag ttcctcattg tccctctcct tgtgccggtt
     5461 ccgccgcctt ctggcggcct gggctccggc tagaggcgga ttatagactc gagtcgcgga
     5521 gggcaacaag ctcaatcgcc ccccttgccc gggtcagggc ccgctccacc gcccggcgca
     5581 gggccggccc cgagccgtcg ttccagagga ccaccacggg tcttccgccc gccctcctcc
     5641 tggcccaaag cccgcgctgt ttccagatcc tggtcaggat ggccctggtg cccatgccgt
     5701 cgcgggccct cgccctcgcg acc
//
LOCUS       DASSQV010000446         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2343353, whole genome shotgun sequence.
ACCESSION   DASSQV010000446 DASSQV010000000
VERSION     DASSQV010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2343353"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VFL04_07830"
     CDS             <1..1029
                     /locus_tag="VFL04_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7839653.1"
                     /translation="VFAGFRIKEAIQIAKLQVLGKEAAAELARRALSFEIRRAYWESV
                     RATANEQVLERNLELEKLSRDDVRNLVEQGMATTADSLAEDARYDQTTLALDDSKSLL
                     ELALLQLSTLIGDRNATGSAAAEAGYELSSKPGEVPPPAPELAGPVKEAALVEAALAN
                     RPESRGAVAALNAANHALVASRADLYPSVLVTGNVSYADPDPRLFPPVDKFNLTWSAG
                     VRLRYELGGMPGAIERGKAAEADLEKARADIARSRNGIALDVRRCVLALNRSQTSLEL
                     TRGLVGQAEESLRVTRQKYDNGLAKNSDVLQAEIAVLRAGFAVESKAIDVEIARADLA
                     RAAALGTL"
     gene            1182..1832
                     /locus_tag="VFL04_07835"
     CDS             1182..1832
                     /locus_tag="VFL04_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7839654.1"
                     /translation="MLVREVMTMDPVTVASDVSVPDALALMREKKVRRLPVLDAKKRL
                     VGIVSDKDLLYASPSPSTSLNVWEIHSLLSKLTIDKVMTRDVVTIVEDTPVEQAARIL
                     AEKKIGGLPVMRGADLVGIITETDIFSAFTELLGERRHGVRIYATTSGEKGTVAKVAG
                     AIFAAGGDIVGLATAEAKLGGVVRWTITVKVQDVPRDKLVAAVKGTVLEILDVRES"
     gene            2120..>2778
                     /locus_tag="VFL04_07840"
     CDS             2120..>2778
                     /locus_tag="VFL04_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET7839655.1"
                     /translation="MNILFIVVGAGLVLLIGYLTYGRFLAKHVFQLDDSRKTPAVEME
                     DGVDYVPADAKLLAGQHFSAIAAAGPITGPILAGTLFGWVPALIWIIVGSIFIGGVHD
                     MGALIASVRHKARSITEVVKENVSRRAYVLFMLFIWITLVYIIVAFTDITAGSFVGVI
                     ALENGAKVAGGAIATSSLLYLALPIVMGLFLTFAKMKLWLATVIFLPLVGVAIWIGQY
                     IP"
BASE COUNT          454 a          993 c          891 g          440 t
ORIGIN      
        1 gtcttcgccg gcttccggat caaggaggcc atccagatag cgaagctcca ggtgctcggg
       61 aaggaggccg ccgccgagct ggcgaggcgg gccctctcct tcgagatccg ccgcgcttac
      121 tgggagtcgg tgcgcgccac ggcgaacgag caggtgctcg agcgcaacct cgagctcgag
      181 aagctcagcc gcgacgacgt gcgcaacctg gtcgagcagg gcatggccac gacggccgac
      241 agcctggccg aggacgcgcg ctacgaccag accaccctcg ccctcgacga ctcgaagtcc
      301 ctcttggagc tcgccttgct ccagctctcc acgctgatcg gggaccggaa cgccaccggc
      361 tcggccgccg ctgaggcggg ctacgagctg tcctcgaagc caggcgaggt tccgccccca
      421 gcccccgagc tcgccggccc ggtcaaggag gccgccctgg tcgaggcggc cctggccaac
      481 aggcccgagt ccaggggcgc cgtcgctgcc ctgaacgcgg cgaaccacgc cctggtggcc
      541 tcgcgcgccg acctctaccc gagcgtcctc gtcaccggca acgtcagcta cgccgacccc
      601 gacccgaggc tgttcccccc ggtcgacaag ttcaacctca cctggtcggc gggcgtcagg
      661 ctccgctacg agctcggcgg catgcccggt gccatcgagc gcggcaaggc ggccgaggcc
      721 gacctcgaga aggcgagggc cgacatagcc cgctcgcgca acggcatcgc cctcgacgtc
      781 aggcgctgcg tcctcgccct gaaccgctcc cagacctcgc ttgagctgac ccggggcctc
      841 gtgggccagg ccgaggagag cctccgcgtt acgcggcaga aatacgacaa cgggctggcc
      901 aagaactccg acgtcctcca ggccgagatc gccgtcctgc gcgccggttt cgccgtcgag
      961 agcaaggcca tcgacgtcga gatagcccgc gccgacctcg cgcgggcggc ggccctcggc
     1021 acgctgtagg cgggcatgcg ggagtccgta ccggaccccg ccggatgacc gggaggggcg
     1081 cccacgggcg cccctcgctc ttttcctggg ccccggagct ggcggcccgg cggttcccgg
     1141 cattatattg agagggcagc cagagtacca aggaggcagt catgctggtc agagaggtta
     1201 tgacgatgga tcccgtcacg gtcgcgtccg acgtgtccgt gcccgacgcc ctcgcgctca
     1261 tgcgcgagaa gaaggttcgc cgcctgccgg ttctcgacgc gaagaagcgc ctcgtgggca
     1321 tcgtgtcgga caaggacctc ctctacgcct cgccctcgcc ctcgacgtcg ctcaacgtct
     1381 gggagatcca ctccctcctc tcaaagctca ccatcgacaa ggtgatgacc agggacgtgg
     1441 tgaccatcgt cgaggacacg ccggtcgagc aggccgcgag gatcctcgcc gagaagaaga
     1501 tcggcggcct gccggtcatg cgcggtgccg acctggtggg catcatcacc gagaccgaca
     1561 tcttctcggc cttcaccgag ctgttgggcg agcggcgcca cggcgtgcgc atctacgcca
     1621 ccacctcggg cgagaagggc accgtggcca aggtcgcggg cgcgatcttc gcggccgggg
     1681 gcgacatcgt gggcctggcc accgccgagg cgaagctggg cggggtcgtg cgctggacca
     1741 tcacggtcaa ggtccaggac gtgccgcggg acaagctcgt cgccgcggtc aagggcacgg
     1801 tcctcgagat cctcgatgtg agggaatcct aggcaagagg ccgccgactc gtcggggcgg
     1861 accgcatcat ccgcgccaag gagcgcgccc ttcccccggg gagggcgcgc ttttctttcg
     1921 ataaccaaag tttgtttcgc ttgcgcgatg ccccgcatgg gcataggata ggcgctaata
     1981 agcgcctgat tcaaaggagc cgggcgcgcc gcactcatcg atcaggtggg cggtgtagat
     2041 ttcataaagg ccggaacctg ttcgcggcgc atcggactcc cgaggaaggc gcaatttaac
     2101 acttcttcag ggggcacgaa tgaacattct cttcatcgtg gtcggagcgg gcctcgtact
     2161 cctcatcggg tacctgacct acggaaggtt cctcgccaag cacgtcttcc agctggacga
     2221 ctccaggaag acgcccgcgg tcgaaatgga ggacggggtc gactacgtcc ccgccgatgc
     2281 gaagctcctc gcgggccagc acttctcggc catcgccgct gccggcccaa tcaccggccc
     2341 gatcctcgcc ggcaccctgt tcggctgggt ccccgccctc atctggatca tcgtcggctc
     2401 gatcttcatc ggcggcgtcc atgacatggg cgccctcatc gcgtcggtga ggcacaaggc
     2461 gaggtccatc accgaggtcg tcaaggagaa cgtctccagg cgggcctacg tcctcttcat
     2521 gctcttcatc tggatcaccc tcgtctacat catcgtcgcc ttcaccgaca tcaccgccgg
     2581 ctccttcgtc ggcgtcatcg ccctcgagaa cggggccaag gtcgccggcg gcgccatcgc
     2641 cacctcgtcc ctgctctacc tggccctgcc catcgtcatg ggcctcttcc tcacgttcgc
     2701 gaagatgaag ctctggctcg ccaccgtgat cttcctcccc ctcgtgggcg tcgcgatctg
     2761 gatcggccag tacatccc
//
LOCUS       DASSQV010000447         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1719576, whole genome shotgun sequence.
ACCESSION   DASSQV010000447 DASSQV010000000
VERSION     DASSQV010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719576"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VFL04_07845"
     CDS             complement(<1..640)
                     /locus_tag="VFL04_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7839656.1"
                     /translation="MRVHFLGTGTSFGVPVIGCSCGTCRSRDPRDARYRSSALVTGAG
                     AAVLIDAGPEFRLQARRAGLGLRPGIERLDALLLTHAHADHVAGLDDVRPLTSERVLP
                     VYGNTAAIAETRDRFDYVFRETQIGGGKPRIELREAPELPFQVGGLGIRAIPLLHGAL
                     GILGWRFGRFAYITDCSSIPEPSLGLLEGVEVLAINGLRWRPHATHFTVDQGI"
     gene            complement(718..>2491)
                     /locus_tag="VFL04_07850"
     CDS             complement(718..>2491)
                     /locus_tag="VFL04_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7839657.1"
                     /translation="DGSGRLRGLFDSEVPLEGLNARLKAAVAGGTTQAFILTAAGEVI
                     ATSTGDSVTREGEGGKLQLLPAAESANPVFAKASAIDGADAVSAASEGNSTWVAELKA
                     EGKTYFISSSPFSDERGLDWRIVVVESVSTAMDILYANLRVGLVVGVLALILGLAVII
                     IVTRSISRSVGDIRTSLASLAAGDLRQQATRTAATEIGAIQKAVGELSAGLSTIILDV
                     RQAAEKSASTGEILAAHSAESAATITEISANIVSMRGQTQRLDGAAESAEKAKDGILS
                     ASQTVAGAVSDLEAATRTAVKLIEGIADGLRALSGKAQAQHDLASKVSALSAEGRNSV
                     EGAVASMRTMEESAAKTLELVGIINGIAEQTGLLAMNAAIEAAHAGDAGRGFSVVAEE
                     IRKLSESTAENAQGIGQTIEETSIAVREAGATTEQTRQSIGAAVDAVDRLIVELASVA
                     DSLGDLGRRSGEVLDAIETLTKTAGGLSGATSSLGEGAGVIARTVEDVRKLSSENRNA
                     AEEVAMGIHGIDESANMLSELSRENADTAAAIRVAVERFRLDDSVLPPPPELPAAPDE
                     DAQEETLAGPGPQERGIAIKRASV"
BASE COUNT          317 a          865 c          900 g          409 t
ORIGIN      
        1 cgatgccctg gtcgacggtg aagtgggtcg cgtggggcct ccaccgcagc ccgttgatcg
       61 cgaggacctc gaccccctcg agcaggccga ggctgggctc ggggatgctc gagcagtcgg
      121 tgatgtaggc gaaacggccg aagcgccagc ccaggatccc gagagcgccg tggaggaggg
      181 ggatggcccg gatgccgagg ccgccgactt ggaatgggag ctcgggcgcc tccctgagct
      241 cgatccgcgg cttccctccc ccgatctggg tctccctgaa cacgtagtcg aagcggtccc
      301 gcgtctccgc gatcgccgcc gtgttgccgt agacgggcag gacccgctcg ctcgtgagcg
      361 gcctcacgtc gtcgagcccc gccacgtggt cggcgtgggc gtgggtgagg aggagggcat
      421 cgagcctttc gatgccgggc ctgaggccga gcccggcccg tctggcctgg agtcggaact
      481 cggggccggc gtcgatgagg acagcggcac ccgcgcccgt cacaagggcc gagctgcggt
      541 atcgcgcatc gcgtggatcc ctcgatctgc aggttccgca ggagcagccg atgacgggca
      601 cgccgaagct ggtgcccgtg ccgaggaagt ggactcgcat ggcaaaggag tatggaccct
      661 tgggccccca cccgccaagg cgggtctccg ccggggtgga ggcccgcagg gcctggccta
      721 gaccgaggcg cgcttgatcg ctatcccccg ctcctggggg ccgggcccgg cgagggtctc
      781 ttcctgggcg tcctcatcag gggcggccgg aagctcgggg ggcgggggca ggaccgagtc
      841 gtcgaggcgg aagcgctcga cggccacgcg tatggccgcg gcggtgtcgg cgttctcgcg
      901 cgacagctcg gagagcatgt tggccgattc gtcgatcccg tggatgccca tggcgacctc
      961 ctcggccgcg ttccggttct ccgagctgag cttgcgcacg tcctcgaccg tcctcgcgat
     1021 gacgccggcc ccctcgccga gggagctggt ggcgcctgaa agcccgcccg ccgtcttcgt
     1081 gagggtctcg atggcgtcga ggacctcgcc gctcctccgg cctaggtcgc cgagggagtc
     1141 ggccacgctg gcgagctcga cgatgaggcg atcgacggcg tccaccgcgg ctccgatcga
     1201 ctgccttgtc tgctcggtcg tggccccggc ctcgcgcacg gcgatcgagg tctcctcgat
     1261 ggtctggccg atgccctggg cgttctcggc cgtgctctcc gagagcttcc tgatctcctc
     1321 ggcgacgacg gagaagcccc ttcccgcgtc cccggcgtgg gcggcctcga tcgccgcgtt
     1381 catggcgagg agtccggtct gctcggcgat cccgttgatg atcccgacga gctcgagggt
     1441 cttggcggcg ctctcctcca tcgtgcgcat cgaggcgacg gccccctcca cgctgttcct
     1501 gccctcggcg ctcagggccg agaccttcga ggccagatcg tgctgggcct gggccttccc
     1561 cgacagggcc cgcagtccgt cggctatccc ctcgatgagc ttgaccgctg tcctcgtcgc
     1621 cgcctcgaga tcgctcacgg cgccggcgac cgtctggctc gccgagagta tgccgtcctt
     1681 ggccttctcc gccgattcgg ccgccccgtc gagcctctgg gtctggcccc gcatggagac
     1741 gatgttggcg ctgatctcgg tgatggtggc cgccgactcg gccgagtggg cggcgaggat
     1801 ctcgcccgtg gaggcgctct tctcggccgc ctggcgcacg tcgaggatga tggtggagag
     1861 cccggccgag agctcgccga cggccttctg gatggcgccg atctcggtgg cggccgtgcg
     1921 ggtcgcctgt tgcctgaggt cgccggccgc gagcgaggcc aggctcgtcc tgatgtcgcc
     1981 caccgacctc gatatgctcc gggtcacgat gatgatgacg gcgaggccaa ggatgagggc
     2041 caggacgccg acgacgaggc caacgcggag gttggcgtag aggatgtcca tggccgtgct
     2101 gaccgactcg accacgacta tcctccagtc gaggccgcgc tcgtcggaga aggggctcga
     2161 gctgatgaaa taggtcttgc cctcggcctt gagctcggcg acccaggtgg agttgccctc
     2221 gctcgccgcg ctcaccgcat cggccccatc gatggccgag gccttcgcga acacggggtt
     2281 ggcgctttcg gcggccggga ggagctggag cttgccgccc tcgccctccc tggtcaccga
     2341 gtcccccgtg cttgtggcga tgacctcgcc cgcggcggtg aggatgaagg cctgggtcgt
     2401 ccctccagcc accgccgcct tcagccgcgc gttcaggccc tcgagcggga cctcggagtc
     2461 gaaaaggccg cggagccggc ccgagccgtc c
//
LOCUS       DASSQV010000448         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1097566, whole genome shotgun sequence.
ACCESSION   DASSQV010000448 DASSQV010000000
VERSION     DASSQV010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097566"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VFL04_07855"
     CDS             complement(<1..986)
                     /locus_tag="VFL04_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HET7839658.1"
                     /translation="MTRSTASRRLPELLLPAGSFESGIAAIEGGADALYLGFSSFSAR
                     KQARNLDPLEYRRLSGFARARGVRLYVAINPVILERELAAVSELLAFLSRFRPDSIIV
                     QDWGLARLIRQRHPGLTLHASTQTAVQGAEGARIARELGVTRIVLPRETPVAELARLR
                     AEVPDLEYEVFVHGALCYSYSGLCLASGLLLGRSGNRGECAQICRSYYSGEGPIGRKG
                     YWFSCRDLRLLERLESLAAAGAASLKVEGRMKAPEYCHAVARLYRGALDRLGGASLSD
                     TELEERAEAARLAFARSPTEAWLEERGGSCLIDSEYPGHRGVPVGVVVASTKG"
     gene            1194..2264
                     /locus_tag="VFL04_07860"
     CDS             1194..2264
                     /locus_tag="VFL04_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7839659.1"
                     /translation="MEETRITVKEIARLAGVSIGTVDRVLHDRGGVSLSTKRRIDEII
                     GSLGYEPNMLARQLSLNRNYEFRALLPHSGQDSGYWALCLEGVQRAAKELSPYRVRVR
                     VDQFDRYDRQAYGRLLEDVVRQPCDGLLIAPVLPRELAPALERLKGLIPYVFFDGSVE
                     GAAPIATIGQDAFRAGCLAGRVMSLLAGGSYPLVALNAHAEDSHIRLRIKGFASFFGG
                     QGAGPNDVFMRDFFETEDTEAYGAYLDALFDALPGIRGILVANASGHIVGDWLVRKGL
                     KTDCRLVAWDLVPANVHALSTGAIDCLVSQRPADQAKEGLERLFRSVVHRAEVGGVLK
                     MPLDLFFKENLPEPAQEPAAGT"
     gene            2342..3292
                     /locus_tag="VFL04_07865"
     CDS             2342..3292
                     /locus_tag="VFL04_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HET7839660.1"
                     /translation="MKRALLLLVGAAFLVALFGACAPAKPKVKTFVMVPKGVHPYYEP
                     CYQGFQAAAAKYGVKVDKVDPQKFELPLQVKVIEDLIAQKVDGIAISALDDAGLVPVI
                     HDAMKAGIKVITFDAPAPSSEALTYIGTDNKTAGSEAAKKMAELMKGEGDVIILQGGL
                     GATNLNLRTQGFKETMAQVAPKVKILDVVDEGGDFAQTVNKTEAILQTYPKLKAIFGV
                     SAEAAPGAAQVVKQQKKAGKIIIAGFDDLKDTLEGIKDGTVSFCLVQKTFKMGWLSVE
                     KLLDATAGKPIDKVIDTGVLIVDKSNVDNYMVDMKKEFAQ"
BASE COUNT          625 a         1122 c         1127 g          520 t
ORIGIN      
        1 cccttcgttg aggcgacaac gaccccaacg ggcacaccgc gatgccctgg atactccgaa
       61 tcgatgaggc acgagccccc cctctcctcg agccaggcct cggtcgggga ccgggcgaaa
      121 gccagccggg cagcctcggc cctttcctcc agctcggtgt ccgataggga ggctcccccg
      181 agccggtcga gggcgccccg atagagccgg gcaacggcgt ggcaatactc gggcgccttc
      241 atccgcccct ccaccttcag gctcgccgcg cccgctgcgg cgaggctctc gagccgctcg
      301 aggaggcgga ggtcgcggca cgagaaccaa tagcccttcc ttccgatcgg gccctcgccg
      361 gaatagtagg agcggcagat ctgggcgcat tccccgcggt tgcccgagcg gccgagcagg
      421 aggccggagg cgagacagag cccggagtag gaatagcaga gggcgccgtg gacgaacacc
      481 tcatactcca ggtcggggac ctcggccctg agcctggcaa gctcggcgac cggggtctcg
      541 cgaggaagga cgatgcgcgt gacgccgagc tcgcgggcga tcctcgcccc ctcggcgccc
      601 tggacggccg tctgggtcga ggcgtggagg gtgaggccag ggtggcgctg gcggatgagg
      661 cgggccaggc cccagtcttg gactattatg gagtcgggcc tgaaccggga gaggaaggcg
      721 aggagctcgc tcacggcggc gagctcgcgc tcgaggatga cagggttgat cgcgacatag
      781 agacggacgc cccgcgcgcg ggcgaatccg gacagacggc gatactcaag cgggtcgagg
      841 ttgcgcgcct gtttcctcgc cgaaaagctc gagaagccga ggtagagggc gtcggcccct
      901 ccttcgatcg cggcgatacc ggattcgaag ctgcctgcgg gcaacagaag ttcggggagc
      961 ctacggcttg ctgtgctgcg tgtcacgcca tgataatcgc cattcctcgc cctttggaca
     1021 agacatcgcg gaatgcagca gcacccgctc gcgccccgca ggcagggctg cggcgggagg
     1081 cgcgcgcgtt tacccgttga cggttcgggc gagtggtgct agggttccct tgtcgtgtac
     1141 gtacacgaga cgcgaatcct cacgacgcgc ggatcttgga cacgattgag cccatggaag
     1201 aaacgcggat cacagtaaag gagatcgccc gcctcgccgg ggtctccatc gggacggtcg
     1261 atcgggtcct ccacgaccgg ggcggagtct cgctctccac caagaggcgg atagacgaga
     1321 tcatcggatc cctcggctat gagcccaaca tgctcgcgcg tcaactctcg ctcaaccgga
     1381 actacgagtt cagggccctc ctgccccact ccggacagga ttccggctac tgggccctct
     1441 gcctcgaggg ggtgcagagg gcggccaagg agctgtcgcc ctaccgtgtg agggtcaggg
     1501 tcgaccagtt cgaccgttac gaccgacagg cctacggccg cctcctcgaa gatgtcgtgc
     1561 gccaaccctg cgacggcctc ctcatcgccc ccgtgctgcc cagggagctc gcccccgccc
     1621 tcgagcggct gaagggcctc ataccgtacg tcttcttcga cggaagcgtc gagggcgcgg
     1681 ctcccatcgc gaccatcggc caggatgcct tcagggccgg ttgcctggcg ggaagggtca
     1741 tgtccctcct cgccggagga agctaccccc tcgtggccct gaacgcccac gccgaggaca
     1801 gccacatcag gctgaggatc aagggtttcg cctccttctt cgggggccag ggggcgggtc
     1861 cgaacgacgt tttcatgcgg gacttcttcg agaccgagga caccgaggca tatggggcct
     1921 accttgacgc cctgttcgac gccctgccgg ggatcagggg catcctggtc gccaacgcct
     1981 ccggccacat cgtaggcgat tggctcgtga ggaaggggct caagacagac tgccgtctcg
     2041 ttgcgtggga cctcgtgcca gccaacgtgc atgccctctc caccggagcc atagactgcc
     2101 tcgtgtcgca gcgccccgcc gaccaggcga aggagggcct cgagaggctc ttcaggtcgg
     2161 tcgtccaccg cgccgaggtc ggcggggtcc tcaagatgcc cctcgacctg tttttcaagg
     2221 agaacctgcc cgagccggcg caggagccgg cagccgggac ctgatagccc atggaaggga
     2281 attgggggag gatggccttg agccgctctc ccttcaaaat ctttccctca aggagtgtct
     2341 gatgaagaga gcactgcttc tgctggtagg cgccgccttc ctggtggcgc tcttcggggc
     2401 atgcgccccc gccaagccca aggtgaagac cttcgtcatg gtccccaagg gcgtccaccc
     2461 ctattacgag ccctgctacc agggcttcca ggccgcggcc gccaagtacg gcgtgaaggt
     2521 cgacaaggtg gacccgcaga aattcgagct tccgctccag gtcaaggtca tcgaggacct
     2581 gatcgcccag aaggtggacg gcatcgcgat ctcggccctg gacgacgcgg gcctcgtgcc
     2641 cgtgatccac gacgcgatga aggccggcat caaggtgatc accttcgatg cccccgcccc
     2701 atcctcggag gccctcacct acatcgggac tgacaacaag acggcgggat ccgaggcggc
     2761 gaagaagatg gccgagctca tgaagggcga aggcgacgtg atcatcctcc agggcggtct
     2821 cggggccacg aacctcaacc tgcgcaccca gggcttcaag gagacgatgg cccaggtcgc
     2881 gcccaaggtg aagatcctcg acgtggtgga cgagggcggg gatttcgccc agaccgtgaa
     2941 caagaccgag gccatcctcc agacctaccc caagctcaag gccatcttcg gcgtctccgc
     3001 cgaggccgcg cccggggcgg cccaggtcgt caaacagcag aagaaggcgg ggaagatcat
     3061 catcgccggc ttcgacgacc tcaaggacac cctcgagggc ataaaggacg ggaccgtgtc
     3121 cttctgcctc gtccagaaga ccttcaagat gggctggctc tcggtcgaga agctgctcga
     3181 cgcgaccgcc ggcaagccga tcgacaaggt catcgacacg ggcgtcctca tcgtggacaa
     3241 gagcaacgtc gacaactaca tggtcgacat gaagaaagag ttcgcccagt gatggagcgg
     3301 catggctgac acgcgggccg gcctcggccg gcccgcgcat cctcccttgg gagcccgcaa
     3361 tggacatgag acgaaggccg gcacaggtca tgcg
//
LOCUS       DASSQV010000449         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2219997, whole genome shotgun sequence.
ACCESSION   DASSQV010000449 DASSQV010000000
VERSION     DASSQV010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219997"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VFL04_07870"
     CDS             complement(<1..286)
                     /locus_tag="VFL04_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7839661.1"
                     /translation="MEDHSHRLIEEHLRPFGLSLSQGFLFGLIVDWALKGIAPLQKDL
                     EKDMRLVTSSITNLVQGLERKGLISRTDSATDGRAKELHVTEKGWKVRGKL"
     gene            complement(430..2025)
                     /locus_tag="VFL04_07875"
     CDS             complement(430..2025)
                     /locus_tag="VFL04_07875"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7839662.1"
                     /translation="MDRPSSDLVLILISAALAAIAVAMAAFRRKRPLYIYAAGYACAA
                     LGFALLLGQGRLWPLVSVSLANTLNIGFQFCLAWGLRAWMEQPRPWPRRFHVYLGLCV
                     LAQVVFIGAAVPRAAAGALFAMLLSGEFLYALESGRRKVAPFMRMASRVIALAYMAGF
                     LLRLAMVLGLDHGGSGPGEQGLGGYEVGLLAFFSVLWGGLVLGIDIAGLLDELERKNR
                     SLSDMATTDNLTGLANRHQLDATVLGEIERASRYHEPLSLVLLDLDYFKRVNDTWGHA
                     TGDEVLVRAARLSQEMIRDTDRIFRWGGEEFLILTPHTNLEGAVVLAEKLRAALASEK
                     HPKVGRLTASLGVTEWGEGEGQAELLRRVDRALYRAKTLGRNRVASLAPGEAPPLSIV
                     RLDFHEEWESGDTRIDGEHRELLALVQEFLDRSVTALSSAEIVPYFERFFEHLVKHFA
                     DEEATLTALGYPGAAEHAAIHKGLIRSAEDQRSRLERGELGRAEFSRYLVDTIVLGHL
                     QVEDIKFFPWTKEADAGPPPSGR"
     gene            complement(2087..>2459)
                     /locus_tag="VFL04_07880"
     CDS             complement(2087..>2459)
                     /locus_tag="VFL04_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HET7839663.1"
                     /translation="GNRHSLVRRGGAGYFVEVRDGALVSRHRPSVDLLFRSAALCAGA
                     NAVGVIMTGMGDDGAHGMLELKEAGATTIAQDEDSCVVFGMPKEAIALGAVDYVLPLG
                     KIAAMAYTKALGRRGEWAGPG"
BASE COUNT          411 a          822 c          850 g          376 t
ORIGIN      
        1 caagcttccc gcgcactttc caccccttct ccgtgacatg gagctccttc gcccgcccgt
       61 ccgtcgccga atccgtgcgg gagatcaggc ccttgcgctc gagcccctgg acgagattgg
      121 tgatggacga ggtgaccagg cgcatgtcct tctcgagatc cttctggagc ggggcgatgc
      181 ccttcagggc ccaatcgacg atgagtccga acaggaatcc ctgggacagg gagagcccga
      241 agggccgcaa gtgctcttcg atgagcctgt gggagtggtc ctccacaagc cggaaccagt
      301 caagaaactc ggaactgagg aactctctgg attcgcccga catttggttc gacctcgaac
      361 taaatctaca gccagaccgg gaatctgtca aggctccagg atctggcctt cgagggccgg
      421 gtggaggcct cagcgcccgg agggcggcgg ccccgcgtcg gcctctttgg tccaggggaa
      481 gaacttgata tcctcgacct ggaggtggcc gaggacgatg gtgtcgacca ggtaccgcga
      541 gaactcggcg cggcccagct cccccctctc gagccgggac cgctggtcct cggcgctcct
      601 gatgaggccc ttgtggatcg ccgcgtgctc ggcggccccg gggtagccga gggcggtgag
      661 ggtggcctcc tcgtcggcga aatgcttgac caggtgttcg aagaagcgct cgaaataggg
      721 gacgatctcg gccgaactca gggcggtcac cgagcggtcg aggaattcct ggacgagggc
      781 gaggagttct cggtgctccc cgtcgatcct ggtgtcgccg gactcccatt cctcgtggaa
      841 gtcgagccgc acgatggata ggggcggggc ctcccccggg gccagactgg ccacgcggtt
      901 tcggcccaag gtcttggcgc ggtacagggc gcggtcgacg cgccggagga gctcggcctg
      961 tccctcgccc tccccccact ccgtcacccc gaggctcgcg gtgagcctgc ccaccttcgg
     1021 gtgcttctct gaggcaagcg cggcccgcag tttctccgcg aggacgacgg ccccctcgag
     1081 gttggtgtgg ggcgtcagga tgaggaactc ctcgcctccc cagcggaaga tgcggtcggt
     1141 gtccctgatc atctcctgcg agagccgcgc ggcgcggacg aggacctcat cgcccgtggc
     1201 gtggccccag gtgtcgttca cgcgcttgaa gtagtcgagg tcgaggagca cgagggacag
     1261 gggctcgtgg tagcggctgg ccctctcgat ctcgcccagt accgtggcgt cgagctggtg
     1321 gcggttggcg aggccggtga ggttgtcggt cgtcgccatg tcgctgaggc tgcggttctt
     1381 ccgctcgagc tcgtcgagga ggccggcgat gtcgatcccg agcaccaggc ccccccagag
     1441 caccgagaag aaggcgagga ggcccacctc atagccgccg aggccctgct cgccggggcc
     1501 cgagccgcca tggtccagcc cgaggaccat ggcgagcctg aggaggaagc ccgccatgta
     1561 ggcgagggcg atcacccggg aggccatcct catgaagggc gcgaccttgc gcctgccgga
     1621 ctcgagggca tagaggaatt ccccggaaag aagcatcgcg aagagcgcgc ccgccgccgc
     1681 cctcgggacg gccgcgccga tgaagacgac ctgggccagg acgcagaggc cgaggtagac
     1741 atggaagcgt cggggccagg gcctgggctg ctccatccac gcgcgcaggc cccatgccag
     1801 gcagaactgg aaaccgatgt tcagggtatt ggcgagggag accgagacca ggggccagag
     1861 cctcccttgg cccaggagca gggcgaaacc cagggcggca caggcatagc ccgccgcgta
     1921 gatgtacaag ggacgctttc tccggaacgc cgccatcgcg acggcgatgg ccgcgagggc
     1981 ggctgagatg aggatgagca ccaggtcgga cgagggtctg tccatcgcga tgagaatata
     2041 atgggccccg cccgagagac cagtgggcgc gccacgcccc cggcccctag cccggcccgg
     2101 cccattcccc tcggcgcccg agagccttcg tgtaggccat cgcggcgatc ttcccgagag
     2161 gcaggacata gtccacggcc ccgagcgcaa tggcctcctt cggcatgccg aacaccacgc
     2221 agctatcctc gtcctgggcg atggtggtgg ccccggcctc cttcagctcg agcatgccat
     2281 gggccccgtc gtcccccatc cccgtcatga tcaccccgac ggcgttcgct cccgcgcaca
     2341 gggcggccga gcggaagagg aggtccaccg aggggcggtg gcggctcact agtgctccat
     2401 ccctgacctc gacgaaatag ccggcgccgc cgcgcctcac caggctgtgg cgattgcct
//
LOCUS       DASSQV010000450         4942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2344860, whole genome shotgun sequence.
ACCESSION   DASSQV010000450 DASSQV010000000
VERSION     DASSQV010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2344860"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="VFL04_07885"
     CDS             <1..1234
                     /locus_tag="VFL04_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7839664.1"
                     /translation="EVQGSMMFDRLKAELDFPFVRCGIVVAAMHEDEMRGIGALYSQG
                     LRNGAVGIEMCSRERLLELEPKLSPDCVGGLYAPAGGIIEPYRFVFSLVESACKNGVR
                     LLTGWRLVAATRTGNRWLLRAADGRELAARFVVNAAGLFADEVSRALGAEDFRIQGRK
                     GEYYLLDRLTKARPDRVVFPVPSPISKGMLVIPTVEGTVLIGPTATKVDDKEDFATTR
                     EELEAILRSARHLIPAISEGDVITSFAGIRSVLGEDFYIELSAKAPALVQVAGIQSPG
                     LTASPAIGEYVKDLLKKPGLRLVEKADWDPYVEKVPRAKDMDPFELDAQARADPAFGN
                     IVCRCERVTEAEIVAAIRRGHTTLDGIKYFTRAQMGRCQGGFCTLRILKILMRETGMS
                     PEAITKRGGQSRVLGGEL"
     gene            1231..2487
                     /locus_tag="VFL04_07890"
     CDS             1231..2487
                     /locus_tag="VFL04_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7839665.1"
                     /translation="MKERSFDAVVVGGGAAGMAAALALDARGKRVAIVDREAGLGGIL
                     VQCIHNGFGLIEFNEELSGPEFAERLEEVVAKTRIAVFRGSTVFELEASGDEKTVVCV
                     SAALGVLRLHCRAVVLAMGCRERNRGNIRIPGTRPAGVFTAGLAQRLVNVEGYLPGKD
                     VVIIGSGDIGLIMARRMSWVGCRVHAVVEILPYPSGLTRNIVQCLHDVGIPLYLSHLT
                     TCIRGHDRVEGVQVTPFVNGALVPEKAFDIPCDTVLLSVGLVPENELTKKAGIDMNPA
                     TNGPAVDSTMMTSLAGVFACGNVLHVHDLVDYVVEESRRAGSFAADWLDGTRPGKEAR
                     IKVGPNVRYVSPGRIDLARENKVYLRSLVVKNDATLEVRLGNRLIRSIRKNHVQPSEM
                     ISLSLGGKELEPEASTAESVIEIAIR"
     gene            2498..2884
                     /locus_tag="VFL04_07895"
     CDS             2498..2884
                     /locus_tag="VFL04_07895"
                     /inference="COORDINATES: protein motif:HMM:NF019507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HET7839666.1"
                     /translation="MSQNMTCITCPIGCSLSIEREEGGGILVSGNRCPRGAAYAREEL
                     LSPRRTVTATVRARGHQPGSAPTAADCPRRIPCRSLSPFPRDRIDELLSVLYAMELEL
                     PVERGRILLKDALGTGIDIAVTRTIG"
     gene            2982..3320
                     /locus_tag="VFL04_07900"
     CDS             2982..3320
                     /locus_tag="VFL04_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839667.1"
                     /translation="MSIPSVAAQVIISIIPIVGIVMGSVVVFFHLLWSHKEKLALIAK
                     GLYEQPRIDIDTFSLLAGLLLSCVGAVLSAIFVAMDGFSYPLLGGAIPLAVGVGFLAF
                     FALRRDRTRT"
     gene            3389..3910
                     /locus_tag="VFL04_07905"
     CDS             3389..3910
                     /locus_tag="VFL04_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7839668.1"
                     /translation="MVLEGDREAFSILVQRHGRRVQRIGFSFFRDEDEAADFTQDVFV
                     KAYVALGSFKGRSQFSTWLLRIAYNTAINAKKRRREQLRLECEDEPTGGLGTDEAHLR
                     EESARAVREAVAALPPRQGICLELFFFYNLKYSEISEVTGFPVNTIKSHVFRAKRQLR
                     ERLEGKLEVDGAL"
     gene            3897..4394
                     /locus_tag="VFL04_07910"
     CDS             3897..4394
                     /locus_tag="VFL04_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839669.1"
                     /translation="MVRCEVFLERLDRLDTGQVLGPAMRAHASSCPACAREAARLGLA
                     LEALATAEASAERGDDPLQSRRQRLMVERVMAEIGLMPLPRQDYFAARDWIIAGSVIA
                     LSIALIPFGKDFAELMRLFGTGFSLPLALVLGLVLSSYGTYFVATHIEEVQDFVSRRT
                     RLRKG"
     gene            complement(4482..>4942)
                     /locus_tag="VFL04_07915"
     CDS             complement(4482..>4942)
                     /locus_tag="VFL04_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7839670.1"
                     /translation="GEKMKGLGPEMSPQHWHRPWFDMAQDENADYYPGYSYPPMNIYL
                     TQERSIVFEFAVAGFDEKDISLTFQGDYMVFSARIGVEEIPEDDVRYFKRRLKLKDIE
                     KQKYYVPADKFAQERVKALFKNGILKVTVPPKDEAEGNEGIRIEILKEGD"
BASE COUNT          826 a         1626 c         1648 g          842 t
ORIGIN      
        1 cgaggtccag ggatcgatga tgttcgaccg gctgaaggcc gagctcgatt tccccttcgt
       61 gcgctgcggc atcgtggtcg cggcgatgca cgaggacgag atgcgcggca tcggggccct
      121 gtattcccag gggcttcgga acggggctgt cgggatcgag atgtgctcga gggagcgcct
      181 cctcgagctc gagcccaagc tttcgccgga ctgcgtcggc ggcctctacg ccccggcggg
      241 cggcatcatc gagccctacc gcttcgtctt ctccctcgtg gagtcggcct gcaagaacgg
      301 ggtcaggctc ctcaccggat ggaggctcgt ggccgcgacg aggacgggga accgctggct
      361 gctccgggca gccgatgggc gagagcttgc ggcgagattt gtcgtcaacg ccgctgggct
      421 cttcgccgac gaggtctccc gggccctcgg ggccgaggat ttccggatcc agggccgcaa
      481 gggcgaatac tacctgctgg acaggctcac gaaggccagg cccgaccgcg tcgtcttccc
      541 cgtgccaagc cccatctcca agggcatgct cgtgataccc acggtcgagg ggacggtcct
      601 gatcgggccg acggcgacga aggtcgacga caaggaggac ttcgcgacga cccgagagga
      661 gctcgaggcg atcctcagga gcgcgcggca cctgattcca gcgatctcgg agggcgacgt
      721 gatcaccagc ttcgccggga tcaggtcggt gctcggcgag gacttctaca tcgagctctc
      781 ggcgaaggcc ccggccctcg tccaggtcgc gggcatccag tccccaggcc tgaccgccag
      841 cccggccatc ggtgagtacg tgaaggacct cctcaagaag ccgggcctga ggctcgtcga
      901 gaaggcggac tgggatccct atgtcgagaa ggtccccagg gccaaggaca tggacccctt
      961 cgagctcgat gcccaggcga gggccgatcc cgccttcggc aacatcgtct gccgctgcga
     1021 gcgcgtgacc gaggccgaga tcgtggccgc catccggcgc ggccacacca ccctcgacgg
     1081 gatcaagtac ttcaccaggg cccagatggg ccgctgccag ggcggcttct gcaccctgag
     1141 gatcctgaag atcctcatgc gggagacggg gatgagcccc gaggcgatca ccaaacgcgg
     1201 aggccagagc agggtcctcg ggggcgagct atgaaggagc gcagcttcga cgccgtcgtc
     1261 gtggggggag gggcggccgg catggctgcc gccctggccc tcgatgcccg tggcaagcgc
     1321 gtcgccatcg tcgacaggga ggcggggctg ggcggcatcc tggtccagtg cattcacaac
     1381 ggtttcgggc tcatcgagtt caacgaggag ctctccggac ccgagttcgc ggagcgcctc
     1441 gaggaggtgg tggcgaagac gcgcatcgcg gtcttccgcg gctccacagt gttcgagctc
     1501 gaggcctcgg gcgatgagaa gaccgtggtc tgcgtctccg cggccttggg ggtactgcgc
     1561 ctccactgcc gagccgtcgt gctcgcgatg ggatgccggg agcgcaaccg cggcaacatc
     1621 aggatccccg gcacgaggcc ggcgggggtc ttcaccgccg gcctggccca gcgcctcgtg
     1681 aacgtcgagg gctacctccc cggcaaggat gtcgtcatca tcggctcggg ggacatcggc
     1741 ctcatcatgg cccgccgcat gagctgggtg ggctgcaggg tgcatgccgt cgtggagatc
     1801 ctcccctatc cctcgggcct cacccggaac atcgtgcagt gcctccacga tgtcggcatc
     1861 cccctctatc tctcccacct gaccacctgc atacgtggac atgacagggt cgaaggcgtc
     1921 caggtaaccc cattcgtgaa cggcgccctc gtgcccgaga aggcattcga catcccctgc
     1981 gacaccgtcc tcctctcggt gggcctcgtg cccgagaacg agctcacgaa gaaggcgggc
     2041 atcgacatga acccagccac gaacgggccg gcggtggact cgactatgat gacgagcctg
     2101 gccggggtct tcgcctgcgg caacgtcctc cacgtgcacg acctcgtgga ctacgtcgtc
     2161 gaggagtcgc ggagggccgg ctccttcgcc gccgactggc tggacgggac aaggccgggc
     2221 aaggaggcga ggatcaaggt gggtcccaac gtgcgctacg tcagcccggg ccgcatcgac
     2281 ctggcgaggg agaacaaggt ctatctccgc agcctcgtcg tgaagaatga cgccactctc
     2341 gaggtgaggc tcggcaaccg actcatccgc agcatcagga agaaccatgt ccagccctcg
     2401 gagatgatct ccctgagcct gggggggaag gagctggagc ccgaagcctc cacggcggag
     2461 tccgtcatcg agatcgcgat ccgctgaagg agggggcatg agccagaaca tgacctgcat
     2521 aacctgtccg atcggctgta gcctgagcat cgagcgagag gaggggggag ggatcctcgt
     2581 gagcggaaac cgctgccccc ggggcgcggc gtatgccagg gaggagctgc tctcgccccg
     2641 gcgaacggtg acggcaacag tccgcgcccg cggccaccag cccggctcgg cccccacagc
     2701 cgcggattgc ccgcgccgga tcccctgcag gagcctttcg ccctttcccc gcgaccgcat
     2761 cgacgagctc ctctcggtgc tctatgccat ggagctcgag ctgcccgtcg agcggggccg
     2821 catcctcctc aaggacgccc ttggcacggg catcgacatc gccgtgacgc gcacgatagg
     2881 ctgattgccc aaagcccgtg gccggggggc aacctttttg gcccggcccc tgtctctatc
     2941 tgtacgcacg aaacaccagg ggacaaaagg agagccagtc gatgtccatc ccatccgtcg
     3001 cggcgcaggt gatcatctcg atcatcccca ttgtggggat cgtcatgggc agcgtcgtcg
     3061 tcttctttca cctattgtgg agccacaagg agaagctggc cctcatcgcg aaaggcctct
     3121 atgagcagcc ccgcattgat atcgacacct tcagcctgct cgcgggactc ctcctgagct
     3181 gcgtcggcgc cgtgctctcg gccatcttcg tcgccatgga cggattctcc taccctctcc
     3241 tcggcggggc gattcccctg gcggtcggcg ttggcttcct cgcctttttc gcgctgcgga
     3301 gggaccggac gaggacatga tccggcagcg gccgcaaacc gcggagagcc tgccggttcg
     3361 ggatccgagc gatgaggagg ccatcgccat ggtccttgaa ggcgaccgcg aggccttctc
     3421 catcctggtc cagcgccacg gcaggagggt ccagaggatc ggcttcagct tcttcaggga
     3481 tgaggacgag gccgcagact tcacccagga cgtcttcgtg aaggcctatg tggccctcgg
     3541 tagcttcaaa ggcagatccc agttcagtac ctggctcctc aggatcgcct acaacacggc
     3601 gatcaacgcg aagaaacgac gcagggagca gcttcggctc gagtgcgagg acgagccgac
     3661 ggggggcctg gggacggacg aggcccatct ccgcgaggag tcggccaggg ccgtgcgcga
     3721 ggctgtggcc gccctgcccc ccagacaggg aatttgtctg gagctcttct tcttttacaa
     3781 tttgaagtac tcggagatca gcgaggtcac cgggttcccg gtgaacacta tcaagtccca
     3841 cgtcttccgc gcgaaacggc agctgcggga gcgcctcgaa gggaaactgg aggtcgatgg
     3901 tgcgttgtga agtgtttctt gagcgcctgg accgcctcga cacggggcag gtcctgggcc
     3961 cggcgatgcg ggcccacgcc tcctcgtgcc ccgcctgcgc cagggaggcg gcgaggctgg
     4021 ggctggccct cgaggccctc gcgacggccg aggcttcggc ggagcgcggc gacgatcccc
     4081 tgcagagccg gagacagagg ctcatggtag agcgcgtgat ggccgagatc gggctcatgc
     4141 cccttcctcg gcaggactac ttcgcggcgc gcgactggat catcgccggg tcggtcatcg
     4201 ccctctcgat agccctcatc cccttcggca aggacttcgc ggagctcatg cggctcttcg
     4261 gaacgggctt ctcgctcccg ctcgcccttg tcctgggcct cgtcctgagc tcctatggca
     4321 cctacttcgt cgccacccac atcgaagagg tccaggactt cgtgtcccgc aggacccgtc
     4381 tccgcaaggg ctgatctcga aaacggcgcc gcttcccgca agggcggcgg cgccggccga
     4441 ggccgccgca aggggctgcg gctgcccggg agaccggaac cctagtcgcc ctccttcaga
     4501 atctcgatcc tgatcccctc gttgccttcg gcctcgtcct tgggcggaac cgtgaccttg
     4561 aggatgccgt tcttgaacag ggccttgacg cgctcctgcg cgaacttgtc agcggggaca
     4621 tagtacttct gcttctcgat gtccttgagc ttgagccttc gcttgaagta gcggacgtcg
     4681 tcctcgggga tctcctcgac cccgatcctg gccgagaaga ccatgtagtc tccctggaag
     4741 gtgaggctta tgtccttctc gtcgaagccg gcgacggcga actcgaagac aatgctgcgc
     4801 tcttgggtga gatagatgtt catgggcgga taggaatagc cgggataata gtcggcgttc
     4861 tcgtcctggg ccatgtcgaa ccaggggcgg tgccagtgtt gggggctcat ctcgggccct
     4921 aggcccttca ttttctcgcc ga
//
LOCUS       DASSQV010000451         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1350644, whole genome shotgun sequence.
ACCESSION   DASSQV010000451 DASSQV010000000
VERSION     DASSQV010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350644"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VFL04_07920"
     CDS             <1..1157
                     /locus_tag="VFL04_07920"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HET7839671.1"
                     /translation="QGSRWAGAGADPRGEAPQRRALPGRLGLKAMEGDIVIRGSARVS
                     TLRFAALEEAAARLDPARSVIVTDAEIARLHGGRFPPFPLALVERGEAAKSLSSLEAL
                     YARFLELGLGRDSTVLAIGGGSVSDLAGFAASTWLRGVDFRFVPSTLLAMVDASVGGK
                     NGIDFRGLKNLIGSFGQPGLVVFDTSLLDTLGQGGFASGMAEAIKHALIEGEEHLAVL
                     ESLGTEASAIGRADRERLVRLSVTTKAAIVGSDEREAGGRRRLNLGHTIGHAVEAVTA
                     LPHGHSVAAGLASALLIAERRGLLGGGLRARVVRLLSAWGLPSSLAAAASLAGVADGP
                     GYRQSLVRALAADKKRTGGQIQFAMPEAPGHVSILPIHLEEIGAFVLEAP"
     gene            1154..2155
                     /locus_tag="VFL04_07925"
     CDS             1154..2155
                     /locus_tag="VFL04_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET7839672.1"
                     /translation="MNRFGNAFRVEIFGESHGAGAGVIVDGCPPGLEIGPEDLEQDLA
                     RRRSGAAGTTPRREADLPVFLSGVYQGRSTGSPICILFRNEDTRSSDYAAFATVYRPG
                     HADFTASVRYRGFADPRGSGHFSGRITVGLVAAGAIAKRILGGVIFETRLLEAGGSAD
                     VAAAVAAASEAGDSVGGLLELRVRGLPAGLGEPFFDSIEGLIAHAVFAVPGIRGVEFG
                     DGFAAARMRGSQHNDSFVERSGRTATNHAGGANGGISNGNELLFRVAVKPTSTIAAPQ
                     RSLDFATGEEAELAAGGRHDACIAIRAAVAVEAAAAIAIADLALAARGYDTAKRGEP"
     gene            2152..>3156
                     /locus_tag="VFL04_07930"
     CDS             2152..>3156
                     /locus_tag="VFL04_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HET7839673.1"
                     /translation="MTLKDLREDIDRIDARLLALLNERLEKALMTKSFKDRVVDEGRE
                     LQVLERARRSARCLAGPEFSVELFRSIMAESRAVQTRGLETIGFQGEHGAYSEMAAKA
                     WSGKAAAMPCREFVDVFDAVQEGRLDYGIVPVENTLGGLVGPVNSILVYSDLAIVAAV
                     DMPVSHCLLSLPGADHREIRAAYSHTQALAQCRRFLARNRLEARPWFDTAGAARMLAE
                     ERPAGVAVVASRFAAELYGLEIIKDGIQDSEDNRTRFFVLARRSPAEPEAAPPTGAGA
                     SALKCSAVFFTEDKAGSLFGTLEIFARAGINLTRIESVPNRPGDYAIFIDFEGSEREP
                     R"
BASE COUNT          424 a         1200 c         1089 g          443 t
ORIGIN      
        1 ctcagggcag ccgatgggcc ggggccgggg ccgatccccg cggtgaggcg ccccagcggc
       61 gagcgcttcc cggacgcctc ggactgaagg ccatggaggg cgacatcgtc atccgcggct
      121 cggcgcgggt ctccacgctc cgcttcgccg ccctcgagga ggcggccgcg cgcctcgatc
      181 cggcgcgctc ggtcatcgtc accgacgccg agatcgcccg cctccacggc ggccgcttcc
      241 ctccattccc cctcgcgctg gtcgagcgcg gcgaggcggc gaagagcctc tcctcgctcg
      301 aggccctcta cgcgcgtttc ctcgagctgg gcctgggcag ggactcgacc gtcctcgcca
      361 tcggcggcgg ctcggtgtcc gacctcgcgg gcttcgccgc ctcgacctgg ctccggggcg
      421 tggacttccg cttcgtcccc tcgaccctcc tcgcgatggt cgacgcctcg gtgggcggca
      481 agaacgggat cgacttccgc ggactcaaga acctcatagg ctccttcggc cagcccggcc
      541 tcgtggtctt cgacacatcc ctcctcgaca ccctcggcca gggcgggttc gcctcgggca
      601 tggccgaggc cataaagcac gccctgatcg agggggagga gcacctcgcc gtcctcgagt
      661 ccctggggac cgaggcctcg gcgatcggcc gagccgaccg cgagcgcctg gtaaggctct
      721 cggtcacgac gaaggcggcc atcgtgggct cagacgagag ggaggcgggt gggcgccgcc
      781 ggctcaacct cgggcacacg atcggccacg ctgtcgaggc ggtcaccgcc ctcccccacg
      841 gccactcggt cgctgctggc ctggcctcgg ccctgctcat cgccgagcgc cgcggcctcc
      901 tgggcggggg cctgagggca cgcgttgtgc ggctcctctc ggcctggggc ctgcccagct
      961 ccctcgccgc cgcggcctcg ctcgctggcg tcgccgacgg gcccggctac cggcagtccc
     1021 tcgtgagggc cctcgccgcc gacaagaagc ggaccggagg tcagatacaa ttcgccatgc
     1081 ccgaggcccc aggccacgtg agcatactgc cgatccacct cgaggagatc ggcgcgttcg
     1141 tgttggaggc cccatgaacc gcttcggcaa cgccttccgc gtcgagatct tcggcgagtc
     1201 ccacggcgcc ggcgccgggg tcatcgtcga cggctgcccc cccggcctcg agatcggccc
     1261 cgaggacctc gagcaggacc tcgcgcggag gcgctcgggg gccgcgggca cgaccccgcg
     1321 gcgcgaggcc gaccttcccg tcttcctctc cggggtctac caggggcgga gcacgggcag
     1381 cccgatctgc attctcttcc gcaacgagga cacgaggagc tcggactacg cggccttcgc
     1441 cacggtctac cgccccggcc acgccgactt cacagcctcg gtgcgctacc gcggcttcgc
     1501 cgaccccagg ggctcgggcc acttctcggg ccgcatcacc gtcggccttg tcgccgctgg
     1561 ggccatagcc aagaggatcc tcggaggcgt gatcttcgag acgcgcctcc tcgaggcggg
     1621 gggcagcgcc gacgtggccg ccgccgtcgc cgccgcctcc gaggccggcg actcggtcgg
     1681 gggcctcctc gagcttcggg tgcggggcct ccccgccggg ctcggcgagc ccttcttcga
     1741 ctcgatcgag ggcctcatag cccacgcggt cttcgccgtg cccggcatca ggggcgtcga
     1801 gttcggcgac ggcttcgcgg ccgcgaggat gcgcggctcg cagcacaacg acagcttcgt
     1861 ggagcgctcg ggccgcaccg cgacgaacca cgccggcggg gccaacgggg gcatctcgaa
     1921 cgggaacgag ctcctgttcc gcgtggcggt caagccgacc tccacgatcg ccgcccccca
     1981 gcggagcctg gacttcgcca cgggcgagga ggccgagctc gccgccgggg gcaggcacga
     2041 cgcctgcatc gcgatcaggg ccgccgtcgc cgtcgaggct gcagcggcca tcgccatcgc
     2101 cgaccttgcc ctggccgcgc gaggctacga cacagcgaag aggggggagc catgacgctc
     2161 aaggacctgc gcgaggacat cgacaggatc gacgcgaggc tgctcgcgct cctcaacgag
     2221 aggctggaga aggccctcat gacgaagtcc ttcaaggaca gggtcgtcga cgagggccgc
     2281 gagctccagg tcctcgagcg cgcccgccga tcggcccgct gtctcgccgg ccccgagttc
     2341 tcggtcgagc tcttccggag catcatggcc gagagcaggg cggtccagac ccgcggcctc
     2401 gagaccatcg gcttccaggg cgagcacggg gcctacagcg agatggccgc caaggcctgg
     2461 agcgggaagg ccgccgccat gccctgccgg gagttcgtcg acgtcttcga cgctgtgcag
     2521 gaggggcgcc tggactacgg catcgtcccc gtcgagaaca ccctcggcgg cctcgtgggg
     2581 ccggtgaact ccatcctcgt gtactccgac ctcgcgatcg tcgcggccgt ggacatgccc
     2641 gtctcccact gtctcctctc gctccccggg gccgaccacc gcgagatccg ggcggcctac
     2701 tcccacaccc aggccctggc ccagtgccgc cgcttcctcg cgcgcaaccg gctcgaggcc
     2761 aggccctggt tcgacacggc tggggcagcg aggatgctgg ccgaggagag gccggccggg
     2821 gtggccgtgg tcgcgagccg tttcgcggcc gagctctacg gcctcgagat catcaaggac
     2881 gggatccagg actctgagga caaccgcacc cgcttcttcg tcctcgccag acgctccccg
     2941 gcggagcccg aggcggcccc gcccaccggg gccggcgcgt cggcgctcaa gtgctcggcc
     3001 gtgttcttca ccgaggacaa ggccggctcc ctcttcggga cccttgagat cttcgcccgg
     3061 gccggcatca acctcaccag gatcgagtcg gtccccaaca ggccggggga ctacgcgatc
     3121 ttcatcgact tcgagggctc ggagcgcgag ccgcgg
//
LOCUS       DASSQV010000452         2894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_106209, whole genome shotgun sequence.
ACCESSION   DASSQV010000452 DASSQV010000000
VERSION     DASSQV010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106209"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..620)
                     /locus_tag="VFL04_07935"
     CDS             complement(93..620)
                     /locus_tag="VFL04_07935"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7839674.1"
                     /translation="MDARREIEPATMKDRTRVERKTERELVTTRAFRAPARIVFRAWT
                     TPELFRRWWVPKSFGMTLLSFEADFRAGGRYRLLFSHPAAPEPMAFFGTYLEVTPHSR
                     LVWTNEEGGESGQLTTVSFEERGGETLVVVRDLYPSKEALDEAIASEATGALPEQFEQ
                     LDELLVGLVADIGQA"
     gene            complement(547..963)
                     /locus_tag="VFL04_07940"
     CDS             complement(547..963)
                     /locus_tag="VFL04_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7839675.1"
                     /translation="MVQYRQTRFDASFAALSDATRRGVLERLGRADASITDLAESFQM
                     SLTGMKKHVGVLEQAGLVTTEKVGRVRTCRLGRRRLEEEAAWLEKYRQLWDARFDELD
                     KVVAELDQKEKTDGRKKRDRARHHEGPDEGGTEDRA"
     gene            complement(1082..2143)
                     /locus_tag="VFL04_07945"
     CDS             complement(1082..2143)
                     /locus_tag="VFL04_07945"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HET7839676.1"
                     /translation="MSTSAAAPKSGKRSWPARNLPGLLPVAAVSLAALLLASTRLFSA
                     TLPLSPLILAILIGLACGNVLKLPASTKEGIRFASKKVLRLAIIFLGFKLSVAQVLAI
                     GPQALASILVASTSTLLFTVWLGSRMGVPRSRSLLLGAGVSICGASAVAAVSGVIGGE
                     EEDAAFAISAITLYGTVAMFLYPVMRALLGIPDLFYSMWTGESVHEVAQVAAASAVVA
                     DQLRGLAATVKMIRVLLIVPLTLILSVMALRGEAPGRKAGAANAGLRPGARVSVPWFA
                     LLFFAMVLVNSFAPLTAGLRGGLVTFDTWIMTAAMAGLGLDISIASLRKIGPRAALLG
                     ALSSLFISAASAALSLILS"
     gene            complement(2197..2400)
                     /locus_tag="VFL04_07950"
     CDS             complement(2197..2400)
                     /locus_tag="VFL04_07950"
                     /inference="COORDINATES: protein motif:HMM:NF036547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7839677.1"
                     /translation="MADRTGTVTPRPPPAPAYGDAASLDSLERAAIERALAKWEGNRS
                     KAAAELGISRRTIQNKIKRFGLD"
     gene            complement(2479..>2894)
                     /locus_tag="VFL04_07955"
     CDS             complement(2479..>2894)
                     /locus_tag="VFL04_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839678.1"
                     /translation="RLEDYYFSFDRNETIERHFYTDNLVVAGRTAKAIEGGRTIPLTR
                     TLFDEGCLIFKAPASRRFSLANRLNARSLTLSTEGAPFLGLWSKPKGAPFVCIEPWHG
                     IPDSTDRSGELTLKEGILGLDPGESFATGYRVEIG"
BASE COUNT          458 a          939 c         1035 g          462 t
ORIGIN      
        1 cgtgaggcgc tgtccccaga aaaaacgtcc gacccccgcg cgggggtcgg acgttttttc
       61 tggggacagc gcctcacgcc actgccgagg aatcaggcct ggccaatgtc tgcgaccagg
      121 ccgacgagca gctcgtccag ctgctcgaac tgctcgggca gggcccccgt cgcttctgag
      181 gcgatggcct cgtcgagagc ctccttcgag ggatataggt cgcgcacgac caccagggtc
      241 tcgccgcctc tctcctcgaa gctgaccgtt gtgagctgcc cactctcgcc gccttcttca
      301 ttggtccaga cgaggcgcga gtgcggcgtc acttcgaggt aggtgccgaa gaaggccatg
      361 ggctccgggg ccgcggggtg gctgaacagg agacggtacc ggcctccggc acgaaaatcc
      421 gcctcgaagg agagcagggt catgccgaag gacttcggca cccaccaccg ccggaacagc
      481 tcgggcgtgg tccaggcccg gaacacgatg cgcgccggag cgcgaaacgc ccgcgtcgtg
      541 acgagctcac gctcggtctt ccgttccacc ctcgtccggt ccttcatggt ggcgggctct
      601 atctctcttc ttgcgtccat cggtcttctc cttctggtcc agctctgcga ctaccttgtc
      661 cagctcatcg aagcgcgcgt cccagagctg acggtacttc tcgagccagg ccgcctcttc
      721 ctccagtcgg cgccggccga gcctgcaggt ccgcacgcgg ccgaccttct ccgtggtgac
      781 gagccccgcc tgctccagga ccccgacgtg ctttttcatg cccgtcaggc tcatctggaa
      841 gctctcggcg aggtccgtga tggaagcgtc ggcccgtccg agccgctcca gaacgccgcg
      901 ccgggtggcg tccgaaagcg cggcgaagga ggcatcgaag cgggtttggc gatactgaac
      961 catgtggttc agtatactgc tcgcggacgg cataggtcaa accctgcccc gagttcgggc
     1021 tggattgcct acgaggtcga ggcacgggaa gggtccgggt ggagcccccc gcggtgacta
     1081 cctacgagag gatgagactc agggcggcgc tcgccgccga aatgaacagc gaggacaggg
     1141 ccccgaggag ggcggccctc gggccgatct tcctcaggct ggcgatgctt atgtcgagcc
     1201 cgagcccggc catcgccgcg gtcatgatcc aggtgtcgaa ggtcacgagg ccgccgcgca
     1261 gacccgcggt gagcggggcg aaggagttca cgaggaccat ggcgaagaag aggagggcga
     1321 accagggcac gctcacgcgg gcccccggtc ttaggcccgc attcgccgcg cccgccttcc
     1381 gtccgggcgc ctcgcccctg agggccatca ccgagaggat gagggtcagg ggcacgatca
     1441 ggaggacgcg gatcatcttc acggtcgcgg cgaggccgcg gagctggtcg gccacgaccg
     1501 cgctcgccgc ggccacctgg gccacctcgt ggaccgactc cccggtccac atcgagtaga
     1561 agaggtcggg gatgccgagg agggccctca tgacgggata caggaacatc gccacggtgc
     1621 cgtagagagt gatggcgctg atggcgaagg ccgcgtcctc ctcctcaccg ccgatgacgc
     1681 cgctgacggc tgccaccgcc gaggcgccgc agatcgagac gccggcgccc aggaggaggg
     1741 acctggacct ggggactccc atccggctcc ccagccagac ggtgaagaga agggtcgagg
     1801 tcgaggccac aaggatggag gcgagggcct ggggcccgat ggcgaggacc tgggccacgc
     1861 tcagcttgaa gccgaggaag atgatcgcga ggcggaggac cttcttcgag gcgaaacgga
     1921 ttccctcctt cgtcgaagcg ggcagtttca gcacatttcc gcaggcgagc ccgatgagga
     1981 tcgccaggat gagggggctc agggggaggg tcgcggagaa gagccgcgtc gatgcaagga
     2041 ggagggcggc gaggctgacc gcggcgacgg gaaggaggcc gggcaggttt ctggcgggcc
     2101 aggatctctt gcccgatttg ggggctgctg cgctggtgga cacggtgggg ctacctcggg
     2161 ggagtggtgg cgggagtcta gccgatccgg gcgatcctag tcgaggccga agcgcttgat
     2221 cttgttctgg atcgtcctgc ggctgatgcc gagttcggcg gcggccttgc ttcggttgcc
     2281 ctcccatttc gcgagggcgc gctcgatcgc agcccgctcg aggctgtcga gcgaggcggc
     2341 gtccccgtag gcgggggcag gcggcggtct cggggtgact gtccccgtcc ggtccgccat
     2401 cgagccgcca tccggggcgg cccgcccggg gcacctgccc ccggcggagc cggtccggca
     2461 cctgggcgcc gggaaggcct agcctatctc gaccctgtac cccgtcgcga agctctcccc
     2521 gggatcgagc ccgaggatgc cctcctttag ggtcagctcc ccgctgcggt cggtcgagtc
     2581 ggggatcccg tgccagggct cgatgcatac gaagggggcg cccttcggct ttgaccagag
     2641 gccgaggaag ggggcgccct cggtgctcag ggtcaggctg cgggcgttca ggcggttcgc
     2701 gaggctgaag cgccttgatg ccggggcttt gaagatgagg cagccttcgt cgaacagggt
     2761 ccttgtcagg ggtatcgtcc gccccccctc gatggccttg gccgtcctgc cggccacgac
     2821 caggttgtcg gtgtagaagt gccgctcgat ggtctcgttg cggtcgaagg agaaatagta
     2881 gtcctcgagc ctcc
//
LOCUS       DASSQV010000453         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2470006, whole genome shotgun sequence.
ACCESSION   DASSQV010000453 DASSQV010000000
VERSION     DASSQV010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2470006"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFL04_07960"
     CDS             <1..604
                     /locus_tag="VFL04_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839679.1"
                     /translation="GSTGAAGSSGAGVSGGTGTVTYHGTEMGNAIDTTFGGASGKVGR
                     NLYAPVYMFMPPPQAISDEMYNRIPAQANGYYSAEERKRAFRDYYARTGDTWRLKKTV
                     PLNQRNPLWEILQDAGYDPATADFKAGRQLKPVVLDFVIAPASPGKRPELVDLQLVSS
                     SGYAEIDEAVMYGFRQATYFNNTGNAISGRFTYGFDQGGK"
     gene            604..2025
                     /locus_tag="VFL04_07965"
     CDS             604..2025
                     /locus_tag="VFL04_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7839680.1"
                     /translation="MRLQPKVLSIFAAALVLMLGFAVLAFPSLVSRVSEAQLFSLART
                     LGAYVVHDLETQPFAGDTAAFEKALDQRFEFAQRLGEGTGDYLVRSVALIGPDFKVEV
                     GHPNSEIGADYSGHADVREEMATGNAGDQRIALESSRPGGPVTDADILTKVRLADGDS
                     RVLEIKLDFSGTLAMLTRQYARLQAAALGLIAAGLIAVTIALASLLRGTVLRPLLRIS
                     KAMELVGKGDLAVSANVRSRDEIGQMGEHFDTMVAGLRERFELERYVSRSTLGAARDP
                     AARDRAEGTGSAVERRRLAVLFSDVRGFTAYSECADPARVVSVLNLILGAQEEIIAKA
                     GGEVDKFVGDEVMAVFERPALAIAAALVIRSKMARISGQIDGLSLGYGIHVGELVQGD
                     IGSPARMDHTVIGDTVNTASRLQAASGAGQIIVSAEVAADPEVASRFVLDELGPLKVK
                     GKEQPLSTFTVRGAREPAKDEIG"
BASE COUNT          340 a          727 c          699 g          320 t
ORIGIN      
        1 gggatcgacg ggagccgccg ggtcctcagg cgccggggta agcggaggca cgggcaccgt
       61 gacctaccac ggcaccgaga tgggcaacgc gatcgacacg acctttggcg gggcttcggg
      121 caaggtgggc aggaacctct atgccccggt ctacatgttc atgcccccgc cccaggcgat
      181 cagcgacgag atgtacaacc ggatccccgc ccaggcgaac ggctactatt cggctgagga
      241 gcgaaaaagg gccttcaggg actactacgc gcgcacgggc gacacctgga ggctgaagaa
      301 gaccgtcccc ctcaaccaga ggaacccgct ctgggagatc ctccaggacg ctggctacga
      361 tccagccacg gccgacttca aggccggcag acagctcaag cccgtcgtcc tcgacttcgt
      421 cattgccccg gcttcacccg gcaagcggcc cgagctcgtc gacctccagc tcgtctcctc
      481 ctcgggctac gccgagatcg acgaggcggt gatgtacggc tttcgccagg ctacctattt
      541 caacaacacg ggcaacgcca tctcgggcag gttcacctat ggcttcgacc aagggggcaa
      601 gtgatgaggc tccagcccaa ggtcctgtcc atcttcgctg cagccctcgt cctcatgctc
      661 ggcttcgccg tcctggcctt cccgagcctg gtgtcgaggg tatcggaggc ccagctcttc
      721 tccctcgccc gcaccctggg ggcctatgtg gtccacgacc tcgagaccca gcccttcgcc
      781 ggggacaccg cggcgttcga gaaggccctt gaccagcgtt tcgagtttgc ccagaggctc
      841 ggcgagggca cgggcgacta cctcgtgcgg agcgtggccc tgatcgggcc tgacttcaag
      901 gtcgaggtgg ggcatcccaa ctccgagatc ggggccgact actcgggcca cgccgacgtc
      961 cgcgaggaga tggccacggg caatgccggg gaccagcgca tcgcccttga gtcctcgagg
     1021 ccgggcggcc ccgtgaccga cgccgacatc ctgaccaagg tccgcttggc cgacggggac
     1081 tcgagggtcc tcgagatcaa gctggacttc agcgggaccc tggccatgct gacccggcag
     1141 tacgccaggc tccaggccgc ggccctcggc ctcatcgcgg cgggcctcat cgccgtgacc
     1201 atcgccctcg catccctgct ccgcggcacc gtcctccgcc cgcttctgcg catctcgaag
     1261 gccatggagc tcgtggggaa gggcgacctt gctgtttcgg ccaacgtgcg atcgagggac
     1321 gagatcggcc agatgggcga gcacttcgac acgatggtgg ccggcctgcg ggagcgcttc
     1381 gagctcgagc gctatgtctc gcgctccacc ctcggggccg cccgggaccc ggcggccagg
     1441 gaccgggccg aagggaccgg ctcggcggtc gagcgcaggc gcctcgccgt cctcttctcc
     1501 gacgtgcggg gcttcaccgc ctactccgag tgcgccgacc cggcgcgggt agtgtccgtc
     1561 ctcaacctga tcctgggcgc ccaggaggag atcatcgcga aggccggggg cgaggtggac
     1621 aagttcgtcg gcgacgaggt catggccgtc ttcgagcggc cggccctggc gatagccgcg
     1681 gccctcgtca tacgctcaaa gatggcgagg atctcgggcc agatcgacgg cctctcgctg
     1741 ggctacggga tccacgtcgg cgagctcgtc cagggggaca tcggctcgcc ggcgcgcatg
     1801 gaccacaccg tcatcggcga caccgtgaac acggcgtccc ggctccaggc ggcctcgggg
     1861 gcgggacaga tcatcgtctc ggccgaggtg gccgccgacc ccgaggtggc ctcgcgtttc
     1921 gtcctcgacg agctcggccc cctcaaggtc aagggaaagg agcagcccct ctcgaccttc
     1981 acggtgagag gggcccgaga gccggcgaag gacgagatcg gctaacgcgt gtagtactcg
     2041 acgaccaggt gaatcttgcc ggggaccgag gcctcctggg cgttcg
//
LOCUS       DASSQV010000454         4316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_355311, whole genome shotgun sequence.
ACCESSION   DASSQV010000454 DASSQV010000000
VERSION     DASSQV010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355311"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="VFL04_07970"
     CDS             complement(<1..1825)
                     /locus_tag="VFL04_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET7839681.1"
                     /translation="MRFAHDDHSLSGLAVPLSSLRSASSPGCGEYPDLAAMGDLASSW
                     GMGLVQLLPVNDTGSQTSPYSALSAFALHPLYIRITELPEIRPAAAKSMRAEALALVD
                     RFRDAEAVPHEELLVAKLELLWRVWEHGGQASRADLDRWIQANPWVRAYAAFVELKRR
                     NGGKPWWEWPSLVEPSPAELEALWSEPDLASALAFWAWLQMRAEGQFQAAASHLASLG
                     VELMGDIPILMNRDSADVWSRRGVFDLDLVAGAPPDMYAELGQNWGFPIYDWKALEEE
                     GYGFWVDRLREADKYYSCYRIDHVLGFFRIWSIGQRASSGYLGRFVPEEHITRAELEG
                     LGFDSGRIRWLSRPHVRLSSIEAACGPERDAVIAAHFDRIGSEELFLFKAGIEGELDF
                     GEAATRSGRPLSGPCLDFLGKAWRDRALYEFEPGCFAPAWRYYEASSWPSLSEAERGS
                     LEALFAGKRAASERLWADTGRRLLGILCEAVPMLPCAEDLGAVPDCVPLVLGELGILG
                     LRVLRWTRLWRLPGQPYQPLSEYPRLTVATPSVHDSSSLRGWWQTEANREQVWSFFED
                     CLGRRLGPCPSELDPHGVAVALEAIARSKSRFAVYPIQDLIA"
     gene            complement(1905..3290)
                     /gene="radA"
                     /locus_tag="VFL04_07975"
     CDS             complement(1905..3290)
                     /gene="radA"
                     /locus_tag="VFL04_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET7839682.1"
                     /translation="MTAKKKSLVYRCSACGHSESKWLGRCPECGQWNTLRETALAQAR
                     AERGGETFPLPIASIEAGEETRIPTGSGEMDRVLGGGIMRGSVVLLGGEPGIGKSTLL
                     LQILAKAATKGRLLYISGEESPAQIKLRAERLGCIRDGLEVFCSGDLGLVRATLDAVR
                     PTIAVVDSIQTLRAEEAGAVPGTANQIKYCAMELTSWAKEHDSAIILVAHVTKEGIIA
                     GPKAAEHLVDAVLGFEQGEGDMRVLRASKNRFGSAEEVGLYRMGEAGLEEVSDAAGLF
                     LVHRDEGMPAGAAVAPVHEGSRILLVEIQALTVPAKAGLTRVYSERVDAARVSRIAAV
                     LEKHTGARFSDQDLYVNVAGGIRLTEPGIDLALACALYSARSGVPLPLQVALAGELSL
                     AGEVRPVRQMRRRAKAALALGFSRLVGPLGETEARRGERGAEAAEWKGVRTVKEALKA
                     VFGSGTPGREA"
     gene            3405..3809
                     /locus_tag="VFL04_07980"
     CDS             3405..3809
                     /locus_tag="VFL04_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839683.1"
                     /translation="MAQRSASDFGSIFFLQLALGAFFLALGIMGVTDYQHSTRGVMSL
                     FAKNSALKLTTAIIELAMGGVLLLGLVFSVSSALARILGIVLFLLWAVYIVMNFIAKD
                     IFEPSFVPWLYNISWNSVILVALWTVGTRYRA"
     gene            complement(3806..>4316)
                     /locus_tag="VFL04_07985"
     CDS             complement(3806..>4316)
                     /locus_tag="VFL04_07985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7839684.1"
                     /translation="LEATKRRVDAMAFVHEEIYTEPRMDGVRLGAFLSRFAGEIQQAE
                     GGLGRRRIEVDAHDIRLDIQCAVAIGIITAEVVANACRHAFVGRSGGSIWVHARAEEG
                     RTTIEVIDDGVGIPPGLEAGPGLGLTLVRSLASQLNATVLLERRETGGTRFALSVPAR
                     SGFAGQRAC"
BASE COUNT          642 a         1497 c         1518 g          659 t
ORIGIN      
        1 tggcgatgag gtcctggatc gggtagacag cgaagcgcga tttggaccgc gcgatggcct
       61 caagggcgac tgccacccca tggggatcga gctcagaagg gcaggggccg agcctcctgc
      121 cgaggcagtc ctcgaagaag gaccagacct gctcgcggtt ggcctcggtc tgccaccatc
      181 ccctgagcga tgaggagtcg tggaccgagg gggtggccac cgtgagccgc gggtattcgg
      241 acaggggctg gtagggctgg cccgggagcc gccagagcct ggtccagcgc aggacgcgga
      301 ggcccaggat cccgagttcc ccaaggacga ggggaacgca gtcgggcacc gcgcccaggt
      361 cctcggcgca ggggagcatc gggacggcct cgcagaggat gccgaggagc cttctgccgg
      421 tgtcggccca gagccgctcc gaggccgcgc gcttgcccgc gaagagggcc tcgaggctcc
      481 cccgctcggc ctctgagagg ctcggccatg aggaggcctc gtagtagcgc caggccggtg
      541 cgaagcagcc gggctcgaac tcgtagaggg cgcggtccct ccaggccttg ccgaggaagt
      601 cgaggcaggg gccggagagg ggccgtcccg agcgcgtggc ggcctcgccg aagtcgagct
      661 ccccctcgat cccggccttg aagaggaaga gctcctcgct gccgatccga tcgaagtgcg
      721 ccgctatgac ggcgtcccgc tccgggccgc aggcggcctc gatcgaggag agccggacat
      781 ggggcctgct cagccagcgg atcctgcctg agtcgaagcc gaggccctcg agctcggcgc
      841 gcgtgatgtg ctcttcgggc acgaaacgcc cgaggtagcc gctcgaggcg cgttgcccga
      901 tggaccagat ccggaagaag ccgagcacgt ggtcgatccg gtagcaggag tagtacttgt
      961 cggcctcccg cagccggtcg acccagaagc cgtagccttc ctcctcgagg gccttccagt
     1021 cgtagatcgg gaagccccag ttctggccga gctcggcgta catgtcgggg ggggccccgg
     1081 ccacgaggtc gaggtcgaag acccccctcc ggctccacac gtcggcggag tcccggttca
     1141 tcaggatggg gatgtcgccc atgagctcga cgccgagcga ggcgaggtgg gaggccgcgg
     1201 cctggaactg gccctcggcc cgcatctgga gccaggccca gaaggccagg gccgatgcca
     1261 agtccggttc cgaccagagc gcctcgagct cggccggcga gggctcgacg aggcttggcc
     1321 attcccacca gggcttgcca ccgttcctcc gcttgagctc cacgaaggcc gcgtaggccc
     1381 tgacccaggg gttggcctgg atccagcggt cgaggtcggc cctcgaggcc tggcctccgt
     1441 gctcccagac ccgccatagc agctcgagct tcgccacgag gagctcctcg tgcgggacgg
     1501 cctcggcgtc gcggaaacga tcgacgaggg ccagggcctc ggccctcatc gacttggcgg
     1561 cggcgggcct gatctcgggg agctcggtga tcctgatgta gaggggatgg agggcgaagg
     1621 ccgagagggc gctgtagggc gaggtctggc tgcccgtgtc gttcaccggg aggagctgga
     1681 caaggcccat gccccaggag gaggccagat cgcccatcgc ggcgagatcg gggtactctc
     1741 cgcatcccgg ggacgaggcg ctgcgaaggc tcgagagcgg gacggcgagg ccgctcaggg
     1801 aatggtcgtc gtgggcgaag cgcatgtcgg gacctccgcc ctcaagtata gccgccaagg
     1861 cggccggagg aggaggagaa agcgtcggga cccgcgatgc ccctttaggc ctcgcgcccc
     1921 ggcgttcccg acccgaagac ggccttgagc gcctccttga cggtgcggac ccccttccat
     1981 tccgcagcct cggcgcctcg ctcgccgcgc ctcgcctcgg tctcgcccaa agggcccacg
     2041 agcctcgaaa agccgagggc gagggcggcc ttggccctgc gccgcatctg gcgcaccggc
     2101 ctcacctcgc ccgcgaggga gagctctccg gcgagggcga cctggagggg cagggggacg
     2161 cccgagcgag ccgagtacag ggcgcaggcc agggccagat cgatccccgg ctcggtgagc
     2221 cgtatccccc cggcgacgtt gacgtagagg tcctggtcgg agaacctcgc gcccgtgtgt
     2281 ttctcgagga cggccgcgat gcgcgacacc ctcgcagcgt cgacccgctc cgagtacacc
     2341 cgcgtgagcc cggccttcgc cggcacggtc agggcctgga tctccacgag gaggatgcgc
     2401 gagccctcgt ggacgggggc cacggccgcc cccgcgggca tgccctcgtc ccggtgcacg
     2461 aggaagaggc cggcggcgtc gctgacctcc tcgagccctg cctcgcccat ccggtacagg
     2521 ccgacctcct cggcgctccc gaagcggttc ttgctcgcgc ggaggacccg catgtccccc
     2581 tcgccctgct cgaagccgag cacggcgtcg accaggtgct cggccgcctt gggaccggcg
     2641 atgatcccct ccttggtgac gtgggcgacg aggatgatcg ccgagtcgtg ctccttcgcc
     2701 cagctcgtga gctccatcgc gcagtacttg atctggttcg ccgtgcccgg cacggccccc
     2761 gcctcctctg ccctgagggt ctggatcgag tcgaccacgg cgatggtcgg gcgcacggcg
     2821 tcgagggtgg ccctgacgag gccgaggtcg ccggagcaga agacctcgag cccgtcgcgg
     2881 atgcagccga gcctctcggc gcggagcttg atctgggccg gggactcctc gcccgagatg
     2941 tacaggagcc tgcccttggt cgcggccttg gcgaggatct ggaggaggag ggtgctcttg
     3001 ccgatgcccg gctcgccccc gaggaggacc accgagccgc gcatgatccc cccgccaagg
     3061 accctgtcca tctcccccga tccggtcggg atcctggtct cctcgcccgc ctcgatcgag
     3121 gctatgggga gggggaaggt ctccccgccc cgctctgccc tggcctgggc gagggctgtc
     3181 tcgcggaggg tgttccactg gccgcattcg gggcagcgcc ccagccactt cgactcgctg
     3241 tggccgcagg cgctgcagcg gtagacaagg ctcttcttct tggctgtcat aacgccccat
     3301 cgtacggggt atcgcctgac gcttcaagga tcggcgccgc cgcattgaag ccgggcccgg
     3361 acggactata cttgggccag ccacattcca aaggagactt cgcgatggca cagcgatccg
     3421 cgtccgactt cggctcgatc ttcttcctcc agctcgcgct tggtgccttc ttcctcgccc
     3481 tcggcatcat gggcgtcacc gactaccagc acagcaccag gggagtgatg agcctcttcg
     3541 cgaagaacag cgccctcaag ctcacgacgg cgatcatcga gctggccatg ggcggggtcc
     3601 tcctcctggg cctggtcttc tcggtgtcct cggccctcgc caggatcctc ggcatcgtcc
     3661 tcttccttct gtgggccgtg tacatcgtca tgaacttcat cgccaaggac atcttcgagc
     3721 ccagcttcgt cccctggctc tacaacatct cctggaattc ggtgatcctg gtcgcccttt
     3781 ggacggtcgg gaccaggtat agggcctagc aggccctttg gcccgcaaag ccggatcggg
     3841 cggggacgct gagggcgaag cgggtcccgc ccgtctctcg ccgctccagg aggacggtcg
     3901 cgttgagctg ggaggcgagg ctccgcacca gggtcaggcc gaggccgggg ccggcctcaa
     3961 gccccggcgg gatgcccacg ccgtcgtcga tgacctcgat cgtcgtgcgg ccctcctcgg
     4021 cccgggcatg cacccagatc gagccgccgc tgcggccgac aaaggcgtgg cggcaggcgt
     4081 tcgcgaccac ttcggcggtg atgatgccga tcgcgacggc gcattggatg tcgagcctga
     4141 tgtcgtgggc gtcgacctcg atcctccgcc tgcccaggcc cccctcggcc tgctggatct
     4201 cgccggcgaa gcgcgagagg aatgcgccga gcctcacccc gtccatcctc ggctcggtgt
     4261 agatctcctc gtggacgaag gccatggcgt cgacgcggcg cttcgtcgcc tcaagg
//
LOCUS       DASSQV010000455         4518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_853030, whole genome shotgun sequence.
ACCESSION   DASSQV010000455 DASSQV010000000
VERSION     DASSQV010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853030"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFL04_07990"
     CDS             <1..453
                     /locus_tag="VFL04_07990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7839685.1"
                     /translation="RHHSRAGIIILDVDHFKLFNDTWGHVAGDVILSEIAHTIKREVR
                     SEDVPARFGGEEFCILAIECEEQALFDMAERIRMAIEGLKVEFRSQSLSVTVSLGCCL
                     LDPASGHDPQTFVEMADRALYISKSQGRNRSTLYRHGLLGRATALKPR"
     gene            complement(518..1072)
                     /locus_tag="VFL04_07995"
     CDS             complement(518..1072)
                     /locus_tag="VFL04_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839686.1"
                     /translation="MSSRFTRYIGRLIRAALVLGVLFLGLYAAHAYYGFLDDQIAGVQ
                     AFMTELEGSLSVEALAGISMGSLVLVIALCLMPIFWRKIDTRSYVRGLWRGIVSAFVF
                     LASSALFSFAERASKVYLIAAILGVIIVSALLIEAVSLAVREEEERSLRTDIVASISS
                     GLLFGVLIQLGQVALAWAKGLVGS"
     gene            complement(1078..1659)
                     /locus_tag="VFL04_08000"
     CDS             complement(1078..1659)
                     /locus_tag="VFL04_08000"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7839687.1"
                     /translation="MKIRYNAPATLTFTLLAALVLLLSGTFLPGLTKEWFSTPAPFRA
                     NAAQDYVKLFTHVIGHASLDHFISNFSFILLLGPILEATYGSVMVLIMMAVTALVTGG
                     LQVFLFPNTILLGASGIVFMMILLASFTNFRKGEIPLTFILIMVLYLGREVWNAVASK
                     FVTGQPDNISQFAHVIGGLVGSFFGFLKSARRE"
     gene            complement(1707..2486)
                     /locus_tag="VFL04_08005"
     CDS             complement(1707..2486)
                     /locus_tag="VFL04_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET7839688.1"
                     /translation="MTRIFSWNVNGIRACAGKGLFEWLEAESPDILCLQETKAQPEQL
                     TKDFFSAGGGAYKTFWASAKKKGYSGTAIYCKKEPRSISFLGVSEYDDEGRTLVADFG
                     SFVLISAYFPNSQDGGARLDYKLEFCDTVLKRCDKLVKEGRHVVVTGDFNIAHKPIDL
                     ANPKANEGNPGYLPEERAWMDKYTSSGYVDTFRHFDKEPGRYTWWTVRVKTARENNIG
                     WRLDYFCVDQGLMAKVKASSIHPGVLGSDHCPVSLDLDLKI"
     gene            complement(2483..3703)
                     /locus_tag="VFL04_08010"
     CDS             complement(2483..3703)
                     /locus_tag="VFL04_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET7839689.1"
                     /translation="MSLDGGGPARAASIRERNERLVLRLIFRKGPLSQSEAAKLTGLK
                     PPTVFRIFSELERAGLIAADPSRRAEPSERKGRRPQDYRVVPSAAYAVGVDFWAGSAA
                     AVVQDFSSRLVASRLASFEDPPDAESAQAAIETIIREVLAEAGVGSEALLGIGVGAPG
                     SVSVDEGVVHFYSRIPGMRGYPLGARLKERFGVPVSVHNNASVVAMAEHRYGVASGVG
                     SLFAFLIRSGLGGAYLQGGKALVDRGRTAFEVGHLSVDMRGQPCSCGNSGCLETYLSE
                     DAFLAAASGFIGTAGGGEGIAALDRALAEGRPGLEEALAPSLAIASAAVRNIRRLLAP
                     EAVLFVTRSAALSSRLAVAASADEDCDDGRFGPPGAPVLAAAYDPLLACRAACDLVYD
                     AFFEGGISIAKEEP"
     gene            complement(3700..>4518)
                     /locus_tag="VFL04_08015"
     CDS             complement(3700..>4518)
                     /locus_tag="VFL04_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839690.1"
                     /translation="AKDSPFIQGMEIELKVPAAVMAVPGGFAYELWRRVDPAPEKSRF
                     GYRGERIITQMLPARAGLVIQVPIRKDHSLKSGPYATLVPVVVEAKDFPFLFKLLAVS
                     KGFSSEVENAQFQVRVRPILTDEGALSIRLRYPEGQAEKSPVAITVDDRKVEASGPLL
                     LKAGVHRLHIASDNYREENRSFTIEQGKALELVVELQDTTPVLLIEAPDSAVVLLDGQ
                     RLDHVAKPQIQVEAGEHSATCRIGDYTITRKFTAYRGKTYHLVLAIDLQVQESP"
BASE COUNT          741 a         1412 c         1672 g          693 t
ORIGIN      
        1 cgccaccact cgagggcggg gatcatcatc ctcgacgtcg accacttcaa gctcttcaac
       61 gacacctggg gccacgtggc gggggacgtc atcctctcgg agatcgccca cacgatcaag
      121 cgcgaggtca ggtccgagga cgtgcccgcc cgcttcgggg gcgaggagtt ctgcatcctg
      181 gcgatcgagt gcgaggagca ggccctcttc gacatggcgg agcggatccg catggccatc
      241 gagggcctta aggtcgagtt caggtcccag agcctctcgg tgacggtgag cctgggctgc
      301 tgtctcctcg accccgcgag cggccacgac ccccagacct tcgtcgagat ggccgaccgc
      361 gccctctaca tctccaagtc ccagggccgg aaccgcagca ccctgtaccg ccacggcctc
      421 ctcggccggg cgaccgcgct caagccgaga taggccaccg cccctggcag gcccgccaag
      481 ccaggggccg ccgggaggac cgcaatcctt ggtcggctca gctgccgacg aggcccttcg
      541 cccacgcgag ggccacctgg cccagctgta tgaggacccc gaagaggagg cccgaggaga
      601 tcgaggccac gatgtcggtg cgcagggagc gctcctcctc ctcgcgcacc gcgagcgaga
      661 cggcctcgat gaggagggcg ctcacgatga tgaccccgag gatcgccgcg atcaggtaga
      721 ccttgctcgc gcgctcggcg aaggagaaga gcgcgctcga ggcgaggaag acgaaggccg
      781 agacgatgcc cctccagagc ccccgcacgt agctccgcgt gtcgatcttc ctccagaaga
      841 tgggcatgag gcagagggcg atcacgagga cgagggagcc catggagatg ccggcgaggg
      901 cctccacgga caggctgccc tcgagctcgg tcatgaaggc ctgcacgccc gcgatctggt
      961 cgtccaggaa gccgtagtag gcgtgggcgg cgtagaggcc gaggaaaagg acgcccagga
     1021 ccagggcggc gcggatgagc ctgccgatgt agcgggtgaa tctggagctc acgttgtcta
     1081 ctcccttctc gccgacttga ggaagccgaa gaagctgccg acgagaccgc ctatcacgtg
     1141 ggcgaactgc gagatgttgt cgggctgtcc ggtgacgaac ttgctggcca ccgcgttcca
     1201 gacctcgcgc ccgaggtaga ggaccatgat gaggatgaag gtcaggggga tctcgccctt
     1261 gcggaagttc gtgaaggagg cgaggaggat catcatgaag acgatgcccg aggccccgag
     1321 gaggatggtg ttggggaaga ggaagacctg aaggcccccc gtgaccaggg cggtgactgc
     1381 catcatgatc aggaccatga ccgagccgta ggtggcctcg aggatggggc ccagaaggag
     1441 gatgaagctg aagttcgaga tgaagtggtc gaggctcgcg tggccgatga cgtgggtgaa
     1501 gagcttcacg tagtcctggg ccgcgttcgc gcggaagggc gcgggcgtgg agaaccattc
     1561 cttggtaagg ccggggagga aggtgcccga caggaggagg accagggccg ccagaagggt
     1621 gaaggtgagg gtagccggcg cgttgtatcg tatcttcatc gagcagcctc catggtttca
     1681 tcgccgccct tgggcgggcg cttgggtcag atcttcaggt cgaggtcgag ggacacgggg
     1741 cagtggtcgg agccgaggac ccctggatgg atggacgagg ccttgacctt ggccatgagg
     1801 ccctggtcga cgcagaagta gtcgaggcgc cagccgatgt tgttctcgcg cgcagtcttc
     1861 acgcggaccg tccaccaggt gtagcgtccg ggctccttgt cgaagtgccg gaaggtgtcg
     1921 acatagcccg aggaggtgta cttgtccatc caggcccgct cctcggggag gtagccggga
     1981 ttgccctcgt tggccttggg gttggcgagg tcgatgggct tgtgggcgat gttgaagtcg
     2041 ccggtcacca ccacatgcct cccctccttc accagtttgt cgcagcgctt caggaccgtg
     2101 tcgcagaact ccagcttgta gtcgaggcgg gccccgccgt cctggctgtt cgggaaatag
     2161 gccgagatga ggacgaagga gccgaagtcg gcgaccaggg tcctgccctc gtcgtcgtac
     2221 tcgctcaccc cgaggaagga gatcgagcgg ggctccttct tgcagtagat cgccgtgccc
     2281 gagtagccct tcttcttggc cgaggcccag aaggtcttgt aggccccgcc cccggccgag
     2341 aagaagtcct tcgtcagctg ctcgggctgg gccttggtct cctggaggca gaggatgtcg
     2401 ggcgactcgg cctcgagcca ctcgaacagg cccttgccgg cgcaggcgcg gattccgttg
     2461 acgttccacg agaatatgcg ggtcacggtt cctcctttgc gatgctgatg cctccctcga
     2521 agaaggcgtc atagacgagg tcgcaggccg ccctgcaggc gagcaggggg tcgtaggccg
     2581 cggccagcac gggggccccg ggggggccga agcgcccgtc gtcgcagtcc tcgtcggccg
     2641 aggcggccac ggcgaggcgg ctcgagaggg ccgcggagcg ggtcacgaaa agcacggcct
     2701 cgggggcgag gaggcgcctt atgttcctca ccgctgccga ggcgatggcg aggctggggg
     2761 cgagggcctc ctcgaggccg ggcctgccct cggcgagggc ccggtcgagg gcggctatgc
     2821 cctcgccgcc gcccgccgtc ccgatgaagc ccgaggcggc ggccaggaag gcgtcctctg
     2881 agaggtaggt ctcgagacag cccgagttcc cgcaggagca gggctggccg cgcatgtcca
     2941 cggagaggtg gccgacctcg aaggcggtcc tgccgcggtc gacgagggcc ttgccgccct
     3001 ggaggtaggc cccgccgagg cccgagcgga tgaggaaggc gaagagcgag ccgacgcccg
     3061 aggcgacccc gtagcggtgc tcggccatcg cgacgaccga ggcgttgttg tggacgctca
     3121 ccggcacgcc gaagcgctcc ttgagccgcg cgccgagggg atagccccgc atgccgggga
     3181 tgcgggagta gaaatggacg accccctcgt cgacgctcac cgagcccggg gcgcccaccc
     3241 cgatgccgag gagggcctcg ctgccgaccc cggcctcggc gaggacctcg cggatgatcg
     3301 tctcgatggc ggcctgggcg ctctcggcgt cgggggggtc ctcgaaggag gcgaggcgcg
     3361 aggcgacaag gcgtgaggag aagtcctgga ccacggccgc cgccgagccc gcccagaagt
     3421 cgacgccgac ggcgtaggcg gcggagggga cgacgcggta gtcctggggc ctgcggccct
     3481 tgcgctcgct gggctcggcg cggcggctcg ggtcggccgc gatgaggccg gccctctcga
     3541 gctcggagaa gatgcggaac acggttgggg gcttgaggcc ggtgagcttg gccgcctcgc
     3601 tctgcgagag ggggcccttg cggaagatga gcctgaggac caggcgctcg ttccgctcgc
     3661 ggatggacgc cgccctggcg gggccccctc cgtcgaggct catggactct cctgcacctg
     3721 gaggtcgatc gcaaggacga ggtggtaggt cttgccgcgg taggctgtga acttcctcgt
     3781 gatcgtatag tccccgatcc ggcaggtggc gctgtgctcc cccgcctcga cctggatctg
     3841 aggcttcgcc acgtggtcga gcctctggcc gtcgaggagg acgacggccg agtcgggggc
     3901 ctcgatgagg aggacggggg tcgtgtcctg gagctcgacc acgagctcga gggccttgcc
     3961 ctgctcgatc gtgaaggagc ggttctcctc gcggtagttg tccgaggcga tgtggaggcg
     4021 gtggaccccg gccttgagga ggagggggcc ggaggcctcg accttcctgt cgtccacggt
     4081 gatggccaca gggctcttct cggcctggcc ctcggggtag cggagcctga tgctcagcgc
     4141 cccctcgtcg gtgaggatcg gcctgaccct cacctggaac tgggcattct ccacctcgct
     4201 ggagaagccc ttggatacgg ccaggagctt gaagaggaag gggaagtcct tggcctcgac
     4261 caccacgggc acgagggtcg cgtagggccc gctcttgagg ctatggtcct tgcggatcgg
     4321 cacctggatc acgaggccgg cccgggcggg cagcatctgg gtgatgatgc gctcgccgcg
     4381 atagccgaac ctggacttct cgggggcggg gtcgaccctg cgccagagct cgtaggcgaa
     4441 gcccccgggc acggccatga cggccgcggg caccttgagc tcgatctcca tgccctggat
     4501 gaagggggag tccttggc
//
LOCUS       DASSQV010000456         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2097207, whole genome shotgun sequence.
ACCESSION   DASSQV010000456 DASSQV010000000
VERSION     DASSQV010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097207"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFL04_08020"
     CDS             <1..218
                     /locus_tag="VFL04_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7839691.1"
                     /translation="RGGGRGLGFQLGPNYPQGGCGHTGFTGGYLHLNPPTGLVVAILA
                     NRLHVPSPGDLNPFRRELSEAVIAAFG"
     gene            271..1890
                     /locus_tag="VFL04_08025"
     CDS             271..1890
                     /locus_tag="VFL04_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HET7839692.1"
                     /translation="MHWTQEYLPLGSMGPSALAAAAPLLLLFVLLALGKVKGWVAALA
                     STLAAGLVATLVWRMPLAAALSSGLLGSAFALFPILWILFAALWIYHLCVGSGQFDLI
                     RRMVAGLTGDRRLQALFIAFAFGAFLEGTAGFGVPVAITSAMLVGLGFPPELAATLCL
                     LANSSPVAFAAAGVPIATAAQVSGLDPIAIGRAVGLQLPLLSMSVPLWLCVLLCGWRR
                     SAEILPAILVAGFSLAGSQFLISSTLGPWTTGVLSGLAAMGSLALLLRFWKPRQIWDF
                     GGATGGQVGARPGEGMPFSAALRAWSPYLLTSAFVLFWSTPWAKALFRPASLLVHWPA
                     LDGLAMRTSPVVHPDTPYAAVLDLSILASPGTAIFLAGILSMLILPGLGLRGALAALG
                     RTLRDLRFTIPTVCFVLATAYLMNYSGMSSTIGLALSGTGALFPLFAPLLGWIGVLLT
                     GSDTSSNALFCSLQATTARQLGLDPVLVVASNTSGGVAGKMISPQSLSVATAATGLTG
                     REGQLFRSTFLHSLAMALVVGLVTLAMVYLF"
     gene            1977..>2518
                     /locus_tag="VFL04_08030"
     CDS             1977..>2518
                     /locus_tag="VFL04_08030"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7839693.1"
                     /translation="MDERYIVDNAAFLPTPALLFYEEAIRANHELAIRIAGSASRLRP
                     HIKTHKTPEIVKIALGFGISKFKCATIAEAEMLAAAGAKDILVAYQLVGRNAERLMAL
                     ARRHGDIRFSTLVDAAEPLARLEGLAAQAMVGLGVFLDLDVGQHRTGIAPGRDAEEIY
                     AIFARSGRLRPAGLHCYDGQ"
BASE COUNT          331 a          979 c          758 g          450 t
ORIGIN      
        1 aaaggggagg ggggaggggc ctcggtttcc agctcgggcc gaactatccg caggggggat
       61 gcggccacac gggcttcacc ggcggctatc tgcatctgaa cccgcccacc gggctcgtgg
      121 tcgcgatcct cgcgaaccgc ctccatgtcc cctcgcccgg cgacctcaac ccttttagac
      181 gcgagctctc agaggccgtg atcgctgcct tcggctgaag ctgagccggg ccttgcgccg
      241 gggtggcatg agggccgata gtgtcggatc atgcactgga cccaggaata cctgcccctc
      301 ggatcgatgg ggccatcggc cctggccgcg gccgcacccc tcctcctcct cttcgtgctc
      361 cttgccttgg gcaaggtcaa aggatgggtc gccgccctcg cctcgaccct ggccgccggc
      421 ctcgtcgcca ccctcgtctg gcgcatgccc ctcgccgccg ccctctcctc gggcctcctt
      481 ggctcggcct tcgccctctt ccccatcctc tggatcctat tcgccgccct ctggatctac
      541 cacctctgcg tgggttcggg ccagttcgac ctcataagga ggatggtcgc tgggcttacc
      601 ggggacaggc ggcttcaggc cctcttcatc gccttcgcct tcggcgcctt cctcgagggc
      661 acggccggtt tcggagtccc cgtcgccatc acctcggcga tgctcgtggg cctgggcttc
      721 ccgcccgagc tcgccgcgac cctctgcctc ctcgccaact cctcccccgt ggctttcgcg
      781 gctgccggag tgcccatcgc cacggccgcc caggtctcgg gcctcgaccc aatcgccatc
      841 gggcgggcgg tcggcctcca gctgcccctc ctgtccatgt cggtgccgct gtggctctgc
      901 gtactcctct gcggctggag gcggagtgcc gagatcctgc ccgccatcct cgtggcgggc
      961 ttcagcctcg ccggctcgca gttcctcatc tcgagcaccc tcgggccctg gaccacgggg
     1021 gtcctttcgg gcctcgcggc aatgggcagc ctcgccctac tcctgcgctt ctggaaaccg
     1081 aggcagatct gggacttcgg cggcgccacg ggagggcagg tcggagcaag accgggcgag
     1141 ggcatgccct tcagtgccgc cctccgcgcc tggtcgccct acctcctcac ctcggccttc
     1201 gtcctcttct ggagcactcc ctgggcgaag gcgctcttca ggcctgcaag cctcctcgtc
     1261 cactggccag ccctcgacgg tcttgcgatg aggaccagcc ccgtcgtcca tcccgacacc
     1321 ccgtacgccg ccgtcctcga cctgtcgatc ctggcctcgc ccggcacggc gatcttcttg
     1381 gcgggcattc tgtcaatgct catcctgcct ggcctcggcc tccggggcgc cctcgccgcc
     1441 ctgggacgga ccctgcgtga cctgcgcttc accataccga ccgtctgctt cgtcctcgcc
     1501 acggcctatc tcatgaacta ctccggcatg agctcgacga tcggcctcgc cctttcgggg
     1561 acaggcgccc tcttccccct cttcgcgccc ctgctcggct ggatcggcgt cctcctcacc
     1621 ggctcggaca cctcctcgaa cgccctgttc tgctcccttc aggcgacgac ggcccgacag
     1681 ctcggcctcg atcccgtcct cgtggtcgcc tccaacacct cgggaggggt ggccggcaag
     1741 atgatctcgc cccaaagcct gtcggtggcc acggccgcta caggcctcac cggcagggag
     1801 gggcagctct tccgctccac cttcctgcac agcctcgcga tggccctcgt cgtgggcctc
     1861 gtcaccctgg cgatggtcta cctgttctga gccgcccaga ggccgccgcc ccgagcggcc
     1921 gaggccgctg ggggacagcc gtccggggac agtcacgaaa taaaaaggag aagaccatgg
     1981 atgagcgcta catcgtcgac aacgccgcgt tcctgccgac gcccgccctc ctgttctacg
     2041 aggaggcgat cagggccaac cacgagctcg cgatccgcat cgccggctcg gcctcccgac
     2101 ttcgccccca catcaagacc cacaagacgc ccgagatagt gaagatcgcc ctaggttttg
     2161 gcatctcgaa gttcaagtgc gccaccatcg ccgaggcgga aatgctggca gccgccggag
     2221 cgaaggacat cctcgtcgcc tatcagctcg tgggcaggaa cgccgagcgg ctcatggccc
     2281 tggctaggcg gcacggcgat atcaggttct ccaccctcgt cgacgccgcc gagcccctgg
     2341 cccggctcga gggcctcgcg gcccaggcga tggtcggcct gggcgtcttc ctcgacctgg
     2401 acgtgggcca gcaccggacc ggcatagccc cgggccggga tgccgaggag atctacgcca
     2461 ttttcgcccg cagcgggcgg ctcaggcccg ccggcctcca ttgctacgac ggccagaa
//
LOCUS       DASSQV010000457         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1224830, whole genome shotgun sequence.
ACCESSION   DASSQV010000457 DASSQV010000000
VERSION     DASSQV010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224830"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFL04_08035"
     CDS             complement(<1..478)
                     /locus_tag="VFL04_08035"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HET7839694.1"
                     /translation="MPRPRQTAAAGLAFVFLVAAGLGLEAQNFDNPLILQRADPYILL
                     HSDGYYYFCATVPEYDRIELRRARDIAGLREAKPVVVWRKQRSLLGEDRNLWAPELHR
                     IDGKWYIYYAAGTSAAPYDVRIRVIENASSDPLSGTWTDLGRLDTGRNSLCLDATSF"
     gene            complement(575..1900)
                     /locus_tag="VFL04_08040"
     CDS             complement(575..1900)
                     /locus_tag="VFL04_08040"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7839695.1"
                     /translation="MAVAKLGGMRGFFVLWLGQAFSILGGSMALFAITLWIYQRTGQA
                     TPVAMLGFFNLMPTLLFGLFTGVLVDRHDRRLMMLLSDLATLAINLFLIGFAVSGSLA
                     VWQLYAAAFVVGTFQTFQWPATSAAVTLMVDKKHYARAASLLEMAGMSSGVVAPLLAG
                     SLLGFLGLGGVLAVTATGSALSVASLFLIDLPARAPHEGPRPRILSEVRDGLAYLLER
                     PPLIGLQSTFLLGNFFFTLSYALLGPMILAKTGNNAISFASVETAGAVGGLVGSGLIA
                     LLGGTKNRVLGILLGWVGSGLGGLLIIGLGRGLQLWMLGSFLGAALGSLNYASNQALW
                     QSKVAPDLQGRVFAVRRLIATGVIPLASLAAGPLADRLLEPAMREGGSLVGLLGPVFG
                     VGPGSGIAIIFACCGIATALVGSLAWFVPVIRRAESLIPDHDAPRIQSP"
BASE COUNT          351 a          636 c          800 g          269 t
ORIGIN      
        1 cgaagctggt cgcgtcgagg cagaggctgt tccttccggt atcgaggcgc ccgaggtcgg
       61 tccaggtccc cgagaggggg tcgctcgagg cgttctcgat caccctgatg cggacgtcgt
      121 agggcgcggc gctggtgccg gcggcgtagt agatgtacca cttcccgtcg attctgtgca
      181 gctcgggggc ccagaggttg cggtcctcgc cgaggaggct cctctgcttc ctccagacca
      241 ccaccggctt ggcctcgcgg aggccagcta tgtccctggc ccgccgcagc tcgatcctgt
      301 catactcggg gacggtcgcg cagaagtaat agtagccgtc gctgtgcagg aggatgtagg
      361 gatcggcccg ctggaggatc aggggattgt cgaagttctg ggcctcaagg ccgaggccgg
      421 cggcgacgag gaagacgaag gcgaggccgg cggcggcggt ttggcggggt ctgggcatgg
      481 tttccctcca ctgcggccat tgtggcccca gacagggctc gggtatttgg acgcatcgtc
      541 ctttttgtgc accatggcgc cggcccaccg ggcctcaggg cgactggatc ctcggtgcgt
      601 cgtggtcggg gatgagggat tcggccctgc gtatcacggg cacgaaccag gccaggctcc
      661 ccacgagggc ggtggcgatc ccgcagcagg cgaagatgat ggcgatgccc gagcccggtc
      721 cgacgccgaa gacgggtccc aggaggccga cgaggctccc tccctcgcgc atcgcgggct
      781 cgaggaggcg gtcggccagg gggccggcgg ccaggctcgc cagagggatt acccccgtgg
      841 caatgaggcg gcgcacggcg aagaccctgc cctgcaggtc aggcgccacc ttggactgcc
      901 agagcgcctg gttcgaggcg tagttgaggc tgcccagggc cgcgcccagg aaggagccga
      961 gcatccagag ctggaggcca cggcccaggc cgatgatgag gaggccaccg aggcccgagc
     1021 ccacccagcc gaggaggatc ccgagcaccc ggttcttcgt ccccccgagg agggcgatga
     1081 ggccggagcc aacaaggccg ccgacggcgc ccgccgtctc caccgaggcg aaggagatgg
     1141 cgttgttgcc cgtcttcgcc aggatcatgg ggccgaggag ggcatagctc agggtgaaga
     1201 agaagttgcc gaggaggaag gtgctctgga gcccgatgag gggcggacgc tcgaggaggt
     1261 aggccaggcc gtcgcggacc tcgctgagga ttcggggacg gggtccctcg tggggggccc
     1321 tggccggaag gtcgatgagg aagaggctcg ccaccgagag ggcgctcccc gtcgccgtca
     1381 ccgcaaggac tcccccgagg ccgaggaagc ccaggaggga gcccgcgagc aggggggcga
     1441 cgacccccga ggacatgccc gccatctcga ggagggaggc ggcgcgcgcg tagtgcttct
     1501 tgtccaccat gagggtgacg gccgccgagg tcgcgggcca ctggaaggtc tggaaggtcc
     1561 cgaccacgaa ggccgcggcg taaagctgcc agacggcgag ggagcctgag accgcaaagc
     1621 cgatgaggaa gaggttgatc gcgagggtcg cgaggtcgct caggagcatc atgaggcggc
     1681 ggtcgtggcg gtcgacgagg acccctgtga agaggccgaa gagcagggtc ggcatgaggt
     1741 tgaagaagcc gagcatcgcc acaggcgtcg cctggccagt cctctggtag atccagaggg
     1801 tgatggcgaa gagggccatg gagccgccga ggatggagaa ggcctgtccg agccacagta
     1861 caaagaagcc ccgcatgccc ccgagctttg cgaccgccac gcccatcacc tccggccgaa
     1921 cacaatacgg agggaggggc cgcctgccaa ggggccgggg cgccgggtgt ccggggcgcc
     1981 gggtcggcgc gcggcgccct gcgggtcaaa gggccggacg gggggccggc ccgaaggccg
     2041 gcccggcgcg gaagcc
//
LOCUS       DASSQV010000458         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_728382, whole genome shotgun sequence.
ACCESSION   DASSQV010000458 DASSQV010000000
VERSION     DASSQV010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728382"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..920)
                     /locus_tag="VFL04_08045"
     CDS             complement(78..920)
                     /locus_tag="VFL04_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7839696.1"
                     /translation="MMILLRLLFAIMVASFASFGASFLSSAWRRRRKADAAAMAVEGL
                     LPGFDCGLCGAEDCRSYSIDIATKGADPGLCSPGGMRTESRIRSYLGESRGDGRAVRR
                     LASILCAGDDKVAARDFEYDGQDDCLAAAALYGGPKRCKDGCLGFGTCARVCPLGAIR
                     MEDGLARVDPGLCTGCGLCVPVCPNSIIVLRPESERWRVACSSHREPEAKAADCRKAC
                     IACGECVKNSFQGEFRLEKNLASTRPVENGQWELIAPVCPTGAIADRDMRKKTRSSLR
                     NQEH"
     gene            complement(917..1501)
                     /locus_tag="VFL04_08050"
     CDS             complement(917..1501)
                     /locus_tag="VFL04_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HET7839697.1"
                     /translation="MSYIGLVLSAVFAANSMLVYGFGRLPRFRAVGAGWLPPLLALVL
                     VNALASTIFWAIRNLVLVPLGLASIDLFVFVLLAVPLIKSLTHASTLGEGFLARVGIV
                     ADELVVGTLSFGVALIVSRGGYRIVEALIASAASGLGYWLAAVVLERIEARLELSSVP
                     DPFRGAPVMIVSTGLIAMALMGIDASFVRNLVGR"
     gene            complement(1498..2124)
                     /locus_tag="VFL04_08055"
     CDS             complement(1498..2124)
                     /locus_tag="VFL04_08055"
                     /inference="COORDINATES: protein motif:HMM:NF014557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HET7839698.1"
                     /translation="MKRGTDSFVPNPVLGGLVGVFPLAVAANSLMNGLVLGIGSAFCS
                     AMLASLLPALRAVAPERLRAPLSLGIAAALALLYTDIVLALAPVVAAGLGVYLPLLAV
                     NCMSLHILRSGPAAQGRYGSAASRMGIVFREAFGYLLVALLVGALRELAGSGSLVLPA
                     VLSVGAGIQLTAEPPLRFLVSPAGGFILIGCGVALYRVLLRGAGRRIP"
BASE COUNT          446 a          678 c          709 g          314 t
ORIGIN      
        1 tggttatatg cgccaatgat gatttggctt ttttgcattg gccgacgatc cttccacttt
       61 atgcgggcga ttatagctca gtgctcttgg ttccgtaagg acgatcgggt ctttttccgc
      121 atatcgcgat ccgctattgc cccagtcggg cagacgggag cgatgagttc ccattgaccg
      181 ttttcgaccg gcctcgtcga ggccaggttc ttctccagcc tgaactcacc ctggaaggag
      241 ttcttgacgc actcgccgca agcgatgcag gccttcctgc agtcagcggc ctttgcttcg
      301 ggctcgcggt gcgaggagca ggccaccctc cagcgctcgc tctcgggcct gagcacgatg
      361 atcgagttgg ggcagacagg cacgcagaga ccgcagccag tacagaggcc gggatcgacg
      421 cgcgcgagcc catcttccat ccggatggcg ccgagcgggc aaaccctcgc gcaggtccca
      481 aagccgaggc aaccatcctt gcagcgtttg gggccgccat agagggcggc ggcggcgagg
      541 cagtcatcct gcccgtcgta ttcgaaatcc ctggccgcga ccttgtcgtc ccccgcgcag
      601 agtatggacg cgagccttct gaccgcccgt ccatctccgc ggctctcgcc gaggtaggat
      661 cggatacggg actcggtccg cattccccca ggtgagcaga gacctggatc ggcccctttg
      721 gtggcgatgt cgatcgagta ggagcggcaa tcctcggctc cgcagagtcc gcagtcgaat
      781 ccaggcagga ggccttccac ggccatggca gctgcatcgg ccttccttcg cctccgccag
      841 gccgatgaca ggaaggatgc gccgaaagac gcgaacgacg cgaccatgat cgcgaaaagg
      901 agcctcagga ggatcatcat cgtcccacca agttcctgac gaaggacgcg tcgatgccca
      961 tcagggccat ggctatgaga ccagtggata cgatcatcac gggggcgcca cggaaggggt
     1021 caggcacgga gctgagctcg agcctggcct cgatgcgttc aaggacgaca gcggcaagcc
     1081 agtagccgag gccggaggcc gcgctcgcga tcagggcctc gacgatcctg tagccccccc
     1141 ttgagacgat gagggcgacg ccgaaactca gggtgcccac gaccagctca tcggcgacga
     1201 tgcctacccg ggcgagaaaa ccctccccca aggtggaagc atgggtgagg ctctttatta
     1261 gggggaccgc caggaggaca aagacgaaaa ggtcgatcga ggcgaggccg agaggcacga
     1321 ggacaagatt cctgatcgcc cagaagatgg tggatgcgag agcgttgacg agcacgaggg
     1381 ccagcaaagg gggaagccaa ccggcaccca ctgccctgaa cctcggaagg cggccaaaac
     1441 cgtagaccaa catggagttt gcggcgaaca ccgcggaaag caccagcccg atgtagctca
     1501 tggtatcctc cggcccgcgc ctcgaaggag gaccctgtac agagccacac cgcagccgat
     1561 caagatgaat ccacccgcgg gtgaaacgag gaaacgaagg ggaggctctg ccgtcagctg
     1621 gatccccgcg cccacagaga ggaccgccgg caggaccagg ctgcccgagc cggcgagctc
     1681 gcggagggcg cccaccagga gcgcgacaag cagatagcca aaggcctcac ggaacacgat
     1741 ccccatcctg gacgcggcgg aaccgtatcg cccctgcgcg gcagggccgc tccgaaggat
     1801 gtggaggctc atgcagttca ctgcaagcag gggcaggtag acgccgagac ccgcggcgac
     1861 gaccggggcg agggcaagga cgatgtcagt gtacagcagg gccaaggccg ccgcgatgcc
     1921 gagggaaagc ggggcacgca gacgctcggg ggcgacggcc ctcaaggccg gcaggaggct
     1981 ggcgagcatc gccgaacaga aggccgagcc gatgccgagc acgagtccgt tcatcaggct
     2041 gttggccgcc acggcgaggg ggaagacgcc cacgaggcca ccgaggaccg ggttgggcac
     2101 gaaggagtcc gttcctcttt tcatccgcgc tccttcgcca tgtcccg
//
LOCUS       DASSQV010000459         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_356257, whole genome shotgun sequence.
ACCESSION   DASSQV010000459 DASSQV010000000
VERSION     DASSQV010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356257"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VFL04_08060"
     CDS             <1..964
                     /locus_tag="VFL04_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET7839699.1"
                     /translation="ELGLYPEDGRLRGGRDPDAALQRRRTARDGLMARYRLILDDTGA
                     SLHYRALAAWRLGAEPQLLPPDLPDPQAPPERGQLEAFIAGFIGFGMADTAYSEAQDR
                     MAGLDQESVRRLVRDIAAAGHIDSSMRLIIALMARPDWEPRLSDYELLYPRPFISELR
                     SIRPRPDVSEDLIYGLVRSESYFSHDAYSRAGAIGLTQLMPGTAAEVASALGMAAYDL
                     RSPKDNIRIGAAMFADLLDETGGRAFRAMMAYNAGRSRLKKWMAESGDLPDDLMIEAL
                     GIEETRQYCRNILQATVMYGEIYYGIPVATSVGEIVEGQADKRP"
     gene            complement(996..1385)
                     /locus_tag="VFL04_08065"
     CDS             complement(996..1385)
                     /locus_tag="VFL04_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839700.1"
                     /translation="MVEAVSTNFLPISYVYASRYTSGRVSLPVLPREALYANFQHVGG
                     VPALDGTPAYSIDKLHILDMLIAGLESVKDQPLAAREAPATLNPGRVDALIAQYGAEY
                     HSLAAAPAQIYSPQPPSQPGILFSLAA"
     gene            complement(1474..2472)
                     /locus_tag="VFL04_08070"
     CDS             complement(1474..2472)
                     /locus_tag="VFL04_08070"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET7839701.1"
                     /translation="MEILDPGEENLRRAGALIAAGGLVALPTETVYGLGADAFNARAV
                     ARVFEAKARPSFDPLIVHIAALGDIDRVARRPSALARSLMEALWPGPLTLVLPKREEL
                     PDLVTSGLPTVAVRLPAHPVARAIIASSGTSVAAPSANPFGYISPTRAEHVARMLGDR
                     VDLIIDGGPCQVGVESTVLDMTVEPPRLLRPGGMELSRIEAVIGEISTKPLSPATEQD
                     RPAGPGLLDSHYAPRSPLVLLSREELRAMRPEGSARVGLLLFEDSSLEALGGREAFAV
                     TRLLSPSGDLVEAAASLFAHMHELDALGLDEIWAERVPEGGLGAAINDRLHKASRK"
     gene            complement(2479..>2973)
                     /gene="purE"
                     /locus_tag="VFL04_08075"
     CDS             complement(2479..>2973)
                     /gene="purE"
                     /locus_tag="VFL04_08075"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET7839702.1"
                     /translation="SDLPVMAETSRVLAELGVPCETTIVSAHRTPSRLVEYARSAAYR
                     GLEVIVAGAGGSAHLPGMTASMTVLPVIGVPIRTKTLDGLDSLLSIVQMPPGIPVATV
                     AVDAGRNAGLLAARILATSRPELRARVEAYAAALEAEVLAKAERLEGLGPEGYAGGPG
                     GAGA"
BASE COUNT          464 a          987 c         1091 g          431 t
ORIGIN      
        1 ggagctcggc ctctacccgg aggacggccg ccttcggggg ggccgcgatc ccgatgcggc
       61 gctccaacgt cgcaggacgg cgagggacgg cctcatggcc aggtacagac tcatcctgga
      121 cgacaccggc gcctcgctgc actaccgggc ccttgccgca tggaggctcg gagccgagcc
      181 ccagctcctc ccccctgatc tccccgatcc ccaggccccg cctgagcgcg gccagcttga
      241 ggccttcatc gccggcttca tcggtttcgg catggccgac accgcataca gcgaggccca
      301 ggacaggatg gccggcctcg accaggaatc ggtgaggagg ctggtccgcg acatagccgc
      361 ggccgggcat atcgactcct cgatgcggct catcatcgcc ctgatggcga ggccggactg
      421 ggaaccccgg ctttccgact acgagctgct gtatccccgc cccttcatat cggagctcag
      481 gtccatccgc ccccgccccg acgtgtccga ggacctcatc tatggcctcg tgaggtcgga
      541 gagctatttc agccatgacg cctactcgag ggcgggggcc atcggtctca cccagctcat
      601 gccgggcacg gccgccgagg tggcctcggc cctcggcatg gccgcctacg acctgcgctc
      661 acccaaggac aacatcagga tcggcgccgc gatgttcgcc gacctcctcg acgagaccgg
      721 cggcagggcc ttccgcgcga tgatggccta caacgccggc aggagcaggc tcaagaaatg
      781 gatggccgaa tccggggacc tgcccgatga cctcatgatc gaggccttgg gcatcgagga
      841 gacccggcag tactgccgca acatcctcca ggccacggtc atgtacgggg agatctacta
      901 cggcatcccc gtggcgacga gcgtcgggga gatcgtcgag gggcaggccg acaagcgccc
      961 ctgaggagtc tcgcggggat ccgggctgga gccggtcagg ctgccaggga gaagaggatc
     1021 ccgggctggg acggaggctg ggggctgtag atctgggcgg gggcggcggc caggctgtgg
     1081 tattcagccc cgtactgggc gatgagggca tcgaccctgc cggggttaag cgtggccggg
     1141 gcctcccgcg ccgcgagggg ctggtccttc acgctctcga ggccggcgat gagcatgtcg
     1201 aggatgtgga gcttgtcgat ggagtaggcc ggggtgccgt ccagggcagg tacgccgccg
     1261 acatgctgga agttggcgta gagggcctcc ctcggcagga cgggcaggga gaccctgccg
     1321 ctggtgtacc tcgaggcgta cacgtaggag atcggcagga aattggtgga aaccgcttcg
     1381 accatggctc catcctccga taaacctatc ggccggaggg gacccggagt ttacgaatat
     1441 taattcacgc cgatccaggc gccggagggc cggctatttc cgagaagcct tatggagcct
     1501 gtcgttgatg gccgcgccca agcccccctc tgggacgcgc tcggcccaga tctcgtcgag
     1561 gccgagggcg tcgagctcgt gcatgtgggc gaagagcgag gcggcggcct ccacgaggtc
     1621 ccccgatggg gagaggagac gggtcaccgc aaatgcttcc cgacctccca gggcctcgag
     1681 cgaggagtcc tcgaaaagca ggagtccgac ccgcgccgat ccctccggac gcatggccct
     1741 cagctcctcc ctcgacagca gaacgagggg gctgcggggg gcatagtggc tgtcgaggag
     1801 accggggccc gccggcctgt cttgctccgt ggccgggctg agcggcttgg tcgagatctc
     1861 gccgatgact gcctcgatcc gggacagctc catcccgccc ggcctgagca gacgcggggg
     1921 ctcgaccgtc atgtcgagga cggtcgactc gacgcccacc tggcagggcc ctccgtcgat
     1981 gatgaggtcg acccgatcgc cgagcatgcg ggccacgtgc tcggccctgg tcggcgagat
     2041 gtagccgaag gggttggcgc tcggcgccgc gaccgatgtg cccgagctgg cgatgatggc
     2101 cctggccacg gggtgggcgg ggagacggac ggctacggtg ggcaggccgc tcgtgacgag
     2161 gtcgggcaac tcctcgcgct tcggcaggac gagggtgagg gggccgggcc agagggcctc
     2221 catgagggag cgggcgaggg ccgagggcct tctggcaacg cggtcgatgt cgcccagggc
     2281 ggcgatgtgc acgatgaggg ggtcgaagct cggcctggcc ttggcctcga agaccctcgc
     2341 gacggcgcgg gcgttgaagg cgtcggcccc gaggccgtag acggtctcgg tggggagggc
     2401 gacgaggccc cctgccgcga tcagggctcc cgcccgcctg aggttctctt cacccggatc
     2461 aaggatctcc acgccggtct aggcccccgc cccgccgggg cccccggcgt agccctcggg
     2521 gccgaggccc tcgagccgct cagccttggc gaggacctcg gcctcgaggg cggcggcgta
     2581 ggcctcgacc ctcgccctga gctcgggcct cgaggtcgcg agaatgcggg cggcgaggag
     2641 gccggcgttc cggcccgcgt ccacggcgac cgtggccacg gggatccccg gcggcatctg
     2701 cacgatggag aggaggctgt ccaggccatc gagggtcttg gtcctgatcg ggacgccgat
     2761 gacgggcagg actgtcatgc tcgccgtcat gccgggaagg tgagcagagc cccccgcgcc
     2821 ggccacgatt acctcgaggc cgcggtaggc cgcgctcctg gcgtactcca cgaggcgcga
     2881 gggggtcctg tgggccgaga cgatggtcgt ctcgcaggga acgccgagct cggcgaggac
     2941 gcgcgaggtc tcggccatca ccgggagatc cga
//
LOCUS       DASSQV010000460         3332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1850100, whole genome shotgun sequence.
ACCESSION   DASSQV010000460 DASSQV010000000
VERSION     DASSQV010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850100"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFL04_08080"
     CDS             <1..658
                     /locus_tag="VFL04_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7839703.1"
                     /translation="TAFCVGLGLVIAVLADRSRFEKVYKAIIFMPMAISFVGAGVVWK
                     FIYAYKGEGVGLEEIGLLNAIVVALGGKAQAWLLYPGWNNFLLIAIMVWLQTGYAMVI
                     LSSAIKGIPAELLEAARIDGATEFQVFFRIIIPAIRGSIVTVTTTIVIFSLKLFDIVR
                     VMTGGNYGTNVIANEFYLEQFTYGNTGRASSIAIVLLVAVIPVMLYNLRQFSERKAFK
                     "
     gene            655..1767
                     /locus_tag="VFL04_08085"
     CDS             655..1767
                     /locus_tag="VFL04_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7839704.1"
                     /translation="MRKTGTAAGSLERRRGGFLVNLVLVLVCLVWIVPALGVLVTSFR
                     DSRDIFSTGWWTVLPHRAWVKTGEVKLDASVNLDADITIEGKSATFAEWREGVQVGPE
                     RRLQWYGNKRTLTLVAQEKRWVGFGANLTLDNYRGVLTGGEVRYKDANGDTITRQGNN
                     FSDAVLNSLAVAIPSTLIPILIAAFAAYAFAWMEFPGRKTLFVIVVALLVVPLQIALI
                     PVLRDFTKLGLNGQFLAMWLAHTGFGLPLAVYLLFNYISQLPRDIFESAFLDGATPFS
                     MFVRLVIPLSMPALASFAIFQFLWVWNDYLVALIFLGDRNRVVTSALAAMVGEKGQDW
                     HYLTSGAFLSMILPLIVFFSLQRYFVRGLMAGSVKG"
     gene            1843..>3332
                     /locus_tag="VFL04_08090"
     CDS             1843..>3332
                     /locus_tag="VFL04_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase"
                     /protein_id="HET7839705.1"
                     /translation="MSHVLESRSLNPSRLLLEETLFHCANGYLGVRGTFEEGYPDAWP
                     SVRGSYVNAFYDTHAIVHPERLFGFPETGEKIANVTDAQTIGLWAGGERVLAEPARVR
                     GYLRSLDIDRGTTRRSFVWRSSAGAELEVDIRRLVSLSTRELFAVEYRVAALGPGPAA
                     GAQPAEPLELELVLELDGDVENFFDPADPRVSGKAFKPLSVVASSCEERLGGGRLYVE
                     SRTAGSGLSLGVLSDFTCVGGRGLKVELRRGPGGAGARLRLGLGPGEEFRLTRMNVYA
                     DSARHGRVAEAVLAIAAALEGRPFDALVADQERELAAFWARAEVSIEGDPALDEGLRF
                     NLYQLLQSAPTDGASSIPAKGLSGEGYEGHYFWDAEIYMLPFFCYTNPPLARGMLLYR
                     HATLEGARAHAREMGQKRGAAFPWRTIAGRECSAYYPSGSAQYHISADVAYAVWRYYE
                     ATDDLGFLVEAGAELLFETARLWLELGHSSPEGFRIEAVTGPDEYT"
BASE COUNT          487 a         1222 c         1047 g          576 t
ORIGIN      
        1 cacggccttc tgcgtgggcc tcggcctcgt catcgccgtc ctcgccgacc gcagccgctt
       61 cgagaaggtc tacaaggcca tcatcttcat gcccatggcg atctccttcg tcggagccgg
      121 ggtcgtgtgg aagttcatct acgcctacaa gggcgagggg gtggggctcg aggagatcgg
      181 cctgctcaac gcgatcgtcg tggccctcgg gggcaaggcc caggcctggc tcctctaccc
      241 cggctggaac aacttcctcc tcatcgcgat catggtctgg ctccagaccg gctacgcgat
      301 ggtgatcctc tcctcggcga tcaagggcat ccccgccgag ctcctcgagg cggcgaggat
      361 cgacggggcc acggagttcc aggtcttctt ccgcatcatc ataccggcga tccgcgggag
      421 catcgtgacg gtcacgacga cgatcgtcat cttctcgctg aagctcttcg acatcgtgcg
      481 cgtcatgacg ggcggcaact acggcacgaa cgtcatcgcg aacgagttct acctcgagca
      541 gttcacctac gggaacacgg gcagggcgag ctcgatagcc atcgtcctcc tcgtggccgt
      601 catccccgtc atgctctaca acctccgcca gttctccgaa aggaaggcct tcaaatgagg
      661 aagacgggca ccgcagcggg cagcctcgaa cgacgcaggg gcggtttcct cgtcaacctc
      721 gtcctcgtcc tcgtctgcct cgtctggatc gtccccgccc tcggggtcct cgtcacctcc
      781 ttccgcgact cccgcgacat cttctccacg ggctggtgga ccgtcctccc ccaccgcgcc
      841 tgggtcaaga ccggcgaggt caagctcgac gcctcggtca acctcgacgc cgacatcacc
      901 atcgagggca agagcgctac cttcgccgag tggcgcgagg gggtccaggt ggggcccgag
      961 cggaggctcc agtggtacgg caacaagcgc accctgaccc tcgtggccca ggagaagcgc
     1021 tgggtcggct tcggcgccaa cctcaccctg gacaactaca ggggcgtcct caccggaggc
     1081 gaggtgcggt acaaggacgc gaacggggac acgatcaccc gccaggggaa caacttctcg
     1141 gacgccgtgc tcaactccct cgcggtggcc attccctcga ccctgatccc catcctcata
     1201 gcggccttcg ccgcctacgc cttcgcctgg atggagttcc cggggcgcaa gaccctcttc
     1261 gtcatcgtcg tggccctcct cgtcgtgccc ctgcagatcg ccctcatccc cgtgctgcgc
     1321 gacttcacca agctcggcct caacggccag ttcctcgcga tgtggctggc ccacacgggc
     1381 ttcggcctgc ccctcgccgt ctatctcctg ttcaactaca tcagccagct gccgcgggac
     1441 atcttcgagt cggccttcct cgacggggcc acgcccttct ccatgttcgt gcgcctcgtc
     1501 atccccctgt ccatgccggc cctggcctcc ttcgcgatct tccagttcct ctgggtgtgg
     1561 aacgactacc tcgtggccct catcttcctc ggcgaccgca accgcgtcgt gacgagcgcc
     1621 ctcgccgcga tggtgggcga gaagggccag gactggcact acctcacctc gggggccttc
     1681 ctgagcatga tcctgcccct catcgtgttc ttctcgctcc agcgctactt cgtgcggggc
     1741 ctcatggcgg gctcggtcaa gggctagagg cccgctcagc gatctccgcg atctccgcga
     1801 tctccgcgtc ctccgcggcg ggtccattac caggtggttc acgtgtccca tgtgctagag
     1861 tcgcgctccc tcaacccttc ccgtctcctc ctcgaggaga ccctcttcca ctgcgccaac
     1921 ggctatctcg gggtgagggg caccttcgag gagggctatc ccgacgcctg gccttcggtc
     1981 aggggcagct acgtcaacgc cttctacgac acccacgcga tagtccaccc cgagcggctc
     2041 ttcggcttcc ccgagacggg cgagaagatc gccaacgtga ccgacgcgca gaccatcggt
     2101 ctctgggccg gcggggagcg ggtcctggcc gagcccgccc gggtcagggg ctacctccgc
     2161 agcctcgaca tcgacagggg cacgacgagg cggagcttcg tctggcgctc ctcagccggg
     2221 gccgagctcg aggtcgacat caggcggctc gtgtccctgt ccacccgcga gctcttcgcc
     2281 gtcgagtacc gcgtcgccgc cctcgggcca gggccggcgg ccggggccca gcccgcggag
     2341 ccgctcgagc tcgagctcgt cctcgagctc gacggggacg ttgagaactt cttcgacccc
     2401 gctgacccgc gggtctcggg caaggccttc aagccgctct ccgtcgtggc cagctcctgc
     2461 gaggagcggt tgggcggagg caggctctac gtcgagtcga ggaccgccgg ctccggcctc
     2521 tcgcttggtg tcctctccga cttcacctgc gtgggcggca ggggcctcaa ggtcgagctc
     2581 cggcgggggc ccggcggggc cggggcaagg ctcaggctcg ggctggggcc gggggaggag
     2641 ttccgcctaa cgcgcatgaa cgtctacgcc gactcggcga ggcacggcag ggtggccgag
     2701 gcggtgctgg ccatcgccgc ggctctcgag ggccgcccct tcgacgccct cgtcgccgac
     2761 caggaaaggg agctcgccgc cttctgggcc agggccgagg tgtcgatcga gggggatccg
     2821 gctctggacg aggggctccg cttcaacctc taccagctcc tccaaagcgc gccgacggac
     2881 ggggcctcca gcatccccgc gaaggggctc tcgggcgagg gctacgaggg ccactatttc
     2941 tgggacgccg agatctacat gctccccttc ttctgctaca ccaatccccc gctggcccgc
     3001 ggcatgctcc tctaccgcca cgcgaccctc gaaggggccc gggcccacgc gcgggagatg
     3061 gggcagaaga ggggcgctgc cttcccctgg cggaccatcg cggggcggga gtgctcggcc
     3121 tattacccct cggggagcgc ccagtaccac atcagcgccg acgtcgccta cgccgtctgg
     3181 cgctactacg aggccaccga cgacctcggc ttcctcgtcg aggccggggc cgagctcctc
     3241 ttcgagacgg ccaggctctg gctcgagctc ggccactcct cgcccgaggg cttcaggatc
     3301 gaggcggtca cgggtcccga cgagtacacc tg
//
LOCUS       DASSQV010000461         4404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2347111, whole genome shotgun sequence.
ACCESSION   DASSQV010000461 DASSQV010000000
VERSION     DASSQV010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2347111"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFL04_08095"
     CDS             <1..285
                     /locus_tag="VFL04_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET7839706.1"
                     /translation="IFEEFKGTGNMEINLDRRLSDRRLFPAINIKKSGTRKEELLLSE
                     EELQKIWVLRKVINPMDDVGIMELLIDRMMKSKNNDAFLRSMNTPYNGSD"
     gene            420..638
                     /gene="rpmE"
                     /locus_tag="VFL04_08100"
     CDS             420..638
                     /gene="rpmE"
                     /locus_tag="VFL04_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET7839707.1"
                     /translation="MKKDIHPIYEMTNITCACGNVIETRSTVKNIQVEICSACHPFFT
                     GKQKLVDTAGRIERFNKKYGIKAAPEEK"
     gene            complement(740..1345)
                     /locus_tag="VFL04_08105"
     CDS             complement(740..1345)
                     /locus_tag="VFL04_08105"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HET7839708.1"
                     /translation="MELFVATGNAHKLTELGPAFPGHVLRTPADAGLRGFDVDEDGST
                     FLENALKKARSLHRLIGRPSIADDSGLVVTALGGEPGIYSARYGSPDGGQSKLSAAER
                     NAYLLARMEAHEDRSCAFVCCLVLMLSEDRFFVVQETCSGELLRAPRGEGGFGYDPVV
                     YLPALGRTVAELGVEEKNRLSHRGRAAARRCGILAELEASP"
     gene            complement(1377..2222)
                     /locus_tag="VFL04_08110"
     CDS             complement(1377..2222)
                     /locus_tag="VFL04_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839709.1"
                     /translation="MKDQSGLQELVVEYRRSGRGKDAIMERIAAQIYADPCRFGFDGE
                     DEAAEALLHYGHRIAALPDRFVDRGLPFEAYLATSLRFLSRTVRRNGRREAERRLVCE
                     RALAECPTGEDQGGPDPPSPTALRAAGKMGAGKGKRALAFASRLVFLYLKCAWDADDA
                     STRRVAEAAGVDLAWLSSATAQARRSLEDERARCERMTASRNRSWCRIRVLEARLMEE
                     TDLPRRIRLQASLERERSHFERAREELRLFRPVVPNSVVARILDVPKGTVDSGLYYLK
                     KGRVL"
     gene            complement(2471..3445)
                     /locus_tag="VFL04_08115"
     CDS             complement(2471..3445)
                     /locus_tag="VFL04_08115"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET7839710.1"
                     /translation="MLPVPRDLLDFIEQHDTFYVIGHKEPDGDCVGSQLALASYLRRS
                     GKRAVVCSAGPFTRTEILPYESRFTQIVPPEKASERAAAIILDCSSLSRIGLVAETLP
                     PVPLAFIDHHVSGEASGQVVYIDGTAPAVTLMVQLLIEALGGTPTKDEAELLFFGLGT
                     DTGFFRHLDEGSADTLAAASRLVAAGASPKRVFHLMYGGKSLFSSKLMGEILTRTEPH
                     FDGGLLVSFVTLEDQQRYGLASRDSDMLYQLLLSVSGCEAALIIRQESEKECTVGLRA
                     RDRVDVGSIAAQFGGGGHRLAAGLSVRGRLEEVRDRLLEAFAPYFEGD"
     gene            complement(3498..3911)
                     /locus_tag="VFL04_08120"
     CDS             complement(3498..3911)
                     /locus_tag="VFL04_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839711.1"
                     /translation="MKRIIVVFVLLALVAAGLAADTSAYYPVRLDVVKVYTHADGYRV
                     LFMSGYAGVKDTYLPIKWFQDSRDSSGQLLPPKAVLIRGGDPSFPYLVLFYKDGKFDH
                     LRLYVQDSLKDPMWGVMAPSEGVGKFDAQDLDIKF"
     gene            4001..>4404
                     /locus_tag="VFL04_08125"
     CDS             4001..>4404
                     /locus_tag="VFL04_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET7839712.1"
                     /translation="MERPLELRDRVAAVYDTLTPLWPDTAPLLHYRSCFELVCAVALS
                     AQCTDEQVNRVTPSLFAAWPDPESLSRAALPEVEELIHSLGFFRTKARHLVGMARILV
                     EDFGGEVPGTMDELLRLPGVGRKTANLVLAAC"
BASE COUNT          750 a         1425 c         1541 g          688 t
ORIGIN      
        1 atcttcgagg agttcaaggg cacgggcaac atggagatca acctggaccg caggctctcg
       61 gaccgccgcc tcttccccgc catcaacatc aagaagtcgg ggacgaggaa ggaggagctc
      121 cttctgtccg aggaggagct gcagaagatc tgggtcctcc gcaaggtcat caaccccatg
      181 gacgacgtgg ggatcatgga gctcctgatt gacaggatga tgaaatccaa gaataatgac
      241 gcgttcctga ggtcgatgaa cactccctac aacgggagcg actgatcggc tgaaggctca
      301 tgcgggcgcg cagtcgggtt gcgaaggcgc ggtcgcgtaa acggcgggcg atttaccggt
      361 cggcccgccg cttggccggt tatcaggcat tcggtacagc gcgcacggag gtaagtactg
      421 tgaagaagga catccatccc atctacgaga tgaccaacat cacctgcgcc tgcggaaatg
      481 tcatcgagac ccgttcgacg gtaaagaaca tccaggtcga aatctgctcg gcctgccatc
      541 ccttctttac cggaaagcag aaactcgtag acaccgcggg ccgcatcgag cgtttcaaca
      601 agaagtacgg catcaaggcc gcccccgaag agaaataggg gccagactcc cgtatcggaa
      661 aaagccgcac cctcgggtgc ggcttttcgc gtttggggcc agcggggccg cggaggcggg
      721 ctcgcggccc ccatgtccat cagggcgagg cttctagctc ggcgaggatc ccgcacctgc
      781 gggcggccgc gcggccgcgg tgcgaaagcc tgttcttctc ctcgacgccg agctcggcca
      841 ccgtccggcc cagagcgggg aggtagacca ccggatcgta gccgaagccg ccctcccccc
      901 tcggcgcgcg caggagctcg cccgagcagg tctcctggac gacgaagaac ctgtcctccg
      961 agagcatgag gacaaggcag cacacgaagg cgcaggagcg gtcctcatgg gcctccatcc
     1021 tggccaggag gtaggcgttc ctttcggcgg ccgagagctt gctctggcct ccgtcggggg
     1081 agccgtagcg ggcagagtag atgcccggct cgccgccgag ggccgtgacg acgaggcccg
     1141 aatcgtcggc gatggagggc cggccgatca gcctatggag gctccgggcc ttcttgaggg
     1201 cgttctcgag gaaggtcgag ccgtcctcgt ccacgtcaaa accccgcaga ccggcgtcgg
     1261 caggggtcct gagcacatgc ccgggaaagg ccggcccgag ctcggtgagc ttgtgcgcgt
     1321 tcccggtggc gacgaatagt tccatgccgg ccaccatagc cggaaggggc agggctctac
     1381 aagacgcggc ccttcttgag gtagtagagc cccgaatcca cggtgccctt gggcacgtcg
     1441 aggatcctgg cgacgaccga gttcggcacg acgggcctga agagcctgag ctcctccctc
     1501 gccctctcga agtggctgcg ctcgcgctcg agcgaggcct ggagcctgat gcggcgaggc
     1561 aggtcggtct cctccatgag cctcgcctcg agcaccctga tcctgcacca cgagcggttc
     1621 cggctcgccg tcatcctctc gcacctcgcc ctctcgtcct caagcgagcg cctcgcctgg
     1681 gccgtggccg agctgagcca ggcgaggtcg acccccgccg cctcggccac ccgtctcgtg
     1741 ctcgcgtcgt cggcgtccca ggcgcacttg aggtagagga agacgagccg gctcgcgaag
     1801 gcgagggcgc gtttgccctt ccccgccccc atcttcccgg cggccctgag ggccgtgggg
     1861 ctgggcgggt cggggccgcc ctgatcctcg cccgtcgggc actcggccag ggcgcgctcg
     1921 cagacgagcc tgcgctcggc ctcccgcctg ccgttgcggc ggacggtcct cgagaggaaa
     1981 cggaggctgg tggcgaggta ggcctcgaag ggcaggcccc tgtccacgaa ccggtcgggc
     2041 agggcggcga tgcggtggcc gtaatggaga agggcctcgg cggcctcgtc ctcgccatcg
     2101 aagccgaagc ggcagggatc ggcgtagatc tgggcggcga tcctctccat gatcgcgtcc
     2161 ttgccccggc ccgagcgccg gtactccacg acgagctcct ggagcccgga ttggtccttc
     2221 atggctgccc cccttcggcc ccgcagggtc tcggaaggga cattgcatgg accgtgccca
     2281 agcggccctg ggccgcgggc tgagggtggg gagggcgcaa agccctgagg gaagcgggcc
     2341 ccggatgggg aggccgcggg gctgggcggg gatgcggagc ccgggtcagg ggccgggctt
     2401 cctgtcttta cctccgctta cactcggcca gcccccagcc gggcgggact cgcccaggga
     2461 cggcgggcct tcagtcgccc tcgaagtagg gggcgaaggc ctcaagcagc ctgtccctga
     2521 cctcctcgag cctgccgcgg accgagagcc cggcggcgag cctgtggcct ccaccgccga
     2581 actgcgccgc gatgctgccg acgtcgacgc ggtcgcgggc gcggagcccc accgtgcact
     2641 ccttctcgga ctcctgtctg atgatcaggg cggcctcgca gcccgagacg ctgagcagga
     2701 gctggtagag catgtccgag tcccggctgg caaggccgta gcgctgctgg tcctcgaggg
     2761 tcacgaagga gacgaggagg cccccgtcga agtggggctc ggtccgggtc aggatctcgc
     2821 ccatgagctt gctggagaac agcgacttgc cgccgtacat gaggtggaag acccgcttcg
     2881 gcgaggctcc cgccgccacc agccgcgagg cggcggcgag ggtgtcggca gagccctcgt
     2941 cgaggtggcg gaagaagccc gtgtcggtcc cgaggccgaa gaagagcagc tctgcctcat
     3001 ccttcgtggg cgtcccgccg agggcctcga tgaggagctg gaccatgagg gtgaccgcgg
     3061 gggccgtgcc gtctatgtac acgacctggc cgctcgcctc ccccgacacg tggtggtcga
     3121 tgaaggcgag gggcacgggc ggcagggtct cggcgacaag accgatccgg ctgaggctcg
     3181 agcagtcgag gatgatggcg gccgcccgct cagaggcctt ctcgggcggc actatctggg
     3241 tgaagcggct ctcgtagggc aggatctcgg tgcgcgtgaa gggccccgcc gagcagacga
     3301 cggcgcgctt gcccgagcgt cggaggtagg acgcgagggc gagctgggag ccgacgcagt
     3361 ctccgtccgg ctccttgtgg ccgatgacgt agaaggtgtc gtgctgctcg atgaagtcca
     3421 aaaggtcgcg gggtaccgga agcatgggta acagcctttc tgcccgaaac ggagcgagga
     3481 gagttgggta tctgagccta gaacttgatg tcgaggtcct gggcatcgaa cttgccgacg
     3541 ccctccgagg gggccatgac gccccacatc ggatccttga ggctgtcctg gacgtagagg
     3601 cggaggtggt cgaacttgcc gtccttgtag aacaggacca gataggggaa gctcgggtct
     3661 ccgcccctta tgaggacggc cttgggaggc aggagctggc ccgagctgtc gcgggagtcc
     3721 tggaaccact tgatcggcag gtaggtgtcc ttgactccgg catagcccga catgaagagc
     3781 accctgtagc cgtcggcgtg ggtatagacc ttcacgacgt cgaggcggac cggatagtag
     3841 gccgaggtat cggcggcgag gccggcggcc accagagcga gaagcacgaa cacgacgatg
     3901 attcgtttca tgaattcccc cgttgcaggc aaagtatagc agtttcgaag gcctacgcaa
     3961 ggcgtggcga aaatccccgg gccggaatat actcccgcgc gtggaaagac ccttggagct
     4021 ccgtgaccgc gtggcggcgg tctacgacac cctcacgccc ctgtggcccg acacggcccc
     4081 cctcctccac taccgcagct gtttcgagct cgtctgcgcc gtggcccttt cggcccaatg
     4141 caccgatgag caggtgaacc gcgtgactcc atcgctcttc gcggcttggc ccgatcccga
     4201 gagcctgtcc cgggcggccc tgcccgaggt cgaggagctg atccacagcc tcggcttctt
     4261 ccgcaccaag gcgaggcacc tcgtcgggat ggcgcggatc ctcgtggagg atttcggcgg
     4321 cgaggtccca gggacgatgg acgagctcct gaggctcccg ggggtcggaa ggaagacggc
     4381 aaacctggtc ctcgccgcct gttt
//
LOCUS       DASSQV010000462         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1225916, whole genome shotgun sequence.
ACCESSION   DASSQV010000462 DASSQV010000000
VERSION     DASSQV010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225916"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2190
                     /locus_tag="VFL04_08130"
     CDS             <1..>2190
                     /locus_tag="VFL04_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7839713.1"
                     /translation="LAKEGGLVASWSEPILASDGRVLGLLAVYHGDPRPPGPDELRLY
                     SDSAALASLAIEGAWTQAELARHREGLERLVGQRTAELSQNELRTRTIIETVPDAVVV
                     IDQEGIIRTFNDAAARIFAVDAADAIGSPVSRLMPEPTRSAHSGYLRRFLETGENHAL
                     GRPIEFNACRADGSQFPIELIANEFRQGEARFFVASIRDITERKQDDEAIRRYVDEVA
                     DLYDNAPCGYHSLDEDGVFIRVNATELRWLGYRREELVGRRRITDLMDAEGRRQFEQG
                     YEGFKRRGFVNNLEYEIQRKDGSHFPVVINAVVQRDSRGDFLMTRATMTDNTERKRAE
                     ARLRESEERYRALFERGNDAAFVFETDEDGMPMSFTDVNVAACRQLGYTRDELLTMTK
                     NDINAPETALSLADHMKELETKDRSIFEVTHLTRDGRRIPVEVSATRTELGGRRCIIS
                     VGRDISDRKRAEAELHKLSLAVAQSPATIVITNRDGLIEYVNPAFTKTTSYTEADVLG
                     KNPRILKSGIHPKELYREMWRKILAEGEWRGDLCNRKKNGELFWESVSISAIRNEAGE
                     VTHYVAVKEDLSERKATEEATKDAKAEAETANRMKSEFLANMSHEIRTPMNAVVGFST
                     LALKTSLSPKQRSYISKIKRAGESLLGIINDILDFSKVEMGKMVLERVGFRIAELVED
                     VASMFEYGADSGGLDFHVSLAPDLPVCLMGDPLRIGQVLMNLVGNAI"
BASE COUNT          474 a          700 c          693 g          323 t
ORIGIN      
        1 cctggccaag gagggggggc ttgtcgcctc gtggtcggag cccatcctcg cctctgacgg
       61 ccgggtgctt ggcctcctcg cggtctacca tggcgatccg aggccgcccg gccccgatga
      121 gctcaggctc tactctgact cggccgccct cgcgagcctc gccatcgagg gggcctggac
      181 ccaagccgag ctggcccgcc accgcgaggg cctggagcgg ctcgtgggcc agcgcaccgc
      241 ggagctcagc cagaacgaac tcaggaccag aaccatcatc gagacggttc ccgacgctgt
      301 ggtggtgata gaccaggaag gcatcatcag gaccttcaac gacgcggccg cgaggatctt
      361 cgcagtcgat gctgccgatg ccatcgggag ccctgtctcg cggctcatgc ccgagcccac
      421 gaggagcgca cactcgggct atctccgccg cttcctggag acgggggaga accacgccct
      481 cggccggccc atcgagttca acgcatgcag ggccgacgga agccagttcc ccatcgagct
      541 gatagccaac gagttccgcc aaggcgaggc aaggttcttc gtagcctcga tcagggacat
      601 aaccgagcgc aagcaggacg acgaggcgat ccgcagatat gtcgacgagg tcgccgacct
      661 ctacgacaac gcgccctgcg gataccattc cctggatgag gacggggtct tcatccgcgt
      721 aaacgccacc gagctccgct ggctcggcta ccgccgcgaa gagctggtcg ggcgcaggag
      781 gatcacagac ctcatggacg ccgagggccg gcggcagttc gagcagggct acgagggctt
      841 caagcgccgg ggctttgtga acaacctcga gtacgagatc caaaggaagg acgggagcca
      901 cttccccgtc gtgatcaacg ccgtggtcca gagggacagc cggggcgatt tcctcatgac
      961 acgggcgacg atgacggaca acacggagcg gaaacgcgcg gaggcgaggc ttcgggagag
     1021 cgaggagcgc taccgggccc tcttcgagcg gggcaacgac gccgccttcg tgttcgagac
     1081 ggacgaggac ggcatgccca tgtcattcac cgacgtgaac gtcgccgcct gcagacagct
     1141 cggatacacc agggacgagc tgctcaccat gaccaagaac gacatcaacg ccccggagac
     1201 cgccctcagc ctggccgacc acatgaagga gctcgagacc aaggatcgga gcatcttcga
     1261 ggtgacccac ctcacgaggg acggcagacg cattccagtc gaggtcagcg cgaccaggac
     1321 ggagctcggc ggccgacgct gcataatctc cgtaggtcgc gacatatccg acaggaaaag
     1381 ggccgaggct gagctgcata agctttccct cgccgtggcc cagagcccgg ccacgatcgt
     1441 gatcacaaac cgggacgggt tgatcgaata cgtgaatcca gccttcacaa agaccaccag
     1501 ctacactgag gccgacgtcc ttggcaaaaa cccgaggatc ctgaagtcgg gcatccaccc
     1561 caaggagctg tatagggaga tgtggaggaa gatcctcgcc gaaggcgaat ggcgagggga
     1621 cctgtgcaac aggaaaaaga acggggagct cttctgggaa tccgtgtcga tctcggccat
     1681 ccgcaacgaa gccggagagg tcacccacta tgtcgccgtc aaagaggacc tgtcggagcg
     1741 caaggccacg gaggaggcca ccaaggacgc gaaagccgag gcagagacgg ccaaccgaat
     1801 gaaaagcgag ttcctcgcca acatgagcca tgagatccgc acgccgatga acgcggtcgt
     1861 cggcttctcg accctcgccc tcaagacctc cctgagcccc aagcagagga gctacatctc
     1921 aaagatcaag cgggctggcg agtccctcct cggcatcatc aacgacatcc tcgacttctc
     1981 gaaggtcgag atgggcaaga tggtccttga gcgggtcggc ttcaggatcg ccgagctggt
     2041 cgaggatgtc gcatcgatgt tcgaatatgg ggctgacagc ggcggcctgg atttccatgt
     2101 ctccctcgcc cccgacctgc cggtctgcct catgggcgat ccccttagaa ttggccaggt
     2161 tctgatgaac ctcgtcggca acgccatcaa
//
LOCUS       DASSQV010000463         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_854484, whole genome shotgun sequence.
ACCESSION   DASSQV010000463 DASSQV010000000
VERSION     DASSQV010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854484"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFL04_08135"
     CDS             complement(<1..223)
                     /locus_tag="VFL04_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839714.1"
                     /translation="MANTVTAARGKARLFVGLSRMTHSILDVAHPAIGGLLVFGGFPP
                     TPTILLGLGAAFAGFTAVFALNDVMDYRVD"
     gene            complement(216..1664)
                     /locus_tag="VFL04_08140"
     CDS             complement(216..1664)
                     /locus_tag="VFL04_08140"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7839715.1"
                     /translation="MAKPEPGPERVLFVRLPCKKVYPLGPIYLMSLVHRARPGLEQRL
                     LDLALVPRAQRTRRLQAEIEAFRPGLVAFSWRDIQIFSPQDSFGALRDAFVFFHDPSP
                     LRKIAAAFRGLWDIITYSSALAHNSGIIGRAVRAHPGLAMALGGPSVRIFGDRLAGRV
                     PKSVRIFADIGLGGFFGLLGIEEPQEEIEPGLDLGYIEEAFPQWPASRGEVIGVQSKR
                     GCPRGCLYCLYGHIEGRTVIRREPARVVDEIADYAERWSTSRIWFADAQLLSEAGDRA
                     HLVAILEGLLARGTKLAWSGYLRIDELDPELASLMVRSGLGELEVSLGSGSQAVVDEL
                     NLGVSVEGVLRGCELLAAAGYQGRIFLNLSLNAPGETKESLLETIAAVRRIKAIFGDS
                     RVLPLVFFLAIQPHTGLERRALADGHLKAGYDPLSVMPWNVLRLIYNPRPLGPLIGRA
                     CARGFAGDPEGVGERILAYIEAALAKTGGQHG"
     gene            1979..>2543
                     /locus_tag="VFL04_08145"
     CDS             1979..>2543
                     /locus_tag="VFL04_08145"
                     /inference="COORDINATES: protein motif:HMM:NF017933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF981 domain-containing protein"
                     /protein_id="HET7839716.1"
                     /translation="MFVDYLTVMLVNMIAGLVIMALFVYRYMDGDRKKVAPGLLMSGA
                     LALVTGLHMSLTWPLPGSYNIAFGEMAVLFGILFLFLGIASLADWELLTLAIYAVFAG
                     VASIVIGIRIFDLKMTSEPLISAGGFVLTGLAAVISLPVYYLRRFRLVRILASLALLG
                     GAAIWAAIGYLAYWAHLANFAKWAPDAM"
BASE COUNT          402 a          834 c          894 g          413 t
ORIGIN      
        1 ggtcgacgcg gtagtccatg acgtcgttga gggcgaagac ggcggtgaag ccggcgaagg
       61 cggcgccgag cccgaggagg atcgtcggcg tgggcgggaa accgccgaag accaggaggc
      121 cgcctatcgc gggatgggcg acgtcgagga tcgagtgggt catccgcgaa agcccgacga
      181 agagcctcgc cttccctctt gccgccgtca ccgtgttagc catgctgtcc tcctgtcttg
      241 gctagcgccg cttcgatgta ggcgaggatg cgctcgccga cgccttcggg gtctcccgcg
      301 aagcccctgg cgcaggccct cccgatgagg gggcccaggg ggcgggggtt gtagatcagc
      361 ctgaggacgt tccagggcat gaccgacagg gggtcgtagc ctgccttgag gtgcccgtcg
      421 gccagggccc gccgctcgag ccctgtgtgg ggctggatcg cgaggaaaaa cacgaggggc
      481 aggaccctgg agtccccgaa gatggccttt atcctgcgga ccgcggctat cgtctccagg
      541 agggactcct tggtctcgcc gggggcgttg agcgagaggt tgaggaagat cctcccctgg
      601 tagcccgccg cggccaggag ctcgcagccc cgcaggacgc cctcgaccga gaccccgaga
      661 ttgagctcgt cgaccacagc ctgggagccc gagccgaggg atacctcgag ctcgcccagg
      721 cccgagcgca ccatgaggga ggcgagctcg ggatcgagct cgtcgatcct gaggtagccc
      781 gaccaggcga gcttcgtgcc ccgcgcgagg agaccctcga ggatcgcaac caagtgggcc
      841 ctgtcgccgg cctcggagag gagctgggca tcggcgaacc agatccggga cgtcgaccaa
      901 cgctcggcgt agtcggcgat ctcgtcgacc accctggcgg gctcccgcct gatgaccgtc
      961 cgaccctcta tgtggccgta gaggcaatag aggcaccccc tcgggcagcc ccgcttggac
     1021 tggacgccga tcacctcgcc gcgggaggcc ggccactggg ggaaggcttc ctcgatgtag
     1081 ccgaggtcga ggccgggctc gatctcttcc tggggctcct cgatcccgag gagcccgaag
     1141 aagcccccga gcccgatgtc ggcgaagatc ctcacgcttt tcgggacccg tccggcgagg
     1201 cggtcgccga agatcctcac gctcgggccg ccgagggcca tcgcgaggcc ggggtgggcg
     1261 cgcacggccc ttcctatgat gccggagttg tgggcgaggg ccgagctgta ggtgatgatg
     1321 tcccagagcc ccctgaaggc cgcggcgatc ttccgcaggg gcgaggggtc gtggaagaag
     1381 acaaaggcgt ccctgagggc gccgaaggag tcctgggggg agaagatctg gatgtcccgc
     1441 caggagaagg cgacgaggcc ggggcggaaa gcctcgatct ccgcctggag ccggcgcgtc
     1501 ctctgggccc tcgggacgag ggcaaggtcg aggagcctct gctcgagccc cggacgggcg
     1561 cgatgcacga gggacatgag gtagatcggc cccaggggat agaccttttt gcagggcagg
     1621 cggacgaaga ggacgcgttc ggggcctggc tcgggctttg ccatgaggtg acgatactac
     1681 gacgcccgcg cggcctctgc aaggcgggcg gcgcgtcggt ccctgcccgg cccgcgattc
     1741 cagcctgctg gaccggcgcc ctcggggctc agggctcgaa atctccttgc ctcccgggct
     1801 ccaaggccgg atacttgatc aaggagctcc accatcgtgg atccgtggct ccccctcgtc
     1861 tttagccatg agaccgcggt ccctgccccg aagccggcca ggaccatgcg gcagtggata
     1921 taccaagccc cagccccgaa gcgggggcct gcgggctgga ctcgcgagga ggtctgcaat
     1981 gttcgtcgac tatctgaccg tgatgctcgt caacatgatc gccgggctcg tcatcatggc
     2041 cctctttgtc taccgctaca tggatggcga caggaagaag gtggctccgg gcctattgat
     2101 gagcggggca ctggccctgg tcacggggct tcacatgagc ctcacttggc cgctgccggg
     2161 gagctacaac atcgccttcg gcgagatggc cgtcctcttc ggcatcctct tcctcttcct
     2221 gggcatcgcc agcctggcgg actgggagct cctgaccctg gccatctatg cggtcttcgc
     2281 gggggtagcg tccatcgtga tcgggatcag gatcttcgac ctcaagatga cgagcgagcc
     2341 cctcatctcc gccggtggct tcgtcctgac gggtctcgcc gccgtgatct ccctgcccgt
     2401 ctactacctc cgcaggttca ggctggtcag gatcctggcc tccctggccc tgctcggggg
     2461 ggccgcgatc tgggctgcga tcggctatct cgcctactgg gcccacctcg ccaacttcgc
     2521 caagtgggct cccgacgcga tga
//
LOCUS       DASSQV010000464         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_729364, whole genome shotgun sequence.
ACCESSION   DASSQV010000464 DASSQV010000000
VERSION     DASSQV010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729364"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VFL04_08150"
     CDS             <1..1080
                     /locus_tag="VFL04_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7839717.1"
                     /translation="LLSCAQASRPTGPAARAVPVTVGLVAQKDFPVELTAIGHAESYQ
                     TAAVKPQVQGQLESVLFTPGQDVKAGAELFRIDPRAYEAALGKSEAILAKDQALETLA
                     RSDEAKTAELVKTGYDTQEQLDTAHANAQALKSQVDADRAQVDNDRLQLAYCTITAPI
                     SGRTGALQVDVGNIVSANSTVLVTINRLSPIYVSFSVPEGALAEVKRFSGTAGLRVQA
                     RVPGDGAAALEGRLVFIDNAVDSSTGTVLLKASFDNADGRLWPGQYANLMLTLNTERN
                     ATVIPTQALQTGQGGQFVFVVKGDRTVEMRPVTVARTIGSEAAIAAGLRPGETVVTDG
                     QLLLVPGAPVEIRDAARTAAASGRS"
     gene            1083..>2292
                     /locus_tag="VFL04_08155"
     CDS             1083..>2292
                     /locus_tag="VFL04_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7839718.1"
                     /translation="MNIAEIFIKRPVMTTLVMGAILLFGAVAFRLLPVNDLPTVDYPT
                     ISVNASLPGASPETMASSVATPLEQQFATIAGLASVTSTNFLGNTRITLQFDLDRNID
                     AAAQDVQAMIAKTARQMPPGMPSPPTYQKVNPAAAPILFLSMNSPTLPLSQIDDYAEN
                     VVAPRISMLSGVAQVNVFGAQKYAVRVQLDPMELARRQLGIDQVAASIAQANVNLPTG
                     TLVGAQRSYVITANGQLGNAASFRPLVVLYQNGQPVRLQELGQVIDSVENNQVAAWFV
                     DTRTIVMAIQRQPGTNTVQVVGSIKAILPSLRAQIPASVHLDVQYDRSVSILSSIGEV
                     ETTLVLAVLLVVIVIFAFLRNLTATIIPSLALPMAIVGTFAGMYAFGYTIDNLSLMAL
                     ILAVGFIVDDA"
BASE COUNT          391 a          884 c          679 g          338 t
ORIGIN      
        1 ttgctctcct gcgcgcaggc cagcagaccg acagggccgg ccgcgcgggc cgtccccgtg
       61 accgtgggcc tggtggccca gaaggacttc cccgtggagc tgacggccat cggccacgcc
      121 gagagctacc agacggccgc ggtgaagccc caggtccagg gccagctcga gtccgtcctc
      181 ttcaccccgg gccaggacgt gaaggcgggg gccgagctgt tcaggatcga cccccgcgcc
      241 tacgaggccg ccctcggcaa atccgaggcc atcctcgcca aggaccaggc cctcgagacc
      301 ctggcgcgca gcgacgaggc caagacggcc gagcttgtga agacgggcta cgacacccag
      361 gagcagctgg acacggcgca cgcgaacgcc caggccctca agtcccaggt cgacgctgac
      421 agggcccagg tcgacaacga ccgcctccag ctcgcgtact gcacgatcac ggcccccatc
      481 tcgggccgca cgggggcgct ccaggtcgac gtggggaaca tcgtctcggc caactccacg
      541 gtcctcgtca ccatcaacag gctctcgccg atctacgtga gcttctcggt gccggaggga
      601 gccctcgccg aggtcaagcg cttctcggga acggctgggc tcagggtcca ggccagggtt
      661 cccggggacg gggcagccgc cctcgagggc agactcgtgt tcatcgacaa cgccgtggac
      721 agctcgacgg gcaccgtcct gctcaaggcc agcttcgaca acgccgacgg caggctctgg
      781 cccggccagt acgcgaacct gatgctcacc ctgaacaccg agaggaacgc cacggtcatc
      841 cccacccagg ccctccagac gggacagggc ggccagttcg tcttcgtcgt caagggcgac
      901 aggacggtgg agatgcggcc cgtcaccgtg gcgcgcacga tcgggagcga ggcggccatc
      961 gccgccggcc tcaggccggg cgagaccgtc gttaccgacg gccagctcct cctcgtcccc
     1021 ggcgcccccg tcgagatcag ggacgcggcc aggaccgcgg ccgcctcggg tcggagctga
     1081 gcatgaacat agccgagatc ttcataaaaa ggcccgtcat gaccaccctt gtgatgggcg
     1141 ccatcctcct tttcggcgcc gtcgccttcc ggctcctgcc ggtgaacgac ctgcccaccg
     1201 tggactatcc cacgatctcg gtgaacgcga gcctgccggg ggccagcccc gagacgatgg
     1261 cctcatccgt ggcgaccccc ctcgagcagc agttcgccac aatcgccggt cttgcctcgg
     1321 tcacctcgac caatttcctc ggcaacacga ggatcaccct ccagttcgac ctcgaccgga
     1381 acatcgacgc ggctgcccag gacgtccagg ccatgatcgc caagaccgcc cgtcagatgc
     1441 cgccgggcat gccgagccca cccacctacc agaaggtgaa ccccgccgcc gcccccatcc
     1501 tcttcctcag catgaactct cccaccctcc ccctctccca gatcgacgac tacgcggaga
     1561 acgtcgtcgc cccccgcatc tcgatgctga gcggggtggc ccaggtgaac gtcttcgggg
     1621 cgcagaaata cgcggtcagg gtccagctcg accccatgga gctcgcgagg cgccagctcg
     1681 gcatcgacca ggtcgccgcc tcgatcgccc aggccaacgt aaacctcccc acggggaccc
     1741 tcgtgggcgc ccaacgctcc tatgtcatca ccgccaacgg ccagctcggc aacgccgcct
     1801 ccttccggcc ccttgtcgtg ctctaccaga acggccagcc ggtgcggctc caggaactcg
     1861 gacaggtcat cgactcggtc gagaacaacc aggtggccgc ctggttcgtc gacacgagga
     1921 ccatcgtcat ggcgatccag cgccagccgg gcaccaacac ggtccaggtc gtcggctcga
     1981 tcaaggccat cctgccctcg ctgcgggccc agatcccggc ctcggtccac ctcgacgtgc
     2041 agtacgaccg ttcggtctcg atcctctcgt cgatcgggga ggtggagacc accctcgtcc
     2101 tcgccgtcct cctcgtggtg atcgtgatct tcgccttcct gcgcaacctc accgccacca
     2161 tcatcccgag cctcgccctt cccatggcca tcgtgggcac cttcgccggc atgtacgcct
     2221 tcggctacac catcgacaac ctttccctca tggccctcat cctcgccgtc ggcttcatcg
     2281 tcgacgacgc ca
//
LOCUS       DASSQV010000465         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1226515, whole genome shotgun sequence.
ACCESSION   DASSQV010000465 DASSQV010000000
VERSION     DASSQV010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226515"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFL04_08160"
     CDS             complement(<1..180)
                     /locus_tag="VFL04_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET7839719.1"
                     /translation="MIDKLQAISARLRELDDMVANPALVRDQNRYREIMRERSRLAVI
                     DEAFAEYRRVLSGLED"
     gene            complement(182..352)
                     /locus_tag="VFL04_08165"
     CDS             complement(182..352)
                     /locus_tag="VFL04_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839720.1"
                     /translation="MKVEINPRGNGACPICVANGNCPLQQRLGGSLASMNGTDSDMEL
                     VIYSCPYFKEKA"
     gene            528..>2438
                     /locus_tag="VFL04_08170"
     CDS             528..>2438
                     /locus_tag="VFL04_08170"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET7839721.1"
                     /translation="MKVSKFGGSSVGSPEAIARMVGIVAERGPRLIVVSAFSGVTDEL
                     LATAESAAAGEEGWGEGLESLARRHRSAVAALAPAQACGPVDLLLDELAELLRGVRLS
                     RELSPRGRDLVMSFGERLSAPIVEAAFRAAGLAAEAVDARSLIIAAGGFGKGRPLMAE
                     TRQAALARLGAFARQPEGPAPMPIVTGFIASTRDGETITLGRGGSDYTATILGSVLGA
                     EEVEIWTDVNGMMTADPRRVPDSFSLESLSYAEAIELSHFGARVIYPPTIQPALEAGI
                     PIRILNSLEPSFPGTVIRADAPPSRYPVRGVTSISSIALLRLQGPGMVGLVGAASRLF
                     GCLARRGIGVVLISQGSSEQSICFAVQKGERVDAVEAILEEFESELASGAIERPVAEG
                     DKAILAVVGERMKRRPGISGRVFQALGRNGINVSAIAQGSSELNISAVVDAEDESKAL
                     AAVHEAFFLSGTRSANVLLVGCGLIGGTLLGQIARHRTLLAEDFSVRLNVIGIADSRR
                     MIMSARGIALEDWAARLEREGEASHLDAFLARARSMKLPNTVFVDCTANGELPALYPS
                     ALRYAMAVVTPNTKGNSGPLSAYRELLATARSSGSPYLYETTAGAGLPVISTLHDLQV
                     SGDRITRIEASLS"
BASE COUNT          357 a          864 c          851 g          366 t
ORIGIN      
        1 gtcctcaagg ccggagagga cgcgcctgta ctcggcgaag gcctcatcta tgacggcgag
       61 gcgggagcgc tcgcgcatga tctcgcggta gcggttctgg tccctgacga gggccgggtt
      121 ggcgaccatg tcgtcgagct cgcggaggcg ggccgagatg gcctggagct tgtctatcac
      181 gctaggcctt ttccttgaag taggggcagg agtagatgac gagctccatg tcggaatccg
      241 tcccgttcat cgaggcgagg ctgccgccca gacgctgctg cagggggcag ttcccgttcg
      301 cgacgcagat cgggcaggcg ccgttccctc gggggttgat ctccaccttc atgcatggat
      361 ggtatccgaa agccgccggc cgcggcaata cggcggccaa gcctgaggga tcaaagcttg
      421 gcggcgtcaa ggacttaagc tcggcggagc ttgacggagc ttgacggcgc ctcggccggg
      481 ggctaggatg ccgggattcc gtccgtagct atcgggagaa acgcggcatg aaggtctcga
      541 aattcggggg gagctcggtc ggcagccccg aggcgatcgc gaggatggtc ggcatcgtgg
      601 ccgagcgggg gccgaggctc atcgtcgtct cggccttctc gggtgtgacc gacgagctcc
      661 tggccaccgc cgagtccgct gctgccggcg aggagggctg gggcgaaggg ctcgagtccc
      721 tcgcgaggcg gcaccggtcg gccgtggcgg ccctcgcccc cgcccaggcc tgcggccccg
      781 tcgacctcct cctcgacgag ctcgccgagc tcctccgcgg cgtccggctc agccgcgagc
      841 tctcgccccg cggccgcgac ctcgtgatga gcttcggcga gaggctctcg gccccgatcg
      901 tcgaggccgc cttcagggcg gcgggcctgg ccgccgaggc agtcgatgcc cgctccctca
      961 tcatcgcggc gggcgggttc gggaagggca ggcccctcat ggccgagacg aggcaggccg
     1021 ccctggcccg gcttggcgct ttcgcccgcc agcccgaggg gccggccccc atgccgatcg
     1081 tcaccggctt catcgcctcg accagggatg gcgagaccat caccctgggc aggggcggct
     1141 cggactacac ggccacgatc ctgggctcgg tcctcggcgc cgaggaggtc gagatctgga
     1201 ccgacgtgaa cggaatgatg acggccgatc cgcgccgcgt gccggacagc ttcagcctcg
     1261 agtccctcag ctacgccgag gccatcgagc tctcgcactt cggcgccagg gtgatctacc
     1321 ccccgaccat ccagcccgcc ctcgaggcgg gcatcccgat caggatcctg aacagcctcg
     1381 agccctcctt ccccggcacg gtgatcaggg ccgacgcccc gccctcgcgc taccccgtca
     1441 ggggcgtgac ctcgatctcc tcgatcgccc tcctcaggct ccagggcccg gggatggtcg
     1501 ggctggtcgg cgcggcgagc aggctcttcg gctgcctcgc gcgacggggc atcggcgtcg
     1561 tcctcataag ccagggctcg agcgagcagt cgatctgctt cgccgtccag aagggcgagc
     1621 gcgtggacgc ggtcgaggcc atcctcgagg agttcgagtc cgagctcgcc tcgggggcca
     1681 tcgaaaggcc cgtggccgag ggcgacaagg ccatcctcgc cgtggtcggc gagaggatga
     1741 agcgcaggcc cgggatctcg ggccgggtct tccaggccct cgggcgcaac ggcatcaacg
     1801 tctccgcgat cgcccagggc tcgagcgagc tcaacatctc ggccgtcgtc gacgccgagg
     1861 acgagtccaa ggccctcgcg gccgtccacg aggccttctt cctctcgggg acgaggtcgg
     1921 ccaacgtcct cctcgtgggc tgcggcctca tcggcggcac cctcctcggc cagatagcca
     1981 ggcacaggac cctcctcgcc gaggacttct cggtgcggct caacgtgatc gggatcgccg
     2041 actcgaggcg gatgatcatg agcgcccgcg gcatcgccct cgaggactgg gccgcccggc
     2101 tcgagcgcga gggcgaggcc agccacctcg acgccttcct ggccagggcc cgcagcatga
     2161 agctgcccaa cacggtcttc gtcgactgca ccgcgaacgg ggagctcccc gccctgtacc
     2221 cgagcgccct gcgctacgcg atggccgtcg tgaccccgaa cacgaagggg aactcgggcc
     2281 ccctgtccgc ctacagggag ctcctggcca cggcgcggag ttcgggatcg ccctacctct
     2341 acgagaccac ggccggcgcc ggcctgccgg tgatctcaac cctgcatgac ctccaggtct
     2401 cgggcgacag gatcacgagg atcgaggcga gcctctcg
//
LOCUS       DASSQV010000466         5456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1226559, whole genome shotgun sequence.
ACCESSION   DASSQV010000466 DASSQV010000000
VERSION     DASSQV010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226559"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..571)
                     /locus_tag="VFL04_08175"
     CDS             complement(95..571)
                     /locus_tag="VFL04_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7839722.1"
                     /translation="MESQALTKRLNLSFEARFGLVSAILERARAFMRDADQGEHYGIL
                     VVVRELVENAIVHGNRSDPTKLVKLAIRLCAPGLFEVQVEDEGQGFDYAEVVARLPGG
                     ANGEERSGYLLIHELADKVAFENHGSKVRVLARTDGARNSGSRDPKVPLRPEEERI"
     gene            complement(770..2167)
                     /locus_tag="VFL04_08180"
     CDS             complement(770..2167)
                     /locus_tag="VFL04_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7839723.1"
                     /translation="MSPAARRSPRIIVVDDEAYILASVESALRSAGIAEITTLSDSSL
                     LMGELEKDDVAIVLLDLSMPKLSGEELLPRIILEHPDVSIVIVTGNRDLERAIQCIKA
                     GAEDYLVKPVERTRLVATVRRLVELQELRLENAEIRDRLLEGRVRRREAFAALVTADP
                     RMEAIMGYAEAIAPSAQPVLITGETGVGKELFARAIHELSGRKGEFVAVNAAGLDDTF
                     FSDLLFGHRAGAFTGATTNLDGLADRAKDGSLFLDEIGDLGRQSQLKLLRFIETGEYY
                     PLGSDLLRRSRARVIVATNRDLEAGIAAGDFRRDLYFRLRNHRIRIPPLRERRGDLPL
                     LVGHFLELASKELGREVPATPRELFELLGRHGFPGNVRELKALVVDALSTSGPGLLSI
                     EVFREAAGSATPREPGETLAMSFPSTLPSLKEVTELLVDEALRRAGGNQSVAAGLLGV
                     SPQAISKRLKSRPSI"
     gene            complement(2164..3753)
                     /locus_tag="VFL04_08185"
     CDS             complement(2164..3753)
                     /locus_tag="VFL04_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7839724.1"
                     /translation="MRDAGETATEDMKARLELLLQTLPVGISVLDATGRIILHNRRLE
                     RILRLSSEDFARGAYAGIAFLRSDGSEIPKEELPWARVFAGERAAEGVELQFEHQGSR
                     VQLEVSALACEAPGWAVIIVTRDVSSRWRAEAGREASEARFRLAIDGAPLPIMIHDDE
                     GRILTVNKAWTGATGYSPEDIPSIEAWASLAYGEGRPGLLERIRRNLDTTTTIHVGLV
                     KLRRKDGSTAYLDFSRAPLGSLPSGRRGTIVMAIDTTDRIEAERVADERRRQLVQSEK
                     LAALGSLVAGVAHEVNNPNHTIALNASILAEVWESARPVLDEALAGREDSLLGGIEYR
                     ELRARAPALLGGIAEASSHIEQIVRGLRDFARSEEERVIDEVNLNLVVKASATLLQNL
                     IRQSTRAFSLELLEDLPPVLGSFHQLEQVVINLVQNACQALYDPSQAVVVSTTYDAAS
                     NRVRLEVRDEGRGLNPEELARAKDPFFTTKRKTGGMGLGMSISNSIVEEHGGSLELRA
                     EPTGGARATVELPVAPREVDR"
     gene            3915..4868
                     /locus_tag="VFL04_08190"
     CDS             3915..4868
                     /locus_tag="VFL04_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839725.1"
                     /translation="MDSPSIRVGSPRRFLTPEVERVSRRIGRVFSALEELPYLGEAEL
                     EDYRYFAGLFADSLSRIDGRARAGRLRPLDLRGFPQCLRYPGYAPRIGVLIGSFDPFQ
                     MTHLAMALRFLACDAAEADLVFVVPEGAPDPRKPGRTEYRFRYEILKRQLAGVLDPLV
                     VPLDIGEDADTIEIVRRLIALHPGAAIRLTHIVGSDTLPTALRFLPDDLEAWGATAKE
                     QRVSLDFSVFVIRRESRTGARPFVEAARRLGVRSVVDRMIVGAPSSTAFRSARAITVV
                     LPTEAILSRLELLFRYGLHRPWSGPGGEGGEAAASQPDYEI"
     gene            complement(4944..>5456)
                     /locus_tag="VFL04_08195"
     CDS             complement(4944..>5456)
                     /locus_tag="VFL04_08195"
                     /inference="COORDINATES: protein motif:HMM:NF026866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 23 domain-containing
                     protein"
                     /protein_id="HET7839726.1"
                     /translation="PFEIRYEWICYRDSWGTSQLGGRLEFVWQTGSGSLGLAFHNDLF
                     GLLVRDEYRTAALELASGFRALGADCSASVGMRLWTGSTWGQGRLHWGQSYAMTLHPP
                     GAGYSAGILYAGFRWGMVRLELGWDSEGIRDFIQNGTHRIIDDGKVPLVDRAARPYIA
                     LSLFPDGGLY"
BASE COUNT          777 a         1866 c         1921 g          892 t
ORIGIN      
        1 tcgagcctca tgatctggga gatggcgaag cccccgaccg cggccgcgat gagcgcgacg
       61 ccaagaaagc cggcgatcag cttgacacct attttcatat cctttcctcc tctgggcgca
      121 gtgggacttt ggggtcgcga gacccgctat tcctcgctcc gtcggtgcgg gcgaggactc
      181 gaaccttgct gccgtgattc tcgaaggcca ccttgtccgc gagctcatgg atgaggaggt
      241 agcccgagcg ttcctctccg ttggcgccgc cgggcagcct cgccaccact tcggcgtagt
      301 cgaagccctg cccctcgtcc tcgacctgga cctcgaagag gccgggcgcg cagagccgta
      361 tcgcgagctt gaccagcttc gtggggtcgg agcggttgcc gtgcacgatc gcgttctcga
      421 cgagctcccg caccacgacg aggatgccgt agtgctcgcc ctggtcggcg tcgcgcatga
      481 aagccctggc gcgctcgagg atcgccgaga cgaggccgaa tctggcctcg aagctgaggt
      541 tgagacgctt ggttagggct tggctttcca tgccacaaga aatgcaggga ccatgccaaa
      601 catcggtccc ttgcatgagt ccttgtatgg caagaagatg gccgacgacc gccccgcggc
      661 cccttgaatg ggctctcgca accagggttg ccccgggccg gcgggatcta gctatgtggc
      721 ggtccgggaa ggccttagct cgagatcaac caaagttgtc gaatcaacct cagatggagg
      781 gccgggattt cagccttttg ctgatggcct gggggctcac gccgaggagg cctgcggcca
      841 cgctctggtt ccctcccgcc cgcctgaggg cctcgtcgac gaggagctcg gtgacctcct
      901 tcaggctggg aagggtcgag gggaagctca tggcgagggt ttcgccgggc tcccgcgggg
      961 tggcggagcc agcggcctcc ctgaagacct cgatcgagag gaggccgggg ccgctcgtgc
     1021 tcagggcgtc gacgaccagg gccttgagct cccgcacgtt gcccgggaag ccatgacggc
     1081 cgaggagctc gaagagctcg cgaggcgtgg cggggacctc ccttcccagt tccttcgacg
     1141 cgagctcaag gaagtgcccc acgaggaggg gaaggtcgcc ccggcgctcg cgcagggggg
     1201 ggatcctgat gcggtggttg cggagacgga aatagaggtc cctccggaaa tcgcccgcag
     1261 cgatgcccgc ctcgaggtcc cggttcgtcg cgacgatgac gcgggcgcgg ctccgcctta
     1321 ggaggtccga gccgagggga tagtactccc cggtctcgat gaagcggagg agcttgagct
     1381 gggactggcg gccgaggtcg cctatctcgt cgaggaagag gctcccgtcc ttcgcgcggt
     1441 cggcgaggcc gtcgaggttc gtggtggctc cggtgaaggc cccggcgcgg tggccgaaaa
     1501 gcaggtcgct gaagaaggtg tcgtcgaggc cggcggcgtt gacggcgacg aactcgccct
     1561 tgcggccgct cagctcgtgg atggcccggg cgaagagctc cttgcccacc cccgtctcgc
     1621 cggtgatgag gacgggctgg gccgaggggg cgatcgcctc ggcgtagccc atgatggcct
     1681 ccatgcgcgg gtcggcggtc accagggccg cgaaggcctc gcggcgcctc accctgccct
     1741 cgaggaggcg gtcgcggatc tcggcgttct cgagcctgag ctcctgcagc tcgacgaggc
     1801 gccgcaccgt cgccacgagc ctcgtgcgct cgacgggctt cacgaggtag tcctcggccc
     1861 cggccttgat gcactggatc gcgcgctcga ggtcccggtt gcccgtgacg atgacgatgg
     1921 agacgtcggg gtgctcgagg atgatgcgcg ggaggagctc ctcgccggag agcttgggca
     1981 tgctcaggtc gaggaggact atggccacat cgtccttctc gagctcgccc atgaggaggg
     2041 aggagtcgga gagggtcgtg atctcggcga tgcccgcgga gcggagcgcg ctctcgacgc
     2101 tcgcgaggat gtaggcctcg tcgtcgacga cgatgatgcg cggggaacgc cgcgccgccg
     2161 ggctcatcgg tccacctccc tcggcgcgac ggggagctcc acggtcgccc tcgcgccgcc
     2221 cgtgggctcg gccctgagct cgaggctgcc cccgtgctcc tcgacgatgc tgttcgagat
     2281 gctcatgccg aggcccatgc cccccgtctt gcgcttcgtc gtgaagaagg ggtccttcgc
     2341 gcgggcgagc tcctcggggt tgaggccccg gccctcgtcg cgcacctcga gccgcacccg
     2401 gttcgaggcg gcatcgtacg tggtcgagac gaccacggcc tgggaggggt cgtagagggc
     2461 ctgacaggcg ttctggacga ggttgatgac cacctgctcg agctggtgga agctgccgag
     2521 gacggggggc aggtcctcga ggagctcgag cgagaaggcc ctcgtcgact gccgtatgag
     2581 gttctgcagg agggtggccg aggccttcac gacgaggttg aggttgacct cgtcgatgac
     2641 ccgctcctcc tcgctccgcg cgaagtcgcg cagcccgcgc acgatctgtt cgatgtggct
     2701 cgaggcctcc gcgatgccgc cgaggagggc gggggcccgc gccctgagct cgcggtactc
     2761 gatcccgccg aggagggagt cctccctgcc cgcgagggcc tcgtcgagga cgggcctggc
     2821 cgattcccag acctcggcca ggatgctcgc gttgagcgct atcgtgtggt tggggttgtt
     2881 gacctcgtgg gccacgccgg ccacgaggga gccgagggcg gcgagcttct ccgactgcac
     2941 gagctggcgc cggcgctcgt cggccacgcg ctcggcctcg atgcggtcag tcgtgtcgat
     3001 ggccatgacg atcgtgcccc gcctcccgct gggcaggctc ccgagggggg cgcggctgaa
     3061 gtcgaggtag gccgtgctcc cgtccttccg cctgagcttg acgaggccca cgtggatggt
     3121 cgtcgtggtg tcgaggttcc tccgtatccg ctcgaggagg cccggcctgc cctcgccgta
     3181 ggcaaggctg gcccaggcct cgatgctcgg gatgtcctcg ggggagtagc cggtcgcccc
     3241 ggtccaggcc ttgttgacgg tgaggatcct gccttcgtcg tcgtggatca tgatggggag
     3301 gggcgcgcca tcgatcgcga ggcggaaccg ggcctcgctc gcctcgcgcc ccgcctcggc
     3361 ccgccagcgc gagctgacgt cccgcgtgac gatgatcacc gcccagcccg gagcctcgca
     3421 ggcgagggcc gagacctcga gctggacccg ggacccctgg tgctcgaact ggagctcgac
     3481 accctcggcc gcccgctcgc cggcgaagac cctggcccag ggcagctcct ccttcgggat
     3541 ctcgctgccg tcggagcgga ggaatgcgat gccagcatag gccccgcggg cgaagtcctc
     3601 gctcgagagc ctgaggatcc gctcgagcct gcggttgtgg aggatgatcc tgcccgtcgc
     3661 gtcgagcacg gagatcccga cgggaagggt ctggagaagg agctcgagcc tggccttcat
     3721 gtcttcggtg gcggtttcgc ctgcatcccg cattttgtct ttgtcctctc ggggtccccg
     3781 tccacccgcg ccggcggcga cccgcatctt agtctactgt aggaggggcg gcttgcaagg
     3841 gcccagactg gcggcgccct acactggcgc tggcggcctt gtccccgcgc attcggcgac
     3901 tatacttcat ctgcgtggat tccccctcga tcagggtcgg ctcgccgcgc cgcttcctca
     3961 cgcccgaggt cgaacgggtc tcgagacgga tcgggcgcgt cttctcggcc ctcgaggagc
     4021 tgccctacct cggcgaggcc gagctcgagg actaccgcta tttcgccggg ctcttcgccg
     4081 actcgctctc ccgcatcgac gggagggcga gggcgggcag gctccgcccc ctcgacctcc
     4141 gcggctttcc ccaatgcctg cgctaccccg gctacgcgcc gaggatcggc gtcctcatcg
     4201 gctccttcga ccccttccag atgacccacc tcgccatggc cctgcgcttc ctcgcctgcg
     4261 acgcggccga ggcagacctg gtcttcgtcg tgcccgaggg ggcgcctgac ccccgcaagc
     4321 ccggcaggac cgagtaccgc ttcaggtacg agatcctcaa acggcagctc gctggcgtcc
     4381 tcgaccccct cgtggtcccc ctcgacatcg gcgaggacgc cgacacgata gagatcgtgc
     4441 gccgcctcat cgccctccac cccggggcgg ccatcaggct cacccacata gtcggctccg
     4501 acaccctccc cacggcgctc cgcttcctgc ccgacgacct cgaggcatgg ggcgccaccg
     4561 cgaaggagca gagggtgagc ctcgacttct ccgtcttcgt catcaggcgc gagagccgca
     4621 cgggggcaag accctttgtg gaggcggccc gccgcttagg cgtgcggagc gtcgtcgacc
     4681 ggatgatcgt cggcgccccc tcgagcacgg ccttccgctc ggcgcgcgcc atcaccgtgg
     4741 tcctccccac cgaggccatc ctctcccggc tcgagctcct cttccgctat ggcctgcacc
     4801 ggccctggag cggcccgggc ggagaggggg gcgaggccgc ggccagccag cccgactacg
     4861 agatctaggg cctgaggccg gccaaggccg ccgcgcgccg ggcccggagc cttctcgccc
     4921 tatggtcggc gtcggcgggg ccctcagtac agaccgccgt cggggaagag gctcagggcg
     4981 atgtagggcc gggccgccct gtccacgagg gggacctttc cgtcgtcgat gatgcggtgg
     5041 gtcccgttct ggatgaagtc gcggatcccc tcggaatccc agcccagctc gagcctgacc
     5101 atgccccatc tgaagccggc gtagaggatg cccgcggagt agccagcccc cgggggatgg
     5161 agggtcatcg cgtagctctg tccccagtgg aggcggccct gaccccaggt cgagccggtc
     5221 cagagcctca tgccgaccga ggccgagcag tcggcgccca gggccctgaa cccggaggcc
     5281 agctcaaggg ccgccgtccg gtactcgtcg cgcacaagga ggccgaagag gtcgttgtgg
     5341 aaggcaaggc cgagggagcc cgagccggtc tgccagacga actcgagcct tcccccgagc
     5401 tggctcgtgc cccagctgtc gcggtagcag atccattcgt agcggatttc gaaggg
//
LOCUS       DASSQV010000467         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1352949, whole genome shotgun sequence.
ACCESSION   DASSQV010000467 DASSQV010000000
VERSION     DASSQV010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352949"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1724)
                     /locus_tag="VFL04_08200"
     CDS             complement(180..1724)
                     /locus_tag="VFL04_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839727.1"
                     /translation="MNQVAGSSRSTAALRYRDEGLAASQRELDALAARGAAAAVLEKR
                     ILEAFASKDRAIHNHDWQALDPRTIEAEFLMPRKEAFHFALTNWTRHIFLPGLKPAVA
                     DKLLMFGLGRIFSTYNDVGVQRNTDADVNLVVGDAMTDADLAYLARRLGLFAQAMRER
                     FGVTIEIDREFTLLRLKTVMANLADSDPARRRKCELFYKSVSNSIHVFQDNASIRGRI
                     FSRVKALPDTELFEHFLGLGSARPSFMKIRKDAEPLPIVVDGSGEKVVAWNVIGSQAF
                     AKYWRVALPRTVFVSPPDWVFSMKYFVNRVYDYVCAMRNCGYDLRRIGFDAGCDGSLD
                     PDYLFVREAHKLMLYLQELNELSTCSFGVKGDYSYVSRRRMRRFMELGGEKFVADFES
                     MVLGGGMIQGSNRTKYLELRQKVVAKARDRLIEGPIDGYGRLPAGLDYELVFKDDNEY
                     RICLPYTWADLAFFAFSVIAARMSQIVENRLVPALRGLGRASPPLLATKEQEGKGGSR
                     RDDHPG"
     gene            complement(1883..2266)
                     /locus_tag="VFL04_08205"
     CDS             complement(1883..2266)
                     /locus_tag="VFL04_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839728.1"
                     /translation="MTKTEDLGFCVLEFRQGFMVATIQPDSPIGEAQTLAVAGAKRGH
                     YGEERVGLVLYNPWNSPIDPGIFAHAERLMEEQGYAFLIVARPNPSNSRMLELFNKHC
                     LVYVGDSLEDAVAHAETVVRAASCT"
BASE COUNT          414 a          758 c          777 g          414 t
ORIGIN      
        1 gacacgatgt cgccgactga aggccaaatc cactcagccc gaaaggcggt caccagtgac
       61 ggtgaccgct tttttgtttg cgccgacaaa tccactggag cccgacgaag cggttaccga
      121 gggcccggaa catcgagcca ggatggcgcg gcgcgctagc gaccacgaca atccattccc
      181 tagcctggat ggtcgtcgcg gcgggagccg cctttgcctt cctgctcctt ggtggccagg
      241 agaggagggc tcgcccggcc taggcccctg agcgccggga cgaggcggtt ctctacaatc
      301 tgggacatcc tggccgctat gaccgagaag gcgaagaagg ccaggtcggc ccaggtgtag
      361 ggcagacaga tgcggtactc gttgtcgtcc ttgaagacga gctcgtagtc caagcccgcg
      421 gggaggcggc catagccgtc gatggggccc tcgatcaggc ggtccctcgc cttcgccacg
      481 accttctgtc tcagctcgag gtacttcgtc cggttgctcc cctggatcat gcccccgccg
      541 aggaccatgc tctcgaagtc ggcgacgaac ttctcgccgc cgagctccat gaagcgccgc
      601 atcctccgcc tcgagacgta ggagtagtcc cccttgaccc cgaaggagca ggtggagagc
      661 tcattgagct cctggaggta gagcatcagc ttgtgggcct cgcgcacgaa gaggtaatcc
      721 gggtcgaggc tgccgtcgca gcccgcgtcg aagccgatcc tccgcaggtc gtagccgcag
      781 ttgcgcatcg cgcagacata gtcgtagacg cggttcacga agtacttcat cgaaaaaacc
      841 cagtcgggtg gggacacgaa taccgtccgc gggagggcga cccgccagta cttcgcgaag
      901 gcctgggatc cgatgacgtt ccaggcgacg accttctcgc ccgagccgtc caccacgatg
      961 ggcaggggct cggcgtcctt cctgatcttc ataaatgagg gcctcgcgct tccgaggccc
     1021 aggaaatgct caaagagctc ggtgtccggg agggccttga ccctggagaa gatcctcccc
     1081 cggatggacg cgttgtcctg gaagacatgg atgctgttcg agacgctctt gtagaaaagc
     1141 tcgcacttcc tgcgcctcgc agggtcgctg tcggccaggt tcgccatgac cgtcttcagc
     1201 ctgaggaggg tgaactccct gtcgatctcg atggtgacgc cgaagcgctc gcgcatggcc
     1261 tgggcgaaga gcccgagcct cctcgcgaga taggccaggt cggcatcggt catcgcgtcg
     1321 cccacgacca ggttcacgtc cgcgtcggtg ttcctctgga ccccaacgtc gttgtaggtc
     1381 gagaagatcc tccccagtcc gaacatgagg agcttgtccg cgaccgccgg cttgaggccg
     1441 ggcaggaaga tgtgcctggt ccagttggtg agggcgaagt ggaaggcttc cttgcggggc
     1501 atcaggaact cggcctcgat ggtccggggg tcgagggcct gccagtcgtg gttgtggatc
     1561 gccctgtcct tcgaggcgaa ggcctcgagg atccgcttct cgaggaccgc cgccgcggcc
     1621 ccccgcgcgg cgagggcgtc gagctccctt tggctcgcgg cgaggccctc gtcccggtag
     1681 cggagcgccg ccgtgctgcg tgacgagccg gccacctggt tcatctcggc ttggacccca
     1741 taagttatat aataatatca ggttcagccc gaaaatcaag ccggaccgcc ccccggggcg
     1801 ccgcgagagg gccgaagctg ggagactgcg gctgaggggc cgcggccgag gggcccgcgg
     1861 atttgcccgc cgcggccccg gcctaggtgc agctggccgc cctcaccacc gtctcggcgt
     1921 gcgccaccgc gtcctccagg ctgtccccga catagaccag gcagtgcttg ttgaagagct
     1981 caagcatcct gctgttgctg ggattgggtc tggcgacgat gaggaaggca tagccctgct
     2041 cctccatgag ccgctccgcg tgcgcgaaga tccccgggtc gatgggggag ttccaggggt
     2101 tgtagagcac gaggccgacg cgctcctcgc cgtagtgtcc cctcttcgcg ccggccaccg
     2161 caagggtctg ggcctcgcct atgggagagt ccggctggat ggtcgcgacc atgaagccct
     2221 ggcggaactc aaggacgcag aagccgaggt cctcggtctt ggtcatgatc cctccacagg
     2281 agaatcatgg caaaggatac ccatatacga cagaaatgat caagatttcc ttccggaacc
     2341 ggccatggcc ggggcccgcg gct
//
LOCUS       DASSQV010000468         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_980637, whole genome shotgun sequence.
ACCESSION   DASSQV010000468 DASSQV010000000
VERSION     DASSQV010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980637"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFL04_08210"
     CDS             <1..248
                     /locus_tag="VFL04_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839729.1"
                     /translation="LSFSTDYSNFDSHGLPQTLINRYYTPGSPPTLVATETVMVSNTY
                     DSDGYQSGDTESASYVPNGGGPTESHSYAYVVTWTQY"
     gene            363..986
                     /locus_tag="VFL04_08215"
     CDS             363..986
                     /locus_tag="VFL04_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7839730.1"
                     /translation="MLELFAKGGTVLYILVILSGLAVAIIIERLLYFRRISTDEAKLY
                     SRVKSSLEKGHYDEAMAICDQNLSPFSALMRVGIENRAYPEQVQKEVLKDAAAQEVPR
                     LEKYVAALGTIANIAPLIGLLGTVTGTMRALGVLGSFGAVADPSILASGVSEALINTV
                     GGIIVAVFSVIFYNYLVSRVNLTILKLEGQVSTLVLMINSGPAKGEE"
     gene            991..1410
                     /locus_tag="VFL04_08220"
     CDS             991..1410
                     /locus_tag="VFL04_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7839731.1"
                     /translation="MRFARRRLEHAVNINLVPLIDVLLLLIIFFMITTTFKLTPGISL
                     TLPSSSTAQTVQSSVIRVVAVSEAEIYVDRTRTDLKGLPAVIKKRAGAGDPAALKAML
                     EGDKNSSYQLMVSVLDALRRNGIESVGLRTRVEKGAP"
     gene            1407..>2077
                     /locus_tag="VFL04_08225"
     CDS             1407..>2077
                     /locus_tag="VFL04_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839732.1"
                     /translation="MRSDWVRAQDRRRRTLAVLFTVGLYGLAILGLWIVSLISPFGLA
                     TSPGPVMIDLAGPEGGLALGSPSAPPRPEGQAPSPPAPPPAPAAKTAPGGVKVAVPAP
                     PSAAPAVSAPPSASPGALPPEKAAPAPNPPPNPAPQATAPAGSRTFTSSPGSSGAQAA
                     PGPAGAAGSSGAGLSGGTGTVTYPGTDMGNAIATTFGGASGKVGRNLYAPVYMFMPPP
                     QAISD"
BASE COUNT          322 a          804 c          632 g          319 t
ORIGIN      
        1 agctgtcctt ctcgacggac tactcgaact tcgactcgca cggcctgccc cagaccttga
       61 tcaacaggta ctacactccc ggctcgcccc cgaccctggt ggccaccgag actgtcatgg
      121 tgagcaatac ctacgactcc gatggctacc agagcgggga taccgaatct gccagctacg
      181 tgcccaatgg cggcgggccc accgagtccc acagctacgc ctacgtcgtg acctggaccc
      241 agtactaggc gccgacgtcg gatccagtcg tccgaccgtt gcctaactcc ctcctccgcg
      301 ttaccatctg aagagcggcc gcggcttccc aagccgcgcc gcgctttcgt ccaggaggaa
      361 ccatgctgga actgttcgcg aagggcggca ccgtcctcta catcctcgtg atcctgtcgg
      421 gcctcgccgt ggccatcatc atagagcgcc tcctctactt ccggcgcata tcgacggacg
      481 aggcgaagct ctactcgagg gtgaagtcga gcctcgagaa ggggcactac gacgaggcca
      541 tggccatctg cgaccagaac ctctcgccct tctcggccct catgcgcgtg gggatcgaga
      601 accgggccta ccctgagcag gtgcagaagg aggtcctcaa ggacgccgcg gcccaggagg
      661 tcccccgcct agagaagtac gtcgctgccc tcgggaccat cgccaacatc gcccccctca
      721 tcggcctcct cgggacggtc acgggcacga tgcgcgccct cggggtcctc ggcagcttcg
      781 gcgccgtggc cgacccctcg atcctggcca gcggggtctc cgaggccctg atcaacacgg
      841 tgggcggcat catcgtggcg gtcttctcgg tcatcttcta caattacctc gtgagcaggg
      901 tcaacctgac catcctgaag ctcgagggcc aggtgagcac cctcgtcctc atgatcaact
      961 cgggcccggc caagggcgag gagtgaggcc atgaggttcg cgcggcggag gctcgagcac
     1021 gcggtcaaca tcaacctcgt gcccctcatc gacgtccttc tgctcctcat catcttcttc
     1081 atgatcacga cgaccttcaa gctcaccccc ggcatctccc tcaccctgcc gagctcgtcc
     1141 acggcccaga ccgtccagtc ctcggtgatc cgggtggtcg cggtctccga ggccgagatc
     1201 tacgtggaca ggacgaggac agacctcaag ggcctgcccg ccgtcatcaa gaagcgggcg
     1261 ggggcgggcg accccgccgc cctgaaggcg atgctcgagg gcgacaagaa ctcgtcctac
     1321 cagctcatgg tctcggtcct cgacgcgctc aggcgcaacg gcatcgagtc cgtcggcctc
     1381 cgcacccgcg tggagaaggg ggcgccgtga ggtcggactg ggttcgcgcc caggaccgcc
     1441 gccgccgcac cctcgcggtg ctcttcaccg tggggctcta cggcctcgcc atcctggggc
     1501 tgtggatcgt gagcctcatc tcccccttcg gcctcgccac cagccccggt cccgtcatga
     1561 tagacctcgc gggccccgag ggagggctcg ccctcggctc gcccagcgct cccccgcgtc
     1621 ccgagggcca ggccccgagc ccgcctgctc ccccgcccgc ccccgccgcg aagacggcgc
     1681 cgggtggggt gaaggtggcg gtgccggctc cgccctcagc ggctcccgcc gtctcggcgc
     1741 cgccgtccgc gagccccggc gcccttcctc ccgagaaggc ggccccggcc cccaatccgc
     1801 cgccgaaccc cgccccccag gcgacggccc ccgccggctc gaggaccttc accagctcgc
     1861 ctggctcctc cggggctcag gctgccccgg gcccagcagg agccgccggc tcctccggcg
     1921 ccgggctcag cggtggcacg ggcaccgtga cctaccccgg caccgacatg ggcaacgcga
     1981 tcgccacgac cttcgggggg gcctcgggca aggtgggcag gaacctctac gccccggtct
     2041 acatgttcat gcccccgccc caggcgatca gcgacga
//
LOCUS       DASSQV010000469         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_606301, whole genome shotgun sequence.
ACCESSION   DASSQV010000469 DASSQV010000000
VERSION     DASSQV010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606301"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VFL04_08230"
     CDS             <1..1005
                     /locus_tag="VFL04_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7839733.1"
                     /translation="VRLELSRAEGRGEGAQTLPLLISVSDTGIGMPPEKVESAFERFT
                     QLDGSSTRRAGGAGLGLDIVRKSLESIGGRVEVDSAPGRGTRVTAHLSLGLPEGDAES
                     AREEALRARRLVITGFGEEEGGDLEELASSMGAECLFADRLEEAAAMRGLPLVICDER
                     ILHRGGGGSLALPEKSLAALSRRLIVAVRFGSPCRAWTGFPEGVAFATMPLRAGRLSD
                     AVAALGRPAEERTAGPEGTAAAGAQDAEQRGLLLDFAEALEEASRSGIAGEAERIAKE
                     MSKDCARLGFREGERLAFSALLATRKGEAAGLARFALRARSLAGLCQGSAGKTDYAEG
                     "
     gene            1002..1412
                     /locus_tag="VFL04_08235"
     CDS             1002..1412
                     /locus_tag="VFL04_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7839734.1"
                     /translation="MMRILVVEDDFGSRRMMQKLLAPYGDIDVVVDGEEAVNAFRLAW
                     EEKEPYGVVFMDIMMPKMDGHEALKRMRAMERELGVRPAKEAKVIMTSVLDDPKNVIE
                     AYYEGAATSYLLKPIDRDKLSAELSRLGIAAPKE"
     gene            1419..>2843
                     /locus_tag="VFL04_08240"
     CDS             1419..>2843
                     /locus_tag="VFL04_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7839735.1"
                     /translation="MPGIAVVEDEYIVALDIRNFLERSGYSVVGVYASGDDLLANCEA
                     IKPDLVLMDIKIRGSLDGVETARIVYERHRVPVVLLTAYADDETVARAKITHPFGYIL
                     KPFEERELRTAIEIALYRSSMEKRLRESEERYRRLFTEGISGNFLADSSGRVTEANPA
                     FMRLMGILPGEALPHQESLFPERLAWESFREGLVGARRLELWELSLVDRGGREVHVLA
                     NAVALFDTEGRFTGIQGELTDTTERRKLEERLLQAQKMEAIGRLAGGVAHDFNNILTA
                     VLGYANLLTDEIGDSKEARDDVEGIRNAAARAANLTRQLLAFSRRQPFAPKTFDLNTL
                     IQDAERLLRRLLTEEIALNLELTAGPAWILADPVQIEQVLLNLAVNAKDSMPEGGDIT
                     IGTRIEVLEAPKTVGSDTLAPGGYALIEVRDDGEGIPPEIRDKIFEPFFTTKPKDRGT
                     GLGHRPRQLVLREGLGEEAAAGLGV"
BASE COUNT          493 a          953 c          985 g          412 t
ORIGIN      
        1 gtcaggctcg agctcagccg cgccgagggg aggggcgagg gagcccagac ccttcccctc
       61 ctcatctccg tcagcgacac cgggatcggc atgcccccgg agaaggtcga gtcggcattc
      121 gagcgcttca cccagctgga cggctcgagc acgcggcggg cgggcggggc ggggctcggc
      181 ctcgacatcg tgcgcaagag cctcgagagc atcggcggca gggtcgaggt cgacagcgcc
      241 cctggccgag gcacgcgggt cacggcccac ctcagcctcg gcctgcccga aggcgatgcg
      301 gagtcggcca gggaggaggc gctgcgggcg aggaggcttg tcatcacggg cttcggcgag
      361 gaggaaggcg gcgatctcga ggagctggcc tcctctatgg gcgccgaatg ccttttcgcg
      421 gatcgcctcg aggaggccgc cgccatgcgc ggcctccccc tcgtcatatg cgatgagagg
      481 atcctccatc ggggcggggg agggtcactg gccctccccg agaagagcct cgcggccctg
      541 tcccgccgcc tcatagtggc cgtccgcttc ggcagcccct gccgcgcctg gacgggtttc
      601 ccggagggcg tcgccttcgc caccatgccg ctcagggcgg ggcgtctgtc cgacgcagtg
      661 gcggccctcg ggaggccggc tgaggagagg acggcggggc ctgaggggac ggcagcggct
      721 ggggcgcagg acgccgagca gcggggcctc ctcctcgatt tcgccgaggc cctcgaggag
      781 gccagcagga gcgggatcgc cggcgaggcc gagaggatcg cgaaggagat gagcaaggac
      841 tgcgccagac tgggcttcag ggaaggcgag cgcctcgcct tctcagccct cctcgcgacc
      901 aggaagggcg aggctgccgg cctcgcccgc ttcgccctgc gggcaaggtc gctcgcgggt
      961 ttgtgccagg gatctgcagg caaaacggat tacgcggagg gatgatgcgc atactcgtgg
     1021 tggaagacga tttcggcagc cgtcggatga tgcagaagct cctcgctccc tacggggaca
     1081 tcgacgtcgt ggtcgacgga gaggaggcgg tcaacgcctt ccgcctggcc tgggaggaga
     1141 aggagcccta cggcgtggtg ttcatggaca tcatgatgcc caagatggac ggccacgagg
     1201 ccctgaagcg gatgcgcgcg atggagcgtg aactcggcgt caggccggcg aaggaggcga
     1261 aggtcatcat gacctcggtg ctggacgacc ccaagaacgt gatagaagcc tattacgagg
     1321 gcgcggccac cagctatctg ctcaagccga tcgacaggga taagctgagc gctgagctct
     1381 cgaggctggg gatcgcggcg ccgaaggagt aggcaagcat gccgggcatc gcggtcgtcg
     1441 aagacgaata catcgtcgct ctcgatatcc gcaacttcct cgagcggagc ggctattccg
     1501 tcgtcggcgt gtatgcctcg ggggatgacc tcctcgccaa ctgcgaggcc atcaagcccg
     1561 acctcgtcct catggacatc aagatcaggg ggagcctcga cggggtcgag accgccagga
     1621 tcgtctatga gcgccaccgc gtccccgtcg tcctcctcac ggcctatgca gacgacgaga
     1681 ccgtcgcccg ggccaagatc acccacccct tcggttacat cctcaagcca ttcgaggagc
     1741 gcgagctcag gacggcgatc gagatcgccc tgtaccgctc gagcatggag aagcggctgc
     1801 gggagagcga agagcgctat aggcgacttt tcaccgaggg catctcgggc aatttcctcg
     1861 ccgattcatc gggcagggtc accgaggcga atcccgcctt catgcgcctc atgggcatcc
     1921 tcccggggga ggccctgccc caccaggaat cgctcttccc cgagcgcctc gcctgggagt
     1981 ccttccggga gggcctcgtg ggcgcgcgcc gcctcgagct ctgggaactc tccctcgtgg
     2041 accggggggg ccgggaggtc cacgtcctcg ccaacgccgt ggccctcttc gacaccgagg
     2101 gccgcttcac ggggatccaa ggcgagctga ccgacaccac ggagcgccgc aagctcgagg
     2161 agcgtctgct ccaggcccag aagatggagg ccatcggccg cctcgccggg ggcgtcgccc
     2221 atgacttcaa caacatcctg accgccgtgc tcggctacgc caacctcctc accgacgaga
     2281 tcggggacag caaggaggcg agggatgacg tggagggcat ccgcaacgcc gccgcccgcg
     2341 cggcgaacct cacgcgccag ctcctcgcct tctcaagacg gcagcccttc gcccccaaga
     2401 ccttcgatct caataccctc atccaggacg ccgagcgcct tctgcgcagg ctcctgaccg
     2461 aggagatcgc gctcaatctg gagctcacgg ccggacccgc ctggatcctc gcggatcccg
     2521 tgcagatcga gcaggtcctc ctcaatctcg ccgtcaacgc caaggactcg atgcccgagg
     2581 gcggggacat cacgatcggc acgaggatcg aggtcctgga ggccccgaag accgtcggct
     2641 cggataccct ggccccgggc ggctacgccc tgatcgaggt gagggacgac ggcgagggaa
     2701 ttccgcccga gatcagggac aagatcttcg agcccttctt caccacaaag cccaaggaca
     2761 ggggtacggg cctcggccac cgccctcgcc agctcgttct ccgtgaaggg cttggcgagg
     2821 aagcggccgc tggcctcggg gtc
//
LOCUS       DASSQV010000470         3745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2099390, whole genome shotgun sequence.
ACCESSION   DASSQV010000470 DASSQV010000000
VERSION     DASSQV010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099390"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFL04_08245"
     CDS             complement(<1..541)
                     /locus_tag="VFL04_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839736.1"
                     /translation="MEEKANAPNGAWGPLYAVGAASAAAYVLMVLVPLVLMATEPLAP
                     LAGGAAILEYIAAHRAIYLAELICFVGLSLPALLVFLALGAALWQRARSRALMGAAVG
                     VGSELIALALGSSPPSLNGGLWVLAGRYEAAADPALRSSLTAAAEALAAWANAVSAAG
                     ILTALAMLLLSIPLAKAGFP"
     gene            complement(594..917)
                     /locus_tag="VFL04_08250"
     CDS             complement(594..917)
                     /locus_tag="VFL04_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7839737.1"
                     /translation="MAANGAPDEHCPSEALRLLGDYTTLRIIDFLSGSALRFTDLRRV
                     LDDTNVPTLVARLKRMTKAGLVTRSEATLDGKSVTYELTEDGKALLPVLRAIQSFAQR
                     TRSRA"
     gene            1018..1641
                     /locus_tag="VFL04_08255"
     CDS             1018..1641
                     /locus_tag="VFL04_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HET7839738.1"
                     /translation="MASTTVSTEKLLETTAAAVREHGIAAEILGTRAEALERIKALIP
                     AGASVMTGASVSLKEIGFEDYLISGGHPWRNLKAGIVAETDPAKQQALRRQALLADYF
                     LGSVHALVETGELVIASATGSQLGPYAFAASNVIWVVGSQKIVPSLDAAIARIKEHIM
                     PHEELRMRAMTGGKMGTMLGKLMVIHREAPFLGRKLSLLLIREPTGD"
     gene            complement(1604..2443)
                     /locus_tag="VFL04_08260"
     CDS             complement(1604..2443)
                     /locus_tag="VFL04_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839739.1"
                     /translation="MASDPGILSLDDRRFAALDEAERRFTPADFGSFFEVRQLLGEPG
                     PGPGPEARAQLRRLCSRSFGLNAGGLTEACKDRFFEILFDGARFADPRPAGDAAGMAG
                     AASLAGAASVAGAASVGHASGAAREGQVAEDLLRPILLELAAFPRRRGDFALPFSFAS
                     KLVSARLESSPIYDRHVLAFFGERPPPARVERETRIAWYLALLGRIGESYRAWAGEGR
                     VEALLGRFRSRFAGLEGTHVVRLLDFLVWKAGAEGLIGGSGEGGRGRAGPSPPWAPGS
                     GGG"
     gene            complement(2452..3195)
                     /gene="pssA"
                     /locus_tag="VFL04_08265"
     CDS             complement(2452..3195)
                     /gene="pssA"
                     /locus_tag="VFL04_08265"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HET7839740.1"
                     /translation="MRIPRAALPSALTLAGLFFGFCAIVRIVGGDVLGGLWLIVLSGV
                     FDVADGAAARLVRATSRFGAELDSLADIVCFGVAPSLLLYEVSLKAMGPAGLLLSSLP
                     TLCGCFRLARFNVQTGPDDKTGFVGLPIPPAAVVLCSFVVLCLDSTTGLGALGRAVLP
                     WLVVLVCGLMVSRLRYPTVPRPSLEGIRKEPLKSTLMALGVVAVIAGGWRAIFPVMSC
                     FALAPLFRALTQAARRLPALLWPARAAGR"
     gene            complement(3207..>3745)
                     /locus_tag="VFL04_08270"
     CDS             complement(3207..>3745)
                     /locus_tag="VFL04_08270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7839741.1"
                     /translation="SGYPPSAFGLTLRGAGRVILEAIGWTLPLIALLVAFKLVLILFV
                     PGFRGRGLIDPADLVARLGKGELGLYLLFLLLYTLFSPIQEFIARGCIQSSFRNFLPD
                     TRRSIWISIILSNLLFSMAHSHLNLGFAIVAFVPGLFWGWMYERQRSLLGVCVSHVLL
                     GDLAIYVLGFSSSFFARQ"
BASE COUNT          672 a         1205 c         1427 g          441 t
ORIGIN      
        1 gcgggaaacc tgccttggcg agggggatcg agaggaggag catggccagg gccgtgagga
       61 tgccggcggc cgagaccgcg ttggcccagg ccgccagggc ctcggcggcc gcggtgaggg
      121 agcttcgcag ggcggggtcg gctgccgcct cgtagcgccc agcgaggacc caaaggcctc
      181 cgttgagcga gggcgggctc gagcccaggg ccagggcgat gagctcggag ccgaccccga
      241 cggccgcgcc catgagggcg cggctccgcg cgcgctgcca gagcgcagcg cccagggcga
      301 ggaagacgag gagggcaggc agtgacaggc ccacgaagca gatgagctcg gcgaggtaga
      361 tggcgcggtg ggcggcgatg tactcgagga tggccgcccc tccggcgagg ggggcaaggg
      421 gctcggtggc catgagtacg aggggcacga ggaccatgag gacataggcc gcggccgagg
      481 cggcgcccac ggcgtacagg gggccccagg ccccattggg ggcattcgct ttttcttcca
      541 tgccgggagg ataggccagg tcgaagccgc tggcaatgag cggcgcgctg gccctaggcc
      601 cgagacctcg tgcgttgggc gaagctctgg atcgcccgca ggacggggag gagggccttc
      661 ccgtcctcgg tgagctcata ggtcaccgat ttgccgtcga gggtggcctc ggagcgggtg
      721 acgaggcctg ccttggtcat gcgtttgagc ctcgcgacga gggtggggac gttggtgtcg
      781 tcgaggaccc tcctgaggtc ggtgaagcgg agggccgagc ccgagaggaa gtcgatgatc
      841 ctcagcgtgg tgtagtcgcc gaggagcctc agggcctcgg agggacagtg ttcgtcaggc
      901 gccccgttcg cagccatgcc tcaagcatac cgctttcgcg ggcccggcgg cattggtaaa
      961 aagttttgac taataatcct gcccttcgta gatttcgctc ctaaggagaa tatgcgaatg
     1021 gcaagtacca cagtctcaac ggagaagctg cttgagacca cggcggcggc ggtcagggag
     1081 cacggcatcg ccgccgagat cctgggcacg agggccgagg ccctggagcg gatcaaggcc
     1141 ctgatcccgg ccggggcgag cgtcatgacc ggggcctcgg tcagcctcaa ggagatcggc
     1201 ttcgaggact acctcatatc ggggggccac ccctggagga acctcaaggc gggcatcgtg
     1261 gccgagaccg acccggcgaa gcagcaggcc ctgcgccggc aggccctcct cgccgactac
     1321 ttcctgggca gcgtccacgc cctggtcgag accggggagc tcgtcatcgc gagcgcgacc
     1381 gggagccagc tcggccccta cgccttcgcc gcctcgaacg tgatctgggt ggtcggctcg
     1441 cagaagatcg tccccagcct cgacgcggcc atcgcgcgca tcaaggagca catcatgccc
     1501 cacgaggagc tgcgcatgag ggccatgacc gggggcaaga tgggcaccat gctcggcaag
     1561 ctcatggtga tccaccgcga ggcccccttc ctcggcagga agctcagcct cctcctgatc
     1621 cgggagccca cgggggacta ggccccgccc ggccgcggcc cccttccccg gacccgccga
     1681 tgaggccctc ggccccggcc ttccagacca ggaaatccag gaggcgcaca acgtgggtcc
     1741 cctcgagccc tgcgaagcgg gagcggaagc gccccagcag ggcctcgacg cggccctccc
     1801 cggcccaggc gcgatagctc tccccgatcc ggcccaggag ggccagatac caggctatcc
     1861 tcgtttcgcg ctcgaccctg gccgggggcg gcctctcgcc gaagaaggcg aggacatggc
     1921 ggtcgtagat cgggctcgac tcgagccggg ccgagacgag cttggaggcg aaggagaagg
     1981 gcagggcaaa gtcgccgcgt cgccgcggga aggccgcgag ctcgaggagg atcggacgga
     2041 gcaggtcctc ggccacctgg ccctccctcg ccgcgccact cgcgtgaccc acgctcgccg
     2101 cgccggccac gctcgccgcg ccggccaagc tcgccgcgcc ggccatgccg gccgcgtccc
     2161 cggcggggcg cgggtcggcg aagcgcgccc cgtcgaacag gatctcgaaa aagcggtcct
     2221 tgcaggcctc ggtgaggccc cccgcgttga gcccgaagga gcgcgagcag agcctgcgca
     2281 gctgggcccg cgcctcggga ccggggcccg gccccggctc gcccaggagc tgtctgacct
     2341 cgaagaagga gccgaagtcg gcgggagtga agcgcctctc ggcctcgtca agggcggcga
     2401 agcggcgatc gtcgaggctg aggatgcccg gatccgaagc caagccccgg ctcatctgcc
     2461 agcggcccgc gccggccaga ggagggcagg gagccgccgc gccgcctggg tgagggcgcg
     2521 gaacagggga gcgagggcga agcaggacat gacggggaag atggccctcc acccgcccgc
     2581 gatgaccgcc accaccccca gggccatgag ggtcgactta agtggctcct tcctgatccc
     2641 ctcgaggctc ggcctgggca ccgtggggta gcgcagccgg ctcaccatga ggccgcagac
     2701 caggaccacg agccagggca ggacggcccg gcccagggcg ccgagccccg tggtcgagtc
     2761 aaggcagagg acgacgaagg agcacaggac cacggcggcc ggggggattg ggaggccgac
     2821 gaagccggtc ttgtcgtcgg gaccggtctg gacgttgaag cgcgcgaggc ggaagcagcc
     2881 gcagagggtg ggcagcgagc tcaggaggag gcccgctggc cccatggcct tgagcgagac
     2941 ctcgtagagc aggagggagg gcgcgacgcc gaagcagacg atgtcggcca gggagtcgag
     3001 ctcggcgccg aagcggctcg tggcccgcac gagcctcgcg gcggccccgt cggcgacgtc
     3061 gaagacgccc gagaggacga tgagccagag ccctccaagg acgtcccccc cgacgatcct
     3121 tacgatcgcg cagaagccga agaagaggcc tgccagggtc agggctgagg gcagggcggc
     3181 gcgcgggatc ctcatggctc gccgcctcac tgccgcgcga agaatgagct cgagaagccc
     3241 aggacgtaga tcgcgaggtc cccgaggagg acatgggaga cgcagacacc gaggagggac
     3301 ctctggcgct cgtacatcca gccccagaac agcccgggca cgaaggcgac gatggcgaag
     3361 cccaggttga ggtgggagtg ggccatggag aaaaggaggt tcgagaggat gatcgagatc
     3421 cagatcgagc ggcgcgtgtc cggcaggaag ttgcggaacg aggactggat gcagccgcgg
     3481 gcgatgaact cctggatggg cgagaagagg gtgtacagga ggaggaagag gaggtagagg
     3541 cccagctcgc ccttgccgag cctcgccacg aggtcagcgg gatcgatgag gcccctgccg
     3601 cggaagccgg gcacgaagag gatgaggacg agcttgaagg cgacgaggag ggcgatgagg
     3661 gggagggtcc agccgatggc ctcgaggatg acccgacccg cgcccctcaa ggtgagcccg
     3721 aaggcgctcg ggggatagcc gcttt
//
LOCUS       DASSQV010000471         3955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1101976, whole genome shotgun sequence.
ACCESSION   DASSQV010000471 DASSQV010000000
VERSION     DASSQV010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101976"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFL04_08275"
     CDS             complement(<1..401)
                     /locus_tag="VFL04_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET7839742.1"
                     /translation="MRILAAIRSIPKGRVTSYGLIARLAGMPNGARQVVRALHTSSGS
                     QDLPWFRLLRKDGSIALPQGEGFELQKALLEAEGVKVGKGGKVDLASFGWDGTAPNLE
                     EAPNPDGPGRGPRRSGSHGGKAAPRTRRGAGP"
     gene            583..1503
                     /gene="fabD"
                     /locus_tag="VFL04_08280"
     CDS             583..1503
                     /gene="fabD"
                     /locus_tag="VFL04_08280"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET7839743.1"
                     /translation="MKTCFLFPGQGAQFPGMGRDFYETSQLVRELFELASDSAKLDLK
                     RLIFEGSEEELKQTRNTQLSMALVEAASALCAREHGIMPSAAAGFSVGEWPALAEAGV
                     ISNADMFRLVAERGRLMDEAGKRSGGSTMSAVLFLAPEAIEAAIAAAGLKQVWVANYN
                     SPTQSVISGTEADVAAAEEALKAAGAKRVVRLKVSGAFHSPIMAYARDGFAEAVAGVT
                     FSDPKIELFSNVSGKRITSGAEAKKFAVEQIVSPVRWIEEEKSILESGISLCLETGPG
                     TVLTGLWKAVAGAVPCQACGTLDQIAAISL"
     gene            1528..2277
                     /gene="fabG"
                     /locus_tag="VFL04_08285"
     CDS             1528..2277
                     /gene="fabG"
                     /locus_tag="VFL04_08285"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET7839744.1"
                     /translation="MEALLKDKVCVITGGSKGIGRSIVDLFARQGGKVYYLSRSEADD
                     PASLAAAASSGGGFVKWISCDVCDEGQVDAAVETVIKEAGAIDALVNNAGVTRDGLVF
                     RMSLADWNTVITTNLTSAFLVSRVAARHMIKRRSGSIVNVSSVVGIMGNGGQTNYAAS
                     KAGVIGFTKSLAREVSARGVRVNAIAPGFIETAMTEKIPAEAKEKMKATIPLGRTGRP
                     EEIAAAALYLCSELSSYVTGEVLKVDGGMGM"
     gene            2280..3518
                     /gene="fabF"
                     /locus_tag="VFL04_08290"
     CDS             2280..3518
                     /gene="fabF"
                     /locus_tag="VFL04_08290"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET7839745.1"
                     /translation="MERRRVAVTGLGVVSPVGNNVPDFWEAVKAGRSGIGPITSIKSD
                     DLAARIAGEVRGFDPTLRIEAKEARKMDRFSQFAVVSALEALEDAGLRPEDLDPERTG
                     VCIGTGQGGSASIEEGIARLSERGPSRVPPMTVAKALANFGAANVAIYAGATGPCQCI
                     VTACAAGTDAIGQGLQWVRHGLADVVLAGGAEASIIRVCVASFSMIQALSTRNEEPEA
                     ASRPFDADRDGFVIAEGAGVLVLEDWDRAVARGAKIYCELAGFANTCDAHHLTAPDPE
                     GMGAARAMKLAMRDACIEPEGVDYVSAHGTSTPMNDPIETKAIKHAFGEHARRLKVSS
                     LKSMIGHCIGAAGAVESVAVALAIRDGFVPATINLRKADPECDLDYVPNVGVSMPIRA
                     AVKNSMGFGGQNAVIVFKHA"
     gene            3537..>3955
                     /locus_tag="VFL04_08295"
     CDS             3537..>3955
                     /locus_tag="VFL04_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HET7839746.1"
                     /translation="MTESMPAVRPGNPLPLGARLLAGGVDFSIFSRNAEAMTLCVYAH
                     STDGKPSFEYRLDPRLNKTGDIWHVHVPSLKAGALYLWRAEGPYLPHKGFRFNPHKAL
                     IDPYAKALSGDFVWDLPAARGFDPASPDKDLSFSAVD"
BASE COUNT          660 a         1405 c         1285 g          605 t
ORIGIN      
        1 ggccctgctc cccggcgagt acggggagcg gctttccctc catggctacc gctcctacgg
       61 ggtccacgcc caggcccgtc cggattagga gcctcctcca ggttcggagc cgtgccgtcc
      121 cagccgaagc tcgcgagatc caccttcccg cctttgccga ccttgacgcc ctcggcttcg
      181 aggagggcct tctggagctc gaagccctcg ccttgaggga gggcgatgga gccgtccttg
      241 cggaggaggc ggaaccaggg cagatcctgg ctgcccgaac tcgtatggag ggccctgacc
      301 acctgcctcg ccccgttcgg catgccggcg agcctcgcga tgaggccata gctggtcaca
      361 cgtcccttcg ggatgctgcg gatcgccgcg agtatcctca tcgtctgctc cgaggccact
      421 gtcaccccca tccgacctcg agtatagttc gaggcggcac gagcggtaag ccgaatccgg
      481 gcgctggtcc atctttacaa atatccgttt tttgtaaata ttggatccgg cgaaaaccgg
      541 ccgaaggaca ccttcgccga cagcgtcgcg aggaggcaat aaatgaagac atgtttcctg
      601 tttcccggcc aaggcgccca gttccccggc atgggcaggg atttctacga gaccagccag
      661 cttgtgcgcg agctcttcga gctcgcctcg gattcggcca agctcgacct caagcggctc
      721 atcttcgagg gcagcgaaga ggagctcaag cagacccgca acacccagct ctccatggcc
      781 ctggtcgagg cggcctcggc cctctgcgcg cgcgagcacg gcatcatgcc gagcgccgcg
      841 gcaggcttct cggtcggcga atggccggcc ctcgccgagg ccggggtcat ctccaacgcc
      901 gacatgttcc gcctcgtcgc cgagcgcggc aggctcatgg acgaggccgg gaagcgctcg
      961 ggcggctcca cgatgagcgc cgtcctcttc ctcgctcccg aggccatcga ggcggcgatc
     1021 gccgcggccg ggctcaagca ggtctgggtg gcgaactaca acagccccac ccagtccgtc
     1081 atctcgggca ccgaggccga cgtcgcggcc gccgaggagg ccctcaaggc ggcgggcgcc
     1141 aagcgggtcg tgaggctcaa ggtctccggg gccttccact ccccgatcat ggcctatgcc
     1201 cgcgacggct tcgcggaggc ggtcgccgga gtcaccttct ccgacccgaa gatcgagctc
     1261 ttctccaacg tgtcgggcaa gaggatcacg agcggggccg aggctaagaa gttcgccgtc
     1321 gagcagatcg tcagccccgt gcgctggatc gaggaggaga agtcgatcct cgagtccggc
     1381 atcagcctct gcctcgagac ggggccgggg acggtgctca cgggcctttg gaaggcggtc
     1441 gccggggccg tgccctgcca ggcctgcggc actctggacc agatcgcggc tatcagccta
     1501 taaaccagcg aaggcaggaa gcaccacatg gaagcactac ttaaggacaa ggtttgcgtc
     1561 atcacgggcg gatccaaggg gatcggccgc tccatcgtcg atctcttcgc gcgccagggc
     1621 gggaaggtct actacctctc gcgctccgag gccgatgatc cggcctccct cgccgcggcg
     1681 gcgtcttccg gcggcggctt cgtgaaatgg atctcctgcg acgtctgcga cgagggccag
     1741 gtggacgcgg ctgtcgagac ggtcatcaag gaggccgggg ccatcgacgc cctcgtgaac
     1801 aacgcggggg tcacccgcga tggcctcgtg ttccgcatga gcctcgccga ctggaacacc
     1861 gtcatcacca cgaacctcac gagcgccttc ctcgtctccc gcgtcgccgc ccgacacatg
     1921 atcaagcggc gctccgggag catcgtcaac gtctcgagcg tcgtgggcat catgggcaac
     1981 ggggggcaga ccaactacgc cgcctcgaag gccggagtca tcggcttcac caagagcctg
     2041 gccagggagg tctcggcccg tggcgtccgc gtcaacgcca tagcccccgg tttcatcgag
     2101 acagccatga ccgagaagat ccccgccgag gccaaggaga agatgaaggc caccattccc
     2161 ctcggccgga ccggccggcc cgaggagata gccgcggcgg ccctctacct ctgctccgag
     2221 ctctcgtcct acgtgacggg agaggtgctc aaggtcgacg gcggaatggg gatgtaagca
     2281 tggaaaggcg gagggtggcc gtcaccggcc tgggcgtcgt cagccccgtg ggcaacaacg
     2341 tgcccgactt ctgggaggcc gtgaaggccg gcaggagcgg gatcggaccg atcacctcga
     2401 tcaagtccga cgacctcgcg gcgaggatcg ccggcgaggt ccgcggcttc gaccccaccc
     2461 tccgcatcga ggccaaagag gcgaggaaga tggaccgctt ctcccagttc gcggtcgtct
     2521 ccgcccttga ggccctcgag gacgctggcc tcaggcccga ggacctcgat cccgagcgaa
     2581 cgggcgtctg catcgggacg ggccaggggg gctcggcctc gatcgaggag ggcatcgccc
     2641 gcctctccga gcgcggccca tcccgggtcc ccccgatgac cgtcgccaag gccctcgcca
     2701 acttcggggc cgccaacgtg gcgatctacg cgggggccac gggcccctgc cagtgcatcg
     2761 tgacagcctg cgccgcgggc accgacgcca tcggccaggg cctccagtgg gtccgccacg
     2821 gcctcgccga cgtcgtcctc gcgggcgggg ccgaggcgag catcatccgc gtctgcgtgg
     2881 ccagcttctc catgatccag gccctctcga cccgcaacga ggagcccgag gccgcaagcc
     2941 ggcccttcga cgccgaccgc gacggttttg tcatagccga gggggcgggg gtactcgtcc
     3001 tcgaggactg ggacagggcc gtggcccgcg gcgcgaagat ctactgcgag ctcgccggct
     3061 tcgcgaacac ctgcgacgcc caccacctga ccgcccccga ccccgagggg atgggcgcag
     3121 cccgggccat gaagctcgcg atgcgcgacg catgcatcga gcccgagggc gtcgactatg
     3181 tgagcgccca cggcacatcc acccccatga acgatcccat cgagacgaag gccatcaagc
     3241 acgccttcgg cgagcacgcc aggcgcctca aggtctcgtc cctcaagtcg atgatcggcc
     3301 actgcatcgg cgccgcgggg gcggtcgagt ccgtggcggt ggccctggcc atcagggacg
     3361 gcttcgtgcc agcgaccatc aacctccgga aggccgaccc cgagtgcgac ctggactacg
     3421 tgcccaacgt gggcgtctcc atgccgatca gggccgcggt caagaactcc atgggtttcg
     3481 gcggccagaa cgcggtgatc gttttcaagc acgcgtgaag gcagtataat cgccttgtga
     3541 cggaatccat gcccgcggtc cgccccggca atcccctgcc cctcggagcg cggctcctgg
     3601 cgggcggcgt cgatttctcg atcttctcgc ggaacgccga ggcaatgacg ctctgcgtgt
     3661 acgcgcactc caccgacggc aagccctcgt tcgaataccg cctggatccc cggctcaaca
     3721 agaccgggga catctggcac gtccacgtcc cctcgctgaa ggcgggcgcc ctctacctgt
     3781 ggcgggccga gggcccctat ctgccgcaca agggcttccg cttcaacccg cacaaggccc
     3841 tcatcgaccc ctacgccaaa gccctgtcgg gcgacttcgt ctgggacctg ccggcggccc
     3901 ggggcttcga tcccgcctcc cctgacaagg acctctcctt ctcggccgtc gacga
//
LOCUS       DASSQV010000472         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_730889, whole genome shotgun sequence.
ACCESSION   DASSQV010000472 DASSQV010000000
VERSION     DASSQV010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730889"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFL04_08300"
     CDS             complement(<1..613)
                     /locus_tag="VFL04_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839747.1"
                     /translation="MKKSPAGPEGQAADRLAELYLREEESGEILLNRGILFYVLPMLA
                     AVVLGILVFGGPTPNLFLDAASVLAALAYYALIRRLLGAGLYRPWFKYLTVAVNLSLC
                     TITLVAYSFASTWIHSMRTMVVSSYILAVALSGLYHEPRIPVFAGLLAALQYSVIVAW
                     GLLAEAIPMSPVETFFAPAFSWDGYLFYLFMFLATGWIASFSAR"
     gene            858..1169
                     /gene="clpS"
                     /locus_tag="VFL04_08305"
     CDS             858..1169
                     /gene="clpS"
                     /locus_tag="VFL04_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HET7839748.1"
                     /translation="MAEISDIKGGVKTHQEEKTKEPEEFRVILINDDFTTMEFVVSVL
                     MTVFHKPLAEATRIMLDVHKKGRGIVGVYSYDIAVTKINQVHLLAKQNGFPLKCTMEK
                     A"
     gene            1176..>2183
                     /locus_tag="VFL04_08310"
     CDS             1176..>2183
                     /locus_tag="VFL04_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7839749.1"
                     /translation="MKVSQEVQAIFNAAYNEAKLRNHEYLTPEHILYAALSFEQVRSI
                     LAACEADIEQLRRGMESYFEQKIPQVKNSEPVQTAGFQSVIERAVLQSQAAGKEEVEV
                     ADVLVSLYDEERNYSAYYLRKSGVKRLQLLEVISHGEGHDGDAFEEDEEEEEEEEEGH
                     EEREERKPRAAGRMNPLERFASDLTRSAREGKLEPVIGRVAEIERTIQVLCRRLKNNP
                     IHVGDGGVGKTAITEGLAQRIVSGEVPPRLRDYTIYALNMGSLLAGTKFRGDFEERVK
                     RVVEELLKKEKAILFIDEIHTIVGAGAVSGGSLDASNLLKPALTSGKLRCIGSTTYDE
                     YN"
BASE COUNT          450 a          667 c          731 g          335 t
ORIGIN      
        1 gccgggccga gaaggaggct atccagcccg tcgcgaggaa catgaagagg tagaacaggt
       61 agccgtccca gctgaaggcg ggagcgaaga aggtctcgac cggggacatg gggatggcct
      121 cggcgaggag gccccaggcc acgatgaccg agtactggag cgctgccagg aggccggcga
      181 agacggggat ccgcggctcg tggtagagcc cggacagggc gacggcgagg atgtaggagg
      241 agaccaccat cgtcctcatg ctgtggatcc aggtcgaggc gaaggagtag gccacgaggg
      301 tgatggtgca gaggctcagg ttcaccgcga cggtgaggta cttgaaccag gggcggtaga
      361 gccccgcccc aaggaggcgg cggatgaggg cgtagtaggc cagggcggcg aggaccgacg
      421 ccgcgtcgag gaagaggttc ggggtcggcc ccccgaagac caggatcccc aggaccacgg
      481 cggcgagcat gggcaggacg tagaacagga tgccgcggtt gaggaggatc tcgccgctct
      541 cctcctcgcg gaggtagagc tcggcgaggc gatcggcggc ctggccctcg gggccggctg
      601 ggcttttttt catgatggca ccttggctca agtatagaac attggctcca atacctccaa
      661 taccgaggtc agcggccccg cctcgggcct tgctctcttt tttgccccaa ttaggttgcc
      721 gaaaggcctt ttgagcgtat tattgtcctc catggcccgg cttgggctcg gtttccggcc
      781 ggagcggggc ccatgggcgg cgggggcggg cttgtttttg gcctgcctac tcggtatttt
      841 gaaggttagt aaatgccatg gcggaaattt cggacatcaa gggcggggta aagacccacc
      901 aggaggagaa gaccaaggaa cccgaggagt tccgggtcat cctcatcaac gatgacttca
      961 ccaccatgga gttcgtcgtc tcggttctca tgacggtttt ccacaagccc ttggccgagg
     1021 cgacgcgcat catgctcgac gtccacaaga agggcagggg aatagtaggg gtctacagtt
     1081 acgacattgc cgtgacgaag atcaatcagg tgcacctgct ggcaaagcag aacggctttc
     1141 cgctcaagtg caccatggag aaggcatagg aacacatgaa ggtcagccag gaagtgcagg
     1201 caatattcaa cgccgcttac aacgaggcca agctccggaa ccacgagtat ctcactccgg
     1261 agcacatcct ctacgcggcc ctctcgttcg agcaggtgcg ctccatactc gccgcctgcg
     1321 aggctgacat cgagcagctc aggcggggca tggagtccta tttcgagcag aagatccccc
     1381 aggtcaagaa ctccgagccc gtgcagaccg ccggcttcca gagcgtcata gagagggcgg
     1441 tcctccagtc ccaggctgcg ggcaaggagg aggtggaggt ggccgacgtc ctcgtctccc
     1501 tctacgacga ggagcgcaac tactccgcct actacctccg caaatccggg gtcaagcggc
     1561 tccagctcct cgaggtcatc tcccacggcg agggccacga cggcgacgcc ttcgaggagg
     1621 acgaggagga ggaagaggag gaggaggagg gccacgagga gcgcgaggag cgcaagccca
     1681 gggccgccgg gcgcatgaac cccctcgagc gcttcgcctc cgacctcacg aggtcggccc
     1741 gcgagggcaa gctcgagccc gtgatcggcc gcgtggccga gatcgagcgc accatccagg
     1801 tcctctgcag aaggctcaag aacaacccca tccacgtcgg cgacggcggc gtcggcaaga
     1861 cggccatcac cgagggcctg gcccagcgca tcgtctcggg cgaggtcccg ccccggctgc
     1921 gcgactacac gatctacgcc ctcaacatgg gctcactcct cgcgggcacc aaattccgcg
     1981 gcgacttcga ggagagggtc aagcgcgtcg tcgaggagct cctcaagaag gagaaggcga
     2041 tcctcttcat cgacgagatc cacaccatcg tgggcgccgg agccgtgtcc ggcggctcgc
     2101 tcgacgcctc caacctcctc aagcccgccc tcacctcggg caagctgcgc tgcatcggct
     2161 cgaccaccta tgacgagtac aac
//
LOCUS       DASSQV010000473         4816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_234900, whole genome shotgun sequence.
ACCESSION   DASSQV010000473 DASSQV010000000
VERSION     DASSQV010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234900"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1951)
                     /locus_tag="VFL04_08315"
     CDS             complement(50..1951)
                     /locus_tag="VFL04_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7839750.1"
                     /translation="MNRATRSKAAARLRPAALAALAAFALMACASSPKPSIKDATPYS
                     DLKLQQLKDLAQADPARAVEAGVELMAATPGPGQGPSTSDVRSVAASAIGRLETAYRT
                     ALDGQDWRSARSLWLSLRAICGDGRLSDLVSGDPILGAIAKEETREPEFLAKDAEAFY
                     AKGLVTAALIQYHAALDAGRSRSKDFAAEELKRWTDRALAARDRVSLARFSQAFKALG
                     QSLPQAAIDLLAAKDQLSSMRKGVVTLNIDKGIRIEQGMGLPDRVLGSGFYIDAGGYL
                     ITNYHVIESEVDPTYKGYSKLTVRPADSPELRLPAKVVGWDRLLDIALVKVTATPEYV
                     FSLGDGTDLTPGQRIYAIGSPAGLENTITSGIVSAVGRRILQTGETVQVDAALNPGNS
                     GGPLVNEDGIASGVVFAGLPQFQNLNFAIPASWVSRIVPELFRGGELKRAWLGLALAE
                     AQDPSTEVKPGIEVLYRHPQMNSGIERGDRVISVDGAAFSRVPPAQGVMLGLSPGGLA
                     LVEVAKEGSTRKLLRYLGERPFIPLEAAAASDRHDRVFPALFGMAVSQIPGNLLEPEA
                     YMISKVWPGTVADEAGLSENDPFVLRRFMIDSDQHAAIIQIDVKKRKAGFLESIIQIP
                     APLDIPDFI"
     gene            complement(1966..3939)
                     /locus_tag="VFL04_08320"
     CDS             complement(1966..3939)
                     /locus_tag="VFL04_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839751.1"
                     /translation="MGRGALAVIVILAPILCLIPLAGVSAQAEPPSLARSWFEEARLL
                     AGAGDWAGAMGLLAEGIALDPMDSDSRYLASLASMKTARPGAEALAQVEAAIASGRFL
                     AYSIDDARLLRAELLLRSRRWQEALSSLGQGPGASTSSEYAWMRLRALLGLGDTAGFE
                     ALMASALRRFPDEPRFPRLFLERDVGGIPSDAARARGSTILARLPTYAEVDPELRALA
                     APLMPGQDARRDAVLAFRSMGGRSAAASLAALEYGIIDATGAAAELASGAYPLRLRDI
                     DRAYDLAGSEAGRAAVRAALASFDGTVGADRDGDGIDEEWTRLEKGRPVEWRRDPDQD
                     GSTILRVAFAAGLPSAFELIHGGTVIKGSFSSYPACSSLSFTASAPSDPRTPADQGVM
                     PGAGPRSETGGRSLTRTYLFGPEGFAYMPLIMPGPDAAGFLPAPGPDGPPTERAASAF
                     ALALREAVSGTGTETLTRLDRGLPQERDSYRDGRLFARLSYDKGRPTTERVDTDGDGV
                     FETERSYVWDGAEYYVAWVSVDTNGDGRFDYREQTRFPFRKEWDYGGKSGIDAVQFQQ
                     ADGSIRREFSSRLDGELDEVIVVSGGHVSTIIRDGKRPELIPDTNPGLRWIGTKPFDL
                     GSNLPGSEGVYTYMGVRYRIVKTGDGSFAEIIP"
     gene            complement(3942..>4816)
                     /locus_tag="VFL04_08325"
     CDS             complement(3942..>4816)
                     /locus_tag="VFL04_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HET7839752.1"
                     /translation="GRFDRQVVVAMPDVQERQDILAIHMQKIPVDEDVDVTRLARATP
                     GTSGADLANLVNEAALFAIRQNRVNVEMKDFEEARDKILMGVARTSLIITDEEKRATA
                     VHEAGHALLHYHLKYSDPLHKVTVVPRGRALGLALSIPEKDVYSRAKSWLEDRIAIMY
                     GGYAAEMLIYGETTTGTAQDLRQATDLARKMVCEWGMTDSLGPMAYGQEEEPIFIGKE
                     IAQHKDYSEDTARKIDAAIKDILQASLARVTGLLTEHRDQLEKLADELVSRETLDDND
                     VRALIGLPPRAGKA"
BASE COUNT          698 a         1572 c         1709 g          837 t
ORIGIN      
        1 gcccccgcct gcctcatcct cgtttctcgg ggcaggcgct ttcgtccccc tagatgaagt
       61 ccggaatgtc caagggggcg gggatctgga tgatgctctc cagaaagccc gccttccgct
      121 tcttcacgtc gatctggatg atggccgcat gctggtccga gtcgatcatg aaacgtctga
      181 ggacgaaggg gtcgttctcg gagaggccag cctcgtcagc gaccgtgccc ggccagacct
      241 ttgagatcat gtaggcctcg ggctcgagga ggttgccggg gatctggctc actgccatcc
      301 cgaacagggc ggggaagacc ctgtcgtgac ggtcgctcgc ggcagcggcc tcgagcggga
      361 tgaagggccg ctctccgagg tagcggagca gcttgcgcgt cgagccctcc ttggccactt
      421 caacgagggc gagtcccccg gggctcaggc cgagcatgac cccctgggcg ggcggcaccc
      481 tgctgaaggc cgcgccgtcg acgctgatga cacgatcccc gcgctcgatg cccgagttca
      541 tctggggatg gcgatagagt acctcgatgc ctggcttgac ctcggtgctc ggatcctggg
      601 cctcggcgag ggcaaggcca agccatgccc gcttcagctc acctcccctg aacagctcgg
      661 gcacgatccg ggacacccag ctggcaggta tggcgaaatt gaggttctgg aactggggca
      721 ggccggcgaa gaccacgccc gaggcgatgc cgtcctcgtt gacgaggggt ccgcccgagt
      781 tgccggggtt gagggcggcg tccacctgga cagtctcgcc ggtctgcagg atgcgccttc
      841 cgacggcgct cacgatcccc gaggtgatcg tgttctcgag cccggcgggg gaaccgatgg
      901 cgtagatcct ctggcccggc gtcaggtcgg tgccgtctcc aagcgagaac acgtactccg
      961 gggtggccgt gaccttgacg agggcgatgt cgaggagcct gtcccagccc accaccttcg
     1021 cgggcaggcg gagctcgggg gaatcggccg ggcggacggt gagtttcgag tagcccttgt
     1081 acgtgggatc gacctcgctc tctatcacgt ggtagttcgt gatgaggtag cctccggcgt
     1141 cgatgtagaa gcccgagccc agcacccggt cgggcaggcc catgccctgc tcgatcctta
     1201 tgcccttgtc gatgttgagg gtcacgacgc cctttcgcat cgaggagagc tgatccttcg
     1261 ccgccaggag gtcgatggcc gcctggggca ggctctggcc tagggccttg aaggcctgcg
     1321 agaagcgagc gaggctgacc ctgtccctgg cggccaatgc cctgtccgtc cagcgcttca
     1381 gctcctcggc ggcgaagtcc ttcgacctgc tccttcccgc gtcgagggcg gcgtggtact
     1441 ggatgagcgc tgcggtgaca aggcccttcg cgtagaaggc ctcggcgtcc ttggcgagga
     1501 actcgggctc cctcgtttcc tccttcgcta tggccccgag gatggggtcg cccgagacga
     1561 ggtcggacag gcgtccgtcc ccgcagatcg cgcgcaggct cagccacagc gagcgggccg
     1621 agcgccagtc ctggccgtcg agggccgtcc tgtaggcggt ctcgagcctg cctatcgccg
     1681 aggccgcgac ggagcggaca tcgctggtcg agggaccctg accggggccg ggcgtggcgg
     1741 ccatgagctc gaccccggcc tccacggcgc gggcgggatc ggcctgggcc aggtccttga
     1801 gctgctggag cttgaggtcc gagtagggcg tcgcatcctt gatcgatggc tttgggctcg
     1861 aggcgcatgc catgagggcg aacgcagcca gcgccgccaa ggcggcgggc ctgaggcggg
     1921 cggccgcctt gctccgggtc gctcggttca tgttgccacc gcctctcaag ggatgatttc
     1981 cgcgaagctg ccgtctccgg tcttcacgat ccggtatctg acacccatgt acgtgtaaac
     2041 cccttccgag ccaggcaggt tacttcccag atcgaagggc ttggtcccga tccacctgag
     2101 gcccggattc gtgtcgggga tgagctcggg ccgcttcccg tcgcggatga tcgtgctcac
     2161 atggccgccc gagaccacaa tgacctcgtc gagctctccg tccagcctcg atgagaactc
     2221 cctgcggatt gagccgtcgg cctgctggaa ctgcacggcg tcgatgccgc tcttcccgcc
     2281 gtagtcccat tcctttcgga aggggaaacg ggtctgctcg cggtagtcga agcggccgtc
     2341 gccgttggtg tccacgctga cccaggcgac atagtattcg gcgccgtccc acacatagct
     2401 gcgctcggtc tcgaagaccc cgtccccgtc agtgtcgacg cgctcggtag tgggtctgcc
     2461 tttgtcatag gagagtctgg cgaagagcct tccgtcgcgg tagctgtccc tctcttgggg
     2521 aaggccgcga tcaagacggg tgagggtctc ggtccccgtt ccgctgacgg cttcgcgcag
     2581 ggccagggcg aaggccgagg ccgccctttc ggtgggtggg ccgtcggggc cgggggcagg
     2641 caggaagcct gccgcgtcgg ggcccggcat gatgaggggc atgtaggcga agccttcggg
     2701 cccaaagaga taggtcctgg tgaggctgcg ccccccggtc tcggaccggg gccccgcacc
     2761 gggcatgacc ccctggtcgg ccggggtcct cgggtcagag ggggccgacg cggtgaagct
     2821 caggctggag caggctggat agctcgagaa gctgcctttg atgacggtgc cgccgtggat
     2881 cagctcgaag gccgatggaa ggccggcggc gaaggccacg cgcaggatcg tggagccgtc
     2941 ctggtccggg tcccttcgcc attccacggg tctgcccttc tccagcctcg tccattcctc
     3001 gtcgatcccg tcgccgtcgc ggtcggcccc gactgtcccg tcgaagctgg ctaaggctgc
     3061 cctgaccgcg gccctgcccg cttcgctgcc tgccaggtcg taggccctat cgatgtcgcg
     3121 gaggcgcagg ggataggcgc ccgaggccaa ttcggccgca gctcccgttg cgtcgatgat
     3181 gccgtattcc agagcggcaa ggctcgcggc cgcgctcctg ccgcccatcg atctgaaggc
     3241 gaggacggcg tccctccggg catcctgccc cggcatgagg ggagccgcga gggcgcgcag
     3301 ctccgggtcg acctcggcgt aggtcggcag cctcgcgagt atggtgctcc ctctcgccct
     3361 ggccgcgtcc gagggtatcc cgccgacatc ccgctcgagg aaaagccgcg gaaagcgcgg
     3421 ctcgtcgggg aagcgccgca gggccgaggc catcagggcc tcgaagcccg cagtgtcgcc
     3481 gagccccagg agggctctca ggcgcatcca cgcgtactcc gaagacgtcg aggccccggg
     3541 accctggccc aggctggaga gggcctcctg ccagcgcctg gagcgcagga ggagctcggc
     3601 cctgaggagc ctggcatcgt cgatgctgta ggcgaggaaa cggcccgagg cgatggccgc
     3661 ctccacttgg gcgagggctt cggcgcccgg cctcgccgtc ttcatcgagg cgagggaggc
     3721 gaggtagcgg gaatccgaat ccatggggtc gagggcgatg ccctcggcga ggagccccat
     3781 cgcccccgcc cagtctccag cccccgcgag gagccgggct tcctcgaacc aggaacgggc
     3841 gagggaaggg ggctcggctt gggcggagac cccagcgagg ggaatgaggc agaggatcgg
     3901 ggctagaatg acgatgactg ccagtgcgcc ccgacccatg ctcaggcctt tcccgctctc
     3961 ggcggcaggc cgatcagggc gcgcacgtcg ttgtcgtcga gggtctccct cgagacgagc
     4021 tcgtcggcga gcttctcgag ctggtcgcgg tgctcggtga ggaggccggt cacccgagcg
     4081 agcgaggcct gcaggatgtc cttgatggcc gcgtcgatct tcctcgccgt gtcctcggag
     4141 tagtccttgt gctgggctat ctccttgccg atgaagatgg gctcctcctc ctggccgtag
     4201 gccatcgggc cgagggagtc cgtcatcccc cattcgcaga ccatcttcct cgcgaggtcg
     4261 gtagcctgtc ggaggtcctg ggccgtgccc gtcgtcgtct cgccgtagat gagcatctct
     4321 gcggcgtagc cgccgtacat gatggcgatg cggtcctcga gccaggattt ggcccgggag
     4381 tagacgtcct tctcggggat cgagagggcg aggccgaggg ccctcccccg cgggacgacg
     4441 gtgaccttgt ggaggggatc ggagtatttg aggtggtagt gcaggagggc gtggcccgcc
     4501 tcatggacgg cggtcgcgcg cttctcctcg tcggtaatga tgaggctggt gcgcgcgacc
     4561 cccatgagga tcttgtcccg cgcctcctcg aagtccttca tctcgacatt gaccctgttc
     4621 tgcctgatgg cgaagagggc ggcctcgttg acgaggttcg cgaggtcggc ccccgaggtg
     4681 ccgggcgtgg cccgggcgag gcgggtgacg tcgacgtcct cgtccacggg gatcttctgc
     4741 atgtggatgg cgaggatgtc ctggcgctcc tggacatcgg gcatagcgac cacgacctgg
     4801 cggtcgaagc gtcccg
//
LOCUS       DASSQV010000474         4867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_235615, whole genome shotgun sequence.
ACCESSION   DASSQV010000474 DASSQV010000000
VERSION     DASSQV010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235615"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFL04_08330"
     CDS             complement(<1..402)
                     /locus_tag="VFL04_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7839753.1"
                     /translation="MRELLDMVPSVLMMVAPILIAAMGGMISEKAGIVNIALEGLMGI
                     GAFTAATTHVLLGGSAQITVWVAFGTALAAGAAISLIHAYASVSLNADQVVSGTGINL
                     LAMGSTVFLCQIIFHQERTEPYKLGMLPGPGG"
     gene            complement(395..1537)
                     /locus_tag="VFL04_08335"
     CDS             complement(395..1537)
                     /locus_tag="VFL04_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7839754.1"
                     /translation="MRAAKARSGGSRLLDAILAAPRTVSIFVVVLGFVCGTILILAVG
                     RNPGGMYAALVQTLTGFDLTRGTWNSRYIGEWLTASMPLILCGMSMGFAARTGLFNIG
                     AEGQYIVGLSIAQAVALFGPQVSGLHWILAVLAAGIGGAAWGGIVGWLKAKYAVSEVV
                     ATIMLNYIAFFLSRYFTRLIPDANTYQTPAFPQTARIPSGIFDSLTNGSRLNAGIWVT
                     LLCVLAYWFIIERTRLGFSLRATGLNKDAARYGGIRVTRSIVVSMGIAGSFAGLAGAI
                     VALGSFSYGRVLGGYDNYGFNGIAVALVGNSTGGGTLVSGLLFGMLASAQPLMQSRQI
                     PKEVTWIISGLVVVFISLRTGFQMLIDWRAKRMTARDEAANGGQDA"
     gene            complement(1534..3072)
                     /locus_tag="VFL04_08340"
     CDS             complement(1534..3072)
                     /locus_tag="VFL04_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7839755.1"
                     /translation="MDHYAIEMLEVTKVFPGVVANDKVTIRVLDNEIHAILGENGAGK
                     STLMSILFGLYDPDSGRILIRGKEVRIRDPNAATALGIGMVHQHFKLVHNYTVTENIV
                     LGMEPRNKAGNLDLSGAERRVAELSERYGLAVDPKAKVETITVGMQQRVEILKILYRN
                     ADILIFDEPTAVLTPQEIDELIAIFRRLKAEGKTIILITHKLREIKEAAERCTVLRRG
                     RYIGTVSVDATSEEEMARMMVGREVKFHIEKAPPKPGEVVLRVEGLTVKNAKGFAAVS
                     NLSLEVRAGEILGIAGVDGNGQSELIQALTGLTLPDSGRIEFKGRDITRSSIRERIRA
                     GMGHVPEDRHKHGLVLGFRLDENLVLKTFDLPPFSDARGILDFAAMEKHGAELIAQYD
                     IRAGEGPATKAGSMSGGNQQKAIIAREIDLSPELLVVAQPTRGLDVGAIEHIHRRIIA
                     ERDKGRAVLLVSFELDEVMDLCDRIAAISKGEIVGIVEAEGADERAIGAMMAGIRSQA
                     GRTA"
     gene            complement(3208..4305)
                     /locus_tag="VFL04_08345"
     CDS             complement(3208..4305)
                     /locus_tag="VFL04_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7839756.1"
                     /translation="MKKILFALLAAAVVLFAAGCGGKQAAKSTEPTTRLLTDATGIDD
                     KSFNAAAWRGIVAFYGDTLDKTEKRGKLYDVVTAQTQDMYVPNLKQAADEKYDLIIVT
                     GFTWADALNEVAPKYPNQKFMIVDVDWVNQPNVMQVTFTEHEGSYLVGVAAALKAKAD
                     GIKNPKFGFIGGIPGPIITKFEMGYVQGILSVLPKAKIVDYYANDWGKPELAKAQAKN
                     WYDSGVYVIYSAAGGTGNGTIAQAKEYRTQGKNVWAIGVDSDQYADGIYAEGKSAVLT
                     SMLKRVETASKVALESVKDKTFKAQVVVLDTKSDGVGFSSANAELGQDIVAQVEAARK
                     DIIDGKVKVLATYAEAKAAGKVPAGLQASDK"
     gene            complement(4435..>4867)
                     /locus_tag="VFL04_08350"
     CDS             complement(4435..>4867)
                     /locus_tag="VFL04_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7839757.1"
                     /translation="TDWLKQYSDSDDETYVRSFLGRMLFSGDEALKPVRVLSGGEKVR
                     CVLSKLMLSGANCLILDEPTNHLDLEAITALNDGLIEFEGVLLFTSHDHEFVTSIANR
                     IVEICPGGVIDRYMSFDDYIRDKQVSALRDQYYHGHVIAEI"
BASE COUNT          765 a         1508 c         1703 g          891 t
ORIGIN      
        1 cccccccggc ccgggcagca tgccgagctt gtagggctcg gtccgctcct gatggaagat
       61 gatctggcag aggaagacgg tgctccccat cgcgaggagg tttatgcccg tgcccgagac
      121 gacctggtcg gcgttgagcg acaccgaggc gtaggcgtgg atgagggaga tggcggcccc
      181 cgcggcgagt gcggtcccga aggccaccca gaccgtgatc tgggccgagc cgccgagcag
      241 gacatgggtg gtcgcggcgg tgaaggcccc tatgcccatg aggccctcga gggcgatgtt
      301 cacgatgccc gccttctctg agatcatgcc ccccatggcc gcgatgagga tgggtgccac
      361 catcatgagc acggaaggga ccatgtcgag cagctcacgc atcctgtcct ccgttcgcgg
      421 cctcgtcgcg cgccgtcatg cgcttcgccc gccagtcgat gagcatctgg aagcccgtgc
      481 ggagggagat gaagaccacc acgaggcctg agatgatcca ggtgacctcc ttcgggatct
      541 gcctggactg catgaggggc tgggccgagg cgagcatgcc gaagagcagg cccgagacga
      601 gggtgccccc gcccgtgctg tttcccacga gggccacggc tatcccgttg aagccgtagt
      661 tgtcgtagcc gccgaggacc cgcccgtagc tgaaggagcc gagggccacg atcgcgccgg
      721 cgaggccggc gaagctgccc gcgatgccca tcgagaccac gatcgaccgc gtcacgcgga
      781 tgccgccgta cctcgccgcg tccttgttga ggcccgtcgc tcgcagcgag aagccgagcc
      841 ttgtgcgctc gatgatgaac cagtacgcga ggacgcagag cagggtcacc cagatgccgg
      901 cgttcagccg cgagccgttg gtgaggctgt cgaagatgcc ggaggggatg cgcgcggtct
      961 gggggaaggc cggggtctgg taggtgttgg cgtcggggat gaggcgggtg aagtagcgcg
     1021 agaggaagaa ggctatgtag ttgagcatga tcgtggccac gacctcggag accgcgtact
     1081 tggccttgag ccatcccacg atgccgcccc atgccgcgcc cccgatcccc gcggcgagga
     1141 cggcaaggat ccagtggagg cccgagacct gaggcccgaa gagggccacc gcctgggcga
     1201 tgctcagccc gacgatgtac tggccctcgg ccccgatgtt gaagaggccg gtgcgcgcgg
     1261 cgaagcccat ggacatgccg cagaggatca ggggcatgga ggccgtgagc cactcgccga
     1321 tgtagcgcga gttccaggtg ccgcgcgtga ggtcgaagcc cgtgagggtc tggaccaggg
     1381 ccgcgtacat cccgcccggg ttcctcccga cggcgaggat gaggatggtg ccgcagacga
     1441 agccgaggac cacgacgaag atcgagaccg tcctcggggc ggcgaggatc gcatcgagga
     1501 ggcgcgagcc cccgctcctt gccttggccg ccctcatgcc gtcctccccg cctgcgagcg
     1561 gatgccggcc atcatggcgc cgatggcgcg ctcatcggcg ccctcggcct cgactatgcc
     1621 cacgatctcc cccttggaga tggccgctat ccgatcgcag aggtccatga cctcgtcgag
     1681 ctcgaaggag accaggagga cggcccttcc cttgtcgcgc tcggcgatga tcctgcggtg
     1741 gatgtgctcg atggccccca cgtcgagccc gcgggtgggc tgggcgacga cgaggagctc
     1801 gggcgagagg tcgatctcgc gcgctatgat cgccttctgc tggttgcccc ccgacatcga
     1861 tcctgccttt gtggccgggc cctcgcccgc cctgatgtcg tactgggcga tgagctcggc
     1921 cccgtgtttc tccatggcgg cgaagtccag gatgccgcgg gcatcggaga agggggggag
     1981 atcgaaggtc ttgaggacca ggttctcgtc gaggcggaag ccgaggacca ggccgtgctt
     2041 gtgcctgtcc tcgggcacgt gccccatgcc ggctcggatc cgctcgcgta tgctggagcg
     2101 ggtgatgtcg cggcccttga actctatccg ccccgaatcg gggagggtaa ggcccgtgag
     2161 ggcctggatc agctcggact ggccgtttcc gtcgaccccg gctatcccaa ggatctcgcc
     2221 ggccctgacc tcgaggctca ggttgctgac ggcggcgaag cccttggcgt tcttgaccgt
     2281 gaggccctcg accctgagca ccacctctcc cggtttcggt ggggcctttt cgatgtggaa
     2341 cttcacctcg cggcccacca tcatcctcgc catctcctcc tcgctcgtgg cgtcgacgga
     2401 gaccgtgccg atgtacctgc cgcgcctgag cacggtgcag cgctcggccg cttccttgat
     2461 ctcgcggagc ttgtgcgtga tcaggatgat ggtcttcccc tcggccttga gccgcctgaa
     2521 gatggcgatc agctcgtcga tctcctgggg cgtgagcact gccgtcggct cgtcgaagat
     2581 gaggatgtcg gcgttgcggt agaggatctt caggatctcg acgcgctgct gcatgccgac
     2641 ggtgatggtc tccaccttgg ccttcgggtc gacggcgagg ccgtagcgct cggagagctc
     2701 ggccacgcgg cgctcggccc cgctcaggtc gaggttgccg gccttgttgc gcggctccat
     2761 gccgaggacg atgttctcgg tgaccgtgta attgtgcacg agcttgaagt gttggtgcac
     2821 catgccgatg ccgagggcgg tcgcggcgtt cgggtcgcgg atcctgacct ccttgcctcg
     2881 gatcaggatg cggcccgagt ccgggtcgta cagaccgaag aggatggaca tcagggtcga
     2941 cttgccggcc ccgttctcgc cgagtatcgc gtggatctcg ttgtcgagga cgcggatcgt
     3001 caccttgtcg ttcgcgacga ctccggggaa gaccttcgtg acctcaagca tctcgatcgc
     3061 atagtggtcc atggatcact tcttccgtgg agaaacggag agccggcggg gcggggccgg
     3121 ttgggaggac cccggacccg ccggctctcg atgattgcgc cggaccgcgg ccaaatgacc
     3181 gaggcccggc gaaggggctg cggactttta cttgtcgctc gcctggagac ctgcggggac
     3241 cttgccggcg gccttggcct cggcgtaggt cgcgaggacc ttgaccttgc cgtcgatgat
     3301 gtccttgcgg gcggcctcga cctgggccac gatgtcctgg ccgagctcgg cattggccga
     3361 ggagaagccg acgccgtcgc tcttggtgtc gaggaccacg acctgggcct tgaaggtctt
     3421 gtccttcacg gactcaagcg cgaccttgga ggcggtctcg accctcttga gcatggaggt
     3481 caggacggcg gacttgccct cggcgtagat gccgtcggca tactggtcgg agtcgacccc
     3541 gatcgcccac acgttcttgc cctgggtcct gtactccttg gcctgggcga tggtgccgtt
     3601 gcccgtgccg cccgctgcgg agtagatcac gtacacgccg gaatcgtacc agttcttggc
     3661 ctgggccttg gcgagctcgg gcttgcccca gtcgttcgcg tagtagtcca cgatcttggc
     3721 cttggggagg acggagagga tgccctggac atagcccatc tcgaacttcg tgatgatggg
     3781 gccggggatg ccgccgatga agccgaactt ggggttcttg atcccgtcgg ccttggcctt
     3841 gagggccgcg gcgacgccga cgaggtagga accctcgtgc tcggtgaagg tgacctgcat
     3901 gacgttcggc tggttgaccc agtccacgtc gacgatcatg aacttctggt tcgggtactt
     3961 gggggcgacc tcgttgagcg cgtcggccca ggtgaagccg gtcacgatga tgaggtcgta
     4021 cttctcgtcg gctgcctgct tcaggttcgg gacgtacatg tcctgggtct gggcggtgac
     4081 gacgtcgtag agcttgccgc gcttctcggt cttgtccagg gtgtcgccgt agaaggcgac
     4141 gatgccgcgc caggccgccg cgttgaagga cttgtcgtcg atgcccgtgg catcggtgag
     4201 gagcctcgtg gtgggctcgg tggacttggc cgcctgcttg ccgccgcagc ccgccgcgaa
     4261 caggacgact gccgctgcga ggagggcgaa aaggattttc ttcatgctga gtacctccgg
     4321 ggactagctg gcgccctctg agggcgcgta acgcgaatca taggttcgcc gatcgatacg
     4381 cgtcaaggcg ggccggggtt tagtgtttgc aggaagaatc caaaaaatct ccagtcaaat
     4441 ctcagctatg acatggccgt ggtagtactg gtcgcggagg gccgaaacct gcttgtccct
     4501 gatgtaatcg tcgaagctca tgtagcggtc gatcaccccg cccgggcaga tctccacgat
     4561 ccggttcgcg atcgaggtga cgaactcgtg gtcgtggctc gtgaaaagga ggacgccctc
     4621 gaactcgatg agcccgtcgt tgagggcggt gatggcctcg aggtcgaggt ggttcgtcgg
     4681 ctcgtcgagg atcaggcagt tggccccgga gagcatgagc tttgagagga cgcagcggac
     4741 cttctcgccg ccggagagga cccggacggg cttcagggcc tcgtcgcccg aaaacagcat
     4801 cctcccgagg aaggagcgga cataggtctc gtcgtcggaa tcgctgtact gcttgagcca
     4861 atcggtg
//
LOCUS       DASSQV010000475         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_235712, whole genome shotgun sequence.
ACCESSION   DASSQV010000475 DASSQV010000000
VERSION     DASSQV010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235712"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..484)
                     /locus_tag="VFL04_08355"
     CDS             complement(68..484)
                     /locus_tag="VFL04_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET7839758.1"
                     /translation="MRSHTEELWFQTSKRFEFIDITERVQKALAASGIAEGLCLVNAM
                     HITSSVFINDAESGLHADFQEWLEKMAPHEPVSRYRHNVGEDNADAHIKRQIFGREVV
                     VAVTKGELHFGPWERIFYGEFDGRRRKRVLVKIIGE"
     gene            529..2796
                     /locus_tag="VFL04_08360"
     CDS             529..2796
                     /locus_tag="VFL04_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7839759.1"
                     /translation="MKAVLIQPPFTQLNAPYPAVHYLEAFLRAKGIEAQAFDHSIELF
                     RGLFSRAGLSRVFADARAALAARGPEPDPAAAREIERYLSYEGLYLEWADPLLSFLSG
                     GDPALAHRLASAAELPLGMRASSFLEGHGRRIDAGEARGLATRMLEDLGDFLSYTLDP
                     EFATVRYGERIASSKDDFGEVRAALDASYLLKAHYRGFLDAFWADQGAKADLVLISIP
                     FPGCLLGALACARETRSLLGDKALILFGGGYVSTELRDLMDPGIFDFCDYLCFDAGYG
                     SLASILESGPGRGSDAGAGRGLFRTMYRRGNEIHASGFGPEGSSPDQSSRRLIPCPDE
                     EGYAREEKEALARVFPDYAGTDFGLYLRVVDSDNPMHRLWSDSPWLKFHLAHGCYWRR
                     CAFCDTELEYVADFVPADTVALMEAARLASAGSGLSGIHFVDEAMPMAKLLAFALANR
                     AAAARGEKPFHFWGNVRFDSAWNPDRCELLAASGLVAVSGGIEIATDCGLELTDKGFD
                     LAGLVASLVALRRSGILVHAYLIYGFPGQDEAEIVDSAEVCRQLFASGLIDSAFWHRF
                     VLTRHSRMYAEWREGGRPSLKPLDRPHRFADNDLGFEGESGYDRFEQPLSASLAAWMA
                     GAELDRRADRWFPVRGRSKPPRVSATLIEDLIAEAGKRQDEARTSAEGRVFWLGGAAL
                     ADGQNPGRVTWAYRGELEELRLGAGAALRVAEAISRLRAEDAGLQLEAFLEASGIRRG
                     DEAFAALRASGLAVI"
     gene            complement(2824..>3449)
                     /locus_tag="VFL04_08365"
     CDS             complement(2824..>3449)
                     /locus_tag="VFL04_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET7839760.1"
                     /translation="SVLAGPGARTRELAFSPDGSTIAAGYDNAELWLWDRASEKPRAK
                     LAGGRRYVLALCYSPDGTTLAEGGVDGQLRLRDARTGETLREMRIASPIGDVDFSPDG
                     GILGSASDDNRLRLWDLNTGELICVLEGHRGFVNGLAFSPDGSLIASGSHDGRVGLWD
                     VREGSLMRMLEGHGDAVLRVAFSPDGRRLATISWDGTILVWGLAAAP"
BASE COUNT          513 a         1240 c         1104 g          592 t
ORIGIN      
        1 ctcaacaaaa ccactcatcc gaaaccgctc ggccccgacg cttccccgcg gatatccccg
       61 gattcctcta ctccccgatg atcttcacca gcacccgctt gcgccgccgc ccgtcgaact
      121 cgccatagaa gatcctctcc cagggcccga agtggagctc tcccttggtg accgcaacca
      181 cgacctccct tccgaagatc tggcgcttga tgtgggcgtc ggcgttgtcc tcgcccacgt
      241 tgtggcggta gcgcgaaacc ggctcgtgcg gagccatctt ctccagccac tcctggaagt
      301 cggcgtggag gcccgactcg gcgtcgttga tgaagacgct ggaggtgatg tgcatcgcgt
      361 tgaccagaca gagtccctcg gctatcccgg aagcggccag ggccttctgg acccgttcgg
      421 taatgtcgat gaactcgaag cgcttgctcg tctggaacca gagctcttcg gtatgggatc
      481 tcatgcctca agtatacgcg ctccgagcat cggggctata gtcgggatat gaaggccgtc
      541 ctgatacagc ctccctttac ccagctcaac gccccctacc ccgcggtcca ctacctcgag
      601 gcattcctgc gggccaaggg gatcgaggcc caggccttcg accactcgat cgagctcttc
      661 cgcggcctat tctcccgggc cgggctctcg cgggtattcg ccgatgccag ggccgccctc
      721 gcggcgaggg gccccgagcc cgacccggcc gcggcccggg agatcgagcg ctacctctcc
      781 tatgaggggc tctatctcga gtgggccgat cccctcctct ccttcctgag cgggggcgat
      841 cctgccctcg cgcaccgcct ggcctcggcc gccgagctcc ccctcggcat gcgggcctcc
      901 tcattcctcg agggccatgg ccggaggatc gacgcaggag aggcccgagg cctggccacc
      961 cgcatgctcg aggacctcgg cgatttcctc tcctataccc tcgatcccga gttcgcgacg
     1021 gtgcgctacg gcgagaggat cgcctcgagc aaggacgact tcggcgaggt ccgcgccgcc
     1081 ctcgacgcat cctacctcct caaagcgcat taccgcggct tcctggacgc cttctgggca
     1141 gaccaggggg caaaggccga cctcgtcctc atcagcatac cctttcccgg ctgcctcctc
     1201 ggcgccctcg cctgcgcacg ggagacccgt tccctgctcg gagacaaggc gctgatcctc
     1261 ttcggcgggg gatatgtctc gaccgaactc cgcgacctca tggatcccgg catcttcgat
     1321 ttctgcgact acctctgttt cgacgcgggc tacggcagcc tcgcctcgat cctggaatcg
     1381 ggcccgggcc gtggttccga tgccggggcc ggcaggggcc tgtttcggac catgtatcgt
     1441 cgcgggaacg agatccatgc ctcaggtttc ggccccgagg gatcgagccc ggatcagagc
     1501 tcgcgccggc tcatcccctg cccggacgag gagggctacg cccgcgagga aaaggaggcc
     1561 ctggccaggg tcttccccga ctatgccggg actgatttcg ggctctacct cagggtcgtc
     1621 gattcggaca atccgatgca caggctctgg tcggactcgc cctggctcaa gttccacctg
     1681 gcccacggct gctattggcg ccgctgcgcc ttctgcgaca ccgagctcga gtacgtggct
     1741 gatttcgtcc ccgctgacac tgtagccctc atggaggcag cccggctcgc ctccgcgggc
     1801 agcggcctca gcggcatcca cttcgtcgac gaggccatgc ccatggccaa gctcctggcc
     1861 ttcgccctcg ccaaccgcgc ggcggcggcc aggggcgaga agcccttcca cttctggggc
     1921 aacgtgcgtt tcgattctgc ctggaaccct gaccgctgcg agctccttgc cgcatcgggt
     1981 ctggtggccg tgtccggggg catcgagatc gccacggact gcggcctcga gctgactgat
     2041 aagggcttcg acctcgccgg cctcgtcgcc agccttgtag ccctccgcag gagcggcatc
     2101 ctcgtgcacg cctacctcat ctacggcttt cccggacagg atgaggccga gatcgtcgat
     2161 tcggccgagg tctgcagaca gctcttcgcc tcgggcctca tcgactcggc cttctggcac
     2221 cgcttcgtcc tgacccggca ttcccggatg tacgccgaat ggagggaggg cggacggccg
     2281 tccctcaagc ccttggacag gccccaccgc ttcgccgaca acgacctcgg cttcgagggc
     2341 gagtccggct atgaccgctt cgagcagccc ctctcggcct cgctcgcggc gtggatggcg
     2401 ggggcggagc tcgaccggcg cgccgaccgc tggttcccgg tcaggggcag gtccaagccg
     2461 ccacgggtca gcgccaccct catcgaggac ctgatagccg aggctggaaa gaggcaggac
     2521 gaggcgagga ccagcgcgga gggccgcgtc ttctggctcg gcggcgccgc gctcgcggac
     2581 ggccagaatc ctggccgcgt tacctgggcc tatcgcggcg agctcgagga gctgcggctt
     2641 ggagccgggg ccgcgctccg cgtggccgag gcgatctccc gccttcgggc cgaggatgct
     2701 ggcctccagc tggaagcctt cctcgaggcc tcgggaatca ggcggggcga cgaagccttc
     2761 gccgccctcc gcgcctcggg ccttgcggtg atctagggaa gaccgacgct gtggacccgg
     2821 cccctagggc gccgcggcca atccccagac aaggatggtc ccgtcccagc tgatcgtcgc
     2881 aaggcgtcgg ccgtcggggc tgaaggccac gcgcagcacg gcgtcaccgt ggccctcgag
     2941 catgcgcatg aggctgccct cacggacgtc ccagaggccg acccggccgt cgtggctgcc
     3001 cgaggcgatg aggctcccgt cggggctgaa ggccaggccg ttcacgaagc ccctgtggcc
     3061 ctcgaggaca catatgagct cgccggtatt cagatcccaa agacggagac ggttgtcgtc
     3121 gctggccgaa ccgaggatcc cgccgtcggg gctgaaatcg acatcgccga ttggggaggc
     3181 tatcctcatc tcgcgcaggg tttcgcccgt cctcgcgtcg cggagcctca gctggccgtc
     3241 gaccccaccc tcggcgaggg tcgtgccgtc ggggctgtag cagagggcga ggacatagcg
     3301 cctacccccg gcgagtttcg cccgcggctt ttccgacgcc cggtcccaaa gccagagttc
     3361 ggcgttgtcg tatccggccg cgatggtcga gccgtcgggg ctgaaggcga gctcccgggt
     3421 cctggccccg gggccagcga ggaccgaca
//
LOCUS       DASSQV010000476         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1974935, whole genome shotgun sequence.
ACCESSION   DASSQV010000476 DASSQV010000000
VERSION     DASSQV010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974935"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFL04_08370"
     CDS             complement(<1..222)
                     /locus_tag="VFL04_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839761.1"
                     /translation="MFTYSFKRIAQSVMTLLIVITFVFLLMRLLPIEGYFGDMYDKLS
                     PSQREAILNKMGLLDPWPVQLGKFYASLAR"
     gene            complement(398..2227)
                     /locus_tag="VFL04_08375"
     CDS             complement(398..2227)
                     /locus_tag="VFL04_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7839762.1"
                     /translation="MKKLFVPILVTLALLSVAQAGAADRKSGDYVEAYAGELTTLNYL
                     VSGSQNEHYMFANMLDGLVECDRFGILRPSLAKSWTVSPDGLVWTFSLRPGVKWLTWQ
                     GKEYDEVVAQDWVDAAKYIMTKTNASGTADVIYGVLKNGEQYWEGKVTDFSQVGVKAK
                     DKYTVEYTLSRPVPYFLSMLTYVCFLPANGRFMAEMGAKWGTDNKSVLYNGAYIMTSY
                     EPQSSRELLRNEKYWDRANVFIKRLTFKYNKESDTLAPELFLRNEVSFAPIPSTIIDT
                     WMKDSGRSAKIHPARMTSYSYFYALNFKPTFDAQYEPANWKVVVNNKAFRQSLFFGLD
                     RQAAMLTSEPYNPARRLQNTITPRGFANAGGKDYVDIGGLAAISKTESFNKAQALKFK
                     AQAMKELAGKATFPVKVLMPFRADMADWASRAQVVQQQLETLLGKDYLTVIPVAFPAT
                     GFLGATRRAGNYAIQECNWGPDYADPETYTDPFVTGSNYNWPELAEGYGTADRPKYES
                     LVDAAKAETANMAKRFELFAKAETYLINEAMVIPYARGISNMVAYEASKLEPFTCPFA
                     PFGLSELKFKGQIVLDKPLGTAEYQKLEAQWLKEKAAALTKVK"
     gene            2373..>2710
                     /locus_tag="VFL04_08380"
     CDS             2373..>2710
                     /locus_tag="VFL04_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839763.1"
                     /translation="MDFHKYHGLGNDYIVIDPEACPIGLDERAVRAICDRNYGVGSDG
                     ILHGPLAGGPELRIYNPDGSEAEKSGNGLRIFSWYLYESGRWSGEPRAIATRGGVVEA
                     AIADPGAGLV"
BASE COUNT          444 a          774 c          959 g          533 t
ORIGIN      
        1 gcgggcgagg gaggcgtaga acttcccgag ctggacgggc caggggtcga gcaggcccat
       61 cttgttgagg atggcctccc tctggctcgg ggagagcttg tcgtacatgt ccccgaagta
      121 gccctcgatg ggcaggagcc gcatgaggag gaagacgaag gtgatgacga tgaggagggt
      181 catgaccgac tgggcgatgc gcttgaacga gtatgtgaac atgccttgtg tggcccctac
      241 gggccatcct cccgcgctcg atgttctggc ttcagggaaa tggacgatgc gccgcggagc
      301 gacagaggac gcagagcccg gagggaggag tgaagcttct cctccaccgt ccctccacag
      361 ctctgcgcct ctgcgtctct gcggttctct ctctcctcta cttgaccttg gtgagggcgg
      421 ccgctttttc cttgagccac tgggcctcga gcttctggta ttcggcggtg ccgaggggct
      481 tatcgaggac gatctgtccc ttgaacttca gctcagacag cccgaagggg gcgaaggggc
      541 aggtgaaggg ctcgagcttg gaggcctcgt aggcgaccat gttcgaaatg ccgcgggcat
      601 aggggatgac catggcttcg ttgatgaggt aggtctcggc cttggcgaag agctcgaagc
      661 gcttggccat gttcgcggtc tcggccttcg cggcgtcgac gaggctctcg tacttcggcc
      721 tgtctgccgt gccgtagccc tcggcgagct cgggccagtt gtagttggag ccggtcacga
      781 aggggtcggt gtaggtctcg gggtcggcgt agtcggggcc ccagttgcac tcctggatgg
      841 cgtagttgcc cgcccgcctg gtcgcgccga ggaagccggt cgccgggaag gcaacgggga
      901 tcacggtgag atagtccttg ccgaggaggg tctcgagctg ctgctggacg acctgggccc
      961 ggctggccca gtcggccata tcggcgcgga agggcatcag gaccttgacg gggaaggtgg
     1021 cctttccggc aagctccttc atggcctggg ctttgaactt gagggcctgg gccttgttga
     1081 aggactcggt tttggagatc gcggcgaggc cgccgatgtc gacgtagtcc ttgcctccgg
     1141 cgttcgcgaa gccgcggggc gtgatcgtgt tctggagccg ccgggcggga ttgtagggct
     1201 cggaggtgag catcgcggcc tgccggtcga ggccgaagaa gagggactgg cggaaggcct
     1261 tgttgttgac aacgaccttc cagttggcgg gctcgtactg cgcgtcgaag gtaggcttga
     1321 agttgagggc gtagaagtag gagtaggagg tcatgcgggc cgggtgaatc ttggcgctcc
     1381 tgccggagtc cttcatccag gtgtcgatga tcgtgctcgg gatgggggcg aagctcacct
     1441 cgttccggag gaagagctcg ggggcaaggg tgtcggattc cttgttgtac ttgaaggtaa
     1501 gccgctttat gaagacattg gcgcggtccc agtacttctc gttgcgcagg agctcgcggg
     1561 agctctgggg ctcatagctc gtcatgatgt aggcgccgtt gtagaggacg gacttgttgt
     1621 cggtgcccca cttggccccc atctcggcca tgaacctgcc gttggcgggg aggaagcaga
     1681 cgtaggtgag catggagagg aaatagggca cgggccgcga cagggtgtac tccaccgtgt
     1741 acttgtcctt ggccttgacc ccgacctggg agaagtcggt caccttgcct tcccagtact
     1801 gctccccgtt cttgagcacg ccatagatca cgtcggcagt gcccgaggcg ttggtcttgg
     1861 tcatgatgta cttggcggcg tccacccagt cctgggcgac gacctcgtcg tactccttgc
     1921 cctgccaggt gagccacttc acgcccgggc ggaggctgaa ggtccacacg aggccgtcgg
     1981 gcgagacggt ccaggacttg gccaggctgg ggcggaggat gccgaagcgg tcgcactcga
     2041 cgaggccgtc cagcatgttg gcgaacatgt agtgctcgtt ctggcttccg ctcacgaggt
     2101 agttgagggt cgtgagctcg ccggcatagg cctcgacata gtcccccgac ttgcggtcgg
     2161 ccgcgcccgc ctgggcgacg gagagaaggg ccaaagtcac gaggatcggg acgaagagct
     2221 ttttcatcgc aatgcttccc cctgtttcta tggatgcggt gttttccgcc tgagggagga
     2281 ttatttcacc ccttccgggc gccgtcaacg cgaaaacttc cctctggtcc gtctatcgtt
     2341 ccggggtata atccgtgcgg agggatcgcc cattggactt ccacaaatac cacggcctgg
     2401 gcaacgacta catcgtgatc gaccccgaag cctgcccgat cgggctggac gagcgcgccg
     2461 tgcgcgcgat atgcgaccgc aactacgggg tgggctcgga cgggatcctc cacggtcccc
     2521 tcgccggcgg ccccgagctc aggatctaca accccgacgg gagcgaggcc gagaaaagcg
     2581 gcaatggcct caggatcttc tcctggtacc tgtacgagtc cggccgctgg agcggcgagc
     2641 ccagggccat cgccaccagg ggcggcgtgg tggaggccgc gatagccgat cccggcgccg
     2701 gcctcgtcag
//
LOCUS       DASSQV010000477         5246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_857624, whole genome shotgun sequence.
ACCESSION   DASSQV010000477 DASSQV010000000
VERSION     DASSQV010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857624"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFL04_08385"
     CDS             <1..834
                     /locus_tag="VFL04_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839764.1"
                     /translation="LLKAVDKSAGMVIGEAQRSGKRPGPAQDRGQRRPPQEGGRGEGR
                     RRQDRRGAAAGAREGGPRDPRRPSRDQATRDDRSPDIRRSIAEATGGSLDFNASSRIE
                     APKPQDRRRSERRQAQPSAKRDGGRRDGRRSGGKPDARPQAHRGEGRRDGQVRQPEAG
                     NPYDMSMEERMRRYREKYGHRLGDEASDQKAAGGKRQGQRPSGKGSGARQDGRRPGQQ
                     RNPPPERGRRDGSGRGRKDASQRSEQGAAPSPGKPGPEQAPEQQKGIFDRFLGAFRKK
                     D"
     gene            985..3522
                     /gene="glgP"
                     /locus_tag="VFL04_08390"
     CDS             985..3522
                     /gene="glgP"
                     /locus_tag="VFL04_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET7839765.1"
                     /translation="MKIVSHTVKPKIPPALASLEEMAHNLWLSWNFQAIMLFIRLDYE
                     VWLKSAQNPAKMLGMVPQERYDELAQDDSYLAALNETAERFERYKQGETWYKGQKDGV
                     VAYFSMEYGLDVSLPTYSGGLGILSGDHMKTASDMGLPLVGVGLLYRQGYFKQYLNPD
                     GFQQELYPENDWYNMPVYRCFDSSGKPILISVEIADRVVAAQIWEVKVGRSSLYLLDT
                     NIEENSQEDRTITAALYGGDKDTRIKQEIVLGIGGIRALNALGIRPAVTHMNEGHSAF
                     LALERIRELMVERKFSFAEAAQVVWPTNIFTTHTPVPAGNERFGIDLMERYFRSWAQH
                     LGLEWKEFLALGRERPDDDGELYCMTVLALKLSAYSNGVSALHGVVSRAMWAGLWPGL
                     PAEEVPISSITNGVHPRTWSSHNMLELLDRYFGPRFYEEPANLDMWDRMDRISDEELW
                     RTHERRRERLVVFARDRLMATMKRSGTHDSALYRAEDALSPSALTIAFARRFATYKRG
                     NLLLRDPERLLRLLSDKQRPVQIIFAGKAHPHDLPGKEIIRELVHFSRKEEVQSRIVF
                     LEDYDMTMSRYLTSGSDVWLNTPRRPLEASGTSGMKAGVNGVLNCSVLDGWWAEGYGP
                     DVGWAIGSGEEYQDEDLQDRIESEALYDLLEREIVPLFYKRGRDGLPRDWIKRMKASI
                     RSTGKEFSTHRMLKEYSERFYFPALDNWRSLAEADFTPAKDVAAYLIRVRKAWPNISI
                     AEIDTDARPIMERGDEVTARALVDLGGLEPQDVAVELYHGTLSNKGEILEPERNQMAA
                     AERKGRLWEYRVTIACGRTGQHGYSARVLPKHPALVSPFVPGLVRWA"
     gene            3665..5038
                     /locus_tag="VFL04_08395"
     CDS             3665..5038
                     /locus_tag="VFL04_08395"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7839766.1"
                     /translation="MASGVLCVLFAMQGQGRASFVCCALFATDAALRTALGGDVPFAR
                     IFPEASLETLARLKAFSAHAAPPLAALFLAFHYPHEGPGILGRQSGRWAVAVAGLAFV
                     VTPVFAPSESLVVGAAYWLVDLCSLGLAGLILGKALERRRLGGLAILIGGLAAAAAAI
                     VDLIPARFEGGRVDYLPYGLALLFGTQVWALVRRLCGILDDAKAGSAELAEVNRRLEE
                     RLQSEAEIRSELEALVAEKELLLREVHHRVKNSLQIVSSIISLQSHRPGEEGMAPLYI
                     SIRNRLRAISLVHEKLFGLAADESVDLAAYARELLGQLAQSYEPVAGEGMLSLSLEHV
                     QVPTDLCVDLGLILTELVANSYLHAVIPQGGGRVRVGLGYADGMISAEVSDDGPGFAS
                     GLPTEGGEGRGFQTALKLARRRGGDIELLPSRGARLMLRVPFEPCSTGWAKAGRAFKK
                     EKGRTHE"
     gene            5031..>5246
                     /locus_tag="VFL04_08400"
     CDS             5031..>5246
                     /locus_tag="VFL04_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7839767.1"
                     /translation="MNDRRDGSEGGYDVAVIGAGVSGANIARRLSAYELRVALIEKEE
                     DVSFGVSKANSGIVHGGFHHDGKYLKAR"
BASE COUNT          922 a         1761 c         1776 g          787 t
ORIGIN      
        1 ctgctcaagg ccgtggacaa gagcgccggc atggtcatcg gggaagcgca gcgctccggg
       61 aagaggccgg gtcccgcgca ggacaggggg cagcgaaggc cgccgcagga ggggggccga
      121 ggcgagggcc gtcgcagaca ggaccgccgc ggagccgccg ccggggcgag ggaaggcggt
      181 ccccgcgacc ctcggagacc atcgagggac caggccacgc gcgatgaccg gagcccagac
      241 atcaggcgca gcatcgcaga ggccacgggc ggcagcctgg atttcaacgc ctcttccagg
      301 atcgaggccc cgaagccgca ggaccgtcgg cgctccgagc gcagacaggc tcagccatcg
      361 gcgaagcggg atggggggcg cagggacggc aggcgctctg gggggaagcc cgatgcccgg
      421 ccccaggccc acaggggcga aggcaggcgt gatggccagg tccggcagcc cgaggccggc
      481 aatccctatg acatgtcgat ggaagagcgg atgcggcgct atcgcgagaa atacggccac
      541 agactcggcg atgaggcgtc ggaccagaag gccgcgggag gcaagcggca gggccaaagg
      601 ccttccggca agggcagcgg agccagacag gatggccgca ggccgggaca gcagcggaat
      661 cctccgcccg agcggggccg ccgcgacggc tctggccgcg gccggaagga cgcgagccag
      721 agatccgagc agggtgcggc cccgagcccc ggcaagcccg ggccggaaca ggcgccggag
      781 cagcagaagg gcatcttcga ccgattcctc ggggccttcc gcaagaagga ctaggagccg
      841 ggccgggccg cgtctgcatg cggcccggcg gtccccatcg caaccccctc aaacttgacg
      901 ctagcccctc cctggactag gctttccgtt gtgtcgcgcg ctcccgagaa gcgcgcggcg
      961 caggaaccac cggaggcggc ggacatgaag atcgtatccc acaccgtaaa accgaagatc
     1021 cccccggcgc tcgcctccct cgaggagatg gcgcacaacc tctggctgtc ctggaacttc
     1081 caggcgatca tgctcttcat ccgcctcgac tacgaggtgt ggctcaagtc ggcccagaac
     1141 ccggcgaaga tgctcgggat ggtcccgcag gagcgctacg acgagctcgc ccaggacgac
     1201 tcctacctcg ctgccctcaa cgagaccgcc gagaggttcg agcgctacaa gcagggcgag
     1261 acctggtaca agggccagaa ggacggtgtg gtcgcctact tttccatgga gtacgggctc
     1321 gacgtctcgc tgcccaccta ctccggcggc ctgggcatcc tctcgggcga ccacatgaag
     1381 accgcgagcg acatgggctt gcccctcgtg ggtgtgggcc tcctctaccg ccagggatac
     1441 ttcaagcagt acctcaaccc ggacggcttc cagcaggagc tctaccccga gaacgactgg
     1501 tacaacatgc cggtctatcg ctgcttcgat tcctcgggca agcccatcct gatctccgtg
     1561 gagatcgccg acagggtcgt cgcggcccag atctgggaag tgaaggtcgg ccgctcatcg
     1621 ctctacctcc tggacaccaa catcgaggag aactcccagg aagacaggac gatcaccgct
     1681 gccctctacg gtggcgacaa ggatacgcgg ataaagcagg agatcgtgct ggggatcgga
     1741 ggcatccgcg ccctgaacgc cctgggcatc aggccggcgg tgacccacat gaacgagggg
     1801 cattcggcct tcctggccct cgagcgcatc agggagctga tggtcgagcg gaagttcagc
     1861 ttcgccgagg cggcccaggt cgtctggccc acgaacatct tcacgaccca cacacccgtg
     1921 cccgcgggga acgagcgttt cggcatcgac ctcatggagc gctacttccg cagctgggcc
     1981 cagcacctgg ggctcgagtg gaaggagttc ctcgccttgg gacgcgagag gccggacgac
     2041 gacggcgagc tctactgcat gacggtcctg gccctcaagc tctcggccta cagcaacggc
     2101 gtgtcggccc tccacggcgt ggtctcgcgc gccatgtggg caggactctg gcccggcctc
     2161 ccggcagaag aggtcccgat atcctcgatc accaacggcg tgcatccgcg cacctggtcc
     2221 tcgcacaaca tgctcgagct gcttgaccgc tacttcgggc cgcgcttcta cgaggagccg
     2281 gcgaacctcg acatgtggga ccgcatggac aggatctccg acgaggagct ctggcgcacc
     2341 catgagcgca ggcgcgagcg tctggtcgtc ttcgcccgcg accggctcat ggccacgatg
     2401 aagcgttcgg gcacccacga ttcggccctc taccgcgccg aggatgccct ctcgccctcg
     2461 gccctcacca tcgcgttcgc acggcgcttc gcgacctata agcgagggaa cctcctcctc
     2521 cgcgatcccg aacgcctgct ccgcctcctg tcggacaagc agcggcccgt gcagatcatc
     2581 ttcgccggca aggcccatcc ccacgacctc cccgggaagg agatcatcag ggagctcgtc
     2641 cacttctccc ggaaggagga ggtccagagc cgcatcgtgt tcctggaaga ctacgacatg
     2701 acgatgtcgc gctatctgac ctcgggttcc gacgtctggc tcaacacccc gaggcgcccc
     2761 ctagaggcat cgggcaccag cgggatgaag gcgggggtca acggcgtcct caactgctcg
     2821 gtgctcgacg gatggtgggc cgagggctat ggacccgatg tcggatgggc gatcggctcg
     2881 ggcgaggaat accaggacga ggacctccag gacaggatcg agagcgaggc cctctacgac
     2941 cttctcgagc gggagatcgt gccccttttc tacaagcgcg ggcgcgatgg cctgcccagg
     3001 gactggatca aaaggatgaa ggcctcgatc cggagcacgg gtaaggaatt ctcgacccac
     3061 aggatgctca aggagtattc cgagcggttc tacttccccg ccttggacaa ttggcgcagc
     3121 ctcgccgagg ccgatttcac gccagcgaag gatgtcgccg cctacctcat ccgggtgcgc
     3181 aaggcctggc caaacatctc catcgcagag atcgacactg acgcgagacc catcatggag
     3241 cggggcgatg aggtgaccgc gagggcgctc gtggatctgg gcggccttga gccccaggac
     3301 gtcgccgtcg agctctacca tggcaccctc tcgaacaagg gcgagatcct ggagccagag
     3361 cgcaaccaga tggcggccgc cgagaggaag ggcaggctct gggagtaccg ggtgacaatc
     3421 gcctgcggca ggaccggcca gcacggctat tcggcccggg tcctgccgaa gcacccggcc
     3481 ctcgtgagcc ccttcgtgcc cgggttggtg cgctgggctt aagcccatgt ggcgcagcag
     3541 ccgcttggca ggctccgccg cggcgttcat ggcgggcctt tcccttcccg tggcccatgc
     3601 ggcccaagtg gcggcgcccg gaccaggaag cgagccctgg acggaattgc tggcaggggc
     3661 cctcgtggca tcaggggtcc tctgcgtcct tttcgccatg cagggccagg ggcgggcgag
     3721 ctttgtgtgc tgcgccctct tcgcgacgga cgcggcgctc aggactgccc tcggcggtga
     3781 tgtccccttc gcgcggattt tccctgaggc cagcctcgag acccttgccc gtctcaaggc
     3841 cttcagcgcc cacgccgccc cgcccctagc cgccctcttc ctcgcattcc actatcccca
     3901 cgaaggaccc ggcatcctcg ggcgccagag cggccgatgg gcggttgcgg tcgccggcct
     3961 ggccttcgtc gtcactccgg tcttcgcccc ctctgagagc ctggtcgttg gcgcagccta
     4021 ctggctggtc gatctctgct ccctcggtct tgctggcctc atcctcggaa aggccctgga
     4081 gcgcagaagg ctcggaggcc ttgccattct gatcggcggt cttgcagcgg cggccgcggc
     4141 catcgtcgac ctgatcccgg cccgcttcga gggtggcagg gtggactacc ttccctatgg
     4201 gctcgccctc cttttcggca cccaggtctg ggccctcgtg aggcggctct gcggcatcct
     4261 cgacgatgcg aaggccggat ccgcggagct ggccgaggtg aaccgtcgcc tcgaggagag
     4321 gctccagagc gaggccgaga tcaggtccga gctcgaggcc ctcgtcgcgg agaaggagct
     4381 cctcctcagg gaagtccatc atcgggtcaa gaacagcctg cagatagtct cgagcatcat
     4441 cagcctccag tcgcatcggc cgggggaaga gggcatggcc ccgctgtaca tctcgatacg
     4501 gaaccgcctg cgcgcgatca gcctggtcca cgagaagctc ttcggactcg ccgcggacga
     4561 gtcggtcgac ctcgctgcct acgcccgcga actcctgggc cagctcgcgc agagctatga
     4621 gcccgtggcc ggggagggca tgctgtccct gtccctcgag cacgtccagg tcccgaccga
     4681 cctctgcgtg gacttgggcc tcatcctgac cgagctcgtc gctaactcct acctgcacgc
     4741 cgtgataccc cagggcgggg gcagggtgag ggttggcctg ggctacgccg atgggatgat
     4801 ctcagccgag gtgagcgacg acggcccggg tttcgccagc ggcctcccaa ccgaaggcgg
     4861 ggaaggcagg ggcttccaga ccgcgctgaa gctggcgagg aggcgggggg gcgacatcga
     4921 gctcctccct tcgcgagggg cccggctcat gctgagggtt cccttcgaac cctgcagtac
     4981 cggctgggcg aaggcaggtc gggcgttcaa gaaggagaag gggcggacac atgaatgatc
     5041 ggcgggatgg ctccgaaggc ggctacgatg tggcggtcat aggcgccggg gtgagcgggg
     5101 cgaacatagc ccggaggctc tcggcatacg agctgcgggt ggccctcatc gagaaggaag
     5161 aggacgtgtc cttcggggtc tccaaggcca actccgggat cgtgcacggc ggcttccacc
     5221 atgacgggaa gtatctcaag gccagg
//
LOCUS       DASSQV010000478         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2350852, whole genome shotgun sequence.
ACCESSION   DASSQV010000478 DASSQV010000000
VERSION     DASSQV010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2350852"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFL04_08405"
     CDS             complement(<1..552)
                     /locus_tag="VFL04_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET7839768.1"
                     /translation="MKENDSATDAPVAEGPVGEGRLFRLKDFEGPLDLLLFLIKKNEV
                     SVYDIPIAAITEQYLGFLSNGEDVDLEDLTEFYSMAATLLYIKSRMLLPVQIDLDDEL
                     EDPRRELIEKLIEYQKFKRLAELMERKEMEVEWSVERKRIQRPLPFAEDQTLWEEIDV
                     WDLLRSFSSLVTNLSSERIIDLYE"
     gene            complement(558..1067)
                     /gene="folK"
                     /locus_tag="VFL04_08410"
     CDS             complement(558..1067)
                     /gene="folK"
                     /locus_tag="VFL04_08410"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7839769.1"
                     /translation="MALVFLGLGSNVGDSVATIKAAFAELAGELRNPKFSRLWRSRPR
                     YMEDQPDFVNAAIAGETDLEPRALLGMIQGIEAAHGRDRSREQFKGPRPLDVDILLYG
                     DRLIAEPDLLIPHPGLRERKFALLPLLDIAPRLVDPATGVRFSAIAASLPAQGIYLLG
                     GDGYDYALA"
     gene            complement(1071..2297)
                     /gene="recA"
                     /locus_tag="VFL04_08415"
     CDS             complement(1071..2297)
                     /gene="recA"
                     /locus_tag="VFL04_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET7839770.1"
                     /translation="MGEKIKALEAARLQIEKQFGMGSLMKLGANEHAPGIDSVPTGSI
                     LLDEALGVGGYPRGRVIEIYGPESSGKTTLALHAIAEAQKKGGIAAFIDAEHALDPVY
                     ARNLGVNTDELWVSQPDNGEQALDITESLIRSGAVDIIVIDSVAALTPQAEIEGDMGD
                     AHVGLQARLMSQALRKLTGTLSKSKTILVFINQIRMKIGVMFGNPETTTGGNALKFYS
                     SVRLEVRKVETIEKGEEEAVGNKVRVKVVKNKVAPPFRKVELEIMFGKGISWAGSLID
                     AAVKYNIVDKKGAWYTWGEEKVGQGRDNAKLYLETNAQAAAEIERQVRSLIFSAQHPE
                     SAQAPDGGGRGSPATSQSEQPSPKPATAVVAEKPAGRGPGRPPKQPSAPEPAPVQARP
                     AAAGAKSPPADDDGLF"
     gene            complement(2449..>2681)
                     /locus_tag="VFL04_08420"
     CDS             complement(2449..>2681)
                     /locus_tag="VFL04_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839771.1"
                     /translation="LNGAACLLDLLSGLAPTEPPFRLLDPSYYLERIPVRISPMEIGL
                     VAAACLLLCLLASLLPARRAARLPPMEIMRRT"
BASE COUNT          435 a          809 c          889 g          548 t
ORIGIN      
        1 ctcatacagg tcaattatcc gctccgagga caggttggtg accagcgagg agaaggagcg
       61 gaggaggtcc cagacatcga tctcctccca tagggtctgg tcctcggcga agggcagggg
      121 gcgctggatg cgtttgcgct cgacgctcca ctcgacctcc atctccttgc gctccatgag
      181 ctcggcgagg cgcttgaact tctggtactc gatcagcttt tcgatgagct ccctgcgggg
      241 gtcttccagc tcatcgtcga ggtcgatctg gaccgggagc agcatcctcg acttgatgta
      301 gagaagggtg gcggccatgg agtagaactc ggtgagatcc tcgagatcga catcctcgcc
      361 gttgctcagg aagccaaggt actgctccgt gatcgccgcg atggggatgt cgtagacgct
      421 cacctcgttc ttctttatga ggaatagcag gagatcgagc ggtccctcga agtccttcag
      481 gcggaagagc ctgccctccc ctaccgggcc ctcggcgacc ggggcgtccg tggcactatc
      541 attctctttc attattgcta cgcaagggca taatcatagc cgtcgccacc cagaaggtaa
      601 ataccctggg cggggagaga ggccgcaatg gctgagaagc ggaccccggt cgccgggtcg
      661 acgagcctgg gggcgatgtc cagcaggggc agcaaggcga acttcctctc tctgagtcct
      721 ggatggggga tgaggaggtc tggctcggcg attagcctgt ccccgtacag gaggatatct
      781 acgtcgaggg gacgggggcc cttgaactgt tcccgggacc ggtctcggcc atgggcagcc
      841 tcgatgccct gaatcatgcc taggagggct cttggttcca gatcggtctc gccggcgatg
      901 gcggcattca cgaaatccgg ctggtcctcc atgtaacggg gccgggagcg ccagagtctc
      961 gagaatttcg ggtttcgcag ctcgccggcg agctcggcga aggcggcctt gatagtcgcc
     1021 acgctatcgc cgacattcga tccgaggcca agaaagacca gggccatgct ctagaacagg
     1081 ccgtcgtcgt ctgccggagg cgatttcgcc ccggctgcag ccggtcgggc ctggactggg
     1141 gccggctcgg gagcgctggg ctgtttgggg ggccgccccg gtccgcggcc agccggtttc
     1201 tcggccacga cagcggtggc gggtttcggg ctgggctgct cgctctggct ggtcgcgggc
     1261 gagcctcggc cccctccatc gggggcctgg gcactctcgg gatgctgggc gctgaatatc
     1321 aggctcctga cctgccgctc gatctcggcg gcggcctggg cgttcgtctc cagatagagc
     1381 ttcgcgttgt cgcgtccctg gcccaccttt tcctcgcccc aggtgtacca ggcccctttc
     1441 ttgtcgacaa tgttgtattt gactgcagcg tcgatgaggg atcccgccca ggagatgcct
     1501 tttccgaaca tgatctccag ctctaccttc cggaagggcg gggctacctt gttcttgacc
     1561 accttgacgc ggaccttgtt gcccaccgcc tcttcctcgc ccttctctat cgtctcgacc
     1621 ttgcggacct cgagccggac cgaggagtag aacttgagcg cgttgccccc cgttgtggtc
     1681 tcggggttac cgaacatcac cccgatcttc attcggatct ggttgatgaa gacgaggatc
     1741 gtcttgctct tggacagcgt gcccgtgagc ttgcgcaggg cctggctcat gagtctggcc
     1801 tgaagtccga cgtgggcatc gcccatgtcg ccctcgatct cggcctgggg tgtgagggct
     1861 gcgacggaat cgatgacgat gatgtccact gcccctgacc tgatcaggga ttcggtgatg
     1921 tcgagggcct gctccccgtt gtccggctgg gacacccaga gctcgtcggt gtttacgccg
     1981 aggttcctgg catagacggg atcgagggcg tgttcggcgt ctatgaaggc ggcaattccg
     2041 cccttcttct gggcctcggc gatggcatgg agggcgagag tcgtcttgcc cgaggattcg
     2101 gggccataga tctcgatgac acgaccgcgc ggatagcctc cgacccctag ggcctcgtcc
     2161 aggaggatgg agcccgtggg gacggagtcg atccccggcg cgtgctcgtt ggcgccgagc
     2221 ttcatgaggg atcccatgcc gaactgcttc tctatctgga gtcgagcggc ttctagcgcc
     2281 ttgatcttct cgcccatctc agtgatcttc tcgctggatt gggccgcttc ctttttcacg
     2341 gcataccctc cgacctcacg acgcacgccc gtctcccgga cgattgaagt ccggcgacac
     2401 tatacatgct tttggcggga atttgaaggg cgcaggggtc cctggccctc aggtcctcct
     2461 catgatctcc atggggggga ggcgggcggc gcgccgcgcg gggagaagcg aggcgagcag
     2521 gcagagaagg agacaggcgg cagccacaag gccgatttcc atggggctga tccttaccgg
     2581 aatcctctcg agatagtagg agggatcgag gagcctgaaa ggaggctcgg tgggcgcgag
     2641 cccggacagg aggtcgagga ggcaggccgc cccgttcagg a
//
LOCUS       DASSQV010000479         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2350920, whole genome shotgun sequence.
ACCESSION   DASSQV010000479 DASSQV010000000
VERSION     DASSQV010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2350920"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFL04_08425"
     CDS             complement(<1..414)
                     /locus_tag="VFL04_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7839772.1"
                     /translation="MSDSFMDTYKRTGLVLAKGSGSRVTDTGGREYIDFAAGIGVNSL
                     GHAHPALVAAIAAQASRLIHVSNYYQTAEALAMAAELCAATGYERVFLCNSGAEANDG
                     AMKVARKYGSLKSPARTTIVALWGSFHGRTIAGLAA"
     gene            complement(411..1274)
                     /gene="argB"
                     /locus_tag="VFL04_08430"
     CDS             complement(411..1274)
                     /gene="argB"
                     /locus_tag="VFL04_08430"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET7839773.1"
                     /translation="MSFSDSARAEALASALPFMRSLAGKTVVVKYGGAAMTEEAARLA
                     LAEDLVLMASVGIRTVLVHGGGPEIDAMLRRVGKEPVFIGGLRRTDEETMELVQMVLA
                     GKVNKGLVALIQRKGGRAVGLCGLDGGLLQARKLREGPDLGLVGEIVAVDTGLITAAL
                     AAACIAVVATVAAGLPGEAEVYNVNADTAAAELALALGAERLLLLTDVPGILLDIKDE
                     GSLVREIGRGELDGYIARGIVSKGMIPKLRACVRALEGGVKSAHIIDGRAPHGLLLEL
                     FSEGGKGTMIS"
     gene            complement(1288..2328)
                     /gene="argC"
                     /locus_tag="VFL04_08435"
     CDS             complement(1288..2328)
                     /gene="argC"
                     /locus_tag="VFL04_08435"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET7839774.1"
                     /translation="MVRCGVVGATGYAGAELARLLVSHPEVAGLSLGSSSSVGEEMER
                     IYPNLGGAFPQTRLVDRASVVEESDVVFSSLPHGHAEEIAALCAAAGKLFIDLSADFR
                     FGEDETAYSAWYGRNYSHPSLHAASVYGLPELNRERIAKARIIGNPGCYPTASTLGLF
                     PALRLGFAAHDGIVIDAKSGVTGAGREPTRSTHYAEVADSLVPYKIGSHRHTPEIAAV
                     LSAMASSPVSAIFTPHLAPMGRGIVATIYFRLAQQVEVERLRQAYADFYAREPFVRVL
                     PEGTVATNRNVRLSNYCDISVHLGSDGRTAIVVSAIDNMVKGAAGQAIQNMNIAMGFD
                     EKAGIAMMPPAF"
     gene            complement(2282..>2497)
                     /locus_tag="VFL04_08440"
     CDS             complement(2282..>2497)
                     /locus_tag="VFL04_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839775.1"
                     /translation="EADYAFGGNGVKTSSRPGKRINMGPNCMNMHEALTSDAFSDIFR
                     IKMNIAQAKEDADGTLRRRWRDRLCRR"
BASE COUNT          376 a          833 c          886 g          402 t
ORIGIN      
        1 ggcggccaag cccgcgatgg tcctgccgtg gaaggagccc cagagggcca cgatcgtggt
       61 ccgcgcgggg ctcttcagcg agccgtactt gcgcgccacc ttcatggcgc cgtcgttggc
      121 ctcggccccg gagttgcaca gaaagacgcg ctcgtagccc gtcgccgcgc agagctccgc
      181 ggccatggcg agggcctcgg ccgtctggta gtagttcgag acgtggatga ggcgggaggc
      241 ctgggcggcg atcgcggcca ccagggccgg gtgggcgtgg cccagggagt tgaccccgat
      301 gcccgccgcg aagtcgatgt actccctgcc cccggtgtcg gttaccctac tgcccgagcc
      361 cttcgcgagg acgaggccgg tgcgcttgta ggtgtccatg aaggaatcgc tcatgagatc
      421 atcgtcccct ttccgccctc ggagaacagc tcgaggagga gtccgtgcgg cgccctgccg
      481 tcgatgatgt gcgcgctctt gaccccgccc tcgagggcgc ggacgcaggc gcgcagcttg
      541 ggtatcatgc ccttggagac tatgccccgg gcgatgtagc cgtccagctc tcctcggcct
      601 atctcgcgga ccaggctccc ctcgtccttg atgtccagca gtatcccggg cacgtcggtg
      661 aggaggagga ggcgctcggc cccaagggcc agggcgagct cggccgcggc cgtgtcggcg
      721 ttcacgttgt agacctcggc ctcgccgggc aggccggcgg ccacggtcgc gacgacggcg
      781 atgcaggccg ccgccagggc cgcggtgatc aggcccgtgt ccacggcgac gatctcgccc
      841 acgaggccaa ggtcgggccc ctccctcagc ttcctcgcct ggaggaggcc gccgtccagg
      901 ccgcagagtc cgacagccct gccccccttc ctctggatga gggcgacgag gcccttgttc
      961 accttgcccg cgaggaccat ctggacaagc tccatggtct cctcgtcggt gcgtctgagc
     1021 cccccgatga agacgggctc cttgcccacc cgcctcagca tcgcgtcgat ctcgggcccg
     1081 ccgccgtgga cgaggaccgt cctgatcccg accgaggcca tgaggacgag atcctcggcc
     1141 agggccagcc tcgccgcctc ctcggtcatg gcggcgccgc cgtacttcac gaccaccgtc
     1201 ttccccgcca ggctccgcat gaagggcagg gccgaggcga gggcctcggc cctcgcgctg
     1261 tcggaaaagc tcacagccac ccccttccta aaaggccggc ggcatcatgg cgatgcccgc
     1321 cttctcgtcg aagcccatgg cgatgttcat gttctggatc gcctggcccg ccgcgccctt
     1381 gaccatgttg tcgatggccg agacgacgat cgcagtccgc ccgtccgagc cgaggtggac
     1441 cgagatgtcg cagtagttcg agagcctcac gttcctgttg gtggcgaccg tgccctcggg
     1501 caggacccgc acgaagggct ccctggcgta gaagtcggcg taggcctggc ggagccgctc
     1561 cacctcgacc tgctgggcaa ggcggaaata gatcgtggcc acgatgccgc ggcccatggg
     1621 cgcgaggtgg ggcgtgaaga tggccgagac gggggagctc gccatggccg acaggacggc
     1681 ggcgatctcg ggggtatggc ggtgggagcc gatcttgtag ggcacaaggg agtcggccac
     1741 ctcggcgtag tgggtggagc gcgtcggctc gcggccagcc ccggtgacgc ccgacttcgc
     1801 gtcgatgacg atgccgtcgt gggccgcgaa gccgaggcgg agggcgggga agaggccgag
     1861 ggtcgaggct gtggggtagc agccggggtt accgatgatg cgggccttag cgatgcgctc
     1921 gcggttgagc tcggggaggc cgtagaccga ggccgcgtgg aggctcgggt gcgagtagtt
     1981 cctgccgtac caggccgagt acgcggtctc gtcctcgccg aagcggaagt cggccgagag
     2041 gtcgatgaag agcttcccgg cggcggcgca gagggcggcg atctcctcgg cgtggccgtg
     2101 ggggagggag gagaagacca cgtcggactc ctcgactacg ctggcgcggt cgacgaggcg
     2161 ggtctggggg aaggccccgc cgaggttggg gtagatcctc tccatctcct cgcccaccga
     2221 gctcgaggag cccaggctca gcccggcgac ctcggggtgg gagacgagaa gcctcgcgag
     2281 ctcagcgccg gcatagccgg tcgcgccaac gacgccgcag cgtaccatca gcatcctcct
     2341 tggcctgcgc gatattcatc tttatgcgga atatgtcaga aaacgcatcc gatgtcaacg
     2401 cttcatgcat attcatgcag tttggcccca tattgatccg tttgccgggc cgggagcttg
     2461 tcttgacgcc atttccgccg aaagcgtagt ctgcctc
//
LOCUS       DASSQV010000480         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_858156, whole genome shotgun sequence.
ACCESSION   DASSQV010000480 DASSQV010000000
VERSION     DASSQV010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858156"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFL04_08445"
     CDS             complement(<1..385)
                     /locus_tag="VFL04_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET7839776.1"
                     /translation="MSPELSVSIGGALLPNPVGVASGTFGYGSEYEDLVELGALGALY
                     TKAVTLEPRQGNDVPRIVETPAGMLNSIGLANPGVEAFLATKLPALLACPCPVIVNVA
                     GATEEEYFEVLQRIEAALGAPSGDSP"
     gene            complement(382..1164)
                     /locus_tag="VFL04_08450"
     CDS             complement(382..1164)
                     /locus_tag="VFL04_08450"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET7839777.1"
                     /translation="MKQFEAEIISNRSVAPGWKELVLSWEPGAGRPEPGQFLSLRVSP
                     RSDPLLRRPFAFSDLSEDGSRASAIYQVRGPATSILAELAPGSAIDAIGPLGRGFPRP
                     MPGELPILAAGGIGLGPVLFLARSLAAPTLVLGFRDASALPDLELPEGRVTCTDDGSA
                     GFHGNPAEWIARNVSADLVRLYGCGPAPMLAALAGLAAERSWATSLSAEQWMACGVGA
                     CMGCALRRSDGKGYLRACSDGPVFSARSGEASPVDWNPRRPA"
     gene            1241..2227
                     /locus_tag="VFL04_08455"
     CDS             1241..2227
                     /locus_tag="VFL04_08455"
                     /inference="COORDINATES: protein motif:HMM:NF015889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HET7839778.1"
                     /translation="MDGLYDILILSTLLLLGAAAPRLRVGGRRILALPPKAVDLVLRL
                     IIWALLLAMGFRLGNDRELAARIGEIGLLAFSSAFLALAGTVAAIVLSSLAMQRLSGA
                     PGSGAPVPEAPPAAGSPGERLRLILSNLKAPATLLGFVAGGFLLGIALPRLASLDLAS
                     ISAWILKALLFFIGMQFSQSGFSLKGAFFKAENLIVPAATMVGSFAGGLLLVPIFGLA
                     GGKALALVGGFGWYSLSGVLISDLGDPVLGSASFLSNMVRESVALLCIPFLARTRFPT
                     TAVGLGGATSMDVTLPIIYASAGPAMVPVSFVSGAILSASVPLLVPLCFLIK"
     gene            2348..2647
                     /locus_tag="VFL04_08460"
     CDS             2348..2647
                     /locus_tag="VFL04_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839779.1"
                     /translation="MVAISKDQLFGLFPVFVLDLSAEGLGREGAFPYSRKSNNNSVEF
                     MVWRNKSELELFVKRNGSFKNNFEIREVDRDNYSQVIESRLPKDRLPQFLLIDGR"
     gene            complement(2655..>3347)
                     /locus_tag="VFL04_08465"
     CDS             complement(2655..>3347)
                     /locus_tag="VFL04_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET7839780.1"
                     /translation="CAGDSITRGAVSADYVQLLASRLPGLDFVNAGVNSELAWNLASR
                     LDEIVALQPEAVTVLIGTNDVNATFGLRSVLGYYAIHRLPERPNPLFFRENLTLIVRR
                     LKRDTGARIGLFSIPPIGEESGHYAYLRTEEYSRIIREIAKEEGTGYLPLRERMCAYL
                     EALPKGNPLPFRRIGAAQRRAVRARLMLGRSLDEISASNGFHLLVDGIHLNTHAASMA
                     ADLAESFLLAAP"
BASE COUNT          472 a         1150 c         1179 g          546 t
ORIGIN      
        1 ccggggagtc gcccgagggg gcgcccaggg cggcctctat cctctggagg acctcgaagt
       61 actcctcctc ggtggcgccg gcgacgttga cgatgacggg gcaggggcag gccaggaggg
      121 ctggcagctt cgtggcgagg aaggcctcga cccccgggtt cgcgaggccg atggagttga
      181 gcatgcccgc cggggtctcc acgatgcgcg gcacatcgtt gccctgcctg ggctcgaggg
      241 tgaccgcctt ggtgtacagg gcgccgaggg cgccgagctc gacgagatcc tcgtactcgg
      301 agccgtagcc gaaggtgccc gaggccacgc ccacggggtt gggcaggagg gcgccgccga
      361 tgctgaccga gagctcgggg ctcacgccgg cctccttggg ttccagtcca cgggcgaggc
      421 ctcgccggag cgggcgctga acaccgggcc gtcggagcag gcgcggaggt agcccttccc
      481 gtcggagcgc ctgagggcgc agcccatgca ggccccgacc ccgcaggcca tccactgctc
      541 ggccgagagg ctcgtggccc aggagcgctc ggccgcgagc ccggccaggg cggccagcat
      601 gggagccggg ccgcagccgt agaggcggac caggtcagcc gacacgttgc gcgctatcca
      661 ctcggccggg ttgccgtgga agccggccga gccgtcgtcg gtgcaggtca ccctgccctc
      721 gggaagctcg agatcgggca gggccgaggc atcgcggaag ccgaggacca gggtgggggc
      781 ggcgaggctc cgcgcgagga agaggactgg cccgaggccg atgcccccgg ccgccagaat
      841 gggcagttcc ccgggcatgg gcctggggaa gccgcggcca agcggtccga tcgcgtcgat
      901 ggccgagccc ggggcgagct cggcgaggat cgaggtggcc ggccccctga cttggtagat
      961 cgccgaggcc cgcgacccgt cctccgacag gtccgagaag gcgaagggcc gtctgaggag
     1021 gggatcgctg cggggcgaga ccctgaggct gaggaactgc cccggctcgg gcctgccggc
     1081 cccgggctcc caggagagga ccagctcctt ccatccgggc gccacgctcc ggttggagat
     1141 gatctcggct tcgaactgct tcacgcgcgc aacagtagac gcgggacggg gtcgtgtcaa
     1201 ccttgcgcag gagcgcgctt cgcctgtatg ctccgtcccg atggacgggc tctacgacat
     1261 cctcatcctc tcgaccctcc ttctgctcgg ggcggccgcg ccccggctca gggtcggggg
     1321 gcgccggatc ctcgccctgc ctccgaaggc tgtcgacctc gtcctcaggc tcatcatctg
     1381 ggccctcctc ctcgcgatgg gcttccggct cgggaacgac agggagctcg ccgcccggat
     1441 cggggagata ggcctcctag ccttctcgag cgccttcctc gccctcgccg ggacggtggc
     1501 ggccatcgtc ctctcgagcc tcgccatgca aaggctctcc ggggccccgg ggtcgggggc
     1561 gccggtcccc gaggccccgc ccgccgcagg gagtcctggc gagcgcttga ggctcatcct
     1621 ctcgaacctc aaggcccccg ccaccctcct cggctttgtg gccggaggct tcctcctcgg
     1681 catcgccctg cccaggctag ccagcctcga cctggcctcg atctcggcct ggatcctcaa
     1741 ggccctcctc ttcttcattg gcatgcagtt ctcccagtcg ggcttctcgc tcaagggcgc
     1801 cttcttcaag gccgagaacc tcatcgtgcc cgcggcgacc atggtcggca gcttcgccgg
     1861 cggcctcctc ctcgtgccga ttttcggcct ggcgggcggc aaggccctcg ccctcgtggg
     1921 gggcttcggc tggtacagcc tctcgggggt cctcatctcc gacctcggcg accccgtcct
     1981 cggctcggcc tccttcctct ccaacatggt cagggagtcg gtggccctgc tctgcatccc
     2041 cttcctcgcg aggacccgct tccccacgac ggccgtgggt ctgggcgggg cgacctcgat
     2101 ggacgtcacc ctgccgatca tctacgcgag cgcgggcccc gccatggtgc cggtgagctt
     2161 cgtgagcggg gccatcctga gcgcctccgt gcccctcctc gtccccctct gcttcctcat
     2221 aaagtgaggc tgtcccggcc cgcccgggac caacaggctc cgaggcagcg cttaaaactt
     2281 gcttttgcgg aattccttgc attatccttg agtctggaat ttttgggacc acggagggct
     2341 tttatcgatg gtggctatct cgaaggacca gctcttcggt ctcttccccg tcttcgtcct
     2401 cgacctctcg gccgagggcc tcgggaggga gggcgccttt ccctactcgc gcaagagcaa
     2461 caacaactcg gtcgagttca tggtgtggcg caacaagtcc gagctcgagc tcttcgtgaa
     2521 gcgcaacggc tccttcaaga acaacttcga gatccgcgag gtcgaccgcg acaactactc
     2581 ccaggtcatc gaatcccgcc tccccaagga caggcttccc caattcctcc tgatagacgg
     2641 ccgctagctc ggctctaggg cgcggcgagg aggaaggact cggccaggtc ggcggccatc
     2701 gaggccgcgt gggtgttgag gtgtatcccg tccacgagga ggtggaagcc gttcgaggcc
     2761 gagatctcgt cgaggctcct gccgagcatg aggcgggcgc gcacggcccg cctctgggcg
     2821 gcgccgatcc ggcggaaggg gaggggattg cccttgggca gggcctcgag gtaggcgcac
     2881 atccgctcgc gcaggggcag atagcccgtg ccctcctcct tggcgatctc ccttatgatc
     2941 cgcgaatact cctcggtgcg gaggtaggcg tagtgcccgc tctcctcgcc gatgggcggg
     3001 atcgagaaga gtccgatgcg ggcccccgtg tcgcgcttga gcctgcgcac gatgagggtg
     3061 aggttctcgc ggaagaagag ggggtttggg cgctcgggca gacggtggat ggcgtagtag
     3121 ccgaggacag agcgcaggcc gaaggtggcg ttgacgtcgt tggtcccgat gaggacggtc
     3181 accgcctcgg gctggagggc gacgatctcg tcgagcctcg aggcgaggtt ccaggccagc
     3241 tcggagttga cgccggcgtt cacgaagtcc aggccgggca ggcgggaggc gaggagctgg
     3301 acgtagtcgg cgctgaccgc gcccctcgtg atcgagtccc cggcgca
//
LOCUS       DASSQV010000481         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1478936, whole genome shotgun sequence.
ACCESSION   DASSQV010000481 DASSQV010000000
VERSION     DASSQV010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478936"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFL04_08470"
     CDS             complement(<1..623)
                     /locus_tag="VFL04_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET7839781.1"
                     /translation="MRLLADLHNHSCLSPCASLEMSPRRIARTARERGIGLLGLTDHN
                     AALNCPAFAEACRLEGIVPLFGIEASSIEEVHVLVLFDAVEAALDFGSLLHAHLPRVR
                     LDRRRFGDQVVVNEAEEVLELPDQYLGMALDLGFDELCAEGARRSGLVIPAHIDRPMF
                     SVSSQLGFLPEGPYDALESMREPQKAICRGYPLVSGSDAHYPEHVGR"
     gene            complement(631..972)
                     /locus_tag="VFL04_08475"
     CDS             complement(631..972)
                     /locus_tag="VFL04_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839782.1"
                     /translation="MLISELGTELGLACVQAVFPDRPLAGAYTSDLLSDVMANAGSGA
                     ALVTIQAHRNTIAVASLTGIAAVVVCNGREPPGDMLEAAREEGIAVFSTGQSQFEVSG
                     RLWDALRRDGA"
     gene            1041..2249
                     /locus_tag="VFL04_08480"
     CDS             1041..2249
                     /locus_tag="VFL04_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7839783.1"
                     /translation="MRTKTRKSLGLLTLLLAFLPSLAAERFAFVHVKGDKFRVLSQVS
                     EDVYINRKLSHGAEILNRISFEVTDAAPDGSWGELTGTFETSERRKGESAYLVAESYD
                     SKFRRDRLGRYTIPSEYYMPVVRNVPVFPDRDLAVGDTWTAPGEERHDLRAFFGIPDP
                     YSIPFEARYRYEGPSAKDGKDLRLITVSYTIFTRPGPPAAYDKVYPVQIAGYSDQRIW
                     WDPALGQPVSYEERFDLVFDWSDGTTIEYRGQAGSDLVVAELMDRDRVRADVEKAVAG
                     MPNVSVAKTPEGVTISIQDIQFMADSAVLAPEELDKLGKIAEILKRYPDRDVLVAGHS
                     AAAGYAPGRKKLSEDRAQAVVERLVAAGARSPDRIRAIGYGDERPVADNATAEGRARN
                     RRVEITILEN"
     gene            complement(2263..2715)
                     /locus_tag="VFL04_08485"
     CDS             complement(2263..2715)
                     /locus_tag="VFL04_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HET7839784.1"
                     /translation="MNQIAPASEPLLLLLAILAVALLVALFVGGLVIGLRLGRNSGRL
                     EAERGLPARLEEERGDAVRRSRAVLGGQLAEQIAPYLPGFPFDPGELRFIGKPVDFLA
                     FVGASRGMVEEVAFIEVKTGLAGLSKVERSLRDAVKAGRVRWVEDRAP"
     gene            2805..>3233
                     /locus_tag="VFL04_08490"
     CDS             2805..>3233
                     /locus_tag="VFL04_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839785.1"
                     /translation="MPFLPVNRDELKARGFDELDAVLVTGDAYVDHPSFGAALIGRLL
                     EAEGYRVGIIARPDPDDVEAFRLLGRPRLAFLVTAGAIDSMVSSYTANKKPRTEDDYA
                     PGGKAELCLRGDGTIGIGKLGRPQARPDRAVIAYATKCREA"
BASE COUNT          556 a         1108 c         1100 g          469 t
ORIGIN      
        1 cttctcccta cgtgctcggg atagtgggca tccgagcccg agacgagggg atagccgcgg
       61 cagatcgcct tctgcggctc gcgcatggat tcgagggcgt cgtagggccc ctcggggagg
      121 aagccgagct ggctcgagac cgagaacatc ggcctgtcga tgtgggccgg gatgacaagg
      181 ccggaccgcc tcgccccctc ggcgcagagc tcatcgaagc cgaggtccag ggccatgccg
      241 agatactgat cgggcagctc gagcacctcc tcggcctcat tcaccacgac ctggtccccg
      301 aacctcctcc tgtcgagcct gaccctcggg agatgggcgt gcaggaggga cccgaaatcc
      361 agggcagcct cgacggcgtc gaagaggacg aggacgtgga cttcctcgat gctcgaggcc
      421 tcgatcccga agagggggac gatcccctcg agccggcagg cctcggcgaa ggcggggcag
      481 ttcagggcgg cgttgtggtc ggtcaggccg aggaggccaa tccccctctc cctggccgtc
      541 ctggcaatgc gccttggcga catctcgagg ctcgcgcagg gcgagagaca gctgtggttg
      601 tggaggtcgg caaggagccg caccgggacc tcaggccccg tcccttctca gggcatccca
      661 gagcctgccc gacacctcga actggctctg tccggtcgag aagaccgcga tcccctcctc
      721 gcgggcggcc tcgagcatgt cccccggggg ctcgcggccg ttgcagacca cgaccgcggc
      781 gatgcccgtg aggctcgcca cggcgatcgt gttgcggtgg gcctggatcg tcacgagggc
      841 ggcccctgat cccgcattgg ccatgacgtc cgagagcagg tccgaggtgt aggctccggc
      901 cagggggcgg tcagggaaaa cggcctggac gcaggcgagt ccgagctcgg ttcccagttc
      961 gctgatcagc atgatgtctc tcctgaaggc cttggggatt ccgcatggga ttatgcccgg
     1021 ctcatgtcga tattggaagc atgcgcacga agacacgcaa atccctcggc ctcctgaccc
     1081 tcctcctcgc cttcctaccc tccctcgccg ctgagcgctt cgccttcgtc cacgtcaaag
     1141 gggacaagtt ccgcgtgctt tcgcaggtgt cggaggacgt ctacataaac cgcaagctct
     1201 cccacggggc ggagatattg aacaggatat ccttcgaggt cacggacgcc gccccggacg
     1261 ggagctgggg cgagctcacg gggaccttcg agacctcgga gcggcgcaag ggggagagcg
     1321 cctatctggt cgccgaatcc tacgactcga agttccgccg cgaccgcctc ggccgctaca
     1381 cgatcccgag cgagtactac atgccggtgg tgaggaacgt gccggtgttc cccgaccggg
     1441 acctcgccgt cggcgacacc tggaccgccc ccggagagga acggcacgac ctccgcgcct
     1501 tcttcggcat accggatccc tactcgatcc ccttcgaggc gcgctaccgc tacgagggac
     1561 cgagcgcgaa ggacggcaag gaccttagac tgatcaccgt ctcctacacg atcttcaccc
     1621 gccccggccc gcccgcggcc tatgacaagg tctaccccgt ccagatcgcc ggctattccg
     1681 accaaaggat ctggtgggat cctgccctgg gacagcccgt ctcctacgag gagcgcttcg
     1741 acctggtctt cgactggtcg gacgggacca cgatcgagta ccggggccag gccggctccg
     1801 acctcgtcgt cgccgagctc atggaccgcg acagggtcag ggccgacgtg gagaaggccg
     1861 tcgcgggcat gcccaacgtc tcagtggcga agacccccga gggcgtgacg atcagcatcc
     1921 aggacatcca gttcatggcc gactctgccg tccttgcccc tgaggagctc gacaagctgg
     1981 gcaagatagc cgagatcctc aagcgctacc ccgaccggga cgtcctcgtg gccggccact
     2041 cggctgcggc gggctatgcg cccggccgga agaagctctc cgaggatagg gcccaggcag
     2101 tcgtcgagcg cctcgtggcc gccggagcgc ggagccccga ccggatcagg gccatcggct
     2161 acggagacga aaggccggtg gcggacaacg ccacggccga gggcagggcg aggaaccgcc
     2221 gcgtcgagat caccatcctg gagaactagg gggccggtcg cactaggggg cccggtcctc
     2281 cacccagcgc acccttccgg ccttgacggc atcgcggaga ctgcgctcga ccttgctcag
     2341 accggccagc cccgtcttca cctcgatgaa ggcgacctcc tcgaccatgc cccgggaggc
     2401 gccgacaaag gccaggaaat cgacgggctt gccgatgaac ctgagctccc cggggtcgaa
     2461 ggggaagccg gggaggtagg gagcgatctg ctccgcgagc tggccgccga ggacggcccg
     2521 ggagcggcgc acggcgtcgc cgcgctcctc ctcgagccgg gccggcagac cgcgctcggc
     2581 ctccagcctg cccgagttcc gcccaagcct caggccgatg acgaggccgc ccacgaagag
     2641 ggcgacgaga agggcgacgg cgaggatggc gaggagaagc aggaggggct ccgatgcggg
     2701 tgctatctgg ttcatggcag ggcggagtgt agctcggggc ggggccctcg gctagggaag
     2761 aagcggcaga cgaggaagcg ggcccggcgc tatactcagg gcccatgccc ttccttcccg
     2821 tgaaccgaga cgagcttaag gcccgcggct tcgacgagct cgacgcggtc ctcgtcacgg
     2881 gcgacgccta tgtcgaccac ccctccttcg gtgcggccct cattggccgc ctccttgagg
     2941 ccgagggata cagggtcggc atcatcgcca ggcccgaccc cgacgacgtg gaggccttca
     3001 ggctgctcgg gaggccgagg ctggccttcc tcgtcaccgc gggagcgatc gactcgatgg
     3061 tctcctccta cacggcgaac aagaagccga ggaccgagga cgactatgcc cccggcggca
     3121 aggctgagct ctgtctgcgc ggcgacggta cgatcggaat cggaaagctg gggcggcccc
     3181 aggcccgccc ggaccgcgcg gtcatcgcct acgccaccaa gtgccgcgag gcc
//
LOCUS       DASSQV010000482         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_609475, whole genome shotgun sequence.
ACCESSION   DASSQV010000482 DASSQV010000000
VERSION     DASSQV010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609475"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..924)
                     /locus_tag="VFL04_08495"
     CDS             complement(169..924)
                     /locus_tag="VFL04_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839786.1"
                     /translation="MASPGISLVAALAAGLLLSASLSCSRASPASRAQTSSGALASPA
                     QTPAPQGPSPLLAAPQTPAGASARAPAPGPQEAPKTGSPSLPPQPAKPKPRPVLAASL
                     RAFGLSANAAIMPEDFSIGALQDYRSGSDRLSSALATAQGLMDGLAKGKLDPALLVPE
                     EREALSLALAPLSGEMQGGDFRYGQISLSGDEAWMRVRLAPAGDGPSHEGLLSLRADG
                     EVWRVESLSFAVAAKARGSTFEPGMPPALGPGD"
     gene            1021..2349
                     /locus_tag="VFL04_08500"
     CDS             1021..2349
                     /locus_tag="VFL04_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7839787.1"
                     /translation="MKSGALLEHLASSFTEPVRDPLWGNIFLSPGLEALANSGPFAKL
                     ARIKQLGPAHLVYPGATHTRRAHSLGVFHMARRLCQALVARGGIEFVTREGLDSFLAA
                     ALCHDLGHFPFAHSLKELPLADHEALTARLLVEEPLRSLVGTSGADPEATAAIVDQDL
                     PDRGDREIRFFRGLLSGVLDPDKLDYLTRDAFFCGVPYGIQDSDFILQRVGIAEEDRL
                     GIEERGLMSVESLLFSKYLMYRSVYWHKGVRAATSMIKKAVLLALGEAILAPEDLYGL
                     DDDSFYASLRAIKFAPFALAEAVFEGRLYPVVLDLPFDEGDERHRGLLDLGARLRLEG
                     EIAERASVGRTRLSPLDIVVDLPEPVSFETDLCVIMDRGRPGNGAGSSLGFAQSPTVF
                     SPPVVAGFARALRRVRVFCADSQAAVGKAAHELLGGGQARGVVAVSSGKA"
     gene            2428..3144
                     /locus_tag="VFL04_08505"
     CDS             2428..3144
                     /locus_tag="VFL04_08505"
                     /inference="COORDINATES: protein motif:HMM:NF022179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HET7839788.1"
                     /translation="MEIHNLMEYAVQAVVDELFDHETREPQLGYCTCDQCRLDVACYV
                     LNRVKPEYIVSSRGLAYSEKEGLDKVQRQADIITLVKEGWGRVTHAPRSTSDHTGPHA
                     ASQEMKGPAFNFPTVMGRVFDGRTFAPVPEGKVTLLEAGAQVNMVDPNWQNPFVLAGG
                     TAGTYIFWPCPLAAPGLGATRTWTFEIKVEAQGFDPLSHFVELQLEGEDEARTDFSLQ
                     RVHKIPDLFLFPEGTEEGGD"
BASE COUNT          483 a         1143 c         1107 g          487 t
ORIGIN      
        1 aggcgcaggg gcatggcgtg cgagatcgtg aggccgtcgc tggccatctc ggtgatgttg
       61 acgggccatt cgcccgtgcc cgcgatgagg aggagctgca ccttgtcatc ccggaccgag
      121 ctgaggacga attccttctc gatgcgtttg aggctcgtgg ccatcttcct agtctcctgg
      181 tccgagggcc gggggcatcc ccggctcgaa ggtcgagcca cgcgccttcg cggcgacggc
      241 gaacgaaaga ctctcgacgc gccagacttc cccgtccgcg cggaggctca ggaggccctc
      301 gtgggagggc ccatccccgg cgggggcaag cctgaccctc atccaggcct cgtcgcccga
      361 cagcgatatc tggccgtagc ggaaatcgcc cccctgcatc tctcccgaga gcggcgcgag
      421 ggcgagggag agggcctccc tctcctcggg gacgaggagg gcgggatcga gcttgccctt
      481 ggccaggccg tccatgagac cctgggccgt cgcgagcgcc gagctgagcc tgtccgatcc
      541 gctcctgtag tcctggaggg ccccgatcga gaagtcctcc ggcatgatgg ccgcgttggc
      601 cgacagcccg aaggccctga gcgatgcggc gaggacgggc ctcggcttgg gcttggccgg
      661 ctgcggcggg aggctcgggc tccccgtctt gggcgcctcc tgcgggcccg gcgccggggc
      721 tcgcgcgctt gcccccgcgg gggtctgggg ggcggcgagg aggggcgagg ggccctgggg
      781 cgcgggggtc tgagcggggg aggcaagggc tccggacgag gtctgcgctc gggaggccgg
      841 ggatgcgcgg gagcaggaca gggaggccga cagcaggagc cccgccgcga gcgcggcgac
      901 gaggctgatc ccgggcgaag ccacaggcgc gcggaatccc ggtgagctgt acaagctggc
      961 catctcgtgt aaatatgtag cgcaaacgtg gccatgggtc aatcgaagcg gaggcaggcg
     1021 atgaaatcgg gggcgctcct tgagcatctg gcgtcgtcct ttaccgaacc ggtgcgggac
     1081 cccctctggg gcaacatctt cctgagcccg gggctcgagg ccctcgcgaa ctccggcccc
     1141 ttcgccaagc tcgcgaggat aaaacagctc gggccggccc acctcgtcta ccccggggcg
     1201 acgcacacgc gccgcgccca ttccctcggg gtcttccaca tggccaggag gctctgccag
     1261 gccctggtcg cgcgcggggg catcgagttc gtcacgcgcg agggcctcga ctccttcctc
     1321 gccgcggcgc tctgccacga cctcgggcac ttccccttcg cccactccct caaggagctg
     1381 cccctggccg accacgaggc cctgacggcc cgcctcctcg tcgaggagcc cctgcgctcc
     1441 ctcgtgggca cgagcggagc cgaccccgag gcgacggcgg ccatcgtgga ccaggacctc
     1501 cccgaccgcg gcgacaggga gatacgcttc ttccgcggac tcctctccgg cgtcctcgac
     1561 cccgacaagc tcgactacct cacccgggac gccttcttct gcggcgtgcc ctatggcatc
     1621 caggactcgg acttcatcct ccagcgggtc gggatagccg aggaggacag gctcggcatc
     1681 gaggagaggg gcctcatgag cgtggagagc ctgctcttct cgaagtacct catgtaccgc
     1741 tcggtctact ggcacaaggg ggtcagggcc gccacctcca tgatcaagaa ggccgtcctc
     1801 ctcgccctcg gcgaggcgat actcgccccc gaggacctct acggcctcga cgacgactcc
     1861 ttctacgcca gcctgcgcgc aatcaagttc gccccctttg ccctggccga ggccgtcttc
     1921 gaaggccggc tctaccccgt tgtcctcgac ctgcccttcg atgagggcga tgagcgacac
     1981 aggggcctcc tcgacctcgg cgcaaggctc aggctcgagg gcgagatcgc cgagcgggcc
     2041 tcggtgggtc gaacgcgcct cagccccctc gacatcgtcg tcgacctgcc ggagcccgtc
     2101 tccttcgaga ccgacctctg cgtgatcatg gaccgcggca ggccgggcaa cggggccgga
     2161 tcgagcctgg gcttcgccca gagccccacg gtcttctccc cccccgtggt ggccggcttt
     2221 gcccgggccc tgcgccgggt cagggtgttc tgcgccgact cccaggccgc ggtggggaag
     2281 gcggcccacg agctccttgg cggcggccag gctcggggtg tcgttgcggt ttcgtcggga
     2341 aaagcctaga cttgcatggt ggcgggcgct ttgggccccg cccagcaggg cggcatggtc
     2401 cgcggcgcca agctttcagg aggctgcatg gaaatccaca atctcatgga gtacgcggtc
     2461 caggccgtcg tggacgagct cttcgaccac gagaccaggg agccccagct gggctactgc
     2521 acctgcgacc agtgccgcct cgatgtggcc tgctatgtcc tcaaccgggt gaagcccgaa
     2581 tacatagtct cgagccgggg cctcgcctac agcgagaagg aaggcctcga caaggtgcag
     2641 cggcaggccg acatcatcac cctcgtgaag gagggctggg ggagggtgac ccatgccccg
     2701 aggtccacct cagaccacac ggggccccat gcggcgagcc aggagatgaa gggccccgcc
     2761 ttcaacttcc cgacggtcat gggcagggtc ttcgacggcc ggaccttcgc ccccgtgccc
     2821 gagggcaagg tcaccctcct cgaggccggg gcccaggtga acatggtgga tccgaactgg
     2881 cagaacccct tcgtcctcgc gggcggcacc gcgggcacct acatcttctg gccctgcccc
     2941 ctggccgccc ccggccttgg cgcgacccgc acctggacct tcgagatcaa ggtcgaggcc
     3001 cagggcttcg accccctctc gcacttcgtc gagctccagc tcgagggaga ggatgaggcg
     3061 cgcaccgact tctccctcca gcgcgtgcac aagatccccg acctcttcct cttccccgag
     3121 ggcaccgagg agggcgggga ctaggcccac ggccccaccc cgggcgggcc ctagcgggtg
     3181 taggccacga gctcgactgc ggggacgagc tcgagatcgt
//
LOCUS       DASSQV010000483         5709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2102610, whole genome shotgun sequence.
ACCESSION   DASSQV010000483 DASSQV010000000
VERSION     DASSQV010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102610"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFL04_08510"
     CDS             complement(<1..441)
                     /locus_tag="VFL04_08510"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET7839789.1"
                     /translation="MRSIAAILASLVLAAGLSSCYVTSQASRYLAIRSRAVPVDRALA
                     DPRTPATLRALLDRAVAVRAFAESSIGLKRTRNFGSIVQLEGDHLASVVSACAELGFR
                     RYLWTYPLVGRLPYRGYFDQAQADAEAARLKKLGLDVIVRPVDAF"
     gene            578..1141
                     /locus_tag="VFL04_08515"
     CDS             578..1141
                     /locus_tag="VFL04_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839790.1"
                     /translation="MKIVRAVLTVLAVVLVLASCSSMAQGAVSGALNSLGSGGGLAPS
                     GGGAMAAGSGTASVDFQSGEVLSSDDTGKMMDAAYDLSKILTPASPATKNQAEAVFID
                     GGKKQWVNYVVNSRKASKSDFTVGATVFILAGWQGHDEISADSYRKGTWHLATITSVE
                     DLFKNRVEVGGNSFNINYIRIPTDPIR"
     gene            1247..1972
                     /locus_tag="VFL04_08520"
     CDS             1247..1972
                     /locus_tag="VFL04_08520"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7839791.1"
                     /translation="MDELGSRTEGGLVAADGHFFAELPQESRFYIVRHGQSEGNARMV
                     IQGRQDLPLDETGRRQAAAAAAWLCGKGISSISASPLSRAAETASIIAAACGLGAPSL
                     DPVFMEIDTGMFSGLSLDEAKEAHPEAYEAFRHLSWDGVSDAEPSPRIYERAVSAWEL
                     LRARALAGERAVACVTHGGFIQWLVRSTFGCRSWMPLLPTSNCGIFELLVEPTGRGRG
                     AYMHWNLLNFQSAAGLKAMPQVF"
     gene            complement(1982..2731)
                     /locus_tag="VFL04_08525"
     CDS             complement(1982..2731)
                     /locus_tag="VFL04_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HET7839792.1"
                     /translation="MDQFEILYEDEDLLVANKLAPLPVQKEKTKDPSLQELLSGSGSG
                     RHFLEAAHRIDRRASGAVLFAKTSKSLTTIEAAFRQRKVEKEYLACVEREPVPAEGKL
                     EHNLVWDRRRNIVRALPLGPGSQEVVASDRAVPSVGTGAGTPKGSKATETERALLEYR
                     LVGRSERYFFVAVDLITGRHHQIRAQFSASGFPIRGDLKYGAKRSCPNGLIMLHARKL
                     AFAQPRTGARIEVIAPFPASEPLWAVLAAED"
     gene            2987..3715
                     /locus_tag="VFL04_08530"
     CDS             2987..3715
                     /locus_tag="VFL04_08530"
                     /inference="COORDINATES: protein motif:HMM:NF023540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HET7839793.1"
                     /translation="MERVAAVFGRAEDGQSQAGPLAAGAIEPVGSAAAALPNEQAAAR
                     APGRAPGRDPRRPELLVGARAGEAQGRAEDAASAEKAALQRRQVERQGSQSLEVLHMI
                     ELLRSRDAEVRAHEAAHVAAGGSYITGGASYTTERGPDGRSYAVGGEVGIDTQPIPGR
                     PEETANKMRIVRAAALAPIDPSSADLAVAAAAAAMEAEAMAQAAAMRAEDAARSAQAV
                     RSYGGAAGGPGNRGGSEKRVDVVA"
     gene            3774..4481
                     /gene="deoD"
                     /locus_tag="VFL04_08535"
     CDS             3774..4481
                     /gene="deoD"
                     /locus_tag="VFL04_08535"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET7839794.1"
                     /translation="MSVHIAAAKGQIAEGILLPGDPLRAKFIAETFLEKPERFNEVRN
                     MFGYTGLYKGERVSVMGTGMGMPSLSIYVNELIKDYGVKRLIRVGTCGSMKKEVGVRE
                     LILAMSACTDSAVNRMRFGGMDFAATASFRLLLAAHQAAQAAGAKAHVGSILSTDSFY
                     QHDPELWKLWASFGVMAAEMESAELYTLAAKYSVEALAILTVSDHIVTGEETTSAERE
                     RSFKAMVELGLTALLAK"
     gene            complement(4634..5122)
                     /locus_tag="VFL04_08540"
     CDS             complement(4634..5122)
                     /locus_tag="VFL04_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7839795.1"
                     /translation="MRAVTWKKGIRFEREASFRLEAPKTAVFPLLCPVLEEKWLPLWS
                     CTMVYSESGVAERDAVFTTREGHFMKATWTLITHEPDELVEYLIVGSKGMIVRLSIAL
                     RAVDGDRGTEVTWTMRFTLSSKAGAAIAHHGFGEAKFADMIERRRLQLAHYLRTGTML
                     EA"
     gene            complement(5180..>5709)
                     /locus_tag="VFL04_08545"
     CDS             complement(5180..>5709)
                     /locus_tag="VFL04_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7839796.1"
                     /translation="AVYTIGRLGELVGLSRSTLLYYDSIGLLRPSGRSGAGYRLYTED
                     DRRRLERIVALRRTGLALERIASCLEGGEDGPAGMLLRRVFDINGEIEGLRAQQKALL
                     DLIEADGRLKDSRRILHGLEALGREAGVDRDSYLGIHQAFERSSPEAHRRLLGFLGFA
                     EPDIEDFLSTLNDSL"
BASE COUNT          904 a         1975 c         1998 g          832 t
ORIGIN      
        1 gaaggcgtcc accggcctca cgatgacgtc gagcccgagc ttcttgagcc gcgccgcctc
       61 ggcatccgcc tgggcctggt cgaagtagcc ccggtagggc agccggccga cgaggggata
      121 ggtccagaga tagcggcgga agcccagctc ggcgcaggcc gagaccaccg aggcgaggtg
      181 gtcgccctcg agctggacga tcgagccgaa gttgcgcgtc cgtttgagcc cgatgctgct
      241 ctcggcgaag gcgcggacgg ctaccgcccg atcgaggagg gcgcgcagcg tggccggggt
      301 tcgggggtcg gcgagggccc gatcgaccgg cactgcccgg gagcggatgg cgaggtagcg
      361 ggaagcctgg ctggtgacat agcaggagga gaggcccgcc gcgaggacca gggaggcgag
      421 gatggccgcg atgctgcgca cggtacgact ctagccccgg ggatcgcggc atggcaagcg
      481 ccgacggcgg tccggggccg ccccggcccg atgcggcttg attttcccgg gttgggcgat
      541 agaatggggg cagaattttt caaagaagga gctcaccatg aaaatcgtcc gcgctgtcct
      601 caccgtcctg gcggttgtcc tcgtcctcgc ctcatgctcg tccatggccc agggggccgt
      661 ctcgggcgcc ctcaattccc tcggctctgg cggcggtctg gccccctcgg gcggcggcgc
      721 gatggctgcc ggaagcggca cggcgagcgt cgacttccag tccggcgagg tcctctcctc
      781 ggacgacacc ggcaagatga tggacgccgc ctacgacctg tcgaagatcc tcaccccggc
      841 cagccccgcc acgaagaacc aggccgaggc cgtgttcatc gacggcggca agaagcagtg
      901 ggtgaactac gtcgtcaaca gccgcaaggc cagcaagtcc gacttcacgg tgggcgccac
      961 cgtcttcatc ctcgccggct ggcagggcca cgacgagatc tccgcggact cctaccgcaa
     1021 gggcacctgg cacctcgcca ccatcacctc cgtcgaggat ctcttcaaga accgcgtcga
     1081 ggtcggcggc aactccttca acatcaacta catccgcatc cccaccgatc ccatccgcta
     1141 aggcccgcgg cgcgccccat gggcgcgcgg cagccaatcc acacgaaggc ccgccacccg
     1201 gcgggccttt gccattggcg gccaagagcc ggtatactcg cccaccatgg atgagctggg
     1261 ttcgaggacc gagggcggct tggtcgccgc cgacggccat ttcttcgcgg agctgcccca
     1321 ggagagccgc ttctacatcg tccgccacgg gcagagcgag ggcaacgcga ggatggtcat
     1381 ccaggggaga caggacctcc ccctggacga gacgggcagg aggcaggccg ccgcagccgc
     1441 ggcctggctc tgcggcaagg gcatctcctc gatcagcgcc tctcccctct cgcgggcggc
     1501 cgagacggcg agcatcatcg ccgcggcctg cggcctcggg gctcccagcc tcgatccggt
     1561 gttcatggag atagacacgg gcatgttctc gggcctgagc ctggacgagg cgaaggaggc
     1621 ccatcccgag gcctacgagg ccttcaggca tctgagctgg gacggggtct cagacgccga
     1681 gcccagcccc aggatctacg aaagggccgt ctcggcctgg gagctgctcc gcgcccgcgc
     1741 cctggcgggc gagcgggctg tggcctgcgt aacccacggc ggcttcatcc agtggctcgt
     1801 gcgctcgacc ttcggatgcc gctcatggat gcccctcctg ccgaccagca actgcgggat
     1861 cttcgagctc ctcgttgagc ccaccggaag ggggagggga gcctacatgc actggaacct
     1921 gctcaacttc cagagcgcgg ccgggctcaa ggccatgccc caggtcttct aggaggggcg
     1981 gctagtcctc ggccgccagg acggcccaga gcggctccga ggccgggaag ggggcgatca
     2041 cctcgatcct tgccccggtc cggggctggg cgaaggcgag cttcctcgcg tggagcatga
     2101 tgaggccgtt ggggcagctc cgtttggccc cgtacttgag gtccccgcgg atggggaagc
     2161 ccgaggccga gaactgggcc cttatctggt ggtgcctgcc cgtgatgagg tcgacggcga
     2221 cgaagaagta gcgctccgag cggccaacga gccggtactc aaggagggcg cgctcggtct
     2281 ccgtggcctt cgaccccttt ggggtcccgg ctccggtccc gaccgagggc acggccctgt
     2341 ccgaggccac gacctcctgg ctcccaggtc caagggggag ggccctgacg atgttcctgc
     2401 ggcggtccca gaccaggttg tgctcgagct tcccctcggc ggggacgggt tcgcgctcga
     2461 cgcaggccag gtactccttc tcgaccttgc gctgcctgaa ggcggcctcg atcgtcgtga
     2521 gcgacttgga ggtcttcgcg aaaagcacgg cccccgaggc gcggcggtcg atgcggtggg
     2581 ctgcctcaag gaagtgccgc cccgagcccg agccgctcag cagctcctgg agggaggggt
     2641 ccttggtctt ctccttctgg accggcaggg gcgcgagctt gttcgcgacc agcaggtcct
     2701 cgtcctcata gagtatctcg aattggtcca ccgcagcctc cgaagggcat gacgatagca
     2761 cggagggcgg gcccggggat agcgcggggc cgcgcgcggc caatgcctgg cgcccagttc
     2821 gggcgaaaag cccggggcac caacaggctt cgaggcagcg tcttcgcttg caggctgggc
     2881 ccggcgtggc tatctttccc ttatggcgat ccccgccgtc tcttcgatcg ggggtgggtc
     2941 ctcctccttc cccgcccgag cgggagagcc aggcaggatc gccccggtgg agcgggtggc
     3001 cgccgtgttc ggccgggccg aggacgggca gtcccaggcc gggccgctgg ccgcgggggc
     3061 catcgagccc gtcgggtcgg ccgctgctgc cctcccgaac gagcaggccg cggcccgggc
     3121 tccgggcagg gcccctggaa gggatccgcg cagacccgag ctgctcgtgg gtgcgagggc
     3181 gggagaggcc caaggccgcg ccgaagacgc ggccagcgcc gagaaggcgg ctcttcagcg
     3241 ccgccaggtc gagcgccagg gctcccaatc cctcgaagtc ctccacatga tcgagctcct
     3301 caggtcgagg gacgccgagg tgcgcgccca cgaggccgcc cacgtcgccg ccggcggcag
     3361 ctacatcacg ggcggggcga gctacaccac ggagcgcggc cccgacggcc gcagctacgc
     3421 cgtgggcggg gaggtcggca tcgacaccca gcccataccc ggcaggcccg aggagacggc
     3481 caacaagatg aggatcgtgc gcgcggccgc ccttgccccc atcgacccct cgtcggccga
     3541 cctcgccgtc gcggccgcgg ccgcggcgat ggaggccgag gccatggccc aggccgcggc
     3601 catgcgcgcc gaagacgccg cgagaagcgc ccaagcggtg cgcagctacg gcggcgcggc
     3661 cgggggcccc ggaaatcggg gcggttccga aaagcgggtg gacgtggtag catgatgccc
     3721 cggcgcggct cggcccgcgc acgatcgcgt tcctacagcg aaagaaggtg cacatgagcg
     3781 tccacattgc cgcggccaag ggccagatcg cggaaggcat cctcctcccc ggagaccccc
     3841 tccgggccaa gttcatcgcc gagacctttc tcgagaaacc cgagcgtttc aacgaggtcc
     3901 gcaacatgtt cggctacacg ggcctgtaca agggcgagcg cgtctccgtc atggggacgg
     3961 gcatgggcat gccgagcctc tcgatctatg tcaacgagct catcaaggac tacggggtca
     4021 agcgcctcat cagggtcggg acctgcggct ccatgaagaa ggaggtcggg gtgcgcgagc
     4081 tcatcctggc gatgagcgcc tgcaccgatt ccgccgtcaa ccggatgcgc ttcggcggga
     4141 tggacttcgc ggccaccgcc tcattcaggc tcctcctcgc cgcccaccag gccgcccagg
     4201 ccgctggcgc gaaggcccat gtcgggtcca tcctctccac cgacagcttc taccagcacg
     4261 atcccgagct ctggaagctc tgggcctcat tcggcgtcat ggcggccgag atggagagcg
     4321 ccgagctcta caccctcgcc gccaagtact ccgtcgaggc cctcgccatc ctcacggtga
     4381 gcgaccacat cgtcaccggg gaggagacga cgagcgccga gcgcgagcgg agcttcaagg
     4441 ccatggtcga gctgggcctc actgccctgc tggccaagta gggcagtcgc cgccctcttg
     4501 tccaaatagg gcggctgccc tgctcgccaa gtagggcggc cgccgagatt gaggcgggcc
     4561 tggttggacg ggccggagcc tcggggcagg ccatccatgg ccggcgcccc ggggctctga
     4621 cgcccgcccg ccctcaggcc tccagcatcg tccccgtcct caggtagtgg gcgagctgga
     4681 ggcggcgtcg ctcgatcatg tcggcgaact tggcctcgcc gaagccgtga tgcgcgatcg
     4741 ccgccccggc cttggacgag agggtgaagc gcatggtcca ggtgacctcc gtgcccctgt
     4801 ccccatccac agcgcgcaga gcgatggaga gccgtacgat catgcccttt gagcccacga
     4861 tgaggtactc gaccagctcg tcgggctcat gggtgatgag ggtccaggtc gccttcatga
     4921 aatggccctc cctggtggtg aagacggcgt ccctctcggc gaccccgctc tccgagtaga
     4981 ccatcgtgca ggaccagagc ggcagccact tctcctccag cacggggcag agcaggggga
     5041 agaccgccgt cttcggcgcc tcgagcctga agctcgcctc cctctcgaac ctgattccct
     5101 tcttccacgt cacagcgcgc acatcgtcct ccttcggatc ggcgatgcgt cgaagctaga
     5161 ctgtgaagcc gtagacgggt caaagggaat cgttcagggt cgagaggaaa tcctcgatgt
     5221 cgggctcggc gaagccgagg aagccaagga ggcggcggtg ggcctcgggg gagctgcgct
     5281 cgaaggcctg gtggatgccc aggtagctgt cccggtcgac ccccgcctcg cgcccgaggg
     5341 cctcgagccc gtgcaggatc cgcctcgagt ccttgagccg gccatcggcc tcgatcaggt
     5401 cgaggagggc cttctgctgg gccctgaggc cctcgatctc gccgttgatg tcgaagaccc
     5461 ggcgcaggag catcccggcg gggccatcct cgccgccctc gaggcagctg gcgatgcgct
     5521 cgagggccag gcccgtccgc cgcagcgcga cgatccgctc gaggcggcgt cggtcgtcct
     5581 cggtgtagag gcggtagccg gccccgctcc tccccgaggg tcggagcagg ccgatcgagt
     5641 cgtaatagag gagggtggag cgcgacaggc cgacgagttc gcccaacctg ccgatcgtgt
     5701 acacggccg
//
LOCUS       DASSQV010000484         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_984529, whole genome shotgun sequence.
ACCESSION   DASSQV010000484 DASSQV010000000
VERSION     DASSQV010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984529"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="VFL04_08550"
     CDS             <1..1569
                     /locus_tag="VFL04_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HET7839797.1"
                     /translation="GADHSPMGVLNFSLFDKRRPGCRVKQAGRGGIGTVLRDKKIRAI
                     VGRYRGVEQNSNDAADPAAVQRIGLKLHREIIVNDDKQCKMRKQGTAHLMEVMNDYDL
                     LPTKNHKFGQDPRGPEIASWVWEKLFSQDLPDGCWFGCTMACAHAVDKFELETGPYKG
                     QKVCVDGPEYETAAGCGSNLYVWDPHGILEINFYCDTYGIDTISVGTTCGFLMECWEL
                     GILNAERTGGVDLSWGNWKGVCELMHQLAEGRGFGLLMGKGVKFLAEHFTKEFGADAQ
                     LMKDIALHGKGLEQSEYISKESLAQQGGYYLTNKGPQHDEAWVIFMDMVNNRIPTFEN
                     KAEALHYFPMFRTWFGLQGLCKLPWNDVEPADNKRWTEPNKVPEHVDNYRALYTAITG
                     ESLDWDGMIRMSEKVYNFQRVFNLRLGHGKRVDDYPPYRAMGPVTEEEYLSRAERYDK
                     QLKEKVGLDPAGKTTAQKIAALRKYREAQYESLIDAVFSRRGWTRDGVPTPEHLRKIG
                     MDIPELMAVVEKHL"
     gene            1757..2212
                     /locus_tag="VFL04_08555"
     CDS             1757..2212
                     /locus_tag="VFL04_08555"
                     /inference="COORDINATES: protein motif:HMM:NF015317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HET7839798.1"
                     /translation="MVLVTVVASLVLAAAAILWGAYRCAVVLAGIVTDPHQASSVAFL
                     KLMDIFLIGVALLVFSVGLYRLFIGELSLPEGLLADSLHELESKLGSLVILVMAIHLL
                     EQFIEWGEPRSIVSMALVFVLASAAIIANNVLVNRGKDQPRDPGREARG"
     gene            complement(2209..2745)
                     /locus_tag="VFL04_08560"
     CDS             complement(2209..2745)
                     /locus_tag="VFL04_08560"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HET7839799.1"
                     /translation="MALKATVFKAKLQVADLDRHYYAEHRLTLARASSETDERLMVRI
                     IAFALNAGEDLLLTSEMAETEEPSLWRKDPGGRIRLWIEVGLPEPRRLKKASGRADEV
                     LLYLYHGRQARLWWEANRAELEAIRGLAVVELAQDSVRELAALAERSMDLQVTIQDAQ
                     ASFTHEGGILELGLTRLL"
     gene            complement(2761..>3570)
                     /locus_tag="VFL04_08565"
     CDS             complement(2761..>3570)
                     /locus_tag="VFL04_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HET7839800.1"
                     /translation="FSAVDGPGNRGVVFLQGCDFDCSYCHNPETRMPCSACGACIGVC
                     PETALSGGPVSRPVWDRELCRDCGSCVAACRLGSSPKVRRLGAGAVLDELEPYRPFLR
                     GVTVSGGECLLQPEFLSELLELARSRGLPGLVDTNGSRPLASLPRILGAAEGFMLDVK
                     AWNEAEHWALTGSTNAGVLENLRFLAGRDALYEVRTVLAPGSFDLEETVRETAAVLAA
                     TRSRARYRLIRYRPFGVRADRAAILHEPDEALMERLSALALAAGAATVLVS"
BASE COUNT          612 a         1224 c         1207 g          527 t
ORIGIN      
        1 ggcgccgacc acagccccat gggcgtcctc aacttctcgc tcttcgacaa gcgccgcccg
       61 ggctgccgcg tgaagcaggc gggccgcggc ggcatcggca ccgtcctgcg ggacaagaaa
      121 atccgcgcca tcgtgggccg ctaccgcggg gtcgagcaga actccaacga cgccgccgac
      181 ccggccgccg tgcagcgcat cggcctcaag ctgcaccgcg agatcatcgt caacgacgac
      241 aagcagtgca agatgcgcaa gcagggcacg gcccacctca tggaggtcat gaacgactac
      301 gacctcctgc ccacgaagaa ccacaagttc ggccaggacc cccgcggccc cgagatcgcc
      361 tcgtgggtct gggagaagct cttctcccag gacctgcccg acggctgctg gttcggctgc
      421 accatggcct gcgcccacgc cgtggacaag ttcgagctcg agaccgggcc ctacaagggc
      481 cagaaggtct gcgtcgacgg ccccgagtac gagaccgccg ctggctgcgg ctcgaacctc
      541 tacgtctggg atccccacgg catcctcgag atcaacttct actgcgacac ctacggcatc
      601 gacacgatct ccgtgggcac cacctgcggc ttcctcatgg agtgctggga gctcggcatc
      661 ctgaacgcgg agcgcaccgg tggcgtcgac ctcagctggg gcaactggaa gggcgtctgc
      721 gagctcatgc accagctcgc cgagggccgc ggcttcggcc tcctcatggg caagggcgtg
      781 aagttcctgg ccgagcactt caccaaggag ttcggcgccg acgcccagct catgaaggac
      841 atcgccctgc acgggaaggg cctcgagcag agcgagtaca tctcgaagga gtccctcgcc
      901 cagcagggcg gctactacct cacgaacaag ggcccccagc acgacgaggc ctgggtcatc
      961 ttcatggaca tggtgaacaa ccgcatcccg accttcgaga acaaggccga ggccctgcac
     1021 tacttcccca tgttccgcac ctggttcggc ctccagggcc tctgcaagct gccctggaac
     1081 gacgtggagc ccgccgacaa caagaggtgg accgagccca acaaggtacc cgagcacgtc
     1141 gacaactacc gcgccctcta cacggccatc accggggaga gcctggactg ggacgggatg
     1201 atccgcatga gcgagaaggt ctacaacttc cagcgggtct tcaacctccg cctcggccac
     1261 ggcaagcgcg ttgacgacta cccgccctac agggccatgg ggccggtgac cgaggaggag
     1321 tacctctcac gggccgagcg ctacgacaag cagctcaagg agaaggtcgg cctcgacccc
     1381 gcgggcaaga cgacggccca gaagatcgcc gcgctgagga aataccgcga ggcccaatac
     1441 gagagcctca tcgacgccgt cttcagccgc cgcggctgga ccagggacgg agtgcccacg
     1501 cccgagcacc tcaggaagat cggcatggac atccccgagc tcatggccgt cgtcgagaag
     1561 cacctctaga ccagaccaac acaggatcga gcgggcggcc cccctggggc cgcctttttt
     1621 tgcgccggcc tggccgtggg agcgtggagc ttgccgggca tcgggggcca gggttatttt
     1681 ctcctcgggc tcccggcgcc acgccggggg ggcggggagg cgggcggtgc gcagaatcct
     1741 cgaggccagc aagtatatgg ttctggtgac ggtggtcgct tccctcgtgc ttgcggccgc
     1801 cgcaatcctg tggggagcct ataggtgcgc cgttgtgctg gccggaatcg tgacggatcc
     1861 ccaccaggcc agctcggtcg ccttcctgaa gctcatggac atcttcctga tcggcgtcgc
     1921 cctgctcgtg ttctcggtcg gcctctatcg cctcttcatc ggcgagctct ccctaccgga
     1981 gggcttgctg gccgacagcc tccacgagct ggagtccaag ctggggagcc tcgtgatcct
     2041 ggtgatggcc atccacctgc tggagcagtt catcgaatgg ggggaaccgc ggtcgatcgt
     2101 ctcgatggcc ctggtcttcg ttcttgcctc ggccgccatc atcgcgaaca acgtcctcgt
     2161 gaaccgaggc aaggaccagc cgcgggaccc gggccgcgag gcccgcggct agagcagcct
     2221 cgtcagcccc agctccagga tccctccctc atgggtgaag gaagcctgag catcctggat
     2281 ggtcacctgg agatccatgc tcctctcggc cagggcggcg agctcgcgca ccgagtcctg
     2341 ggcgagctcg accacggcga ggccgcgtat ggcctcgagc tcggcgcggt tggcctccca
     2401 ccagagccgg gcctggcggc cgtggtagag atagaggagg acctcgtcgg cccggcccga
     2461 ggccttcttg agccgtctgg gctcggggag gcccacctcg atccagagcc tgatccgccc
     2521 gccgggatcc ttcctccaga ggctgggctc ctcggtctcg gccatctcgc tcgtgaggag
     2581 gagatcctcc ccggcgttga gggcgaaggc gatgatcctc accatcaggc gctcgtccgt
     2641 ctcggagctg gcccgggcga gggtgagcct atgctcggcg tagtagtgac ggtcgaggtc
     2701 ggcgacctgg agcttagcct tgaagacggt ggccttgagc gccatgcgga tagctccttt
     2761 ctaggacacg aggaccgtgg cagccccggc agcgagggcc agggccgaca gacgctccat
     2821 gagggcctcg tcgggctcgt ggaggatggc ggcgcggtcg gccctgaccc cgaagggcct
     2881 gtagcggatg agcctgtacc gggccctcga gcgcgtcgcg gcgaggacgg cagccgtctc
     2941 cctcaccgtc tcctcgaggt cgaaggagcc gggcgcgagc accgtcctga cctcatagag
     3001 ggcgtcgcgc ccggcgagga agcgcaggtt ctcgaggacg cccgcgttgg tcgagcccgt
     3061 gagcgcccag tgctcggctt cgttccaggc cttcacgtcg agcatgaagc cctcggcggc
     3121 cccgaggatc cggggcaggg aggcgagggg cctcgaaccg ttcgtgtcga cgaggccggg
     3181 caggccgcgc gagcgcgcaa gctcgagtag ctccgaaagg aactcgggct ggaggaggca
     3241 ttccccgccc gagaccgtga cgccgcgcag gaagggcctg tagggctcga gctcgtcgag
     3301 gacggccccg gcgccgagcc gcctgacctt gggggagctc cccagtcggc aggccgccac
     3361 gcaggagccg cagtcgcggc agagctccct gtcccagacc ggccgcgaaa ccggtccgcc
     3421 ggagagggcc gtctcggggc agaccccgat gcaggcgccg caggccgagc agggcatccg
     3481 ggtctcgggg ttgtggcagt aggagcagtc gaagtcgcag ccctggagga agacgacgcc
     3541 gcggttgccc ggcccgtcca cggcgctgaa
//
LOCUS       DASSQV010000485         5788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1605390, whole genome shotgun sequence.
ACCESSION   DASSQV010000485 DASSQV010000000
VERSION     DASSQV010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605390"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2734
                     /locus_tag="VFL04_08570"
     CDS             <1..2734
                     /locus_tag="VFL04_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7839801.1"
                     /translation="YIRPSFEELERAEARQDLDRVIAAVDREAEHLATTVRDWSQWDD
                     TYDYVGGLNPDFVDDLGPDGFHTLKADLVAIYGPDSVLRFSGRLDRGSGTVIPDPDFP
                     KRAPPSAPYLVKTTEASGISGLLSRTGGCYLIASGPILRNDGSGSLRGSLIMGRIVDD
                     AFSASIAAQTGVRAVLGSPQDLPKGIDLATIAAHGMRYARAAKGEPDVHGYGLISDIS
                     GQPILLVRTETARAIGAWTERTIRISGAMLLVIGGAICLLIYAMLLRLVISPLSTIGE
                     RLGGSQAAPSSLPLGLLERRDEIGRLAQTLQQMAAALGVRDRALRMANERLETRIEER
                     TRELRKASDELRLMGKVVESTSEAIVITDLGGNILLVNEAFCRQSGYRPEEVIGRNPR
                     LLKSGRHGVQYFRELWDRLAAEGSWAGEIWNRTKGGDIHPEWLTINLIRGEDGEPRCY
                     VGVSTDISLMKDTEDKLNRLAYYDPLTGLPNRALFKDRLERAILQAERYGHRVALLMI
                     DLDRFKFVNDALGHEAGDRLLIEVARRIAQRVRASDTVCRLGGDEFTVILDRIGRSEH
                     ASIVARGIITELGSPFALKEREVFIGASIGISVYPSDDEASEGLTRKADAAMYQAKAA
                     GRNTYRFASGETEATSQTRIALESELRRAVERGEFHLVFQPIVDLAGNRVLGAEALVR
                     WRRGDGKLELPGDFIPLAEETGIIVQLGDWILRAACSAAAAWRDLGLPMYVSVNISPR
                     QFEQPGLALRIETALAEAGLEPELLAVEITESAIMRDVELALHTMSALKELGIRLAID
                     DFGTGYSSLSYLSRFPIDKLKIDQSFTRGIEESASTASIVNAVIAMAESLGIGTIAEG
                     IETEGQRDYLERRGCGEGQGYLFSEPIDAGAFAALGLRALRAVPPREGACGQSS"
     gene            complement(2731..5577)
                     /gene="uvrA"
                     /locus_tag="VFL04_08575"
     CDS             complement(2731..5577)
                     /gene="uvrA"
                     /locus_tag="VFL04_08575"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7839802.1"
                     /translation="MDKLIIKGAREHNLKNIDLELPRDKLIVISGLSGSGKSSPAFDT
                     LFAEGQRRYVESLSAYARQFLGRMDKPDVDYIEGLSPAISIEQKTTHRNPRSTVGTVT
                     EIYDYYRLLFARVGVPHCPQCGREIREQGIDQIIDTILSWPSGSRLQVLAPVIRAQKG
                     EHKKILEDATKQGFVRARVDGEIRELPSGGPIDFKLEKQRKHSIEIVVDRLRLSDDLR
                     RRLAESVETALQIADGTVILLVDEDPPREEFFSQNGACPECGISLPEMEPRLFSFNAP
                     QGACPTCSGLGVKLEFDPDLVIPDPSLSFNEGGCIPYNPDAAWNRSRFEALAKHFKFS
                     LDTPFQDLPKKVLDAILRGTDEAVKIKYENREGTGRFEYESRFPGILADLKRRYLETQ
                     SDGIKTWLERFMSEKACEACGGKRLRPEALAVTIGGISIHELSAKSVEATLEHFTKLA
                     LTETERQIAKQILKEITARLSFMRNVGLEYLTLERKAGTLSGGEAQRIRLATQIGSSL
                     VGVLYILDEPSIGLHQRDNQRLIDTLTYLRDIGNTLVVVEHDEQTLRTADWIVDLGPG
                     AGVHGGHVVAQGTPDQVMKSRRSITGQYLAGSLSIEVPPERRVPREESILIRGCREHN
                     LKGIDVRLPLGVFTCITGVSGSGKSTLLTDLLYPIISNKVMGTGWREGDYDSIEGLEA
                     IDKIINIDQSPIGRTPRSNPATYVGVFTQIRDLFASLPESKTRGWKPGRFSFNVKGGR
                     CENCQGDGTIKIEMNFLPDVYITCDVCHGRRFNSDTLDARYKGKNIADVLDMTIEEAA
                     GFFEHVPQIAHRMNTLLSVGLGYIKLGQSALTLSGGEAQRVKLAFELARRGTGRTLYF
                     MDEPTTGLHFADVKQLMEVIQRLVDAGNTVVMIEHNLDVIKQADWIFDLGPEGGDKGG
                     TILVEGTPEAVVKCRASYTGKYLKGVLQPPAVKA"
BASE COUNT          903 a         1928 c         2016 g          941 t
ORIGIN      
        1 ctacatcagg cccagcttcg aggagctgga gcgggccgag gccaggcagg acctggacag
       61 ggtcatcgcc gccgtggaca gggaggccga gcacctcgcg accacggtca gggactggag
      121 ccagtgggac gacacctacg actacgtggg gggcctcaat ccggacttcg tggatgacct
      181 cgggcccgac ggcttccata ccctcaaggc cgacctggtc gccatctacg gcccggactc
      241 ggtactgcgg ttttcgggcc gcctcgaccg cgggagcggc accgtcatcc ccgaccccga
      301 tttcccgaaa cgggcgccgc cttcggcgcc ctatctcgtg aagacgaccg aagcctcggg
      361 catcagcggc ctcctgtccc gcacgggggg atgctacctc atcgcctccg ggccgatcct
      421 gaggaacgac ggctcgggca gcctgcgcgg cagcctcatc atgggcagga tcgtcgacga
      481 tgccttctcg gcctcgatcg ccgcccagac gggggtgcgg gccgtcctcg gctcgcccca
      541 ggacctgccc aagggcatcg acctggccac gatcgccgcc catggcatgc gctacgcgag
      601 ggccgcgaag ggcgagcccg acgtccacgg ctacggcctc atctccgaca tctccggcca
      661 gccgatcctc ctcgtcagga ccgagacggc ccgggcgatc ggagcctgga cggaaaggac
      721 gatccggatc tcgggggcca tgctcctggt catcggcggg gcgatctgcc tcctcatcta
      781 cgccatgctc ctgcgcctcg tcatctcccc cctctcgacg atcggggagc gtctgggcgg
      841 cagccaggcc gcgcccagct ccctgcccct gggcctcctg gagcgcaggg acgagatcgg
      901 ccgcctcgcc cagaccctcc agcagatggc cgcggccctc ggcgtccgcg accgggccct
      961 gcgcatggcc aacgagaggc tcgagaccag gatcgaggag aggacccgcg agctccggaa
     1021 ggccagcgac gagctcaggc tcatgggcaa ggtcgtggag agcacgagcg aggccatcgt
     1081 gatcaccgac ctcggcggga acatcctcct cgtgaacgag gccttctgcc gccagagcgg
     1141 ctaccggccc gaggaggtca tcgggaggaa cccgcgcctc ctcaagtcgg gccgccacgg
     1201 cgtccagtac ttccgcgagc tctgggaccg cctcgccgcc gagggcagct gggccgggga
     1261 gatctggaac cggaccaagg gcggcgacat ccaccctgag tggctgacga taaacctcat
     1321 ccgcggcgaa gacggcgagc cccgctgcta cgtcggcgtc tccaccgaca tctcgctcat
     1381 gaaggacacc gaggacaagc tcaaccgcct cgcctactac gaccccctga cggggctgcc
     1441 caaccgcgcc ctgttcaagg acaggctcga gcgcgcgatc ctccaggccg agcgctacgg
     1501 ccaccgcgtc gccctcctca tgatcgatct cgaccgcttc aagttcgtca acgacgccct
     1561 cggccacgag gcaggcgacc gcctcctcat cgaggtggcg aggcggatcg cccagagggt
     1621 gcgggcctcg gacacggtgt gcaggctcgg cggcgacgag ttcaccgtca tcctggacag
     1681 gatcggcagg agcgagcacg cgagcatcgt ggcccgcggc atcatcaccg agctcggttc
     1741 ccctttcgcc ctcaaggagc gcgaggtctt catcggcgcg agcatcggga tctcggtcta
     1801 ccccagcgac gacgaggctt ccgagggcct gaccaggaag gccgacgccg cgatgtacca
     1861 ggccaaggcc gcgggccgca acacctaccg tttcgcctcg ggggagaccg aggccacgag
     1921 ccagacgagg atcgcccttg agtccgagct caggcgggct gtggagcggg gcgagttcca
     1981 cctcgtgttc cagcccatcg tcgaccttgc cgggaaccgc gtcctcgggg ccgaggccct
     2041 cgtgcgctgg cgcaggggcg acgggaagct cgagctgccg ggggacttca tccccctggc
     2101 cgaggagacc gggatcatcg tccagctcgg cgactggatc ctccgcgccg catgctcggc
     2161 agcggcggcc tggagggact taggcctgcc gatgtacgtc tccgtcaaca tttcgccccg
     2221 ccagttcgag cagcccggcc tcgccctcag gatcgagacc gccctggccg aggccgggct
     2281 cgagcccgag ctgctcgcgg tcgagatcac cgaatcggcc atcatgcgcg acgtcgagct
     2341 ggccctccac acgatgagcg cgctcaagga gctgggcatc aggctcgcga tagacgactt
     2401 cggcacaggc tactcgtccc tgagctacct gagccgcttc ccgatcgaca agctgaagat
     2461 cgaccagagc ttcacccgcg ggatcgagga gagcgcgagc acagcctcga tcgtcaacgc
     2521 cgtcatcgcg atggccgaaa gcctgggcat cggcaccatc gccgagggca tcgagaccga
     2581 gggccagagg gattacctgg agcgcagggg ctgcggggag ggccagggct acctgttcag
     2641 cgagcccatc gacgccgggg ccttcgccgc cctcggcctg cgggccctac gcgccgtccc
     2701 gccccgggag ggcgcctgtg gccagagctc ctaggccttg accgcgggcg gctggaggac
     2761 gcccttgaga tacttgcccg tgtaggaggc cctgcacttc acgacggcct cgggcgtccc
     2821 ctcaacgagg atcgtgcccc ccttgtcccc gccctcgggg ccgaggtcga agatccagtc
     2881 ggcctgcttt atgacgtcga ggttgtgctc gatcatgacc acggtgttgc cggcgtccac
     2941 cagcctctgg atcacctcca tgagctgctt cacgtcggcg aagtggaggc ccgtcgtcgg
     3001 ctcgtccatg aagtagaggg ttctgcccgt gccccgcctc gcgagctcga aggcgagctt
     3061 gacgcgctgg gcctcgccgc ccgagagggt aagggccgac tggccgagct tgatgtagcc
     3121 gaggcccacc gagagcaggg tgttcatgcg gtgggcgatc tggggaacgt gctcgaagaa
     3181 gccggcagcc tcctcgatcg tcatgtcgag gacgtcggcg atgttcttgc ccttgtagcg
     3241 ggcgtcgagg gtgtcggagt tgaagcgcct gccgtggcag acgtcgcagg ttatgtagac
     3301 gtcgggcagg aagttcatct cgatcttgat ggtcccgtcg ccctggcagt tctcgcagcg
     3361 cccgcccttg acgttgaagg agaagcgccc gggcttccag cccctggtct tggactcggg
     3421 cagcgaggcg aagaggtcgc ggatctgggt gaagacgccg acgtaggtgg cggggttgga
     3481 gcgcggagtc ctgccgatcg ggctctggtc tatgttgatg atcttgtcga tggcctccag
     3541 cccctcgatg gagtcgtagt ctccctccct ccaacccgtg cccatcacct tgttggagat
     3601 gatcggatag aggaggtcgg tgaggagggt ggacttgccc gagcccgaga ccccggtgat
     3661 gcaggtgaag acgcccaggg gcagcctgac gtcgatcccc ttgaggttgt gctcgcggca
     3721 gccgcggatg aggatcgact cctcgcgggg cacccgccgc tcggggggga cctcgatgct
     3781 gaggctgccg gcgaggtact ggccggtgat gctcctgcgg ctcttcatga cctggtcggg
     3841 cgtgccctgg gcgacgacat ggccgccgtg cacgccggcg ccgggcccca ggtcgacgat
     3901 ccagtcggcg gtgcgcaggg tctgctcgtc gtgctcgacg accacgaggg tgttgccgat
     3961 gtcgcgcagg taggtgaggg tgtcgatgag gcgctggttg tcccgctggt ggaggccgat
     4021 cgagggctcg tcgaggatgt agagcacccc gaccagggag gagccgatct gggtggccag
     4081 gcggatgcgc tgggcctccc ctcccgagag ggtgcccgcc ttgcgctcga gggtcagata
     4141 ctcgaggccg acgttgcgca tgaaggagag gcgggccgtg atctccttga ggatctgctt
     4201 cgcgatctgc cgctcggtct cggtcagggc tagcttcgtg aagtgctcga gggtggcctc
     4261 cacggacttg gcggagagct cgtggatgct gatgcccccg atcgtgacgg cgagggcctc
     4321 ggggcgcaga cgcttgccgc cgcaggcctc gcaggccttc tccgacatga agcgctcgag
     4381 ccaggtcttg atcccgtcgc tctgggtctc gaggtagcgg cgcttgaggt cggcgaggat
     4441 gccggggaag cgcgactcgt actcgaagcg gcccgtgccc tcgcggttct cgtacttgat
     4501 cttgaccgcc tcgtcggtgc cccgcagtat tgcgtcgagg accttcttgg gcaggtcctg
     4561 gaagggcgtg tcgaggctga acttgaagtg cttcgcgagg gcctcgaagc ggctgcggtt
     4621 ccaggccgcg tcggggttgt aggggatgca gccgccctcg ttgaaggaga gcgaggggtc
     4681 ggggatgacg aggtcggggt cgaactcgag cttgacccca aggcccgaac aggtcggaca
     4741 ggcgccctgg ggcgcgttga agctgaacag ccgcggctcc atctcgggca gcgagatccc
     4801 gcactccggg caggcgccgt tctggctgaa gaactcctcg cggggcggat cctcgtcgac
     4861 gaggaggatg acggtcccgt cggcgatctg gagggcggtc tcgaccgact cggccagccg
     4921 gcggcgcaag tcgtcggaga gcctgagccg gtccacgacg atctcgatgc tgtgcttcct
     4981 ctgcttctcg agcttgaagt ctatgggccc gcccgagggc agctccctga tctccccgtc
     5041 caccctggcg cggacgaagc cctgcttggt cgcgtcctcg aggatcttct tgtgctcgcc
     5101 cttctgcgcc ctgatcaccg gagccaggac ctggagcctg ctgccgctcg gccaggagag
     5161 gatcgtgtcg atgatctggt ctatgccctg ctcgcggatc tcgcggccgc actgggggca
     5221 gtggggcacg cccacccgcg cgaagaggag gcggtagtag tcgtagatct cggtgaccgt
     5281 ccccacggtg gagcgggggt tgcggtgggt ggtcttctgc tcgatcgaga tggccggcga
     5341 gaggccctcg atgtagtcga cgtccggctt gtccatccgg ccgaggaact ggcgggcata
     5401 ggccgacagg gactcgacat agcgcctctg gccctcggcg aagagggtgt cgaaggcggg
     5461 ggagctcttg cccgagcccg agaggcccga gatgacgatg agcttgtcgc ggggcagctc
     5521 gaggtcgatg ttcttgagat tatgctcgcg cgcgcctttg attatcagct tatccatgtt
     5581 gactacagag gatagccaag tctgccgccc caaggcaagg cccgacgctt aatttttgtg
     5641 tagtcattcg gcccctcccc gaccgggcca gcttcctgcc cgaaaccggg caggaaggcg
     5701 tcccgcggcc aagacctggc cccgggaggc ccccggggat cctcagcgtc gccttcggcc
     5761 ccccgggcag gcgggggcgc caagggcc
//
LOCUS       DASSQV010000486         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1356931, whole genome shotgun sequence.
ACCESSION   DASSQV010000486 DASSQV010000000
VERSION     DASSQV010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356931"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VFL04_08580"
     CDS             <1..1181
                     /locus_tag="VFL04_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7839803.1"
                     /translation="AAAIDENRLECEFTVVDEDGASVYSASDVAREEFPELDLTVRGA
                     ISIGRRLQDPLAELVKIDPKSIGVGLYQHDVNQKRLSESLDEVVGSVVNNVGVNLNTA
                     SHSLLRYVSGINNSLAKKIVKFREEKGGIPSRDRLREIPGLGDKTFEQCAGFLKIPES
                     PNLLDNTWVHPENYGLAHEILELVRGGGEPKGELRDALKARYEVSDTTINDIVAELKK
                     PNRDPRDNFPKPILQKGVVSFEDLAEGMKVTGKVKNVVDFGAFVDVGIKESALIHVSE
                     MSDRFVKDPMEVLKVGDVREFRIISLDPVRKRIGLSLKSERSEARAPAQAQSQGPRRD
                     DHRPAGPRPEGSERRGDARRVVAVRPSQTQGRGGPAPQKEDDGLTYNPFAELLKNRKK
                     "
     gene            1377..>2063
                     /locus_tag="VFL04_08585"
     CDS             1377..>2063
                     /locus_tag="VFL04_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839804.1"
                     /translation="MNTRVSAATKLQGSLGIICSTLYAGLLSHETMDVVDAGLADLSE
                     LFRHPLLPVYLGFAVASLASLRQGIMRAALPIGFLALTPIVSWESGNAFFGLGFSVVA
                     TILLARSGFFFRSPGLRAALLGAALTAAQLATAIGSRRPLATAAVCLASGGVFCLFLR
                     GVIQGGILASLTFMKEVVFLRDRGLSFREHDFVLARVAGKHPKVIAAENHVAVSTVRS
                     TLSTATRKLGL"
BASE COUNT          382 a          693 c          653 g          335 t
ORIGIN      
        1 tggccgcggc gatcgatgag aaccggctcg agtgcgagtt cacggtcgtg gacgaagacg
       61 gggcctcggt ctactcggcc agcgacgtgg cccgcgagga gttccccgaa ctcgacctga
      121 cagtgagggg cgcgatctcc atcggccgcc gcctccagga ccccctggcc gagctcgtga
      181 agatcgatcc caagtccatc ggggtgggcc tctaccagca cgacgtgaac cagaagcggc
      241 tctccgaaag cctcgacgag gtcgtgggct cggtggtcaa caacgtcggc gtgaacctga
      301 acacggcgag ccactccctc ctgcgctatg tctcgggcat caacaacagc ctggccaaga
      361 agatcgtgaa gttccgcgag gagaagggcg gcatccccag ccgcgaccgc ctgcgcgaga
      421 tcccgggcct cggcgacaag accttcgagc agtgcgccgg cttcctcaag atcccggaga
      481 gcccaaacct cctggacaac acctgggtcc accccgagaa ctacggcctg gcccatgaga
      541 tcctcgagct ggtgcgggga ggaggcgagc ccaaagggga gctccgcgat gccctcaagg
      601 ccaggtacga ggtgagcgac accacgatca acgacatcgt ggccgagctc aagaagccca
      661 accgcgatcc acgcgacaac ttccccaagc ccatcctcca gaagggcgtc gtcagcttcg
      721 aggacctcgc cgagggcatg aaggtcaccg gcaaggtcaa gaacgtcgtc gacttcggcg
      781 ccttcgtcga cgtcgggatc aaggagagcg ccctgatcca cgtctcggag atgtccgacc
      841 gcttcgtgaa ggatcccatg gaggtcctca aggtaggcga cgtgagggag ttccgcatca
      901 tctccctgga cccggtgcgc aagcgcatcg ggctctcgct caagagcgag cggagcgagg
      961 ccagggcccc ggcccaggct cagagccaag gaccccgccg cgatgatcac aggcccgcgg
     1021 ggccaaggcc cgagggctcg gaaaggcggg gcgatgccag gcgagtcgtg gccgtaaggc
     1081 cctcccagac ccagggcagg ggaggcccgg ctccgcagaa ggaggacgac ggcctcacct
     1141 acaacccctt cgcggagctc ctgaaaaacc gaaagaaatg ataatattac tgttccggtc
     1201 cgcataggga atggagattc cagaacccct ctgcgggccg gggcatgccc aattactcca
     1261 atagggcagc atccccacag gcgtcggtgc gaattccttc ggcggaagac tcatttttac
     1321 ggttttcgat ccggggcggt attgacaatt acgatgggcg gcggcaatcc gagggcatga
     1381 acacgcgggt gtcggctgcc accaaactcc aaggaagcct gggcatcatc tgttcgaccc
     1441 tctacgcggg gcttctctcc catgagacca tggacgtcgt cgacgcgggc ctcgctgacc
     1501 tctccgagct tttcaggcat cccctcttgc ctgtctatct cggcttcgcc gtcgcctcgc
     1561 tcgccagcct ccgccagggc atcatgcgcg ccgccctgcc catcggcttc ctcgccctga
     1621 cgcccatcgt ctcgtgggag agcgggaacg ccttctttgg cctgggcttc agcgtggtgg
     1681 ccaccatcct cctggcgagg tcgggcttct tcttccgaag tcccggactc agagccgctc
     1741 tccttggggc ggccctgacg gcggcccagc tcgccacggc catcggctcg cggcgtcccc
     1801 tcgccaccgc cgcggtctgt cttgcgagcg ggggagtctt ctgcctcttc cttcggggag
     1861 tgatccaggg cgggatcctc gcctcgctca ccttcatgaa ggaggtggtg ttcctcaggg
     1921 acaggggcct gagcttcagg gagcacgatt tcgtcctggc ccgggtggcg ggaaagcatc
     1981 ccaaggtgat cgcggccgag aatcacgtgg ccgtatccac cgtgaggagc accctgagca
     2041 cggccacgcg caagctcggt ctc
//
LOCUS       DASSQV010000487         6028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1976940, whole genome shotgun sequence.
ACCESSION   DASSQV010000487 DASSQV010000000
VERSION     DASSQV010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976940"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VFL04_08590"
     CDS             complement(<1..1232)
                     /locus_tag="VFL04_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839805.1"
                     /translation="MCDTMYAGPGSCRGGRSWFGKNSDRRPDEPQAVIIQGRRGAGST
                     LSFAGSEYSQVDDGLAYVLSRPSWMAGGEMGVNEAGVAIGNEAVFSRLHARKDGVLGM
                     DMLRAALASAADAKTAVDLICAATEAMDQGGRGSYHGGPSYSNSYLIADPRSAYVVET
                     AGRRWAWKPASELAAISNAYSIEEDYKRLDAQTRKEISPVNERAACSDEADAGRKGSK
                     ESWRAHVEERWPWGILKGDYRRAFVLSSLQGMRGSIDRSGFLGLLGSHAHRNPARLSG
                     SRMKAVCLHGGGIVNAATTASMAVEYLGEDGRGGNAALIWFTGTPFPCLSVYKPILLR
                     EDGFVPLWNGYDHSPNPTRDYAHWERHCAWARAKGRFALSLDPGFVERREALQTRIGE
                     AAEEALRGRIEHARAEVDA"
     gene            complement(1319..2212)
                     /locus_tag="VFL04_08595"
     CDS             complement(1319..2212)
                     /locus_tag="VFL04_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7839806.1"
                     /translation="MAHAVLERLGGSTRVVRGPANVGLFEAEDGKAVLIDSGNDDDSG
                     RRLLRFCEAEGLKLSLIVNTHSHADHCGGNAFLQSHTACRIAATGPEAAIIETPVLEP
                     SYLWGGYPLLPLRNKFLMAKPSRVTDPLTPPCDVPGTALKALPLGGHSYAMAGVMTPD
                     RVFFAADSIASIDVIKKYHVFFLYDIAAQLETLDALEVLDADWIVPSHAEPSRDMASL
                     IEVNRAKIVEIADFLVGSCAEPMTQEALLAGLALHYGIALNHQQFVLLGATVRSYLAW
                     LGQSRLVSSRLEAGKLLFERI"
     gene            complement(2193..3056)
                     /locus_tag="VFL04_08600"
     CDS             complement(2193..3056)
                     /locus_tag="VFL04_08600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7839807.1"
                     /translation="MKNRSALGSSFRIVALYALFAALWILFSDRLLLPFVHDPVTLMW
                     ISTGKGWLFVVVTASLLYLLISREMLRRSALENRLREGLANQEALTAEVHHRVKNNLQ
                     IITSILNLERDELVDEESRHLLDRTRARVYSMSLVHERLYEAQSFSRIDLGSYLRNLT
                     NALSDIYNHSSIRFDFELDSVEAEGAIAVPFGLLASEAITNSMKYGLGAGHVGRIRVS
                     LRDLGKGRAEFTVRDEGQGFPSAMAEGLGFRLMEALAAQLHGLLERNNEGGAVVRMNF
                     DLERTDGSRGS"
     gene            complement(3077..4105)
                     /locus_tag="VFL04_08605"
     CDS             complement(3077..4105)
                     /locus_tag="VFL04_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HET7839808.1"
                     /translation="MACYFFANIRVDDPSEYQKYLEGVEGLAARFGGRYLAVDTAPEV
                     LEGEWPYSRAVIIQFDNEDALRRWYASSEYRSIVGHRLAAGRSDSLLIHGEERDMDDR
                     IASGREFLKSNWNRLEEAEPDQAKGIPPPPQEDGAPEGLQPIALPPIGEISLAATTLR
                     DLIENRKSRRKYLEEALSLVELSYLLWAAQGIRERKSKYSFRTVPSGGARHPLDLYVY
                     ASHVSGLEEGLYRYLPLEHALCPVRLGSNSAGLDDALLGQLWNAAACFLWVAVPYRSE
                     WRYSVVAHKLVALDAGHSCQNLYLACESLGLGTCAVGAYDQVKTDAYLGLDGKDRFTI
                     YAAPIGRV"
     gene            complement(4116..4322)
                     /locus_tag="VFL04_08610"
     CDS             complement(4116..4322)
                     /locus_tag="VFL04_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839809.1"
                     /translation="MRPNQYSSEKRRKELEKKKKKEEKALRKAEQKNHPEGEAGAEGQ
                     IQTGEGDPSGAADGQGEAGKASDA"
     gene            complement(4446..5936)
                     /gene="glpK"
                     /locus_tag="VFL04_08615"
     CDS             complement(4446..5936)
                     /gene="glpK"
                     /locus_tag="VFL04_08615"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET7839810.1"
                     /translation="MAAKERYVLALDQGTTSSRAILFNKQGIVIGQKQIAFGQIFPKP
                     GWVEHDPEEIWTTQLAAARGAIESAQIEAGQIDSIGITNQRETTLIWDKATGKPLYNA
                     IVWQCRRSAEICAELRSLGLSALVRERTGLVLDPYFSGTKLMWLLKELPGLRARAERG
                     EILFGTVDSWLIYRLTGRHLTDPTNASRTLLFNINDGSWDAELLKVLKIPASMLPRVV
                     PSSGDFGIAGPEHFGVELPVSGCAGDQQAALFGHACFDPGSAKNTYGTGCFTLMNTGD
                     RPVASRHNLLTTIAWDLGKGRTYALEGSVFIAGAVVQWLRDQMGLIGDAAESERLARS
                     VEDSGEVFMVPAFVGLGAPHWDAEARGTVVGISRGTTKAHFVRAALESIAYQSRDLLV
                     AMEKDFGASIPYMKVDGGASANSFLMQFQADILGRRVILPEVGETTALGAAYLAGLAT
                     GFWESTSDVERNWRQRREFLPQMSQEARARLLGSWERAIGAARAYS"
BASE COUNT         1107 a         1917 c         1842 g         1162 t
ORIGIN      
        1 gcatcgacct cggccctcgc gtgttcgatg cggccacgaa gcgcctcctc tgccgcctca
       61 ccgatccttg tctgcaaagc ctcgcgccgc tccacaaaac cgggatccag ggagagggcg
      121 aaccggccct ttgcgcgtgc ccatgcgcag tgccgttccc agtgtgcgta gtcgcgtgtt
      181 gggttcgggc tgtggtcgta gccgttccaa agcggcacaa aaccgtcctc cctgagcaga
      241 atgggcttgt agacagagag acagggaaag ggcgtccccg tgaaccagat gagggcggca
      301 ttccctcccc ttccatcctc gcccaggtac tcgactgcca tactcgcggt cgtcgcggca
      361 ttgacgatgc cgccaccatg gaggcagacg gccttcatgc ggcttcccga caatcgggcg
      421 gggttcctgt gggcatggga cccaaggaga ccaaggaagc ccgacctgtc gatggagccc
      481 ctcatgcctt ggagcgagga aaggacgaag gcccgccggt aatcgccctt gaggatcccc
      541 catggccagc gctcttccac atgggccctc caggattctt tgctcccctt tcgaccggca
      601 tcggcttcgt ccgagcatgc ggctcgctca ttgacgggcg agatctcctt ccgcgtctgg
      661 gcgtcgaggc gcttgtagtc ctcctcgatg ctgtatgcgt tggagatggc agccaattcc
      721 gatgccggct tccatgccca gcggcggccg gctgtctcga ccacataggc cgacctggga
      781 tcagcaatca ggtagctgtt ggagtagctt gggccaccat ggtaggatcc gcgcccaccc
      841 tggtccatcg cctccgtcgc cgcgcagatg aggtccacgg cggtcttggc gtcggcggcg
      901 ctcgcgaggg cggcgcggag catgtccatg ccgaggaccc cgtccttcct tgcgtgcagc
      961 cgcgaaaaga cggcctcgtt cccgatcgcc acccccgcct cgttgacgcc catctccccg
     1021 ccggccatcc atgaaggcct cgacagaaca taggccagcc catcatcgac ctggctatac
     1081 tcggatccgg caaaagagag cgtcgagccg gcgccgcgcc taccctggat gatgaccgct
     1141 tggggctcat cgggcctccg gtcggagttc ttcccgaacc aggagcggcc tcctcggcag
     1201 gaacccggcc ctgcatacat ggtgtcgcac atggaaagcc cccttcgcgt atggtctcaa
     1261 ttcctcgtcg gtcagggaaa tccccgacag accgacccgg ctgtcagcgc atggactcct
     1321 agatccgctc gaacagaagc ttgccggcct cgagccttga ggagacaaga cggctttggc
     1381 cgagccatgc gagatatgag cgcaccgtcg cgcccagaag gacgaactgc tgatggttca
     1441 gcgcaatgcc atagtggagg gcgagcccgg ccaggagggc ctcttgagtc atgggctccg
     1501 cacagcttcc aaccagaaaa tcggcgatct cgacgatctt cgcccgattg acctcgatga
     1561 gcgatgccat gtcccggctg ggctcggcat ggctggggac gatccaatcg gcgtccagga
     1621 cctccagcgc atcaagggtt tccagctgcg cggctatgtc gtagaggaag aagacatggt
     1681 acttcttgat gacatcgatg ctggcgatcg agtccgcggc gaagaacacc ctgtctggtg
     1741 tcatgacccc cgccattgcg taggagtgtc caccgagggg gagggccttt agggccgtcc
     1801 cgggcacatc gcagggtggg gtcaggggat ccgtcacgcg ggaaggcttt gccatgagga
     1861 acttgttgcg gagcgggagg aggggatagc ctccccacag atagctcggc tcaaggactg
     1921 gggtctcgat gatggccgcc tcgggcccgg tcgcggcgat tcggcatgcc gtgtggctct
     1981 gaagaaaagc gttgccaccg caatggtcgg catgcgagtg ggtgttcacg atcagcgaga
     2041 gcttcaggcc ctcggcctca cagaatcgca ggaggcgccg acctgaatcg tcatcgttgc
     2101 ccgaatcgat gagaacagcc ttcccatcct ccgcctcgaa gagcccgaca ttggccgggc
     2161 cacgaaccac ccgggtgctg ccccccaagc gttcaagaac cgcgtgagcc atcggtccgc
     2221 tccaggtcga aattcatgcg aacgacggct ccaccctcgt tgttcctctc gagcaagcca
     2281 tgaagctggg ccgccagggc ttccatcagg cggaagccaa gcccctcggc catcgcgctg
     2341 ggaaagccct gcccctcatc gcgcacggtg aactcggccc gcccctttcc aagatcgcgc
     2401 aggcttacgc ggatccttcc aacgtgcccc gcaccgaggc cgtatttcat cgaattggtt
     2461 atggcctccg atgcgagcag gccaaaaggg actgcgatcg cgccttcggc ctcgacggaa
     2521 tccagctcga aatcgaatct gatgctggag tgattgtaga tgtccgagag ggcgttcgtg
     2581 agattgcgga ggtaggatcc cagatcgatc ctcgagaagg actgagcctc gtagagcctc
     2641 tcgtggacca gggacatgga gtataccctt gcccgagtcc tgtcgagcag gtgccgggat
     2701 tcctcgtcga caagctcgtc cctctccagg ttcagaatgc tggttatgat ctggagattg
     2761 ttcttgaccc gatggtgcac ttccgcagtg agggcttctt ggtttgccaa accctctctg
     2821 agtctgttct cgagggccga tcggcgcaac atctcacggg aaatcagcag gtaaagcagg
     2881 ctggccgtaa cgacaacgaa gagccagccc ttcccggtgg agatccacat gagggttacc
     2941 ggatcgtgaa cgaaaggcag gagtaagcgg tcggaaaaaa gtatccacag ggcggcgaac
     3001 agggcataca gggctacgat cctgaatgac gatcccagag ctgagcggtt tttcatctga
     3061 cctcgcgggc ggcaggctat accctgccga taggcgctgc gtagatggtg aagcgatcct
     3121 ttccatcgag gccaaggtag gcgtccgtct tcacttggtc gtaggccccc actgcgcagg
     3181 tcccaagacc aagcgattcg caggccagat agaggttctg gcaggaatgc ccggcgtcga
     3241 gggcaacgag cttatgtgcg acgacggagt agcgccactc gctccggtag gggaccgcga
     3301 cccagaggaa acaggctgct gcgttccaga gctgtcccaa gagggcgtcg tcaagcccgg
     3361 ccgaattgct gccgaggcgg accgggcaga gggcatgctc gaggggcaga tagcgataga
     3421 gcccctcctc caggcctgat acgtgggatg catagacata aaggtcgagg ggatgccggg
     3481 cgcctcccga ggggacggtg cggaaggaat acttcgattt tcgctctctg atgccctggg
     3541 cggcccagag gagataggac agttcgacga ggctcagggc ctcctcgagg tactttctcc
     3601 ggctcttgcg gttctcgatc aagtctcgaa gcgtcgtggc ggcgagactg atttctccaa
     3661 tcggcggaag ggcaatgggc tgcaaacctt cgggggctcc gtcctcctgc ggcgggggag
     3721 ggatgccttt tgcctggtcc ggctccgctt cctcgagccg attccaattc gacttcagga
     3781 actcacgacc tgaggcgatc ctgtcatcca tgtcccgttc ctccccatgg atcagcagac
     3841 tgtcggatct tcctgccgcc aacctatggc ctacgatcga tcggtattcc gaagaggcgt
     3901 accatcgcct gagcgcatcc tcgttgtcga attggatgat gacggccctc gaatacggcc
     3961 actcgccctc caacacctcg ggggccgtat ccaccgcgag gtagcgtccc ccaaaacgcg
     4021 ctgcaaggcc ttcgactccc tcgaggtact tctggtattc gcttggatca tcgaccctga
     4081 tgttggcgaa gaaatagcag gccatttagg cgatttcagg cgtcggaagc cttccctgcc
     4141 tcgccctgac catcggcagc accgcttgga tcgccttccc cggtctggat ctggccctcg
     4201 gcccccgctt ctccctcggg atggttcttc tgttcagcct tcctgagtgc cttctcttcc
     4261 ttcttcttct tcttctcgag ctctttcctg cgcttctcgc tgctatactg atttggccgc
     4321 acttttgctc cttaggacgc agaatcgggc gcgtcgcaag taattctagc acattggacc
     4381 ccgcgatgct accttgacgg ttctcccgag ctcccgatct gagcgggcca caggtcatcc
     4441 aggactcagg aatatgcccg cgccgcccct atcgccctct cccaagatcc gaggagccgg
     4501 gcgcgggctt cttgggacat ctgtggcagg aattcccgcc tctgcctcca attgcgctcg
     4561 acatcgctcg tcgattccca gaaacccgtg gcgagcccgg ccaaatatgc cgccccgagg
     4621 gccgtggtct ccccgacctc gggtaggatg acccgtcggc ccaggatgtc ggcctggaac
     4681 tgcatgagga agctgttggc tgaagcccct ccatccacct tcatgtaggg gatggatgct
     4741 ccgaaatcct tttccatggc gaccagaagg tcgcgggact ggtacgcgat ggattcaagg
     4801 gctgcccgca caaagtgcgc cttggtggtc ccccggctga ttcctacgac cgttccccga
     4861 gcctcggcgt cccaatgcgg agccccgagc ccgacaaagg ccggcaccat gaaaacctcg
     4921 cccgaatcct ccacggaacg ggcaagcctc tcgctctcgg cggcgtcgcc gatgagtccc
     4981 atctggtccc taagccactg cacgacggcc ccggcaatga agaccgagcc ctcgagggcg
     5041 tatgtccggc ccttccccag atcccaggcg atcgtggtca ggaggttgtg ccgagaggcc
     5101 acgggcctgt cgcccgtgtt catcagggtg aaacagcccg tgccataggt gttcttggcg
     5161 cttccgggat cgaaacacgc gtgtccgaag agggcagcct gctggtcccc cgcgcagccg
     5221 gagaccggaa gctcgacccc aaaatgctcg gggcccgcta tcccgaaatc ccccgaactc
     5281 ggaacgaccc ggggcagcat cgacgcgggg atcttgagga ccttgaggag ctcggcgtcc
     5341 caggacccgt cattgatatt gaagagaagc gtcctcgagg cgttcgtggg gtcggtcagg
     5401 tggcgacccg tgagacggta gatgagccag gagtcgacgg tgccaaagag tatttctccc
     5461 cgctcggcgc gggctcgaag cccgggcagc tctttgagca gccacatgag cttggtgccg
     5521 gagaagtacg gatcgaggac taggccggtc ctttcccgca ccagggcgga aaggcccaag
     5581 gaacgcagct cagcgcagat ctcggccgag cggcggcatt gccaaacgat ggcgttgtag
     5641 aggggttttc cggtcgcctt gtcccagatc agggtcgtct cccgctggtt cgtgattccg
     5701 atcgagtcga tctggccggc ttcgatttgg gctgattcta tggcaccgcg ggccgccgca
     5761 agctgcgtag tccagatctc ctcggggtcg tgctcgaccc atccgggctt ggggaagatc
     5821 tgtccaaagg ctatctgctt ttggccgatg acgattccct gcttgttgaa gagaattgcc
     5881 ctcgatgatg tcgttccctg gtccagcgcc agcacatacc gttccttggc cgccattgat
     5941 cccccctgtc gcgatcgatc gccctgtctc cgcgtgcggg acattatatg ggctatcgcg
     6001 tcgaatgtaa aaagcgccgg cgggtcac
//
LOCUS       DASSQV010000488         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_487490, whole genome shotgun sequence.
ACCESSION   DASSQV010000488 DASSQV010000000
VERSION     DASSQV010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487490"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2334)
                     /locus_tag="VFL04_08620"
     CDS             complement(<1..2334)
                     /locus_tag="VFL04_08620"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7839811.1"
                     /translation="MQQFSIVIKVIALVCYLILVLVVVQSRAEKNVRVFFAVYLFGML
                     FWQFTSLMASFSNTASSALFWYNLIIAGSGTFNILFFPFTRAFLKIKGQRLLTILGFV
                     ACAIMFATGILGLTWRQAVMGQGGYWVPVYNSGWLLVVGPIWYMFWLCGIFNLIRGLV
                     REESSVQRNRILYVLVGAVLVLAAVSTNLTALRDYPADTVLNLMSALIIGYAVLRYRL
                     LDIRFILARSIFYSVLTASLIAAYLGIVFGLEGALQESLGYNGPGYGIAAILVLSILF
                     LPLRNALQRILDRIFFRDRADYQSAIQAFSASMTSLYELDAILHLVGTTVGQTIKTSH
                     VSIALADEARKAFVAARRYGDGQMPSGELSINEQSRLVRWLRKEAKPLVREEALLDPA
                     SQPIVEESRSLFEEGGVSLVVPVLLNERLMGTLNLGEKRSGTIYTDEDLRFLTTIANQ
                     TATAVEKSSIFREIRRRLSEQTLLFILSEKFRSSMDFDSLMASIVQVLKSFLTCDQCA
                     IVSFDKTGEAKSYFLDADSQAAADLALLLRSELLTKPGAPTEDAPVPAELIRAKIRQR
                     PGLTEAQACTLAGLVYLPLKEGGELIGMLIVSNRTGAVDERELELLRTIRSIIMQGVM
                     LYRTIRNLMNLESYNENILNSLNDMGDTLLILDLNGSIKSVNRATCRTLGYSEEELVG
                     RDIGMITGDADSRFKTEVVRRLIAEGSISNYEVSYRASSGRLVPMLLSGSLMIGEDGK
                     TREIVGIARDMTEHHKVEEANKNLLLMQEIHHRIKNNL"
     gene            complement(2343..>2821)
                     /locus_tag="VFL04_08625"
     CDS             complement(2343..>2821)
                     /locus_tag="VFL04_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HET7839812.1"
                     /translation="TALEDAKAGVKLPQMTFVSRIDGLIDVGGFTRLTEKTIWQAIEN
                     AQVPYEEWTIRKEPSGGKPILHLYMELRNGEVGVDHIADRVHDSLKALDGPYRDLEEI
                     TGLKPLHVSLLSKGTFRRYYEERQAAGADLAHLKPPHVNASDAVIEGLQHMSVWRI"
BASE COUNT          486 a          805 c         1023 g          507 t
ORIGIN      
        1 gaggttgttc ttgatccggt ggtggatctc ctgcatgagg aggaggttct tgttggcctc
       61 ctcgactttg tggtgctcgg tcatgtcgcg ggctatcccg acgatctcgc gggtcttgcc
      121 gtcctccccg atcatgaggg agcccgacag gagcatcggg acgagccggc ccgagctcgc
      181 ccggtagctg acctcgtagt tggagatcga gccctcggcg atgagcctcc tgaccacctc
      241 ggtcttgaag cgggagtcgg cgtcgccggt gatcatgccg atgtcccggc ccacgagctc
      301 ctcctcggag tagccgaggg tcctgcaggt cgcccggttg acgctcttta tcgatccgtt
      361 caggtccagg atgaggaggg tgtcgcccat gtcgttgagg ctgttcagga tgttctcgtt
      421 gtaggactcg aggttcatca ggttgcggat ggtcctgtac agcatgacgc cctgcatgat
      481 gatcgagcgg atcgtgcgca ggagctcgag ctcgcgctcg tcgaccgctc cggtcctgtt
      541 ggagactatg agcatgccta tcagctcccc gccctccttc aggggaaggt acaccaggcc
      601 ggccagggtg caggcctggg cctcggtgag gcccggcctc tgcctgatct tggcgcggat
      661 cagctcggcg ggcacggggg cgtcctcggt gggggctcca ggcttggtca ggagctcgga
      721 gcgaaggagg agggccaggt cggcggcggc ctggctgtcg gcgtcgagaa agtaggactt
      781 ggcctcgccc gtcttgtcga agctcacgat ggcgcattgg tcgcaggtga ggaagctctt
      841 gaggacctgg acgatcgagg ccatcagcga gtcgaagtcc atggagctgc ggaacttctc
      901 gctcaggatg aagaggaggg tctgctccga gagccggcgc ctgatctcgc ggaagatgct
      961 gctcttctcc acggccgtgg ccgtctggtt ggcgatcgtg gtcaggaaac gcaggtcctc
     1021 gtcggtgtag atcgtccccg agcgcttctc gccgaggttc agggtgccca tgaggcgctc
     1081 gttgaggagg acgggcacga cgagcgacac tcccccctcc tcgaagaggg agcgcgactc
     1141 ctcgacgatg ggctgggagg cggggtcgag cagggcctcc tcgcgcacga ggggcttggc
     1201 ctccttgcgc agccagcgga cgagccggga ctgctcgttt atgctgagct cgcccgaggg
     1261 catctggcca tcgccgtagc gccgcgccgc cacaaaggcc ttcctggcct cgtcggcgag
     1321 ggcgatggag acgtgggagg tcttgatcgt ctgccccacc gtggtcccca cgaggtggag
     1381 gatcgcgtcg agctcgtaga gcgaggtcat ggaggcgctg aaggcctgga tcgcgctctg
     1441 gtagtcggcc cggtcgcgga agaagatcct gtcgaggatc cgctggaggg cgttgcggag
     1501 ggggaggaag agtatggaca gaacgaggat ggccgctatc ccgtagccgg ggccgttgta
     1561 gcccaggctc tcctggaggg cgccctcgag gccgaagacg atgcccaggt aggccgcgat
     1621 gagcgatgcc gtcagcaccg aatagaagat gctccgcgcg aggatgaagc ggatgtccag
     1681 gaggcggtag cgcaggacgg cgtagccgat gatcagggcg ctcatgaggt tgaggacggt
     1741 gtcggcagga tagtcgcgga gggcggtcag gttagtgctg acggcggcga ggaccaggac
     1801 ggcgcccacg aggacgtaga ggatgcggtt cctctggacc gaggactcct cccgcacgag
     1861 gccccggatg aggttgaaga tcccgcagag ccagaacatg taccagatcg gtccgacgac
     1921 gaggagccag cccgagttgt agaccgggac ccagtagccg ccctggccca tgacggcctg
     1981 cctccaggtc agccccagga tgcccgtcgc gaacatgatg gcgcaggcga cgaagccgag
     2041 gatggtcagg aggcgctggc ctttgatctt gaggaaggcc ctcgtgaagg ggaagaaaag
     2101 gatgttgaag gtgcccgatc cggcgatgat gaggttgtac cagaaaaggg ccgaggaggc
     2161 cgtgttcgag aagctcgcca tgagggaggt gaactgccag aacagcatgc cgaagaggta
     2221 gaccgcgaag aagaccctga cgttcttctc cgcccggctc tgcaccacga cgaggacgag
     2281 gatgaggtag cataccaggg cgatgacttt tatgacgatg gagaactgct gcatcgctgg
     2341 tatcagatcc tccagacact catatgttgc aggccctcga tgaccgcgtc cgaggcgttg
     2401 acgtggggcg gcttcaggtg ggccagatcg gccccggcgg cctgacgctc ctcataatac
     2461 ctccgaaagg tgcccttgga cagcaaggag acgtggaggg gcttgagccc cgtgatctcc
     2521 tcgaggtctc ggtaggggcc gtcaagggcc ttgaggctgt cgtggacgcg gtcggcgatg
     2581 tggtcgaccc cgacctcgcc gttgcggagc tccatgtaga ggtggaggat cggcttgccc
     2641 cccgatggct ccttgcggat ggtccactcc tcgtagggga cctgggcgtt ctcgatggcc
     2701 tgccagatgg tcttctcggt caggcgggtg aagccgccga cgtcgatgag gccgtcgatg
     2761 cgcgagacga aggtcatctg gggcagcttg acccccgcct tggcgtcctc gagggccgtg
     2821 a
//
LOCUS       DASSQV010000489         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_113573, whole genome shotgun sequence.
ACCESSION   DASSQV010000489 DASSQV010000000
VERSION     DASSQV010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113573"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFL04_08630"
     CDS             <1..204
                     /locus_tag="VFL04_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl peptide synthase"
                     /protein_id="HET7839813.1"
                     /translation="LDLFLIPDRPAAIRKAFSLAAPGDLVLLLGKGHENSIIYKEGPR
                     PYDEIGEAEKALAELGHTGMEHR"
     gene            201..1298
                     /locus_tag="VFL04_08635"
     CDS             201..1298
                     /locus_tag="VFL04_08635"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HET7839814.1"
                     /translation="MKRVVILYGGKSGEHEVSLISAASIVRHLDRGRYEPILVGIAKD
                     GSWILQDAGVLSEALAGEGPLPLRAGKAVLALASKGLAVLEEGGLLPLECDLVFPVLH
                     GTFGEDGTVQGFLEVAGLPYVGAGVLGSALGMDKEKSKELWLRAGLPVVPYIAVRRSE
                     LGSAAAIEGLARRIEARFGWPCFVKPACSGSSVGSSKVDGPRGLGAALDEALAWDEKA
                     MVEPFVSAREIECAVLGNASPRAFPPGEVIPSHEFYDYEAKYLDPQGARFEIPARLSA
                     EQAERVTTVAVAAFKACECSGMARVDFFVDKRTGEVLLNELNTIPGFTHISMYPKMCE
                     SGGLAYAGLIERLLDLALERHAAREALRYSR"
     gene            1537..>2602
                     /gene="rocD"
                     /locus_tag="VFL04_08640"
     CDS             1537..>2602
                     /gene="rocD"
                     /locus_tag="VFL04_08640"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HET7839815.1"
                     /translation="MKSQDYIALDEKYGAHNYHPLPVVIAWAEGCKVKDPEGKTYFDF
                     LSAYSAVNQGHRHPRILKAVRDQLELCTLTSRAFHNDRMGLFLKKLCEFTGFETALPM
                     NTGAEAVETALKAARRWGVEVKGVKNGEQEIICAEENFHGRTIAVISMSTDPDSTVNY
                     GPYVPGCTKVPYGDAEAIEKSITPKTVAVIIEPIQGEAGVCVPPAGYLKRVREICTKH
                     KVLFIADEIQTGFSRTGKRFAWQHEDARPDIMCLGKALGGGIFPISAIVADHDVMKVF
                     TPGTHGSTFGGNPLASAIGIAAIDVLVDEKLDENAERLGRIFREEIGKIRCPKMKLVR
                     GKGLLNAVLFEPGFEAWNVCL"
BASE COUNT          465 a          919 c          799 g          419 t
ORIGIN      
        1 ctggacctct tcctcatacc cgacaggccc gcagcgatcc gcaaggcttt ttccctcgcc
       61 gcgcccgggg acctcgtcct cctcctcggc aagggccacg agaactcgat catctacaag
      121 gaagggccca ggccctacga cgagataggc gaggccgaga aggccctcgc agaattggga
      181 cacacaggaa tggagcatcg atgaaacgcg tggtgatcct ctacggcggc aaatcgggcg
      241 agcacgaggt ctcgctcatc tcggcggcga gcatcgtccg ccatctggac cgcgggcgct
      301 atgagcccat cctcgtcggc atcgcgaagg acggctcctg gatcctccag gacgccgggg
      361 tcctctccga ggccctcgcg ggagagggcc ccctgcccct ccgcgccggc aaggccgtgc
      421 tcgccctggc ctccaagggc ctcgcggtgc tcgaagaagg gggtctcctt cccctcgagt
      481 gcgacctggt ctttccggtg ctccacggga ccttcggcga ggacgggacg gtccagggct
      541 tcctcgaggt ggctggcctg ccctatgtcg gggccggggt cctcggctcg gccctcggca
      601 tggacaagga gaagtcgaag gagctctggc tccgcgcagg cctgcccgtc gtgccctaca
      661 tcgcggtgcg ccgctccgag cttggctcgg ccgcggcaat cgaaggcctc gcccgcagga
      721 tcgaggcccg tttcggctgg ccctgtttcg tgaaaccggc ctgctccggc tcctcggtcg
      781 gctcatcgaa ggtcgatggc ccccgaggcc tcggagcggc cctcgacgag gccctcgcat
      841 gggacgagaa ggccatggtc gagcccttcg tcagcgcgcg agagatcgag tgcgctgtcc
      901 tgggcaacgc cagccccagg gccttcccgc ctggcgaggt catcccgagc cacgagttct
      961 acgactatga ggcgaaatac ctcgaccccc agggcgcccg tttcgagata ccggcccgcc
     1021 tgagcgccga acaggccgag cgcgtcacca cggtcgccgt cgcggccttc aaggcctgcg
     1081 agtgctcggg catggcccgg gtcgacttct tcgtcgacaa aaggacgggc gaggtcctcc
     1141 tcaacgagct gaacaccatc cccggcttca cccacatctc gatgtacccg aagatgtgcg
     1201 agtcgggggg cctggcctat gccgggctca tagagaggct cctcgatctg gccctcgaac
     1261 gccacgcggc cagggaggcc ctcagatact cgcgataggg gctttcgcat aatcatgcag
     1321 ggctttgcaa tatttatcta ccatgtatga aattataaga acggggctgg cgcgaatgcc
     1381 gttgggatgc tatagtggcc acacagcttc ccccacatca gcgcatgatc gatgatccgc
     1441 ctcgcgagga tgtgagtacg ggcctgcggg cctgtgcgcc tgccgccgac ccgatcggct
     1501 ctggcttggc gaagggaaag caaggaggtt cacaccatga aatcccagga ctacatcgcc
     1561 ctggatgaga agtacggggc gcacaactac catccccttc ccgtagtcat cgcctgggcc
     1621 gaaggctgca aggtgaagga tccggaaggc aagacatact tcgacttcct ctccgcctat
     1681 tccgcggtca accagggcca ccgccacccg aggatcctca aggccgtccg cgaccagctc
     1741 gagctctgca ccctcacgag ccgggccttc cacaacgacc gcatgggact cttcctcaag
     1801 aagctctgcg agttcaccgg cttcgagacc gccctcccca tgaacacggg tgccgaggct
     1861 gtcgagacgg cgctcaaggc cgcccggcgc tggggcgtcg aggtcaaggg cgtgaagaac
     1921 ggcgagcagg agatcatctg cgccgaggag aacttccacg gccgcaccat cgccgtcatc
     1981 tccatgtcca ccgatcccga ctccacggtc aactacggcc cctatgtccc gggctgcacc
     2041 aaggtcccct acggcgacgc cgaggccatc gagaagtcca tcacccccaa gacggtcgcc
     2101 gtcatcatcg agccgatcca gggcgaggcc ggcgtctgcg tgccgcccgc cggctatctg
     2161 aagcgcgtgc gcgagatctg caccaagcac aaggtcctct tcatcgccga cgagatccag
     2221 accggcttca gccgcaccgg caagcgcttc gcctggcagc acgaggacgc ccgccccgac
     2281 atcatgtgcc tcggcaaggc cctcggcggc ggcatcttcc cgatctccgc catcgtcgcc
     2341 gaccatgacg tgatgaaggt cttcaccccc ggcacccacg gctccacctt cggcggcaac
     2401 cccctcgcca gcgccatcgg catcgccgcc atcgacgtcc tcgtcgacga gaagctcgac
     2461 gagaacgccg agcggctcgg caggatcttc agggaggaga tcggcaagat ccgctgcccc
     2521 aagatgaagc tcgtgcgcgg caagggcctc ctcaacgctg tgctcttcga gcccggcttc
     2581 gaggcctgga acgtctgtct gg
//
LOCUS       DASSQV010000490         4048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2228640, whole genome shotgun sequence.
ACCESSION   DASSQV010000490 DASSQV010000000
VERSION     DASSQV010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2228640"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFL04_08645"
     CDS             complement(<1..710)
                     /locus_tag="VFL04_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839816.1"
                     /translation="MSRLSPIPLISALLILLARPLSAQAVSGTNAAPAPPGQTGSVAQ
                     PARTWMLGLCRLSVGELPADFRLLAASLPQLIAAELQVLPPRLASPEEFLESSDLAAE
                     RARYEAGGEFAKQLDLRAAKFLDPSIPRARREAEISAADRNLALASKHYGEALEKAGQ
                     NPEPQPDSGLPAHLSGANAKGELIDPPAVSPAVSAKAKALDFLVFGRVSIESDYAAVE
                     LSGYDASLDRTVFTWKGF"
     gene            complement(740..1880)
                     /gene="prfB"
                     /locus_tag="VFL04_08650"
     CDS             complement(join(740..1810,1812..1880))
                     /gene="prfB"
                     /locus_tag="VFL04_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702248.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET7839817.1"
                     /translation="MLEDIAVPVSSLRAEILDIWGRLDSVGMERRIAEKEALSSDPGC
                     WNDGAKAGRVMTEIKVLRDRLDTWVQLKEDVSALDEVIQMSREEGDDSMESDIRNSYK
                     DISTRFDKAIVLELLSGEADRSGAYLSIHAGSGGTEACDWASMLLRMYTRWAERRKFK
                     ADIVDLLEAEGGVKSATLEIDGDFAYGYLKGESGVHRLVRISPFDANARRHTSFASVY
                     ILPILDDSIVVDIRPEDLRIDTYRAGGAGGQKVNKTDSAVRLTHLPTGIVVTCQNERS
                     QYKNKDVAMSVLKSRLYDHYTLEKEKENAKFAAEKKDIAWGSQIRSYVFQPYTLVKDH
                     RNKFSVGNVQSVMDGEIDPFIESFMRWRWQGGNATATAADEDEEL"
     gene            complement(1959..2255)
                     /gene="cas2"
                     /locus_tag="VFL04_08655"
     CDS             complement(1959..2255)
                     /gene="cas2"
                     /locus_tag="VFL04_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HET7839818.1"
                     /translation="MDMFVSVICDMGSEDSRSALYNLLPQYGFERVQKACFECSTMNE
                     KLLGALKRDIDRITDSYDTIRFYQFPVEGTLALTVLKENKWRRIVVRDPTRPRE"
     gene            2348..3673
                     /locus_tag="VFL04_08660"
     CDS             2348..3673
                     /locus_tag="VFL04_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839819.1"
                     /translation="MRSLEKIGTIAIAAFGLLVLPLPAQVRDALPNAGPAGQETSSEA
                     PEAVFAPFPSHLRAATKAGTIVLTWTDSSDVKGRYAVYRSSRAITVETFGTATRIGEV
                     GPGIERYEDSPPDDSAYYYLILALAEDGSPYRIFIPARNETLSPIAIEKTAVSPAAPP
                     APAPAPAPSLAVDGIDAQVHGDAIIVSYRPSNAGMRLVLYRGTAPMTTGRDLLEASLV
                     AAFDDKEGSFADYPVPGVDYWYAVLAEDELKAGRVELAAGHNSTSKATRIAAALSQGD
                     FIEPSPTRTPPLPSILLEKSAGTGSSALPRGGDAPQLGSLSPEAEKSVAALMRLAPPV
                     REVLPRVRILKEEQSSPTGGEDYALSLIVREKLGRQDWSGAADQLRKYLSLNRSPAAA
                     ARAHFYLGEALAESGSYREAFFELISARAGLMVETKPWIDYILAILRQG"
     gene            complement(3677..>4048)
                     /locus_tag="VFL04_08665"
     CDS             complement(3677..>4048)
                     /locus_tag="VFL04_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET7839820.1"
                     /translation="VKGTPFDFGGAKLIGRDIEAAGGYDHCFVVDRKGPGLVEFAEVF
                     EPMTRRRMSVATTLPGVQFYTGNFLSNVAGKRGSIYDKHAGFCLETELFPDSPNKPAF
                     PSAVVEPGSLWKHQTVYRFQP"
BASE COUNT          704 a         1327 c         1289 g          728 t
ORIGIN      
        1 caaaacccct tccaggtgaa gactgtccga tcgagggagg cgtcatagcc gctcagctcc
       61 accgcggcgt agtccgactc gatgctgacc ctcccgaaca cgaggaagtc cagggccttg
      121 gccttggcgc tgaccgccgg cgagacggca gggggatcga tgagctctcc ctttgcgttc
      181 gcccccgaga gatgggcagg tagcccggaa tcgggctggg gctcagggtt ttgccccgct
      241 ttctccaagg cctcgccata gtgcttgctc gccagggcca ggttcctgtc cgcggccgag
      301 atctccgcct cgcggcgcgc cctgggaatc gagggatcga ggaacttggc tgccctgagg
      361 tcgagctgct tcgcgaactc tcctccggct tcgtacctcg cccgctcggc ggcgagatcc
      421 gaggattcaa ggaactcctc cggagaggcg agccgaggag gaaggacctg gagctcggcc
      481 gcgatgagct ggggcagact cgccgcgagg agacggaaat cggcggggag ctcgccgacg
      541 gaaagccggc agaggccgag catccaggtc ctggcgggct gggccacgga tcccgtctgg
      601 ccgggcggcg cgggcgccgc attcgtgccg ctgaccgcct gggcggagag gggacgggca
      661 aggaggatca ggagggctga gatgagcgga atcggactga ggcgcgacat cggcatggca
      721 ccaaggccgc ggcccatcct cagagctcct cgtcctcatc ggccgcggtc gcggttgcgt
      781 ttccgccctg ccaccgccac cgcatgaagg actcgatgaa gggatcgatc tccccatcca
      841 tgaccgactg gacattgccc accgagaatt tgttgcggtg gtccttcacg agggtatagg
      901 gctggaagac gtaggacctg atctgcgagc cccaggcgat gtccttcttc tcggcggcga
      961 acttcgcgtt ctccttctcc ttctcgaggg tatagtggtc gtagagccgg gatttcagga
     1021 cgctcatcgc gacgtccttg ttcttgtact ggctgcgctc gttctggcag gtgacgacga
     1081 tgcctgtcgg cagatgggtc aggcggacgg ccgaatcggt cttgttgacc ttttgcccgc
     1141 ccgctcctcc ggcgcggtag gtgtcgatgc ggaggtcctc cggccttatg tcgaccacaa
     1201 tcgagtcgtc gaggatgggg aggatataga ccgaggcaaa agatgtgtgg cgtcgggcat
     1261 ttgcgtcgaa gggggagatc cggacgagcc tgtggacgcc cgactcgccc ttgagatagc
     1321 cataggcgaa gtccccgtcg atctcaaggg tggccgactt cacgcctccc tcggcctcaa
     1381 gcaggtcgac gatgtcggcc ttgaattttc tgcgctcggc ccacctcgtg tacatgcgga
     1441 ggagcatgga tgcccagtcg caggcctcgg tcccgcccga tccggcgtgg atcgagaggt
     1501 acgcccccga tctgtcggcc tcgccggaca ggagctcaag cacgatggcc ttgtcgaagc
     1561 gcgtggagat gtccttgtag gagttgcgga tgtccgattc catggaatcg tccccttcct
     1621 cacgggacat ctggatcacc tcatcaaggg cggacacgtc ctccttgagc tggacccagg
     1681 tgtccagacg atccctcagg accttgatct cggtcatgac ccgaccggcc ttggccccgt
     1741 cgttccagca gcccggatcg gagctcagcg cctccttctc ggcgatcctg cgctccatgc
     1801 cgaccgagtc aaagacgccc ccagatgtcc aggatctccg cacgcaggga gctgacgggg
     1861 acggcgatat cttcaagcat ctggttcctc cgaagcaggt ggtattggga tttgggggac
     1921 acgggggcgc ggacggatcc ggagcctggc ctgtcgggtc attccctggg gcgggtcgga
     1981 tccctcacca cgatcctgcg ccatttgttt tccttcagga cggtcagcgc cagggtgccc
     2041 tcgaccggga attggtagaa ccgtatcgtg tcataggagt cggttatgcg atcgatgtcc
     2101 cgcttcagcg cgcccaggag cttctcgttc atggtggagc actcgaaaca ggccttctgg
     2161 actcgctcga aaccgtactg tggcaggagg ttgtacagcg cggaccttga gtcttcgctc
     2221 cccatgtcgc atatgaccga aacgaacatg tccatatcgt acccgaagat gggggcaccg
     2281 tcaattcgag ggcccttccc aaggagagct atgcaatcgg acgctccgat ccgatactat
     2341 ttgtaccatg cgcagcctgg aaaaaatcgg cacgatagcc atcgcggcct tcggcctcct
     2401 cgtccttccc ctgcccgccc aggtccggga tgcccttccg aatgcgggtc ccgcaggaca
     2461 agagacctcc tcagaggctc cggaggcggt attcgccccc ttcccctcgc atctgcgggc
     2521 agcgaccaag gccggcacca tagtgctcac ctggaccgac tcaagcgacg tcaagggccg
     2581 ctatgccgtt tataggagca gccgcgccat caccgtcgag accttcggaa ccgctaccag
     2641 gatcggggag gtgggccccg gcatcgagcg ctatgaggac agcccccccg acgactcggc
     2701 ctactattat ctcatccttg cccttgccga ggacgggagc ccctaccgga tcttcatccc
     2761 cgccaggaac gagaccctga gccccatagc catcgagaag acggcggtga gccccgcggc
     2821 cccgcccgcc cctgcccccg ctccggcccc cagccttgcg gtggatggca tcgatgccca
     2881 ggtccacggt gacgcgatca tcgttagcta tcgcccctcg aacgccggca tgcgcctggt
     2941 cctctaccgc gggacggctc cgatgacgac cggaagggac ctcctcgaag cctccctggt
     3001 cgccgccttt gatgacaagg agggcagctt cgccgactat ccggtccccg gtgtcgacta
     3061 ctggtatgcg gtgctcgccg aggatgagct gaaggcgggg cgcgtcgagc tcgcggccgg
     3121 gcacaattcc acaagcaagg ccacaaggat agcggccgcg ctgtcgcagg gcgatttcat
     3181 cgagccttca cccacgagga cgcccccgct gccatcgatc ttgcttgaga aatcggcggg
     3241 aacgggctcg agcgccctgc cccgaggcgg ggacgctccc caactaggct ctctgagccc
     3301 cgaggccgag aaatcggttg cggccctcat gaggcttgcg cccccggtcc gcgaggtact
     3361 gcccagggtc agaatcctca aggaggagca gtcctcgccc acgggaggcg aggactatgc
     3421 cctctcgctc atcgtcaggg agaaactcgg gcgccaggac tggtctggag ccgcggacca
     3481 gctccgcaag tacctctcgc tcaaccgctc gcccgcggcg gccgcgaggg cccatttcta
     3541 cctcggcgag gccctggcgg agtcgggatc ctaccgcgaa gccttcttcg agctgatatc
     3601 ggccagggcc ggcctcatgg tggagaccaa gccctggatc gactacatcc tggccatact
     3661 ccgccaaggc tagggtctag ggctggaacc tgtagacggt ctgatgcttc caaagcgagc
     3721 caggctcgac gacggcgctt ggaaacgcgg gtttgttggg cgagtcgggg aatagctcgg
     3781 tctccaggca gaagccggcg tgcttgtcgt agatcgagcc cctcttgccg gcgacgttgg
     3841 agaggaagtt ccccgtatag aactgcaccc ccggcagcgt ggtcgcgacg ctcatcctgc
     3901 gtcgcgtcat gggctcgaag acctcggcga actccacaag gccggggccc ttcctgtcca
     3961 ccacaaagca gtggtcatag ccgcccgccg cctcgatgtc cctcccgatg agcttggctc
     4021 cgccaaaatc gaagggcgtg cccttcac
//
LOCUS       DASSQV010000491         3723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_114435, whole genome shotgun sequence.
ACCESSION   DASSQV010000491 DASSQV010000000
VERSION     DASSQV010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114435"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFL04_08670"
     CDS             complement(<1..494)
                     /locus_tag="VFL04_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7839821.1"
                     /translation="MSSQINIGVIGAGRIGKIHAENIAYFMPEARLAAIADLNLTPAI
                     EAWAKKLGVATVTKDPKALLADKSIQAVLICSSTDTHADFVIEAAKAGKHVFCEKPVD
                     LTVDKVRRALAAVEKAGVKLQVGFNRRFDHNFRKVRELVQSGALGEPHLIRITSRDPT
                     PPPA"
     gene            complement(571..1407)
                     /locus_tag="VFL04_08675"
     CDS             complement(571..1407)
                     /locus_tag="VFL04_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET7839822.1"
                     /translation="MIDRNRFALNRITSPSLSLAAFYDLAVSVGLKKVEIRNDLGKAD
                     PVDGLGAAAAAKLAAERGLEVVTINALQKFNLPSARAKATAELKALLELASGIGCKAI
                     VLCPNNDGSDKRSAPERLADTAQALADYAPLFTKAGVAGYLEPLGFGISSLASLAVAA
                     EAIARSKAGCYKVVVDTFHFHIGPDSPEVFGTGFPIASAGLVHISGVELDMPKADYRD
                     EHRVLVGPADRMKSKEQILRLEKLGYRGVYSFEPFSPLVQDLKGPALVSALKASLDYL
                     VS"
     gene            complement(1461..2477)
                     /locus_tag="VFL04_08680"
     CDS             complement(1461..2477)
                     /locus_tag="VFL04_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7839823.1"
                     /translation="MSVKKLRIGIVGLGRLGKRHALNLAGLPEVALAAACSLGQGELE
                     WARAELGVEALFSSYEAMIASGKIEAAFIVSSSSAHASQAVQALRQGLHVFCEKPIGV
                     TVEECLEVERAAEAAPGLVFLPGFVRRFDPSYAYAKGLVDQGAIGTPFLVRSQTVDLD
                     EYAAFQVKFVPSSGGIFLDMNIHDIDLARWFLADEVESVHAMGGSFVHPEFAAVGDAD
                     NTVALARFRTGRMAVLSVSRTAFHGHDTHTEITGSKGILKIGMTPARNRVELYDGAGA
                     RVECVRDFYERFAEGFQAEAREFVGCALEGRRPSVTARDGRAATEVGFALTESFRSGS
                     IVRI"
     gene            2675..3700
                     /locus_tag="VFL04_08685"
     CDS             2675..3700
                     /locus_tag="VFL04_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7839824.1"
                     /translation="MTSIKDLARLAGVSASTISRAVNNKKYVKPEIRERIMELVKETG
                     YVPNNAARAMVLKRTFTVGIVIPDTFNMFQRQLFSTIERSLEGFGFHTSFFFVVWEPE
                     SELRCLRRLKAEKLDGVIMVHEAARPEFYDYLAKSQVPTVLCTFSRGGFHFPSVHVDE
                     EAAARTATEHLIGLGHRRIGLISGTHFSFGAQRASGYRAALAGAGIGLDEADTVLMAS
                     YTPEEGRSGMRCLLERGRGLTAVFASTDELAIGAVRALYEAGLSVPRDVSVIGLDDID
                     ISAYLSPGLTTVRQPIREIGRRTAEIMARLIEGEAGVEGEAGAGDSVFGHELILREST
                     ARPAGQS"
BASE COUNT          570 a         1246 c         1290 g          617 t
ORIGIN      
        1 tcggccgggg gcggggtggg gtcgcgcgag gtgatgcgga tcaggtgcgg ctcgcccagc
       61 gccccggact gcaccagctc gcgcaccttg cggaagttgt gatcgaagcg gcggttgaag
      121 cccacctgga gcttcacgcc cgccttctcc accgcggcca gcgcccgccg caccttgtcc
      181 accgtcaggt cgaccggctt ctcgcagaag acgtgcttgc cggccttggc ggcctcgatc
      241 acgaagtcgg catgggtgtc ggtggaggag cagatgagca cggcctgaat ggacttgtcg
      301 gcgaggaggg ccttggggtc cttggtcacc gtcgccacgc cgagcttctt ggcccaggcc
      361 tcgatggcgg gagtcaggtt caggtcggct atcgctgcga gcctggcctc gggcatgaag
      421 taggcgatgt tctcggcgtg gatcttgccg atcctgcccg ctccgatgac gccgatgttg
      481 atctgggacg acattgctgg acctcctgtg gaactcaagg cccgcccttt ccggcgggcc
      541 gcacggtttt cggaaaacga ttcggggcga tcaggacacg agatagtcga ggctggcctt
      601 cagggccgag acgagggcgg ggcccttgag gtcctgcacg aggggcgaga agggctcgaa
      661 ggaatagacc ccgcggtagc cgagcttctc gagcctcagg atctgctcct tgctcttcat
      721 cctgtcggcg ggaccgacga ggacgcggtg ctcgtcgcgg tagtcggcct tgggcatgtc
      781 aagctcgacc ccggatatgt gcacgaggcc agcggaggcg atggggaagc cggtcccgaa
      841 gacctcgggg ctgtcgggtc cgatgtggaa atggaaggtg tcgacgacca ccttgtagca
      901 gcccgccttg gacctggcta tcgcctcggc ggcgacggcg agggaggcga gcgaggagat
      961 gccgaagccg aggggctcga ggtagcccgc taccccggcc ttggtgaaca ggggggcgta
     1021 gtcggcaagg gcctgggccg tgtcggcgag gcgctcgggg gcgctccttt tgtccgaacc
     1081 gtcgttgttc gggcagagga cgatggcctt gcagccgatc ccggaggcga gctcgaggag
     1141 ggccttcagc tcggccgtcg ccttggcccg ggccgagggg aggttgaact tttggagggc
     1201 gttgatggtg accacctcga ggccccgctc ggcggcgagc ttggcagcgg cggcggcccc
     1261 caggccgtcc accgggtcgg ccttcccgag gtcgttcctg atctccacct tcttgaggcc
     1321 caccgagacc gccaggtcat agaaggcggc gaggctcagg ctgggtgagg tgatcctgtt
     1381 cagcgcaaaa cggttcctgt ctatcatggc gatgctcctc ctggccaaag gtcccgaccg
     1441 gccccttggg gccgcggccc tcagatcctg acgatgctcc cgctccggaa ggactcggtg
     1501 agggcgaagc ccacctcggt cgccgccctt ccgtcccgcg ccgtgacact cggcctcctc
     1561 ccctcgagcg cgcagccgac gaactcgcgg gcctcggcct ggaagccctc ggcgaaacgc
     1621 tcgtagaaat ccctgacgca ctcgacccgg gccccggcgc cgtcgtagag ctcgacccgg
     1681 ttcctcgcgg gggtcatccc gatcttgagg atgcccttgc tgcccgtgat ctcggtgtgg
     1741 gtgtcgtggc cgtggaaggc cgtcctcgag acgctcagca cggccatcct gcctgtcctg
     1801 aacctggcca gggccacggt gttgtcggcg tcgccgacag ccgcgaactc gggatggacg
     1861 aagctcccgc ccatcgcgtg gacgctctcc acctcgtcgg cgaggaacca cctcgcgagg
     1921 tcgatgtcgt ggatgttcat gtcgaggaag atcccgcccg agctggggac gaacttgacc
     1981 tggaaggcgg cgtactcgtc gaggtcgacc gtctgggagc gcacgaggaa gggggtcccg
     2041 atggcgccct ggtcgacgag gcccttggcg taggcgtagc tcgggtcgaa gcgccggacg
     2101 aagcccggca gaaagacgag gcccggcgcc gcctcggcgg cccgctcgac ctcgaggcat
     2161 tcctccaccg tgaccccgat gggcttctcg cagaagacgt ggaggccctg tctgagggcc
     2221 tggacggcct ggctcgcgtg ggccgaggag gagctcacga tgaaggccgc ctcgatcttc
     2281 ccgctggcga tcatggcctc gtagctggag aacagggcct cgacgccgag ctcggcgcgg
     2341 gcccactcga gctccccctg cccgaggctg caggcggcgg ccagcgcgac ctcggggagc
     2401 cccgcgaggt tgagggcatg ccgcttgccg aggcgcccga ggccgacgat cccgatccgg
     2461 agctttttga ctgacattcc cgagcctcca ccggtggcgg caatcgcttg cagttttgtt
     2521 gcaaacgttt tcagacaatg ccgattatca atcgggatcg ggctcctgtc aatcgatttc
     2581 ggcgcgtctt cgggcccggc cggcgtcctt cccgtccctg atcccttgtg ccgaccaagg
     2641 cgctggggat agattcagat tccgaggggc cagcatgacg agcatcaagg accttgccag
     2701 actcgcgggg gtgtcggcat cgaccatctc ccgggccgtc aacaacaaga aatacgtgaa
     2761 accggagatc cgagagcgca tcatggagct cgtcaaggag accggctacg tccccaacaa
     2821 cgcggcgcgc gcgatggtcc tcaagcgcac cttcaccgtc ggcatcgtga tccccgacac
     2881 cttcaacatg ttccagcgcc agctcttctc caccatcgag cgcagcctcg agggcttcgg
     2941 cttccacacc tccttcttct tcgtggtctg ggagcccgag agcgagctgc gctgcctgcg
     3001 gcgcctcaag gccgagaagc tcgacggcgt catcatggtc catgaggcgg ccaggcccga
     3061 gttctacgac tacctcgcga agtcccaggt cccgacggtg ctctgcacct tctcgagggg
     3121 tggcttccac ttcccctcgg tccacgtcga cgaggaggcc gccgcgagga cggcgaccga
     3181 gcacctcatc ggccttggcc accgcaggat cggcctcatc tcggggaccc atttctcctt
     3241 cggcgcccag cgcgcctcgg gctacagggc tgccctcgcc ggagccggca tcgggctcga
     3301 cgaggccgac accgtcctca tggcctccta cacccccgag gaggggcggt cgggcatgcg
     3361 ttgcctcctc gagcgcgggc ggggcctcac ggccgtcttc gcctcgaccg acgagctggc
     3421 catcggcgcg gtgcgcgccc tctacgaggc cgggctctcg gtcccgcgcg acgtctcggt
     3481 catcggcctc gacgacatcg acatcagcgc ctacctctcg cccggcctca cgacggtgag
     3541 gcagccgatc agggagatcg gcaggaggac ggccgagatc atggcccgcc tcatcgaggg
     3601 cgaggccggg gtcgagggcg aggccggggc cggcgactcg gtcttcggcc acgagctcat
     3661 cctgagggag tcgacagccc ggccggcggg gcagagctag gggcggcccc ctagaggggc
     3721 cag
//
LOCUS       DASSQV010000492         4903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2229353, whole genome shotgun sequence.
ACCESSION   DASSQV010000492 DASSQV010000000
VERSION     DASSQV010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229353"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFL04_08690"
     CDS             <1..317
                     /locus_tag="VFL04_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839825.1"
                     /translation="AELEPRIAALPNVRTLRRDAFSLLPKEVGRVDWLCSDVICYPPA
                     LLKWVELWLESGLARRFICTIKMQGEGFDRETADRFAAIPGSRVVHLWHNRHELTWIK
                     TE"
     gene            complement(325..1362)
                     /locus_tag="VFL04_08695"
     CDS             complement(325..1362)
                     /locus_tag="VFL04_08695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7839826.1"
                     /translation="MPVLREYKKKENAIYRRFSARLGRAAHRTGRGLARLFSGGRQKL
                     TIMVIPHDERKIRDVQLSFFGVAGIALASVALIVAFGLSLAHISGSRRQLDQDSQDLA
                     AARANLDSLREQTGRLATAAKQFETALSNSLASVGVAVPASESPGPGGSLSSFLGIDS
                     SGGRDADELSKLDRLLEQSSAPIEQIGYLLRNQNAVMSDIPNIWPIKGGIGHISMYFG
                     QNTNPFTGQWYTHTGLDISTFGQGDPIVATADGTVVSLSYESGGYGNNIILRHKHGFY
                     TRYAHLQAFRVRRGQEVKQGQVIGILGNTGLTTGPHVHYEVRLGRDLIDPTKFLNVGA
                     SAIVLSDKPAD"
     gene            complement(1487..3652)
                     /locus_tag="VFL04_08700"
     CDS             complement(1487..3652)
                     /locus_tag="VFL04_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7839827.1"
                     /translation="MTSPHPRIDYEAELNPEQLQAVTTTEGPLLIIAGAGSGKTRVIT
                     FRMAYMLDKGIPQHSILALTFTNKAAKEMAERVRAVTQKRLRDLTVSTFHAFGVKVLR
                     REIQLLGFKDNFSIYDEADRTQLIKDCARELGFRMEAFDAMKVGALFSDIRTQRRPWE
                     KADDAYRQLYDEYRRSLKIFNAVDFDDLIVLPIEIFELHPEVAAAYRETFRYLMVDEF
                     QDTSLIQYRMMRHLCQRNVCAVGDDDQSIYSWRGANYENLVRFERDFPGLVEIKLERN
                     YRSTSTILDAANAVIANNENRKEKALWSHGGEGGTPIQFHAPEDEGAEAEFIARTIKE
                     IRLKEKVPYGQFGVLIRTNNLTRRLEEAFLVEQLPYKVSGGTSFYGRKEIKDLISYLR
                     VIANPDDDVNLLRIINTPRRGIGKATLERLNAIARPRNASLRTAMELVRSGVLIEASS
                     ALPGEGSQPGLGIPGAELGHGLAFGGLSERAVQDISSFLDLIEEYREELLGKKRIAAK
                     VRQLIDSIDYWAYLVEENRQNEKAAKWKFHNLELLIQSIEEWEKDPDTMSPGLYEWLN
                     RVALVNRDELEDEGDGKVNLMTIHAAKGLEFDVVFIAGCEDGIIPHARSLEEGEGNLE
                     EERRLFYVAVTRARRRLFITACLRRRKQNQTVDCVPSPFLQEIPQELIAQVLAESDFT
                     EDEGAEYFGRLNAMFAQAEPAATAEEPSATAAEPAPAED"
     gene            complement(3700..4641)
                     /gene="miaA"
                     /locus_tag="VFL04_08705"
     CDS             complement(3700..4641)
                     /gene="miaA"
                     /locus_tag="VFL04_08705"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET7839828.1"
                     /translation="MSTASSNRPLVLVLCGPTASGKTALLEELFAAKGPDGKGLGGAE
                     IVSADSMQAYRGMDIGTAKPAPELLGRLPHHLIDILEPSEQYTAGDFVRLADEACETI
                     VSAGRLPVVSGGTGFYIRNFICGLPAAPKAESGLREAVAADLVRLGRAALRAELASLD
                     PASAARIHENDEYRLTRALEIVRATGRPCSDFAPGLEPRRRWRFLVAAVERPRPELFA
                     RIDARVEAMFEAGLEAEVAGLIAKGFGPEAPGMKAIGYREFLEAGEGEPKERVKLDTR
                     RYAKRQMTFFRALPGVSWIPPEASALASLLRESQDKA"
     gene            complement(4619..4810)
                     /locus_tag="VFL04_08710"
     CDS             complement(4619..4810)
                     /locus_tag="VFL04_08710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET7839829.1"
                     /translation="MIMPLEQLIEYDGNTYEFTIAVTRRAYQLAVLRTPEVEKNNGKV
                     VSMATRQVFTKQVEYRFIE"
BASE COUNT          711 a         1534 c         1767 g          891 t
ORIGIN      
        1 gggccgagct cgagccccgc atcgccgcgc tgccgaacgt gaggaccctg cgccgcgacg
       61 ccttcagcct cctgcccaaa gaggtcggaa gggtcgactg gctctgctca gacgtcatct
      121 gctacccccc cgccctcctc aagtgggtgg agctctggct cgagtcgggg ctcgcgaggc
      181 gcttcatctg cacgataaag atgcaggggg agggtttcga cagggagacg gccgaccgct
      241 tcgccgcgat acccggctcg agggtagtcc acctctggca caacaggcac gagctgacgt
      301 ggataaagac cgagtgaggg gcgcctagtc tgcgggcttg tcggagagga ctatcgcgct
      361 cgcccctacg ttgaggaact tggtggggtc gatgaggtcg cggccgaggc ggacctcgta
      421 gtggacgtgg gggcccgtgg tcaggccggt gttgccgagg atcccgatca cctggccctg
      481 cttcacctcc tgcccccgtc tgacgcggaa ggcctgaagg tgggcgtacc tcgtgtagaa
      541 gccgtgtttg tgcctgagga tgatgttgtt gccgtagccg cccgactcgt agctcaggct
      601 caccaccgtg ccgtcggcgg tcgcgacgat ggggtctccc tggccgaagg tggagatgtc
      661 gagtcccgtg tgggtgtacc actgccccgt gaaggggttc gtgttctggc cgaagtacat
      721 ggagatgtgg cctatcccgc ccttgatcgg ccagatgttc gggatgtccg acatgacggc
      781 gttctggttc cgcagaagat agccgatctg ctcgatcggg gccgaggact gctcgaggag
      841 cctgtccagc ttcgagagct cgtcggcgtc cctgccgccc gaggagtcga tgccaaggaa
      901 ggaggagagg ctcccgcccg ggcccgggga ttcggaggcc ggcacggcca ctcccacgct
      961 cgccagggaa ttggagaggg ccgtctcgaa ctgcttggcc gccgtggcca ggcgcccggt
     1021 ctgctccctg agggaatcga ggttggcgcg ggcagcggcg aggtcctggg aatcctggtc
     1081 gagctggcgt cgggaaccgg agatgtgggc cagcgagagc ccgaaggcga cgatgagcgc
     1141 gaccgaggcg agggcgatgc ccgccacccc gaagaagctc agctggacat cccgtatctt
     1201 gcgctcgtcg tgggggatga ccatgatcgt gagcttctgc ctgccgccgg agaagagcct
     1261 cgccaggccc cgcccggtgc ggtgggcggc cctgccgagc ctcgctgaga agcgtcggta
     1321 tatcgcgttc tctttcttct tgtattccct gaggaccggc aagctgtctc ctcaaatggc
     1381 gccgagaatc gttaacgtca accgtaccac tttggtgggg gccttgtcaa atgaggtttt
     1441 cgggcccccc gcgtcgcccg gggccctggc ccaggggcgg gcgccctcag tcctcggcgg
     1501 gcgccggctc ggcggccgtc gcggagggct cctccgccgt cgccgccggc tcggcctggg
     1561 cgaacatcgc gttgagcctg ccgaagtact cggccccctc gtcctcggtg aagtccgact
     1621 cggcgaggac ctgggcgatg agctcctggg gtatctcctg gaggaagggg ctcggcacgc
     1681 agtccaccgt ctggttctgc ttgcgtctgc ggagacaggc ggtgatgaag agccgccgtc
     1741 tggccctggt gacggccacg tagaagagcc ggcgctcctc ctcgaggttc ccctccccct
     1801 cctcgagcga gcgcgcgtgg gggatgatgc cgtcctcgca gcccgcgatg aagacgacgt
     1861 cgaactcgag gcccttcgcg gcgtggatgg tcatgaggtt gaccttgccg tcgccctcgt
     1921 cctcgagctc gtcgcggttc acgagggcca cccggttgag ccattcgtag aggccggggc
     1981 tcatcgtgtc ggggtccttc tcccactcct cgatggactg gatgaggagc tccaggttgt
     2041 ggaacttcca cttggccgcc ttctcgttct ggcggttctc ctcgacgagg taggcccagt
     2101 agtcgatgct gtcgatgagc tggcgcacct tcgcggcgat gcgcttcttc ccgaggagct
     2161 cctcgcggta ctcctcgatg aggtcgagga agctcgagat gtcctgcacg gcccgctcgg
     2221 agaggcctcc gaaggcgagg ccgtggccga gctcggcccc cgggatcccg aggccgggct
     2281 gggagccctc gcccgggagg gccgagctcg cctcgatgag gacccccgag cggacgagct
     2341 ccatggccgt gcggagcgag gcgttgcgtg gcctcgcgat ggcgttgagc cgctcgaggg
     2401 tcgccttgcc gatccccctc cttggagtgt tgatgatccg gaggaggttc acgtcgtcgt
     2461 cggggttggc gatgacccgc agataggaga tcaggtcctt gatctccttg cgcccgtaga
     2521 acgaggtgcc gcccgagacc ttgtagggca gctgctcgac gaggaaggcc tcctcgagcc
     2581 ggcgcgtgag gttgttcgtc ctgatcagga ccccgaactg gccgtagggc accttctcct
     2641 tgagcctgat ctccttgatg gtccgggcga tgaactcggc ctccgcgccc tcgtcctcgg
     2701 gggcgtggaa ctggatcggg gtgccgccct cccctccatg ggaccagagc gccttctcct
     2761 tgcggttctc gttgttcgcg atgacggcgt tggccgcgtc caggatggtc gaggtcgagc
     2821 ggtagttgcg ctcgagcttg atctcgacga ggccggggaa gtcgcgctcg aagcgcacga
     2881 ggttctcgta gttggcgccc cgccaggagt agatcgactg gtcgtcgtcc cccacggcgc
     2941 agacgttgcg ctggcagagg tggcgcatca tgcgatactg gatgagcgag gtgtcctgga
     3001 actcgtcgac catgaggtag cggaaggtct cgcggtaggc ggccgcgacc tcggggtgga
     3061 gctcgaagat ctcgatgggc aggacgatga ggtcgtcgaa gtccacggcg ttgaagatct
     3121 tgagggagcg gcgatactca tcgtagagct ggcggtaggc gtcgtcggcc ttctcccagg
     3181 gcctgcgctg ggtcctgatg tcggagaaga gggcccccac cttcatcgcg tcgaaggcct
     3241 ccatgcggaa gccgagctcg cgggcgcagt ccttgatgag ctgggtcctg tcggcctcgt
     3301 cgtagatgga gaagttgtcc ttgaagccga ggagctggat ctccctgcgc aggaccttca
     3361 cgccgaaggc gtggaaggtc gagacggtga ggtcgcggag ccgcttctgg gtgacagccc
     3421 gcacccgctc cgccatctcc ttcgccgcct tgttggtgaa ggtgagggcg aggatcgagt
     3481 gctgggggat gcccttgtcc agcatgtagg ccatcctgaa ggtgatcacc ctggtctttc
     3541 ccgatccggc gcccgcgatg atgaggaggg gcccctccgt ggtcgtcact gcctggagct
     3601 gctcgggatt gagctcggcc tcgtagtcta tgcggggatg ggggctggtc atggggaggg
     3661 gagtatagcc cccggggcac gggaggggca atgatcgccc taggctttgt cctggctttc
     3721 ccggagcagc gaggcgaggg ccgaggcctc gggcgggatc cagctgacgc ccggcagggc
     3781 cctgaagaag gtcatctggc gtttggcgta gcgcctcgtg tccagcttga ccctctcctt
     3841 gggctccccc tccccggctt ccaggaactc cctgtagccg atggccttca tcccgggagc
     3901 ctcgggccca aagcccttcg cgatgaggcc ggccacctcg gcctcgagcc cggcctcgaa
     3961 catggcctcg acccgcgcgt cgatcctcgc gaagagctcg ggccgggggc gctcgacggc
     4021 cgcgacgagg aagcgccagc gccgcctggg ctcaaggccg ggagcgaagt ccgagcaggg
     4081 cctgccggtg gccctgacga tctcgagggc ccgggtgagg cggtattcgt cgttctcgtg
     4141 gatcctggcg gccgaggcgg ggtcaagcga tgcgagctcg gccctcaggg ccgcccgccc
     4201 cagccggacc aggtcggcgg ccaccgcctc gcggagtccg ctctccgcct tcggggcggc
     4261 gggcaggccg cagatgaagt tgcggatata gaagcccgtg ccgcccgaga ccacgggcag
     4321 cctgccggcg cttactatcg tttcacaggc ctcgtcggcc aggcggacga aatcgcctgc
     4381 cgtgtactgc tccgagggct caaggatgtc gatcaggtgg tgggggaggc gcccgagcag
     4441 ctcgggagcg ggtttggcgg tgccgatgtc catgccgcgg taggcctgca tggagtcggc
     4501 gctcacgatc tcggccccgc ccaggccctt cccgtccggc cctttggcgg cgaagagctc
     4561 ctcgaggagg gcggttttgc cggaggccgt gggcccgcag aggaccagga cgaggggtct
     4621 attcgatgaa gcggtactca acctgcttgg tgaagacctg gcgggtggcc atggagacga
     4681 ccttgccgtt gttcttctcc acctccgggg tcctgaggac ggcgagctgg taggcgcggc
     4741 gcgtcacggc gatcgtgaac tcgtaggtgt tgccgtcgta ttcgatgagc tgttcgagtg
     4801 gcatgatcat tgcggactcc tcgggtcggg atcggggtgg aatggtatcc caagacgcca
     4861 cgccgcgcaa gtggcgccgc cgggggggcc tgccgggttc ggg
//
LOCUS       DASSQV010000493         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_241117, whole genome shotgun sequence.
ACCESSION   DASSQV010000493 DASSQV010000000
VERSION     DASSQV010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241117"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFL04_08715"
     CDS             <1..679
                     /locus_tag="VFL04_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839830.1"
                     /translation="EADGFACREMLVHVPLNVHPGARSALVIGGGDGCAVTELLTHSA
                     LERIVAVDDDEILTKAAQRFFPELAASLADPRVRFEGEDGAAFVRDSKERFDLIIASG
                     GRPGSDSFGQAFYCDCFRLLSGDGIFIQPIGSAFFPARRRELVSAAGKLKRLFPIFRL
                     YRVDSPSGEGGSRLIAFASKRHDPLEDLNPARWAELGIATRYYDPEVHRASFAMPRYI
                     REALEGA"
     gene            complement(743..2488)
                     /gene="pheT"
                     /locus_tag="VFL04_08720"
     CDS             complement(743..2488)
                     /gene="pheT"
                     /locus_tag="VFL04_08720"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7839831.1"
                     /translation="MPKIEVNERLFFSLIGRRCGRDELEELLTGAKAELDEWDLGAGA
                     AGDSAGDETRTIKIELNDTNRPDLWSTAGLARQLRVREDHRNGLKRRPDYPFFSREGD
                     MKKGTRKVIVEQSVKDSRPFLAGFVVSGKEISDALLRDMIQTQEKLCWNFGRKRRTVS
                     MGLYRTTIIQWPVRYRAVRPDSVRFVPLQWDRPMNLSEILAEHPKGKEYAFINEKLAL
                     HPLLVDSKDAVLSYPPIINSADLGAVQVGDSEMFVELTGTDLASVTLAASIVACDFAD
                     AGYKVEPVTVEYPYDTPFGREITFPYYFQTPVSVEAAKASRLLGKSLNVQAVSEALER
                     MGCETEIHGQYVTVFPPEYRNDFLHPVDAIEDVMMGKDLSFFEPERPHDFTIGRLSPI
                     EAFSRRAKGLLVGLGYQEMIYNYLGSGKDYIERMGIDGADVVRISNPMSENFEYLRNS
                     PLPSLLGSEAVSSRAAYPHRIFEVGKVAFRKASENHGVSTRQRLGFLLAHAAADYNEA
                     ASHVSTLFFYLGREYSIAEGEDPRFIAGRQASVMYKGEAVGVFGELHPRVLEAWGIAT
                     PCAAGEIDLESLLGL"
     gene            complement(2504..>3425)
                     /locus_tag="VFL04_08725"
     CDS             complement(2504..>3425)
                     /locus_tag="VFL04_08725"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET7839832.1"
                     /translation="GSWKTATFRPYNINIPASRLMPGRRNAYVEFLEGVKDKLVGLGF
                     EEFDGSLVETEFWNSDALFMPQFHSARDIHDVYYLAEPTKAKRIEEPYLSAVASTHEN
                     GGPTGSRGWNYGFDREFTKRLILRSQGTVLSARQLPTAKIPGKYFGIARCFRYDRVDA
                     THLSDFYQTEGIVLGEDVNLRTLLGMLEMFAVEVAGATEVKYVPGYFPFTEPSIEVHI
                     KHPVLGWFELGGSGIFRPEVTEPLGVTVPVAAWGIGVDRMALMALGLNDLRELFSPDL
                     ESVRMRRAKETNAGAREASVRGAGPKAKTN"
BASE COUNT          523 a         1076 c         1240 g          586 t
ORIGIN      
        1 cgaggccgac ggcttcgcct gccgggagat gctcgtgcat gttccgctca acgtccatcc
       61 gggtgcgagg tcggccctcg tcatcggggg cggggacggc tgcgcggtga ccgagctcct
      121 cacccacagc gccctcgagc gcatcgtcgc cgtggacgac gacgagatcc tcaccaaggc
      181 cgctcaacgc ttcttccccg agctcgcggc ctcgctcgcc gatccgagag taaggttcga
      241 gggcgaagac ggtgcggcct tcgtccgcga cagcaaggag cgtttcgacc tcatcatagc
      301 ctcggggggc aggcccggct cggacagctt cggccaggcc ttctactgcg actgtttccg
      361 gctcctgtcc ggtgacggca tcttcatcca gcccatcggc tcggccttct tccccgcgcg
      421 caggagggag ctcgtgagcg ccgcgggcaa gctgaagcgc ctcttcccga tcttccgcct
      481 ctatcgggtc gactcgccct cgggcgaggg aggctcccgc ctcatcgcct tcgcctcgaa
      541 acgccacgac cccctcgagg acctcaaccc cgcccgctgg gccgaactcg gcatagcgac
      601 ccgctattat gaccccgaag tgcacagggc atccttcgcg atgccccgct acatccgcga
      661 ggcgctcgaa ggggcctagg agcgcctggg gcgctcgagg gagcctagcg cctggggcgt
      721 cgaaggggcc taggcgcgcc cctcagagcc cgagcaggga ctcgaggtcg atctctcccg
      781 ccgcgcaggg cgtggcgatg ccccaggcct cgaggacgcg ggggtggagc tcgccgaaga
      841 cgcccacagc ctcgcccttg tacatgaccg aggcctggcg gccggcgatg aagcgggggt
      901 cctcgccctc ggcgatgctg tactcacggc caaggtagaa gaagagggtc gagacgtggc
      961 tggcagcctc gttgtagtcg gccgccgcgt gggcgaggag gaagcccagg cgctgccggg
     1021 tcgagacccc gtggttctcc gaggccttcc tgaaggccac cttcccgacc tcgaagatcc
     1081 ggtgcgggta ggcggcccgg ctcgacaccg cctccgagcc gaggaggctc ggcagggggg
     1141 agttgcgcag gtactcgaag ttctcggaca tggggttgga gatcctgaca acgtcggcgc
     1201 cgtcgatgcc catccgctcg atgtagtcct tgcccgagcc cagatagttg tagatcatct
     1261 cctggtagcc gaggccgacg aggaggccct tcgcgcggcg cgagaaggcc tcgatcggtg
     1321 agagcctgcc gatcgtgaag tcatggggcc gctcgggctc gaaaaaggag aggtccttgc
     1381 ccatcatgac gtcctcgatg gcgtcgacgg ggtggaggaa gtcgttgcgg tactcggggg
     1441 ggaacacggt cacgtactgg ccgtggatct cggtctcgca gcccatccgc tcgagggcct
     1501 cgctgacggc ctgcacgttg agcgactttc caagaaggcg cgaggccttg gccgcctcga
     1561 cgctcaccgg ggtctggaaa tagtagggga aggtgatctc gcggccgaag ggggtgtcgt
     1621 aggggtactc caccgtgacc ggctcgacct tatagccggc gtcggcgaag tcgcaggcga
     1681 cgatcgaggc ggcgagggtc acggaggcga ggtcggtgcc cgtgagctcc acgaacatct
     1741 cgctgtcccc cacctgcacg gcgccgaggt cggccgagtt gatgatgggg ggataggaga
     1801 gcacggcgtc cttcgagtcg acgaggaggg ggtggagggc cagcttctcg ttgatgaagg
     1861 cgtattcctt gcccttggga tgctcggcca ggatctcgct gaggttcatc ggcctgtccc
     1921 actgcagggg cacgaagcgc accgagtctg gcctcacggc gcggtagcgc accggccact
     1981 ggatgatcgt cgtgcggtag aggcccatgg agaccgtgcg gcgtttgcgg ccgaagttcc
     2041 agcagagctt ctcctgggtc tggatcatgt cccgcaggag ggcgtccgag atctccttgc
     2101 cggagaccac gaagccggcg aggaagggcc ttgagtcctt caccgactgc tcgacgatga
     2161 ccttgcgcgt gcccttcttc atgtcgccct cgcgggagaa gaaggggtag tcgggccggc
     2221 gcttgagccc gttcctgtgg tcctcgcgga cgcggagctg gcgggccagc ccggcggtcg
     2281 accagaggtc ggggcggttc gtgtcgttga gctcgatctt gatggtcctg gtctcgtctc
     2341 cggcggagtc gccggcggcg ccagctccga ggtcccactc gtcgagctcg gccttggccc
     2401 cggtgaggag ctcctcgagc tcgtcgcgtc cgcagcgccg gccgatcagg gagaagaaga
     2461 ggcgttcgtt gacttcgatc ttgggcatgt gctcgcttct cccttagttg gtcttggcct
     2521 tcgggcccgc cccgcggacc gaggcttctc tggcgccggc gttcgtctct ttcgcgcggc
     2581 gcatgcgcac gctctcgagg tcggggctga agagctcccg caggtcgttc aggccgaggg
     2641 ccatgagggc catgcggtcg accccgatgc cccaggcggc gacggggacg gtgacgccga
     2701 ggggctcggt gacctcgggc ctgaagatgc ccgagccgcc gagctcgaac cagccgagga
     2761 cggggtgctt gatgtggacc tcgatcgagg gctcggtgaa ggggaagtag ccgggcacgt
     2821 attttacctc ggtggccccc gcgacctcga cggcgaacat ctcgagcatg ccgaggaggg
     2881 tgcggaggtt gacgtcctcg ccgaggacga tgccctcggt ctgatagaag tccgagaggt
     2941 gggtcgcgtc tacccggtcg tagcggaaac agcgcgcgat gccgaagtac ttgccgggga
     3001 tcttcgcggt cggaagctgg cgggcggaaa ggacggtgcc ctggctgcgg aggatgaggc
     3061 gcttcgtgaa ctcgcggtcg aagccgtagt tccagccgcg gctgcccgtg gggccgccgt
     3121 tctcgtgggt cgaggcgacc gccgagaggt agggctcctc gatccgcttg gccttggtgg
     3181 gctcggcaag gtagtagacg tcgtggatgt cccgggccga gtggaactgg ggcatgaaga
     3241 gggcgtcgga gttccagaac tcggtctcga cgagggagcc gtcgaactcc tcgaagccga
     3301 ggccgacgag cttgtcctta acgccctcga ggaactcgac gtaggcgttg cgcctgcccg
     3361 gcatgagcct cgaggcgggg atgttgatgt tgtaggggcg gaaggtggcg gtcttccagg
     3421 atccg
//
LOCUS       DASSQV010000494         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1730894, whole genome shotgun sequence.
ACCESSION   DASSQV010000494 DASSQV010000000
VERSION     DASSQV010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730894"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="VFL04_08730"
     CDS             complement(<1..1327)
                     /locus_tag="VFL04_08730"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839833.1"
                     /translation="MSQNSDSAGQGLFAAIGRSLAAGLAARKPDAAIAAALGAALVLG
                     AALLVAFGPGAAAQGDVFATVVAGKIAERDYIADREISYVDQDATDLRVEAEQRLVLP
                     VFFVDDKPAQSALARLGQFQDLMDSLVERRLAGDTLFLNVQSQFPGLLRKDEVLALSR
                     SPFRKQALAYADSILRSFLEEGIVALPATGLERFNPDYVEIRGLRGGRPETREVPIGH
                     LVSLKNLGAAIDEEAASRRLARPLALLPGSIVRSFAVEDAFFDAQASQKRIDAVRGKV
                     ESVVRTISKGERIVHKGAIVSEADVAKLGAARGAVARIDWGAALGSIWLVGTSILLGL
                     YLLGKGLSERGLKRSGAILLSASLFAFFLVALAVARFAGPEPLELGFLLPTALVAMLA
                     AIMVGPRFAILLGLVLALIVQAAAGLDARLLLFCLLASTTATYSVRAASS"
     gene            complement(1314..>2078)
                     /locus_tag="VFL04_08735"
     CDS             complement(1314..>2078)
                     /locus_tag="VFL04_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7839834.1"
                     /translation="ESMNEGIPATPDLIIALHGDLTPEGGISGRCGDDFLDSCVQIPG
                     GFGKIFPRSKSQALYLKGLATHDLTFSVCPAGTGKTFLAMAFALRELLTKAKRKLILT
                     RPVVEAGESLGFLPGDLVAKISPYLRPLYDAMETLVPFEVVRRLEESRAIEIAPLAYM
                     RGRSLANCMVILDEAQNTTKEQMKMFLTRLGENSRAIVTGDITQIDLPRKGESGLVHS
                     LQVLGGVDDIFVSRLDARDVVRSPLVRKIIEAYESE"
BASE COUNT          324 a          656 c          805 g          293 t
ORIGIN      
        1 gggagctggc cgcgcggacc gaataggtgg ctgtggtcga ggcgaggagg cagaagagga
       61 ggaggcgggc gtcgaggccg gcggccgcct gcacgatgag ggccaagacc aggccgagga
      121 ggatcgcgaa acgcggcccc accatgatcg ccgcgagcat ggccacgagg gccgtcggga
      181 ggaggaagcc cagctcgagg ggctcggggc cggcgaacct cgccacggcg agggccacga
      241 ggaagaaggc gaagagggag gccgacagga ggatggcgcc agagcgcttg aggccccgct
      301 cggagaggcc cttgccgagg aggtagagcc ccaggaggat cgaggtcccg acgagccaga
      361 tcgagccgag ggccgcaccc cagtcgatcc tggccacggc ccctcgcgcg gcgccgagct
      421 tggcgacgtc ggcctcgctg acgatggccc ccttgtggac gatccgctcg cccttggaga
      481 tcgtccgcac gacgctctcg accttgccgc gcacggcgtc gatccgcttc tggctggcct
      541 gggcgtcgaa gaaggcgtcc tccacggcga agctgcggac gatgctcccg gggaggaggg
      601 ccaggggcct cgccagcctc cgcgaggcgg cctcctcgtc gatggccgcc cccaggttct
      661 tcaggctgac gaggtggccg atcggcacct ccctcgtctc gggcctgccg ccgcggaggc
      721 cgcggatctc gacatagtcg gggttgaagc gctcgagccc ggtcgcgggc agggccacga
      781 tgccctcctc gaggaagctg cgcaggatcg agtcggcgta ggcgagggcc tgcttacgga
      841 agggggagcg cgagagggcg aggacctcgt ccttgcggag gaggccgggg aactggctct
      901 gcacgttcag gaagagggtg tcccccgcga gcctgcgctc cacgaggctg tccatgaggt
      961 cctggaactg gccgagccgg gccagggccg actgggctgg cttgtcgtcg acgaagaaga
     1021 cggggaggac cagacgctgc tccgcctcga ccctgaggtc ggtggcgtcc tggtcgacat
     1081 agctgatctc gcggtcggcg atgtagtccc gctcggctat cttccccgcc acgaccgtgg
     1141 cgaagacgtc gccctgggca gccgccccgg gcccgaaggc cacgaggagg gcggcgccga
     1201 ggaccagggc cgcgccgagg gccgcggcga tggccgcgtc gggcttgcgc gcggcgaggc
     1261 cggccgcgag gctccgcccg atggccgcga agaggccctg cccggcggaa tcgctattct
     1321 gactcatagg cttcgatgat cttgcggacg aggggggatc ggacgacgtc gcgcgcatcg
     1381 aggcggctca cgaagatgtc gtccacgccg cccaggacct ggaggctgtg gacgaggccc
     1441 gactccccct tgcgcggaag gtctatctgg gtgatgtcgc ccgtcacgat agcccgcgag
     1501 ttctcgccga gccgggtcag aaacattttc atctgctcct tcgtggtgtt ctgcgcctcg
     1561 tccaggatga ccatgcagtt cgcgaggctc cggccgcgca tgtaggcgag gggggcgatc
     1621 tcgatggccc ggctctcctc gagcctgcgc acgacctcga agggcacgag ggtctccatc
     1681 gcgtcgtaga gggggcggag ataggggctg atcttcgcga cgaggtcgcc ggggaggaag
     1741 cccaggctct cccccgcctc gaccacgggt cgggtcagga tgagcttgcg tttggccttg
     1801 gtcaggagct cgcgcagggc gaaggccatg gcgaggaagg tcttgccggt gcccgccggg
     1861 cagacggaga aggtcaggtc gtgggtggcg aggcccttga ggtagagggc ctgggacttg
     1921 ctccgtggaa agatcttgcc gaagcccccg gggatctgga cgcaggagtc gaggaagtcg
     1981 tcgccgcagc gccccgagat ccccccttcg ggggtcaggt cgccgtggag ggcgatgatg
     2041 aggtcggggg tggccgggat gccctcgttc atggactc
//
LOCUS       DASSQV010000495         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_364654, whole genome shotgun sequence.
ACCESSION   DASSQV010000495 DASSQV010000000
VERSION     DASSQV010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364654"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFL04_08740"
     CDS             complement(<1..465)
                     /locus_tag="VFL04_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839835.1"
                     /translation="MGVSALALSLALGGCFSLGGAVEAAMGRPGQANAGGASGQGMGA
                     SGGAQGGQTAEAKPAAAYQYQFSSFYSGVWSMGWFGYGESNYKPGQGTVWTFKGSSRN
                     GEDLSFERALLRVNADKSQWWRFRLDTGKDRLLYEFLVGSDGTVLKVRYQDPD"
     gene            complement(710..>2494)
                     /locus_tag="VFL04_08745"
     CDS             complement(710..>2494)
                     /locus_tag="VFL04_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7839836.1"
                     /translation="SNTVSVSRDTEKKAVRLLADLKAFSSLVGADRNRLGLEAQVQGN
                     DKVKSYELAISALDVPGLAAGSVVRTWKDSRGVPASFSWDGSNDAKGKAPDGRYTAKL
                     SVSYLNGDSAEAQAGPFLLDSVPPSIKVSAAPLLFSPTEGSRKRSVQFSQSSVPGDDW
                     EGRLSSTDGTVVRTWTWKGQASDFVWDGTDEAGNLVKDGGYRYDVASTDAAGNKGTGS
                     VPYLTVDKRPTQVFVTASATGLSPTGAPEMRSVVFTLIVKLREGIDSWHFALVDKDGV
                     ERSVYGGQGGDIPSKIVWDGRDTAGNFTQGEYTGVFTVDYAKGDQPEARTGKILVNTE
                     GPKAEVKISPDVFSPDDYSSNNELHIGLSVKDASDIADWKFEVFEQAVVEGAAPGAAP
                     VLRSFMSWSGTGTPTPLITWDGRSSKGELVESATDYPFTFTANDVLGNSVRVDGTITV
                     DILVIREGDKLKIKVPSIVFRANFPDFVGLDQDTLARNAKVVARIAASLNKFKDYSVQ
                     IEGFANSIAKITGASPAAVAAEEQKELIPLSLGRAELVKKLLVQNGVDEKRLTTAGLG
                     SSSPVVDFQDAQNRWKNRRVEFILIKKQ"
BASE COUNT          345 a          836 c          864 g          449 t
ORIGIN      
        1 gtcggggtcc tggtagcgga ccttcaggac cgtcccgtcc gagccgacga ggaactcgta
       61 gaggagcctg tccttgccgg tgtccaggcg gaagcgccac cactggctct tgtcggcgtt
      121 cacccggagc agggcccgct cgaagctgag gtcctcgcca ttcctgctgc tgcccttgaa
      181 ggtccagacc gtgccctggc ccggcttgta gttcgattcc ccgtagccga accagcccat
      241 cgaccagacg ccggagtaga aggagctgaa ctggtactga taggccgcgg ccggcttggc
      301 ctcggccgtc tggccgccct gggccccacc cgaggctccc atgccctggc ccgaggcgcc
      361 gccagcgttg gcctgtccgg gcctgcccat cgcggcctcg accgccccgc caaggctgaa
      421 acatcccccc aaggccaggc tgagggccaa ggctgatacg cccatcgcgg ccatcctggt
      481 ccgggctctc cctgctttat tcatggcgcc aagctcctcg catggctccc gcggccctgc
      541 cccggccatc gtgtccctgg gcctccagcc cgcgagagat gctgagtgag aaggtaatat
      601 taagccgggg gaatgggaac ttcggcgcca aaatgggagg gccgcggaag cgctcgcctc
      661 cgcagccctc tgaccctccc gggtcggacc tgggcccccg aggggggcct tactgtttct
      721 tgatcaggat gaactcgacc cgccggttct tccagcggtt ctgggcgtcc tggaagtcca
      781 cgacggggct gctcgagccg agccccgcgg tcgtgagccg cttctcgtcg accccgttct
      841 gcacgaggag cttcttgacc agctcggccc tgccgaggga gagggggatg agctccttct
      901 gctcctctgc ggccacggcc gcgggcgagg ccccggtgat cttggcgatg ctgttggcga
      961 agccctcgat ctgcacggag tagtccttga acttgttgag gctcgccgcg atgcgcgcca
     1021 cgaccttcgc gttgcgcgcc agggtgtcct ggtccaggcc cacgaagtcg gggaagttgg
     1081 cgcggaagac gatggagggc accttgatct tgagcttgtc gccctccctg atcacgagga
     1141 tgtccacggt gatggtcccg tcgaccctga ccgagttgcc cagcacgtcg tttgccgtga
     1201 aggtgaaggg atagtcggta gccgactcga caagctcgcc cttcgatgag cggccgtccc
     1261 aggtgatcag cggcgtcggc gtgcccgtgc cgctccagct catgaaggag cgcaggacgg
     1321 gcgcggcgcc gggggcggcc ccctcgacga cggcctgctc gaagacctcg aacttccagt
     1381 cggctatgtc gctggcgtcc ttgaccgaga gcccgatgtg gagctcgttg ttgctcgagt
     1441 agtcgtcggg gctgaagacg tcggggctga tcttgacctc ggccttgggg ccttcggtgt
     1501 tcacgaggat cttgcccgtc ctggcctcgg gctggtcgcc cttcgcgtag tcgacggtga
     1561 agaccccggt gtactcgccc tgggtgaagt tgcccgccgt gtccctgccg tcccagacga
     1621 tcttgctggg tatgtccccg ccctggccgc cgtagacgct gcgctcgacc ccgtccttgt
     1681 cgacgagggc gaagtgccag gagtcgatgc cctcgcggag cttgacgatg agggtgaaga
     1741 ccacgctgcg catctcgggg gcaccggtgg gcgagagccc cgtggccgag gccgtcacga
     1801 agacctgggt gggccgcttg tccaccgtga ggtagggcac cgagcccgtg cccttgttgc
     1861 cggccgcgtc ggtcgaggcg acgtcgtagc ggtagcctcc gtccttgacg aggttgccgg
     1921 cctcgtcggt cccgtcccag acgaagtcgc tcgcctggcc cttccaggtc caggtcctca
     1981 ccacggtgcc gtcggtcgag ctgagcctgc cctcccagtc gtcgccgggg accgagctct
     2041 ggctgaactg cacgctgcgc ttgcggctgc cctcggtggg ggagaagagc aggggggccg
     2101 ccgagacctt gatcgagggc ggcaccgagt cgaggaggaa gggaccggcc tgggcctcgg
     2161 cgctgtcgcc gttgaggtag gagaccgaga gcttggcggt gtagcggccg tcgggggcct
     2221 tgcccttcgc gtcgttgctc ccgtcccagc tgaaggaggc gggcacgcct cggctgtcct
     2281 tccaggtcct gacgacgctt ccggcggcga ggccgggcac gtcgagggcc gagatcgcga
     2341 gctcatagga cttgaccttg tcgttgccct ggacctgggc ctcgaggccc agacggttcc
     2401 tgtcggcccc gaccaggctg ctgaaggcct tgaggtcggc gaggaggcgc acagccttct
     2461 tctcggtgtc gcgcgagacc gagaccgtgt tgga
//
LOCUS       DASSQV010000496         4341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_115823, whole genome shotgun sequence.
ACCESSION   DASSQV010000496 DASSQV010000000
VERSION     DASSQV010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115823"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1838)
                     /locus_tag="VFL04_08750"
     CDS             complement(198..1838)
                     /locus_tag="VFL04_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HET7839837.1"
                     /translation="MNSEEAPHVVETPGGLSVRYRGRLLYSERDPARLPRRTALSADP
                     GPARLLLVCSPLLWYGLPELLQRAGPGSVALCVEADPSLARLSREKAPPGLLDDPRVV
                     FLEARDAEEAVSRARSLGSFRSCSRLDLSGAASLNAGLYARMASLLSAEIESLWRSRA
                     ALTVFGRLWARNIMDNIAALPELELRAFPRFEGPAVICGAGPSLEEALPFIAANRERI
                     SVLACDTALGPMLTEGVKPDLVVCLEAQIHNLADFVPLGRTAMPLAADLSSHPASFRA
                     LHGPKHLSFVRLAPSPFLGRVAALGSALGLPWLELPPLGSVGVHAAYLARSLASGPLF
                     ATGLDFSFEVGKTHARGSPSLLAEERRLERLCRWPGQYAVSFRTRTTWAPCPALGDGR
                     RLLSDPILLSYATLLREVSGGSGPALYDIRGRGPDIGARPIGLGQAAALLGGEARSLP
                     GPALAPRPPDLRVGLASFLEEELLRLDRLTAALKGRSTHPESDLAGLLANSDYLYWSF
                     PDAERAGALPQDFLNRLLPEIEYWSWRLRALAASLPRD"
     gene            complement(1844..3436)
                     /locus_tag="VFL04_08755"
     CDS             complement(1844..3436)
                     /locus_tag="VFL04_08755"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET7839838.1"
                     /translation="MASQTPKAPAGRAVKSEADLPRRGIKNPWIYAGTIVVLAIVIVA
                     FVFVPSVGGSAGGSGFSAIEFGRYDGMSIAYTPKSYFAQQVADLDNRLRQQGMTQENY
                     QFFGFQVWRGAFERSVLRLAILEEMKKAGAIVTEGRLDERVSQLPQFQDNGKFSASLY
                     HAASLEQKLQVRADLNDELLSQQYYSDVLGLTPSAKEVAFVKEMAKETRTIDYATFPL
                     ASFPDADVAAWAQGSAGLFRLLKLSRITLSTSEADAKKLAKQVQDKAITFEDAAKGRS
                     TDSFASKGGEEGSLRFYELAADLAKKDDAEKVASLKKGELSPVLKTATGAWVFYRADE
                     ESSPADFKDAATLKTARDYMNRFERGKIEDFVAAKAKAFATEASAPKADFATAAKRAG
                     AAAKAAGPFPLNYGDLSVALYGQVVPVFKTLNTQKAPELAAASTNEKFLTAAFSLAPG
                     SVSEPIVLGDNIIVLKVKEAGSALDDATSGIELYYPYLFQQRLNVEVRDLVMKSPKLK
                     DKFSDTYFKIFKPAQPGSAAQQ"
     gene            complement(3542..>4341)
                     /locus_tag="VFL04_08760"
     CDS             complement(3542..>4341)
                     /locus_tag="VFL04_08760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET7839839.1"
                     /translation="GERQSLGQPSSSLIFIPLMIENRPIGVLTVQSYTPNAYNEKHLN
                     LLEALAPYVAIAIENSLIHDRLEFLNRTISGEKKRLEKAAQKITHLANHDMLTGLPNR
                     RLLFELLQKTFDIASRNGTKVGVIYIDLDDFKPINDRLGHMAGDRALVAIAAKLRSIL
                     RASATVARVGGDEFIAVLSNAADRNHVRAAADKILEVCAEPVIFEGSECRIGLSMGIA
                     IYPDDGETMDQLVNAADTAMYAIKRGAKNGFAFAGEIEGQAQAAGRR"
BASE COUNT          675 a         1345 c         1608 g          713 t
ORIGIN      
        1 ggcggacgcc tcgccggacg gatcaagccc tcgatcctgc gcgccctggt cgtgtcgatc
       61 ggcctcctgc tgagcctcta ctacttcatc cactgagcct tgcctctcct tccccctgcg
      121 ccgaggacca gctcaggagc atgggcggct acgaggaatg gctcgactga ggccaggaag
      181 gcacccgccg aggcggccta gtcccgcgga agagaagcgg cgagggcgcg cagcctccag
      241 ctccagtact cgatctcagg aaggagcctg ttgaggaagt cctggggcag ggcgccagcg
      301 cgctccgcgt cggggaagct ccaatagagg taatcggagt tggccaagag gccggcgagg
      361 tcggactcgg gatgggtgga ccttcccttg agcgcggcgg tcagccggtc gaggcggagc
      421 agctcctctt cgaggaagga ggcgagcccc acgcgaagat cgggggggcg cggcgccaaa
      481 gccggcccgg gcaggctccg ggcctcccct ccgaggaggg cggctgcctg accaagaccg
      541 atcggccgcg ccccgatgtc cgggcccctt ccccttatgt catacagtgc cgggcccgag
      601 cctccggaga cctcccgcaa aagcgtggcg taggagagca ggatcgggtc gctcagaagg
      661 cgacggccgt cgcccagggc agggcagggg gcccaggtag tcctcgtcct gaatgagacg
      721 gcgtattggc ccggccagcg gcagagcctc tcaagtcgcc tttcctcggc gaggagggaa
      781 gggctgcccc tggcgtgggt cttgccgacc tcgaacgaga agtccaggcc cgtcgcgaag
      841 agggggcccg aggcgaggga gcgcgccagg taggccgcat ggaccccgac cgagccgagg
      901 ggcggaagct cgagccaggg aaggccgaga gccgagccca gggccgccac ccgcccaagg
      961 aaggggctgg gcgccaggcg cacgaagctg aggtgcttcg ggccatgaag ggcccggaag
     1021 ctcgcagggt gggacgagag gtcggcggca aggggcatgg ccgtcctgcc gagggggacg
     1081 aagtcggcga ggttgtggat ctgggcctcg aggcagacga cgaggtcggg cttgaccccc
     1141 tcggtgagca tggggccgag ggccgtgtcg caggccagga ccgagatccg ttcgcggttc
     1201 gcggcgatga agggcagggc ctcctcgagc gagggcccgg ctccgcagat gacggccggg
     1261 ccctcaaagc gggggaaggc cctgagctcg agctcgggca gggcggcgat gttgtccatg
     1321 atgttgcgcg cccagagcct cccgaagacc gtcagggcgg ccctgctcct ccacagggac
     1381 tcgatctcgg ccgagagcag ggaggccatc ctcgcgtaca ggcccgcgtt gagcgacgcg
     1441 gcgcccgaga ggtcgagccg ggagcaggag cggaagctgc cgaggctccg cgccctgctc
     1501 acggcctcct ctgcgtccct ggcctcgagg aagaccaccc tcggatcgtc gagaaggccg
     1561 ggcggggcct tctcccgcga cagccgcgcg agtgaaggat cggcctcgac gcagagcgcg
     1621 acgctacctg gcccggccct ctggaggagc tcggggaggc cgtaccagag caggggggag
     1681 cagacgagga ggaggcgggc gggtccgggg tcggccgaaa gggcggtgcg ccgtggcagc
     1741 cgggcgggat cgcgctcgga gtagaggagg cggccgcggt agcggacaga aaggcccccc
     1801 ggcgtctcga cgacgtgggg ggcctcctcg ctgttcatgc cgcctattgc tgcgcggcgc
     1861 tgccgggctg ggcgggcttg aagatcttga agtaggtgtc cgagaacttg tccttgagct
     1921 tggggctctt catgacaagg tcgcggacct cgacgttgag cctctgctgg aagaggtagg
     1981 ggtagtagag ctcgatgccg ctcgtcgcgt cgtcgagggc cgaacccgcc tccttgacct
     2041 tgaggacgat gatgttgtcc ccgaggacga tcggctcgga gaccgagccg ggggcgagcg
     2101 agaaggccgc ggtcaggaac ttctcgttcg tcgaggccgc ggcgagctcg ggggccttct
     2161 gggtgttcag ggtcttgaag acgggcacca cctggccgta gagggcgacg ctgaggtcgc
     2221 cgtagttgag ggggaaggga ccggcggcct tggccgccgc gccggccctc ttggcggcgg
     2281 tggcgaaatc ggccttgggg gccgaggctt cggtggcgaa ggccttggcc ttggccgcca
     2341 cgaagtcctc gatcttgccg cgctcgaagc ggttcatgta gtcccgcgct gtcttgaggg
     2401 tcgcggcgtc cttgaagtcg gcggggctgc tctcctcgtc ggcccggtag aagacccatg
     2461 ccccggtggc ggtcttgagg acgggggaga gctcaccctt cttgagcgag gcgaccttct
     2521 cggcgtcgtc cttcttggcc aggtcggcgg cgagctcgta gaacctgagg ctcccctcct
     2581 ccccgccctt tgatgcgaag ctgtcagtgg agcggccctt cgcggcgtcc tcgaaggtga
     2641 tggccttgtc ctggacctgc ttggcgagct tcttggcgtc ggcctcgctc gtggacagcg
     2701 ttatgcggga gagcttaagc aggcggaaaa gcccagcgct gccctgggcc caggccgcga
     2761 cgtcggcgtc ggggaaggag gccaggggga aggtggcgta gtcgatggtc ctcgtctcct
     2821 tggccatctc cttgacgaat gccacctcct tcgcgctcgg ggtcagcccg aggacgtcgg
     2881 agtagtactg ctggctgagg agctcgtcgt tgaggtcggc gcggacctgg agcttctgct
     2941 cgagcgaggc cgcgtggtag agggaagccg agaacttccc gttgtcctgg aactgcggca
     3001 gctgggagac cctctcgtcg agcctgccct cggtgacgat ggcgccggcc tttttcatct
     3061 cctcgaggat cgcgaggcgg aggaccgagc gctcaaaggc gccgcgccag acctggaaac
     3121 cgaagaactg gtagttctcc tgggtcatcc cctgctggcg gaggcggttg tcaaggtcgg
     3181 ccacctgctg ggcgaagtag gacttcggcg tataggcgat gctcatcccg tcgtagcggc
     3241 cgaactcgat ggcgctgaaa cccgagccgc ccgcgctgcc gcccaccgag ggcacgaaca
     3301 caaaggcgac gatgacgatg gcgaggacca cgatcgttcc ggcgtagatc caggggttct
     3361 tgatccccct cctgggcaga tcagcttcgc tctttactgc gcgccctgcg ggggcttttg
     3421 gtgtctggga tgccatggct tccttccgta acgtatggtc tcattgggga ctggatgaac
     3481 gggcatcggg ccgacaggac ggcccaaggg cccgaacgat agcacggccc tccgagtgct
     3541 gtcaacgacg gcccgcagcc tgagcctgcc cctcgatctc ccctgcgaac gcgaagccgt
     3601 tcttggcgcc gcgcttgatc gcgtacatgg cggtgtcggc cgcgttgacc agctggtcca
     3661 tggtctcgcc gtcgtccggg tagatcgcga tccccatgga gaggccgatc ctgcactcac
     3721 tgccctcgaa gatgactggc tcggcgcaga cctcgaggat cttgtccgcc gcggccctga
     3781 catggttccg gtcggcggca ttcgacagca cggcgatgaa ctcgtcgccg ccgaccctcg
     3841 cgacggtggc cgatgcccgg aggatcgacc tgagctttgc cgcgatggcc accagggccc
     3901 ggtcgcccgc catgtggccc aggcggtcgt tgatgggctt gaagtcgtca aggtcgatgt
     3961 agatgacccc gaccttggtg ccgttccgcg aggcgatgtc gaaggtcttc tggaggagct
     4021 cgaagaggag gcggcggttg ggcaggccgg tgagcatgtc gtggttggcg aggtgggtga
     4081 tcttctgcgc ggccttctcc agcctcttct tctcgcccga tatggtgcgg ttcaggaact
     4141 cgaggcggtc atggatcagg ctgttctcga tggcgatcgc gacatagggc gccagggcct
     4201 cgaggagatt caggtgcttc tcgttgtagg cgttgggggt gtagctctgg accgtgagca
     4261 ccccgatggg ccggttctcg atcatcagcg gtatgaagat cagggagctc gagggctgcc
     4321 caaggctctg ccgctcgccc t
//
LOCUS       DASSQV010000497         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_863447, whole genome shotgun sequence.
ACCESSION   DASSQV010000497 DASSQV010000000
VERSION     DASSQV010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863447"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..276)
                     /locus_tag="VFL04_08765"
     tRNA            complement(204..276)
                     /locus_tag="VFL04_08765"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(241..243),aa:Ala,seq:ggc)
     gene            complement(386..766)
                     /locus_tag="VFL04_08770"
     CDS             complement(386..766)
                     /locus_tag="VFL04_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839840.1"
                     /translation="MKKFWIAALVLLAAFAISSCTTFKASGLTVNKGPVTFTVLGNFQ
                     TDITVSEFLGAPAGAKLLNITANATEQAIADEIDKQIKAKGGTGVINLTIRHKASFFD
                     ILLSAITLSIYSPGTIEISGTVVK"
     gene            878..>2130
                     /locus_tag="VFL04_08775"
     CDS             878..>2130
                     /locus_tag="VFL04_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET7839841.1"
                     /translation="MKEPGYALALCALGLEKVCAAELSRLGLEVVGREPGRVRFALGA
                     GSGKAGPGQAGLSAGRILARANICLRTAERILIEAASFRATDFDALFEGAREVPWELF
                     FSREDRLVIERVRLHRSVLAAQTSVQAIVHKAIYERLGKAFGLSRLPESGRERSLRVY
                     IEDDEVLLGLDSSGEALHKRGYRQATGEAPLKETVAAGVLLLAGWNRRLPLLDPFCGS
                     GTILIEAGLFAQDRAPGLGRSFGFEDMPSVSRADLAAELEAARSRVRTDADFRIEGSD
                     LSPQALEVARANVRTAGLAGRIELSQAKAEEARPGYEAGILLCNPPYGERLGTVEESE
                     ELYRSLGRTAAAFSGWGLGYVTNRRDFGEFFGERSVSMHKIMNGAEEQWFHWYPATRP
                     APSERATRPAPSERATRPAPADRSTR"
BASE COUNT          338 a          717 c          731 g          344 t
ORIGIN      
        1 aggaacggaa ccagggcttc ggctgacgca cgagtaaacc tcccgtgaag cacgggtgag
       61 ccggaaggct tactcttggc ccctcccctg gtgacgcagg ggaggggttt ttgcgttacc
      121 ctgcacgata ccctgcacgg ctggcatttc cgggcagtcg cctaggtcgc ggaagtgcct
      181 tgcttcctag gcgtttccgt cagtggaggt gaggggattt gaacccctga cctcttgaat
      241 gccattcaag cgctctacca actgagctac acccccgaca cgaggcgggg gcatcctagc
      301 cgagagggga gggacatgtc aagcggtgaa gggtatttgc gcgactaaag cccttgccgc
      361 cgaacctccc gagctcggcc gcgggctatt tgacgaccgt gcccgagatc tcgatggtgc
      421 cgggggagta gatggacaaa gtgatcgcgg acaggaggat gtcaaagaag ctggccttgt
      481 gcctgatcgt caggttgatg accccggtgc ctcccttggc cttgatctgc ttatcgatct
      541 cgtcggcgat cgcctgctcg gtggcgttcg cggtgatgtt gaggagcttc gccccggcgg
      601 gagcgccgag gaactcgctc acagtgatgt cggtctggaa gttgcccagg accgtgaagg
      661 tgaccgggcc cttgttgacg gtcaggcccg aggccttgaa ggtcgtgcat gaggagatgg
      721 cgaaggccgc gaggaggacg agcgctgcga tccagaactt cttcatggtc ccatcctttg
      781 tagggcgatt ctgctctgta gtataggcgc atcgggaacg ccattggtag tgcctcgaag
      841 cttgttgagg agccgcggtt caggccatac tgaagccgtg aaagaaccag gatacgccct
      901 cgccctctgc gcgctcgggc tcgaaaaggt gtgcgcggcc gagctctcgc gcttgggcct
      961 cgaggtcgtc ggacgggagc cggggagggt gcgcttcgcc ctcggggcag ggtccggcaa
     1021 ggcggggcca ggccaggccg ggctcagcgc gggcaggatc ctggccaggg ccaacatctg
     1081 cctcaggacg gccgagcgca tcctcatcga ggccgcctcc ttccgggcca ccgacttcga
     1141 cgccctcttc gagggggcga gggaggtccc ctgggagctc ttcttctccc gggaggaccg
     1201 gctggtcatc gagcgggtca ggctccaccg ctcggtcctc gccgcccaga cctcggtcca
     1261 ggccatcgtg cacaaggcca tctacgagcg gctcgggaag gccttcgggc tcagccgcct
     1321 ccccgagagc gggagggagc ggagcctcag ggtctatatc gaggacgacg aggtcctcct
     1381 cggcctcgac agctcgggcg aggccctgca caagcggggc tatcggcagg ccacgggcga
     1441 ggccccgctc aaggagaccg tcgccgccgg cgtcctcctc ctcgccggct ggaaccggcg
     1501 ccttcccctc ctcgacccct tctgcggctc gggcacgatc ctcatcgagg ccgggctctt
     1561 cgcccaggac cgggccccgg gcctgggccg ctccttcggc ttcgaggaca tgccctcggt
     1621 ctcgagagca gacctcgccg ccgagctcga ggcggcccgc tcccgcgtca ggaccgacgc
     1681 cgacttccgg atcgagggga gcgacctgag cccccaggcc ctcgaggtcg cgcgcgccaa
     1741 cgtcaggacc gccggcctcg ccggccgcat cgagctcagc caggccaagg ccgaggaggc
     1801 cagacccggc tacgaggcgg ggatcctgct ctgcaacccg ccctacggcg agcgactcgg
     1861 gaccgtcgag gagtccgagg agctctaccg cagcctcggc cggaccgccg ccgccttctc
     1921 gggctggggc ctcggctacg tcacgaaccg cagggacttc ggcgagttct tcggcgagcg
     1981 ctcggtgtcc atgcacaaga tcatgaacgg ggccgaggag cagtggttcc actggtaccc
     2041 cgccacgcgc ccggcgccct ccgagagggc cacgcgcccg gcgccgtccg aaagggccac
     2101 gcgcccggct cccgccgatc gctccacccg
//
LOCUS       DASSQV010000498         3629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_491687, whole genome shotgun sequence.
ACCESSION   DASSQV010000498 DASSQV010000000
VERSION     DASSQV010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491687"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..623)
                     /locus_tag="VFL04_08780"
     CDS             complement(9..623)
                     /locus_tag="VFL04_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HET7839842.1"
                     /translation="MASQAFQLRTYAFLDSMQPQYAAFLGSELDGDVPLAGMAELWLE
                     LSPGSEVYNLLDAALKTTGARPGLQMVEREFGIIELHSEDVDSVKDAGRTILDACGLD
                     EGARLRPQLVSAKVIHRVNPYQAQQINKNRKGNLLIPGRCLLVMEVEPAAYVVQAANA
                     AEKAADVTLVHFNPFGRFGRLFLAGTEADIAVAQDAATEALGRP"
     gene            complement(640..912)
                     /locus_tag="VFL04_08785"
     CDS             complement(640..912)
                     /locus_tag="VFL04_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HET7839843.1"
                     /translation="MIETRGLVAAIEATDSMSKSAEVQVLGLKEIGGGLVSVYVQGEV
                     GAIKAAIDAGAASAKQVGEIVSVHIISRPNDDTVKVLNELLKLKLA"
     gene            complement(924..1205)
                     /locus_tag="VFL04_08790"
     CDS             complement(924..1205)
                     /locus_tag="VFL04_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET7839844.1"
                     /translation="MRICRVIGKAQATVKHQSLEGTRLLVLRDLEAKAGSAGAPFLAV
                     DAVGAGEGETVAVCTGGAAARAIGLREAPVDAVVVGILDTITIEGEERK"
     gene            complement(1214..2602)
                     /locus_tag="VFL04_08795"
     CDS             complement(1214..2602)
                     /locus_tag="VFL04_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7839845.1"
                     /translation="MREIITGTDIDKASRAGQAELRVGAEAVITGVARERAEKLGIRI
                     VAGPGGQAQGGQAFMPAFAGASARAGGSAAAGAPTGASRPSYDLEAWRKKFPILKKAV
                     HVANCSQSPQSDQTRAAAMAYLDNWDGMGMDWGRWMEEIALAKAEFAKLINAKPSEIA
                     IGTSVSELTSAVGSSLPLNTGRMKVVTTEAEFPTVGNIWNAFRKFDFQPEFLKVRNGT
                     VDLSEYDRLLDERTLIASIFDVYYYNGFKQDLAAIIPKIHAVGALAYVDAYQGIGSHP
                     IDVKALDIDILATGNLKYLCGIPGVAFMYVKEELVPHLEPAFTGWFGQKNPFAFKLDL
                     DFAMDARRFDNGTPPVLTAYIARAGMAIINEVGPAAIQAWTDVLSRRCIEGAQARGMD
                     IASPLDIARKAPTTAIRVGPDSHHVELALREAGIIASARGDVIRIAPHFFTRLEDIDY
                     VLDSLEKVLKRR"
     gene            complement(2764..3270)
                     /locus_tag="VFL04_08800"
     CDS             complement(2764..3270)
                     /locus_tag="VFL04_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET7839846.1"
                     /translation="MSDTGHQMPTGMRNVRLEDTDLDEIDLKILGILEEDARTPYSTL
                     AEMVGLSRVSVKERVNELKRRGVIERFTIQIPAKHIGKPLPVFFDLKFQPELLEAAAL
                     VITDHPDIVIVYQMSGINALHVHGFFKDIDEVSTFVNSFVSKIPGVQAIASEFLFKRY
                     KADRMLLV"
BASE COUNT          563 a         1151 c         1247 g          668 t
ORIGIN      
        1 cgccatgctc agggcctgcc caaggcctcg gtcgcggcgt cctgggccac ggcgatgtcg
       61 gcctcggtgc ccgcgaggaa gagccgcccg aagcgcccga aggggttgaa atgcaccagg
      121 gtgacgtcgg ccgccttctc ggcagcgttg gccgcctgca ccacataggc cgcaggctcg
      181 acctccatga cgaggaggca gcggccgggg atgaggaggt tgcccttgcg gttcttgttg
      241 atctgctggg cctggtaggg gttcacccga tggatgacct tggccgacac gagctggggc
      301 ctgagcctgg cgccctcgtc gaggccgcag gcgtccagga tggtcctgcc ggcgtccttg
      361 accgagtcga cgtcctcgga gtggagctcg atgatgccga actcgcgctc gaccatctgg
      421 agccccggcc tcgcgcccgt ggtcttgagg gcggcgtcga ggaggttata gacctcgctc
      481 cccggcgaca gctcgagcca gagctcggcc atccccgcga gggggacgtc gccgtcgagc
      541 tccgacccga ggaaggccgc gtactgcggc tgcatgctgt cgaggaaggc gtaggtccgc
      601 agctggaaag cctgggaggc catggcgcgg ccccccgtct caggcgagct tcagcttgag
      661 gagctcgttc agcaccttca ccgtgtcgtc gttgggcctc gagatgatgt ggacggagac
      721 gatctcgccg acctgcttgg ccgaggccgc gccggcgtcg atcgcggcct tgatggcccc
      781 gacctcgccc tggacgtaga ccgacacgag cccgccgccg atctccttga gcccgaggac
      841 ctggacctcg gccgacttgg acatggaatc ggtggcctcg atcgccgcga ccaagcccct
      901 ggtttcgatc attcccaatg ccttcatttc ctctcctccc cttcgatggt tatggtgtcc
      961 agtatcccga ccacgacggc gtcgacgggg gcctcgcgga ggccgatggc cctcgccgcc
     1021 gcccctcccg tgcagaccgc cacggtctcg ccctcacccg ccccgacggc gtcgacggcc
     1081 aggaagggcg cgcccgcgct cccggccttg gcctcgaggt cgcggaggac gaggagcctc
     1141 gtcccctcca ggctctggtg cttcaccgtg gcctgggcct tgccgatgac gcggcaaatc
     1201 ctcataccgc ctcctatcgc cgcttcagca ccttctccag cgagtcgagg acgtagtcga
     1261 tgtcctcgag cctcgtgaag aagtgcggcg cgatgcgtat gacgtcgccc cgggccgagg
     1321 cgatgatccc ggcctcgcgc agggcgagct cgacatggtg ggagtcaggc ccgaccctga
     1381 tggccgtcgt cggggccttg cgcgctatgt cgaggggact cgcgatgtcc atgcccctgg
     1441 cctgggcccc ctcgatgcag cgccttgaga ggacgtcggt ccaggcctgg atggccgccg
     1501 gccccacctc gttgatgatg gccatgccgg ccctcgcgat gtaggcggtc aggacgggcg
     1561 gggtgccgtt gtcgaagcgg cgggcgtcca tcgcgaagtc gaggtcgagc ttgaaggcga
     1621 aggggttctt ctggccgaac cagccggtga aggcgggctc gaggtggggg accagctcct
     1681 ccttcacgta catgaaggcc acgccgggga tcccgcagag gtacttgagg ttgcccgtgg
     1741 cgaggatgtc gatgtcgagg gccttcacgt cgatggggtg gctgccgatg ccctggtagg
     1801 cgtcgacgta ggcgagggcc ccgacggcgt ggatcttggg gatgatggcg gcgaggtcct
     1861 gcttgaagcc gttgtaatag tagacgtcga agatcgaggc gatgagggtc cgctcgtcga
     1921 ggagcctgtc gtactcggag aggtccaccg tgccgttcct caccttgagg aactccggct
     1981 ggaagtcgaa cttgcggaag gcgttccaga tgttgccgac cgtggggaac tcggcctcgg
     2041 tggtgaccac cttcatccgc cccgtgttga ggggaagcga ggagccgacc gcgctcgtga
     2101 gctcggacac cgaggtgccg atggcgatct ccgagggctt ggcgttgatg agcttcgcga
     2161 actcggcctt ggcgagggcg atctcctcca tccagcggcc ccagtccatg cccatgccgt
     2221 cccagttgtc gaggtaggcc atggcggcgg ccctggtctg gtcgctctgg ggcgactggg
     2281 agcagttggc gacgtggacg gcctttttga ggatggggaa cttcttgcgc caggcctcga
     2341 ggtcgtagga gggtcggctg gcgccggtcg gggcgcccgc tgcggccgag ccgccggccc
     2401 tggccgatgc gccggcgaag gcgggcatga aggcctggcc cccctgggcc tggccgccgg
     2461 ggccggccac gatgcggatc ccgagctttt ccgcccgctc ccgggccacg cccgtgatga
     2521 ccgcctcggc tcccacgcgg agctcggcct ggccagccct gcttgccttg tctatgtcag
     2581 ttccggtgat aatctctctc atccagatca cctcgctcgg cccattgcct cggagagtat
     2641 acgacaggat ggcgaaaacg caagcgcatt aagtcctgac tttacaatat gtaggcaaaa
     2701 ccgcgatgag gccttatttt tgtcaaggca gaacggcgcg cgccccccgt ttcgggggcc
     2761 cggctagacc aggagcatgc ggtcagcctt gtagcgcttg aagaggaact ccgaggcgat
     2821 ggcctggacc ccggggatct tggacacgaa ggaattgacg aaggtcgata cctcgtcgat
     2881 gtccttgaag aagccgtgga cgtggagggc attgatcccg ctcatctggt agacgatcac
     2941 gatgtcgggg tggtcggtga tgacgagggc ggcagcctcc aggagctcgg gctggaactt
     3001 caggtcgaag aagacgggga gcggcttgcc gatgtgcttg gccggtatct ggatcgtgaa
     3061 gcgctcgatg accccgcggc gcttgagctc gttgaccctc tccttcacgc tcacccggga
     3121 caggcccacc atctcggcga gggtggaata gggggtccgg gcgtcctcct cgaggatccc
     3181 gaggatcttc aggtcgatct cgtcgaggtc ggtgtcctcg agcctcacgt tcctcatgcc
     3241 cgtgggcatc tggtggccgg tatcggacat ttcgtgctct ccttcgccct ggacgggcgc
     3301 catgccggga cggccgggcc cgcgcctgcg gacccggggg ccaatcctga caatcagcat
     3361 agcatactaa accttttccc gactttttga aaggaatatt gagaattgac ttatcatttt
     3421 gtcgggttta tagtggctta ctcttacggt actgcatcaa cggaggtgcg ttcgatgaaa
     3481 gagatacgac ttcacggacg cggaggaatg ggcaccgtca aagcggccga ggtcatcgtc
     3541 catgcgatgg tcgcgaaggg cgggtccggc aactcgatcc ccttcttcgg cttcgagcgc
     3601 cagggcgcgc cggtcacctc attcatccg
//
LOCUS       DASSQV010000499         3223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1732521, whole genome shotgun sequence.
ACCESSION   DASSQV010000499 DASSQV010000000
VERSION     DASSQV010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732521"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFL04_08805"
     CDS             complement(<1..564)
                     /locus_tag="VFL04_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7839847.1"
                     /translation="MGSILLALCAIGIWSSLAVLSTKVSHLPPLFSVGLALCVGGLAG
                     AFRARDWRVPLLTFAIGVGGIFGYHFLLFTAFRSAPAVEANLLNYLWPLLIVVLSPLF
                     LPGLALGLRHVAGAILGLAGAVMIVSGGRLSPDLSHLGGYLLAAAAALVWACYSLLTK
                     RVRPFSTGAVGGFCLASGLLSLCSFGLE"
     gene            707..1987
                     /locus_tag="VFL04_08810"
     CDS             707..1987
                     /locus_tag="VFL04_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HET7839848.1"
                     /translation="MESYAVVIVGSGPAGLGAAFELLARSPGLSVLMLDKNEFSSGGL
                     RNDCKMNFTWPIGFPEPCWSGPQAEAYLGRVEAFLRPRIMEKRNVGVYARRAEKIGVR
                     LLEIRQAHLGTDGGLELIKSLVGRLQELGATLSLGERAIAAEAKAKVLRTDRRELGYR
                     NLIIAPGRGGFAFLQELMAGAGVQFTDNVVDVGIRLETGEERYPIVRDYYDPKFLFPK
                     KTRTFCTNSRAAHVVQEKYEDKDGGVWYSVNGHAWSGSRPPNGLVNFAMLKTVTLTEP
                     LASGQAYARMLGVQAALLGGGRPIMQRVGDFRLGKRSTRESFSGDLYDFEPTLPSATP
                     GDIGLMAPAKVMRALWNAMKLLDSIVPGLLHPSTIMYYPEIKLYANKPVFMDAHFRAA
                     PGLYLIGDGAGTSRGITAAWASGMRAADGVLASL"
     gene            2002..3120
                     /locus_tag="VFL04_08815"
     CDS             2002..3120
                     /locus_tag="VFL04_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET7839849.1"
                     /translation="MNRTEIARAKAHLKEEREAAFLYGRLSAAEPNEGIASIYARLAE
                     TEARHAAFWEQKLGEAGIKPGAFRPSLKARLSARMGDRFGPASVISALAATEREAAGT
                     YQAEPGAESLVADERSHARVFSLMAKATGGMAGGEVARFEGRHRSGGNALRAGTLGAN
                     DGLLSVYSLVMGVAGAGVGKDQILLTGIAGLLAGALSMALGEWISVQSSRELYEHQVE
                     IEREELSDNPEEEAEELALIYMAKGLDPEQARRTAGSIIDKGGEAALDTLVREELAID
                     RSELGGSAWEAAFTSFGLFALGAAIPVAPFLFFSGAAGIAGSSLASALGLFGIGALTT
                     LMTGKNPVIQGLRQVAIGIAAAACTYGVGRLVGVAIAG"
BASE COUNT          553 a         1108 c         1130 g          432 t
ORIGIN      
        1 ctcgaggccg aagctgcaga gcgagaggag gccggaggcg agacagaagc cccccacggc
       61 cccggtggag aagggcctca cccgcttcgt gaggagggaa tagcaggccc agacgagggc
      121 cgcggcagcc gcgaggaggt agccgcccag gtgggagagg tcgggcgaga gcctgccgcc
      181 cgagactatc atgacagccc ccgcaagtcc gaggatggcc cccgccacgt gcctgaggcc
      241 aagggccagg cccggaagga agagcgggct caggaccacg atgaggaggg gccagaggta
      301 gttgaggagg ttcgcctcca cggcgggcgc cgagcggaag gcggtgaaga ggaggaagtg
      361 gtagccgaag atccccccga cgccgatggc gaaggtcagg aggggcaccc tccagtccct
      421 cgccctgaag gcgccagcga ggcccccgac gcagagcgcg agcccgaccg agaacagggg
      481 gggcaggtgg gacaccttgg tgctcaggac cgcgaggctg ctccagatgc ctattgcgca
      541 caacgcgagc aagatcgatc ccatcgacgc tccattcccc ggggatggcg attggggggc
      601 agccaactat aaccccggcg gcggggcggc acaaggcgag ccggccgggt tcgggcccgg
      661 acccgctggc gtctcgggcc gggatcagat atcctctccc aacgtgatgg aaagctacgc
      721 tgtcgtcata gtcggctcgg gaccggcggg tctcggggca gccttcgagc tcctggcccg
      781 aagcccaggt ctgagcgtcc tcatgctcga caagaacgag ttctcctcgg gcgggctgcg
      841 gaacgactgc aagatgaact tcacctggcc cataggcttc cccgagccct gctggtcggg
      901 gccccaagcc gaggcctatc ttggccgggt cgaggcattc cttcgcccca ggatcatgga
      961 gaagcggaac gtcggggtct atgctcgccg ggccgagaag atcggcgtcc gcctcctcga
     1021 gatacgccag gcccatctcg ggaccgacgg cggcctcgag ctcataaaga gcctggtggg
     1081 gcgtctgcag gagctgggcg cgaccctctc tctgggggaa agggccatcg cggccgaggc
     1141 caaagccaag gtactcagga ctgacaggcg ggagctcggc taccggaacc tcatcatcgc
     1201 gccggggcgg gggggcttcg ccttcctcca ggagctgatg gccggggccg gggtccagtt
     1261 caccgacaac gtcgtcgacg tcgggatcag gctggagacg ggcgaggagc gctatcccat
     1321 cgtccgcgac tactacgacc ccaagttcct cttccccaag aagacaagga ccttctgcac
     1381 gaattcccgg gccgcccacg tggtgcagga gaagtacgag gacaaggacg gaggggtctg
     1441 gtacagcgtc aacggccacg cctggtcggg ctcgcggccg cccaacggcc tcgtcaactt
     1501 cgccatgctc aagaccgtca ccctcaccga gcccctggcc tcgggccagg cctacgcccg
     1561 catgctcggg gtccaggccg ccctcctcgg cggaggcagg ccgatcatgc agcgcgtggg
     1621 cgatttccgc ctcggcaaac gctccacccg cgagtccttc tcgggcgacc tctacgactt
     1681 cgagcccacc cttccctcgg ccaccccggg ggacatcggc ctcatggccc cggccaaggt
     1741 gatgcgcgcg ctgtggaacg ccatgaagct cctcgacagc atcgtgcccg ggctcctgca
     1801 tcccagcacg atcatgtact atcccgagat caagctctat gcgaacaagc cggtcttcat
     1861 ggacgcgcac ttccgcgccg ctccgggcct ctacctgatc ggggacgggg ccggcacgag
     1921 ccgcggcatc acggcggcat gggcctcggg catgagggct gcggacggcg tcctcgcgtc
     1981 cctttgagga ggatcgacac gatgaacagg accgagatcg caagggccaa ggcccacctc
     2041 aaggaggagc gcgaggccgc cttcctctac ggccggctct cggcggccga gcccaacgag
     2101 ggcatcgcct cgatctatgc ccggctggcc gagaccgagg ccagacacgc ggccttctgg
     2161 gagcagaagc tcggcgaggc ggggatcaag ccgggagcct tcaggcccag cctcaaggcc
     2221 aggctctcgg ccaggatggg cgaccgcttc ggccccgcct cggtcatcag cgccctcgcc
     2281 gccacggagc gcgaggcggc ggggacctac caggccgagc ccggggccga atcacttgtc
     2341 gccgacgagc gctcgcacgc cagggtcttc agcctcatgg ccaaggccac ggggggcatg
     2401 gccggcggag aggtcgcgcg tttcgagggt aggcaccgct cgggcggcaa cgccctcagg
     2461 gccgggaccc tgggagccaa cgacggcctc ctctcggtct acagcctcgt catgggcgtc
     2521 gcgggggccg gggtcgggaa ggaccagatc ctgcttacgg gcatcgccgg cctcctcgcc
     2581 ggcgcccttt ccatggccct gggcgagtgg atctcggtgc agagctcgag ggagctctac
     2641 gagcaccagg tcgagatcga gcgcgaggag ctcagcgaca accccgagga ggaggccgag
     2701 gagctcgccc tcatctacat ggccaagggc ctcgaccccg agcaggcgcg gaggacggcc
     2761 ggaagcatca tcgacaaggg cggggaagcc gccctcgaca ccctcgtccg cgaggagctc
     2821 gcgatcgacc gctccgagct gggcggctcg gcgtgggagg cggccttcac ctccttcggc
     2881 ctcttcgccc tcggcgcggc gatacccgtc gctcccttcc tcttcttctc cggagccgcg
     2941 ggcatagccg gcagctcgct cgcgagcgcc ctgggcctct tcggcattgg ggccctgacc
     3001 accctcatga cggggaagaa ccccgtgatc cagggcctga ggcaggtggc gatagggatc
     3061 gccgccgcgg cctgcaccta cggagtgggc cgcctcgtgg gcgtcgcgat agccggctag
     3121 tacctgacct ccattaggca gagcccgaag gccccgagca tgccgggggc ctggctcctg
     3181 tcgcgggcgg ccagaaggcc gcgcagctcc tcggggccga gcc
//
LOCUS       DASSQV010000500         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1361910, whole genome shotgun sequence.
ACCESSION   DASSQV010000500 DASSQV010000000
VERSION     DASSQV010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361910"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VFL04_08820"
     CDS             complement(<1..420)
                     /locus_tag="VFL04_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7839850.1"
                     /translation="MISFEGIGRRYGEVRAVSGLYLEIRDRELCVLVGPSGAGKSTVL
                     RMINRLVEPSEGRILLDGVELGTMRVEALRRGIGYVIQGVGLFPHLTVSANVAVVPQL
                     LGWDRRRTAARVDEMLELVGLDSAVYGGRYPAELSGGE"
     gene            complement(417..1649)
                     /locus_tag="VFL04_08825"
     CDS             complement(417..1649)
                     /locus_tag="VFL04_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7839851.1"
                     /translation="MAVLASAAAVFLPFWRLRPNRIARGQGILLASAEPLGFALLLGL
                     WLAFVVLGSRQGQLRSRLAALASLATAVLGLALTAHASAILAGSSPIARVSLGPGFWF
                     LCVASYAGFVLAERGTAAAHGRGPDAGGRAPKDAEGPAPGAAGGPARAAFRLACAAAF
                     AGLAALLLLSGSLDALSITREWRAQSQVFALETKRHLALTASALLFGGIIGAAIGIAA
                     PRRTGDRGPLFVFLNVVQTMPSLALFGILIIPLAALAERFPVLREWGIGGIGPAPALI
                     ALSLYAALPIARNTWIGLEGVPPSARDAGRGMGMGALQIFLRVELPLALPVALAGLRT
                     AAVQAVGNVAIAALIGAGGLGVFIFQGLGQFAMDMVLAGTVPLIALALAADAGLGWLS
                     RALTPRGLKVGAAAGEGA"
     gene            complement(1784..2704)
                     /locus_tag="VFL04_08830"
     CDS             complement(1784..2704)
                     /locus_tag="VFL04_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7839852.1"
                     /translation="MKLKALVFFAAALIIVGAASSQSMSRPLVVGSKIDTEGSILGKM
                     IVKLLAANGVPVTDKVGFGTTDLVRKAIVAKELDLYIEYTGNGAFFFQGTDPSVWQDE
                     RKGYETVKGLDMQRNSIVWLTPAPANNTWAIAVRRDLAKKEGLASLEDFARYAARGGA
                     VKLAASEEFISSPAALPAFQKAYGFSLGKDQLLSFSGGNTALTEQAASQGSNGVNFAM
                     AYGTDGSLAALDLLVLADTKKVQPIYEPTPIIRKDALDRFPAVAGLLEPLFKGLDLET
                     LQGLNARAAVNGESPDAVAASYLKDKGFLK"
     gene            complement(2873..>3269)
                     /locus_tag="VFL04_08835"
     CDS             complement(2873..>3269)
                     /locus_tag="VFL04_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839853.1"
                     /translation="DLALLVVGALAYSAGLAFLLVRIAIAAGTKEEGALCFARLVRER
                     EGWSYGIGGILCLDGEGLSFLRRGRRIGLWRAASPTRIAEIQGARAAFVLGRVELRPY
                     LRFETDQGQLTIDAIVPAELSRRLGAPRP"
BASE COUNT          501 a         1101 c         1258 g          409 t
ORIGIN      
        1 ctcgccgccg gagagctcgg ccggatagcg cccgccgtag acggcgctgt ccagccccac
       61 gagctcgagc atctcgtcga cccgcgccgc cgtgcggcgc cggtcccagc ccaggagctg
      121 ggggacgacc gccacgttgg ccgacaccgt caggtggggg aagaggccca cgccctggat
      181 cacgtagccg atcccccgcc tcagggcctc gaccctcatg gtcccgagct cgaccccgtc
      241 gaggaggatc ctgccctcgc tcggctcgac gaggcggttt atcatccgca ggacggtgga
      301 cttgccagcg cccgatgggc ccacgaggac gcagagttcc ctgtcgcgga tctcgaggta
      361 gaggcccgag acggccctca cctcgccgta gcgccgaccg atgccctcga agctgatcat
      421 gctccctccc cagccgcggc gccgaccttg agcccccttg gcgtaagcgc ccgtgagagc
      481 catcccaagc ccgcgtcggc ggcgagggcc agggcgatga ggggaaccgt ccccgcgagc
      541 accatgtcca tcgcgaactg gcccagaccc tggaagatga agaccccgag gcctccggcc
      601 cctatgaggg cggctatcgc cacgttgccg acggcctgga cggccgccgt cctgagcccg
      661 gccagggcca cgggcagggc aagggggagc tcgaccctga ggaagatctg cagggcgccc
      721 atgcccatgc ccctgccggc gtcgcgggcc gaggggggga ccccctcgag gccgatccag
      781 gtgttgcgcg cgatgggcag ggcggcgtag aggctgaggg cgatgagggc cggggcgggc
      841 ccgatgccgc cgatgcccca ttcgcgcagg acggggaagc gctcggcgag ggcggcgagg
      901 gggatgatga ggatgccgaa gagggccaga ctcggcatgg tctggacgac gttgaggaag
      961 acgaagagag gcccgcggtc cccggtcctg cgcggggccg cgatcccgat ggccgccccg
     1021 atgatgccgc cgaagagaag ggccgaggcc gtgagggcga ggtggcgctt cgtctcgagg
     1081 gcgaagacct ggctctgggc cctccactcg cgggtgatcg agagggcatc gagcgagccc
     1141 gaaagcagga ggagggcagc gaggccggcg aaggcggcgg cgcatgcgag cctgaatgcc
     1201 gccctcgccg ggcctccggc ggcgcccggc gccgggcctt cggcatcctt gggcgcccgc
     1261 cctccggcgt cgggcccgcg gccgtgggca gccgccgtcc cccgctcggc caggacgaaa
     1321 cccgcatagg aggcgacgca gaggaaccag aaccccgggc ccaggctcac ccgcgcgatg
     1381 gggctcgagc ccgcgaggat ggcggaggca tgggcggtca gggccaagcc gaggaccgct
     1441 gtggccagag aggccaacgc ggcgagcctc gagcgcagct gcccctgccg cgagccgagg
     1501 acgacaaagg ccagccagag cccgaggagg agggcgaagc ccaggggctc ggccgacgcg
     1561 aggaggatgc cctgtcccct cgctatccgg ttgggccgca ggcgccagaa tggcaggaag
     1621 acggccgccg ccgaggccag gacggccacg gcggccgtgg cggcgcggag ggctggagcc
     1681 gcggccgcgg cgcggacagg gggagccgct gctgggacag cgcgagccgc ggaggcggcg
     1741 cggacgcccg gggcaaggcg cgttgagcgc gccccgcccg gcctcacttg aggaagccct
     1801 tgtccttcag atagctcgcg gccacggcgt cggggctctc gccgttcacc gcggccctcg
     1861 cgttgaggcc ctggagggtc tcgaggtcga ggcccttgaa caggggctcg aggaggccgg
     1921 ccacggcggg gaagcggtca agcgcgtcct tcctgatgat cggcgtgggc tcgtagatgg
     1981 gctggacctt cttcgtgtcg gcgagcacga ggaggtcgag ggcggcgagg gagccgtcgg
     2041 tcccgtaggc catcgcgaag ttgaccccgt tcgagccctg ggaggcggcc tgctcggtga
     2101 gggcggtgtt gccgcccgag aaggagagga gctggtcctt gcccaggctg aagccgtagg
     2161 ccttctggaa ggcgggcagg gcagcggggc tcgagatgaa ctcctcggag gctgccagct
     2221 tgacggcccc gccccgggcc gcgtagcggg cgaagtcctc gaggctcgcg aggccctcct
     2281 tcttggcgag gtcgcggcgg acggcgatgg cccaggtgtt gttcgcgggc gccggggtga
     2341 gccagactat cgagttcctc tgcatgtcga ggcccttgac cgtctcgtag ccctttcgct
     2401 catcctgcca gacgcttggg tctgtgccct ggaagaagaa ggccccgttg cccgtgtact
     2461 cgatgtagag gtcgagctcc ttggccacga ttgccttgcg cacgaggtcg gtcgtgccga
     2521 agcccacctt gtcggtgacg gggaccccgt tggccgcgag gagcttgacg atcatcttgc
     2581 cgaggatgga gccctcggtg tctatcttgg agccgacgac gagtggcctg gacatggact
     2641 gggaagaggc tgccccgacg atgatcaggg cggcggcgaa gaacacgagc gccttcagct
     2701 tcatgtgatg cctccttagc ccgcgggagg gggacggccc tgaatgcccg cgcgtaggga
     2761 gaaggtactg cccgcttcct tcggcggcaa taaaaggccg gcccgggccc cggcccgggg
     2821 ggcccttgcc cgtcggcggc ggaaaccggc tgcggcggac cggcggaggg gctcagggcc
     2881 ggggcgcgcc gagcctccgg gacagctcgg ccggcacgat ggcgtcgatg gtgagctggc
     2941 cctgatcggt ctcgaagcgg agatagggcc tgagctcgac ccggccgagg acgaaggcgg
     3001 ctcgggcccc ctggatctcg gcgatcctcg tgggggaggc cgcccgccac agcccgatcc
     3061 tccggccgcg gcggaggaag ctgaggccct cgccgtcaag gcagaggatg ccgccgatgc
     3121 cgtaggacca gccctcccgc tcgcggacga gcctcgcgaa acagagggcc ccctcctcct
     3181 tcgtccccgc cgcgatggcg atcctgacga gaaggaaggc gagcccggcg gagtaggcca
     3241 gggccccgac cacgaggagg gcgaggtcg
//
LOCUS       DASSQV010000501         3765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_614876, whole genome shotgun sequence.
ACCESSION   DASSQV010000501 DASSQV010000000
VERSION     DASSQV010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614876"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..3608
                     /gene="mfd"
                     /locus_tag="VFL04_08840"
     CDS             210..3608
                     /gene="mfd"
                     /locus_tag="VFL04_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET7839854.1"
                     /translation="MLDRLKGHHPLTGFLGRLESGPFPIDLAEAEGSFAAALVAMAAK
                     GRRGPILAVVPTDHEAEALENDLELMGASPMALPWWRTAAYRPASARAHAFGERASCL
                     ARLSIGEARIVVASQRAFVSPVPPRESFSSLVFELREGGSIDPQAVGERLASYGYLRV
                     PRVSLPGEFALRGEVLDICMPGEDEAFRIVFEYDRIERIVGFDPGLQSGTGRFERIIL
                     RPMKEIVWTEERVASLGRAMAQMPGCAGREGPLLEELAEKGEAKGEELWYHLAFDRTK
                     SGAPGSILDYLGAEGILVLIDHERLAAQEEAIRKEYAGLYRRALQEGPVPPPDRAILS
                     FAALEREALARELKVVRGFALKDSESEARIRMGAEAPRSFFGNVKYFKDELGTLKKAG
                     YEVLIFAETDVQAERIRSLLRDFAVEVAVAGLSAGFVVPALKLMVVQENEIFGRRKRV
                     PKSVKTARSVAIDTFVELAPGDFVVHVNYGIGRFAAIERMKVLGLERDYIKVEYAEAE
                     TVFVPIEQANLVQRYIGNEGEAPRLDRLGSKSWESRKAKVRASVEQLAERLIRIYARR
                     RTAKGYAFPPDGEWQLAFEAAFPYEETIDQLRCIEEVKSDMESQKPMDRLICGDVGYG
                     RTEIAMRACFKAASAGKQVVFLAPTTILAEQHFENLQDRIGDYPVHRAMLSRFVEKKE
                     QRKTLEGLREGKVDIVVGTHRLLQKDVDFKDLGLLVVDEEQRFGVKDKERLKEIKASV
                     DCLTLTATPIPRTLHMSLLKIRDMSVLNTPPSNRHPIETVVEEFNPDLIAEAIRREVE
                     RGGQVFFLHNRIESLQEIQTFLQGIVPEILVETAHGQMDPNELEDIMHRFIHGGFQVL
                     VSTTIIENGIDIPNVNTIIIDRADIYGISQLYQLRGRVGRSDRLAYAYLFYPDRRALN
                     ELAMKRLQIISDFTELGSGFKVAMKDLEVRGAGNLLGREQSGDIYAVGFDLYLKLLDE
                     AVQRLSDSKYEAEEEPYLELEYAGYLPESYISVPTIKMEVYKKIASIMTQADLEALLE
                     ELQDRFGPLPEEVQSLLSLAEIRVICRRLSISNLRERGGSVTVEFSKVAKVSVDRLMR
                     LLREGGGRIKLDPHRPNVLIIQTGTIGLREKSEFIRERLASLLN"
BASE COUNT          670 a         1269 c         1251 g          575 t
ORIGIN      
        1 cgggagcgcc gagggcgaag gctccggcga gggcgaaggc agcggcggcg gcagcgcgga
       61 caagggacgc tgcgggggaa gggcatcggg tcacggcctt cgttctcctt cgatctggaa
      121 caagaatgcc ggttcggcgt atactcgagg ccggctcgcc aatactagat tgtcggaatg
      181 ttatgacgaa taccttacag tcgcaagtcg tgctggacag gctcaagggc caccatccgc
      241 tgaccggctt cctcggccgc ctcgagagcg gccccttccc catagacctg gccgaggccg
      301 agggctcctt cgccgccgcc ctggtcgcca tggcggccaa gggcaggcgg ggccccatcc
      361 tcgccgtggt gcccacggac cacgaggccg aggccctcga gaacgacctc gagctcatgg
      421 gtgcctcgcc catggccctg ccctggtgga ggacggcggc ctatcgccct gcctcggccc
      481 gggcccacgc cttcggcgag cgggcctcct gcctcgccag gctctccatc ggggaggccc
      541 ggatcgtggt ggcgagccag agggccttcg tgagccctgt gcccccgcgc gagtcctttt
      601 ccagcctcgt cttcgagctc agggaggggg ggagcatcga cccccaggcc gtgggggagc
      661 gtctggcctc ctatggctac ctccgcgtgc ccagggtatc cctcccgggg gagtttgccc
      721 tccgcggcga ggtcctcgac atctgcatgc cgggggagga cgaagccttc cggatcgtgt
      781 tcgagtacga ccgcatcgag cgcatcgtcg gcttcgaccc cggcctccag tccgggacgg
      841 ggcgcttcga gcgcatcatc ctcaggccca tgaaggagat cgtctggacc gaggagaggg
      901 tggcgagcct cgggcgggcg atggctcaga tgccgggctg cgcgggcagg gagggccccc
      961 tcctcgagga gctcgcggag aagggcgagg ccaagggcga ggagctctgg taccacctgg
     1021 ccttcgaccg gaccaagtcg ggagcccccg gctcgatcct ggactacctc ggggctgagg
     1081 gcatcctcgt cctcatcgac catgagcgtc tcgctgccca ggaggaggcg atccgcaagg
     1141 agtacgccgg gctttatcgc cgcgccctcc aggagggccc ggtgcctccg cccgaccgcg
     1201 ccatcctctc cttcgccgcg ctcgagcgcg aggccctggc cagggagctg aaggtggtca
     1261 ggggcttcgc cctcaaggac agcgagagcg aggcgaggat caggatgggg gccgaggcgc
     1321 cccggtcttt cttcgggaac gtgaagtact tcaaggacga gctgggcacc ctgaagaagg
     1381 cgggctacga ggtcctcatc ttcgccgaga ccgatgtcca ggccgagagg atcaggagcc
     1441 tcctcaggga tttcgctgtc gaggtggccg tggcggggct ttcggccggt ttcgtcgtcc
     1501 ccgccctgaa gctcatggtg gtccaggaga acgagatctt cggccggcgc aaacgggtgc
     1561 cgaagtcggt gaagacggcc aggtcggtgg cgatcgacac cttcgtcgag ctcgcgcccg
     1621 gggactttgt cgtccacgtg aactacggga tcgggcgctt cgcggccatc gagaggatga
     1681 aggtactggg cctcgagcgc gactacatca aggtcgagta cgccgaggca gagacggtct
     1741 tcgtgcccat cgagcaggcc aacctcgtcc agcgctacat cggcaacgag ggcgaggcgc
     1801 ccaggctcga ccgcctcggc tcgaagtcct gggagagccg caaggccaag gtcagggcct
     1861 cggtggagca gctcgccgag cgcctcatcc gcatctacgc gcgccgcagg acggccaagg
     1921 gctacgcctt ccctcccgac ggggagtggc agcttgcctt cgaggcggcc ttcccctacg
     1981 aggagaccat cgaccagctc cgctgcatcg aggaggtgaa gtccgacatg gagagccaga
     2041 agccgatgga ccgcctcatc tgcggcgacg tcggctatgg caggaccgag atcgccatgc
     2101 gcgcctgctt caaagcggcg agcgccggaa agcaggtggt cttcctggcc cccacgacga
     2161 tcctcgccga gcagcacttc gagaacctcc aggacaggat aggcgactac ccagtgcaca
     2221 gggccatgct ctcccgcttc gtcgagaaga aggagcagag gaagaccctc gagggcctgc
     2281 gcgagggcaa ggtcgacatc gtggtcggca cccaccgcct cctccagaag gacgtggact
     2341 tcaaggacct cggcctcctc gtcgtcgacg aggagcagcg cttcggggtc aaggacaagg
     2401 agaggctcaa ggagatcaag gcgagcgtcg actgcctcac gctcaccgcg accccgatac
     2461 cgcgcaccct ccacatgtcc ttgctcaaga tcagggacat gtcggtcctc aacacccctc
     2521 cctcgaaccg ccatcccatc gagaccgtgg tcgaggagtt caaccccgac ctcatcgccg
     2581 aggccatccg cagggaggtc gagcgggggg gccaggtctt cttcctccac aacaggatcg
     2641 agagcctgca ggagatccag accttcctcc agggcatcgt ccccgagatc ctcgtcgaga
     2701 ctgcccacgg gcagatggac ccgaacgagc tcgaggacat catgcaccgc ttcatccacg
     2761 gcggcttcca ggtcctcgtc tcgacgacga tcatcgagaa cgggatcgac atacccaacg
     2821 tcaacacgat catcatcgac agggccgaca tctacggcat ctcccagctt taccagctgc
     2881 ggggccgcgt tggccgctcg gaccggctcg cctacgccta cctgttctat ccagaccgca
     2941 gggccctgaa cgagctcgcg atgaagcggc tccagatcat ctccgacttc accgagctcg
     3001 gctcgggctt caaggtggcc atgaaggacc tcgaggtccg cggggccggc aacctcctcg
     3061 gccgcgagca gtcaggcgac atctacgccg tcggcttcga cctctacctg aagctcctgg
     3121 acgaagctgt gcagcggctc tcggactcga agtacgaggc cgaggaggag ccctacctcg
     3181 agctcgagta cgcgggctac ctgcccgagt cctacatctc ggtgccgacc atcaagatgg
     3241 aggtctacaa gaagatcgcc agcatcatga cccaggccga cctcgaggcc ctgcttgagg
     3301 agctccagga ccgcttcggc cccctgcccg aggaggtcca gtccctgctc tccctggccg
     3361 agatccgggt catatgccgg cgcctctcga tcagcaacct gcgggagagg ggcggctcgg
     3421 tgacggtgga gttctccaag gtggcgaagg tctccgtcga caggctcatg cggctcctcc
     3481 gcgagggagg ggggcggatc aagctcgacc cgcacaggcc caacgtactc atcatccaga
     3541 ccgggaccat cgggctccgc gagaagagcg agttcatccg cgagcgtctg gcctccctcc
     3601 tgaactaggg cccggggccg acggaagccg gggccgggtc gggccttgct tcggtagcca
     3661 cggcggccta tcctttagga aagacagcag cccgcaacaa ggcgtctgca ccaaactggc
     3721 aagcacagcc cggtgtacgg caggcccggg accgaggtcg cacgg
//
LOCUS       DASSQV010000502         3528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1110691, whole genome shotgun sequence.
ACCESSION   DASSQV010000502 DASSQV010000000
VERSION     DASSQV010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110691"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1593
                     /locus_tag="VFL04_08845"
     CDS             292..1593
                     /locus_tag="VFL04_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HET7839855.1"
                     /translation="MPGLNVTSEIGVLRRVILHSPGPEVEAMTPKEAEADLYNDIIPL
                     AAVRAEYDTLKAFLGTVAQTFELGELLAQAIAATGNKFEFLTALGQLCPIKNRMDELM
                     AMAPAELSRAVIEGLPSLATGLPGMLSPRSFSMRPLPNTYFMRDSAAIFRDYAISGAT
                     AYDVRLIEPIITRFIFTHHPDFLAQGLLFDGPRERNRFVTIEGGDIIVIGPRVLAVGI
                     SERSSPEAVERLAVNAGKAFGEPMTVFAVLLPKERATIHLDMVFTVIDRDAALVYAPV
                     VTGPRRARVLRLDARRGGIPSITEEEGLLQGLWGAGIDLEPIACGGGDQLFQEREQWW
                     SGANSFAFAPGKIVMYSCNVHTLEALSKAGFAIKAARDFLGPAATGAEAGAGPGGAGA
                     QAVCGVDDYHRLVVGFDGIELARGGGGARCMTLPVERADLD"
     gene            1598..2149
                     /locus_tag="VFL04_08850"
     CDS             1598..2149
                     /locus_tag="VFL04_08850"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HET7839856.1"
                     /translation="MGSANGTMAAPLAQTGRRYLGIGELLVSDEELIVSTVLGSCVSI
                     LLHHPRSRLSGICHSQLAERHGEGFDCRDACPHPCGREFDSDNDYRYLSCAAARLMAD
                     FDGRGIPRSELRVSLFGGGDMVGVNVFRIGERNVKKAHKIISDWGLRIAEEDTGGRTS
                     RRLSLDMRTGKCTVALGPAPGSG"
     gene            2262..>3528
                     /locus_tag="VFL04_08855"
     CDS             2262..>3528
                     /locus_tag="VFL04_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7839857.1"
                     /translation="MNESQLFMQEDLFSAAFMSAPDIIAISGVESGVYLQVNEAFLET
                     FGLEREATIGRLTSYDIGIWVDDSFRDGLIATLRERGRVRNVEVSYRVKGGELRLFSM
                     SAGIVVLGGEDCLVSVSRDITERKLMEEGLWKSRLLLERAEEMANIGSWEVDYSRALV
                     TASPGAHRIYGLEGEQIRASDVEAAPLPEDRPRLDRARDELILKGIPYDIEFKVRRRS
                     DGRILDIHSRAVWDAEKRKLFGIIRDITDQKRAEEELKRLNLGLEERVAARTAELERA
                     QGELVNSEKMAALGQLMAGIAHELNSPLGAIGSSSGSAAACLASALAEVQSLIGEAGP
                     EMAEDCLGLLACASDPARSRSLRTSAEERAAKARAAIRLGELGLTGPSLDEAALAVAR
                     TGIEELPELLAPFFGRGLGSALRAAEGLAS"
BASE COUNT          579 a         1212 c         1192 g          545 t
ORIGIN      
        1 ctagcgtcaa gccgcggcga cactatagga atccgggttt atccctgtca ataccacgag
       61 ttatccccga cccgccccgg acgcggcccc gtgttggatg cttatgcaga gcgctcggcc
      121 gaggaagggg gttttcatgc agtcttccgg cttcgcgggc tcatgcttat gcattcgtcg
      181 cggctggccg gctcggggaa aaactgcaaa aaaagagggg tagcggtgcc cggcgtccgg
      241 ccattgctgc tgtgggccca tttcggctag agtcgcactc tggagggatc catgccaggc
      301 ctcaacgtga ccagcgaaat cggcgtcctc aggcgcgtca tactgcacag ccccgggccc
      361 gaggtcgagg ccatgacccc caaggaggcc gaggccgacc tctacaacga catcatcccc
      421 ctcgcggccg tccgcgcgga gtacgacacc ctgaaggcct tcctcggcac ggtcgcccag
      481 acctttgagc tcggcgagct cctcgcccag gccatagccg cgacgggcaa caagttcgag
      541 ttcctgaccg ccctgggcca gctctgcccc atcaagaacc ggatggacga gctgatggcc
      601 atggccccgg ccgagctcag cagggccgtc atcgagggcc tgccctccct ggccacggga
      661 ctgcccggca tgctctcccc gcgcagcttc tccatgcggc ccctgccgaa cacctatttc
      721 atgagggact cggcggcgat cttccgcgac tacgcgatct ccggcgccac ggcctacgac
      781 gtgaggctca tcgagcccat catcacccgc ttcatcttca cccaccaccc cgacttcctg
      841 gcccagggtc tgctttttga cggcccccgc gagcgcaacc gcttcgtgac gatcgagggc
      901 ggggacatca tcgtgatcgg accgcgggtc ctcgccgtcg gcatctcgga gcgctccagc
      961 cccgaggccg tcgagcgcct cgcggtcaac gcgggaaagg ccttcgggga gccgatgacg
     1021 gtgttcgccg tgctccttcc gaaggagagg gcgaccatcc atctcgacat ggtcttcacg
     1081 gtgatcgacc gcgacgccgc ccttgtgtac gcccccgtcg tgacgggacc gaggcgcgcg
     1141 agggtcctcc gcctcgacgc gcggcgcgga gggatcccct cgatcaccga ggaggagggc
     1201 ctccttcagg gcctctgggg ggccggcatc gaccttgagc cgatagcctg cggcggcggg
     1261 gaccagctct tccaggaaag ggagcagtgg tggtcggggg ccaactcctt cgccttcgcc
     1321 cccgggaaga tcgtcatgta cagctgcaac gtccacaccc tcgaggccct ctccaaggct
     1381 ggattcgcca tcaaggcggc ccgcgacttc cttgggcccg cagcgaccgg ggccgaggcg
     1441 ggagcggggc cagggggggc cggggcccag gccgtctgcg gcgtcgacga ctaccaccgc
     1501 ctggtcgtgg gcttcgacgg catcgagctc gcgcgcggag ggggaggggc ccgctgcatg
     1561 accctgcccg tcgagcgcgc ggatttggac taggatgatg ggaagcgcga acgggaccat
     1621 ggcagcgcct ctggcccaga cggggcgccg ctatcttggc atcggggagc tcctcgtctc
     1681 cgatgaggag ctcatcgttt ccaccgtcct gggctcctgc gtctcgatcc tcctgcacca
     1741 cccgcggagc cggctctcgg gcatctgcca ctcccagctg gccgagaggc atggtgaggg
     1801 cttcgactgc cgcgacgcct gcccccatcc ctgcggcagg gagttcgaca gcgacaacga
     1861 ctaccgctat ctctcatgcg cggcggcacg cctcatggcc gatttcgacg gtcggggcat
     1921 accgcgctcg gaactccgcg tcagcctctt cggcggcggg gacatggtcg gggtgaacgt
     1981 cttcaggatc ggcgagcgca acgtgaagaa ggcccacaag atcatctcgg actggggcct
     2041 gaggatcgcc gaggaggaca cgggcgggcg cacatcgcgg cgactcagcc tcgacatgag
     2101 aacggggaaa tgcaccgttg ccctggggcc agccccgggc tcgggctgag gccccgaccg
     2161 cccctacttc gcttgacttc cgccggccgc gcgctagagt tctggttgcg ccgctgttga
     2221 ggcccaccgt aacccggcgc ggggagattt cgggcaccca catgaacgaa agccagctct
     2281 tcatgcagga ggatctcttc tccgccgcct tcatgagcgc gcccgacatc atcgccatca
     2341 gcggcgtcga gtcgggcgtc tacctccagg tcaacgaggc cttcctcgag acctttggcc
     2401 tcgagcgcga ggcgaccatt ggacgcctga cctcgtacga catcggcatc tgggttgacg
     2461 actccttccg cgacgggctc atcgccaccc tcagggagcg gggccgggtg cggaacgtcg
     2521 aggtgagcta ccgcgtcaag ggcggagagc tgcggctctt cagcatgtcg gccggcatcg
     2581 tggtcctcgg gggagaggac tgcctcgtca gcgtgagccg ggacatcacc gagcgcaagc
     2641 tgatggagga ggggctctgg aagagcaggc tcctgcttga gcgcgccgag gagatggcga
     2701 acatcggcag ctgggaggtt gactactcga gggccctggt cacggcctcc ccgggggcgc
     2761 accggatcta cggcctcgag ggggagcaga tcagggcctc agacgtggag gccgctcccc
     2821 tgcccgagga caggccgagg ctagacaggg cgagggacga gctcatcctg aagggcatcc
     2881 cctatgacat cgagttcaag gtgaggagga ggtcggacgg ccgaatcctc gacatccatt
     2941 cccgcgccgt ctgggacgcc gagaagagga agctcttcgg gatcatccgc gacatcaccg
     3001 accagaagcg ggcagaggag gagctcaagc ggctcaacct cggcctcgag gagagggtcg
     3061 ccgcccgcac ggccgagctc gagcgggccc aaggcgagct agtcaactcc gagaagatgg
     3121 ccgccctcgg ccagctgatg gccgggatag cgcacgagct gaacagcccc ctcggggcga
     3181 taggctcgtc atcgggctcg gcggcggcct gcctggcctc ggccctcgca gaggtccaaa
     3241 gcctcatcgg cgaggccggc ccggaaatgg ccgaggactg cctcggcctc ctggcctgcg
     3301 ccagcgatcc ggcgcggagc cggtccctcc gcacgagcgc cgaagagcga gcagccaagg
     3361 caagggcggc gatccgcctg ggggagctcg gccttaccgg ccccagtctg gatgaggccg
     3421 ccctcgccgt agcgcgcacc gggatcgagg agctgcccga gctccttgct ccctttttcg
     3481 gcaggggcct tggttcggcg ctcagggccg ccgagggcct ggcctccc
//
LOCUS       DASSQV010000503         5203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_865097, whole genome shotgun sequence.
ACCESSION   DASSQV010000503 DASSQV010000000
VERSION     DASSQV010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865097"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2093
                     /locus_tag="VFL04_08860"
     CDS             <1..2093
                     /locus_tag="VFL04_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HET7839858.1"
                     /translation="SLDDGRPITGLRGMAQAADGRIYAGTDGLGLLSVDPRTGSIREL
                     PGMPADRASKKVYAVLVARDGTLWAGTDGAGLLARSPDGSVRSYRHETSRPGSLASDV
                     VWSLFEDRDGSLWVGTEGGGLDLLPPGASAFVHHRPEAPRGGSISGSSIRAVFRDSRG
                     RLWAGAWDGGLSRLDPGSAAFTSFGPDSRVPSSLGDASVNCIFEDSSGSIWVGTGGSG
                     IARLDEASGRFAHYSTEEGLAGATVYGILQDRAGALWISTATGLSRYLPNTGGFFNFG
                     SEDGLASALLYQNSFLKSASGELWLGGRDGLTRFDPGRIPVRGSPPEVAISELDAGGR
                     RLSPGSAATGGAVELPWDNPGLSLRIAVLDYVAPDRNRYAMSLSGRQDAWTQLGTSNL
                     GSIAPLPPGDYLLRVKGSNGNGVWNEEGRTLRLLVHPPVWGTWWFRLIAASLVAAAAA
                     GAIAVRIGSLHRRNALLVNFARHIEAAREEERTNASREVHDEIGQHLAVLNLQAYWLG
                     THPEAEPESRKARLVDMTSSIAAAMSAVKAVSTRLRPVALDSLSLGETIRWYLKDFER
                     RSGIACRCEALPLPQELSDGVATALFRVLQESLSNVARHAGARSVEVRLHVEGGFLVL
                     EVADDGSGIAEASIHASDSFGIIGMRERCAALGGSLRISGQAGRGSVVVARVPAAGPA
                     PGAASGASAAKGDR"
     gene            2096..2734
                     /locus_tag="VFL04_08865"
     CDS             2096..2734
                     /locus_tag="VFL04_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7839859.1"
                     /translation="MLRILVADDHFLIRKGLRQLIEESLPVSRLDEAEDGVSTMAQAR
                     ANRYDVVILDISMPGKDGLDLIRDIKEADPEAKVLVLSISPEEQYALRAFKLGASGCL
                     NKSGDPGELVDAIRTVVGGRRYLSPKTQELLLEDSVGAGDRPPHELLSDREYQVFRLL
                     ASGKPIGEIAALLSLSVKTVSTYRARVLGKMGMENNAQLTHYAFKNGLVGGP"
     gene            2826..3914
                     /locus_tag="VFL04_08870"
     CDS             2826..3914
                     /locus_tag="VFL04_08870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7839860.1"
                     /translation="MPPSSGPELRIHPPFLGEEREELAAFLRSAGLEPEPGMAYTVAC
                     LEGGRIAAVGSLSGRVIRGLAVSPERQGEGLAATVVSALEAEAGARGLVRLLVYTSPD
                     KQAIFSSLGYRLIAESGEDAVLLEKGGGIESWCAELSRALPRPPTGFEAPPTGVEAAP
                     TAALVMNCNPFTLGHRHLVEAAARRASRVALFVVSEEASSFPYEVRLRLVREGTADLQ
                     KVTVIPGTDYLISRATFPTYFLKDRAGAAAAIHARLDLDLFARHVAPAVGASLRLVAE
                     EPYSEVTRLYNRIMQEVLPPRGIAVEVIPRLEAGGQAVSASTVRRLIREGSLGDIRGL
                     VPECTWAYLNSPEAKDVLERVRATEGRH"
     gene            3934..4221
                     /gene="citD"
                     /locus_tag="VFL04_08875"
     CDS             3934..4221
                     /gene="citD"
                     /locus_tag="VFL04_08875"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HET7839861.1"
                     /translation="MEIIKTGIAGTLESCDASVTVERAAELELLVESADRRFAKSVRQ
                     AALAALAELGVKTARVTVREKSALDCTVRARVLTACLRAAGLESAPWEALK"
     gene            4218..5111
                     /locus_tag="VFL04_08880"
     CDS             4218..5111
                     /locus_tag="VFL04_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7839862.1"
                     /translation="MTNRLRRTMLFVPGNNPALVKDAQIYGSDSIIFDLEDSVAPSEK
                     DSARLLVWSALRSIDYGKVEKVVRINALDSPWFREDLEAMVRAGIDLVRLPKTEGPDD
                     IRKLEELVADLERSAGRPEGSVRLMAALEGPLAVLNAYEIARSSRRLVAIALSAEDFV
                     TSMRTTRSAEGVELAEARGRIVLAARAAGVMAIDTVFTDVADTEGFLREVRLAKQMGF
                     DGKSVITPAQIPLVHGVFEPGPEELRRARRVVAAAAEAERRGLGVISLDGKMVNKPIV
                     DRALGLIDLARASGQRVEEVE"
BASE COUNT          766 a         1985 c         1709 g          743 t
ORIGIN      
        1 tgagcctcga cgacggccga cccatcacgg gcctccgcgg catggcccag gccgccgacg
       61 gccggatcta cgcgggcacc gacggcctcg gcctcctctc cgtagacccg cggacgggct
      121 cgatccgcga gcttccgggc atgcccgcgg accgggcctc gaagaaggtc tacgccgtcc
      181 tcgtggcgag ggacggcacc ctctgggccg gcaccgacgg ggccggcctc ctcgcccgct
      241 cccccgatgg ctcggtcagg agctaccgcc acgagacctc gaggccggga agcctcgcga
      301 gcgacgtcgt ctggagcctt ttcgaggaca gggacggctc gctctgggtc ggcaccgagg
      361 gcgggggcct cgacctcctg cccccggggg cctcggcctt cgtccaccac aggcccgagg
      421 ccccgcgcgg cggctcgatc tcgggctcct ccatccgcgc cgtcttcagg gactcgcggg
      481 gcaggctatg ggcaggggcc tgggacgggg gcctctcgag gctcgacccc ggctccgccg
      541 ccttcacctc cttcgggccc gactcccgcg tcccttcctc cctcggcgac gcgagcgtca
      601 actgcatctt cgaggacagc tcgggctcca tctgggtggg cacgggggga tcgggcatcg
      661 cgcgcctcga cgaggcctcg gggcgcttcg cccactattc caccgaggag ggccttgccg
      721 gggcgaccgt ctacggcatc ctccaggaca gggcaggagc cctctggatc tcgaccgcca
      781 cgggcctctc gcgctacctc cccaacacgg gcggtttctt caacttcggc tccgaggacg
      841 gcctcgcctc ggccctcctt taccagaact ccttcctgaa gagcgcctcg ggcgagctct
      901 ggctcggcgg cagggatggc cttacccgct ttgaccccgg caggatcccg gtgcggggga
      961 gcccccccga ggtcgcgatc agcgagctgg acgcgggcgg ccggcgtctc tcccccggtt
     1021 cggcggccac gggcggggcg gtcgagctcc cctgggacaa ccccggcctc agtctgcgga
     1081 tcgccgtcct cgactacgtg gcccccgacc gcaaccgcta cgccatgagc ctctcgggcc
     1141 gacaggacgc ctggacccag ctcggcacga gcaacctcgg gagcatcgcc cccctgcctc
     1201 ccggggacta cctcctcagg gtcaagggct cgaacggcaa cggggtctgg aacgaggagg
     1261 gcaggaccct caggctcctc gtccacccgc ccgtctgggg gacctggtgg ttccgcctca
     1321 tcgcggcgag cctcgtggcc gccgcggcgg caggggccat cgccgtcagg atcggctccc
     1381 tccatcgtcg caacgccctc ctcgtcaact tcgcccgcca catcgaggct gcgcgcgagg
     1441 aggagcgcac gaatgcctcg cgcgaggtcc acgacgagat cggccagcac ctggccgtcc
     1501 tcaacctcca ggcctactgg ctcggtaccc accccgaggc cgagccggag agccggaagg
     1561 ccagactcgt cgacatgaca tcctcaatcg ccgccgcgat gtcggcggtc aaggccgtct
     1621 cgacccggct caggcccgtg gcccttgact ccctctccct gggcgagacc atccgctggt
     1681 acctcaagga cttcgagcgc cgctcgggca tcgcctgccg ctgcgaggcc cttcccctgc
     1741 cccaggagct ctcggacggg gtggcgaccg ccctgttccg cgtcctccag gagtccctga
     1801 gcaacgtcgc ccgccacgcg ggggcccgct cggtcgaggt caggctccac gtcgaggggg
     1861 gcttcctcgt cctcgaggtc gccgacgacg gctcgggcat agccgaggcc agtatccacg
     1921 cgagcgactc cttcggcatc ataggcatga gggagcgctg cgccgccctc ggcggctcgc
     1981 tccgcatctc gggccaggcg gggaggggga gcgtcgtcgt cgcgcgcgtg cccgccgccg
     2041 gcccggcccc gggcgcggcc tccggggcca gcgccgcgaa gggagacaga tagccatgct
     2101 gcggatcctc gtcgccgacg accatttcct catccggaag ggcctgcgcc agctcatcga
     2161 ggagagcctg cccgtctcga ggctcgacga ggccgaggac ggggtgagca cgatggccca
     2221 ggccagggcc aaccgctacg acgtcgtcat cctcgacatc tccatgcccg gcaaggacgg
     2281 cctcgacctc atcagggaca tcaaggaggc cgaccccgag gccaaggtcc tcgtcctctc
     2341 gatctccccc gaggagcagt acgccctcag ggccttcaag ctcggggcct cgggctgcct
     2401 caacaagtcg ggcgaccccg gcgagctcgt cgacgcgatc aggaccgtcg tgggggggcg
     2461 gcgctacctc agccccaaga cccaggagct cctcctcgag gacagcgtgg gcgctggcga
     2521 ccggcccccc cacgagctcc tctccgaccg cgagtaccag gtcttccgcc tcctcgcctc
     2581 gggcaaaccc atcggggaga tcgcggccct cctctcgctc agcgtgaaga ccgtgagcac
     2641 ctaccgcgcc cgggtcctcg ggaagatggg catggagaac aacgcccagc tcacccacta
     2701 cgccttcaag aacggcctcg tcggcgggcc ctagtcccct gcccgacccc tgtaggactg
     2761 gcacctacag gggaaactca ggccatccta catacccggg gcgcggcctg gcctataatc
     2821 cggccatgcc cccctcctcc ggacccgagc tccgcatcca cccccccttc ctcggggagg
     2881 agcgcgagga gctcgccgct ttcctccgct cggcggggct cgagcccgag cctggcatgg
     2941 cctacaccgt cgcctgcctc gagggcggca ggatcgccgc cgtgggctcc ctctccggcc
     3001 gcgtgatccg cggcctcgcc gtctcgcccg aacgccaggg cgagggtctc gcggccaccg
     3061 tcgtgtccgc cctcgaggcc gaggccggcg cccgcggcct tgtgcgcctc ctcgtctata
     3121 cctcaccgga caagcaggcc atcttctcct cccttggtta ccggctcatc gccgagtcgg
     3181 gtgaggacgc cgtcctcctc gagaagggcg gaggcatcga gtcctggtgc gccgagctct
     3241 cccgggccct cccgcggcca ccaacaggct tcgaggcacc accaacaggc gtcgaggcag
     3301 cccccacggc cgccctcgtc atgaactgca accccttcac cctgggccac cgccaccttg
     3361 tcgaggccgc ggcccgccgc gcctcccgcg tcgccctctt tgtcgtgtcc gaggaggcct
     3421 cgagcttccc ctacgaggtc aggctccgcc tcgtccgcga gggcacggcc gaccttcaaa
     3481 aggtcaccgt catacccggg accgactacc tcatctcccg ggcgaccttc cccacctatt
     3541 tcctcaagga cagggcgggc gccgctgccg ccatccacgc ccggctcgac ctcgacctgt
     3601 tcgcgcgcca tgtggcgccc gccgtcggag cgagccttcg cctcgtggcc gaggagccct
     3661 acagcgaggt cacgaggctc tacaacagga tcatgcagga agtgctgccc cccaggggga
     3721 tagccgtcga ggtgataccc cggctcgagg cgggcggcca ggccgtgagc gcctcgaccg
     3781 tgcgccgcct catcagggag ggcagcttgg gcgacatcag aggcctcgtg ccggagtgca
     3841 cctgggccta tctgaactcc cccgaggcca aggacgtgct cgagcgcgtc agggccacag
     3901 agggcaggca ttaggcaagg agataagggg agcatggaga tcatcaagac cggaatcgcc
     3961 gggaccctcg agtcctgcga cgcctccgtc accgtcgaga gggccgccga gctcgagctc
     4021 ctcgttgaga gcgccgacag gcgcttcgcg aagagcgtgc gccaggccgc cctggccgcc
     4081 ctcgccgagc tcggggtcaa gacggcccgc gtcaccgtgc gcgagaagag cgcgcttgac
     4141 tgcaccgtca gggcgagggt cctgacagcc tgcctccgcg ccgccgggct ggagagcgcg
     4201 ccatgggagg ccctgaagtg accaacaggc ttcgtcgcac aatgctcttc gtccccggca
     4261 acaaccccgc cctcgtcaag gatgcccaga tctacggctc ggactcgatc atcttcgacc
     4321 tcgaggactc ggtcgccccc tccgagaagg actcggcccg cctcctcgtg tggtcggccc
     4381 tccgcagcat cgattacggg aaggtcgaga aggtcgtcag gatcaacgcc ctcgacagcc
     4441 cctggttccg cgaggacctc gaggccatgg tccgcgccgg aatcgacctg gtccgcctcc
     4501 ccaagaccga gggccccgat gacatcagga agctcgagga gctcgtggcc gacctcgaga
     4561 ggtccgccgg caggcccgag ggctcggtgc ggctcatggc cgccctcgag ggtcccctgg
     4621 ccgtgctcaa cgcctacgag atcgcccgct caagccggcg cctcgtggcg atcgccctct
     4681 cggccgagga cttcgtgacg agcatgagga ccacccgctc ggccgagggc gtcgagctcg
     4741 ccgaggcgag gggtcgcatc gtcctcgcgg ccagggccgc aggcgtcatg gccatcgaca
     4801 cagtcttcac cgacgtcgcc gacaccgagg gcttcctccg cgaggtgagg ctggccaagc
     4861 aaatgggctt cgacggcaaa tccgtcatca cccccgccca gatccccctt gtccacggcg
     4921 tcttcgagcc ggggcctgag gagctcaggc gggcccgccg cgtggtggcc gccgccgccg
     4981 aggccgagcg gagggggctc ggcgtcatct ccctcgacgg gaagatggtc aacaagccca
     5041 tcgtggacag ggccctgggc ctcatcgacc tggccagggc ctcgggacag cgcgtggagg
     5101 aggtggaatg aggggcaagt ctgagggagg gcgggagatc cccgcagtca tccccggttt
     5161 cggcaaggtc ctgcccttcg cgggggcctg gtccaattcg gcc
//
LOCUS       DASSQV010000504         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1861186, whole genome shotgun sequence.
ACCESSION   DASSQV010000504 DASSQV010000000
VERSION     DASSQV010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861186"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..2667
                     /locus_tag="VFL04_08885"
     CDS             259..2667
                     /locus_tag="VFL04_08885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET7839863.1"
                     /translation="MSDYLDPNNEELLKDFFSEANGQVETLEQNVLVLENDPSNRDAI
                     DEIFRAAHTLKGGSGTVEMTELAEFTHLVEDVLDGLRSGVAEVSESLIDALLGSIDVI
                     KAMLRAREAKTAYRGDTTAVKASLASFLPAGAVSAKHLAPKPAAKAEPKRPGPASSAT
                     DLSEYEMLELRETAGADRRVFRLTLNFNPDNIMKTVSAIHAFAALRDTGTVLKTVPDF
                     EKLYEDAFYPTVTYYLAADKDPEELRRKAMIPDVVLEVELEELLVETGPKAAAPVAKA
                     AAPAAKAVATAQAPLAPAAKPPRQAPAKPEHPAPAEAEVPEEAVLAAPAQEDEHKDVE
                     SRRNSQAGSILRVDSRRIDNLLNLVSETVINKATFNQISAQFGELMGSLQTAQSKFRD
                     GLKELFDDLPEQLAGLQAGKTPKQIKKEITEKYGSIYQAFDGVEASLKTDVAKFRSTA
                     QNLGRITGELQEGVMRIRMVPISQIFSRFPRLVRDLTKSLNKKVDLVIEGEETELDKS
                     VIEDLLDPLMHSVRNALDHGIETPEERRAAGKPEEGTVLLKASNEGNMIIIEIADDGK
                     GIDVEAVRTKAVERGLIHPSKVLTDIEAFNLIFDAGFSTAKSVTNISGRGVGLDVVKR
                     QIEKLNGSVTVSSQRGKGTRFTIKLPLTLAIIQGLLVRVGREIYSIPITSVIESHRIK
                     PSDIKMIDNYEVFNIRNDVVALLRLNRVFRIETAEQRDYHFVVIVGTAEKRMGLMVDS
                     LIGEEDVVIKPLRDQFTQSPGIAGASILGDGSVCLIIDVSQLLELGLKRELEERRRLE
                     ATIR"
     gene            2727..>3177
                     /locus_tag="VFL04_08890"
     CDS             2727..>3177
                     /locus_tag="VFL04_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7839864.1"
                     /translation="MAEAVKRAGKVDFKMVTFSLAGKEYGIDIMSVKEIAKAGRFTYV
                     PNAATYVRGVYNLRGEIISVIDLRIFFHLPAERKAEDALESLLILRIEEHVFGVIVDA
                     IDKVVGISSSAIQPPHPIFGDINVKYIKGIVENSGKLYIILDVEKIFA"
BASE COUNT          659 a         1080 c          965 g          473 t
ORIGIN      
        1 gaaggcgcgg cgagcatgag gacgagatag gcgatgacaa tggcggctgc tttcttgggc
       61 ataatgtcga ctcccggcct gaagggccgt cattgtaatt ctagcgccga tcggattgga
      121 aagcacggcg cgatactatt atcggtcgga atatcggcca atggagccga aaggaccgtc
      181 ccttctcgcc cgaaaagctt tcgagtcatg acaaaccccg ttgacggatc gtcccatcta
      241 tataatgctg gaagaacgat gagcgactac ctcgatccga acaacgagga actcctgaag
      301 gatttcttct cggaagccaa cggccaagtt gagactctcg agcagaacgt cctggtcctc
      361 gagaacgatc cgtcgaacag ggacgcaatt gacgagatct tccgggcagc ccacaccctc
      421 aagggcgggt cggggaccgt ggagatgacc gagctggcgg aattcaccca cctcgtcgag
      481 gacgtgctcg acggcctccg ttcgggagtc gccgaggtca gcgaatccct gatcgacgcc
      541 ctcctcgggt cgatcgacgt catcaaggca atgctgcggg cccgcgaggc caagactgcc
      601 taccgcggcg acacgacggc ggtgaaggcc tccctggcct ccttccttcc ggccggcgcc
      661 gtctcggcca agcacctcgc gcccaaaccg gccgccaagg ccgaaccgaa gcgtcccggc
      721 ccggcctcct cggccaccga cctgagcgaa tacgagatgc tcgagctgcg cgagaccgcc
      781 ggagccgacc ggcgggtctt ccgcctgacc ctcaacttca accctgacaa catcatgaag
      841 accgtgtcgg cgatccacgc cttcgccgcc ctccgcgaca cgggcacggt gctcaagacg
      901 gtcccggact tcgagaagct ctacgaggac gccttctacc ccacggtgac ctactacctc
      961 gccgccgata aggatcccga ggagctccgc aggaaggcga tgatccccga cgtcgtcctc
     1021 gaggtcgagc tcgaggagct cctcgtcgag accggcccca aggccgctgc ccccgtggcg
     1081 aaggccgccg cccccgctgc gaaggctgtc gccacggccc aggctcctct ggccccggcc
     1141 gcgaagccgc cgaggcaggc ccccgcgaag cccgagcacc cggcccccgc cgaggccgaa
     1201 gtcccggaag aggcggtcct cgcggcccct gcccaggagg atgagcacaa ggacgtcgag
     1261 agccgcagga acagccaggc cggctcgatc ctcagggtcg acagccggcg gatcgacaac
     1321 ctcctcaacc tcgtgtccga gaccgtcatc aacaaggcta ccttcaacca gatctcggcc
     1381 cagttcggcg agctcatggg cagcctccag actgcccagt ccaaattccg cgatggcctc
     1441 aaggagctct tcgacgacct gcccgagcag ctcgccggcc tccaggccgg caagacgccg
     1501 aagcagatca agaaggagat caccgagaag tacggttcga tctaccaggc cttcgacggc
     1561 gtcgaggcct cgctcaagac cgacgtggcc aagttccgct cgacggccca gaacctcggc
     1621 cgcataacgg gcgaactcca ggaaggcgtc atgcgcatcc gcatggtgcc gatcagccag
     1681 atcttcagcc gcttcccacg gctcgtgcgc gacctcacca agtccctgaa caagaaggtg
     1741 gacctcgtca tcgaaggcga ggagaccgag ctcgacaagt cggtcatcga ggacctcctc
     1801 gaccccctca tgcactcggt gcgcaacgcc ctcgaccatg gcatcgagac cccggaggag
     1861 cgccgcgcgg ccggcaagcc cgaggagggc accgtcctcc tcaaggcctc caacgaaggc
     1921 aacatgatca tcatcgagat cgccgacgac gggaagggga tcgacgtcga ggcggtccgc
     1981 accaaggccg tggagcgtgg gctgatccac cccagcaagg tgctcaccga catcgaggcc
     2041 ttcaacctca tcttcgacgc gggcttctcc acggcgaagt cggtgaccaa catctcgggc
     2101 cgcggggtcg gcctcgacgt ggtgaagcgg cagatcgaga agctcaacgg ctcggtcacg
     2161 gtgtccagcc agcgcggcaa gggcacccgc ttcacgataa agctccccct cacgctcgcg
     2221 atcatccagg gcctcctggt gcgggtgggc agggagatct actcgatccc catcacgagc
     2281 gtcatcgaga gccaccgcat aaagccaagc gacatcaaga tgatcgacaa ctacgaggtc
     2341 ttcaacatcc gcaacgacgt cgtggccctc cttcggctga accgcgtgtt ccggatcgag
     2401 acggccgagc agcgcgacta ccacttcgtg gtcatcgtgg gcacggcgga gaagcggatg
     2461 gggctcatgg tcgactccct catcggggag gaggacgtgg tcatcaagcc cctgcgcgac
     2521 cagttcacgc agtcgccggg catcgccggg gcctcgatcc ttggagacgg ttcggtctgt
     2581 ctcatcatcg acgtgagcca gctgctcgag ctcggcctca agcgagagct cgaggaacgg
     2641 cgccggctcg aggccaccat ccgctagaac ggcgaggaga gagcatggcg gcgcagacag
     2701 cagaacagca gcagagcaac gcggaaatgg ccgaggccgt gaagcgggcg ggcaaggtcg
     2761 acttcaagat ggtgaccttc tcgctcgcgg gcaaggaata cggcatcgac atcatgagcg
     2821 tgaaggagat cgccaaggcg ggtcgcttca cctacgtccc caacgccgcg acctacgtca
     2881 ggggcgtcta caacctcagg ggcgagatca tctcggtcat cgacctgcgg atcttcttcc
     2941 acctgccggc cgagcggaag gccgaggacg cgctcgagag cctcctgatc ctcaggatcg
     3001 aggagcacgt gttcggcgtc atcgtcgacg cgatcgacaa ggtcgtgggc atctcctcgt
     3061 cggccatcca gcccccccac ccgatcttcg gcgacatcaa cgtcaagtac atcaagggca
     3121 tcgtcgagaa ctcgggcaag ctctacatca tcctggacgt cgagaagatc ttcgcgc
//
LOCUS       DASSQV010000505         5185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_367478, whole genome shotgun sequence.
ACCESSION   DASSQV010000505 DASSQV010000000
VERSION     DASSQV010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367478"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VFL04_08895"
     CDS             complement(<1..409)
                     /locus_tag="VFL04_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HET7839865.1"
                     /translation="MTATGRAGFNLEKYGSLLALGVLFVLAAIIGWPYFLQIENLVNV
                     MRQSSYTGIIALGMTFVIISGGIDLSVGAIAAFVASCGVLFMNYTLAHNGGNESVAIL
                     AGIGVMLALGFVCGALNGFLITKGKIVPFIVTLG"
     gene            complement(406..1917)
                     /locus_tag="VFL04_08900"
     CDS             complement(406..1917)
                     /locus_tag="VFL04_08900"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET7839866.1"
                     /translation="MGEVILRAEGINKDFGGVLVLKDVSLDVRRGEILGIIGENGAGK
                     STLMKILSGIYQPTSGRVLVEGKSVRIDTAIDARRLGISLVPQEFNLIRDLPVYNNVF
                     LGSELTTPLGLLDKAAMKARTRELLAELNVTIDPEARIEGMSAAEKQMVEICKALAFA
                     AGVLIMDEPTTMLTTYEIEILFSLVRRLRDRGMTVIYISHKLKEVKALCDRVAVLRDG
                     VLVHTSPVAEISVLEMAQRMVGRELGSIFPPKGEGSAEVVFEARAVTVPGVVEDISFE
                     LHRGEILGLAGLVGAGRTEVAETILGLRKKSAGEFLKRGKVLRLEEPADAVAAGISYL
                     SEDRQGAGIVTGFTVTENVTLVSLANYCRSLLRLIDKGEERRKVQGDVDGFRIKTESI
                     DKRLENLSGGNQQKVSLAKSIDTGPEILIVDEPTRGVDVSAKHEIYLFINDLAKKGIS
                     CLFISSELEEVIGMCNRVLVMRDGKIAATLEGRDINEEEIMFNATGAHEEYAS"
     gene            complement(1926..2915)
                     /locus_tag="VFL04_08905"
     CDS             complement(1926..2915)
                     /locus_tag="VFL04_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET7839867.1"
                     /translation="MARPVTIISGQWADLGFETLCEKVSAWGYEGIEIANLGDHLDVR
                     RAATDPRYVEERKAILKRHGLGVWAISAHLTGQCVGDPWDPRLDGFAPPEVKGKPEAI
                     VAWAIEDMKHAARAAKALGASVVTCFMGSPIWRYWYSFPQTTEAMIEEAYAEIVRRWT
                     PILDVFDECGVRFALEVHPTEIAFDYYTAKRLLEAFGHRRTLGFNFDPSHLLWQGVRP
                     ELFIRDFADRIYHVHMKDVAVRSDGRSGILGSHLSFGDLRRGWNFVSLGHGDVDFDAI
                     IRELNAAGYAGPLSVEWEDTGMDREYGAREAIEYVRRHDFQASAQAFDAAMRK"
     gene            complement(2926..3921)
                     /locus_tag="VFL04_08910"
     CDS             complement(2926..3921)
                     /locus_tag="VFL04_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7839868.1"
                     /translation="MKSEALWYLGERRIEYRTMDLEDPGPNEVLVQMEACGICGWDLL
                     AYSGRFAKFHDYPFTAGHEGVGRIVRKGERVEGLELGQRVACHELPIGSRGGGLMARH
                     ALRATDKVSAIPEGSTIPLTRWVVEPVACVVNGLVYAGIKPGDSVALVGAGYMGLLML
                     QALRRTMAGRVSVFEPDARRLELARSFAAGMGGWDFQAPAVEPAPGAEPGKGRERVYD
                     VVIETAGSAASLELAFALAKPYAIIENFAWHHHSQEFDLDQWHTKGWRILNIQPQMNP
                     AFDALFPAAIKLMAAGAISNEGLVTHVASFAKAEEAYAAGLSRTGGYIKGVIEFE"
     gene            4058..>5185
                     /locus_tag="VFL04_08915"
     CDS             4058..>5185
                     /locus_tag="VFL04_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET7839869.1"
                     /translation="MLHRMGSLAEADGSPANGSTTAGSIFKIIRDHGPLYGNDIVRRT
                     GLAKSTVSAYLDRLVSVGLVREEIPEGGKRRKLKVAESAGYVVGVALGQTHLNVALCD
                     LEAEIIETVGGEVDLVRESPETVLGKVVEYTHELERRAGLGPDSLFGIGLGLPSPVDY
                     ARGMPVTPPVMPGWDRFPVASFLANEFTCPVFVDNDVNVMALAERDKGAAAELAADSG
                     CFLLVKAGTGIGAGIVINGEIYRGAKGAAGDIGHIGIDGDDTLCRCGNVGCLEAVAGG
                     WALASRAELAARSQRSPFLAEAVAEGRAISPELLARGAASGDDECLRLIISAGKYIGD
                     VLAKLVNFFNPSFLVVNGRLANLGERFIASIRESVYRRSTPL"
BASE COUNT          795 a         1698 c         1824 g          868 t
ORIGIN      
        1 tgcccagggt gacgatgaag gggacgatct tgcccttggt gatgaggaag ccgttcaggg
       61 ccccgcagac gaagccgagg gccagcatga cgccgatgcc ggcgaggatg gcgacgctct
      121 cgttgccgcc gttgtgcgcg agcgtgtagt tcatgaacag gacgccgcag gaggccacga
      181 aggcggctat cgcgcccacc gagaggtcga tgccccccga gatgatgacg aaggtcatgc
      241 ccagggcgat gatgccggtg taggaggact ggcgcatgac gttgacgagg ttctcgatct
      301 ggaggaagta gggccagccg atgatcgcgg cgaggacgaa gaggacgccc agggcgagga
      361 gggacccgta cttctcgagg ttgaagcctg cgcggccggt tgccgtcatg acgcgtactc
      421 ctcgtgcgct ccggtggcgt tgaacatgat ttcctcttcg ttgatgtcgc ggccctcgag
      481 ggtcgcggcg attttcccgt cccgcatgac gaggacccgg ttgcacatgc cgatgacctc
      541 ctcgagctcc gaggagatga acaggcagga gatgcccttc ttggcgaggt cgtttatgaa
      601 caggtagatc tcgtgcttgg ccgagacgtc gaccccgcgc gtgggctcgt cgacgatgag
      661 gatctcgggc ccggtgtcga tggacttggc gagcgagacc ttctgctggt tcccgcccga
      721 gaggttctcg agccttttgt cgatgctctc ggtcttgatc ctgaagccgt cgacatcgcc
      781 ctggaccttc cgcctctcct ctcccttgtc gatgagccgc aggagggagc ggcagtagtt
      841 ggccagggac acgagggtga cgttctcggt gacggtgaag cccgtgacga tgcccgcccc
      901 ctgcctgtcc tcggagaggt aggagatgcc ggcggccacg gcgtcggcgg gctcctcgag
      961 cctgagcacc ttgccccgct tcaggaactc gccggcgctc ttcttgcgga ggccgaggat
     1021 ggtctcggcc acctcggtgc ggcccgcgcc cacgaggccc gcgaggccga ggatctcgcc
     1081 ccggtgcagc tcgaagctga tgtcctcgac gacgccgggc acggtgacag cgcgggcctc
     1141 gaagaccacc tcggccgagc cctcgccctt gggggggaag atcgagccga gctcgcggcc
     1201 caccatgcgc tgggccatct cgaggacgct gatctcggcg accggcgagg tgtggacgag
     1261 gaccccgtcg cggaggacgg ccacccggtc gcagagggcc ttcacctcct tgagcttgtg
     1321 cgagatgtag atgacggtca tgccgcggtc gcggagccgg cgcacgaggg agaagaggat
     1381 ctcgatctcg taggtcgtga gcatggtcgt gggctcgtcc atgatgagga cgcccgcggc
     1441 gaaggcgagg gccttgcaga tctccaccat ctgcttctcg gcggcgctca tgccctcgat
     1501 cctggcctcg gggtcgatgg tcacgttgag ctcggcgagg agctcgcggg tcctcgcctt
     1561 catggccgcc ttgtcgagga gcccgagggg ggttgtgagc tcggagccga ggaagacgtt
     1621 gttgtagacc gggaggtcgc ggatgaggtt gaactcctgg ggcacgaggc ttatgccgag
     1681 ccgcctcgcg tcgatcgctg tgtcgatgcg gacgctcttg ccctcgacga ggaccctgcc
     1741 cgatgtgggc tggtagatgc cgctgaggat cttcatgagg gtgctcttgc cggccccgtt
     1801 ctcgccgatg atgccgagga tctcgccgcg gcgcacgtcg aggctcacgt ccttgagcac
     1861 gaggaccccg ccgaagtcct tgttgatgcc ctcggcccga aggatgacct cgcccatcgc
     1921 gccgctcatt tcctcatcgc cgcgtcgaag gcctgggccg aggcctggaa gtcgtgcctg
     1981 cgcacgtact cgatggcctc tcgggccccg tactcgcggt ccatgcccgt gtcctcccac
     2041 tccaccgaga gggggccagc gtagccggcc gcgttgagct cgcggatgat cgcgtcgaag
     2101 tccacgtcgc cgtggccgag ggacacgaag ttccagccgc ggcggaggtc gccgaaactc
     2161 aggtgggagc cgaggatgcc cgaacggccg tcgctcctga ccgccacgtc cttcatgtgc
     2221 acgtggtaga tccggtcggc gaagtcgcgg atgaagagct cgggccgcac gccctgccac
     2281 aaaaggtgcg aggggtcgaa gttgaagccg agggtcctgc ggtggccgaa ggcctcgagg
     2341 aggcgcttgg cggtgtagta gtcgaaggcg atctcggtgg ggtggacctc gagggcgaag
     2401 cggaccccgc actcatcgaa gacgtcgagg atcggggtcc agcggcgcac gatctcggcg
     2461 taggcctcct cgatcatcgc ctcggtggtc tgggggaagg agtaccagta gcgccagatc
     2521 ggcgagccca tgaagcaggt gacgaccgat gcgcccaagg ccttggccgc gcgggcggcg
     2581 tgcttcatgt cctcgatggc ccaggccacg atcgcctcgg gcttgccctt tacctcgggc
     2641 ggggcgaagc cgtcgagcct cgggtcccag gggtcgccga cgcactggcc ggtgaggtgg
     2701 gccgagatcg cccagacccc gaggccgtgg cgcttcagga tggccttccg ctcctcgacg
     2761 tagcgcgggt cggtggcggc cctgcggacg tcgaggtggt cgcccaggtt ggcgatctcg
     2821 atgccctcgt agccccaggc cgagaccttc tcgcagaggg tctcgaagcc gaggtcggcc
     2881 cactggccgg agatgatcgt cactggtcga gccatctgcc gctccttatt cgaactcgat
     2941 gacgcccttg atgtagccgc ccgtcctcga gagcccggcg gcgtaggcct cctcggcctt
     3001 cgcgaagctc gccacatggg tcacgaggcc ctcgttgctg atcgcccccg cggccatgag
     3061 ctttattgcg gcggggaaga gggcgtcgaa ggcgggattc atctggggct ggatgttgag
     3121 gatcctccag cccttcgtgt gccactggtc aaggtcgaat tcctggctgt ggtggtgcca
     3181 ggcgaaattc tcgatgatcg cgtagggttt ggccagggcg aaggccagct cgagcgaggc
     3241 cgccgaaccc gcggtctcga tgacgacgtc gtagacccgc tcgcgcccct ttcccggctc
     3301 cgcgcccggg gccggctcga ccgcgggggc ctggaagtcc cagcccccca tgcccgcggc
     3361 gaaggatcgg gcgagctcga ggcgacgggc atcgggctcg aagaccgaga cgcggccggc
     3421 catcgtgcgc ctcagcgcct gcagcattaa gaggcccatg tagccggcgc ccacgagggc
     3481 caccgagtcg ccgggcttga tcccggcgta gacgaggccg ttgacgacgc aggccacggg
     3541 ctcgacgacc cagcgcgtga gggggatcgt gcttccctcg gggatcgcgg agaccttgtc
     3601 cgttgccctg agcgcgtggc gggccatgag gccgcctccg cgcgagccta tggggagctc
     3661 gtggcaggcg acgcgttggc cgagctcgag gccctcgacg cgctcgccct tgcggacgat
     3721 cctgccgacg ccctcgtgtc cggcggtgaa ggggtagtcg tggaatttcg cgaagcggcc
     3781 cgagtaggcg aggaggtccc agccgcagat gccgcaggcc tccatctgaa cgaggacctc
     3841 attgggcccg gggtcctcca ggtccatcgt ccgatattcg atgcgccgct cgcccaggta
     3901 ccacaaggct tcacttttca tgccgttccc cgtgggctcc agtataaggc cgattggcca
     3961 gctgtcaatc gaaagtgttc ggccgccgaa cctttgcgtc cgggcgcggg acggtatcgc
     4021 cttgacagcg gcggcaaagg ggggataatg agccacgatg cttcatagaa tgggaagcct
     4081 cgccgaggcc gacggcagtc cggccaacgg ctcgacgacg gcgggcagca ttttcaagat
     4141 catccgcgac cacggacccc tgtacggcaa cgacatcgtc cggcgcaccg gcctggccaa
     4201 gtccacagtc tcggcctacc tcgaccgcct ggtctcggtg ggcctcgtgc gcgaggagat
     4261 ccccgagggc ggcaagcgcc gcaagctcaa ggtggccgag tcggcgggct acgtcgtcgg
     4321 cgtggccctc ggccagaccc acctcaacgt ggccctctgc gacctcgagg ccgagatcat
     4381 cgagaccgtg ggcggggagg tcgacctcgt gcgcgagtcg cccgagacgg tcctgggcaa
     4441 ggtggtggag tacacccacg agctcgagcg ccgcgcgggc ctggggcccg actcgctctt
     4501 cggcatcggg ctcggcctgc cgagccccgt cgactacgcc cggggcatgc ccgtcacccc
     4561 gccggtcatg ccgggctggg accgcttccc cgtcgcctcc ttcctagcca acgagttcac
     4621 ctgccccgtc ttcgtggaca acgacgtaaa tgtcatggcc ctcgccgagc gggacaaggg
     4681 ggccgcggcc gagctcgccg ccgactcggg ctgtttcctc ctcgtgaagg cgggcacggg
     4741 gatcggcgcg gggatcgtca taaatgggga gatctaccgg ggcgccaagg gggcggccgg
     4801 ggacatcggc cacatcggca tcgacggcga cgacaccctc tgccgctgcg ggaacgtcgg
     4861 ctgtctcgag gccgtggcgg ggggttgggc cctcgcctcc cgcgccgagc tggccgccag
     4921 gtcccagcgg agccccttcc tcgccgaggc cgtcgccgag ggcagggcca tcagccccga
     4981 gctcctcgct cgcggggccg cctcgggcga cgacgagtgc ctgaggctca tcatctcggc
     5041 gggcaaatac atcggcgacg tccttgccaa actcgtcaat ttcttcaatc cttccttcct
     5101 cgtcgtcaac ggccggctcg ccaacctggg ggagcgattc atcgcatcga tccgagagtc
     5161 ggtgtaccgg cgctccaccc ccctg
//
LOCUS       DASSQV010000506         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1861781, whole genome shotgun sequence.
ACCESSION   DASSQV010000506 DASSQV010000000
VERSION     DASSQV010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861781"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFL04_08920"
     CDS             <1..232
                     /locus_tag="VFL04_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839870.1"
                     /translation="REELLVRPSRNEEAFALHRKGFSADIIAAKLGATVAEIELLVEM
                     EERRQASFASESGLGEGPAGALGSGASRSPEA"
     gene            239..1933
                     /locus_tag="VFL04_08925"
     CDS             239..1933
                     /locus_tag="VFL04_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET7839871.1"
                     /translation="MAFAIGIDFGTTNSLCTWLDGDKPSLIPNSRGERSTPSVVAISP
                     SGQVLVGESAKNQAILNPESTVAGIKRLLGSEGRLSLGGRSWRVEEIVAFVLGSLRKD
                     AERYLEREVGAAVITAPAHFSERERRALVEAGSIAGLEVLRVVNEPTAAAVARSWNTR
                     EAEAKGTVLVYDFGGGTLDVTVLSQTGRECRVLASRGDGRLGGADLDAELCRLAASAF
                     RRDYGLEPDSDRILSQTLADAAERAKIELSEREEATIGIPFAVVGAVDGRGGRVVNPS
                     LTVTRPMFEEIALPYVERSLRLAELALAEAGLEASQVDALVLSGGSSRIPLVRRLIAE
                     RFGLRAEGRVSPAEIVALGAAAWTGILAGSERLKVIDVVSRTYGVEIDGGLFVPLIRK
                     NSPVPASSSRIFTTVADGQDSVEIHVLQGESREAGLDLSLGRFLLTGIAAAPKGKPRI
                     RVDFGIDESDILHVSALDLDTGKAQAISIADLDREGEGKDGLLRKTAALAERLEALRK
                     GLVLERGLEAELDELCERARAAALGPVAEDELRLLKVELESLVGELLARGAERGGR"
     gene            1930..2616
                     /locus_tag="VFL04_08930"
     CDS             1930..2616
                     /locus_tag="VFL04_08930"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET7839872.1"
                     /translation="MKSSEDPKAGGSGGGGEVEVLYSVLGLAPGAAEEEVRMAYRRLA
                     MRYHPDGSGDPATARQFARVVRAYKILKARASEPPRAPVARAPRDRFALVLEAEEDLF
                     SLGQILASDPDPRAREAATRRLGLSGRRAAYVFLRRAFYDSDEAVALAAVRAVAFIGS
                     RQAGGEVAALYSRSGKAMRERMLEAAAATKEPLFKATLEAAAADADPSLRARAARISR
                     ELDAALDSSP"
BASE COUNT          388 a         1001 c          993 g          367 t
ORIGIN      
        1 ccgcgaagag ctgctggtca ggcccagccg aaacgaggag gccttcgccc tccaccgcaa
       61 gggcttctcg gccgacatca tcgccgcgaa gctcggcgcg accgtggccg aaatcgagct
      121 cctcgtggag atggaggagc ggaggcaggc gagcttcgcg tccgaaagcg gcctcggcga
      181 aggcccggcc ggagctctgg gctcgggcgc atcgcggagc ccggaggcct aggcggacat
      241 ggccttcgcg atcggcatcg acttcggcac gacgaattcc ctctgcacct ggctggacgg
      301 ggacaagccc tccctgatcc cgaactcccg cggggagagg tccacgccga gcgtcgtggc
      361 gatatcgcct tcgggccagg tcctcgtcgg cgagtcggcc aagaaccagg ccatcctcaa
      421 ccctgagagc acggtggcgg ggatcaagcg cctcctcggc tcggagggca ggctcagcct
      481 gggcggccgc tcctggaggg tcgaggagat cgtggccttc gtgctcggct ccctgcgcaa
      541 ggacgccgag cgctatctcg agcgcgaggt cggggcggcc gtcatcacgg cgccggccca
      601 tttctccgag cgggagaggc gggccctcgt cgaggcgggc tcgatcgccg ggctcgaggt
      661 gctccgggtg gtcaacgagc ccacggccgc cgccgtcgcc cggtcctgga acacgcggga
      721 ggccgaggcc aaggggaccg tcctcgtcta cgacttcggg ggcggcaccc tcgacgtcac
      781 cgtgctttcc cagaccgggc gcgagtgcag ggtcctcgcc tcccgcggcg acggccgtct
      841 gggcggggcc gacctcgacg ccgagctctg ccgcctcgcg gcctcggcct tcagacgcga
      901 ctacgggctc gagcccgact cggaccgcat cctctcccag accctcgccg acgcggccga
      961 gcgcgccaag atcgagctct ccgagcgcga ggaggcgacg atcgggatcc ccttcgccgt
     1021 ggtcggggcc gtggacggcc ggggcgggag ggtggtcaac cccagcctca cggtcacgag
     1081 gccgatgttc gaggagatcg ccctgcccta tgtcgagcgg agcctcaggc tcgccgagct
     1141 cgccctcgcc gaggccgggc tcgaggcgtc ccaggtcgac gccctcgtcc tctcgggcgg
     1201 ctcgagccgc atccccctcg tgcgccgcct catagccgag cgcttcgggc tcagggccga
     1261 gggcagggtc agcccggccg agatcgtcgc cctcggagcc gcggcctgga cgggcattct
     1321 cgcgggctcg gagcgcctca aggtgatcga cgtcgtgtcc cggacctatg gggtggagat
     1381 cgacggcggg ctcttcgtcc ccctcatccg caagaacagc cccgtgccgg cctcaagctc
     1441 gaggatcttc accacggtgg ccgacggcca ggactcggtg gagatccacg tcctccaggg
     1501 cgagagcagg gaggctggcc tcgacctatc gctcgggcgc ttcctcctca cggggatagc
     1561 ggctgccccc aagggcaagc cccgcatcag ggtggacttc gggatcgacg agtccgacat
     1621 cctccacgtg agcgccctcg acctcgacac gggcaaggcc caggcgatca gcatcgccga
     1681 cctcgacagg gagggcgagg gcaaggatgg cctcctccgc aagaccgccg ccctggccga
     1741 gcgcctcgaa gccctgagga agggtctggt cctggagcgg ggcctcgagg ccgagctgga
     1801 cgagctctgc gaaagagcca gggccgcggc gctcgggccc gtggccgagg atgagctcag
     1861 gctcctcaag gtcgagctcg agagcctcgt gggcgagctc ctcgcccgcg gcgccgagcg
     1921 ggggggccga tgaagtcctc cgaggacccg aaggccgggg gctcgggcgg aggcggggag
     1981 gtcgaggtcc tctactcggt gctcgggctc gcgcccgggg ccgccgagga ggaggtcagg
     2041 atggcctacc gccgcctggc catgcgctac caccccgacg gttcggggga ccccgcgacg
     2101 gcccgccagt tcgcgcgggt ggtcagggcc tacaagatcc tcaaggccag ggcctccgag
     2161 ccgcccaggg cccctgtggc gcgcgcgccg cgggaccgtt tcgccctcgt cctcgaggcc
     2221 gaggaggacc tcttctccct cggccagatc ctcgcctcgg accccgatcc ccgcgcgagg
     2281 gaggccgcga cccggaggct cgggctgtcg ggcaggaggg cggcctacgt cttcctgagg
     2341 cgggccttct atgacagcga cgaggccgtg gccctcgccg ccgtcagggc cgtggccttc
     2401 atcggctcga gacaggcggg aggggaggtc gccgccctct acagccgctc gggcaaggcc
     2461 atgcgcgagc gcatgctcga ggccgcggcg gcgaccaagg agcccctgtt caaggccacc
     2521 ctcgaggccg cggccgccga cgccgatccc tccctgaggg ccagggccgc ccggatctcc
     2581 cgcgagctgg acgcagccct cgactcctcc ccctgaagac ggcctgctag cggatcctct
     2641 cggcgcaggc caggcccgtg ggggtcacgg ccaggccacc ctcggcgctc tccttgagcg
     2701 aggccggcat cgtggcgcct atggccttca tggcgccgac cacctcgtc
//
LOCUS       DASSQV010000507         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_368085, whole genome shotgun sequence.
ACCESSION   DASSQV010000507 DASSQV010000000
VERSION     DASSQV010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368085"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFL04_08935"
     CDS             <1..584
                     /locus_tag="VFL04_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839873.1"
                     /translation="GIVSAAQLAGQSIGISANTIIEYATDSLLVRAGLAPDSIRGLAV
                     PKIPVRMELLLAGQLKAACLPEPLLTAARVRGATLIAASDDAGLRAGVILFSKAFLDA
                     RGPDVAAFFRAYWKAAQAINADNEAYRAFLVDKAGFPEETRRAYSFVRYARPRLPSEG
                     DVAEVLAWMRGKGMLTAALEPASLLDGRALVGW"
     gene            578..1408
                     /locus_tag="VFL04_08940"
     CDS             578..1408
                     /locus_tag="VFL04_08940"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7839874.1"
                     /translation="MVRLESVSISYRIGGQELPAVQDAVAEFPSGSITAVIGPSGCGK
                     TSLIQAMAGLIRPSAGRVLIQGSELRAVRRQTAVIFQDYGLLPWKTARDNAELPLLLA
                     GMGARERHARVDPILEELGLAEFSRFFPARLSGGMRQRLAVARALASGPDLLLMDEPF
                     SSLDALTRESLQETLLAVQARHGTTVVLVTHSIEEAAYLGDSVIVMRGRNPGRIELRL
                     ASPRNSDKGPSGPVGSPGGRGADSGAFRASAAFLRLATEVRAALAGQGEEEKLEGGPA
                     "
     gene            1405..>2095
                     /locus_tag="VFL04_08945"
     CDS             1405..>2095
                     /locus_tag="VFL04_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7839875.1"
                     /translation="MKAEGALGRCLRAAAAAVFILCLWELASVAVSRPFLPSPGLALG
                     RLVSRIASGSLGPHVLASLRRVALALLLAGPLAAGLGLASGRSPRLDALVSPFVYLLH
                     PLPKVAFLPVIMLFFGLGDAAKVFLIGTIIFGQMLVSGRDAAKAVPPALVDSIRSLGA
                     GRWELLRHVVLPAALPALLSALRVCLGTAIAVLFLAETFASETGLGWYIVDAWTRVDY
                     PDMYAAIVALSL"
BASE COUNT          250 a          823 c          694 g          328 t
ORIGIN      
        1 cgggcatcgt ttcggccgcg cagctggcgg gtcagagcat cggcatctcg gccaacacga
       61 tcatcgagta cgccaccgat tccctcctcg tccgcgcggg gcttgcgccc gactcgatcc
      121 ggggcctcgc cgtgcccaag atacccgtgc gcatggagct cctcctcgcg ggccagctca
      181 aggccgcctg tctgcccgag cccctcctca ccgccgccag ggtcaggggg gccaccctca
      241 tagcggcgag cgacgacgcc ggcctccgcg ccggcgtgat cctcttctcc aaggccttcc
      301 tcgacgcccg gggccccgat gtggccgcct tcttccgcgc ctactggaag gccgcccagg
      361 ccatcaacgc cgacaacgag gcctacaggg ccttcctcgt ggacaaggcg ggcttccccg
      421 aggagacccg cagggcctac agcttcgtgc gatacgcgcg gccccgcctc ccgagcgagg
      481 gggacgtcgc cgaggtcctc gcctggatgc gcggcaaggg catgctcacc gccgcgctcg
      541 agcccgcctc cctcctcgac gggcgggccc tcgtgggatg gtgaggctcg aatcggtctc
      601 gatctcctac cggatcgggg gccaggagct tccggcagtc caggacgccg tggccgagtt
      661 cccttccggg agcatcaccg cagtcatagg cccctcgggc tgtggcaaga cgagcctcat
      721 ccaggccatg gccggcctca tcaggccctc ggcgggcagg gtcctcatcc agggctcgga
      781 gctcagggcc gtgaggcgac agaccgccgt catcttccag gactacggcc tcctgccctg
      841 gaagaccgcc cgcgacaacg ccgagctccc gctgctcctc gccggcatgg gggcccgtga
      901 gcggcacgcg cgggtcgacc ccatcctcga ggagctcggc ctcgccgagt tctcccgctt
      961 cttccccgcc cggctctcgg gcgggatgcg gcagaggctg gccgtggcgc gggccctcgc
     1021 ctccggcccc gacctcctcc tcatggacga gcccttctcg agcctcgacg ccctgacccg
     1081 cgaatccctg caggagaccc tcctcgccgt gcaggcccgc cacggcacga ccgtggtcct
     1141 ggtgacccac tccatcgagg aggcggccta cctcggggac tcggtgatcg tgatgcgcgg
     1201 ccggaacccc ggcaggatcg agctcaggct ggcctcgccc cgaaactcgg acaagggccc
     1261 ctcggggccg gtcggcagcc ccggagggcg gggcgccgac tcgggggcct tcagggcctc
     1321 ggccgccttc ctcaggctcg ccaccgaggt gagggcagcc ctcgccgggc agggagagga
     1381 ggagaagctc gagggaggcc cggcgtgaag gcggagggag ccctcggacg ctgtctgcgc
     1441 gcggcggcgg cggccgtctt catcctctgc ctctgggagc tcgcctcggt ggccgtctcg
     1501 aggcccttcc tgcccagccc cgggctcgcc ctgggccgcc tggtgtcgcg catcgcgagc
     1561 gggagcctcg gcccgcacgt cctggcctcg ctcaggcggg tcgccctggc cctcctcctc
     1621 gctggccccc tggcggccgg cctgggcctg gcctcgggcc gttcgccgag attggacgcc
     1681 ctcgtctcgc ccttcgtcta cctcctccat cccctgccga aggtggcctt cctcccggtg
     1741 atcatgctct tcttcgggct cggcgacgcc gcgaaggtct tcctcatcgg cacgatcatc
     1801 ttcggacaga tgctggtctc gggcagggac gccgcgaagg ccgtgccgcc ggccctcgtg
     1861 gactcgatcc gctccctcgg cgctggccgc tgggagctgc tccgccacgt cgtcctcccg
     1921 gccgccctcc cggccctgct ctcggccctc agggtatgcc tgggcacggc catcgccgtc
     1981 ctcttccttg ccgagacctt cgcctccgag acgggcctgg gctggtacat cgtcgacgcg
     2041 tggacgaggg tggactaccc cgacatgtac gcggccatcg tcgcgctctc gctct
//
LOCUS       DASSQV010000508         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2358931, whole genome shotgun sequence.
ACCESSION   DASSQV010000508 DASSQV010000000
VERSION     DASSQV010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358931"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VFL04_08950"
     CDS             <1..777
                     /locus_tag="VFL04_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET7839876.1"
                     /translation="YAWIKLFHALSAAFTVAAFFVMGISAINILAKRNLEVSKRSFRM
                     AAPLGLVASLLIIVAGDLSAVQVASYQPTKLAAMESVWKTEAGAPFYILQLPKLRGEG
                     NLIQALPVPKALSLLAYHDPNATVRGLEEFAPADRPSVMTAYLSFRLMVALGFLFVLL
                     AAIAIWLSRRDSLPPWFLRVMTVALGLPFLACELGWMLAEFGRQPWIVYGVLRTRDAV
                     SLSISPLDVLISLVGFLVVYSCLAAVDVFLLAKYARKEEA"
     gene            795..1820
                     /gene="cydB"
                     /locus_tag="VFL04_08955"
     CDS             795..1820
                     /gene="cydB"
                     /locus_tag="VFL04_08955"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET7839877.1"
                     /translation="MALQTLWFVLWGLLWAVYFMLDGFDFGVGMLRNFIARDETERSL
                     ALAAIGPVWNGNEVWLITAGGATFAAFPATYASMFSFLYLPMLIILFSLIARGAAVEY
                     RGKHEGARWRKAWDRVLAVSSLLAPLVLGLGFGNIFQGLPISGGIYRGTFLGLFNPYG
                     LLTALAFVALFLQHGALWLGMRTTGALADRARILAGRLWLPALALAALLLAATAAATR
                     IYDNYLAQPALFLVPLLAVASLVAVKVLHAGGRILGSFLASSATIVLVVATALVGLFP
                     NLIPSSLEAASSLTIYNSSSSQYTLGIMAVIALVFVPLVVVYQLLVYRFFRAKLDEKN
                     LAEEGGY"
     gene            complement(1958..>2585)
                     /locus_tag="VFL04_08960"
     CDS             complement(1958..>2585)
                     /locus_tag="VFL04_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7839878.1"
                     /translation="LNGSMISKLRIPPFIATLGMMTFARGWAFIYSQGRPRGSLTDGF
                     QALGQGMVFGIPNPALVLLVCVAITYVILQHTLWGRYTYALGSNLQAAVVSGIKTNRY
                     LILVYTYAGFLAAVAAIVLTARIGSGQPGLGLGYELDAIAASVIGGTSLAGGLGSAGG
                     TLIGALIIGVMKNGLDILNVSADYQQIARALIIVIAVSIDVRKNVRRS"
BASE COUNT          349 a          941 c          816 g          479 t
ORIGIN      
        1 tatgcctgga tcaagctctt ccacgccctg agcgccgcct tcaccgtggc ggccttcttc
       61 gtcatgggca tctcggccat caacatcctc gcgaagcgca acctcgaggt ctcgaagcgc
      121 tccttccgca tggccgcccc cttgggcctc gtggcctccc tcctcatcat cgtcgcgggc
      181 gacctctcgg ccgtccaggt ggcgagctac cagcccacga agctggccgc gatggagtcg
      241 gtctggaaga ccgaggccgg agcccccttc tacatcctcc agcttcccaa gctccggggc
      301 gagggcaacc tcatccaggc cctccccgtc ccgaaggccc tgagcctcct cgcctaccac
      361 gatccgaatg cgacggtgcg gggcctcgag gagttcgccc cggccgacag gccctcggtc
      421 atgaccgcct atctcagttt caggctcatg gtggccctcg gcttcctctt cgtcctcctg
      481 gcggccatcg cgatctggct ctcgaggagg gacagtctcc cgccctggtt cctccgggtc
      541 atgacggtgg ccctcggcct gcccttcctc gcctgcgagc tgggctggat gctcgccgag
      601 ttcggccgcc agccctggat cgtctacggg gtccttcgga cgagggacgc ggtctccctc
      661 agcatctccc ccctcgacgt cctcatatcc ctggtcggct tcctggtggt ctactcctgc
      721 ctggccgccg tcgacgtctt cctcctcgcg aagtacgcga ggaaggaaga agcctaacac
      781 aaggaggcgc gaaaatggcg ctgcagaccc tctggttcgt gctctggggc ctcctctggg
      841 ccgtttactt catgctcgac ggcttcgact tcggggtggg catgctgcgg aacttcatcg
      901 cccgcgacga gaccgagcgc tccctcgccc tggcggccat cggccccgtg tggaacggga
      961 acgaggtctg gctcatcacg gcgggcgggg cgaccttcgc cgccttcccc gcgacctacg
     1021 cctcgatgtt cagcttcctc taccttccga tgctcatcat cctcttttcg ctcatcgcgc
     1081 ggggagcagc tgtggagtac cgcgggaagc acgaaggggc ccggtggagg aaggcctggg
     1141 acagggtcct cgccgtctcg agcctcctcg cccccctcgt cctcggcctc ggcttcggca
     1201 acatcttcca gggcctcccc atctccgggg gcatctaccg cgggaccttc ctcgggctct
     1261 tcaaccccta tggcctcctg accgccctgg ccttcgtcgc cctcttcctc cagcacggcg
     1321 ccctgtggct cggcatgagg acgacaggcg ccctggccga tcgcgcgagg atcctggccg
     1381 ggaggctctg gctccccgcc ctcgccctgg ccgccctcct cctcgccgca acggctgccg
     1441 ccacgaggat ctacgacaac tatctcgccc agcccgccct cttcctcgtc cccctcctcg
     1501 cggtggcgag cctcgtcgcg gtgaaggtcc tccacgcggg gggccgcatc ctcggctcct
     1561 tcctcgcctc ctcggccacc atcgtcctcg tcgtggcgac cgccctcgtg ggcctgttcc
     1621 cgaacctcat cccctcgagc ctcgaggcgg cctcgagcct gaccatctac aactcctcct
     1681 cgagccagta cacccttggc atcatggcgg tcatcgccct ggtcttcgtg cccctcgtgg
     1741 tcgtctacca gctcctggtc taccgcttct tcagggccaa gctggacgag aagaacctcg
     1801 ccgaggaggg cggctactga gggcctagat ggatctcccg tggatcgatg ccggcgcccg
     1861 ctctgggcgc cggttctttt ggctgcggcg gacccggcgc ggccaggctg cccgcccggg
     1921 gtgccggctc tctttccctt ggcggactcg ccccggccta gctcctcctc acgttcttcc
     1981 tcacgtcgat gctcaccgcg atgacgatga tgagggcgcg ggcgatctgc tggtagtcgg
     2041 ccgagacgtt gaggatgtcc aggccgttct tcatgacgcc gatgatgagg gcgccgatga
     2101 gggtgccgcc cgcgctgccg aggcctccgg ccaggctcgt gcccccgatg accgaggcgg
     2161 cgatggcgtc gagctcgtag ccgaggccga ggccgggctg gcccgagccg atgcgcgccg
     2221 tgagcacgat ggccgccaca gcagcgagga agcccgcgta ggtgtacacg aggatgaggt
     2281 agcggttggt cttgatgccc gagacgacgg ccgcctggag gttcgagccg agggcgtagg
     2341 tgtagcggcc ccagagtgtg tgctggagga tcacgtaggt gatggccacg cacacgagga
     2401 ggacgagggc ggggttgggg atcccgaaga ccatgccctg gcccagggcc tggaagccgt
     2461 cggtgaggct ccctcggggc cggccctggg agtagatgaa ggcccaaccg cgggcgaagg
     2521 tcatcatgcc caatgtggcg atgaagggcg ggatcctgag cttcgagatc atggagccgt
     2581 tgagg
//
LOCUS       DASSQV010000509         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1486593, whole genome shotgun sequence.
ACCESSION   DASSQV010000509 DASSQV010000000
VERSION     DASSQV010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486593"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1493
                     /locus_tag="VFL04_08965"
     CDS             72..1493
                     /locus_tag="VFL04_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET7839879.1"
                     /translation="MTEGNEARLIVAFSIPLLLGNVFQQLYSTVDAMVVGIGVSKEAL
                     AAIGVSLPLICLTISVIMGVSMGGSVVMSQYFGAREPVHLRAAMHTIYAFLLVASVIT
                     SVLGIALADPILVFIGTPAAYLDQARVFLQITFSGMVFVFGYNAISSVLRSLGDSRNP
                     LYFLVFASLLNIGLVLLFVLVLGWGVAGSAWATFFAQAAAFAASLVFVQRSKDELLHI
                     SLRELRIDPEELRRILRVGLPTALQMGLVALSFVALSAIVNPFGTDVMAGYAAASRLD
                     SFAVLPSMNLSIAMSIFVGQNMGARRPERVRRALRATLVLSTALSLVISALFLLIPDS
                     LNRLFATDPAVVAHGARYLVIVASFYPLFSAMFVLSGLLRGAGAATFAMASAVVAVWA
                     ARIPASFVLSRIYGADGIWIGIPVGWVIGLGMVAVKYLGGSWKDYRVIGDEAAEGLVA
                     EAGELAVASEGKDLPCGYGLAEC"
     gene            complement(1503..>2730)
                     /locus_tag="VFL04_08970"
     CDS             complement(1503..>2730)
                     /locus_tag="VFL04_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839880.1"
                     /translation="DWADEDPKWFEEARRSYATLIQRYGGDDLYLGRMLRYFIRTDNL
                     KEILPLKARFLGERRNPLDALSMAELGGYLMDKGMLDDVSVLLEEAAKRDPTVPETHF
                     ELARYFRHTASTGEERKALDNAVRTYRALPALTTKRTAGYIESLIWRGRFRIAEGQWI
                     GAEEDFTAAAAAYEDAVGLRRIPRGAHFHETFAGEAYAGLAEVAFRQRDDLASALQLY
                     ERAAANGYDTADSRYRRGYILYRTGRYAEALEQLYTAGRMGSEGPYLSFAFGAALYAR
                     EDWSAAEGYFRKVKDMVSDELAGIDSPAPREDSHQAELVTLLMKADNNLGAALFNVAN
                     RLGNARLRAQAYASFAEATRSYDELGLGSAEQAKPEDRNLGLLNMNLALNPRRGVSPV
                     VYSEIEQDPSFPKKQL"
BASE COUNT          365 a          915 c          913 g          537 t
ORIGIN      
        1 caacgcgccc gaggagtcct cgtccttttc tagacccttc aaaaatgcgt attttcggga
       61 acatgcgtac catgaccgaa ggaaacgagg cgcgcctgat cgtcgccttc tcgatacccc
      121 tcctcctggg aaacgtgttc cagcagctct actccaccgt cgacgccatg gtggtgggca
      181 tcggcgtgag caaggaggcc ctcgccgcca tcggggtcag cttgcccctg atctgcctga
      241 ccatctcggt gatcatgggg gtgtccatgg gcggaagcgt ggtgatgtcc cagtatttcg
      301 gcgcccgcga gcccgtccac ctcagggccg ccatgcacac gatctacgcc ttcctcctcg
      361 tcgcgagcgt catcacctcc gtgctgggta tcgcactcgc ggaccccatc cttgtcttca
      421 tcgggacccc ggccgcctat ctggaccagg cgagggtctt cctccagatc accttctccg
      481 gcatggtctt cgtcttcggc tacaacgcga tctcctcggt gctccgctcc ctcggcgact
      541 cgaggaaccc cctctatttc ctggtcttcg ccagcctcct caacataggc ctggtgctgc
      601 tcttcgtgct tgtcctcggc tggggcgtgg cgggctcggc ctgggccacc ttcttcgccc
      661 aggcggcggc cttcgcggcc tccctcgtgt tcgtccagcg ctccaaggac gagctcctcc
      721 acatctcgct gagggagctc aggatcgatc ccgaggagct gcggaggatc ctcagggtcg
      781 gtctgcccac agccctccag atgggccttg tcgccctgtc cttcgtggcc ctgagcgcga
      841 tcgtgaaccc cttcgggacc gatgtcatgg cgggctacgc cgctgcgagc aggctcgaca
      901 gcttcgccgt cctcccttcg atgaacctga gcattgccat gagcatcttc gtcgggcaga
      961 acatgggggc gcgcagacca gagcgcgtgc gcagggccct gcgcgccacc ctcgtcctct
     1021 ccaccgccct gtcgcttgtg atctcggccc tcttcctgct tatcccggac tctctcaaca
     1081 ggctcttcgc caccgacccg gcagtggtcg cccatggcgc gcgctacctc gtcatcgtcg
     1141 cctccttcta tcccctcttc tcggcaatgt tcgtgctgag cggactgctc aggggggctg
     1201 gggcggcgac cttcgcgatg gcctcggccg tcgtggcagt gtgggctgcg cggatcccgg
     1261 cgagttttgt cctgtcgcgc atctacgggg ccgacggcat atggatcggg atcccagtcg
     1321 gctgggtgat tggcctcggc atggtggcgg tgaagtacct cggggggtcc tggaaggact
     1381 accgggtcat aggggacgag gccgctgagg gcctcgtcgc cgaggcgggg gagctcgccg
     1441 tcgcgtccga gggcaaggac ctgccctgcg gctatggcct ggccgagtgc tgatccagcc
     1501 ctttagagct gctttttcgg gaagctgggg tcctgctcga tctcggagta gacgacgggg
     1561 gagacgccgc ggcgggggtt tagggccagg ttcatgttga ggaggccgag gttcctgtcc
     1621 tcgggtttgg cctgctcggc gctcccgagg cccagctcgt cgtaggacct cgtggcctcg
     1681 gcgaaggagg cgtaggcctg ggcgcgcagc ctggcgttcc caaggcggtt cgcgacgttg
     1741 aagagggcgg ccccgaggtt gttgtcggcc ttcatgagga gggtgacgag ctcggcctgg
     1801 tgggaatcct ccctgggcgc tggcgagtcg atccccgcga gctcatcgct gaccatgtcc
     1861 ttcaccttcc ggaaataacc ctcggcggcc gaccagtcct cgcgggcgta gagggcggcg
     1921 ccgaaggcga agctcagata ggggccctcg ctgcccattc ggccagccgt gtacagctgc
     1981 tcgagcgctt cggcgtagcg gcccgtccgg tagaggatgt agccccgcct gtacctggaa
     2041 tcggcggtgt cgtagccgtt ggccgccgcg cgctcgtaga gctggagggc cgaggcgagg
     2101 tcgtccctct ggcggaatgc cacctcggcg aggccggcgt aggcctcgcc ggcgaaggtc
     2161 tcgtggaaat gggcgccgcg cgggatccgc ctcaagccga ccgcgtcctc gtaggccgcg
     2221 gccgcggcgg tgaagtcctc ctcggccccg atccactggc cctcggctat cctgaaccgg
     2281 ccccgccaga tgaggctctc gatgtagccg gccgtccgct tcgtcgtgag ggcgggcagg
     2341 gccctgtagg tcctgaccgc gttgtcgagg gccttcctct cctcgccggt cgaggcggtg
     2401 tggcggaagt agcgggcgag ctcgaaatgg gtctcgggga ccgtcgggtc ccgcttggcg
     2461 gcctcctcga gaaggacgct gacgtcatcg agcatgccct tgtccatgag gtagccgccg
     2521 agctcggcca tggagagggc gtcgaggggg ttcctcctct cgccgaggaa gcgggccttg
     2581 aggggcagga tctccttcag gttgtcggtc ctgatgaagt agcgcagcat cctcccgagg
     2641 tagaggtcgt cgccgccgta gcgctggatg agggtggcgt agctccgcct cgcctcctcg
     2701 aaccacttcg ggtcctcgtc ggcccagtcc
//
LOCUS       DASSQV010000510         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_244724, whole genome shotgun sequence.
ACCESSION   DASSQV010000510 DASSQV010000000
VERSION     DASSQV010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244724"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFL04_08975"
     CDS             complement(<1..500)
                     /locus_tag="VFL04_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839881.1"
                     /translation="MKVSRAWIGHGDARFRAGLRDLGRHRPDLAVRRFREAADSCPAS
                     RPGLLSRRLYWLATALLRLDQTELALKSLASAQKLRPRGYAREAYSRRVNAYGMPRRQ
                     SPELDDFYAFYSIHMCEYLRKKEQPRFDTRQEKDVVVRLVGDSWLALSRSRRLAALGA
                     GEKLRL"
     gene            complement(542..>2314)
                     /gene="glgX"
                     /locus_tag="VFL04_08980"
     CDS             complement(542..>2314)
                     /gene="glgX"
                     /locus_tag="VFL04_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET7839882.1"
                     /translation="SPDKDLSFSAVDDAAAVPKCVVVSDEFDWRGDRPLNYPLRHCVI
                     YETHVRGLSLHPTSGVDHPGTYRGVTEMIPYLKGLGVTSLELLPVQEFDEFENPRSNP
                     LTGERLSNYWGYSTIAFMAPKGSYSSAGSLGEQVAEFKEMVRELHAAGIEVILDIVFN
                     HSAEGNELGPTLNFRGLDNRIYYILDEDPRFYKNYSGCGNTLNCNHPVMRSLIMDCLH
                     YWVIDMHVDGFRFDLGSILGRDHRGRLLENPPVIERIAEDPVLRHTKIIAEAWDAGGA
                     YQVGWFPGGRWAEWNDRYRDDVKRFWKGETGAVRNFATRITGSSDLYLRDGRKPFHSI
                     NFVTAHDGFTLNDLVSYERKHTEANGEGNADGLDANISSNYGVEGPSRNAALDAVRNR
                     QVKNFLGTLLLSIGTPMLLGGDEFRRTQNGNNNAYCQNNEIGWYDWECLKTHADIQRF
                     TRAVLAFRMRHPAFRRPEFFTGKDSDFNAIPDIGWFDETGGPPDWARIERRIAARIDG
                     SRADILTDRDDNDFYLIFNAAPESAVFKIGPAPMSRRWHRAIDTSLPSPEDIVEPGDE
                     PIVAPQDEYAVAPRSLVVLISCPD"
BASE COUNT          387 a          706 c          797 g          424 t
ORIGIN      
        1 aacagcctga gcttctcccc cgcgccgagg gcggcgagcc gtctggagcg ggacagggcg
       61 agccaggaat ccccgaccag cctgaccacg acatctttct cctgcctggt gtcgaagcgt
      121 ggctgctcct tcttcctcag atactcgcac atgtggatgg aatagaacgc atagaagtcg
      181 tcgagctcgg ggctctggcg tctcggcatc ccgtaggcgt tcaccctgcg gctataggct
      241 tctctcgcgt agccgcgggg ccgcagcttc tgagccgagg ccaggctctt cagggcgagt
      301 tcggtctggt cgagccggag cagggcagtg gcgagccagt aaagtcgccg cgacagcagg
      361 ccgggccgcg aggcggggca ggaatcggcg gcttccctga acctgcggac cgcgagatcg
      421 ggtctgtggc gccccagatc gcgcaaacct gcgcgaaacc tcgcatcgcc atggccgatc
      481 cacgctctgc tcaccttcat ggtattaaag tacgtctgta cgatttcgga agcaagtcag
      541 atcaatcggg gcacgatatc agcaccacaa gggaccgcgg cgcgacggcg tactcgtcct
      601 ggggcgcgac gatcggctca tcccccggct ccacgatgtc ctccggcgag ggcagggaag
      661 tgtcgatcgc ccggtgccag cgcctcgaca tcggcgcggg cccgatcttg aagacggccg
      721 actcgggggc ggcgttgaaa atcaggtaga agtcgttgtc gtcccggtcg gtgaggatgt
      781 cggcgcggga gccgtcgatg cgggcggcga tcctgcgctc gatgcgcgcc cagtcaggcg
      841 gccccccggt ctcgtcaaac cagcctatgt cagggatggc gttgaagtcc gagtccttgc
      901 cggtgaagaa ctcgggcctc cggaaggcgg gatgcctcat gcggaaggcc aggacggccc
      961 tcgtgaagcg ctggatgtcg gcgtgggtct tcaggcattc ccagtcatac cagccgatct
     1021 cattgttctg gcagtacgcg ttgttgttgc cgttctgggt cctgcggaac tcgtcgccac
     1081 cgaggagcat cggagtcccg atggagagga ggagggtgcc gaggaagttc ttcacctggc
     1141 ggttccggac cgcgtcgagg gcggcgttcc tcgagggccc ctccaccccg tagttcgagg
     1201 agatgttcgc gtcgaggccg tcggcgttgc cctcgccatt cgcctcggtg tgcttacgct
     1261 cgtagctgac caggtcgttc agggtgaagc cgtcatgggc ggtcacgaag ttgatcgagt
     1321 ggaaaggttt gcggccgtcg cgcaggtaga ggtcgctcga gccggtgatg cgtgtagcga
     1381 agttgcgcac ggccccggtc tcgcccttcc agaagcgctt cacgtcgtcg cggtagcggt
     1441 cgttccactc ggcccagcgt ccccccggga accagcccac ctgataggcc ccgcccgcat
     1501 cccaggcctc ggcgatgatc ttcgtgtgtc tgaggacggg gtcctcggct atcctctcga
     1561 tcacgggagg attctcgagg aggcggcccc tgtggtccct gccaaggatc gagccgaggt
     1621 cgaagcggaa gccgtcgacg tgcatgtcga tgacccagta gtgcagacag tccatgatga
     1681 gcgaccgcat gacggggtgg ttgcagttca gggtgttgcc gcagcccgag tagttcttgt
     1741 agaagcgggg gtcctcgtcg aggatgtagt agatcctgtt gtccagaccc cggaagttca
     1801 gggtcgggcc gagctcgttg ccctcggcgg agtggttgaa gacgatgtcg aggatcacct
     1861 cgatgccggc ggcatggagc tccctcacca tctccttgaa ctccgccacc tgctcaccca
     1921 gggagcccgc ggaggaatag ctccccttgg gagccatgaa ggcgatggtc gaatagcccc
     1981 agtaattgga caggcgctcc cccgtcaggg ggttgctgcg ggggttctcg aactcgtcga
     2041 actcctgcac gggcaagagc tcgaggctgg tgaccccgag gcccttgaga taggggatca
     2101 tctcggtgac gccgcggtag gtcccgggat gatccacacc cgaggtgggg tggagactca
     2161 ggccccggac atgggtctcg tagatgacgc agtggcgcag ggggtagttc agtggcctgt
     2221 cgccgcgcca gtcgaactcg tccgacacga cgacgcactt gggcactgcg gccgcgtcgt
     2281 ccacggccga gaatgagagg tccttgtcgg ggga
//
LOCUS       DASSQV010000511         3477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2234221, whole genome shotgun sequence.
ACCESSION   DASSQV010000511 DASSQV010000000
VERSION     DASSQV010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2234221"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..2476
                     /locus_tag="VFL04_08985"
     CDS             137..2476
                     /locus_tag="VFL04_08985"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7839883.1"
                     /translation="MSRILESELNVAGLTSYIDDLGEPRGMLHGTLVVSPCARGLLLG
                     IDASAALALHPSVRVLTAKDVPGENQLGASVPDEPLLAEGEWGFKGQGLALVLAETRS
                     LARKAAALVLPSYEELEPLLDAREASRKGSYILPSRTMAAGDTASAFARSAIVVEGST
                     ESGGQEHVYLETQGAIAEPREGGRVFVLSGTQHPSGVQHAVARVLGLSMNAVEVETRR
                     LGGGFGGKEDQAAGWAALAALGAFVTGRQVKVYLNRREDMRWTGKRHPYSADFRMGLD
                     AEGNILAFEADYYQNSGAACDLSPAILSRTLFHAASAYRVPNLRVTGRMCRTNLVPFT
                     AFRGFGAPQGIFVMECALDAAARAGGWDPVELRRRNLLREGDVFHFGQPLANCHTERS
                     MDRLLQKAGWDTLRARIDSYNASHALAKKGAAILPLAFGVSFTKLVMNQGGALVHVFH
                     DGSVSVSTGAVEMGQQVARKIQVVAARALGISESRVRVESTRTGTVANTFPTAASTGS
                     DINGMAALAACEEIRGRLLAFAAKTLGAELGELRLEGELVVKGCEPTDLGWERLVDEA
                     HLARVDLSAHGFYATPGLFYDMKAERGSPFAYHVYGCAAVTATLDVLRGTYSFDRATI
                     VHDAGESLDLAVDLGQVEGAFAQGLGWSALEDLRFDAKGSLLTDTLSTYKLPDVHFMP
                     EMEVEFLADAGNPKAVMRSKAIGEPPFMYGIAGFFAVLDALRAARPDQAPAYDLPMKP
                     EKALAVLAAPRAPGAKGPGPAERPASGKSRPECAEGAPA"
     gene            2473..>3477
                     /locus_tag="VFL04_08990"
     CDS             2473..>3477
                     /locus_tag="VFL04_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7839884.1"
                     /translation="MIIANCLAALPGEAEPRRVSIRVRDGRFEAIGAGLRPEPGEEMV
                     EAEGLLLLPGAIDPHVHFDEPGFTHREDFLHGTMEAAKGGVTTVIDMPCTSLPPVTTA
                     AALRNKLEAIRGRAVVDYALFGGVEGGSLEASLSGAMAELSPGVVGFKCYFISGMETF
                     RRVSHADFPRIIAEGARLGRPVLLHAEDLDYVTAATARVRARRGGARAEWADYAESRD
                     EAAELVAVASALALARGREASLHVVHVGTAAAAAALAAAGASCETCAHYLAFTREDFP
                     RLGASLKTAPVVKSAAEREALWGLLAEGRIAFLASDHAPAPEAEKNTGDIWTAYGGIP
                     G"
BASE COUNT          492 a         1372 c         1146 g          467 t
ORIGIN      
        1 tggccgagat ctcccccctc gccgacgccc gcggcggcgc cgagtaccgg aggcaggtca
       61 tgggacgcct cgtccttggc cacttcctgg ccgtcttccc cgagctcgag cccgagctcg
      121 cgaaggaggt cctcgcatga gccgcatcct cgagtccgag ctcaacgtcg ccggcctcac
      181 ctcctacatc gacgacctcg gcgagccccg tggcatgctc cacgggaccc tcgtcgtctc
      241 gccctgcgcc cgcggccttc tccttggcat cgacgcctcg gccgccctcg ccctccaccc
      301 ctcggtccgg gtcctcacgg ccaaggacgt gcccggcgag aaccagctcg gggcctcggt
      361 gcccgatgag cccctcctcg ccgagggcga gtggggcttc aagggccagg gcctcgccct
      421 cgtcctcgcc gaaacgaggt ccctggcccg gaaggccgcc gccctcgtcc tgccgagcta
      481 cgaggagctc gagccgctgc tcgacgccag ggaggcctcg aggaagggct cctacatcct
      541 cccctcgcgc accatggccg cgggggacac ggcctcggcc ttcgcccgct ccgccatcgt
      601 ggtcgagggc tcgaccgagt ccggcggcca ggagcacgtc tacctcgaga cccagggcgc
      661 catagccgag cccagggagg gagggagggt cttcgtgctc tcgggcaccc agcacccgag
      721 cggggtccag cacgccgtgg cccgggtcct cggcctttcg atgaacgccg tcgaggtcga
      781 gacccgccgc ctcggcgggg gcttcggcgg caaggaggac caggccgccg gctgggctgc
      841 cctcgcggcc ctgggcgcct tcgtgacggg aaggcaggtc aaggtctacc tgaaccgccg
      901 cgaggacatg cgctggaccg gcaaacgcca cccctactcg gccgacttcc gcatgggact
      961 cgacgccgag ggcaacatcc tcgccttcga ggccgactac taccagaact cgggcgcggc
     1021 ctgcgacctc tcgcccgcca tcctctcgcg caccctcttc cacgccgcga gcgcctatcg
     1081 cgtgcccaac ctcagggtga ccggcaggat gtgccgcacc aacctcgtcc ccttcaccgc
     1141 cttccgcggc ttcggcgccc cccagggcat cttcgtcatg gagtgcgccc tcgacgccgc
     1201 ggcccgcgcg ggaggctggg accccgtcga gctgcgccgg aggaacctcc tccgcgaggg
     1261 cgacgtcttc cacttcggcc agccactggc caactgccac accgagcgct ccatggacag
     1321 gctcctccag aaggcaggct gggacactct gcgcgcccgg atcgattcgt acaacgcgtc
     1381 ccacgccctc gcgaaaaagg gcgccgccat cctccccctc gccttcgggg tctccttcac
     1441 caagctcgtg atgaaccagg gcggggccct cgtccacgtc ttccacgacg gctcggtctc
     1501 ggtctcgacg ggggccgtgg agatgggcca gcaggtggcg cgcaagatcc aggtggtcgc
     1561 ggcgagggcc ctcgggatct ccgagtccag ggtcagggtc gagtcgacca ggacgggcac
     1621 agtggccaac accttcccca cggccgccag cacgggctcg gacatcaacg gcatggccgc
     1681 actcgcagcc tgcgaggaga tccgaggccg cctcctcgcc ttcgccgcca agaccctcgg
     1741 ggccgagctc ggcgagctca ggctcgaggg cgagctcgtg gtgaagggct gcgagcccac
     1801 cgaccttggt tgggagaggc tcgtggacga ggcccacctc gcgcgggtcg acctctcggc
     1861 ccacggcttc tacgccaccc ccggcctctt ctacgacatg aaggccgagc ggggcagccc
     1921 cttcgcctac cacgtctacg gctgcgccgc ggtgaccgcc accctcgacg tcctgcgcgg
     1981 gacctacagc ttcgaccggg ccaccatcgt gcacgacgcc ggcgagagcc tcgacctcgc
     2041 cgtggacctc ggccaggtcg agggcgcctt cgcccagggc cttggctggt cagccctcga
     2101 ggacctgcgc ttcgacgcga agggctccct cctcaccgac accctctcca cctacaagct
     2161 ccccgacgtc catttcatgc ccgagatgga ggtcgagttc ctcgccgacg cgggcaaccc
     2221 caaggccgtg atgcgctcga aggccatcgg cgagcctccc ttcatgtacg gcatcgccgg
     2281 cttcttcgcc gtcctcgacg ccctgcgcgc cgcgaggcca gaccaggccc cggcctacga
     2341 cctgcccatg aagccagaga aggccctcgc cgtcctcgcg gcgccgcggg ccccgggcgc
     2401 caaggggccc gggccggcgg aacggcccgc ctccggcaag agccggcccg aatgcgccga
     2461 gggggccccg gcatgatcat cgccaactgt ctcgcggccc tgcccggaga ggccgagccg
     2521 cgccgagtct ccatccgcgt gcgcgatggc cgtttcgagg cgatcggagc cgggctcagg
     2581 cccgagcccg gcgaggaaat ggtcgaggcc gagggcctcc tcctcctccc cggggccatc
     2641 gacccccacg tccacttcga cgagccgggc ttcacccacc gcgaggactt cctccacggg
     2701 acgatggagg ccgccaaggg aggggtgacg accgtgatcg acatgccctg cacctccctg
     2761 ccaccggtca cgacggccgc ggccctccgc aacaagctcg aggcgatccg cggcagggcc
     2821 gtggtcgact acgccctctt cggcggggtc gagggcggga gcctcgaggc gagcctctcc
     2881 ggcgcgatgg ccgaactcag tccaggggtc gtcggcttca agtgctactt catctcgggc
     2941 atggagacct tcaggcgggt cagccacgcc gacttccccc gcatcatcgc cgagggcgca
     3001 aggctcggcc gtcccgtcct cctccacgcc gaggacctcg actacgtgac cgccgccacg
     3061 gcccgggtca gggcgaggcg gggcggagcc agggccgagt gggccgacta cgccgagagc
     3121 cgggacgagg cggccgagct cgtggccgtc gcctcggccc tcgccctcgc gcggggccgc
     3181 gaggcgagcc tccacgtcgt ccacgtgggc acggccgccg ccgccgcggc cctggccgcc
     3241 gcgggggcga gctgcgagac ctgcgcccac tacctcgcct tcacgcggga ggacttcccc
     3301 cgcctcggcg cctcgctcaa gacggctccc gtggtgaagt ccgcggccga gcgcgaggcc
     3361 ctctggggcc tcctcgccga ggggaggatc gccttcctcg cgagcgacca cgccccggcc
     3421 cccgaggccg agaagaacac gggcgacatc tggaccgcct acggcggcat cccgggc
//
LOCUS       DASSQV010000512         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_616783, whole genome shotgun sequence.
ACCESSION   DASSQV010000512 DASSQV010000000
VERSION     DASSQV010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616783"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VFL04_08995"
     CDS             <1..644
                     /locus_tag="VFL04_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839885.1"
                     /translation="DLQNPITVTLDGPTTIAQGASMTVSATSSEPVDSYQWYLQGSPV
                     GTNSSSIVLGSSLALGHYWLSLVVTKGNILSSKSVEFDVLPSLIRNYQLTLNVQSGSY
                     YGAVYHGTFSVDESALTGVGVEVVPNGMRNFLFYPFGGSYGPANGGDVIYVNGVIDKI
                     NHTGTVNGRGWGFNIGFEDYQVPPDIFPGRWWGYLDVYTFVEGFGTYTLTQVM"
     gene            817..2115
                     /locus_tag="VFL04_09000"
     CDS             817..2115
                     /locus_tag="VFL04_09000"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7839886.1"
                     /translation="MSERRPAPSPRPAWRRVFVSFILSQTLSLLGSSLVQYAIVWYVT
                     LRTQSGLMMTLITVFAFLPAFFLSPFAGVWADRHDRKRIIMLADGGIALVTLALALLF
                     MAGQQGIWLLLLASALRAVGGAIQGPAVGAILPQIVPEGELLRANGIVGTIQSTIMLV
                     SPMASGALLTFAPIQTVFFIDVATAALAIGVLLFIVRIAPHAKALEKAEAGYFADMRA
                     GLRYIREHRYLTTFFTYLALFLFMITPAAFLTPLQVTRNFGAEVWRLSAIEIAFSAGM
                     MLGGAAISIWAGFRNRMRTMVFANLAMALCTIALGLLPWFWPYIAVMGIFGIALPFFN
                     SPSATMIQEHVEQDFLGRVFSVFTMLQTSLMPLGMLLFGPLADRIRVEWLLLGTGAAM
                     LAHGLVALGNRRLIETGEPIALAAANAGTGAAAEEAPIDA"
BASE COUNT          364 a          817 c          666 g          432 t
ORIGIN      
        1 ccgatctcca gaacccgatc accgtgaccc tcgacggccc gacgaccatc gcgcagggcg
       61 ccagcatgac ggtgtcggcg acgagctcgg agcccgtgga cagctaccag tggtacctcc
      121 aaggctcgcc ggtcggcacg aactccagct ccatagtcct tgggtcgagc ctcgccctcg
      181 gccactactg gctgagcctc gtcgtcacca agggcaacat cctgagctcc aaaagcgtgg
      241 agttcgacgt cctgccatcc ctcatccgca actatcagct caccctcaac gtgcaatccg
      301 gaagctacta cggcgcggtc taccacggaa ccttctcggt cgacgaatcg gccctgacgg
      361 gggtcggcgt cgaggtcgtg cccaacggga tgaggaattt cctgttctat cccttcggcg
      421 gcagctacgg tccggcgaac ggcggcgacg tgatctacgt caacggcgtg atcgacaaga
      481 tcaaccatac gggcacggtg aacggtcggg gctggggctt caacatcggc ttcgaggact
      541 atcaggttcc tcccgacatc ttccccggcc gctggtgggg ctatctcgac gtctatacct
      601 ttgtggaagg cttcgggacc tacaccctga cccaggtgat gtagtaatca ggaatcctcc
      661 tgtcgccccc gcggatgccc gccgtttcta gccgggaagc gggccttcgc ggggcgccgg
      721 agtgccgggt gggcgcggct tcagcgccca cccgggcttt cccgagccca ggggcggggc
      781 ctagtgtcct gcgcccgatt ccgctatttt gtggccatgt ccgagagaag gcccgctccg
      841 agtccacgtc ccgcctggcg ccgcgtcttt gtcagtttca tcctgagcca gaccctctcc
      901 ctcctcggtt cctcgctcgt gcagtacgcc atcgtctggt acgtgactct ccggacccag
      961 tccggtctga tgatgaccct gatcacggtc ttcgccttcc tgccggcctt cttcctctcg
     1021 cccttcgcgg gggtctgggc cgaccgccac gaccggaagc gcatcatcat gctcgcggac
     1081 ggaggcatcg cccttgtcac cctggccctc gccctcctct tcatggcggg acaacagggc
     1141 atctggctcc tactcctcgc ctcggccctc agggccgtcg ggggcgccat ccaggggccc
     1201 gccgtcggcg ccatccttcc ccagatcgtg ccggagggcg agctcctccg agcgaacggc
     1261 atcgtcggca ctatccagtc gaccatcatg ctcgtctcgc ccatggcaag cggggccctc
     1321 ctcacctttg cgccgatcca gacggtcttc ttcatcgatg tggcgacggc ggccctcgcc
     1381 atcggggtcc tgcttttcat cgtgaggatc gcgccccacg ccaaggccct cgagaaggcc
     1441 gaagcgggct acttcgccga catgcgggcc ggactccgct atatccggga gcaccgctac
     1501 ctcacgacct tcttcaccta cctcgcgctt ttcctcttca tgatcacgcc ggcggccttc
     1561 ctcacccccc tgcaggtcac gaggaatttc ggcgccgagg tctggcggct ctcggccatc
     1621 gagatagcct tctcggcggg catgatgctc gggggagccg cgatctcgat ctgggccggc
     1681 ttccgcaacc gcatgcggac gatggtcttc gccaacctcg cgatggccct ctgcacgatc
     1741 gccctcggcc tcctgccctg gttctggccc tacatcgcgg tcatggggat cttcggcatc
     1801 gccctgccct tctttaacag cccctcagcg acaatgatcc aggagcacgt ggagcaggac
     1861 ttcctcggcc gagtcttcag tgtcttcacc atgctccaga cctcacttat gcccctcggc
     1921 atgctcctct tcggccccct cgccgacagg atccgggtcg agtggctcct cctcggcacg
     1981 ggggcagcaa tgctcgccca tggccttgtc gccctcggca accggcgcct catcgagacg
     2041 ggagagccga tcgcgctcgc ggccgcgaat gcgggtaccg gggccgccgc cgaagaggcc
     2101 ccaatcgacg catgagccta gcggcgggac aagggcggcc cagcgtgata tagtcgcctt
     2161 cccagggaga acacgaatga aagcgctgca tccagactcg aaggccaatg atctcgcgaa
     2221 gatgtacgaa cagagctttt ccggaatggg ctactataag cccggccaag ccgtggaga
//
LOCUS       DASSQV010000513         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_866997, whole genome shotgun sequence.
ACCESSION   DASSQV010000513 DASSQV010000000
VERSION     DASSQV010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866997"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..462)
                     /locus_tag="VFL04_09005"
     CDS             complement(40..462)
                     /locus_tag="VFL04_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839887.1"
                     /translation="MAALRARPRAVAPEWRRLALGGPILRSLLAISLSFALLGGIQAQ
                     AKPDALKLYLDGKYEDSRRACLDELSADPANLEAYVVISWSLLALERWADAENYALKG
                     YDLRRDARLTEALGEAAYHLGRNEAALRNFQNYVSVVQ"
     gene            complement(443..820)
                     /locus_tag="VFL04_09010"
     CDS             complement(443..820)
                     /locus_tag="VFL04_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET7839888.1"
                     /translation="MTEVQINVVDEEQLDTVLAGDVEFSGEMNFKKPLMIKGRVSGVV
                     RSESELYIDEHAVVEADIAATVVSVKGTVIGNIVARERVELFACASVDGDVSAPQSTM
                     ETGCRFHGACRMTASPLDGSSSS"
     gene            complement(859..1797)
                     /gene="rsmA"
                     /locus_tag="VFL04_09015"
     CDS             complement(859..1797)
                     /gene="rsmA"
                     /locus_tag="VFL04_09015"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET7839889.1"
                     /translation="MPNTEEALAGGPEGRGTLPPDYDAPTSLKSLLEAEGLAMSKRFG
                     QNFLTDRKARTRILEELKAEAGASVWEIGPGIGAMTALALEAGLEVRAFEIDHGFARL
                     LRRCYGERPNFRLIEGDFLRTWRAELEAHGPPDRIFGNLPYSVANAIVCALIEGGLVP
                     PRMVFTMQKEAALRMAAVPGSKDYSAFSVLCTSACKVRLAFDLGAGSFWPAPRVTSSV
                     VSLVPRAKPVAADNRAAFSAFVRAAFSSRRKMLRNNLKANPGVWARKGSTADGAETGT
                     DPIEAALKGLGIRADIRAEALRPEELAAVHEALTRA"
     gene            1848..2159
                     /locus_tag="VFL04_09020"
     CDS             1848..2159
                     /locus_tag="VFL04_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839890.1"
                     /translation="MNRDAGSARTRGGTEAALRITAIRTWYRHSETGAAMAAVGGMRE
                     HRRPAAIPHHMRASTRGAASIEASIPTGWKPPKVKMRIGATAAWAPREAARALEMMGG
                     R"
BASE COUNT          477 a          902 c         1045 g          396 t
ORIGIN      
        1 ctcgcccatg taatagtcgg cgagcccgac ccggccccct tactggacga cgctcacgta
       61 gttctggaaa ttcctgaggg cggcctcgtt gcggccaaga tgataggccg cctcgcccag
      121 ggcctcggtg agccgggcgt cgcggcgcag gtcatagccc ttgagggcgt agttctcggc
      181 atcggcccag cgctcgagcg cgagcaggct ccaggagatg acgacatagg cctcgaggtt
      241 ggcgggatcg gcagaaagct cgtcgaggca ggcccgcctc gagtcctcat acttgccgtc
      301 gaggtagagc ttcagggcat cgggcttggc ctgggcctgg attccgccaa ggagggcgaa
      361 ggagagggag atggcgagga ggcttcggag gatggggccg cccagggcca gacgccgcca
      421 ctcgggggcg acggcccgcg gcctagctcg aagagctgcc atcgaggggg gaggctgtca
      481 tccggcatgc cccgtggaaa cggcagccgg tttccatcgt gctctgcggc gcgctcacgt
      541 caccgtcgac ggaggcgcag gcgaagagct cgacccgctc gcgggcgacg atgttgccga
      601 tcacggtgcc cttcacggag accacggtgg cggcgatgtc ggcctcgacg acggcgtgct
      661 cgtcgatgta gagctcgctc tcggagcgga cgacgccgct cacgcggccc ttgatcatca
      721 ggggcttctt gaagttcatc tcgccggaga actcgacatc ccccgcgagg accgtgtcga
      781 gctgctcttc atcgacgacg ttgatctgga cttcggtcat acggatcgat catacgggat
      841 gaggcgaggc ttggcaagct aggcccgggt gagggcttcg tggaccgcgg cgagctcctc
      901 ggggcgcagg gcctcggccc tgatgtcggc tctgattccg aggcccttga gggcggcctc
      961 gatggggtct gtccccgtct cggccccatc cgccgtcgag cccttcctcg cccatactcc
     1021 ggggttggcc ttgaggttgt tgcggagcat cttcctgcgg gagctgaagg cggccctgac
     1081 aaaagccgag aaggcggccc ggttatccgc cgcgacgggc ttcgcccggg gaacgaggct
     1141 tacgacgctg ctcgtgaccc tgggcgccgg ccagaaggat cctgccccga ggtcgaaggc
     1201 gagccgaacc ttgcaggccg aggtgcacag cacggagaag gccgagtaat ccttggaccc
     1261 gggaaccgcg gccatccgaa gcgccgcctc cttctgcatt gtgaagacca tccggggggg
     1321 gacaaggccc ccctcgataa gggcgcagac gatggcgttc gcgacgctgt agggcaggtt
     1381 gccgaagatt cggtcgggcg gcccatgtgc ctcgagctca gccctccagg tgcggaggaa
     1441 gtccccctcg atgaggcgga agttcggcct ctccccatag cagcggcgca ggaggcgggc
     1501 gaagccgtgg tcgatctcga aggccctgac ctcgaggccg gcctccaggg caagggccgt
     1561 catggcgccg atgccggggc cgatctccca gaccgaggcg ccggcttcgg ccttgagctc
     1621 ctcgagaatt cgggtcctgg ccttcctgtc cgtgaggaag ttctgcccga agcgcttcga
     1681 catcgccagg ccctcggcct ccagaaggct cttgagcgag gtcggggcgt cgtagtcggg
     1741 cggcagggtc cccctcccct ctgggcctcc ggccaaggct tcttcggtgt tcggcatgtg
     1801 ggcgagcata cacgaaggcg gtcaaggcgg ctacggaggc agtggcgatg aaccgtgatg
     1861 ccggctcggc caggacgagg ggaggcaccg aggcggcgtt gcgcatcacc gcgatcagga
     1921 catggtacag gcattccgaa acgggggcgg cgatggccgc ggtcggcggc atgagggagc
     1981 acaggaggcc ggcggccatc ccccaccaca tgagggcgag cacgaggggg gcggcgagca
     2041 ttgaggcgag tatcccgacg ggctggaagc cgccgaaggt gaagatgagg atcggggcca
     2101 cggccgcctg ggccccgagg gaagcggcca gggctttgga gatgatgggc gggaggtagg
     2161 gaccgaggac gaactcgaag cggggcgaga ggagggcgag tcccaggatc gccgcgtacg
     2221 agagcgtgaa gccggggctc cgcgccgcct cgggatccca gggcagggcg aggatgaagg
     2281 ccaaggcgag acagcggatc caggtctggg gcctgtcgac ggcgagggcc agggcggcga
     2341 gccacaccat gagcaccgac ctgagcaagg acggccctgg tccggccacc cacatgaaga
     2401 gggccgcgag gatcgccgcc ccgaaacggg cgcgcagggg cccgagcagg ggcctcagac
     2461 aggcgagggc gagaagggcg agcacggaga gatgctggcc cgaaagcgcg aggatgtggc
     2521 tgcagcccgc gcccctgaag gccgccgcat cctcgaggtc gaggttgtcc tgggccccga
     2581 tgaggagggc ctcgaggagg ccgcccgaat ccccgcccgc ccgcctgagg gccaccctgc
     2641 aggcctcccg tatgaggctg cggcagctct ccatcggtcc gccgccgtcg aagaccagaa
     2701 gctcgcgtgc cagggcgaac actgccgccg agccaggcgg cccgcctagg ccacctaggc
     2761 cacctaggcc acctaggcca cctgggccac ccagccctgt ctgggcgcag ctgaggctca
//
LOCUS       DASSQV010000514         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2110844, whole genome shotgun sequence.
ACCESSION   DASSQV010000514 DASSQV010000000
VERSION     DASSQV010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110844"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="VFL04_09025"
     CDS             complement(<1..1626)
                     /locus_tag="VFL04_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7839891.1"
                     /translation="MDRNKWDAAKIILFPLPFLFLAAYLAGLGFPLPAFLAPTRSSLA
                     IAPGLGDAELKRARGRGEAFAVLGDRLLQLPASPLALRAAALPELPVLGPVLEDLEGG
                     LGQLRKARLVVSRPPSAEELASLKAAARRAGAPLSIELIEAPALPLGALTRRYSATDR
                     RLDFELLLEPGARAYATVEVATEPRPGKAAELLYRQSGSALPPDLVLRLSLDKEKSAA
                     VVASFVDASGARSSRRLQLASSLEEQPRVLVISERRGRESFIERLYPTRRATAAEAAG
                     LDLLSYELVVVDGIPIKAIQGRLLQGLLELSERRTGSILFAADSPEFGTKGENPELEA
                     TLPVTLMPRDLKDLPDLGILVMIDVSGSMFGDKLSLAKVTGLELLHNLKSGDLIGLML
                     FSDVHSWVHEFAPNSSIVAAPELEPITAGGGTDLAPALVEGLDRLSRLAIRDKHAVVI
                     TDGVTKPADFQAIADAAKAKGISISAMGIGEDVNKALLERLALRTGGRYYRVGSADEI
                     PALLFEDRQSVARPAFKQGRIPVLALNGDRVAEI"
     gene            1685..2149
                     /locus_tag="VFL04_09030"
     CDS             1685..2149
                     /locus_tag="VFL04_09030"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET7839892.1"
                     /translation="MEKRIIEVFTDSTRRTFHEMFALEASFLGSREIATGKDHGWDIT
                     GLVGLAGQAQGVVALRLTAAILEALLDGSGIEVSGAEERVQLSAGLIGELTNIVAGSA
                     TSALKGLELTIAPPVVIRGPSHEIGWPAIAPVLGLSFGLPTGQFEVDLCLRW"
     gene            2211..>2416
                     /locus_tag="VFL04_09035"
     CDS             2211..>2416
                     /locus_tag="VFL04_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839893.1"
                     /translation="MDFAARRLSGTDPWLNLALEEACLRRGGEDGALLLIYVNDACLV
                     VGRNQNPWTEVAPDAGLPVLRRVSG"
BASE COUNT          348 a          809 c          898 g          361 t
ORIGIN      
        1 tatctcggcg accctgtccc cgttcagggc gaggacgggg atcctgccct gcttgaaggc
       61 cggtcgggcc acgctctgcc tgtcctcgaa gaggagggcg gggatctcgt cggccgagcc
      121 cacgcggtag tagcgccccc ccgtcctcag ggccaggcgc tcgaggaggg ccttgttcac
      181 gtcctcgccg atccccatcg ccgagatcga gatgcccttg gccttggcgg cgtcggctat
      241 cgcctggaaa tccgcgggct tcgtgacacc gtcggtgatg acgacggcgt gcttgtcccg
      301 gatcgccagc ctggacagcc ggtcgaggcc ctccacgagg gcgggggcca ggtctgtccc
      361 ccctcccgcc gtgatgggct cgagctcggg ggcggccacg atgctcgagt tcggggcgaa
      421 ctcgtggacc catgagtgga cgtcggagaa gagcatgagg ccgatgaggt ccccgctctt
      481 gaggttgtgc aggagctcga gacccgtgac cttcgcgagc gagagcttgt cgccgaacat
      541 cgagcccgag acgtcgatca tgacgaggat gccgaggtcg ggcaggtcct tgaggtcccg
      601 cggcatgagg gttaccggca gggtggcctc gagctcgggg ttctcgccct tggtgccgaa
      661 ctcggggctg tcggcggcga agaggatcga gccggtgcgc cgctcggaga gctcgaggag
      721 gccctggagg aggcggccct ggatggcctt gatcggtatc ccgtcgacga cgacgagctc
      781 gtaggagagg aggtcgaggc ctgccgcctc ggccgcggtg gccctccggg tggggtagag
      841 ccgctcgatg aaggactccc tgcccctgcg ctcgctgatg acgaggaccc ggggctgctc
      901 ctcgaggctc gaggccagct ggaggcggcg cgagctgcgc gcccccgagg cgtcgacgaa
      961 gctggccacg acggcagcgg atttctcctt gtccaggcta agcctcagga cgagatcggg
     1021 cggcagggcc gatccgctct ggcggtagag gagctcggcc gccttgcccg gcctgggctc
     1081 ggtcgcgacc tcgaccgtag cgtaggccct tgccccgggc tcgaggagga gctcgaagtc
     1141 gagccgccgg tcggtggccg agtagcgcct tgtcagggcg ccgaggggga gggccggggc
     1201 ctcgatcagc tcgatggaga ggggggcccc cgccctgcgg gcagccgcct tcagcgaggc
     1261 cagctcttcg gccgagggcg ggcgggagac cacgagccgg gccttgcgga gctggccgag
     1321 cccgccctcg aggtcctcga gcacggggcc gaggacgggg agctcgggca gggctgccgc
     1381 ccggagcgcg agcggggagg cggggagctg gaggagacgg tcgccgagga cggcgaaggc
     1441 ctcgccccgg cccctcgccc gcttcagctc ggcgtcgccg aggccgggcg cgatggccag
     1501 agagctccgc gtcggggcga ggaaggcggg caggggaaag ccgaggcccg cgaggtaggc
     1561 cgcgaggaag aggaagggca ggggaaagag gatgattttg gcggcgtccc acttgttgcg
     1621 atccatcggg gcacctattc tagtaccgag gccgcggcgc cgcaatcaca atcgggggta
     1681 ggtcttggaa aaacgcatca tcgaggtctt caccgactcc acccgcagaa ccttccatga
     1741 gatgttcgca ctcgaggcga gtttcctcgg ctccagggag atcgcgacgg gcaaggacca
     1801 cggctgggac atcaccggcc tcgtggggct ggccggccag gcccaaggcg tcgtggcgct
     1861 caggctcacg gccgccatcc tcgaggccct cctcgacggc tcgggcatcg aggtctcggg
     1921 cgccgaggag agggtccagc tctcggccgg cctcatcggc gagctcacga acatcgtcgc
     1981 cggctcggcg acctcggccc tcaagggcct cgagctcacc atcgccccgc cggtcgtgat
     2041 ccgcggcccg agccacgaga tcggctggcc ggccatcgcg ccggtcctcg gcctctcctt
     2101 cggcctgccc acgggccagt tcgaggtcga cctctgcctc aggtggtagc gggcgccgag
     2161 caacgcgcct tttgcctcgc tcgggcctaa atcagtatct tcctctgcgg atggacttcg
     2221 ccgcgcgccg cctttccggc acagacccct ggctcaacct cgccctcgag gaggcctgtc
     2281 tgcgccgcgg gggggaggac ggcgcccttc tcctgatcta cgtcaacgat gcctgtctcg
     2341 tcgtcggccg caaccagaac ccctggaccg aggtggctcc ggacgcgggc ctccccgtcc
     2401 tcagacgggt ctcggg
//
LOCUS       DASSQV010000515         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1364983, whole genome shotgun sequence.
ACCESSION   DASSQV010000515 DASSQV010000000
VERSION     DASSQV010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364983"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFL04_09040"
     CDS             complement(<1..666)
                     /locus_tag="VFL04_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET7839894.1"
                     /translation="MNASQKVLFIDPATGFYRVKRYKVGDYFGPIDLGVHISGRTMSL
                     NFGVGLFAGSILPGSNRLFFSGFSPSWRSFFVSSMGGAGLVFDNLGLNLVSLVGKAPV
                     PSILYLNRQHGEEIEVELLPVDLNAIWRGSECTETGADGTAEGGSRAARLGAYALMDY
                     ALAHFGPRYEGEYRAFAVGPAAAATDFGAIVSAPVQGGRASPVDTWAGRGGLGSKMLQ
                     EHGI"
     gene            complement(679..1542)
                     /locus_tag="VFL04_09045"
     CDS             complement(679..1542)
                     /locus_tag="VFL04_09045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7839895.1"
                     /translation="MKTFATLPRPEALRPIPCALCGQEGFAPLWDCGAFSFVRCRSCG
                     LVQQNPQPEAPAVAARYDSAYLEYEAENQLVYRDLELLALAELGVLDSLKRRAAQSSG
                     PARLLDVGCATGALLDSFRSIGCEVMGVELCAPAAEYGRSRYRLPIHPGTLESAAFPD
                     ASFDLVHASHLIEHLNDPGAWLDEVGRVLAPDGELVLTTPKVDGFQARLLGSEWRSAI
                     YDHLYLFSLRSLVRLLGTKGFRLERSITWGGWAKGLRPAFLKKPLDALAKRWGLGDVM
                     ALVARKAHPRV"
     gene            complement(1566..>2294)
                     /locus_tag="VFL04_09050"
     CDS             complement(1566..>2294)
                     /locus_tag="VFL04_09050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7839896.1"
                     /translation="IAADDGLVRAISEGELSFTFEEGSHPAGLPTALGSFVVVEHPHD
                     MAAVYSHLAPGSISSYLKTVKSDSILGKTGSSGWTEGPGLLLQIFDRRLDQWVNPLLV
                     LPPTVDTKPPVIKSMALVRGTKTYVLGDTASVPQGSYLITADVADPADSPWTAGPLAP
                     LSIRLAVDGAEMAKEVFDVAEGKDGRLRLFSANPKDFAAYRTKDGRYALGERLLSRGK
                     VVFEVSAEDENGNRRTASWSVMVE"
BASE COUNT          368 a          728 c          859 g          339 t
ORIGIN      
        1 gatgccgtgc tcctggagca tcttggagcc cagtccgccg cggccagccc aggtgtcgac
       61 cgggctcgcc ctccccccct ggacgggggc cgagacgatc gcgccgaaat cggtggcggc
      121 ggccgcgggg ccgacggcga aggcgcggta ctcgccctcg taacgcggcc cgaagtgggc
      181 gagggcgtag tccatgaggg catatgcgcc gagacgggcg gcgcgcgacc cgccctcggc
      241 ggttccgtcg gctcccgtct cggtgcattc ggagccgcgc cagatcgcgt tgaggtcgac
      301 ggggaggagc tcgacctcga tctcctcgcc gtgctggcgg ttcaggtaga ggatcgatgg
      361 gactggggcc ttgcccacga ggctcacgag gttgaggccg aggttgtcga acaccaggcc
      421 agcccccccc atgctcgaca cgaagaagct ccgccagctg ggcgagaagc ccgagaagaa
      481 gaggcggttc gagcccggaa ggatcgagcc ggcgaagagg ccgacgccga agttgaggct
      541 catcgtgcgg ccggagatgt ggacgccaag gtcgatcggc ccgaagtagt ctccgacctt
      601 gtagcgcttg acccggtaga acccagtcgc gggatctatg aacaggacct tctggctggc
      661 gttcatcact ctccctcctc agacgcgcgg gtgggctttc cgcgcgacca gtgccatgac
      721 atccccgagg ccccagcgct tggctagggc atccaggggc ttcttcagga aggcgggcct
      781 gaggcccttg gcccagcctc cccaggttat cgagcgttcg aggcggaacc ccttggtccc
      841 aaggaggcgg acgaggctcc ggagcgaaaa gaggtagagg tggtcgtaga tggcgctcct
      901 ccactcgctg ccgaggagcc gggcctggaa gccgtcgacc ttgggcgtcg tcagcacgag
      961 ctcgccgtcc ggggcgagga cccggcccac ctcgtcgagc caggcgcccg gatcgttcag
     1021 gtgctcgatc aggtgggagg cgtggacgag gtcgaatgag gcgtccggga aggctgcgga
     1081 ctcaagggtg cccgggtgga tggggaggcg gtagcgggag cggccgtact cggccgctgg
     1141 ggcgcagagc tcgaccccca tgacctcgca gccgatggag cggaaggagt cgaggagggc
     1201 acccgtcgcg cagcccacgt cgagcagcct ggccggccct gaggactggg ctgcccttct
     1261 cttgagcgag tcgagcacgc cgagctcggc gagggccaag agctccaggt cgcgatagac
     1321 gagctggttc tcggcctcgt actcgagata ggccgaatcg tagcgcgcgg cgaccgccgg
     1381 ggcctcgggc tgggggttct gctgcacgag gccgcaggag cggcagcgca cgaaggagaa
     1441 ggcgccgcag tcccagagcg gggcgaagcc ctcttgaccg cagagggcgc aggggatcgg
     1501 ccttagggcc tcggggcgcg gcagggtggc gaaggtcttc acgcctgttc cgcctcggcg
     1561 ggtcctcatt cgaccatgac ggaccaggac gcggtccgcc tgttgccgtt ctcatcctcg
     1621 gcgctcacct cgaagaccac cttgccgcgc gagaggaggc gctcgcccag agcgtacctc
     1681 ccgtccttgg tccgataggc agcgaagtcc ttcgggttcg cggagaagag gcggaggcgg
     1741 ccgtccttgc cctcggccac gtcgaagacc tccttcgcca tctcggcccc gtcgactgcg
     1801 agcctgatcg agaggggggc caggggtccg gccgtccagg gagagtcggc ggggtcggcc
     1861 acgtcggcgg tgatcaggta gctgccctgg ggcaccgagg ccgtgtcgcc gaggacgtag
     1921 gtcttcgtgc ccctgaccag ggccatggac ttgatcaccg ggggcttggt gtccacggtg
     1981 ggaggaagga cgaggagggg gttcacccac tggtcgagcc tccggtcgaa tatctgcagg
     2041 aggaggccgg gcccctcggt ccagcccgag ctcccggtct tgccgaggat cgagtcgctc
     2101 ttcacggtct tcaggtagct ggagatcgag cccggggcga ggtgggagta gacggcggcc
     2161 atgtcatggg gatgctcgac gacgacgaag gagcccaagg ccgtcggcag accggctgga
     2221 tggctgccct cctcgaaggt gaaggagagc tccccctcgg agatggcccg cacgaggccg
     2281 tcatcggcgg cgat
//
LOCUS       DASSQV010000516         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_742274, whole genome shotgun sequence.
ACCESSION   DASSQV010000516 DASSQV010000000
VERSION     DASSQV010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742274"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="VFL04_09055"
     CDS             <1..1256
                     /locus_tag="VFL04_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839897.1"
                     /translation="AIVLMREAVSRNRKDGMAWYTLGLAQAGAGKAADAVYSFKTAAD
                     LRPDDEVVRIAMEDAVVDSSSPEATSREAFADWHFKRGKEFEDRSLFDQAIFEYRRGL
                     RLYPYSKKGRVLYANLLQTRGYPGKYLSELQFLKDNGKADQAVLDSIEIYDSLLTDAV
                     GRDWNIDEQALPKRPYRIALMYLDQPSLAIHTALGEVLLRYLKDVLASSGRVSVLPTP
                     SRADSLSEAFRIARGAEADYFALLSGKETDRDLVITAELRVGRTGSPAGSYTAYRTGN
                     DRVKNSSIRIADLLVGSLPIKGKILQRSQDRVLVDLGKSEGLAKGAKLLVIKSGGIRT
                     QAEGIGLSWDPAAQLGSIDLSRAGEEASEGSLKLAGFFDTVNIGDEVLLAPAPAKAGG
                     APPAPVPAPAASPFPGLFMAIRQLR"
     gene            complement(1269..2633)
                     /locus_tag="VFL04_09060"
     CDS             complement(1269..2633)
                     /locus_tag="VFL04_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839898.1"
                     /translation="MDKEPAGLLLALAISLFCLGCTAAGTDPPKNGGIAPAETAQAYV
                     VFAWNDLGMHCLNPSYDTAVILPPYNTVNAQVLKRGDPPELVTSGVTVSYALVDNSYS
                     VGKGQFGQFWTYMMSLFGKSQADNIGLAGKGLSGTMDWVGADGVYRAVGIPVVPRTDS
                     APLVRAPYQQAVITVRDGAGQVIASTKATVPTSDEINCAKCHAPGASIQASFQDILDK
                     HDALSSTNLAAAKPVLCASCHSSPALGTPLQAGIPYLSASIHGFHATRGASCLDCHPG
                     ATTLCNRSLAHTSSDGNCTSCHESMQVVASSIAAGRVPWQTEPACGSCHLSPTQATLT
                     RIPSPGLSSIPEVNTGAALDRTSKGHGGLSCTACHSSPHAMVPSREAKDNFQALAYQG
                     KAVTIGSCGACHPSSQGRGAAEFMDTHGGPNPEEFSACAVCHTAVPSADIQLWPHGFA
                     WKPR"
BASE COUNT          415 a          908 c          908 g          420 t
ORIGIN      
        1 aggccatcgt cctcatgcgc gaggcggtct cccggaaccg caaggacggg atggcctggt
       61 acaccctcgg cctcgcccag gccggggcag ggaaggccgc cgacgccgtg tacagcttca
      121 agaccgccgc cgacctcagg cccgacgacg aggtcgtgcg catcgccatg gaggacgccg
      181 tcgtcgactc ctcgagcccc gaggcgacat ccagggaggc cttcgccgac tggcatttca
      241 agcgcggcaa ggagttcgag gaccgcagcc tcttcgacca ggccatcttc gagtaccgcc
      301 gcggcctcag gctctacccc tactccaaga aggggagggt cctctacgca aacctcctcc
      361 agaccagggg ctatcccggc aaatacctct ccgagctcca gttcctcaag gacaacggca
      421 aggccgacca ggcggtcctc gactcgatcg agatctacga ctccctcctc accgacgccg
      481 tgggcaggga ctggaacatc gacgagcagg cccttcccaa acggccctac cgcatcgccc
      541 tcatgtacct ggaccagccg agcctggcca tccacacggc cctgggcgag gtcctcctcc
      601 gctacctgaa ggacgtcctc gcctcctcgg gccgggtttc ggtcctcccg accccaagcc
      661 gggccgacag cctctccgag gccttccgca tcgcgcgcgg cgccgaggcc gattacttcg
      721 ccctcctctc cggcaaggag accgaccgcg acctcgtgat cacggccgag ctccgcgtgg
      781 gccggacggg gagcccggcg ggctcctata ccgcctacag gacgggcaac gacagggtca
      841 agaactcctc gatccgcatc gccgacctcc tggtgggctc cctgcccatc aagggcaaga
      901 tcctccagcg gagccaggat cgggtcctcg tcgaccttgg caaaagcgaa ggcctcgcca
      961 agggcgccaa gctcctcgtg atcaagagcg ggggcatcag gacccaggcc gagggcatcg
     1021 gcctgagctg ggacccagcc gcccagctcg gcagcatcga cctttcccgg gccggggagg
     1081 aggcgagcga gggcagcctg aagctcgccg gcttcttcga caccgtcaac atcggggacg
     1141 aggtcctcct cgcccccgcc ccggcgaagg ccggcggggc ccctccggcc cccgtccctg
     1201 cccccgccgc ctcccccttc cccggcctct tcatggccat ccgccagctc agatagggga
     1261 tctcccgctc agcgcggttt ccaggcgaag ccgtgcggcc agagctggat gtcagccgag
     1321 gggacggcgg tgtggcagac cgcgcatgcc gagaattcct cagggttggg tccgccgtgc
     1381 gtgtccatga actcggcggc gccccttccc tgggagctcg gatggcaggc tccgcagctc
     1441 ccgatcgtga cggccttgcc ctggtaggcg agggcctgga agttgtcctt ggcctcgcgg
     1501 ctcgggacca tggcgtgggg gctgctgtgg caggcggtgc agctgaggcc tccatggccc
     1561 ttcgaggtgc ggtcgagcgc ggcgccggtg ttgacttccg gaatcgaact gagccccggg
     1621 gaagggatcc tcgtcagggt cgcctgggtc ggggacaggt ggcaggagcc gcaggccggc
     1681 tcggtctgcc aaggcacgcg gccggcggcg atgctgctgg cgactacctg catcgattcg
     1741 tggcagctcg tgcagttccc gtcggagctc gtgtgcgcga ggctgcggtt gcacagggtg
     1801 gtcgcgccgg gatggcagtc gagacagctc gcgcccctcg tggcgtggaa accgtggatc
     1861 gaggccgaga ggtagggtat gccggcctgc agcggggtgc cgagggctgg gctcgagtgg
     1921 caggaggcgc agaggacggg ctttgcggca gcgaggttcg tggaggacag ggcatcgtgt
     1981 ttgtcgagga tgtcctggaa actcgcctgg atcgaggccc cgggggcatg gcatttggcg
     2041 cagttgatct cgtccgaggt gggcacggtc gccttggtgc tcgcgatgac ctggccggct
     2101 ccgtccctga cggtgatcac cgcctgctgg tagggggcgc ggaccagggg ggcggagtcc
     2161 gtccgaggaa cgacgggtat tccgactgcc cggtagaccc cgtcggcccc gacccaatcc
     2221 atcgtcccgc tgaggccctt accggcgagc ccgatgttgt cggcctggct cttgccgaag
     2281 aggctcatca tgtaggtcca gaactgcccg aactggccct tgcccaccga gtagctgttg
     2341 tcgaccaggg cataggagac cgtcactccg cttgtcacca gctcgggggg atccccccgc
     2401 ttgagcacct gggcgttgac cgtgttgtag ggcgggagga tgacggccgt gtcgtagctc
     2461 ggattcagac agtgcatgcc gaggtcgttc caggcgaaga ccacgtaggc ctgcgcggtt
     2521 tccgccggcg cgatcccgcc gttctttggc gggtcagttc cggcggcggt gcagccgagg
     2581 caaaacaggg agatggccag agcgaggaga aggccggcag gttccttgtc catatccaga
     2641 ccccccttcc c
//
LOCUS       DASSQV010000517         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_2235491, whole genome shotgun sequence.
ACCESSION   DASSQV010000517 DASSQV010000000
VERSION     DASSQV010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2235491"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..165
                     /locus_tag="VFL04_09065"
     CDS             37..165
                     /locus_tag="VFL04_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839899.1"
                     /translation="MDVVGGQLKAGLALVGFLEDISGWGFLDPHIRTFIATCAVKP"
     gene            174..854
                     /locus_tag="VFL04_09070"
     CDS             174..854
                     /locus_tag="VFL04_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7839900.1"
                     /translation="MSPRLFRALPLLLILALALGCKGKPTQQAQVEGAPNTLSSASSV
                     PAPTGTQHYPARSRAELPPVPQPAMPATIPGYTELDPETGLHMTGGPTLIQLASWRLK
                     VGGKVRFPLELDYDSLRMLPGIRTRATIICRGYFEDTAVWAGASLASILEAASPLSSA
                     KGLSLIGADGYSASVTMEQAKTGDNFIAYELAGRPVTVLQGFPARAAFPSLLGNKWTK
                     WLLEIRVE"
     gene            970..1233
                     /locus_tag="VFL04_09075"
     CDS             970..1233
                     /locus_tag="VFL04_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839901.1"
                     /translation="MLTLGPGEREGLQASVAQMLDFFSIMDGLELGAIGTSQHCDPAS
                     EPQRADEVRDFNNRNPSNNPLSNTLVNNAPETSGRSVVIPNVL"
     gene            1250..>2102
                     /locus_tag="VFL04_09080"
     CDS             1250..>2102
                     /locus_tag="VFL04_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET7839902.1"
                     /translation="MSHLPQWGGVLEGPRLDAYAQLLEEREREIGAFLELVPRQPARA
                     LGADGLPKGGPLSGLPFAVKDNIAVEGQILSCGSRILEGFRSPYSATCVRRLLEAGAV
                     CIGKTNLDEFGMGSSTENSAYKATVNPWDRDRVPGGSSGGSAAAVAAGMVPFALGTDT
                     GGSVRQPAAFCGLYGLKPSYGAVSRFGLVAYASSLETAGVIARSVDLVETVFGVMAGG
                     DPKDRTSVDAPKPRAEPAMSGPASAGASSLAGRRIGVPRGSAEGLDPGIARGFASALE
                     ALRDAGAL"
BASE COUNT          304 a          783 c          688 g          327 t
ORIGIN      
        1 taccggccgg gcggcctcgt cggctaggag gaagccatgg atgtcgtcgg cggccagctt
       61 aaggccggct tggccctcgt gggcttcctc gaggacatct caggctgggg cttcctcgat
      121 ccccacatac ggaccttcat cgccacctgc gcggtgaagc cataggaatc ccgatgtccc
      181 cgcggctgtt tcgcgcattg cccctcctcc tcatcctggc cctcgccctc ggctgcaagg
      241 ggaaacccac gcagcaggcc caggtcgagg gcgccccgaa cacgctgagc tcggcctcct
      301 cggtgcccgc gccgacgggg acccagcatt atcccgcccg cagccgcgcc gagctgccgc
      361 ccgtccccca gcccgcgatg cccgccacca tccccggcta caccgagctc gaccccgaga
      421 ccggcctcca catgacgggg ggaccgaccc tgatccagct cgcctcgtgg cggctcaagg
      481 tggggggcaa ggtgcgcttc cccctcgagc tcgactacga ctcgctgcgc atgctccccg
      541 ggatcaggac aagggcgacg atcatctgca ggggctattt cgaggacacc gcggtctggg
      601 ccggggcctc gctggcctcg atcctcgagg ctgcctcgcc cctgagctcg gcgaagggcc
      661 tgagcctcat cggggccgac ggctactcgg cctcggtgac gatggagcag gcgaagacgg
      721 gcgacaattt catcgcctac gagctcgccg gcaggccggt gaccgtcctc cagggtttcc
      781 cggcccgcgc cgccttcccc tcacttctcg gcaacaagtg gacgaagtgg ctcctcgaga
      841 tccgggtcga gtgagcccgg ccggatgacg cgcggggccg cttgacgccg cggcttccgg
      901 cgtgggaaag tagcgcgggg gtacgcttct atgcaaaagg acgacctcga tctgaccgct
      961 gagctggcca tgctgactct cggtcccggg gagcgcgagg gcctgcaagc ctcggtcgcg
     1021 cagatgctcg atttcttctc catcatggac ggcctcgagc tcggggccat cgggaccagc
     1081 cagcactgcg acccggcctc cgagccccag cgcgccgacg aagtgcgcga tttcaacaac
     1141 cgcaaccctt ccaacaatcc cctctcgaat accctcgtca ataacgcccc ggagacctcg
     1201 gggcggtccg tcgtaattcc gaacgtcctg taagacccgg aggtctccaa tgtctcatct
     1261 cccgcaatgg ggcggggtcc tcgaaggacc tcggctcgac gcatatgcac agctgctcga
     1321 ggagcgcgaa cgcgagatcg gcgccttcct ggagctcgtt ccgcgccagc ccgcccgggc
     1381 cctcggcgcc gacggccttc caaagggggg gcccctgtcg ggcctgccct tcgccgtcaa
     1441 ggacaacatc gccgtcgagg gccagatcct gagctgcggc tcgcgcatcc tcgagggctt
     1501 ccgctccccc tactccgcga cctgcgtccg ccgcctcctt gaggcggggg ccgtctgcat
     1561 cggcaagacc aacctcgacg agttcggcat gggctcctcc accgagaact ccgcctacaa
     1621 ggccaccgtg aacccctggg accgggaccg ggtgcccggg ggctcgtcgg ggggctcggc
     1681 ggctgccgta gcggcgggga tggtgccctt cgccctcggg acggacaccg gaggctcggt
     1741 ccgccagccc gcggccttct gcgggcttta cggcctcaag ccgagctatg gggccgtctc
     1801 gcgcttcggc ctcgtcgcct acgcctcgtc cctggagacg gccggcgtca tcgcgcgctc
     1861 ggtcgacctc gtcgagaccg tcttcggggt gatggccgga ggggacccaa aggacaggac
     1921 cagcgtcgat gcgccgaagc cccgggccga gcccgccatg tctgggcccg catcagctgg
     1981 cgcctcgtcc ctcgcaggcc ggcggatcgg cgtcccgcga ggctcggcag aggggctcga
     2041 ccctggaatt gcccggggct tcgcttcggc cctcgaggcc ctgcgcgatg ccggggccct
     2101 gg
//
LOCUS       DASSQV010000518         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_369820, whole genome shotgun sequence.
ACCESSION   DASSQV010000518 DASSQV010000000
VERSION     DASSQV010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369820"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VFL04_09085"
     CDS             complement(<1..1149)
                     /locus_tag="VFL04_09085"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET7839903.1"
                     /translation="MPDTGATEQTSVTMEKIVSLAKRRGFVFQSSEIYGGQSGAWDYG
                     PMGVELKNRIARFWWKEMTQLHDDIVGLDAAILMHPRTWEASGHVENFTDPLVDCKKC
                     KQRFRADMIPEDKLARKECPECGGELTETRKFNLMFKTNIGPVDDGSGLIYLRPETAQ
                     GIYVNYKNIVQSNRMKVPFGIAQVGKAFRNEIVTKNFIFRTCEFEQMEMQYFVRPGDD
                     EKYFEYWRDQRWKYYEKLGIRMDRIRWHRHGPGELAHYAKDAYDIEYEFPMGWKELEG
                     VHNRTDFDLKRHQEYSGKDLQYIDQDNNNERYIPFIVETSAGLTRQLLMILCDAYDEQ
                     KVADKGNDDDWRTVMRFHPTLAPVTVAVLPLMKKDGLAELAQDIRQGLK"
     gene            complement(1350..1724)
                     /locus_tag="VFL04_09090"
     CDS             complement(1350..1724)
                     /locus_tag="VFL04_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7839904.1"
                     /translation="MDDSEAGELCPKVEAAFALLARKWTGLIIRALIPGALYFCELEK
                     AIPALSARVLALRMRELEEAGVVSRTVGTASPVRVSYSLTEKGRGLEPVVAGIALWAE
                     AWSQVPEGEKTLVHADSAGYNP"
     gene            1820..>2149
                     /locus_tag="VFL04_09095"
     CDS             1820..>2149
                     /locus_tag="VFL04_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET7839905.1"
                     /translation="MEGSASSSLATRTWATPASEESIARTVAALAANGIQTWVVDSTA
                     EAAAKTLELVPPGSEVMTATSITLDETGIARELNESGSFDAVKPRLSAMDRETDERAM
                     RKLGASPD"
BASE COUNT          342 a          685 c          687 g          435 t
ORIGIN      
        1 cttgaggccc tgtctgatgt cctgggccag ctcggcaagg ccatccttct tcatgagggg
       61 aaggacggcc acggtgaccg gcgcgagggt cggatggaag cgcatcacgg tccgccagtc
      121 gtcgtcgttg cccttgtcgg cgaccttctg ctcatcgtag gcgtcgcaga ggatcatgag
      181 gagctgcctg gtcaggccgg ccgaagtctc gacgatgaag gggatgtagc gctcgttgtt
      241 gttgtcctgg tcgatgtact ggagatcctt gcccgagtac tcctgatggc gcttgaggtc
      301 gaagtcggtc cggttatgga ccccctccag ctccttccag cccatgggga actcgtactc
      361 gatgtcgtag gcgtccttcg cgtagtgggc cagctcgccg gggccatggc ggtgccagcg
      421 tatcctgtcc atgcggatgc cgagcttctc gtagtacttc cacctctggt cgcgccagta
      481 ctcgaagtac ttctcgtcgt ccccgggcct gacgaagtac tgcatctcca tttgctcgaa
      541 ctcgcaggtc cggaatatga agttcttcgt cacgatctcg ttgcggaagg ccttccccac
      601 ctgggctatc ccgaagggga ccttcatgcg gttggactgg acgatgttct tgtagttgac
      661 gtagatgccc tgggcggtct cgggccgcag gtagatcagg ccggagccgt cgtcgaccgg
      721 cccgatgttg gtcttgaaca tgaggttgaa cttgcgcgtc tcggtcagct cgcctccgca
      781 ctccgggcat tccttgcgcg cgagcttgtc ctcggggatc atgtcggccc ggaaacgctg
      841 tttgcatttc ttgcagtcga cgaggggatc cgtgaaattc tcgacgtggc ccgatgcctc
      901 ccaggtcctg ggatgcatga ggatggcggc gtccaggccc acgatgtcgt cgtggagctg
      961 ggtcatctcc ttccaccaga acctggcgat ccggttcttg agctcgacgc ccatgggccc
     1021 gtagtcccag gcgcccgatt gcccgccata gatctccgat gactggaaga cgaaaccccg
     1081 ccttttggcg aggctgacga tcttctccat tgtgacgctg gtctgttcgg tcgcgccggt
     1141 gtcgggcatg ctgcttttcc tttttcgcgt ctctcgtggc ggctttgatg cgccatccgc
     1201 ttcgcgggag ccctgatttc gccccgccga tggttcgcgg cggcgcgccg aggtcggcat
     1261 ggcccccagc ccggctcaag gggcggaggg cgccgcttcg gtgccccgac ggcctgggtc
     1321 cttctggcga ccggagcccg ggtcggggtt cagggattat agccggcgga gtcggcgtgg
     1381 actagggtct tctccccctc ggggacctgg ctccaggcct cagcccagag cgcgatgccc
     1441 gccacgacag gctcgagccc gcggcccttc tcggtgaggc tgtagctcac cctgacgggg
     1501 gaggccgtgc ccacggtccg cgagacgacc ccggcctcct cgagctcgcg catcctgaga
     1561 gcgagcaccc tcgcgctcag ggcggggatg gccttctcga gctcgcagaa gtaaagggct
     1621 ccggggatga gggcgcgaat tatcaggccc gtccacttcc tggcgaggag ggcgaaggcc
     1681 gcctcgacct tcggacagag ctcgcccgct tccgaatcgt ccatgacaat agtataccac
     1741 aggagcttga cctttgacca tcaagattac tagcttacgt tatgttactt actaacgaat
     1801 gcaaagtgga ggtttcgaca tggaaggatc tgcatcgtca tccctagcca cccggacttg
     1861 ggccaccccg gcctcggagg aatccatagc gcgcacggtc gcggccctgg ccgccaacgg
     1921 gatccagaca tgggtcgtgg acagcaccgc cgaggccgcc gccaagaccc tcgagcttgt
     1981 gcccccgggc tccgaggtca tgaccgccac atcgatcacc ctcgatgaga cgggcatcgc
     2041 aagggagctg aacgagtcgg gatccttcga tgccgtaaag ccccggctct cggcgatgga
     2101 cagggaaacc gacgagcggg ccatgcgcaa gctcggcgcc tcccccgac
//
LOCUS       DASSQV010000519         4592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1113916, whole genome shotgun sequence.
ACCESSION   DASSQV010000519 DASSQV010000000
VERSION     DASSQV010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113916"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VFL04_09100"
     CDS             <1..882
                     /locus_tag="VFL04_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET7839906.1"
                     /translation="GLEALERGVGSNRALLPWLESLLPRASRERLAALAALAPASGGA
                     LPDAPTPVPGPVQTIAGLRFRALRGGSFQVSGESPEGRAVPYLVTLPPYSLAETEVTR
                     RQWASFLAAVPAWRPENRERLVAEGLADESYLADWGTERPDEPVTGVSWEAARAYCAW
                     LSLSAPAGREVRLPSEAMWEHAAGVGGDAKKPDGVFSRPDRRGPEAAASRDALGLYDL
                     FGNVWEWCSDAYVPYPALLPEGSPWIGSERSVRGGGWADRAETVDLGSRGSFPPSFSS
                     PFLGFRPAMVDLRTGRR"
     gene            879..1346
                     /gene="smpB"
                     /locus_tag="VFL04_09105"
     CDS             879..1346
                     /gene="smpB"
                     /locus_tag="VFL04_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET7839907.1"
                     /translation="MSIPDGVKVVALNRRARFDYAVDETFECGIVLEGTEVKSVKDNR
                     ISFPDAFAEVRDGAVWLRNFHISQYAFSSLFNHDPDRLKKLLLHAQEIKRIDRRVREK
                     GYTLVPLSVYLKHGLVKIELGLAKGKKQYDKRSDIKERELDREMRRGLRRRNE"
     gene            1349..1891
                     /locus_tag="VFL04_09110"
     CDS             1349..1891
                     /locus_tag="VFL04_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HET7839908.1"
                     /translation="MRITGGKYLGRTIEVAKGGLEIRPAMDRMRESIFAVLGDLSGLS
                     FLDLFSGSGVIALEAASRGASPVVCVEKDRAKFPMLLRNASISEERIECKSTSSELFL
                     ARNKQAFDLVFCDPPFPYRYRAELLASLSERPTSAPGGLVLIHYPMEDRLLDRYGSLG
                     LEDERVYGRSHVRIYRKDGV"
     gene            complement(1895..2074)
                     /locus_tag="VFL04_09115"
     CDS             complement(1895..2074)
                     /locus_tag="VFL04_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839909.1"
                     /translation="MDTADKMPALAAQTGSAARPEPRLPTSPAAEWLVPSPRARRRPE
                     SPPERLAAYLKALDL"
     gene            2208..2549
                     /locus_tag="VFL04_09120"
     CDS             2208..2549
                     /locus_tag="VFL04_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839910.1"
                     /translation="MDKNLFQRFPGEGLIKTADEPISGVGSDRKVKLNRRGNELFNSG
                     DVETARRIFQTTGYSDGLIRVGDRYLGEGRPVDALKMYWLAKDTRKTTAMIDSIVIVI
                     EKLLREEEEET"
     gene            2546..3673
                     /locus_tag="VFL04_09125"
     CDS             2546..3673
                     /locus_tag="VFL04_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7839911.1"
                     /translation="MSDHPNQDQSHREAQSPELAEIAELSKKGYQYLKENRVEEAEEC
                     FARILERDRENNYALVGLGDAARKRGAFRDAVDHYKKCLVYHPGNNYALFGLADCYKA
                     LNQYQKAIEIWEQYLLHDNKNITVLTRVADAYRKVRDFRKSKAIYLRVLEMEADNPYA
                     LIGLGHLHYDFKEYKAALGYWQRMVELQGDEVDIRVLTSIGNCYRKLKQFELGVPYFD
                     KALVKEPDNFYALFGLADCYRGTGQQHKSLDYWNLILAKDPRNKVILTRAGDAYRSME
                     EFEKAADYYERALNIEFDVYAVLGLAVIARLQGKHQDAIVSLRKLIQNDPKNYRLYLE
                     LAQCYQATGEKQRALETLADFQRLGIRNIYIQDAVAKLQRL"
     gene            3670..4569
                     /gene="rlmN"
                     /locus_tag="VFL04_09130"
     CDS             3670..4569
                     /gene="rlmN"
                     /locus_tag="VFL04_09130"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET7839912.1"
                     /translation="MSDSLYGMSPAQIAEACAFRQAFRARQVYRWLARGVSSFEEMTD
                     LPLAERERLSRLIPRIRSSQVAAALPDEDGSLKLQIRLADGAAVECVLLEDLEGRKTA
                     CLSSQVGCPMRCAFCKTGTLGFLRDLKPGEIMEQFHFLAERRGEISNVVFMGMGEPML
                     NLASVRSAITILSDPAGPGISLRKITISTSGIVPGILDLAEHGPMVRLAVSIAAADDG
                     LRSELMPVNRSHDLAALKAALLRYQETTGERITLELPIMGNSNASVADAQALAAWTTP
                     LRVQVNVIPWNRVPGLHFVEPTM"
BASE COUNT          824 a         1608 c         1455 g          705 t
ORIGIN      
        1 gggctcgagg ccctcgagcg cggcgtcggc tcgaatcggg ccctcctgcc ctggctcgag
       61 tccctcctgc ccagggcttc gcgcgagcgc ctcgcggccc tcgccgccct cgctcccgcg
      121 agcggcgggg cgcttccgga cgcccccacc cccgtgccgg gccccgtcca gaccatcgcg
      181 gggctccgct tcagggccct gcggggaggc agcttccagg tcagcggcga atcgcccgag
      241 gggcgggccg tcccctacct ggtcaccctc cctccctact ccctcgccga gaccgaggtc
      301 acgagacggc agtgggcctc cttcctcgcc gccgtcccgg cctggaggcc tgagaacagg
      361 gagaggcttg tggcggaggg cctggccgat gagtcatacc tcgcggactg ggggaccgaa
      421 cgtcccgacg agccggtgac aggggtgtcc tgggaggccg cacgggccta ctgcgcctgg
      481 ctctcgctct cggcgccggc gggcagggag gtcaggcttc cgagcgaggc aatgtgggaa
      541 cacgccgcgg gcgtcggggg cgacgcgaag aagccggacg gggtattctc ccgaccggac
      601 aggaggggac cggaggcggc cgcgagccgc gacgccctcg gcctctacga cctcttcggg
      661 aatgtctggg agtggtgttc ggacgcctat gtcccctacc ccgcactcct tcccgagggg
      721 agcccatgga tcgggagcga gcgctcggtc cggggcggtg gctgggcgga ccgagccgag
      781 accgtggacc tgggaagcag gggttccttt cccccctcct tctccagccc cttcctcggc
      841 ttcaggcccg ccatggtgga cctaaggaca ggcagaagat gagcattccc gatggcgtga
      901 aggtggtggc cctgaaccgc cgcgcccgat tcgactacgc ggttgacgag accttcgagt
      961 gcggcatcgt cctcgagggc accgaggtga agtccgtcaa ggacaaccgc atctccttcc
     1021 ccgacgcctt cgccgaggtc agggacgggg cggtgtggct ccgcaacttc cacatctcgc
     1081 agtacgcatt ctcgtcgctt ttcaaccacg atccggaccg cctgaagaag ctcctcctgc
     1141 acgcgcagga gatcaagcgc atcgacagga gggtcaggga gaagggctac accctcgtcc
     1201 ccctctcggt gtacctgaag cacggcctcg tgaagatcga gctcggcctg gccaagggca
     1261 agaagcagta cgacaagcgc tccgacatca aggagcggga gctcgaccgc gagatgcggc
     1321 gcggtctgcg gcgccggaac gagtagccat gcggataacc ggagggaagt acctgggcag
     1381 gaccatcgag gtggccaagg gcggcctcga gatcaggccg gccatggaca ggatgcgcga
     1441 atccatcttc gccgtcctcg gcgacctctc gggcctgtcc ttcctcgacc tcttctcggg
     1501 ctcgggggtc atagccctcg aggccgcgag caggggagcc tcgcccgtcg tctgcgtcga
     1561 gaaggacagg gccaagttcc cgatgctcct caggaacgcc tccatatccg aggagaggat
     1621 cgagtgcaaa tcgacctcgt ccgagctctt cctcgcgcgg aacaagcagg ccttcgacct
     1681 ggtcttctgc gaccccccct tcccctaccg ctaccgggcc gagctcctgg cgagcctctc
     1741 ggagaggccg acctcggccc ccggaggcct ggtcctcatc cattacccga tggaggaccg
     1801 cctcctcgac cgctacggct cgctcggcct cgaggacgag cgcgtgtatg ggcggtccca
     1861 cgtgaggatc tacaggaagg acggggtcta gccgctagag gtcgagggcc ttgaggtagg
     1921 cggcgaggcg ctccggcgga ctctcgggcc gccgccgggc gcggggcgaa gggacaagcc
     1981 actccgccgc gggggaggtc gggagccggg gctcgggtcg ggccgcgctc ccggtctgcg
     2041 cggccagcgc gggcatcttg tctgctgtat ccatggagcc tccgaagcct cgttcctggg
     2101 acaaggatat cgcgggaccg gagcgaaaac agcctgccca ctgacggatc tgaaggttga
     2161 ttatttctcc aatatctcgt atagttatcg agtacggcca gttatccatg gataaaaacc
     2221 tgttccagcg ctttcccggc gaagggctga tcaagacggc cgacgagccg atctccggcg
     2281 tcggatcaga ccgcaaggtc aagctcaacc gcaggggcaa tgagcttttc aacagcgggg
     2341 atgtcgagac cgcgaggcgg atattccaga ctaccggata ttcggatggg ctcatccgcg
     2401 tgggggaccg gtatctcggg gaggggcgac ctgtggacgc cctcaagatg tactggctcg
     2461 ccaaggacac gcggaagacg acggccatga tcgattcgat cgtcatcgtg attgaaaaac
     2521 tccttcggga agaagaggaa gaaacgtgag cgaccatccg aaccaggacc aatcacatcg
     2581 cgaggcccag agccccgagc tcgccgagat cgccgagctc tcgaagaagg gttaccaata
     2641 tctcaaggag aaccgcgtcg aggaggcgga ggagtgcttc gcgcggatcc tcgagcgcga
     2701 ccgcgagaac aactacgccc tggtcgggct cggcgacgcc gcccgcaagc gcggcgcctt
     2761 ccgcgacgcc gttgaccact acaagaaatg cctcgtctac cacccgggca acaactatgc
     2821 gctcttcggc ctcgccgact gctacaaggc cctgaaccag taccagaagg cgatcgagat
     2881 ctgggagcag tacctgctcc acgacaacaa gaacatcacg gtcctcaccc gggtcgccga
     2941 cgcctaccgc aaggtccgcg acttccgcaa gtccaaggcc atctacctga gggtcctgga
     3001 gatggaggcc gacaacccct acgccctcat cggcctcggc cacctgcact acgacttcaa
     3061 ggagtacaag gccgccctcg gctactggca gaggatggtc gagctccagg gcgacgaggt
     3121 cgacatcagg gtcctgacct cgatcggcaa ctgctaccga aagctcaagc agttcgagct
     3181 cggcgtcccc tacttcgaca aggccctcgt gaaggagccg gacaacttct acgcgctctt
     3241 cggccttgcc gactgctacc gcggcaccgg ccagcagcac aagtccctcg actactggaa
     3301 cctcatcctc gcgaaggacc cgcgcaacaa ggtcatcctg acccgcgccg gcgacgccta
     3361 ccggagcatg gaggaattcg agaaggcggc ggactactac gagcgggccc tcaacatcga
     3421 gttcgacgtc tacgccgtcc tcggcctcgc ggtcatcgcc cggctccagg gcaagcacca
     3481 ggacgcgatc gtctcgctca ggaagctcat ccagaacgac cccaagaact accggctcta
     3541 cctcgaactc gcgcagtgct accaggcgac gggcgagaag cagcgcgccc tcgagaccct
     3601 cgccgatttc cagcgcctcg gcatccgcaa catctacatc caggacgccg tggcgaagct
     3661 gcagcggctt tgagcgattc cctctacggc atgagcccgg cccaaatcgc cgaggcctgc
     3721 gccttccgcc aggccttccg ggcccgccag gtctaccgct ggctggcccg cggcgtctcc
     3781 tccttcgagg agatgacgga cctgcccctg gccgagcgcg agcggctctc gcgcctcatc
     3841 ccgcggattc gctcctccca ggtggccgcc gcactccccg acgaggacgg ctccctcaag
     3901 ctccagatca ggctcgccga cggagcggct gtggagtgcg tcctcctcga ggacctcgag
     3961 ggacggaaga ccgcctgcct ctcgagccag gtcggctgcc ccatgcgctg cgccttctgc
     4021 aagaccggca ccctcggttt cctgcgagac ctcaagcctg gggagatcat ggagcagttc
     4081 cacttcctgg ccgagaggcg gggggagatc tccaacgtcg tgttcatggg catgggcgag
     4141 cccatgctca acctcgcctc ggtgcgctcc gccataacga tcctcagcga tcccgccggc
     4201 cccggcatct ccctccgcaa gatcacgatc tccacgagcg gcatcgtgcc cggcatcctc
     4261 gacctcgccg agcacggccc catggtgagg ctcgccgtct cgatcgccgc ggccgacgac
     4321 ggcctccgct ccgagctcat gcccgtcaac aggagccacg acctcgccgc gctcaaggcc
     4381 gccctcctcc gataccagga gacgacgggc gagcgcataa cgctcgagct ccccatcatg
     4441 ggcaacagca acgcctccgt cgccgacgcc caggccctcg ctgcctggac aacgccgctc
     4501 agggtccagg tcaacgtcat accctggaac agggtgccgg gcctccactt cgtcgagccg
     4561 acgatgtagc aggtcgaggc ctacatcgac ct
//
LOCUS       DASSQV010000520         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_993297, whole genome shotgun sequence.
ACCESSION   DASSQV010000520 DASSQV010000000
VERSION     DASSQV010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993297"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VFL04_09135"
     CDS             complement(<1..1188)
                     /locus_tag="VFL04_09135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET7839913.1"
                     /translation="MAENLYMIALSPTMSEGTIARWRVAEGASFTAGAVLCEVETDKA
                     TMDYEAPKSGVLLRIVLAAGSSAEVGATIAVIGKEGEDPGSVKAAPQAKGAAVPSGSA
                     PGPQPRSAPGPQSLDSAPGPGPAAQDPSLPPGKAPPSSPLARAIAAARGIDLARVRGS
                     GPGGRVVRRDLEGLAAGPAQTAGGAQAQTGSAAAMAPGPATPQTPAAAYLGRAGQGEQ
                     RIPVSGKRAVIAKRLSASFFGAPHYYLKRRVEAERLLELRASINASREKPLSLNAFIL
                     KLCAEAILRHPYVNSTWAGEAIIQRSSVDLGLAVALPDGLITPVVRDCQTKGIAAIDE
                     ELSALIAKAKTKGLSPEEYEGAGFTVSNLGSYGVEEFTAIINPPGSAILAVGAVMREP
                     VVLP"
     gene            complement(1194..2177)
                     /locus_tag="VFL04_09140"
     CDS             complement(1194..2177)
                     /locus_tag="VFL04_09140"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HET7839914.1"
                     /translation="MSYREALNRALDEEMARDGRVFLIGEEVGEYDGAYKVSKGLLAK
                     YGPDRVRDTPIAELGFTGVGVGAAIAGLRPVVEWMTHNFALLALDQVVNEAAKMRYMS
                     GGRLSVPIVFRGPNGPAEYLAGQHSQALASYWAHVPGLKVIAPSTPSDAYGLLKTAIR
                     DEDPVVVLESELMYAWKGEVAEEEFLVPIGQAALRRRGGDLSIVSFSKPMKAVLEAAE
                     LLAAEGIEAEVIDLRSLRPIDEGTVYESVSRTSRCVVVDESWPQASVGSHLAHLVSRN
                     CFDRLDAPVELVSGEDVPMPYNHALELAAQPDAAKVVAAARRVLYLDPVGA"
     gene            complement(2186..>3108)
                     /locus_tag="VFL04_09145"
     CDS             complement(2186..>3108)
                     /locus_tag="VFL04_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7839915.1"
                     /translation="QEAVAVGAISVLDMAKDYVLTAYRDHGHAIACGMEPKAIMAELF
                     GKSGGVSKGKGGSMHLFDPERRMLGGNGIVGAQVPIATGVALAQSYRRDGGATLCFLG
                     DGAFRQGAIHESLNMARIWNLPIVYIIENNQWGMGTSWKKVSALHDLAATAASYDMPG
                     EACDGMDILDVRRVVARAVERARKEAVPSLVEALTYRYKGHSMSDPQKYRTREDIDEY
                     RKRDPIALLKGRLEEAGLLAEADWSALVAEAERIVEEAVAFAEESPEPPPSELFTDVL
                     LGSSVTDVLWEAGASPAGRAVPGSSGEGRP"
BASE COUNT          412 a         1055 c         1165 g          476 t
ORIGIN      
        1 gggcaggacg acgggctcgc gcatgacagc ccccaccgcc aggatggccg agccgggcgg
       61 gttgatgatg gcggtgaact cctcgacccc gtagctgccg aggttgctga cggtgaagcc
      121 tgccccctcg tactcctcgg gcgagaggcc cttcgtcttg gccttggcga tcagggccga
      181 gagctcctcg tcgatggcgg cgatgccctt ggtctggcag tccctgacga cgggggtgat
      241 gaggccgtcg ggcagggcca cggccaggcc gaggtccacg ctcgagcgct ggatgatggc
      301 ctcgcccgcc caggtggagt tgacgtaggg gtggcggagg atggcctcgg cgcagagctt
      361 gaggatgaag gcgttgagcg aaagcggttt ctccctcgag gcgttgatcg aggcgcgcag
      421 ctcgaggagg cgctcggcct cgacccggcg cttgaggtag taatgggggg cgccgaagaa
      481 ggaggccgag agccgtttcg cgatcacggc gcgcttgccc gagacgggta tgcgctgctc
      541 tccctggccc gcgcggccaa ggtaagccgc ggcgggggtc tggggggtcg cggggccggg
      601 agccatggcc gccgcggacc cggtctgggc ctgagcgccc cctgcggtct gagcggggcc
      661 cgcggccagg ccctcgaggt ccctccgcac gaccctgccc ccggggccgc tgcccctcac
      721 cctggccagg tctatccccc gcgccgcggc tatggcccgg gcgagggggg aggatggcgg
      781 ggccttgccg ggagggaggc tcggatcctg ggcggccggg ccaggcccag gcgccgagtc
      841 caggctctgc ggccccggcg cagagcgggg ctgggggccc ggggcgctgc cggagggaac
      901 cgcggcgccc ttggcctgcg gcgcggcctt caccgatccc gggtcctcgc cctccttccc
      961 gatgacggct atcgtcgcgc cgacctcggc gctggagccg gccgccagga cgatcctgag
     1021 gaggaccccc gacttgggcg cctcgtagtc catggtggcc ttgtcggtct cgacctcgca
     1081 gagcacggcc cccgcggtga aggaggcgcc ctcggcgacc ctccagcgcg cgatcgtgcc
     1141 ctcgctcatc gtgggggaga gggcgatcat gtagaggttc tcggccatgg ccttcaggct
     1201 ccgaccgggt cgaggtagag gacgcggcgg gccgcggcca cgaccttggc ggcgtcgggc
     1261 tgggcggcca gctcgagggc gtgattgtag ggcatcggta cgtcctcccc ggacacgagc
     1321 tcgacggggg cgtcgagacg gtcgaagcag ttgcgcgaca ccagatgggc caggtgggag
     1381 cccacgctcg cctggggcca ggactcgtcg acgacgacgc agcggctggt cctcgagacc
     1441 gactcgtaga ccgtcccctc gtcgatgggc ctgaggctcc tgaggtcgat cacctcggcc
     1501 tctatcccct cggcggcgag gagctcggcc gcctcgagca cggccttcat gggcttggag
     1561 aaggagacga tgctgaggtc gcctccccgc ctgcgcaggg cggcctggcc gatggggacg
     1621 aggaactcct cctcggccac ctcgcccttc caggcgtaca tgagctcgct ctcgagcacc
     1681 acgacggggt cctcgtcgcg gatcgcggtc ttgaggaggc cgtaggcgtc cgagggtgtc
     1741 gagggggcga tgaccttgag cccgggcaca tgggcccagt agctggccag ggcctgggag
     1801 tgctggccgg cgaggtactc ggcggggccg ttggggccgc ggaagacgat ggggaccgag
     1861 agcctgcccc ccgacatgta gcgcatcttc gccgcctcgt tcaccacctg gtccagggcg
     1921 aggagggcga agttgtgcgt catccactcg acgacggggc ggagcccggc tatggccgcg
     1981 cccacgccca ccccggtgaa gccgagctcg gctatcgggg tgtcgcggac cctgtcgggc
     2041 ccgtacttgg ctaagaggcc cttggacacc ttgtaggccc cgtcgtactc gccgacctcc
     2101 tcgccgatca ggaagacccg gccgtccctg gccatctcct cgtcgagggc ccggttgagg
     2161 gcctcgcggt agctcatgac cgccatcatg gcctcccttc gccggacgag ccgggcacgg
     2221 cgcgccccgc cgggctggct ccggcttccc agagcacgtc ggtgaccgac gatccaagca
     2281 ggacgtcggt gaagagctcg gagggagggg gctcggggct ctcctcggcg aaggccacgg
     2341 cctcctcgac gatccgctcg gcctcggcca cgagggccga ccagtcggcc tcggccagga
     2401 gccctgcctc ctcgagcctg cccttgagca gggctatggg atcgcgtttg cggtactcgt
     2461 cgatgtcctc gcgggtgcgg tacttctgcg ggtcgctcat cgagtggccc ttgtagcggt
     2521 aggtcagggc ctcgacgagg ctcgggacgg cctccttccg cgcccgctcg actgccctgg
     2581 ccacgacgcg gcggacgtcg aggatgtcca tgccgtcgca ggcctccccg ggcatgtcgt
     2641 aggaggcggc ggtcgcggcg aggtcgtgga gggccgagac cttcttccag ctcgtgccca
     2701 tgccccactg gttgttctcg atgatgtaga cgatggggag gttccagatc ctcgccatgt
     2761 tgaggctctc gtggatcgcc ccctgacgga aggccccgtc gccgaggaag cagagggtgg
     2821 cgcccccgtc cctgcggtag gactgggcca gggccacgcc cgtcgcgatg ggcacctggg
     2881 cgccgacgat gccgttgccg ccgagcatgc ggcgctcggg gtcgaaaagg tgcatcgagc
     2941 cgcccttgcc cttggacacc ccgcccgact tgccgaagag ctcggccatg atggccttgg
     3001 gctccatgcc gcaggctatg gcgtggccgt ggtcgcggta ggcggtgagc acgtagtcct
     3061 tggccatatc gagcaccgag atggcgccga cggcgacggc ctcctggc
//
LOCUS       DASSQV010000521         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1365810, whole genome shotgun sequence.
ACCESSION   DASSQV010000521 DASSQV010000000
VERSION     DASSQV010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365810"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1073)
                     /gene="rbsK"
                     /locus_tag="VFL04_09150"
     CDS             complement(150..1073)
                     /gene="rbsK"
                     /locus_tag="VFL04_09150"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HET7839916.1"
                     /translation="MTKFCVVGSLNMDMVTRVERFPQPGETISGLSFNIFPGGKGANQ
                     AVALARLGARVEMVGALGDDVLGNRYAEILAELHIGRSAIARESGVSTGTASIEVSEA
                     GENHIVIVAGANALVAPAKVVAARPIIESCSSLLLQLEIPMDSVLEAARIAKAAGLAV
                     ILDPAPARELPEALYPLVSVITPNEHEARLLTGENTTTESGIARAGEKLCALGAACAI
                     IKAGPRGAFVVEGGRCRRIPGYKVKVVDTTAAGDSFNAGLALALGEGRELAEAVRFAN
                     AVGALSTTREGAQSAMPSLEEARAFMAAQAT"
     gene            complement(1101..1793)
                     /locus_tag="VFL04_09155"
     CDS             complement(1101..1793)
                     /locus_tag="VFL04_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7839917.1"
                     /translation="MEQDIVPIDLGFVNAFLLRAKDGFVLIDTGLPQQWEALDRALTA
                     AGCSRGKLALVVVTHGDLDHYGNCARLQKEYGAKVGVHREDAPVLRTGIGQERRISGL
                     IATVIMGVLHFFQRFGGPPQRWNCEPDLLLEDGQDLGPFGLEARVLALPGHTRGSIGV
                     LMATGDFFVGDLLGNRPRPAPSFYIENTANYRRSLERVQAMTGIGTVYPGHGRPFGPE
                     ALPGISLRPERV"
     gene            1879..2868
                     /locus_tag="VFL04_09160"
     CDS             1879..2868
                     /locus_tag="VFL04_09160"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HET7839918.1"
                     /translation="MDLKDFPRSELGFFPTPLVRLERLSKLLGGPRLLMKRDDLTGLA
                     TGGNKTRKLEYLVGEALALGCDCLVTGGAAQSNHCRQTAAAAAARGLGCHLVLGGSEP
                     ERYEGNLLLDRLLGAEIHWSGALRKGERIPEVAAGLEARGRRPYVVPYGGSNEVGALG
                     FVRAASELAEQLRAAGETVSRIVFASSSGGTHAGLVVGKALFGLGAQILGIGIDKGEA
                     GEMPFEDHVHELASSLASRLGLGRPIGRGEALVRNEFLGGGYGVVGEAEREAISLLAR
                     SEGILLDPVYTGKAFGALLALSRLGEFSKSETILFWHTGGTPALFSPAGQTLL"
BASE COUNT          459 a         1107 c         1086 g          484 t
ORIGIN      
        1 ctcccgcgag gtcttcgccg acgagttcaa tgacccccat ctggtcctgc cctcgatcgg
       61 cgacgaggtc gtgctcagct tcgcccgaga ggcctgctcg gtcgccacga gcgaggccgc
      121 gcccgtcgtg gcggacgccg aggactgaat caggtcgcct gggcggccat gaaggcccgg
      181 gcctcctcga ggctcggcat ggcgctctgg gcgccctccc gcgttgtgga cagggcgccc
      241 acggcgttgg cgaaacgcac cgcctcggcc agctccctgc cctctccgag ggccagtgcc
      301 aggccggcgt tgaaggagtc gccggcggcc gtcgtgtcga cgaccttgac cttgtagccg
      361 gggatcctcc ggcagcgccc accctcgacg acgaaggctc cgcgcgggcc agccttgatg
      421 atggcgcagg ccgcacccaa ggcgcagagc ttttcccccg cgcgcgcgat gcccgactcc
      481 gtcgtggtgt tctccccggt aagtagcctc gcctcgtgct cgttgggggt gatgaccgag
      541 accagcggat agagggcctc ggggagctcc cgggctgggg ccggatccag gatcacggcg
      601 aggccggccg ccttcgcgat cctcgcggcc tcgagcaccg agtccatggg gatctcgagc
      661 tgcagcagga ggctggagca ggactcgatg atgggtcggg cggcgacgac cttggcagga
      721 gccacgaggg cgttcgcgcc agcgacgatg acgatgtggt tctcaccggc ctcggacacc
      781 tcgatgctcg ccgtcccggt cgagaccccg ctctcccgcg cgatcgccga ccttccgata
      841 tggagctcgg cgaggatctc ggcgtagcgg ttgccgagga cgtcgtcgcc cagggcgccc
      901 accatctcaa cccgggcgcc gagcctcgcg agggccacgg cctggttggc ccccttgccg
      961 ccggggaaga tgttgaacga aaggccgctg atcgtctcgc ccggctgggg gaagcgctcc
     1021 acgcgggtca ccatgtccat gttgaggctt ccgacgacgc agaatttcgt catgctgttc
     1081 ggctccttag ggcccagagc ctacaccctt tccggcctca gggaaatgcc cggcagggcc
     1141 tcagggccga agggccggcc gtggccgggg tagaccgtgc ctatgccggt catggcctgg
     1201 accctctcga gcgagcgccg gtaattggcg gtgttctcga tgtagaagga gggggcgggt
     1261 ctcggccggt tgcccaggag gtcgcccacg aagaaatcgc ccgtggccat gagcacgccg
     1321 atggagcccc gcgtgtggcc cggcagggcc aggaccctcg cttcgaggcc gaagggtccg
     1381 aggtcctggc cgtcctcgag gaggaggtcg ggctcgcagt tccacctctg gggcgggcct
     1441 ccgaagcgct ggaagaagtg caggacgccc atgatgaccg tggcgatgag gcccgagatc
     1501 ctccgctcct gtccgatccc cgtcctgagg acgggggcgt cctcgcggtg cacgcccacc
     1561 ttcgctccgt actccttctg aagcctcgcg cagttgccgt agtggtcgag gtcaccgtgg
     1621 gtgacgacga cgagggcgag cttgccacgg ctgcagccgg cggcggtgag ggccctgtcc
     1681 agggcttccc actgctgggg gaggcccgtg tcgatgagga cgaagccatc cttcgccctc
     1741 aggaggaagg cgttcacgaa tcccaggtcg atgggcacga tgtcctgttc catgttccct
     1801 cccacgatac cctctacgat aattccctgc gtggcccctg tccaatgcgg tatactggcc
     1861 gtctcggagg cgacggccat ggacctgaag gacttccccc gcagcgagct cggctttttc
     1921 ccgacaccgc tcgtgcggct cgagcggctc tcgaagctgc tcggcggacc gcggctcctc
     1981 atgaagcgcg acgacctcac cggcctcgcg accgggggca acaagacgag gaagctcgag
     2041 tacctcgtgg gcgaggccct ggccctcggc tgcgactgtc tcgtcaccgg cggggcggcc
     2101 cagtccaacc actgccgcca gaccgctgcc gcggccgcgg cgcgcggcct gggctgccat
     2161 ctcgtgctcg ggggaagcga gccggagcga tacgagggca acctcctcct cgaccgcctc
     2221 ctcggcgccg agatccactg gtcgggcgcg ctgcggaagg gcgagcgcat ccccgaggtc
     2281 gccgcggggc tcgaggccag gggccgccgg ccctacgtcg tgccctacgg cggctccaac
     2341 gaggtcggcg ccctcggctt cgtgcgcgcc gcctctgagc ttgccgagca gctcagggcc
     2401 gctggcgaga ctgtgtcgcg catcgtgttc gcctcgagct cgggagggac ccacgccggc
     2461 ctcgtcgtcg gcaaggccct cttcggactg ggcgcccaga tcctcggcat cggcatcgac
     2521 aagggcgagg cgggcgagat gcccttcgag gaccacgtgc acgagctcgc ctccagtctg
     2581 gcctcaaggc tcggcctcgg gcgccccatc ggccgcggcg aggccctcgt ccgcaacgag
     2641 ttcctcggcg gaggctacgg ggtggtgggc gaggccgagc gcgaggcgat cagcctcctc
     2701 gcccgcagcg agggcatcct cctcgacccc gtctacacgg gcaaggcctt cggcgccctc
     2761 ctcgccctca gccggctcgg ggagttctcc aagagcgaga ccatcctctt ctggcacacc
     2821 gggggcacgc cggcgctttt ctcgcccgcc ggccagaccc tcctctgaac acggagggga
     2881 tggcctagtc ggctccaggg cttgaccctg ccgcgcgggc ggcgctatct tggagccaga
     2941 caccccaccc cccgtgggga ggggtgtctg gaggcggcaa gcgatggatc attccagcca
     3001 cgagagcgcg aaggcactcg aaaccctcag gacggccaag ggccaggtcg aggccatcat
     3061 ccgcatggtc gaggacgacc gctactgcat cgacgtctcg aaacaggtcc acgccgcgat
     3121 cgccctgctc aagaag
//
LOCUS       DASSQV010000522         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_246967, whole genome shotgun sequence.
ACCESSION   DASSQV010000522 DASSQV010000000
VERSION     DASSQV010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246967"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /gene="serC"
                     /locus_tag="VFL04_09165"
     CDS             complement(<1..760)
                     /gene="serC"
                     /locus_tag="VFL04_09165"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HET7839919.1"
                     /translation="MKRVINYSAGPAAIPLEVLEQARDEMLDWNGTGCSVMEVSHRGK
                     EYDALHHETMDLFREVAGMGKEWHVLFTTGGASTQFFMLPMNYLGEGKQADYIVTGNW
                     SKAAVKEAKRYGKISHPANMELEGEVFKAIPKPGDLKFNSAAEYVHYTTNNTVCGTEW
                     FYTPDTKGKPLVADMSSDIFSRPMEFDKHSMIYAGAQKNLGPSGVTVVAIKDEFLAKA
                     KDDLPTMLSYKTHATNNSLYNTPPCFSIYILDLTL"
     gene            complement(770..1645)
                     /locus_tag="VFL04_09170"
     CDS             complement(770..1645)
                     /locus_tag="VFL04_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HET7839920.1"
                     /translation="MVILMPDAFASDLPGRLAPFGEVTSDLGKLGEAEVIIVRSKTKV
                     DKAMIDKAPKLKYVIRGGVGVDTIDVEYAKQKGIAVDNTPEASSLAVAELAFALMIAL
                     PNHIAEADASMKQGKWLKKELERTELGGKTLGLVGIGRIARMLAVRAAAFDMKVIAFD
                     KYVTDCGDVAKMVSLSEVLASSDYISLHTPLTDETRGMINAASIAKMKKGAYIINTCR
                     GPVVNTADMAAALAEGRIGGYATDVFDKEPPEGSPLLTAPRVLLTPHLGASTEENLVR
                     LGDCIVERMAKYAKK"
     gene            complement(1801..>2230)
                     /locus_tag="VFL04_09175"
     CDS             complement(1801..>2230)
                     /locus_tag="VFL04_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HET7839921.1"
                     /translation="EALKCCYDIKLERCASPEKAFAIIDASDTAAREQKVALTSAKGS
                     GIISFPDPRANMAAGTLQDFLDGFLKDNPQVVIDYIHGTESMLSLAAKPGNLGLYLPP
                     IDKGGFFGTVIRDGVMPRKTFSMGEAPEKRFYIEARRITK"
BASE COUNT          324 a          643 c          808 g          455 t
ORIGIN      
        1 cgagggtgag gtcgaggatg tagatggaga agcagggcgg cgtgttgtag agcgagttgt
       61 tcgtcgcgtg ggtcttgtag gaaagcatcg tgggcaggtc gtccttggcc ttggcgagga
      121 actcgtcctt gatggccacg acggtgacgc ccgaggggcc gaggttcttc tgggcgcccg
      181 cgtagatcat ggagtgcttg tcgaactcca tggggcggct gaagatgtcg ctcgacatgt
      241 cggcgacgag gggcttgccc ttcgtgtcgg gggtgtagaa ccactcggtg ccgcacaccg
      301 tgttgttcgt ggtgtagtgc acgtactcgg ccgcactgtt gaacttgagg tcgccgggct
      361 tgggtatggc cttgaagacc tcgccctcga gctccatgtt cgcggggtgg ctgatcttgc
      421 cgtagcgctt ggcttccttg accgcggcct tggaccagtt gccggtgacg atgtagtcgg
      481 cctgcttgcc ctcgccgagg tagttcatgg ggagcatgaa gaactgggtg gaggcgccgc
      541 cggtggtgaa gaggacgtgc cactccttgc ccatgcccgc gacctcgcgg aagaggtcca
      601 tggtctcgtg gtggagggca tcgtactcct tgccgcggtg cgagacctcc atgacggagc
      661 agccggtgcc gttccagtcc agcatctcgt cgcgggcctg ctcgaggacc tcgaggggga
      721 tcgccgccgg tccggcgcta tagttgatca ctcgcttcat gtctctctcc tacttcttcg
      781 cgtacttggc catgcgctcg acgatgcagt cgccgaggcg gacgaggttc tcttctgtcg
      841 aggccccgag gtggggggtc aggaggaccc ggggggcggt gaggaggggc gagccctcgg
      901 gcggctcctt gtcgaagacg tcggtggcgt agccgccgat gcggccctcg gcgagggcgg
      961 cggccatgtc ggcggtgttc accaccgggc cgcggcaggt gttgatgatg taggcgccct
     1021 tcttcatctt cgcgatcgag gcggcgttga tcatgcccct ggtctcgtcg gtgagggggg
     1081 tgtgcagcga gatgtagtcc gagctcgcga ggacctcgga gaggctgacc atcttggcca
     1141 cgtcgccgca gtcggtgacg tacttgtcga aggcgatcac cttcatgtcg aaggcggcgg
     1201 ccctgacggc gagcatgcgg gcgatgcggc cgatgccgac gaggcccagg gtcttgccgc
     1261 cgagctcggt gcgctcgagc tccttcttga gccacttgcc ctgcttcatc gaggcgtcgg
     1321 cctcggcgat gtggttgggg agggcgatca tgagggcgaa ggcgagctcg gcgaccgcga
     1381 ggctcgaggc ctcgggggtg ttgtcgacgg cgatgccctt ctgcttggcg tactccacgt
     1441 cgatcgtgtc cacgccgacg ccgccgcgga tgacgtactt gagcttgggc gccttgtcga
     1501 tcatcgcctt gtcgaccttg gtcttggagc ggacgatgat gacctcggcc tcgccgagct
     1561 tgccgagatc ggacgtgact tccccgaagg gcgcgagcct tcccggcagg tccgacgcga
     1621 aggcgtcggg cataagaatg accatggatt cctccctatg atggcgtggt agttcccaaa
     1681 gcggatgtat tcgagtgtgc cggttattct aacacgggat ccgttactgt caacatattg
     1741 ccgagcaaac cggccgtttc gccgagcgaa acggccggcg cccattcctg ccgcattccc
     1801 ctacttcgtt atccgccgcg cttcgatgta gaaccgcttc tcgggcgcct cgcccatcga
     1861 gaaggtcttg cgcggcatca ccccgtcgcg gatcaccgtg ccgaagaagc cgcccttgtc
     1921 gatcgggggc aggtagagcc cgaggttgcc gggcttggcg gcgagggaga gcatgctctc
     1981 ggtgccgtgg atgtagtcga tgacgacctg ggggttgtcc ttgaggaagc cgtcgaggaa
     2041 gtcctggagg gtcccggcgg ccatgttggc cctggggtcg gggaaggaga tgatccccga
     2101 gcccttggcg gaggtgaggg ccaccttctg ctcgcgggcc gccgtgtccg aggcgtcgat
     2161 gatggcgaag gccttctcgg gcgaggcgca gcgctcgagc ttgatgtcgt agcagcactt
     2221 gagggcctcg
//
LOCUS       DASSQV010000523         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1614885, whole genome shotgun sequence.
ACCESSION   DASSQV010000523 DASSQV010000000
VERSION     DASSQV010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614885"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..639)
                     /locus_tag="VFL04_09180"
     CDS             complement(283..639)
                     /locus_tag="VFL04_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839922.1"
                     /translation="MDGGLGWNRGAGLVAMRLGCWPGLWLGCSQRLLGSGFLASELPD
                     LAKPLAELLGRHSRVKARLLDVFRKLIALLDRQLGDQRGIPLLPALFRHLVRSLARSP
                     FFAEQLLSLVDPRFGL"
     gene            787..1257
                     /locus_tag="VFL04_09185"
     CDS             787..1257
                     /locus_tag="VFL04_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839923.1"
                     /translation="MSGLEDKLDGLIRRIKEAAEHASTASEEARRPYGLLAQGERQRA
                     LSAARPEDPRRSKEGRRSGHERRGAPERRSPADRRSSDRRVAEDRRLAPERRQGDRRS
                     KPPIVPSLGRAAAAIRDLRSGPEGLPTEIAGMPVSPRLARLIEIVRREKTHGKG"
     gene            1244..2110
                     /locus_tag="VFL04_09190"
     CDS             1244..2110
                     /locus_tag="VFL04_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET7839924.1"
                     /translation="MAKDNAARLREYVGGMPSLPTTVSKVLEVCNNPKTSPIDLDKVI
                     QLDPVLMGRVLKLINSAYYGLGTQVTSLVRAIIMLGVNTVKNLALSTAILDRVANKGD
                     FRALNMEGFWRHSLCVGVTAKLIAKRRGIDAKTLEEYFASGLLHDIGKIPLNNALAED
                     YVRTMSVADREKVSLFDAEGRTLGIDHCEAGRIVAELWKLSGAIADTAAWHHDLKGYT
                     GEHGDVLRTVAVANYFANREGIGFSGNLYPEKIDPEIYELLGIEKSLLIEIEEPVNAE
                     IEKAKVFLRIGG"
     gene            2114..>2632
                     /locus_tag="VFL04_09195"
     CDS             2114..>2632
                     /locus_tag="VFL04_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7839925.1"
                     /translation="MFTLKIWGDRGSMPTPGPDTVIFGGNTSCLEVRCDDRLIIIDAG
                     SGVRQLGDKLMREDIRKGPIAADILITHTHWDHIMGFPMFTPIYVPGTRLKIRGPISY
                     EDDTLEKVMGSQLSYRYWPVRQEELAAKIEYEQIKETTLDLGDGIYVTTKYLNHPILC
                     LGYRIEYEGKSFV"
BASE COUNT          503 a          883 c          899 g          347 t
ORIGIN      
        1 gcggtgatgc cccccccggc cccacagcag ggaccccaat tccagccgca gcctgccgcg
       61 ccccaagcct catggggaca gggtccaagc cgtccggtgc cgccggactt cggggccgag
      121 cccgagccca tccctgcccg acgcgagccc gagccgccga ggagggccca ggagcccttc
      181 cggccggagc cggggcctgg ctggcccgat gaggagagcc agccggagga agccgggcgg
      241 gtgccagagg aagcgctcgg actgggcgag gaggccgagg cctcagagcc cgaaccgggg
      301 atccacgagg ctcaggagct gctcggcgaa gaaggggctt cgggcgaggc tccggacgag
      361 gtggcggaag agggcgggga ggagggggat gccacgctgg tccccgagct ggcggtcgag
      421 gagagcgatg agcttccgga atacatcgag gagccgagct ttgactcggc tgtggcggcc
      481 gaggagttcg gcgaggggct tggcgaggtc ggggagctcg gaggccagga atccggaacc
      541 gaggagcctc tgcgagcacc ccagccagag ccccggccag cagcccaggc gcatcgcgac
      601 gagcccggcg ccccgattcc agcccagccc accgtccaag ccccgggctc cgacagccca
      661 agggggcatg gggaggccga agcggcgccc gaggtccccg tcatcggcga ggcggccgcg
      721 aatctgtttg agtatctgaa gggcctgtcg aaggagctcc cggccgacaa actcgaggag
      781 ttcgccatgt ccgggctcga ggacaagctc gacggcctca tcaggcggat caaggaggca
      841 gccgaacacg cctccacagc ctcggaggaa gccaggcggc cctacggcct ccttgcccag
      901 ggggagcggc agcgcgccct ctccgcggcc aggcctgagg atccgcggcg ctcgaaggag
      961 ggccggaggt cgggccacga aaggcggggc gctcccgaga ggcgctcgcc cgcggaccgg
     1021 cgatcctccg atcggcgcgt ggccgaggac cgccgcctgg ccccggagcg ccgccaggga
     1081 gacagacgca gcaagcctcc gatcgtgccg agccttggac gcgccgcggc cgcgatccgc
     1141 gacctccggt ccgggcccga ggggctgccg accgagatag cggggatgcc ggtctccccg
     1201 cggctcgcgc ggctgatcga gatcgtgcgt cgggagaaga cacatggcaa aggataacgc
     1261 ggcacgcctg cgggagtatg tcggcggcat gcccagcctc cccaccacgg tttcgaaggt
     1321 cctcgaggtc tgcaacaacc ccaagaccag cccgatcgac ctcgacaagg tcatccagct
     1381 cgaccccgtc ctcatgggaa gggtgctcaa gctgatcaac tcggcctact acggcctcgg
     1441 gacccaggtc acgagcctcg tacgggccat catcatgctc ggggtgaaca cggtgaaaaa
     1501 cctggccctg agcacggcga tcctggaccg ggtggcgaac aagggcgatt tccgggccct
     1561 gaacatggag ggcttctggc gccattccct ctgcgtcggc gtcaccgcga agctcatagc
     1621 caagcgccgc ggcatcgacg ccaagaccct cgaggagtac ttcgcctcgg gcctcctcca
     1681 tgacatcggc aagatccccc tcaacaacgc cctggctgag gactatgtca ggaccatgtc
     1741 ggtcgccgac cgcgagaagg tgtccctgtt cgacgccgag ggaaggaccc tcggcatcga
     1801 ccactgcgag gcaggccgca tcgtcgccga gctgtggaag ctgtccggcg cgatagccga
     1861 cacggcggcc tggcaccacg acctcaaggg ctacacgggc gagcacggcg atgtcctgcg
     1921 cacggtcgcc gtcgccaact acttcgccaa ccgggaaggg atcggattct cgggaaacct
     1981 ctatcccgag aagatcgacc ctgagatcta tgagctcctg gggatcgaga agtcgctcct
     2041 catcgagatc gaggagccgg tcaacgcaga gatcgaaaag gccaaggtct tcctgaggat
     2101 cgggggctag ggaatgttca cgctcaagat ctggggggac cgcggctcca tgcccacccc
     2161 gggacccgac accgtcatct tcggcggcaa tacctcatgc ctcgaggtcc gctgcgacga
     2221 caggctcatc atcatcgacg cgggctcggg ggtcaggcag ctgggcgaca agctcatgcg
     2281 ggaggacatc aggaagggcc ccatcgcggc cgacatcctg atcacccata cccattggga
     2341 ccacatcatg ggcttcccca tgttcacgcc gatctatgtg ccgggcacga ggctcaagat
     2401 ccggggaccc atcagctatg aggacgacac cctcgagaag gtcatgggat cccagctctc
     2461 ctaccggtac tggccggtgc gacaggagga gctggccgcg aagatcgagt acgagcagat
     2521 caaggagacg accctcgatc tcggcgacgg gatctacgtc acgaccaagt acctgaacca
     2581 tcctatcctt tgcctgggat atcgcatcga gtacgagggc aagtccttcg tc
//
LOCUS       DASSQV010000524         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_247320, whole genome shotgun sequence.
ACCESSION   DASSQV010000524 DASSQV010000000
VERSION     DASSQV010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247320"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1188
                     /locus_tag="VFL04_09200"
     CDS             244..1188
                     /locus_tag="VFL04_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7839926.1"
                     /translation="MFTYSLKRIAQSAMTLLIVITFVFLLMRLLPIEGYFGDMYDKLS
                     PSQREAILNKMGLLDPWPVQLGKFYASLARGDLGVSTIYRKDVSVAEVIGPRVPYSVY
                     FGLVATAISLVAGLAMGVHMARHKGRFWDKTWTVYVVFINAVPLAVYLLFIQLYGSDL
                     MKLPILFDKHRPVSWILPAVSMSLAGIAGYAMWMRRYMVDELNRDYIKLARAKGMTTS
                     AIMVKHVMRNAYVPMAQYLPAAILATISGSIYIESLYSIPGMGGLLVDVIQRQDNPMV
                     QALVLIFSSVGILGLFLGDLLMALFDPRIKFVKSEGAR"
     gene            1188..2189
                     /locus_tag="VFL04_09205"
     CDS             1188..2189
                     /locus_tag="VFL04_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7839927.1"
                     /translation="MAAKPAPAAAAAACPPGPGAPALSDAELFGFAEFDPNAGEKVAW
                     SNYSYWRSTLRVFLRNRISLILLVSLAALLFFTFIQPFLPYQKNPTTIYLDPSTGRQF
                     RNVAPGQEFWFGTNSIGQDLWSRIWSGTRTSLLIGILVGLVEMVIGIVYGALWGYVRR
                     LDRALTEIYNVLNNIPTTIILMLLAYIMKPSFRTMVFAMCASGWIGMARFIRNQIVII
                     RDREYNLASRCLGTPSGRIISKNLLPYLVSVIMLQTALSIPGTIGWEVFLTYIGIGLP
                     VSIPSLGNLVNEGRRIMMSPELRYQLIFPTIVTSFITVSLYVLGNSFSDASDPRNHV"
     gene            2303..>2944
                     /locus_tag="VFL04_09210"
     CDS             2303..>2944
                     /locus_tag="VFL04_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7839928.1"
                     /translation="MSDLVVKFSLRGRVLTAIRGASLELHEGETLAIVGESGSGKSVF
                     TKTFIGMLDQNGWVDSGSILFEGRDLAGYSTEEEWLGIRGKKISMVFQDPMTALNPLR
                     SVGRQIAEVIELHQGATAAEAELQAIELLERVGIPEPGTRHRQYPHEFSGGMRQRVVI
                     AIAMACKPRILICDEPTTALDVTIQAQILGLIKALQRDFGFTTIYITHDLGVVA"
BASE COUNT          510 a         1073 c          842 g          519 t
ORIGIN      
        1 tcggcaccgc cgaataccag aagctcgagg cccagtggct caaggaaaaa gcggccgccc
       61 tcaccaaggt caagtagagg aaagaagaga accgcagaga cgcagaggcg cagagctggg
      121 gagggagggt ggaggagaag ctccactcct acctccgggc tctgtgcact ctgtggttca
      181 tcgctcagtt cccatcaggc agcatcgagc gcgggaggat ggcccgtagg ggccacacaa
      241 ggcatgttca catactcgct taagcgcatc gcccaatcgg ccatgaccct cctcatcgtc
      301 atcaccttcg tcttcctcct catgcggctc ctgcccatcg agggctattt cggggacatg
      361 tacgacaagc tctccccgag ccagagggag gccatcctca acaagatggg cctgctcgac
      421 ccctggcccg tccagctcgg gaagttctac gcctccctcg cccgcggcga cctcggcgtc
      481 tccaccatct accgcaagga cgtgagcgtg gccgaggtga tcggcccgcg cgtgccctac
      541 tccgtgtact tcggcctcgt cgccaccgcg atctccctcg tggccggcct cgccatgggc
      601 gtccacatgg ccagacacaa gggccggttc tgggacaaga cctggaccgt ctacgtcgtg
      661 ttcatcaacg ccgtgccgct cgcggtctat ctccttttca ttcagcttta cgggagcgac
      721 ctcatgaagc ttcccatact cttcgacaag cacaggccgg tgagctggat cctgccggcg
      781 gtctccatgt cgctcgctgg catcgcgggc tacgcgatgt ggatgcgccg ctacatggtg
      841 gacgagctca accgcgacta cataaagctc gccagggcca agggcatgac cacctcggcg
      901 atcatggtca agcacgtcat gcggaacgcc tatgtgccca tggcccagta cctgcccgcc
      961 gccatcctcg cgacgatctc cggctcgatc tacatcgagt cgctctactc gatccccggc
     1021 atgggcgggc tcctggtgga cgtgatccag aggcaggaca acccgatggt ccaggcactg
     1081 gtcctcatct tctcctcggt gggcatcctc ggcctattcc tcggcgacct cctcatggct
     1141 ctcttcgatc cgaggatcaa gttcgtgaag agcgaggggg caaggtaatg gccgccaagc
     1201 ccgcccccgc ggccgccgcg gccgcctgcc ccccagggcc cggagcgccg gccctcagcg
     1261 acgccgagct cttcggcttc gccgagttcg accccaacgc cggcgagaag gtggcctggt
     1321 cgaactactc ctactggcgc tccaccctcc gcgtcttcct ccgcaaccgg atctccctca
     1381 tcctcctcgt gtccctcgcc gccctccttt tcttcacctt catccagccc ttcctcccct
     1441 accagaagaa cccgacgacg atctacctcg atccctctac tggcaggcag ttcaggaacg
     1501 tcgctccggg gcaggagttc tggttcggga ccaactccat aggccaggac ctctggtcga
     1561 ggatctggag cgggacaagg acctccctcc tcatcggcat cctcgtgggc ctcgtcgaga
     1621 tggtcatcgg catcgtctac ggcgccctct ggggctacgt gcgccgcctg gaccgggccc
     1681 tgaccgagat ctacaacgtc ctcaacaaca tccccacgac catcatcctc atgctgctgg
     1741 cctacatcat gaagccgagc ttcaggacca tggtcttcgc gatgtgcgcc tccggctgga
     1801 tcggcatggc ccgcttcatc cgcaaccaga tcgtcatcat ccgcgaccgg gagtacaacc
     1861 tcgcctcgcg ctgtctcggc accccctcgg gcaggatcat ctccaagaac ctcctgccct
     1921 atctcgtgtc ggtcatcatg ctccagaccg ccctctccat cccgggcacg ataggctggg
     1981 aggtcttcct cacctacatc ggcatcggcc tgccggtgag catcccctcg ctcggaaacc
     2041 tcgtcaacga aggcaggcgc atcatgatgt cgcccgagct ccgctaccag ctgatcttcc
     2101 ccacgatcgt gacctccttc atcacggtct cgctctacgt cctcggcaac tccttctccg
     2161 acgcctcgga cccgaggaac cacgtatgag cgccgccgac cgctcaaagt tgggcgcggt
     2221 cgcccgcgaa gcgcccgcgc cccggcctga cgcgggcgcg ggtggagcgc agcaaggcgg
     2281 aagcgcgtca ccgattctct cggtgagcga cctcgtcgtg aagttcagcc tccgcggccg
     2341 cgtcctcacg gcgatccgcg gggcgagcct cgagctccac gagggcgaga ccctcgccat
     2401 cgtcggcgag tcgggctcgg gcaagtcggt gttcaccaag accttcatcg ggatgctcga
     2461 ccagaacggc tgggtcgact cgggctccat cctcttcgag ggccgggacc tcgccggcta
     2521 ctccaccgag gaggagtggc tcggcatccg cggcaagaag atctccatgg tcttccagga
     2581 cccgatgaca gccctcaacc ccctgcggag cgtgggcagg cagatcgccg aggtgatcga
     2641 gctccatcag ggggccacgg ccgccgaggc cgagctccag gccatcgaac tgttggagcg
     2701 cgtgggcatc cccgagccgg ggacgaggca ccgccagtac ccccacgagt tctcgggggg
     2761 catgaggcag cgggtggtca tcgccatcgc catggcctgc aagccgcgga tcctcatctg
     2821 cgacgagccc accacggccc tcgacgtgac catccaggcc cagatcctcg gcctcatcaa
     2881 ggccctgcag cgggacttcg gcttcaccac gatctacatc acccacgacc tcggcgtggt
     2941 cgcc
//
LOCUS       DASSQV010000525         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1240094, whole genome shotgun sequence.
ACCESSION   DASSQV010000525 DASSQV010000000
VERSION     DASSQV010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240094"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VFL04_09215"
     CDS             complement(<1..952)
                     /locus_tag="VFL04_09215"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HET7839929.1"
                     /translation="MKHKISPARLIAAALLLGAQAALFPLNLPKSVGAALPKPSAEVT
                     SFQLEAISLRDVSFRFVLAVKNPYPVALKFDGMTLDFSVEGAKVFTVASQGGFSVGAK
                     SERSNAFTVALSYESVIKLVKDYVQKDWLNTVIDGSLVIPIPKIDSMPGLPPNVSFSY
                     RFQKRIPAIKPQVAILDFSVKPPTEAQIAEAIAKAGRKVDKGKALGVFKNVLAGKKPA
                     EPVIDPAELDVPLVVSFTIAIRNEAKGPISFDRLGYELAVNQERLVAGESAKVVRDGD
                     RTLITVDNSFSSRQLSKGIKAVFADRKGAFSVKGSASIKLP"
     gene            complement(1023..>2457)
                     /locus_tag="VFL04_09220"
     CDS             complement(1023..>2457)
                     /locus_tag="VFL04_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HET7839930.1"
                     /translation="VQASSATRSYGYGLWLRPASDGRFTPYFQGSDPGVSFLSSHDPS
                     TGRTITLVSNLGDDVWGLEAEIGRVLDAGPAAYSGPRAARSEAGGSGRPGFRAPRPFT
                     IHLEDEVLADLRARIRSTRWPEPSPGPAWAQGTDLDYLKAILAYWAEGYDWRAREREL
                     NAFSHFRAEVDGTAVHFVHERAKGGGGIPLILGHGWPSSFVELLPLVPLLTDPAAHGI
                     EGPSFDLVIPSLPGYGFSPRPRRAGLDYRQVAGIWASLMRGLGYERYGAGGGDFGAGV
                     ASHLAMKEPGSVIGLHLSNLEIPPYCGAGSRPLSTAEAAYLARNLEWRREEGGYREIQ
                     GTKPQTLAYALNDSPAGLASWILEKWRSWTDSGGDLDARFGRDFLLTTLTLYWATGTI
                     GSSIRDYYDNRHFGGATGPRDFVGVPTGIALFSRSFVDEGTPPREWAERLYDIRRWTP
                     MPRGGHFAPIEEPELLARDIAAFFGAL"
BASE COUNT          378 a          779 c          909 g          391 t
ORIGIN      
        1 cggggagctt gatcgaagcc gagcccttga ccgagaaggc gccctttctg tccgcgaaga
       61 ccgccttgat gcccttcgac agctgtctgg agctgaagct gttgtccacc gtgatgaggg
      121 tcctgtcccc atcgcgcacg accttcgcgc tctccccggc gacgagcctc tcctggttca
      181 cggccagctc gtagccgagc ctgtcgaagg agatggggcc cttggcctcg ttcctgatgg
      241 cgatggtgaa ggagaccacg aggggcacgt cgagctcggc ggggtcgatg acgggctcgg
      301 caggcttctt gccggcgagg acgttcttga agacgccgag ggccttgccc ttgtccacct
      361 tcctgcccgc cttggcgatc gcctcggcga tctgggcctc ggtcgggggc ttcaccgaga
      421 agtcgaggat cgcgacctgg ggcttgatcg ccgggatccg cttctggaac ctgtaggaga
      481 agctcacgtt gggcggaaga ccgggcatcg agtcgatctt ggggatcggg atgacgaggc
      541 tcccgtcgat caccgtgttg agccagtcct tctggacgta gtccttcacg agcttgatga
      601 ccgactcgta gctgagcgct acggtgaagg cgttggacct ctcgcttttg gcccccaccg
      661 agaagccccc ctggctcgcc acggtgaaga ccttggctcc ctcgacgctg aagtcgaggg
      721 tcatgccgtc gaacttgagg gcgaccgggt aggggttctt gacggcgagc acgaagcgga
      781 agctgacgtc cctgaggctg atggcctcga gctggaatga ggtgacctcg gcgctgggct
      841 tgggcagggc cgccccgacg ctcttgggca gattgagcgg gaacagggcc gcctgggccc
      901 cgaggagcag ggccgcggcg atgagccggg ccgggctgat cttgtgcttc atggtgaggg
      961 ccttcctccg cagcaaagta tagagcgcgg ccgagggaaa tgcaccacct aggcccgagg
     1021 gcctagaggg cgccgaaaaa ggctgcgatg tcgcgcgcga ggagctcggg ctcctcgatg
     1081 ggggcgaagt gcccgcccct cggcatcggg gtccagcgcc gtatgtcgta gagccgctcg
     1141 gcccattccc gggggggagt cccctcgtcg acgaaggagc gggagaagag ggcgatcccc
     1201 gtcgggaccc cgacgaagtc ccgcggcccg gtggccccgc cgaagtgcct gttgtcgtag
     1261 tagtcgcgga tcgaggagcc gatcgtcccg gtggcccagt agagggtcag ggtggtcagg
     1321 aggaagtcgc ggccgaagcg cgcgtcgagg tctccgcccg agtcggtcca ggaccgccat
     1381 ttctcgagga tccatgaggc gagcccggcc ggcgaatcgt tcagggcata ggccagggtc
     1441 tggggcttcg tgccctggat ctcccggtat ccgccctcct cccgcctcca ctccaggttg
     1501 cgggcgaggt aagcggcctc ggccgtcgac aggggcctcg agccggcgcc gcaatagggg
     1561 gggatctcca ggttgctcag gtggaggccg atcaccgagc cgggctcctt catggccagg
     1621 tggctggcca ccccggcccc gaagtccccg ccccctgcgc cgtagcgctc gtagcccagg
     1681 ccgcgcatga ggctggccca gatccccgcg acctggcggt agtccaggcc cgcgcggcgg
     1741 ggccggggcg agaagccata gccgggcagg gaggggatga cgaggtcgaa ggacgggccc
     1801 tcgatcccgt gcgcggcggg atcggtgagg aggggcacga ggggcagaag ctcgacgaag
     1861 gagctcggcc agccgtggcc gaggatgagg ggtatgcccc ctcccccctt ggcacgctcg
     1921 tgcacgaagt ggaccgcagt cccatcgacc tcggcccgga aatggctgaa ggcattgagc
     1981 tcccgctcgc gcgcgcgcca gtcgtagccc tcggcccagt atgcgaggat ggccttgagg
     2041 tagtccaggt cagtgccctg ggcccaggcc gggccgggcg atggctccgg ccaacgcgtt
     2101 gaacggatgc gggctctgag gtcggcgagg acttcgtctt cgagatggat cgtgaagggc
     2161 ctcggggccc gaaagcccgg ccgccctgat cccccggcct cggagcgggc tgcccggggg
     2221 cctgagtagg ccgccgggcc agcgtcgagg actcggccga tctcggcctc gaggccccag
     2281 acgtcgtcgc cgaggttgct cacgagggtg atggtcctcc ccgtggaggg gtcatggctc
     2341 gagaggaagc tcacgccggg gtcgctgccc tggaaatagg gcgtgaagcg gccatcggaa
     2401 gcgggcctga gccagagccc gtagccgtag ctcctcgtgg ctgaggaggc ctggacg
//
LOCUS       DASSQV010000526         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1489672, whole genome shotgun sequence.
ACCESSION   DASSQV010000526 DASSQV010000000
VERSION     DASSQV010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489672"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFL04_09225"
     CDS             complement(<1..710)
                     /locus_tag="VFL04_09225"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET7839931.1"
                     /translation="MLLALDARNRSVTVGLRDGGDWLTIRRLGSDRERTADEYALLLQ
                     AVREAAPASGKAIDSAWISSVVPALTPRLVEAVASAFGLQASVIGPGVRTGVKIRTDL
                     PSEVGSDLVCVAAAARELVKGACVVVDFGAAIAFSAINAAGEFLGAAIAPGIETAQAA
                     LRSTAAQIPDVRLEPPRFAIGRNTAASIQSGILLGYSGLVSRLVGLMREELGAAHGGS
                     GGSSSVDVIGTGDALGRG"
     gene            complement(717..1289)
                     /locus_tag="VFL04_09230"
     CDS             complement(717..1289)
                     /locus_tag="VFL04_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7839932.1"
                     /translation="MATSSPVRKVVVAGALGAVSIALVLLNLGFITWFAGASITVLHV
                     PVIIAAVLEGPVVGMAVGAIFGLTALLKAATAPQGPIDLFFVNPLISVLPRILLGLAA
                     WAYFRLFWGRMTGLAATVAGVAGSITHSVLVLGALVLFGAIPIAVAASVFVANGIIEA
                     AVAGVLTLAVVAAWKGIESRTGKARLADEG"
     gene            complement(1344..2210)
                     /locus_tag="VFL04_09235"
     CDS             complement(1344..2210)
                     /locus_tag="VFL04_09235"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET7839933.1"
                     /translation="MRDLEFFRNVSIGQYVDTGSAIHRMSPATKYLWLLALAIPAMAS
                     PGYAGALSPLLAALGRAAAARVSPGFLLRGLKPVVPLFAVVAVMQVLFGWPGDRSAVL
                     FRAGPIAATAREAHIVLMAVARTVSLVVVVGLFTSVTTEGEAAHGIEDFLAPFARLGF
                     PAHAFALAATTALRFIPIIAGELESIVKAQAARGADFGSGRGGLLAKARAYLPLFVPV
                     MIRALERAEALAEAMEARCYSGYSRTRYVVYEKLGGEWAARLLAPLFCAAVFILDSAL
                     SAPLAALLGAIS"
     gene            complement(2207..>2492)
                     /locus_tag="VFL04_09240"
     CDS             complement(2207..>2492)
                     /locus_tag="VFL04_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839934.1"
                     /translation="DLAARGVTVVMATHSMEEAARADFVAVVSRGRLAAFGLPREIFY
                     SSFDPDWGIGRPFACQLALELERLGVPVEGRPLEIEELEIPGPAAQDGRP"
BASE COUNT          423 a          847 c          927 g          295 t
ORIGIN      
        1 atgccgcggc ccagcgcgtc gcccgtcccg atgacgtcca ccgaggagga gccgcccgag
       61 ccgccatggg ccgcgccgag ctcctcgcgc atgaggccga cgaggcggga taccaggccc
      121 gagtagccga ggaggatccc cgactggatc gaggccgcgg tgttccggcc gatcgcgaaa
      181 cgcggcggtt cgagcctcac gtcggggatc tgggccgccg tcgagcggag ggcggcctgg
      241 gcggtctcga tgcccggggc gatggccgcg ccgaggaact cgcccgccgc gttgatcgcg
      301 gaaaaggcga tcgccgcccc gaagtcgacg acgacgcagg ctcccttcac gagctcccgc
      361 gccgccgccg cgacgcagac gaggtcggag ccgacctccg agggcaggtc cgtcctgatc
      421 ttcacccccg tcctcacccc gggcccgatg accgaggcct ggaggccgaa ggccgaggcg
      481 acggcctcga cgaggcgcgg cgtcagagcc ggcaccacgc tcgagatcca ggctgagtcg
      541 atggccttgc ccgaggcggg cgcggcctcg cgcaccgcct ggagcagaag ggcgtattcg
      601 tcggccgtcc gctcccggtc gctgcccagg cggcggattg tcagccaatc ccctccgtcc
      661 ctgaggccga cggtgacgga gcggttgcgg gcgtccaggg cgaggagcac ggcgcctcag
      721 ccctcgtcag cgagccgggc cttgccggtc ctgctctcga tgcccttcca agcggcgacc
      781 acggcgaggg taaggacgcc ggcgaccgcc gcctcgatga tgccgttggc cacgaagacc
      841 gaggcggcca ccgcgatggg gatcgccccg aagagcacga gggcgccgag gaccaggacc
      901 gagtgggtga tcgagccggc cacgccggcc acggtggccg caaggccggt catcctgccc
      961 cagaagaggc ggaagtaggc ccaggcggcg agcccgagga ggatcctcgg gaggacggag
     1021 atgagggggt tcacgaagaa gaggtcgatg gggccctggg gggcggtcgc cgccttgagg
     1081 agggccgtga ggccgaagat ggcccccacc gccatgccga cgacggggcc ctcgagcacc
     1141 gccgcgatga tgacgggcac gtggaggacg gtgatcgatg ccccggcgaa ccaggtgatg
     1201 aagcccaggt tgagcaggac gagggcgatc gaaaccgcgc cgagggctcc ggcgacgacg
     1261 accttcctga cgggcgatga tgttgccatg tgatgcctcc tacatgatca cggtatccgc
     1321 cgcgatgggt cggatccggt cattcacgag atggccccga gcagggctgc gagcggggca
     1381 gaaagcgcgg agtcgaggat gaagacggcg gcgcagaaga ggggggccag aagccgcgcc
     1441 gcccattcgc cgccgagctt ctcgtagacc acataacgcg tccgcgaata gcccgaatag
     1501 cagcgcgcct ccatggcctc ggccagggcc tcggcccgct cgagggcgcg gatcattacg
     1561 ggcacgaaga ggggcaggta ggcccttgcc ttcgccagga gccctcccct ccccgagccg
     1621 aagtcggccc cccgcgcggc ctgggccttc acgatggact cgagctcgcc ggcgataatg
     1681 gggatgaagc ggagggccgt cgtggcggcg agggcgaatg cgtgggcggg gaagccgagc
     1741 cgtgcgaagg gggcgaggaa gtcctcgatg ccgtgggccg cctccccctc ggtcgtcacc
     1801 gaggtgaaaa gccccacgac gacgacgagg gagaccgtcc tcgcgactgc catcagcacg
     1861 atgtgcgcct ctctcgccgt cgccgcgatc ggccccgccc tgaagaggac agccgaccta
     1921 tcccccggcc agccgaagag gacctgcatg accgcgacga cggcgaagag gggtacgaca
     1981 ggcttgagac cgcggaggag aaagccggga ctcacccgcg cggccgcggc acggccgagg
     2041 gcggcgagga ggggcgagag ggctccggcg tagcccgggg aggccatcgc ggggatggcg
     2101 agggcgagga gccagaggta cttcgtcgcc gggctcatcc ggtggatggc cgagccggta
     2161 tcgacatact gcccgatcga gacgttcctg aagaactcaa ggtccctcat gggcggccgt
     2221 cctgggccgc tggccccggt atctcaagct cctcgatctc gagcggcctc ccctcgaccg
     2281 ggacgccgag cctttccagc tcgagcgcca gctggcaggc gaaggggcgt ccgatgcccc
     2341 agtcggggtc gaagctcgag tagaagatct cccggggcag tccgaaggcg gccagcctcc
     2401 ccctcgagac cacggcgacg aaatcggcgc gcgccgcctc ctccatcgag tgggtggcca
     2461 tgacgactgt caccccacgt gcggcgaggt cg
//
LOCUS       DASSQV010000527         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_1115533, whole genome shotgun sequence.
ACCESSION   DASSQV010000527 DASSQV010000000
VERSION     DASSQV010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115533"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..679
                     /locus_tag="VFL04_09245"
     CDS             71..679
                     /locus_tag="VFL04_09245"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HET7839935.1"
                     /translation="MRKITCKCESSFEADLPEEIDLDSEPERVAQIIAGNFFAVTCPS
                     CGTELKPELRVRLSSRKARLDLTVLPELERLSVYRGKASLPKGSEAIIGYAELIERAR
                     IIADGLDAESVEIIKYWLAAKAEEPAPEAEILVAYAGQEAGKLTFHVRGLKDGELAVL
                     HVGRELYDKTLAEKARTLRSEPFERMFSGPYKSIRALEAELD"
     gene            complement(713..1009)
                     /locus_tag="VFL04_09250"
     CDS             complement(713..1009)
                     /locus_tag="VFL04_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HET7839936.1"
                     /translation="MGIRDDYDFSILTNEAERMVTDELERQLAARQAEQICVCEDCVI
                     DMAALALNSIRPVYRVSLLGTMYAHALDGGDFAEEVRRGVSSAIDRVHANPSHD"
     gene            complement(1045..2385)
                     /gene="dnaB"
                     /locus_tag="VFL04_09255"
     CDS             complement(1045..2385)
                     /gene="dnaB"
                     /locus_tag="VFL04_09255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET7839937.1"
                     /translation="MIPVQLKDKVPPHNAEAEQACLGAVLLDPEAVPEVLRFLRPEDF
                     YVNANREVFAGIISLYEKGQKADLITLTDEMRLLGSLEHAGGPGYVANLTSVTPSSAN
                     VEYYAKIVQETSIRRNLLKLSAEIGAKAHEDSMESGMILDEAQAKIFEITQNRQSVMY
                     KSVKELLPETIKTIEKLFKNKNAYTGIPSGLPDLDSMTSGFQDSEFIVIGARPSVGKT
                     ALALSMAAHIAVKERISCAFFSLEMADMAIMQRLISSEARISSEKIRSGLIKPSDFNA
                     LMEAAGRIYEAPLYIMDMPNMKLLDLRTMARRLRSERDVKIIFIDYLTLITHENSDLP
                     RWEQIASISRSLKALARELRIPVVALSQLKREAEGKQPTLADLRESGSIEQDADVILF
                     LHRDRETNKTRDERSPVVETELIIAKQRNGPVGKVEIAFLPSYTRFESIEKRMS"
     gene            complement(2564..3139)
                     /gene="rplI"
                     /locus_tag="VFL04_09260"
     CDS             complement(2564..3139)
                     /gene="rplI"
                     /locus_tag="VFL04_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET7839938.1"
                     /translation="MKVILNNDVPNLGELGDVKDVASGYARNYLFPRNLAYIFNSKTS
                     GMFQKRQAEIEALKNAKRQASLGLKEKLEAEEISITLPAGANGKLYGAVTSHSVADEL
                     LKKGIELDRKKIEVPGRSIKSVGNYKVLVRLYEKDEATIRLAIHGAAVKSDKPEEAPR
                     PQRRRRFEEHAEEATEQLAPEQAAPEQSPSD"
     gene            complement(3136..>3586)
                     /locus_tag="VFL04_09265"
     CDS             complement(3136..>3586)
                     /locus_tag="VFL04_09265"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HET7839939.1"
                     /translation="ASSYAVMLLAMIGGSWWIGCRLSGEGSPGRMAAGPATAYRVPYV
                     LVWPFLAFWAMVFAAILLRAPSWARAAAMNCALVLAAPYAIQGLGIVSHFLGRFNTPR
                     PLRIAIAATAVLALVTSTLGVVLAVALPILGVTEIWIPYRKTKGDGA"
BASE COUNT          585 a         1106 c         1201 g          694 t
ORIGIN      
        1 ctcgcgctct tcctcttcgc cacggtcggc gatgtcctat tcttcgcggc caaatagaaa
       61 ggggtgaacg atgcgaaaaa tcacatgcaa gtgcgaatcc agttttgaag ccgaccttcc
      121 ggaggagatc gacctcgact ccgagccgga gcgggtcgca cagatcatcg ccggcaattt
      181 cttcgcggtg acctgtccat cctgcggcac cgagctcaag cccgaacttc gggtgcgtct
      241 gagctcgagg aaggccaggc tcgacctcac ggtcctgccc gagctcgaga ggctctcggt
      301 ataccgcggc aaggcgagcc tccccaaggg ctcggaggcg ataatcggct acgccgagct
      361 gatcgagcgg gcgcgcatca tcgccgatgg cctcgacgcc gagtccgtcg agatcatcaa
      421 atactggctc gccgccaagg ccgaggagcc ggcgcccgag gctgagatcc ttgtcgccta
      481 tgcggggcag gaggcgggga agctgacctt tcatgtgcgg ggtctgaagg atggggagct
      541 cgccgtgctc cacgtgggca gggagcttta cgacaagacc ctcgccgaga aggcccgcac
      601 cctcaggtca gagcccttcg agaggatgtt ctcaggccca tacaagtcga tcagggccct
      661 cgaggccgaa ctggactagg gccacatgac ggggccgcgc ggcgcggccc cctcagtcgt
      721 gcgaggggtt ggcgtggacg cggtctatgg ccgacgagac gccccttctc acctcttcgg
      781 cgaaatcccc gccgtcgagg gcatgggcgt acatggttcc cagcaaggac acgcggtaga
      841 caggcctgat cgagttgagc gcaagagcgg ccatgtcgat gacgcaatcc tcacagacgc
      901 agatctgttc ggcctgccgg gcagcgagct ggcgctcaag ctcgtcggtc accatgcgct
      961 cggcctcgtt ggtgaggatg ctgaagtcgt agtcgtcccg gattcccatt gcccctcctt
     1021 ccagtgcctc actccactat agtttcacga catgcgcttt tcaatcgatt cgaaacgcgt
     1081 gtaggagggc aggaaggcga tctccacctt gcccacgggc ccgttgcgct gtttggcgat
     1141 gatgagctcg gtctcgacca cgggtgagcg ctcgtccctc gtcttgttcg tctcgcggtc
     1201 gcggtgaagg aagaggatga cgtcggcgtc ctgctcgatg gaccccgatt cgcggaggtc
     1261 ggcgagggtc ggctgcttgc cctcggcctc gcgcttgagc tgggagaggg ccacgacggg
     1321 gatgcggagc tcgcgggcca gggccttcag cgatcgggat atgctcgcta tctgctccca
     1381 tcggggcagg tcggagttct cgtgggtgat gagggtcagg tagtcgatga agatgatctt
     1441 cacatcgcgc tccgagcgga gccgcctcgc catcgtccgg aggtcgagga gcttcatgtt
     1501 cggcatgtcc atgatgtaaa gcggagcctc gtagatgcgg cccgcggcct ccatgagggc
     1561 gttgaagtcc gagggcttga tgaggcccga gcgtatcttc tcgctcgaga tcctggcctc
     1621 actggagatg aggcgctgca tgatggccat gtccgccatt tccagggaga agaaggcgca
     1681 gctgatgcgc tccttgaccg cgatgtgggc cgccatgctc agggcaaggg ccgtcttgcc
     1741 caccgaggga cgggcgccga tgacgatgaa ctccgagtcc tggaagcccg aggtcatcga
     1801 atcgaggtcg ggcaggcccg agggtatgcc cgtgtaggcg ttcttgttct tgaacagctt
     1861 ctcgatggtc tttatcgtct cgggcaggag ctccttgacg ctcttgtaca tgaccgactg
     1921 gcggttctgg gtgatctcga agatcttcgc ctgggcctcg tcgaggatca tgccggattc
     1981 catgctgtcc tcgtgggcct tcgcgccgat ctccgcggaa agcttgagga ggttccgcct
     2041 gatcgaggtc tcctggacga tcttggcgta atactcgacg ttcgccgacg agggggtcac
     2101 cgaggtcagg ttggccacat accctggccc gccggcgtgc tcgaggctcc cgaggagcct
     2161 catctcgtcg gtcagggtga tcaggtcggc cttctgcccc ttctcgtaca gggatatgat
     2221 gccggcgaag acctcccggt tggcgttgac gtagaagtcc tcgggccgaa ggaacctgag
     2281 cacctcgggc acggcctcgg ggtcgaggag gacggcgccg aggcaggcct gctcggcctc
     2341 ggcgttgtgc ggcgggacct tgtccttgag ctgtacggga atcattcgct ctcttcctcc
     2401 ggccccgggc ccgagtggat catagccccc catgggccga ccgcgctact gtgttcttgc
     2461 aggggctgat gcgcagcggg cgccaccctt cccggatggc gcccgccgcg gcatggaatc
     2521 ggaaggacgg ggccgggcgc tttcaggccc ggcccttggg gacctagtcg ctggggctct
     2581 gctccggggc cgcctgctcg ggcgcaagct gctcggtggc ctcctcggca tgctcctcga
     2641 aacggcgtct gcgctggggc ctcggggcct cctcgggctt gtcgctcttg acggcggcgc
     2701 cgtggatcgc gagcctgatc gtggcctcgt ccttctcgta gagcctgaca aggaccttgt
     2761 agttgccgac gctcttgatc gaccgtccgg ggacttcgat cttcttgcgg tcgagctcga
     2821 ttcccttctt gagcagctca tccgcgacgc tgtggcttgt cacggcgcca tagagcttgc
     2881 cgttcgcccc cgcgggcagg gtgatcgaga tctcctcggc ctcgagcttc tccttgagtc
     2941 caagcgaagc ctggcgcttc gcgttcttga gcgcctcgat ctcggcctgg cgcttctgga
     3001 acatgcccga ggtcttcgag ttgaagatgt aggcgaggtt cctggggaaa aggtaattcc
     3061 gggcgtagcc cgatgcgacg tccttgacgt ccccgagctc gccgagattc ggaacgtcgt
     3121 tattgagtat gactttcatg ctccgtctcc tttggtcttt cggtagggta tccagatttc
     3181 cgtgacgccg aggatgggca gggccaccgc cagtacgacg cccaaggtcg aggtcacgag
     3241 ggccagtacc gcagtcgccg cgatggcgat cctgagcggc cgtggcgtgt tgaaacgtcc
     3301 gagaaagtgg gaaacgatgc ccagtccctg gatcgcgtag ggggccgcga ggacaagggc
     3361 gcagttcatg gctgccgccc gcgcccagga gggggccctg aggaggatgg ccgcgaagac
     3421 catcgcccag aacgcgagga agggccagac caggacatat ggcactcgat aggccgtcgc
     3481 cggccccgcg gccatgcgac cggggctccc ttcaccggat agcctgcatc cgatccacca
     3541 gcttcccccg atcatggcga ggagcatgac ggcgtaggac gaggcg
//
LOCUS       DASSQV010000528         5860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_123551, whole genome shotgun sequence.
ACCESSION   DASSQV010000528 DASSQV010000000
VERSION     DASSQV010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123551"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /gene="rsgA"
                     /locus_tag="VFL04_09270"
     CDS             <1..590
                     /gene="rsgA"
                     /locus_tag="VFL04_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET7839940.1"
                     /translation="PGLPCVAICAPEGRGLASLSPWLEPGRTIVLLGSSGAGKSTLLN
                     ALAGRRLAETREVREDDQRGRHTTSHRQLYRLDSGALVIDTPGLREVQLWAEEATVDA
                     VYAEIEGQAELCRFRDCSHENEPGCAVRAALETGEIERGRYEGWRKLRAELRFLRARE
                     DPALRKANAEKWKTINKAMRGYSKERRASAGKSFG"
     gene            complement(587..1639)
                     /locus_tag="VFL04_09275"
     CDS             complement(587..1639)
                     /locus_tag="VFL04_09275"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET7839941.1"
                     /translation="MEKIGVIGAGAWGTALAKVLADCGHEVELWCLEPEVAASIRERN
                     ENEKYLPGVKLPPSLVPETDVLAAASGKDVVLLAVPSPWLMGVVRRILPAPGIMEGES
                     LIVVVTKGFIPGPRGPRLILDALEDHLPGFYRGGLVYLSGPSHAEEVSRGRITGLAAA
                     SQNGRNAIRVRELFRGSRVLVFPSLDTTGVQVCAASKNVVAIAFGMLDALAADDPDFG
                     DNTESLLLAAGLNEMQTLGVALSATHPETFTSIAGVGDLDVTCRSVHGRNRRFGREIV
                     GKGILAPFRDLDDLLARIAEVGYLPEGAVACRQVAALAASLKLELPIMGGVHSILDKR
                     VEPLAFLEAYLASQGA"
     gene            complement(1650..2099)
                     /gene="ndk"
                     /locus_tag="VFL04_09280"
     CDS             complement(1650..2099)
                     /gene="ndk"
                     /locus_tag="VFL04_09280"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET7839942.1"
                     /translation="MARERTFSMLKPGVVQRRLAGEILARIERKGLRIIALKMMKIDR
                     KLAETHYGEHRDKPFFGELVDFIISGPVVAMVVEGDGAVSVLRKLCGATKAEEALPGT
                     IRGDYAEHTGLNIIHASDSVQSAEREIGLFFRLEELFDWEDGNDGWI"
     gene            2251..>5860
                     /locus_tag="VFL04_09285"
     CDS             2251..>5860
                     /locus_tag="VFL04_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HET7839943.1"
                     /translation="MRLARRLGIDVGSTTVKLALLGEGGSVLFGRYERHRSDVSTTLS
                     RLLEEASAALGALGRDVRATVTGSAGIGTAERLGLPFVQEVMACSRAVERLLPGAEVA
                     VELGGEDAKITYFGDRLEQRRNGSCAGGTGAFIDQMAVLLGTDPAGLDALASGATCIY
                     PIAARCGVFAKADVQPLVNEGARREDIAASVLQAVVNQSISGLACGRPIRGRVAFLGG
                     PLHFLPELRRRFVQTLGLKAEEILVPEDGRLFVALGAALHARESSPELPCSLAELASR
                     SRASKPVGEAPRMPPLFSSPPERAEFQARHGASSLPLGEIGEAAGPVFLGIDAGSTTT
                     KLALIDGEARLLHSFYAPNGGHPLSVLSEALGRLYAALPAAAWIGRSCATGYGESLVK
                     AALGADEGEVETVAHCRAAEALLPGVESVLDIGGQDMKFIRVKDGVIASVLLNEACSS
                     GCGSFLETFAHSLGYGSAEFAALALESASPVDLGSRCTVFMNSRVKQAQKEGATPADI
                     AAGLAYSVVRNALQKVIRLKRPEDVGSRVVVQGGTFASEAVLRCFELVSGARAMRPAQ
                     TGLMGAYGAALVARSHWREGARSTMAGRAELGGFGYSTESRRCPGCANACLLTVTSFG
                     SGARPHVGGNRCERGATLAIAECGAAHTITDRGPGGPRRAPADAAPIVGAAPTVGAAT
                     FAGAATKVGAAAAAARSAPAPTEVPFEALPPDLFAWKHGRIFAGEALPPERASRGRLG
                     IPRVLNLYENYPFWQGLLTSLGFSVALSPRSSKSLAERGMDTIPSESICYPAKLAHGH
                     GASLAVSGSGLVFYPCVPRERRFVAGSDNRFNCPVVTSYPEVLLNNIEAFRDGSMPYL
                     NPFLPIDSRSRLAARIVEEFATWEVTPREAKAAVEAAHAAQEAYGAELRARGEAALDW
                     VSRRGAHGIVLAGRPYHVDPEVHHGIPGIITGLGMAVLSEDSICHLGRVERPLRVVDQ
                     WAYHSRLYAAASFVSGRDELDLVQLVSFGCGLDAVTSDQVEEILARRGRIYTAIKIDE
                     HANLGAARIRMRSLAAAIADREAAGRRATAPAPLPGRRSFSKAMRAEYSILAPQMSPI
                     HFRLVEAAFRASGFRLEILPVAGREELDEGLRAVNNDACFPSILVTGQLMAALRSGRY
                     DLDRTALLVSQTGGGCRASNYIAFIRKALADSGLERLPVISLNALGMESHPGFRLSP"
BASE COUNT          825 a         2138 c         2046 g          851 t
ORIGIN      
        1 cgccgggcct gccctgcgtc gcgatatgcg cccccgaggg caggggcctg gcctcgctct
       61 cgccctggct cgagccgggc aggaccatcg tcctcctcgg ctcctcgggc gccggcaagt
      121 cgaccctcct caacgccctc gccgggaggc gtctggccga gacgagggag gtgcgcgagg
      181 acgaccagag gggccgccac accacgagcc accgccagct ctaccgcctc gactcgggcg
      241 ccctcgtgat cgacaccccc ggactccgcg aggtccagct ctgggccgag gaggccacgg
      301 tggacgccgt ctacgccgag atcgagggcc aggccgagct ctgccgtttc cgcgactgca
      361 gccacgagaa cgagcccggc tgcgccgtgc gcgccgccct cgagacgggg gagatcgaga
      421 ggggccgcta cgagggctgg aggaagctgc gcgccgagct caggttcctc cgggccaggg
      481 aagacccggc cctgcggaag gccaacgccg agaagtggaa gaccatcaac aaggccatgc
      541 ggggctattc gaaggagcgc agggcgagcg ccgggaagtc cttcggctag gccccctgcg
      601 aggcgaggta ggcctcgagg aaggccaggg gctccacgcg tttgtcgagg atcgagtgga
      661 cgccgcccat gatgggcaac tcgagcttga gcgaggccgc gagggcggcg acctgtctgc
      721 aggcgacggc gccctcgggg aggtagccga cctcagctat cctcgcgagg aggtcgtcga
      781 ggtccctgaa gggcgccagg atgcccttgc cgacgatctc gcggccgaag cggcggttgc
      841 gcccgtgcac cgagcggcag gtgacgtcga ggtccccgac cccggcgatc gaggtgaagg
      901 tctcgggatg ggtggcgctg agggccacgc cgagggtctg catctcgttg aggccggcgg
      961 cgaggagcag tgactcggtg ttgtcgccga agtcggggtc gtcggcggcg agggcgtcga
     1021 gcatgccgaa ggctatcgcg acgacgttct tggaggcggc gcagacctgg acccccgtgg
     1081 tgtcgagcga ggggaagacg aggacccgcg agccgcggaa gagctcccgc acccggatgg
     1141 cgttgcgccc gttctggctg gcggcggcga ggcccgtgat gcggcctcgc gagacctcct
     1201 cggcgtggct cggaccggaa aggtacacga ggccgccgcg gtagaagccg ggcaggtggt
     1261 cctcgagggc gtcgaggatg aggcgggggc cgcggggccc cggtatgaag cccttggtga
     1321 cgaccacgat gagggactcg ccctccatga tccccggcgc gggaaggatc ctccgcacga
     1381 cgcccatgag ccagggcgag ggcaccgcca acagcacgac gtccttgccc gaggccgcgg
     1441 cgagcacgtc ggtctccggc acgaggctcg ggggaagctt gacccccggc aggtacttct
     1501 cgttctcgtt ccgctcgcgg atcgaggcgg ccacctcggg ctcaaggcac cagagctcga
     1561 cctcgtggcc gcagtcggcg aggaccttgg cgagggccgt gccccaggct ccggcgccga
     1621 tgactcctat cttctccatg cctgcccttt cagatccagc cgtcgttgcc gtcttcccag
     1681 tcgaaaagct cctcgagacg gaagaaaagg cctatctcgc gctcggcgct ctggacggaa
     1741 tccgaggcgt ggatgatgtt gagcccggtg tgctcggcgt agtcgccgcg gatggtgccg
     1801 ggcagggcct cctcggcctt ggtggctccg cagagcttgc gcaggacgct caccgcgccg
     1861 tcgccctcga cgaccatggc caccacgggt ccggaaatta tgaagtccac gagctcgccg
     1921 aagaatggct tgtcgcggtg ctcgccatag tgggtctcgg cgagcttccg gtctatcttc
     1981 atcatcttga gcgcgatgat gcgaaggccc ttgcgttcta tcctcgccag tatctctccg
     2041 gcgaggcggc gttgcacgac gcccggcttg agcatgctga aagtgcgttc tcgcgccatg
     2101 ttccctccgt ccccgggata gtagcacggg gagcccggat cggaaaggcg ggcggggccg
     2161 atgcggggcc ccggggcgag actggggcgg gcccgggagg cggtccctgt cttgccccgg
     2221 gccacggggg gatgtatcct tgcgcccgaa atgaggctcg cgcgaaggct cgggatcgac
     2281 gtcggctcga ccacggtgaa gctcgccctc cttggcgagg gcggctcggt cctgttcggg
     2341 cgctacgagc gccaccgctc cgacgtgagc acgaccctct cccgcctcct cgaggaggcc
     2401 tcggccgccc tcggggccct ggggagggat gtgcgggcga cggtcacggg ctcggccggg
     2461 atcggcacgg ccgagcgcct cggcctgccc ttcgtccagg aggtcatggc ctgctcccgc
     2521 gcggtcgagc gcctcctgcc gggggccgag gtggccgtcg agctcggggg cgaggacgcc
     2581 aagatcacct atttcggcga ccgcctcgag cagaggagga acggcagctg cgcggggggc
     2641 acgggggcct tcatcgacca gatggccgtc ctcctcggca ccgatcccgc cggcctcgac
     2701 gccctcgcct cgggcgccac ctgcatctac cccatcgccg cgcgctgcgg ggtcttcgcc
     2761 aaggccgacg tccagcccct cgtgaacgag ggggccagga gggaggacat cgcggccagc
     2821 gtcctccagg ccgtggtgaa ccagtcgatc tcgggcctcg cctgcggaag gccgatcagg
     2881 ggcagggtcg ccttcctagg cgggcccctg cacttcctcc ccgaactcag gcggcgcttc
     2941 gtccagaccc tcggcctcaa ggccgaggag atcctcgtgc ccgaggacgg ccggctcttc
     3001 gtcgccctgg gcgccgccct gcatgcccga gagtcctcgc ccgagctccc ctgcagcttg
     3061 gccgagctgg cctcgaggtc tcgtgcctcg aagcctgttg gcgaggcgcc gaggatgccc
     3121 ccgctcttct cctcgcctcc cgagcgagcc gagttccagg cgaggcacgg cgcgagctcc
     3181 ctgcccctcg gcgagatcgg ggaggccgcg ggtccggtct tcctcgggat cgacgcgggc
     3241 tcgaccacga ccaagctggc cctgatcgac ggtgaggcca ggctcctcca ctccttctac
     3301 gcccccaacg gcggccaccc cctctcggtc ctgtccgagg ccctggggcg gctctacgcc
     3361 gccctgcccg cggcggcctg gatcgggcgc tcgtgcgcca caggctacgg cgagtccctc
     3421 gtgaaggcgg ccctcggcgc cgacgagggc gaggtcgaga ccgtggccca ctgccgagcc
     3481 gccgaggccc tcctgccagg ggtcgagtct gtcctcgaca tcggcggcca ggacatgaag
     3541 ttcatcaggg tgaaggacgg ggtgatcgcc tccgtcctcc tcaacgaggc ctgctcctcg
     3601 ggctgcggct ccttcctcga gaccttcgcc cactcgctcg gctacggctc cgcggagttc
     3661 gcggccctgg ccctcgagtc ggcctcgccc gtggacctgg gcagccgctg caccgtcttc
     3721 atgaactcga gggtgaaaca ggcccagaag gagggggcca cgccggccga catcgccgcc
     3781 ggcctggcct attccgtcgt ccgcaacgcc ctccagaagg tgatccgcct caagcgcccc
     3841 gaggacgtgg gctcgcgggt tgtggtccaa ggggggacct tcgcgagcga ggccgtcctg
     3901 cgctgtttcg agctggtctc gggggccagg gccatgaggc cggcgcagac cgggctcatg
     3961 ggcgcctacg gggccgccct cgtcgcccgc tcccactgga gggaaggggc aaggtcgacc
     4021 atggcaggca gggccgagct cggcggcttc ggctacagca ccgagtcccg gcgctgtccg
     4081 ggctgcgcca acgcctgtct ccttacggtc acgagcttcg gaagcggggc caggccccac
     4141 gtaggcggca accgctgcga acgcggcgcc acacttgcga tcgcggagtg cggcgccgca
     4201 catacaatca cggatcgcgg gcctggtggg ccccggaggg cgccggctga tgcggcgccc
     4261 atcgtaggcg ccgcgcccac agttggggcg gcgacctttg cgggggctgc gaccaaagtt
     4321 ggggcggcgg ccgcggccgc gcggtcagcc ccggcgccca ccgaggtccc gttcgaggcc
     4381 ctcccccccg acctcttcgc ctggaagcac gggcggatct tcgcgggcga ggccctcccc
     4441 cccgagcggg cgagccgggg caggctcggc atcccgaggg tcctcaacct ctacgagaac
     4501 taccccttct ggcagggcct cctcacgagc ctcggcttca gcgtggccct aagcccccgc
     4561 tcatcgaaaa gcctcgccga gcggggcatg gacacgatcc cctccgagtc gatctgctat
     4621 ccggccaagc tcgcccacgg ccacggagcc agcctcgccg tctcgggctc gggcctcgtc
     4681 ttctacccct gcgtcccccg cgagcgacgc ttcgtcgccg gttcagacaa ccgcttcaac
     4741 tgccccgtgg tcacctccta tcccgaggtc ctcctcaaca acatcgaggc cttcagggac
     4801 ggcagcatgc cctacctgaa ccccttcctc cccatcgact cccgcagccg tctggcagcg
     4861 cgcatcgtcg aggagttcgc cacctgggag gtcacgcccc gcgaggccaa ggccgccgtc
     4921 gaggccgccc atgcggccca ggaggcctac ggggccgagc tcagggcccg cggcgaggcc
     4981 gccctcgact gggtctcgcg gaggggggcc catggcatcg tcctcgccgg ccggccctac
     5041 cacgtcgacc ccgaggtcca ccacggcatc cccgggatca tcacgggcct cggcatggca
     5101 gtcctgagcg aggactcgat ctgccacctc ggcagggtcg agcgccccct gcgcgtcgtc
     5161 gaccagtggg cctaccactc gaggctctac gcggcggcct ctttcgtctc gggccgcgac
     5221 gagctcgacc tcgtgcagct cgtgtccttc ggctgcggcc tcgacgcggt gacctcggac
     5281 caggtcgagg agatcctcgc tcggcgcggg aggatctaca ccgcgatcaa gatcgacgag
     5341 cacgccaacc tcggcgccgc ccgcatcagg atgcgctccc tggccgcggc catagccgac
     5401 cgcgaggccg cggggcgccg cgccacggcc cccgcccccc tgcccgggcg gaggagcttc
     5461 agcaaggcga tgcgcgccga gtacagcatc ctcgccccgc agatgtcgcc catccacttc
     5521 cgcctcgtcg aggcggcctt cagggcctcg ggcttccgcc tcgagatcct tccggtcgcc
     5581 ggccgagagg agctcgacga gggcctgcgc gcggtgaaca acgacgcctg cttcccctcg
     5641 atcctcgtca cgggccagct gatggccgcc ctccgctcgg gccgctacga cctggaccgc
     5701 acagccctcc tcgtgtccca gaccggcggg ggctgcaggg cctcgaacta catcgccttc
     5761 atacgcaagg ccctcgccga ctcggggctc gagcggctgc cggtgatttc gctcaacgcc
     5821 ttgggcatgg agagccaccc gggcttccgc ctctcgcccg
//
LOCUS       DASSQV010000529         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_123729, whole genome shotgun sequence.
ACCESSION   DASSQV010000529 DASSQV010000000
VERSION     DASSQV010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123729"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VFL04_09290"
     CDS             <1..836
                     /locus_tag="VFL04_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HET7839944.1"
                     /translation="HGVKLLLLHGGPGSTHDYLEAFDAFLPAAGIEYYYYDQLGAGKS
                     DSPDEASLWDLGRFVDELEQVREALGLDARNLCLYGHSWGGILAIEYALAHQDRLKAL
                     VVSNMMASIASYNAYAERVLAPQIESGVLAEVRRLEAEGRTEEPRYMELLIPHFYERH
                     ILRMPEADWPEPVKRAFALENRRIYVPMQGPSELGASGALLGWDRFDELARIEVPALV
                     IGAEHDTMESEHLRSMASRLPRGRYHHCPEGSHLAMWDDQETYFRGLLGFIGDVEAGR
                     I"
     gene            1096..1479
                     /locus_tag="VFL04_09295"
     CDS             1096..1479
                     /locus_tag="VFL04_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7839945.1"
                     /translation="MKRVTGIGGIFFTAKDPVALRAWYRDHLGIEVQEWGGAIFEWAA
                     EGQTIPGTTVWTINAAGGGEFAPSKASFMVNYRVEDLDALLRALRDEGCEVLDRRDDS
                     EYGKFGWVMDPEGNKVELWQPPEGR"
     gene            1490..2035
                     /locus_tag="VFL04_09300"
     CDS             1490..2035
                     /locus_tag="VFL04_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839946.1"
                     /translation="MVILIGGTAGVGKTLLARRIMERSGIPFLPVDHLMMGIYRSDPA
                     CGFTPESDVQRISDALWPILFGMIKTSIENGLGYTYEGFQILPGKLASIGSEYRAEIR
                     SAFPCLGEEYIESRYETIRLRRNAVERRADLETKEEMLQRNREFIAACEASGIRPFVI
                     RRDYEAELEVILEAIVGPALG"
     gene            2080..>2343
                     /locus_tag="VFL04_09305"
     CDS             2080..>2343
                     /locus_tag="VFL04_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839947.1"
                     /translation="MLSHPLKDDCHLAVIEKRHAAAFFAFIEKNRAHYKGVLRFIEKL
                     TSVKEVEAFILRGQHAAADGSFCSWGIWDGRTIVGVVSVRDMDE"
BASE COUNT          447 a          745 c          781 g          370 t
ORIGIN      
        1 gtcacggggt aaagctcctc ctcctccacg gaggccccgg ctcgacccac gattacctcg
       61 aggccttcga cgccttcctc cccgccgcgg ggatcgagta ctactactac gaccagctcg
      121 gcgcgggcaa gagcgacagc cccgacgagg cctcgctctg ggatctcggc cgcttcgtcg
      181 acgagctcga gcaggtgcgg gaagccctcg gccttgacgc gcgaaacctt tgcctctacg
      241 gccactcctg gggtggcatc ctcgccatcg agtacgccct cgcccaccag gaccggctca
      301 aggccctcgt cgtgtcgaac atgatggcga gcatcgcttc ctacaacgcc tatgccgagc
      361 gtgtcctcgc gccccagatc gagagcgggg tcctggccga ggtgaggcgc ctcgaggccg
      421 agggcaggac cgaggagccg cgctacatgg agctcctcat cccccacttc tacgagcgcc
      481 acatccttcg aatgcccgag gccgattggc ccgagccggt gaagcgcgcc ttcgccctgg
      541 agaaccggag gatctacgtc cccatgcagg gcccgagcga gctcggagcc tccggcgccc
      601 tcctcggctg ggaccgcttc gacgagctcg cgcggatcga ggtgcccgcc ctcgtcatcg
      661 gggctgagca cgacacgatg gagagcgagc atctcagatc gatggcctcc cgcctgcccc
      721 gaggccgcta ccaccactgc cccgagggtt cccacctcgc gatgtgggac gaccaggaga
      781 cctacttccg cggcctcctt ggcttcatcg gggatgtcga ggcggggagg atctaggagg
      841 gaggctccca atccggagcc tggattcccg aacggtgata ggagaccgct tcccggctcg
      901 tgagggagca gcgggttagg cggcggaagc tgagtgagat tatcgcgcat tagaatggga
      961 atgaggggcg gagaagtctg ggggatgctg acaaccgacc tgacctgcag aaagtactag
     1021 aggtcgcacc tgcgagcgga gatagtcagt gaggtcacta cggcaggcgg actgccccaa
     1081 agcgaggagg aaaccatgaa acgcgtcacc ggcatcggcg gcatcttctt caccgccaag
     1141 gaccccgtcg cccttcgagc ttggtacagg gaccacctcg gcatcgaggt ccaggaatgg
     1201 ggaggggcta tcttcgagtg ggccgccgag ggccagacca tcccggggac gaccgtgtgg
     1261 accatcaacg cggcaggggg cggagaattc gcgcccagta aggccagctt catggtcaac
     1321 taccgcgtcg aggacctcga tgccctgctc agggcactcc gcgacgaggg ctgcgaggtc
     1381 ctggacagga gggacgactc ggagtacggc aagttcggct gggtcatgga ccccgagggg
     1441 aacaaggtcg agctctggca gccgcccgag gggcggtgaa ggcgactcca tggtcatcct
     1501 gatcggcggc acggcgggcg tcgggaaaac cctcctcgcc cgccgcatca tggagaggag
     1561 tgggatcccc ttcctccccg tcgaccacct gatgatgggc atctaccgga gcgaccccgc
     1621 ctgcggcttc acgcccgaaa gcgatgtcca gcggatctcc gatgccctct ggccgatcct
     1681 cttcgggatg atcaagacca gcatcgagaa cggtctcggc tacacgtacg agggcttcca
     1741 gatcctgccc gggaaactcg cgtcgatcgg cagtgagtac cgggccgaga tacggtcggc
     1801 cttcccctgc ttgggcgagg agtatatcga atcgcgatac gagacgatca ggctccggcg
     1861 gaacgcggtg gagcggcgag ccgacctcga gaccaaggag gagatgctgc agaggaaccg
     1921 cgagttcatc gctgcttgcg aggcgagtgg aatacggccc ttcgtcatca ggcgggacta
     1981 cgaggccgag ctcgaggtga tcttggaggc gatcgtcggg ccggcactag ggtaaaatgg
     2041 tccatcggta cgcctgaact cgagttccag gaggacatca tgctaagcca tccactcaag
     2101 gacgactgtc acctcgcggt catcgaaaag cgccacgctg cggctttctt cgccttcatc
     2161 gagaagaacc gcgcgcacta caaaggcgtc ctgcgcttca tcgagaagct cacgagcgtg
     2221 aaggaggtcg aggccttcat ccttaggggc cagcacgcgg cggccgacgg aagcttctgc
     2281 tcctggggga tctgggacgg caggacgatc gtcggggtcg tgagcgtgcg ggacatggac
     2341 gag
//
LOCUS       DASSQV010000530         3682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rectinemataceae bacterium isolate SMAG_U472
            k141_745725, whole genome shotgun sequence.
ACCESSION   DASSQV010000530 DASSQV010000000
VERSION     DASSQV010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968500
            Sequence Read Archive: SRR25158363
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinemataceae bacterium (soil metagenome)
  ORGANISM  Rectinemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:00:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,864
            CDSs (total)                      :: 1,848
            Genes (coding)                    :: 1,844
            CDSs (with protein)               :: 1,844
            Genes (RNA)                       :: 16
            tRNAs                             :: 16
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Rectinemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745725"
                     /isolate="SMAG_U472"
                     /isolation_source="soil"
                     /db_xref="taxon:3064674"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VFL04_09310"
     CDS             <1..572
                     /locus_tag="VFL04_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7839948.1"
                     /translation="KEFLETMILCLDLRSFSSYARDEGRDEVKGFLERYTQELLAAIN
                     AYPVSYYKLLGDGALILWDRPGRGDVDNAVELFTLLRWVIAETGPSYGYHNSLAGALL
                     VDEVYKYEIYAESSGLKYRDYVGYGINFAFRLQGAARSKELLCTRDLVDRFGLAASPL
                     APDARPTSESMKGIRERDYEDIQVLVAPD"
     gene            complement(587..1783)
                     /gene="ygeW"
                     /locus_tag="VFL04_09315"
     CDS             complement(587..1783)
                     /gene="ygeW"
                     /locus_tag="VFL04_09315"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HET7839949.1"
                     /translation="MTKSEIGKLIESLRTKKLGMYHKDFLLTWEKSQSELEATFIVAD
                     VLRAMRDAGISPKVWDSGIAVSNFRDNSTRTRFSFASACDLLGLYIQDLDEGKSQIAH
                     GETVRETANMISFLSEAIGIRDDMYLGEGDKYQREVGKALDEGLAAGVLPDRPTVVNL
                     QSDIDHPTQSMADFLHLSHHFGGLDKLKGKKIAMTWAYSPSYGKPLSVPQGIIGLASR
                     LGMEITLAHPEGYDLIPEVVELSKRQAKESGGSFKHVNSMDEAFRDADIVYPKSWAPF
                     DVMQKRVPLLKNGDQAGLKELEKQCLANNARFKDWECTEAKMKLTKGGKALYMHCLPA
                     DITDVSCAAGEVAASVFDRYRDDTYREASYKPYIIAAMILLGKSRDPAATLSRLLDRN
                     AARSQL"
     gene            complement(1841..>3682)
                     /locus_tag="VFL04_09320"
     CDS             complement(1841..>3682)
                     /locus_tag="VFL04_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7839950.1"
                     /translation="YQFPLHAEPHRNLAASVADGIGDIDKGFAEADVVIERTYKGNRV
                     QCTPMEPHVVYAKIDNGRLVIHASTQVPWHLRRIVATAIGISENKVRVIKERVGGGFG
                     AKQDIVLEDVAAYLAWKTGRPVFSRYSREEEFIASRTRHLMEVTVKLGAKRDGRLTAI
                     LMRLDADTGPYGQHCLTVPMNAISKSLPLLLCDNARFEVKSYYTNLSPAGAYQGYGAP
                     KGSFALQTALAELADELGLDQLELIEKNRVRSGARIEILKSLGEGRPGAAVTLGECGL
                     GEMLARGSKAFDWGKPLPSGEDPDWRRGRGAVIIQQGSGLPGLDTANAELRLLRDGTV
                     LMLSGGADLGTGLDTLTSKAAAEILGLDMEDVAVISGDTDITPFDKGAYASSGTFFSG
                     SAAVRAAEDLRDKILATAAGILGESVADLRIAHRGMVKGRKGEISLAQLAHMSTQGEH
                     HGELVGFGNFKTEHAAFPYGAHFVQVAVNGRTGEVRLERYHAYQDCGTPINPALAIGQ
                     IYGGVLKAIGHSLYEEMLYDDEGRCLNTGFLDYKIPTINEIPEDFHVELVPVADEVGP
                     FGGKSVSEISLNGAAPAISIAIHDAVKVWLREWPFTPERVLRAMGKL"
BASE COUNT          544 a         1165 c         1310 g          663 t
ORIGIN      
        1 agaaggagtt cctcgagacg atgatcctct gcctcgacct gcggagcttc tcgagctacg
       61 cgcgcgacga gggcagggac gaggtgaagg gcttcctcga gcgctacacc caggagctgc
      121 tcgcggccat caacgcctac cccgtctcct actacaagct attgggggac ggggcgctta
      181 tcctctggga caggcccggc aggggggacg tcgacaacgc cgtggagctc ttcaccctcc
      241 tgcgctgggt catcgccgag acggggccga gctacggcta ccacaacagc ctggcgggcg
      301 ccctgctggt ggacgaggtc tacaaatacg agatctacgc cgagtcctcg ggcctgaagt
      361 accgcgacta cgtcggctac gggatcaact tcgccttcag gctccagggg gccgcgaggt
      421 ccaaggagct gctctgcacg agggacctgg tggaccgctt cggcctcgcg gcgagccccc
      481 tggcccccga cgccaggccc acgagcgagt cgatgaaggg gataagggaa agggactacg
      541 aggacatcca ggtcctcgta gcccccgact gaagaagggc ctcggcctag agctggcttc
      601 tcgcggcgtt cctgtcgagg agccgcgaca gggtcgccgc cgggtccctg gacttaccga
      661 ggaggatcat cgccgcgatg atgtagggct tgtagctcgc ctcgcggtag gtgtcgtcgc
      721 ggtagcggtc gaagacgctg gcggcgacct cgcccgcggc gcaggacacg tcggtgatgt
      781 cggcaggcag gcagtgcatg tagagggcct tgcccccctt ggtgagcttc atcttcgcct
      841 cggtgcactc ccagtccttg aagcgcgcgt tgttcgcgag gcactgcttc tcgagctcct
      901 tcagacccgc ctggtccccg ttcttgagca ggggaacccg cttctgcatg acgtcgaagg
      961 gggcccagct cttcgggtag acgatgtcgg cgtccctgaa ggcctcgtcc atcgagttga
     1021 cgtgcttgaa tgagcctccc gattccttgg cctggcgctt tgagagctcg accacctcgg
     1081 ggatgaggtc atagccctcg gggtgggcga gggtgatctc catgccgagc cggctcgcga
     1141 gcccgatgat gccctggggc accgagaggg gcttgccgta ggagggggag taggcccagg
     1201 tcatggcgat cttcttgccc ttgagcttgt cgagcccgcc aaagtggtgg gagaggtgga
     1261 ggaagtcggc catggactgg gtggggtggt cgatgtcgga ctggaggttg accacggtgg
     1321 ggcggtcggg caggacgccg gcggcgaggc cctcgtcgag ggccttgccg acctcccgct
     1381 ggtacttgtc gccctcgccg aggtacatgt cgtcgcggat gccgatggcc tcggagagga
     1441 agctgatcat gttggcggtc tcgcgcacgg tctcgccatg ggcgatctgg ctcttgccct
     1501 cgtccaggtc ctggatgtag agccccagga ggtcgcaggc gctcgcgaag gagaagcggg
     1561 tcctcgtgga gttgtcgcgg aaattggaga cggcgatccc cgagtcccag accttggggg
     1621 agatgccagc gtcgcgcatc gcgcggagca cgtcggcgac gatgaaggtc gcctcgagct
     1681 cggactggct cttctcccag gtcaggagaa agtccttgtg gtacatgccg agcttcttgg
     1741 tcctcaggct ctcgatcagc ttgcctatct cgctcttcgt catctcttct tcctccgctc
     1801 atccatatcg tcggccccac agggggccgg tttcgtactg ctacagcttc cccatggccc
     1861 gcagcacccg ctcgggcgtg aagggccatt cgcggagcca gaccttgacg gcatcgtgga
     1921 tcgcgatgga gatcgcgggc gccgcgccat tgagcgagat ctccgacacg ctcttgccgc
     1981 cgaagggacc cacctcgtcc gcgacgggca cgagctcgac gtggaagtcc tcgggtatct
     2041 cgttgatggt ggggatcttg tagtcgagga agccggtgtt gaggcagcgg ccctcgtcgt
     2101 cgtagagcat ctcctcgtag agggagtggc cgatggcctt gagcacaccg ccgtagatct
     2161 gtcctatggc gagggccggg ttgatcggcg tgccgcagtc ctggtaggcg tggtagcgct
     2221 cgagcctcac ctcgcccgtc cggccgttga ccgccacctg gacgaagtgg gcgccgtagg
     2281 ggaaggccgc gtgctcggtc ttgaagttgc cgaagcccac gagctcgccg tggtgctcgc
     2341 cctgggtgga catgtgggcg agctgggcca ggctgatctc gcccttcctg cccttcacca
     2401 tgccgcgatg cgcgatccga aggtcggcca cgctctctcc gaggatgccg gcggccgtgg
     2461 cgaggatctt gtcgcggagg tcctcggcgg cgcggacggc ggcgctgccc gagaagaagg
     2521 tgcccgagga ggcgtaggcc cccttgtcga agggggtgat gtcggtgtcg cccgagatca
     2581 ccgccacgtc ctccatgtcg aggcccagga tctcggccgc cgccttggac gtgagggtgt
     2641 cgaggccggt gccgaggtcg gccccgcccg agagcatgag caccgtgccg tcgcgcagga
     2701 gcctgagctc ggcgttggcc gtgtcgaggc cgggcaggcc cgagccctgc tggatgatga
     2761 cggctccgcg gccacgcctc cagtccgggt cctcgcccga gggcaggggc ttgccccagt
     2821 cgaaggcctt cgagccccgg gccagcatct cgccgagccc gcactcgccg agggtcacgg
     2881 ccgcccccgg cctgccctcg ccgaggctct tgaggatctc gatgcgggcc cccgagcgca
     2941 cgcggttctt ctcgatcagc tcgagctggt cgaggccgag ctcgtcggcc agctcggcca
     3001 gggcggtctg gagggcgaag gagcccttgg gggcgccgta gccctggtag gcgccggcgg
     3061 gcgagaggtt cgtgtagtag gacttcacct cgaagcgcgc gttgtcgcac agaaggaggg
     3121 gaaggctctt cgagatcgcg ttcatgggga cggtgaggca gtgctggccg tagggccccg
     3181 tgtcggcgtc gagccgcatg aggatggcgg tgagcctgcc gtcgcgtttg gcgccgagct
     3241 tcacggtcac ctccatgagg tgcctcgtgc ggctcgcgat gaactcctcc tcgcggctgt
     3301 agcgggagaa gaccggcctg cccgtcttcc aggcgaggta ggcggccacg tcctcgagga
     3361 cgatgtcctg tttggcgccg aagccgccgc ccaccctctc cttgatcacg cgcaccttgt
     3421 tctcgctgat cccgatcgcg gtggccacga tgcggcgaag gtgccagggc acctgggtcg
     3481 aggcgtggat gacgaggcgc ccgttgtcga tcttggcgta gaccacgtgg ggctccatcg
     3541 gcgtgcactg cacccggttg cccttgtagg tcctctcgat cacgacatcg gcctcggcga
     3601 aacccttatc gatgtcgcct atcccgtcgg ccacgctggc ggcgaggttg cggtggggct
     3661 cggcgtggag ggggaactgg ta
//
